LOCUS       DAPPNJ010000001        61064 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000001 DAPPNJ010000000
VERSION     DAPPNJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.001"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..513
                     /locus_tag="RPM73_000001"
     CDS             <1..513
                     /locus_tag="RPM73_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDX6518401.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            529..1068
                     /locus_tag="RPM73_000002"
     CDS             529..1068
                     /locus_tag="RPM73_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDX6518402.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="RPM73_000003"
     CDS             complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="RPM73_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDX6518403.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="RPM73_000004"
     CDS             complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="RPM73_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDX6518404.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4435..5805
                     /gene="xseA"
                     /locus_tag="RPM73_000005"
     CDS             4435..5805
                     /gene="xseA"
                     /locus_tag="RPM73_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDX6518405.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5802..6017)
                     /locus_tag="RPM73_000006"
     CDS             complement(5802..6017)
                     /locus_tag="RPM73_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDX6518406.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6086..7558)
                     /gene="der"
                     /locus_tag="RPM73_000007"
     CDS             complement(6086..7558)
                     /gene="der"
                     /locus_tag="RPM73_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDX6518407.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="RPM73_000008"
     CDS             complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="RPM73_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDX6518408.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="RPM73_000009"
     CDS             complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="RPM73_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDX6518409.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="RPM73_000010"
     CDS             complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="RPM73_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDX6518410.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="RPM73_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="RPM73_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDX6518411.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="RPM73_000012"
     CDS             complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="RPM73_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDX6518412.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13331..14485)
                     /gene="trmG/rlmN"
                     /locus_tag="RPM73_000013"
     CDS             complement(13331..14485)
                     /gene="trmG/rlmN"
                     /locus_tag="RPM73_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDX6518413.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="RPM73_000014"
     CDS             complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="RPM73_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDX6518414.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15215..17527)
                     /gene="pbpC"
                     /locus_tag="RPM73_000015"
     CDS             complement(15215..17527)
                     /gene="pbpC"
                     /locus_tag="RPM73_000015"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDX6518415.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGMPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17528..22489)
                     /gene="yfhM"
                     /locus_tag="RPM73_000016"
     CDS             complement(17528..22489)
                     /gene="yfhM"
                     /locus_tag="RPM73_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDX6518416.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     SLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22696..23541
                     /gene="sseA"
                     /locus_tag="RPM73_000017"
     CDS             22696..23541
                     /gene="sseA"
                     /locus_tag="RPM73_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDX6518417.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24034..24810)
                     /gene="sseB"
                     /locus_tag="RPM73_000018"
     CDS             complement(24034..24810)
                     /gene="sseB"
                     /locus_tag="RPM73_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDX6518418.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24952..26235)
                     /gene="pepB"
                     /locus_tag="RPM73_000019"
     CDS             complement(24952..26235)
                     /gene="pepB"
                     /locus_tag="RPM73_000019"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDX6518419.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26413..26613)
                     /gene="iscX"
                     /locus_tag="RPM73_000020"
     CDS             complement(26413..26613)
                     /gene="iscX"
                     /locus_tag="RPM73_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDX6518420.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26625..26960)
                     /gene="fdx"
                     /locus_tag="RPM73_000021"
     CDS             complement(26625..26960)
                     /gene="fdx"
                     /locus_tag="RPM73_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDX6518421.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26962..28812)
                     /gene="hscA"
                     /locus_tag="RPM73_000022"
     CDS             complement(26962..28812)
                     /gene="hscA"
                     /locus_tag="RPM73_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDX6518422.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28829..29344)
                     /gene="hscB"
                     /locus_tag="RPM73_000023"
     CDS             complement(28829..29344)
                     /gene="hscB"
                     /locus_tag="RPM73_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDX6518423.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29440..29763)
                     /gene="iscA"
                     /locus_tag="RPM73_000024"
     CDS             complement(29440..29763)
                     /gene="iscA"
                     /locus_tag="RPM73_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDX6518424.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29780..30166)
                     /gene="iscU"
                     /locus_tag="RPM73_000025"
     CDS             complement(29780..30166)
                     /gene="iscU"
                     /locus_tag="RPM73_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDX6518425.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30194..31408)
                     /gene="iscS"
                     /locus_tag="RPM73_000026"
     CDS             complement(30194..31408)
                     /gene="iscS"
                     /locus_tag="RPM73_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDX6518426.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31520..32008)
                     /gene="iscR"
                     /locus_tag="RPM73_000027"
     CDS             complement(31520..32008)
                     /gene="iscR"
                     /locus_tag="RPM73_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDX6518427.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32309..33046)
                     /gene="trmJ"
                     /locus_tag="RPM73_000028"
     CDS             complement(32309..33046)
                     /gene="trmJ"
                     /locus_tag="RPM73_000028"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDX6518428.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33165..33968
                     /gene="suhB"
                     /locus_tag="RPM73_000029"
     CDS             33165..33968
                     /gene="suhB"
                     /locus_tag="RPM73_000029"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDX6518429.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34113..34967
                     /locus_tag="RPM73_000030"
     CDS             34113..34967
                     /locus_tag="RPM73_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX6518430.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35158..36438
                     /gene="csiE"
                     /locus_tag="RPM73_000031"
     CDS             35158..36438
                     /gene="csiE"
                     /locus_tag="RPM73_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDX6518431.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36430..37569)
                     /locus_tag="RPM73_000032"
     CDS             complement(36430..37569)
                     /locus_tag="RPM73_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDX6518432.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37729..38619)
                     /gene="hcaR"
                     /locus_tag="RPM73_000033"
     CDS             complement(37729..38619)
                     /gene="hcaR"
                     /locus_tag="RPM73_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDX6518433.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            38755..40116
                     /locus_tag="RPM73_000034"
     CDS             38755..40116
                     /locus_tag="RPM73_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDX6518434.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40113..40631
                     /locus_tag="RPM73_000035"
     CDS             40113..40631
                     /locus_tag="RPM73_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDX6518435.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40631..40951
                     /locus_tag="RPM73_000036"
     CDS             40631..40951
                     /locus_tag="RPM73_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDX6518436.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40948..41760
                     /gene="hcaB"
                     /locus_tag="RPM73_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40948..41760
                     /gene="hcaB"
                     /locus_tag="RPM73_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDX6518437.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41770..42972
                     /locus_tag="RPM73_000038"
     CDS             41770..42972
                     /locus_tag="RPM73_000038"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDX6518438.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43069..43491
                     /locus_tag="RPM73_000039"
     CDS             43069..43491
                     /locus_tag="RPM73_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDX6518439.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43539..44411)
                     /locus_tag="RPM73_000040"
     CDS             complement(43539..44411)
                     /locus_tag="RPM73_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDX6518440.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44423..45517)
                     /locus_tag="RPM73_000041"
     CDS             complement(44423..45517)
                     /locus_tag="RPM73_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDX6518441.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45550..46548)
                     /locus_tag="RPM73_000042"
     CDS             complement(45550..46548)
                     /locus_tag="RPM73_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6518442.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46573..48084)
                     /locus_tag="RPM73_000043"
     CDS             complement(46573..48084)
                     /locus_tag="RPM73_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX6518443.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48107..49090)
                     /locus_tag="RPM73_000044"
     CDS             complement(48107..49090)
                     /locus_tag="RPM73_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDX6518444.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49187..52468)
                     /locus_tag="RPM73_000045"
     CDS             complement(49187..52468)
                     /locus_tag="RPM73_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDX6518445.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIELQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52586..53779
                     /locus_tag="RPM73_000046"
     CDS             52586..53779
                     /locus_tag="RPM73_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDX6518446.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53843..55096)
                     /gene="glyA"
                     /locus_tag="RPM73_000047"
     CDS             complement(53843..55096)
                     /gene="glyA"
                     /locus_tag="RPM73_000047"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDX6518447.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55424..56614
                     /gene="hmpA"
                     /locus_tag="RPM73_000048"
     CDS             55424..56614
                     /gene="hmpA"
                     /locus_tag="RPM73_000048"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDX6518448.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56659..56997)
                     /gene="glnB"
                     /locus_tag="RPM73_000049"
     CDS             complement(56659..56997)
                     /gene="glnB"
                     /locus_tag="RPM73_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDX6518449.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57058..58392)
                     /gene="glrR"
                     /locus_tag="RPM73_000050"
     CDS             complement(57058..58392)
                     /gene="glrR"
                     /locus_tag="RPM73_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDX6518450.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58382..59095)
                     /gene="qseG"
                     /locus_tag="RPM73_000051"
     CDS             complement(58382..59095)
                     /gene="qseG"
                     /locus_tag="RPM73_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDX6518451.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59260..60687)
                     /gene="qseE"
                     /locus_tag="RPM73_000052"
     CDS             complement(59260..60687)
                     /gene="qseE"
                     /locus_tag="RPM73_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDX6518452.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT        14410 a        16866 c        15387 g        14401 t
ORIGIN      
        1 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
       61 cagtcaaatg ctgacgatca tgctgccgat gtttatcaaa ccgatcaact gaataccaaa
      121 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
      181 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
      241 gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg tgcagttggc
      301 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
      361 gtttctttaa cctataagga aggcaccaaa gtgtatacct ctacccaggt gggtaaagag
      421 tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga aacgcgtatt
      481 cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
      541 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
      601 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
      661 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
      721 gcccaggccg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
      781 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
      841 ctacgcagct tagagattca gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt
      901 aagcgcgaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
      961 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
     1021 agcgtgggca aagcagaaga gaacaaatcg gaaagctggt ttaattaatc gatgttagta
     1081 acttcaagcc tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
     1141 attcatagtg cagggtcaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1201 tcatacacca cgcgagaaat accgttcact tcattgataa tgcggttgga aacacgaccg
     1261 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1321 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1381 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1441 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1501 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1561 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1621 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1681 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1741 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaacacttc aacgaaaacg
     1801 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1861 cgatcttctg ccggtacgtg aacaatgttc agaccaaagt gatcgccaaa catatccaga
     1921 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     1981 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2041 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2101 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2161 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2221 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2281 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2341 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2401 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2461 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2521 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2581 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2641 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2701 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2761 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2821 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2881 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2941 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3001 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3061 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3121 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3181 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3241 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3301 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3361 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3421 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3481 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3541 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3601 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3661 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3721 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3781 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3841 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3901 taacgaccgg atagcccgca aaaccgttac gctcagtcag ttctttcact tcgcgcagcg
     3961 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4021 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4081 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4141 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4201 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4261 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4321 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4381 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4441 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4501 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4561 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4621 ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc atggacaaca agttttagtt
     4681 cgcgccaata ttacgctcta cgagccgcgc ggtgactacc agataatcgt tgagagtatg
     4741 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4801 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4861 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4921 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcaccg
     4981 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5041 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5101 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5161 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5221 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5281 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5341 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5401 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5461 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5521 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5581 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5641 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5701 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5761 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5821 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5881 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     5941 tccccgcagg atgggcaacg cgcatggtcg ttatcctgat caagcacatg ctgacattgt
     6001 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6061 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6121 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6181 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6241 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattacca tgaatcacca
     6301 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6361 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6421 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6481 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6541 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6601 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6661 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcatct tcaatggcct
     6721 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6781 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6841 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6901 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     6961 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7021 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7081 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7141 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7201 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7261 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7321 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7381 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7441 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7501 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7561 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7621 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7681 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7741 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7801 cgacgttaat ccagtgcaga taaccttcac tgtcacctac caccaggttg ccattataca
     7861 gcaccggaga agtcagcagg cgatgcaaca gatcgctttg tgtccacagc gtaacgccgc
     7921 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     7981 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8041 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8101 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8161 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8221 cgccacccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8281 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcctcg ttcagcgctt
     8341 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8401 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8461 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc agaccgccag
     8521 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8581 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8641 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8701 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8761 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8821 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8881 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     8941 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9001 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9061 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9121 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9181 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9241 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9301 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9361 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9421 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9481 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9541 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9601 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9661 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9721 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9781 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9841 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9901 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
     9961 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10021 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10081 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10141 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10201 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10261 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10321 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10381 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10441 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10501 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10561 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10621 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10681 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10741 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10801 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta tgcgggttca atcatacacg
    10861 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10921 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    10981 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11041 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11101 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11161 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatcccgcg
    11221 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11281 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11341 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11401 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11461 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11521 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11581 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11641 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11701 gacgccgtat tccgctactt tcagtgcaat gcgatagtcg aagtggatgt cagccaccag
    11761 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11821 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11881 ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    11941 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12001 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12061 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12121 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12181 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12241 tcagtaacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12301 gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt
    12361 gttgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12421 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12481 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12541 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12601 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12661 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12721 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatcg gcgcaacttt cgcagcccgt
    12781 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12841 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12901 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    12961 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13021 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13081 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13141 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13201 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13261 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    13321 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13381 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13441 caccacgggt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13501 ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc cgggaagggg ttccacggga
    13561 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13621 tgccgtcgtt aacgtggtca agcatcacat actcaatagt gacacggccc tgattggcgt
    13681 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13741 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13801 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13861 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13921 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    13981 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14041 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14101 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14161 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14221 atgaacgctg ttcttcaacc acttccggtg cgcggatctc cgccacctct ttcagtttgc
    14281 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14341 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14401 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14461 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14521 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14581 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14641 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14701 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14761 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgcac
    14821 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14881 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    14941 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15001 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15061 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15121 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15181 aatgagtttt agcaacaatc aggggcaaaa cagactattg catgacaaat ttcactgtcg
    15241 cgatttgccc cacatcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15301 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15361 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15421 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag cttggcatcg tgaccgtatg
    15481 gcgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15541 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15601 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15661 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15721 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15781 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15841 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15901 gcgtgccgtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    15961 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16021 gcaaggcact atccggcaag ggtttcgctt catcagccat aatccggcga atgatccacg
    16081 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16141 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16201 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    16261 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16321 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16381 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16441 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16501 ccgatcctgg cgatcggatc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16561 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16621 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16681 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16741 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16801 cttcccttga ctcttttacc tgctcacggg accacacacc ttgcacggcc atccgttcga
    16861 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16921 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    16981 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17041 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17101 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17161 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17221 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    17281 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17341 agatgccgtc agcatcggcg aagcgccata gcggcatacc atcctgcgcc acgaccactc
    17401 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17461 gaaaaaggag aaatggcgca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    17521 gaggcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    17581 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17641 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17701 gatcgtcacg gaactcaatg tgcttaatgc tcgcctgctg catctggttc agtaagtttt
    17761 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tgattttcca
    17821 gttccagacc cgcaggcagc agatccacga ctaacgcatc cggcacgctg ttactggctt
    17881 ttacctgcaa ccagaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    17941 taccatcagt accaagaata tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg
    18001 cggattgcgg ataaccgctg gcatccatac gcaaccataa cggctgatcg ccactgtttc
    18061 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    18121 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18181 tacgggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg
    18241 cctgctgcga aagtgtgttc agcaaagtgt actgctcatc gggtagcagt ttattttctt
    18301 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18361 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    18421 ttgcatcacc catggttttc agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg
    18481 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    18541 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18601 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18661 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18721 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18781 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18841 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18901 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    18961 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19021 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19081 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19141 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    19201 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19261 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19321 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19381 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19441 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    19501 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19561 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19621 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19681 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19741 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19801 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19861 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19921 ggttttcatc gccaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    19981 gagatttatc gccaggacgt accaccagcg tacttaaata cagatcatga cgattccagg
    20041 ttttatcgac cggaatcgtc agatccagcc cttgagcccg aacatcaatc tcttgccacc
    20101 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcga
    20161 tatgcaactt aatggtgtcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    20221 ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga
    20281 aacgaacact actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20341 ctaccggaaa actgaccttg ccggtttcgt ccgctttcag atccagagtt tgttcatttt
    20401 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20461 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20521 cttgcgcatc gctataaacg atgtcaaaag cggcgttact gccttcatca acaatgggct
    20581 gtttcaccgt gctgtcagta cgataatcgt aaaccgattt cgaggcgaac tgcggacgga
    20641 tccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20701 cgcccgattc cagcaaacta ccctggaaaa taacctgtaa tggggaatgc gtttccttcc
    20761 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20821 ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20881 acacagcttc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    20941 gtgcaccata caggtagtac cccaccacgg agaatttcac ttcatctttc ggcgttagcg
    21001 gggttttctc accggtcaga ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga
    21061 aatcccacat ccgatactgg ttatcgcccg tgttagcgcg aatatgccac ataccggttg
    21121 ccgcattgct atcgagtgac caggtaaagt ggtagaggcc attctccggc tgactaacga
    21181 cgctcctgag tacctgccca tcgggtttaa tcacgtctaa cttgatgggt tgattgggca
    21241 acgctttacc gtctgcatca cgcagcaaac cattgaggat taccgtttca cctgggcgat
    21301 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21361 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21421 tacgcgccaa cagtaatgcc gcgtttttat cattttccag ctgcacatgc ccctgagcgt
    21481 cactggttgc ctgagtcaga gtctgccctt tctcatttaa taaagagact tcaattcctt
    21541 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21601 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21661 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21721 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21781 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21841 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21901 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    21961 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22021 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22081 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca
    22141 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22201 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22261 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22321 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22381 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22441 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22501 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22561 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22621 tcagcctctt tcgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22681 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22741 attgatgacc cggaaattca gattatcgat gcccgcatgg cgtcgcctgg acaggaagat
    22801 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22861 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22921 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    22981 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23041 gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg atgatctgct gttagaagaa
    23101 ggtgcagtag agctgccgga aggagagttt aacgccgcgt ttaatcctga agccgtggtg
    23161 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc
    23221 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23281 attcccggag cactgaatgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23341 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23401 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat
    23461 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta
    23521 ccggttgagc cagtgaaata agtattttac aggcaataaa aaaccgccga atttggcggt
    23581 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23641 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23701 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23761 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta
    23821 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23881 agatacggtg cttctgtatc gtacttattg tttctggtgc gctgttttcc caagtaaata
    23941 ataaccggag tctctccggc gacaatttac tggtggttaa caaccttcag agcagcaagt
    24001 aagcccgaat gccgcccttt ggggcggcat attttagatt atccgattct gtttaaagtc
    24061 acgcaaaaaa ccaccccagc gacgttcata gaatggcaca atatgttcgg taataaagtg
    24121 gctaattcct ttttcccctt ttttcacctg acaaatatcg attggttcat cgccaggtaa
    24181 tgtatcggtc gctacacttc ccgtcgcctg aataatttct tcgatatcac catcggcttc
    24241 aatgccaata agtaaattag gctgtgcctc ttcgttctct ttaattgaac aaataaaagc
    24301 acgcttcacc gacttaatgg ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg
    24361 cggctctgcg acttccgata atatcagcga ttcaccgcct tccaggattt cctggctgct
    24421 cagcggattt ccctcttcac caatcaacaa actgatttca cgcggcataa attctttacc
    24481 ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg
    24541 aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc tgttgaagcg cttctaacga
    24601 ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc
    24661 actatcttca accacagcct cgccctgcgc cgccgtacca ggcacccaga cggtggattc
    24721 cagtagagta cggaaaaagg ccgggcggtg cgccggttcg gttgctgctt tttccagcag
    24781 gtcttcaagt tcgttttttg tttcggacat atgaaccaca attcattcaa cgttcggggc
    24841 gcaaatgcct gatgcgctac gcttatcagg cctacaaggg attcgcaatt tgttgaattt
    24901 gcagaatttg taggccggat aaggcgttta cgccgcatcc ggcatattag tttacgccgt
    24961 taacagatta gctatcgtgc gcacaccaag tcccgtagcg cccgcagacc actgttcaac
    25021 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
    25081 aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc
    25141 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    25201 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    25261 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    25321 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    25381 ggcaagcacc agacgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    25441 ataggtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    25501 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    25561 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    25621 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acaccggtgc
    25681 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    25741 gcgcccgacg gtgtgcagcc ccatataacc ttgctcacgc agatcttcgc ctttggtgat
    25801 ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    25861 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    25921 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    25981 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca
    26041 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    26101 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    26161 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    26221 catcgcttct gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca
    26281 ggatttttcc gctaaatgat tcgcgttgca ggaaggcggc aagtgagtga agccccagga
    26341 gcatagataa ctatgtgact ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc
    26401 atgacgcgga aattattcgg cctcgtccag ccagactaac aaaatcgctt cgaggatttt
    26461 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
    26521 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
    26581 aatttcgcgg ctatcggtcc atttaagtcc catactaacc tctgttaatg ctcacgcgca
    26641 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
    26701 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
    26761 tctgagcttt ccggcagcga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
    26821 gcacaggatt tttcacaggc gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga
    26881 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagagatc ctgatgaggc
    26941 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
    27001 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
    27061 cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc
    27121 atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
    27181 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca ttcgggcttt
    27241 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
    27301 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
    27361 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
    27421 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
    27481 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
    27541 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
    27601 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
    27661 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
    27721 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
    27781 caccagcggc acgcgagtag aaccgccaac catcaccact tccagcactt catcagcttc
    27841 tacgcccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
    27901 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
    27961 cacggagtcc gcatcgctca gggcgatttt ggccgcaatg gcggcatcca gcagttcacg
    28021 ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgt tcgcgaatgt aatccgccag
    28081 tagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
    28141 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
    28201 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cggtcggttc
    28261 gttaagtaag cgcaggacgt gaaggcccgc cagacgcgcc gcgtctttgg tgccctgacg
    28321 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
    28381 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
    28441 cagcagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
    28501 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
    28561 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
    28621 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
    28681 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
    28741 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
    28801 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
    28861 cggcactgct ccgcagttta tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt
    28921 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
    28981 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
    29041 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
    29101 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
    29161 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
    29221 cctggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
    29281 ggctcagcgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
    29341 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
    29401 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
    29461 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
    29521 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
    29581 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
    29641 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
    29701 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
    29761 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
    29821 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
    29881 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
    29941 attcggtgac cagggagctg gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg
    30001 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcatcacg tcgccacagg
    30061 ccggtgcccc caccatgccg ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt
    30121 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
    30181 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
    30241 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
    30301 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
    30361 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
    30421 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
    30481 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
    30541 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
    30601 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcctcg cccattccga
    30661 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
    30721 tttgcgcttc gatgcgtacg cgcggtttac gacgtacata cagcgcaccg atacctttcg
    30781 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
    30841 taggcagttt acccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
    30901 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
    30961 tggaaacaag gatggtgtcg tcacgcatcg ctgcttcaag ttctttcagg tcgataatgc
    31021 cgttacgctg cggtgccagg taggtgactt caaaaccttc gcgctccagc tgacggcagg
    31081 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
    31141 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
    31201 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
    31261 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
    31321 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
    31381 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
    31441 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
    31501 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
    31561 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
    31621 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
    31681 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
    31741 tggcatctac agattcgtca acggcgctaa ttacttcgcc aacggcgatg ctgctggcat
    31801 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
    31861 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
    31921 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
    31981 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
    32041 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
    32101 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
    32161 ttttatcgtg gcgtttgtac ctgttgtcct tccagaccat gggcgggttg agggcaatta
    32221 ttcgcattag cgctggcctc ttcccctcac cctaaccctc tccccgaagg ggcgagggga
    32281 ccgtccgtgc atttatctgg cttagccctc aggccttatt acctttattc tgctgctcaa
    32341 tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc gcacgggtaa
    32401 acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata aaaccggttg
    32461 ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc agcggatacg
    32521 gcgtctcttc atgctcgacc tgctcgccgt tttcctgagt tgccagccag gccatgcgca
    32581 cttcataggc aataacctga accgccatcg ccaggttcag cgagctgtat tccgggttag
    32641 ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag ccgacgcgct
    32701 cacgaccaaa caccagcgcc acaggggtat ttgccgcttc agcgacgctt ttcagaccgc
    32761 attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca
    32821 ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca
    32881 catcgctggc ccctgctgcc agggcaatcg cctgggagtc gggtttcacc agtggattaa
    32941 ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa cccatattgc
    33001 cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc tttcttcatc
    33061 taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc gctgctatac
    33121 tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat ccgatgctga
    33181 acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac tatgaaaccc
    33241 cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac gtagataaag
    33301 ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac accatcatca
    33361 ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt atcgatccac
    33421 tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct atcgccgttc
    33481 gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac gaactgttca
    33541 ccgccactcg cggtcagggc gcacagctga acggctaccg tctgcgcggt agcaccgctc
    33601 gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca
    33661 ctacctacat caacatcgtc ggcaaactgt tcaacgaatg tgcagacttc cgtcgtaccg
    33721 gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa
    33781 tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca
    33841 tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc gttgctggta
    33901 acccacgtgt tgtgaaagcc atgctggcga acatgcgtga cgagttaagc gacgctctga
    33961 agcgttaatg actcaggcgg gtgatgtcac tcacccgccc tcgcctttca ggcgctattc
    34021 tgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct ttccccgatt
    34081 aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac aaacgcgttc
    34141 ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg
    34201 ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc tggtcggcgg
    34261 aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt attccttctt
    34321 cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc aatgccggaa
    34381 atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc aacgttttta
    34441 tgtttgatta tcgcgggttt ggtaaatcaa aaggcacgcc gtcccaagcc ggattgctgg
    34501 acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac ccacaacggc
    34561 tggtgctgtt cgggcagagt attggcgggg cgaatattct ggatgttatt ggtcggggtg
    34621 atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat gcaaccatcg
    34681 ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc ggcgaaaatt
    34741 atatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagct gatcacgtta
    34801 tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa cggttgatcc
    34861 taatcccgga tggcgaacac attgatgctt tttccgatcg ccacggcgat gtttatcgcg
    34921 aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc atgatcgcta
    34981 gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc agaacagttt
    35041 aagatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat tcccttcgtc
    35101 atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga tctacagatg
    35161 atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg ccagatcttg
    35221 ctgacgctct ttcagccggg gttgaccgcc accacggcaa ctttcagcga gcttaatggt
    35281 gtggatgatg atattgccag tcttgatatc agcgaaacag gacaggagat cctgcgctat
    35341 catcaactca cactgacggc tggttatgac ggtagctacc gggttgaagg tacagtgctt
    35401 aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg tccatcattt
    35461 attaccagcc atttcacccc cgccctgaag agtgaactga agcggcgcgg aattgcgcgt
    35521 aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg acggctgcaa
    35581 aaacgctttg aaacgcgcga tattcatttc ctgtgtctgt atctgcaata ttgtttgctg
    35641 caacaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc
    35701 tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag
    35761 cctgtaccac ctgatgagcc actgtttatg gcactacttt tttccatgtt gcgggttccc
    35821 gatccactgc gggatgcgca tcagcgagac agacaattgc gtcagtctat caaacgtctg
    35881 gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg gttatgtgat
    35941 cagctttata cccacctcgc ccaggcgtta aatcgcagtt tgtttgccat cggtattgat
    36001 aataccctgc cggaagagtt cgccagactg tacccacgcc tggtgcgcac cacccgcgcg
    36061 gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga aagtggtctg
    36121 gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga aaaacagatt
    36181 attcttctta ccgggaatga tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa
    36241 ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt gcagacaggc
    36301 gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccattacc gctcttttca
    36361 ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca gatccgcaaa
    36421 atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg gaagcgccac
    36481 cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac cggcgaaaac
    36541 ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct gtaaacggat
    36601 gacttcgcta ccctggcgag cagcaatata acgcatggcg gccaggtggc agaccgtgaa
    36661 ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag ttgctcccat
    36721 aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc gtgcactaca
    36781 acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca cgcccagcga
    36841 ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa tggcgctaaa
    36901 accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa agcgccagtt
    36961 ctggcgaacc agcgccaacc acgctgacca accggtgctc tcctgctggc ggcttgcccc
    37021 ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca cgcccaacgt
    37081 caacagcgcg aggatcaccc gataatcaaa catagtgacc agtttgcccg tcagcgccga
    37141 gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa gcgggaactg
    37201 cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga aaaagaggtt
    37261 aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc
    37321 aaagagaagt gtcagcagtg ccagcacgcg caaggcggaa atcaggcggg aaggatcgct
    37381 gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg cccccaataa
    37441 caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc agaaaggtag
    37501 aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc gcgtggattg
    37561 caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga atgttggtgc
    37621 aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag
    37681 cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac
    37741 gtttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccggct tcatctcccc
    37801 tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg gacgaaaaac
    37861 aacctgtccg gtattaaaat tattcatata accgggtatc aaagtgacgc ccagccccat
    37921 gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat ttggctggct
    37981 gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat acgccggatc
    38041 ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt catgtgctaa
    38101 agggtgatca accggtaaca caaccactaa tggttcgtca aaaagctcca gataatcaat
    38161 ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt
    38221 ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg gctgtctgag
    38281 acgaaacatc ggtaatactt ttggcagtaa attcacttcc gccgatggca caaagccaat
    38341 ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat tttccgcttg
    38401 ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca acgcgacttt
    38461 gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc ggatctggct
    38521 gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg
    38581 cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct tgttatcgaa
    38641 aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac cagcaacatc
    38701 cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa aaatatgacc
    38761 acaccctcag atttgaacat ttaccaactg attgataccc aaaatggtcg ggtcactccg
    38821 cgtatttata ccgacccaga tatttaccaa ctggagcttg aacgtatttt cggccgttgc
    38881 tggctatttc tcgcccacga aagccagatc ccaaaacctg gtgatttctt taacacctac
    38941 atgggggaag atgcggttgt cgtagtgcgt cagaaagacg gcagcatcaa ggcgtttctc
    39001 aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgcggcaa cactcgcgcc
    39061 tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat cgatgtaccg
    39121 ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actggggact aaacgaagtt
    39181 ccttgtgtgg agagttataa agggctgatt tttggcaact gggataccag cgcaccgggc
    39241 ctgcgtgatt acctgggtga cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa
    39301 ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc
    39361 ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc ttctgccgtt
    39421 caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc
    39481 cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc
    39541 ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc agtttcaagc
    39601 tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc
    39661 ctggcgggtc ataacaatat tttccccacg ctttcatggc taaacggcac tgccacactc
    39721 cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg tattactgac
    39781 aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg tgcttttggt
    39841 cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca gaaattgctt
    39901 aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag
    39961 cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac tgccgctcgt
    40021 ggaatgtacc aacgctgggc tgatcttctg agtagcgaaa gctggcagga agtgctcgat
    40081 aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca ctagagttac
    40141 atcaccgcat tagccagttt ctctttcacg aagccagctt actggacgac tggaaatttc
    40201 gtgactggct ggcgcagctc gacgaagaga ttcgttacac catgcgcacc acagttaacg
    40261 cgcaaacacg cgaccgccgc aaaggcgtcc agccaccgac aacctggatt tttaatgaca
    40321 ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc tgggcagaag
    40381 agccgccgtc acgcacccgt cacttaatca gcaactgcca gataagcgaa accgacatcc
    40441 caaacgtatt tgctgtgcgg gtaaattatc tgctttatcg ggcacaaaaa gagcgcgatg
    40501 aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat gacaactggc
    40561 gcttgctgga acgggatatc gtcctggatc aagcggtaat cacttcccat aacctgagtg
    40621 tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc
    40681 tctccggatc gatacctcgc ccgtcatcgc cctgttcaac gttggcggcg agttttatgc
    40741 cattaacgat cgttgcagcc atggtaatgc gtcaatgtca gaagggtatc tggaagatga
    40801 cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt
    40861 atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag gtggtgacat
    40921 tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc catttttatt
    40981 accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga agaaggcgcg
    41041 caggttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc
    41101 gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca acgcgcggtc
    41161 gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc aggcatctgg
    41221 gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg cttccacgag
    41281 ctgtttaacg tcaatgttct cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta
    41341 atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta tcctggcggc
    41401 ggtggcccgc tgtacaccgc cagtaaacat gccgcaaccg gacttattcg ccaactggct
    41461 tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac
    41521 ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc tctgacgccg
    41581 gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga ttttacgggt
    41641 ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac
    41701 gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct ggatctttaa
    41761 ggaagcacga tgaaagaaaa aaccatcatt attgtcggtg gcgggcaagc ggcggcaatg
    41821 gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt ttccgatgag
    41881 cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga agattccccg
    41941 cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca tctgcattcc
    42001 ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac caacggcgaa
    42061 agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc gctgccgttg
    42121 cttgatgcac tgggagaacg ctgctttacc ctacgccatg ccggtgatgc cgccagactg
    42181 cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac tattggtctg
    42241 gaactggctg ccagcgccac gcagcgcaga tgtaaggtga cagtgattga actggcggca
    42301 accgtcatgg gccgtaatgc accaccgccc gtgcaacgct atcttttaca gcgccaccag
    42361 caggctggtg tgcgcattct gctcaataat gccattgaac atgtggtcga tggtgaaaaa
    42421 gtagaactga cgctgcaaag tggggaaacg cttcaggctg atgtggtgat ttacggtatt
    42481 ggtatcagcg ccaacgagca actggctcgc gaggccaacc ttgatactgc caatggcatt
    42541 gtcattgatg aggcttgccg cacctgcgat cccgcgatct ttgccggtgg cgatgtggca
    42601 atcactcgtc ttgataatgg tgcactacac cgctgcgaaa gctgggaaaa cgccaataac
    42661 caggcgcaaa ttgccgctgc cgcaatgtta gggctaccgc taccgctact gccgccgccg
    42721 tggttctgga gcgatcagta cagtgataac ttacagttta ttggcgatat gcgtggcgat
    42781 gactggcttt gtcgtggcaa cccggaaact cagaaggcga tttggtttaa tctgcaaaac
    42841 ggcgtgctta tcggtgcggt cacgctgaat caggggcgtg agattcgccc aattcgcaaa
    42901 tggatccaga gcggcaaaac gtttgatgcg aaactgctga tagatgagaa catcgcgctt
    42961 aaatcactgt aaccaggata attagcgaat atctcaatgc ctggggcgtg gcgaggtgca
    43021 agagtgtgta ttacgtttaa atcacattat cttgcaaagg gattggttat gaacacacta
    43081 cgttattttg attttggagc tgcccgcccc gttttgttat taattgcccg tatcgccgtg
    43141 gtcttaattt tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa
    43201 tatatggcct cgttgggcgc gccgatgccg atgctggcag cgattattgc ggtagttatg
    43261 gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca cccgtccgct ggcggtgctg
    43321 tttattttct acacgctggg tacggcggtg attggtcacc attactggga tatgaccggc
    43381 gatgcggttg ggccaaatat gattaatttc tggaagaatg tcagtatcgc tggcgcattc
    43441 ctgctactgg caattaccgg gccgggggca atttctctcg atcggcgtta ggaaagatgc
    43501 cggatgcggc gtgaacgcct tatccggcat ttaataaatt acagccactc tacccgcaac
    43561 gacatctctg aagttgttga ttcccctggt gctagcgcaa tgagatcacc cccttccggg
    43621 cgatgatgat catccggcgc atggctcatc ggttccagac agaagaaatc aaacgcatat
    43681 cctttatcaa acgcagggtc ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt
    43741 tccatgatga tggcataacc ttcctgcggc tgctcgatgc gggcctgacc attccatccg
    43801 gcaaaaccat tgttcaccca ctggcgcggc aacggcgcgg gctggttaaa atccagttcc
    43861 tgcggtagct gctcgcaaaa ctcacccgcc agccactgct cccgctccag ccagtaaccg
    43921 ctcgcctgtg cctgaatccg cgtttgcggc gacaacggaa aataaggatg ccagccggta
    43981 ccaaatggca gcgtctctgc cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc
    44041 gccgttaaat gaaacgcctg acttacccga tagtgataga caccgctgcg atgttcatac
    44101 accagacaca aactatcatc gctgtgcgag acacattgcc actcgcccag ccagccatcg
    44161 ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca gttgatactc acgcccctgc
    44221 cagacaaaac gattgccgct cacccggttt gcaaatggca ccagcggaaa acatgaggca
    44281 tcggttgcca caccgctttt tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag
    44341 ccttcgatta cgccgccctg gtcagaaacg tccagcttta gcgacccgtg ggataaggta
    44401 tagatggtca tccgctaact ccttaatccg ggaagttaat cacaactttc ccgcatttgc
    44461 cgctcgccat cagcgcatag gcatcacctg cctgttccag cgagaagcga tgggtaatgg
    44521 cgttacgcgg ccacagcttc cagtccgtca gatcatgggc gcatttttcc atatggaaca
    44581 gactggtcac ccaggagccg ataatccgcc gttgatggtg catcagatcg gcgctgacct
    44641 cgaattccac ttttccggtt tcaccaatgt aaaccacccg tccccagtca gcggtggatt
    44701 gcagtgccag caagcgacct gcggcattac cggaacaatc gagcgcaaca tccgcgccac
    44761 cgtgggtgag ttcggcgata atctgcggca gaccttcggt ggttgctaaa tagccgtgat
    44821 ccatcacccc taactgtttt gccatcgcca gacgttccgg cagcatatca acgccgatga
    44881 tccgttttgc accgcgacct ttcgccagca tcatcgccat catgccgacc ggccccagac
    44941 cgaccaccag cacgttatcg ctgccggaaa cttcgccgcg caaaatccct tcataggccg
    45001 tgccaacgcc gcaactgata aacgcaccat cttcgtagct cagcgcatcc ggcaggagga
    45061 tcagatcttt ttcttccgcc agcagatatt cggcatgacc gccgtcacgc tgccagccgt
    45121 aagccgcttt tcctttgcca gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa
    45181 aaccacagcc agaaatgtga tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc
    45241 cttgccccat cgccacaatc tgcccgcacg gttcatgacc gttgataaat ccttggtata
    45301 acggtttgtc gggtgccgct gccgtagcgc ggtgctggtg gtagatatag tggacatcgc
    45361 ttccgcaaat tccggaagat ttcattttga tcagtacctg gttaatccct ggtgtcggca
    45421 ccgcaacttc ccgtagatcg acggtcgaat ttcctggtaa ataagctgcc agcatcgttt
    45481 tcataaatcc ctcattaaca atacgattaa ttttcatccc tgccgcaccc gcgccagggg
    45541 cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc agcaagatat tcgccagcac
    45601 cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa aaggagttaa taccgagcag
    45661 caccagaccg ttaccgatta gcgtaatcac cagcacacca agcaatgtac cgaacaggga
    45721 accgcgaccg ccggaaagtg ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc
    45781 cagaccgttt gcggcacctg cgttaccaga accgaggcgc gccgccaaca aaatgccggt
    45841 caccgccgct aataatcccg aaagggtaaa gataagaatg cgcacccgac gaacgttgat
    45901 gccgcacaac tgcgccgccg tagcattacc gccaacggca aaaaccgagc gcccgaaggc
    45961 ggttttgcgg ctgatgaaca caaacagcgc aaacaacaca atcatgatca gcgcggatac
    46021 cggcacaccg agaaattgtc cgcccagcca gtccagcacc tcgttttcgt caatcggcac
    46081 tggcagtgcg ttcgtcataa acagccccat tccgcgcagg gcgctccaca gccccagtgt
    46141 ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc ccggccagcg ttcccatcag
    46201 cgcgcctaac agcaacacca gcagacacgc caccgccagc ggaacttcaa attgcagcaa
    46261 aaatgccagg cacaccgaga caaaagccac catcggccca acgctgacat caatttcacc
    46321 ggagataata atcagcgtca tcgcccaggc ggcaatccca atggtggcgg catcgcgcag
    46381 cacgttcatc tggttattca atgagataaa gccaggcgcg ttcagggaga agaccagata
    46441 aagaatggcg attaccacca gcaaaccgat ctcattaata tggcgactga caaattgttt
    46501 gagcgagacg ctcttgccct gcggcaatga taatgacgaa gcagacatct tcaggttcct
    46561 cggtaaaatt catcagtgca cagacagaat ggcggacatc agctcatcca cattgaccgg
    46621 cgagtgaaac tcctgcgaga acgtaccgtg ctgtaacaac aggatgcggt cgcacaccag
    46681 cggcaactcc tccacttcac tggagataaa caccacgctt tttccttcgg cagccagctc
    46741 acggacaata cggtaaatct gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc
    46801 gagcaacaaa atctggctgg cggcatagac ccaacgaccg atcaccactt tttgctgatt
    46861 gccgccagaa agcgtgccga tgggcgtttc gctactggcg gccttgaccg tcatccgctg
    46921 catcacctct tcggtcaggc ggcggatggt ggaccattgc agtacgccgt tggcgctgat
    46981 tttttgccga ttggtcagca ctgtattttc gtcaacgccc aaccagggaa tgatccccgc
    47041 ttctttgcgg ttttctggcg tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg
    47101 gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt tcatactcct ccagcccaac
    47161 aatcgccttc agcaattcac tgcgccctgc ccccagcagg ccagcaatgc caagcacttc
    47221 gccgcgacgt aacgtaaagc tgatatcctc cagcttgggc ttatggcgta atgcacggac
    47281 ttccagcacg gcctgatcga caatttcctg aggggctacc ggcgcaatat caacgtgatc
    47341 gcgccctagc attagcgaca caatatgatg cgtggaggtg ttttcgagca tcacatcgcc
    47401 cgccacctga ccatcgcgca taacggtggc acaggaggca atgcggcgaa tttcttccat
    47461 ccggtggctg acataaatca ccgccacgcc cagtgccgac atctttttca ccgcgctgat
    47521 caccagttca acttccgcac tggcaagcga actcgtaggt tcatcaagaa tgaccacgcg
    47581 cggctcgccc ttcatcaccc gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt
    47641 tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc
    47701 ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga ggccattgac caaggcagag
    47761 gttttctgcc accgtcagcc cttccaccag acttaattcc tgataaaccg cacgaacccc
    47821 cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat
    47881 ccagatatca ccgctatccg ggcgttcgct gccggtaagc attcgaatga gagtcgattt
    47941 gcccgcgccg tttttaccta acagcgcacg aacttcgcct ttattgagcg tgaagttaac
    48001 gttatccagc gcaacgacgc cggggtaacg cttatttcct gccaccactt ttgctaccgg
    48061 gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag ggcagaccat
    48121 caacgtgcgt tgccagccac tgtttaccat cttccgtttt ggtatagaga tcgattggca
    48181 cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa
    48241 cagcattgcc cattttctta ccggaaatat ccactaccgc tttcagcacc tgattgtttt
    48301 ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa aacagcaatt tttccggcct
    48361 gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc accgcccgac tcccccataa
    48421 tggcgttgag atccggcgtg gagataatca gtttttcacc aacggaaatc gctttatcta
    48481 aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggat tttaatactt
    48541 cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa
    48601 ttttcggctg gtcaatttta ttggcaataa aataatcggc ggcagcgtta cccagttttt
    48661 taccaaattc cagtggatcg ccgaccagat acgccgagac atatttatcg acaccctttt
    48721 gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg cgacgaacgg
    48781 tacggctact gccattttca gacactgccg ataaaataat ggcatcgaca ttacgcgcca
    48841 cgagggtatc aacaaaggta ctttctttcg aaatatcacc ctgggcgtta gtttcaatta
    48901 actgtacctg tactgaagaa tcttttgccg catcctgaac gccctgacgc actccagcgt
    48961 aatatccctg ggtatcaagg tatattgcac caatggtcat ttctttatcc gcagccctgg
    49021 caaatagtgc gctacctaat agcgtagcca ttaataatag attacgggtt gttctcattt
    49081 ttttaggcat agtgcctcct gtagggtttt tattaacaac ggcttattct aattattttg
    49141 tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag tgaacgtagt
    49201 taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggcc ggattaatcg
    49261 ccgtcaattc agcgcgagca gattcatatt gcgcaacctc cccgagtccc gcggacgcca
    49321 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgaca
    49381 acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat actggttttc gccatctctt
    49441 gcgcccactg tttcatttca ctaaacagtt gctgtgcgat ttgttgttcg cccagcagtc
    49501 gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa taactgtgga
    49561 tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat
    49621 cgccctgcgc gttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
    49681 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgctgg
    49741 cctgctgcgg ctgtctggca tcaaggtgct gccaggcgcg taataactgg ttgaggataa
    49801 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa
    49861 tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa attgagcaac tctgctgtca
    49921 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
    49981 gtgtggctcc gcttaactta tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg
    50041 gcgcaagctg ataagcatta tcaagataac gcgcggccag ctcataatcg tgttgcttat
    50101 tccacgcatg gatcgctaac ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc
    50161 aacgttgcca taaggtaatg gctttgttgt agctacgctt gttgtagtag aagcaggcca
    50221 gtaaatgacg ggcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
    50281 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccgc
    50341 gttcggcttt cggcaataaa ctcgcctgca ggtaaagcgg cagcgtgcgc tggcagtcca
    50401 gtgcgttcag catctctgcc gccagggtgg gcattcccca gttaatcagt tgcccggcgg
    50461 tcatcagagc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agggctgatt
    50521 cgctacgacc atcgaaccag ttcagccacc acagagtggc gttcagcgga taatcacgca
    50581 gcagtttcgc gcgctgcaaa cgcgcgttgt cctgacgacc acttaacacc agcagcagat
    50641 tatgcaggca aagcacttcc tgattggttg ggcaggcacg aagactttgt tggcaaaaat
    50701 ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg tgccaggcca taatagccac
    50761 cggctttgct gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
    50821 gtccctgacg ttcgtaagca ctggcgcgaa tcaaactggc ctgtccgcac tgcggatttt
    50881 tgttcagcgc atgtgcgcgt ttcagagcct gagtggcata cgccaccgct tgcgggaaat
    50941 cggcgcggtt atattccagc atcgccagcg ccaggttaca gcgataatcc agcggatcca
    51001 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
    51061 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gacagtggcg
    51121 ctttggcgac gtccggcaac ggcaactctt gcggctgatg ttcctgataa ctcagtacaa
    51181 tattgccgtc ggcatcggag agctcaatgg tcagcctttc aggattgata ccgtgcaaca
    51241 cgccctggat ggcggtcgca ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt
    51301 tgccgatttc gcggatcgcc aggcggtatc cgttcaacgg agagatggca tacagccccc
    51361 actcaatccc ccgctcacta cgttggagtt ctatcaccgc atcgcgggag gcattttgca
    51421 ccatgcccaa agaatgataa ggcaggaaat actgctcaaa acgcttctct tcgtaagcat
    51481 caagccaggt aaaatcgggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
    51541 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
    51601 tccactgttt tttacctggc gcgatatggt ggttggcaac gtgcagcaaa ccgccatctt
    51661 catcgtgaca ccacgcgcca acaaaatcgt actgtgattt ttcagccata tatgaggttg
    51721 gaacgggcac atttttatag cgagaaatgt ccactccagc ggagtagtcc actttgtagt
    51781 aagtgccggt ggcgatgggg aaagcggaga cggctcgttt gccgtgatca aacaccgccg
    51841 ttacatccgg cgggaagacg ctctgatgcc cttccccccc tttcactgcc gggttggccc
    51901 accataagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagcg
    51961 ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg taaaccatgc atcggctccg
    52021 tttcgccgac ccacaccgtc tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg
    52081 gcataaaggt ggtcgggcga tggtgttgcg gccagttaaa ctcaatccca ccggagatcc
    52141 acggtcccag cagccccacc agcgcaggtt taatgacttc attgtgatag acaaaatcgc
    52201 gctgtttcac tttatcccat gcgcgatgca cccgaccgcc cagttccggc aggatcatca
    52261 ctttgatgta gtcgttttcc agccacaccg cctgccagga tttcagggtt ttctgctcgc
    52321 tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga cgatccctga taaacgcgat
    52381 tttccaggaa catgggatgt atatcctgcg gcccggtttc ataggtcggg atctcaacgc
    52441 gctcttgcca cacttttact ggagtcatgg tgccctcaat attaacaaga catactgaat
    52501 taaaagattt gtggcagtgt attgaacaat ctggcaatgt tttcgcggaa taatcacgca
    52561 attaactaaa caaggtttag tgaagatgag agcctgcatt aacaaccaac agattcgcca
    52621 ccataacaaa tgcgtgattc tggaactgct gtaccggcaa aagcgcgcca ataaatcaac
    52681 gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt aatattttgc aggaactgga
    52741 aagcgaaaaa cgggtggtga atatcgacga tgaaagccag acgcgcgggc atagtagcgg
    52801 tacatggctg attgcgccgg aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag
    52861 tattgagtgt caggttgcta atgcttgttt aagtccgaaa ggcgaatttg agtatttaca
    52921 gattgatgta ccgacgccgc aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca
    52981 ccgtaaattg tggccggacc gcaccatcaa tctggcgctg gcaatccacg gtcaggttga
    53041 tccagtgaca ggagtgtcgc aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt
    53101 gaagtatctg ctggaagaga agctgggcat tcgggtgatg gtcgataatg actgcgtgat
    53161 gctggcgctg gcggaaaaat ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa
    53221 cgttgattac ggcattggct cgtcgttcgt gattaacgag caaatttatc gcggcagttt
    53281 gtatggtagc ggacagattg gtcacaccat cgttaatccg gatggcgtcg tctgcgactg
    53341 tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagc gcattaaaaa aacaggcgcg
    53401 ggtatggcta aaatcacaac cggttaatac tcagcttgat cctgaaaaat tgactaccgc
    53461 gcagttaatc gctgcctggc agagtggaga accgtggatc accagctggg ttgaccgcag
    53521 tgccaatgcc attggtttga gtctgtataa cttcctcaac atcctcaata ttaatcagat
    53581 ttggttgtac ggtcgcagtt gtgcctttgg tgagaactgg cttaatacta ttattcgcca
    53641 gacgggattt aacccgttcg accgcgacga aggaccgagc gtgaaagcga cgcaaattgg
    53701 cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt ggctatttgt atgttgaggc
    53761 gcagttacga cagatttgat ggcgcgataa cgtagaaagg cttcccgaag gaagcctttt
    53821 ttcaacagat taaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    53881 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    53941 tccagccagc cagttctttc gcttctgctt ctttgaagcc gcgacgcgta attgccggag
    54001 tacccacgcg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    54061 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    54121 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    54181 cgcgttccag gaacacttct accatcgctt tagcgttttt agcgacctgc tgctggtaag
    54241 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    54301 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    54361 cgctaccacc tttcgccagg atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    54421 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    54481 ccgcaacgtg cgccatatca acgaacaggt aagcaccgat gctgtcagcg atttcacgca
    54541 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    54601 tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat atgaccggta gcatcgatac
    54661 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    54721 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    54781 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    54841 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    54901 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    54961 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    55021 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    55081 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    55141 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    55201 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    55261 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    55321 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    55381 catttgagat acatcaatta agatgcaaaa aaaggaagac catatgcttg acgctcaaac
    55441 catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa agttaaccgc
    55501 ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt ttaacatgag
    55561 taaccagcgt aatggcgatc aacgtgaagc actgtttaac gctattgccg cctacgccag
    55621 taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac
    55681 cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt tggcaacgct
    55741 ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag cctatggtgt
    55801 actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc
    55861 cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc gcagcgcgct
    55921 tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat accgtccggg
    55981 gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa ttcgtcagta
    56041 ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg
    56101 tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt
    56161 cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga cgttaatctc
    56221 tgccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa aagcaggcca
    56281 cacagcacaa gtgaactggt tccatgcggc agaaaatggc gatgttcacg cctttgccga
    56341 tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct ggtatcgtca
    56401 gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga tggatttgag
    56461 caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg gcccggttgg
    56521 cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg aaaacattca
    56581 ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat ggaaacatcc
    56641 ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat
    56701 gacccgtgcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg tttgcgccgt
    56761 gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt
    56821 cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg tatggccttt
    56881 ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga cttcggccag
    56941 tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct ttttcatgct
    57001 attccttgaa aaggtcgcct gtcttttgat ctgctaaacg taacacataa cgccaattca
    57061 ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa attctgtccg
    57121 gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtaattt gcagcagctt
    57181 acgcaaatag ttgagttcaa actgattacg tgcctcaaca aaggttggca gcgccgtgtt
    57241 ttcaccctcc agcgcctgct ctactaatgc atcgctaata accggagatg aggtcagcgc
    57301 cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccaac tagcggtcat
    57361 caggcgtttc atcgcatcag tggagaacgc gcggacaaac ggtttatgtc gctctgccgc
    57421 ctggcgcaac aggtgatttg ccagaagcgg aatgtcttct gtgcgctctg ccagagccgg
    57481 aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt ccccgcgcgc
    57541 catcgctttt ggcagatcac ggtgagtggc agaaataatc cgcacgtcga tatcaatatc
    57601 gcggttgctg cccagcgggc gcactttacg ctcctgcaac acgcgcagca gtttgacctg
    57661 taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt ccgccgcctg
    57721 gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag
    57781 ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa atggtttgct
    57841 attgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg
    57901 gccgttaatc aaaacgctaa cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg
    57961 cagcatcagc gggctgcggg tgacaatcgc ctcgcgccag cgttcatcgg tggctggcgc
    58021 ggattgttcc agcgcatcgt caattgcctg atatagcgca tctttatcga caggcttggt
    58081 gaggaaactg aacacgccct gctgtgttgc agcaacggca tcgggaatag aaccatgcgc
    58141 ggtaagaata attactggca ttcccggctg cactttctga atttcagcaa acagctgcat
    58201 accgtccatt tcatccatcc gcaggtcgct gatgactaaa tctacttttt cgcgattcag
    58261 tacccgtaat ccttcagcgc cactttccgc cgtgaccacg ctgtagcctt cgctggtcag
    58321 gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata aatgcgcagg
    58381 tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca ccctcgtgcg
    58441 gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt tcaatatcag
    58501 tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct
    58561 ggcgcaatgt atccagctcg ctgtcgctgg cctgctggag cttgccgtag cgttggcgct
    58621 cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac agagggcgaa
    58681 cctgtgccgg gatctcggta cttagcgcat caatgcgcgc gaccagctgg cggcgctcat
    58741 acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc
    58801 cgtcgtcata ttggcgagct tgctgacgtg actgcgcagg cattaaacga tcagcacaat
    58861 ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc agcgcccaga
    58921 tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt ttttcttctg
    58981 ctggcgtatc aatggctggc ttattgtgac tctgcacgca acccagcaat gccagacatg
    59041 gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt cgcatattca
    59101 ccagacttaa tgcctatccc agtgggcgta attattgcag gcagtttgga catggacagc
    59161 gcggagaaac cggagcgtac acgtcgtacg tgaggatttc gagcactgcc cggggccgaa
    59221 ctggcaaata aaatagcctg atgggatagg ctgttagcat tatttcgtgt ttttcgacga
    59281 cggcaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat acagttcccc
    59341 ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc ccttcaccgc
    59401 cccttttcgc tggtggcttc cttgaaaaaa gggttcgaag atcatggcgc gttcctcttg
    59461 cggaatgggt gtgcctgtat tgatgacatc aatataaacc cgtgcaccat gtaagctgct
    59521 gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt aaagattatc
    59581 cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga gatcgacgtc
    59641 ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaactactg tctccaccag
    59701 cggtgctaac tcaacattct ccagttccac cgcactgtcc gcttgtttac ggttgtaatc
    59761 aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac
    59821 ctctttttgc tctggcgtaa gcggcccgac aacctggtca gccagtaatt cagtgccctc
    59881 gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa attgatggcg
    59941 ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc caaccgagcg
    60001 taactcgctc ggtccactga acgaaacgct attgcccaga gaacgccctt cccccagacg
    60061 gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga aaagtagcac
    60121 catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt gcccacgttc
    60181 ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg tggcctgcac
    60241 catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag catcgggacc
    60301 actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg cctggtaaag
    60361 tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg
    60421 gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac ggtagctacg
    60481 ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt
    60541 gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg cttgccatgc
    60601 cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt gtcgtaatga
    60661 gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga atagcctgat
    60721 gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt tgttatggaa
    60781 taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc tgatatcaga
    60841 agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg
    60901 ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact gtgccaataa
    60961 agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat ggcttcttcg
    61021 ctatttgaat gtaaatcagc cactgtgtcg ccaaaaagag acaa
//
LOCUS       DAPPNJ010000002        57300 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000002 DAPPNJ010000000
VERSION     DAPPNJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.002"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(30..575)
                     /gene="syd"
                     /locus_tag="RPM73_000053"
     CDS             complement(30..575)
                     /gene="syd"
                     /locus_tag="RPM73_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDX6518453.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            643..1491
                     /gene="queF"
                     /locus_tag="RPM73_000054"
     CDS             643..1491
                     /gene="queF"
                     /locus_tag="RPM73_000054"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDX6518454.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1603..2967
                     /gene="ppnN"
                     /locus_tag="RPM73_000055"
                     /gene_synonym="ygdH"
     CDS             1603..2967
                     /gene="ppnN"
                     /locus_tag="RPM73_000055"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDX6518455.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            3524..4813
                     /gene="sdaC"
                     /locus_tag="RPM73_000056"
     CDS             3524..4813
                     /gene="sdaC"
                     /locus_tag="RPM73_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDX6518456.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            4871..6238
                     /gene="sdaB"
                     /locus_tag="RPM73_000057"
     CDS             4871..6238
                     /gene="sdaB"
                     /locus_tag="RPM73_000057"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDX6518457.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            6350..7105
                     /gene="xni"
                     /locus_tag="RPM73_000058"
     CDS             6350..7105
                     /gene="xni"
                     /locus_tag="RPM73_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDX6518458.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(7160..8308)
                     /gene="fucO"
                     /locus_tag="RPM73_000059"
     CDS             complement(7160..8308)
                     /gene="fucO"
                     /locus_tag="RPM73_000059"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDX6518459.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(8336..8983)
                     /gene="fucA"
                     /locus_tag="RPM73_000060"
     CDS             complement(8336..8983)
                     /gene="fucA"
                     /locus_tag="RPM73_000060"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDX6518460.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            9530..10846
                     /gene="fucP"
                     /locus_tag="RPM73_000061"
     CDS             9530..10846
                     /gene="fucP"
                     /locus_tag="RPM73_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDX6518461.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            10879..12654
                     /gene="fucI"
                     /locus_tag="RPM73_000062"
     CDS             10879..12654
                     /gene="fucI"
                     /locus_tag="RPM73_000062"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDX6518462.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQKEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSIMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            12763..14181
                     /gene="fucK"
                     /locus_tag="RPM73_000063"
     CDS             12763..14181
                     /gene="fucK"
                     /locus_tag="RPM73_000063"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDX6518463.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            14183..14605
                     /gene="fucU"
                     /locus_tag="RPM73_000064"
     CDS             14183..14605
                     /gene="fucU"
                     /locus_tag="RPM73_000064"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDX6518464.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            14663..15394
                     /gene="fucR"
                     /locus_tag="RPM73_000065"
     CDS             14663..15394
                     /gene="fucR"
                     /locus_tag="RPM73_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDX6518465.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(15438..16538)
                     /gene="rlmM"
                     /locus_tag="RPM73_000066"
     CDS             complement(15438..16538)
                     /gene="rlmM"
                     /locus_tag="RPM73_000066"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDX6518466.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(16531..16926)
                     /locus_tag="RPM73_000067"
     CDS             complement(16531..16926)
                     /locus_tag="RPM73_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDX6518467.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(16945..17862)
                     /gene="gcvA"
                     /locus_tag="RPM73_000068"
     CDS             complement(16945..17862)
                     /gene="gcvA"
                     /locus_tag="RPM73_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDX6518468.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(18213..18440)
                     /locus_tag="RPM73_000069"
     CDS             complement(18213..18440)
                     /locus_tag="RPM73_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDX6518469.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            18632..19837
                     /gene="csdA"
                     /locus_tag="RPM73_000070"
     CDS             18632..19837
                     /gene="csdA"
                     /locus_tag="RPM73_000070"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDX6518470.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            19837..20280
                     /gene="csdE"
                     /locus_tag="RPM73_000071"
     CDS             19837..20280
                     /gene="csdE"
                     /locus_tag="RPM73_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDX6518471.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(20331..21137)
                     /gene="tcdA"
                     /locus_tag="RPM73_000072"
     CDS             complement(20331..21137)
                     /gene="tcdA"
                     /locus_tag="RPM73_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDX6518472.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(21214..22311)
                     /gene="mltA"
                     /locus_tag="RPM73_000073"
     CDS             complement(21214..22311)
                     /gene="mltA"
                     /locus_tag="RPM73_000073"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDX6518473.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            22520..22596
                     /locus_tag="RPM73_000074"
     tRNA            22520..22596
                     /locus_tag="RPM73_000074"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22554..22556,aa:Met,seq:cat)
     gene            complement(22672..23925)
                     /gene="amiC"
                     /locus_tag="RPM73_000075"
     CDS             complement(22672..23925)
                     /gene="amiC"
                     /locus_tag="RPM73_000075"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDX6518474.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            24157..25488
                     /gene="argA"
                     /locus_tag="RPM73_000076"
     CDS             24157..25488
                     /gene="argA"
                     /locus_tag="RPM73_000076"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDX6518475.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(25550..27376)
                     /gene="recD"
                     /locus_tag="RPM73_000077"
     CDS             complement(25550..27376)
                     /gene="recD"
                     /locus_tag="RPM73_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDX6518476.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHHHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(27376..30918)
                     /gene="recB"
                     /locus_tag="RPM73_000078"
     CDS             complement(27376..30918)
                     /gene="recB"
                     /locus_tag="RPM73_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDX6518477.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(30911..33799)
                     /gene="ptrA"
                     /locus_tag="RPM73_000079"
     CDS             complement(30911..33799)
                     /gene="ptrA"
                     /locus_tag="RPM73_000079"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDX6518478.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(33975..37343)
                     /gene="recC"
                     /locus_tag="RPM73_000080"
     CDS             complement(33975..37343)
                     /gene="recC"
                     /locus_tag="RPM73_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDX6518479.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(37356..37679)
                     /locus_tag="RPM73_000081"
     CDS             complement(37356..37679)
                     /locus_tag="RPM73_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDX6518480.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(37664..38071)
                     /locus_tag="RPM73_000082"
     CDS             complement(37664..38071)
                     /locus_tag="RPM73_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDX6518481.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(38068..38631)
                     /locus_tag="RPM73_000083"
     CDS             complement(38068..38631)
                     /locus_tag="RPM73_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDX6518482.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(38622..39092)
                     /locus_tag="RPM73_000084"
     CDS             complement(38622..39092)
                     /locus_tag="RPM73_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDX6518483.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(39277..40071)
                     /gene="thyA"
                     /locus_tag="RPM73_000085"
     CDS             complement(39277..40071)
                     /gene="thyA"
                     /locus_tag="RPM73_000085"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDX6518484.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(40078..40953)
                     /gene="lgt"
                     /locus_tag="RPM73_000086"
     CDS             complement(40078..40953)
                     /gene="lgt"
                     /locus_tag="RPM73_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDX6518485.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(41104..43350)
                     /gene="ptsP"
                     /locus_tag="RPM73_000087"
     CDS             complement(41104..43350)
                     /gene="ptsP"
                     /locus_tag="RPM73_000087"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX6518486.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(43363..43893)
                     /gene="rppH"
                     /locus_tag="RPM73_000088"
     CDS             complement(43363..43893)
                     /gene="rppH"
                     /locus_tag="RPM73_000088"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDX6518487.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            44578..45267
                     /gene="mutH"
                     /locus_tag="RPM73_000089"
     CDS             44578..45267
                     /gene="mutH"
                     /locus_tag="RPM73_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDX6518488.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            45336..46049
                     /locus_tag="RPM73_000090"
     CDS             45336..46049
                     /locus_tag="RPM73_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDX6518489.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            46187..46405
                     /gene="ygdR"
                     /locus_tag="RPM73_000091"
     CDS             46187..46405
                     /gene="ygdR"
                     /locus_tag="RPM73_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDX6518490.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            46513..47553
                     /locus_tag="RPM73_000092"
     CDS             46513..47553
                     /locus_tag="RPM73_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDX6518491.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(47585..48778)
                     /gene="lplT"
                     /locus_tag="RPM73_000093"
     CDS             complement(47585..48778)
                     /gene="lplT"
                     /locus_tag="RPM73_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDX6518492.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(48771..50930)
                     /gene="aas"
                     /locus_tag="RPM73_000094"
     CDS             complement(48771..50930)
                     /gene="aas"
                     /locus_tag="RPM73_000094"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDX6518493.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            51516..52547
                     /gene="galR"
                     /locus_tag="RPM73_000095"
     CDS             51516..52547
                     /gene="galR"
                     /locus_tag="RPM73_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDX6518494.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(52554..53816)
                     /gene="lysA"
                     /locus_tag="RPM73_000096"
     CDS             complement(52554..53816)
                     /gene="lysA"
                     /locus_tag="RPM73_000096"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDX6518495.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDNIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            53938..54873
                     /gene="lysR"
                     /locus_tag="RPM73_000097"
     CDS             53938..54873
                     /gene="lysR"
                     /locus_tag="RPM73_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDX6518496.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(54860..55552)
                     /gene="ygeA"
                     /locus_tag="RPM73_000098"
     CDS             complement(54860..55552)
                     /gene="ygeA"
                     /locus_tag="RPM73_000098"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDX6518497.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(55681..57099)
                     /gene="araE"
                     /locus_tag="RPM73_000099"
     CDS             complement(55681..57099)
                     /gene="araE"
                     /locus_tag="RPM73_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDX6518498.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13983 a        15443 c        14172 g        13702 t
ORIGIN      
        1 atctctcaca accagatggg gtatttggat taaagaagag gcttaagttg attaaggaat
       61 tccgcaagat ttgaggctaa atgcgtccgt ttacgtgttc ccagcgtctc tttgcacact
      121 tcaccggaga gattacacac cgagatgacc tctaactctt cttccagcgt cgcgataaat
      181 agcgtgggcg gcagttttag ccttttctga gtcaccagat gaccaatcag attttcctgc
      241 acccgacgga agtcgtcttc actccaggtt tgcagcaatg tcagtttgat atcgccaaac
      301 tgcgcgtgca tatccccggc aaactgagtc gtataaaagg tatgaattgt gggttgtatc
      361 acaatatcaa aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa cggctggggt
      421 tgccagtata cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac accgtacagt
      481 tcctcactta acggccagct tttatgctct tcatgccacg catcgcagta gcgtgcagta
      541 aaatctttca gggcttgtgc ggtcaaatcg tccactaatt tctctcttca cttaaaccag
      601 atacacttgt cgtttagttt atctggttta tgacggtgaa acatgtcttc ttatgcaaac
      661 catcaggcac ttgcgggcct gactcttgga aaatcaaccg attaccggga tacctatgac
      721 gccagcctac tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg tctgaaagcg
      781 gataacctgc cttttcacgg tacggatatc tggacgctgt atgaactttc ctggctgaat
      841 gcgaaaggtt tgccgcaggt cgctgtcggt catgttgaac ttgattacac cagcgtaaat
      901 ctgattgagt cgaagagttt taagctctat ctcaacagtt ttaaccagac gcgttttaat
      961 aactgggatg aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc ccagggtaag
     1021 gttagcgtgg cgttatatcg tcttgatgaa ctggaaggcc agccgatagg tcatttcaat
     1081 ggcacctgca ttgatgacca ggatatcact atcgataact atgaattcac tactgactat
     1141 ctggagaatg ccaccagcgg tgaaaaagta gtggaagaga cgcttgtcag ccacctgctg
     1201 aaatcaaact gcctgatcac ccatcaacca gattggggtt cgatccaaat tcagtatcgt
     1261 ggacgccaaa ttgacagaga aaaactgctg cgttacctgg tctcattccg tcatcacaac
     1321 gagttccacg aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt ctgccagcca
     1381 gagaaattaa gcgtttacgc acgttatacc cgtcgtggcg gtctggacat taacccgtgg
     1441 cgcagtaata gcgattttgt cccatcgacc acaagactgg ttcggcaata aattttttct
     1501 caattttgcg tgctggattc acgcagaagg ttgtgaaagg tcatcaggca gggctattgt
     1561 aatcaaaggg aatgacgata ttcgtcccat aaggagtttt tcttgattac acatattagc
     1621 ccgcttggct ccatggatat gttgtcgcag ctggaagtgg atatgcttaa acgcaccgcc
     1681 agcagcgacc tctatcaact gtttcgcaac tgttcacttg ccgtactgaa ctccggtagt
     1741 ttgaccgata acagcaaaga attgctgtct cgttttgaaa atttcgatat taacgtcctg
     1801 cgccgtgaac gcggcgtaaa gctggaactg attaatcccc cggaagaggc ttttgtcgat
     1861 gggcgaatta ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga cattctcttc
     1921 gtttacgggc aaatccataa taccgttcgt tttcccaacc tgaatctcga caactccgtc
     1981 cacatcacta acctggtctt ttccatcttg cgtaacgctc gcgcgctgca tgtgggtgaa
     2041 gcgccaaata tggtggtctg ctggggcggt cactcaatta acgaaaacga gtatttgtat
     2101 gcccgtcgcg tcggaaacca gctgggcctg cgtgagctga atatctgcac cggctgtggt
     2161 ccgggagcga tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca gcagcgttac
     2221 aaagacagtc gttttattgg tatgacagag ccgtcgatta tcgccgctga accgcctaac
     2281 ccgctggtca acgaattgat catcatgccg gatatcgaaa aacgtctgga agcgtttgtc
     2341 cgtatcgctc acggcatcat tatcttccct ggaggtgtgg gtacggcaga agagttgctg
     2401 tatttgctgg gaattttaat gaacccggcc aacaaagatc aggttttacc attgatcctc
     2461 accggcccga aagagagcgc cgactacttc cgcgtactgg acgagtttgt cgtacatacg
     2521 ctgggcgaaa acgcgcgccg ccattaccgc atcatcattg atgacgccgc tgaagtcgct
     2581 cgtcagatga aaaaatcgat gccgctggtg aaagaaaatc gccgcgacac tggtgacgct
     2641 tacagcttta actggtcaat gcgcattgca ccagatttgc aaatgccgtt tgagccgtct
     2701 cacgagaata tggctaatct gaagctttac ccggatcaac ctgttgaagt gctggctgcc
     2761 gacctgcgcc gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt cggtattcgc
     2821 gccattgaag agtttggtcc ttacaaaatc aacggcgata aagagattat gcgtcgtatg
     2881 gacgacctgc tacagggttt tgttgcccag catcgtatga agttgccagg ctcagcctac
     2941 atcccttgct acgaaatctg cacgtaatct ccgctccccc ggtgacgttt gttgccgggt
     3001 tttctcgttt ttggtcactt actcatcaac tcatttcatt tgttatatga atgtttctta
     3061 ccaccctcac gcgacaaata tcatcacagt taatatgtca tacaatttat gttgcaacgc
     3121 aaacgtttcc ctattttcat aaaccgtatt ttatcgctat gaaaaagaaa ttatcgccat
     3181 gattaactaa aagtattgat tttttcagtt caacctacat atattgcgcg ccccggaaga
     3241 agtcagatgt cgtttaatgg gcaaatattg cccttaaatt ctcttttact tttgatttac
     3301 agagtaaagc gttgggataa tctatcttcc aagtagatta ttgtatttga gatcaagatc
     3361 actgatagat acataacttg tgtgtatctt tccgccctca aattattacg gcggtaaatg
     3421 attaagccat cgccgataga cagatttcat ttttacggtc aggcaccttc ccgggctgaa
     3481 ctggctaaaa gctgaattat ttgcattcct ccaggagaaa tagatggaaa cgactcaaac
     3541 cagcacgatt gcgtcgaaag actctcgtag tgcctggcgc aagacagaca ccatgtggat
     3601 gctgggcctt tacggcacgg caatcggcgc gggcgtgctg ttcctgccaa tcaacgccgg
     3661 tgttggcggt atgatcccgc tgatcatcat ggctatcctt gcgttcccga tgacgttttt
     3721 tgctcaccgc ggcctgactc gcttcgtact gtctggtaaa aacccgggcg aagacatcac
     3781 cgaggttgta gaagaacact ttggtattgg cgcaggtaaa ctgattaccc tgctctactt
     3841 cttcgctatc tacccgatcc tgctggttta tagcgtggca atcaccaata cagttgaaag
     3901 cttcatgtct caccagctgg gtatgacgcc accgccgcgt gcgattctgt cgctgatcct
     3961 gatcgtgggt atgatgacca tcgttcgctt tggtgagcag atgatcgtta aagcgatgag
     4021 tattctggta ttcccgtttg ttggcgtact gatgctgctg gctctgtacc tgatcccgca
     4081 gtggaacggc gcagcactgg aaacgctgtc tctggacact gcatctgcaa ccggaaacgg
     4141 tctgtggatg accctgtggc tggcaattcc ggtaatggtg ttctcgttca accactctcc
     4201 gatcatctct tctttcgccg ttgcgaagcg tgaagagtac ggcgatatgg cagaacagaa
     4261 atgctcgaag atcctggcat tcgcacacat catgatggtg ctgaccgtaa tgttcttcgt
     4321 cttcagctgc gtactgagcc tgactccggc agacctggct gcggctaaag agcagaacat
     4381 ctcgattctg tcttacctgg ctaaccactt taacgcaccg gttatcgcgt ggatggctcc
     4441 gattatcgcg attatcgcta tcaccaaatc cttcctcggc cactacctgg gcgcacgtga
     4501 aggcttcaac ggtatggtga ttaaatctct gcgtggtaaa ggtaagtcta tcgaaatcaa
     4561 caagctgaac cgtatcactg cgctgttcat gctggtaacg acctggattg ttgccaccct
     4621 gaacccgagc atcctgggta tgattgaaac cctgggcggc ccaatcatcg cgatgatcct
     4681 gttcctgatg ccgatgtacg caattcagaa agtaccggca atgcgtaagt acagcggtca
     4741 catcagcaac gtattcgttg tcgtgatggg tctgattgca atctccgcaa tcttctactc
     4801 tctgttcagc taagtccttt cgcgccgctt tcgggcggcg cttcctccgt tttaacgcga
     4861 tgtatttcct atgattagcg tattcgatat tttcaaaatc ggcattggcc cttccagttc
     4921 tcataccgtt ggaccaatga aagcgggtaa acaatttacc gacgatctga ttgcccgtaa
     4981 tctgcttaaa gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct ctctgaccgg
     5041 taaaggccac cacactgata tcgccattat tatgggcctg gcgggtaacc tgccggatac
     5101 cgtggatatc gattccatcc ctggttttat tcaggatgtg aatactcatg gtcgcctgat
     5161 gctggcaaac ggtcagcatg aagtggagtt cccggttgat cagtgcatga acttccatgc
     5221 agacaacctt tctctgcatg aaaacggtat gcgcattacc gcgctggcgg gcgataaagt
     5281 cgtttacagc cagacttact actctattgg cggtggcttt atcgttgatg aagagcattt
     5341 tggccagcag aatagcgcac cggttgaagt tccttatccg tacagttcag cagccgatct
     5401 gcaaaaacat tgtcaggaaa ccgggctgtc actctctggc ctgatgatga aaaacgagct
     5461 ggcgctgcac agcaaagaag agctggaaca gcacctggcg aacgtctggg aagtcatgcg
     5521 cggcggtatt gagcgcggta tttctaccga aggcgtgttg cctggcaaat tgcgcgtacc
     5581 acgccgtgct gcggcactac gccggatgct ggtcagccag gataaaacca ccactgaccc
     5641 gatggcggtt gttgactgga tcaacatgtt tgcactggca gtgaacgaag agaacgccgc
     5701 tggcggtcgc gtggtgactg cgccgactaa cggtgcgtgc gggattatcc cggctgtgct
     5761 ggcgtactac gataagttta tccgcgaagt gaacgctaac tcactggctc gttacctgct
     5821 ggtagccagt gccattggtt ctctttataa gatgaacgcg tcaatttctg gtgctgaagt
     5881 cggctgccag ggtgaagttg gtgtggcgtg ctcaatggcg gcggctggtc tggcagaact
     5941 gttaggtgca agcccggcgc aggtgtgcat cgcggcggaa atcgccatgg agcacaacct
     6001 cggtctgacg tgtgacccgg tcgccggaca ggtccaggtg ccatgcatcg agcgtaacgc
     6061 cattgcggca gtaaaagcgg tgaacgccgc acgtatggcg ctacgccgta ccagcgagcc
     6121 gcgcgtctgc ctcgataaag ttatcgaaac catgtacgaa acaggtaaag atatgaacgc
     6181 caagtaccgc gaaacctctc gcggcggcct ggcaatgaag atcgttgcct gcgattaatc
     6241 gctctccaga ggcctcgttt tgcgaggcct cttcccgatt tctcatccag ccgtagcctg
     6301 ttccggcatc gaatgttacc cttatcgcct gatctttaag ggggttatcg tggctgttca
     6361 tttgcttatt gtcgatgcac tgaatcttat tcgtcgcatt catgccgttc agggatcgcc
     6421 ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt atgcacagcc agccaaccca
     6481 cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc tggcgtcatc agcgtttacc
     6541 agattacaaa gcgggtcgac cgccaatgcc ggaagagttg cacgacgaga tgcctgcatt
     6601 acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca accagcggca acgaagccga
     6661 tgacttagcc gccacgctgg cggtcaaagt gacacaggcc gggcatcagg caacgattgt
     6721 ttcgacagat aaaggctact gtcagttact ttcaccgaca ttacgtattc gtgattactt
     6781 ccagaaacgt tggctggatg cgccatttat cgataaagaa tttggcgttc aaccgcagca
     6841 gttgcccgat tactggggac ttgcggggat cagcagttca aaggtaccgg gtgttgcggg
     6901 aatcggacca aaaagcgcca cgcagctgct ggtcgagttt cagagtctgg aagggatata
     6961 tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa ttagaaaccc ataaagagat
     7021 ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat ttgcatatcg acggcaattt
     7081 acagcaattg cggttggtac ggtaacggcg agccggatac gccgcaaacg tcgtatccgg
     7141 cattatcaca tcagcgcatt taccaggcgg tatggtaaag ctctacaata tcctcaagcg
     7201 ttgcttcacg cgggttgcca ccggtacaaa catcatccag tgccgcctgc gccagtgccg
     7261 gaatgtcttc cttgcgtaca ccaacatcac gcaaatgtgg cggaataccg acatcacggt
     7321 tgagagcaaa caccgcttca acagcggcat tacgcgcctc ttccaggctc ataccttcca
     7381 ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat
     7441 agcgcatgac atgcggtaac aggatggcgt tcgcaacacc gtgtggagtg ttgtaaaacg
     7501 cgcccagtgg atgcgccata ccatgcacca accctaaccc aacattcgag aagcccatac
     7561 ccgcaacata ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc
     7621 ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg cagtgcatcg gttagcgccc
     7681 acgcgccacg ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcag
     7741 ctttcagcgc tggaggcata ccatccatca tgtcagcgtc aataaacgcc acctgcggga
     7801 tatcatgcgg atcaacgcaa acaaacttgc gccgtttctc ttcgtcagtg atcacgtagt
     7861 taatggtcac ttctgccgca gtacctgctg tggtaggaat tgccagaatc ggtacactgg
     7921 gtttattggt cggggaaagc ccttccaggc tacgcacatc ggcaaactcc gggttgttgc
     7981 tgataatgcc aatcgcttta caagtatcct gtggagaacc accaccaata gcgatcaggt
     8041 aatccgcgcc gctattctgg aatacaccga gcccttcttt gacgacagta attgttgggt
     8101 tgggcactac gccgtcgtaa atcgcccatg ccagccctgc agcatccatc ttatcggtca
     8161 ctttcgccac cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat
     8221 aaccacggcg tttcacctca tcggttaaag ccccaacagc accccgacca aaccatgccg
     8281 tttcgttcag aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact
     8341 cttcaatccg taacccatag gttttgaatt tctccagcac tacggcaatc tcttcatcgc
     8401 tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgc gccagcactt
     8461 caacttcatg cgccagccat aacgcttttt ccagattcac ctcacaagcg ataagcccat
     8521 gatgttgtaa caaagttgcc ttacgatttt tgagagcctg cgcaacatgt tcagaaagtt
     8581 cacgtgttcc aaaggtcgca taaggcgcgc aaggaataga attaccgcca gccgccgcaa
     8641 tcatgtagtg aatagcgggg atcgatcggt taagaatgga aactgccgtg caatgaacgg
     8701 catgattgtg aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac
     8761 gccattcgct tgaggggagc tttccttcct catgtttacc gttgccatca ataaagacaa
     8821 tatgcgactc cgtcagtttt tcatatggaa tgcctgtagg cgtaatcagc atcccatcct
     8881 gataacgtac actgacgttc cccgctgtcc cctggttcag tcccaggcgg gtcatttcca
     8941 ggcaagtgtc aataatctga cgagcaagtt tatttcgttc cattagctac ctctctctga
     9001 ttcaaaacag ggcaataatg ttgttccttt cacactattg aattagccgt ttaattaccc
     9061 accatcttct tcctgattaa ccagaaagaa attcacaagc ttatattttg tgacctggtt
     9121 caactaatca cagtaaataa ctgcaagttc tctttttata accccattaa aaatgaccgc
     9181 tcttaaaaat atttatcaaa acggtcattt ttctattcac tcaagccagg aatgcccgtt
     9241 ttcggcataa acaattaata cggtcatctg gtttgtgttt tttatgattt attttctgaa
     9301 acgggcatga aatttcgatt attaaagtga tggtagtcac ataaagtcac cttccagcta
     9361 ataagtgtga ccgccgtcat attacagagc gttttttatt tgaaaatgaa tccatgagtt
     9421 catttcagac aggcaaatat tcactgatat gaagcccgaa ctcgctggtt ttgcactttt
     9481 gaaaacataa ccgattacgt gcttaagctt ctgaacctaa gaggatgcta tgggaaacac
     9541 atcaatacaa acgcagagtt accgtgcggt agataaagat gcagggcaaa gcagaagtta
     9601 cattattcca ttcgcgctgc tgtgctcact gttttttctt tgggcggtag ccaataacct
     9661 taacgacatt ttattacctc aattccagca ggcttttacg ctgacaaatt tccaggctgg
     9721 cctgatccaa tcggcctttt actttggtta tttcattatc ccaatccctg ctgggatatt
     9781 gatgaaaaaa ctcagttata aagcagggat tattaccgga ttatttttat atgccttggg
     9841 cgctgcatta ttctggcccg ctgcagaaat aatgaactac accttatttt tagttggcct
     9901 atttattatt gcagccggat taggttgtct ggaaactgcc gcaaaccctt ttgttacggt
     9961 attagggccg gaaagcagtg gtcacttccg cttaaatctt gcgcaaacat ttaactcgtt
    10021 tggcgcaatt atcgcggttg tctttgggca aagtcttatt ttgtctaacg tgccacatca
    10081 atcgcaagac gttctcgata aaatgtctcc agagcaattg agtgcgtata aacacagcct
    10141 ggtattatcg gtacagacac cttatatgat catcgtggct atcgtgttac tggtcgccct
    10201 gctgatcatg ctgacgaaat tcccggcatt gcagagtgat aatcacagtg acgccaaaca
    10261 aggatcgttc tccgcatcgc tttctcgcct ggcgcgtatt cgccactggc gctgggcagt
    10321 attagcgcaa ttctgctacg ttggcgcaca aacggcctgc tggagctatt tgattcgcta
    10381 cgctgtagaa gaaattccag gtatgactgc aggctttgcc gctaactatt taaccggaac
    10441 catggtgtgc ttctttattg gtcgtttcac cggtacctgg ctcatcagtc gcttcgcacc
    10501 acacaaagtc ctggccgcct acgcattaat cgctatggca ctgtgcctga tctcagcctt
    10561 cgctggcggt catgtgggct taatagccct gactttatgc agcgccttta tgtcgattca
    10621 gtacccaaca atcttctcgc tgggcattaa gaatctcggc caggacacta aatacggttc
    10681 gtccttcatc gttatgacca tcattggcgg cggtattgtc actccggtca tgggttttgt
    10741 cagtgacgcg gcgggcaaca tccccactgc tgaactgatc cccgcactct gcttcgcggt
    10801 catctttatc tttgcccgtt tccgctctca aacggcaact aactgaacat attttccgaa
    10861 taaagtgagg aatctgtaat gaaaaaaatc agcttaccga aaattggtat ccgcccggtt
    10921 attgacggtc gtcgcatggg tgttcgtgag tcgcttgaag aacaaacaat gaatatggcg
    10981 aaagctacgg ccgcactgct gaccgagaaa ctgcgccatg cctgcggagc tgccgtcgag
    11041 tgtgtcattt ccgatacctg tatcgcgggt atggctgaag ccgctgcttg cgaagaaaaa
    11101 ttcagcagtc agaatgtagg cctcaccatt acggtaacgc cttgctggtg ctatggcagt
    11161 gaaaccatcg acatggatcc aacccgcccg aaagccattt ggggctttaa cggcactgaa
    11221 cgtcccggcg ctgtttacct ggcagcggct ctggcagctc acagccagaa aggcatcccg
    11281 gcattctcca tttacggtca tgacgttcag gatgccgatg acacatcgat tcctgccgat
    11341 gttgaagaaa aactgctgcg ctttgcccgc gccggtttgg ccgtcgccag catgaaaggt
    11401 aaaagctatc tgtcgctggg cggcgtttcg atgggtatcg ccggttccat tgttgatcac
    11461 aacttctttg aatcctggct gggaatgaaa gtccaggcgg tggatatgac cgaactgcgt
    11521 cgccgtatcg atcagaagat ttacgacgaa gccgaactgg aaatggcact ggcctgggct
    11581 gataaaaact tccgctatgg cgaagatgaa aataacaaac agtatcaacg caatgccgag
    11641 caaagccgcg cagttctgcg cgaaagttta ctgatggcga tgtgtatccg cgacatgatg
    11701 caaggcaaca gcaaactggc cgatattggt cgcgtggaag aatcacttgg ctacaacgcc
    11761 atcgctgcgg gcttccaggg gcaacgtcac tggaccgatc aatatcccaa tggtgacacc
    11821 gccgaagcga tccttaacag ttcatttgac tggaatggcg tgcgcgaacc ctttgtcgtg
    11881 gcgaccgaaa acgacagtct taacggcgtg gcaatgctaa tgggtcacca gctcaccggc
    11941 accgctcagg tatttgccga tgtgcgtacc tactggtcac cagaagcaat tgagcgtgta
    12001 acggggcata aactcgatgg actggcagaa cacggcatca tccatttgat caactccggt
    12061 tctgctgcgc tggacggttc ctgtaaacaa cgcgacagcg aaggtaaccc gacgatgaag
    12121 ccgcactggg aaatctctca gaaagaggct gacgcttgcc tcgccgctac cgaatggtgc
    12181 ccggcgatcc acgaatactt ccgtggcggc ggttactctt cccgcttcct taccgaaggc
    12241 ggcgtcccgt tcaccatgac tcgtgtcaac atcatcaaag gcctgggacc ggtactgcaa
    12301 atcgcggaag gctggagcgt ggaattgccg aaggatgtgc atgacatcct caacaaacgc
    12361 accaactcaa cctggccaac cacctggttt gcaccgcgcc tcaccggtaa agggccgttt
    12421 acggatgtgt actcgataat ggcgaactgg ggcgctaacc atggggttct gaccatcggc
    12481 cacgttggcg ctgactttat cactctcgcc tccatgctgc gtatcccggt atgtatgcac
    12541 aacgttgaag agaccaaagt gtatcgtcct tctgcctggg ctgcgcacgg catggatatt
    12601 gaaggccagg attaccgcgc ttgccagaac tacggcccgt tgtacaagcg ttaatacatt
    12661 actccctgat gtgatgcccg gtcgctccgg ctaccgggcc tgaacaagca agagtggtta
    12721 gccggataag caatgttatc cggctatatt gcaggagcga ttatgaaaca agaagttatc
    12781 ctggtactcg actgtggcgc gaccaatgtc agggccatcg cggttaatcg gcagggaaaa
    12841 attgttgccc gcgcctcaac gcctaatgcc agcgatatcg cgatggaaaa caacacctgg
    12901 caccagtggt ctttagacgc cattttgcaa cgctttgctg attgctgtcg gcaaatcaat
    12961 agtgaactga ctgaatgcca catccgcggt atcgccgtca ccacctttgg tgtggatggc
    13021 gctctggtag ataagcaagg caatctgctc tatccgatca ttagctggaa atgtccgcga
    13081 acagcagcgg ttatggacaa tattgaacag ttgatctccg cacagcggtt gcaggctatt
    13141 tctggcgtcg gagcctttag tttcaatacg ttatataagt tggtgtggtt gaaagaaaat
    13201 catccacaac tgctggaacg cgcgcacgcc tggctcttta tttcgtcgct gattaaccac
    13261 cgtttaaccg gcgaattcac tactgatatc acgatggccg gaaccagcca gatgctggat
    13321 atccagcaac gcgatttcag tccgcaaatt ttacaagcca ccggtattcc acgccgactc
    13381 ttccctcgtc tggtggaagc gggtgaacag attggtacgc tacagaacag cgccgcagca
    13441 atgctcggct tacccgttgg cataccggtg atttccgcag gtcacgatac ccagttcgcc
    13501 ctttttggcg ctggtgccga ccaaaatgaa cccgtgctct cttccggtac atgggaaatt
    13561 ttaatggtcc gtagcgccca ggttgatact tcgctgttaa gtcagtacgc cggttccacc
    13621 tgcgaactgg atagccaggc agggctgtat aacccaggta tgcaatggct ggcatccggc
    13681 gtgctggaat gggtgagaaa actgttctgg acggctgaaa caccctggca aatgttgatt
    13741 gaagaggctc gtctgatcgc gcctggcgcg gatggcgtga aaatgcagtg tgatttattg
    13801 tcgtgtcaga acgctggctg gcaaggagtg acgcttaata ccacgcgggg gcatttctat
    13861 cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca atctacagat gctggaaaaa
    13921 atcgggcact ttaaggcctc tgaattattg ttagtcggtg gaggaagtcg caacacattg
    13981 tggaatcaga ttaaagccaa tatgcttgat attccggtaa aagttctcga cgacgccgaa
    14041 acgaccgtcg caggagctgc gctgttcggt tggtatggcg taggggaatt taacagcccg
    14101 gaagaagccc gcgcacagat tcattatcag taccgttatt tctacccgca aactgaacct
    14161 gaatttatag aggaagtgtg aaatgctgaa aacaatttcg ccgttaattt ctcccgaact
    14221 attgaaagtg ctggcagaga tgggacatgg agatgaaatt attttttccg atgctcactt
    14281 tcccgcccat tcgatgggac cgcaggtgat ccgcgctgat ggcctgttgg tgagcgactt
    14341 gctccaggcg attatcccgt tatttgaact ggacagttat gcaccgccgc tggtgatgat
    14401 ggcggcggta gaaggtgaca ctctcgatcc tgaagtagaa cgacgttacc gtaatgcgct
    14461 ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat cgttttgcgt tttatgaacg
    14521 ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga gcgaagtacg ggaatattct
    14581 tttaaaaaaa ggggtaacac cgtaatctca taccggtacg cccgcatgac gcgggcggtt
    14641 atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag caagcgatag tcgacctgct
    14701 gctgaaccat accagcctga ccacggaagc tctctctgaa cagctaaagg tcagtaaaga
    14761 aaccattcgt cgcgatctca atgaattaca gacgcagggt aaaattctgc gcaatcatgg
    14821 acgcgctaaa tatatccacc gtcaaaatca agacagtggc gatccctttc acatcaggct
    14881 gaaaagccat tatgcgcata aagcagatat cgcgcgcgag gcgctcgcgt ggattgaaga
    14941 agggatggtg atagccttag acgccagttc aacttgctgg tatctggcac gccagttgcc
    15001 tgacatcaac attcaggtct tcaccaatag ccatccgatt tgccatgaac tcggtaaacg
    15061 cgaacgcatt caactgatca gttccggcgg cacacttgag cgcaaatatg gctgttacgt
    15121 caatccctcg ctgatttccc aacttaaatc gctggaaatc gatctgttta ttttttcttg
    15181 tgaagggatc gatagcagcg gcgcactgtg ggactccaat gcgatcaacg ctgattacaa
    15241 atcgatgcta ttaaaacgtg ccgcgcaatc gttgttattg attgataaaa gtaaatttaa
    15301 tcgttcaggg gaagcccgca tcgggcatct ggatgaggta acgcacatta tttctgatga
    15361 gcgccaggtt gcaacttctt tggtaacagc ctgacggaaa gggtagcagg ccggagacga
    15421 cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac ccaccgcagc ccagatgcgg
    15481 cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact gccgcgcctg aatctgagca
    15541 tttatgccat gttcatcaag ctgtgcctga atatacgcca gattgtgtga cacttcttcg
    15601 tagcgttttt tcatcggcag tttgaggttg aaaatggttt cacggcacca gccattaacc
    15661 agccactgcg ccatcaatgc cgcaactttc gccggttttt caaccatatc gcataccatc
    15721 caggagatat tgctgcgcgt cggacggaat ttgaaaccgt cttcccgcag ccacgtcacc
    15781 tgtccggtat ccatcagact ttgcgccatc gggccgttgt cgacggaata aacccacatg
    15841 ttgcgcttca ccagttggta ggtccagccg ccagggcaag cgcctaaatc caccgcccac
    15901 atcccgttcg ccaggcgttc atcccactca tccgcaggaa taaacacatg aaatgcctct
    15961 tccagtttga gcgtggaacg actcggcgca tctgccggaa atttcaggcg cggaatgccc
    16021 atatagaacg gcgaattatt gttgctgtat gagtaaccgg tatagcagca gcctggtgca
    16081 ataaagaata catgcacaac cggacgcttc ggcgtttcat agttcgccag caccccggca
    16141 tcgcgcaagg cagcgcgtag cggaacagta aatttacggc agaatttcag taactctttg
    16201 ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc ccttctctac tacgccctgt
    16261 aacatgccga caatgggggt aatacgatct tctggcggca aatgctgcag gagttccccc
    16321 accacaaacc actggcgggc aaaaattaat gaactgaacg gcagctcacg gattaactta
    16381 tcgccatcat caggttgata acattcataa atgacataac ccgcattctc tttcacgcgg
    16441 gcaaaaccga aaatttcccg ctggccggct ttatcggtaa tttctgcggc gcactctttt
    16501 tcaaagcccg gacggcacag caatacaacc ttattcatga cttacgccct tgcgctttaa
    16561 acggatagca ccaactaaca ttaacgccca gcccgcgagg aagctcacgc cgccaaccgg
    16621 agtgacaaac gcccacaaac gcagatggga cagcgccagg caataaaggc tgccgctgaa
    16681 caacaccgtg cctaacgcga ggaaaacgct actccagtaa aaccagatac tgatgcgacg
    16741 ctgcattgcc accgccagac ctaagatcgc cagcgtatga aacgcctggt attcgaggcc
    16801 ggtctggatc cagcccatct caacggcccc catggtttta cttaacacat gcgcgccaaa
    16861 agcgcccaga gccacaaaaa tgaagccgct aatggcggcg aaaatcagca taaaacggct
    16921 ggtcatggtc gtaccctacg taaattattg ttcataacga aagcggaatt tttcttgttc
    16981 agcagcagct ttcgccagga tccattgacg aaaggcggct attttaccca gttctgcctg
    17041 gctgtcatga caaaccagat aaaaagcgtt tttactgacc agaacatcat taaacgggca
    17101 aacaagacgt ccggcctcga tttcagattg cgccatcacg ttatttgcca gcgccactcc
    17161 ctgcccgtga atagccgctt gcagcaccat ggcgctatgg ctaaaaattg gcccttgctg
    17221 aacgttgata tgatttaacc ccaattgtcg ggtatatgtc tgccagtcac ggcgtgacgc
    17281 atcatgtaat aacgtatgtt tagccagatc ttccggggtc ttcaagggtt tttcgccagt
    17341 cagcagtagc ggcgaacaca ccggcaataa atattcggcg taaagttttt ccacccgtag
    17401 ccccggccag ttgccccgac cataaaatat cgccacatca acatcatccg ccagcttatc
    17461 ttcctgacga tcaaccgcct ggattcgaac gtcaattccc ggataagctg aattaaagct
    17521 ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg agtaaactga ccgtcaacgc
    17581 ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg gttaattgcg aaaatatctc
    17641 tttgatatcg aggaaatagc tttgcccttc ctcggtcagc aggagtgaac gattacggcg
    17701 gcggaacagt tttagcccca aaaaatcctc aagagacttg atttgatgac ttactgcggc
    17761 ctgggtcaca aaaagctctt ctgctgcgcg agtgaaactt aaatggcgtg ctgcggcatc
    17821 aaaaactcgt aaggcattta gcggtggtaa tcgtttagac atggctttta aactttgatg
    17881 ttaaatgaat ttaacaatta gatcacacta tgtaacctat tagttttttt aatctgagcc
    17941 attataaatt gtccgttgag cttctaccag caaataccta tagtggcggc acttcctgag
    18001 ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa cttttggctt acggttgtga
    18061 tgttgtgttg ttgtgtttgc aattggtctg cgattcagac catggtagca aagctacctt
    18121 ttttcacttc ctgtacattt accctgtctg tccatagtga ttaatgtagc accgcctaat
    18181 tgcggtgctt ttttttacct tgcgatcgcg aattactgat ccagttcgac catctctttc
    18241 acgtcagtac ggttgatctg ctgtttgttg ccattagcgt ctttatacga aatcataccg
    18301 gtatcgttat cagtctgtgg tttgccgtca gagacgatgg tacgtccgtc attggtgtgc
    18361 atcacatagt tcgaaccgga acaggcgctc agggcaaaag tcagcataca ggcagaaata
    18421 attgcggcag tctttttcat catcttctcc ttaaagcgat taataaatct taatagtatt
    18481 gattaacagg ctaaaattaa cgcctaacac tattcagcat atgttacttg cgcggcttcg
    18541 ccaggatatc cagataattc tgatggttag cactctcctt gtatcaaagt gaattttgcg
    18601 tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt tttaatcccg cgcagtttcg
    18661 cgcccagttt cccgcactac aggatgcggg cgtctatctc gacagcgccg cgaccgcgct
    18721 taaacctgaa gccgtggttg aagccaccca acagttttac agtctgagcg ccggaaacgt
    18781 ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg cgttatgaag ctgcacgaga
    18841 gaaagtggcg caattactga atgcaccgga tgataaaact atcgtctgga cgcgcggcac
    18901 cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt ccgcgtctgc aaccgggcga
    18961 tgagattatt gtcagcgtgg cagaacacca cgccaacctc gtcccctggc tgatggtcgc
    19021 ccaacaaact ggagccaaag tggtgaaatt gccgcttaat gcgcagcgac tgccggatgt
    19081 cgatttgttg ccagaactga ttactccccg tagtcggatt ctggcgttgg gtcagatgtc
    19141 gaacgttact ggcggttgcc cggatctggc gcgagcgatt acctttgctc attcagccgg
    19201 gatggtggtg atggttgatg gtgctcaggg ggcagtgcat ttccccgcgg atgttcagca
    19261 actggatatt gatttctatg ctttttcagg tcacaaactg tatggcccga caggtatcgg
    19321 cgtgctgtat ggtaaatcag aactgctgga ggcgatgtcg ccctggctgg gcggcggcaa
    19381 aatggttcac gaagtgagtt ttgacggctt cacgactcaa tctgcgccgt ggaaactgga
    19441 agctggaacg ccaaatgtgg ctggtgtcat aggattaagc gcggcgctgg aatggctggc
    19501 agattacgat atcaaccagg ccgaaagctg gagccgtagc ttagcaacgc tggcggaaga
    19561 tgcgctggcg aaacgtcccg gctttcgttc attccgctgc caggattcca gcctgctggc
    19621 ctttgatttt gctggcgttc atcatagcga tatggtgacg ctgctggcgg agtacggtat
    19681 tgccctgcgg gccgggcagc attgcgctca gccgctactg gcagaattag gcgtaaccgg
    19741 cacactgcgc gcctcttttg cgccatataa tacaaagagt gatgtggatg cgctggtgaa
    19801 tgccgttgac cgcgcgctgg aattattggt ggattaatga caaacccgca attcgccgga
    19861 catccgttcg gcacaaccgt aaccgcagaa acgttacgca ataccttcgc accgttgtcg
    19921 caatgggaag ataaatatcg ccagttgatc atgctgggga aacagcttcc ggctttgcca
    19981 gacgagttaa aagcgcaggc taaagagatt gccggatgcg aaaaccgcgt ctggctggga
    20041 tataccgtga ctgaaaacgg taaaatgcac ttctttggcg atagcgaagg gcgcattgtg
    20101 cgcggcctgt tggcggtgtt gttgactgcc gttgagggga aaaccgccgc cgaattgcag
    20161 gcacagtcgc cgctggcatt gtttgatgag ctgggattgc gtgcacagct tagcgcctca
    20221 cgcagccagg ggttgaatgc gttaagcgag gcgattatcg ctgcggcgaa gcaggtttaa
    20281 gcacagaacc aggtcggata agacgttcgc gtcgcatccg acctgattgt ttaaccctga
    20341 cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa ccgcaacaaa cccaaaggtg
    20401 gcggtcacca tcgttgccgc gccaaatcca gatgcacaat ccatccgctt cggcccttct
    20461 gccgtggctt tcatcgcaca cacagtaccg tctgactgcg ggtacaccag cgcttcagta
    20521 gaaaacacgc aatcgacgcc gagcttacct ttactgtttt tcactacgcc aaaatcgctt
    20581 ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa tcgttttcgc cagatcggta
    20641 acctgaatct gcgtcgggtc aatctgccca cccgcgccac cggtcgtgac cagcgggatt
    20701 ttattgcgcc gacaataagc aatcagcgcc gctttgggcc gtacactatc aatggcatca
    20761 atcacgtacg aataacccac gctcatatac tgcgctacgt tatccggcgt cacgaaatca
    20821 tccaccaccg ttacacggca ctccgggtta atctggcgaa tacgctccgc catgacttcc
    20881 gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt gccgattggt attggtgacg
    20941 cacacatcat ccatatcgat aagcgtgatt gcgccaatcc ccgtgcgcgc cagcgcttcc
    21001 gccgcccagg aaccgacacc accgatacca accacacaaa tatgcgcgtc agcaaacagt
    21061 tgcaacgctt tttcaccata cagacgcgct gtgccaccaa aacgctgacg ccatgcatca
    21121 ctaattacca cagacatagc acctcagaat taaaaagggt gaggttatcc ctcaccctgg
    21181 cgtatccgta aaaacggtat acagaataac acatcagccg ctaaagacgt tacctgcgcc
    21241 cggggcggtt ttcagcaccc agacacgtcc atagtggttg taccaacctg cgcgatgtcc
    21301 ggcttccggc ccgatccctt gatagatatc gaagtgttgg cctttgattg cgccaccgac
    21361 atccagcgcc accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag
    21421 cagcggcact tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac
    21481 agaagcacgg ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg
    21541 tttaaagaaa acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg
    21601 tgtttcgccc caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg
    21661 gtcgatcagc accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa
    21721 aaagttaagc ggactgccat caccaaagtc gatatacccg ctaccctgca catccataat
    21781 gaagttatcc atcagggagt tgctgtaagc gagaatatat ttatcactca gcgcccctgc
    21841 gtagatctcc gcacgagacg gcagacgacc acgttttggc ggcatacggt aaatcggata
    21901 ctggaactcg ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa
    21961 ctgcacgtta ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg
    22021 catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact
    22081 ctggttgcca tacagacgcg gtgacgaatt acggatatgg ttaatttgct cggcaaaatc
    22141 accggcgtta atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt
    22201 aaatttcccg tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag
    22261 cattgccaca accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt
    22321 cttcaggtta aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag
    22381 cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct ttagtgattt gtttttgttc
    22441 aaaatcatgc caaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    22501 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    22561 cgaaggtcgt cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt
    22621 tttttttgtt tctgctctcc caaaaaaaaa gcgccattca gcgccttttt atcatcccct
    22681 tctcgccagc gtcgccccat cggcaaaata cgctttaatt cccgcaagaa tagactccgc
    22741 aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa
    22801 cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg
    22861 ttcaacttga tttttatgca gcttgttgat tttaccgagc ttattcagta ccgctttacc
    22921 aaacttcagg ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac
    22981 atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc
    23041 cagatatttt gccgcagtac tggttgcacc tttggttgag agcgcaaaca cagaggaacc
    23101 gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg
    23161 tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat
    23221 gtacaccttc atattgccct ctttctcgat cagagagcgc aaacggcgag ctatttgcaa
    23281 taccacgtct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg
    23341 gccagggtca agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc
    23401 actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc
    23461 cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc
    23521 tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc
    23581 aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg
    23641 gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc
    23701 gatatccacc accacgcgtt ccgggttact caacgcgaac tgcttatatt tcagctgacg
    23761 atttgattct accgtcacac gggtgtagct ggacgcaggc cagacgcgca ccgccacgac
    23821 ctggctgacc gcagccagac tgacctgact tacgctcaat agccacatgg cacccgcgcc
    23881 ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa
    23941 aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga
    24001 cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat
    24061 aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat
    24121 acactaattt cgaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag
    24181 ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt
    24241 gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat
    24301 atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag
    24361 atcgacgcaa atctggccgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg
    24421 accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca actggacatc
    24481 actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca tatcaacgtc
    24541 gtcagtggca attttattat tgcccagccg ctgggcgtcg atgacggggt ggattactgc
    24601 catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact ggacagcggt
    24661 gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc
    24721 tcggaagaga ttgccactca actggccatc aaactgaaag ctgaaaagat gattggtttt
    24781 tgctcttccc aaggcgtcac taatgacgac ggtgatattg tctccgaact tttccctaac
    24841 gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg
    24901 cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc
    24961 agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg
    25021 cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt
    25081 attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag
    25141 cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc
    25201 tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt
    25261 cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag
    25321 gcgaagcaga gcggcttaag caaattattt gtgctgacca cgcgcagtat tcactggttc
    25381 caggaacgtg gatttacccc agtggatatt gatttactgc ccgagagcaa aaagcagttg
    25441 tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc gatgaaaatc
    25501 gtcggatgcg acatgcgtaa cactcgtacg tcgcatccgg caattacgtt tattcccgtg
    25561 aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt gccgcactta
    25621 atatgcgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc gcggtataaa
    25681 ccagctctcg cgttactacc ggcgtgcgtt ggctcggcaa aatcaacgcc gcatggtcga
    25741 actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc
    25801 gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg cgcgtcccct
    25861 gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac
    25921 gggcaatcat caccggtcga ccttcgtacc aacgagagtg cggatgatga tgaattttgc
    25981 gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca aacggccctt
    26041 cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg
    26101 cacgcgcttg cagcagatcc agataacgtc cgtaacccgc aagagcttcc tcaagcatcg
    26161 caatataatc ttcgccgctc tgtaaaagcc gtttttcgat atcagtaaaa tcctgctgaa
    26221 aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc
    26281 cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg
    26341 ccgcttctgt gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg
    26401 cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag
    26461 cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatcgggca
    26521 aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca
    26581 gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg
    26641 cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg
    26701 tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac
    26761 ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg
    26821 ccagcaactt cgctacggtg gtcgttttac cggtgccagg gccgccggaa atcaccgaga
    26881 tccgccgcgt cagcgccact gccgccgcaa ctttttgcca gttaatttca tcgcttactg
    26941 gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg gcatgattca
    27001 cttcgttgaa aaagcgtgcc actgtgcgct cgttacacca catgcgattc aaataaagac
    27061 gatcgccaca gaggatcatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca
    27121 gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg
    27181 gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acaaacgtgt ccctctccgg
    27241 catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct cccgccacgg
    27301 tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt
    27361 gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag
    27421 ggcaatcaac ccggcgttgg gtcgggttgt gtaaatcccc tgttgcggat gttctttatc
    27481 aacgccacgc aggaacagat aaataacgcc gccaaagtgg tgctcatagt cgtaatcagc
    27541 aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt gcagatcata
    27601 gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc
    27661 caaccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga acaccaggtc
    27721 gataaagcct tttaacatgc cacgtacctg catgaactcc agcggcgggc agcctgcgga
    27781 tagcgggtca aactggcgga ttaacgtatc aagctgactg gcgataagcg gttcactaat
    27841 cggcagataa aactccatct ccacctgttt attgcgggcg gaaagttgac tcaggcttac
    27901 gccggtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca ataccggttc
    27961 ccactgcgat tcaaagccgc cgagttccag tttttcccgc acccagttcg ggtcaaccgg
    28021 ctgggtaaaa tccaggtctt caaacaaact gtgcaagaac gtccccggtg acgcaccgcg
    28081 cggaaactga tgtggtgtta acgtcggttc ttcaacgacg ctggcaacgc ctgcagcatc
    28141 gacatccagc cgaggcatca aatcctgggc gataccgtga ccacgctgtt gcaaaccaga
    28201 gtagctggtg acgcgccagt tatcgccggg caatcgttgt aacgtcttcg cattcagctc
    28261 tgctgtagaa acatcattaa cctgccaggg ttggttatca ccagtttgtg cggtttgcca
    28321 ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg
    28381 ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt
    28441 atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgtgt
    28501 cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcggcctccg cgaggtcgac
    28561 gctttctggc gcagcattaa gatccagaac tgcctcaaac gagtggcgat cgtgataaaa
    28621 cgcctgctcc tggacgcgga aattggtgat aaacggcagc cagaccaatg gatattccag
    28681 ccctttcgat ttgtggatcg tgacaatctg caccagatgt ttatcacttt cgagacgcat
    28741 ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag
    28801 cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga tatgcaagat
    28861 atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttcagcaa tgttacgcgc
    28921 cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc
    28981 atcgaactct tcgactacca catcccacgc atgttcgtca ttgttcagcg tttcgatatc
    29041 cagcgcgttc agccccatca ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc
    29101 gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac
    29161 actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac
    29221 ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac gcaccggacg
    29281 cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg
    29341 gatttgcgca gcacatacct gcgccatggt actttgataa tcgccaacgc cgcagctttc
    29401 gccttccatc agccacattt tcatcgcagg ctgtgtttca cctttaaata caaaacgtaa
    29461 cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgc
    29521 gtcatcagtc tggctgaaaa gcttattcac gctgttcacc attcctggtg cggaacgcca
    29581 gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc gccttcatat aagtgaagat
    29641 atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt
    29701 ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg
    29761 aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcacc
    29821 gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc
    29881 acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcac
    29941 cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg
    30001 tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctggg agaatttttc
    30061 cagcgactct ggcaactgat aactgttggt ctcttcttct gcccaggcgc tgatcttgtc
    30121 gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat
    30181 cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc
    30241 cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac
    30301 cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca
    30361 ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc gacgccagaa
    30421 atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag
    30481 cataccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa
    30541 gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt
    30601 atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca
    30661 ggcgatgcgc aactcgtgga tattgctacg gatacgacca cgcaattctg ccgtggcagc
    30721 ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa aggcggcgga
    30781 accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt
    30841 gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt
    30901 ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga
    30961 cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc
    31021 cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat
    31081 cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga
    31141 agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt
    31201 gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcttctggct
    31261 tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga
    31321 aggaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc
    31381 tcattggaaa tgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga
    31441 accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt
    31501 agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttacca gctttttcaa
    31561 agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccac tctgaaccat
    31621 cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt
    31681 tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca
    31741 cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag aagtacggca
    31801 cttgcgagag catctgcgcg ggcataatcg cctgctcgaa cgctttgccc ttttctgcgg
    31861 aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc gtggcggtat
    31921 agctaaagta gccttccagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat
    31981 tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg
    32041 cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca
    32101 cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcggctt
    32161 tgggctcgct ggcaaaataa cggcttggcg catacactac tcgcagattc gactcatcaa
    32221 caatcagctc tggatggtcg tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa
    32281 tataagggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg
    32341 cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt
    32401 tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg
    32461 ccagacgttc ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg
    32521 tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac
    32581 gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt
    32641 gtttatcgat ccctttttca cgtaacaaat tgaggtagct aaaaatagcc gccacaacct
    32701 gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat acgccgctgt
    32761 tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca
    32821 gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc aattcatcgg
    32881 ttttgctacg gaatttcgcc gagttgttat caatacgaaa ctctacgcgc aacactttac
    32941 gcggcagcgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca
    33001 cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca
    33061 tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc agattggcgg
    33121 aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc
    33181 ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt
    33241 ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt
    33301 tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat
    33361 cagccaggcg gtctaccgca ccaggcaagg cgtcgttctc aacttccaga tagaaagccg
    33421 tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca
    33481 gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg
    33541 ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc agcgccgaga
    33601 gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta
    33661 tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc
    33721 atcccgtttc tgcctgactt aagggtgccc aaagggcaac taacaacaat aatgctttga
    33781 accaggtgct gcggggcatt cacggacctc ataatcaact taattttctg tccagattca
    33841 acacgttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt cagcctatgt
    33901 ttatataacc atcagtccgt gactggtgcg catcataaag taagcggata gattgcgcaa
    33961 tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac
    34021 tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagcct ttgataccag
    34081 atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgcag gaatttcgta
    34141 cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag
    34201 gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga catcccttca
    34261 cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga
    34321 aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc accgctggca
    34381 cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga
    34441 cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg
    34501 ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg
    34561 gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac
    34621 ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc
    34681 agtgcattca ataactgctg attgatttga taacggctaa gcccttccag aataaatggc
    34741 tcggtgtctg ggatttcgct gtcttcagta cggaagttca cctgcaaacg catctggaag
    34801 aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt
    34861 aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca
    34921 ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac
    34981 ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag
    35041 ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc
    35101 accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata
    35161 tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga
    35221 cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc
    35281 tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa
    35341 cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt
    35401 tcttgatcca gacgctgcgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca
    35461 tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc
    35521 gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc
    35581 aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt
    35641 agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt
    35701 agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc
    35761 aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg
    35821 tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct
    35881 tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc cagcacgtct
    35941 tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc cagtaccgga
    36001 tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg
    36061 ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg
    36121 atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg atcgtgcaaa
    36181 acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct atccagtgga
    36241 tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc agcaacggca
    36301 cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtgaca
    36361 tcgacaaagg cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg
    36421 cgcccaagct taccccatga agccagcagc gggttgccga catcctgttc accatcgctg
    36481 ttaaagagct gcccggcatt ttcgctatca cgaaatagcg gcaattcgcg atcttcaaaa
    36541 ctgtgtcgac gctgacgggt cagcagtttc gccagataag caggatcttt aatatcgccc
    36601 cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc
    36661 tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa
    36721 ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga
    36781 ttggcgcggt gccaacgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac
    36841 aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac
    36901 tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc cgcttttgag
    36961 gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta
    37021 aagtcttcgc gatccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt
    37081 ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat atcccagata
    37141 aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc
    37201 atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga
    37261 tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga
    37321 ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg gcaatgcagg
    37381 cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat
    37441 cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc
    37501 agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct gctggcgaga
    37561 ctgttcatta atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc
    37621 atcgcaacca ttacctccgg caggctaaag ccttgttgat tcttcaggga agctgacata
    37681 acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa acaatgtttc
    37741 catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca accaataagg
    37801 cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc ggttccagcg tactgcgagc
    37861 actgaactgc gggctgcgtc tgccaggagt gcattttccc ccacgccagc gccgactgaa
    37921 cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca aaactgcgat
    37981 cctgctgact cattccttgc aatagcaagc tacccaaaac cagcagcatc agaaccagag
    38041 ccagtgacga aacacctttt tcgcggttca caggttgaat cctgtcacgc tatagctggc
    38101 atccaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg tcaacaccgg
    38161 cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga tggcatccgg
    38221 attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta gcgtttccag
    38281 cacatgctcc ttcagacgaa atccaatctg gtcagactct tttaccggtt cgcgatccca
    38341 gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc cgacaatttc
    38401 cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat gcttcgcgac
    38461 ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc tcgttaaact
    38521 ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc tactgatcgc
    38581 catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca tccttctgtt
    38641 tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca ccactcgccc
    38701 gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc aaagaaagca
    38761 agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa gaccaatggt
    38821 gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca taacgtcccc
    38881 tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag
    38941 agcaaatagt cccgcgcctg gctggcggtt tgccatagcc gttgcgactg ctgccagtat
    39001 tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt
    39061 tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga ggagaaaaaa
    39121 agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg ccgttgcagg
    39181 acgttgtaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa aaaaaccgac
    39241 gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat
    39301 gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga
    39361 ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag
    39421 atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc
    39481 gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt agctggcaat
    39541 gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc aagagagttt
    39601 gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca ttttatccag
    39661 ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg
    39721 gttcagtacc gtagtgatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca
    39781 ctgtttacca tacactggtc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt
    39841 gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc
    39901 gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag
    39961 gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt
    40021 ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca
    40081 ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac
    40141 aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa
    40201 ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag
    40261 gaacaagcca gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat
    40321 aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc gcggcagcac
    40381 accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc
    40441 tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa
    40501 ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc
    40561 gataaaatca gaaacctgga agaaggaacg tttagtacgg cgggcaaaga taatcatcac
    40621 cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag
    40681 atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc
    40741 cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca
    40801 gccgctgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac
    40861 cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc
    40921 aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg
    40981 ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa
    41041 taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc
    41101 gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac
    41161 ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc
    41221 ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat
    41281 agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat
    41341 ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag
    41401 agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg
    41461 atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg
    41521 ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg
    41581 tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag
    41641 gcggcgtgct tcatcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt
    41701 gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg
    41761 atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat
    41821 gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt
    41881 gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact
    41941 ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag
    42001 tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct
    42061 ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc
    42121 actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc
    42181 gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc
    42241 catcacggta gggatcccca gcgcacgtac catgattgca gcatgggagt tggctgcgcc
    42301 atcgcgcacg acaacaccca ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga
    42361 cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc
    42421 atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag
    42481 atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc
    42541 ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc
    42601 cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc
    42661 aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg
    42721 ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa
    42781 cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat
    42841 tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa gaatagctgc
    42901 catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc
    42961 tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa
    43021 aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg
    43081 cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc
    43141 aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta
    43201 gtaacaacgt cgatcatgat cggcaaggta gaccgaacag acctcggtat ccatcgcaag
    43261 acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt
    43321 ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg
    43381 ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacgctcgc
    43441 gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg
    43501 ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt
    43561 gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca
    43621 aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg
    43681 cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg
    43741 gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga
    43801 gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc
    43861 tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata
    43921 tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac
    43981 actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg
    44041 atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac
    44101 gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta
    44161 gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac
    44221 ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca
    44281 ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg
    44341 gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag
    44401 atcctgttta ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat
    44461 taccggcagc ctaaaaagca atataaatca gagaattgaa caacacatgt ggaaaaagtt
    44521 acactgcgaa tattcggcac ataattgctg tttgtttttt aaccaaggta tcatgacatg
    44581 tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca
    44641 cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgcca
    44701 gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc
    44761 agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc
    44821 cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg
    44881 ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg
    44941 tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg
    45001 ttgctgtgga gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta
    45061 atggatatga ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta
    45121 cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc
    45181 gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta
    45241 ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat
    45301 aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc
    45361 ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca
    45421 atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc
    45481 gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct
    45541 gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg
    45601 atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg
    45661 aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta
    45721 tcgtgcagat tatgctgctg gatattatct ttagcctcga ctcggtgatt acggctgtgg
    45781 gtctgtcaga tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga
    45841 tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg
    45901 cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg
    45961 taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc
    46021 tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta
    46081 ctcactcctt ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt
    46141 ttataaacag actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc
    46201 agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt
    46261 catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga
    46321 taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga
    46381 tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt
    46441 ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa
    46501 aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg
    46561 gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc
    46621 gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct
    46681 ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat
    46741 ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac
    46801 aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac
    46861 agcctcaagc gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc
    46921 ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg
    46981 ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc
    47041 ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac
    47101 gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa
    47161 gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg
    47221 ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat
    47281 acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg
    47341 tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct cgcgtttgta
    47401 cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa
    47461 actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg
    47521 gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa accggcgtta
    47581 aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac
    47641 cgaagccaat gccaatgggc acgaccggga tgcctatcat taccgccagc gagtaaatgc
    47701 ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg
    47761 ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc
    47821 cccccatcac gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg
    47881 aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt
    47941 ccagcgtaac taacttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg
    48001 cgttgagata ggtgggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca
    48061 ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa
    48121 aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt
    48181 tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg
    48241 caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgacgagg acatgccagt
    48301 cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca
    48361 ttaaaccgtt agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    48421 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    48481 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    48541 ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac
    48601 ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta
    48661 acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga
    48721 taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg
    48781 tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    48841 agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg
    48901 tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    48961 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    49021 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    49081 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata
    49141 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    49201 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    49261 tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgcgcatc cattcctggt
    49321 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    49381 gcacattcgg tcacgccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    49441 tgtttggtac tttcttgtaa tttttctgcg cctgccacca catagcgtag acgatagaag
    49501 tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtgcc gaacaacacg
    49561 gtgcaactgc ggtcatacac caactccggc acaatacggt aatgcagcgg gcttggataa
    49621 aggaacactt ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    49681 tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    49741 atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt cggatggcct
    49801 tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc
    49861 agacgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    49921 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    49981 ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata
    50041 gcactggtca gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgg
    50101 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    50161 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    50221 aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg
    50281 aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg caccgccatt
    50341 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    50401 cgcggcgcat ccggcatcgc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg
    50461 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    50521 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag
    50581 attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    50641 ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga
    50701 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    50761 acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag
    50821 acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg
    50881 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    50941 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    51001 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    51061 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    51121 tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    51181 gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg
    51241 cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg
    51301 ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt
    51361 cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct
    51421 cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg
    51481 aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc
    51541 gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca cctaaagcca
    51601 gtgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg
    51661 ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg
    51721 tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg
    51781 gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg
    51841 agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg
    51901 ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc
    51961 ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc
    52021 gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt
    52081 ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg
    52141 ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga
    52201 ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg
    52261 cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt
    52321 taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc
    52381 gttacccaat cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata
    52441 atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag
    52501 tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc
    52561 aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca
    52621 ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg
    52681 ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc cggcaaggcg
    52741 cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc acataatggc
    52801 ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg
    52861 gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccgaca
    52921 tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac
    52981 acgccagact gcgctaccag aaagcgaccc ggttcaattt ccagtttcac agggtggccc
    53041 aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca
    53101 accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt
    53161 aaatcctgac cgaattcgat gacctgacgc accatagcac cacacacctg ttccagatgg
    53221 gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcag atgatgacgt
    53281 tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg
    53341 ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat
    53401 acccgatgcc ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc
    53461 accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta
    53521 aaaacaatat tatcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt
    53581 tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga
    53641 atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc
    53701 tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa
    53761 cgcagcagat tttcggcggt gagatcggta tcggtactga acagtgaatg tggcataaca
    53821 aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg
    53881 atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg
    53941 gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg
    54001 actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc
    54061 tttgagaagg tgatcgggct aaaattgttt gagcgcgtac gtggacgatt acatcctacc
    54121 gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt
    54181 gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg
    54241 ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc
    54301 gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc
    54361 cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc
    54421 gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct agcggtaaaa
    54481 aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac
    54541 agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc
    54601 gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg
    54661 gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc
    54721 agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg
    54781 ctggtgcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt
    54841 gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg
    54901 ccgcagtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg
    54961 tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat
    55021 aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat
    55081 taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca
    55141 gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagtagc gccacacgag
    55201 tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag
    55261 tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac
    55321 cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat
    55381 cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca
    55441 cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt
    55501 aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct
    55561 cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca
    55621 ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa
    55681 tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag
    55741 cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag
    55801 cgcagtgtag agccagaacg taccggcagc gccaatgctg tcgagcagcg tcaggaaggt
    55861 cgcgccgata atcatattcg acacccagtt cgtggtggtc gaacaggtaa taccgaaatc
    55921 gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat
    55981 cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga
    56041 agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat
    56101 cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc
    56161 aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc
    56221 tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat
    56281 accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt
    56341 acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc
    56401 gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc
    56461 ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag
    56521 aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct
    56581 ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg
    56641 gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg
    56701 agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acccgagcgg cgattaacat
    56761 ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc
    56821 catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat
    56881 tgctgcaccg agcatcatgc tactgaccac ccattcctgc aaacggctgg tcagcacaaa
    56941 gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa
    57001 tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat cccgcaaaga
    57061 acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt
    57121 aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa
    57181 atatggatta aattgctgcg gcatgtcgtt atgtgatgga tattccaatt ttcaaattaa
    57241 gttgaattat tgagattatt attaaccacc taattttaca gcagataaaa ttcataaagt
//
LOCUS       DAPPNJ010000003        51347 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000003 DAPPNJ010000000
VERSION     DAPPNJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.003"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(163..1638)
                     /gene="ampG"
                     /locus_tag="RPM73_000100"
     CDS             complement(163..1638)
                     /gene="ampG"
                     /locus_tag="RPM73_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDX6518499.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDT
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1682..2260)
                     /locus_tag="RPM73_000101"
     CDS             complement(1682..2260)
                     /locus_tag="RPM73_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6518500.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2565..2882
                     /gene="bolA"
                     /locus_tag="RPM73_000102"
     CDS             2565..2882
                     /gene="bolA"
                     /locus_tag="RPM73_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDX6518501.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTIFASPPCRGAG
                     SIA"
     gene            3226..4524
                     /gene="tig"
                     /locus_tag="RPM73_000103"
     CDS             3226..4524
                     /gene="tig"
                     /locus_tag="RPM73_000103"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDX6518502.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4770..5393
                     /gene="clpP"
                     /locus_tag="RPM73_000104"
     CDS             4770..5393
                     /gene="clpP"
                     /locus_tag="RPM73_000104"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDX6518503.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5519..6793
                     /gene="clpX"
                     /locus_tag="RPM73_000105"
     CDS             5519..6793
                     /gene="clpX"
                     /locus_tag="RPM73_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDX6518504.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6981..9335
                     /gene="lon"
                     /locus_tag="RPM73_000106"
     CDS             6981..9335
                     /gene="lon"
                     /locus_tag="RPM73_000106"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDX6518505.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9544..9816
                     /gene="hupB"
                     /locus_tag="RPM73_000107"
     CDS             9544..9816
                     /gene="hupB"
                     /locus_tag="RPM73_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDX6518506.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10008..11879
                     /gene="ppiD"
                     /locus_tag="RPM73_000108"
     CDS             10008..11879
                     /gene="ppiD"
                     /locus_tag="RPM73_000108"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDX6518507.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12030..12401
                     /locus_tag="RPM73_000109"
     CDS             12030..12401
                     /locus_tag="RPM73_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDX6518508.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12495..12893
                     /gene="fadM"
                     /locus_tag="RPM73_000110"
     CDS             12495..12893
                     /gene="fadM"
                     /locus_tag="RPM73_000110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDX6518509.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12945..13640)
                     /gene="queC"
                     /locus_tag="RPM73_000111"
     CDS             complement(12945..13640)
                     /gene="queC"
                     /locus_tag="RPM73_000111"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDX6518510.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13705..15405)
                     /locus_tag="RPM73_000112"
     CDS             complement(13705..15405)
                     /locus_tag="RPM73_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDX6518511.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15505..16323
                     /gene="cof"
                     /locus_tag="RPM73_000113"
     CDS             15505..16323
                     /gene="cof"
                     /locus_tag="RPM73_000113"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDX6518512.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16476..16934
                     /gene="decR"
                     /locus_tag="RPM73_000114"
     CDS             16476..16934
                     /gene="decR"
                     /locus_tag="RPM73_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDX6518513.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16964..18736
                     /locus_tag="RPM73_000115"
     CDS             16964..18736
                     /locus_tag="RPM73_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDX6518514.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18729..20510
                     /locus_tag="RPM73_000116"
     CDS             18729..20510
                     /locus_tag="RPM73_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDX6518515.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20691..21029
                     /gene="glnK"
                     /locus_tag="RPM73_000117"
     CDS             20691..21029
                     /gene="glnK"
                     /locus_tag="RPM73_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDX6518516.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21059..22345
                     /gene="amtB"
                     /locus_tag="RPM73_000118"
     CDS             21059..22345
                     /gene="amtB"
                     /locus_tag="RPM73_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDX6518517.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22393..23253)
                     /gene="tesB"
                     /locus_tag="RPM73_000119"
     CDS             complement(22393..23253)
                     /gene="tesB"
                     /locus_tag="RPM73_000119"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDX6518518.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23471..24043
                     /locus_tag="RPM73_000120"
     CDS             23471..24043
                     /locus_tag="RPM73_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6518519.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24074..24385)
                     /locus_tag="RPM73_000121"
     CDS             complement(24074..24385)
                     /locus_tag="RPM73_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDX6518520.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24548..24644
                     /gene="ffs"
                     /locus_tag="RPM73_000122"
     ncRNA           24548..24644
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RPM73_000122"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24764..25117
                     /locus_tag="RPM73_000123"
     CDS             24764..25117
                     /locus_tag="RPM73_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDX6518521.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25159..26709)
                     /gene="pdeB"
                     /locus_tag="RPM73_000124"
     CDS             complement(25159..26709)
                     /gene="pdeB"
                     /locus_tag="RPM73_000124"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDX6518522.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26873..27343)
                     /locus_tag="RPM73_000125"
     CDS             complement(26873..27343)
                     /locus_tag="RPM73_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDX6518523.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27459..28010)
                     /gene="maa"
                     /locus_tag="RPM73_000126"
     CDS             complement(27459..28010)
                     /gene="maa"
                     /locus_tag="RPM73_000126"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDX6518524.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28182..28400)
                     /gene="hha"
                     /locus_tag="RPM73_000127"
     CDS             complement(28182..28400)
                     /gene="hha"
                     /locus_tag="RPM73_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDX6518525.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28426..28800)
                     /gene="tomB"
                     /locus_tag="RPM73_000128"
     CDS             complement(28426..28800)
                     /gene="tomB"
                     /locus_tag="RPM73_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDX6518526.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYRDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29346..32495)
                     /locus_tag="RPM73_000129"
     CDS             complement(29346..32495)
                     /locus_tag="RPM73_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDX6518527.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32518..33711)
                     /gene="acrA"
                     /locus_tag="RPM73_000130"
     CDS             complement(32518..33711)
                     /gene="acrA"
                     /locus_tag="RPM73_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDX6518528.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33853..34500
                     /gene="acrR"
                     /locus_tag="RPM73_000131"
     CDS             33853..34500
                     /gene="acrR"
                     /locus_tag="RPM73_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDX6518529.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34628..37990
                     /gene="mscK"
                     /locus_tag="RPM73_000132"
     CDS             34628..37990
                     /gene="mscK"
                     /locus_tag="RPM73_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDX6518530.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38202..38363)
                     /gene="rsmS"
                     /locus_tag="RPM73_000133"
     CDS             complement(38202..38363)
                     /gene="rsmS"
                     /locus_tag="RPM73_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDX6518531.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38377..38904)
                     /gene="priC"
                     /locus_tag="RPM73_000134"
     CDS             complement(38377..38904)
                     /gene="priC"
                     /locus_tag="RPM73_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDX6518532.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38974..39351
                     /locus_tag="RPM73_000135"
     CDS             38974..39351
                     /locus_tag="RPM73_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDX6518533.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39504..40055
                     /gene="apt"
                     /locus_tag="RPM73_000136"
     CDS             39504..40055
                     /gene="apt"
                     /locus_tag="RPM73_000136"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDX6518534.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40184..42115
                     /gene="dnaX"
                     /locus_tag="RPM73_000137"
     CDS             40184..42115
                     /gene="dnaX"
                     /locus_tag="RPM73_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDX6518535.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41448..41512
                     /gene="dnaX"
                     /locus_tag="RPM73_000137"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42168..42497
                     /locus_tag="RPM73_000138"
     CDS             42168..42497
                     /locus_tag="RPM73_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDX6518536.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42497..43102
                     /gene="recR"
                     /locus_tag="RPM73_000139"
     CDS             42497..43102
                     /gene="recR"
                     /locus_tag="RPM73_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDX6518537.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43212..45086
                     /gene="htpG"
                     /locus_tag="RPM73_000140"
     CDS             43212..45086
                     /gene="htpG"
                     /locus_tag="RPM73_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDX6518538.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45267..45911
                     /gene="adk"
                     /locus_tag="RPM73_000141"
     CDS             45267..45911
                     /gene="adk"
                     /locus_tag="RPM73_000141"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDX6518539.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46147..47109
                     /gene="hemH"
                     /locus_tag="RPM73_000142"
     CDS             46147..47109
                     /gene="hemH"
                     /locus_tag="RPM73_000142"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDX6518540.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47106..48065)
                     /gene="aes"
                     /locus_tag="RPM73_000143"
     CDS             complement(47106..48065)
                     /gene="aes"
                     /locus_tag="RPM73_000143"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDX6518541.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48217..49521
                     /gene="gsk"
                     /locus_tag="RPM73_000144"
     CDS             48217..49521
                     /gene="gsk"
                     /locus_tag="RPM73_000144"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDX6518542.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49604..51280)
                     /locus_tag="RPM73_000145"
     CDS             complement(49604..51280)
                     /locus_tag="RPM73_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDX6518543.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT        12726 a        12905 c        13673 g        12043 t
ORIGIN      
        1 taattagctg aatcgtgtaa gaaaaataga attcataaat agcgtcaata atttccaaac
       61 gctaagccgc acaaaagaac aaatattatt aagcgatact gatgtttatt tagccgtaat
      121 aattacggcg agtgattact acagctaaat aatatttaca gattacgtca gatgcgtttt
      181 tcgtagagcc agataatcca gcaaaccacc aagcacgacg ccagaaaggg cgactaaaac
      241 cccgacttcc agcagagcag gcaggaaaga gaagtgcgtc aaatccagcg tgtccatcgt
      301 caacagcagt aaccacacgg ccaacaggct gacgcccgcc gccagtgtcc acatggcaaa
      361 ggcataacct gccggatatt cggtacggga gataaagttg tcatttactc gtgtatattc
      421 aagcgtctgg cggcaaacca gcagcaaaat aagccccggt acggcagcgg cgacggagaa
      481 tagatagaat gtcgaccagc cgtgtgcttc aacaaaccaa cccgccacgg ggccgacata
      541 aactcgtcct acagcggaaa gcgctgagag cagggcaaat tgagtagcgg aaaatgactt
      601 attacacagc gtcattaaca gcgcgacaaa ggctgatgtg cccatcccgc cacagaggtt
      661 ttcgaaaaag acggctgcgc ccatgctgta gagatgctta tcagtaatcg acagcagcca
      721 gtaaccagcg ttagacgcac cttgtaaaat gccgaaaatc agcagcgccc ggaacagcga
      781 caggcgctgc atcaaaatcc caccgtacaa tgcgccaaca atggtcgcca gtaagccaag
      841 cgttttgtta accacgccta cttcacccgc atcaaacccg acgccgcgaa tcaaaaacgt
      901 ggttgtcagg ctcatggcga atgcgtcgcc cagcttatac agcacgataa gaagcaaaat
      961 aagccaggca ttattgcgac caaagaaatc tcgcagaggt gcaacaaccg cttgttccag
     1021 cgtttttggc acaggaatgg tgtcggtcgg ttctggtgca agcaacgtcg cgataataca
     1081 ggggatcaac agcgccgcca tcaaccagta catgccctgc cagcccagcc atttatctgc
     1141 cagccacagg gctaagccgc cggaaaccag catccctaaa cggtaaccca gaacgctgat
     1201 tgccgcgccc gcaccacgtt cttctgccgg gagcacatcg gttttccacg catcgaagac
     1261 aatatcctgg gaggcagagc aaaaagcgat caccactgcc agtgccgcca tccagcggag
     1321 ttgggtgcct ggttcgagaa aacccatcgc cgcaatggcg actaataaca ggatttgcgt
     1381 ggcgagcagc caaccgcgcc gccgcccaaa aaatgggggc gtgtagcggt ccatcagcgg
     1441 tgaccagagg aatttaaaaa cgtaagcctg gcctaccaga gagaagaaac caatggtttt
     1501 gagatcgata ttctcgaccg tcatccaggc ctgtaaggtg ccggatgtca gggcgagcgg
     1561 tagcccggaa gcaaaaccca ggatcagcaa tatggctgaa cgcggctgtt gaaaaatacg
     1621 taaatattga ctggacatgg gcgtctacag gcccggcttg caccgggcca gagggcagaa
     1681 attaacgcgc gttctgcttg atgaattcgt ggatgctggt gtcctgagac atatcagcga
     1741 tggtatcggt cagcacactg ttaaccgcat cggcgatatt tttgttggag gcctggaacg
     1801 caccttcaac gttgtagctg gcacgatagt ttttggtcat tttgttgcca ttctgcgcgg
     1861 tagcgatgat ggcgatatcc gctttggtag cgatgttgta gcgcacgttg ccctgggaca
     1921 cgtcagcata cagttggcta acgatgattt gcagattaac cgggccattc ggaccaacca
     1981 tgtaaccacg cgcggtcatc tgtttttcca gcacttcttg cagcaggaaa cgcagatcgc
     2041 gggaggcggt cagggtaacg atttgattat cgcgggtgac ttttgccagc gcctgatcgg
     2101 tacgctgatc ggcaccatta atgcttacgg tgacgcccat caggcttgga tcctgctgtg
     2161 gcagtgtaat cgtcggggaa acttcaatag ttgttggcgg ttttgcgcat cctgcaagca
     2221 taaacagagc aactaacggg aagaggattt ttttgaacat gttcgggctc tcagagactc
     2281 ttaagcgtgt ttggtaaaaa ttcccgccat cataacattg ccaacggcga ggggaagtgg
     2341 gtaaggcatg taaattcatc atgttgacga aataatcgcc cctggcaaaa gaaacactga
     2401 tgcgaggcct gtgtttcaat ctttaaatca gtaaacttca tacgcttgac ggaaaaacca
     2461 ggacgaaacc taaatatttg ttgttaagct gcaatggaaa cggtaaaagc ggctagtatt
     2521 taaagggatg gatgacatct cagcgttgtc ggaggagata tttcatgatg atacgtgagc
     2581 ggatagaaga aaaattaagg gcggcgttcc aacccgtatt cctcgaagta gtggatgaaa
     2641 gctatcgtca caatgtccca gccggctctg aaagccattt taaagttgtg ctggtcagcg
     2701 atcgttttac gggtgaacgt tttctgaatc gtcatcgaat gatttacagt actttagcgg
     2761 aggaactctc tactaccgtt catgcgctgg ctctgcatac ttacactatt aaggagtggg
     2821 aagggttgca ggacaccatc tttgcctctc ctccctgtcg tggagcagga agcatcgcgt
     2881 aaaaacgcat ttgcaactgt cggcgctttt ccagtatgtt gctaaagatt ttatgaaaaa
     2941 cggcctgcgg gccgttttgt tttgtctgga ttttgcgctt tttgcccagc attcagacga
     3001 aaattgcccg ggaattgtga aaaaatacgc gacagcgcgc aataaccgtt ctcgactcat
     3061 aaaagtgatg ccgctataat gccgcgtcct atttgaatgc tttcgggatg attctggtaa
     3121 cagggaatgt gattgattat aagaacatcc cggttccgcg aagccaacaa cctgtgcttg
     3181 cggggtaaga gttgaccgag cactgtgatt ttttgaggta acaagatgca agtttcagtt
     3241 gaaaccactc aaggccttgg ccgccgtgta acgattacta tcgctgctga cagcatcgag
     3301 accgctgtta aaagcgagct ggtcaacgtt gcgaaaaaag tacgtattga cggcttccgc
     3361 aaaggcaaag tgccaatgaa tatcgttgct cagcgttatg gcgcgtctgt acgccaggac
     3421 gttctgggtg acctgatgag ccgtaacttc attgacgcca tcattaaaga aaaaatcaat
     3481 ccggctggcg caccgactta tgttccgggc gaatacaagc tgggtgaaga cttcacttac
     3541 tctgtagagt ttgaagttta tccggaagtt gaactgcagg gtctggaagc gatcgaagtt
     3601 gaaaaaccga tcgttgaagt gaccgacgct gacgttgacg gcatgctgga tactctgcgt
     3661 aaacagcagg cgacctggaa agaaaaagac ggcgctgttg aagcagaaga ccgcgtaacc
     3721 atcgacttca ccggttctgt agacggcgaa gagttcgaag gcggtaaagc gtctgatttc
     3781 gtactggcga tgggccaggg tcgtatgatc ccgggctttg aagacggtat caaaggccac
     3841 aaagctggcg aagagttcac catcgacgtg accttcccgg aagaatacca cgcagaaaac
     3901 ctgaaaggta aagcagcgaa attcgctatc aacctgaaga aagttgaaga gcgtgaactg
     3961 ccggaactga ctgcagaatt catcaaacgt ttcggcgttg aagatggttc cgtagaaggt
     4021 ctgcgcgctg aagtgcgtaa aaacatggag cgcgagctga agagcgccat ccgtaaccgc
     4081 gttaagtctc aggcgatcga aggtctggta aaagctaacg acatcgacgt accggctgcg
     4141 ctgattgaca gcgaaatcga cgttctgcgt cgtcaggctg cacagcgttt cggtggcaac
     4201 gaaaaacaag ctctggaact gccgcgcgaa ctgttcgaag aacaggctaa acgccgcgta
     4261 gttgttggcc tgctgctggg cgaagttatc cgcaccaacg agctgaaagc tgacgaagag
     4321 cgcgtgaaag gcctgatcga agagatggct tctgcgtacg aagatccgaa agaagttatc
     4381 gagttctaca gcaaaaacaa agaactgatg gacaacatgc gcaatgttgc tctggaagaa
     4441 caggctgttg aagctgtact ggcgaaagcg aaagtgactg aaaaagaaac cactttcaac
     4501 gagctgatga accagcaggc gtaatttacg cagcataacg cgctaaattc gcacaaaggc
     4561 ccgtcaccgc caggtggtgg gctttttttt gtcatgaatt ttgcatggaa ccgtgcgaaa
     4621 agcctctttc ggtgttagcg taacaacaaa agattgttat gcttgaaata tggtgatgcc
     4681 gtacccataa cacagggact agctgataat ccgtccataa ggttacaatc ggtacagcag
     4741 gttttttcaa ttttatccag gagacggaaa tgtcatacag cggcgaacga gataactttg
     4801 caccccatat ggcgctggtg ccgatggtca ttgaacagac ctcacgcggt gagcgctctt
     4861 ttgatatcta ttctcgtcta cttaaggaac gcgtcatttt tctgactggc caggttgaag
     4921 accacatggc taacctgatt gtggcgcaga tgctgttcct ggaagcggaa aacccagaaa
     4981 aagatatcta tctgtacatt aactccccag gcggggtgat cactgccggg atgtctatct
     5041 atgacaccat gcagtttatc aagcctgatg tcagcaccat ctgtatgggc caggcggcct
     5101 cgatgggcgc tttcttgttg accgcagggg caaaaggtaa acgtttttgc ctgccgaatt
     5161 cgcgcgtgat gattcaccaa ccgttgggcg gctaccaggg ccaggcgacc gatatcgaaa
     5221 ttcatgcccg tgaaattctg aaagttaaag ggcgcatgaa tgaacttatg gcgcttcata
     5281 cgggtcaatc attagaacag attgaacgtg ataccgagcg cgatcgcttc ctttccgccc
     5341 ctgaagcggt ggaatacggt ctggtcgatt cgattctgac ccatcgtaat tgatgccaga
     5401 ggcgcaactg tgccgctata cttatccagg gcggcacaac gctgtaagcg gcttgcgcct
     5461 gagaatggca tttgcgtcgt cgtgtgcggc acaaagaaca aagaagaggt tttgacccat
     5521 gacagataaa cgcaaagatg gctcaggcaa attgctgtat tgctcttttt gcggcaaaag
     5581 ccagcatgaa gtgcgcaagc tgattgccgg tccatccgtg tatatctgcg acgaatgtgt
     5641 tgatttatgt aacgacatca ttcgcgaaga gattaaagaa gttgcaccgc atcgtgaacg
     5701 cagtgcgcta ccgacgccgc atgaaattcg caaccacctg gacgattacg ttatcggcca
     5761 ggaacaggcg aaaaaagtgc tggcggtcgc ggtatacaac cattacaaac gtctgcgcaa
     5821 cggcgatacc agcaatggtg tcgagttggg caaaagtaac attctgctga tcggtccgac
     5881 cggttccggt aaaacgctgc tggctgaaac gctggcgcgc ctgctggacg tcccgttcac
     5941 catggccgac gcaaccacgc tgaccgaagc cggttatgtg ggcgaagacg ttgaaaacat
     6001 cattcagaag ctgttgcaga agtgcgatta cgacgtacag aaagcgcagc gcgggattgt
     6061 ctacatcgat gaaatcgaca agatttctcg taagtcagac aacccgtcca ttacccgaga
     6121 cgtttccggt gaaggcgtac agcaggcact gttgaaactg atcgaaggta cggtagctgc
     6181 tgttccaccg caaggtgggc gtaaacatcc gcagcaggaa ttcttgcagg ttgatacctc
     6241 taagatcctg tttatttgtg gcggtgcgtt tgccggtctg gataaagtga tttcccaccg
     6301 tgtagaaacc ggctccggca ttggttttgg cgcgacggta aaagcgaagt ccgacaaagc
     6361 aagcgaaggc gagctgctgg cgcaggttga accggaagat ctgatcaagt ttggtcttat
     6421 ccctgagttt attggtcgtc tgccggttgt cgcaacgttg aatgaactga gcgaagaagc
     6481 tctgattcag atcctcaaag agccgaaaaa cgccctgacc aagcagtatc aggcgctgtt
     6541 taatctggaa ggcgtggatc tggaattccg tgacgaggcg ctggatgcta tcgctaagaa
     6601 agcgatggcg cgtaaaaccg gtgcccgtgg cctgcgttcc atcgtagaag ccgcactgct
     6661 cgataccatg tacgatctgc cgtccatgga agatgtcgaa aaagtggtta tcgacgagtc
     6721 ggtaattgat ggtcaaagca aaccgttgct gatttatggt aagccggaag cgcaacaggc
     6781 atctggtgaa taattaacca ttcccataca attagttaac caaaaagggg ggattttatc
     6841 tcccctttaa tttttcctct attctcggcg ttgaatgtgg gggaaacatc cccatatact
     6901 gacgtacatg ttaatagatg gcgtgaagca cagtcgtgtc atctgattac ctggcggaaa
     6961 ttaaactaag agagagctct atgaatcctg agcgttctga acgcattgaa atccccgtat
     7021 tgccgctgcg cgatgtggtg gtttatccgc acatggtcat ccccttattt gtcgggcggg
     7081 aaaaatctat ccgttgtctg gaagcggcga tggaccatga taaaaaaatt atgctggtcg
     7141 cgcagaaaga agcttcaacg gatgagccgg gtgtaaacga tcttttcacc gtcgggaccg
     7201 tggcctctat attgcagatg ctgaaactgc ctgacggcac cgtcaaagtg ctggtcgagg
     7261 ggttacagcg cgcgcgtatt tctgcgctct ctgacaatgg cgaacacttt tctgcgaagg
     7321 cggagtatct ggagtcgccg accattgatg agcgggaaca ggaagtgctg gtgcgtactg
     7381 caatcagcca gttcgaaggc tacatcaagc tgaacaaaaa aatcccacca gaagtgctga
     7441 cgtcgctgaa tagcatcgac gatccggcgc gtctggcgga taccattgct gcacatatgc
     7501 cgctgaaact ggctgacaaa cagtccgttc tggagatgtc cgacgttaac gaacgtctgg
     7561 aatatctgat ggcaatgatg gaatcggaaa tcgatctgct gcaggttgag aaacgcattc
     7621 gcaaccgcgt taaaaagcag atggagaaat cccagcgtga gtactatctg aacgagcaaa
     7681 tgaaagctat tcagaaagaa ctcggtgaaa tggacgacgc gccggacgaa aacgaagccc
     7741 tgaagcgcaa aatcgacgcg gcgaagatgc cgaaagaggc aaaagagaaa gcggaagcag
     7801 agttgcagaa gctgaaaatg atgtctccga tgtcggcaga agcgaccgta gtgcgtggtt
     7861 atatcgactg gatggtacag gtaccgtgga atgcgcgcag caaggtcaaa aaagacctgc
     7921 gtcaggcgca ggaaatcctt gataccgacc attatggtct ggagcgcgtg aaagatcgca
     7981 tccttgagta ccttgcggtt caaagccgtg tcaacaaaat caagggaccg atcctctgcc
     8041 tggtagggcc gccgggggta ggtaaaacct ctcttggtca gtccattgcc aaagccaccg
     8101 ggcgtaaata tgtccgtatg gcgctgggcg gcgtgcgtga tgaagcggaa atccgtggtc
     8161 accgccgtac ttacatcggt tctatgccgg gtaaactgat ccagaaaatg gcgaaagtgg
     8221 gcgtgaaaaa cccgctgttc ctgctcgatg agatcgacaa aatgtcttct gacatgcgag
     8281 gcgatccggc ctctgcactg cttgaagtgc tggatccaga gcagaacgta gcgttcagcg
     8341 accactacct ggaagtggat tacgatctca gcgacgtgat gtttgtcgcg acgtcgaact
     8401 ccatgaacat tccggcaccg ctgctggatc gtatggaagt gattcgcctc tccggttata
     8461 ccgaagatga aaaactgaac atcgccaaac gtcacctgct gccgaagcag attgaacgta
     8521 atgcactgaa aaaaggtgag ctgaccgtcg acgatagcgc cattatcggc attattcgtt
     8581 actacacccg tgaggcgggc gtgcgtggtc tggagcgtga aatctccaaa ctgtgccgca
     8641 aagcggttaa gcagttactg ctcgataagt cattaaaaca tatcgaaatt aacggcgata
     8701 acctgcatga ctacctcggt gttcagcgtt tcgactatgg tcgcgctgat aacgaaaacc
     8761 gtgtcggtca ggtaaccggt ctggcgtgga cggaagtggg cggtgacttg ctgaccattg
     8821 aaaccgcatg tgttccgggt aaaggcaaac tgacctatac cggttcgctc ggcgaagtga
     8881 tgcaggagtc tattcaggcg gcgttaacgg tggttcgtgc gcgtgcggaa aaactgggga
     8941 tcaaccctga tttttacgaa aaacgtgaca tccacgtcca cgtaccggaa ggtgcgacgc
     9001 cgaaagatgg tccgagtgcc ggtattgcta tgtgcaccgc gctggtttct tgcctgaccg
     9061 gtaacccggt tcgtgccgat gtggcaatga ccggtgagat cactctgcgt ggtcaggtac
     9121 tgccgatcgg tggtttgaaa gaaaaactcc tggcagcgca tcgcggcggg attaaaacag
     9181 tgctaattcc gttcgaaaat aaacgcgatc tggaagagat tcctgacaac gtaattgccg
     9241 atctggacat tcatcctgtg aagcgcattg aggaagttct gactctggcg ctgcaaaatg
     9301 aaccgtctgg catgcaggtt gtgactgcaa aatagtgacc tcgcgcaaaa tgcactaata
     9361 aaaacagggc tggcaggcta attcgggctt gccagccttt ttttgtctcg ctaagttaga
     9421 tggcggatcg ggcttgccct tattaagggg tgttgtaagg ggatggctgg cctgatataa
     9481 ctgctgcgcg ttcgtacctt gaaggattca agtgcgatat aaattataaa gaggaagaga
     9541 agagtgaata aatctcaatt gatcgacaag attgctgcag gggctgatat ctctaaagct
     9601 gcggctggcc gtgcgttaga tgctattatt gcttccgtaa ctgaatctct gaaagaaggg
     9661 gatgatgtag cactggtagg ttttggtact tttgccgtta aagagcgtgc tgcccgtact
     9721 ggccgcaacc cgcagaccgg taaagagatc accatcgctg ctgctaaagt accgagcttc
     9781 cgtgcaggta aagcactgaa agacgcggta aactaagcgt tgtccccggt ggggatgtga
     9841 cgaagttcaa gggcgcatct actgatgtgc cttttttatt tgtattcggt gactttctgc
     9901 gtcttgtggg ctgacaattg cccccgtttc ttgtcacaat aggcctttgc gcgcatcgat
     9961 acgttgcgtg aggtacacag tcatctacag cggagtgttg ttacaccatg atggacagct
    10021 tacgcacggc tgcaaacagt ctcgtgctca agattatttt cggtatcatt atcgtgtcgt
    10081 tcatattgac cggcgtgagt ggttacctga ttggcggagg caataactac gccgcaaaag
    10141 tgaatgacca ggaaatcagc cgtgggcagt tcgagaacgc ctttaacagc gagcgtaatc
    10201 gcatgcagca acagctgggc gatcaatact ccgagctggc agcgaatgaa ggctatatga
    10261 aaaccctgcg tcaacaggtg ctgaatcgtc tgatcgacga ggcgctgttg gatcagtacg
    10321 ctcgtgagct gaaactgggt atcagtgatg agcaggttaa acaggcgatt ttcgcgaccc
    10381 cagccttcca ggttgacggc aaatttgata acagccgcta taacggtatc ctcaaccaga
    10441 tggggatgac cgccgatcag tacgcccagg cgctgcgtaa ccagctcact acccaacagc
    10501 tgattaacgg tgttgccggt accgatttta tgctgaaagg tgaaaccgac gagctggcgg
    10561 cactggtcgc gcaacaacgc gtggtgcgtg aggcgactat cgatgttaac gcgctggcgg
    10621 cgaagcagcc tgtgaccgaa caggagattg ccagctacta cgaacaaaac aaaaacaatt
    10681 tcatgacgcc ggaacaattc cgcgtgagtt acatcaagct ggatgccgca acgatgcagc
    10741 aaccggttag cgatgcggat atccagagct actacgatca gcatcaggat caattcaccc
    10801 agccgcagcg tacccgctac agcatcatcc agaccaaaac tgaagatgaa gcgaaagcgg
    10861 tacttgatga gctgaataaa ggcggtgatt ttgctgcatt agccaaagaa aaatctgccg
    10921 atattatctc tgcccgtaac ggcggcgata tgggttggtt agaagatgcc actatccccg
    10981 acgaactgaa aaatgctggt ctgaaagaaa aagggcaact gtctggtgtc atcaaatctt
    11041 cggtcggttt cctgattgta cgtctggacg acattcagcc agcgaaagtg aaatcgttag
    11101 acgaagtacg tgacgacatt gcggcgaaag tgaaacacga aaaagccctc gatgcgtact
    11161 atgcgctgca gcagaaagtg agcgatgcgg caagcaacga caccgagtct ctggccggtg
    11221 cagagcaagc tgccggcgtt aaagccactc agacgggttg gtttagcaaa gataacctgc
    11281 cggaagagtt gaacttcaag ccggttgccg acgctatctt taacggcggt ctggtaggtg
    11341 aaaacggcgc gccgggcatc aactctgaca tcatcaccgt agacggcgac cgcgcattcg
    11401 tgctgcgcat cagcgagcac aaaccggaag cggtgaaacc gttggcagat gttcaggaac
    11461 aagttaaggc actggttcag cacaacaaag ctgaacaaca ggcgaaagtg gatgctgaga
    11521 aactgctggt tgatttgaaa gccggcaaag gtgcggaagc tatgcaggct gccggtctga
    11581 aatttggcga gccgaaaatc ttaagccgtt ccggtcgtga cccgattagc caggcggcgt
    11641 ttgcactgcc actgccagcg aaagacaaac cgagctacgg tatggcgacc gatatgcaag
    11701 gtaatgtggt tctgctggcg ctggatgaag tgaaacaagg ttcaatgccg gaagatcaga
    11761 aaaaagcgat ggtgcagggt atcacccaga acaacgcaca aatcgtcttt gaagctctga
    11821 tgagtaacct gcgtaaagag gcgaaaatca aaattggcga tgcgctggaa cagcaataat
    11881 cctgaagccg cctcgcaaaa aaatgcgttg cagctgtaac aagaaaaggt cgctttcgcg
    11941 gccttttcca tttctgaaca ttgccatttg tttactgttt tcactcccgt taaggtgatt
    12001 ccactgttaa caaacaagga gaaaacagta tgaaacacgg aattaaagca ctgctcatta
    12061 ccctgtccct ggcctgtgcc ggaatgtctc atagcgcgct ggcggcagct tctgtggcga
    12121 aaccgacggc ggtagaaacc aaagcggaag ctcctgcagc acaaagtaaa gcagcagtac
    12181 cggcgaaagc cagtgacgaa gaaggcaccc gggtcagcat taataatgcc agcgcggaag
    12241 agctagcccg cgcgatgaat ggcgttggcc tgaagaaagc gcaggcgatt gtcagttatc
    12301 gcgaagagta cggtccgttt aaaacggtgg aggatctaaa gcaggtgccg gggatgggca
    12361 attcgctggt ggaacgtaat ctggcggtat taaccctgta attaatttgc atagtggcaa
    12421 ttttttgcca gactgaagag gtcataccag ttatgacctc tgtacttata acaacaacgt
    12481 aaggttattg cgctatgcaa acacaaatca aagttcgtgg atatcatctc gacgtttacc
    12541 agcacgtcaa caacgcccgc taccttgaat ttctcgaaga agcccgctgg gatgggttgg
    12601 aaaatagcga cagttttcag tggatgacgg cccataacat cgccttcgtc gtggtcaata
    12661 tcaatattaa ctatcgtcgc ccagcggtat taagcgacct gttaactatt accagtcagt
    12721 tgcagcaatt aaacggtaaa agcggcatct taagccaggt cattacactg gagccggaag
    12781 ggcaggtggt agcggatgcg cttattacgt ttgtttgtat tgatcttaaa acgcagaaag
    12841 cattagctct ggaaggggaa ttgcgcgaaa agctggagca gatggttaag taaacgtttt
    12901 gtggtgccgg atgctcaagc cgcatccggc gacacccgga ataattacct caacccggtt
    12961 ttctgcttca tcgctgccat caccgtcggt ttatcggcca gataatgatt caaaccgttg
    13021 gcgcgtaaat tacatgccgc acaatgaccg caaccgtcgc ctttaatgcc gttatagcag
    13081 gtcaacgttt cgttacggac taaatccagt ttgccgtaat aatctgccag cgcccaggtt
    13141 tccgctttat caatccacat cagcggcgtt tcaaaacgaa tatctttcgc catgcccaaa
    13201 ctgacggcat ggtttagtgc tttcacaaac tcatcgcggc aatccgggta gccggagaaa
    13261 tccgtttcac agacgccggt aattacggct tctgctttta cctgatacgc atatattgcc
    13321 gccagcgtca ggaacaaaat attacgccct gggacaaacg tattcgggat gccatcggct
    13381 tcaggttcat aatcaggcac cggaatgctg tcacgtgtca ggctgctgac cgccagctcg
    13441 ttgagcagcg ttacatcaag aaccttatgc gcgcgtgctc ccagtttcag cgccagttcg
    13501 cgtgccacgt cgatttctgc gcgatgtcgc tgaccgtaat cgaacgtcac gcaatggact
    13561 tcatcatatt gttgtaatgc ctgcaccaga caggtggtgg aatcctggcc tccactgaac
    13621 acaacgacag cacgtttcat agataatcct gcttgaacaa taaaagcgtt atggtaacgc
    13681 ctgcgattaa cccggaccag ctattcattg cgacggcgcg ggaagccagg cttcggtaaa
    13741 ttcaaaccag ccgcgcggtg tcagtcgcac accgttcacg ccgggagggg cactaatgcg
    13801 atagtgatag ttgaacagcg gcgtcagcgt cgcgtctgcc attaactggc taaaaaccgc
    13861 tttcagggca ttaaatcggt tttcctcatc aggcattact tgcaccgcgt ccagcgtcga
    13921 ttgcagatgt gcatatgctg gagcgtcgaa aacatgtggc catagtggat cgcaacgtag
    13981 ccattgctcc agagtatatt ccggtgcttc gccaattaat ctgtcgccca tcatgaggtc
    14041 tgcgtgtgcc agtagggtcg tgtcgtccca gttttttgcg ttatgaaaaa taattgtgag
    14101 ttcacagcct tccgctgcca gtgtcgcctg taggcgttct gccatggtat gaagttctat
    14161 cggtaagtga taaaccagcg tcaaggtttt cggtagtttg acttcatccg gtacttgcca
    14221 gtgcggaata gtccagcctg gcagtaatgc atgactggcg gtgatcaggt tttctccgac
    14281 ttctaacgtt tgtaataaac cggattgatg aataatggag atcacttttc gcgcctgcca
    14341 gagggagagt cggggacttt tgcgcaacgt taaatagcaa aaacctaaac tgatgccact
    14401 actgacctgg ctgacccgtt gcagctcctc cggtttgccg atggtgattt gcacgggatg
    14461 ccgacaactg gttcccaaat ctttttcgaa aagcggcgga gttatccagt actcaaccgc
    14521 tttaagcagc ggatgacgca aatggtaata atcatggctc tccaggcgca ccagctccgc
    14581 tgtgaattgt gttaagcgaa aaggaccagt gccgatcagt gggaattgcg gatgcgccag
    14641 atggctgcaa tagctcgcca gccggtgcgc aagccagtaa tcggggcgat gtaaaaagaa
    14701 ggtcagacac tgcggatggg tgacttcaat acgcttcacg ctaataaata attgatccag
    14761 tgctggcagt tgtaacagca tcaataatcg ctggtgtaag tgtgaggctt ttactgcatc
    14821 gccgttatgc cagtgtaggg ttgaacgaag ataaaagtcc cagcgtaacc cgtcagtaga
    14881 ggtttcccag tgatgcgcta aatcgccaat cgggcgctga gtattattat cgaagcgggt
    14941 caggccggaa aatatctgcc cggcgagatg ctgctcggca cggccgggca aaaagcctgg
    15001 ttgtagcggt tcgagcgggc gatagtaggg aatacgcaac gtgggtgtat cgttttgcca
    15061 ttgtccgccc ataaacggct gtaacagagt gcgcagctca cctggggcca gttgcgccag
    15121 ctccagcaca tcttgctgct ttccggtttc cagtgcctgt tccatcatcg cattgcgtag
    15181 cgattccggc gtgaccagaa agcgtaattg tccgcgcttt ccgcgtcctg actgcgcctg
    15241 ccactccagc catcccgcct cctgtgcctg acgcaacagc gtacgaacat ggcgttcgct
    15301 gcaaaaacag cgttcggcca gttcgctgac ggtgacggtt tgcggctttc cggctgaagg
    15361 ttgccacaga cgttgatact ggttaagacg gttgagcaat cgcatataaa cccggaacaa
    15421 tattatttaa ctattcacta ttacttccgt atatatcagg tgatactcaa tcaccattaa
    15481 ccgtgtcaca gagtggagaa agaaatggct cgtctggcag catttgatat ggatggcact
    15541 ttattgatgc ctgaccatca tttaggtgag aaaaccctct ctactttggc gcgactgcgt
    15601 gaacgcgaca ttaccctcac ttttgccacg gggcgtcatg cgctggagat gcagcatatt
    15661 ctcggggcgc tatcgctgga tgcatatttg attaccggca acggaacgcg cgtgcattct
    15721 ctggaaggtg aacttttaca tcgtgatgat ttacctgcgg atgtcgcgga gctggtgctg
    15781 tatcagcaat gggatacccg agccagcatg catatcttca atgacgacgg ttggtttacc
    15841 gggaaagaga tccctgcgtt gttgcaggca tttgtctata gcggttttcg ttatcagata
    15901 atcgatgtca aaaaaatgcc actcggcagc gtcaccaaga tctgcttctg tggcgatcac
    15961 gacgatctta cacgcttgca gatccagcta tacgaagcat taggcgagcg tgcacatttg
    16021 tgtttttccg ccacggattg cctcgaagtg ctgccggtgg gctgcaataa aggcgctgca
    16081 ttgacggtgc tgacccaaca tttaggttta tcgttgcgcg attgcatggc ctttggtgat
    16141 gcgatgaacg atcgcgaaat gttaggcagc gtcggtagcg gatttattat gggcaatgcg
    16201 atgccgcaac tgcgcgcgga gctcccgcat ttaccggtga ttggacattg ccgaaatcag
    16261 gctgtctctc actatttgac gcactggctg gactatccac atctacctta ttcccccgaa
    16321 taacgagatc ccttccagca ccgggcaatt gcccggtttt ttttgcgttg agtttgtcat
    16381 tttgtgccat ggtgtttaaa ccgcacagaa taaattgtcg cgatttcacc tttaaaatag
    16441 aattaaaaga gaaaaaaatt ctctgtggaa gggctatgtt agataaaatt gaccgtaagc
    16501 tgctggcctt actacagcag gattgcaccc tctctttgca ggcactggct gaagccgtta
    16561 atctgacaac caccccctgc tggaagcgcc tgaaacggct ggaggacgac ggtatcctta
    16621 tcggcaaagt cgccctgctg gatccggaaa aaataggcct cggcctgacc gcctttgtgc
    16681 tgataaaaac gcaacatcac agcagcgaat ggtattgccg ctttgtcacg gtggttaccg
    16741 aaatgccaga agtgctgggg ttctggcgca tggctggtga atacgattat ctgatgcgcg
    16801 tccaggttgc cgacatgaaa cgctacgacg agttttataa gcgtctggta aacagcgtgc
    16861 cggggctgtc ggacgtcact tccagcttcg cgatggaaca gattaaatac accacttctt
    16921 tacccatcga ataaatatcc agaatcaggt caggacacaa cgcgtgcgat tatttgctca
    16981 attaagctgg tatttccgtc gggaatggcg tcgctatctc ggggctgtcg ccttgcttgt
    17041 cattatcgcg atgctgcaac tggttccgcc aaaagtggtt ggtattgttg tcgatggcgt
    17101 gacagaacaa cactttacta ccgggcagat cctgatgtgg atcgccacca tggtgctgat
    17161 tgccgttgtg gtttatctcc tgcgttacgt ctggcgcgta ttgctgtttg gtgcgtctta
    17221 tcaactggct gttgaactgc gtgaagatta ttaccgtcag ctaagccggc agcatcctga
    17281 gttttacctg cgtcatcgca cgggtgacct catggctcgt gcgactaatg acgtcgatcg
    17341 cgtcgtgttt gccgccggag aaggggtgct gacgctggtg gattcactgg taatgggctg
    17401 cgctgtgttg attatgatgt ctacgcaaat tagctggcag ttgaccttat ttgccctgtt
    17461 accgatgcca gtgatggcga tcatgattaa gcgcaacggc gatgccttgc atgaacgctt
    17521 taagctggca caggcggcgt tttctagtct taatgaccgc acccaggaaa gcctcaccag
    17581 tattcgcatg atcaaagcct ttggtctgga agatcgccag tcggcgttat ttgccgcgga
    17641 tgccgaagat accggcaaaa aaaacatgcg ggtggcgcgt attgatgctc gtttcgaccc
    17701 gaccatctat atcgcgattg gtatggcgaa cttgctggcg attggcggcg gtagttggat
    17761 ggtggtgcag ggcagtttaa cgctgggcca gctcaccagt tttatgatgt atttaggtct
    17821 gatgatttgg ccaatgctgg cgctggcatg gatgtttaac attgtggaac gtggtagtgc
    17881 tgcgtacagc cgtattcgcg cgatgctggc ggaagcgccg gtggtgaacg atggtagcga
    17941 acccgtgccg gaagggcgtg gcgaactgga tgtaaatatt caccagttca cgtatccgca
    18001 gactgaccat cctgcgctgg aaaacgtcaa tttcgccctg aaacccggtc agatgctggg
    18061 tatttgcggg ccgactggtt ccggcaaaag taccctgttg tcgctcattc agcgtcattt
    18121 cgacgtcagc gagggggata ttcgctttca tgatattcct ctgacgaagt tacaactcga
    18181 tagctggcgt agccgcctgg cggtagttag ccagacaccg ttcctttttt ccgacaccgt
    18241 agcgaacaac atcgcgttgg gttgcccgaa tgccacccag caagagattg agcatgtcgc
    18301 gcggttagcc agcgtacatg acgatatttt gcgtttaccg caaggttacg atacagaggt
    18361 gggcgagcgc ggcgtgatgc tttccggcgg gcaaaaacag cgtatctcta ttgctcgtgc
    18421 gttattagtc aacgcggaaa tcctcatcct tgatgatgcg ctttcggcgg tggacggacg
    18481 cacagagcac cagatcctgc ataacctgcg tcagtgggga cagggtagaa cggtaatcat
    18541 cagtgcccat cgcctttcgg cactgacgga agccagtgaa attattgtga tgcagcacgg
    18601 acatatcgcc cagcgtggca atcatgatga gctggcgcaa caaagcggct ggtatcgcga
    18661 tatgtatcgc tatcaacaac tggaggcggc gctcgacgac gctccggaaa ttcgcgagga
    18721 ggccgtcgat gcgtagtttt agccaactgt ggccgactct caagcgtctg ttagcgtacg
    18781 gttcgccgtg gcgtaaaccg ctggggattg cggtcctgat gatgtgggtt gcggcagcgg
    18841 cagaagtcag tgggccgctg cttatcagct attttatcga caatatggta gcgaaaaata
    18901 acctgccgtt gaaaatggtt gcagggctgg ctgcggcgta tgttgggcta caactgtttg
    18961 ccgccgggct acattacgcg cagtcgctgc tgtttaatcg ggcggcagta ggcgtagtgc
    19021 aacagttgcg taccgacgtg atggatgccg cattgcgcca gccattaagc gagtttgata
    19081 cccaacccgt cgggcaggtg atttcccgcg tcactaatga cactgaagtg atccgcgatc
    19141 tctacgttac cgtagtggca actgtcctgc gtagcgccgc actggtgggc gcgatgctgg
    19201 tggcgatgtt cagcctcgac tggcgaatgg cgctggtggc gataatgatt ttcccggtgg
    19261 tgctggtggt aatggtgata taccagcgtt acagcacgcc gattgtccgt cgtgtgcgcg
    19321 cctatctagc ggatatcaac gacggcttta acgaaatcat caatggcatg agcgttatcc
    19381 agcagttccg tcagcaggcg cgatttggcg aacgtatggg ggaggccagt cgttcacact
    19441 atatggccag gatgcaaacc ctgcgcctcg acggttttct gctgcgtccg ctgctgagtc
    19501 tgttttcatc gctcattctt tgtggcttgt tgatgctgtt tggcttctcc gctagcggca
    19561 ccattgaagt gggcgtgctg tatgcgttta tcagctatct tgggcgactt aacgaaccat
    19621 taatcgaact gaccacgcaa caggcgatgc tgcaacaggc tgttgttgct ggtgagcgcg
    19681 tgtttgaact gatggacgga ccgcgccagc aatatggcaa tgatgagcgc ccgttacaga
    19741 gtggcaccat cgaagtcgat aacgtgtcat ttgcttatcg cgatgacaat ctggtgctaa
    19801 agaacattaa tctctctgtg ccttcgcgta actttgtggc actggtcggg cataccggca
    19861 gtggcaaaag caccctcgcc agtttattga tgggctatta cccgctaacg gaaggtgaga
    19921 ttcgccttga tggtcgtcca ttaagttcgc taagtcacag cgcgctgcgc cagggcgtgg
    19981 caatggtgca gcaagatccg gtggtgctgg cggatacctt cctcgccaac gtgacgctgg
    20041 ggcgtgatat ctccgaagaa cgcgtctggc aggcgctgga aaccgtgcaa ctggcggagc
    20101 tggcgcgcag catgagtgat ggtatttata cgccgctggg cgagcagggg aataatctct
    20161 cagttgggca aaagcaactg ctggcactgg cacgcgtgtt ggtggagacg ccgcaaatcc
    20221 tgatccttga tgaggcaacc gccagcattg actccggtac tgaacaggcg attcaacatg
    20281 ctctggcggc tgtgcgtgaa cataccacgc tggtggtgat tgctcaccgc ttatcgacca
    20341 ttgttgatgc cgacaccatt ctggtgcttc atcgtgggca agccgtggag cagggcactc
    20401 accagcaact gctggcggcc cagggacgct actggcagat gtatcaactg caacttgcgg
    20461 gcgaagagct ggcagccagc gtgcgtgaag aagaatcatt gagcgcctga atagcgcaat
    20521 atttcatcgt tggtgcaaaa atgtaacgca ctgtgcactg tcatagtgcg ttttcatttt
    20581 caatcttctt aacttcctgc tctctttctc gtttttcatt tctggcacac cgcttgcaat
    20641 accttcttcg tgtagcagaa ccattaccga attctgaccg gaggggatct atgaagctgg
    20701 tgaccgtgat aatcaaacca ttcaagctgg aagacgttcg tgaagcgtta tcttccattg
    20761 gtattcaggg cctgaccgtc accgaagtga aaggtttcgg gcgtcagaaa gggcatgccg
    20821 agctgtaccg gggggcggaa tacagcgtca acttcctgcc aaaagtaaaa attgatgtgg
    20881 cgattgctga tgaccaactc gatgaagtga tcgatatcgt cagtaaggcg gcttacaccg
    20941 gaaaaattgg cgacggcaaa atcttcgtcg ctgaattgca acgcgtcatt cgtattcgta
    21001 ccggcgaagc cgacgaagcg gcgctgtaat ctctggcaca cagcaacagg aacgaaaaat
    21061 gaagatagcg acgataaaaa ctgggcttgc ttcactggcg atgctgccgg gactggtaat
    21121 ggctgcacct gcggtggccg ataaagccga caatgcgttt atgatgattt gtactgcgct
    21181 ggtgctgttt atgactattc cggggattgc cctgttttac ggtgggttga ttcgcggcaa
    21241 aaacgtgctg tcgatgctga cgcaggtgac ggtgacgttt gcactggtct gtattctctg
    21301 ggtggtttac ggttactcgc tggcgtttgg tgagggcaac aacttcttcg gcaacattaa
    21361 ctggttgatg ctaaaaaaca tcgaactgac ggcggtgatg ggcagcattt atcagtatat
    21421 ccacgtggcg tttcagggct cgtttgcctg cattaccgtc ggcttgatcg ttggggcgct
    21481 ggcggaacga atccgcttct cagctgtgtt gattttcgtg gtggtatggc tgacgctctc
    21541 ttacattccg attgcgcata tggtgtgggg cggtggtttg ctggcttctc acggtgcgct
    21601 ggatttcgcg ggtggcacgg tggtgcatat taacgccgca atcgccggtc tggtgggcgc
    21661 gtatctgata ggcaaacgcg tgggcttcgg caaagaggcg tttaaaccgc acaacctgcc
    21721 aatggtcttc accggtactg ccattctcta tattggttgg tttggcttta acgccgggtc
    21781 agcgggcacg gcgaatgaaa tcgcggcact ggcatttgtg aatactgtgg tcgcaacggc
    21841 ggcggcaatt cttggctgga tcttcggtga atgggcgctg cgtggtaagc cttctctgct
    21901 gggagcgtgt tctggcgcga ttgccggtct ggtcggcgtg acgccagcgt gtggctacat
    21961 tggggttggc ggcgcgttga ttatcggcgt ggtagctggt ctggcgggct tgtggggcgt
    22021 tactatgctc aaacgcttgc tgcgggtgga tgatccctgc gatgtcttcg gtgtgcacgg
    22081 cgtttgtggc attgtcggct gtatcatgac tgggattttt gccgccagtt cgctgggtgg
    22141 cgtgggcttc gctgaaggtg tgacgatggg ccatcagttg ctggtacagc tggaaagcat
    22201 cgccattacg atcgtctggt ccggtgtggt ggcatttatt ggctacaaac tggcggatct
    22261 gacggttggt ctgcgtgtac cggaagagca ggagcgagaa gggctggatg tcaacagtca
    22321 cggcgagaat gcctataacg cgtaacaagc actgcaaaaa atagccggac ggttttacct
    22381 ccggctattt ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag
    22441 cacgccgtcc tgggtataaa actcaccgcg cacaaagccg cgtgcgctgg acgccgaggt
    22501 gctctccacg ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga
    22561 atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag
    22621 agctaccggc aggaagttaa gatcagaagc gtaaccgagc agatactgat gaacgcgcag
    22681 gtcatccggc acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg
    22741 acctttcagt gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa
    22801 tttatcttta agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttccga
    22861 aggcaggcca tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc
    22921 tggtgcctgg aaagaggccg tcatataaaa aatcggtttg ccgttttgaa tagcagcaac
    22981 ccggcgggcg ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt
    23041 cttactatcg ccagggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttcagg
    23101 gacggtctct tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg
    23161 taaacctaaa tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa
    23221 caatgtcagt aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc
    23281 agcaagccaa agtgagttga gtataacgca aatttgctac tggtccgatg ggtgcaatgg
    23341 tctgaattac gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt
    23401 actattttgt taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa
    23461 ggagaaatga atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc
    23521 ggcttgcgca gataaaagcg cggatattca gacgccagcc ccggctgcaa atacgtctat
    23581 ttcagcaaca caacaacctg ctatccagca accgaatgtc tccggtaccg tctggatccg
    23641 tcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg acactttctg acgcgtcgtt
    23701 agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag gtaaacagtc
    23761 accattcagc tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct
    23821 gttgagtgcg gcgattaccg tgaatgacaa actggtattt attaccgata ccgttcagcc
    23881 ggtgatcaac aagggcggaa ctaaagccga cctgacattg gtgccggtgc agcaaaccgc
    23941 cgtgccggtt caggccagcg gtggtgcaac gactaccgta ccttcgactt caccaactca
    24001 ggtgaatccg tcttcggcag ttccagctcc tacgcaatat taagcgacgt taaccctctc
    24061 cgcccggaga gggttagtag ttccagcgat aacgctgcaa gtcgatttgc ccgcttcccg
    24121 ataccatcac accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtta
    24181 gcgaaattgt gccgtggcga ttaaccaccc ggtgccaggg taaggtgctg ccttcaggga
    24241 gacgctttaa cacaccgccc acctggcgcg cggcgcgggg cgatcccgcc agtttcgcca
    24301 catcaccgta agtggtgaca tagccttcgg gaatagcggc gacgatttgc cagacgcgtt
    24361 ggggaaatga atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg
    24421 gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag catctggcac gcgatgggtt
    24481 gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg
    24541 ggggctctgt tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag
    24601 cagccaaggc agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg
    24661 cctcccaccg tttcgtcaaa aaaacccaac atggctaaac tttaaccaca actgacgtcg
    24721 caagaattgt ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg
    24781 ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc
    24841 tgcgccattg tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt
    24901 accggatggc aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt
    24961 tgtctttagc tggattgaat acccttcaaa agaggtccgc gacgctgcta ctcaaaagat
    25021 gatgtcggac ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat
    25081 gatctatggc ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg
    25141 tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg
    25201 taatgccttg ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtagccactc
    25261 ttcttgttta ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat
    25321 ttcgatgata tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat
    25381 cttcagaatg tcgacatcca gatcctgtaa ataacttaaa cttgaatatc ccgtaccaaa
    25441 atcatcaaga taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt
    25501 tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg
    25561 atagtgattg atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga
    25621 ttcaagattg atcgaaatat gctgctgtgg atgctgacgc agccagtcgc ccatatcttc
    25681 aaagacgctt cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag
    25741 cggaataaaa ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc
    25801 ctcagcaccg acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat
    25861 atcacgattt tcgatagcat cctgcagccg atgatgcggt gactggatac ggcgcaaaat
    25921 acgcagcaca aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa
    25981 gacttgccga tgccagcctt tctgcaacat tttcgttgaa gcccaggtga tgatcgaaat
    26041 attcatctcc gggaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc
    26101 gggtgttttt tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac
    26161 aacgttatgg gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac
    26221 atcaataaag gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat
    26281 gtaacggata atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga
    26341 aattttacct ggctcgggga aggtatcggg cggactttca tgctccagag acgaacactg
    26401 gggaacgttg ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg
    26461 acgcatttcc atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcttg
    26521 cagcgcatct ttcccttgtg tcgccacctt attggcccga atagcgacgc gggaggaata
    26581 ggtatccagc tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa
    26641 gccgacaggc agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg
    26701 tgttctcacc agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa
    26761 aaggctggca ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga
    26821 tcattgtcat ttgaccaggc gcagagcttt acgcctggta tgtaacccta acttaagatg
    26881 ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct ttacggacga
    26941 tcattttttg cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt
    27001 caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga
    27061 aatccagcac gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac
    27121 cattaagcag gataaataat acaaccagta gccagacaga gcctgacagc gtttcggact
    27181 gcaacatcac cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat
    27241 gtttacggat aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca
    27301 gagactcaat cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta
    27361 tctgcaactt tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag
    27421 atttatcaca attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg
    27481 gctggattac cgcccacgac aacgttgtcc gggacatctt ttgtgacaac tgcacctgag
    27541 gctaccacga cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc
    27601 cagacgttat taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca
    27661 gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca
    27721 ccgatgcgaa tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattatta
    27781 ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg gctcaatata agcctctgtc
    27841 acctgaccga atagatcagc gagaatttgc tggcgtaatg tgtgctcttc cgccagggaa
    27901 tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct
    27961 gccgagcgat acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc
    28021 cggttgctga aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttcccat
    28081 tacatctgtg tgataaagat cacatagggt atgtcttcat ggcgaaaaag tataaaattc
    28141 ttaataaaca gccggttata gctccgaaag cgaattattt attagcgaat aaatttccat
    28201 actgaggaag ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct
    28261 gcggctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata
    28321 acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttc
    28381 gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac aagataaact
    28441 cgcaggattc tctttcgtcg cattgacaaa acaacggaat agtcgattac ctgacttccg
    28501 ccatttctga agatcctgca tattaatacc ataactactg aacaacataa aggtgtcatc
    28561 cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt
    28621 aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac tggaggttga tcgccgaggt
    28681 tgggtcgtta acccagccat gattgctttc ttcaaggttt gcaaggcagt cacgatacag
    28741 ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat
    28801 aacgcgtccc cttcttagcg gttgaactaa cggacacctt tcgggatgga aaaaacttac
    28861 tgacctggac ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc
    28921 atcgcattat tacgaaaaat tacaattagg aaggaatcgg gacaggactc actttttcat
    28981 actagcacta acccgcagca ggtaaaagca gtctgctcga actgttcata acatttcagg
    29041 attatcgcca gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata
    29101 accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctctaa atgattatgg
    29161 attattaaat ctattagcgc tacttaatat aaattaacta acaatcagta ttttttatga
    29221 attttatccg tggttaatac tggttttcgt atgagatcct gagttggtgg ttcaattact
    29281 ccttaatgtt cgtaggttat gcataaaaaa ggccgcttgc gcggccttag tgattacacg
    29341 ttgtatcaat gatgatcgac agtatggctg tgctcgatat cttcattctt gcggctaaag
    29401 cggcggcgaa ccacaacaaa gaataccgga acgaagaaga ttgccagtac cgttgcggtc
    29461 accatcccgc ccattacacc ggtacctact gcgttctgcg cgccggaacc agcaccagta
    29521 ctgataacca gcggcataac gccgaggata aacgccagcg aggtcatcag gatcggacgt
    29581 aaacgcatcc gcactgcatc aagcgtcgct tcaatcagac ctttaccttc tttatccatc
    29641 aagtctttgg cgaattcgac gataagtatc gcgttcttcg ccgacaaccc aatggttgtg
    29701 agcaggccta cctggaagta aacgtcattg gtcaggccac ggaaggtggc agccagcaac
    29761 gcaccgataa cccccagcgg aacgaccagc ataacggaga acggaatcga ccagctctcg
    29821 tacagcgccg ccagacacag gaatacgaca atcaacgaaa tcgcgtacag tgaaggtgcc
    29881 tggttgccgg agaggcgttc ctgataggac atccccgtcc agtcatagcc aacaccggta
    29941 ggcagtttgc tcgccagttg ttccattagc tccattgctt caccggtact tttacccggt
    30001 gccgcctggc ctaagatttc catggatggc aggccattgt aacgttccag acgcggcgaa
    30061 ccgtactccc aacgagaaga ggagaatgcc gagaacggca ccatctgacc atcagcagca
    30121 cgaacatacc agtcgccgat atcatccggc agcatacggt atttcgcttc tgacatgacg
    30181 taaactttct tcacacgacc gcggtcgata aagtcgttca catagctacc gccccatgca
    30241 gcgcccagag tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg
    30301 tcgatatcaa tcttaaactg cggggtatct tccagaccgt ttggacgtac gctggtcagc
    30361 atatcagggt gcttcgctgc ttctgcaagc agctggttac gcgcctgagt cagtttttcg
    30421 tgaccaaggc cagcctggtc aatcagctca aagtcaaagc cggttgcagt acccagttcc
    30481 acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga gaaagcgcgt
    30541 gttgcacgca tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc ggcccagtcc
    30601 ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc gaagccgtta
    30661 acggcgaaca ccgactcaac gttgttcttt tctttggtca gatagtaatg cgttacctca
    30721 ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac catggtcata
    30781 aatacgccct ggtcctcatc tggcaagaag gagcttggca gacgcacgaa cagataggcc
    30841 atgccgacca cgatgatcag atacagcacc aggtaacgcc ccgtactgcg cagaataccg
    30901 cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa ccagccgaag
    30961 aagccttttt taccttcccc gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa
    31021 agagccggag tcaggatcaa cgctaccagt accgacagcg ccattgctga aacaatggta
    31081 atagagaact gacgatagat agcaccagta gaaccgccaa agaaggccat cggtacgaat
    31141 accgccgaca gtaccatcgc gataccgacc agagcgccct gaatctgccc catcgactta
    31201 cgggtagctt cttttggcgg caaaccttct tccgccataa cacgctccac gttttctacc
    31261 acaacgatag cgtcatccac caacaagccg atagcgagca ccatcccgaa cattgttagc
    31321 gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa taccaccggt
    31381 acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca ggaacagata cataaccagg
    31441 aacacgagga tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga gattttcacg
    31501 aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacccg acgggaagaa cggttccatc
    31561 ttcgccagtt cagcacggat tgccgcagcg gtatccagcg cgtttgcacc ggtcgccagc
    31621 ttgatcccca gaccggaagc cggttggccg ttaaactctg cgatgatgtc gtagttctca
    31681 ccacccagct caatcttcgc cacatcacgc agcagtacgc gggaaccatc ttgattcact
    31741 ttcagcagga ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc aataatagag
    31801 gcgttaagct gttggccttt caccggcggc gtaccaccga gctgacccgc cgcaacctgg
    31861 gcgttctgcg ctttgatggc ggtaatgaca tcaaccggcg ttagctggaa tttgttcagc
    31921 tcattcgggt tcatccagat acgcatcgcg tactgtgaac cgaacaactg aacatcaccc
    31981 acgcccgacg tacggctgat ggcatctttc atattcgccg ctacgtagtc agagatatcc
    32041 tcctgcgtca tggtgccatc ggtgttgata acgccgacaa ccatcaggaa gctgctggat
    32101 gatttctcaa cgctcacccc ttgctgctga acttcttgcg gcagcaacgg catcgccagc
    32161 tgcagtttgt tctgcacctg aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc
    32221 agggtgatct gcacggtacc cgtggagtca ctgttagagg acatgtacat caggttatcg
    32281 ataccgttca tattctgttc gataacctgt gtcaccgtgt cctgcactgt tttcgcatca
    32341 gcgccggggt aggaggcgga gatcgttact gccggcggtg caatcgtagg atattgcgcc
    32401 accggcagtt tgaggatcgc cagcccccct gccaacatga tgataatggc gatcacccac
    32461 gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt aacggctcct gtttaagtta
    32521 agacttggac tgttcaggct gagcaccgct tgcggcttgc tggttattat cagcggtaac
    32581 ttcttgtgct tttacctgga caccaggacg cactttctgc agcccactta ttactacgcg
    32641 atcgcctgct ttcagacctt ctgtcaccag ccacttatcg ccaatagcct ggcttgcaac
    32701 gatcggacgg gtttccactt tgtcatccgc gccaactacc agtacggtgg catcaccacg
    32761 cggcgtacgg gttacgccct gttgcgggac taaaatagcg tttggattaa gcccttcttc
    32821 cagacgtgcg cgcacgaaca tacccggcag cagagtgtga tccgggttcg ggaagatagc
    32881 gcgtagggtg atagacccag tggtctgatc aacggtaacg tcagagaatt ccagcgtacc
    32941 gtcctgcggg aacttaatgc cgtcactggt gatcagcgac actttggctt tgccgttctc
    33001 ttgtttcagc gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt tgctggactg
    33061 ggtcacatca acgtagatcg gatcaagttg ctgcacggtt gccagcgcag tcgcctgacc
    33121 gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc taatcggaga
    33181 ggtgactttg gtgtaagcca gattgatccg cgcagtttca acggcagctt tcgccgcagt
    33241 taccgcagca ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt gcttactgat
    33301 gtactgagta ccgagcagtt tctgataacg attcaccgtc aattgcgcga tattggctgc
    33361 agcctgggct ttcgccagat cacctttcgc actgtcgtat gtcgcctgat aggtcgcagg
    33421 atcaatctga tagagagaga cacctgcttc gatgtcgcta ccttctttga aattacgctt
    33481 caggataatc ccgctaactt gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc
    33541 cggaagctcg gttgtgatct gcagaggttc agttttgact gttactacgc caacggcggg
    33601 catctgctgg ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc
    33661 tgagagcatc agaacgaccg ccagaggcgt aaaccctctg tttttgttca tatgtaaacc
    33721 tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt
    33781 atattatcgt cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa
    33841 attcacgaac atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc
    33901 ctcgatgtgg ctctacgtct tttctcacag cagggggtat catccacctc gctgggcgag
    33961 attgcaaaag cagctggcgt tacgcgcggt gcaatctact ggcattttaa agacaagtcg
    34021 gatttgttca gtgagatctg ggaactgtca gaatccaata ttggtgaact agagcttgag
    34081 tatcaggcaa aattccctgg cgatccactc tcagtattaa gagagatatt aattcatgtt
    34141 cttgaatcca cggtgacaga agaacgacgt cgattattga tggagattat attccacaaa
    34201 tgcgaatttg tcggagaaat ggctgttgtg caacaggcac aacgtaatct ctgtctggaa
    34261 agttatgacc gtatagaaca aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg
    34321 gatttaatga cgcgtcgggc agcaattatt atgcgcggct atatttccgg cctgatggaa
    34381 aactggctct ttgccccgca atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc
    34441 atcttactgg agatgtatct cctgtgcccc acgcttcgta atcctgccac taacgaataa
    34501 ccctgaatct gactccagga tttttcctgg acattttcgt cgttgctatt ctggttcact
    34561 gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt cttcaggttc agaaaccttc
    34621 attcatcatg actatgttcc agtattacaa acgatcacgg cattttgttt tttcagcatt
    34681 tattgctttt gtttttgtct tgttatgcca gaacacggcg tttgcgcggg cgtcatcgaa
    34741 tggtgatctg ccgacaaaag cggacctgca ggcgcaactt gactcactaa ataaacaaaa
    34801 agatctttct gctcaggaca aactggtgca gcaggatctg acagatacat tagccaccct
    34861 cgataaaatc gatcgcataa aagaagagac agttcagcta cggcaaaaag tcgctgaagc
    34921 gccggaaaaa atgcgccagg cgaccgcggc gttaacagca cttagcgatg tcgataacga
    34981 cgaagaaacg cgcaaaattc tgagtacgct ttcgttgcgc cagctggaaa ctcgcgttgc
    35041 ccaggcgctg gacgatttgc aaaacgcaca aaacgatctg gcgtcttata acagccagct
    35101 ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg cttcgcagca
    35161 gctgcaacaa attcgcagtc gtctggatgg gactgatgtc ggcgagactg ccttacgtcc
    35221 cagccagaaa gtgttaatgc aggctcagca ggcgttgctg aatgcggaga ttgaccagca
    35281 gcgtaaaagc ctggaaggga acaccgtctt gcaggatacc ttgcaaaagc aacgtgatta
    35341 cgtgacggcg aacagcgccc gtctggagca ccagttgcag ctgttgcaag aagcggtaaa
    35401 cagcaagcgc ctgactttaa ctgaaaaaac ggcgcaggaa gccgtctccc cggatgaagc
    35461 cgcgcgtatt caggctaatc cgctggtgaa acaggaactg gaaattaacc agcagttaag
    35521 tcagcgtctg attaccgcga ctgaaaacgg taatcagttg atgcagcaaa acattaaagt
    35581 caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat attaaagagc agattgccgt
    35641 cctgaagggc agcctgctgt tgtctcgtat cctttaccag caacaacaaa cgctgccctc
    35701 ggcggatgaa ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg aacagtttga
    35761 agttaaccag cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca aactggaaga
    35821 aggtcacacc aacgaagtca acagcgaagt tcacgatgcg ttattgcaag tggttgatat
    35881 gcgtcgcgaa ttgctggatc aactcaacaa acagttgggt aaccagctga tgatggccat
    35941 taacctgcaa atcaaccagc agcagttaat gagcgtatcg aaaaacctga aatccatcct
    36001 gactcagcaa atcttttggg tgaacagtaa ccgtccaatg gactgggact ggatcaaagc
    36061 gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa atcacggtga actgggaaaa
    36121 agcctggcct gccgttttta tcgctttcct cgctggtttg ccgctgctgt tgattgccgg
    36181 gctgatccac tggcgtctgg gctggctgaa agcgtatcaa cagaaactgg cttccgctgt
    36241 gggttccctg cgtaacgata gccagctcaa cacaccaaaa gcgatcctta tcgacctgat
    36301 ccgcgcgctg ccggtgtgcc tgattattct cgcggttggc ctgattctgt tgaccatgca
    36361 gctcaacatc agcgaactgc tatggtcgtt cagtaaaaaa ctggcgattt tctggctggt
    36421 gtttggcctg tgctggaagg tactggagaa aaatggcgtt gccgtgcgtc acttcggcat
    36481 gccggagcag cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc tcgcgttgct
    36541 gcctatccat ttctggtctg tggtggcaga actttccccg ctgcatctga tggatgatgt
    36601 gctggggcag gcgatgattt tcttcaacct gctgctgatt gccttcctgg tatggccaat
    36661 gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg cgactggtca ccattaccgt
    36721 gctgtcgatt atcccgattg cattgatggt gctgactgcc acgggttatt tctacaccac
    36781 gctgcgcctc tccgggcgtt ggattgaaac cgtttatctg gtgatcatct ggaacttgct
    36841 ctaccagacg gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc
    36901 gctggcgcgt cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac cgccggaaga
    36961 acccaccatt gcactggagc aagttaacca gcagacgctg cgtattacca tgttgctgat
    37021 gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc gatttgatca ccgtgttcag
    37081 ctatctcgac agcatcacgc tctggcatta caacggcact gaagctggcg ctgcggtggt
    37141 gaaaaacgtc accatgggca gtctgttgtt tgcgattatc gcctcaatgg tggcctgggc
    37201 gttgattcgc aacctgcctg gtttactgga agtgctggtg ctctcgcgac tgaatatgcg
    37261 tcagggcgcg tcgtatgcca ttactaccat ccttaactac atcattattg ccgttggtgc
    37321 gatgacggtg ttcggatcgc tgggcgtctc ttgggataaa ctccagtggc tggccgcagc
    37381 attatccgta ggtcttggtt ttggtttaca agaaattttc ggtaacttcg tctccggttt
    37441 gatcattctg ttcgaacgtc cggtgcgtat tggcgatacg gtaaccattg gtagcttctc
    37501 ggggacggta agtaagatcc gtattcgtgc gacaacgatt accgatttcg atcgcaaaga
    37561 agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg atcaactggt cgttgactga
    37621 cactactacg cgtctggtga tccgtctcgg cgtggcctat ggctccgatc tggaaaaagt
    37681 gcgtaaagtg ttactgaagg cggcgactga gcacccaagg gtgatgcacg aaccaatgcc
    37741 ggaagtcttc tttacggcgt ttggtgccag cacgttggat catgagctgc gtctgtatgt
    37801 gcgtgaactg cgtgaccgta gtcgtactgt cgatgagctg aaccgtacta tcgatcagct
    37861 gtgccgtgaa aacgacatca acattgcctt taaccagctt gaagtgcatc tgcacaacga
    37921 gaagggcgat gaggtgacgg aagtcaaacg cgactacaaa ggcgatgacc cgacgccagc
    37981 ggtagggtaa taaccaaagg gggcgacatt tagttgcccc gagattactg acaaagtgcg
    38041 cgttgttcat gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc gcaagaattc
    38101 aatgtattgc aggagcggtg taggcctgat aagcgtagcg catcaggcag ttttgcgttt
    38161 gcccacaatc ttaggggaca tttagcggcc ccatttattt ctcacttttc cgcctcatca
    38221 tcgcgcgtta atttcttttc ataatcgcgt tttacaatat ccagcgcgcg cagcacggtg
    38281 cgggctggga tctgattttc ttccagcagc acaatcaaat cgacagccag tttgacatcg
    38341 tcaggggcat tttccagtga catactctct ccattgctag cgggttaagc gcgctagcct
    38401 gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat aggcttccac
    38461 ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccaggc gggctctgcg
    38521 ttcggcaacc atctcacgta gccgccgctc aaaatcctga tgctgaatac gtttacgctg
    38581 ccagcgggca attttcggtg gcgcactgtc ccactcgcgc aatgaccagg cggaggcttc
    38641 acgcgcgatg gcttccagtt gtgccgccag atgttccgcc agccaggcca cttgcggcag
    38701 ctgttgctgc tcaactgcat gacgaagcgc agccagatta tcgcccgcct catcgagaca
    38761 agcctgcagt gttgtcgcac gggtttgaaa aaggtgtctg tcaaaacggg cacttagcgt
    38821 ggcaaattgc gctaccgggg cacaacgctg acgcagcgta gcgagctgtc cttccagttt
    38881 ttccagcagc agggcggttt tcacgataag acctcaatga aaatgataat tgttatgcta
    38941 aagtagccac tctgtaagct gacaacactc aatatgcaac gaatcatttt aatcatcatt
    39001 ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt accgacgacg
    39061 ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt tcacgcctgg
    39121 ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca tcatgcgatg
    39181 ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc catttctctg
    39241 tggttcgtcc agatgccatg ggtgcgcatc atgttgctgg taattctcgc ctgtttgctt
    39301 ttctatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg aagcacaaca
    39361 atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag cacaggcgca
    39421 ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc gtgagtacca
    39481 ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag tatctcaaaa
    39541 atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgc gatgtcacca
    39601 gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt gagcgttaca
    39661 aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg tttggcgctc
    39721 cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc aaactgccgc
    39781 gtgaaaccat cagtgaaact tacgacctgg aatacggcac cgatcagctg gagatccacg
    39841 ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg gcaaccggcg
    39901 gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg gctgacgctg
    39961 cgttcattat caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa cagggcatta
    40021 ccagctacag ccttgtcccg ttcccgggcc attaattatc gccagtcttg tgctgcccac
    40081 gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct gtgttagcat
    40141 taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt atcaggtctt
    40201 agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac atgtgctgac
    40261 cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt tttccggtac
    40321 ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa actgcgaaac
    40381 cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg agcaggggcg
    40441 ctttgtcgat ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag atacccgcga
    40501 cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt atctgatcga
    40561 cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc ttgaagagcc
    40621 gccggagcac gttaagtttc tgctggcgac gaccgatcca cagaaattgc cggtgacgat
    40681 tttgtcgcgc tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa ttcgccatca
    40741 acttgagcac atcctcaacg aagaacatat cgctcacgag ccgcgggcgt tgcaattgct
    40801 ggcgcgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc
    40861 cagcggtgac ggccaggttt caacccaggc agtcagtgcg atgctgggta cgcttgacga
    40921 cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc gcgtaatggc
    40981 gctgattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct
    41041 cggcctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat
    41101 ggccgccatt gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca
    41161 gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cggaccgccg
    41221 catgggcgtt gagatgacgc tgctgcgcgc gctggcgttc catccacgta tgcctctgcc
    41281 tgagccagaa gtgccacgcc agtcctttgc gcccgtcgcg ccaacggcag taatgacgcc
    41341 aacccaggtg ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg taccgctccc
    41401 ggaaaccacc agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac
    41461 caaagcaaaa aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc
    41521 gctggaaaga ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct
    41581 ggaaaaagcg ccagccaaaa aagaagcgta tcgctggaag gctaccactc cagtgatgca
    41641 gcaaaaagaa gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac
    41701 gccggaactg gcggcgaagc tggcggcaga agccattgag cgcgacgcgt gggcggcaca
    41761 ggtgagtcaa ctttcgctac caaaactggt cgaacaggtg gccttaaatg cctggaaaga
    41821 ggagagcgac aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg
    41881 cggtgcacag caaaaactgg ctgaagcgtt gagcacgtta aaaggttcaa cggttgaact
    41941 gactatcgtt gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat
    42001 atacgaagaa aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac
    42061 cctgcgtcgg ttcttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta
    42121 agcacagctt acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag
    42181 gcggtctggg taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc
    42241 aggaagagat cgcgcagctg gaagtcaccg gcgaatctgg cgcaggtctg gtaaaagtga
    42301 ccatcaacgg tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg
    42361 acaaagagat gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg
    42421 aagaaacgca gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct
    42481 ttaagatgcc gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg
    42541 ctgtctgccg ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg
    42601 cgatcgtagc ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg
    42661 ccactgcgcc gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc
    42721 gcgtcgtcag gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat
    42781 tgagcagacg gggcagtttt caggccgtta ttttgtgttg atggggcacc tgtcaccgct
    42841 ggacggcatc ggtccggatg atatcggcct tgatcgtctg gaacagcgtc tggcagagga
    42901 aaaaatcact gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa
    42961 ctacattgcc gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt
    43021 accggttggc ggcgaactgg aaatggtcga tggcaccacg ctgtcacact cccttgccgg
    43081 gcgtcataag attcgttttt aagcaaacga gagcaggatc acctgctctc gcttgaaatt
    43141 attctccctt gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga
    43201 ggtagaccta catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc
    43261 tgcacctgat gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct
    43321 ctaacgcctc cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt
    43381 acgaaggtga tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga
    43441 ccatctccga taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta
    43501 tcgctaaatc cggtaccaaa tcattcctcg aatccctggg ttctgaccag gcgaaagaca
    43561 gccagctgat cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag
    43621 tgaccgtgcg tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg
    43681 ctggcgaagg tgaatatacc gttgccgaca tcaccaaaga agatcgtggt actgaaatca
    43741 ccctgcatct gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca
    43801 tcagcaaata ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag
    43861 acggcgaaac cgttatctcc tgggagaaaa tcaacaaagc gcaggcgctg tggactcgta
    43921 acaagtcgga aatcaccgat gaagagtaca aagagttcta caaacacatc gcccacgact
    43981 ttaatgatcc gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc
    44041 tgctgtacat cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc
    44101 tgaaactgta cgtccagcgt gtgttcatca tggacgacgc agaacagttc atgccgaact
    44161 atctgcgctt cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg
    44221 aaatcctcca ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc
    44281 tgcaaatgct ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac
    44341 agtttggcct ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg
    44401 ccaaactgct gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg
    44461 aagactacgt ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca
    44521 gctatgcggc agcgaagagc agcccacacc tggaactgct gcgtaagaaa ggcatcgaag
    44581 ttctgctgct ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg
    44641 gtaaaccgtt ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag
    44701 ttgatgagag cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgtgtgaaag
    44761 ccctgctcgg cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag
    44821 cgatcgtttc gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg
    44881 cgggccagaa agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg
    44941 tgaaacgtgc ggcagatact gaagatgaag cgaagttcag cgagtgggta gaactgctgc
    45001 tggatcaggc gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc
    45061 gtatgaacca gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat
    45121 ttttcttttc atcatctgca ctttccgcaa attatctcgc cattaaccgt ttcagcctca
    45181 ggtgcctttc ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaatt
    45241 cgacacattt taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg
    45301 ggaaagggac tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg
    45361 gcgatatgct gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca
    45421 ttatggatgc tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca
    45481 ttgctcagga agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc
    45541 aggcagacgc gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac
    45601 cggacgaact gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg
    45661 tttatcacgt taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag
    45721 aactgactac ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc
    45781 atcagatgac tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca
    45841 aatacgcgaa agttgacggc accaagccgg ttgctgaagt tcgcgctgat ctggaaaaaa
    45901 tcctcggcta attcgaaagc gcgcacggac agtcccctcg ccccctcggg gagagggtta
    45961 gggtgagggg aacaggcccg cacaagcaaa cttatcagca atctcaggcc ggatattcat
    46021 tcggcctttt acaaaaacct caatctgcgc tattcctttt tctgatttga cctctcacag
    46081 caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa tcgataagag
    46141 gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac gcccgatgcc
    46201 cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag acgcgtggtt
    46261 gatacctcac ggttgttatg gtggccgttg ctgcgcggcg tgattttgcc gctgcgctcg
    46321 ccgcgtgtgg cgaagctgta cgcctcggtc tggatggaag acggctcgcc gctgatggtt
    46381 tacagccgcc agcaacagca ggcgttggca caacgtttac cggatacgcc cgtagcgctg
    46441 ggcatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct ggcagagcat
    46501 gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac ggtcggtgcg
    46561 gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg gatatcgttt
    46621 attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag cgtacgcgct
    46681 tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg cattccccag
    46741 cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac tcgcgaactg
    46801 gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc gcgctttggc
    46861 cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg agaaaaaggc
    46921 gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga gacgctggaa
    46981 gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa atatgaatat
    47041 attccagcgc ttaatgccac gccggaacat atcgaaatga tggctaatct tgttgccgcg
    47101 tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc gtcggcggtt
    47161 ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag tttgaactca
    47221 cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc cagcagcgga
    47281 tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc attattaaac
    47341 agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc ttcatacatc
    47401 tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg agtcacggaa
    47461 tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc gcaatcgatc
    47521 tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc ggaatcaccg
    47581 gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg gaaataacaa
    47641 caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga aagggggtaa
    47701 tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat gcgatcgtgg
    47761 gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt cgccgggcta
    47821 tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg aaccatgtaa
    47881 gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag cgtgtaatac
    47941 tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg ctgaagagta
    48001 ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt gttttccggc
    48061 ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca tgaaaatgcg
    48121 atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag attgtgttaa
    48181 cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg taaacgtaaa
    48241 tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt ccagccagaa
    48301 aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga tattgaagcg
    48361 aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc actggtgatt
    48421 gaggatgatg tagccgaagc gctttatcag gaactaaaac agaaaaacct gattacccat
    48481 cagtttgcgg gtggcaccat tggtaacacc atgcataact actcggtgct cgcggacgac
    48541 cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagcta tgcctatcgt
    48601 tacctgtgta acacctccag ccgtaccgat cttaactatc tacaaggcgt ggatggcccg
    48661 attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc tatcagccct
    48721 ggccacatga accagctgcg ggctgaaagt attccggaag atgtgattgc cggagcctcg
    48781 gctctggttc tcacctctta tctggtgcgt tgcaagccgg gtgaacccat gccggaagca
    48841 accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct gacgctggga
    48901 actaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa agaccacgtc
    48961 tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag cgatccgttg
    49021 ttggcatctg acaaggcgct ggactgggta gatctggtgc tgtgcaccgc cgggccaatc
    49081 ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca gcatccgctg
    49141 ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat gcgccacaag
    49201 gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg cgggccggaa
    49261 aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct gcatgacatt
    49321 accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa attcacctgg
    49381 ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag ctatcaggta
    49441 ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga cagcctggaa
    49501 gagtcttact gggatcgtta agttatcgac cagaccggac gacgatggca tccgtaggcc
    49561 ggataagatg cgctagcatc gcatccggca ataacgtctt gttttacccc gtcaccacct
    49621 cacctgccgg tggcgtttcc agcaattcca gcatagtacg ggcaatttca cgttcaccca
    49681 tcactacctg attcgcgcca cgttcggtga tatacgtcac ttcatcgtca taatgggcgc
    49741 gggcgataat ctcaatatcc gggtttttcg cgcgggcaga cgccacaatc tcacccgctt
    49801 cataaccgtt ggggatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    49861 taatttcttc gttcgctgca ttgcccaata cagcgcggac cccacgctct cgcagctcat
    49921 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    49981 cccccagcag gctgcctaca cgaccgtaac ccaccaatag cgcatggttg caaatatcca
    50041 ctgggatctg tttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    50101 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    50161 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    50221 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    50281 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    50341 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    50401 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    50461 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    50521 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    50581 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    50641 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    50701 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    50761 ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    50821 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    50881 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    50941 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    51001 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
    51061 aaccgacgcc gaacatcagc aaaataacgc ccagttcagc cagttccggc gcaagcttgg
    51121 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    51181 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    51241 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    51301 gttattgtcc atttttggcc ggggaaacca aaattacagg taatagt
//
LOCUS       DAPPNJ010000004        48510 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000004 DAPPNJ010000000
VERSION     DAPPNJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.004"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            220..1437
                     /gene="alaA"
                     /locus_tag="RPM73_000146"
     CDS             220..1437
                     /gene="alaA"
                     /locus_tag="RPM73_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDX6518544.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1521..2120
                     /gene="yfbR"
                     /locus_tag="RPM73_000147"
     CDS             1521..2120
                     /gene="yfbR"
                     /locus_tag="RPM73_000147"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDX6518545.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2179..4011)
                     /locus_tag="RPM73_000148"
     CDS             complement(2179..4011)
                     /locus_tag="RPM73_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDX6518546.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4098..4748)
                     /gene="hxpA"
                     /locus_tag="RPM73_000149"
     CDS             complement(4098..4748)
                     /gene="hxpA"
                     /locus_tag="RPM73_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDX6518547.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4759..5253)
                     /locus_tag="RPM73_000150"
     CDS             complement(4759..5253)
                     /locus_tag="RPM73_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDX6518548.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5336..5791)
                     /locus_tag="RPM73_000151"
     CDS             complement(5336..5791)
                     /locus_tag="RPM73_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDX6518549.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6129..7331
                     /gene="ackA"
                     /locus_tag="RPM73_000152"
     CDS             6129..7331
                     /gene="ackA"
                     /locus_tag="RPM73_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDX6518550.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7406..9550
                     /gene="pta"
                     /locus_tag="RPM73_000153"
     CDS             7406..9550
                     /gene="pta"
                     /locus_tag="RPM73_000153"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDX6518551.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9740..11260
                     /gene="yfcC"
                     /locus_tag="RPM73_000154"
     CDS             9740..11260
                     /gene="yfcC"
                     /locus_tag="RPM73_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDX6518552.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVYPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11293..11835)
                     /gene="yfcD"
                     /locus_tag="RPM73_000155"
     CDS             complement(11293..11835)
                     /gene="yfcD"
                     /locus_tag="RPM73_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDX6518553.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11893..12444)
                     /gene="yfcE"
                     /locus_tag="RPM73_000156"
     CDS             complement(11893..12444)
                     /gene="yfcE"
                     /locus_tag="RPM73_000156"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDX6518554.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12500..13144)
                     /gene="yfcF"
                     /locus_tag="RPM73_000157"
     CDS             complement(12500..13144)
                     /gene="yfcF"
                     /locus_tag="RPM73_000157"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDX6518555.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13280..13927
                     /gene="yfcG"
                     /locus_tag="RPM73_000158"
     CDS             13280..13927
                     /gene="yfcG"
                     /locus_tag="RPM73_000158"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDX6518556.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13984..14346
                     /gene="folX"
                     /locus_tag="RPM73_000159"
     CDS             13984..14346
                     /gene="folX"
                     /locus_tag="RPM73_000159"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDX6518557.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14367..15260
                     /locus_tag="RPM73_000160"
     CDS             14367..15260
                     /locus_tag="RPM73_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDX6518558.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15308..16210)
                     /gene="rpnB"
                     /locus_tag="RPM73_000161"
     CDS             complement(15308..16210)
                     /gene="rpnB"
                     /locus_tag="RPM73_000161"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDX6518559.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16407..17180)
                     /gene="hisP"
                     /locus_tag="RPM73_000162"
     CDS             complement(16407..17180)
                     /gene="hisP"
                     /locus_tag="RPM73_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDX6518560.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17188..17904)
                     /locus_tag="RPM73_000163"
     CDS             complement(17188..17904)
                     /locus_tag="RPM73_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6518561.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17901..18587)
                     /gene="hisQ"
                     /locus_tag="RPM73_000164"
     CDS             complement(17901..18587)
                     /gene="hisQ"
                     /locus_tag="RPM73_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDX6518562.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYYTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18677..19459)
                     /gene="hisJ"
                     /locus_tag="RPM73_000165"
     CDS             complement(18677..19459)
                     /gene="hisJ"
                     /locus_tag="RPM73_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDX6518563.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19680..20462)
                     /gene="argT"
                     /locus_tag="RPM73_000166"
     CDS             complement(19680..20462)
                     /gene="argT"
                     /locus_tag="RPM73_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDX6518564.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20728..21297)
                     /gene="ubiX"
                     /locus_tag="RPM73_000167"
     CDS             complement(20728..21297)
                     /gene="ubiX"
                     /locus_tag="RPM73_000167"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDX6518565.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21392..22909)
                     /gene="purF"
                     /locus_tag="RPM73_000168"
     CDS             complement(21392..22909)
                     /gene="purF"
                     /locus_tag="RPM73_000168"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDX6518566.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22946..23434)
                     /gene="cvpA"
                     /locus_tag="RPM73_000169"
     CDS             complement(22946..23434)
                     /gene="cvpA"
                     /locus_tag="RPM73_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDX6518567.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23693..24355)
                     /gene="dedD"
                     /locus_tag="RPM73_000170"
     CDS             complement(23693..24355)
                     /gene="dedD"
                     /locus_tag="RPM73_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDX6518568.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24345..25613)
                     /gene="folC"
                     /locus_tag="RPM73_000171"
     CDS             complement(24345..25613)
                     /gene="folC"
                     /locus_tag="RPM73_000171"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDX6518569.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25683..26597)
                     /gene="accD"
                     /locus_tag="RPM73_000172"
     CDS             complement(25683..26597)
                     /gene="accD"
                     /locus_tag="RPM73_000172"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDX6518570.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26753..27412)
                     /locus_tag="RPM73_000173"
     CDS             complement(26753..27412)
                     /locus_tag="RPM73_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6518571.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27495..28307)
                     /gene="truA"
                     /locus_tag="RPM73_000174"
     CDS             complement(27495..28307)
                     /gene="truA"
                     /locus_tag="RPM73_000174"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDX6518572.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28307..29320)
                     /locus_tag="RPM73_000175"
     CDS             complement(28307..29320)
                     /locus_tag="RPM73_000175"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDX6518573.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29386..30522)
                     /gene="pdxB"
                     /locus_tag="RPM73_000176"
     CDS             complement(29386..30522)
                     /gene="pdxB"
                     /locus_tag="RPM73_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDX6518574.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVNEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAQLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30621..31616
                     /gene="flk"
                     /locus_tag="RPM73_000177"
     CDS             30621..31616
                     /gene="flk"
                     /locus_tag="RPM73_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDX6518575.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMIDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31613..32791)
                     /locus_tag="RPM73_000178"
     CDS             complement(31613..32791)
                     /locus_tag="RPM73_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6518576.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33056..34276)
                     /gene="fabB"
                     /locus_tag="RPM73_000179"
     CDS             complement(33056..34276)
                     /gene="fabB"
                     /locus_tag="RPM73_000179"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDX6518577.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34435..36441
                     /gene="mnmC"
                     /locus_tag="RPM73_000180"
     CDS             34435..36441
                     /gene="mnmC"
                     /locus_tag="RPM73_000180"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDX6518578.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36562..36840)
                     /locus_tag="RPM73_000181"
     CDS             complement(36562..36840)
                     /locus_tag="RPM73_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDX6518579.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36874..37422)
                     /gene="epmC"
                     /locus_tag="RPM73_000182"
     CDS             complement(36874..37422)
                     /gene="epmC"
                     /locus_tag="RPM73_000182"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDX6518580.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37422..38231)
                     /locus_tag="RPM73_000183"
     CDS             complement(37422..38231)
                     /locus_tag="RPM73_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDX6518581.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38231..39055)
                     /gene="mepA"
                     /locus_tag="RPM73_000184"
     CDS             complement(38231..39055)
                     /gene="mepA"
                     /locus_tag="RPM73_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDX6518582.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGCFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39059..40144)
                     /gene="aroC"
                     /locus_tag="RPM73_000185"
     CDS             complement(39059..40144)
                     /gene="aroC"
                     /locus_tag="RPM73_000185"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDX6518583.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40179..41111)
                     /gene="prmB"
                     /locus_tag="RPM73_000186"
     CDS             complement(40179..41111)
                     /gene="prmB"
                     /locus_tag="RPM73_000186"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDX6518584.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41277..41828
                     /gene="smrB"
                     /locus_tag="RPM73_000187"
     CDS             41277..41828
                     /gene="smrB"
                     /locus_tag="RPM73_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDX6518585.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41950..42822)
                     /locus_tag="RPM73_000188"
     CDS             complement(41950..42822)
                     /locus_tag="RPM73_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDX6518586.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42809..43333)
                     /locus_tag="RPM73_000189"
     CDS             complement(42809..43333)
                     /locus_tag="RPM73_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6518587.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTSFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSAIVTATFEYF"
     gene            complement(43330..43800)
                     /locus_tag="RPM73_000190"
     CDS             complement(43330..43800)
                     /locus_tag="RPM73_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6518588.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43797..44345)
                     /locus_tag="RPM73_000191"
     CDS             complement(43797..44345)
                     /locus_tag="RPM73_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6518589.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44320..45072)
                     /locus_tag="RPM73_000192"
     CDS             complement(44320..45072)
                     /locus_tag="RPM73_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX6518590.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45092..47734)
                     /gene="yfcU"
                     /locus_tag="RPM73_000193"
     CDS             complement(45092..47734)
                     /gene="yfcU"
                     /locus_tag="RPM73_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDX6518591.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47816..48379)
                     /locus_tag="RPM73_000194"
     CDS             complement(47816..48379)
                     /locus_tag="RPM73_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6518592.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12028 a        13048 c        11986 g        11448 t
ORIGIN      
        1 acaattaaga atcagaacaa ttcactatat aacattgcat gtaaagcata tacacctcat
       61 tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg gcaaaatctt
      121 attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac
      181 ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc
      241 agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc
      301 ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccgttcggt
      361 tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg
      421 tattgcgatt ccaaaggtct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct
      481 cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt
      541 atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca
      601 gattacccac tatggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt
      661 tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct
      721 cgtacgcgtg ggatcgttat aatcaaccca aataacccaa ccggcgcggt atattccaaa
      781 gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat
      841 gaaatttatg acaaaattct ttacgacgac gctgagcatc actcaattgc gccgctggca
      901 cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc
      961 cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt
     1021 ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag
     1081 accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat
     1141 gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa
     1201 cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcacgac
     1261 gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg
     1321 gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat
     1381 atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct
     1441 taatttcact gccggagatt gcatccggca gcgttatccc gccacaatga cctgatgatg
     1501 tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga
     1561 aactcattaa ccgctggccg ctaatgcgca acgtgctgac ggaaaatgtg tccgaacaca
     1621 gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcga aaatttggcg
     1681 gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc
     1741 tcaccggcga tctccctact ccggtgaaat acttcaattc gcaaatcgct caggagtaca
     1801 aggctattga aaaaatcgct cagcaaaaac tggtcgatat ggttccggaa gagctgcggg
     1861 atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga
     1921 aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata
     1981 atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg
     2041 agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta
     2101 gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcaggcaat aagcgccgga
     2161 tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc
     2221 catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc
     2281 cggacctaaa accagtgtgt taacaggtga agaaaccggt gtcataaagg cggcggatgc
     2341 tgccatcgcc acgaccatcg cgaatggata aggcgacacc cccatcgttt tggcagcagc
     2401 cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaata gcccaataac
     2461 cgccgacaag acaaacaaac aacccagcat catatgtggc ccgtaaccac cgccaatgtc
     2521 catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg
     2581 catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc
     2641 atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg
     2701 aatttcatct gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc
     2761 ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc
     2821 gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc
     2881 gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat tgcgctttag
     2941 ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat
     3001 cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga
     3061 aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag
     3121 atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac
     3181 cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt
     3241 agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgaccc
     3301 ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg
     3361 aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa
     3421 acgcatcact aacatataca aaatacccag caccagcacg accaggccaa taggtgttac
     3481 gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt
     3541 cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat
     3601 catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa
     3661 gatagcgaca acgccggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa
     3721 caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg ttcccattac
     3781 ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac
     3841 aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc
     3901 aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa
     3961 gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc
     4021 ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta
     4081 aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta
     4141 atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga
     4201 gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca
     4261 tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac
     4321 gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg
     4381 ggaagcccag ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg
     4441 gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc cggaagcgcg
     4501 gtaatacctt cggtttccgt ggcctcgatg tgctccagac gcgtaaactc ggcggcaata
     4561 tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga
     4621 atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc
     4681 cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg
     4741 caccgcacgc gggcctcctc aggcattgat aatttgatta atttcgttgg cgctcaaatg
     4801 gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg
     4861 cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg gtatagcgcc cttccacatt
     4921 aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa
     4981 ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg aaacatgcaa
     5041 cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc
     5101 aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag
     5161 gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga
     5221 caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctatactacg
     5281 gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca
     5341 aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg
     5401 cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac
     5461 cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc
     5521 ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagcgcagtg gcaactgccg
     5581 gcccaagctg accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca
     5641 taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggggcaa
     5701 ggcgtttttc cagcggccac gtctttgagt aatgctgtcc ccggcgaaac aagctaaaaa
     5761 aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt
     5821 caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat
     5881 acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc
     5941 gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca tagactcaag
     6001 atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt
     6061 gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag
     6121 gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat
     6181 ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc
     6241 acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag
     6301 gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa
     6361 aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa
     6421 agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt
     6481 cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt
     6541 tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc
     6601 cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc
     6661 gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga
     6721 acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt
     6781 ctgctatccg caacggtaaa tgcgttgaca cctctatggg cctgaccccg ctggaaggtc
     6841 tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca
     6901 ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg
     6961 gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag
     7021 acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca
     7081 ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac tggtggtatc ggtgaaaatg
     7141 ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc
     7201 atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc
     7261 gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc
     7321 tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat
     7381 cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac
     7441 cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt
     7501 tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca
     7561 gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat
     7621 gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt
     7681 cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc
     7741 gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc
     7801 ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat
     7861 cgaactgacc cgcaacagct tcggcggtgc caaaaacacc aacatcaccg gcgttatcgt
     7921 taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc tgtccgagat
     7981 tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc
     8041 cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc
     8101 gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg
     8161 ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg
     8221 tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct
     8281 ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga
     8341 cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt
     8401 gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt
     8461 tgacgatcac gagcgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga
     8521 ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgcctgcgtt
     8581 ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa cgtatcgtac tgccggaagg
     8641 tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg
     8701 cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact
     8761 gggtgcaggg attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct
     8821 ggtcgaactg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agttggaaga
     8881 caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg
     8941 tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc
     9001 gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta
     9061 cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca
     9121 gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc
     9181 caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc
     9241 gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat
     9301 ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt
     9361 catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga
     9421 cctgatctcc atcgggccga tgctgcaggg tatgcgcaag ccggttaacg acctgtcccg
     9481 tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca
     9541 gcagcagtaa tctcgtcatc atccgcagct ttgcgctgcg gatatctgaa ccggaaataa
     9601 tcactatttc cggtttttta ttctcttaat ttgcattaat cctttctgat tatcttgctt
     9661 aactgcgctg catcaatgaa ttgcgccatc tcactttgca tacttaccac tttgttttgt
     9721 gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc
     9781 aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagcc ttgccacctg
     9841 ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac
     9901 aaaaacacgc aaagtcgtat atccacactc atttcgcatt ctgactaacg aagcaggcga
     9961 acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa
    10021 cttcccgttt gaaggattaa cctcaggatc gaaatacggg acagccgttg gcatcatcat
    10081 gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa
    10141 cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct
    10201 gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt
    10261 tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct
    10321 ggtgacctat attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt
    10381 ggtcgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt
    10441 ggtgtgggtt atcgccacta tgattggcct gatctttacc atggtgtacg cctcacgagt
    10501 gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca
    10561 ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac
    10621 cgccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat
    10681 tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct
    10741 taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat
    10801 cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggtcg gtaatggtga
    10861 agcgggtgat gccagcgtgt taaataccat cctcaacagc attgccaatg ccattagcgg
    10921 tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggcggtat ttaatttctt
    10981 cgtgacgtcc ggttctggtc aggcggcgtt aaccatgccg ttactggcac cgcttggcga
    11041 tctggtcggt gttaaccgtc aggttaccgt gctggcattc cagtttggtg atggcttcag
    11101 tcacatcatt tacccaacct cagcttcgtt aatggcaact ctcggtgtgt gcagggtgga
    11161 cttccgtaac tggctgaagg tgggcgcgac cctacttgga ctgctgttta ttatgtccag
    11221 cgtcgtagtg atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt
    11281 gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt
    11341 catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat
    11401 ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtaggg cgaagggacc
    11461 gtgagagacg cagctgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa
    11521 ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg
    11581 cgcggattcc agcagttgct catcggcctg gaccacaccg cctgcggtcg catctaacat
    11641 gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc
    11701 atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct
    11761 ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa
    11821 acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga
    11881 gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc
    11941 gattacacta agtacgtcat tatccagcat gccataactc gccggattac cgcctttcgg
    12001 aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag
    12061 atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc
    12121 aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg
    12181 cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt tgccgcgcac
    12241 agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc
    12301 ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg
    12361 ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg acccatgaat
    12421 gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata
    12481 ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga
    12541 cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc
    12601 catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac
    12661 caaataaatt cggctgaccc agtgctaaca aatgttctgc catcgcgaac agtttctctg
    12721 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    12781 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    12841 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    12901 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    12961 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    13021 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg
    13081 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    13141 tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact
    13201 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    13261 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    13321 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    13381 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    13441 aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg agtctggtgc
    13501 cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg
    13561 cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca
    13621 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    13681 ggttgaaact cagcgtcttt accatgtact gaacaagcgg ctggaaaact cgccctggct
    13741 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    13801 tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    13861 ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga
    13921 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    13981 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc
    14041 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    14101 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    14161 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta
    14221 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    14281 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    14341 cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    14401 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg gtagtgacgc
    14461 gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg caagggctag
    14521 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    14581 ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    14641 cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta ctcatttccg
    14701 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    14761 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    14821 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    14881 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg
    14941 gtcggcagta tcttgcctgg attcatatcg atgatatggt caacggcatt ctctggctgc
    15001 tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccgata cgcaatgaac
    15061 aatttgccca tgcgctcggt catgcgctgc atcgcccggc tattttgcgc gtccccgcaa
    15121 ccgccattcg gctgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc
    15181 tgcctaaacg gctggaagaa gcgggttttg cgtttcgttg gtacgattta gaagaggcgc
    15241 tggcggatgt cgttcgctga tgtggtttac tgcaaacatc cgccagttaa ctcccggtgt
    15301 tacagggtta gtggctttgc gctataagat cgtctggcga gagtcgggtc accatcataa
    15361 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgc
    15421 gtttgcccag aattaatcca tcattccgcc cttcttcacg taatctgtca gcaatggtca
    15481 tcagtttctc cttttcttgt ggtgcgcgtt ctgctatctc accaataaat gcacggaaac
    15541 gctgggcatc tcccgtttgt aatacataat taaacagggc ttttagctgt ctgtcattag
    15601 tgttccccgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    15661 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    15721 ccggcaccac ggtaatatcc accaacggaa aagccgatga atatatttta cgggcaatcg
    15781 caggctcagc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    15841 gataaaacag catcgggatc accaatggaa gctctttata gcccgcatca agatggtttt
    15901 gcattgccgc aatggaataa cgcatcatgc gaaaagccat taattcttcc ggcttacttt
    15961 ggtgctctat cactacataa atataaccca ctccctcctg cgttttcaca gaccacaaga
    16021 ggtcggaata atattgccgc aggtcgtcat caataaaact gtttggttcc agtttaagcg
    16081 tcgttaaatc acacagtttg cgcagcggcg cgggaagatg aatatcaata aaatcccgcg
    16141 cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg
    16201 atattgtcat ggcgttccgt cacccctcaa atttaagatg acgcgacaat aaccacgatt
    16261 aacacccaat gcagcaggca gtttttatct ttggacagcg aattcagaag tatttttagc
    16321 ggactgcaac ttttacattt cagcggaagc tgactcgcaa attccccatg aaaatactcc
    16381 ggcagcgtta gccaccggag aggaaattat ttcagcgatc ccttaaggaa ctgttgcaga
    16441 cgagggcttt gcgggttgcc aaataactgc tccggcgcgc cctcttcttc tattttcccc
    16501 tgatggagga aaatgacgtg agtagaaaca tggcgagcaa agcccatttc gtgagtcact
    16561 accaccatgg ttttcccctc ttctgccagt tgctgcataa tacgtaacac ttcgcctacc
    16621 agttcaggat cgagcgccga ggtaggttca tcaaacagta aaacttccgg ttccatcgcc
    16681 agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg aaagatgcac cggatatttc
    16741 ccctgcgcac gttcgtctat cccgactttt gccagatact tcaccgcccg ctcgcgcgct
    16801 tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc cagcaccgtc
    16861 atatggctcc agagattgaa gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc
    16921 agttgatttt tatcggcgac tttgagttga ccgtctttgt cgcgcaccag attgatcgtc
    16981 tggccattga ccacgatcga cccttcactc ggtttttcga ggaagttaat gcagcgcaga
    17041 aaggtacttt tccccgatcc cgacgatccg atgatgctta ttacatctcc ggcattcgct
    17101 tgcagtgaga cccctttcag cacttcatgt tcgccgtagc gtttgtgcaa atcgataacg
    17161 tttaatttat tctcggacat cgtattctca gtgcgttgaa gaaggtttca catgctgcaa
    17221 ccagcgtttt tccgctctgc gaaagaggct gatcaggaca taagagatga ttaaatagag
    17281 caccgcggca atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa tatcgcgggc
    17341 tattttcagc agatccggca cagtggccgt aaacgccagc gcagtagagt gcagcatcag
    17401 gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca aaataatgca
    17461 acgatacatt ttaaaagtcg agaagccata ggctctggcg gcttcaattt ccccatgcgg
    17521 aaccgaacgg attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag
    17581 cgccagcacg gtacagttca ggccactgcg gaagaaagcg ttaaggaatt cggttccctt
    17641 aacaatctca agcgtgtaca tgccggaata gaacaccagc aactgaacat acagcggcgt
    17701 accgcgaaaa atataggtaa ataaccagat tggaaactgg atgtatttat tactggagac
    17761 acgaccaatc gccagaaaca gcgccaggac tccgcctatc actaccgaca aaataagcag
    17821 ccacagagtg atcgccacac cagtaaagcg ataaccgtcg gtccacagca gcggtttcca
    17881 gtattcatgt aagatttcga tcacaggtca gccctcttca cacccacgga gtagcggcgc
    17941 tcaaggaaca gcagcacacc attggaaaca gtggtgaaaa ccaggtaaat cacgccacag
    18001 acgatggcga aatagaacgg ttcccaggta cttttgcctg ccagttgggt ggctttgacc
    18061 acatcttcca ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac ctgccagttg
    18121 ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa acatgatccg ccgaaacact
    18181 tgcccacgag taaaaccgaa cgccgtcgcc gcctctatat gccctttcgg cactgccata
    18241 aacgcgccac ggaacgtttc ggtataataa gcaccgtaga taaaaccgag agtaataata
    18301 ccagcgacca tcggatcgat atcaatctgc ccgacgccca tcgcctccgt caccgtgttt
    18361 agtgcaatct gcaaaccgta gaaaatcagc agcatcaaca ccaagtccgg cacgcctcga
    18421 atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc gattttgcga gagcttaccg
    18481 ccagcgccaa ttaaaccgat gattacagcg agcactacag aactgatagc cagctccagc
    18541 gtgacgagcg caccctgtaa aataacacct gaaaacccat acaacatgct gcctgtcctg
    18601 tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt acccgaaggg agcgggcttt
    18661 ctttcaccga tggcgattaa ccgccataaa catcaaaatc gaagtacttt ttcgctaatt
    18721 tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt gttcagtgcc tcgcgcagtt
    18781 cgttatcttc tttacgcagg cccatgccgg tccctacgcc aaacagtttt tcatctttaa
    18841 cagacgggcc accgaatttg taatctttac cgacaggttg tttgaggaaa ccttcgctgg
    18901 cagcgacctc atcctggaac gcggcatcaa tacgtccggc agtcaggtca gaataaatgt
    18961 tgtcctgccc ctgatacgag acgatttcaa tgccttttgg tgcccaatgt tcattaccga
    19021 acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc gactcgactg
    19081 tcggctgaat gtcggaattt ttcgccacca ccaaacgaga atcggcagcg tacagtttgt
    19141 cggtgaaggc tatttcttgc tgacgttttt ccgtaatgga aagcgatgac atgatggcgt
    19201 caatcttctt cgcttttaag gacgggatta acgcatccag cggattttcg acaaacgtac
    19261 attgcgtatt gatgcgtttg cataattcct ttgccagatc gatatcgaag ccaaccagtt
    19321 cgccttgtga attttttgat tcaaatggcg cataggtcgg gtcggtaccg atgcggatgt
    19381 tttgcggaat cgcagcaaac gccgcagttg cgctggagaa ggccagaacc agagagagcg
    19441 atagcaccag ttttttcata atgtcctcaa ctgacagtct ttttataggg aatatttaca
    19501 ggtttgttac acttatcgtg ccataaaatt gaccatcaag gcaaaatatc ccgccccgtt
    19561 agacgtatta tttttcatta aaaatgctta agtatgcatt tcaatgcacc acgatagtgc
    19621 aacaaacctg gcgtgcacca tgatgatcat tccatcaggt acagcttccc agcgacgtat
    19681 cagtcaccgt agacattaaa gtcgaaatac tttttcgcca tcttgtcgta ggtgccgtcc
    19741 tgacgcagct cgccaagcgc cttattgaag gcagccgtca gttcagcatc atctttacgt
    19801 agccctacac cggtgccatc accgaagtat tttttgtctt ttactgatga gccagcaaag
    19861 gcgaaatctt taccagcagg ttgcttgagg aatccttcgc tggcagcaac ttcatcttgt
    19921 aacgcagcat ccagacgtcc tgcagccaga tcggaataga ccaaatcctg gttggcatag
    19981 gccaccacat ccacgccttt gctacgccag gtctcgttag cgtaagcttc ctgggttgat
    20041 ccctgcagca caccaacatg tttacctttc agtgaatcca gcgttggctg aatcggtgaa
    20101 cctttggcag caatcaaacg agaatctgcg gcgtacagct tgtcggagaa ggcaatctcc
    20161 tgctgacgtt tatcggtaat ggaaagcgac gaaataatgg cgtcgatttt tttcgctttc
    20221 agtgagggga tcagcgcgtc aaagtcactg gcaacccagg tacatttcac ctgcatccgt
    20281 ttgcacatct cgttaccgag atcgatatca aagccaacaa aatcaccttt agcatctttc
    20341 gacgagaacg gcgcataggt ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca
    20401 tagctggaag ccgctgcgga gagaccgact aacaaagaca gagcgagaat cgacttcttc
    20461 atacataacc ctcaagtgaa aatggctttt ttatgttgtg tattgtgttg tgtgtttgca
    20521 ggctctttca tgcaggtctt atgccatctt gacgacaaca gtaacattca acgttaaata
    20581 tgttaataag acgttgcatt attgtcctga agttgaagat agcaggtata gcggttgaat
    20641 cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg atttgatcgc ggttgcaaaa
    20701 ttgccctgaa acagggcaac agcggagtta tgcgccctgc cagcgggcaa agagatcttc
    20761 aggaagggtt attgcaaatt ggtcaagaac acgattaacc gtctgattta tcacatcatc
    20821 aagggattgc gggcgatgat aaaacgccgg aacgggaggc ataatcaccg caccgatttc
    20881 tgccgcctga gtcattaaac gcagatggcc taagtgcaat ggtgtttcac gcacgcagag
    20941 caccaacggg cgacgctctt tcagcaccac atctgccgca cgggtcagta agccatcagt
    21001 atagctatgg acaatgccgg aaagggtttt gattgaacag ggtaaaatca ccatccccag
    21061 cgtctggaaa gaaccggaag agatgctggc ggaaatatcg cgcgcatcgt gcgtgacatc
    21121 agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg agggataagg tctggcgcgc
    21181 cgcctggctc atcaccagat gcgtttcgat atctgtgaca tcgcgcagaa cctgtaataa
    21241 gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct acaatgagtc gtttcataaa
    21301 tagttgccct gtcagacttg caggcagact ttgcagtatt tcggcgggag ttgcaagtca
    21361 gggtgccaga ccggcaccct cagcgaaggc atcatccttc gttatgcatt tcgagatttt
    21421 ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt acgtaacgta tcgaggaaat
    21481 cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc gttgaacacc gagcattcaa
    21541 actgctggat atccggattt tcagcgcgaa cggcgtcgat cagatcgttc agatcctgga
    21601 aaatcaaccc gtcagcaccg atgatctggc gaatttcatc aacttcgcga ccgtgagcga
    21661 tcagttccgt ggcgctcggc atatcaatac cataaacgtt cgggaagcga atttccggtg
    21721 ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg tgccatctcg ataatctgct
    21781 cagaagtggt gccacggacg atggagtcgt cgaccagcag gacgttttta tcgcggaact
    21841 cggcgcggtt ggcattcagt ttacggcgca cggacttacg acgcagctgc tggcccggca
    21901 tgataaaggt gcggccaaca tagcggtttt taacgaagcc ctggcggtac ggtttgccca
    21961 gaatacgagc aatttccagc gcgatatcac acgaggtttc tgggatcggg atcaccacgt
    22021 cgatatccag atcttcccat tcgcgggcaa ttttctcgcc cagtttcgtg cccatattca
    22081 cacgcgcgct gtaaacggaa attttgtcga tgaacgagtc cgggcgagca aagtatacat
    22141 actcaaacag gcacggattg ctgaccggat tgtcagcaca ttgacgggta aacaactgcc
    22201 cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca
    22261 gcgtatcgag cgctacgctt tcggaagcga ccatatattc tgtacggttc tcgtcaatat
    22321 cacgttttcc cagtaccagc ggacgaatcc cgtttggatc gcggaaagca accataccgt
    22381 ggccgataat catcgccaca caggcatatg cgccgcggat taagcggttt gtggcagcaa
    22441 tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg gaagttgtcc agctcgctag
    22501 cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt
    22561 caaacagttt tttacgcaac tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa
    22621 tgccatacgg agagttaacg taaaacggct gcgcttcaga ggcgctggag ctgccagccg
    22681 tagggtaacg cacatgacca atgcccatat tgccctgcaa acgctgcata tggcgagctt
    22741 caaatacatc gctcaccagc ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta
    22801 tggtgatgat gccggcggca tcctgaccgc gatgctgaag caccgttaag gcatcataaa
    22861 tcgactggtt aaccggcata acaccggcga taccgacaat accgcacata cgtctttttc
    22921 ctcgttaagc cacatctcag agcacttacg ctctgggcaa gaaacttgac gagctttgca
    22981 gataatcaaa aaaccatctg atgataaaac tgaactgcgg gatcagctgt gatttgctcc
    23041 agtcttcgct tttcgacacc ccggtaaagg agtcgagaaa gaagagaatg gcggcaacaa
    23101 tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc
    23161 ccgttttctc caccaactgg cctatcacga agttcacgat agcaccaacg atcagggtag
    23221 cgataaacag taccgcgatg gcaattccat ttcgaaccag ttcgtcttca aagcccgtaa
    23281 accagactga caggtaagtg tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca
    23341 ccagcgataa cgcttcacga acaaagccgc ggatcaggct aaccagagag gaaaaagcaa
    23401 tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt ctcacgattt tacgccctgt
    23461 catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc
    23521 cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc catcgacttt ttatgccttt
    23581 gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc ttatccgacc tacggttcta
    23641 acactgccca ggcctgataa gacgcgccag cgtcgcatca ggcaagactg tattaattcg
    23701 gcgtatagcc cattaccacg ccacttaagc cagaaagttg cttcaactca cccagcgaac
    23761 ctttcagctt atctttcgag gcatccggcc caaccagaat acgggtaatt ttaccctgca
    23821 ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta ccgacaatct
    23881 cattcacttt atcggcattt ttcagcgcac ccagttgcac aacataggct ttacccgttg
    23941 gcgctgcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt ggtgcttcaa
    24001 ccttcggttt aggcggctcc accggtttcg gcttcggtgg ggcgaccggt gcaggttccg
    24061 gttcaaactc ggtgttattg gctgcaatag tggtcggatc gagcgacggt gctgcggcat
    24121 cacctgcccg cacctcttcc gctgcgcctt ccggcggctg cgtcggcaac gcctgggtgg
    24181 cggctggcat catatcaggc tcatcgcgat cgcccgcttt cggcaccagc gggatagccg
    24241 cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt acaatcaccc
    24301 ccagcgccac cagcacgatc gtgcccacta accgattctg aaacttactt gccaccgctt
    24361 ctcctcgcgt caatcacttc catgacatgt gcgacagtgt ggaaagaacc acacaccagc
    24421 acggtgtctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc aacgctatca
    24481 aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc gcgcggccct
    24541 tccagtggcg cacaatacca gtcatcaacc acgcttttca accaggccag agttccggca
    24601 atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt cggtagcgct
    24661 ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc aaaaataacg
    24721 cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc aatcccgtcg
    24781 cgaatggcat tttcactgac ttccagcccg ctggcacgca gtgccgccag cgccgttgcg
    24841 gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg agcatcgcta
    24901 aacgcccagt catgatcggt gacggaatag ttccactcaa cgccccgacg ttgtaacagt
    24961 gcaccttttt cctgcgccac atcagcaatg gtagaaggca tttccggctc accgacaatt
    25021 gccggttttg cgctgcgaaa gatgcctgct ttctcgcgac caatactttc gcgatctggt
    25081 cccagccagt cggtatgatc cagcgcaatg ctggttacta cagccacatc ggcatcgaca
    25141 atattggttg cgtccagacg accgcccagc cctacttcca gaatcactac gtcaagttgt
    25201 gcctgtttga acagccacaa cgccgacagc gtaccgtact cgaaataggt cagggaaata
    25261 tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc cggcaattcc
    25321 tgcccctgca cacgtacgcg ctcggtataa cgcaccagat gcggtgaact gtagacgccc
    25381 actttgtacc ctgccgccat cagaatcgac tccagcgtac ggcaggtggt gcctttgcca
    25441 ttcgtacccg caacggtaaa cacaaatggc gctggtttca ggacgccaag acgcgccgcg
    25501 acctggctca cgcgctcaag gccgagatcg atagttttac tgtgcaggtt ttccagataa
    25561 gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat catggtatcc
    25621 gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc ccttatcagt
    25681 tatcaggcct caggttcctg atccggtacc gggggtacca ctacgccttc acgcggcgct
    25741 tcaggattcg gcgctggcag attcatcaac ttcgccagaa tgctcgccag tttcaggcgc
    25801 atttccggac gacggacgat catgtcgatg gcgcctttct cgatcaggaa ttcactgcgc
    25861 tggaatcctg gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg accggcaaag
    25921 ccgattaacg ctttaggttc agcgatgttg agatcgccca gcatggcgaa acttgcagaa
    25981 acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg gcaagccgcg ctcctgcatt
    26041 tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc ttcctgcata
    26101 cgtgcgccac cagaggcgga gaagcagatc agcgggcagt tatcttccag cgcctgctca
    26161 acggcacgca cgaaacgtgc acccacaaca gaccccattg aaccgcccat aaaggcgaac
    26221 tcgaatgccg cagcgacaac cggcattcca tacagagtgc ctttcatcac caccagcgca
    26281 tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt cttggagtca
    26341 cgaaacttca gcacatcttt cggctcaagc tcgctaccca gctccacaag gcttccttca
    26401 tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc acacttcgga
    26461 cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca gctatcacac
    26521 ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt gcttttaatt
    26581 cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc cagttttatc
    26641 tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc acattaaaac
    26701 ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cggagttact ttttattttg
    26761 cggcgcgtgc cgcagcgcgt ttgtgacgaa taaattcgaa aaccccaggc aatattgaaa
    26821 caaaaataat gccaacaatc atcagcttaa ggttgctctg aatgaagggg agcgtgccga
    26881 agaaataacc cgcgtaggta aaaatcagta cccacaacag tgcgccgatc acgttataag
    26941 cggcgaaatg acggtacgac atgtggccca ttcccgcaac aaacggcgcg aacgttctga
    27001 cgatcggtac aaaacgggcg agaataatcg ttttgccgcc atgtctctcg taaaactgat
    27061 gagtcttatc aagataactg cgacgaaaga ttttcgaatc cgggttactg aacagtttgt
    27121 caccaaataa ccgtccaatg gtatagttca ctgcatcacc gacaatagcg gcaatcaaca
    27181 taagcaccac catcatatgg acattgagat cgttagtttc cagcgacgcc aatgcaccag
    27241 caacaaacag cagcgaatca ccaggcagga atggcgttac caccagcccc gtttcgcaga
    27301 acagaatcaa aaacagaatg gcataaaccc agacgccgta ctccgcgacc agctctgcca
    27361 gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc atatttgcct
    27421 atgccttgta ctcgtcatct ttcaggctgt aactacgttg gctacagcct gaaatgttcc
    27481 gagcattatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt tggaagatca
    27541 taccggtcag ggtaatccac cgcgaccaga tacagccctt ccgcttttgc cgttgctgcc
    27601 gccagcgttc tgtcctttgc cgccagcaac tctgctatcc agctctccgg ctggttgtgg
    27661 gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac aaaggcattc
    27721 gctttgatat ctaccaccac ataaggaccg tgacgcgtga cattaatatg cataacgttg
    27781 cgccacgggg ttcgggactg gcactgcacc gcacggaacg aggtgaaatc attctcgccc
    27841 agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta aaaatgggtt
    27901 acccctttac tcagtaccgc cgggcgcagc cgatgattgt agatgatgta gcgataacgg
    27961 cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac ccaacgcacg
    28021 gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc acgccgcgtc tttgcgctgc
    28081 gcggtcgttt cgaaatgcac tacctgcccg gtaccgtgta caccagcgtc agtacgcccg
    28141 gcgcagaaga cggtaatggg ttcgttcgcc acctgggaga gcgccttttc cagcttctcc
    28201 tgcacactgc ggacttcatt ctgccgttgc cagccgtaat acttactgcc gtcgtactca
    28261 atgcccagcg caattttata aactggcggt tgttgctggt ccgacattag tacagatact
    28321 cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag cgaacgttat
    28381 cggccaccga ccagaactgg acttgctccg gcataccgta gtcattacgc acgcagccaa
    28441 cagaaagatg cggcgtaccc gaagcatccc ctacctgagt cgggaattcg ttctcttcag
    28501 agagcacaat atcttcgccc tgagcaaacg catcacacgc ttcttctgcc gccagcggac
    28561 gcagtgcttc aaagttgacc atctgggcat gaccgtagaa taccggtgcc tggacgacgc
    28621 ttgccgaaat catcagccct tcgtcctgca ggattttgcg tacttcgtca acgatacgac
    28681 gttcttcacg cacgctacct tcgctatccg gcaacaacgg cagcatattg aacgccagtt
    28741 gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt gagcaatttc gcactctgcc
    28801 ccgctaacgc atcgaccgct tttttgccct gggcggaggc tgaaatcagg ctggtaacac
    28861 tgatacgcga caaaccgccc tgatcgatta acggtttcag tgccgccagc agctggctgg
    28921 tcagactgtc tggtacggcg atgacattcc ggttgcggta atcggtcagt acaaacgggt
    28981 ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacagtccg ctgctgtcga
    29041 tcaccaggca acctgagttg gtcgcttctt caacccaggc agcggtagct tctttgcctg
    29101 cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc agcggcatcc tgcacggtga
    29161 ttgtcttacc accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc agtgcataaa
    29221 tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg cccacagcgc
    29281 cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac tccagaaata
    29341 gcaaagctcc ctgccaatgt tacagcagag agcatgaaga agagattaac gtgccggatg
    29401 atgaacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt catcacaaat
    29461 tacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagtt tatcgaactc
    29521 acccggtatc ccggcgactt tacgcagctg tgcgtcatcg cggcgcacat catacaccaa
    29581 atgcaccagc cttttcagcg tcggttgatc gagcgggcca tgcagcgtaa tgcgaccaaa
    29641 ctctggcgcg ggcagtaatg tatccagcgc aacgtgctgt tcatgcccaa taaacttgct
    29701 ataggcttca aacacttgcg tagtaccgcg tgctttacct tccagggtat aacctgcgat
    29761 atgcggcgtg ccgatatcca cttttgtcag caactccacg ttaagttccg gttcgccttc
    29821 ccagacatcc agcactacgc ttaacttctg gccttcattc aggcaggtca gcaacgcagt
    29881 attatcgacg actgcgccac ggcaggcgtt aatcagaatc gctcccggct tcaggctacg
    29941 gatcagtttt tcatccgcca gatgcagcgt tttgtacggc ccatctttga agagtggcgt
    30001 atggaaagtc agaatatccg catgctgaac taattcatcc agcgagcgga aatcaccctc
    30061 atccccacgg tcagcgcgag gcggatcgca aagcaaggtt ttaatcccta acgcttccag
    30121 tcgcgcttgc aatcgacgtc caacgttacc aacgcccacg atccccaccg tacggtcgtg
    30181 cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat attccaccac
    30241 cgcaatcgca ttacagccag gtgccgcgga aaaaccaatt cccgcctgct ttaaccatgc
    30301 ttcattgaca tggtcggtcc ctgctgtggc agtaccaaca aatttaatgg gttttcctgc
    30361 cagcaaagat tcattcactt tcgtgaccga acgcaccatc agcgcatccg cgtctgccag
    30421 ttgagcgacg gggattggac gcccgggaac cgcggtcacc tcacccaaac ggctaaataa
    30481 gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt tacctgtatg
    30541 agacgagagt taaccggaca agtgtgccat aatctcgcgg ccaggcatac ttgcgaagat
    30601 ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctcct gggcaaccac
    30661 caggtcaggg agataacctg ccgtctggcg cgggcaatca gcctttatcc agtcagcaac
    30721 gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacag caaagagcag
    30781 aactgtgggc gggtatcagg cacgatattg gtctgtcggg agattcaccg ctgctttcgc
    30841 gtcacttccc tgccgctgaa cataatctgg cgcaacgtct gctggccgcg caaaaaagcc
    30901 attctgcccg ccagctttta gcgcaattag gcgagtattt acgtctgggg aataatcgtc
    30961 aggcggtcac ggattatatc cgtcataact ttggtcagac gccgctgaat cagctctcac
    31021 cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt attccgcaac
    31081 cacagcagcg cgaggcgacc gaccgtcctt tattaccggc ggagcacaat gcgctaaaac
    31141 agctggtgac caaacttgcg gcggcaacgg gggaacccag caaacagatc tggcaatcga
    31201 tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg tttaaccatc
    31261 tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgccg acgctggaat
    31321 cactacagat gacgctaaaa caacctttag atgccagtga actggcggcg ttatcggcat
    31381 atatccagca aaaatatggt ctttctgcgc aatcatcgct ttcttctgcc caggccgagg
    31441 atattcttaa tcagctttat caacggcggg ttaaagggat tgatccgcgt gttatgcaac
    31501 cgctgcttaa tccttttcca ccgatgatag acacgttgca aaatatggca acgcgtcccg
    31561 cgctgtggat actgttagtc gcgattatcc tgatgctggt ctggctggtt cgttaacccc
    31621 gacgaaatga cagtatcgtg acaataatac ccagcaccgc agagatcgcc ccggcaagaa
    31681 ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc cagcggcccg gagacgccga
    31741 gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaact tgtgagggga
    31801 cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg gctccggtta
    31861 acgccgcgcc cgctaatgcg acccatgcac ctggggcttg ccagagcagc aacaagccca
    31921 ccgtttctac aagcagagag acaatcgcca ctttcacgcc gccaaaacgg tccggcatcc
    31981 agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga gtaaagcccg
    32041 ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata acagcaaaac
    32101 caacgccttg tagtgccaga cctaaccctg gtttccagat aagcccgaca acgctccaca
    32161 gcgatggacg ttctcccgcc agggccggta ctttgcgcac tgtgccgtta caggcccacg
    32221 ccagtacggg taataccatt gtggtgatcg ccagtgcggc aaaaccgtaa tggctatgaa
    32281 tcaacaggcc aagcggagca ccaacagcga gggcaccgta aatcgccatt ccgttccatg
    32341 acatcacttt gccagagtgt tttggcccta cgatgcctag tccccaggtc agagcgcctg
    32401 tcagtaactg gctttcacca aagccaagaa tcaaacgccc gacgaccaac agagcaaatt
    32461 tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa cgcgccgcca gccagaccac
    32521 aagctaacat tccctgaagc gccgaacgtt ttgcaccata ttgatcggcc agtcgcccgg
    32581 cgtaaccacg cgtcagcacc gtagccagaa actgaatccc gacggcaatg ccgaccatgg
    32641 tattgccata gcccagttca tgatgaacaa acagcgggat aaccggcaac ggcaacccta
    32701 cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa gagcgaaaaa ttggcagaag
    32761 atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag cgcaaggcga
    32821 ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca ggatttaacg
    32881 cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt tgcctggaac tggcggcgat
    32941 tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt gcgttagtgc cggaggccgc
    33001 attggcgcgt aacgtcggat gcgacgctgg cgcgtctact ccgacctact gcgaattaat
    33061 ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag ctgttagaca
    33121 taacggtggt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc gcagcctgct
    33181 cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc atcagcagag
    33241 agtagatagc ttcctgtacg ccagcagcgc ccagagagtg accggtcatg gctttggttg
    33301 cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagc tctttcacgt
    33361 cgccaaccgg agtcgaagta ccgtgggagt tcaggtaatc gattggggta tcaacgccat
    33421 gcatcgccat cttcatgcag cgtactgcgc cttcgccaga cggagcaacc atgtctgcac
    33481 catcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca cgcgccagcg
    33541 cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg aaaccgtcac
    33601 ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta gtagacagcg
    33661 cacccattgc gtcgaattcg caagccattt cccagcacag ctcttcgccg ccgccagcaa
    33721 acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata cagtgtgcgg
    33781 aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac ggggtggcga
    33841 ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca accgctttca
    33901 ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg gaaacgcggg gagccgccgc
    33961 cggaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga gagaggcccg
    34021 catcagcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg ctcataaagc
    34081 gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc cagacgtggc
    34141 tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt ccttcacgca
    34201 gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc aggccagtaa
    34261 tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt tcgaatgcac
    34321 aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag aaatttgcgc
    34381 agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac ttacgtgaaa
    34441 cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc tgtttcccga
    34501 gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg ttatgttttt
    34561 ctgggaggca accaattaga ggtacgcttt cctgagcatc cacatcctct gtttgtggta
    34621 gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc atttgatcag
    34681 tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag ttttgagaaa
    34741 tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga actggctccg
    34801 tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca tcgtttattg
    34861 ctcgatgcag gccgcgtgac gctggattta tggtttggcg atattaacga actgaccagc
    34921 caactggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg ctttgcgcca
    34981 gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag gttggcgcgt
    35041 ccgggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg tttgcaggac
    35101 gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat gctttgcggg
    35161 gtgatggaac agacattacc gctcccctgc tccgcgccgt ggtttaaccg cacgggcagc
    35221 agcaaacggg aagcggcgat tatcggcggt ggtattgcca gcgcgttgtt gtcgctggcg
    35281 ctattacggc gcggctggca ggtaacgctt tattgcgcgg atgaggcccc cgcactgggt
    35341 gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga tgaggcgcta
    35401 aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca attacccgtt
    35461 aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa aagccagcat
    35521 aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc cgttgaggca
    35581 aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac ttatcctcaa
    35641 ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc gcaacagcag
    35701 ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga tgactgttgg
    35761 ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact ggcgaacggg
    35821 catcaaatca gccgtttcag ccaaacgtcg actctcccgg tgtattcggt tgccgggcag
    35881 gtcagccata ttccgacaac gccggaactg gcagagctga agcaggtgct gtgctatgac
    35941 ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc cagttatcat
    36001 cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg ccagcggttg
    36061 attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga taaagaggcg
    36121 cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa tgttcccgat
    36181 tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc agaaagatga ggcggtaagc
    36241 gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg tttgtgttct
    36301 gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat tccgatggat
    36361 gccagtacgc tggcggcgtt aaacccgaat cggttatggg tgcggaaatt gttgaagggt
    36421 aaagcggtta aggcggggta atctgctctc gcagtgtttg ccggatgcgg cgtgaacgcc
    36481 ttatccgacc tacgtgtgac gtgtaggcct gataagacgc gataagcgtc gcatcaggca
    36541 tggtgctcca gacgccgcaa cttactgctg tgacgcctgc tgaaacaggt tttcccacat
    36601 atcggtcacc agcgcctggt cacgcggcga caattcaccg gcaccaatgg ctttttccag
    36661 actctggcta acggtcgtat gcaccgcctg agcggagtgg tcatcaccac tttccagttc
    36721 tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact catcatcact
    36781 ggcatggtca accataccgt cgattaatgc cagaatgcgt gattcaaact ccgcgatcat
    36841 cttctttcct cattgtaaaa cgtggcaccg gcttcagttg agcgcttccg gccacgggaa
    36901 ctgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa tgaagcgtgc
    36961 cgggcgttca ggaatgccgt ttgctaggta atccatcacc tgcgcatgta cccgccgttg
    37021 aaacaccaca cgatccggtt cgaagtcgcc ttccagattg tcgcaactga cgttaaacgg
    37081 atatcccgcc gccacacaga acaaccagtc gaacgcctgc ggcttcactt caacatcttc
    37141 aaactggctt tgcgtttggg catcgcgtcc gtccgggcaa taccagtagc cgaaatcaac
    37201 cagctcacgg cgcgctttcc cggcaataca ccagtgcgaa atctcatgaa ttgcgctggc
    37261 ataaaagcca tgagcaaaga cgattcggtt atacggtact tccgcatcag caggaagata
    37321 gatcggttcg tcgtcgcctt taatcagacg ggtattaaat tcatcggcaa agcagctatt
    37381 aaaaatttca atcaactgct catagtggtg tgtactgttc attagttcat ccccaaccag
    37441 tggaggatct cctgtccgtg gctatcaaaa agtaatttgg cactcatcac cgctgagaca
    37501 ataacaatca tcgggcggat cagcttttgt cctttgctca acaccagtcg tgaacccatg
    37561 cgcgcgccga ggaactgccc caccagcatg acaaagcccg tcgcccaaat cactttgcca
    37621 ccgagaataa acagcagtaa accgccgatg tttgacgttg cgttgagtaa tttggcgtgc
    37681 gccgtggctt tggcgaggtt gaatccgcac agcgtaacaa aggccagtgc gtaaaacgat
    37741 ccggctgccg gaccaaagaa tccgtcataa aaaccgacgc agccaccggc aattaacgcg
    37801 aagggcaaac cgtacatccg gcgctggcga tcttcttcac ccagctttgg catcagcaag
    37861 aaatagaggc cgatacaaat caccagaatg ggcaaaatct gccgtaagac atcagcctga
    37921 acgtattgca ccagcagtgc gccgctcatt gagccgataa aggtcatggc gatattgagt
    37981 ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc ggaaatagag
    38041 ccgccgcagg cttgcagttt attggttgcc agcgcattag cgggagacat ccctgccgcc
    38101 atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat aaatcccgcc
    38161 agcatggcga caaaaaagag aacacccagc aacagcgggg aaaccataaa caggctatta
    38221 aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc ggcaacggag
    38281 gcggtgtctt cttctcaggc tttgttgttc ccggttttgg aggttcaaac cagctttgca
    38341 gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg atcttcacac tccagactat
    38401 cggcaggaca acgtaatcgt acatgcatat gcgcacgatg ctggaaccag ggtcgcactt
    38461 tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta atcgccggat
    38521 taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag tttgatcaag ctgaaaattt
    38581 ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg ggaaactaag tccagtgctt
    38641 gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg cagttgcaga aagatatcga
    38701 catccagtcc ggtctggtgg ctggcatgac cgccgttgaa acacccacca gcgggcatcc
    38761 ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcta ctcagacgct
    38821 ggataaacat caccagatcc gggtgaccga aatagcgacg ctgatcggta cgcatgacct
    38881 gataatgttc ggactgtatc ggcaacgtgt cagcgccgac aatacagcca ttagaaaaac
    38941 tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac ggcgtcgctg
    39001 ccaggctagc gctactggca agcagagcca gcagcgcaat cgcggttttg ttcatttttt
    39061 accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt aacaggtgat
    39121 ccattaaaac gatcgccagc atcgcttcag cgatcggcac tgcacggatc ccgacacagg
    39181 gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggtta atagtgcgcc
    39241 ccggcacggt aatgctggag gtcggtttca gcgccatatg agcaatgatt tgctgcccgc
    39301 tgctgatacc gccgagaatg ccgcccgcat ggttgctctg gaaaccgtct ttggtgattt
    39361 cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa accgtcgcca atttccacgc
    39421 ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg cggtcaaaga
    39481 ccggctcgcc aagtccggcg gggacaccac tggcaacaac ggtgactttc gcgccgatgg
    39541 agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt ttgtccgggt
    39601 ccgggcagaa gaacggattt tgttcaacca gcgaccagtc tttgatttcc agcggaatgt
    39661 cgcccatctg ggtcaagcag ccgcggattt caataccaaa tttctcggcg agatattttt
    39721 tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa cgtccaccgc
    39781 cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcagcatgg cctgggcgga
    39841 aaacgtcttt aattgcgctg taatcctgag agcgctggtc agtgttttcg atcaacaagc
    39901 caatgctggt gccagtagta acgccttcaa aaacaccgga gagaattttg acctgatccg
    39961 gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg acgacggtcg aggtcatgtt
    40021 gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg cagccgagcg
    40081 ccagcccatg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt gtgtttccag
    40141 ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata aatcgcgaaa
    40201 tattcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc gccgccgtta
    40261 tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg taccatgctg
    40321 ttgccgactt cacaaatcaa cacgccatca tcagcaaggt aatctgccgc gtttccaaga
    40381 atgcgacgtg tcagtttaag gccgtcggtg ccagatgcca ggcccagttc cggctcgtga
    40441 cggtattcgt ttggcaggtc ggacatatct tccgcgtcga catacggcgg gttagtgaca
    40501 atcagatcgt attgcacttt cggcaaatca cggaacagat cggaacgaat cggaatgacg
    40561 ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa ccgccagcgc gtcgggagag
    40621 atatctaccg cgtcgacttc cgcttccggg aaggcataag cacaggcaat ggcgatgcag
    40681 ccgctaccgg tacacatatc taaaatatgc tgcggttgct tgctgatgag tccggcaaat
    40741 ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc atcgacgtaa
    40801 aattcatgac cgcagaacca ggctttgttg gtcaggtaag ccaccggaat gcgctcgttg
    40861 acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag acgcgcggtg
    40921 cgcatatctt ccgggatatc cagcggcagg tagagcgatg gtaacaccag ctgtacggct
    40981 tcatcccacg ggttatcggt gccgtgaccg taccagatat ttgccgcgct gaagcggctt
    41041 accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc atcaacgaaa
    41101 attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct agtttgccat
    41161 gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg tttaaaacga
    41221 tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag aaattgagac agataaatga
    41281 aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg atggcgggga
    41341 ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaagaaa attagcgaag
    41401 tgccggtgaa acggttgatt caggagcagg ccgatgccag ccattatttt tccgatgagt
    41461 ttcagccgtt attgaatacc gaaggtccgg tcaaatatgt tcgcccggat gtcagccatt
    41521 ttgaggcgaa gaaactgcgc cgtggcgatt attcaccgga attgtttttg gatttacacg
    41581 gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt gattgccgcc tgtcgccgcg
    41641 aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa gcatattttg aagcaacaaa
    41701 caccgctgtg gctggcacaa catccgcatg tgatggcatt tcaccaggca ccgaaagagt
    41761 acggcggtga tgcggctttg ttggtgttga ttgaagtgga agagtggttg ccgccggagt
    41821 tgccctgaat gctggcagca aatgccggag cagcgtaaac gccttatccg gcctacaaat
    41881 atgtgataat tcaataaatt gcaattcact tcgtaggcct gataagacgc atcagagcaa
    41941 tactacgaac tacagttcat tcaatacata ctgaaaacga cacgtcgaat caatcggttg
    42001 cccactactg gtattcacat tcaccacaat atcgtgaagt actttatccg ttaccggatg
    42061 acgagttggc gtaaccgtta ccgttgattt atcacaaccg taagaggtaa aggttaacaa
    42121 ttctgatacc gcgcggcagt atgctcctct gccatcatta tcagaaaaat tactgccatc
    42181 acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg gtggtcactg ttgaccccgt
    42241 ggactgaatt cgtaacgcct tattatggct gacataattt tcactccagg agatatcgta
    42301 tgaagcgacc ggtatcgttg ggcatattgt gttatgcact gtaggggccg tatagcttcc
    42361 aaatccgggt aaatctgtat aggaataaaa ggatgcgatc caagggcaac cataatcatc
    42421 agatgcaacg gtaattttaa acgtcatata tgcacaacga aaaattttcg tatctatgcc
    42481 agggcaattt gagttattaa ttgtatcaaa gtcagtcagt accaaagttg gagctgaacc
    42541 cgacccagac caggaaataa cccgcaacgt atcgccttta tacgcaaaag aatcatattt
    42601 cccatatgca acctcctttg gcgttatcac cgtaacattg aaaacactgc tcgctacagc
    42661 accggttacc ttgtccatgg cataataaac tctcacactg tcaaaactcg cactcataag
    42721 tttatctcca gcataacttt tagatgcgcc gacaacactc agaacgagca gcattaggct
    42781 cgcgaaaatt atggtccatt ttttcatttc agaaatactc aaaggttgcc gtcactatgg
    42841 cactaaagtc tccggacgtt acatctcgcc cgctctttgc ctgtaaccag gtattaaaat
    42901 ttaggctgtt atttccctga ttcagtggga gcgtgacgcc tgttgtattg ttgattggca
    42961 ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc ggatgctgat gacgaggagt
    43021 caaaggccag aaagccgggt aaagcactat cttctgttcc tgtcaacgtt actttgacat
    43081 taaacgatgt agaactatga caatctttca attgaaacac caatggcact cgctctgact
    43141 gcccaaggtt cattaaatcg ctggcggcaa tggcaggaaa atgtacttct gccaactggc
    43201 taccattaat cactggcgta cacgatttac tgattaaatt gccggtaaag tgcaaattat
    43261 ctcctgctat tgtggttcca ctgcccagca acatcaggct actaataaaa aacaacgtac
    43321 gctttttctt cattggtaat ctacctgtaa cgtggcccag gcctcaaagt ccgcctctgg
    43381 caaaacgaca ccactctttt ttactggcac agccgttaac accggtaaat cggtttcatt
    43441 caccacaagt ggcgtattga tcgtaaaact ctgccctgcc tgtttgagct gaatgcctaa
    43501 accagtgata ttgctgctta ctgcagcatt atcaaaatcg cttggtgtgc cactccagct
    43561 caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg caggtaatgg aatacggcac
    43621 ggtttgctca taattgctgc cattaatgtc atcaataagc acatcctgaa aagttatttc
    43681 aatggtttga gcgttgctaa tcgtgcagtt aggtggctgc actaaataac cgtgaaaaga
    43741 gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa ataagcgaga tccgtttcat
    43801 tggtaatcca ccacaattgt ggcactggcg ttaaattcag ctgccgctaa cgttgtacca
    43861 ctctgtttca ccggcacagc ttccagaacg ggaactcccg aggaaaaatt aaatggcaac
    43921 catgagccag aggttaagtc gagtattgtg tgatcactac ttttctgaac ccgaatacca
    43981 aacccaggaa tccccgtgct gataactgtt tcaccgttaa tcacgaccgt tgtcgcctgc
    44041 atctgtagac gcaggtcatc cattgccaga ttgttacaaa caagcgagta gtcaatggga
    44101 cgtttataat caaccccatt gatcttatta atgaatacat taccaaactc aactgccgca
    44161 ccatttaccg tacaaggagg aggggcatcg acaagcaccc gtaaattcat acttactgac
    44221 ttactcgctg caactgcaaa gccgctacca acacaacata tcagtaaccc agttaccgcc
    44281 cagacagaac aagatccttt catactggct ctcccggcct cagcctttgc ggctctgctc
    44341 ttcatcgacc tggcaactgt taccgttgca agtgaaaaag agcggcatcc gagcgccata
    44401 gtcgttgaca taggtcagta ccggcacgct acccatcttc acattcagcg gtgcagaggt
    44461 tttcggcgac atcactattg gcgagaaacc ggctgccgga ttgccatttt tctgtgtgct
    44521 ggcattactg aaaatgacgt aatacggtgt cgggttattg acggtaaatt cctgaccgtt
    44581 gcgggttaaa gtaaccttat attgccaggg gtttttcatg ctgacgttct ccagcgcctt
    44641 cggtcgccag aacaacttaa tacgcgtttg tagtgctatt tgcagcgtgt tggctttgtt
    44701 tgattttggc gggatctcac gcacgttgaa gtagaacagg ctttcacgat cggcaggcaa
    44761 tttgttgata tccggcattc cctgcacttt tacctgcccg ttcatcattg aatcgatgcg
    44821 ttgcaccggt ggcagaacag ttaacggtga actgatcttg ttaccctttt catcttcgat
    44881 ccagctttgg gccagataag gcagtttcgg atcgttattg cgcaacgtca cgctgattga
    44941 tttgtcgctt tcattaaata tcaaacgggt acgatctggc gttacagaag cctgggcaga
    45001 caaactggca gcgctaagca gtaacgctag cgtcgtagca cccaatttgg cagagcaaag
    45061 taaatcagac atggatcatt ccttaatcac gttattggtt agctgaaaca gaaacagggg
    45121 gattggtggg tgtcacctgt tgcgtctgct cctgaatgac aggctttatg tcatcaggaa
    45181 caaccggcgc gacgttgcct ttatgctggc atggcagtaa caagccgtta aacagatcgg
    45241 caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac accactccag aacacactca
    45301 tgtgttcgcc aggtttcacc ccagccagat aaacattgcc gtcatcatcg acaaggccca
    45361 ctgtctgctc gttatcattt tttacttctg cgccaaacgg aggatggctg ccgtcttgta
    45421 aacgcagcac cgccatcgct ttttgaccac tgatgacggc aaatttgcgg tagccaatcg
    45481 ccccttcagt tagcgtggct tgtaccaccg actgggttgc ttcggcgttt tccggcagtt
    45541 tgttgaggtc gatatacgcc tgattgcggt aatagttatt gacgtcagaa acgacggctt
    45601 taccaaacat attggtataa acagcagcac cgttaccttc taccggaaca tcggcaacgc
    45661 catcggcatc aatcaacaag cgtgtcccgc ccatattctg ggtacggtga agtgcgccac
    45721 cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc cgcagaggtg tattgccctt
    45781 catggtagtt cgcactgagg tcaacctgcg ccagcgaacc atcatggctg taatagccgt
    45841 caacgctggt gtgtttgtca ctggtgccga cgttcaactg atagtgagtc gcgtcatcga
    45901 cacggctgaa ataaccgacc tgactgctgt ccgtcccact gccatagtta ccgttatagc
    45961 taacggtact gttgtcgccc cacggcatac tgagcgaaat gtacatgcct ttgtcggcct
    46021 ggttgtcata ctcgtaacgg tagccagtca tcgagatgct gacattacga atactgccca
    46081 tattgaaata gtgcgagagc atgatgttgt agtttgtctg ctcctcgcga tcccagtagg
    46141 tatggcgggt atagttgaga taaaccgaaa cacccgcatc gcggaagttc tggttataag
    46201 tggcggtgta catctctttg tcgttgcccg tgcgtaccat tccgctgtca ctggcatcca
    46261 gatactcgct catggtcata aagttctctt ccgagaagcg atatccagca aaagtaacgc
    46321 ggctgttgag ctggtcaaag tctttggaat aactcacacg gaaggagtta ccgtccagcg
    46381 aacctttgcc ataagcggtg tctttatcca gtttggtgtg cgagtgagta acatcaaacg
    46441 cgaccgcgcc gaatgtagac aaatcgcgac cgacgccaag cgccgcagac tgatagtttt
    46501 catctcccag tgcgccgcca tataacgacc agccgttagc gatcccccag gaagcttcag
    46561 caccagaaaa aaattcaccc tcgacatggt gtccccactc ttgcggacgg cccatcatga
    46621 tcttatagcg aacctgacct gggcgagtga ggtatggcat cgaggcggtg ctgatgtcat
    46681 attcctgcac ctggccgttc tgttcttcaa tgcgaatatg caacgtaccg gagacagaat
    46741 caccaagatc ctgaatacga aacggtccag ccggaacctg cgtttcgtaa atcacacgcc
    46801 ccatctggct gacggtcact tttgctgtgg tgtgtgccac gccggaaatg tctggcgcgt
    46861 agccgcgcag attgggaggc aacatttgat cgtcagtact gacactgcca ccaacatagt
    46921 taaaaccatc aaaaatatcg gatctgaggt aatcctcgcc cagcgccagt ttggctttta
    46981 atgatggtaa cgcccgccag gcatagtagc gactccactc ccattttttt tgagtttcat
    47041 cgccgctgaa ttcatcgtca tcattactgc gagtatgttg atagttagtc tgccagtcag
    47101 cacgcatacg ccacggcccc aggttaaccc cgaccgtccc gttgccgctg atctcgttac
    47161 tatcatcacc gccattttct tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga
    47221 tcccggagat gccgtcatcc caacgtgaag gcggatccca gtcggggtaa gtatattcga
    47281 ggtaagcctg tggcagtgaa atgactaatg cggactggct taaatcagcc ttaatttcca
    47341 tgccttccag ttgaccggat ttcaggcatt tagcatcgtg gctccattgc agatttttcg
    47401 ccacgtcttc ttttaaacca aactgcgcta ccagttccgg tgtcagacaa gcatagcttt
    47461 tgctcgcgtc atcttcacca gcataccagt aaatatcgta ctcttccgcc aatggctgtt
    47521 tatttagttg aacctgtaaa ttgtattttc cgggctcaac atatccctgg ctggaaaaac
    47581 gcttcagatc aatttttgtg tcgcctttta attccagaaa acgggaatca aactgaatgt
    47641 cgtcttcagc ccagacactg ctataactcc ctgacattgc cagcgcaatg caccacggaa
    47701 gaatccgtaa tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt ttttattctt
    47761 gttcggtcaa aatccattta aatcccccat agcagtgtcg ctatgggggt atgccttaca
    47821 ggtaagtaat ctggaaggtg gtatttgctt cgaaattacc cagatctggc gcatcagctg
    47881 cgccgaccag ccaggcttta aagttcagtg tctgcttcgc tttgccttta ttggtagaag
    47941 tgtctttcac tattttctgg tcaataccca tgccgctttt gtaagaggta cccagagagt
    48001 caccgatcgc caggctaaca ttgttaaatg ccgcaccggt atcggtattg aaaatggtgt
    48061 aataattgcc gctatttgca gaagaaacgg taccagtaaa ggtagtggtc attgtttcct
    48121 gagtatcaaa tacgcaatct tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt
    48181 tatttttcag tttttcggca ccgatatcac ccatatcaac ttccagtttg tgatcgtccg
    48241 gaacgataga acacactgag tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac
    48301 ccgcagcgat tgttgccgta gaggtgaaaa cgcccatcac cattgccgca gcaatagctg
    48361 ttttaacaaa cttactcatt atttgtcctt aaactataaa taattaaaaa tataaacatt
    48421 acaatacatt gattcagtca atacccaatg tttataccca gccattgaat acggcatgcg
    48481 atccaaaaag aacaggtcgt agaaaattgt
//
LOCUS       DAPPNJ010000005        47727 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000005 DAPPNJ010000000
VERSION     DAPPNJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.005"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            28..2271
                     /gene="fhuA"
                     /locus_tag="RPM73_000195"
     CDS             28..2271
                     /gene="fhuA"
                     /locus_tag="RPM73_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDX6518593.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            2322..3119
                     /gene="fhuC"
                     /locus_tag="RPM73_000196"
     CDS             2322..3119
                     /gene="fhuC"
                     /locus_tag="RPM73_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDX6518594.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3119..4009
                     /gene="fhuD"
                     /locus_tag="RPM73_000197"
     CDS             3119..4009
                     /gene="fhuD"
                     /locus_tag="RPM73_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDX6518595.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            4006..5988
                     /gene="fhuB"
                     /locus_tag="RPM73_000198"
     CDS             4006..5988
                     /gene="fhuB"
                     /locus_tag="RPM73_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDX6518596.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6023..7303)
                     /gene="hemL"
                     /locus_tag="RPM73_000199"
     CDS             complement(6023..7303)
                     /gene="hemL"
                     /locus_tag="RPM73_000199"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDX6518597.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7528..8949
                     /gene="clcA"
                     /locus_tag="RPM73_000200"
     CDS             7528..8949
                     /gene="clcA"
                     /locus_tag="RPM73_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDX6518598.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            8973..9038
                     /locus_tag="RPM73_000201"
     CDS             8973..9038
                     /locus_tag="RPM73_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518599.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9031..9375
                     /gene="erpA"
                     /locus_tag="RPM73_000202"
     CDS             9031..9375
                     /gene="erpA"
                     /locus_tag="RPM73_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDX6518600.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9422..10045)
                     /locus_tag="RPM73_000203"
     CDS             complement(9422..10045)
                     /locus_tag="RPM73_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDX6518601.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10083..10883)
                     /gene="btuF"
                     /locus_tag="RPM73_000204"
     CDS             complement(10083..10883)
                     /gene="btuF"
                     /locus_tag="RPM73_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDX6518602.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(10876..11574)
                     /gene="mtnN"
                     /locus_tag="RPM73_000205"
     CDS             complement(10876..11574)
                     /gene="mtnN"
                     /locus_tag="RPM73_000205"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDX6518603.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11658..13175
                     /gene="dgt"
                     /locus_tag="RPM73_000206"
     CDS             11658..13175
                     /gene="dgt"
                     /locus_tag="RPM73_000206"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDX6518604.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            13305..14729
                     /gene="degP"
                     /locus_tag="RPM73_000207"
     CDS             13305..14729
                     /gene="degP"
                     /locus_tag="RPM73_000207"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDX6518605.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            14884..16041
                     /gene="cdaR"
                     /locus_tag="RPM73_000208"
     CDS             14884..16041
                     /gene="cdaR"
                     /locus_tag="RPM73_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDX6518606.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(16130..16516)
                     /locus_tag="RPM73_000209"
     CDS             complement(16130..16516)
                     /locus_tag="RPM73_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDX6518607.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(16828..17652)
                     /gene="dapD"
                     /locus_tag="RPM73_000210"
     CDS             complement(16828..17652)
                     /gene="dapD"
                     /locus_tag="RPM73_000210"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDX6518608.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(17683..20355)
                     /gene="glnD"
                     /locus_tag="RPM73_000211"
     CDS             complement(17683..20355)
                     /gene="glnD"
                     /locus_tag="RPM73_000211"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDX6518609.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(20417..21211)
                     /gene="map"
                     /locus_tag="RPM73_000212"
     CDS             complement(20417..21211)
                     /gene="map"
                     /locus_tag="RPM73_000212"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDX6518610.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            21579..22304
                     /gene="rpsB"
                     /locus_tag="RPM73_000213"
     CDS             21579..22304
                     /gene="rpsB"
                     /locus_tag="RPM73_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDX6518611.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            22562..23413
                     /gene="tsf"
                     /locus_tag="RPM73_000214"
     CDS             22562..23413
                     /gene="tsf"
                     /locus_tag="RPM73_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDX6518612.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            23560..24285
                     /gene="pyrH"
                     /locus_tag="RPM73_000215"
     CDS             23560..24285
                     /gene="pyrH"
                     /locus_tag="RPM73_000215"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDX6518613.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            24577..25134
                     /gene="frr"
                     /locus_tag="RPM73_000216"
     CDS             24577..25134
                     /gene="frr"
                     /locus_tag="RPM73_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDX6518614.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            25226..26422
                     /gene="ispC"
                     /locus_tag="RPM73_000217"
     CDS             25226..26422
                     /gene="ispC"
                     /locus_tag="RPM73_000217"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDX6518615.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTT
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDLQEPQSVEDVLSVDATAREIARKE
                     VMRLAS"
     gene            26611..27369
                     /gene="ispU"
                     /locus_tag="RPM73_000218"
                     /gene_synonym="uppS"
     CDS             26611..27369
                     /gene="ispU"
                     /locus_tag="RPM73_000218"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDX6518616.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            27382..28239
                     /gene="cdsA"
                     /locus_tag="RPM73_000219"
     CDS             27382..28239
                     /gene="cdsA"
                     /locus_tag="RPM73_000219"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDX6518617.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            28251..29603
                     /gene="rseP"
                     /locus_tag="RPM73_000220"
     CDS             28251..29603
                     /gene="rseP"
                     /locus_tag="RPM73_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDX6518618.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            29633..32065
                     /gene="bamA"
                     /locus_tag="RPM73_000221"
     CDS             29633..32065
                     /gene="bamA"
                     /locus_tag="RPM73_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDX6518619.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            32187..32672
                     /gene="skp"
                     /locus_tag="RPM73_000222"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             32187..32672
                     /gene="skp"
                     /locus_tag="RPM73_000222"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDX6518620.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            32676..33701
                     /gene="lpxD"
                     /locus_tag="RPM73_000223"
     CDS             32676..33701
                     /gene="lpxD"
                     /locus_tag="RPM73_000223"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDX6518621.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            33806..34261
                     /gene="fabZ"
                     /locus_tag="RPM73_000224"
     CDS             33806..34261
                     /gene="fabZ"
                     /locus_tag="RPM73_000224"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDX6518622.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            34265..35053
                     /gene="lpxA"
                     /locus_tag="RPM73_000225"
     CDS             34265..35053
                     /gene="lpxA"
                     /locus_tag="RPM73_000225"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDX6518623.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            35053..36201
                     /gene="lpxB"
                     /locus_tag="RPM73_000226"
     CDS             35053..36201
                     /gene="lpxB"
                     /locus_tag="RPM73_000226"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDX6518624.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            36198..36794
                     /gene="rnhB"
                     /locus_tag="RPM73_000227"
     CDS             36198..36794
                     /gene="rnhB"
                     /locus_tag="RPM73_000227"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDX6518625.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            36831..40313
                     /gene="dnaE"
                     /locus_tag="RPM73_000228"
     CDS             36831..40313
                     /gene="dnaE"
                     /locus_tag="RPM73_000228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDX6518626.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            40326..41285
                     /gene="accA"
                     /locus_tag="RPM73_000229"
     CDS             40326..41285
                     /gene="accA"
                     /locus_tag="RPM73_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDX6518627.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            41384..43525
                     /gene="ldcC"
                     /locus_tag="RPM73_000230"
     CDS             41384..43525
                     /gene="ldcC"
                     /locus_tag="RPM73_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDX6518628.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            43582..43971
                     /locus_tag="RPM73_000231"
     CDS             43582..43971
                     /locus_tag="RPM73_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX6518629.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            44036..45334
                     /gene="tilS"
                     /locus_tag="RPM73_000232"
     CDS             44036..45334
                     /gene="tilS"
                     /locus_tag="RPM73_000232"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDX6518630.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(45383..45643)
                     /gene="rof"
                     /locus_tag="RPM73_000233"
     CDS             complement(45383..45643)
                     /gene="rof"
                     /locus_tag="RPM73_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDX6518631.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(45630..45830)
                     /locus_tag="RPM73_000234"
     CDS             complement(45630..45830)
                     /locus_tag="RPM73_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDX6518632.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            45996..46541
                     /locus_tag="RPM73_000235"
     CDS             45996..46541
                     /locus_tag="RPM73_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDX6518633.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            46538..46960
                     /gene="arfB"
                     /locus_tag="RPM73_000236"
     CDS             46538..46960
                     /gene="arfB"
                     /locus_tag="RPM73_000236"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDX6518634.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            46974..47684
                     /gene="nlpE"
                     /locus_tag="RPM73_000237"
                     /gene_synonym="cutF"
     CDS             46974..47684
                     /gene="nlpE"
                     /locus_tag="RPM73_000237"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDX6518635.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        11239 a        11738 c        13265 g        11485 t
ORIGIN      
        1 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
       61 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
      121 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
      181 gaaagcgcat gggggcctgc tgcaactatt gcggcgcgac agtctgctac cggcactaaa
      241 accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc cgaagagatg
      301 gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg tgtctctgtt
      361 ggtacgcgtg gcgcatccaa cacctatgac cacctgatca ttcgcggctt tgcggcagaa
      421 ggccaaagcc agaataacta tctgaatgga ctgaagttgc agggcaactt ctataacgat
      481 gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc ggtttccgtg
      541 ctttacggta aaagcagtcc tggcggcctg ttgaatatgg tcagcaagcg tccgaccacc
      601 gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca gactggtttt
      661 gactttagcg attcgttgga tgatgacggt gtttactctt atcgcctgac cggtcttgcg
      721 cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat tgcaccggcg
      781 ttcacctggc gtccggatga taaaaccaat tttaccttcc tttcttactt ccagaacgag
      841 ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc gctgccgaac
      901 ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta ttctcgtaat
      961 gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac tgtgcgtcag
     1021 aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta cggcgtctgc
     1081 tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc ggataaaggc
     1141 cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt ctccgttgat
     1201 acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct gaccggtgtc
     1261 gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga ctctgtgcca
     1321 ctgctcaatc tgtacaatcc ggtgaatacc gatttcgact tcaatgccaa agatccggca
     1381 aactccggcc cttaccgcat tctgaataag cagaaacaaa cgggcgttta tgttcaggat
     1441 caggcgcagt gggataaagt gctggtcacc ctgggcggtc gttatgactg ggcagatcaa
     1501 gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca gtttacctgg
     1561 cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag ctatagcgaa
     1621 tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc gtctaaaggt
     1681 aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt agttactggt
     1741 gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg ttccttcttc
     1801 tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa agcggcgctg
     1861 tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata caccaccgat
     1921 actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc gctgtgggct
     1981 gactatacct tctttgacgg tccgctttca ggtctgacgc tgggcaccgg tggtcgttat
     2041 actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta tacggtcgtg
     2101 gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa cgtggcgctg
     2161 catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac ttatggctgc
     2221 ttctggggcg cagaacgtca ggtcgttgca accgcaacct tccgtttcta atttctcttt
     2281 tggggcacgg attaccgtgc ccatttcaca agttggctgt tatgcaggaa tacacgaatc
     2341 attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt
     2401 tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatc ggtcacaacg
     2461 gttctggtaa atccactctg ctcaaaatgc ttggccgtca tcagcctccg acggaagggg
     2521 agattcttct tgatacccag ccgctggaaa gctggagcag caaagcgttc gcccgcaaag
     2581 tggcttattt gccgcagcag cttcctccgg cagaagggtt gaccgtgcgt gaactggtgg
     2641 cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggggcggca gatcgcgaaa
     2701 aagtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg ctggtcgata
     2761 gtctctctgg cggcgaacgt caacgggcgt ggatcgccat gctggtggcg caggatagcc
     2821 gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag gttgatgtgc
     2881 tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc gtgctgcacg
     2941 atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcggt ggtgaaatga
     3001 ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg atttatggca
     3061 tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt gtttattgat
     3121 gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc tttctccgtt
     3181 gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta ttgtggcgct
     3241 ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg gcgtggcgga
     3301 taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag tgattgacgt
     3361 cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt cgtttatggt
     3421 ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc cgggtcgcgg
     3481 atttaacttc agtgacggca aacagccgtt ggcgatggcg cgtaaatcgc tgacggaaat
     3541 ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat acgaagactt
     3601 tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac tgctgacgac
     3661 gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc aggaaattct
     3721 cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg gcagtaccgc
     3781 cgtcagtatc gatcgtctgg cggcgtataa agacgttgat gtgctctgtt ttgatcacga
     3841 caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca tgccgtttgt
     3901 ccgcgccgga cgctttcagc gcgtacccgc agtctggttt tatggtgcga cgctctcggc
     3961 aatgcacttt gtgcgcgttc tggataacgc catcggaggt aaagcgtgag taaacgaatt
     4021 gcgctttttc cggcgttatt gctggcgctg ctggtgattg tcgctacggc gctgacctgg
     4081 atgaactttt cgcaggcgct gccgcgcagt cagtgggcgc aggccgcctg gtcgccggat
     4141 attaacgtca tcgagcagat gatttttcac tacagcttgt taccacgtct ggcgatttct
     4201 ctactggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaac
     4261 ccgctggcgg aaccgacgac gttgggcgtt gctacaggcg cgcaactggg gattaccgtc
     4321 accacgctct gggcgatccc cggtgctatg gcgagccagt ttgccgcgct ggtgggcgct
     4381 tgtgtcgttg gcttaatcgt ctttggcgtc gcgtggggga aacggctttc gccggttacg
     4441 cttattctcg cggggctggt ggtgagcctt tattgcgggg caattaatca gctactggtt
     4501 atcttccatc acgaccagct gcaaagcatg ttcctgtgga gcaccggaac gctgacgcaa
     4561 accgactggg gcggcgttga gcgtttatgg ccgcagctgc tgggcggcgt gatgctgacg
     4621 ttactgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc gcgcaatctc
     4681 gggctggcct tatcgctcgc gcgtctggct gcgctgtcgc tggcgatagt catcagtgcg
     4741 ctgctggtga atgcggtggg gattatcggc tttatcgggt tgttcgcgcc actgctggca
     4801 aaaatgctgg gggcgcggcg tttgttgcca cggttgatgc tggcgtcgct gattggtgcg
     4861 ttaattctgt ggctttctga tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg
     4921 tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct gctgccgcgt
     4981 ttacggagca ttagcgcgcc ggatatgaag gtcaacgatc gtgtcgcgac tgaacgccaa
     5041 catgtgctgg cgtttgtcct cgcgggcggc gtgctgctgt tgatggctgt ggtagtggcg
     5101 ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt gcttgaggat
     5161 ttgatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt catgctggcg
     5221 gtggcgggct gtattattca gcggctgacc ggaaacccga tggcaagccc ggaagtgctg
     5281 gggattagct ctggcgcagc gtttggcgtg gtgttgatgc tgtttctggt gccgggtaat
     5341 gcctttggct ggctgttacc tgcaggcagt ctcggggcgg cggtgacgct gttgatcatt
     5401 atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc ggggatggcg
     5461 ttaagtaccg cgttcaccat gcttttgatg atgttgcagg caagtggtga cccgcgaatg
     5521 gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc gcaggtctgg
     5581 cgcaccggaa ttgtgatggt gattttgctg gcgattaccc cgctgtgccg ccgctggctg
     5641 accattttac cgctgggtgg tgataccgcc cgagccgtag gaatggcgct aacgccgacg
     5701 cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat gactattgga
     5761 ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg ctttcgacgg
     5821 acgatgccgc acatcgtaat ttcggcgctg gtgggtggtt tactgctggt gttcgctgac
     5881 tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct gtcaaccttt
     5941 atcggcgcgc catattttat ctatttgttg agaaagcaga gccgttaatt gaatgcctcg
     6001 cccggtgggc gaggcaaaat catcacaact tcgcaaacac ccgacgtgca gcatcgatgg
     6061 tgttattgat atcttccatg ctgtgcgcca cggacataaa gcccgcttca aacgctgatg
     6121 gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaag cgttccacgt
     6181 cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag aaaataccga
     6241 acatgccgcc aacgtggtta acgaccagcg gaattccggc ttcttctgcc gcttccagca
     6301 gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaacgccc ggctgcgcga
     6361 cttcattcag acaggcgaaa cccgctgcca tcgcaattgg gttaccggaa agcgtacccg
     6421 cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga ccaccgaatg
     6481 cgcctaccgg cattccaccg ccgatgattt tgcccaggca ggtgagatcc ggttccacac
     6541 cgtaataatc ctgtgcgcca gctagcgcta cgcggaagcc agtcattact tcatcgatga
     6601 tcagcaatgc gccaaattcg tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg
     6661 gtggaacgca gttcatattg cctgccaccg gctcgacgat aatacaggca atctcttgcg
     6721 ggtattgctc aaacgcggcg cgtacagaag ccagatcgtt ataagtacag gttaaggtat
     6781 gtttggcgaa atctgccgga acacccggcg agtttggctg gcctaacgtg agtgcgccag
     6841 aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca aatttaatga
     6901 ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc gcttcagtgc
     6961 cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc agttgcgcca
     7021 ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca
     7081 cggcattgcg gatagccgga tggttatggc ccagcaccat cggcccccag gaaccgacat
     7141 aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct ttttcgataa
     7201 acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga
     7261 tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt tcctgattcg
     7321 tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata ctcgtcaaac
     7381 atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt ttgtgcattt
     7441 gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta
     7501 tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt agaaacaccg
     7561 caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg cgataaaacc
     7621 ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct ggcagcggtt
     7681 gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct ggtacatact
     7741 gctgataatt atccgcttct gttaaccgtc gcttttctct gttcggcggt gctggcgatg
     7801 ttcggctact ttttggtgcg caaatacgcg ccggaagcag gtggttcggg gatcccggaa
     7861 attgaagggg cgctggaaga tcaacgtccc gttcgctggt ggcgtgtgtt gccggtgaaa
     7921 ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga aggaccaacc
     7981 gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct gaaaggtgat
     8041 gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac
     8101 gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt tcgctatacg
     8161 ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat gtaccggatt
     8221 tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg
     8281 ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat ttttaataaa
     8341 tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg
     8401 gtactgatgg gtggtgcgat tggcggtctg tgtggattgc tggggtttgt ggcaccagca
     8461 acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt cagcatggga
     8521 atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt ctcttccggc
     8581 gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg aaccgctttc
     8641 ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac gtttgctatt
     8701 gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg gatcattctg
     8761 gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac cggtcttggc
     8821 gcaacactat tagcgcaatt taccggcggg aaaccgctat actcggcgat tcttgcgcgc
     8881 acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc agccagcgag
     8941 aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg ttagaatttg
     9001 cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact gccgctggag
     9061 tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga taacccgaat
     9121 ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta tggtttcacc
     9181 tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt tggcctggtg
     9241 gttgatccga tgagcctgca atatctggtc ggcggttccg ttgattatac cgaaggtctg
     9301 gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct
     9361 tcctttagta tctaatctgt tgttcatgtg ccggataaac tatccggcac atcttctctc
     9421 ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa cgaatagccg
     9481 ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc agtggtacgg
     9541 aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca gttgcgtaga
     9601 tttctgtacg taaaatcatg gggatttcgc gggccagtac atcacgaatg atcccgccgc
     9661 caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc gcattaaagg
     9721 ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc accggcaaca
     9781 tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg ctggtgacca
     9841 ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga tccagcgcca
     9901 tgtcgcgaat tgtcccgccg cctacagcgg taaccacgcc cagtaccaga acaccaaaag
     9961 ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc gctgtgccga
    10021 ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga aaaggtcagg
    10081 cactaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat aatacgtggg
    10141 cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag ctgttcaccc
    10201 cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat cgcctgtggc
    10261 gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct gtctttaaag
    10321 atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt tccactggta
    10381 aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc agcatattgc
    10441 gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc cttgtctggt
    10501 tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca
    10561 tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg ctcggcatta
    10621 cctccacgcc aggcaatcac cagatcgggc ttcagcgcga caatgcgttc cagattcatc
    10681 ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg
    10741 ctgaccccaa ccggcgtgat cccggcagca aaggcaagtt cagtgttggc gggagaaagc
    10801 gtgatgacgc gcggcgcggc gttgagccac agtggcgcaa gaaaagacag ggcgaccagc
    10861 gccctgaaca gtgacttagc catgtgcaag tttctgcacc agtgactcaa ccatcaggct
    10921 ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact gttgatcggc
    10981 cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc agacatgggc
    11041 gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc ggattttcgc
    11101 cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt
    11161 aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt cagctttaaa
    11221 gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga catccgcgtc
    11281 gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcaacgttg gtgccaggcc
    11341 accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca gcagagtggc
    11401 acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct cggttccatt
    11461 cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt tttcgatttt
    11521 gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt tcatagattt
    11581 actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg aagcgtattt
    11641 ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac tggcatcgtc
    11701 gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc ttcgagagcg
    11761 atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt
    11821 ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc
    11881 aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt aaattgctgg
    11941 aagcatacgg cctggatgaa ctgaccggac cttttgaaag cattgttgag atgtcatgcc
    12001 tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg ataaatgact
    12061 ggtttcgcca gcgtttatat ccggaagatg ccgaaagcca gcctctgact gacgatcgct
    12121 gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag ctgcggcgca
    12181 agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt ctggtgcata
    12241 cattgatgag gatgaatctc acctgggcac aggttggcgg tattttaaaa tatacccgtc
    12301 cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa aagccgggtt
    12361 attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat ttggcgcttt
    12421 acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc tattgtgtgg
    12481 cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt tatcatcatt
    12541 tgcacgaggc gtggggtcag catgagaaag gttcgctctt ttcgctagtg gttgaaaatg
    12601 cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag ttttttatgt
    12661 atttacgggt aaacacccta aataaactgg taccatacgc ggcacaacga tttattgata
    12721 atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat gccagcgaat
    12781 gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt agccatccgg
    12841 atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta gagatttatc
    12901 gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa gaacgggtga
    12961 aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat cggctggcct
    13021 atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta tgggaatatt
    13081 attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc tatgcgtggg
    13141 atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa gacgaacaat
    13201 aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa cgaatctgaa
    13261 gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa aaaaccacat
    13321 tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc tctgcaacgg
    13381 cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca ccgatgctcg
    13441 aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc gttaatacgc
    13501 cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc tgccaggaag
    13561 gttcaccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat ggcggcggcc
    13621 agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat aaaggctatg
    13681 tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag ctgagcgatg
    13741 gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc gcgctgattc
    13801 agatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg
    13861 tgggtgatta caccgtagcg attggtaacc cgtttggcct gggagagacg gtaacttccg
    13921 ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa aacttcatcc
    13981 agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac ctgaacggcg
    14041 aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc ggtatcggtt
    14101 ttgctatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa tacggccagg
    14161 tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg gcgaaagcga
    14221 tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat tcctccgctg
    14281 caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag ccaatcagca
    14341 gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa ctgaccctgg
    14401 gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag agcagccaga
    14461 atcaggttga ttccagctcc atcttcaacg gcattgaagg tgctgagatg agcaacaaag
    14521 gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct gcgcagatcg
    14581 gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa aacatcgctg
    14641 aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt cagcgcggcg
    14701 acagctccat ctacctgtta atgcagtaat ctccctcaac cccttcctga aaacgggaag
    14761 gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat catcctttag
    14821 gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca taacggagga
    14881 cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc acgtaccatg
    14941 cgcatcatcg ataccaatat caacgtaatg gatgcccgtg ggcgaattat cggcagcggc
    15001 gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc acagggacga
    15061 gtcgtcgata tcgatgacgc ggtggcacgt catctgcacg gtgtgcggca agggattaat
    15121 ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag
    15181 aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct ggaacagtcg
    15241 cggttgatgc acttgttggc tcaggatagc cgtttgcggg aggaactggt gatgaacctg
    15301 attcaggcag aggagaatac tcccgcactt actgaatggg cgcaacggct ggggatcgat
    15361 ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct tggtgtggac
    15421 agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga gcgtaataat
    15481 ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt gaactctttt
    15541 gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac ccgcatgaaa
    15601 gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg tcctggcagt
    15661 attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca gcggatgcca
    15721 gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga cagtttgcgt
    15781 ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc gatggacaat
    15841 aacggcttgc tgcgacgaac gctggcggcg tggtttcgtc acaatgtgca accgctggca
    15901 acgtcaaagg cgttgtttat tcatcgtaat actctggagt atcggcttaa tcgtatatcg
    15961 gaactgaccg ggcttgattt gggtaatttt gatgacaggt tgctgctgta tgtggcgttg
    16021 cagctggatg aagagcggta ggttatgcgt taaggtcgag gcggcgctgg ctcattcccc
    16081 tcagcccgaa gggtagaggg gaatgtccga gttaaccgtc agttcggcat tatttacgcg
    16141 ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact gactccaggt
    16201 gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca cgctggcaga
    16261 tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg acttctttat
    16321 aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat ttaaacttaa
    16381 cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga atatcgttgt
    16441 tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata cccagacttt
    16501 tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct tattttagca
    16561 tttggcctgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt ccagacttct
    16621 atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc atgggatgga
    16681 ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta
    16741 tttaaaaaag gggggaagaa tgagtaggtc gaattattct gagccagaaa gcatacgggc
    16801 agcattatgc tgcccgtata tgtactctta atcgatggta cgcagcagtt cgttaatgcc
    16861 gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta
    16921 tttgccatct ttcgacggca gattacctga aacaaccaca gaccccgccg gaacgcgacc
    16981 gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc
    17041 catagagatg acggaacctt cttcgacaat caccccttca accacttcgg agcgtgcgcc
    17101 aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc
    17161 gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt
    17221 cgcccaggta tcaaccatcg tgccttcatc aacatatgcg ccgatgttga cgtaagacgg
    17281 catcagcacg gtgttacggg caataaatgc accctgacgt accgccgctg gtggcacaac
    17341 gcggaagcct tctttctgaa aacgcgcttc gtcgtagtcg gcgaatttca tcggcacttt
    17401 atcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag
    17461 cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac
    17521 acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc gggttacggt
    17581 gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg
    17641 taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg
    17701 attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg
    17761 ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg
    17821 ggcgccatga agcgaaattc ccagatcggc aaaaattttc ccgactctcg ccagcagtcc
    17881 tggttggtcg agcgcgataa gctcaaggaa cgatttgcgg tcggtatggg ttggcaaaaa
    17941 cgtcacttcg gtttcaacag taaagtgacg tagtttagcc ggttgacgac gcggctgtgg
    18001 tggctgccag ctactttgtg tcagtacctg ctccagacca aaccgaataa cctcatgacg
    18061 atctgcggat agcgggctac catcgggttc cagcacgatg aaggtatcca tcgccatacc
    18121 gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc
    18181 acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc
    18241 acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct gtaataaatg
    18301 gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg
    18361 tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc
    18421 ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg
    18481 tagctgtttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca
    18541 cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg
    18601 attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg
    18661 gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt
    18721 ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat
    18781 ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag
    18841 ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc
    18901 ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta
    18961 ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc
    19021 gccgagcacg ctatggcgat gcattggcaa tagcccgcgc cgcaccgctc cggggtgacg
    19081 cagaatgctc aaaaacagtt ttcgtgcttc cggaatatta cacagcggtt gttgcagatg
    19141 gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt
    19201 gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc
    19261 atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgattg gacgtggttt
    19321 ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag
    19381 ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt
    19441 gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacaggcg
    19501 attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag
    19561 acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccaa ccatttcatc
    19621 cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgga tatcgcgcaa
    19681 gccgccaggg ctacttttga tgtctggttc aagattgtag ctggtgccgt ggtaacgctg
    19741 atggcgctgg ttctgttctt caactttcgc cgcgtagaac ttgtcggaag gccagaatcc
    19801 ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg
    19861 ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccagca tgcactcttc
    19921 aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc
    19981 gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat
    20041 gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcgacca atgccaggtc
    20101 ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt cgataaactc
    20161 ggtgcgcgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca
    20221 acgctggaaa gtatcgatat gggcttttat cccaccgacg gttaattcat cacggggcca
    20281 gacgcatgga ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg cgtactgttc
    20341 tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc
    20401 attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc
    20461 gtagcgtcag aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag
    20521 acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt
    20581 tacccgcgtt gaccattggc tcgatggtga acgtcatccc aggtttcagt acgacgttgg
    20641 tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt
    20701 gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaatcgccg
    20761 caccgatttc gcgcagatta atgcctggtt tgaccatgcg cagcgccagg tacaggcttt
    20821 cttgcgtgat gcggcacaga cgttcgccca tgatggtcgg cttaccgacg ataaacattt
    20881 tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc
    20941 catctttcag cagcttagca tcgtccggga taccgtggca caccacttca ttaatagaga
    21001 tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat
    21061 taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat
    21121 acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga
    21181 tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc
    21241 gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct
    21301 gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac atctgatgct
    21361 gtacacagcg ccaacaatta ttggtgtcca tgacgtattt gtggtataaa gcgcgccgga
    21421 cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca
    21481 acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt
    21541 ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg
    21601 acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga
    21661 agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac
    21721 cgatgttcaa cgaagctctg gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc
    21781 ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg
    21841 accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc
    21901 gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaaa
    21961 agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc
    22021 tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc
    22081 acgaacacat tgctatcaaa gaagcaaaca acctgggtat tccggtattt gctatcgttg
    22141 ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc
    22201 gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg
    22261 atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc
    22321 ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat
    22381 cagtaagtga ccgggatgag cgagcgaaga caacgcatct gcggcgcgaa atatgaaggg
    22441 ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    22501 ttttcacttt tatatctgtg cggtttaatg ccgggcagat cacatctccg aggattttag
    22561 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    22621 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    22681 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    22741 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    22801 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    22861 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    22921 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    22981 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    23041 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    23101 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    23161 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    23221 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    23281 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgcttcga
    23341 agtgggtgaa ggcatcgaga aagttgagac tgactttgcg gcagaagttg ctgcgatgtc
    23401 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    23461 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    23521 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    23581 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    23641 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    23701 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    23761 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    23821 ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct
    23881 attccattga atggcgtgtg cgacagctac agctgggcag aagctatcag cctgttgcgc
    23941 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    24001 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    24061 gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg caaccatgta cgagcaactg
    24121 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    24181 gctcgtgacc ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc
    24241 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    24301 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    24361 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    24421 ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    24481 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    24541 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    24601 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    24661 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    24721 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    24781 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc
    24841 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    24901 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    24961 cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    25021 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    25081 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    25141 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    25201 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    25261 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    25321 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    25381 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    25441 gggtagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    25501 ggatgttgat caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc
    25561 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    25621 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    25681 acataacgcc atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    25741 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    25801 gacgccattg cgcgatttgg caacaatgac gccggatcaa gcctgccgtc atccgaactg
    25861 gtcgatgggg cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata
    25921 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    25981 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    26041 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    26101 tggtgtgaag ccgctcgatt tttgcaaact gagcgcgttg acatttgccg caccggatta
    26161 tgatcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggctgcaac
    26221 gaccacattg aatgccgcaa acgagatcac tgttgcagct tttcttgcgc aacagattcg
    26281 ctttacggat attgctgcgc tgaatttatc tgtactggaa aagatggatt tacaagagcc
    26341 gcaaagcgtg gaagatgtgc tgagcgtaga tgctactgcg cgtgaaatcg ccagaaaaga
    26401 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgtgct atttgttagc
    26461 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttattaggtc
    26521 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    26581 taaaccctgt cagggaataa gaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    26641 aaattgccag cgcatggctg tcgtcatgtt gcgatcataa tggacggcaa tggtcgctgg
    26701 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    26761 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagc
    26821 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    26881 gcgcttgata gcgaagtaaa aagcctgcac cgacataacg tgcgtctgcg tattattggc
    26941 gataccagtc gctttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    27001 gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    27061 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    27121 atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    27181 gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat
    27241 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    27301 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    27361 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    27421 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat cgtaacgctg
    27481 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    27541 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    27601 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg
    27661 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    27721 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    27781 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    27841 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    27901 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    27961 ataggtggac tagcgaccgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    28021 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    28081 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    28141 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    28201 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    28261 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    28321 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    28381 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    28441 tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    28501 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    28561 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    28621 tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa ttcgatagct gcggaagcac
    28681 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    28741 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    28801 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    28861 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    28921 ctgtactgga aaatgtgcaa ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    28981 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    29041 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcaggga agtcccttgt
    29101 ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    29161 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    29221 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    29281 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    29341 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    29401 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    29461 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    29521 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    29581 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    29641 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    29701 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    29761 cctcagtatg ccggtgcgca caggcgacac ggttaatgat gaagatatca gtaataccat
    29821 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    29881 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    29941 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    30001 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    30061 cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    30121 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    30181 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    30241 gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct
    30301 tgaaaccctg cgcagctact atctggatcg cggttatgcc cgtttcaaca tcgactctac
    30361 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg
    30421 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    30481 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa
    30541 gatggaagat gacatcaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    30601 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc
    30661 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    30721 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctgggaagcg atctggtcga
    30781 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    30841 acgtgttccg ggtagcccgg accaggtaga tgtcgtctac aaggtaaaag agcgcaacac
    30901 cggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    30961 tgtgcagcag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    31021 cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcactg tagatggtgt
    31081 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    31141 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa
    31201 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    31261 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    31321 tagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    31381 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    31441 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    31501 cgatcacaaa tgggttgttc tggggcgtac ccgctggggt tatggtgatg gtttaggcgg
    31561 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    31621 ccagtccaat acaattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    31681 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    31741 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    31801 gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac
    31861 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    31921 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    31981 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    32041 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    32101 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    32161 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtctcggtt
    32221 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaac atgggcagcc
    32281 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    32341 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc
    32401 agtccatgaa agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc
    32461 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    32521 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    32581 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    32641 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    32701 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    32761 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    32821 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    32881 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    32941 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    33001 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    33061 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    33121 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    33181 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    33241 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    33301 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactattatt ggcaatggcg
    33361 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    33421 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    33481 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    33541 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    33601 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    33661 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    33721 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    33781 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    33841 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    33901 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    33961 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    34021 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg aactgtacta
    34081 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    34141 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    34201 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    34261 ataagtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    34321 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    34381 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    34441 taatgagatt tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg
    34501 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    34561 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    34621 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    34681 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    34741 ccatcagttc tgcatcattg gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca
    34801 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    34861 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    34921 taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    34981 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    35041 tctgattcgt taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    35101 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    35161 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    35221 atggaagaac tggcggtaat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    35281 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    35341 attgatgcgc ccgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    35401 accattcatt acgtcagtcc atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    35461 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    35521 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    35581 gataaaaatg ccgcccgtga tgtgctgggg atccctcacg atgcccactg cctggcgttg
    35641 ctaccgggga gccgtggcgc agaagttgaa atgcttagtg ccgatttcct gaaaacggcc
    35701 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccactggt gaatgccaaa
    35761 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctttc agttcatttg
    35821 ctggatggga tgggccgtga ggcgatggtc gccagcgatg cggcgctact ggcgtcgggt
    35881 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    35941 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    36001 ctgctggcgg gcagagagtt agtcaaagaa ttattgcagg aagagtgtga gccgcaaaaa
    36061 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat
    36121 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    36181 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    36241 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    36301 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    36361 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    36421 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    36481 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    36541 taccgatgcc tgcgatggct gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg
    36601 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    36661 cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc ttttcatctg gaaaaactgg
    36721 ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg
    36781 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    36841 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
    36901 ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    36961 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    37021 ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag ttaacccacc
    37081 tgacggtact ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag
    37141 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    37201 taaacgaagg gttgatcctt ctttccggcg gacgcatggg cgacgtcgga cgcagtcttt
    37261 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    37321 atcgctattt tctcgagctg atccgcaccg gcaggccgga cgaagaaagc tatctgcacg
    37381 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgaccaac gacgtgcgct
    37441 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    37501 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    37561 aggagatgtg tgagctgttt gccgacatcc ccgaagccct tgccaacacc gttgagatcg
    37621 ccaaacgctg taacgtaacc gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    37681 gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
    37741 tggccttttt attccctgat gaggaagaac gtcttaagcg ccgcccggaa tatgacgaac
    37801 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    37861 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    37921 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    37981 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    38041 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtggcggac atgtacggtc
    38101 gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg
    38161 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc
    38221 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    38281 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    38341 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    38401 attttgcgcc gctttactgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    38461 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    38521 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
    38581 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    38641 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    38701 aacctgactg cttcgaagat atgatcgcac tggtggcgct gttccgccca ggtccgttgc
    38761 aatcagggat ggtggataac tttatcgacc gtaaacatgg tcgcgaagaa atctcctatc
    38821 cggacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca
    38881 tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat accctcggtg
    38941 gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    39001 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    39061 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    39121 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    39181 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    39241 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    39301 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    39361 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    39421 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    39481 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    39541 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    39601 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    39661 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac ctgaccggac
    39721 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    39781 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    39841 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    39901 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    39961 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    40021 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    40081 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    40141 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    40201 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    40261 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    40321 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    40381 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    40441 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatcttg
    40501 gtgcatggca gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg
    40561 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata
    40621 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    40681 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    40741 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg ccaatcatca
    40801 cctttatcga caccccgggg gcttatccgg gcgtgggcgc agaagaacgt ggtcagtctg
    40861 aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg
    40921 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    40981 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    41041 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    41101 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggtggcgct caccgtaacc
    41161 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    41221 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    41281 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    41341 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    41401 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    41461 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    41521 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    41581 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    41641 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    41701 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccgacg aatatcttga
    41761 taacattaca ccgccgttca cgaaagcctt gtttacctac gttaaagagc ggaagtacac
    41821 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct
    41881 gttttatgat tttttcggcg ggaataccct caaggctgat gtctctattt cggtcaccga
    41941 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg
    42001 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    42061 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca attgtcataa
    42121 atcgctggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    42181 taatgcgttg gggattcttg gtgggatccc gcgccgtgaa tttactcgcg acagcatcga
    42241 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga ttaccaactc
    42301 cacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    42361 gatccacttc gattccgcct gggtgccgta cacccatttt catccgatct accaggggaa
    42421 aagtggtatg agcggcgagc gtgttgcggg aaaagtgatc ttcgaaacgc aatcgaccca
    42481 caaaatgctg gcggcgttat cgcaggcttc gctgatccac attaaaggcg agtatgacga
    42541 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    42601 tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa
    42661 ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc cagcggttgc gggaagagtc
    42721 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgctggcc
    42781 cgttgcgcca ggcgaacagt ggcacggctt taacgatgcg gatgccgatc atatgtttct
    42841 cgatccggtt aaagtcacta ttttgacacc ggggatggac gaacagggca atatgagcga
    42901 ggaggggatc ccggcggcgc tggtggcaaa attcctcgac gaacgtggga tcgtagtaga
    42961 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggtatcgata aaaccaaagc
    43021 aatgggatta ttgcgtgggt tgacggaatt taaacgctct tacgatctca acctgcggat
    43081 caaaaatatg ctacccgatc tctatgctga agatcccgat ttctaccgca atatgcgtat
    43141 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttc ccggtttgat
    43201 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    43261 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    43321 aaatatgatc ctgccttatc caccgggcgt accgctgttg atgccaggag aaatgctgac
    43381 caaagagagc cgcacggtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    43441 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    43501 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgtgctg
    43561 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    43621 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    43681 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    43741 tatgtgattg agcttttctc ttttccgttc ccgccggagc gccccagtcg accggaagct
    43801 tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt
    43861 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    43921 accttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    43981 tcgccgcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    44041 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    44101 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt
    44161 cactctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    44221 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    44281 tgcgcaagag gggctgggga ttgaggccca agcgcggcag gcgcgttatc aggcatttgc
    44341 ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    44401 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggggga
    44461 agtctcggag tttgccggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    44521 actggcgcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    44581 cgactcatac gatcgtaact ttctgcgcct gcgtgtagtg ccgttattgc agcagcgttg
    44641 gccgcatttt gccgaagcaa cggcccgcag cgccgcactt tgtgctgaac aagagagtct
    44701 gttggatgaa ctgctggcgg atgatttagc acactgccaa acgtcgcagg ggacgctgca
    44761 gattgcgcca atgctagcga tgagtgatgc ccgtcgcgcg gcgattatcc gccgctggct
    44821 ggcagggcag aatgcaccga tgccttcccg cgacgcgctg gtgcggatct ggcaggaagt
    44881 ggcgctggcg cgggaagatg cctccccctg tttacgcttg ggcgcgtttg aaatccgccg
    44941 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagtgaaa ccattgtgcc
    45001 gtggcagacg tggctacaac cgctggaatt accggcgggg ctgggaagcg tacagcttac
    45061 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    45121 ggcaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    45181 agagttaggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggcgaaac
    45241 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    45301 cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa gccggataag acgcatcaaa
    45361 cgtcgcatcc ggcgaaagtc aatcaggact cgctcaccac caccgtaccg atttccgggt
    45421 ggctaaagct ggtaatttta tccagacgta gctcacgggt ttcgccagcg gcctcgacga
    45481 ccagatactc cacatttttg cgggagacta aatcgctggc ttttgcctgc aatttttcgc
    45541 catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca agattatcgt
    45601 aatcatcaca attgattggt tgatacgtat cattcattga cataatcgct caccagtaag
    45661 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    45721 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    45781 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    45841 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    45901 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    45961 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    46021 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    46081 tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcttgctg gcgtggctga
    46141 aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    46201 cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg ctgccggatg
    46261 agcggcggat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt acctataata
    46321 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc
    46381 tttccgtctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    46441 ccatgacgct gcaggcaacg attcaggatg gcgtgatctg gttatcggac gataagaata
    46501 atctggaagt taacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    46561 tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg cgggcgggca
    46621 gcatgttaat aagacctcaa cggccattca tctgcgtttt gacattcggg cgtccagcct
    46681 gccagagtat tacaaagagc gtctactcgc cgccagccat catttgatca gcagtgatgg
    46741 cgtgattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagccgcgct
    46801 agcccggctg gtggcagtga ttaaagattt aacaacagaa caaaaagccc gacgacccac
    46861 gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    46921 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tagatggtga
    46981 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    47041 accgggccga agtcgatacg ctttctccag cacaggctgc cgaactgaaa ccgatgccgc
    47101 aaagttggcg cggcgtgctg ccgtgtgctg attgcgaagg aatcgaaacc tctctgttcc
    47161 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
    47221 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
    47281 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
    47341 aaggcaatcc gattgaatcg cagttcaact atacgctgga accggcacaa tctagtttac
    47401 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
    47461 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
    47521 acctggctgc gcgcggtcac agtgaaaaac cggtgttact gtcagtagaa ggtcacttta
    47581 cgcttgaggc taatccggat actggtgcgc caaccaaagt attagcgccc gatacggcgg
    47641 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
    47701 aacaaacgca aaattgcctg atgcgct
//
LOCUS       DAPPNJ010000006        47482 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000006 DAPPNJ010000000
VERSION     DAPPNJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.006"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            53..685
                     /gene="comR"
                     /locus_tag="RPM73_000238"
     CDS             53..685
                     /gene="comR"
                     /locus_tag="RPM73_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDX6518636.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(747..1286)
                     /locus_tag="RPM73_000239"
     CDS             complement(747..1286)
                     /locus_tag="RPM73_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDX6518637.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="RPM73_000240"
     CDS             complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="RPM73_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDX6518638.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3217..3759)
                     /locus_tag="RPM73_000241"
     CDS             complement(3217..3759)
                     /locus_tag="RPM73_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX6518639.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQS
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3782..4807)
                     /gene="nagZ"
                     /locus_tag="RPM73_000242"
     CDS             complement(3782..4807)
                     /gene="nagZ"
                     /locus_tag="RPM73_000242"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDX6518640.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4818..5642)
                     /gene="thiK"
                     /locus_tag="RPM73_000243"
     CDS             complement(4818..5642)
                     /gene="thiK"
                     /locus_tag="RPM73_000243"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDX6518641.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5623..6264)
                     /gene="lpoB"
                     /locus_tag="RPM73_000244"
     CDS             complement(5623..6264)
                     /gene="lpoB"
                     /locus_tag="RPM73_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDX6518642.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6278..6655)
                     /locus_tag="RPM73_000245"
     CDS             complement(6278..6655)
                     /locus_tag="RPM73_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDX6518643.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="RPM73_000246"
     CDS             complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="RPM73_000246"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDX6518644.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7351..9540
                     /gene="fhuE"
                     /locus_tag="RPM73_000247"
                     /pseudo
     CDS             7351..9540
                     /gene="fhuE"
                     /locus_tag="RPM73_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9600..11033)
                     /gene="ptsG"
                     /locus_tag="RPM73_000248"
     CDS             complement(9600..11033)
                     /gene="ptsG"
                     /locus_tag="RPM73_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDX6518645.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11328..12125)
                     /locus_tag="RPM73_000249"
     CDS             complement(11328..12125)
                     /locus_tag="RPM73_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX6518646.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12136..13140)
                     /gene="holB"
                     /locus_tag="RPM73_000250"
     CDS             complement(12136..13140)
                     /gene="holB"
                     /locus_tag="RPM73_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDX6518647.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13137..13778)
                     /locus_tag="RPM73_000251"
     CDS             complement(13137..13778)
                     /locus_tag="RPM73_000251"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDX6518648.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13768..14790)
                     /gene="yceG"
                     /locus_tag="RPM73_000252"
     CDS             complement(13768..14790)
                     /gene="yceG"
                     /locus_tag="RPM73_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDX6518649.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14793..15602)
                     /gene="pabC"
                     /locus_tag="RPM73_000253"
     CDS             complement(14793..15602)
                     /gene="pabC"
                     /locus_tag="RPM73_000253"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDX6518650.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLHNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15722..16963)
                     /gene="fabF"
                     /locus_tag="RPM73_000254"
     CDS             complement(15722..16963)
                     /gene="fabF"
                     /locus_tag="RPM73_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDX6518651.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17051..17287)
                     /gene="acpP"
                     /locus_tag="RPM73_000255"
     CDS             complement(17051..17287)
                     /gene="acpP"
                     /locus_tag="RPM73_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX6518652.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17498..18232)
                     /gene="fabG"
                     /locus_tag="RPM73_000256"
     CDS             complement(17498..18232)
                     /gene="fabG"
                     /locus_tag="RPM73_000256"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDX6518653.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18245..19174)
                     /gene="fabD"
                     /locus_tag="RPM73_000257"
     CDS             complement(18245..19174)
                     /gene="fabD"
                     /locus_tag="RPM73_000257"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDX6518654.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19190..20143)
                     /gene="fabH"
                     /locus_tag="RPM73_000258"
     CDS             complement(19190..20143)
                     /gene="fabH"
                     /locus_tag="RPM73_000258"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDX6518655.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20211..21281)
                     /gene="plsX"
                     /locus_tag="RPM73_000259"
     CDS             complement(20211..21281)
                     /gene="plsX"
                     /locus_tag="RPM73_000259"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDX6518656.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21362..21535)
                     /gene="rpmF"
                     /locus_tag="RPM73_000260"
     CDS             complement(21362..21535)
                     /gene="rpmF"
                     /locus_tag="RPM73_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDX6518657.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21587..22108)
                     /gene="yceD"
                     /locus_tag="RPM73_000261"
     CDS             complement(21587..22108)
                     /gene="yceD"
                     /locus_tag="RPM73_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDX6518658.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22307..22891
                     /gene="yceF"
                     /locus_tag="RPM73_000262"
     CDS             22307..22891
                     /gene="yceF"
                     /locus_tag="RPM73_000262"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDX6518659.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23003..23962)
                     /gene="rluC"
                     /locus_tag="RPM73_000263"
     CDS             complement(23003..23962)
                     /gene="rluC"
                     /locus_tag="RPM73_000263"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDX6518660.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24535..27720
                     /gene="rne"
                     /locus_tag="RPM73_000264"
     CDS             24535..27720
                     /gene="rne"
                     /locus_tag="RPM73_000264"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDX6518661.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPSPKAAPTTPAAPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNSRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQTEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAIEPVVSAPVIEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVTQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="RPM73_000265"
     CDS             complement(27917..28870)
                     /gene="flgL"
                     /locus_tag="RPM73_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDX6518662.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDLLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="RPM73_000266"
     CDS             complement(28882..30525)
                     /gene="flgK"
                     /locus_tag="RPM73_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDX6518663.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWHVTRLASNTTFSVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="RPM73_000267"
     CDS             complement(30591..31532)
                     /gene="flgJ"
                     /locus_tag="RPM73_000267"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDX6518664.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYTAVTIAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="RPM73_000268"
     CDS             complement(31532..32629)
                     /gene="flgI"
                     /locus_tag="RPM73_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDX6518665.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="RPM73_000269"
     CDS             complement(32641..33339)
                     /gene="flgH"
                     /locus_tag="RPM73_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDX6518666.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="RPM73_000270"
     CDS             complement(33392..34174)
                     /gene="flgG"
                     /locus_tag="RPM73_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDX6518667.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="RPM73_000271"
     CDS             complement(34312..35067)
                     /gene="flgF"
                     /locus_tag="RPM73_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDX6518668.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="RPM73_000272"
     CDS             complement(35087..36292)
                     /gene="flgE"
                     /locus_tag="RPM73_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDX6518669.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="RPM73_000273"
     CDS             complement(36317..37012)
                     /gene="flgD"
                     /locus_tag="RPM73_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDX6518670.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="RPM73_000274"
     CDS             complement(37024..37428)
                     /gene="flgC"
                     /locus_tag="RPM73_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDX6518671.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="RPM73_000275"
     CDS             complement(37432..37848)
                     /gene="flgB"
                     /locus_tag="RPM73_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDX6518672.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38003..38662
                     /gene="flgA"
                     /locus_tag="RPM73_000276"
     CDS             38003..38662
                     /gene="flgA"
                     /locus_tag="RPM73_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDX6518673.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38738..39031
                     /gene="flgM"
                     /locus_tag="RPM73_000277"
     CDS             38738..39031
                     /gene="flgM"
                     /locus_tag="RPM73_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDX6518674.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39036..39452
                     /gene="flgN"
                     /locus_tag="RPM73_000278"
     CDS             39036..39452
                     /gene="flgN"
                     /locus_tag="RPM73_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDX6518675.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="RPM73_000279"
     CDS             complement(39492..41027)
                     /gene="murJ"
                     /locus_tag="RPM73_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDX6518676.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41137..42060)
                     /locus_tag="RPM73_000280"
     CDS             complement(41137..42060)
                     /locus_tag="RPM73_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX6518677.1"
                     /translation="MKKLPIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42062..42709)
                     /locus_tag="RPM73_000281"
     CDS             complement(42062..42709)
                     /locus_tag="RPM73_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDX6518678.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="RPM73_000282"
     CDS             complement(42720..43304)
                     /gene="rimJ"
                     /locus_tag="RPM73_000282"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDX6518679.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43540..44748
                     /gene="mdtH"
                     /locus_tag="RPM73_000283"
     CDS             43540..44748
                     /gene="mdtH"
                     /locus_tag="RPM73_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDX6518680.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGCSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44812..45459
                     /gene="grxB"
                     /locus_tag="RPM73_000284"
     CDS             44812..45459
                     /gene="grxB"
                     /locus_tag="RPM73_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDX6518681.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45593..46153
                     /locus_tag="RPM73_000285"
     CDS             45593..46153
                     /locus_tag="RPM73_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6518682.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46259..47305
                     /gene="pyrC"
                     /locus_tag="RPM73_000286"
     CDS             46259..47305
                     /gene="pyrC"
                     /locus_tag="RPM73_000286"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDX6518683.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47379..>47482
                     /locus_tag="RPM73_000287"
     CDS             47379..>47482
                     /locus_tag="RPM73_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDX6518684.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFP"
BASE COUNT        11479 a        12865 c        11801 g        11337 t
ORIGIN      
        1 tgattatttt tataataacc tttcattcac agtcaccagg tacaacggca tcatggcaac
       61 tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc
      121 cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc
      181 ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa
      241 agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga
      301 agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc
      361 catcgccaac tgctttacaa gcaaagacac cccggcaggc tgcttcatga tcaacaactg
      421 caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat
      481 gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac
      541 ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat
      601 cagcgcacgc gaaggtgcat cgctggaaaa actaatgcag attgccggaa cgactttgcg
      661 tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag
      721 gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccatta
      781 ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg
      841 ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta
      901 caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta
      961 ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc acgaccacca
     1021 ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga
     1081 ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt
     1141 gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccccgt
     1201 tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac accaatcccg
     1261 atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt atgccaatca
     1321 tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga
     1381 aatacttaag gattcggagg agtctgaagg aatgcctgat gcgacgctta acgcgtctta
     1441 tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc
     1501 agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac
     1561 cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag
     1621 cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt
     1681 acccatcagg ctaccgacgg tggagaagtt cgacagcgat accagcgaac catgatcttt
     1741 atactgataa tttttcagcg gcttaccgtt catctgcgcc agtatgttgt tcattgcgca
     1801 agtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg
     1861 gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcgg
     1921 ttccaccacc agctggttga tacggttagt ttcaagacca ccgatatctt tcaggaagtc
     1981 tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt cgccatcttt
     2041 agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc
     2101 aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg caggcaaaat
     2161 acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc
     2221 gtagctgtgc agttgcttga ccgcgttgtg caattcagcg gagagttcta cacccgtcgc
     2281 gccgccgccg acaatcgcaa tgttcacttt gccatttgcg cccaggttgg cggagtattt
     2341 caggaagaga ttcagcatct cctggtgaaa gcgacgcgcc tggtgcgggt tatcgaggaa
     2401 aatgcagttc tctttgacac ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat
     2461 taccagggtg tcataggcga ttttacgctc cggaaccagc agttcacctt tctcatcgcg
     2521 cagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg aacccagctg
     2581 gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc
     2641 aagcgagcca gtcgccactt cgtgcagcag cggtttccac aggtggctgt ggttacgatc
     2701 gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat
     2761 ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg tagtcaacgt
     2821 gaccccctta aatttattaa ccaattgtta attaatggtt attaacatag ctcttaatta
     2881 acaacaggtt acaacaatga aaatgttctt cgctattgag aataacatga atggtgcatt
     2941 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
     3001 ggtgagatca aaaaacagag tttattgttt attatttaag cgaatctgtg agagtgatat
     3061 agagtgaggg gtgtatgcag ggcgttcggt aggcctgata agaggcgcat caggcattga
     3121 tgctcgactg tcggatgcgg cgtaaacgcc ttatccgacc cacagaatcc ggagcgcatc
     3181 aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt
     3241 aatgcgctgc aaatgcgggg agatattctt gaatttgtgc gactgctctt cgtcccagac
     3301 aatctcgtaa taatgatgca gctcttcaga tgtccgctgg ctgttaagcg cttcatcatt
     3361 acgcgacaaa atcaccaggc aacgatcgcg attcttctca cggaagttgg tcacacactt
     3421 agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg
     3481 gaacaaatta gggttgaaga tcacctggcg gatgtcgcaa agaaaaccaa tccgttccgc
     3541 ccagtatccg cccaggccaa cgccgcaaat tagcggacgc tcgtcaacgt tcagttgcaa
     3601 cattttgtcc acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta
     3661 gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc
     3721 cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg
     3781 gttagtgacc tgctttctct tcctgccagc gttcgtgtaa ctgattcaga cgggtgctga
     3841 tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca
     3901 aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt
     3961 tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatgcc tgcccgcgtt
     4021 cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc
     4081 cgtcaaaacc cagttcctga cgcaaaacgg ttttcagcca gtagggagaa ccgctcgccg
     4141 gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt
     4201 cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct tgtggacgtg
     4261 gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt
     4321 tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg gcaattgcca
     4381 gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg atatgcccga
     4441 catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca
     4501 gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcctgacagc gcagcgaatg
     4561 attgcgccgc tggcaagcgg gtaaaacctt cacgaaaacg ctgcacgcgt ccaccttcct
     4621 gatctaccgc caccaccaga tgattgcgcg aagctgcgcg gatctggcgc accagttcac
     4681 gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat
     4741 gcgcaagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg
     4801 gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc
     4861 ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc
     4921 atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc
     4981 gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacact
     5041 gccgccagtt ccagcgcgat atcaccatct ccggcatact cccagtcgat gagttttaac
     5101 cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt
     5161 aaaggccgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc
     5221 cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc
     5281 cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc
     5341 ggcaaatacg tttttacctc gccgggcaga tagtcgacta ccatccagtc acggagatat
     5401 aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata ctggcgcaag
     5461 aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga
     5521 tgttcaatga gaaaactccc gccactaagc ccactattaa acgtcgtgac gggatgaaac
     5581 tgcgggaaaa agcgcgacag caattcgtcg cgcatgatgg gattattgct gcgaaacggc
     5641 acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt
     5701 cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg
     5761 ggcaatgcct atggctttac tacgggtacc taaactgtcc tgcggcgaca aacctaactg
     5821 ttgcttagcc atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag
     5881 cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt
     5941 aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact
     6001 gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc
     6061 cgtttgatct tcgtgctcaa ttgggcctgg ttgctgcggg atcgtcggca ccgagggcac
     6121 tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac
     6181 cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa
     6241 ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga
     6301 ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa
     6361 tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt
     6421 aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg
     6481 aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata
     6541 aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac
     6601 agcccaccag cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta
     6661 cagaccttta tgtgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat
     6721 gtggtaaacc tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc
     6781 ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg
     6841 ctcatgctca gctgacacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat
     6901 atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac
     6961 gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac
     7021 attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta
     7081 tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa
     7141 aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa
     7201 aagacattct ctgtttggtt tacacgacaa atattaatgc gtatatttct catttgcatt
     7261 tacaaacaaa attattcgca cactaaatat aatcgctcat caccctcctc cggcatgagc
     7321 ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat
     7381 caatatcaag ccatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta
     7441 ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc
     7501 acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa
     7561 atgcagatga ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg
     7621 gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa
     7681 agtcaggcgg attccgatcg tgctctttat tattcccgcg gattccagat cgataactat
     7741 atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct
     7801 gatatggcac tctttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg
     7861 acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgaccag tcgtgaattt
     7921 aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta
     7981 caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat
     8041 aacgactcat ggctggaccg ctacaacagt gaaaagacct tcttctcggg cattgtcgat
     8101 gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt
     8161 aatagcccta cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac
     8221 gatcgtgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc
     8281 tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac
     8341 tctgaagtcg aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat
     8401 ggtatgctgg ttgggccata cagtaattat ggacctggct ttgattatgt cggcggcacc
     8461 ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtatttat
     8521 taattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat
     8581 cgttacttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt
     8641 aatggcaatt tcccacaaac cgactggtca ccacagagcc tggcacagga cgataccaca
     8701 catatgaaat cgttatatgc tgccactcga gtcacccttg ccgatccgct gcatctgatc
     8761 ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac
     8821 cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc
     8881 agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct
     8941 ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg
     9001 accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca
     9061 cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa
     9121 ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca
     9181 acgcgctata ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc
     9241 acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt
     9301 ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc
     9361 gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac
     9421 ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac caacgtggaa
     9481 ggttctatcg tctacggcac accgcgtaat ttcagcatta ccggcacgta tcaattctga
     9541 ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacggat
     9601 tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa
     9661 atcgcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca
     9721 gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg
     9781 aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg
     9841 gtacctgtcg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt
     9901 gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc
     9961 gggaacagcc acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc
    10021 gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc
    10081 agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc
    10141 tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc
    10201 gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac
    10261 attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc
    10321 ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg
    10381 aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg
    10441 taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt
    10501 gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg
    10561 ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta
    10621 atacggtaga aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt
    10681 acgccagtat ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt
    10741 ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg
    10801 gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg
    10861 tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag
    10921 ctgaaattcg cggaaccgac gcccagcagg atacctgcga taggcagtac ggataccggc
    10981 agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag
    11041 agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct
    11101 cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg
    11161 ccaatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt
    11221 gacaaaacat atgtcacaat atttcaaacg tggttattca ccgcccaatt ttcggcggtg
    11281 aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat
    11341 tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc
    11401 agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc
    11461 gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc
    11521 accagtaatc gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg
    11581 aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt
    11641 tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc
    11701 agaattgcca gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt
    11761 tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt
    11821 gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga
    11881 cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa
    11941 aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt
    12001 gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc
    12061 acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa
    12121 aacatgatgt ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg
    12181 ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg taacagacat
    12241 taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag
    12301 atggtttgcc agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcacc
    12361 atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc
    12421 ttgttcatga ttaagggccg ctagcagcga ataccagtcg cccgatggca cgctatatgc
    12481 caacgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc
    12541 cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt
    12601 cacttcgcgt gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa
    12661 ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa
    12721 ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt
    12781 cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt
    12841 cagcttttcg gtgacctcac gtaccgcatc aatgcccagc atatttttcc ctttttcggg
    12901 agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg
    12961 accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat
    13021 taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc
    13081 ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat
    13141 gcgtccaact ccttaaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc
    13201 agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag
    13261 cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg
    13321 cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg
    13381 gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca
    13441 cgtcctccgc cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg
    13501 ccgttagcca gagctggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac
    13561 atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagtacc
    13621 aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg
    13681 cggataccca gttgctcaag cgtctcaacc accacattac gcgcggtagt tttgcctgcg
    13741 ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag
    13801 ataatcctgc acagacttgt tatggctggc aagattggta ttaaacgtgt gaccaccttt
    13861 accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga
    13921 atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata
    13981 cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc
    14041 gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac
    14101 cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat
    14161 cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct
    14221 atcgaccgct ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt
    14281 ggcggtatac atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag
    14341 ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg
    14401 agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg
    14461 gaactgtgct tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg
    14521 tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca
    14581 ttgaaaaacc cgtgggcgat tgatgatctt atcagcataa agctgttcac cgagcgccag
    14641 acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc
    14701 ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa
    14761 taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct
    14821 aaatattcat ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc
    14881 actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag
    14941 gcttgcactt cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc
    15001 ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc
    15061 cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc
    15121 tcatcagcgt ttgtctgctc aagatgagag cggatcaata cttgctcaag ccgattaaga
    15181 tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc
    15241 atcccttcgt tatgcaaacg gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc
    15301 gttgccggtc cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg
    15361 actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga
    15421 ggccagaagt cacagaaaat catcaaccgc tgacaagcat cctgtagtcg ctggatatgc
    15481 gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca
    15541 aactgcgttg cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac
    15601 ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg
    15661 gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg aaaatgacaa
    15721 cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca
    15781 gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac
    15841 cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga
    15901 tggagtagat agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg
    15961 tggagcttac caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt
    16021 cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg
    16081 cttcaatgcc tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt
    16141 ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt
    16201 aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac
    16261 catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt
    16321 tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac
    16381 tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg
    16441 cagcatgacc aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc
    16501 cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa
    16561 cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga
    16621 tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt
    16681 ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa
    16741 tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct
    16801 ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga
    16861 tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag
    16921 acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag
    16981 ggaaaaaatg attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg
    17041 agatgttcac ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga
    17101 ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca
    17161 gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa
    17221 cttcttcctg cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag
    17281 tgctcatact cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata
    17341 aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt
    17401 gaggccttac cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca
    17461 gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt
    17521 tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg
    17581 cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg
    17641 cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa
    17701 cgtttacagt aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca
    17761 agcccgcttt cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag
    17821 aaccgatagt gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg
    17881 acagacggaa aacagatgaa aggttggttt cgataatatc gttccactct tcatctttca
    17941 ttcgcattaa caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac
    18001 caaattctgc gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca
    18061 acatcagacc tttgccgtta gcacctaaat aatcactgat cgcctgagcg ccattttcac
    18121 tggtcgcagt gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc
    18181 caattccgcg gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc
    18241 tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca
    18301 gggtgtcgac aatgcgtttc gtcaggccag ttagcacttt gcccgggccg acttcataga
    18361 gatgttctac gccttgcgct gccatgtact caacagactt cgtccactga accgggttat
    18421 acaactgacg taccagtgcg tcacgaatgg catcaccatt ggtttcgcat ttcacatcaa
    18481 cgttattcac aacaggaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca
    18541 gtttgtcggc tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca
    18601 gcgcgcgttt tgcgcccgca gctttacagg cagcgccagc acgctcaacc gcctctttat
    18661 gaccggcaat aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt
    18721 ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca
    18781 tagcgcccgt gccttctggt acggcttctt gcatgaactt gccgcgcatc tcaaccagac
    18841 gcaccgcatc agcgaaatca atcacgccag cgcaaaccag cgcggagtat tcccccaggc
    18901 tgtgaccggc catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg
    18961 caacagatgc agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg
    19021 gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg
    19081 tttcttcgac aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac
    19141 cctgtccagg gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa
    19201 ccagcgcgga gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg
    19261 gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg
    19321 tattaccgtg gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg
    19381 ccgttgcact gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac
    19441 ggtcaagatt atttgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa
    19501 ccttgaagac ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacacggt
    19561 cagcattagg cagcgtcagc aactcaccat agctaccgtc ggcatgcaga tgggtggaga
    19621 tgattcccgg ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa
    19681 taatagtccc acgatcggtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca
    19741 gagcatactt caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga
    19801 aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc agcatgcttt
    19861 gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca
    19921 ggccaatctg gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc
    19981 ccatggttga aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag
    20041 tgacaatcca ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt
    20101 gttcgggcag atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt
    20161 tcggttatat accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga
    20221 gttccgcttt tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga
    20281 gcggcaattc gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc
    20341 gcaaaagctc gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac
    20401 agacaggcgc cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt
    20461 tgtagccaac gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat
    20521 ttcagcagag aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct
    20581 gtaaagccgt cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga
    20641 tagccgatat aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg
    20701 tcgagaccct ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt
    20761 tccaccacct cttcagccag gactgagccc ataatggcaa attgcaccaa cattgtgctg
    20821 tcacaatcga cgttggcccc taaatcgaga accaccgttt tgcccttttg ctgatgcggc
    20881 aataccgtca ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc
    20941 agccccatca gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc
    21001 accaactcca gggccacgcg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc
    21061 cggacatcac tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca
    21121 aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg
    21181 agttgcgaat tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa
    21241 tcccctccca tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt
    21301 ttggtctttc cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg
    21361 attacttagc gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca
    21421 ggtgtttttc accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat
    21481 gggaacgacg catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc
    21541 ttactcctca attacggttt atccccgatc caacggggag caccaattac ttacgcttta
    21601 agctggctaa tacggcaaat gggtttggct tttgcgcttc ttcaggcagt tcaccaaaga
    21661 ccatgtccgc ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg
    21721 cgaggatgat ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct
    21781 caatcggttc atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa
    21841 aacaatacgt tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg
    21901 ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg
    21961 acatggagca ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct
    22021 gatcaggggt atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga
    22081 gagtcagggg taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag
    22141 agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt
    22201 tactgcttta ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata
    22261 gtttaaaatg gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat
    22321 tttagcctcc acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga
    22381 atgtgcagca ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt
    22441 gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat
    22501 tattggttca gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga
    22561 agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg
    22621 actggcgttg tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt
    22681 ccatttccgt cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct
    22741 gcactgcgcg ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga
    22801 gggacgtgac cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg
    22861 tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca
    22921 tccggcaatc ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca
    22981 ttatcaggct tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca
    23041 aaccttcatc catcggcgct tcgatgcgca tcacctcacc ggtccccgga tgggtaaact
    23101 tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc
    23161 tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg
    23221 tatgcacacg gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa
    23281 atgcatagcg ctcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac
    23341 gcacgatacg ttcgccgctt tgcagaatat ttttcagtaa cggcgcttga acgctcttca
    23401 catgcgactg ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac
    23461 gtaattgctc atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg
    23521 aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc
    23581 gcaaaccttc gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag
    23641 gtttattcag caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca
    23701 ccttttgcag atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggaa
    23761 tacgcacctc atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc
    23821 gcacttcgcc tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg
    23881 tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta
    23941 cggatggagt ctctgttttc atggttggcg attctaatta gccaacagga ttcgcgccac
    24001 tcatttttct atgcttatat ttacttttgc accttattac ttcactgcgt gatcacttta
    24061 ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat
    24121 aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg
    24181 tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat
    24241 ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc
    24301 agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata
    24361 attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga
    24421 cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag
    24481 gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa
    24541 agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag
    24601 cgtctgtatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac
    24661 aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct
    24721 gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac
    24781 agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt
    24841 cagatcgata aagaagagcg cggaaacaaa ggcgcggcat taaccacctt tatcagtctg
    24901 gcgggtagct atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt
    24961 atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa
    25021 ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc gctgcaatgg
    25081 gatttaagct tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg
    25141 gctccgttcc tgattcatca ggagagcaac gtaatcgttc gcgcattccg cgattactta
    25201 cgtcaggaca tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag
    25261 catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag
    25321 atcccgctgt tcagccacta ccagatcgag tcgcagatcg aatccgcctt ccagcgtgaa
    25381 gtccgtctgc catccggtgg ttccattgtt atcgacagca ccgaagcgtt aactgccatc
    25441 gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacacc
    25501 aacctcgaag ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg
    25561 attgttatcg acttcatcga catgacgcca gtacgccacc agcgtgcggt agaaaaccgt
    25621 ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt
    25681 ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac tgggtgaatc cagtcatcac
    25741 gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct
    25801 attctgcgtc tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt
    25861 gttcctgtgc caatcgcttc ttacctgctg aatgaaaaac gttctgcggt gaatgccatt
    25921 gaaacgcgtc aggacggtgt tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg
    25981 cactaccacg tgctgcgcgt gcgtaaaggg gaagaaaccc caaccttaag ctacatgctg
    26041 ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag
    26101 cgtccggaac aacctgcgct ggcaaccttt gccatgccgg atgtgccgcc agcgccaacg
    26161 ccagctgaac ctgccgcgcc tgtcgtagct ccatcaccga aagctgcacc gacaacacca
    26221 gcagctcctg cacaacctgg gctgttgagc cgcttcttcg gcgcactgaa agcgctgttc
    26281 agcggtggtg aagaaaccaa accgaccgag caaccagcac cgaaagcaga agcgaaaccg
    26341 gaacgtcaac aggatcgtcg caagcctcgt cagaacagcc gccgtgaccg taatgagcgc
    26401 cgcgacaccc gtagcgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat
    26461 cgtcgccagg cacagcagca gactgccgag acgcgtgaga gccgtcagca gactgaggta
    26521 acggaaaaag cgcgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc
    26581 cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa
    26641 tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag
    26701 cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca
    26761 ccggtggttg aagaaactgt cgctgccgaa ccaattgttc aggaagcgcc agctccacgc
    26821 acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa
    26881 gagaacaatg ccgataaccg tgacaacggt ggtatgccgc gtcgttctcg ccgctcgcct
    26941 cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc
    27001 cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc
    27061 tggatccgct atccaattgt acgtccgcaa gatgtacagg ttgaagagca gcgcgaacag
    27121 gaagaagtac aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tatcgaaccg
    27181 gttgttagcg cgccagttat tgaagaagtg gctgaagtcg tagaagcccc cgttcaggtt
    27241 gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtaattgc cgccgcggta
    27301 actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga agttgcagaa
    27361 cacgcagaac cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa
    27421 actgctgagg ttgtggttgc tgaacctgaa gttgttactc aacctgccgc gccagtcgtc
    27481 gctgaagtcg cagcagaagt tgaaacggta gccgcggttg aacctgagat caccgttgag
    27541 cataaccacg ctaccgcgcc aatgacacgc gctccggcac cggaatatgt tccggaggca
    27601 ccacgtcaca gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt
    27661 ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa
    27721 taattagctc aaagtaatca agccctggta actgccaggg cttttttatt tcatctttga
    27781 atcaacatat taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa
    27841 aaaaaatgcc cgccccggcg atcccagagc aggcagacaa aacataccca gtttcatgat
    27901 atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa
    27961 atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat
    28021 tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaatgcg cgatcgctac
    28081 ctaataaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggtca
    28141 gcacattgtt cagcgagttt ttcagtccgc ggttggtttt atctaacgcc gcagcggcgg
    28201 tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca
    28261 gcatggcaaa aagatttgtt tcagaagcgc taccgtctgg ttccgctacc gcgttgctgg
    28321 taatactgtc gaaaattttg tcacccgtgt gccctatcac catcgaacgc gaagcatcga
    28381 cctgttgttt aatactttct gctccaccga cgtatttccc tttctcttcg ctaaacggcg
    28441 cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca
    28501 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt
    28561 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg
    28621 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgcgcca
    28681 gcgtgtactg gctgttttgc gcctgcgcct gggagagaac aaccgcttgt gatgcagcaa
    28741 tgggatcatc tgaagggtta acgactcgct tacccgtcga catctgttcg ccgtacttca
    28801 tccattctgc ctgagaattg gtaatgccgc gcatgttttg ctggtacatc atctgggtac
    28861 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    28921 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    28981 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    29041 attaccttgc gtggtgctac tggttttcaa cgtagcggtt ttattaccga tatcactcac
    29101 taacgaggca taagcgtcat taaaagattt cgcaccgccc accgttttac tgttgctttg
    29161 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    29221 catcgcgatt ttcgcttcgt cagtgattaa tacatccatg ttgacgatgg cgtcacttac
    29281 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    29341 atcaaatgcc actttaccgt tggcatccgg cgtcaccgaa aaagtggtat tgctggcaag
    29401 gcgggtgaca tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc
    29461 ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag
    29521 aaccgcgggc ttaccgatag caaagaaatc ttcaccagca tcgccgttgg catcaaatcc
    29581 ggctttgtgt tgggtgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt
    29641 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    29701 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacataag cgacagtcgt
    29761 acgagaaggg tcggcgctgg aaggaactgc cgcaagttgc cgcgccgtac ttccctgaac
    29821 cagtgagtaa ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa cgctgacttc
    29881 tacgccaaca atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt
    29941 aggtgacgcc cctgccccca cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat
    30001 ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt
    30061 gtcctggtcg cgcagatatt gatcggtggt tttaaactga ttcaccaacc cttctgattt
    30121 cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct
    30181 ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc
    30241 gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgcagcacg
    30301 taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt agacgccatt
    30361 gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg
    30421 ggtatatcca gcaacgttat agctggagat attattactt gccgtattta acgccgcctg
    30481 ggccgcgttc agtccgctca tggcgttatt aatcaagctg gacatggagg ttccttataa
    30541 gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga
    30601 ttatcaatgt tcatactgta ggttttgctc accttgtcgc ttatcgattt catctgctga
    30661 atcatgttgg tgagtttgcg ggcatagtga ggatcggtgg catagcccgc gtcctgtagg
    30721 gcctgcgccc cctgttccgc actcgcggcg atcgtcacgg cggtgtagcg cgggttacgc
    30781 gttaacagcc caacgtaatc cgacaaggct tccagatacg agctgtagac gcgaaacttc
    30841 gcttttactt tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc
    30901 cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg
    30961 cggcggattt gccgttgccc ccaaccagat tccagtgccg cctgagcgag gatcaaatga
    31021 tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat
    31081 gctttactgt cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc
    31141 tgcgaaagcg cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct
    31201 gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct
    31261 gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa tttgctggtc atacatactg
    31321 gtatacagac gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg
    31381 cttttcaaca tcatctgcac gaacatccct tccacctggc gggccaccgg acggatattt
    31441 gccgccggat cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc
    31501 gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac
    31561 atcccgcact ttgcattgat tgcaaaatag acatcagatc catcggcgta gcgcccagcg
    31621 cattgagcgc gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac
    31681 cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa
    31741 acggtgtatc tggctggctg acattggcct gacgattgac tgttactgag agatttccct
    31801 gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg
    31861 agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatctg
    31921 caaggaaacg gacctgggaa ctgttgccac ttggtacgcg cacctgaata gtccgcgcat
    31981 ctaacgcggt ggcgctgcca tatccacgca cgcgattgat ggtgtcagcg atttgctgcg
    32041 ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccaacg ccaaactggc
    32101 tgggcaattc acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttca
    32161 cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg
    32221 ccagcgcata cacctgactg tcaacgccct taagcggcgt catcaacaac gtacctccac
    32281 gcaagctttt ggcatttccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc
    32341 caaacggagg aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg
    32401 tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa
    32461 acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa
    32521 tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag
    32581 ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac
    32641 ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt
    32701 aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt
    32761 attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc
    32821 ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg
    32881 gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc
    32941 gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg
    33001 caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc
    33061 agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat
    33121 attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga
    33181 ctggaaaata gaaccgttgg cgacgggcgt cggcccggga accggttgtg cactggtcgc
    33241 cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa
    33301 gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca
    33361 gtaaaccggt gaacctgcca ccggttaagc cttagagttg cgtcagtttt tgcagcatct
    33421 gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat
    33481 tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc
    33541 ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc agaggattgc gtttcggtgt
    33601 agaggttttc gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct
    33661 gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac
    33721 caatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac
    33781 cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg
    33841 atgaaccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc
    33901 tgttgttggt ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg accgggcgta
    33961 cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg
    34021 gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt
    34081 tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa
    34141 ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac
    34201 aagcagagca gaagcacgcg acagtgtgaa tttcccctca ccctaaccct ctccccaaag
    34261 gggcgaggga atagtccgcg cgcttttatt gcaatgtcat caatcgttca attaactcat
    34321 cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg
    34381 catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt
    34441 actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca gtatcggccc
    34501 acgcgtggcc tggctttctg cgcttaaacg aaaaatgccg tcgtcaccgc gttgcacttc
    34561 gctgcccgtg gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc
    34621 gcccggattg agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg
    34681 cacagcaatt ggcccagcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt
    34741 gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggactgcac
    34801 ggccagccag ccatcctgct gcaatgcaac gtccagcggg cgcgaggtgt aatccatctt
    34861 gccgggcgtc atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg taggcagtga
    34921 aagcccttcc accggcactg cacgcaaggc gtttaactgt gcgcgaaaac cgggcgttga
    34981 ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc
    35041 catcgcggta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    35101 ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    35161 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg
    35221 caccgttggt cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag
    35281 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    35341 agttcgccag tacaatctgc cccagcagtt gtgtttgttc gttggaatag ttgccgacaa
    35401 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg
    35461 tggttgccac aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc
    35521 taaacgtggc gggatctgcg ccgttaattg cgccagtggt aatattctct gttggatttg
    35581 aggtcagaac gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg
    35641 gatcactgct atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga
    35701 cgctcatgtc atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat
    35761 agctatccgc attgctggcg ctgaatggcg taacagtagg aagcggatcg ctggaattca
    35821 ggttgatctg catcgatgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg
    35881 aaatattggt cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac
    35941 ccgtcagctg taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc
    36001 cgttacggct gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc
    36061 tgatagcaac gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag
    36121 tgataccggc aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag
    36181 aggccgtgcc tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat
    36241 cgaggttggt ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact
    36301 cctgaaatgt gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag
    36361 tgccgagatc cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact
    36421 gcagcggctg ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag
    36481 aaccgttcgg cgcagtgctg ccatcagtca acgtaccgtc ccaggtgaaa ctgtgaactc
    36541 cggcggtcag ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg
    36601 tggccgtaac tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca
    36661 cagccccttc ttcactgccg gttcccgcaa gaacagttgt gccggggatc atcacgccgt
    36721 gaccgatcag gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc
    36781 cgagcgtggt attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg
    36841 tcagctcgtt gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca
    36901 aagtcagaaa actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg
    36961 tggtactgac gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct
    37021 cctttattga ccgagcgtaa gggttttcag catcatactt ttcaccgtgt tgagcacttc
    37081 aacgttggcc tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc
    37141 aacgttcggc atttttacgt agccctttgc atctgccagc ggattacccg gttcataaac
    37201 cagtttgtcc ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc
    37261 acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg
    37321 accggtcacg ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg
    37381 ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc
    37441 gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg
    37501 gtattgcagg ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc
    37561 gtcaagcgaa ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc
    37621 ctgcgccggg atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg
    37681 tccacgttgc atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc
    37741 aggggtatcg gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt
    37801 gagcgcctct tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca
    37861 ggtatcaaaa ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa
    37921 cgcaaaatgg gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt
    37981 gtagaaatgg ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat
    38041 actgttcagt ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag
    38101 cgcccaactc gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct
    38161 actaccgcca tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa
    38221 tgtgaatgtg ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc
    38281 cacaggaaat tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg
    38341 caatgtcaaa ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat
    38401 caatcaactt gttgatgcca ttagcctgcg cgatctatca cccgatcaac ctatccagtt
    38461 aacccagttt cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag
    38521 cggtgatggg tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca
    38581 gaatgcgcgg gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg
    38641 gaatattctt ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc
    38701 aacacatgat aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc
    38761 tctgaagcct gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag
    38821 ccgggcggca aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc
    38881 aaaactgatg caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact
    38941 ggcgattcgt aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa
    39001 cgaagcgcag caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg
    39061 accagatgtc cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc
    39121 tctctatggg gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac
    39181 tgctggcgac gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata
    39241 gcgttgatat tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa
    39301 tgaatcaaca taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg
    39361 aaatgttgaa accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa
    39421 cccatcgcgg cggtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc
    39481 ggaatgcatt gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc
    39541 agtgcagcga agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc
    39601 cagggcatgg tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca
    39661 gacatcacca gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcgga
    39721 gtaaagattt tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc
    39781 agaccaatag aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc
    39841 aattgcgtta aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg
    39901 gaataaaagc caggagccaa aactttcact acaatcaggc caatcaaacc caccgagtag
    39961 gcaattaacg cccgctgggt catcagcgca tcaaaggcgg taaatttacc gtactggaac
    40021 agcgaaacgg tcaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac
    40081 aggaaacaaa gacgcagccc ccagtccatc aaacggttgt attcatcatg attgccactg
    40141 gcaaaactct tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacacca
    40201 gacggaaact ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga
    40261 aacgaggcaa aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc
    40321 gccggtccca tctgtttcac cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc
    40381 aggaccagca tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggatgccg
    40441 ccgaccgtaa cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac
    40501 agcgcgaaac caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag
    40561 cggttccacg tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag
    40621 ggaaaggtaa tctttagtag ctggctggtc agggcaaatt tgtcggcagt gtcagcgaag
    40681 cctggcgcgg tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc
    40741 gccagcgcga gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca
    40801 ccctgcttac ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg
    40861 gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc
    40921 atccctgcgc caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa
    40981 aacatggtca tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc
    41041 cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg
    41101 attatggtga attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca
    41161 gatcttgtca acgatacgtt gcgccagcac ggcctgttcg ccggcggttt gcggaactgt
    41221 ctgattttgc acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgttcaag
    41281 agtgctctgc caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca
    41341 ttcgcgcata tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg
    41401 ctgacttccg gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg
    41461 gtgctcggca aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga
    41521 ggctttgccg cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa
    41581 cgtgaaataa agatcgtgtg gcccgacact attgctacga tgtttatcca ttcttagcga
    41641 agctgcggta gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa
    41701 accgaccatc aacgtcagtt ttttacgcgc cgccagttcc accagccgtt cagcatcgcg
    41761 cagattttct gccagcggtt tatcgacaca gacatgtaca cccgcattga gtaacgtact
    41821 gaccacgtca aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc
    41881 aaggctggat aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt
    41941 cgcgcgcgta ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg
    42001 taaccacgct ttttgcgcaa tgccacctaa tcccactacg ccgataggta attttttcac
    42061 tttaatctcc cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga
    42121 tttccagggc ttcgacgcgg gcctgtaaat caccatcaac cgcgttcgac atatccgtca
    42181 ccgccggctg atcttcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac
    42241 ccggttcgcg ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca
    42301 gcgtcgactc cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca
    42361 gttcacctgg cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttccgctg
    42421 cgctcagttt cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc
    42481 gattaccaaa accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt
    42541 cctgcacttc ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg
    42601 ccgtgactac accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca
    42661 gcaagcaacc aatcacgcga gcttcaaggg cagtaagttg atatttcatg agattctcct
    42721 tagcggccgg gcgtccagtc tggggtcgtt aatgccgtca gtacgtgatc gcgccattgt
    42781 ccatcaatca acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt
    42841 aaatcaccgc tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg
    42901 gtgcgctgca tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc
    42961 catttttgcc caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg
    43021 gaaaaattgg caacgccaat aatctctttt tcgtccgggt cgaataaacc aaagtagaaa
    43081 gctgaacctt gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga
    43141 taacagtggc tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg
    43201 taataatccg caagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct
    43261 gtggttaagc gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt
    43321 cccgtaacgt ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa
    43381 aagtccaaaa tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc
    43441 gacgttttga taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca
    43501 gaatagaaag tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg
    43561 aggaacctgg gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt
    43621 gttgtcttcc cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg
    43681 gtcggtattg ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc
    43741 gcaattgccg accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgct
    43801 gccggattcg ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg
    43861 ctctcgggac tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta
    43921 atccgtccac aacagcgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt
    43981 gcagtcattg gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc
    44041 gccacagggg ccgttttgtt tgtgctgtgt gcagcgttca atgcgtggtt gttaccagca
    44101 tggaaactct ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac
    44161 aagcgttttg tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg
    44221 atgctgatgc tgccaattat ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg
    44281 atgtatgcca ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt
    44341 gaaaagcatt ttcgtctgga acaccgattg atggctgggc tgttgataat gtcattaagc
    44401 atgatgccgg tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt
    44461 tatatcgggt cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac
    44521 gcaagagctc gcggcagcta tatggggtgt agccgtctgg gtctggcgat tggcggcgct
    44581 attggttata tcggtggcgg ctggctgttt gacctgggta aatcggcgca ccagccagag
    44641 cttccgtgga tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag
    44701 tttagccaga aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc
    44761 ccctgactcc tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta
    44821 tacatttacg atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat
    44881 atccccgtcg aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc
    44941 ggtcaaaaac aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg
    45001 gacatcgttc actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc
    45061 cctgccattg aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg
    45121 cgttttgcca aatcggcatt tgatgagttt tctactcccg ccgcacgcaa atatttcgtc
    45181 gacaagaaag aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg
    45241 attaagaata tcagcgatga tttacgtgcg ctggataaac tgatcgtcaa accgaacgcc
    45301 gtgaatggcg aactttcaga agatgatatt cagctatttc cgctactacg taatctgacg
    45361 ctggtagccg gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa
    45421 cagacacaaa tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc
    45481 tgtattcgcg ggttgccgcc gttttcagtt ttttctgctg gtgagtttat cgctggtgac
    45541 acatgctgcg ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa
    45601 gtttttattt gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac
    45661 ccaatacacc atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc
    45721 aaaagatatc ggtttacccg gcgtggctga cgcccatatt gttctgacaa acctgaccag
    45781 ccaaattggt cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat
    45841 gaactccctg ttcggtagcc agaaagcgac catgaaactg aagctgaaag cgctgcccgt
    45901 gtttgataaa gaaaaaggcg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt
    45961 acaaccggaa aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt
    46021 acgcaattac tttaaccagc aacctgctta cgtcctgcgc gaagatggca gccagggcga
    46081 agcaatggcg aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc
    46141 atttactgat taatcacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg
    46201 tttccgctta tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat
    46261 gactgcacca tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg
    46321 cgatggcgac atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat
    46381 cgtaatgccc aatctggctc cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca
    46441 acgtattctt gatgccgtac ctgccgggca caatttcacc ccattgatga cctgttattt
    46501 aacagattcg ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc
    46561 tgcaaaactt tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga
    46621 cgcaatcatg ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg
    46681 tgaagtgaca catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt
    46741 gatggaacct ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac
    46801 caaagatgct gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc
    46861 gcagcatctg atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta
    46921 ttgtttaccc atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag
    46981 cggttttaat cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga
    47041 gagcagttgc ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac
    47101 cgtctttgaa gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc
    47161 gcagttctat ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca
    47221 ggttgctgaa agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac
    47281 ggtacgctgg tccgttaaac aataaaaatt gtagcttcct gttgtcatta ggttatttta
    47341 cctgtataaa taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag
    47401 cgaaaacttc tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc
    47461 gtattcagta tgcgtttcct ga
//
LOCUS       DAPPNJ010000007        44377 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000007 DAPPNJ010000000
VERSION     DAPPNJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.007"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(79..1443)
                     /gene="mnmE"
                     /locus_tag="RPM73_000288"
     CDS             complement(79..1443)
                     /gene="mnmE"
                     /locus_tag="RPM73_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDX6518685.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1549..3195)
                     /gene="yidC"
                     /locus_tag="RPM73_000289"
     CDS             complement(1549..3195)
                     /gene="yidC"
                     /locus_tag="RPM73_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDX6518686.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3198..3455)
                     /gene="yidD"
                     /locus_tag="RPM73_000290"
     CDS             complement(3198..3455)
                     /gene="yidD"
                     /locus_tag="RPM73_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDX6518687.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3419..3778)
                     /gene="rnpA"
                     /locus_tag="RPM73_000291"
     CDS             complement(3419..3778)
                     /gene="rnpA"
                     /locus_tag="RPM73_000291"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDX6518688.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3795..3935)
                     /gene="rpmH"
                     /locus_tag="RPM73_000292"
     CDS             complement(3795..3935)
                     /gene="rpmH"
                     /locus_tag="RPM73_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDX6518689.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4165..4245)
                     /locus_tag="RPM73_000293"
     CDS             complement(4165..4245)
                     /locus_tag="RPM73_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518690.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4542..5945
                     /gene="dnaA"
                     /locus_tag="RPM73_000294"
     CDS             4542..5945
                     /gene="dnaA"
                     /locus_tag="RPM73_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDX6518691.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5950..7050
                     /gene="dnaN"
                     /locus_tag="RPM73_000295"
     CDS             5950..7050
                     /gene="dnaN"
                     /locus_tag="RPM73_000295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDX6518692.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7050..8123
                     /gene="recF"
                     /locus_tag="RPM73_000296"
     CDS             7050..8123
                     /gene="recF"
                     /locus_tag="RPM73_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDX6518693.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8152..10566
                     /gene="gyrB"
                     /locus_tag="RPM73_000297"
     CDS             8152..10566
                     /gene="gyrB"
                     /locus_tag="RPM73_000297"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDX6518694.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10806..11204
                     /locus_tag="RPM73_000298"
     CDS             10806..11204
                     /locus_tag="RPM73_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDX6518695.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11319..12131
                     /gene="yidA"
                     /locus_tag="RPM73_000299"
     CDS             11319..12131
                     /gene="yidA"
                     /locus_tag="RPM73_000299"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDX6518696.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12177..12833)
                     /locus_tag="RPM73_000300"
     CDS             complement(12177..12833)
                     /locus_tag="RPM73_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518697.1"
                     /translation="MKLNLKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13111..13800
                     /gene="dgoR"
                     /locus_tag="RPM73_000301"
     CDS             13111..13800
                     /gene="dgoR"
                     /locus_tag="RPM73_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDX6518698.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13797..14675
                     /gene="dgoK"
                     /locus_tag="RPM73_000302"
     CDS             13797..14675
                     /gene="dgoK"
                     /locus_tag="RPM73_000302"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDX6518699.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14659..15276
                     /gene="dgoA"
                     /locus_tag="RPM73_000303"
     CDS             14659..15276
                     /gene="dgoA"
                     /locus_tag="RPM73_000303"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDX6518700.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15273..16421
                     /gene="dgoD"
                     /locus_tag="RPM73_000304"
     CDS             15273..16421
                     /gene="dgoD"
                     /locus_tag="RPM73_000304"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDX6518701.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16496..17833
                     /locus_tag="RPM73_000305"
     CDS             16496..17833
                     /locus_tag="RPM73_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6518702.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17830..18888)
                     /gene="cbrA"
                     /locus_tag="RPM73_000306"
     CDS             complement(17830..18888)
                     /gene="cbrA"
                     /locus_tag="RPM73_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDX6518703.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYINRHAFD
                     LWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSMVR
                     RHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAYPM
                     KDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAGFI
                     SASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTAPA
                     LRKWIMRSGMAHIPQLKD"
     gene            18989..20203
                     /locus_tag="RPM73_000307"
     CDS             18989..20203
                     /locus_tag="RPM73_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDX6518704.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20205..20537)
                     /locus_tag="RPM73_000308"
     CDS             complement(20205..20537)
                     /locus_tag="RPM73_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDX6518705.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20777..20849
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20843..21256
                     /gene="ibpA"
                     /locus_tag="RPM73_000309"
     CDS             20843..21256
                     /gene="ibpA"
                     /locus_tag="RPM73_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDX6518706.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21368..21796
                     /gene="ibpB"
                     /locus_tag="RPM73_000310"
     CDS             21368..21796
                     /gene="ibpB"
                     /locus_tag="RPM73_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDX6518707.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21992..23653
                     /locus_tag="RPM73_000311"
     CDS             21992..23653
                     /locus_tag="RPM73_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDX6518708.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23650..24366)
                     /locus_tag="RPM73_000312"
     CDS             complement(23650..24366)
                     /locus_tag="RPM73_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX6518709.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24662..26278
                     /locus_tag="RPM73_000313"
     CDS             24662..26278
                     /locus_tag="RPM73_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDX6518710.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGLAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26278..27092
                     /locus_tag="RPM73_000314"
                     /pseudo
     CDS             26278..27092
                     /locus_tag="RPM73_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27089..27982)
                     /locus_tag="RPM73_000315"
     CDS             complement(27089..27982)
                     /locus_tag="RPM73_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDX6518711.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28149..29864
                     /locus_tag="RPM73_000316"
     CDS             28149..29864
                     /locus_tag="RPM73_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDX6518712.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFD
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29861..31354
                     /locus_tag="RPM73_000317"
     CDS             29861..31354
                     /locus_tag="RPM73_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDX6518713.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31401..31850)
                     /locus_tag="RPM73_000318"
     CDS             complement(31401..31850)
                     /locus_tag="RPM73_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518714.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31960..32307
                     /locus_tag="RPM73_000319"
     CDS             31960..32307
                     /locus_tag="RPM73_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDX6518715.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32297..32659
                     /locus_tag="RPM73_000320"
     CDS             32297..32659
                     /locus_tag="RPM73_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDX6518716.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32656..33153
                     /locus_tag="RPM73_000321"
     CDS             32656..33153
                     /locus_tag="RPM73_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDX6518717.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33161..34345)
                     /gene="emrD"
                     /locus_tag="RPM73_000322"
     CDS             complement(33161..34345)
                     /gene="emrD"
                     /locus_tag="RPM73_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDX6518718.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(34764..34853)
                     /gene="tisB"
                     /locus_tag="RPM73_000323"
     CDS             complement(34764..34853)
                     /gene="tisB"
                     /locus_tag="RPM73_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDX6518719.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35418..35516
                     /gene="ivbL"
                     /locus_tag="RPM73_000324"
     CDS             35418..35516
                     /gene="ivbL"
                     /locus_tag="RPM73_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDX6518720.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35622..37310
                     /gene="ilvB"
                     /locus_tag="RPM73_000325"
     CDS             35622..37310
                     /gene="ilvB"
                     /locus_tag="RPM73_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDX6518721.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37314..37604
                     /gene="ilvN"
                     /locus_tag="RPM73_000326"
     CDS             37314..37604
                     /gene="ilvN"
                     /locus_tag="RPM73_000326"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDX6518722.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37679..38269
                     /gene="uhpA"
                     /locus_tag="RPM73_000327"
     CDS             37679..38269
                     /gene="uhpA"
                     /locus_tag="RPM73_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDX6518723.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38269..39771
                     /gene="uhpB"
                     /locus_tag="RPM73_000328"
     CDS             38269..39771
                     /gene="uhpB"
                     /locus_tag="RPM73_000328"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDX6518724.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39781..41100
                     /gene="uhpC"
                     /locus_tag="RPM73_000329"
     CDS             39781..41100
                     /gene="uhpC"
                     /locus_tag="RPM73_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDX6518725.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            41238..42629
                     /gene="uhpT"
                     /locus_tag="RPM73_000330"
     CDS             41238..42629
                     /gene="uhpT"
                     /locus_tag="RPM73_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDX6518726.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42674..>44377)
                     /gene="adeD"
                     /locus_tag="RPM73_000331"
     CDS             complement(42674..>44377)
                     /gene="adeD"
                     /locus_tag="RPM73_000331"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDX6518727.1"
                     /translation="AVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVGAE
                     YADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHEIV
                     NVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQVT
                     GLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHESYQ
                     LEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHI
                     DALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVTVQ
                     QVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVIDV
                     IHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGALAATV
                     SHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQS
                     LAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLEVT
                     E"
BASE COUNT        10195 a        11431 c        12065 g        10686 t
ORIGIN      
        1 agggttgagg aataacagga gtgctaacaa tgaagcgaag ccgcatctga cagtcagaat
       61 acggcttcgt aagcgcggtt acttaccaat acagaagctg gagaaaatcc gccccagcag
      121 gtcatcagaa gtaaattccc cggtgatttc gcttaagttc tgctgtgcca ggcgcaactc
      181 ttccgccagc agttcgcccg cccaggcacc taacagttgc gctttacctt gctgcaaatg
      241 ctctgccgcc tgttccagcg cctgtaggtg gcgacggcgc gccaggaagc cgccttccat
      301 gttggtgtca aagcccatgc tctgtttgag atggttacgc agcacatcca caccttcacc
      361 agtccttgcc gagagacgaa ttaacgcgtg accgttcact tcactcattc ccagcgtttc
      421 gccggtgata tcggctttat tgcgcaccac ggtgatcggc agtttcgctg gcagacgggc
      481 gataaattcc ggccagatct ctgccggatc aacggcgtct gtcgtggtgc catcgaccat
      541 aaacagcacg cggtcggcct gttcaatttc ctgccacgcg cgctcgatac caatacgttc
      601 cacttcgtca ctggcttcac gtagcccagc ggtatcgatg atatgcagcg gcattccgtc
      661 aatgtggata tgctcacgca gcacgtcacg tgtggttccg gcgatatcag ttacgattgc
      721 tgcttcacgg cccgccagcg cgtttaacag gctcgattta ccggcgttag gacgtccggc
      781 aatcaccacc ttcatccctt cgcgcaacaa actaccctga cgtgcttcag cacgcactgc
      841 atcgagttcg gcaatcacgt cattaagctg ggcttcaatt ttgccgtcgg agaggaaatc
      901 gatctcttca tcagggaaat caattgccgc ttcgacgtaa atgcgcaagt gggtgagggc
      961 ttccaccaga tggttgaccc gtgcggagaa tgcgccttgc agcgagttaa gtgccgaacg
     1021 ggccgcctgt tccgaactgg cgtcgataag atcggcaatc gcctcggcct gggctaagtc
     1081 gagtttatcg ttaagaaacg cgcgttcgga aaactcacca gggcgagcaa tccgcaggcc
     1141 gggaatggtc agaatgcgtt ttaacagcag gtcgaggatc accggaccgc catgaccttg
     1201 cagttccagc acatcttcgc cggtgaacga gttcgggcca gggaaccata gcgcaatccc
     1261 ctgatcgagc acgctgccgt cggcgtcttt aaacggaaga taatcggcgt agcgcggctt
     1321 aggtagttta cccagcacgg tttcggcaac ttcacgggct ttgaagccgg agatgcgcag
     1381 gatgccaacg ccgccacgtc ccggaggcgt ggcctgggct acgatagtgt cattatcgct
     1441 catgatgttc ctgttgcttt gtgtggcgga tgcgcggtgc ttatccgccc tacgaaaaga
     1501 aaaaaggcgg tcaactgacc gcccttattt tagcgaaaac tcaccgaatc aggatttttt
     1561 cttctcgcgg ctatgcaggc cacgtttttc cagaccacgg taaatcagct gctgctgaat
     1621 aatggttacc aggttgctga cgatatagta cagcaccaga cctgacggga accacaggaa
     1681 gaacacggtg aagatgaccg gcataaaggt catgatcttc tgctgcatcg ggtcggtcac
     1741 tgtggtcggc gacatcttct gaatgaagaa catcgttacg cccatcagga tcggcaagat
     1801 gtagtacggg tcctgtgccg acaggtcatg gatccacagt gcaaacggtg cctgacgcag
     1861 ttcaacggaa cccatcagca tgtagtacaa cgccaggaag attggcatct ggatcagcag
     1921 cgggaagcag ccgcccagcg ggttaacctt ctcagctttg tacagcgcca tcatttcctg
     1981 gctgatacgc tgtttgtcat cacccagacg ctcacgcatt gcctgaatct tcggctgcag
     2041 catacgcatc ttcgccatgg aggtgtactg cgctttggtc agcgggtaca tgatgccacg
     2101 aacgataaag gtgatgatga taatggagaa gccccagtta cccacaaagc tatggatcca
     2161 tttcagcagt ttgaacagcg gctgagagat gaaccacaac caaccgtaat caacggtcag
     2221 atccaggtgc ggagcaacag ctgccatttt gtcctggatt tccgggccaa cccacagggt
     2281 gctgttcatc gcgccagtct gaccaggctg aaccagtacc ggctgagatt tatagccgat
     2341 agcggcgatg ccgttaccca gattagcggt atagaagttg ttggtaccgt cgttatgcgg
     2401 gatccacgcc gtcgcgaaat actgttgcag catcgccacc caaccacctt tcgaagagat
     2461 gttcaggttt tcgttatcgg caatggtatc gaacttgtat ttctcatact tctcgtcagg
     2521 cgtggagtac gccgcgccac ggaaggtgtg cagtgcgaag ttgctgcttc cggtatcgag
     2581 atgcggtggc agagtgatgg attgcttcaa ctgaccaaag gtggagattt ccagcggttt
     2641 ctcgccagcg ttctgcacgt tgtagttgac gttgacagcg taatcaccac gtttcaggac
     2701 aaacgttttg gtaaacgtgt tgcctgccgc gtcggtatac gtcatcggca cctgcagttc
     2761 gttttgacct tcagccagca cataagcgtc tttttcaacg ttatacagcg gacgcgggcc
     2821 gttagccggg ttatccgggc catcacgacc ggtcagaccg ctctgtgcct gataaataaa
     2881 ctgcggtgaa gtttccagca gctggaacgg ctgggtagag ttcagctctt tcgggtaagc
     2941 cggcagcaga gcttgctcaa catcaccacc acgggtgttg atggtcagat caagcacgtc
     3001 ggtcttaacc gagatcagtt tcccctggcc actggccggt acgccctggt cggcggcgct
     3061 acccgctgcg gtggtcgttg tctgcgtggt ctgttgggcc tgaggttgcg ggtttttatc
     3121 ctgctcccag gcttgccaga tcatgaaaga cacgaacagc aaagcgatga ctaaaagatt
     3181 gcgttgcgaa tccatcgtta gtgttctctg gtatcaaatg gtccgggcgg gacgggatcg
     3241 tcaccaccag ggtgtaaagg gtggcatttt aatacgcgtt tcaccgtcaa ccaactgcct
     3301 tttatcactc caaacctgcg caatgcctca attccgtagc ttgaacaggt tggagtgaaa
     3361 cgacaatgcg gcccgagtag cggactaatc aggcgttgat agacccgaat gagggctatc
     3421 aggacccgcg agccaggcga cagtggcggc gccataattt ttccaacgct tccgagagag
     3481 cacggttatc gaggtcggca accccttttt tcgccaccac cacgaaatcc atagccggga
     3541 gttcatgttg gcgcagacgg aagctttcac gcgtcagacg tttaatccga ttgcgttcat
     3601 gggcgcgtcg aacgtttttc ttggcgactg taagaccgat acggggatgc cccagcgaat
     3661 tcaggcggcc gagaatggta atttgcggcg tgccagcccg ttgtggctgc tggaagacga
     3721 atgtgaattg actgggagtt aacaagcgta actccctggg aaatgcgagc ttaaccactc
     3781 aggggttagc tttattactt agaaacggtc agacgagcgc ggcctttagc acgacgacgt
     3841 gccagaacct gacgaccatt tttagtagcc atacgagcac ggaagccgtg agaacggttg
     3901 cgcttcagta cagacggttg aaaagtgcgt ttcatggcga tttctaccta aacttgaata
     3961 aattcaatgg ctttattgga tatccgccga aaaatgaaac gatggacacc gaagccatgg
     4021 gtgattaaag aggccggatt gtaataattg tacactccgg agtcaattct ctttccttat
     4081 ttaccgcgct tttccgcacc ttttcgcagg gaaaatgtac gacctcacac cagtgaaaac
     4141 cagcatggcg cgccgggtgg aggattatac gggctgatgg gtaaagcgca aggatcgtcc
     4201 tggatcttta ttagatcgat taagccaatt tttgtctatg gtcattaaat tttccaatat
     4261 gcggcgtaaa tcgtgcccgc ctcgcggcag gatcgtttac acttagcgag ttctggaaag
     4321 tcctgtggat aaatcgggaa aatctgtgag aaacagaaga tctcttgcgc agtttaggct
     4381 atgatccgcg gtcccgatcg ttttgcagga tcttgatcgg gcatataacc gcagacagcg
     4441 gttcgtgcgt caccctcaag cagggtcttt tcgacgtacg tcaacaatca tgaatgtttc
     4501 agccttagtc attatcgact tttgttcgag tggagtccgc cgtgtcactt tcgctttggc
     4561 agcagtgtct tgcccgattg caggatgagt taccagccac agaattcagt atgtggatac
     4621 gcccattgca ggcggaactg agcgataaca cgctggccct gtacgcgcca aaccgttttg
     4681 tcctcgattg ggtacgggac aagtacctta ataatatcaa tggactgcta accagtttct
     4741 gcggagcgga tgccccacag ctgcgttttg aagtcggcac caaaccggtg acgcaaacgc
     4801 cacaagcggc agtgacgagc aacgtcgcgg cccctgcaca ggtggcgcaa acgcagccgc
     4861 aacgtgctgc gccttctacg cgctcaggtt gggataacgt cccggccccg gcagaaccga
     4921 cctatcgttc taacgtaaac gtcaaacaca cgtttgataa cttcgttgaa ggtaaatcta
     4981 accaactggc gcgcgcggcg gctcgccagg tggcggataa ccctggcggt gcctataacc
     5041 cgttgttcct ttatggcggc acgggtctgg gtaaaactca cctgctgcat gcggtgggta
     5101 acggcattat ggcgcgcaag ccgaatgcca aagtggttta tatgcactcc gagcgctttg
     5161 ttcaggacat ggttaaagcc ctgcaaaaca acgcgatcga agagtttaaa cgctactacc
     5221 gttccgtaga tgcactgctg atcgacgata ttcagttttt tgctaataaa gaacgatctc
     5281 aggaagagtt tttccatacc ttcaacgccc tgctggaagg taatcaacag atcattctca
     5341 cctcggatcg ctatccgaaa gagatcaacg gcgttgagga tcgtttgaaa tcccgcttcg
     5401 gttggggact gactgtggcg atcgaaccgc cagagctgga aacccgtgtg gcgatcctga
     5461 tgaaaaaggc cgacgaaaac gacattcgtt tgccgggcga agtggcgttc tttatcgcca
     5521 agcgtctacg atctaacgta cgtgagctgg aaggggcgct gaaccgcgtc attgccaatg
     5581 ccaactttac cggacgggcg atcaccatcg acttcgtgcg tgaggcgctg cgcgacttgc
     5641 tggcattgca ggaaaaactg gtcaccatcg acaatattca gaagacggtg gcggagtact
     5701 acaagatcaa agtcgcggat ctcctttcca agcgtcgatc ccgctcggtg gcgcgtccgc
     5761 gccagatggc gatggcgctg gcgaaagagc tgactaacca cagtctgccg gagattggcg
     5821 atgcgtttgg tggtcgtgac cacacgacgg tgcttcatgc ctgccgtaag atcgagcagt
     5881 tgcgtgaaga gagccacgat atcaaagaag atttttcaaa tttaatcaga acattgtcat
     5941 cgtaaaccta tgaaatttac cgtagaacgt gagcatttat taaaaccgct acaacaggtg
     6001 agcggtccgt taggtggtcg tcctacgcta ccgattctcg gtaatctgct gttacaggtt
     6061 gctgacggta cgttgtcgct gaccggtact gatctcgaga tggaaatggt ggcacgtgtt
     6121 gcgctggttc agccacacga gccaggagcg acgaccgttc cggcgcgcaa attctttgat
     6181 atctgccgtg gtctgcctga aggcgcggaa attgccgtgc agctggaagg tgaacggatg
     6241 ctggtacgct ccgggcgtag ccgtttttcg ctgtctaccc tgccagcggc ggatttcccg
     6301 aacctcgatg actggcagag tgaagtcgaa tttaccctgc cgcaggcaac gatgaagcgt
     6361 ctgattgaag cgacccagtt ttctatggcg catcaggacg ttcgctatta cttaaatggt
     6421 atgctgtttg aaaccgaagg tgaagaactg cgcaccgtgg caaccgacgg ccaccgtctg
     6481 gcggtctgtt caatgccaat tggtcaatct ttgccaagcc attcggtgat cgtaccgcgt
     6541 aaaggcgtga ttgaactgat gcgtatgctc gacggcggcg acaatccgct gcgcgtacag
     6601 attggcagca acaacattcg cgcccacgtt ggcgacttta tcttcacctc caaactggtg
     6661 gatggtcgct tcccggatta tcgccgcgtt ctgccgaaga acccggacaa acatctggaa
     6721 gctggctgcg atctgctcaa gcaggcgttt gctcgcgcgg cgattctctc taacgagaaa
     6781 ttccgcggcg tacgtcttta tgtcagcgaa aaccagctga aaatcaccgc caacaacccg
     6841 gaacaggaag aagcggaaga gatcctcgac gttacctata gcggtgcgga gatggaaatc
     6901 ggcttcaacg tcagctatgt gctggatgtt ctgaacgcgc tgaaatgcga aaacgtccgc
     6961 atgatgctga ccgattcggt ttccagcgtg cagattgaag atgccgcatc acagtcggct
     7021 gcctatgttg tcatgccaat gagactgtaa tgtccctcac ccgcttgttg atccgcgatt
     7081 tccgcaacat tgaaaccgcg gatctcgctt tatctcccgg ctttaacttt ctggtaggtg
     7141 ccaacggcag tggcaaaacc agcgtgctgg aagccatcta tacgctcggc catggtcggg
     7201 cgtttcgcag tttgcagatt ggtcgcgtca ttcgccatga gcaggaggca tttgttctcc
     7261 atgggcgatt acagggcgaa gagcgcgaga cggcgattgg cttaaccaag gacaaacagg
     7321 gcgacagcaa agtccgcatc gacggtactg acgggcataa agtcgcggaa ctggcgcacc
     7381 tgatgccaat gcagctgata acgccagaag ggtttacttt actcaacggc ggccccaaat
     7441 acagaagagc attcctcgac tggggatgct ttcacaacga acccggattt ttcaccgcct
     7501 ggagcaatct caagcgattg ctcaagcagc gcaatgcggc gctgcgccag gtgacacgtt
     7561 acgaacagct acgcccgtgg gataaagaac tgatcccgct ggcggagcaa atcagcacct
     7621 ggcgcgcgga gtatagcgcc ggtatcgcgg ccgatatggc cgatacctgt aagcaatttc
     7681 tccctgagtt ttctctgact ttctctttcc agcgcggctg ggagaaagag acagaatatg
     7741 ctgaggtgct ggaacgtaat tttgaacgcg atcgccagct aacctacacc gcgcatggcc
     7801 cgcataaagc ggacttacgc attcgcgccg acggtgcgcc ggtggaagat accttatcgc
     7861 gtgggcagct taagctgttg atgtgcgcct tacgtctggc gcaaggagag ttcctcaccc
     7921 gtgaaagcgg gcggcggtgt ctctacctga tagatgattt tgcctctgag cttgatgatg
     7981 agcgtcgcgg gctgcttgcc agccgcttaa aagcgacgca atcacaggtc tttgtcagcg
     8041 cgatcagtgc tgaacacgtt atagacatgt cggacgaaaa ttcgaagatg tttaccgtgg
     8101 aaaagggtaa aataacggat taacccaagt ataaatgagc gagaaacgtt gatgtcgaat
     8161 tcttatgact cctccagtat caaagtcctg aaagggctgg atgcggtgcg taagcgcccg
     8221 ggtatgtata tcggcgacac ggatgacggc accggtctgc accacatggt attcgaggtg
     8281 gtagataacg ctatcgacga agcgctcgcg ggtcactgta aagaaattat cgtcaccatt
     8341 cacgccgata actctgtctc tgtacaggat gacgggcgcg gcattccgac cggtattcac
     8401 ccggaagagg gcgtatcggc ggcggaagtg atcatgaccg ttctgcacgc aggcggtaaa
     8461 tttgacgata actcctataa agtgtccggc ggtctgcacg gcgttggtgt ttcggtagta
     8521 aacgccctgt cgcaaaaact ggagctggtt atccagcgcg agggtaaaat tcaccgtcag
     8581 atctacgaac acggtgtacc gcaggctccg ctggcggtta ccggcgagac tgaaaaaacc
     8641 ggcaccatgg tgcgtttctg gcccagcctc gaaaccttca ccaatgtgac cgagttcgaa
     8701 tatgaaattc tggcgaaacg tctgcgtgag ttgtcgttcc tcaactccgg cgtttccatt
     8761 cgtctgcgcg acaagcgcga cggcaaagaa gaccacttcc actatgaagg cggcatcaag
     8821 gcgttcgttg aatatctgaa caagaacaaa acgccgatcc acccgaatat cttctacttc
     8881 tccactgaaa aagacggtat tggcgtcgaa gtggcgttgc agtggaacga tggcttccag
     8941 gaaaacatct actgctttac caacaacatt ccgcagcgtg acggcggtac tcacctggca
     9001 ggcttccgtg cggcgatgac ccgtaccctg aacgcctaca tggacaaaga aggctacagc
     9061 aaaaaagcca aagttagcgc caccggtgac gatgcgcgtg aaggcctgat tgcggtcgtt
     9121 tccgtgaaag tgccggaccc gaaattctcc tcccagacca aagacaaact ggtttcttct
     9181 gaggtgaaat cagcggttga acagcagatg aacgaactgc tggcagaata cctgctggaa
     9241 aacccaaccg acgcgaaaat cgtggttggc aaaattatcg atgctgcccg tgcccgtgaa
     9301 gcggcgcgtc gcgcgcgtga aatgacccgc cgtaaaggtg cgctcgactt agcgggcctg
     9361 ccgggcaaac tggcagactg ccaggaacgc gatccggcgc tttccgaact gtacctggtg
     9421 gaaggggact ccgcgggcgg ctctgcgaag caggggcgta accgcaagaa ccaggcgatt
     9481 ctgccgctga agggtaaaat cctcaacgtc gagaaagcgc gcttcgataa gatgctctct
     9541 tctcaggaag tggcgacgct tatcaccgcg cttggctgtg gtatcggtcg tgacgagtac
     9601 aacccggaca aactgcgtta tcacagcatc atcatcatga ccgatgcgga cgtcgacggc
     9661 tcgcacattc gtacgctgct gttgaccttc ttctatcgtc agatgccgga aatcgttgaa
     9721 cgcggtcacg tctacatcgc tcagccgccg ctgtacaaag tgaagaaagg caagcaggaa
     9781 cagtacatta aagacgacga agcgatggat cagtaccaga tctctatcgc gctggatggc
     9841 gcaacgctgc acaccaacgc cagcgcaccg gcattggctg gcgaagcgtt agagaaattg
     9901 gtgtctgagt acaacgcgac gcagaaaatg atcaaccgca tggagcgtcg ttatccgaaa
     9961 gcaatgctga aagagcttat ctatcagccg acgctgacgg aagccgacct ctctgatgag
    10021 cagaccgtta cccgctgggt gaacgcgctg gtcagcgaac tgaacgacaa agaacagcac
    10081 ggcagccagt ggaagtttga tgtccacacc aatgccgaac aaaacctgtt cgagccgatt
    10141 gttcgcgtgc gtacccacgg tgtggatact gactatccgc tggatcacga atttatcact
    10201 ggtggcgaat atcgtcgtat ctgcacgctg ggtgagaaac tgcgtggctt gctggaagaa
    10261 gatgcattta tcgaacgtgg cgagcgtcgt cagccggtag ccagcttcga gcaggcgctg
    10321 gactggctgg tgaaagagtc tcgtcgcggt ctctccatcc agcgttataa aggtctgggc
    10381 gagatgaacc cggaacagct gtgggaaacc accatggacc cggaaagtcg tcgtatgctg
    10441 cgcgttaccg ttaaggatgc gattgccgct gaccagttgt tcaccacgct gatgggcgac
    10501 gccgtagaac cgcgtcgtgc gtttattgaa gagaacgccc tgaaagcggc gaatattgat
    10561 atttaatggc gttaaccatg cgagcgtgcc tgatgcgcta cgcttattag tcctacgaga
    10621 ctcctgcaat atattgattt tgcatgattt tgtaggccgg ataaggcgtt cacgccgcat
    10681 ccggcaagaa gctataagaa aagggcggag atcatctccg ccctttttat ttctgcaatc
    10741 cgcaattatc atcccgcaaa tctatacttt ttgggtcaac acgtaacgct ttacaggtga
    10801 ttatcatggg gctttttgat gaagttgtcg gtgcctttct gaaaggcgat gcggggaaat
    10861 atcaggctat tttaagttgg gttgaggagc agggcggcat tcaggtgctg ctggaaaaac
    10921 tgcaaagtgg cggcttaggg gccattctct caacctggct gagtaatcaa cagggcaatc
    10981 aatcggttag tggcgagcaa ctggaatcgg cgctcggcac aaatgcggtg tccgatctcg
    11041 ggcaaaaact tggcgtggat accagtacag cttccagttt actggcagag caattgccga
    11101 agattattga tgcgctctca ccgcaaggtg aagtgtcacc acaagccaat aacgatctgc
    11161 tttccgcagg tatggaactg ctgaaaggga aactcttccg ctaagcaaat ggggagcatg
    11221 caacccgcgt tgcatccccc ttttccctcg ggtgttgtac tgatttttga gcggaatcgc
    11281 gttagcatgg gtcaggaacc aatctacctg gggaactcat ggctatcaaa ctcattgcta
    11341 tcgatatgga tggcaccctt ctgctgcccg atcacaccat ttcacccgcc gttaaaaatg
    11401 cgattgccgc agctcgcgcc cgtggcgtga atgtcgtgct aacgacgggt cgcccttatg
    11461 caggcgttca caactacctg aaagagctgc atatggaaca gccgggcgac tactgcatta
    11521 cttataacgg cgcgttggta cagaaggccg ccgatggtag caccgtggcg caaaccgctc
    11581 tcagctatga cgactaccgt ttcctggaaa aactctctcg cgaagtcggt tctcatttcc
    11641 acgccctgga ccgcaccacg ctgtatacag caaatcgcga tatcagctac tacacggtgc
    11701 atgaatcctt cgttgccacc attccgctgg tgttctgcga agcggagaaa atggacccca
    11761 atacccagtt cctgaaagtg atgatgattg atgaacccgc catcctcgac caggctattg
    11821 cgcgtattcc gcaggaagtg aaagagaaat ataccgtgct gaaaagtgcg ccgtacttcc
    11881 tcgaaatcct cgataaacgc gttaacaagg gtacgggtgt gaaatcactg gccgacgtgt
    11941 taggtattaa accggaagaa atcatggcga ttggcgatca ggaaaacgac atcgcaatga
    12001 ttgaatatgc aggcgtcggt gtggcgatgg ataacgctat tccttcggtg aaagaagtgg
    12061 cgaactttgt caccaaatcc aaccttgaag atggcgtggc gtttgctatt gagaagtatg
    12121 tgctgaatta atcagtggac gggcaaacag cataaacgcg tttgcccgct tactgattat
    12181 tggtttactg catatacaac cagaaattgc agcggtgcag taatgatgtc cagcgtaaca
    12241 gtcactggca gtaatgctgc ggaaacacca tccggaatgc ctctgggtga atagtattca
    12301 taaaatcgca ctttaaatgg atggtagaac gccatcacct ttgagtaatc catgttttta
    12361 tttttctcat ggattgtgcc ttttaggttt tccagtgacc tggtacagtg ttgaacacca
    12421 caggcaaaac cataatgcgt tgccagtgcc ttttcttctt cgttattcca gtagtagtaa
    12481 agcgatattg tgccggtaaa tttctttttc cccgcattta aaacaaatct tgcgtcatcg
    12541 gcaacctgaa tattgtgccg gttaagtgct ggatcattga gcatcttaac gatgtcatcg
    12601 gcaccatcag tgataaggta atccagcgat tcccctacaa aaacatagcc ttttgttcca
    12661 ttactatctt ttgcctgcga tagcccagtg atggtgtctg actgcgattg atactttcct
    12721 gaactaccct ttgcggtatg ggaaaccaga gcatacgaga tacagcctga aagcattagc
    12781 gccagtggga ataaaccggc agccttaaaa aatcccttaa gattcaactt catgattaat
    12841 cctttaaacg agtagctaag ccgttgaaag catactcttc tttccctccc atttagcgcc
    12901 tgatatccct tttcagattt ctgcccgacg catgtcattt ttttatgcat tgttcttttt
    12961 gtgatctaaa ttgtagtaca acaatataag tttgtactac attacgcgca cggcaaacgc
    13021 gaacgtcatc acgctggtac tacaaagttg ccgcgttatg catcgatcgg ggtaaagtag
    13081 agaagaacat acagagcaca aggactctcc atgactctca ataaaaccga tcgcattgtc
    13141 attacgctgg gtaaacagat cgttcacggc aaatacgtgc caggctcgcc gcttccggct
    13201 gaggcggaac tctgtgagga gtttgcaacc tcgcgcaaca tcatccgtga ggtgttccgt
    13261 tcgctgatgg cgaagcggct gattgaaatg aaacgttatc gcggggcgtt tgtggcaccg
    13321 cgtaaccagt ggaattacct cgacactgac gtactgcaat gggtgctgga aaacgactac
    13381 gatccacggc ttatcagtgc catgagcgaa gtgcgaaatc tggtggaacc ggcgattgcc
    13441 cgttgggcag cagagcgcgc gacttccagc gatctggcgc agattgaatc ggcgctgaac
    13501 gagatgattg ccaacaatca ggaccgcgaa gcgtttaacg aagcggatat tcgctaccac
    13561 gaggcggtgc tgcagtcggt gcataacccg gtgttacagc aacttagcat tgcgatcagt
    13621 tcgctgcagc gggcggtttt tgaacgaacc tggatgggcg atgaggccaa catgccgcaa
    13681 acgctccagg aacataaggc gctgttcgat gcgatacgac atcaggacgg cgatgcggca
    13741 gagcaggcgg cgcttaccat gatcgccagc tcgacacgaa ggttaaagga aatcacatga
    13801 cagctcgcta catcgcaatt gactggggat cgaccaatct gcgtgcctgg ctttatcagg
    13861 gcgaccactg cctggacagc aggcaatcag aagcaggcgt cacgcgcctg aacggaaaat
    13921 ctccggctgc ggtgttagca gaagtcacga ccgactggcg tgaagagaat acgccagtgg
    13981 tgatggcagg aatggtcggc agcaatgtcg gctggaaagt tgctccgtat ttatctgttc
    14041 ctgcccgttt ttcgtctatt ggcgaacaat taacgtctgt tggcgacaat atctggatta
    14101 ttcccggatt atgcgtctct catgacgata accacaatgt gatgcgcggc gaagaaacac
    14161 aattgatcgg cgcgcgaact ctggctcctt cctctcttta tgttatgccc ggtacgcatt
    14221 gcaaatgggt gcaggccgat agccagcaaa tcaacgattt tcgcaccgtg atgaccggtg
    14281 aactacatca tttactgtta aatcactcat tgattggcgc aggtttgccg ccgcaggaaa
    14341 actctgccga tgccttcgcg gctggccttg agcgcggcct taatgcgccc gccatattgc
    14401 cgcagctttt tgaagttcgc gcctcgcatg tgctgggaac acttccccgc gaacaggtca
    14461 gcgaatttct ctctggtttg ttgattggcg cagaggttgc cagtatgcgc gactatgtga
    14521 cccatcaaca cgccatcacc cttgtcgccg gaacatcgct gaccgcgcgc taccagcaag
    14581 cctttcaggc gatgggttgc gacgtgacgg cggtggcggg cgacacggca tttcaggctg
    14641 gtataaggag catcgctcat gcagtggcaa actaaacttc cgctgatcgc cattttgcgc
    14701 ggcattaagc ccgacgaggc gctggcgcat gttggcgcgg tgattgacgc cgggttcgac
    14761 gcggttgaaa tcccgctgaa ttccccacaa tgggagcaaa gcattcccgc catcgttgat
    14821 gcgtatggcg acaaggcgtt gattggcgca ggtacggtac tgaaacctga acaggtcgat
    14881 gcgctcgcca ggatgggctg tcagctcatc gttacgccca atatccatag tgaagtgatc
    14941 cgccgtgcgg tgggctacgg catgaccgtc tgccccggct gcgcgacggc gaccgaagcc
    15001 tttaccgcgc tcgaagcggg cgcgcaggcg ctgaaaatat ttccgtcatc ggcttttggt
    15061 ccgcaataca tcaaagcgtt aaaagcggta ttgccatcgg acatcgcagt ctttgccgtt
    15121 ggcggcgtga cgccagaaaa cctggcgcag tggatagacg caggttgtgc tggggcgggc
    15181 ttaggcagcg atctctatcg cgccgggcag tccgtagaac gcaccgcgca gcaggcagca
    15241 gcatttgtta aggcgtatcg agaggcagtg caatgaaaat caccaaaatt accacgtatc
    15301 gtttacctcc ccgctggatg ttcctgaaaa ttgaaaccga tgaaggcgtg gtcggttggg
    15361 gcgagcccgt gattgaaggc cgcgcccgta cggtggaagc ggcagttcac gagctgggtg
    15421 actatttgat tggtcaggat ccttcgcgca tcaatgactt atggcaagtg atgtatcgcg
    15481 ccggatttta tcgtggcggt ccaatcctga tgagcgccat tgccgggatc gaccaggcgt
    15541 tatgggatat caaaggcaaa gtgctgaatg cgccggtctg gcaactgatg ggcggcctgg
    15601 ttcgcgacaa aattaaagcc tacagttggg tcggcggcga tcgtccggcg gatgttatcg
    15661 acggcattaa aaccctgcgc gaaatcggct tcgatacctt caaactgaac ggttgtgaag
    15721 aactggggct aattgataac tcccgcgcgg tagatgcggc agtcaacacc gtggcacaaa
    15781 ttcgtgaagc ttttggcaat cagattgagt ttggtcttga tttccacggt cgcgtcagcg
    15841 cgccaatggc gaaagtgctg attaaagaac tggagccgta tcgcccgctg tttattgaag
    15901 agccggtgct ggcggaacaa gccgaatact acccgaaact ggcggcacaa acgcatattc
    15961 cactggcggc gggtgagcgc atgttctcac gtttcgattt taaacgcgtg ctggaggcag
    16021 gcggtatttc gattctgcaa ccggatctct cccatgcagg cggtattacc gaatgctaca
    16081 aaattgctgg aatggcagaa gcctatgatg tgacccttgc gccgcactgt ccgctcggac
    16141 cgattgcact ggcggcttgc ctgcatatcg actttgtttc ctataacgcg gtacttcagg
    16201 aacaaagtat gggcattcat tacaacaaag gcgcggagtt actcgacttt gtgaaaaaca
    16261 aagaagactt cagtatggtt ggcggcttct ttaaaccgtt aacgaaaccg ggcttaggtg
    16321 tggaaatcga cgaagctaaa gttattgagt tcagtaaaaa tgccccggac tggcgtaatc
    16381 cgctctggcg tcatgaagat aacagcgtag cagagtggta attcctgcca cgtaagcccc
    16441 tcatcgggca ctaaaacagc aatacaaaaa tataaccctc tgtaaattac agggcatggt
    16501 gagcggcttc gctatgccca aaatctggag aaagctcgct atggatattc ccgttaatgc
    16561 agcaaagccg gggcgtcggc gttatctgac gctggtgatg atctttatta cggtggtcat
    16621 ttgttatgtt gaccgcgcta acctggccgt ggcttccgcc catattcagg aagagttcgg
    16681 cattacgaaa gcggaaatgg gctatgtatt ttcggccttc gcctggcttt atacgctatg
    16741 ccagatcccc ggcggttggt ttttagatcg cgtaggttct cgcgtgactt attttattgc
    16801 gatatttggt tggtcagtgg cgactttatt ccagggcttt gccacgggat taatgtcatt
    16861 aattggtctg cgtgcgataa ccggtatttt cgaagcgccc gctttcccga ccaataaccg
    16921 gatggtgacc agctggttcc cggaacatga acgcgcttcc gccgttggtt tttatacgtc
    16981 tggtcagttt gtcggtctgg cgtttttgac tccgctgctg atctggattc aggagatgtt
    17041 gagctggcac tgggtgttca ttgtcactgg tggtatcggc attatctggt cgctgatttg
    17101 gtttaaggtt tatcagccgc cgcgcctgac caaaggtatc agcaaagctg aactggatta
    17161 cattcgtgat ggcggcggtc tggtggatgg tgatgcgccg gtgaagaaag aggcgcgtca
    17221 gccgttaaca gccaaagact ggaaactggt gttccatcgt aaactgatcg gcgtttatct
    17281 tgggcaattt gcggtggctt ctacactgtg gtttttctta acctggttcc cgaactattt
    17341 aacccaggaa aaaggaatca cggcgctgaa agcaggcttt atgaccacgg tgccattcct
    17401 cgcggcgttt gtcggcgtcc tgctctctgg ctgggtcgcg gatctgctgg tacgtaaggg
    17461 cttttcactg ggctttgcgc gtaaaacgcc gattatctgc ggcttgctga tctccacctg
    17521 cattatgggc gctaactaca ccaacgatcc gatgatgatt atgtgcctga tggcgctggc
    17581 attcttcggc aacggttttg cttcgattac ctggtcgctg gtctcttctc tggcaccgat
    17641 gcgcctgatt ggtttaaccg gcggcgtgtt taacttcgcc ggtggtctgg gcggcatcac
    17701 cgttccgctg gtggtggggt acctggcgca gggttacggt ttcgcacctg cactggttta
    17761 tatctccgcc gtcgcgttga ttggcgcgct ctcttacatc ctgctggtgg gcgatgtgaa
    17821 gcgcgttggc taatctttca actgtggaat atgcgccata ccactgcgca taatccactt
    17881 tcttaaagcc ggtgcggtca ggcatcggct ttttactatc ttgccgaaga gttttaaacg
    17941 cagtttgcgg gtggcgcgcc agtaagcggt attgagcttc tctggctgct tcagtaacac
    18001 cgaacgcaga atgtctgcgc tatccagcgc atagctaatc ccctccagcg aactggcgct
    18061 gataaatcct gccgcttcgc caatcagaaa ggcgttgtcc ttaccgcaga caaaatcctg
    18121 ccagcgtgag ggaaacagca ccgtgcattt ttcgcttttc accgccttac caaactggaa
    18181 ctgaaaggcg ctcattttct ctttcagcgt cgtgaaacgc gtctgaccgt ctttcattgg
    18241 ataagcaccg ccaaagataa aataaccgtc tttgctgata ctccatgaat aacagttagt
    18301 tatcgaatta tcaaagatgc aggagtagaa cggcaccgga tgtttctctg cgaaccactg
    18361 ctggatagcg acatatttac ggatttgatg atccgggtag agatgtcgcc gcaccatcga
    18421 gtttgcgcca tcggcaccga ccagatagcg ggcagtaata tgttgctccc agccgtctgc
    18481 acgaaaaatg acatgccatt tatcatcctc acgccagatt tttcggcaca ggctatcgtg
    18541 ataaacctca acgctggcgg ggatcagcga tttcatccac aagtcgaaag catggcgatt
    18601 gatatagctt cgctggtagt tacgcgtgag cgatgcggcg acgtcgacag ttttgacgct
    18661 gaaaatctgc gggttggcga tcacatcgac aggaagcgtc agtccatcgc gaataaaaga
    18721 tcgctgcgcg tccggtgcca gcagaccgcc gcaaggtttg ctgaaacctt cagtaccaca
    18781 ctggtgcttt ttatccagcg cgatcacctg cattttgcct gctaattttc gcgccaacgc
    18841 cgatcctgcc gggccgaggc caataatcgc cacgtcgaaa tgttccatct tttcactcac
    18901 attcatcacg atggcaaaag actattgtct gaaagtgaag tccttgtgaa gtatgatggc
    18961 gggaaccgtt ttgtatcagg accgcgccat gaaacagata acctttgctc cccgtaatca
    19021 cctgctcacc aataccaata cctggacgcc cgacagccag tggctggtat ttgacgtgcg
    19081 tccttctggg gcgtcgttta ccggcgagac cattgagcgt gtgaatatcc ataccggcga
    19141 ggtcgaggtt atctatcgcg cgtcacaggg cgcacacgtc ggcgtggtga ccgttcatcc
    19201 gaagtcagag aaatatgtct ttattcacgg ccctgaaaat cctgatgaaa catggcatta
    19261 cgacttccat catcgacgcg gagtcattgc tgaaggcggc aaggtgagca atctcgatgc
    19321 aatggatatt accgcgccgt atacgccagg agcgctgcgc ggcggcagcc atgtgcatgt
    19381 ctttagcccg aacggtgaaa gggtgagctt tacctataac gaccatgtaa tgcatgaact
    19441 cgatccggcg ctggatttgc gaaacgtcgg tgttgctgcg ccgtttggcc cggtcaacgt
    19501 acaaaagcag catccgcgtg aatacagcgg tagccactgg tgcgtgctgg tgagtaaaac
    19561 cacgcccacg ccgcagcctg gcagcgatga aatcaatcgt gcttatgaag aaggatgggt
    19621 aggaaatcac gcgctggcgt ttattggcga cacactttcg ccaaagggcg agaaagtgcc
    19681 ggagctgttt atcgttgagt taccgcaaga tgaagctagc tggaaagcag caggtgatgc
    19741 gccgttaagt ggaacggaaa ccaccctgcc cgcgccaccg cgcggcgtcg tgcagcgacg
    19801 tttaaccttt acccaccatc gggcttatcc ggggttagtc aacgtcccgc gccactgggt
    19861 gcgctgtaat ccgcagggta cgcaaatcgc gtttttaatg cgtgatgata acggcattgt
    19921 gcaactgtgg cttatctcgc cacagggcgg cgagccgcgc cagttaaccc ataacaaaac
    19981 ggatattcag tctgcattta actggcatcc gtcaggagaa tggttgggct ttgtgctgga
    20041 taatcgaatt gcttgtgccc atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca
    20101 cgccaatcca ccttctgcgg acgccgtggt cttctcgccg gatggtcagt ggctggcgtg
    20161 gatggaaggc ggccagctgt ggatcaccga aactgatcgc taaatcagtt atcaggcatt
    20221 ctggccggtg gaataaccgc attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt
    20281 gagcgcaccg aactgtcttt gcggaacaca tcccacggca gtaaaagcgt gtccatcaca
    20341 gcggtaaacg gcatatcgag aatcgccagc gacttagtgc cccagttggt ttcatcgtcg
    20401 ccaatcatgg ttgcgctggc gcgggtgccg ggatacgttc cttctttccc tccggtatgg
    20461 gacatcacgc tggagcatcc tccgagtaat gtcattccgc tgcatatcat gaacgctaac
    20521 aacacatttc ttatcataat aaattcatct gttgatcgtg ggtgttggcc tgatgagtta
    20581 tagcgatccc ttgctgaaaa taacatcatc attacgtcgc actgtggcgg ctatcgcact
    20641 ttaacgtttc gtgctgcccc ctcagtctat gcaatagacc ataaactgca aaaaaaagtc
    20701 cgctgataag gcttgaaaag ttcatttcca gacccatttt tacatcgtag ccgatgagga
    20761 cgcgcctgat gggtgttctg gctacctgac ctgtccattg tggaaggtct tacattctcg
    20821 ctgatttcag gagctattga ttatgcgtaa ctttgattta tccccgcttt accgttctgc
    20881 tattggattt gaccgtttgt ttaaccactt agaaaacaac cagagccaga gtaatggcgg
    20941 ctaccctccg tataacgttg aactggtaga cgaaaaccat taccgcattg ctatcgctgt
    21001 ggctggtttt gctgagagcg aactggaaat taccgcccag gataatctgc tggtggtgaa
    21061 aggtgctcac gccgacgaac aaaaagagcg cacctatctg taccagggca tcgctgaacg
    21121 caactttgaa cgcaaattcc agttagctga gaacattcat gttcgtggcg ctaacctggt
    21181 aaatggtttg ctgtatatcg atctcgaacg cgtgattccg gaagcgaaaa aaccgcgccg
    21241 tatcgaaatc aactaattcc ctaaggccgc ctggcgcggc ctgacatctc catgctcgcc
    21301 gtcagggagc atatgcgaat cttcggattt gcaggtactt actcgcttct tagaaggaga
    21361 aatgactatg cgtaacttcg atttatcccc actgatgcgt caatggatcg gttttgacaa
    21421 actggccaac gcactgcaaa acgccggtga aagccagagc ttcccgccgt acaacattga
    21481 gaaaagcgac gataaccact accgcattac ccttgcgctg gcaggtttcc gtcaggaaga
    21541 tttagagatt caactggaag gtacgcgcct gagcgtaaaa ggcacgccgg agcagccaaa
    21601 agaagagaaa aaatggctgc atcaagggct tatgaatcag ccatttagcc tgagctttac
    21661 gctggctgaa aatatggaag tctctggcgc aaccttcgta aacggtttac tgcatattga
    21721 tttaattcgt aatgagcctg aacccatcgc agcgcagcgt atcgctatca gcgaacgtcc
    21781 cgcgttaaat agctaactag ctattctctt tgccccgcca ttctggtggg gctttttttg
    21841 tctttcttct tcaacaatgc gttaaatttc gcctgtttaa gatatttcgg cacgttcttt
    21901 cccgtgataa tgtgcgctat aaggcaaatc tgcttcaccg attcagccat aatccgtagc
    21961 aattaaaatg ttatctcaag agaaatggac gatgagtgat atagcattaa cggtcagtat
    22021 tctggctttg gtggcagtcg tcggtttgtt tatcggcaac gtcaaatttc gcggcatagg
    22081 attaggtatt ggcggcgtgc tgtttggtgg gatcatcgtc ggccattttg tttctcaggc
    22141 ggggatgaca ttaagtagcg atatgctgca tgttattcag gaatttggcc tgatcctgtt
    22201 cgtttatact atcgggattc aggtagggcc gggcttcttt gcctcattgc gcgtctccgg
    22261 attacgcctc aacctgtttg ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat
    22321 cctgcataaa ctgtttgata ttccactgcc ggtagtgctg gggattttct ccggtgcggt
    22381 taccaatacg ccagcgctgg gggcagggca gcagattttg cgcgacctgg gtacaccaat
    22441 ggaaatggtc gatcagatgg ggatgagtta cgcgatggcg tatccattcg gcatttgcgg
    22501 gattttgttc accatgtgga tgttgcgggt tattttccgc gtcaatgtcg agacagaagc
    22561 tcagcagcac gagtcttcac gcaccaatgg cggcgcgctg atcaagacta tcaatattcg
    22621 cgttgagaac cctaacctgc atgatttagc cattaaagat gtaccgattc tcaacggcga
    22681 caaaattatc tgctcgcgtc tgaaacgcga agaaacccta aaagttcctt cgccagatac
    22741 cattattcaa ctgggcgatt tgctgcatct ggtgggtcag ccagcggatt tacataatgc
    22801 gcaactggtg attggtcagg aggtcgatac ttcgctgtcc acgaaaggca ctgatttgcg
    22861 cgtcgagcgt gtggtggtca ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca
    22921 ctttaaagaa cgctatgacg ttgttatctc gcgcctgaac cgtgccgggg tcgaactggt
    22981 cgccagtggc gatatcagcc tgcagttcgg cgatatcctc aatctggtgg ggcgtccgtc
    23041 cgcaattgat gccgttgcca atgtgctggg gaatgcgcag caaaaactgc aacaggttca
    23101 gatgctgcca gtgtttattg gcatcgggct tggcgtattg ttaggttcta ttcccgtctt
    23161 tgtgcctggg ttcccggccg cgttgaaact ggggctggcg ggcggtccgc tgattatggc
    23221 gttgatcctc gggcgtatcg gcagtatcgg caagctgtac tggtttatgc cgccaagtgc
    23281 caacctcgcg ctgcgggagc tggggatcgt gctgttcctc tcggtcgttg gtctgaaatc
    23341 tggtggggat tttgtaaata ccctggtcaa tggcgaaggg ctaagctgga ttggttatgg
    23401 tgccctgatc accgccgttc cgctgattac tgttggcatt ctggcgcgga tgttagccaa
    23461 aatgaattac ctgaccatgt gcgggatgct ggcaggttcc atgaccgatc ctccggcgct
    23521 ggcgtttgct aataatcttc atccaaccag cggtgcggcg gcgctctctt acgccactgt
    23581 ctatccgtta gtgatgttcc tgcgcattat caccccccaa ttactggcgg tgctcttctg
    23641 gagtatcggt taactctttt ccgggtggag tcgccgtaaa tggtactcca cctggtattc
    23701 gctggcattt ctgaacatca ctgaataatt caaaaactcc ccgctctcgc tatatgacaa
    23761 cgaggtgatc cgcagcagtg gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct
    23821 atcggcgagc actggcgtca ggctttcata attaccgcca atcaaaatcc cgcactcttg
    23881 ttcaatatat tcaaacttcg acccttccag atgttgcagc gaaagattac ggaacagttt
    23941 taccggcata tagctgtctt ccagcatcag cggtttccct tccacaaaac gaacgcgacg
    24001 ggagaagtag atctgctcgt tgatttgaat ccgtaactgg ctggcaatgg ccggaggcgc
    24061 aggcatgatt tcaaaaatca gcacctggct ggtgaccgtt tttccctgcc gttttaacac
    24121 ctccaccagc ccggtcaggc tggcggtttg atgcagtaca tctttgcgca ccaggtaagt
    24181 gccgctgccg tggcggcgga ccaccaatcc ccacgctacc agcaggtcaa tggctttacg
    24241 gatggtcatc cgcgataccg caaattcttc cgccaacttt ttttcaccgg gaagaaggct
    24301 atttagcgtg aaatctgcgg aattaagtcg aattcttaac cgctccgcaa tgcttttgta
    24361 gatcatctct agatctcttt tttgcctgca agcacgggta atgcgcccac aagttctgct
    24421 taatcgattg aaaaaacatc ttttttttaa agatgtgttc gatgctgtgt acctgtgctc
    24481 acagatgtct actttttcgc gaaaacgtag atctctaccg cccaacgaaa agcatgaaag
    24541 cgatcacgaa tcccattggg tcgtcatgtt ctgccgatcg catcttccta tcctcgctcc
    24601 aggcctgccg cataaccaat caggcttcct acttacagaa ttgagaaaag aggatgtgga
    24661 aatgctcagt caaattcaac gctttggcgg cgcgatgttc acgccagtgc tgctgtttcc
    24721 cttcgccggg attgtggtgg gtcttgccat cttgctgcaa aacccgatgt ttgtcgggga
    24781 atcactgacc gatccgaaca gtttattcgc gcaaatcgta cacattattg aagagggcgg
    24841 ttggacggta ttccgtaata tgccgctgat ttttgctgtc ggtttaccca ttggccttgc
    24901 taagcaagcg caggggcgtg cttgtctggc ggtgatggtg agtttcctga cctggaacta
    24961 tttcatcaac gcgatgggaa tgacctgggg aagctacttc ggcgtcgatt tcactcagga
    25021 cgcggtggca ggtagcggtc tgacaatgat ggccgggatt aaaaccctcg ataccagcat
    25081 tatcggcgca attatcattt ccggcattgt gacggcgctg cataaccgtc tgttcgataa
    25141 aaaactgccg gtttttctcg gcattttcca ggggacgtct tatgtggtga ttatcgcctt
    25201 cctggtgatg atcccctgtg cctggctgac attgctcggc tggccaaaag tacaaatggg
    25261 gattgaatct ctgcaagcgt tcctgcgttc ggcgggtgca cttggggtct gggtttacac
    25321 cttcctcgaa cgtattctga tcccaaccgg tttacaccac ttcatctacg gaccgtttat
    25381 ctttggtctg gcagctgttg aaggcggcat tcagatgtac tgggcgcagc atctgcaaga
    25441 gttcagtctg agcgccgagc cgctgaaatc gttgttcccg gaaggcggtt ttgccctgca
    25501 cggtaactca aaaatctttg gtgccgtggg catttcttta gcgatgtact tcactgccgc
    25561 accggaaaat cgggtaaaag tggcgggctt gctgattccc gcaaccttaa ccgccatgct
    25621 ggtgggaatt accgaaccgc tggaatttac cttcctgttc atttcaccgt tgctgtttgc
    25681 ggtacacgcc gtgctggcgg cctcaatgtc gaccgtaatg tatctctttg gtgtggtggg
    25741 caacatgggc ggaggtctga ttgaccaggt tttaccgcaa aactggatcc cgatgttcag
    25801 caaccacgcg gatatgatgc tgacccaaat cgccattggg ttgtgcttta ccctgctgta
    25861 cttcgtggtt ttccgcaccc tgattctgca gttcaacatg tgcacgccgg gacgtgaaga
    25921 tgcggaagtg aaactctact caaaagccga atacaaagcc tcgcgaggcc aaaccaccgc
    25981 ggcagagcca aaaaaagagc tggatcaggc tgccggtatc ctgcaagccc tgggcggggt
    26041 cggcaatatc tccagcatta acaattgtgc gacgcgtcta cgcattgcac tgcatgacat
    26101 gtcacaaacg ctggatgacg aagtctttaa aaagctggga gcgcacggcg tcttccgtag
    26161 tggcgatgcc attcaggtga tcattggtct gcatgtatcc cagctgcgtg aacagctcga
    26221 tagcttaatt aattctcatc aatcagcaga aaatgttgcc attacggagg cagtataatg
    26281 accaaattct cagtggttgt cgcaggcggt ggaagcacct ttacgccagg catcgtgttg
    26341 atgctcctgg cgaatcagga gcgtttcccg cttcgtgcac tgaaatttta tgataacgat
    26401 ggcgcgcggc aggaagtgat tgccgaagcc tgtaaagtca tccttaaaga gaaagcgccg
    26461 gacattgcgt ttagttacac caccgatcct gaagtggcgt tcagcgacgt tgattttgtc
    26521 atggcgcaca tccgcgtcgg caagtacccg atgcgcgaac tggatgaaaa aatcccactg
    26581 cgccacggtg ttgttggtca ggaaacttgc ggacccggcg gaatagcgta cggcatgcgt
    26641 tccattggcg gcgtcctgga actggtggat tatatggaaa aatattcacc aaatgcctgg
    26701 atgctcaact actccaaccc ggcagccatt gtcgcagaag ccacgcgtcg tctgcgcccg
    26761 aatgcgaaaa tcctcaacat ctgtgacatg ccaatcggta ttgaaagccg gatggcgcaa
    26821 attgttgggc tgcaagatcg caaacagatg cgcgtgcgct actacggcct gaaccactgg
    26881 tggtcggcga tatcccgcag tttcagaaag ggttaatgag tcagcaagtg gcggtggaaa
    26941 aactggtcgt ggacgcctgg gaacagcgtt catatcagca cctgtggcag gcgattacgt
    27001 tgtcgaaaac ggtaccgagc gcctcggtcg ccaaagctat tctggatgag ttgctggagg
    27061 ccaacaaagc gtactggcca gagttacgtt aattgtgtgg actggcatcc gttgggtgct
    27121 ggtccgtatt ctttagcacg gctgacggcg cataaccaaa tttatgttta aaggctttgc
    27181 tgaaatgaaa ggagtcgaag aagttgagcg tatcagcgac ttcgccaacc gagtttcctt
    27241 gttgctttaa tagcgatagc gccagatcca gacgcgcatc gaggtaatat tctttcggcg
    27301 tcttgccggt atagcgtaaa aacagacgac gcaaccaggc ttcgctacag gggatcgtgg
    27361 cagccatatc agctacgctc cagcgttgtt gcagactggc atgtaaagtg gcaattaatt
    27421 tttcaatttg ccgccgttgt ggatcttttt taccatctgc gtacataaga caaatccact
    27481 gataaataat tttcgtcaga aaagcgactg ccagattatt ttttattgct tctggtgaag
    27541 ttattaactc agcaacttcg gtgagttcct gattataaat ttcgccatta taaataacgc
    27601 tttgctgacc aacgggaata tccatcatac tggtgggggt aaattccatc cagtactgtt
    27661 cccagactaa accttcacag tgataagagt gaatatccat tggctttaag aatataatac
    27721 agttgccatg cagagttatt tgttcgccat tctttaaaag aattttccca catccttgta
    27781 cggtatacac cgcaacccat gagttggcga taagcggttt cttcttgtcc cgcggccaga
    27841 tcacccggta atgctcgtcg gcgtaggtat gccagagcga gatcagagaa attccgctgg
    27901 agatcacatt ttcgttaatc aataagggaa tattgtacgg agtttcgttt tttacatctg
    27961 aactttgcaa ttttccattc attttaatat ccctccggcg aattatgctc acaacctgaa
    28021 cttaacagaa aacaatccgt tcagggatac gacgtttgta ttttaagaat ctgactgcct
    28081 gacccgacgc attttacctc tccctatatt catgcgtccg ggacataaac ataaataagg
    28141 gctatgagat gaattcgtta caaatcttga gttttgtcgg ttttacgctg ctggtggcgg
    28201 tgatcacctg gtggaaggtc cgcaaaacag ataccggatc gcaacaaggc tattttcttg
    28261 ccggacgttc actaaaagcg ccggttattg ccgcttcgtt aatgctaacc aacctttcca
    28321 cggaacaact ggtcggcctt tccgggcagg cctacaaaag cggcatgtcg gtgatgggct
    28381 gggaagtgac ttcagcggtg acgctgatct tcctcgcgct aatcttttta ccgcgctatc
    28441 tgaagcgcgg cattgccacc atccccgatt ttctggagga acgttatgat aaaacgacgc
    28501 gtattatcat cgacttctgc ttcctcattg ccaccggcgt ctgctttctg ccgattgttc
    28561 tctactccgg cgcgttggcg ctcaacagcc tgtttcacgt cggggaatcg ctacagattt
    28621 ctcacggtgc ggctatctgg ctactggtaa ttttgcttgg tctggcggga attttgtatg
    28681 cggtgatcgg cggactgcgc gcaatggcag tggcggactc catcaacggt attgggctgg
    28741 ttatcggcgg gttgatggtg ccggtatttg gcctgatcgc gatgggcaag ggcagcttta
    28801 tgcagggcat tgagcaactc accaccgttc acgccgagaa attaaactca atcggtggcc
    28861 cgaccgatcc cttgccgatt ggcgcggcat ttaccggttt gattctggtg aacacctttt
    28921 actggtgtac aaatcagggc atcgtgcaac gcacgctggc gtcaaaaagc ctggcggaag
    28981 ggcaaaaggg ggcgctgtta acggcggtgc tgaaaatgct cgacccgctg gtactggtgc
    29041 tgccagggtt gattgcgttt catctgtatc aggatttacc gaaagccgac atggcctacc
    29101 cgacgctggt caataacgtt ctgccagtgc caatggtggg tttcttcgac gcggtgttat
    29161 ttggtgcggt gatcagtacc ttcaacggct ttctgaatag cgccagtacg ttattcagta
    29221 tgggtattta ccgtcgcatc attaaccaga atgccgagcc gcagcagctg gtcaccgtcg
    29281 ggcgcaaatt tggtttcttt atcgctatcg tttcggtgct ggtcgcgccg tggatcgcca
    29341 acgcgccgca ggggctgtat agctggatga aacagctcaa cggcatttac aacgtgccgc
    29401 tggttaccat catcattatg ggctttttct tcccgcgcat cccggcgctg gcggcaaaag
    29461 tggcgatggg gattggcata atcagctaca tcaccatcaa ctatctggtg aagttcgact
    29521 tccatttcct ctatgtgctg gcctgtacgt tctgcatcaa cgtggtcgtg atgctggtga
    29581 tcggttttat caaaccgcgc gccacgccgt tcaccttcaa agatgcgttt gcggtggaca
    29641 tgaaaccgtg gagaaacgtc aagatcgcgt caattggcat cctgttcgcg atgattggcg
    29701 tctatgccgg gctggctgaa ttcggcggct acggtacgcg ctggttagcg atgatcagtt
    29761 atttcattgc cgccgtagtg attgtctacc tgatttttga cagctggcgg catcgtcacg
    29821 acccagccgt aacctttact cccgacgcga aggatagcct atgaaacgcc ccaattttct
    29881 gttcatcatg accgataccc aggccaccaa tatggtcggt tgctatagcg gtaaaccgct
    29941 gaatacgcaa aatattgata gtctggcggc ggaaggtatt cgctttaatt ccgcctacac
    30001 ctgttcaccg gtttgtacgc ctgcacgcgc cggactattt accggtatct acgctaacca
    30061 gtccggcccg tggaccaaca acgtcgcgcc aggcaaaaac atctccacta tggggcgcta
    30121 ctttaaggat gccggctatc acacctgtta catcggcaaa tggcatctcg acggtcatga
    30181 ctatttcggc actggcgagt gtccgccgga gtgggacgct gattactggt tcgatggggc
    30241 gaactacctt agcgaactga cggaaaaaga gatcagcctg tggcgcaatg gcctaaacag
    30301 cgttgaggat ttacaggcga accatatcga cgaaaccttc acctgggcgc accgcatcag
    30361 caatcgggcg gtagattttc tgcaacagcc cgcgcgcgcc gaggaaccct tcctgatggt
    30421 ggtttcgtat gatgagccgc atcacccgtt cacctgtccg gtggagtatt tagagaaata
    30481 cgctgatttt tactacgatc tgggcgagaa agctcaggat gacctggcga acaaaccgga
    30541 acatcaccgc ttatgggcgc aggcgatgcc atcgccagtc ggtgatgacg ggctttatca
    30601 ccatccgctc tattttgcct gtaatgactt tgttgatgac caaatcggac gggtcatcaa
    30661 tgccttaacg ccagagcaac gtgaaaatac gtgggtcatt tatacttccg atcacggcga
    30721 aatgatgggc gcacataagc tgatcagtaa aggagcggcg atgtatgacg acatcacccg
    30781 tattccgctg atcatccgtt cgccgcaagg ggagcggcga caggtcgata cgccagtcag
    30841 tcatatcgat ttactgccga caatgatggc gctggcagat attgaaaaac cagagattct
    30901 gccgggggaa aatatccttg ccgtgaaaga gccacgcggt gtaatggtgg aatttaaccg
    30961 ctacgagatt gagcatgaca gctttggcgg ttttattccg gtgcgttgct gggtgacgga
    31021 tgactttaaa ctggtactca acctcttcac cagtgatgaa ctttacgatc gccgtaatga
    31081 cccaaatgaa atgcataacc tgatcgatga tatccgtttt gcagacgttc gcagcaaaat
    31141 gcatgacgcc ttattggatt acatggacaa aattcgcgat ccgttccgca gttaccaatg
    31201 gagcctgcgt ccgtggcgta aagatgcact gccgcgctgg atgggggcat ttcgtccacg
    31261 cccacaagat ggctattcgc cggtggtacg tgactatgac accggcctac cgacgcaagg
    31321 agtgaaagtg gaagagaaaa aacagaagtt ctgacgataa tgcccgctga tgaacacccg
    31381 gcgggcatta ttcaggctgc ttaaaccagt gaaccgccgg tcaggaagga aaaaatcagg
    31441 aatatcagca aaatgctgca cacgatgccg ataccaaaaa gcaaggtatg gaaagccagc
    31501 gtcccgccgc caaaaaccag cgcgccgcga atgccccagc gattttcctt acgcataccg
    31561 ccaataaatg cgcaaagtag cgcagcaatg gccagtgcga taccgctttg atcgaggatt
    31621 ttatcgacat ctacgtcctc ttttttctcc acggtggtaa ttttttcgcc ttttattccg
    31681 gcgagaagac ctcttttaat ttcagccgtt ttgtccgcca cgatgctttc aaacgttggc
    31741 ggtggtgcgg aaaaagggcc gaatgaaaag tggataatcc ccatcattaa agcgatggca
    31801 ccgaacagca tgcccaatga actgatttta ttttgagcta tgatacccat ctgacctcct
    31861 tgtagcggat ggcgaaagca tatcaggaaa cgggcggacg aaattcttaa aaaaaaaccg
    31921 ctaaaggtag agcgttagtg aaattgcaaa ggagcaacaa tgaaaatttc ccgccttgga
    31981 gaagcgcccg attaccgctt ctcgctggct aatgagcgta cttttctggc gtggatccgt
    32041 accgcactag gatttctggc ggcgggggtt gggcttgatc aacttgcgcc agatttcgcc
    32101 acgcccgtca ttcgtgaact gctggcgttg ttgttgtgtc tgttttccgg cgggttagcg
    32161 atgtatggct acctgcgctg gttacgcaat gaaaaagcga tgcgtctgaa agaggacctg
    32221 ccctacacca acagcttatt aatcattagc ttaatattga tggtcgtggc ggtgatcgtt
    32281 atgggactgg tgttgtatgc cggatagccg caaagccaga cgcattgccg acccaggact
    32341 gcagccggag cgcacatcac tggcgtggtt tcgtaccatg ctgggctacg gcgcgttgat
    32401 ggcgttggct atcaaacaca actggcacca ggcgggcatg ttattctgga tttcgattgg
    32461 cattctcgcc atcgtggcgc tgatcctctg gcactacacc cgtaatcgca atttaatgga
    32521 tgtcacgaat agcgattttt cccaatttca cgtagtccgt gacaaatttt tgatctccct
    32581 cgcggtgtta tctctcgcaa tactgtttgc tgtaacgcat atacatcaac ttatcgtatt
    32641 tattgagaga gtcgcatgac aggaagtcag gtcattgatg ctgaagagga caggcataaa
    32701 ctggttgtag agtacaagga tgctctgcaa cctgccgatt tttatcacaa tttcaaacag
    32761 cggggcatcc gctccgtaca gctcattccc tatcttgaat ttgatgaccg gggcgatctg
    32821 acggccgctt cggtgacagc agagctgtgg gggaaatttt taattgccct gttcgagtgt
    32881 tgggtgcggg cagatatcag tcgtatctcg attgagcttt ttgacgccac cttacaaaaa
    32941 tggtgtggca gcgaaaaccc gcaaccgcgc cgtgactgcc aggcgtgcga ctggcatcgc
    33001 ctgtgcccgc acgctcgcca ggagacgcca gacagcgtgc tttgtgcggg ctatcaggcg
    33061 ttttattctt actccgcacc gcatatgcgg gtgatgcgtg atctgataaa gcagcatcgt
    33121 tctcccatgg agctgatgac gatgctgcgg tgacgtgcgc ttaaacgggc tgcccctgat
    33181 gcgacatccg cgtcgccagc ggcagccagc acagcacgat caacaatccc attaaggtca
    33241 tcaacaaccc caggctgccc tgaccggttt gcggcaacat cgcagagagc gacgccagca
    33301 cgccggaacc aatgttttgc agaccgccga ccagcgcgcc agccgtgccc gccaggaagg
    33361 ggaacggctc catcgcgccg ctggtcgcca gcggaaacag catcccggca ccgaaaaaga
    33421 acagcgcggc gggaacgagc agcgtccaga cattcatcac gccaaaccag tcggggatcc
    33481 acatcagcaa gccagccagc aggcagcaga taaccgactg ccacattaac gtggagaagc
    33541 gtttattggg acgtccggca aaccatgcgc caaaaaacgc tgccggaatc ggcagaataa
    33601 acaaaatact gaccgtcata ctgctcagcc ctaacaccgc gcccatcagc acgccggagc
    33661 aggcttcaaa ggcggcaatc ccggccagac cgccaatcag catcagcaaa taacagttaa
    33721 aaccgctgtt accgaaaagc gttttataac tggtaagcag gcgcgtgcgc ggtgcatcga
    33781 ccggacgcgt ttccggcatc cagcgggcca tactgaaggt cacaccagca caaagaacca
    33841 acaaaaagag ataacaggcg cgccagttcc acatcgtatc cagcagaccg ccgattagcg
    33901 gtgcgagcaa cggactgacg agaatcccca tgtttaacag gctgttagca tggcgcaact
    33961 gtgtccgttc atataaatct cgcggcaaag tacgcgccat tacgccgcca acgccggtgc
    34021 ccatcccctg catcgcgctg gcggcaatca acaccgtcaa actggaggtc gtgaccgcga
    34081 ccagcgttgc cagcataaaa atggacattc cgacgaggat caccggtcgg cggcccacgc
    34141 ggtcggaaat cgggccataa aacagctgtg agacaccgta agtcagcaga taagcgccca
    34201 ttacgctctg caccgcccct tcacggacgt tgagatcgcg cgccatatcg gcaatagctg
    34261 gaatataaat ggtttgcgcc atctgaccga cggccacgag taataccaac atcaataaca
    34321 aattgacgtt tctttgcctt ttcattatca cggatgcttt tataaaaata attagaataa
    34381 gtcactacgc attcccataa atgcgggaga ggaatctatc acattgatga atgttagcca
    34441 gatatacgcc aggaatgggg aattgtttag cggcagaata tgtaaacaaa agcggcaata
    34501 aatgttgccg ggagggatta cgtgcgaact gtaaccctct ccccaaaggg gcgaggggga
    34561 aaccaccgtt ccctttcaga aaatgagcta aacattgcac gggacggtcc cctcgcccct
    34621 ttggggagag ggctagggtg aggggcacag gcagcgcagg tgcaaacatt ctgctggtag
    34681 aagaagcccc caactccaac ccgcacgcta aatataaaag gggagcggtt acccgctccc
    34741 ctttggtgcg acttgaatct gaattacttc aggtatttca gaacagcatc aagcagttgc
    34801 agtgctgcaa caatgagttt gaggataaga atggcgatat ccaccaggct catacgcgtc
    34861 tcctgtggtt caggagcact gccagctgac gtacctttcc gctgcctgtt gccagctgtt
    34921 gtgcacgcgg tatcttaagg agaaacgttg tgcttgttga cataacacag tgtgctcacg
    34981 cggggttaag gcgctgacgg caccacccgt ttcagccagg acttagtgcg ccgggtaacg
    35041 atgcggcccc cgcataacac attgcgtaca gtgatattat agaagtttac tgtataaata
    35101 aacagtaata tttggacaaa acgcaaactg tgatcgatac tcaactatgc cctaaatacg
    35161 tcaaaattcg tgccgaaatt gcgcgttctg cgcggaacac gtatactttc agtgttgaca
    35221 taatacagtg tgctttgcgg ttaccagccg caggcggctg atgaaacctc gctccggcgg
    35281 ggttttttgt tatctgcaat tcagtacaaa acgtgatcag cccctcaatt ttcccttgct
    35341 gaaaaatttt ccattgtctc ccctgtaaag ctgtgcttgt ataaatattg ttaaacacaa
    35401 aaccaacaag gtccccaatg actacttcca tgttcaacgc aaaactacta ccaactgcgc
    35461 tatccgccgc agtggtcgtc gtgcgtgtgg tggtggtcgt cggcaatgcg ccgtagggac
    35521 tggaacaaca cacgattcca aaaccccgcc ggcgcaaacc gggcggggtt tttcgtttaa
    35581 gcacctcccg gaaagtcggc ccagaagaaa aggactggag catggcaagt tcgggcacaa
    35641 catcgacgcg taagcgcttt accggcgcag aatttatcgt tcatttcctg gaacagcagg
    35701 gcattaagat tgtgacgggc attccgggcg gttctatcct gcctgtttac gatgccttaa
    35761 gccaaagcac gcaaatccgc catattctgg cccgtcatga acagggcgcg ggatttatcg
    35821 ctcaggggat ggcgcgcacc gacggtaaac cggcggtctg tatggcctgt agcggaccgg
    35881 gtgcgactaa cctggtgacc gccattgccg atgcgcggct ggactccatc ccgctgattt
    35941 gcatcaccgg tcaggttccc gcctcgatga tcggcaccga cgccttccag gaagtggaca
    36001 cctacggcat ctctatcccc atcaccaaac acaactatct ggtcagacat atcgaagaac
    36061 tcccgcaggt catgagcgat gccttccgca ttgcgcaatc aggccgccca ggcccggtgt
    36121 ggatagacat tcctaaggat gtgcaaacgg cagtttttga gattgaaaca cagcccgcta
    36181 tggcagaaaa agccgccgcc cccgccttta gcgaagaaag cattcgtgac gcagcggcga
    36241 tgattaacgc tgccaaacgc ccggtgcttt atctgggcgg cggtgtgatc aatgcgcccg
    36301 cacgggtgcg tgaactggcg gagaaagcgc aactgcctac caccatgact ttaatggcgc
    36361 tgggcatgtt gccaaaagcg catccgttgt cgctgggtat gctggggatg cacggcgtgc
    36421 gcagcaccaa ctatattttg caggaggcgg atttgttgat agtgctcggt gcgcgttttg
    36481 atgaccgggc gattggcaaa accgagcagt tctgtccgaa tgccaaaatt attcatgtcg
    36541 atatcgatcg cgcagagctg ggtaaaatca agcagccgca tgtggcgatt caggcggatg
    36601 ttgatgacgt gctggcgcaa ttgatcccgc aggtggaagc gcaaccgcgt gcagagtggc
    36661 accagttggt agcggatttg cagcgcgaat tcccgtgtcc aatcccgaaa gcgtgcgatc
    36721 cattaagcca ttacggcctg atcaacgccg ttgccgcctg tgtcgatgac aatgcgatta
    36781 tcaccaccga tgtgggtcag catcagatgt ggaccgcgca agcttatccg ctcaatcgcc
    36841 cacgccagtg gctgacctcc ggtgggctgg gcacgatggg ttttggcctg cctgcggcga
    36901 ttggcgcggc gctggcgaac ccggatcgca aagtgttgtg tttctccggc gacggcagcc
    36961 tgatgatgaa tattcaggag atggcgaccg ccagtgaaaa tcagctggat gtcaaaatca
    37021 ttctgatgaa caacgaagcg ctggggctgg tgcatcagca acagagtctg ttctacgagc
    37081 aaggcgtttt tgccgccacc tatccgggaa aaatcaactt tatgcagatt gccgccggat
    37141 tcggcctcga aacctgtgat ttgaataacg aagccgatcc gcaggctgca ttgcaggaaa
    37201 tcatcaatcg ccctggcccg gcgctgatcc atgtgcgcat tgatgccgaa gaaaaagttt
    37261 acccgatggt gccgccaggt gcggcgaata ctgaaatggt gggggaataa gccatgcaaa
    37321 acacaactca tgacaacgtg attctggagc tcaccgttcg caaccatccg ggcgtaatga
    37381 cccacgtttg tggccttttt gcccgccgcg cttttaacgt tgaaggcatt ctttgtctgc
    37441 cgattcagga cagcgacaaa agccatatct ggctactggt caatgacgac cagcgtctgg
    37501 agcagatgat aagccaaatc gataagctgg aagatgtggt gaaagtgcag cgtaatcagt
    37561 ccgatccgac tatgtttaac aagattgcgg tgttttttca gtaaccgctc aaggcttgaa
    37621 caacatcgcg cttatcgtta aggtaagcgc gttttttttt acccgccagg acaagaccat
    37681 gatcaccgtt gcccttatag acgatcacct catcgtccgc tccggctttg cgcagctgct
    37741 ggggctggaa cctgatttgc aggtagttgc cgagtttggt tcggggcgcg aggcgctggc
    37801 ggggctgccg gggcgcggtg tgcaggtgtg tatttgcgat atctccatgc ccgatatctc
    37861 cggtctggaa ctgctaagcc agctgccgaa aggtatggcg acaataatgc tctctgttca
    37921 tgacagtccg gcgctggttg agcaggcgct taacgcgggg gcacgcggct ttctctccaa
    37981 acgctgtagc cctgacgaac tgattgcggc ggtgcatacg gttgccacgg gcggctgtta
    38041 tctgacgccg gatattgcca ttaaactggc atccggtcgt caggacccgc taaccaaacg
    38101 cgaacggcag gtggcggaaa aactggcgca aggaatggcg gtgaaagaga ttgctgccga
    38161 actgggcttg tcgccgaaaa cggtgcacgt ccatcgcgcc aacctgatgg aaaaactggg
    38221 tgtcagtaac gacgtagagc tggcgcgccg catgtttgat ggctggtgat gaagacgttg
    38281 ttctcccgct taattaccgt tattgcctgc ttttttatct tctctgccgc atggttttgc
    38341 ctgtggagta tcagcctgca cctggttgag cgccctgata tggcggtgct gttatttccg
    38401 tttggtctgc gtctggggct aatgctgcaa tgcccgcgcg gatactggcc ggtgctgctg
    38461 ggcgcggagt ggctgctgat ttactggcta acgcaggcgg tcggtttaac ccattttccg
    38521 ttattgatga tcggtagttt actgacgtta ctgcccgtgg cgctgatctc gcgctatcgc
    38581 catcagcgtg actggcgcac cttgctgtta cagggtgcgg cgttaacggc ggcggcgttg
    38641 ttgcagtcgc tgccctggct ttggcacggc aaagagtcgt ggaatgcgct gttgctgact
    38701 ttaactggcg gcctgacgct ggccccgata tgtctggtgt tctggcacta tctcgccaat
    38761 aacacctggc tgccgctcgg cccgtcactg gtttctcagc cgatcaactg gcgcgggcga
    38821 catctggtct ggtacttgct gctgtttgtt atcagtctct ggctccagtt gggattgccg
    38881 gacgaactgt cgcgctttac gccattttgt ctggcgctgc cgattatcgc gctggcctgg
    38941 cactacggtt ggcaaggggc gctgatcgcg acgttgatga acgccatcgc gctgatcgcc
    39001 agtcaaacct ggcgcgatca tccggtggat ttattgctct cgttgctggt gcaaagtctg
    39061 acagggttgt tactgggcgc tggcatccag cggttgcgtg aacttaacca gtcgctgcaa
    39121 aaggaactgg cgcgcaatca gcatctggct gaacggttgc tggaaaccga agagagcgtg
    39181 cgtcgtgatg tggcgcgtga gctgcatgat gatatcggtc agaccatcac tgctattcgt
    39241 actcaggcgg gcattgttca gcggctggcg gcagataacg ccagcgtgaa gcagagcggg
    39301 cagctcatcg aacaactatc gctgggcgtt tacgacgcgg tgcgccgttt gttgggtcgg
    39361 ttacgtccgc gccagttgga tgatctcacc ctggagcagg ccatccgctc actgatgcgg
    39421 gaaatggagc tggaagggcg cggtattgtc agccatctcg aatggcgaat cgatgaatca
    39481 gcattaagtg aaaaccagcg cgtgacgctg tttcgtgtct gccaggaagg gctgaacaac
    39541 attgtgaaac atgctgatgc cagcgcggtc acgctgcaag gctggcagca ggatgaacgg
    39601 ttgatgctgg ttattgaaga cgatggcagc ggtttgccgc cgggttccgg gcaacaaggt
    39661 tttggcctca ccggaatgcg cgagcgcgta acggcgctgg gtggcacatt acacatttcc
    39721 tgtctgcacg gcacgcgtgt cagcgtttct ctacctcaac gctatgtcta aggtttgatg
    39781 atgttgccgt ttctgaaagc gcctgccgat gcgccattaa tgactgataa atatgaaatt
    39841 gatgcccgct atcgctactg gcgtcggcat attctgctga ccatctggct gggttatgcg
    39901 ctgttttact tcacgcggaa aagttttaac gccgccgtac cagaaatcct tgctaacggc
    39961 gtgctcagcc gtagcgatat cggcctgtta gcgaccctgt tttacattac ctatggcgtg
    40021 tcgaagtttg tctccggcat tgtcagcgat cgctcaaatg cccgttattt tatggggata
    40081 gggcttatcg ccacgggcat tatcaacatt ctgtttggct tctcgacgtc gctatgggcg
    40141 tttgccgtac tctgggtgct gaacgccttt ttccagggct ggggttcacc ggtgtgtgcg
    40201 cgtctgttaa ccgcctggta ttctcgtacc gagcgcggcg gttggtgggc attatggaac
    40261 acggcgcata acgtcggcgg cgcactcatt cccattgtga tggcagcggc tgcactacat
    40321 tacggctggc gtgccgggat gatgattgct ggttgtatgg cgatcgtcgt gggaattttt
    40381 ctctgctggc ggctgcgcga tcgcccgcag gcgttaggtt taccggcggt cggtgaatgg
    40441 cgacacgacg cgctggagat agctcaacaa caagaagggg cagggctgac gcgtaaagag
    40501 atcctcacca aatatgtgtt gctgaatccg tatatctggc tgctttcgtt ttgctatgtg
    40561 ctggtctatg tggtccgggc ggcgatcaac gactggggca atttgtatat gtccgagacg
    40621 ctgggcgtcg atctggtcac ggcgaatacg gcagtgacga tgtttgaact gggcggattt
    40681 atcggtgcgc tggtagccgg ttggggctcg gacaaattgt ttaacggcaa ccgagggccg
    40741 atgaatttga ttttcgccgc cggaattttg ctttcagtcg gctccctgtg gctgatgcca
    40801 tttgccagct acgtgatgca ggcaacctgc ttcttcacca ttggtttttt tgtctttggc
    40861 ccacagatgt taatcggtat ggcggcggca gagtgttccc acaaagaggc ggcaggggcg
    40921 gcgacggggt ttgtcggctt gtttgcttat ctgggggcgt cgcttgctgg ttggccgctg
    40981 gcgaaagtac tcgatacctg gcactggagc ggattttttg tggttatctc tatcgccgcc
    41041 gggatttccg cactgctgtt actgcccttt ttgaacgccc agacaccgcg cgaagcgtga
    41101 tgcatctcac cttttcactt catatccggc aaaactaaga aattttccag gttttgcctg
    41161 gacgctatct caggcctgat ttgctgctga tttttacaat gcatgcctca cgcaggtatt
    41221 catttcagga gtaacccatg ctggctttct taaaccaggt tcgcaagccg accctggacc
    41281 ttccgctcga agtgcggcgc aaaatgtggt tcaaaccgtt catgcaatcc tacctggtgg
    41341 tctttatcgg ctacctgacg atgtacctga ttcgcaagaa ctttaacatc gcgcagaacg
    41401 acatgatctc gacctacggg ttgagcatga cgcagctggg gatgatcggc ctgggtttct
    41461 ccatcactta tggcgtgggt aaaacgctgg tttcctacta cgccgacggc aaaaacacca
    41521 aacaattcct gccgttcatg ctgatcctgt ctgccatttg tatgctgggc ttcagtgcca
    41581 gtatgggcag cggctcggtt agcctgttcc tgatgattgc cttctacgcc ttaagcggct
    41641 ttttccagag taccggcggt tcgtgcagtt actccaccat caccaaatgg acgccgcgtc
    41701 gtaaacgcgg gacattcctc ggtttctgga atatttctca caaccttggc ggtgcaggcg
    41761 cagcaggtgt ggcgctgttc ggggcaaatt acctgttcga tggccatgtc atcggcatgt
    41821 ttatcttccc gtcgattatc gcgctgattg tcggttttat cggcctgcgt tacggcagcg
    41881 actccccgga atcttatggc ctcggcaaag ctgaagaact gttcggcgag gagatcagcg
    41941 aagaggacaa agagacagaa tctaccgata tgaccaagtg gcagatcttt gttgagtatg
    42001 tgctgaaaaa caaagtgatc tggctgctgt gcttcgccaa cattttcctc tatgtggtac
    42061 gtattggtat cgaccagtgg tcaaccgtat acgcgttcca ggaactgaaa ctctctaaag
    42121 cggtggcgat tcagggcttt acgctgtttg aagctggtgc gctggtcggt acgctgctgt
    42181 ggggctggct ctctgacctg gcgaacggtc gccgtggcct ggtggcctgc atcgcgctgg
    42241 cgctgattat cgccacgctc ggtgtgtatc aacatgccag caacgaatat atctatctgg
    42301 cttctctctt tgcgttgggt ttcctggtct ttggcccgca attgttgatt ggtgtggctg
    42361 ctgttggctt tgtacctaaa aaagcgattg gcgctgccga tggtattaaa ggcacctttg
    42421 cttacctgat tggtgacagc tttgccaagt taggtctggg aatgattgcc gatgggacgc
    42481 cggtattcgg ccttaccggc tgggcaggca ccttcgccgc acttgatatc gccgcgattg
    42541 gttgtatctg cctgatggcg atagtggcgg tgatggaaga acgcaaaatc cgccgcgaga
    42601 aaaaaattca gcagttgaca gtggcataaa cgtaactggt gacttttgcc cggcatgacg
    42661 ccgggctttt ttattattcc gtgacttcca gcgtagtgaa ggcaaacttc tcgccatcaa
    42721 atagcccctg actggttagt tttagcgcgg ggatcactgg cagagaaaga aacgccatct
    42781 gaataaacgg ctcatcgggt aacggaccgc attcacgggc ggcggctttc aaggcgtcaa
    42841 tttgttccgc cagcgactgc gccgtgtcgg tgctcatcag gccggcaatg ggcaacggca
    42901 gatgactttg gacctggccg ttacgtacca cgcacagccc gccgccatcc tgaatcacct
    42961 gattgaccgc cagcgccatc tcttcggcac tgcgaccgat caccacaata ttatggctgt
    43021 catggctgac cgtcgcagcc agcgcacctt cattcagtcc aaagccgcca agcaaaccac
    43081 aagccggagc cagccgttgc ccgtaacgct caagtacggc aataaagctc acatcatcgc
    43141 gatcaaaacc attttcgctg tagacgctgg agtgggagtg cgtaatcaat tcgttatgga
    43201 tgacgtcaat gacccgatag cgttttccgg gcgtaaattg cagggcaaag tcgctggcgg
    43261 aaactggctg gcgggcaatg gtgttgccat atggcggagc ggattgtgcc agtctcgccg
    43321 actcttccgc ctgtaaggtt tgcgcatcaa tcggctcgcc tttcaccagt acctgctgca
    43381 ccgtgacctt acgcgcatcg ctcaacagga cgatatcggc ctgcttgccg ggtgccagta
    43441 agccgaggtg attcagacca aagtggcgcg ccgtcgacca gctggcgacg cgatatgcca
    43501 catgcagcgg cacattgtgt tgttcgatca ggcggcgaat taaggcatcg atgtgtcctt
    43561 catgggcgat ctcccacggg ttacggtcat cggtacagag catgcattgc gggctgttaa
    43621 attcgttgat caacggtgcc agcgcgttga gattgcgggc agcggaccct tcgcggatca
    43681 tcaacgacat gccgagttgt aatttccggc gtccttcttc cagctgataa ctttcgtggc
    43741 agttttcaat acccgcagta atataggcgt taagttcttt accacccaaa cccgggcagt
    43801 gaccgtccag cgtcaggtgg cgaaatgcat ccagtttatc gagcagcgca ttctgcccgc
    43861 taattacgcc agggtagtcc atcatttctg caaggccggt aacctgcgga tggtcccgcc
    43921 aggcgagcat ctgttcaagg gtaaaactgg caccgttaac atcgcagcct tccagggcgg
    43981 gtacgcaaga gctgacctgt aagtactggt tttgccttgc ctgttcggca cagcgggcaa
    44041 accaggcgaa tccggcttct cccatcacgt tgacgatttc atgagggtcg caaataacgg
    44101 tcgtcaggcc gcgcggcagg gtagcggttt caaaagtgac cggcgtcatc atgctggatt
    44161 caatatgcag gtgagcatca ataaaccctg gcaccgccgt tgcgccgcga gcatcaatcc
    44221 gctgcaaagc cggagcatca gcgtattctg cgccaacacc ggcaatgtaa cgtcctttaa
    44281 tcacaattgg gccggaaatt tctccgccat tgatcaggtc gagaatagag acattatcaa
    44341 taatataatc ggcaacagcg tcgccacggg aaacggc
//
LOCUS       DAPPNJ010000008        44034 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000008 DAPPNJ010000000
VERSION     DAPPNJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.008"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..198)
                     /locus_tag="RPM73_000332"
     CDS             complement(<1..198)
                     /locus_tag="RPM73_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDX6518728.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGI"
     gene            complement(201..920)
                     /gene="sanA"
                     /locus_tag="RPM73_000333"
     CDS             complement(201..920)
                     /gene="sanA"
                     /locus_tag="RPM73_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDX6518729.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1070..1954)
                     /gene="cdd"
                     /locus_tag="RPM73_000334"
     CDS             complement(1070..1954)
                     /gene="cdd"
                     /locus_tag="RPM73_000334"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDX6518730.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAENADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2084..2779)
                     /locus_tag="RPM73_000335"
     CDS             complement(2084..2779)
                     /locus_tag="RPM73_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDX6518731.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2776..3174)
                     /locus_tag="RPM73_000336"
     CDS             complement(2776..3174)
                     /locus_tag="RPM73_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDX6518732.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3413..4363
                     /gene="dusC"
                     /locus_tag="RPM73_000337"
     CDS             3413..4363
                     /gene="dusC"
                     /locus_tag="RPM73_000337"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDX6518733.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(4736..5431)
                     /gene="aqpZ"
                     /locus_tag="RPM73_000338"
     CDS             complement(4736..5431)
                     /gene="aqpZ"
                     /locus_tag="RPM73_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDX6518734.1"
                     /translation="MFRKLTAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            5857..7515
                     /locus_tag="RPM73_000339"
     CDS             5857..7515
                     /locus_tag="RPM73_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDX6518735.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEECWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(7512..8468)
                     /locus_tag="RPM73_000340"
     CDS             complement(7512..8468)
                     /locus_tag="RPM73_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDX6518736.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            8619..9734
                     /gene="macA"
                     /locus_tag="RPM73_000341"
     CDS             8619..9734
                     /gene="macA"
                     /locus_tag="RPM73_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDX6518737.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            9731..11677
                     /gene="macB"
                     /locus_tag="RPM73_000342"
     CDS             9731..11677
                     /gene="macB"
                     /locus_tag="RPM73_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDX6518738.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(11750..11974)
                     /gene="cspD"
                     /locus_tag="RPM73_000343"
     CDS             complement(11750..11974)
                     /gene="cspD"
                     /locus_tag="RPM73_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDX6518739.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            12297..12617
                     /gene="clpS"
                     /locus_tag="RPM73_000344"
     CDS             12297..12617
                     /gene="clpS"
                     /locus_tag="RPM73_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDX6518740.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            12648..14924
                     /gene="clpA"
                     /locus_tag="RPM73_000345"
     CDS             12648..14924
                     /gene="clpA"
                     /locus_tag="RPM73_000345"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDX6518741.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(15268..15355)
                     /locus_tag="RPM73_000346"
     tRNA            complement(15268..15355)
                     /locus_tag="RPM73_000346"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15319..15321),aa:Ser,seq:gga)
     gene            complement(15609..15827)
                     /gene="infA"
                     /locus_tag="RPM73_000347"
     CDS             complement(15609..15827)
                     /gene="infA"
                     /locus_tag="RPM73_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDX6518742.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(16112..16816)
                     /gene="aat"
                     /locus_tag="RPM73_000348"
     CDS             complement(16112..16816)
                     /gene="aat"
                     /locus_tag="RPM73_000348"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDX6518743.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(16858..18579)
                     /gene="cydC"
                     /locus_tag="RPM73_000349"
     CDS             complement(16858..18579)
                     /gene="cydC"
                     /locus_tag="RPM73_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDX6518744.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(18580..20346)
                     /gene="cydD"
                     /locus_tag="RPM73_000350"
     CDS             complement(18580..20346)
                     /gene="cydD"
                     /locus_tag="RPM73_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDX6518745.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(20469..21434)
                     /gene="trxB"
                     /locus_tag="RPM73_000351"
     CDS             complement(20469..21434)
                     /gene="trxB"
                     /locus_tag="RPM73_000351"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDX6518746.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            21978..22472
                     /gene="lrp"
                     /locus_tag="RPM73_000352"
     CDS             21978..22472
                     /gene="lrp"
                     /locus_tag="RPM73_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDX6518747.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22607..26674
                     /gene="ftsK"
                     /locus_tag="RPM73_000353"
     CDS             22607..26674
                     /gene="ftsK"
                     /locus_tag="RPM73_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDX6518748.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            26829..27440
                     /gene="lolA"
                     /locus_tag="RPM73_000354"
     CDS             26829..27440
                     /gene="lolA"
                     /locus_tag="RPM73_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDX6518749.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            27451..28794
                     /gene="rarA"
                     /locus_tag="RPM73_000355"
                     /gene_synonym="mgsA"
     CDS             27451..28794
                     /gene="rarA"
                     /locus_tag="RPM73_000355"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDX6518750.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            28885..30177
                     /gene="serS"
                     /locus_tag="RPM73_000356"
     CDS             28885..30177
                     /gene="serS"
                     /locus_tag="RPM73_000356"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDX6518751.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            30416..32860
                     /gene="dmsA"
                     /locus_tag="RPM73_000357"
     CDS             30416..32860
                     /gene="dmsA"
                     /locus_tag="RPM73_000357"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDX6518752.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            32871..33488
                     /gene="dmsB"
                     /locus_tag="RPM73_000358"
     CDS             32871..33488
                     /gene="dmsB"
                     /locus_tag="RPM73_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDX6518753.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            33490..34353
                     /gene="dmsC"
                     /locus_tag="RPM73_000359"
     CDS             33490..34353
                     /gene="dmsC"
                     /locus_tag="RPM73_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDX6518754.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(34389..35015)
                     /locus_tag="RPM73_000360"
     CDS             complement(34389..35015)
                     /locus_tag="RPM73_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX6518755.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            35330..36478
                     /locus_tag="RPM73_000361"
     CDS             35330..36478
                     /locus_tag="RPM73_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6518756.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            36688..38118
                     /locus_tag="RPM73_000362"
     CDS             36688..38118
                     /locus_tag="RPM73_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6518757.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(38119..39027)
                     /locus_tag="RPM73_000363"
     CDS             complement(38119..39027)
                     /locus_tag="RPM73_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6518758.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            39127..39717
                     /locus_tag="RPM73_000364"
     CDS             39127..39717
                     /locus_tag="RPM73_000364"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDX6518759.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(39799..40539)
                     /gene="pflA"
                     /locus_tag="RPM73_000365"
     CDS             complement(39799..40539)
                     /gene="pflA"
                     /locus_tag="RPM73_000365"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDX6518760.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(40731..43013)
                     /gene="pflB"
                     /locus_tag="RPM73_000366"
     CDS             complement(40731..43013)
                     /gene="pflB"
                     /locus_tag="RPM73_000366"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDX6518761.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(43068..43925)
                     /gene="focA"
                     /locus_tag="RPM73_000367"
     CDS             complement(43068..43925)
                     /gene="focA"
                     /locus_tag="RPM73_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDX6518762.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        11000 a        10823 c        11624 g        10587 t
ORIGIN      
        1 aattccgaag ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg
       61 ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca
      121 gcctttccat gcttcgcgga aacaaaatat cggaataagg aaaaaaacgg caacgacaaa
      181 aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg
      241 ggtgtgacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg
      301 accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg
      361 aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca
      421 taacactgag cctgaatccc catatgcagc gcaataaata atgcccgctc acagtggaaa
      481 cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa
      541 tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg
      601 attaaatctt tgcgcatggt catcggctca ttataacttt gcaatgcgtt atcgccgctc
      661 agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag
      721 cgataatact gattaattac gccagtacga taatattttg ctgttccgag caccacaccg
      781 acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc
      841 atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg
      901 aggaacacgc gctttaacat gcaggagact cgatctaagg aaaatttcca tcaggctact
      961 ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga
     1021 gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa
     1081 gcactcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact
     1141 gaatcaacgg cgcatcggcg ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat
     1201 aacccttgag attcaataga attaacgctc cctgcaacgg tggcagagtc gggttgaatg
     1261 cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac
     1321 cgcttggcga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag
     1381 aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa
     1441 tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc
     1501 ggcccggcag atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc
     1561 agtgaccaca aggcgtgtag ttaacggtga tggctgcaag cgctttttca ccactcaacc
     1621 aggcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc gcaccaataa
     1681 attccatatt ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat
     1741 taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca
     1801 gcgcgtcttc gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca
     1861 aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa
     1921 gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg
     1981 gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca
     2041 acgcgtgaaa cttacatcaa aaatgcgcga cgcatcgcaa attttagccc attactgcca
     2101 gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg
     2161 ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg
     2221 cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat
     2281 taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc
     2341 tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg
     2401 atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag
     2461 tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc
     2521 gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt
     2581 gcagcagatc gtttagcact tcgctacctt taaagtagct gtcgtaagag atgccggtca
     2641 gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact
     2701 tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc
     2761 accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg
     2821 taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga
     2881 aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc
     2941 aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca
     3001 tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct
     3061 gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata
     3121 aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa
     3181 caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat
     3241 taagaaatca gcgcttattt tttcgctccc cggcattacg caatgcgcaa cgaactcagg
     3301 catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgt agacctttaa
     3361 tccgctgacc ataggcgcta acatagcgcc tcattatttt gcgggtgatg atatgcgtgt
     3421 gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt
     3481 taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt
     3541 aaaagttttt catcgcattt gccctgagct acaaaacgcc agccggacac catccggtac
     3601 gctggtgcgc gtgcagttgt tgggtcagtt cccacaatgg ctggcagaga acgccgcccg
     3661 tgcggtggaa ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt
     3721 caacggtagc ggtggtgggg cgacgttact caaagatcct gaactcatct atcagggtgc
     3781 aaaagcgatg cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg
     3841 ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggtgctac
     3901 ggagctggtg gtgcatggac ggacaaaaga gcagggttac cgcgcggagc atattgactg
     3961 gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca acggcgaaat
     4021 ctgggactgg cagagcgcgc aacaatgcat tgcgatcagc ggctgcgacg cagtgatgat
     4081 tggtcgcggg gcgctcaata ttcccaacct gagccgggta gtaaaatata acgaaccgcg
     4141 aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg
     4201 cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga
     4261 atacgatgaa gcaacgggat tatttcagca tgttcgggtg ttgaataatt cccctgatat
     4321 tgcaagggct attcaggcaa ttgatatcga taagctgcga taatagtgga atcgcagttt
     4381 atcagagaca atgaatcgct tattcagctt catcatcttg ttgcgctcct ttcatgagct
     4441 aagccacata ttgccactgg cgcaaggagc gcgcaggggg aggacaatcg cctcctcccc
     4501 ctgcaccccc gggctctggc gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc
     4561 ttcggctacc gggtcgggcg cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc
     4621 atgtgggctg ccccggcctt cggggaacgc ttcagcgatt ttgacgccac caacacccgt
     4681 gcggtttgta acgtcgaaga taaaacaacc agggcagata aaatgccaat gctaattaat
     4741 tacgtttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
     4801 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
     4861 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
     4921 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgtgc
     4981 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gcggaaagca
     5041 tggaataacc gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg
     5101 cgtcaaaacc cgttttacca ctggcaatta aatacagcag cgccgctgca acaataccgc
     5161 cgacaacctg ggcaattacg tagccaacga cttcttttgc cggaaaacgt ccgccagccc
     5221 ataaaccaat agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcaaagg
     5281 ccatcgtcag aacggtcaga ccgaacgcca acgccacgcc ggcaaaacca atgcctaatt
     5341 ccgggaagcc tgcggccagt acagcactac cacagccacc aaaaacaagc cagaaagtac
     5401 caaaacattc agctgttaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg
     5461 aaaaatatgg tcgttttata gggtcgtcgt aaaaaagtga cgacggaaat aatgcgcggc
     5521 tattttaaaa acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag
     5581 ggcaacagcg ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa
     5641 gatttaatcc tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg
     5701 tttttgcaga cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca
     5761 tttgccatgc ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc
     5821 agcgtaaagg ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt
     5881 gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt
     5941 ggggagaacg cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca
     6001 gaatcagatc tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac
     6061 gggcgagaac atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat
     6121 cgggttcgcc gttatcggcc gctggaagag tgctggacgc catgcaccga tggctatcac
     6181 cgtatttttt atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc
     6241 agttttctcg ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc
     6301 catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt
     6361 cgtaacggca cggtgccaaa tgtccctaat gtggaagtca ccgcgcgcca gctcgatttc
     6421 ctcgcccgtg agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga
     6481 ctttccgcaa tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg
     6541 cgatatcgtt taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat
     6601 atcatcaacc ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc
     6661 ctgtttgcta ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca
     6721 ttgttgctga tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg
     6781 catctgttga atcttctgcc attgcagcgc attgccacca ccaactcggg tgagttgctt
     6841 tcgttaacgc cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc
     6901 tggcgtctgg ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt
     6961 cgttttaacc gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg
     7021 gaaacctggg ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg
     7081 atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga
     7141 atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct
     7201 acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta
     7261 ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc
     7321 gtggcgcaaa tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc
     7381 catcgctctt ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt
     7441 gtggactccg taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc
     7501 ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc
     7561 tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt
     7621 gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc
     7681 cagaaagcgt tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg
     7741 taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac
     7801 agacaggccg cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca
     7861 ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc
     7921 gttcttttcc cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg
     7981 ctgttgcgaa acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc
     8041 tcaagcgtga actgctcgcc gtttttgcct tccagtttag ccagttgcag cccgggggta
     8101 ttcaaatata agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa
     8161 cgtaacgcct ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga
     8221 cgcacaggca gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt
     8281 tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg
     8341 cgaaaactac ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga
     8401 aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac
     8461 atattcatga aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa
     8521 acatcaatag tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt
     8581 aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga
     8641 agaagcgtta cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa
     8701 ttcttaacgc acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc
     8761 aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg
     8821 tcagcggtca gttgaaaact ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc
     8881 ttttaggggt tattgatcct gaacaggctg aaaaccagat taaggaggtc gaagcaacgc
     8941 tgatggagct acgtgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga
     9001 cgtattcccg tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca
     9061 ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca
     9121 agcgcaatca ggcctctctc gatacggcta aaaccaatct cgattacact cgcatcgttg
     9181 ccccgatggc cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg
     9241 cacaacaagc accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc
     9301 aggtttctga agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac
     9361 ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa
     9421 aggttaacga cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc
     9481 tgcggctgga tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga
     9541 cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt
     9601 tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg
     9661 ttgagattgt caaagggctt gaagcgggcg atgaagtggt gattggtgag gccaaaccag
     9721 gagctgcaca atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg
     9781 tgatgagcag gttgaggtgc tgaagggcat caccctcgat atttatgccg gtgagatggt
     9841 agcgattgtt ggcgcttcag gttccggtaa atcgaccctg atgaatattc tcggctgtct
     9901 ggataaggcc accagcggca cctatcgcgt cgccggtcag gatgttgcca cactggacgc
     9961 cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt
    10021 actttcgcat ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga
    10081 gcggaaacag cggctgcttc gcgcccaggg gttactgcaa cgactggggc tggaagaccg
    10141 tacagagtat tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg
    10201 ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag
    10261 ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt
    10321 gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg
    10381 cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac
    10441 ggagcctgtt gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc
    10501 gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct
    10561 ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa
    10621 acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg
    10681 gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat
    10741 cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg
    10801 cctgcgttat aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt
    10861 taatgtctat ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg
    10921 tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc
    10981 agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc
    11041 ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag
    11101 cacgatgtcc gggcgagtta tgggccagtc gtggctgaac tccattactg tcagggtaaa
    11161 agaaggattt gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca
    11221 cggaaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac
    11281 cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg
    11341 tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat
    11401 tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt
    11461 actggtttgc ctggtgggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac
    11521 cttgcagctt ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc
    11581 gtttctctgc tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc
    11641 acgactggat ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc
    11701 gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg
    11761 ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga
    11821 catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg
    11881 aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg
    11941 cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat
    12001 acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct
    12061 agaaatacaa tttagagaat tagggcgagc cgtcaagcgt ttgacagggg ccagggggca
    12121 ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct
    12181 gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc
    12241 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
    12301 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
    12361 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt
    12421 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
    12481 tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag
    12541 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
    12601 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
    12661 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
    12721 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
    12781 aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
    12841 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
    12901 agcgtgtact gcaacgtgcg gtcttccatg tccaatcctc cggtcgcaat gaggtaaccg
    12961 gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc
    13021 gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
    13081 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
    13141 gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa
    13201 tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc
    13261 gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg
    13321 aaggtcttgc ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga
    13381 tttactctct cgatatcggt tctctgttag cgggcactaa atatcgcggc gactttgaaa
    13441 aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg
    13501 atgagatcca caccattatc ggtgcggggg cagcgtctgg tggccaggtc gatgcggcta
    13561 acctgatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
    13621 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
    13681 atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
    13741 atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcagcg gtagagctgg
    13801 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgacgtt atcgacgaag
    13861 cgggcgctcg cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
    13921 atattgagtc cgtggtggcc cgtattgcgc gcattccaga gaagagtgtt tctcagagtg
    13981 accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata
    14041 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
    14101 ataaaccggt tggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa acagaggtga
    14161 cggtacagct ttcgaaagcg ttgggcattg agctgctgcg ctttgatatg tccgagtata
    14221 tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc
    14281 agggaggttt gctgactgat gcggtcatca agcatccaca tgcggtgctg ttgctggacg
    14341 aaatcgagaa agcgcaccct gacgtgttca atattctgtt gcaggtgatg gacaacggta
    14401 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca
    14461 ccaacgccgg ggtacgtgaa actgagcgta aatccattgg tcttatccac caggataaca
    14521 gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg
    14581 acaacattat ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat
    14641 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
    14701 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
    14761 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc
    14821 tggtggacgg cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg
    14881 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgttaggtag
    14941 gttggtcaag tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaggag
    15001 gttcagaccc tttttttaat gatgatggta agttgttgat aattagtgct gcgggaaggt
    15061 aaggataaaa aagggtgctg caggagaatg ggatggtttt gctttattaa caacgggcta
    15121 aacgtgtagt atttgagttc actgccgtac aggcagctta gaaatcgacg gggtgcggta
    15181 aaacctttgc gaacgcgttc actgccgtac agacagataa aatgcgaaaa aaaagctcgc
    15241 actttcgtac gagctcttct ttaaatatgg cggtgagggg gggattcgaa cccccgatac
    15301 gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt
    15361 gttttgctac caaacctcat gggtggcaac ggggcgctac tatagggagt tggagtaaaa
    15421 cggtcaagaa gaattttaat gataattatt gtttgctcat actgtaaaca agttgtgcag
    15481 tatatctaca tcgagacaag ttacggactt atacttccaa agtacttcat acatatcaca
    15541 aaataaaaag gccggttaaa ccgacctttt actcgttctt tctcttcgcc catcaggcgg
    15601 taaaacaatc agcgactacg gaagacaatg cggcctttgc tcaggtcgta cggggtcagt
    15661 tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat tttaccggag
    15721 atgtgtgcag taaccacgtg accgttttct aactctacgc ggaacatggt attaggcaac
    15781 gtttcaagaa cggtaccttg catttcaata ttgtcttctt tggccatcta atcctctggg
    15841 gtatcactac cgtaatttga accggcaaga taatgccgaa gttctgtaaa taagtaaaga
    15901 tttgcgcgct aaatcgcaac aaacaggttc ggcacattac tccgaaaaca cacggctaag
    15961 ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa acgatgggcg ttacctgacg
    16021 cgaaaaaatc cttatcggca gcggggtaat gagcgtaacc aactctgcga ccgcaattat
    16081 aacactctgg ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac aagcatcgtg
    16141 gtacccagaa attattcggc aatcgtccga ggcgcatttg attgagataa ttaaggtaat
    16201 cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg acctggcagt
    16261 cgataagctt accgccatga ccgataaatt cctcacagaa taccagaagc gccgttttag
    16321 acgcattttc catccggctg aacatggact cgccacaaaa tagcgttccc tgggccacgc
    16381 cgtacatacc gccgacaagc tcatcttcac gccagacttc aatggagtgg gcatggccga
    16441 gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc
    16501 gatcgctggc acagccttca atgacctgac caaaagcgta attcatcgtg acacgatagg
    16561 gcgagcgttt atgaaatcgc ttcatactac ggctgatatg cagtgattct ggccatagca
    16621 ccgcgcgggg atcgggcgac caccagagga tggggtcgcc tggagaaaac cacggaaaaa
    16681 taccacgctg gtaagccatt aacaggcgcg cagggctaag atcgccccca agtgccagca
    16741 ggccgttagg ctcacgtaat gcgccttccg gggaagggaa ggctattgaa tggcgagaaa
    16801 gctgaaccag gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa taatagctta
    16861 caaaccctgc ttgaactggt aataacgccc ctgtctggca agcagctctg cgtgagtacc
    16921 ttgctcaata atttgcccgt tgtccatcac tattatttgt tggaaacgag agagtccgcg
    16981 aagtcgatgg gtgaccatta acaccgtttt ctcacgcatc atttctgcaa gcaattcaag
    17041 gatctggctt tcggttgtgg catctaagcc ttcggtaggt tcatccagca acaccagtgg
    17101 cgcatcatgt aacagcgcac gggcgatagc cagacggcgc agttcaccac cggagagctg
    17161 gcgtccgcct tcacctaacc aactgttgag acctgcatcc tcgagcagct tctccagacc
    17221 aacgcgacgc aagctctccg ccagagcctc atcactactg ccaggcgagg cgagtaacag
    17281 attatcacgc agcgtggcgc taaacagatg cactcgctga ggaacaacgc tgatggtctg
    17341 tcgtagagcc gcttcattca ggctggctat ggggctatcg ttaagcaaaa tctctccctg
    17401 ttgcgggtcc caagcgcggg tcagcagttg taacagtgtt gatttgccgc atccggttcg
    17461 cccgagaatc gcaatatgtt ccccggcgtt tacctgaaga gaaatccctt taagtgcctg
    17521 ttgggattgc tccggataag tgaactgaac atcccgtaat gtcagcgaaa cgcgatcagc
    17581 aacacgagtt tgggtatcag ggaaggtgac ttccggtttt tgatccgtta aatcagtgat
    17641 ccgaacggca gaggcaatga cttgccccag atgctgaaat gcacccgtta ctggtgccag
    17701 tgcttcaaac gcggctaacg cgcagaagac aaacagggca attaacgcgc cgggttgagc
    17761 attgccgcca acgccgccag acgccatcca cagcatcagg atcaccgcta acgcgccaat
    17821 gagcagcatt atcgcttgcg acaatgcggt cagttcagat tgacggcgtt gcgcttccag
    17881 ccattgaatt tctgtattct ctagttgcgt gcgataacga tcgctggcgc caaaaatggt
    17941 cagctcagct tgcccttgca gccaggccgt cagttgttgg cgatactgtc cgcgaagatg
    18001 agtcagattt tgcccggtgc tttttcccgc acgataaaac agcggtggca tcaggaaaag
    18061 cgtcagtaac ataatgccgc ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc
    18121 gattgtcacc accataatca ccacaaaagc gcccaccagc ggcgagataa cgcgcagata
    18181 aagatgatcg agcgtatcaa catccgccac cacgcgattg agcaattcgc cctgacgata
    18241 gcgcgccagt ccggcagggg agaggggcag caatttgctg aaggtgtaaa tgcgcagatg
    18301 ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt tcaaaataac gcccggcagt
    18361 acgggtgatt gctgcgccac gcacgcccgc agcgggtagc atatagttga agctgtacag
    18421 tccggcaacc cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg tcaacagacc
    18481 gatactggcg agcagcgtga caattgccag cacgatacca agacttaaca tccatttatg
    18541 acgtttatac agtgccagat agggtagcaa agcgcgcatt taaatctcct cctgacgatg
    18601 ggccagtaat gtggcgaatg ggccaccagc cacacttaat tccgcgtaac gtccttgctc
    18661 aataatccgg ccatcctgca taacccaaat gacatcccag tcagcaagat cttctaactg
    18721 gtgggtgacc attaacgttg tctggcgcag agaggcggca ttcagcgcct ccattacgcg
    18781 ctgttcactg tgagcatcaa ggctggcagc gggttcatcc aacagtaata gcgaacaggg
    18841 atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc
    18901 ctggtcgcca acaggtgtat caacgccttg tggcaggagc ggtagaaact cgctgaccca
    18961 ggcgttatcc agcgctgctt gtaattcttg ttcgctggca tcaggtcgcg ccagtagtac
    19021 gttatcccgc aatgttgctg ccggtaattg tgggttttgc ccaacccagg agagatgttt
    19081 acgccatgat tctggtgata aatcgcgtaa ttctatcccg ttgattcgta atgatccctg
    19141 atatgagaga aaaccagaaa gcgcgttcag cagtgagctt ttacctgaac cgctgcgacc
    19201 aaccaacact gcacgttggc ctgctggcaa agtaaagttc agcggtccgg ccagcgtttt
    19261 accttccggc gacgtgataa acagatcctc ggcctcaatg gtcaccggat cggtcgatgc
    19321 taattccacc tcgccacgct gcggatgggc gagcggggtt tccataaacg ttttcagact
    19381 gtcagctgca ccaacagcct gggctttagc atgataaaac gtaccgagat cgcgcaatgg
    19441 ctggaaaaac tctggcgcaa ggatcagggc cagaaaaccc gcagccagcg tcacaccggt
    19501 atcgtagtga ccaaaatcca gctctccgag ataggaaaaa ccaaagtaga ccgccaccag
    19561 agcaattgac agcgaggtaa aaaattcgag aatgccggag gataaaaacg ccagccgtag
    19621 cacttccatt gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt caatttcagc
    19681 ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc
    19741 acttaagcga gcaagagcga gaaagttacg ccggttagca tcggcagccc ccatcccaac
    19801 cagcgccata aacaacggaa ttaacggtgc tgtgcccagc agaatgagcg ccgcagccca
    19861 gttagagggg aagatcgcca ccacaatcag caacggcacc gacactgcca gcgccatttg
    19921 cggcaggtag cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta ccaacgtcgc
    19981 ccagctcccc gcaggtttac cctgaatcca cgctggccct gcttgttgca gacggtcgag
    20041 aacctgacgg cggatggcaa agcggatatg ctgcccggcg tgataaccca cccgttcgcg
    20101 taaccagacc acccatgcgc gcagtacaaa ggtcagaacc agtaacgtaa agggaagcag
    20161 cagggcttca cggggaatat tctccataat catatgttgc agaattcgcg ccatgaacca
    20221 ggcctgggca atgatcaata tgccgcttac aaagcccagc agacgagaaa tattcagcca
    20281 acgttgggag atgacgcttt gctgttttaa ccagcgggtt aactcttttt gacgggattt
    20341 attcattgca cgcttagcag gtgagttatc agaattattt gcagagcaat gttacaacgg
    20401 ggaaaaaata aaggcgaccc atagtcgcat ggtgtcgcct tctttacttt tgttactgat
    20461 ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc gttccgcatc aagtgctgcc
    20521 atgcagcctg taccggccga agtaatggcc tgacgataaa tatgatccat cacgtcaccc
    20581 gcggcaaaga cgccaggaat gctggtctgg gtggcattac catgaatacc cgactgcact
    20641 ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa tggcagtatt cgggctgtga
    20701 ccgatagcaa caaacagacc agcaacgtcg agtgactcga tgttatcgct gttttgcgta
    20761 tcgcgcagac gaacgccagt gacacccatt tgatcgccgg tcacttcttc cagcgtacgg
    20821 ttggtgtgca gaatgatgtt gccgttctcc actttatcca tcagacgctt aataaggatt
    20881 ttttccgcgc ggaaaccgtc acggcggtga ataagatgca cttccgaagc gatgttagac
    20941 agatacagcg cctcttcaac cgcggtattg ccgccgccga tgaccgcaac tttctggttg
    21001 cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt aaaggcttct
    21061 tcagagggca ggccgagata gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa
    21121 gtgtattcgc cgttatcgcc attcagacgg aacggacggt tttgcagatc caccttgttg
    21181 atatgatcaa aaatgatctc agtttcaaac ttggtggcat gttcgtgcat gcgctccatt
    21241 aataacggac cggtcagatc gtttggatcg ccaggccagt tttccacttc cgtggtggtg
    21301 gtcagttggc cgcctttttc catgccggta atcagcacag gttgcaggtt ggcgcgcgcc
    21361 gcgtagacag cagcggtgta tcccgccggg cctgaaccca ggataagcag tttactgtgt
    21421 ttggtcgtgc ccatgagatc cccatagttg ttggcagaca atgggcagga ttgtagggaa
    21481 tttacagacg taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa tcggcagcat
    21541 cgataagcag gtcaaattct cccgtcatta tcacctctgc tacttaaatt tcccgcttta
    21601 taagccgatt aaatgatgaa taaacgcccc tgttaatgaa tatctggcat gttgtactaa
    21661 aaatcgatgt tttgctttga caatcccctg gtgttttgcg aaaacattcg aggaagaaaa
    21721 aaacagtatt cttatatgcg cataaccatg catgtaaata ccatgtttac cgtgctagtg
    21781 aaatctacgt atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac
    21841 ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc
    21901 gtgacgggta gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat
    21961 acagagagac aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta
    22021 tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt
    22081 ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac
    22141 aagggtttat tcagggctat acggcgctgc tgaaccccca ttatctggat gcatcacttc
    22201 tggtattcgt tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata
    22261 ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact
    22321 acctgttgaa aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc
    22381 tgctgcgtct gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc
    22441 agagtaatcg tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt
    22501 ttgattacac tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc
    22561 cgttttagca tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata
    22621 cactgaagac aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc
    22681 gttgctgatc cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa
    22741 cccttcggac cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg
    22801 gatgcccggt gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac
    22861 cattcccgtc attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga
    22921 atacattgat tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac
    22981 ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt
    23041 cattggcagc ttactaagta ctacgctaca accactgcta cacagtagcg ggggaactat
    23101 tgcgctgctc tgcgtttggg ctgcgggcct gacgctgttc accggttggt catgggtgac
    23161 cattgctgaa aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac
    23221 ccgtcgcgat gatacctggg tcgatgaaga tgaatatgaa gatgacgaag agtatgaaga
    23281 tgaaaaccac ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc
    23341 gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc
    23401 gttgttctcc ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt
    23461 ggctgccgac ccggacgacg tcctattttc gggcaatcgt gcaacgcagc cagaatatga
    23521 cgaatacgat ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc
    23581 tgctaccacg gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc
    23641 tgttgcctct gttgatgttc caccctcgca acctacagta gcctggcagc ctgtaccggg
    23701 tccacaaacg ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca
    23761 atatgcgcag cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca
    23821 gccgtattat gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc
    23881 ggaacaacct gttcagcaac cgtattacgc ccctgcgcca gaacaaccgg tggcaggtaa
    23941 cgcctggcaa gccgaagagc agcaatccac ttttgctcca cagtctacat accagactga
    24001 gcaaacttat cagcaaccag ccgctcagga gccgttgtac caacagccgc aatccgttga
    24061 acagcagcct gttgtggagc ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc
    24121 gctttactat tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc
    24181 ctggtatcaa ccgattccag aaccggttaa agaaccagaa ccgatcaaat cttcgttgaa
    24241 agcgccttct gttgcagcag tacctccagt agaagccgct gccgctgttt ctccgctggc
    24301 atctggcgtg aaaaaagcga cactggcgac gggggctgcc gcaaccgttg ccgcgccagt
    24361 cttcagtctg gcaaatagcg gtggaccgcg tccgcaagtc aaagagggga ttggcccgca
    24421 gttgccacga ccgaaacgta tccgcgtgcc aactcgtcgt gaactggcgt cttacggtat
    24481 taagctgccc tcacagcgtg cggcggaaga gaaagcccgt gaagcccagc gcaatcagta
    24541 tgattctggc gatcagtata acgatgatga aatcgatgcg atgcagcagg atgaactggc
    24601 acgtcagttc gcccagacac agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc
    24661 cgtaaacgca gaagatgcag atgctgcggc agaggctgaa ctggctcgtc agtttgcgca
    24721 aactcaacaa caacgttatt ccggcgaaca accggctggg gcgaatccgt tctcgctgga
    24781 tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt
    24841 tacgccaatt gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc agcagcagta
    24901 tcagcagccg caacaaccag ttccgccaca gcagcaatat cagcagccgc aacagccggt
    24961 tgcgccgcag ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca
    25021 gcagccgcaa caaccggttg cgccacagcc gcaatatcag cagccgcaac aaccagttgc
    25081 gccgcagccg cagtatcaac agccgcaaca accggttgca ccgcagcagc aggataccct
    25141 gcttcatccg ctgttgatgc gtaatggcga cagccgtccg ttgcataaac cgacgacgcc
    25201 gctgccttct ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg tagatacctt
    25261 tgcgcttgaa caaatggctc gcctggtgga agcgcgtctg gctgatttcc gtattaaagc
    25321 cgatgtcgtc aattactctc cggggccggt tatcactcgc tttgaactta acctggctcc
    25381 tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac cttgcccgtt cactttcgac
    25441 ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc tggagttacc
    25501 aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg gataacgcca aattccgcga
    25561 taatccgtcg ccattaaccg tggtgctggg taaagatatc gccggtgagc cggtggttgc
    25621 cgatctggcg aaaatgccgc acttgttggt tgcggggact accggttccg gtaaatctgt
    25681 cggtgtgaac gcgatgatcc tgagcatgct ttataaagca cagccagaag atgtgcgttt
    25741 catcatgatc gacccgaaaa tgctggagct ttcggtttat gaaggcattc cgcatctgtt
    25801 aacggaagtc gttactgata tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga
    25861 gatggagcgt cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa
    25921 cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt ccagacccgt actggaagcc
    25981 gggtgacagt atggatgccc agcatccggt gctgaaaaaa gaaccataca ttgtggtgtt
    26041 ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag agctgatagc
    26101 acgtctggcg caaaaagccc gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc
    26161 atcggttgat gttattactg gtctgattaa agcgaatatt ccgacccgta tcgcctttac
    26221 cgtatccagt aagattgact cacgtaccat tcttgatcag gctggcgcgg aatcactgct
    26281 ggggatgggg gatatgctct actctgggcc gaactccacg ttgccggtac gtgtccatgg
    26341 tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg
    26401 cccacagtat gttgatggca tcacttccga cagtgaaagc gaaggtggcg cgggcggttt
    26461 cgatggcgct gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa
    26521 acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc attggttata accgtgcagc
    26581 gcgtattatc gaacagatgg aagcgcaggg gattgtcagc gaacaggggc acaacggtaa
    26641 tcgtgaagtg ctggccccac cgccgtttga ctaattaatg cattgccgga taaggcgcgg
    26701 tagcgtcgca tccggcactc tatcaactga aaattcagta ttttcttctt tcctcaagct
    26761 gattattagc ctggaataga gagtagaggg aactcccgat cgggagtgac gtaatttgag
    26821 gaataatgat gaaaaaaatt gccatcacct gtgcattact ctcaagctta gtagcaagca
    26881 gcgtttgggc tgatgccgca agcgatctga aaagccgcct ggataaagtc agcagcttcc
    26941 acgccagctt cacacaaaaa gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg
    27001 gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca tatgacacaa cctgatgaaa
    27061 gcattctggt ttctgacggt aaaacactgt ggttctataa cccgttcgtt gagcaagcta
    27121 cggcaacctg gctgaaagat gccaccggta atacgccgtt tatgctgatt gcccgcaacc
    27181 agtccagcga ctggcagcag tacaatatca aacagaatgg cgatgacttt gtcctgacgc
    27241 cgaaagccag caatggcaat ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa
    27301 tccatcagtt tagcgcggtg gagcaggacg atcagcgcag cagttatcaa ctgaaatccc
    27361 agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg
    27421 tagatgatca acgtaagtag aggcacctga gtgagcaatc tgtcgctcga tttttcggat
    27481 aatacttttc aacctctggc cgcgcgtatg cggccagaaa atttagcaca gtatatcggc
    27541 cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc cgggcattta
    27601 cattctatga tcctctgggg gccaccgggt accggcaaaa caaccctcgc tgaagtgatt
    27661 gcccgctatg cgaacgctga tgtggaacgt atttctgccg tcacctctgg cgtgaaagag
    27721 attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg cactattctt
    27781 tttgttgacg aagtgcaccg attcaacaaa agccagcagg atgcatttct gccacatatt
    27841 gaagacggca ccatcacttt tattggcgca accactgaaa acccgtcgtt tgagcttaat
    27901 tcggcactgc tttcccgtgc ccgtgtctat ctgttgaaat ccctgggtac agaggatatt
    27961 gagcaagtac taactcaggc gatggaagac aagacccgtg gttatggtgg tcaggatatt
    28021 gttctgccag atgaaacacg acgcgccatt gctgaactgg tgaatggcga cgcgcgccgg
    28081 gcgttaaata cgctggaaat gatggcggat atggccgaag tcaatgatag cggtaagcgg
    28141 gtcctgaagc ctgaattatt gaccgaaatc gccggtgagc gaagcgcccg ctttgataac
    28201 aaaggcgatc gcttttacga tctcatttcc gcactgcata agtcggtacg tggtagcgca
    28261 cccgatgcgg cgctctactg gtatgcgcga attattaccg ctggtggcga tccgttatat
    28321 gtcgcgcgtc gttgcctggc aattgcttcc gaagatgtcg gcaatgccga cccacgggcg
    28381 atgcaggtgg caattgcggc ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa
    28441 cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac
    28501 actgcgttta aagctgcgct ggcggatgct cgcgaacgtc cggattatga cgtgccggtt
    28561 catttgcgta atgcgccgac gaaattaatg aaggaaatgg gctacgggca ggaatatcgt
    28621 tacgctcatg atgaagcaaa cgcttatgct gccggtgagg tttacttccc gccggaaata
    28681 gcacaaacac gctattattt cccgacaaac aggggccttg aaggcaagat tggcgaaaag
    28741 ctcgcctggc tggctgaaca ggatcaaaat agccccataa aacgctaccg ttaatgttat
    28801 cgttgcggta atgttgttac tgtatccctg tggtcgcagg ctgtggccac atctcccatt
    28861 taattcgata agcacaggat aagcatgctc gatcccaatc tgctgcgtaa tgagccagac
    28921 gcagtcgctg aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct
    28981 cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac
    29041 tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt
    29101 ctggaagtga acaaactggg cgaagagctg gatgcagcaa aagccgagct ggatgcttta
    29161 caggctgaaa ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg
    29221 gtaggtaaag acgaaaatga caacgtcgaa gtcagccgct ggggcacccc gcgtgagttt
    29281 gactttgaag ttcgtgacca tgtgacgctg ggtgaaatgc actctggcct cgactttgca
    29341 gctgcagtta agctgactgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg
    29401 caccgcgcac tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag
    29461 aactatgttc cgtacctggt taaccaggac acgctgtacg gtacgggtca gctgccgaaa
    29521 tttgctggcg atctgtttca tactcgtccg ctggaagaag aagcagacac cagtaactat
    29581 gcgctgatcc caacggcaga agttccgctg actaacctgg tgcgcggtga aatcatcgat
    29641 gaagatgatc tgccaattaa gatgaccgcc cacaccccat gcttccgttc tgaagccggt
    29701 tcatatggtc gtgacacccg tggtctgatc cgtatgcacc agttcgacaa agttgaaatg
    29761 gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg aagagatgac tggtcatgca
    29821 gaaaaagtcc tgcagttgtt gggcctgccg taccgtaaaa tcatcctttg cactggcgac
    29881 atgggctttg gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc
    29941 taccgtgaga tctcttcctg ctccaacgtt tgggatttcc aggcgcgtcg tatgcaggca
    30001 cgctgccgca gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt
    30061 ctggctgttg gtcgtacgct ggttgcggtt atggaaaact atcagcaggc tgatggtcgt
    30121 attgaagtac cagaagttct gcgtccgtat atgaacggac tggaatatat tggctaatac
    30181 ccaatttttc tgaatctaaa aagcgcctgc gggcgctttt tttgtctccc tttgataccg
    30241 aacaataatt actcctcact tacacgtaat actactttcg agtgaaaatc tacctatctc
    30301 tttgattttc aaattattcg atgtatacaa gcctatatag cgaactgcta tagaaataat
    30361 tacacaatac ggtttgttac tggaatcaat cgtgagcaag cttgagtgag ccattatgaa
    30421 aacgaaaatc cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt tggtaaaaac
    30481 gacagcgatc ggcggcctgg caatggccag cagcgcatta acattacctt ttagtcggat
    30541 tgcgcacgct gtcgatagcg ccattccaac aaaatcagac gaaaaggtta tctggagcgc
    30601 ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgtcgtgg acggtgaaat
    30661 caaatatgtc gaaacggaca ataccggcga tgacaattac gacggcctgc accaggttcg
    30721 cgcctgtctg cgtggacgtt ccatgcgtcg ccgtgtctat aatccggacc gcctgaaata
    30781 tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta gctgggaaga
    30841 agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg gcaacgagtc
    30901 tatctatctg aactatggca ccggtacgct gggcggcacc atgacccgct cctggccgcc
    30961 gggaaatacc ctggtcgcgc gcctgatgaa ctgctgcggc ggttatctta accattacgg
    31021 tgactactct tctgcgcaaa ttgcggaagg tttgaactat acctacggcg gctgggcaga
    31081 tggtaacagc ccgtcggata tcgaaaacag taaactggta gtgctgtttg gtaataaccc
    31141 tggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg cacgccagaa
    31201 atctaatgcc cgcatgatca tcatcgatcc gcgctatacc gacaccggtg ccgggcgcga
    31261 agatgagtgg atccctattc gtccgggaac agatgccgca ctggttaacg gtctggcgta
    31321 cgtcatgatc actgaaaacc tggtggatca ggcattcctc gataaatatt gcgttggcta
    31381 cgatgagaaa accctgccag ccagtgcgcc gaaaaatggc cactataaag cttatattct
    31441 gggtgaaggg ccagatggcg tggctaaaac accggaatgg gcctcacaaa tcaccggtgt
    31501 tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac cggcgtttat
    31561 cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc
    31621 gatgctggcg attctgaccg gtaacgttgg tattaacgga ggcaacagcg gcgcgcgtga
    31681 aggttcatac agcttaccgt ttgtccgtat gccgaccttg gaaaacccga tccagaccag
    31741 catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg
    31801 tgatggtgta cgcgggaaag ataagctgga tgtgccgatc aaaatgatct ggaactatgc
    31861 cggtaactgc ctgattaacc agcattctga aatcaaccgt acccatgaaa tccttcagga
    31921 tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat cggcgaaata
    31981 tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg cgctggatgc
    32041 atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac cgcgctttga
    32101 atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg ttgagcaaca
    32161 gtttactgaa ggccgtacac aggaagagtg gatgcggcat ctgtatgccc agtcgcggga
    32221 agcgattcct gaactgccaa cgtttgaaga gttccgcaag caggggatct ttaaaaagcg
    32281 cgacccacaa gggcatcacg ttgcttataa agccttccgt gaagatccgc aggcaaaccc
    32341 actgactacg ccatcgggca aaattgagat ttattcgcag gcgctggctg acattgccgc
    32401 tacctgggaa ttgccagaag gcgatgtgat cgatccactg ccgatctaca cgccgggctt
    32461 tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacgg gtttccacta
    32521 taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg cttgccgtca
    32581 ggaaatgtgg atcaacccgc ttgatgcgca aaaacgcggt atcaacaacg gcgataaagt
    32641 caggatcttt aacgatcgtg gtgaagttca tattgaggcg aaagtgacgc cacgaatgat
    32701 gccgggtgtg gtcgcactgg gtgaaggtgc ctggtatgac ccggatgcaa aacgtgtcga
    32761 taagggcggt tgtattaacg tactgaccac tcaacgtccg tctcctctcg ctaaggggaa
    32821 tccgtcacat acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc
    32881 agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc gagctggcct
    32941 gtaaagacta caaagatttg acgccagaag tcagcttccg ccgcatttat gaatatgctg
    33001 gcggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt
    33061 cgatttcatg taaccactgc gaagatccgg cttgtactaa agtctgcccg agcggtgcga
    33121 tgcataaacg cgaagatggt tttgttgtgg tcgatgaaga tgtatgcatt ggctgccgct
    33181 actgtcatat ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa ggccatatga
    33241 ccaaatgcga tggttgttat gaccgtgttg cggagggtaa aaagccgatc tgtgttgaat
    33301 cttgcccgct gcgggcgctg gatttcggtc ctatcgacga gctgcgtaaa aaacatggcg
    33361 atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat attgtgatca
    33421 aacccaatgc caacagccgc ccgaccgggg ataccactgg ctacctggca aacccgaagg
    33481 aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac ggtcttcggg
    33541 caatgtgtag cgggtggttt tatcgttctg gctttggcgc tgctcaaagg cgacctgcga
    33601 gcagaagccc agcagcgtgt tatcgcctgc atgtttggct tatgggtgct gatgggcatt
    33661 ggctttatcg cctctatgct ccatcttggt tcaccgatgc gcgcctttaa ctcgctcaac
    33721 cgggtagggg cttcagcact cagtaacgaa atcgccagcg gttcgatctt ttttgctgta
    33781 ggcggcatcg gctggttgct ggcaatgctg aaaaagctgt caccggcatt gcgtacgcta
    33841 tggctggtgg tgacgatggt tcttggcgtc atctttgtct ggatgatggt gcgtgtgtat
    33901 aacagcattg ataccgttcc gacctggtac agcatctgga cgccgatggg cttcttcctg
    33961 acgatgttta tgggcggccc gttactgggt tacctgttgt tgagtctggc tggcgtcgat
    34021 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    34081 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    34141 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    34201 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    34261 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    34321 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    34381 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    34441 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    34501 agctcgcacg ccacgccgaa ccatgtcatc aattgcgcac ctgcttgcga catgcgatcc
    34561 catgccgaat gccgggtaat ttcattaaag gtccctgacg cgtcagtcac aacaaagaca
    34621 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    34681 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    34741 tcccaggcgt taatatttcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    34801 agttctggaa ctaatggacc gttaggaccg gtttcaaaac tggtggtaag aatagtaggt
    34861 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    34921 tcgatatccc gtacaaggga aagtaaacca gcctgatgat caacaagcaa aacggcagca
    34981 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    35041 gtacgtctga aatcggtcag acgtgatgca ttgctctgaa agcatagacg ggaaatatga
    35101 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    35161 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    35221 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    35281 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    35341 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    35401 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    35461 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    35521 cattaaacgc attggcttta accgcagcta ttatctggcc tccttcattt ttgccgctgg
    35581 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    35641 tggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    35701 gtcacgtaac cgtgggcgtt tgcttgctgc atacatgatg gtttattacg tgggaacgtt
    35761 tttaggccag ttactggtca gcaaggtctc aaccgagctg atgagcgtat tgccgtgggt
    35821 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtgc tgaatcagca
    35881 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    35941 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    36001 gttgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    36061 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    36121 actgctggtg ttgcgtgttc aggtctttgt cgtcattctc ggcagtatcg cgatgcttag
    36181 ccaggcggcg atggcccctg cgttattcat cctcggtgcc gctggcttta cgctatatcc
    36241 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    36301 ggccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta ccgctatgct
    36361 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    36421 tttgctgatg ctgctgcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    36481 gaattcaagc agagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    36541 acttgcttta ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    36601 gcatttcgcg atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    36661 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcaggaaaa acagttgcga
    36721 tggtacaaca ttgcgctgat gtcttttatc actgtctggg gttttggcaa cgttgttaat
    36781 aactatgcca accaggggct ggtggttgtt ttttcatggg tgtttatctt tgcactctat
    36841 ttcacacctt atgcgctaat tgttggtcag ttaggctcga ccttcaaaga tgggaagggc
    36901 ggggtcagta cctggattaa acacacgatg ggacccggac tggcttatct cgccgcgtgg
    36961 acctactggg tggtgcatat tccctatctg gcacaaaaac cccaggcaat tctgattgcg
    37021 ctcggttggg cgatgaaagg cgacggttcg ctaatcaaag aatattcagt cgtagcgtta
    37081 caggggttaa cgctggtgct gtttatcttc tttatgtggg ttgcttcacg cggtatgaaa
    37141 tcgctgaaaa tcgtcggttc tgtggcaggg attgcgatgt ttgttatgtc gctcctgtat
    37201 gtggcgatgg cggtaaccgc gcctgcgatt actgaagtgc atattgcgac cacaaacatt
    37261 acctgggaaa cgttcattcc tcatatcgac tttacctaca ttaccactat ttcaatgctg
    37321 gttttcgcgg ttggcggagc agagaagatt tctccttacg ttaatcaaac gcgcaaccca
    37381 ggaaaagaat ttccaaaagg gatgctatgc ctggcggtga tggttgcggt ttgtgccatt
    37441 ctgggctcac tggcgatggg gatgatgttt gactcgcgta atatcccgga tgacttaatg
    37501 acgaacggtc aatattacgc ctttcagaag ctgggcgagt attacaacat gggtaatact
    37561 ttaatggtga tttacgccat tgcgaatacc ctgggacaag tagctgcgct ggtattctcg
    37621 attgatgccc cgcttaaagt gctattaggt gatgctgaca gcaaatatat tccagccagt
    37681 ttatgtcgta ccaacgcttc tggtacgccc gttaatggct attttctgac cctggtgctg
    37741 gtggcaattc tgattatgct gccgactctc ggcatcggtg atatgaacaa tctctataaa
    37801 tggctgttga atcttaactc ggtagtgatg ccgctgcgtt atttatgggt atttgttgca
    37861 tttattgcag tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt tattcgtaat
    37921 aagcctctgg caatgaccgt cgggatttgg tgttttgcct ttaccgcctt tgcctgtttg
    37981 acggggatct tcccgaaaat ggaagccttc actgcagagt ggaccttcca gttggcgctg
    38041 aatgttgcaa cgccgtttgt gctggtagga ttaggactga ttttcccgct gctggcgcgt
    38101 aaagcgaata gtaaataatt attgtggtcg gtgacgctga ctctggcaga tctgacccgt
    38161 ttttacataa tcgagaaatg cgcgcaagcc gcaggacata tactggcggt ttggataata
    38221 gatctggaag cctggacgct ctgtgctcca gtctcccagt acacattcta aacgaccagt
    38281 atcaaggtac tccttgatct gttcatagag cagatacccg ataccagcac ccatcaaaac
    38341 ggcctccagt tcagcatcaa catcatccag aatgatattt cctgcgacag caatttccag
    38401 ctcctttgca aattgccagt ggaaaggttt gccgctggga taacgaaaaa caacacattg
    38461 atgattaagc agatcatgag gatggcgggg tttgccatat cttgcaaagt actctggcgt
    38521 tgctgctaca cataatttca ctggtgggcc gatcgccacg ctgatcatat ctttttcgac
    38581 gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga caagagaatc
    38641 gtcagttgtg agctcgactt taatatcagg atattcacgt gtgaaaccaa ccagtaaagg
    38701 cattaaaaat atacgtgcag ctacccgggc ggcattgatt ttgagcgttc ctgtcggtgt
    38761 cagacgaaaa tcattcatct catctaacat gatctgaatt tcgtcgaaag ctgggcgcaa
    38821 tcgttcatag agattagaac ccgcttccgt taacgatacg cttcgggttg tccgattgaa
    38881 taagcgaatt ttaagacgtt gttccagtgt tttaatacta tggctaatgg cggacgagga
    38941 taagcctaac tcatcgcccg ctgcacgaaa gctttgatta cgggcgacgg caaagaaagt
    39001 ggcaaagtca gacatattca tccgcattag tgaaaatcct tcattaactc atcctgaatc
    39061 atcacgttta caggattatg ccgcctgcgt aaagtgcgct ccagaactta acgtggaggt
    39121 aaaattatgc agtctgaacg tatttatttg gtatgggccc atcctcgtca tgattcattg
    39181 accgcaaata ttgctgatgc gatccatcag cgggcaatgg agcggaaaat acaggtgacg
    39241 gaactcgatt tatatcggcg taatttcaac ccagtgatga cgccggaaga tgaaccagac
    39301 tggaagaata tggataaacg ttattctcca gaggttcatc agctttattc agagctgctt
    39361 gaacatgaca cgttagtggt ggtttttcct ctctggtggt acagcttccc ggcaatgcta
    39421 aaaggatata ttgacagagt atggaataat gggctggctt atggagatgg gcacaaatta
    39481 ccattcaata aagttcgttg ggtggcgctg gttggaggag acaaagaatc atttgtccag
    39541 atgggctggg aaaaaaatat aagcgattat ttaaaaaata tgtgcagtta tcttggtatt
    39601 gaagatgccg atgtcacttt cttgtgtaat acagtggtat tcgatgggga agaacttcac
    39661 gcgagctatt atcagtcgtt attatctcag gtacgggata tggtagatgc actataagat
    39721 gtgttaaaaa cgctgtagca gaatgaagcg cggaataaaa aagcggcaac tcaataaagt
    39781 tgccgcttta cggggaaatt agaacattac cttatgaccg tactgctcaa gaatgccttt
    39841 cacgcgttcc atggtctctt tcttaggtgg tttaacaccg tcgagtttgt actcttcacc
    39901 cattgccacc catttgtgtt tgcccagctc gtggtagggg aggagttcga ttttctcaac
    39961 gttgcccata tcacgggtaa attcaccgag gcgatgcgct gaatcgtcat cgtcagacca
    40021 gcctgggaca acaacgtagc ggatccacac cttcacattt ttgttcgcca gatatttggc
    40081 gaactccagc gtgcggtggt tggaaactcc aaccagattt tggtggatct cgtcgttcat
    40141 ctgtttgaga tcgagcatta ccaggtcggt tacttccagc agttcatcaa tcaccggatc
    40201 gtaacgacga acaaaaccgt tggtgtccag acaggtatga atgccttctt ttttgcaggc
    40261 gcggaaccag tcacgaacaa actcagcttg taggattgcc tcaccgccgg atgcggtaac
    40321 gccgccgccg gaagcgttca taaagtggcg ataggtcacc acctctttca ttaaatcttc
    40381 aacggtaact tctttaccgc catgcgtatc ccaggtgtcg cggttatgac aatacaggca
    40441 gcgcatcagg cagccctgga aaaaggtgat aaagcgaata cctgggccgt ctacggttcc
    40501 acaggattca aaggagtgaa tgcgaccaat aactgacatt gcggtgtttc tccagatgtg
    40561 gcccatctga ggccgtgttg gtgcgcagct cgaaggctac gtcgagtctg ttttggcagt
    40621 caccttaaag tatagatagc tgacaaaaaa ggctctcgtg ctaaaaaagg ccccactttc
    40681 gtggagcctt tattgtacgc tttttactgt acgatttcag tcaaatctaa ttacatagat
    40741 tgagtgaagg tacgagtaat aacgtcctgc tgctgttctt tagtcagcga gttgaaacgt
    40801 actgcgtagc cagatacacg gatggtcagc tgcggatatt tttccgggtt ttccatcgcg
    40861 tcgagcagca tttcacggtt catcacgtta acgttcaggt gctgaccacc ttcgatggat
    40921 gcttcgtggt ggaagtaacc atccatcaga ccagccaggt tggtcttacg aacttcgtcg
    40981 tctttaccca gtgcgttcgg aacgatagag aaggtgtagg agataccatc tttagcgtaa
    41041 gcaaacggta gtttagcaac ggaagtcaga gacgctacag cacctttctg gtcacgaccg
    41101 tgcatcgggt tagcacccgg tccgaacggc gcgccagcac gacgaccgtc tggggtgtta
    41161 ccagttttct taccatacac aacgttagaa gtgatggtca gaacagactg agtcgggata
    41221 gcgtcacggt aggtgtgcag tttctgaatt ttcttcatga aacgttctac caggtcaaca
    41281 gccaggtcat ctacacgcgg atcattgtta ccaaactgcg ggtattcgcc ttcgatttcg
    41341 aagtcgatag ccagaccgtc ttcgtcacga atcggtttaa ctttcgcata tttgattgca
    41401 gacagggagt cagcagcaac ggacagacca gcgataccac acgccatggt gcggataacg
    41461 tcacggtcgt gcagcgccat cagagaggct tcgtagctgt acttgtcgtg catgtagtgg
    41521 atgatgttca gtgcagtgat gtactgttta gccagccagt ccatgaagtg atccatgcgc
    41581 tccatcactt catcatagtt caggacatcg cctttgatcg gttcagactt cggaccaacc
    41641 tgcattttca gtttttcgtc aacgccgccg ttgattgcgt acagcatggt tttcgccagg
    41701 tttgcacgcg caccgaagaa ctgcatttgt ttaccaacga tcatcgggct tacgcagcaa
    41761 gcaatagcgt agtcatcgtt gttgaagtcc ggacgcatca ggtcatcgtt ctcatactgc
    41821 agagaagagg tgtcgatgga cactttagcg gcgaatttct tgaagttcag cggcagtttt
    41881 tcagaccaca gaatggtcat gttcggttcc ggagacggac ccatggtgta cagggtgttc
    41941 aggaaacgga agctgttttt ggtaaccagg gtacgaccgt cgaggcccat accaccgata
    42001 gattcggttg cccagatcgg gtcgccagag aacagttcat cgtattccgg agtacgcagg
    42061 aagcgaacca tacgcagttt catgaccagg tggtcaacca tttcctgcgc ttcttgttcg
    42121 gtgatcttgc cagctttcag gtcacgttcg atgtacacat ccaggaaggt ggaggtacga
    42181 ccgaaggaca ttgcagcacc gttctgagac ttaacagcag ccaggtagcc gaagtaagtc
    42241 cactggatag cttcctgagc gttggtagcc ggaccagaga tgtcgtagcc gtatttcgca
    42301 gccatttctt tcatctgacc cagagcgcgg tgctgttcag cgatttcttc gcgcagacgg
    42361 atagtctgtt ccaggtttac gccgttttcc agatcagcct gcagagaagt gaactgtgcc
    42421 agtttgtctt tcatcaggta gtcgataccg tacagcgcaa cgcgacggta gtcaccgatg
    42481 atacggccac ggccatatgc atctggcaga ccggtcagaa caccagattt acggcaacgc
    42541 aggatgtccg gagtgtaaac gtcgaacacg ccctggttgt gagttttacg gtattcagtg
    42601 aagatttttt tgatcatcgg atccagttcg cggttgtacg ctttgcagga accttcgatc
    42661 attttgatac caccgaacgg gataagagca cgtttcagcg gagcttcagt ctgcagacca
    42721 acgattttct caagctgctt gttgatgtag ccagcgtcgt gagaggtgat ggtggaagca
    42781 acagcggtgt caaagtcaac tggcgcgtga gtgcggtttt ccagtttaac gccttccatt
    42841 actttgtccc acagggtggt ggtcgcttca gtagcgccag ccaggaagga ctcgtcaccc
    42901 tcgtacggag tgtagttttt ctgaatgaag tcacggacgt ttacttcatt ctgccagtca
    42961 cctttggtaa aaccttccca ggctgtggct aacttttcat taagctcgga catgtaacac
    43021 ctaccttctt aagtggattt tttatttact gcgtacttcg acaaccatta atggtgatcg
    43081 ttttcacgca ggtaaatgac ccagtatgtc aacccaacca acaaaccacc gccgataata
    43141 ttaccgatcg taaccggaat caggttatca gtgatgaagt tcatcacggt caggtgagaa
    43201 aaattttccg gtgcagaacc gacagcggtc cagaattccg gagatgcgaa gtcgcggatt
    43261 acaataccca tcgggatcat aaacatgttt gcgatactgt gctcaaaacc gctggcaaca
    43321 aacatcgcga ccggcagcac cataatgaac gctttgtcca tcaggctacg gccagaataa
    43381 ctcatccata ctgccagaca taccatcagg tttgccagga taccaaggca gacggcctca
    43441 ataaaagtat ggtgcacttt gtggtcggcg gtttgtagga cgtttagtcc ccattgacca
    43501 tttgcggtca tatactcgcc ggaaagccac attaaaagta caaacagcag tgcgccgacc
    43561 aggttgccaa aatagacatt tagccagttt ttcgccaact gaccccaggt gatgcgccca
    43621 ctcgccttag caacaacaat caacactgtg gaagtaaaga gatcggctcc gcagacaaca
    43681 caaagaatca gccccagaga gaagcaaatg ccgccaacca gttttgccat gccgaagggc
    43741 attgtgcctg tgccagtggt tgctgtgata tagaagacga atgcgattga gatgaaaaca
    43801 ccggcggtaa tcgccagata gaaagtctta agcggatgtt tcgttgcttt atagacaccc
    43861 gcctcttcgg ccactttggc cattgcagca ggaagtaaaa gatcaaaagg gttgtcagct
    43921 ttcacactaa ctctctcttt attaagtcgg cgacgagata ctaacaaagc attatagatg
    43981 agaaattgat atagatcata tctcgcctgg cttataggcc cgtaactcgc atgg
//
LOCUS       DAPPNJ010000009        43235 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000009 DAPPNJ010000000
VERSION     DAPPNJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.009"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            74..2713
                     /locus_tag="RPM73_000368"
     CDS             74..2713
                     /locus_tag="RPM73_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDX6518763.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPAYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2710..2895
                     /locus_tag="RPM73_000369"
     CDS             2710..2895
                     /locus_tag="RPM73_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDX6518764.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2900..3229
                     /locus_tag="RPM73_000370"
     CDS             2900..3229
                     /locus_tag="RPM73_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDX6518765.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3401..4306)
                     /gene="feaR"
                     /locus_tag="RPM73_000371"
     CDS             complement(3401..4306)
                     /gene="feaR"
                     /locus_tag="RPM73_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDX6518766.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4542..6041
                     /gene="feaB"
                     /locus_tag="RPM73_000372"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             4542..6041
                     /gene="feaB"
                     /locus_tag="RPM73_000372"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDX6518767.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(6099..8372)
                     /gene="tynA"
                     /locus_tag="RPM73_000373"
     CDS             complement(6099..8372)
                     /gene="tynA"
                     /locus_tag="RPM73_000373"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDX6518768.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNLNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8620..10665)
                     /gene="paaZ"
                     /locus_tag="RPM73_000374"
     CDS             complement(8620..10665)
                     /gene="paaZ"
                     /locus_tag="RPM73_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDX6518769.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            10950..11879
                     /gene="paaA"
                     /locus_tag="RPM73_000375"
     CDS             10950..11879
                     /gene="paaA"
                     /locus_tag="RPM73_000375"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDX6518770.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            11891..12178
                     /gene="paaB"
                     /locus_tag="RPM73_000376"
     CDS             11891..12178
                     /gene="paaB"
                     /locus_tag="RPM73_000376"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDX6518771.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12187..12933
                     /gene="paaC"
                     /locus_tag="RPM73_000377"
     CDS             12187..12933
                     /gene="paaC"
                     /locus_tag="RPM73_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDX6518772.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            12948..13445
                     /gene="paaD"
                     /locus_tag="RPM73_000378"
     CDS             12948..13445
                     /gene="paaD"
                     /locus_tag="RPM73_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDX6518773.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13453..14523
                     /gene="paaK"
                     /locus_tag="RPM73_000379"
     CDS             13453..14523
                     /gene="paaK"
                     /locus_tag="RPM73_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDX6518774.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14520..15287
                     /locus_tag="RPM73_000380"
     CDS             14520..15287
                     /locus_tag="RPM73_000380"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDX6518775.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15287..16075
                     /gene="paaB"
                     /locus_tag="RPM73_000381"
     CDS             15287..16075
                     /gene="paaB"
                     /locus_tag="RPM73_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDX6518776.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16077..17504
                     /gene="paaC"
                     /locus_tag="RPM73_000382"
     CDS             16077..17504
                     /gene="paaC"
                     /locus_tag="RPM73_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDX6518777.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17494..17916
                     /gene="paaI"
                     /locus_tag="RPM73_000383"
     CDS             17494..17916
                     /gene="paaI"
                     /locus_tag="RPM73_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDX6518778.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGDTITGEA"
     gene            17916..19121
                     /gene="paaJ"
                     /locus_tag="RPM73_000384"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17916..19121
                     /gene="paaJ"
                     /locus_tag="RPM73_000384"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDX6518779.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVI
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19148..20461
                     /gene="paaF"
                     /locus_tag="RPM73_000385"
     CDS             19148..20461
                     /gene="paaF"
                     /locus_tag="RPM73_000385"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDX6518780.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20562..21512
                     /gene="paaX"
                     /locus_tag="RPM73_000386"
     CDS             20562..21512
                     /gene="paaX"
                     /locus_tag="RPM73_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDX6518781.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     VFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            21494..22084
                     /gene="paaY"
                     /locus_tag="RPM73_000387"
     CDS             21494..22084
                     /gene="paaY"
                     /locus_tag="RPM73_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDX6518782.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKLTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            22314..23174
                     /gene="pdxI"
                     /locus_tag="RPM73_000388"
     CDS             22314..23174
                     /gene="pdxI"
                     /locus_tag="RPM73_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDX6518783.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23238..25544
                     /locus_tag="RPM73_000389"
     CDS             23238..25544
                     /locus_tag="RPM73_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDX6518784.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25715..26320
                     /locus_tag="RPM73_000390"
     CDS             25715..26320
                     /locus_tag="RPM73_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDX6518785.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            26320..27216
                     /locus_tag="RPM73_000391"
     CDS             26320..27216
                     /locus_tag="RPM73_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDX6518786.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTSQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            27232..28989
                     /locus_tag="RPM73_000392"
     CDS             27232..28989
                     /locus_tag="RPM73_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDX6518787.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            28979..30295
                     /locus_tag="RPM73_000393"
     CDS             28979..30295
                     /locus_tag="RPM73_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDX6518788.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(30346..30951)
                     /gene="azoR"
                     /locus_tag="RPM73_000394"
     CDS             complement(30346..30951)
                     /gene="azoR"
                     /locus_tag="RPM73_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDX6518789.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31152..35054
                     /gene="hrpA"
                     /locus_tag="RPM73_000395"
     CDS             31152..35054
                     /gene="hrpA"
                     /locus_tag="RPM73_000395"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDX6518790.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            35326..36126
                     /locus_tag="RPM73_000396"
     CDS             35326..36126
                     /locus_tag="RPM73_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDX6518791.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36323..37762
                     /gene="aldA"
                     /locus_tag="RPM73_000397"
     CDS             36323..37762
                     /gene="aldA"
                     /locus_tag="RPM73_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDX6518792.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37804..38805)
                     /gene="gap"
                     /locus_tag="RPM73_000398"
     CDS             complement(37804..38805)
                     /gene="gap"
                     /locus_tag="RPM73_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDX6518793.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            38994..39524
                     /gene="cybB"
                     /locus_tag="RPM73_000399"
     CDS             38994..39524
                     /gene="cybB"
                     /locus_tag="RPM73_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDX6518794.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39769..39942
                     /locus_tag="RPM73_000400"
     CDS             39769..39942
                     /locus_tag="RPM73_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518795.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(40014..40163)
                     /gene="hokB"
                     /locus_tag="RPM73_000401"
     CDS             complement(40014..40163)
                     /gene="hokB"
                     /locus_tag="RPM73_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDX6518796.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40562..42202
                     /gene="trg"
                     /locus_tag="RPM73_000402"
     CDS             40562..42202
                     /gene="trg"
                     /locus_tag="RPM73_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDX6518797.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42240..43163)
                     /locus_tag="RPM73_000403"
     CDS             complement(42240..43163)
                     /locus_tag="RPM73_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6518798.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT        10605 a        10239 c        11544 g        10847 t
ORIGIN      
        1 ctgaacggtt aaacatgcca caatacccgt attgaatgct taatttttcg ctaaatcagg
       61 atattaacta cccatgctgg gtaaatataa agccgttctc gcgctgttat tactgattat
      121 tcttgtgccg ttgacgctgc tgatgacgct cgggctgtgg gttcccacgc tggcgggcat
      181 ctggctaccg ctcgggacac gtattgcatt agatgaaagc ccacgcatta cgcgtaaagg
      241 tttaatcatt cccgatctcc gttatctggt gggagattgt cagcttgcgc atatcaccaa
      301 cgccagcctt tcacatccca gccgctggtt attgaacgtc ggcacggtag aacttgattc
      361 tgcttgtctg gcgaaattgc cgcagacgga gcaatcgcca gctgctccaa aaaccctcgc
      421 gcagtggcag tccatgctgc ctaacacctg gatcaatatc gataaactga ttttttctcc
      481 ctggcaggaa tggcagggaa aactctctct cgcattaacc tctgatatcc agcaactgcg
      541 ttatcagggc gaaaaagtta aatttcaagg ccagctgaaa gggcaacaac ttacagtcag
      601 cgaactggat gtcgtcgcgt ttgaaaatca gccgccggtt aaactggtgg gggaatttac
      661 tatgccgctc gtgccggatg gacttcctgt aagtgggcat gctactgcga cgttaaactt
      721 gccgcaggaa ccgtcactgg tggatgcaga gctggactgg caggaaaata gcgggcaatt
      781 gattgtgctg gcacgggata acggcgatcc gttgctcgat ttgccgtggc aaattactcg
      841 tcaacaattg accgtaagcg atggtcgctg gagttggccg tatgcaggtt ttcctttgag
      901 tggccgactg ggtgtcaaag tcgacaactg gcaggcaggg cttgagaacg ctctgatcag
      961 cggacgactg agtgtgctga cccaggggca agcgggtaaa ggcaacgcgg tgctaaattt
     1021 tggcccagga aaattaagca tggataacag tcagctgcct ctgcaactga ccggtgaagc
     1081 gaaacaggcg gacctcattt tatatgcccg tttacctgcg cagctaagcg gaagtctgac
     1141 tgacccaacg ttggcctttg agccaggcgc gttattacgt tcgaagggaa gagtcatcga
     1201 ttcgctggac atcgatgaaa tccgctggcc tttagcgggt gtaaaagtca cccaacgtgg
     1261 tgttgacgga cgtttgcagg ccatcttgca ggcgcatgaa aatgaactgg gcgatttcgt
     1321 gctgcatatg gatgggctgg cgaatgattt tctccctgac gctggccgct ggcagtggcg
     1381 ctactgggga aaaggcagtt ttacaccgat gaatgccacc tgggatgtcg caggaaaagg
     1441 tgagtggcat gacagcacga ttacgctgac cgatctctcc accggtttcg accagttaca
     1501 atacggtacg atgacggtag aaaagccgcg attaattctc gacaagcccg tcgtctgggt
     1561 acgtgacgca cagcatccct ccttcagcgg cgcgctgtca ctggacgccg ggcaaacgct
     1621 gttcactggc ggcagtgtgt taccgccatc aaccttaaaa tttagtgtcg atgggcgcga
     1681 tcctgcctat ttcctcttta aaggcgattt acatgctggt gagattggcc cggttcgggt
     1741 aaatggtcgc tgggacggta ttcggctgcg cggtaacgcc tggtggccta aacaatcact
     1801 gactgtattc cagccgctgg tgccacccga ctggaagatg aacttacgcg atggtgaact
     1861 gtatgctcag gttgcatttt ctgctgcgcc tgaacaagga ttccgcgcgg gagggcacgg
     1921 cgtgttgaaa ggcggtagtg cctggatgcc agataatcag gttaacggtg tcgattttgt
     1981 cctgcctttc cgttttgccg atggagcctg gcatctgggg actcgcggcc ccgttacgtt
     2041 gcgaattgcc gaagtgatta atctggtgac agcgaaaaat attacggctg atttgcaagg
     2101 gcgttatccg tggactgaag aagaaccctt gctgttgact gatgttagcg tcgatgtgtt
     2161 aggcggtaac gtactgatga aacaattacg tatgccgcaa catgacccgg cgctgttgcg
     2221 gctgaataat ctctcatcca gcgaactggt tagcgccgtc aatccgaaac aattcgccat
     2281 gtccggggca tttagtggtg cattgccatt atggctaaac aacgaaaaat ggatagtgaa
     2341 agatggttgg ctggcaaata gcgggccaat gaccttgcgg ctggataaag acaccgcaga
     2401 tgcggtggtg aaagacaata tgactgcggg ttcagcaatt aactggttgc gctatatgga
     2461 aattagccgg tcatcgacaa aaattaactt agataatctc ggtttattaa ccatgcaggc
     2521 caacattaca ggtaccagtc gcgttgatgg taaaagcggt acggtaaacc ttaattacca
     2581 tcatgaagag aatattttta cgctgtggcg cagtttacgc tttggcgata atctccaggc
     2641 atggttggag cagaacgcac gtctgccggg aaatgactgt ccgcaaggaa aagagtgtga
     2701 ggaaaaacaa tgaaaatttt actggctgcg ttgacgtcat cttttatgct ggttggctgt
     2761 acgcctcgca ttgaagtcgc tgcacctaag gaaccgatca ctatcaatat gaacgttaaa
     2821 attgagcatg agatcatcat caaggcagac aaagatgtcg aagagctgct tgaaactcgt
     2881 agcgatcttt tctgaggcga tgatgaagaa aacattactt ctttgtgcgt ttcttgttgg
     2941 gctggtaagc agcaatgtaa tggcattgac tctggatgaa gccagaactc aggggcgggt
     3001 aggtgaaaca ttttatggtt atctggttgc gctgaaaacg gatgctgaaa cagagaaatt
     3061 agtaaccgac attaatgccg aacgtaaagc gagttaccaa caactggcaa agcaaaataa
     3121 tgtgtcggta gatgatatcg cgaaactcgc cgggcaaaag ctcgtagctc gggccaaacc
     3181 gggggaatat gtacaaggga taaacggtaa atgggtgcga aaattttaat agcaaaaccg
     3241 caatatcggg ataacgctac aggagaaagc gattgatcta ttttcctgaa acaaggtgaa
     3301 tattcaaaaa ctcctgtcaa attgcctttc gccctgaaaa atgcatagtc aaatatctgt
     3361 tttaactaat tggcgttgca gtacacacaa cgccaattag ttagcggaat ttacgtcgat
     3421 actcgcctgg cgtcatccca aagcgttgct taaataccgt tgaaaaatga ctctggtcag
     3481 aaaatcccca atgaaagccg atgcctgcca gtttttcatc atctgcggca tggcgaatcg
     3541 catctgcaca aaaatcgaga cgacggttac gaatatattg cgcgactacc aaacctttat
     3601 cggcaaacat tcggtacaaa ctacgtactg acatacctgt ctctccggct atccactccg
     3661 ggcgtaatat ctcttcgcga atattatcgt ctatcaacgt aaccactttt tgaaactgac
     3721 gttcacgacg aggttgaaca gattcccgct gatgaagtac cgggcgcagc agacacacca
     3781 tcgcctgtag cgcagcttca ctttctgttt cagaaagtgc cggattattc atgctctcct
     3841 gtaacaggcg atgactgagt tgcaccatgg gtaagtcagc gtccagtctt tctgcgcaga
     3901 caggtttttg atggggaaaa tattgttcca gcagagtgcg tggcaaaagt aatgaaatct
     3961 gtttagaaga ctcctgccag taaagcgaac aggggcgtga ggcatcgagt aacgtaatat
     4021 cgccagcgcc aatctgcacc tgacgctcat cctgctccat tattgcctga ccactaagct
     4081 gaaaaacggt gtaaaaccag gcatcgtcgc tgccttttac ttcctgccag gtgcgggata
     4141 aattcacccc gctggttgtc acggtactca gctttagtcc tttggcaaaa tgcgtatcca
     4201 gtacacccgt gtaacgctca gtcagcaggt gtccggtaaa atttccgcat acctgattga
     4261 tttgggaaag ccattgctga aactcattat ccactgcggg gttcatggca cgttttcgct
     4321 ctgtgaaatg tatttttatt gttgcatttg tgttgcagta aacgaagcta atgagcctga
     4381 ctataggaaa taagtcttct caggcataga gacataagcg gttattgtca cgatttgcgg
     4441 agcttgtcac agctgacaaa gcgaatgtca cagcgaaaaa agtgactttt cttgtcgctg
     4501 cgtacactga aatcacactg ggtaaataat aaggaaaagt gatgacagag ccgcatgtag
     4561 cagtattaag ccaggtccaa cagtttctcg atcgtcaaca cggtctttat attgatggtc
     4621 gtccaggccc cgcacaaagt gaaaaacggt tggcgatctt tgatccggcc accgggcaag
     4681 aaattgcgtc tactgctgat gccaacgaag cggatgtaga taacgcagtc atgtctgcct
     4741 ggcgggcctt tgtctcgcgt cgctgggccg ggcgattacc cgcagagcgt gaacgtattc
     4801 tgctacgttt tgccgatctg gtggaacagc acagtgagga gctggcgcaa ctggaaaccc
     4861 tggagcaagg caagtcaatt gccatttccc gtgcttttga agtgggctgt acgctgaact
     4921 ggatgcgtta taccgccggg ttaacgacca aaatcgcggg taaaacgctg gacttgtcga
     4981 ttcccttacc ccagggggcg cgttatcagg cctggacgcg taaagagccg gttggcgtag
     5041 tggcgggaat tgtgccatgg aactttccgt tgatgattgg tatgtggaag gtgatgccag
     5101 cactggcagc aggctgttca atcgtgatta agccttcgga aaccacgcca ctgacgatgt
     5161 tgcgcgtggc ggaactggcc agcgaggctg gtatccctga tggcgttttt aatgtggtca
     5221 ccgggtcagg tgctgtatgc ggcgcggccc tgacgtcaca tcctcatgtt gcgaaaatca
     5281 gttttaccgg ttcaaccgcg acgggaaaag gtattgccag aactgctgct gatcacttaa
     5341 cgcgtgtaac gctggaactg ggcggtaaaa acccggcaat tgtattaaaa gatgctgatc
     5401 cgcaatgggt tattgaaggc ttgatgaccg gaagcttcct gaatcaaggg caagtatgcg
     5461 ccgccagttc gcgaatttat attgaagcgc cgttgtttga cacgctggtt agtggatttg
     5521 agcaggcggt aaaatcgttg caagtgggac cggggatgtc acctgttgca cagattaacc
     5581 ctttggtttc tcgtgcgcac tgcgacaaag tgtgttcatt cctcgacgat gcgcaggcac
     5641 agcaagcaga gctgattcgc gggtcgaatg gaccagccgg agaggggtat tatgttgcgc
     5701 caacgctggt ggtaaatccc gatgctaaat tgcgtttaac tcgtgaagag gtgtttggtc
     5761 cggtggtaaa cctggtgcga gtagcggatg gagaagaggc gttacaactg gcaaacgaca
     5821 cggaatatgg cttaactgcc agtgtctgga cgcaaaatct ctcccaggct ctggaatata
     5881 gcgatcgctt acaggcaggt acggtgtggg taaacagcca taccttaatt gacgctaact
     5941 taccgtttgg tgggatgaag cagtcaggaa cgggccgtga ttttggcccc gactggctgg
     6001 acggttggtg tgaaactaag tcggtgtgtg tacggtatta atctggttcg ctcataagta
     6061 aaaaacggca cctggtgccg tttttttgtc tgaaacaatc acttatcttt cttcagcgcc
     6121 cctagcgttg gcgtttcgtc aaagaagttc catggtttaa gcagagtatg tacccattcg
     6181 gtcggcataa tcggccactc ttcggcacgg gccacatgtg tggtgccggt ggtcatccag
     6241 ataacagcgt cggtgttgtc cagcgactcg ttatccttac tgtattgtcc aagaccggtg
     6301 tcatgagtgg aacggttcgg atatttgcct tccgggaaac gctcgccagg gtgataacgc
     6361 gttacccaga gctgcttgtc cataaagctt aaacgatgat agatccactc gtccggcgcg
     6421 aactgggcac cttttgctac cgggtgagta ccacctgcat aaggaataat ttgataggaa
     6481 actggattgc ccatgcgatt ctctttgttc aggttactca acagacgaat cgtgcccgga
     6541 tcaaacttct gcgcggcatc ctgttcattg ccgatgttgt actgattaac ttgcatggta
     6601 ctggtgcgtg ggccaccggc agtattcggt tttaccaccg ggtccatcgc caccaggctg
     6661 ttattctcgc catctacatc cagatcgagg cggaaattat aaatatgttg gtgtgtagta
     6721 cccacgatat tgtgatcgat aagcgtgccg tagcgcgtgt catctttcgc cgtctcatcg
     6781 tgcatggttt tcgctttaac acctttcacc gcttcgatgc ccgtagcacc ggcatcaatg
     6841 ccaatagtgc cgttttcatg gaagatccag tcaaaaatgt agtcatagtt acccactgta
     6901 ctgatccagc gcaccaccaa ctcccggcgt tcggtactga cgttgggctg gcccatttcc
     6961 tgatgcttat actcaggccc ggcataacgt tcaaataccg cgatagcgcg agggatctcc
     7021 atcggcacgc cagtgtagtc ggcgatggtt tcattaagga gcactgcgtt agacggggca
     7081 tctttaccac gagcaattgg tgaggttagc gtgcccatac cgtagtcacc agagtccaga
     7141 tacgctttaa agtaccagcc aatatcagga tcaccgtaag gcacaatcat gccgccgaga
     7201 gaaccttcgt acatgacttt gcgtttggtg ccattgtcgt tataagtcac ggtggagatc
     7261 ataggtccga cgcgcgagtt catgctgagg tgaaaatccc agttccgcca gtgaatcata
     7321 tcgccagtaa tggtgtaatt tttaccttca ggctcaatga tttgcatagg cttaactgcc
     7381 ggagcaacgc ggtcacggcc atcaaatggg cgtgcggtca ttggcaccgg aactaccgga
     7441 ccttcttcaa tcttaacgat ttttttctgt tctaaatcaa cgaccgccac caggttttcg
     7501 atgggatgtg cccagtagtt gccatcaccg acatcaagat agctgatgac tttgagcaac
     7561 cgggcatctt gtttcaggcc atctttacca tcgaaataac ctacggtcag cggcgtggta
     7621 atcactttct tcgcatcagt aataccgcgc ttcttcacgg cagcggcaaa ttcttcactg
     7681 ttgttaataa tgttctgcac actggcgaaa tcatccagca acaccatacc gtgggcgtct
     7741 ttaatgggtt gccaggagag cagtttgttg ttttgcagat ccaccaccgc ttcgatgata
     7801 tgtttgccgt cgagcataat gacgtcggct ttgcgtggct ggtcaaccgg tttgttttcc
     7861 agcgcaaacg cccagacagc ttctttatct ggcggtagca gggagatctc agtaaaacgg
     7921 gtattgggtt tgaagtccgc ggaagcttta acaatttcaa cggcctgttt aatttcgtcc
     7981 gcagttagcg cattaagtgg gtgagggcgc ttttctacct gaaaggtttg atccagcccg
     8041 gactggaaaa catcgttaat aaaggtgtca gaaacccagg ctttattgtc tttcatcact
     8101 accggtactt gcagtgccag aggctgacca ttaacaattg ctgtttgcgc accaggcttc
     8161 actttcacgt acgcgccatc tttaatcagg gtaaagagct gggcgtagtc gtcccactgc
     8221 acatcggcac caaattcttt aagcgtttta tccattggca ccatatgcgc ttcaccaccg
     8281 tgggcaaata ccggcgcttg ccaggcgaaa cttaaggcga ctgccaacgc cagggttgtt
     8341 ttacgggcag aatacagaga ggggcttccc attattaacc tcgtcagatg ttgtgttctt
     8401 gttagcaacc gcgctctgtg ggcggtttag tcaggttcac attatcagta ctgatgcaaa
     8461 ggggattgcc tgcacctgcc aggttgtttg gcaggtgtgc cagcttttca tacagtggat
     8521 gccctgaaaa tagatgtaca cattatgcat aatgtgacaa cgtcacaaaa tttagtgaaa
     8581 taaaagggca actattcgcc gttgcccttc attcaccgat taatcgacaa aatcaccgtg
     8641 ctgcctggcc accagcgtca gaattgaata cagcgccacc ggggtttgat gctgattgaa
     8701 tacctctacc gcccattcca ccacacctgt tggtttttct tctgcgctac gctgtttttt
     8761 cagcgtcttg cgcttacagg tgagacgcac ctggatggta tcgcctggtt ttacgggttc
     8821 gataaaacgc aagttttcca gcccgtagtt agctatgacc ggaccgacac cggcatcgac
     8881 aaacagaccc gcagccgcag aaagcacaaa atatccatgt accacacgct caccaaaaat
     8941 agattcggca gcagcaattt tatccatatg tgcatagaaa tgatcgccgc tgaggcaagc
     9001 aaagttaaca atatcggcct ctgtcattgt gcggcgggga gtcaacaggc tgtcgcctgg
     9061 ttgcagctcc tcaaaatatt tgcggaacgg atgaatacga tcttcttcga ctttcgcacc
     9121 gcgcacccac tgtttactga tagcggcaag catcgtcgga ctaccctgaa cagcggttcg
     9181 ctgcatgtaa tgtttcaccg ctcgtaaacc acctaattct tcaccgcctc ctgcgcgacc
     9241 aggcccacca tgtaccagtt gtggcagtgg ggagccatgc ccggtggatt cttttgccga
     9301 ctcttcattg aggatctgaa ttcgcccatg cgtacgtgcc gcgtctgcaa taaactgacg
     9361 cgcaatttgc ggatcagccg tcaccagcgt tcccgcaagg ctaccgccgc ctgcacaagc
     9421 cagttgcaga gcatgtcgct ggttttgtgc tggcatcagc gttgcgacag ggccaaaggc
     9481 ttctgttgca tgtaccgccg gtgcttcatc cggctgcgga cagtacaata aggttggcgg
     9541 gaagaaggca cccgcagcag ataaatccgc ctgaccaccg aggcgaatct cgcatcctgc
     9601 agccagcaat atgttcactt tttcctgcac atcggcacgc tgctcagcat ttaccagtgc
     9661 gcccattttc acgccttcct gagcaggatc accgaccacg actttctgta atcgcgcaac
     9721 cagagcatca ctgacagcat taaccaatgc ctgcggcaca ataatccgcc ggattgccgt
     9781 acatttttgc ccggcttttg tggtcatctc acgcacaact tcacgaataa acagcgcaaa
     9841 ctccggttga tccggggtga catcttcgcc cagtacgcag cagttcaggg aatcagcttc
     9901 catagtgaag gggatagatt tggcgacgat atttggctga actcgcagca tctgtccggt
     9961 cgccgctgac cccgtgaaag tcaccacatc ctggctgtcc agatgatcca acaagtcgcc
    10021 agcactaccg cagatcagac taattgcgcc ttcgggaaca agaccactat cgacaattga
    10081 tttcaccatc gcctgagtca gttgggccgt cgcggtagct ggtttgatga tggctggcat
    10141 tccgcccagc cacgttggtg ccagcttttc cagcattccc cagcagggga agttaaaggc
    10201 gttaatatgc actgccacgc ctgactttga ggtcagtaca tggcgcgcgg caaatccacc
    10261 ttctttcgat aaggggatca attcatcttc cggccacagc gtatcgtcag gcagctcccg
    10321 gctaccgagg ctggcgtaag taaataacgt cccaatgcca ccttcaatat caacccaact
    10381 gtctgcccgc gttgcgcctg tttgcgcaga aagagcatag aagcgctctt tttcactcag
    10441 cagatgttta gcgaccgctt taagcatcgc cgcacgttcg ataaaggtca tcgcgcggag
    10501 ggcgggggca cctttttcaa tggcaaactg gcgggcagcc gccatatcaa gaccttcact
    10561 ggtcacttcc cataacgcct cgccgctaat agcgtggtga atcaaacggc tacggccccg
    10621 gccagactgc caggtaccgg ataagaaact ggctaactgc tgcatcgcta ctctccagat
    10681 gtttcacatt tctgttgcta atagttaaat cgcgaatcat aaaaagcaag gggtctttta
    10741 acgaaatgtt aactatgcga tctgtatagc aactgcggaa aacattaacg cactgataaa
    10801 taatgattta taaaaatagg gtgcgaaatc cgtcacagtt caagcataca aaatttgtga
    10861 ttttagttaa ctattgtgta actttcataa aacaatgtga ttcgtgtttt taattaattc
    10921 acgaaaactg gaatcgtaaa ggtgatgacg tgacccaaga agaacgcttt gagcaacgga
    10981 tagcccagga aacggctatc gagccacagg actggatgcc cgatgcttac cgaaagacac
    11041 tgatccgcca gattgggcag catgcgcact ccgagattgt tggtatgttg cctgaaggta
    11101 actggatcac tcgcgcacca accttgcggc gtaaagccat tctgttggcc aaagtgcagg
    11161 atgaagccgg tcatggtctg tatctctata gcgccgccga aacactgggc tgcgccagag
    11221 aagacatcta ccaaaaaatg ctcgacggac ggatgaaata ttcctccatc tttaattatc
    11281 cgacattgag ttgggctgat atcggcgtta tcggctggct ggtggatggc gcagcgatcg
    11341 tcaatcaggt ggcgttatgt cgcacttctt atggcccgta tgccagagca atggtgaaaa
    11401 tctgcaaaga agagagtttt caccagcgtc aggggtttga agcctgcatg gcactcgcgc
    11461 agggcagcga agctcaaaag cagatgttgc aagacgccat taaccgtttc tggtggccag
    11521 ccttaatgat gttcgggcca aacgatgata actcgccaaa cagcgccaga agtctcgcct
    11581 ggaaaatcaa acgtttcacc aacgacgaac tccgccagcg tttcgtggac aacaccgttc
    11641 cacaggttga aatgctcggt atgaccgttc ctgacccgga tctgcatttt gacactgaaa
    11701 gcggtcacta ccgctttggt gagatcgact ggcaggagtt taacgaagta attaacggtc
    11761 gcggaatttg taatcaggag cggctcgacg ccaaacgtaa agcctgggaa gaaggtacct
    11821 gggtacggga agcagcgctg gcccatgcac aaaaacaact tgcccgtaag gtcgcataag
    11881 gagattcaaa atgagtaatg tttactggcc gttatacgaa gttttcgtgc gtggcaaaca
    11941 gggcttatca caccgccatg ttggcagttt acatgctgcc gatgagcgga tggcactgga
    12001 aaatgcccgt gatgcttaca cccgtcgtag cgaaggatgt tcaatttggg tggtgaaggc
    12061 gagtgaaatt gttgcctcgc aaccggaaga acgcggtgaa ttttttgatc cggctgaaag
    12121 caaggtctat cgccatccaa cgttttacac catccctgat ggcattgagc acatgtgagg
    12181 tcggaaatga atcagttaac ggcttacacc ttgcgcctgg gcgataactg tctggtgctc
    12241 tcccagcggt tgggggaatg gtgcggtcac gcaccggaac tggaaatcga tctcgcactg
    12301 gcaaacattg gcctcgattt attaggtcag gcacgtaact tcttatcgta tgccgctgaa
    12361 ttagcgggag aaggtgatga agataccctg gccttcaccc gagacgagcg ccagttcagc
    12421 aacttattgt tggttgaaca accaaacggc aattttgccg acaccattgc acgccagtat
    12481 ttcatcgatg catggcatgt ggcgctcttt acccgtctga tggaaagccg tgatccgcaa
    12541 ctggcggcga tttctgccaa agcaattaaa gaagcgcgtt atcacctgcg ttttagtcgt
    12601 ggctggctgg agcgactggg caatggtact gacgtatcag ggcaaaagat gcagcaggcc
    12661 atcgacaagt tgtggcgttt tactgccgaa ctgttcgatg ccgacgagat tgatattgca
    12721 ctgagtgaag agggtattgc ggttgatcca cgcactttac gcgcagcgtg ggaagccgaa
    12781 gtttttgccg gaatcaacga agccacattg aacgtaccgc aagagcaggc gtatcgcact
    12841 ggcggtaaaa aaggactgca taccgaacac cttggaccca tgctggcaga aatgcagtat
    12901 ctccagcgtg tcttgcccgg tcagcaatgg taacagagga gatgggtatg caacgtctgg
    12961 ccaccattgc accgccacag gttcatgaga tatgggcgct actcagccag atcccggacc
    13021 cggagatccc ggtgctgacc attaccgatt taggcatggt gcgtaatgtg acacagatgg
    13081 gagaaggatg ggtgatcggc tttacgccga cttattccgg ttgtccggca acggaacatt
    13141 tgattggcgc gatacgtgag gcaatgacaa cccacgggtt tacccccgtt caggttgtgc
    13201 tgcaactcga cccggcatgg accaccgact ggatgacccc cgatgcccgt gagcgtctgc
    13261 gacagtatgg cattagcccg cccgccggac acagttgcca tgcccatttg ccgccagaag
    13321 tacgttgccc gcgctgcgcc agcgtccata ccacacttat cagtgaattt ggttccacgg
    13381 cctgcaaagc attgtatcgc tgcgatagtt gccgcgaacc tttcgattat ttcaaatgta
    13441 tttgaggatg ccatgacaac gtttcattcc ttaacggtgg caaaagtgga gccggaaacc
    13501 cgtgatgcgg tgaccattac ctttgcggtg ccccagcctt tgcaggaggc gtatcgcttt
    13561 cgccccggtc aacatttgac cttaaaagcc agctttgatg gtgaagaatt acgccgttgt
    13621 tactccattt gccgcagcta tctgcctggc gaaattagtg tggcggtgaa agccattgaa
    13681 ggcggacgtt tctcccgcta tgcccgcgaa cacatccgcc agggtatgac gctggaggtc
    13741 atggtgccgc aggggcattt cggctatcag ccacaggccg aacgccaggg ccgctatctg
    13801 gctattgcag caggatcagg tattacgcca atgctggcga ttatcgccac cactttacaa
    13861 accgagcctg aaagtcagtt caccctgatc tacggtaacc gtaccagcca gagcatgatg
    13921 tttcgccagg cactggcaga cctgaaagac aaatatcctc agcgtttaca gttgttgtgc
    13981 attttcagtc aggaaaccct cgacagcgat ctgcttcacg ggcgtattga cggtgaaaaa
    14041 ttacagtcac ttggggcctc gctcattaat tttcgtcttt atgatgaggc atttatttgt
    14101 ggtccggcgg cgatgatgga tgagacggaa gccgccttaa aagcactggg aatgccagat
    14161 aaaaccattc atctggagcg gtttaatacg cctggcacgc gcgtcaaacg tagcgttaac
    14221 gtgcaaagtg acggacaaaa agtgactgta cgtcaggatg ggcgggatcg ggaaatcgtg
    14281 cttaatgccg acgatgaaag cattctcgat gcggcattgc gccagggggc tgatctgccc
    14341 tatgcctgca aaggcggcgt ctgtgcgacc tgcaaatgca aagtgctgcg tggcaaagtg
    14401 gcgatggaaa ccaattacag tctggaaccg gatgaactgg ccgcaggcta tgtgttgagt
    14461 tgccaggcac tgccgctgac cagcgatgtg gtggttgact ttgacgcgaa ggggatggca
    14521 tgagcgaact gatcgtcagc cgtcagcaac aagtattgtt gctgaccctt aaccgtcccg
    14581 ccgcacgtaa tgcgctaaat aatgcccttc tgacgcaact ggtaaatgaa ctggaagctg
    14641 cggctaccga tagcagcatt tcggtctgtg tgattaccgg taatgcacgc ttttttgccg
    14701 ctggggccga tctcaacgaa atggcagaaa aagatctcgc ggccacctta aacgatacac
    14761 gcccgcagct atgggcgcga ttgcaggcct tcaacaaacc tctcatcgca gccgtcaacg
    14821 gttacgcgct tggtgcgggt tgcgaactgg cattgttgtg cgatgtggtg gttgccggag
    14881 agaacgcgcg ttttggtttg ccggaaatca ctctcggcat catgccaggc gcaggaggaa
    14941 cgcaacgttt aatccgtagt gtcggtaaat cgttagccag caaaatggtg ctgagcggag
    15001 aaagtatcac cgctcagcaa gcacagcagg ccgggctggt tagcgacgtc ttccccagcg
    15061 atttaaccct cgaatacgcc ttacagctgg cgtcgaaaat ggcacgtcac tcgccgctgg
    15121 ccttacaagc ggcaaagcaa gcgctgcgcc agtcgcagga agtggctttg caagccggac
    15181 ttgcccagga gcgacagtta ttcaccttgc tggcggcaac agaagatcgt catgaaggca
    15241 tctccgcttt cttacaaaaa cgcacgcccg actttaaagg acgctaatga tggaattcat
    15301 cctcagtcat gtagaaaagg gcgtgatgac actaacgctc aaccgcccgg aacgcctgaa
    15361 cagttttaat gatgagatgc acgcacaact ggcagagtgc ctgaaacagg tcgagcgcga
    15421 cgacactatc cgttgcctgt tacttacagg tgccgggcgc ggattttgtg ctggtcagga
    15481 tcttaacgat cgtaacgttg atcccacagg ccccgcaccg gatttaggga tgtcagttga
    15541 acgtttctat aacccactgg tacgtcgcct ggcaaaactg ccaaaaccgg tgatctgtgc
    15601 agtcaatggc gtggcggcag gggcaggcgc aacactggca ctggggtgcg acatcgttat
    15661 tgctgcccgt tcagcaaaat tcgtcatggc gtttagtaag ttaggcttaa tacccgattg
    15721 cggtggaacc tggttactgc cacgcgttgc cggacgagcg cgcgccatgg ggctggcact
    15781 gctggggaat caactgagtg ctgaacaggc gcacgaatgg gggatgatct ggcaggttgt
    15841 tgatgatgaa acgctggcag ataccgcgca acagctggca cggcatctgg cgacacaacc
    15901 gacatttggt cttggactta tcaagcaagc gataaatagc gctgaaacca atacgctcga
    15961 tacgcaactg gatctggaac gtgactatca gcgacttgcc ggacggagcg cagattatcg
    16021 tgaaggtgtc agtgcgttcc tggctaaacg ctcaccgcag ttcacgggga aatagcatga
    16081 tgataaatgt gcaaactgtg gcagtgattg ggagcggcac catgggggca ggcattgctg
    16141 aagttgccgc cagtcatgga catcaggttt tactgtatga catttctgct gaagcgctga
    16201 cccgcgcaat cgacgggata cacgcgcggc taaattcacg cgtgacgcgg ggaaaactga
    16261 ccgctgaaac ctgtgaacgc acattgaaac gcctgatccc ggtgaccgat attcacgcgc
    16321 tggcagcagc gaatctggtt attgaagcgg cgtctgaacg tctggaagtt aaaaaagcgc
    16381 tctttgcaca actggcggaa gtttgcccgc cacaaacgct attgaccact aacacttcgt
    16441 caatctctat aaccgcgatt gctgcggagg taaaaaatcc tgaacgtgtt gcggggctgc
    16501 atttttttaa cccggcaccg gtgatgaagt tggtggaggt ggtcagtggg ctggcaacgg
    16561 cagcggaagt tgtcgagcag ttgtgtgaac tgacattgag ttggggtaag cagccagtgc
    16621 gctgtcattc gacccccgga tttatcgtta accgtgttgc gcgtccttat tattccgagg
    16681 cctggcgggc actggaagag caggttgctg caccagaagt gattgacgct gcacttcgcg
    16741 atggcgctgg tttcccgatg gggccgctgg aattaaccga tctgattggt caggacgtca
    16801 attttgctgt cacctgttcg gtgtttaacg ctttctggca ggagcgtcgt tttttacctt
    16861 cgctggtgca acaggaactg gtgattggtg gacggttggg caagaaaagt gggctgggcg
    16921 tgtacgactg gcgcgcggaa cgtgaggcag ttgttggcct ggaagcggta agcgacagtt
    16981 ttagcccaat gaaagtagaa aagaaaagtg acggtgtcac ggaaattgac gatgttttat
    17041 tgattgagac acaaggcgag acggcacagg cgctggcaat acgactggca cgcccggtgg
    17101 tagtggtcga caaaatggcg ggcaaggtgg tgaccattgc tgcggcagcg gtgaacccgg
    17161 actcagcgac ccgcaaggcc atttattacc tgcaacagca gggcaaaaca gtgctgcaaa
    17221 ttgcagatta cccaggaatg ctgatttggc gaacggtagc aatgatcatc aatgaagccc
    17281 ttgatgcgct tcaaaaaggc gtggcctctg aacaggatat cgataccgcc atgcgtcttg
    17341 gggtgaatta tccatatggc ccacttgcct ggggagcgca acttggctgg cagcgaatat
    17401 taaggctcct tgaaaatcta cagcatcact atggcgaaga acgctatcgc ccatgttcat
    17461 tgctgcgcca acgggcgctt ctggagagcg gttatgagtc ataaggcctg gcaaaatgcc
    17521 catgcaatgt atgagaacga tgcctgcgcc aaagcgcttg gcatcgacat tatctcaatg
    17581 gatgaaggct ttgctgtagt gaccatgacc gtcactgcac aaatgcttaa cggtcatcaa
    17641 agttgccacg gcgggcagct attttcactg gctgatactg cctttgccta cgcctgcaat
    17701 agccaggggc tggcagccgt cgcttctgcc tgcacgattg attttttacg tccaggcttt
    17761 gccggagaca ccttaactgc tactgcgcag gtacgtcatc agggcaagca aaccggtgtt
    17821 tacgacatcg aaattgttaa ccaacaacaa aaaacggttg cgctgtttcg cggtaaatct
    17881 caccgcatcg gcgacaccat tacaggagaa gcctgatgcg tgaagccttt atctgtgacg
    17941 gaattcgtac gccaattggt cgctacggcg gggcattatc aggtgttcgg gctgacgatc
    18001 tggctgctat ccctttgcgg gagctgctgg tgcgaaaccc gcgtctcgat gcggagtgta
    18061 tcgatgatgt gatcctcggc tgtgctaatc aggcgggaga agataaccgt aacgtagccc
    18121 ggatggcgac tttactggcg gggctgccgc agagtgtttc cggcacaacc attaaccgct
    18181 tgtgtggttc cgggctggac gcactggggt tcgccgcacg ggcgattaaa gcgggcgatg
    18241 gcgatttgct gatcgccggt ggcgtggagt caatgtctcg ggcaccgttt gttatgggca
    18301 aggcaaccag tgcattttct cgtcaggctg agatgtttga taccactatt ggctggcgat
    18361 ttgtgaaccc gctcatggct caacaatttg gaactgacag catgccggaa acggcagaga
    18421 atgtagctga actgttaaaa atctcgcgag aagatcaaga tagttttgcg ctacgcagtc
    18481 agcaacgtac ggcaaaagcg caatcctcag gcattctggc tgaggagatt gttccggttg
    18541 tgttgaaaaa caagaaaggc gttgtaacag aaatacaaca tgatgagcat ctgcgcccgg
    18601 aaacgacgct ggaacagtta cgtgggttaa aagcaccatt tcgtgccaat ggggtgatta
    18661 ccgcaggcaa tgcctccggg gtgaatgacg gagccgctgc gttgattatt gccagtgaac
    18721 agatggcagc agcgcaagga ctgacgccgc gagcgcgtat cgtagccatg gcaaccgccg
    18781 gggtggaacc gcgcctgatg gggcttggtc cggtgcctgc aactcgccgg gtgctggaac
    18841 gtgcggggct gagtattcac gatatggacg tgattgaact gaacgaagcg ttcgcggccc
    18901 aggcattggg tgtaatacgc gaattggggc tgcctgatga tgccccacat gttaacccca
    18961 acggaggcgc tatcgcctta ggccatccgt tgggaatgag tggtgcccgc ctggcactgg
    19021 ctgccagcca tgagctgcat cggcgtaacg gtcgttacgc attgtgcacc atgtgcatcg
    19081 gtgtcggtca gggcatcgcc atgattctgg agcgtgtttg agcatatcaa cctgcgagta
    19141 ccctacaatg ataaccaata caaagcttga cccgattgaa accgcgtctg ttgatgagtt
    19201 acaggcgttg caaacacagc gtctgaaatg gacgctcaaa cacgcgtatg aaaatgtgcc
    19261 gatgtatcgg cgcaaattcg acgcagcagg cgtacatcct gatgatttca gggaactttc
    19321 agacctgcgt aaatttccct gtaccaccaa acaggatctg cgcgacaact atcctttcga
    19381 cacctttgcc gtgccgatgg aacaagtggt gcgcattcat gcttcttcgg gaaccacagg
    19441 taaaccgaca gttgtcggtt atacgcaaaa cgatattgat aactgggcca atattgtagc
    19501 gcgttctttg cgtgcggcag ggggctcgcc caaagacaaa attcatgttg cctatggtta
    19561 cggcctgttt actggtgggc tgggcgcgca ctatggtgcc gaacgtctgg gcgctacggt
    19621 gatcccgatg tctggcgggc agacggaaaa acaagcgcaa ctgatccgcg attttcaacc
    19681 agatatgatc atggttacgc catcttattg ccttaacctg attgaagagc tggagcggca
    19741 gttgggcggt gatgccagcg gttgctcgct gcgggttggc gtgtttggtg ctgagccgtg
    19801 gacacaggcc atgcgtaaag agattgaacg gcgtctgggg atcaccgcac tggatattta
    19861 tggcctgtca gaagtgatgg ggccgggggt ggcgatggag tgtctggaaa ctaccgacgg
    19921 tccaactatt tgggaagatc atttctatcc tgaaattgtt aatcctcatg acggcacacc
    19981 gcttgccgat ggtgaacatg gcgaactgtt attcaccacg ctgaccaaag aagcattgcc
    20041 ggtcattcgt taccgcacgc gtgatttaac ccgactgtta ccaggaacgg cgcggactat
    20101 gcgccgcatg gatcgcatca gcggacgcag cgatgacatg ttgattattc gcggtgtaaa
    20161 tgtctttccg tcacaactgg aagaagagat tgtcaaattc gaacatttat cgccgcatta
    20221 ccaactggag gtgaaccgcc gggggcatct tgattcactt tctgtgaaag tggagttgaa
    20281 agaaagtagc ctgacattga cgcatgagca gcgttgccag gtatgccacc agctgcgcca
    20341 tcggattaag tcgatggtgg ggatctctac cgatgtgatg atcgttaact gtggcagtat
    20401 cccgcgttca gaaggcaagg cgtgtcgggt gtttgatctg cgcaatattg ttggtgcctg
    20461 acgtgtcgtc tggccctggt gaggtaaagc gtcagggcca gaagtcgata cgacctgtgc
    20521 tatgattcat aaatcacaac aataacaaca gactgaatcg aatgagtaaa cttgatactt
    20581 ttatccaaca tgctgtaaac gctgttccgg tcagtggcac atctttgatc tcctctctgt
    20641 atggtgattc gctttcccat cgtggtggtg aaatctggct tggtagtctg gctgctttgc
    20701 tggaagggct gggatttggt gagcgtttcg tccgcactgc tttgtttcgt cttaataaag
    20761 aaggctggct ggatgtttcc cgcatcgggc gacgcagttt ctatagcctc agtgataaag
    20821 gcttgcgcct gacgcgacgg gcagaaagta aaatttatcg cgcagagcaa cctgcatggg
    20881 atggtaaatg gctcctgttg ctctcggaag gtttagataa agcgacgctg gctgatgtca
    20941 aaaagcagtt gatctggcaa ggttttggcg cactggcacc cagcctaatg gcatcgccgt
    21001 cgcaaaaact ggcggatgta cagacacttt tgcatgaagc gggggtggcg gataacgtga
    21061 tttgttttga agcgcaaata ccactggcgc tttctcgcgc agcactgcgt gccagagtag
    21121 aagagtgctg gcatttaact gaacaaaatg ccatgtacga aacctttatt cagtcattcc
    21181 gcccgctggt gccgctgtta aaagagacgg cagacgagtt aaccccggag cgtgcatttc
    21241 atattcagct tttactgatc catttttatc gccgtgtcgt ccttaaagac ccattgttgc
    21301 cggaggagtt gcttccggct cactgggcag ggcatacggc gcgtcagctg tgtatcaaca
    21361 tttatcagcg cgtagcgcct gctgctttag tgttcgttag tgaaaaaggt gaaacctcag
    21421 tcggtgaact gcctgcgccg ggaagcctgt attttcaacg ttttggcggc ttgaatattg
    21481 aacaggaggc gttatgccaa tttaccagat agatggtctg actccggttg tgccagaaga
    21541 gagctttgtc catccgacag cggtattgat cggcgatgtt attctcggca agggcgttta
    21601 cgttgggcct aatgccagcc tgcgtggcga ttttggtcgt atcgtggtga aagatggcgc
    21661 gaacattcag gataattgcg ttatgcacgg ttttcccgag caggatactg tcgtggaaga
    21721 agatgggcat attggtcata gcgctatcct tcacggctgc attatccgcc gcaatgcatt
    21781 agtgggaatg aacgcggtag tgatggacgg tgcggtgatt ggcgagaaca gcattgttgg
    21841 cgcatccgca tttgtgaaag ccaaagcaga aatgcctgct aattacctga ttgtcggcag
    21901 cccggccaaa gcgattcgtg aacttagtga gcaggagttg gcatggaaaa agcagggtac
    21961 gcatgagtac caggtgctgg tgacacgctg taagctgacg ttgcatcaag tcgaaccatt
    22021 gcgggaagtt gaacctggca ggcagcgcct ggtatttgat gagaatctgc gaccgaaaca
    22081 gtaacgtcca gatactgaac attctaattt ttagcatgtg atacctaaac cgcaacgctg
    22141 atacaggttg cggtttttta ttgccggatg cggtacttgt cgcgttttgt tttgtgtttt
    22201 tcaggacaat agagcaactc atccagtaat cttgtttacg ccctcgcatt atttatctct
    22261 tttcgtttct atactgattt ttcttaatcc gttttattac agggcagggt gcgatgagca
    22321 gcaatacatt tactctcggt acaaaatccg ttaaccgtct tggttatggc gcgatgcaac
    22381 tggcaggtcc tggggttttt ggccccccaa gagatcgcca cgtcgctata accgtgctgc
    22441 gcgaggcgct ggcattgggc gtcaatcaca ttgataccag cgacttttat ggtccgcacg
    22501 tcaccaatca gattatccgc gaagcgcttt atccttactc tgacgacctg acaattgtca
    22561 ctaaaattgg tgcgcggcgt ggagaggacg cttcctggtt gcccgccttt tctccggcag
    22621 agttgcaaaa agcggtgcac gataatctac gtaatctcgg cctggacgta ctggatgtgg
    22681 ttaacctgcg cgttattatg ggggatggtc atggcccagc ggaaggatcg attgaggcca
    22741 gcctgaccgt gctggcagag atgcaacaac aaggcttggt aaaacatatt ggcctgagca
    22801 acgtcacacc gacgcaggtt gcagaggcgc gcaagattgc cgaaattgtc tgtgtgcaaa
    22861 acgaatacaa catcgcgcac cgtgctgatg atgcaatgat tgatgctttg gcccacgatg
    22921 gcattgccta cgtgccgttc ttcccgctcg ggggctttac accgctgcaa tcctctacgc
    22981 tgtcggatgt tgctgcgagc ctgggtgcaa cacccatgca ggtggcgctg gcgtggctgt
    23041 tacaacgttc accgaatatt ttgctgatcc cagggacgtc ttctgttgcg catttacggg
    23101 agaatatggc tgctgaaaaa ttgcatcttt ctgaggaagt gttgtctacg ttggatggta
    23161 tttcgcgaga ataacgaata tacaaaaggg aaagatgcat ttcccttttg tttctttttt
    23221 aatggcatgg agtgcatatg tttaaggcca ggatttgcgg ctggataggt ttacttcccc
    23281 tgtttatgct gagtcttcca gtacaggcgg aacttcgatg tgttgcaaat acagttgata
    23341 ttgaaccatt tttttctgcg gctaccgctg aagataaaca acaagttgaa caagctatca
    23401 acagtagcgt gaatcttgtc cccttcggtt tatctgcatc ggactggaaa gtgcatcgtg
    23461 gcgatttagt ggtagaaggt aatatagaga gtaatcaaaa attgattgtt cttggtaatt
    23521 tgacagtcaa aggtaatatt tccactttct ctctttctaa tccatgggtt attctcggta
    23581 acgtgacggc aacgaatata gttacggatt caccactgtt aattaccggt tcgataaatg
    23641 caagtggact ggtatttatc gactcatatt acgataatcc gtcttcgatt aaggggagta
    23701 ttaatgcgcg tggaatattt atcaatgaca taattgcacc tgttgttgcg tcttcgacaa
    23761 atagtgaatt catggtccgt gcgagtgaca aaaatgacac tgaaaatgtc aaaaaagcgc
    23821 tgatgataat aaatcctgat gcatattatt ggggtctaat aaatgatgaa gatgctctga
    23881 aagaaatttt taagcgaagc aatattcgca tggcagggaa tgtctgtaat cagatgaaaa
    23941 aagaagcgct gtttcgccct aagccttctc ctgagttagt gcaggaattg caaatgctgg
    24001 atgaaggcaa cgttgctgca tttgaaggac gagatattgc gacatttgat cttgccataa
    24061 tgcgaactct cccaaggctt aaaggaattt cagctaacct acgcaaacaa cttattaata
    24121 gcaatgatga acaaacgatt gaaagtatgg cgagatatat gcctgacaat gagattctgg
    24181 agttgaccga tcagcaattg ggttaccagc ctgttgttct ggggttgctc gaccgtgaac
    24241 cgctctctgt cgaaataatg acgcgaatga gtcgtctacc tgatggtgtc ggtccgttga
    24301 atcttgcgct acgtgaaaat ctccctctgg atatcgtaat gactctggcg aaaagagatt
    24361 gggatatgat cattcaggag ctttataaag atgcgtggtt attgcctgaa tccattattg
    24421 atggctatat ccgcagtgat gattcctcta ttcgtcaggt cggtgctgga ggacaactca
    24481 cctacaatca ggcaatgcag ttggcgaacg attcatcaaa caatgttgtc acaagcttag
    24541 cgttcaagct ggcagagatg aaacaccatg gtcaattatt gcggatgacg ccacaagaga
    24601 gtgacaaagt tgcagcctat ttataccaaa aattcgagaa tgacgatgat ctaatacgtg
    24661 tgttattttt agcattgcca gataacttac agtttaattt tgttaaaagg atggagaaaa
    24721 aatcccctgc ctacttttgc tgtcgggata tgcaggtaat tcactctgac gctgctctac
    24781 aacggttatt gacacgtttc aacgatcctg aggggtggag taatctggcg aaaaatcagt
    24841 atctgagcac gtcgatgaaa caaaaaattt ggcaacgtgc tttatcgcat cggaaaaata
    24901 atccgaaagc agattcagct gcgtatgaaa ccagcgctga tatgattttg tctgagctta
    24961 tcagccacgg tgaagttgat gatcagatgc tattaaatgc cacagccctg atacgtttgg
    25021 aagactggga ctttttagag agtgcattag ttagttggga taatttaccc gctgttgtcc
    25081 tcaaggaatt acagcaaaat acgccacgca atgatatttg ggcgaagttt tttctgaggc
    25141 aggaaaacag ctcccgtgcg caggttgatg aagcattacg cgtctattat gcactcgacc
    25201 ccgatgcgtt agcacaactg gatgtactgg caaaacaacc ggatcgtata tggtggagta
    25261 cactggcgaa aagcaatctc acatttttca agttcggcgc acttaacaac cgccacacac
    25321 cgcctgcagt actggcggca gaaattgatc ccgagtggtg gattgtggcg atgaataatc
    25381 cccgttttcc agttgatgta ttaaaggcga ggctgaaacg tgatcctttg ctggcgttag
    25441 agcttgttaa ccctgaactg gatttagtcc gccagctggc gcttaacggt aagacgcgcg
    25501 caatacggga acaagcgatg agaaaacttg atgagttgta ttgatccaca acgtaatatt
    25561 tcttaaccct ttgattaatt ggggataatt attcctgaca gtgagtcccc aataccttga
    25621 tatattctga atttttaatg aagcggcgtg ttgcgatatc tccgtcaggg gaattgatgc
    25681 accatagcgc aaaccgaatt atcaaggatt gataatgacg ctctaccaga taaaaccgct
    25741 ctttcagtcg ctgttaaggc cgacgatgtt ttggctttac aagcaccacg ttacagcgaa
    25801 tcacatcact cttgctgcac tggcgctttc tcttcttacc ggattgctgt tggtgttggt
    25861 ggcacaaccc atcctctttt tgctattgcc catcgtgctt tttatccgta tggcgctcaa
    25921 tgcgctggat ggcatgttgg cgcgtgagtg caatcagcaa acacgtctgg gggcgatttt
    25981 gaatgaaact ggcgatgtta tttccgatat tgcgctctat ttaccgtttt tatttttacc
    26041 ggaaagtaac gcatcactcg taatactcat gttattttgc accatattga ctgagttttg
    26101 tggtttactc gcgcagacaa ttaacggtat tcgcagttat gccggtccat tcggcaaaag
    26161 cgatcgtgcg ttaatatttg ggttgtgggg gctggccgtt gccatttatc cacagtggat
    26221 gcagtggaat aatcttttat ggagcattgc ttcaattctg cttctctgga ctgcgattaa
    26281 tcgttgtcgg agtgtgcttt ttatgagtgc tgaaagataa tgctggaaaa atctctggca
    26341 acacttttcg cgttgttaat tttagcaacg ctgataaacc gttttctgct atggcggtta
    26401 ccggagagaa aagggggtga ggttacatta cgtattcgta cctggtgggg catcgtcatt
    26461 tgtttttcac tggtgatttc aggtccacgc tggatgacgt tgacgttttt tgcgctgata
    26521 agttttctgg cattgaaaga atattgtacg cttatatcca tacattttcc gcggtggtta
    26581 tattgggtta ttcctcttaa ctatttgctt atcggtttta attgctttga gctgtttctg
    26641 ttatttatac ctttgtctgg gtttctgata ttagccacct ggcgagtttt tgtgggtgaa
    26701 ccctccggtt ttctgcatac cgtgagcgct attttttggg gctggataat gaccgttttc
    26761 gccttgagtc atgccgcctg gttattaatg ttgccatcca tgaatatcca gggcggggcg
    26821 ttactggtgt tatttctttt ggcattaacc gagtcaaacg atattgcaca gtatttatgg
    26881 gggaaatcct gcggcagaag aaaagtggtc cccaaagtca gcccgggaaa aacgctggaa
    26941 ggtctggtgg ggggcgtcat caccaccatg attgcgtcac tgattattgg accgttgctg
    27001 acaccgctaa atacatcaca ggcattatta gcgggtttgt taattggtat tagtggtttt
    27061 tgcggcgatg tagtgatgtc agccatcaaa cgagatattg gtgttaaaga tagtggaaaa
    27121 ctattaccag gacatggcgg actccttgac aggattgact cattaatttt caccgctccg
    27181 gtattttttt attttatacg ctactgctgt tactgaagga aaatagagaa aatggaaaat
    27241 tcacgcatct ctggggaaca tttttttacc accagtgata atacagcgtt gttttatcgg
    27301 cactggcccg ctttacagcc cggggcgaaa aaggtcatcg tcttatttca tcgtggccat
    27361 gaacattctg gtcgtctaca acatctcgtt gatgaactgg cgatgccaga tactgctttt
    27421 tatgcctggg atgcccgagg gcatggaaaa agttcggggc cgcgtggtta tagcccatct
    27481 cttgcgcgtt cagtgcggga tgtcgatgaa tttgtccgtt ttgctgccag cgacagccag
    27541 gttggactgg aagaggtggt tgtgatcgcg caaagcgtcg gcgcagtgct ggttgccaca
    27601 tgggttcatg attatgcgcc tgcaattcgc gggctggtgc tggcttctcc ggcctttaag
    27661 gttaaattgt atgtgccgct ggcacgtcct gcgctgacgc tatggcatcg tctgcgtggt
    27721 ctgtttttta ttaattccta tgtgaaagga cgctatttga cccacgatcg gcaacgggtg
    27781 gcgagtttca ataatgatcc gctgatcaca cgggcgattg ccgttaatat cttgctcgat
    27841 ctctacaaaa cgtctgaacg tattattaga gatgcggcgg cgattacgct ccccacgcaa
    27901 cttctgatat caggcgatga ctatgtggtg catcgccaac cgcagattga tttttatcag
    27961 agattacgta gccctctgaa agagctgcat ctgctgccag gcttttatca cgacacgttg
    28021 ggtgaagaga acagggcgca ggcatttgaa aaaatgcaaa gctttattag tcgtttatat
    28081 gctaacaagt cacaaaaatt tgattatcag catgaagacc gcactggacc atcagcggat
    28141 cgctggcggc tcctttcagg tggacccgtg ccattatcgc cggttgattt ggcgtatcgc
    28201 tttatgcgaa aggcgatgaa attgttcggg gcgcactctg cgggcctgca tctcggaatg
    28261 agcaccggct ttgattcagg cagttccctg gattatgtct atcaaaatca accgcaaggc
    28321 agtaacgcat tcgggcgctt aattgacaaa atctacctga acagtgttgg ctggcgcggt
    28381 attcgccagc gtaaaaccca tttacaaata ctgattaaac aagccgttgc cgatctccac
    28441 gccaaaggtt tagccgtccg cgtggttgac attgccgcag ggcatgggcg ctatgtactg
    28501 gatgcgctgg caaacgagcc tgccgtaagc gatattttgt tacgtgatta cagtgagtta
    28561 aatgttgcac aggggcaagc gatgattgcc caacggggaa tgtctgggcg ggtgcgtttt
    28621 gaacagggcg atgcgtttaa cccggaggaa ctcagcgcgt taactccgcg gcctacgctg
    28681 gcgattgtct ctggcctgta tgagcttttt cccgaaaatg agcaggtaaa aaactcactc
    28741 gcaggtcttg ccaatgccat cgaaccgggc ggcattctca tctacaccgg gcagccgtgg
    28801 caccctcaac tggagatgat tgccggggtg ttaaccagtc ataaagatgg taaaccgtgg
    28861 gtaatgcgcg tgcgttcgca aggggagatg gattcactcg tgcgtgatgc cggatttgat
    28921 aaatgcacac aacggattga tgagtggggt atttttacgg tttcgatggc ggtgcgtcgt
    28981 gataactgaa cgtcggaacg tattgctaca aggcgctggc tggttattat tgctggcccc
    29041 gtttttcttc ttcacctatg gatctcttaa tcagttcacc gcggttcagg accttaacag
    29101 ccatgatatc cccagtcagg tattcggttg ggaaacggcg atcccttttc ttccctggac
    29161 tattgttcct tactggagtc tggatctttt atatggattt tcgctgttcg tttgtagcac
    29221 gacattcgaa cagcgccgac ttgtccaccg gcttattctg gcaacggtaa tggcctgctg
    29281 cggttttttg ctctatccgc tgaagtttag ttttatccgt cctgaagtga gtggggtgac
    29341 gggatggcta ttttcgcaac ttgaactgtt tgatctgcct tataaccagt ctccttcgct
    29401 gcatattatt ctctgctggc tactttggcg tcactttcgt cagcatctgg ctgagaggtg
    29461 gcgtaaagtc tgcggcggat ggtttttact catcgctatt tctactctga cgacctggca
    29521 gcatcatttt attgatgtca tcacagggct ggcggtaggt atgttgattg actggatggt
    29581 gcccgtcgac cgtcgttgga attatcagaa acctgatcaa cgtcgaatca aaatagcact
    29641 gccctatgtc gtaggcgcgg gctcgtgcat tgtattgatg gagctaatga tgatgattca
    29701 gttatggtgg tcagtctggt tatgttggcc agtattatcg ctactcataa ttggccgtgg
    29761 gtacggtggg cttggcgcga taacaacagg gaaagatagt caggggaaac taccgcccgc
    29821 cgtttactgg ctgacattgc cctgccgcat cgggatgtgg ctgtctatgc gttggttttg
    29881 tcgtcgcctg gagccggtga gcaaaatgac tgctggtgtt tatttagggg cgtttccacg
    29941 acatattccg gcacagaatg cggttctgga cgtcaccttt gaattccctc gcggacgagc
    30001 gacaaaagat cgactctatt tctgtgtacc gatgctggat ctggtggttc cggaagaggg
    30061 ggagctccga caggccgtgg cgatgctgga aacattacgc gaagagcaag gcagcgttct
    30121 ggtccattgc gcgttgggat tatcgcgcag tgcgctggta gtggcggcat ggttgttatg
    30181 ttacggacac tgtaaaaccg ttgatgaagc gattagttat attcgagcca gacgttcgca
    30241 tattgtgctt aaggaagatc acaaagcgat gctgaaatta tgggaaaaca ggtaagtgga
    30301 ttgagatgtg gactgaacta cagtccacat caagatcgtg tccggttatg cagcaacaat
    30361 gctgtcgatg gccgctttcg cgtcagactg cgctttcgct gccatttccg gaccgtatgc
    30421 gatcccttcg gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga
    30481 cagatacggc gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat
    30541 aacgatggct tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt
    30601 aacgcctgcg cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt
    30661 atacatcggt gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc
    30721 ggaaagcgcc agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagtgc
    30781 accaaccagt tcgccatcca gtaccggaat cggatttgca gccaggtcgc gaacggtgat
    30841 ttcatcagcg gagtgctttt cgcgccattg ttcaacaaaa taatcggaca actgattaga
    30901 ctgagagtac cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc
    30961 ttatagatgt ttgaatgggc gatgccccgt tgcttgctga cactttattc acaatcctgc
    31021 cacagagata gcgcaataaa tcgaagccta tgttcgaatt tattgaacaa cgcatagaaa
    31081 gccgcgatgt ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc
    31141 ccactaacgt tatgacagaa caacaaaaat tgacctttac ggccttgcag cagcggctgg
    31201 attcgctgat gctgcgcgac agactgcgtt tttctcggcg tctgcacggt gttaagaagg
    31261 ttaaaaatcc tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg
    31321 cggcagggaa agtcctgctg cgtgaagcgg cacgaccgga aattacttat cctgacaatt
    31381 taccggttag tcagaaaaaa caggacattc tcgaagcgat tcgtgatcac caggtggtga
    31441 tcgtcgccgg ggaaacgggt tctggtaaaa cgactcagtt accgaaaatc tgtatggagc
    31501 tggggcgcgg gattaaaggg ctgatcggcc atacccagcc gcgtcgcctg gcggcaagaa
    31561 cggtggcgaa ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca
    31621 aagtgcgttt cagcgatcac gttagtgata acacgatggt caagctgatg accgacggta
    31681 tcctgctggc ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg
    31741 acgaagcaca cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc
    31801 tgccgcggcg tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct
    31861 tttcgcgcca ctttaataat gcgccgatta tcgaagtctc cggtcggacc tatccggtgg
    31921 aagtgcgcta tcgaccgatt gttgaagaag ccgacgacac cgagcgcgat cagttgcagg
    31981 cgatttttga cgccgtagac gaactgagtc aggaaagccc tggcgacatt ctgatcttta
    32041 tgagtggtga gcgggaaatt cgcgataccg ccgatgcgct gaacaagcta aatctgcgtc
    32101 ataccgaaat cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc
    32161 agtcgcacag cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg
    32221 taccggggat taaatacgtt atcgaccccg gtacagcacg tatcagccgc tacagctatc
    32281 gcaccaaagt gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta
    32341 aaggccgctg tgggcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt
    32401 tcctttcgcg cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta
    32461 ttttgcagat gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac
    32521 cggataaacg caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca
    32581 cggatgaaca ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc
    32641 ctgtcgatcc acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg
    32701 aggcgatgat tatcacgtcc gcgctttcca ttcaggatcc gcgcgagcgt ccgatggata
    32761 aacagcaggc atcggacgaa aaacatcgtc gcttccacga caaagagtct gacttcctcg
    32821 cgtttgtgaa tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct
    32881 tccgtcgcct gtgtcgtacc gattatctca actatctgcg cgtgcgcgaa tggcaggata
    32941 tctacaccca gttgcgccag gtggtgaaag aacttggcat tccggttaac agcgaaccgg
    33001 cggagtatcg cgaaattcac attgcgttgc tgacgggttt actttcccat atcggcatga
    33061 aagatgccga taaacaagaa tataccggcg cacgtaacgc gcgtttctcc atctttcccg
    33121 gttctggttt attcaaaaaa ccgccgaaat gggtaatggt ggcggaactg gtagaaacca
    33181 gccgcctgtg ggggcgcatt gctgcgcgta tcgacccgga atgggtggag cctgtcgctc
    33241 agcatttgat taaacgcacc tacagcgaac ctcactggga acgggcgcag ggcgcggtga
    33301 tggcaacgga aaaagtcact gtttatggtt tgccgattgt tgccgcgcgc aaggtcaact
    33361 acagccagat cgatccggcg ttatgccgtg aactctttat tcgccatgcg ctggtggaag
    33421 gtgactggca gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg
    33481 aagagctgga acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgttgtttg
    33541 agttctacga ccagcgcatc agccacgatg tgatctctgc acgccacttc gacagctggt
    33601 ggaaaaaagt cagccgcgaa acccctgatt tgctcaactt tgaaaaaagc atgttgatca
    33661 aagagggcgc agaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc
    33721 tcaagctgcg tttgagctat cagtttgagc ccggcgcgga tgctgacggt gtgaccgtac
    33781 atattccgct gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg
    33841 gtctgcgccg cgaactggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta
    33901 attttgtacc cgcgccaaac tatgccgaag cgtttttagg ccgcgtcaaa ccgctggagt
    33961 taccgttgct cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc
    34021 gcgaagactg gcactgggat caggtgcctg atcacctgaa aattaccttc cgcgtggtgg
    34081 atgacaaaaa caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga
    34141 aaggcaaagt gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct
    34201 tacatatctg gagttttggt cagctgccgg aaagctacga acagaagcgt ggcaactaca
    34261 aagtgaaggc gtggccggcg ctggtggatg agcgcgacag tgtggcgatc aaactgtttg
    34321 ataacccgct ggagcaaaag caggcaatgt ggaacggtct tcgccgtcta ctgctgctga
    34381 atattccatc gccaatcaaa tatttacatg aaaagttacc gaacaaagcc aagctgggac
    34441 tgtactttaa cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg
    34501 tggatcaatt gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc
    34561 tgcatgaaaa agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg
    34621 agcaaatcct cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga
    34681 ccatggcact ggggctttct gacattaaag cgcagatggg cgggttggta tatcgcggtt
    34741 ttgtcactgg taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg
    34801 aaaaacggct ggaaaaactg gcggttgatc cacatcgcga ccgtgctcag atgctgaaag
    34861 tcgaaaacgt ccagcaggcg tggcagcaat ggatcaacaa actgccgccc gcacgtcgtg
    34921 aggatgaaga cgtgaaagag atccgttgga tgatagaaga gttgcgcgtt agttacttcg
    34981 ctcaacaact tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc
    35041 agattagcgg ttaaccctgc tatttgcctg ataaagaaaa aacccggtaa gcatttagcg
    35101 ccgggttttt tattaattct aaaacggtaa gggtaaaaat tcaggaattc agaaaaatac
    35161 aattctctgc tgcaagatga ataatgttta tctacagcat ttccttaaaa gatatgtcag
    35221 gcttgcggag tggcggttaa ggacatacga cttcctcctt tcagagtgct ccgcttctca
    35281 ctattatctc acgcagtatt cttaagggaa cgataaggag gaaccatgaa cattaccccg
    35341 tttccgacgc tttcgccggc aactatagat gccataaatg ttatcggaca gtggctggcg
    35401 caggatgatt tctccggtga ggtgccgtat caggccgatt gcgtgatcct tgcaggcaat
    35461 gcggttatgc cgactatcga cgcggcatgt aagattgctc gcgatcagca aattccttta
    35521 ctgattagtg gtggtatcgg tcactcgaca acttttttgt atagcgccat cgcacagcat
    35581 ccgcactaca acactatccg caccactggc agagcagaag cgaccatcct ggcggatatc
    35641 gctcatcagt tctggcacat tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac
    35701 tgcggtgaaa acgcacgctt tagcatcgcg ctattgaatc aggccgtaga acgagttcat
    35761 acggctatcg ttgttcagga ccccaccatg cagcggcgca cgatggcgac gttccgccgt
    35821 atgactgggg acaatcccga tgcaccacgc tggttaagtt atcccggatt cgttcctcag
    35881 ttaggaaata acgcagacag tgtaatcttt attaatcagt tacaaggatt atggccggtt
    35941 gagcgttatc tctcactact cactggcgag ctgccgcgtt tacgcgatga tagcgatggc
    36001 tacggtcccc gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga agtcatccat
    36061 gcatggcaaa cgctgaaaca tgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta
    36121 cgttaaaaat tgcccgtttg tgaaccactt gtttgcaaac gggcatgact cctgactttt
    36181 atttctgcct tttattcctt ttacacttgt ttttatgaag cccttcacag aattgtcctt
    36241 tcacgatgcc gtctctctga tgattgatgt taattaacaa tacattcacc gaaaacaaac
    36301 atataaatca caggagtcgc ccatgtcagt acccgttcaa catcctatgt atatcgatgg
    36361 acagtttgtt acctggcgtg gagacgcatg gattgatgtg gtaaaccctg ctacagaggc
    36421 tgtcatttcc cgcatacccg atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc
    36481 agaacgtgca caaccagaat gggaagcgtt gcctgctatt gaacgcgcca gttggttgcg
    36541 caaaatctcc gccggcatcc gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga
    36601 agggggcaag atccagcagc tggctgaagt cgaagtggct tttactgccg actatatcga
    36661 ttacatggcg gagtgggcac ggcgttacga gggcgagatt attcaaagcg atcgtccagg
    36721 agaaaatatt cttttgttta aacgtgcgct tggtgtgact accggcattc tgccgtggaa
    36781 cttcccgttc ttcctcattg cccgcaaaat ggctcccgct cttttgaccg gtaataccat
    36841 cgtcattaaa cccagtgaat ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga
    36901 tgaaataggc cttccgcgcg gcgtgtttaa ccttgtactg gggcgtggtg aaaccgttgg
    36961 gcaagaactg gcgggtaacc caaaggtcgc aatggtcagt atgacaggca gcgtctctgc
    37021 aggtgagaag attatggcga ctgcggcgaa aaacatcacc aaagtgtgcc tggaattggg
    37081 tggtaaagca ccagctatcg taatggacga tgccgatctt gaactggcag tcaaagccat
    37141 cgttgattca cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt
    37201 acagaaaggc atttatgatc agttcgtcaa tcggctgggt gaagcgatgc aggcggttca
    37261 atttggtaac cccgctgaac gcaacgacat tgcgatgggg ccgttgatta acgccgcggc
    37321 gctggaaagg gtcgagcaaa aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt
    37381 gggtggcaaa gcagtagacg ggaaaggata ttattatccg ccgacattgc tgctggatgt
    37441 tcgccaggaa atgtcgatta tgcatgagga aacctttggc ccggttctgc cggtggtcgc
    37501 atttgacacg ctggaagatg ctatctcaat ggctaatgac agtgattacg gcctgacctc
    37561 atcaatctat acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg
    37621 tgaaacttac atcaaccgtg aaaacttcga agctatgcaa ggcttccacg ccggatggcg
    37681 taaatccggt attggcggcg cagatggtaa acatggcctg catgaatatc tgcagaccca
    37741 ggtggtttat ttacagtcgt aatgagtaaa agaggcggag gttttttcct ccgcctgtgc
    37801 gcgtcagagt ttagcgaatt tttcgagggt gcgaataagc tgcgtgacga agccatattc
    37861 gttatcgtac caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg taatttccgt
    37921 ttgcgtggca tcaaacaccg aaccgaaatg gctgccaatg atatcggaag agactatttc
    37981 ttcatcggta taaccaaatg actcgttatt ggtggttgct tgtttaagtg cgttattcac
    38041 ctcttcggca gtcacttttt ttccgagaat ggacaccagc tcagtgaccg aacctgtttt
    38101 caccggcacg cgttgcgcat gacctttcag tttgccgctc agttccggga tcaccagacc
    38161 aatggctttt gccgcccccg tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc
    38221 acgtaaatct ttaccacgtg ggccatccac cagtgactgg gtgccagtat aggcatgaat
    38281 ggtcgtcatc gtgccgactt ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc
    38341 aagacagtta gtggtgcatg acgccacgga aacaatggtg tcgttgccat ccagagtgtc
    38401 gtcattgacg ttataaacga tagttttcat ttcaccggca ggggcggaaa tcaacacctt
    38461 cttcgcacca gcatcaagat gcgcctgcga tttctcggcg gaggtataaa agccagtaca
    38521 ttcgacaatg atttctgcac ctttcgcttt ccacggaata tttttagcct ctttttcggc
    38581 gtaaaccgcg atacttttcc catcaacgat aagtgaatct tccgtaaaat caacgctcca
    38641 ggggaatggt ccgtagtttg aatcatgttt cagcaggtag gcgagaattt ttggggaagt
    38701 gagatcatta atagcgacaa cgtctatgtt gcttttgact tcaagtaatc gacgcaacac
    38761 cagtcgaccg atacgaccaa aaccgttaat accaacttta ctcatggttt tctcctgtca
    38821 ggaacgttcg gatgaaaatt gatcctttcc aggcttagcc caggattgtg ggatgggcaa
    38881 tctccattct tacagtgaaa cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat
    38941 tttgagaaaa gcccgttatg ttaacggaaa attatgttaa agcaggaaat gttatggaaa
    39001 ataaatactc aaggttacaa atcagcattc actggctggt ctttttgctg gttatcgcag
    39061 cgtattgcgc aatggagttt cgtggtttct tcccacgtag cgatcggcca ctcatcaaca
    39121 tggttcatgt ttcctgtggt atctcaattc tcgtgctgat ggtggttcgt cttttgttaa
    39181 gactgaaata cccaaccccg ccgattatac ctaagccaaa accgatgatg acgggactgg
    39241 cgcatttggg acatttggtg atttatctcc tgtttattgc gctgccagtg attggtttgg
    39301 tgatgatgta taaccggggc aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt
    39361 cagaggccaa tttcgaacgg gttgatagct taaagtcgtg gcatgagacg ctggcgaatc
    39421 tgggatattt tgtcatcggg ttgcacgctg cggctgcact ggcacaccac tatttctgga
    39481 aggacaacac acttctacgc atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa
    39541 acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat acgggtctac atcggaagcg
    39601 cctattatat ttatttgtat gacaaataaa accataatcc ttgcccatac gtccatctgg
    39661 cttattttta atcaattcac ccgatctttg atctcatcaa cggtatcaaa ataaaccagc
    39721 gtataacgtt caaatcgaac ataagattcg ataaaccatg gaggttatat gaaaaaactg
    39781 gcacttattt tgtttatggg aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc
    39841 tgttctggtt cgaaaggggg gatctcacac tgtacggcag gcggcaaatt tgtctgtaat
    39901 gatggttcta ttagtgcctc gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca
    39961 tgagaaaatg aatctgctag tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg
    40021 gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt cccggaaccg cagttcgtag
    40081 agcgtttgtc gggtcaggag tgtgaatgtc agaatcgtaa tgcagataat gagcagacac
    40141 accaccagag ggttgtgctt catagccttt ctccttgccg gatggcgggt aagaggctaa
    40201 gatctgaatt gctaggttca ttcgttggcc tcggttgata gaaatatcgg tcggggcctt
    40261 cgtctttctg attcccggtt agcctgaaaa cagaaagtct caggcacccg caggcatcct
    40321 atgaggtttc cttagggacg aaaataatca cttcacgaaa ttgcgtgctg ttttccagaa
    40381 tttttcgtca ttcgggttag ccagtttagc cattcgttac tctcttcatt ccaatagcat
    40441 taattttctc tgcaataatt gttgtaaaaa tgtgacgcaa agaggttttt ggtcataagt
    40501 aattaccgtc aagtgccgat gactttctat caggagtaaa cctggacgag agacaacggt
    40561 aatgaataca actccctcac atcgattagg ttttttgcat cacatcaggc tggttccgtt
    40621 atttgcctgc attcttggcg gtatcttagt tctattcgca ttaagttctg ctctggctgg
    40681 ctatttcctc tggcaggccg atcgcgatca gcgtgatgtt actgcggaga ttgagattcg
    40741 gaccgggtta gcgaacagtt cagatttttt gcgttcagcc cggatcaata tgattcaggc
    40801 cggggctgcg agtcgtattg cggaaatgga agcaatgaag cgcaatattg cgcaagccga
    40861 atcggagatt aaacagtcgc agcaaggtta tcgtgcttat cagaatcggt cggtgaaaac
    40921 acctgctgat gaagccctcg acactgaatt aaatcaacgc tttcaggctt atatcacggg
    40981 tatgcaacct atgttgaaat atgccaaaaa tggcatgttt gaagcgatta tcaatcatga
    41041 aagtgagcag atccgaccgc tggataatgc ttataccgat attttgaaca aagccgttaa
    41101 gatacgtagc accagagcca accaactggc ggaactggcc catcagcgca cccgcctggg
    41161 tgggatgttc atgattggcg cgtttgtgct tgccctggtc atgacgctga taacatttat
    41221 ggtgctacgt cggatcgtca ttcgtccact gcaacatgcc gcacaacgga ttgaaaaaat
    41281 cgccagtggc gatctgacga tgaatgatga accggcgggt cgtaatgaaa tcggtcgctt
    41341 aagtcgtcat ttacagcaaa tgcagcattc actggggatg acagtaggga ctgttcgaca
    41401 gggtgcggaa gagatttatc gtggcaccag cgaaatttca gctggcaatg cggacctgtc
    41461 atctcgcacc gaagaacaag cggcggctat cgaacaaact gccgctagca tggagcaact
    41521 cactgcgacg gtgaaacaga atgcggataa cgcgcatcat gccagcaaac tggcgcaaga
    41581 ggcttctatt aaagccagcg atggcgggca gacggtttcc ggtgtagtaa aaacgatggg
    41641 cgctatctct acaagttcga agaaaatttc cgagatcacc gccgtcatca acagtattgc
    41701 tttccagacg aatattctgg cactgaatgc tgccgttgaa gccgcgcgag cgggtgagca
    41761 aggccgtgga tttgccgttg tcgccagcga agtacggaca ctcgcaagcc gcagcgccca
    41821 agcggcgaaa gagattgaag gcttgatcag tgaatcagtc aggttaattg acctggggtc
    41881 ggatgaggtg gcaacggcag ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt
    41941 cacacatatc atgcaggaaa tcgccgccgc ctcggatgaa caaagtagag gcataacgca
    42001 ggttagccag gcgatttctg aaatggataa ggtgacgcaa cagaatgctt ctctggtaga
    42061 agaggcctca gcggcggcgg tgtcccttga agaacaggcg gcacgattaa ctgaggcggt
    42121 ggatgtattc cgtctgcaca aacattctgt gtcggcagaa cctcgcggag cgggtgaacc
    42181 agttagtttc gctacggtgt gaaaatgttc aaggagggat cgacagatcc cttcaccttt
    42241 cagaacggca ttgattttcg aatagcgtta atcatcaact ggcaaccaga agagaacgtc
    42301 gcatctacgc gggtcagtat tccaatcggt tcgcctgcac catgtcccgg aacaggcagg
    42361 gccaccagcg tggcatgacg caggtcgtct tttacagcgc cagaagggac aaaccacacg
    42421 taatcgtatt caaccgtaag ttgacgagat agcgaagcag acagcgtttc gatacaaccc
    42481 gaaggaattt tacagccctg gctctgcact aatgcatctg aatgctggcg tggcgcagtg
    42541 ccttctggtg atacaacgac cggccattcc agcacccggc ttagcgttac gttctcctga
    42601 agtagcgggt gattagggcg gacaaccagc ttcaacgatt caagaaacag cagttcgtaa
    42661 ttaagcccgg tcatcagttc aggatctgac atccgaccaa tgccgatatc gatttccccg
    42721 gtttttaaac ccgccagaat catagggtta ctcattgtcg caacttgcaa ggtcgtctct
    42781 ttttgttgct gatgaaactg acctataacc gaaggtaata tccccagtgc cgcagtaggt
    42841 agtgcaccaa ccctgacgac atcattatta agaccttctt tacgatgaag cgactgtccg
    42901 gcagtgttga tggcgtcaag aactctgact gcatgcgtta aaaattgttc gccgggtaag
    42961 gtaagttgcg ccccctgacg accacgctca aacaagcgag cgccagtcag ctgctccagt
    43021 tcattcaatg tcttagagag cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc
    43081 aaagttcctt gttgtgcgac agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga
    43141 ctaaacagac tatttttttc cataagcgat gttaaaaacg aagcggtgtc gctgacaagt
    43201 gaagttgttt gattatgata acttgattgc aaaat
//
LOCUS       DAPPNJ010000010        41714 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000010 DAPPNJ010000000
VERSION     DAPPNJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.010"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..714)
                     /locus_tag="RPM73_000404"
     CDS             complement(<1..714)
                     /locus_tag="RPM73_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDX6518799.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIK"
     gene            complement(785..2899)
                     /gene="fusA"
                     /locus_tag="RPM73_000405"
     CDS             complement(785..2899)
                     /gene="fusA"
                     /locus_tag="RPM73_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDX6518800.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2996..3466)
                     /gene="rpsG"
                     /locus_tag="RPM73_000406"
     CDS             complement(2996..3466)
                     /gene="rpsG"
                     /locus_tag="RPM73_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDX6518801.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3563..3937)
                     /gene="rpsL"
                     /locus_tag="RPM73_000407"
     CDS             complement(3563..3937)
                     /gene="rpsL"
                     /locus_tag="RPM73_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDX6518802.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(4063..4350)
                     /gene="tusB"
                     /locus_tag="RPM73_000408"
     CDS             complement(4063..4350)
                     /gene="tusB"
                     /locus_tag="RPM73_000408"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDX6518803.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4358..4717)
                     /gene="tusC"
                     /locus_tag="RPM73_000409"
     CDS             complement(4358..4717)
                     /gene="tusC"
                     /locus_tag="RPM73_000409"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDX6518804.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4717..5103)
                     /gene="tusD"
                     /locus_tag="RPM73_000410"
     CDS             complement(4717..5103)
                     /gene="tusD"
                     /locus_tag="RPM73_000410"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDX6518805.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5103..5825)
                     /locus_tag="RPM73_000411"
     CDS             complement(5103..5825)
                     /locus_tag="RPM73_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6518806.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5993..6805)
                     /gene="fkpA"
                     /locus_tag="RPM73_000412"
     CDS             complement(5993..6805)
                     /gene="fkpA"
                     /locus_tag="RPM73_000412"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX6518807.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            7026..7244
                     /locus_tag="RPM73_000413"
     CDS             7026..7244
                     /locus_tag="RPM73_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDX6518808.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7293..7883)
                     /gene="slyD"
                     /locus_tag="RPM73_000414"
     CDS             complement(7293..7883)
                     /gene="slyD"
                     /locus_tag="RPM73_000414"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDX6518809.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7978..8178)
                     /locus_tag="RPM73_000415"
     CDS             complement(7978..8178)
                     /locus_tag="RPM73_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDX6518810.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8188..9993)
                     /gene="kefB"
                     /locus_tag="RPM73_000416"
     CDS             complement(8188..9993)
                     /gene="kefB"
                     /locus_tag="RPM73_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDX6518811.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9993..10544)
                     /gene="kefG"
                     /locus_tag="RPM73_000417"
     CDS             complement(9993..10544)
                     /gene="kefG"
                     /locus_tag="RPM73_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDX6518812.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10675..12588
                     /locus_tag="RPM73_000418"
     CDS             10675..12588
                     /locus_tag="RPM73_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6518813.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12588..13610
                     /locus_tag="RPM73_000419"
     CDS             12588..13610
                     /locus_tag="RPM73_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX6518814.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13604..13822
                     /locus_tag="RPM73_000420"
     CDS             13604..13822
                     /locus_tag="RPM73_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDX6518815.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13876..14745
                     /locus_tag="RPM73_000421"
     CDS             13876..14745
                     /locus_tag="RPM73_000421"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDX6518816.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14800..15204)
                     /locus_tag="RPM73_000422"
     CDS             complement(14800..15204)
                     /locus_tag="RPM73_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDX6518817.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15506..16138
                     /gene="crp"
                     /locus_tag="RPM73_000423"
     CDS             15506..16138
                     /gene="crp"
                     /locus_tag="RPM73_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDX6518818.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16189..18279
                     /locus_tag="RPM73_000424"
     CDS             16189..18279
                     /locus_tag="RPM73_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX6518819.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18346..19566)
                     /gene="argD/dapC"
                     /locus_tag="RPM73_000425"
     CDS             complement(18346..19566)
                     /gene="argD/dapC"
                     /locus_tag="RPM73_000425"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDX6518820.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19652..20215)
                     /gene="pabA"
                     /locus_tag="RPM73_000426"
     CDS             complement(19652..20215)
                     /gene="pabA"
                     /locus_tag="RPM73_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDX6518821.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20247..20849)
                     /locus_tag="RPM73_000427"
     CDS             complement(20247..20849)
                     /locus_tag="RPM73_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDX6518822.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20839..21006)
                     /locus_tag="RPM73_000428"
     CDS             complement(20839..21006)
                     /locus_tag="RPM73_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDX6518823.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21111..21683)
                     /gene="ppiA"
                     /locus_tag="RPM73_000429"
                     /gene_synonym="rotA"
     CDS             complement(21111..21683)
                     /gene="ppiA"
                     /locus_tag="RPM73_000429"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDX6518824.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21954..23135
                     /gene="tsgA"
                     /locus_tag="RPM73_000430"
     CDS             21954..23135
                     /gene="tsgA"
                     /locus_tag="RPM73_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDX6518825.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23397..25940
                     /gene="nirB"
                     /locus_tag="RPM73_000431"
     CDS             23397..25940
                     /gene="nirB"
                     /locus_tag="RPM73_000431"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDX6518826.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25937..26263
                     /gene="nirD"
                     /locus_tag="RPM73_000432"
     CDS             25937..26263
                     /gene="nirD"
                     /locus_tag="RPM73_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDX6518827.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26389..27195
                     /gene="nirC"
                     /locus_tag="RPM73_000433"
     CDS             26389..27195
                     /gene="nirC"
                     /locus_tag="RPM73_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDX6518828.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27214..28587
                     /gene="cobA"
                     /locus_tag="RPM73_000434"
     CDS             27214..28587
                     /gene="cobA"
                     /locus_tag="RPM73_000434"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDX6518829.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28834..29001
                     /locus_tag="RPM73_000435"
     CDS             28834..29001
                     /locus_tag="RPM73_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDX6518830.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29245..30633
                     /gene="frlA"
                     /locus_tag="RPM73_000436"
     CDS             29245..30633
                     /gene="frlA"
                     /locus_tag="RPM73_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDX6518831.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTIGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAIMAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30654..31676
                     /gene="frlB"
                     /locus_tag="RPM73_000437"
     CDS             30654..31676
                     /gene="frlB"
                     /locus_tag="RPM73_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDX6518832.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31726..32556
                     /gene="frlC"
                     /locus_tag="RPM73_000438"
     CDS             31726..32556
                     /gene="frlC"
                     /locus_tag="RPM73_000438"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDX6518833.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32553..33338
                     /gene="frlD"
                     /locus_tag="RPM73_000439"
     CDS             32553..33338
                     /gene="frlD"
                     /locus_tag="RPM73_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDX6518834.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33438..34169
                     /gene="phoB"
                     /locus_tag="RPM73_000440"
     CDS             33438..34169
                     /gene="phoB"
                     /locus_tag="RPM73_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDX6518835.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34321..35406)
                     /locus_tag="RPM73_000441"
     CDS             complement(34321..35406)
                     /locus_tag="RPM73_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDX6518836.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35418..36722)
                     /locus_tag="RPM73_000442"
     CDS             complement(35418..36722)
                     /locus_tag="RPM73_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDX6518837.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36734..37087)
                     /locus_tag="RPM73_000443"
     CDS             complement(36734..37087)
                     /locus_tag="RPM73_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDX6518838.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(37098..37976)
                     /locus_tag="RPM73_000444"
     CDS             complement(37098..37976)
                     /locus_tag="RPM73_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDX6518839.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37973..39199)
                     /locus_tag="RPM73_000445"
     CDS             complement(37973..39199)
                     /locus_tag="RPM73_000445"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDX6518840.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLI
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39199..40362)
                     /locus_tag="RPM73_000446"
     CDS             complement(39199..40362)
                     /locus_tag="RPM73_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDX6518841.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40446..40808)
                     /locus_tag="RPM73_000447"
     CDS             complement(40446..40808)
                     /locus_tag="RPM73_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDX6518842.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40825..>41714)
                     /locus_tag="RPM73_000448"
     CDS             complement(40825..>41714)
                     /locus_tag="RPM73_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518843.1"
                     /translation="KKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAAL
                     KTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGLK
                     AQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALELGPHTY
                     VGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESLQRIVKG
                     DVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVDK
                     HALLAHQQRVVSGEQEPSY"
BASE COUNT        10017 a        11389 c        10505 g         9803 t
ORIGIN      
        1 tttgatgata ccgcgttcta cacgaccggt aacaacggta ccacgaccgg agatggagaa
       61 tacgtcttcg atcggcagca ggaacggctt gtcaatcgca cgctctggtt ccggaatata
      121 agaatccagg aagccagcca gttccaggat tttcgcttcc cactctgcgt cgccttccag
      181 cgctttcaga gcagaaccac gaacgatcgg agtgtcgtcg cccgggaagt cgtactgaga
      241 cagaagttca cgaacttcca tttcaaccag ttccagcagc tcttcgtcat caaccatgtc
      301 gcatttgttc aggaacacga tgatgtacgg aacgcctacc tgacgaccca gcaggatgtg
      361 ctcacgagtc tgcggcatcg ggccgtcagt cgcagcaact accaggatcg cgccgtccat
      421 ctgagcagca ccggtgatca tgtttttaac atagtcggcg tgccccgggc agtctacgtg
      481 tgcgtagtga cgggtcgggg tgtcatattc aacgtgagaa gtgttgatgg tgataccacg
      541 agctttttct tccggcgcgt tatcgatctg gtcgaatgca cgagcagcac cgccgtaggt
      601 tttagccagt acggtggtga ttgcagcggt cagagtagtt ttaccgtggt caacgtggcc
      661 gatagtacca acgttaacgt gcggttttgt acgttcaaat ttttctttag acacggctat
      721 attccttact atagtgctct ccccttcagg agagagcacg ggactttggt attaaccctt
      781 aggcttattt accacgggct tcaattacgg cctgagcaac gttactcggc gcttcatcat
      841 acttcaggaa ttccatagtg tatgatgcac gacctttggt cagagaacgc agctgagttg
      901 cgtatccgaa catttcagac agcggtactt cagcgtggat cttaacgcca gtaacttcag
      961 attcctgacc tttgagcata ccacgacgac ggctcaagtc accgataacg tcaccggtgt
     1021 tctcttccgg agtttctact tcaaccttca tgatcggctc aagcagaact ggtttcgctt
     1081 tcttaaagcc ttctttaaag gcgatagaag cagccagttt aaacgccagt tcagaggagt
     1141 caacgtcatg gtaagaaccg aagtgcagac gaatacccat gtctactacc gggtagcctg
     1201 ccagcggacc tgctttcagc tgttcctgga tacctttatc aacggccggg atgtattcgc
     1261 cagggattac accaccttta atgtcgttga tgaactcgta gcctttcggg tttgaacccg
     1321 gctccagcgg gtacatgtcg ataacaacat gaccatactg accacgacca ccagactgtt
     1381 tcgcgtgttt accttcaaca tcggtaactt tctggcggat agtttcacgg taagcaacct
     1441 gcggtttacc tacgttcgct tcaacgttga attcacgctt catacggtca acgatgatgt
     1501 cgaggtgcag ttcgcccata cccgcgataa tggtctggtt agattcttcg tcagtccata
     1561 cacggaaaga cgggtcttct ttagccagac ggcccagagc cagacccatt ttttcctggt
     1621 cagctttggt tttcggttca actgcgatgg agattaccgg ctcagggaat tccatacgtt
     1681 ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc agtggttacg tctttcagac
     1741 cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat ctcttcacgt ttgttagcgt
     1801 gcatctgaac gatacgaccg aaacgctcac gtgcagcttt cacggagttc agtacggtat
     1861 caccagagtt aaccacaccg gagtaaacac ggaagaaggt caggttacca acaaacgggt
     1921 cggtagcgat tttgaacgcc agtgcagaga acggctcgtc atcacttgcg tgacgctcag
     1981 ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc aggtacgtca accggggatg
     2041 gcaggtaatc aattaccgca tccagcatcg cctgaacacc tttgttcttg aacgcagaac
     2101 cacaggttac caggatgatt tcgttgttca gaacgcgctg acgcagagca cctttgattt
     2161 ctgcttcagt cagttcttca ccacccaggt atttttccat cagctcttca gaagcttcag
     2221 ctgcggattc gatcaggttc tggtgccatt cgttagccag ttcaaccatg tctgccggga
     2281 tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt ccagttgata gctttcattt
     2341 tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc aatcgccagc tgcagcggaa
     2401 ccgggttcgc gcccagacgg gttttgatct ggttaacaac tttcaggaag ttcgcaccca
     2461 tgcggtccat tttgttaacg aacgcaatgc gcggaacttt atatttgttt gcctgacgcc
     2521 atacggtttc agactgcggc tgaacaccac caactgcgca gtaaaccatt accgcaccat
     2581 cgagaacacg catggaacgt tctacttcga ttgtgaagtc aacgtgcccc ggggtgtcga
     2641 tgatgttgat gcgatgcggc tcatactgct tagccatacc agaccagaat gcagtagtcg
     2701 cagcggaagt gatggtaata ccacgttcct gctcctgctc catccagtcc atggttgcag
     2761 cgccgtcatg aacttcaccg attttatggt ttacaccggt gtagaacaga atacgttcgg
     2821 tagtagtggt tttaccggcg tcgatgtgcg cactgatacc gatattacgg tagcgtgcga
     2881 tgggtgttgt acgagccatt tgtttcctcg tttatctttt aggcgttcaa tttaagtagc
     2941 ccaaagcggg ctgcttactg gaagcgcccg cctggtgact aaaactccga agggattacc
     3001 aacggtagtg tgcgaacgcc ttgttggctt cggccatacg gtgaacgtct tcacgtttct
     3061 taactgcagt acctttgttt tctgcagcat cagaaagttc gttcgccagg cgcagagcca
     3121 tggatttatc accgcgttta cgagcagctt caacgatcca acgcattgcc agagcattac
     3181 gacgaaccgg acggacttca actggtacct gataagtaga accaccaacg cggcgagact
     3241 taacttctac agtcgggcgc acgttttcga gagctacttc gaatgcttcc agttcagatt
     3301 taccagagcg ctgagccagg gtctccagcg cgctgtatac gatagattca gcagtagatt
     3361 ttttaccatc taccatcagg atatttacaa atttagccag cagttctgat ccgaacttcg
     3421 gatccggcag aattttacgc tgaccaatga cgcgacgacg tggcatggaa atactccgtt
     3481 gttaattcag gattgtccaa aactctacga gtttagtttg acatttaagt taaaacgttt
     3541 ggccttactt aacggagaac cattaagcct taggacgctt cacgccatac ttggaacgag
     3601 cctgcttacg gtctttaacg ccggagcagt caagcgcacc acgtacggtg tggtaacgaa
     3661 cacccgggag gtctttaaca cgaccgccac ggatcaggat cacggagtgc tcctgcaggt
     3721 tgtgaccttc accaccgatg taggaagtca cttcgaaacc gttagtcaga cgaacacggc
     3781 atactttacg cagcgcggag ttcggttttt taggagtggt agtatataca cgagtacata
     3841 cgccacgttt ttgcgggcat gcttccagcg caggcacgtt gcttttcgca actttgcgag
     3901 cacgtggttt gcgtaccagc tggttaactg ttgccattaa atagctcctg gttttagctt
     3961 ttgcttcgta aacacgtaat aaaacgtcct cacacaatat gaggacgccg aattttaggg
     4021 cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc catcaccagg ccatctggct
     4081 ggagtgctta accgtaagtc tgacgaaatc agtatagtca atgggaatga tgtcgttcga
     4141 aatttgacca gtcaaaccgc gggcaataag gtcttcgttc agggcataga ccttaatggg
     4201 ggcattacgc agactttcaa ggtagcggtt accgtcaact gcggcagtta cgccatcttg
     4261 caataatagc agttcgtctc cttcactgag cagacgcagc agcgcagcaa aatccgtcag
     4321 ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca aaacctcaaa ataacatcgt
     4381 agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg cgtggcttcg acaacaaagg
     4441 gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc acaaacccag cactgttcaa
     4501 tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc acgcgccagc actgcatcgg
     4561 gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat aaagaagacg gtcagatcgt
     4621 cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc ccgaccagca gctgtaccat
     4681 gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag aactgtacca ccctgtcaca
     4741 ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag gtaaatcccg gctgaaggtt
     4801 tgacgaagcc agccccagcc ttcctgcttc agtttcatcg acgataccgc ggcgtaacgc
     4861 tgccgctacg cagatattca gcgccacacc atgttgcgca ttcaactgtt gccagccgcg
     4921 cacaaggtca aattcgtcac ttgccggtga ggtcaattgg ttagcgttat agaccccttc
     4981 ccgatagaaa aagacgctgc ttaactcatg gccttctact atcagcgcct gcgcaaactg
     5041 aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg gtcaccacga tggcaaaacg
     5101 cattacttat cttgcccctg gaaatcaccg cttttgaact ggcggatgta gagatagaca
     5161 gtgtgtttgg agatgttcag gcggtcagca acctggttga tcgcgtcttt aatatcgaag
     5221 atccctttct cgtagagatt cagcacgatc tgacgatttt tggcgttatt agaaacattg
     5281 cgatcggcat tcacttcttc gatggtgaac tccagcgttt gggtaaccag atcttcaaca
     5341 gaagaggcaa agttgacgct tgaaccgaca tccggggttt ctggcggcac aaaagtgctc
     5401 ataatctgcg agaagggaac atcaagattc atattgatgc acagcagacc aattacacgc
     5461 tgttcgcggt tacgaatcgc gatagtcagg gacttcatta atacgccgct tttggcgcga
     5521 gtaaagtagc atttagaaac gctgctatcc gctcccgtca tatcgtgcag catacgtagc
     5581 gccaggtcag taattggcga accaatcttc cggcctgtat gttcaccgtt agcaatgcga
     5641 atggctgaac attttagatc ctgcaaagag tgcaaaacga tttcacagtg ggagccaata
     5701 agcatcgcta acccgtccac caccgcttcg taggatttca gaatatcaaa atcggtctgg
     5761 tcgaaaggac gttgatccag taaatccaac tcactggttt cgttggttaa aagcgacctg
     5821 gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta acttttcagg gcaggctcat
     5881 taatgatgcg ggtaactaaa ttaatacagc ggaggttccg ctttccagca ctaattatat
     5941 ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg gttcttacat gcttattttt
     6001 tagcagaatc tgcggctttc gcatcagctt ccggctttgc atcagccttc ggcgctggtt
     6061 tcacatccag cagctctacg tcaaacacca gggtagaatt cggtgggatc cccggaacac
     6121 ccgctttgcc gtaagccagt tctggtggaa taaccagttt gatcttaccg cctttcttga
     6181 tgttcttcag accttctgtc caacccggga taacaccgtc cagacggaaa gaaagcggtt
     6241 caccacgggt gtaagagttg tcgaactctt taccgtcgat cagcgtacct ttgtagttca
     6301 ctacaacagt atcgctgtct ttcggtgctt cgcctttacc ggcttctact acctgataaa
     6361 ccagaccagt tgaagaggtt ttcacacctt tctctttggc aaatttctcg cggtactctt
     6421 taccttttgc ttcgttatca gccgcgtctt tttccatctt cgcctgagca gaagacttca
     6481 cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg gtcggagagt ttgctcttat
     6541 cagcaaatgc atcctgaaca ccagcgatca gctgatcttt atccagtttg atgcccagtt
     6601 tttcttgttc tttaagagag ttttccatgt aacgacccag cgaagcaccc agtgcataag
     6661 ctgatttctg atcgtcattt ttgaacgctg ctttgctgtc agcagttgta gcaggttttg
     6721 cagcttcagc agcaaaagtg attggtgcat gcagggcaac ggccattgtg gtcgccagca
     6781 gcgttacttt aaacagtgat ttcatccata tctccaggat cggggcatct caccccaggg
     6841 ttaactatta tcagaagggt actataaagc gttgtcgaac aaatctacat acagacacgc
     6901 cctattatca tctattttca gactcttttt gtttaaatta gtttcgatga ccgcgaaatg
     6961 agtgctgtct cgggcagcaa agttaagtag aatccgcggc gaccattcga caaaagaggt
     7021 gaatcatgca ggatttatca ttggaagcac gcctggcaga gctggagagc cgactggctt
     7081 ttcaggaaat caccattgaa gaactgaacg tcacggtgac cgctcatgaa atggagatgg
     7141 cgaaactgcg cgatcatctg cgtctgctga ccgagaagtt aaaagccagc cagccgtcga
     7201 acatcgcgtc gcaggctgaa gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg
     7261 ggattccccg cttttttgtc accttttcgg tattagtggc aaccacaacc gccgttgcct
     7321 ttaccgccac agcagccttc gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg
     7381 cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg cgccgtgaac gtggccatga
     7441 gccagttctt cttcggtcgc ttcgcgaatc gccacaactt caacgttgaa tttcaggttc
     7501 tgaccggcca gcatgtggtt accatcaacc acgacgtgat cgtcttcaac cgcagtgatt
     7561 tcaaccggta ccggaccctg gtcagtttca gccaggaaac gcatacctac ctgcagttca
     7621 tcaacgccca taaatacgtc tttaggaaca cgttgcacca ggttttcgtc gtactgaccg
     7681 taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc caacttcatg accttccagc
     7741 gccgtttcca ggccagagat cagggaaccg tgaccatgca ggtagtccag cggcgcactc
     7801 accggagact catcaaccaa cacaccgtct tctgtacgta cctgataggc caggctgacc
     7861 accaggtctt ttgctacttt catgatatct cctgagcatg ggaagaatag tggcgcagat
     7921 tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa aaactcggcg catatcgcta
     7981 gtccggatga aaaatcccga tcacttgctc atctttgcga acgtgatcgc gggcttcttt
     8041 gtctgcttct cgcatctgat gtccgcactt aacacattca acaatatcaa tattattttc
     8101 gcgccacatc gccattgaat cctgcgcctg acaggccggg cattttgcgc ccgcaataaa
     8161 acgttttcgg attgccatct ttaccctcta ctcaaattca tcccagccgt ccagctggcg
     8221 tcgttcttgt tgcatttcac gttggaaaat ctcttccagt tcgcgtcggg cttccctggc
     8281 gcgagaaatt tgtacggtat cagcatgcat cgggattaac tcccgcagca ttcgcatatc
     8341 caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc ggatgcatgc caagcgtgac
     8401 cagcgtcttg cgccccagct ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac
     8461 ccctgcctgt aataactcat gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa
     8521 atgcggaaag tgctgctggc atatttccac cagcttcatg gtgtcttccg gctcgttaca
     8581 ggtaatgacg atagactcag cggcctctgc acccgcagaa cgtaaaagat cgacctgcgt
     8641 ggcgtcgccg taataaactt tgtagccata tttacgcatc aggttaacgg cgctgatatc
     8701 ccgctccagc acggtaatgc gcattttatt tgccatcagc aaacgaccaa tcacctgacc
     8761 aaaacgcccg aagcccacga caatgacctg gggtttatca tcgttgaccc acggtttttc
     8821 gtcttcttct tccggtccgt taaactggcg ggatagccat ttatccacca gcttcatcag
     8881 caacggcgtg gtcatcatgg aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc
     8941 ctggaataag cgttgtgaag aagcggtaga aaagaggaca aaggcaaact caccgccctg
     9001 actcaacacg ccagcaaact gcatccgctc tgagctacgc acgccataca atcgcgccag
     9061 cagatacaac acgagaattt tcaccgccac cagcacaacc acgcttatca ctacccacaa
     9121 cagatgggta taaagcaccc cgagattgag cgacatgccg acagagataa agaacaaacc
     9181 gagcagcaag cctttgaagg gatcgatagc cgtttccagt tcatggcgat attcactttc
     9241 cgccagcagc acgcccgcaa taaacgtacc gagcgccatc gacagcccca gcgcgtccat
     9301 aaacaatgcg gaacccaaca ccaacagcag cgtcgcggcg gtgaacactt cccgcacgcc
     9361 agaagctgca ataaaacgga ataccggacg cagtaaatag cgcccgccaa tcagcatgcc
     9421 gacaaacgcc agcaccttca tgccgatctt catccagtcg aaatgttcgt ctgccgaacc
     9481 cgccaacaac ggcactaacg ccagtgccgg gatcaccgcc agatcctgaa acagcagaac
     9541 cgaaaacccg agctgcccgg attcgctgcg attcatccct ttctcacgca tcaattgcaa
     9601 cgccattgca gttgaagaca tcgcaaggcc aatgccaccg acaaccgccg cctgccaggc
     9661 gaaatccgtc agcatcaata atcccgccag caacgccgcg cttaacagca cctgtgccgc
     9721 gcctacgcca aaaatcgaac gccgcagttg ccaaagtttg gagggattca actcaagacc
     9781 gatgataaac atcaggaata ccacgccgag ttccgaaaag tggaggatct catcgacgtc
     9841 gctaataaac cccagccccc atgggccaat tgcaatccct gccagcaaat atcccaacac
     9901 agcgccaata cccagccgcg atgccagcgg caccgcagcc accgccgcga agagaaacag
     9961 cactcctgcg agtaaaaaat cggaaccttc catcagcggc ctcctggaga cagcggattt
    10021 gccagccagt caccgtaggc tctggcgtga ctcgccagct cctgtgcgct ttgccgtctc
    10081 gcccagtaaa taatgatggg acttaaccaa tgcatccggc acatgcccgc cgccagttca
    10141 aagggacgca gaacatcgct catcgggtag cgattcaatg cgtcataacg gtaagcactt
    10201 tccggctcgc cggtggtaat cacgctacgc cagtactttc ccgccagttg gtttcctccc
    10261 ggcccgctgg caaaaccacg acttaatacc cggtccagcc actctttcag tagcgccggg
    10321 cagctatagg tataaagagg atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc
    10381 tgctcacggg ggatatcaat aaaaaaatcg ggatagtgcg cataaaggtc gtgcacggta
    10441 acattgctga gctgcgtggc cggtttaagc agtacccggt ttgccaccga gtcctgagat
    10501 tccggatggg catacagcag caaaactttc gctggctgag acatcatccc cctcccggta
    10561 tggtttttgt gtatagtcgc tgttttgggc taccattgcg cccggtgcgg cagctcgccc
    10621 atacattaca ttatcataat gataagttaa catagtctga acatacggca ccttatgatt
    10681 gttttctcct cgttacaaat tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc
    10741 accatcaacc ctgggcagaa agtcggcctg gtgggtaaaa acggctgtgg taaatctacc
    10801 ctgctggcat tgctgaaaaa tgaaatcagc gccgacggcg gcagctacac ctttccggga
    10861 agctggcaac tggcgtgggt gaatcaggaa acgccggcgt taccgcaagc ggcgctggaa
    10921 tatgtcattg acggcgaccg tgaatatcgt caactagaag cgcagctaca cgacgccaac
    10981 gaacgtaacg acgggcacgc cattgcgacc attcatggca agctggatgc tattgacgca
    11041 tggagtattc gctcccgtgc cgccagcctg ctgcacggcc tcggtttcag caatgaacaa
    11101 ctggagcgcc cggtaagtga tttctccggt ggctggcgta tgcgtcttaa ccttgcccag
    11161 gcgctgattt gccgttcaga cttgctgctg ctcgacgaac cgactaacca cctcgatctc
    11221 gatgccgtta tctggctgga aaaatggttg aagagctatc agggcacgct gatcctgatc
    11281 tctcacgacc gcgacttcct cgatccgatc gttgataaaa ttattcatat cgaacaacaa
    11341 agcatgttcg agtacaccgg caactacagt tcgtttgaag tacagcgcgc cacccgtctg
    11401 gcgcagcaac aagcgatgta tgaaagccag caggaacgcg tagcgcatct gcaaagttat
    11461 atcgaccgtt tccgtgccaa agccaccaaa gcgaagcagg cccagagccg cattaagatg
    11521 ctcgagcgta tggagctgat tgcccccgcg cacgtcgaca acccgttccg ctttagcttc
    11581 cgcgcgccgg aaagcctgcc aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat
    11641 ggcgatcgca ttattctcga ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt
    11701 ctgttaggcc gcaacggcgc gggtaaatcg acattaatca aactgttagc cggtgaactt
    11761 gcgccagtca gcggtgaaat tggtctggcg aaagggatca agctcggcta cttcgcccag
    11821 catcaacttg aatacctgcg cgccgacgaa tcacctattc aacatctggc acgtttagcg
    11881 ccgcaggagc tggaacaaaa actgcgtgac tacctcggcg gctttggttt ccagggcgat
    11941 aaagtaaccg aagaaacgcg ccgcttctca ggtggggaaa aagcccgcct ggtgctggca
    12001 ttaattgtct ggcagcgtcc gaatctgctg ctgctcgacg aaccgactaa ccaccttgac
    12061 ctcgacatgc gtcaggcact caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc
    12121 gtttcgcacg accgtcattt gctgcgttcc accactgacg atctctacct ggttcacgat
    12181 cgtaaagtcg aaccgttcga cggcgatctg gaagattatc aacagtggtt gagcgacgta
    12241 caaaagcagg aaaaccagac cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca
    12301 cgtaaagatc agaagcgtcg ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa
    12361 gagattgccc gtctggaaaa agagatggag aagctgaacg cgcaactggc gcaggcggaa
    12421 gagaaactcg gcgacagcga actgtatgac cagagccgta aagcggagtt gaccgcctgc
    12481 ctgcaacagc aagccagcgc caaatccggc ctggaagagt gcgaaatggc gtggctggaa
    12541 gcccaggagc agcttgagca gatgctgctg gaaggccaaa gcaactgatg gcgcagataa
    12601 cgacgaccga tgccaatgaa tttagcagca gtgctgaatt cacccctatg cgcggcttta
    12661 gcaattgtca tctacaaact atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc
    12721 cgtactggca gcggctggag ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag
    12781 accccgcaca ggcgaaacat aaaccgcgtt tagtggtgtt tcacgggctg gaaggcagtc
    12841 tcaatagccc ttacgcccac ggtctggttg aggcggcgca aaagcgcggc tggctgggcg
    12901 tggtgatgca ttttcgcgga tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt
    12961 cgggcgaaac cgaagacgcc agttggtttt tacgctggct gcaacgcgaa tttggtcatg
    13021 cgccaacggc tgccgtcggc tattcgctcg gcggtaatat gctggcctgt ttgctggcaa
    13081 aggaaggcaa tgatctcccg gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg
    13141 aagcctgtag ctatcatatg gaaaagggct tttcccgcgt ttatcagcgt tacttgctaa
    13201 acctgttaaa agccaatgcc gcacgcaagc tggcagccta ccccggaaca ctgccgatta
    13261 atctcgcgca gttaaaatcg gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca
    13321 gaattcacgg ctacgctgac gctatcgact attatcgtca gtgtagcgcc atgccgatgc
    13381 tgaaccggat cgccaaacct acgctgatta ttcacgccaa agacgatccg tttatggatc
    13441 atcaggtgat cccgaaaccg gaaagtctcc ccccgcaggt tgagtatcaa ctgactgaac
    13501 atggcggtca tgttggcttt attggcggta cattacttca tccgcaaatg tggctggagt
    13561 cacgcattcc tgactggtta acaacgtatc tggaggcgaa atcatgttga ttccgtggca
    13621 agacctctcc cccgaaacgc tggaaaattt aattgaaagc tttgtgttac gtgaaggcac
    13681 cgattatggt gaacatgagc gtacacttga acagaaagtc gccgacgtca aacgccagct
    13741 acagtgcgga gaagcggtgc tggtatggtc ggagctgcac gaaacggtca atattatgcc
    13801 gcgcagtcag tttcgcgaat aacctgcaac cgtcggcgga cgagataata atgatagcta
    13861 agtcatggag ttactatgtc tgccaaacat ccggtcattg cggtaacagg atccagcggc
    13921 gcggggacca ccaccaccag cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat
    13981 gcagctgagg tggaaggcga cagttttcac cgttacaccc gcccggaaat ggacatggcg
    14041 atccgcaaag cgcgcgacgc cgggcggcat atcagctact tcggccccga ggctaacgac
    14101 ttcggcctgc tggaacaaac cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc
    14161 aaatatctgc atacctacga cgaagccgta ccgtggaatc aggtaccggg gacattcacc
    14221 ccctggcaac ctttaccgga acccactgat gtactgtttt atgaaggttt acacggcggc
    14281 gtagtcacgc cacagcataa cgttgcgcag catgtggact tactggtcgg cgtggtgcct
    14341 atcgttaacc ttgagtggat tcaaaaactg atccgcgaca ccagcgagcg cgggcactca
    14401 cgagaagcag tgatggactc ggtagtgcgt tcaatggaag attatatcaa ctacatcaca
    14461 ccgcagtttt cccgcaccca tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac
    14521 ccgttcgcgg caaaaggtat cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt
    14581 aatctggaag ggatcgattt cccctggctg ctggcgatgt tgcaaggctc attcatttcc
    14641 cacatcaata cgttagtggt accgggcggc aaaatgggtc tggcaatgga attaattatg
    14701 ctgccgctgg tgcaacgatt gatggaagga aagaaaatcg agtaactctg ctattacgcc
    14761 ggataaaata ctatccggct tcacaacggg atagttaaat cacgcggcaa ccacttcata
    14821 cgagtgagta atattcaccg ctttttccag catcaacgcc actgaacaat atttctcagc
    14881 agagagatca accgcacgcg caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa
    14941 atgcagatta atgtgcgtaa acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt
    15001 tacttcacaa tcgaccacat cctgacgccc tttttgcagg atagaaacca catcgatggc
    15061 actgcaaccc cccgccgcca tcaacaccat ttccatcgga cttggtgctt tatcgcctga
    15121 gttgccgtcc attaaaatct gatgaccaga ggcggattcg cccagaaaag ttaacccttc
    15181 gacccacttc actcgcgctt gcatttttgc tactccactg cgtcaatttt cctgacagag
    15241 tacgcgtact aaccaaatcg cgcaacggaa ggcgacctgg gtcatgctga agcgagacac
    15301 caggagacac aaagcgaaag ctatgctaaa acagtcagga tgctacagta atacattgat
    15361 gtactgcatg tatgcaaagg acgtcacatt accgtgcagt acagttgata gccccttccc
    15421 aggtagcggg aagcatattt cggcaatcca gagacagcgg cgttatctgg ctctggagaa
    15481 agcttataac agaggataac cgcgcatggt gcttggcaaa ccgcaaacag acccgactct
    15541 cgaatggttc ttgtctcatt gccacattca taagtaccca tccaagagca cgcttattca
    15601 ccagggtgaa aaagcggaaa cgctgtacta catcgttaaa ggctctgtgg cagtgctgat
    15661 caaagacgaa gagggtaaag aaatgatcct ctcctatctg aatcagggtg attttattgg
    15721 cgaactgggc ctgtttgaag agggccagga acgtagcgca tgggtacgtg cgaaaaccgc
    15781 ctgtgaagtg gctgaaattt cgtacaaaaa atttcgccaa ttgattcagg taaacccgga
    15841 cattctgatg cgtctgtctg cacagatggc gcgtcgtctg caagtcactt cagagaaagt
    15901 gggcaacctg gcgttcctcg acgtgacggg ccgcattgca cagactctgc tgaacctggc
    15961 aaaacaacca gatgctatga ctcacccgga cggtatgcaa atcaaaatta cccgtcagga
    16021 aatcggtcag attgtcggct gttctcgtga aaccgtggga cgcattctga agatgctgga
    16081 agatcagaac ctgatctccg cacacggtaa aaccatcgtc gtttacggca ctcgttaatc
    16141 ccgtcggagt ggcgcgttac ctggtagcgc gccattttgt ttcccccgat gtggcgcaga
    16201 ctgatttatc accccgatat caactatgca cttcgacaaa cgctggtgct atgtttgccc
    16261 gtggccgttg ggttaatgct tggcgaatta cgattcggtc tgctcttctc tctcgttcct
    16321 gcctgttgca atattgcggg ccttgatacg cctcataaac gttttttcaa acgcttaatc
    16381 attggtgcgt cgctgtttgc cacctgtagc ttactgacac agctgctact ggcaaaagat
    16441 gttcccctgc cctttttgct gaccggatta acgctggtac ttggcgtcac tgctgagttg
    16501 gggccattgc acgcaaaatt gcttcccgca tcgctgctgg cagccatttt taccctcagt
    16561 ctggcgggat acatgccggt ctgggaaccg ttgctcatct atgcgttggg cactctctgg
    16621 tacggattgt ttaactggtt ttggttctgg atctggcgcg aacaaccgct gcgcgagtca
    16681 ctaagtctgc tgtaccgtga actggcagat tattgtgaag ccaaatacag cctgcttacc
    16741 cagcacaccg atcctgaaaa agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc
    16801 gatctaatta cccagtgcta tcagcaaatg catatgcttt ccgcgcaaaa taatactgac
    16861 tacaagcgga tgctgcgtat tttccaggag gcgctggatt tacaggaaca tatttcggtc
    16921 agtttgcatc agccggaaga ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt
    16981 atccgctgga atgcgcaaac cgtcgccgct cgcctgcgcg tgctggctga tgacattctt
    17041 taccatcgcc tgccaacgcg ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc
    17101 gcccgccagc atccggataa tccggttggg caattctgct actggcattt cagccgcatc
    17161 gcccgcgtgc tgcgcaccca aaaaccgctc tatgcccgtg acttactggc cgataaacag
    17221 cggcgaatgc cattacttcc ggcgctgaaa agttatctgt cactaaagtc tccggcgcta
    17281 cgcaatgccg gacgactcag tgtgatgtta agcgttgcca gcctgatggg caccgcgctg
    17341 catctgccga agtcgtactg gatcctgatg acggtattgc tggtgacaca aaatggctat
    17401 ggcgcaaccc gtctgaggat tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt
    17461 gcgggcgtgg cgctgcactt taaaattccc gaaggttaca ctctgacgtt gatgctgatt
    17521 accaccctcg ccagctacct gatattgcgc aaaaactacg gctgggcgac ggtcggtttt
    17581 actattaccg cagtgtatac cctgcaacta ttgtggttga acggcgagca atacatcctt
    17641 ccgcgtctta tcgataccat tattggttgt ttaattgctt tcggcggtac tgtctggctg
    17701 tggccgcagt ggcagagcgg gttattgcgt aaaaacgccc atgacgcttt agaagcctat
    17761 caggaagcga ttcgcttgat tcttagcgag gatccgcaac ctacgccact ggcctggcag
    17821 cgaatgcggg taaatcaggc acataacact ctgtataact cattgaatca ggcgatgcag
    17881 gaaccggcgt ttaacagcca ttatctggca gatatgaaac tgtgggtaac gcacagccag
    17941 tttattgttg agcatattaa tgccatgacc acgctggcac gggaacaccg ggcattgcca
    18001 cctgaactgg cacaagagta tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag
    18061 cgactggagt atgacgaacc gggtagttct ggcgatgcca atatcatgga tgcgccggag
    18121 atgcagccgc acgaaggcgc ggcaggtacg ctggagcagc atttacagcg ggttattggt
    18181 catctgaaca ccatgcacac catttcgtcg atggcatggc gtcagcgacc gcatcacggg
    18241 atttggctga gtcgcaagtt gcgggattcg aaggcgtaat gcaggctaaa ccatcggata
    18301 aggcattcac gccgcatccg acatcttttg cctgatgctt cgtatttacg ccccaaccac
    18361 cttcgccacc gcgtgggcga aacgttgcat cccttcatcg atatccgcat cttccaccac
    18421 cagcgacggt gcaaaacgca tcacatccgg tccggcattc agcaccatta cgccagcctc
    18481 tgcgcccgca tacaggaaat cacgcgcccg acctttgtac tgtggtttca gctctgcgcc
    18541 aatcaacagc cccataccgc gaatatcgct aaacacatcg tactgctgat cgatcttctg
    18601 cagatggtca acaaaacgct ggcgtttcgc ctgaatgcct tccagcactt caggggtatt
    18661 gatgatatca aacgccgccc ccgctactgc acaggccaga ggattaccgc cgtaggtgga
    18721 accgtgagaa ccaggatgaa acgcagaagc aatttctgcc gtggtcagca tggcgctaat
    18781 cgggaagccg ccgcctaacg ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg
    18841 catgtaagca aacaaatcgc cggtccgccc catcccgcac tgtacttcat caaacaccaa
    18901 taatgcctga tgttgatcgc acagctcgcg caagccctgc aaaaactctg gcgtcgctgc
    18961 cgtcacaccg ccctcgccct ggatcggctc aaccaccacc gcacaggtgt gatcatccat
    19021 caccgctttc actgcgtgga gatcgttaaa aggaacgtgg atgatgtctg ccggttttgg
    19081 cccaaagccg tcggaatatt ttggctgccc gcccaccgag acggtaaaca gcgagcgacc
    19141 atgaaaagcg ttatggaagg caataatttt ggttttgaac ggactatgac gcacacaggc
    19201 gtaatggcgt gccagtttaa aggcggtttc gttagcttcc gtgccggagt tcataaacac
    19261 cacgcgttcg gcaaacgtcg cctcaatcag tttgcgccca agacgcagcg ccggttcatt
    19321 ggtgaaaacg ttactgatat gccacagagt ttcgccctgg gtttttaacg cgttcaccag
    19381 cgcaggatgg caatggccca acgccgtaac tgcaatgcca cccgcgaaat cgacatactc
    19441 cttgccttgc tgatcccaga ttcggctgcc ctgacctttt accggaatga actctgccgg
    19501 agcataaatc ggcaggatca cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat
    19561 tgccatctca tgatcaccct gttacgcata aataaatgtg aaattataat cacaaaatat
    19621 gcataaaaaa tcattgaatg gcaatcagaa atcagcgatg caggaaatta gccagcagtt
    19681 gatgtccttg ttcgctaaga atactttctg gatggaactg cacaccttcc agatcccact
    19741 ggcgatggcg aatccccata atctcgcggg tttcgctcca ggccgtcacg tcaaagcatg
    19801 ctggtaatga gccaggttcc accaccagcg aatggtagcg tgtcacggta agtggatttg
    19861 ccagcccccg aaatacgccc tcaccgttat gtgtaatcgg cgaggttttg ccgtgcatga
    19921 cctttgcggc gcgcacaact ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc
    19981 agacgccaag aatcggcaag cgcccggcat agtggcgaat aacgtcaagg gagatcccgg
    20041 cttcatctgg cgtacagggg ccgggtgata tgacaatttt ttgtggttta agggcgtcga
    20101 tatccgccag cgtcaacgca tcgttgcgct taaccagcac atccgccccc agttcacaaa
    20161 agtactggta gaggttccag gtaaaagaat cgtagttatc tataagcagg atcatggcgg
    20221 ctccggtacg aaagaaccgc gctattttac tcagattccc cggcttcgct taccactttg
    20281 ctaaatatca tctgcagcgc ggtgagatcc cccattgccc cactctgatt tgcctgattc
    20341 caggcctctt tttcgatacc ctgccagctc agttgatacc cggcatgaat cgccagttgc
    20401 tcgaagaaga tccgctgtgc cagaccactt cccacccgga agggatgcag cacgttgatt
    20461 tcacaatagt aatgcgccag ccgctcgacg aacttcgctt tctccaggcc aaccagataa
    20521 ccttcttcct ccagatcctg catcagggca ttgccctctt tttcgatata agcaaagtgg
    20581 cagaacggcg tatcgccctg ataaatatca acttcacgca gttgccccgc ccagtcgaaa
    20641 atatcctgat acagctggcg atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga
    20701 ccaagctcaa tggtcgcagc acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc
    20761 tgctgctggt ggatgttcag ccggttacgc atgatatcaa ggccaggata aagatacgga
    20821 tcgcgccctt cgccgaattt atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa
    20881 agcctctgcg gcagtaagag tgactaaagg catctcgacg ccttcaagac ggcgactggc
    20941 ctggaaatta cgattacgct gaagctccca gagacgggac ttttgcttat cggtgagttt
    21001 cttcacttga tgcctccctg aacgtgtcca tttgccacaa gtataagcgg caaatccggg
    21061 ttacgccggg agagcagaag cgcgggcaag attgcccgcg cgaaaaatca ttacggcagg
    21121 actttagcgg aaaggataac taccggtttt gacggcacat tctggtacgg accaacgtca
    21181 tgagtcggca cctgggaaat cttatcggca acgtccatgc ctttcaccac tttaccaaat
    21241 accgcgtaac cgaaatcacg ctgaccatgg tcaaggaagg cgttatcggc aacgttgata
    21301 aagaactggc tggtggcgct gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc
    21361 gtgttgcgca ggccgttatc ggcttcattt ttgattggcg ggtttggttt tttctgctgc
    21421 atctgctcgg tgaaaccgcc gccctgaatc ataaagccag gaatgacgcg gtgaaaggta
    21481 gtgttgttat aaaaaccgct gttcacataa tcgacaaagt tttgcacaga cactggcgct
    21541 ttttgtttat ccagctccag ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg
    21601 tcccctttcg ctgccattgc tgcgggagaa agagcagaaa gagcgaaaac agcagccatc
    21661 gccgccaggg tcgatttgaa catgagattt ccttaacaaa cagagcagaa aaaaagcaag
    21721 tgaagagatt ctaaatagcc ttcaagaaca aggccatccc tttacctaat tttacgtatc
    21781 tgaaatatat gtaacctgac gatcactttt tattccgtga tcaaaatcac ctcttaaaat
    21841 gcaatttagc aaccgattgc aataaaacat ttaaacagat cacaaaatca cctaaaatcg
    21901 cccgctcgcg gcatatcccg ctatgccatt ttatttttga acgcaggcca atcatgacta
    21961 acagcaatcg catcaagctc acatggatta gctttctctc ctacgcactg actggtgcgt
    22021 tggttattgt caccgggatg gtgatgggaa atatcgccga ttatttcaat ctgcctgttt
    22081 ccagtatgag taacaccttc accttcctca acgccggcat tttaatctct atcttcctca
    22141 acgcctggct gatggaaatc gtcccgttga aaacgcagtt acgttttggc tttctcctga
    22201 tggtgctggc ggttgccggt ttgatgttca gccacagcct ggcgctgttc tcggcggcga
    22261 tgttcattct cggggtggtc agcggcatca ccatgtcgat tggtacattc ctggtaacac
    22321 aaatgtatga agggcgtcag cgcggttccc gcctgttatt taccgactcc ttcttcagta
    22381 tggctgggat gattttccca atgatcgccg cgtttctact ggcgcgcagc attgagtggt
    22441 actgggttta tgcctgcatc gggctggtgt atgtcgctat ttttattctg accttcggct
    22501 gtgagttccc ggcgctgggc aaacatgcgc caaaaacgga tgctccggta gagaaagaaa
    22561 agtgggggat cggcgtactg tttctctctg ttgcggcgct gtgctacatc ctcggtcagt
    22621 taggttttat ctcctgggtg cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg
    22681 cgggcacgct ggtgagtaac ttctggatgt catacatggt cggcatgtgg gcgttcagct
    22741 ttattcttcg cttctttgat ttgcaacgca ttctgaccgt actggctggt ctggctgcga
    22801 ttctgatgta cgtctttaac accggaacac cagcacatat ggcgtggtca attctcgctc
    22861 tgggcttctt ctccagcgcg atctatacca ccatcatcac cctgggttca cagcagacca
    22921 aagtaccgtc gccaaaactg gttaactttg tcctgacctg cggaaccatc ggtactatgt
    22981 tgacctttgt ggttaccggc ccgattgttg aacatagcgg tccgcaggcg gcactgctta
    23041 cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc
    23101 gtcaccgtca gcataacacc ctgacctctc attaattgct aatgccggac ggcactatcg
    23161 tcgtccggcc ttttcctctc ttcccccgct acgtgcatct atttctataa acccgttcat
    23221 tttgtctatt ttttgcacaa acatgaaata tcagacaatt ccgtgactta agaaaattta
    23281 tacaaatcag caatataccc cttaaggagt atataaaggt gaatttgatt tacatcaata
    23341 agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga
    23401 gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc
    23461 ttcttgataa atctgatgcg gccaactttg atattaccgt tttctgtgaa gaaccgcgca
    23521 tcgcttatga ccgcgtacac ctctcgtctt acttctctca ccacaccgcc gaagagctgt
    23581 cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa agttctggtc ggcgaacgcg
    23641 ctatcaccat caaccgtcag gagaaggtga ttcactccag cgccgggcgt accgtttttt
    23701 atgacaagct gatcatggcg accggttcct acccgtggat cccgccaatc aaaggttctg
    23761 atactcagga ctgctttgtc tatcgcacca ttgaagacct caacgccatt gaatcctgcg
    23821 cccgtcgcag caaacgcggt gccgttgttg gtggcggcct gttaggtctg gaagccgctg
    23881 gcgcgctgaa aaacttaggt attgaaaccc acgttatcga atttgcccct atgctgatgg
    23941 cggaacagct tgatcagatg ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg
    24001 tgcgcgttca caccagcaaa aacacccttg agattgtgca ggaaggtgtc gaagcgcgta
    24061 aaaccatgcg ttttgccgac ggcagcgagc tggaagttga ctttatcgtc ttctctaccg
    24121 gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg
    24181 gtatcgtcat taatgattcc tgccagactt ccgatccgga tatctacgcc atcggtgaat
    24241 gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc tggctacaaa atggcgcagg
    24301 tcgccgttga ccatattctc ggtagcgaaa acgcctttga aggtgctgac cttagcgcca
    24361 agctgaaact gctgggcgta gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg
    24421 gcgcacgtag ctacgtttac ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca
    24481 gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg cgataccagc gactacggca
    24541 acctgctgca actggtgctg aatgctatcg agctgccgga aaacccggat tctctgattc
    24601 tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt tgataaactg ccggacagcg
    24661 cgcaaatctg ctcctgcttc gacgtcacca aaggtgatct gattgctgcc atcaacaaag
    24721 gctgccacac cgttgcggcg ctgaaagctg aaaccaaagc gggtactggc tgcggtggct
    24781 gtatcccgct ggttactcag gtactgaacg cggaactggc gaaacagggc atcgaagtta
    24841 acaacaacct gtgcgaacac tttgcttatt cgcgtcagga actgttccat ttgatccgcg
    24901 ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg
    24961 aagtttgtaa accgaccgtc ggttcgctgc tggcctcctg ctggaacgaa tacattctga
    25021 agccggaaca tactccgctg caggattcta acgacaactt cctcgctaac atccagaaag
    25081 acggcaccta ctcggtgatc ccgcgttctc cgggcggtga aatcaccccg gaagggctga
    25141 tggcagtggg tcgtatcgcg cgtgaattta atctctacac caaaatcacc ggctcccagc
    25201 gcctggcgat gtttggcgca cagaaagacg atctgccgga aatctggcgt cagctgattg
    25261 aagctggctt cgaaaccggt catgcctatg cgaaagcact gcgtatggcg aaaacctgcg
    25321 tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt cggcctcggc gtggaactgg
    25381 aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta
    25441 cccgtgaatg ttcagaagct cagggtaaag acgtgggtat tatcgccact gaaaaaggct
    25501 ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg tcatgcggat ctgctggcag
    25561 cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg cttcatgatg ttctacatcc
    25621 gtaccgccga caaactgacg cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg
    25681 attacctgaa agcggtgatc attgacgaca aactggggct gaacgctcat ctggaagaag
    25741 agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac tgaaacggtc aatacaccgt
    25801 ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa gcgtgacccg aacgtgcaga
    25861 tggtgccaga gcgcgaacag caccgtccgg caacgccgta tgaacgtatc ccggtaactc
    25921 tggtggagga caacgcatga gccagtggaa agacatctgc aaaatcgatg acatcctgcc
    25981 tgaaaccggc gtctgcgcgc tgttaggcga cgagcaggtc gcgattttcc gcccgtatca
    26041 cagcgaccag gtgtttgcga tcagcaacat cgatccgttc ttcgagtcca gcgtgctgtc
    26101 acgcgggctg attgcggaac accagggcga gctgtgggtc gccagcccgc tgaaaaaaca
    26161 gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa cagttttccg tcaaacatta
    26221 cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt taatgtttta acgggaggcg
    26281 caatgcctcc cctttttgca tggtcctgta atgaccttcg gtataatgca ggacattttt
    26341 taaacttttt gttttatttt ttgtttttat tttttaaagg ataatcaaat gtttacagac
    26401 actattaata agtgtgcggc taatgctgcg cgcattgcac gcctgtcggc aaataacccg
    26461 ctcggctttt gggtcagctc cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg
    26521 attttcacgc tcggtaattt gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc
    26581 tttggtatcg ccttaacgct ggtgattatc gccggttctg aactgttcac cggacacacc
    26641 atgttcctca cctttggggt aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc
    26701 ctgccgcaaa cctggctggg taacctggtc ggttccgtct tcgttgccat gctctatagc
    26761 tggggcggcg gtagcctgct gccggtagat accagcatcg ttcactccgt cgcgctggct
    26821 aaaaccactg caccggcaat ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt
    26881 tgcctggcaa tctggatggc gctgcgcact gaaggggcgg cgaaatttat cgctatctgg
    26941 tggtgtctgc tggcatttat cgcgtccggc tacgagcact ctatcgctaa catgacgctg
    27001 ttcgcgctct cctggttcgg caaccacagc gaagcctaca cgctggcggg tattggtcat
    27061 aacctgctgt gggtgacgct gggtaatact ttatcaggtg ccgtattcat gggattgggt
    27121 tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa
    27181 acggctgccg gttaattact aaggggtttt tacgtggatc atttgcctat attttgccaa
    27241 ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg
    27301 ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat tagcgtttat tccacagttc
    27361 accgcatggg cagatgcagg catgttaacc ctcgtcgaag ggccatttga tgaaagcctt
    27421 ctcgacacct gctggctggc gattgcagcg acggatgatg acacgcttaa ccagcgcgtc
    27481 agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc
    27541 agctttatta tgccgtcgat tattgaccgc tcaccgctaa tggtagcggt ctcctctggc
    27601 ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg
    27661 catctgggcc aggtggcgaa atatgccggg caattacgcg ggcgagtgaa acaacagttc
    27721 gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg
    27781 cagtcgctgg caaacaacga tcagaaagcc attactgaaa cgaccgaaca gttaatcaac
    27841 gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg caggtccggg cgatgccggg
    27901 ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt
    27961 ctggtttctg acgatattat gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc
    28021 aaacgcgcgg gataccactg tgtaccgcag gaagagatta accagatcct gctgcgggaa
    28081 gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg atccgtttat ttttggccgt
    28141 ggtggcgaag agctggaaac actgtgcaac gcgggtattc cgttctcggt ggttccgggt
    28201 attaccgcag cttctggttg ctctgcctat tcgggtattc cgctcacgca tcgcgattat
    28261 gcccagagcg tgcgcttaat taccggacac ttaaaaaccg gtggcgagct ggactgggaa
    28321 aacctggcgg cagaaaaaca gacgctggtg ttctatatgg ggctgaatca ggctgcgact
    28381 attcagcaaa agctgattga acacggtatg cctggcgaaa tgccggtagc aattgtcgaa
    28441 aacggaacag cagtcacgca gcgcgtgatt gacggtacgc tcacacaact gggcgaactg
    28501 gcgcagcaaa tgaacagtcc atcgctaatt attattggtc gggttgttgg cctgcgcgat
    28561 aaactgaact ggttctccaa ccattaattt aacccgggcc agagaattct ggccttctta
    28621 atgactcttt ttatatattc agcaaataaa acataacccc ttaataaata ttatctgcta
    28681 atgggttata ttcaccgtta cagcgactta caaaattaag tcattcgctc actgacgcca
    28741 attaaatatt cctgtatcgt tcttaacgag cagtccgctt atattcatat aatcaatgaa
    28801 tattaattaa tatataatac atagggaatg taaatgaaca aatttattaa agttgcactg
    28861 gtaggtgcag tactggctac gttaactgca tgtactggtc atattgaaaa ccgtgataag
    28921 aactgctctt acgactacct gctgcacccg gcaatttcta tttctaaaat cattggcggt
    28981 tgcggtccta ctgcacagta agtctctgac agaaatcggc taacaccggt ttaattgccc
    29041 gacgccctct ggattttccg agggcgtatt tgtttctatg tccttcttgc ccccgcccgt
    29101 aaaaatattt tcactttaaa ttcaatttga taactacatc cattagccac agcgcgatcc
    29161 tgtgcgaaat tttgtgatct tcctccacat tacataacat catatgttgt tatattcatc
    29221 atgtattgtc atgttacctt ttaaatgact gcaaactctc ccctacaacg tattggacaa
    29281 gaaaaaggta tcgctatggg aagccaggaa ctccaacgca agctcggatt ttgggccgtt
    29341 cttgcaatcg ccgtcgggac aaccatcggc tccggtattt ttgtatctgt gggtgaagtg
    29401 gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt
    29461 gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg cttatccgga aaatggcgca
    29521 gattatgttt atctgaaaaa tgccggaagc cgaccgctgg ctttcctctc cggctgggcc
    29581 agcttctggg ccaacgatgc gccgtcattg tcgattatgg cgctggcgat tgtcagcaat
    29641 cttggctttt taacgcctat cgatccgttg ctcggtaaat ttatcgccgc cggattaatt
    29701 atcgccttta tgttgctaca cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta
    29761 attaccatcg ccaaaattat cccgttcact atcgtcattg gccttgggat cttctggttt
    29821 aaagcggaga attttgccgc ccctaccacc actgcgattg gcgcaacggg cagctttatg
    29881 gcgctgctgg cggggatctc tgccaccagt tggtcgtata ccggcatggc ctctatctgt
    29941 tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc cacgagcgct gattggttcc
    30001 tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg tgatttccgg cctgatgccc
    30061 ttcgacaaac tcgccaattc tgaaacgccg atttccgacg ccctgacctg gatccccgca
    30121 ctcggcagca ccgctgggat ctttgttgcc atcatggcga tgatcgtcat tcttggttcg
    30181 ctttccagct gcgtgatgta ccagccgcgg ctggaatacg cgatggcgaa agacaacctg
    30241 ttctttaaat gcttcggcca tgtgcatccg aaatacaaca cgccggatgt ctccatcatc
    30301 ctgcaagggg cgctggggat cttcttcatc ttcgtttccg atctcaccag cctgctgggt
    30361 tatttcaccc tggtgatgtg tttcaaaaat accctcacct tcggctccat catctggtgt
    30421 cgtaaacgcg acgattacaa accgctgtgg cgtactccgg ctttcgggct gatgaccacc
    30481 ctcgccattg cgtcaagcct cattctggtc gcctcaacct ttgtctgggc accgattccc
    30541 ggccttatct gcgccgtcat cgttattgct actggtctgc ctgcttacgc cttctgggcg
    30601 aagcgtagcc gccagctcaa cgctttgtcg taatgttatc tggagaaaat aaaatgttgg
    30661 atattgataa aagcaccgtg gactttctgg tcaccgaaaa tatggttcag gaagtggaaa
    30721 aagttctcag ccatgacgtt ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg
    30781 acattgatcg tatttatttc gttgcctgcg gctcgccact caacgcggcg caaacggcga
    30841 aacatctggc ggatcgcttt tccgatcttc aggtctacgc catttccggc tgggagttct
    30901 gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt gattggcgtt tctgactacg
    30961 gtaaaacgga agaggtaatc aaagcgctgg agctgggccg ggcctgcggc gcactcactg
    31021 cggcgttcac caaacgcgcg gacagcccga ttacctcggc ggcggaattt agcattgatt
    31081 atcaggccga ctgtatctgg gaaattcacc tgctgctctg ctacagcgtg gtgctggaga
    31141 tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat caaaaacgat ctcaagcagt
    31201 tgccgaatgc gctcggtcat ctggtacgca cctgggaaga aaaaggccgc cagcttggtg
    31261 aactggccag ccagtggccg atgatttata ccgttgctgc gggtccgctg cgtccgctgg
    31321 gttacaaaga aggcattgta acgctgatgg aatttacctg gacgcacggc tgcgtgattg
    31381 agagcggaga gttccgccat ggcccgctgg agattgtcga accgggcgtt ccgttcctgt
    31441 tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg cgccattaac tttgttaaac
    31501 agcgtactga caacgtgatc gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt
    31561 ggctggcacc gttcctgatg ttcgtgccaa tggagtggct ctgctactac ctgtctattt
    31621 acaaagatca caacccggat gaacgccgct attacggtgg tctggtggaa tattaatccc
    31681 tctcccggcc cggcaacggg ccgaatttac gcaaggagtt acccgatgaa aacaggtatg
    31741 tttacctgcg gccaccagcg gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg
    31801 ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa
    31861 gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt atcagatgcc gattatcggc
    31921 tatacgcccg aaaccaacgg ctatccgtat aacatgatgc tgggcgatga acatatgcgt
    31981 cgcgaaagcc tcgacatgat caagctggcg atggatatgg caaaagagat gaacgcgggt
    32041 tatacgctga tttccgcggc ccacgcgggc tatctcacgc cacctaatgt tatctgggga
    32101 cggctggcag agaacctaag cgaactgtgt gagtacgcgg aaaacatcgg catggatcta
    32161 atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt
    32221 catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg
    32281 tatgtccagg cggaaccggt gatgagttat ttcgacaaac tgggcgataa attacgtcat
    32341 ctgcatattg tcgacagcga cggggccagc gacacgcatt acattcctgg agaaggcaaa
    32401 atgccgctga gggaactgat gcgcgatatt atcgaccggg gctatgaggg ttactgtacg
    32461 gtggagctgg tgacgatgta tatgaacgag cccagactct atgcccgcca ggcgctggaa
    32521 cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac cctggcgaca atcggcgata
    32581 actgcgtcga tatctacccg caactgaata aagcgttttc tggcggtaat gcggtcaatg
    32641 tggcggtgta ctgcactcgc tacggcatac agccgggatg cattacctgg gtgggtgacg
    32701 atgactacgg cacaaagctg aagcaggatc tcgcccgcat gggcgtcgat atcagccatg
    32761 tccatacgaa acacggcgtt accgcacaaa ctcaggtgga actgcacgac aatgatcgcg
    32821 tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc cctgagtgaa gaggattacg
    32881 cctggctggc gcagtatgac attgtgcacg cggcaatctg gggacatgcg gaagacgcat
    32941 tcccacagct gcacgctgcg ggcaaactta ccgctttcga cttctccgac aagtgggaca
    33001 gctcgctctg gcagacactg gtgccgcatc tcgattttgc ctttgcctcc gcaccgcaag
    33061 aagacgaagc gctgcgtctg aagatgaaag cgattgttgc ccgtggcgca ggcgtggtga
    33121 ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg cgcgcagttc tggcgtcagg
    33181 ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg agattcgttc attgccggat
    33241 tcctctgcgg ctggtctgcg gggatgacat taccgcaggc gatggcgcag ggaacggcgt
    33301 gcgcggcgaa aaccattcag taccacggtg cctggtaggt ataacgttgg cgtgagcatc
    33361 ttcacgccaa cgtgctgtta cttgccggaa aacgacccta taatccgagt aattcattct
    33421 ttatttcagg gtcgattatg tcagctacgg accgctactc tcatcaactc ctctacgcta
    33481 ccgtccgcca gcggttgctg gatgatatcg cgcagggggt ttaccaggcc gggcaacaga
    33541 tccctaccga aaacgagctt tgtacgcaat ataacgtcag ccgcattact attcgcaaag
    33601 ctatcagcga cttagtggca gacggcgtac tgatccgctg gcagggcaaa ggcacctttg
    33661 tacaaagcca gaaggtcgaa aacgccctgc tgaccgtcag tggttttacc gattttggcg
    33721 tctcacaagg caaggcgacg aaagagaaag tgatcgagca ggaacgagtc agcgccgcgc
    33781 cgttttgcga aaagctgaac atccctggca acagcgaagt gttccatctt tgccgggtga
    33841 tgtatctcga taaagagccg ctgtttattg atagttcctg gatcccactg tcgcgttatc
    33901 ctgactttga tgagatttac gttgaaggaa gctccactta tcagttattt caggagcgtt
    33961 tcgacacgcg agtggtcagc gacaaaaaga ccatcgatat ctttgccgcc acccgcccgc
    34021 aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt tcgcatcagc aaaatcgcct
    34081 ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt ctgccgcgcc aatcgcatca
    34141 ccttaacgat tgataataaa agacattaac cgtaggccgg ataagatgcg ccagcatcgc
    34201 atccggcgat gctggcgcgt tgaattttac atcccgtacg ttcccctcac cctaaccctc
    34261 tccccaaagg ggcgagggga ccgttcagta cggaatttag cttgacggtg ctatccagca
    34321 ttaaatactg gcaatactct cacgcaaaat ccgcagcacc gtctcttcac cgctgcgatt
    34381 cgggttaatg cgaatcgcac aatgttctga ttgtggattc gcctggcgaa acgttccgga
    34441 aaggcgataa aagagcggcg ggatttcata tttcgactct gcacccaccg ggtaaggcaa
    34501 ggcaccgcgc ttttgcgcct cttccagcac tctggcggca atcggctgat gaaactcgac
    34561 aatcaacacc ttcgactgcg cattagcaat caccgcgctt ttcacttccg gcaccgcacc
    34621 accgttaagc aaagccagca accgttcaga tacccctgcc tgcaccgcgt gcatcactgg
    34681 cgcaaacacc agaccacgca atacttccag cgcctgtgcg ccctggatct ggctaccgcc
    34741 ggagtaaagc gtggcgcgaa tacggttgat aacatcagca tcgccgacca ctgcaccaac
    34801 accctctggc ccaaatagct tgaagcagga aaatgtcgag acattcgcgc cgcattcaca
    34861 gccgattcgc gccaccttca tcaccgcata gttgtcatcg gttaacgctg gaacacctgc
    34921 cgcgcgcaac gttgccagca catctgccag cacgtagctg tcctgcggct gctggcgcgt
    34981 atgctgcacc agcgccgcat ccggttgttg ctcgtcgacg acctgcttca gtgccgacag
    35041 gtcattgaaa tcaacagtaa taagcgtcag ccccatctgc tcaataataa cccgtgtcgt
    35101 cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc ggtttgagca aagccgccag
    35161 cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc gccgcagcct gtgcgtgaaa
    35221 tgcatcagcc agcacctgct ccacccgttg ggtaatacgc ggttgattca gacctggagt
    35281 taaccctaaa tcaccgccag taagaaactc gctgccgggg aaatggcgac aaatgctatc
    35341 caccagcgca aactgctttt gctgcgcctc aataatcgtc aggctttgca gaggaaacgt
    35401 cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag aacattcagc
    35461 agaataccgg tgatcattac tgccacgacc ggtgccgcca ttttctgtac cggacggccc
    35521 agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata acccgccatc
    35581 ttaattgctg cgaaaatcga accgaccagc agcgccactt ccatcagcat attcatggcg
    35641 ttacggatgt tatccgacgc attacgcacc gacgggtagc gtcccagcca tttgccgatc
    35701 gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc cgcaaccatc
    35761 ggatttggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac tgcatacacg
    35821 ccagtggcta acgcggtggt ggcaatcaac ggcacaaaac ccagtccgcg cataaattct
    35881 gccagagccg cctgattaat cagcgtttgc gattgttccg gcgtcacgcc tgcggaatac
    35941 gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa aaatcttcat actggcaacg
    36001 gcggcaatca atgctcccac gatggcgata taaggtaagt ttttgataat ccgcgacgtg
    36061 cgctcttcaa acaccgaaag cccgctggca tcgatgtcat tttcatcacg atgacgcagg
    36121 tcgtgagtta tcgcgatccc cagcagcatc accatgccaa taaagatttc gatggattca
    36181 gggttaagat gtgggaaata gcgcacgact accacacggg tcatcagaac aaccacggcg
    36241 gcgaccagac tttgcttcca gccaaactgg tagaaaatcg ccaccagcgg gaacagtgca
    36301 aaagcagaaa ccaccggcga gcttaattcc ccgaggctac ctaatacatc caccggcagc
    36361 gcggtcagca gctggtttac tggcaacagg caagtaagga tcaacacgcc ccagatagcg
    36421 ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc ggtaggaaga
    36481 aagagtaacc atgcgttgag cagcccggtt ttcagggtaa aagagatccc caccgaggcc
    36541 acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacgatt catatagcct
    36601 tcaatcagtt gcggcaggat cgggcggatg ccgtcatgaa aaacagccgc cgagcgatgc
    36661 gccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat atacagatcc
    36721 atagtcgtgc cttttatttc agatggttaa tcagcatcgg aatcgcgtgt tcgacgtgct
    36781 caacggaaag gccaaacgcc actttccctt cagcgatcat tttggcgata tgctcgtctt
    36841 tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc accgcgatgg
    36901 caatcgacaa tgccgcgccc gcaccggtat tacacgcacc gatgtagtaa tccagttgcc
    36961 cggatttcac tttcattgcc gcttccatgt cgttgtgaat gaaaacttca aaactgccag
    37021 gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca acgccaatct
    37081 ttttcatgag tctgtcctta ttggaaaaat tgagagggat tttcacgtaa catcacatcc
    37141 acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaggtggt caataaatag
    37201 tcatagccat aaccaccatt ggcttttaaa tgggagcggc gcgtaatatc catcgacagc
    37261 atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga gcatcgcaat gcgcttttcg
    37321 tccgggtagt aactgttctt accaatggtg tcgaactgca cgtacgcgcc gagatcgatc
    37381 atcttcaaaa tgttgtcgag attgtctttc agatcgcagt gaccaacggt gacgcgcgaa
    37441 agatcaactc cgtgggcttg caacagagcc agttgttcca gccccatggt gctgaacgaa
    37501 gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcagcggc aataaacacc
    37561 ttctcttcca gcggcgtaat cttcccttcg ctagagccga tctccgcgat aatcccggct
    37621 ttcaggtcgg tgccatcgat accctgttca atttcatcga ccatctcctg cgccagttcc
    37681 tgcacgctgc gggtcgccac atgttccggg aaaaacgcgt cctggtaata accggtacag
    37741 gccaccacgt tgatccccgt ctcgcgcatt acatcaagca taaattgcgc attgcgcccc
    37801 atgtaacggt tggtcatctc aatcacatta cgcacgcccc gggtcatcag gtcgttcatc
    37861 tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt aaagccggag
    37921 agatcaatat gcagatgctc atgggcgagg gtgtaacccg tcggatcaaa actcataggg
    37981 tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc ggtggcgcgc
    38041 ggaaaaattc acacacggta gcccccacat cagaaagcgt ggtgcgcaca ccgagctgcg
    38101 tagcgatcat cccttgctga taaaccagca ctggcaccac ttcgcgggta tggtggctgt
    38161 gaccaatggt cggatcgtta ccgtgatccg ccatgacgat caggcagtca tctggctgca
    38221 tcgcctcaac aagccgggca aggttacggt caacgacctg caaacgttcg gcataacgtg
    38281 cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attggtgcaa ataaacgccg
    38341 tcggatgcgt gttaaattcg tcgagggtga tatccataat ccgctggcta tccaccaggt
    38401 tttgccagct cacgccataa ggattgctga cgatatctgc caccttaccc accagcacgg
    38461 ttggcacgcc cgcctcatac agtttttgcg gcacctgcac tttttcatca acaccgtagc
    38521 ccatatgcac gacctggaaa ccgttgtcat aagcgccaga acgcggcgca ttaataccaa
    38581 taaagcgccc ttctttgctt tctgcggcat cgagaatacg ctgactgttg gttaacaggc
    38641 cgccaaatgt aatgacccga ccgacctgta cctgctcacg cacgatacga ccaattttga
    38701 ttgcgtcgtc aaaagagatc acagagagat tggcggtaat gttatagact tggcctaaat
    38761 ccgcctcgag attatcgcca atcgcaaccg cctgattgac ccacagaaat tgcagttcat
    38821 cgccacggcg ctccacctgc caaccggcgg atactaatgc ctgctcaaca cggtcaatca
    38881 catcgcaaaa aggcatccgc agcggcggta acgggcgcgt gcctaaaatt tcctgatgcc
    38941 ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt gcggaatctg
    39001 acggctgcat atcgcctggc gcataaccca atgcgttgat tagccccagc ttctccagcg
    39061 ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattc gctcccgcat
    39121 cttgcggacg caccagcgtg acatctttca ttgcccctac gccaaagcta tcaatcacta
    39181 acaccacaaa tcgcgccatt acgcccccag agagtttcca agactgtcat aacgaccaac
    39241 gattttcggt tcgccacggt gaataccgga caccagcacc acatcgctac gggtgacaaa
    39301 aatctgcgtg cggaaacaga gcaccactgc actgctcacc ggatactcgc ctgccagcgg
    39361 cagggtgtag tcgatactgc tgtcatccac agttttgaga ttggtttcag taatcttttg
    39421 attttctggc gtaaacacca gcgcatgttg cgcatgacca cgacggtagt aaccgccgcc
    39481 gtagcagtag ctgtcgccac ggaaatgatg ggagatttca cttaaccaga gcatcgcgat
    39541 acgttcaggc tgatcgccct gctggtttgc cggaatagtg cccgtcagcg catgaccggg
    39601 ttcggcatga gtcacaccgt attccgccag caatggcagc gaagtgcagc tggtcgctga
    39661 aggcgcgttc agttgctcaa gtgcaatacc agatttcgcc agttgatccc gtgcctgtat
    39721 cagcgtgtga agattcggtg tcggcaaaac ttttccggca gcctcatccc aaagcaggca
    39781 agggaaatgg gtaagtccgg ctaaatgcag ccccggcagt ttcttaattt cagcaaccac
    39841 ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc ggataaagaa aatcatcgtc
    39901 gctataaact ttgagcagca caaactgcac acgtccggcc ttcaccgctg ccgcagaaac
    39961 ttcccgcgct ttgtcgagag taaacacggt gatgacgtcg gtgccctgtt caacggcgtc
    40021 agcaacctga tgacaaggga tttgtaccag atgcccctga tgcgccaccg acaaaccagc
    40081 gcggcgcatg actcgcgcct ctttgtaatc caccgccaca atgccgctgt aacctaatgc
    40141 cagcaatttt tccgccagcc atgggttacg gccaaactgc ttggtcatca gatacagttc
    40201 aatgccgtaa agccgcgccg tctcaatcag tcgcttaccg ttttccagta cctgatccac
    40261 gtcgatcacc cagctgtccg gggcgatctt gccctgctgc cacaaactta gtgcggcgga
    40321 aatcagcgcc gggttctggc gtttcaatgc ttctacaaac atcttgttgc ctcgtttctg
    40381 ttccttgaat taaatattca tttttttgaa tatttaggtt gtgcgacgtg gatttgtcgg
    40441 gagcttcaaa cctcttcgtt agcagccatc cataatccat aaaggttggc cagcaaatag
    40501 ccttcttcac acgggttaac ttccagtgcg aattccttca acaacacctg atgcaattgc
    40561 acaacggctt gccagtggct ggattgcgcc agctctgcca gcaattcgtt atccagcggc
    40621 tctatttctt caccacggcg actgcgcatc agtgcactcg ccatatgtgt catcgccatc
    40681 gttccttgct cactgcgcac cggcagatgg cactctgttt ccagtacgtt gacgacctgc
    40741 atcatgcctt tgcagatgtc cttatcaata acgcctgcct cgcaaagcag gttgagtctg
    40801 gtttccatat cagatgcctt tcgtttaata actcggttcc tgttccccac tcactacccg
    40861 ttgttgatgg gcgagcaggg cgtgtttatc tacaacggca cgcagcagtt gttgcattgg
    40921 gtaatcatcg actcgcgtca ggaccacggc ttcggtggcc tgtaaaaatc gcggatcgtc
    40981 tgtgagcggc gtcgcctcta atcccagcat ggtcagttcg ttttccgcca ccacgttcca
    41041 gatcaccgca tcgacatcgc ctttaacaat gcgttgtaaa ctctcgtgat aagagagatc
    41101 gactcgttcc acatcactac cgccaaaaaa aacatcggtc atgattttct gatccgccga
    41161 acggttatcc agccccacgc gcttcacgtt tgcggactcg cctttacggc aaatcaactg
    41221 gtgctcgcca acgtaggtgt gcggccccaa ctccagcgcg aggcataacc ctttttgcgt
    41281 gagataactt tccgccgcca gtcgggaaac caccgccatg tcatacacgc cgttaagtaa
    41341 acactccacg cgaatatccg cgccgcgcat atgcgcatag taaaaaggaa tgccatcaaa
    41401 ctgagctttc agcccgctcg ctaaaccttc gtacaagcgg gtatagggca aaggcatcgc
    41461 acataccacg ttgttgatat ccacatgact cagcaatgct ttgttatcca tctcgaccag
    41521 ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct ccgcttgatt ccagcgtttt
    41581 caacgcggcc tgcgtcaggc caaccgatga acggcattca gttgccagct catctatggt
    41641 tttcaaccta ttaccgcatt tttcacccaa caaataacgg gccagcgtcg tgataacgac
    41701 gccttctttt ttga
//
LOCUS       DAPPNJ010000011        41265 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000011 DAPPNJ010000000
VERSION     DAPPNJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.011"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(14..1411)
                     /gene="ccp"
                     /locus_tag="RPM73_000449"
     CDS             complement(14..1411)
                     /gene="ccp"
                     /locus_tag="RPM73_000449"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDX6518844.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1816..3465
                     /locus_tag="RPM73_000450"
     CDS             1816..3465
                     /locus_tag="RPM73_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDX6518845.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILILYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3516..4118)
                     /locus_tag="RPM73_000451"
     CDS             complement(3516..4118)
                     /locus_tag="RPM73_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX6518846.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHCNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4638..5537
                     /locus_tag="RPM73_000452"
     CDS             4638..5537
                     /locus_tag="RPM73_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6518847.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5586..6599
                     /gene="yhjD"
                     /locus_tag="RPM73_000453"
     CDS             5586..6599
                     /gene="yhjD"
                     /locus_tag="RPM73_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDX6518848.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7010..8332
                     /locus_tag="RPM73_000454"
     CDS             7010..8332
                     /locus_tag="RPM73_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDX6518849.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8566..10626)
                     /locus_tag="RPM73_000455"
     CDS             complement(8566..10626)
                     /locus_tag="RPM73_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDX6518850.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10696..11463)
                     /gene="pdeH"
                     /locus_tag="RPM73_000456"
     CDS             complement(10696..11463)
                     /gene="pdeH"
                     /locus_tag="RPM73_000456"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDX6518851.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11695..12624
                     /gene="kdgK"
                     /locus_tag="RPM73_000457"
     CDS             11695..12624
                     /gene="kdgK"
                     /locus_tag="RPM73_000457"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDX6518852.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12720..14216)
                     /locus_tag="RPM73_000458"
     CDS             complement(12720..14216)
                     /locus_tag="RPM73_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDX6518853.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14437..15723)
                     /gene="dctA"
                     /locus_tag="RPM73_000459"
     CDS             complement(14437..15723)
                     /gene="dctA"
                     /locus_tag="RPM73_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDX6518854.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15906..17894)
                     /gene="hmsP"
                     /locus_tag="RPM73_000460"
     CDS             complement(15906..17894)
                     /gene="hmsP"
                     /locus_tag="RPM73_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDX6518855.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENVMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17976..21449)
                     /gene="bcsC"
                     /locus_tag="RPM73_000461"
     CDS             complement(17976..21449)
                     /gene="bcsC"
                     /locus_tag="RPM73_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDX6518856.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21431..22537)
                     /gene="bcsZ"
                     /locus_tag="RPM73_000462"
     CDS             complement(21431..22537)
                     /gene="bcsZ"
                     /locus_tag="RPM73_000462"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDX6518857.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22544..24883)
                     /gene="bcsB"
                     /locus_tag="RPM73_000463"
     CDS             complement(22544..24883)
                     /gene="bcsB"
                     /locus_tag="RPM73_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDX6518858.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24894..27512)
                     /gene="bcsA"
                     /locus_tag="RPM73_000464"
     CDS             complement(24894..27512)
                     /gene="bcsA"
                     /locus_tag="RPM73_000464"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDX6518859.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27509..28261)
                     /gene="bcsQ"
                     /locus_tag="RPM73_000465"
     CDS             complement(27509..28261)
                     /gene="bcsQ"
                     /locus_tag="RPM73_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDX6518860.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGGQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28273..28461)
                     /locus_tag="RPM73_000466"
     CDS             complement(28273..28461)
                     /locus_tag="RPM73_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDX6518861.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28734..30305
                     /gene="bcsE"
                     /locus_tag="RPM73_000467"
     CDS             28734..30305
                     /gene="bcsE"
                     /locus_tag="RPM73_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDX6518862.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30302..30493
                     /gene="bcsF"
                     /locus_tag="RPM73_000468"
     CDS             30302..30493
                     /gene="bcsF"
                     /locus_tag="RPM73_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDX6518863.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30490..32169
                     /gene="bcsG"
                     /locus_tag="RPM73_000469"
     CDS             30490..32169
                     /gene="bcsG"
                     /locus_tag="RPM73_000469"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDX6518864.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32256..32363)
                     /locus_tag="RPM73_000470"
     CDS             complement(32256..32363)
                     /locus_tag="RPM73_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX6518865.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32739..32846)
                     /locus_tag="RPM73_000471"
     CDS             complement(32739..32846)
                     /locus_tag="RPM73_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX6518866.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33222..33329)
                     /locus_tag="RPM73_000472"
     CDS             complement(33222..33329)
                     /locus_tag="RPM73_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX6518867.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            33805..35076
                     /locus_tag="RPM73_000473"
     CDS             33805..35076
                     /locus_tag="RPM73_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDX6518868.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(35106..36110)
                     /gene="dppF"
                     /locus_tag="RPM73_000474"
     CDS             complement(35106..36110)
                     /gene="dppF"
                     /locus_tag="RPM73_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDX6518869.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(36107..37090)
                     /gene="dppD"
                     /locus_tag="RPM73_000475"
     CDS             complement(36107..37090)
                     /gene="dppD"
                     /locus_tag="RPM73_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDX6518870.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(37101..38003)
                     /gene="dppC"
                     /locus_tag="RPM73_000476"
     CDS             complement(37101..38003)
                     /gene="dppC"
                     /locus_tag="RPM73_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDX6518871.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(38013..39032)
                     /gene="dppB"
                     /locus_tag="RPM73_000477"
     CDS             complement(38013..39032)
                     /gene="dppB"
                     /locus_tag="RPM73_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDX6518872.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(39340..40947)
                     /gene="dppA"
                     /locus_tag="RPM73_000478"
     CDS             complement(39340..40947)
                     /gene="dppA"
                     /locus_tag="RPM73_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDX6518873.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         9595 a        11354 c        10406 g         9910 t
ORIGIN      
        1 gcgatcaaat taattattgt ttatcctgca tatacggcgt gtacaccccg ttcagactgt
       61 gcaggaaagc tacgatatca tccacatcct cctgcggcag ctctttgcct acctgatagc
      121 gcagcatcag tttcaccgcc ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg
      181 ccgttaaagc aacgttacgt aaaccgggta ctttctgacg caatttatca cgctcttctt
      241 tagtcacatt catacgacca atatccgccg ccgtaatttc cccaaagtta aagtcttttt
      301 tcagccccaa cggttcaaag gaacgtccgc cgagaataat accaccatga caagttgcac
      361 atttattatc tttaaataat tgatagcctt ttttctgttg cgccgtcagc gcattttcat
      421 ctccacgcaa ccatttatca aatggggaat ccggcgtaat taatgttttc tcaaattcag
      481 caatggcatc agtaatattt tcgccactga aaccttgcgg ataaacttcg aggaactgcg
      541 ctttaagctg cggatctttt tccagcttag caataatttc gtcccaggat ttcgacgcca
      601 tttcaatcgg gttcaacggc ggtccacctg cctgatcctg caatgttgcc gcacgaccat
      661 cccagaactg ctcaacgtta aatactgagt taaataccgt cggcgcgtta atcggcccaa
      721 ctgcgccacc aacaccaatc gatgtttttc tgccatcgac gccccccgca ttcaacgcat
      781 ggcaatgagc gcatgaaatg gtgctatcag ccgataaacg gggatcgtga tacagcgcaa
      841 agcccaacgc cactttttgc gcatcggtag gcagtttttg cgggatgggc tgcaccggtt
      901 cattgcgatg ctccggagca gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa
      961 tccaggccag tatttccgcc cgctcttcat cactcacctt acccgcccag tgtagcgcgg
     1021 tataacgcgt tggtggcata gtttcatact gcatcaccca ttcaatctta ttcagatcgc
     1081 tttgcgaaac gggtttatca gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt
     1141 tatatccaag cttaatgtcg taatccatca actgtttcgc gccaggaata taataatagg
     1201 cgggtaattc tgccgaaggt gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta
     1261 aaaccttatt attttcactg acagcagatg cctgaacatc ggcctgttta ctgcgtttat
     1321 tatcgtggta ccacacataa ccagataacc ctaaatagca aatcgcgacg ccagccaggc
     1381 cgatcgcggt aatacgtgag accattttca ttattcttcc ctcacggtgg tgagttattg
     1441 tgacccagaa aattttccag aatgctgcaa acgtctctgc gagacaaagc catcataaaa
     1501 aacagggaga tacgcttttt gataggaatc aatcaattac atgaatataa actatcaata
     1561 agatagcctg tgcctattac ccagatcagg aatggcatat catgattgcg gcgagagcag
     1621 gattgaatgt tctgaaaatg aaaaacaaca cgaaatccgt atttttataa attccatgga
     1681 tattagtgcg ttatattttt tgacaggtta ataaacgtga tccaccgcac gctttgtcgc
     1741 ccaccaggcg gagcgaatga ctacccttaa agaaaagccc gataattagc gacgaatttc
     1801 ggaggttgga tccttatgct caatcagaaa attcaaaacc ctaatccaga cgaactgatg
     1861 atcgaagtcg atctctgcta tgagctggac ccgtatgaat taaaactgga tgagatgatc
     1921 gaggcagaac cggaacccga gatgattgaa gggctgcccg cctctgatgc gctgacgcct
     1981 gccgatcgct atctcgaact gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt
     2041 aaaacctttc ccgactgcgc acccaaaatg gacccgctgg atattttaat cctctaccgt
     2101 aaagtgcgcc gtcatcgtga ttttgacttg cgcaagtttg ttgaaaatca cttctggctg
     2161 ccggaggtct actccagcga gtatgtatcg gacccgcaaa attccctgaa agagcatatc
     2221 gaccagctgt ggccggtgct aacccgcgaa ccacaggatc acattccgtg gtcttctctg
     2281 ctggcgctac cgcagtcata tattgtcccg ggcggccgtt ttagcgaaac ctactattgg
     2341 gattcctatt tcaccatgct ggggctggcg gaaagtggtc gggaagattt gctgaaatgc
     2401 atggccgata acttcgcctg gatgatcgaa aactatggtc acatccccaa cggcaaccgc
     2461 acctattatt tgagccgatc gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa
     2521 gaagatggtg tacgcggtgc gcgccgctat ctcgaccacc ttaaaatgga atatgccttc
     2581 tggatggacg gtgcagaatc gttgatccct aatcaggcct atcgccatgt tgtgcggatg
     2641 ccggacggat cgctgctcaa ccgttattgg gacgatcgcg acacgccgcg tgacgaatcc
     2701 tggcttgagg acgttgaaac cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc
     2761 gatttacgcg cgggagcggc ctcaggttgg gattactctt cccgttggct gcgtgatact
     2821 ggtcgtctgg cgagcattcg taccacccag ttcatcccca tcgatctgaa tgccttcctg
     2881 tttaaactgg agagcgccat cgccaacatc tcggcgctga aaggcgagaa agagacagaa
     2941 gcgctgttcc gccagaaggc cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat
     3001 gatgaaaacg gcatctaccg cgattacgac tggcgacgcg aacaactggc gctgttttcc
     3061 gctgccgcca ttgtgccgct ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg
     3121 gcaaacgccg tacgcagccg gttactgaca cctggcggga ttctggcaag cgagtacgaa
     3181 accggtgaac agtgggataa acccaatggc tgggcaccgt tacaatggat ggcaattcag
     3241 ggatttaaaa tgtatggcga tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa
     3301 acggtgaatc agttctatct ggaacagcac aaaatgatcg agaaatacca tattgccgat
     3361 ggtgttcccc gcgaaggcgg cggtggcgag tatccgttgc aggatgggtt tggctggact
     3421 aacggtgtgg tacgccgttt aattggtttg tacggcgaac cataatattt ttacagccag
     3481 ccgctaactt cctgctggct gtaaaattat cctcttcagg aggagatatt taacatcatt
     3541 gccgcctggg tgcgattttt cacttccaga cggcgataca gggattccag atgcgctttt
     3601 accgttccgg tactgatatt cagcgctctg ccgatctcct tatttgattc gcccgccgct
     3661 aacatggtta aaatctcccg ctggcgggcg cttaacgatt tgagatcttt aatgtccttt
     3721 tccggcgtcg tccgccagtc tccaggcaga aacatcatcc ccatcgccgc actatttacc
     3781 gccaacgcaa atgtctcgac ggttgaatca cgaggcacaa tggccagcac attacaatga
     3841 ataacttcct gtaaccaccg tttattgcaa tccgtcgccg tgattaacac cttaacctca
     3901 ggaaactgca ccacggtttt ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca
     3961 tcgagcataa ctaaggcttc agggtaactt tccagctttt gccataactc gtctgcctga
     4021 ctggccccct gaatactcac tcctggaata cgctgctgta aactgatttt cattccatga
     4081 ataaatattg actgcctgtc aaacatgact atttgcataa ctgaatctcc acctgaatac
     4141 gttaaaaaga cttaagtagt ggaagggtat tacccgcgag aaaaaataag aattcgccat
     4201 ttggcggtgg ccattctaca gagatgacgc gtagaaaata gttaccgata taaatagtta
     4261 cagctaaacg cctgaaatta catgtcgagg gcactattta aaacaatttt gaggatttcc
     4321 ttatattggt ggttagtacg catgcaatta aaaatgaaac tccgcgacca caagcaaaaa
     4381 taacaaacgg caatgagaca aaaataagca caaatagcca acacgtcctc tattcacttt
     4441 aaagggaatc gctgaaaaat acgctctgtt tagggggatt cacctttctc agaaagctat
     4501 tccgcccttt tcctgctgag aaatcgccac attcgtcatg acaacattgt gaaacccggc
     4561 attagatgtt agaaaaaaca ataaacaatg cgatatgcgc gttactgttt cgctgtgaac
     4621 aacaatcggt caaagaaatg gataaaattc acgcaatgca gttgttcatc aaagtcgcgg
     4681 agctggaaag tttttcccgc gcggcggatt tctttgcttt gccaaaagga agtgtttcgc
     4741 gccagataca ggcactggaa catcaacttg gcacccagct tctccagcgc accacgcgac
     4801 gggtcaaact cacgccagaa ggcatgacct attatcaacg agcaaaagat gtgttgagta
     4861 atctcagcga actggacggt ctgtttcaac aggatgccac cagtatcagc ggtaaattac
     4921 gcatcgacat cccgccagga atcgcgaaaa gcctgttact gccgcgcctg tcggaatttc
     4981 tctatctgca tccgggaatt gagctggaac tgagtagcca tgaccgtccg gtagatattc
     5041 ttcatgatgg ttttgattgc gtgatacgca ctggcgcgtt accggaagat ggcgttatcg
     5101 cccgtcccct cggcaaactg accatggtca actgtgccag tccgcactat ctgacgcgct
     5161 ttggttatcc gcaaagcccc gatgatctga cttcacacgc aatagtgcgt tatacaccgc
     5221 acctgggtgt acatccgtta ggttttgagg ttgccagcgt taatggcgtc cagtggttta
     5281 agtctggcgg catgttgacg gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg
     5341 gtctggggat tattcagatc ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc
     5401 ttattgaagt gttgccaggc taccgtgccg agccgctctc cctttcgctg gtttacccgc
     5461 agcgtcggga gctttcccgg cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga
     5521 aagagcacct ggactgaccg actatacttt ttaagaatga ccacgacaga aggacaaaag
     5581 agcggatgac gcaggaaaac gagatcaaac gtcccaccca ggatctggag cacgagccga
     5641 ttaagcagtt ggataatagc gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca
     5701 ccaccactgc cgaaaaagtc caacgccaac cggttattgc gcacctgatt cgcgcgacag
     5761 aacgctttaa cgatcggctg ggtaaccagt ttggtgcggc tatcacctat ttctcatttt
     5821 tatcgatgat cccgattttg atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc
     5881 atccgatgtt gctacaggat atcttcgaca aaattctgca aaacatcagc gatccgacgc
     5941 tagccgccac gttgaaaaac accatcaaca ccgccgttca gcagcgtacg actgtagggc
     6001 ttgtcggcct ggcggtggcg ctttattccg gcatcaactg gatgggtaac ctgcgtgaag
     6061 cgattcgtgc ccagtcgcgc gatgtctggg aacgctcgcc gcaagatcag gagaagttct
     6121 gggtaaaata tctgcgtgat tttatttcgc tgattggttt gttgattgcg ctgattgtga
     6181 cgctttcgat cacctcggtt gccggttcgg cgcagcaaat gattattagc gccctgcacc
     6241 tgaacagcat tgagtggctg aaaccgacgt ggcggttgat tggcctggcg atttccatct
     6301 tcgccaacta tctgcttttc ttctggatct tctggcggtt gccgcgccac cgtccgcgca
     6361 aaaaagccct gattcgcgga acatttctgg ctgctattgg ttttgaagtg attaaaatcg
     6421 tgatgaccta caccttgcca tcgctgatga aatccccctc tggcgcagca tttggttccg
     6481 tgctggggct gatggcattt ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt
     6541 ggattgccac cgccgaatat aaagacgacc cgcgaatgcc agggaaaacg cagccttaaa
     6601 ataggccgga tgcggcgtaa acgccttatc cggcatacga ttttattcgg cctggctccc
     6661 cgtaggccgg ataagatgcg ccagcatcgc atccggctat aatgcgcaca taacctcttg
     6721 aaactcttcc ccagagcctc ttcagccatc tatttgggag caaacaattt cattccaact
     6781 cataacccca gcatataaat ccagttggta acttttattt aacctgaaac cagttttatc
     6841 cactatttat aaaattatgt gaagcatttc atagaagaaa aatcactggc ctaaacatta
     6901 tccccttttt gcctggtttt tgaccatttc cgcgatttgt tacacattga aatatcactt
     6961 ttgctgtgcg taatatggct attcgttagc caaaaaataa gaaaagatta tgcaagcaac
     7021 agccacaaca ctcgaccacg agcaagaata cacgccgatc aactcgcgta ataaagtcct
     7081 tgtcgcctct ctcattggca cggccattga gttcttcgac ttttacattt acgccactgc
     7141 ggccgttatt gtgtttccgc atatcttctt cccgcagggc gatccaacgg cagcaacgct
     7201 acagtcgctc gccaccttcg ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt
     7261 tggtcatttt ggcgatcgcg ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat
     7321 ggggatttcg accgtggtga ttggtctgct gccgggctat gccacgattg gtattttcgc
     7381 cccactgctg ctggcgctgg ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg
     7441 cggcgcggcg ctgctggcga ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc
     7501 cttcccacag ctgggcgcac cgattggctt cttctttgcc aacggcactt tcttgctgct
     7561 ttcctggcta ctgaccgacg agcagtttat gagctggggc tggcgtgtgc catttatctt
     7621 ctcggcggtg ctggtcatta tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt
     7681 gtttgagaaa gtcgctaaag cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac
     7741 caaacatgtt cgcgtaaccg tactgggtac gttcattatg ctggcaacct atacgctgtt
     7801 ttacatcatg acggtctact ctatgacctt cagtaccgcc gccgcgccag ttgggcttgg
     7861 cctgccgcgt aacgaagtgt tgtggatgct gatgatggca gttattggtt ttggcgtgat
     7921 ggtgccagtc gccggattac tggctgatgc ctttggtcgc cgtaaaagca tggtaatcat
     7981 caccacgctg atcatcctgt tcgcgctgtt cgcctttaac ccgctgctcg gttctggcaa
     8041 cccgattctg gtttttgcct tcctgctgct ggggttaagt ctgatgggac tgaccttcgg
     8101 gccgatgggc gcgctgttac cagagctgtt cccgacagaa gtgcgttaca ccggagcatc
     8161 gttctcttac aacgtagcgt cgattctcgg ggcttccgtt gcaccatata tcgcagcctg
     8221 gttgcaggct aactacgggt taggtgcggt ggggttatat ctggcggcga tggctggctt
     8281 gacgttaatc gccctgctgc tgacccatga gacgcgacat cagtcgttgt aagtatcgtg
     8341 tgggcagcca ccctcacccc agccctctcc ctgaaaggga gagggagaaa agcggagcga
     8401 gatgttatat cgcgaacata acagccctcc cccagaaagt gaacggaaaa gcggtactcg
     8461 ttgtttcaac acgcacaaaa cagtcccctc gcccctttgg ggagagggtt agggtgaggg
     8521 gaacacgctg ctcccgaact caccagcaaa cacaatccaa cctcatcact tcttcatctg
     8581 cgacaatatc gtccgacact gattagcctc cccttcggaa ggtgagatca gtgccagtaa
     8641 cgccgccgcc ggtgttacca gcgttgccag tgccgcagca acagcaccac gggcaatcaa
     8701 cggcccggct ttcaccccag cctgcggatt tttaaacgtc ccacgcacat acagcggcga
     8761 acgcagtgtg ataatccgaa tccctttact ctccggatca atagtcaaat ccagctgttc
     8821 cgaagcaaaa cttgccgtgc cggtaacatt aatcaacgcg ttctcagtat cgaaagcaaa
     8881 aatctgcggg cgcgccacgc cgttggcaat attcagattc gccgccgcgc agttcacccg
     8941 cacctcatcg tcaccaaata tcgcaccgac aatgtagttg ccgacattca gcccaacaat
     9001 ctccatcagg ttgcggctca ccagcccgtc attcatcaac agtttcaggt tgccgttact
     9061 gttgcctaaa agtgctgcca ccgagttacc gctaccgcgt agttccgcgt caccgttcat
     9121 ttcccccagc gtcttctgca tcagttccac atcgggcatc agttctttca gtttcagtcg
     9181 acgagcctga atatctgccc gcccctgcat cggcttttta tcgccttcca gatgaatatt
     9241 cgccgcaatg ctgccgcccg ccatgccaaa tttcagcggt tgcaggcgca ggtcagcatt
     9301 tttgaggatg atatgagtag aaagatcgct aatcggcagg ctactgccat gctcaatgcg
     9361 ccgccctttg aagcgaacat cggcatccat aacgtcccat ttatcggttt cgaagcggtc
     9421 ataaggcagc actttgcccg caggctgaac gcttttttcg cccttcttct gttcagaccg
     9481 tttcgacttt tctgcccctt tcccggagtc aacgccaatt aacggtccca ggtccgccag
     9541 ccgcaattgc cgcgactcga catcaccttc cagttttggt cgtggttttc cggtggtgta
     9601 gaccagagaa ccgtggatat cgctatcgcc aattcgccca ttaaaaccgc gataatcaaa
     9661 gaccgacgat ttttcagtgt cgattttcgc taccagccga ccatccgttt caaacggcgg
     9721 ggtatcgggc agcagaacgc ccgtcagttc atagagatca cccagtgaat cgccagaaaa
     9781 tttaagccgt aaatcgacac cgcccatctt cattgggtca ttcacgacgc catcaaaagc
     9841 aacacgggtg ttaccagagc ggaaatcagc ctgtaccgga aacggcgtcc cttcgccacg
     9901 cagcgccagc ataccgccta tttttcccgt acccgtgagc ggttcaccgt tataacgtcc
     9961 ctgcgccttc aggccaaaaa cgtaatcgcc caccttttct ttatccgctt tacctttcga
    10021 tccagtaact tcgctgaacg gcagcggctt gcctaacgga tcaacaaaaa tctccagatc
    10081 cgctttgctt actttgtcat caatagcgat ccgcccttga tcgaaaagaa tattatccag
    10141 ccgaaacgac catgccgacg gctttgcatt cgcgtctttg ttatcatcgt tggcgagatt
    10201 aaacgtccag ttattgttct tttcggagag gcgaatcagg cgcgcgtcgg gcttttcgag
    10261 cttgatccac ggcagccaga ccgttttggt cagcagcgcc agcggggcca gcgttgcctc
    10321 tacgcgtggc aaatgcacca tcgtgacttc gggaatatcc ggtggattgc caagaatgat
    10381 gtcttccgcg tgtacatggg gccacggcac ccagctgcgc cagccagttt cttgtttttg
    10441 ccgctcccac accacgccca gatcgccacg gatagcgaac ggacgattca actccgcaga
    10501 gactttctgg ttgatggtcg gtttgagtcg attccagtca aaagttgcaa tcaaaatgat
    10561 cgccacgaca atcaacaaca agaaagcccc tgaaatcgca gcggttattt tgcctgcctt
    10621 gctcatcgtt cgcttcctca tattcttcct gtgccagtcc taaagatagt ccagccaggc
    10681 ggaaaatgag gcagcttata gcgccagaac cgccgtattc agcgtttcta tcggtgccgg
    10741 gcgtgaaaga aaccagcctt gtgcggcgaa tgcgggcgag ttctgaacat cacgccactc
    10801 ttccggtgtt tctacgccct cgacaatcac cccgcgacaa tagcgattca ttagatgtaa
    10861 aagctgagaa aagagtgtgc ggccttccgg cgactgacgc agcatcacga acagctctcg
    10921 cgcgattttg atgtagtcat aacgcacttc acttagcgca gagaaatttg ccatcccggt
    10981 gccaaaatca tccagccaca gcgggccaaa ttcacacatc gaggcaaagg ttgaatcttt
    11041 cggcaggcgg atgtgttcca ccagttcgaa acgcagccag ggaagacgct caatctggcg
    11101 caggattttt ggttgctgac gcagggcgat gagcgtaggg ccatcaatat tgaccgatgc
    11161 cagcaggccg tgctctataa agaagtcggc tttttgcgcc aacaaatcaa tctgctcttt
    11221 cacaacctcc atccgatggc tgacggtgat ttcagtaaaa tagcgatccg gcggcaggcg
    11281 ttgcgaaggg ttcaagggat gcgtgaccac cgttaatagc tccacggcca ttaaccgccc
    11341 gcatgtttga tagatcggct gccaggtgta agcacgctca cactgcaacc aaaaacgccg
    11401 ttcctgcaag ctctcgatgc ttgcttcagg gttgcttatt cgctggataa cctgccttat
    11461 catcaaagat gtcctgtgtt taaaagtgat gctgcccgga ctcgacaaag attatcggcg
    11521 cgtaagggca gaactttacc gcctggccgg ggtaaatttc ctcactccac aacggaatct
    11581 ttcaggaata cgttctggct caaaaaaata atggaacacc gttttaatat ggttgaccag
    11641 caaaccacca cagcgcaaac taacgctaat tttttacaga tcaggttcac gactatgtcc
    11701 aaaaagattg ccgtgattgg cgaatgcatg attgagcttt ccgagaaagg cgcggacgtt
    11761 aagcgcggtt tcggcggcga taccctgaac acttccgtct atatcgcccg tcaggttgat
    11821 cctgcggcat taaccgttca ttacgtaacg gcgctgggaa cggacagttt tagccagcag
    11881 atgctggacg cctggcgcgg cgagaacgtt gacacttccc tgacccaacg gatggaaaac
    11941 cgactgccgg gcctttacta cattgaaacc gacagcaccg gcgaacgtac gttctactac
    12001 tggcgtaacg aagccgccgc caaattctgg ctggagagtg agcagtctgc ggcgatttgc
    12061 gaagagctgg cgaatttcga ttatctctac ctgagcggga ttagccttgc gatcttaagc
    12121 ccgaccagcc gcgaaaagct gctttccctg ctgcgcgaat gccgcgccaa cggcggaaaa
    12181 gtgattttcg acaataacta tcgtccgcgc ctgtgggcca gcaaagaaga gacacagcag
    12241 gtgtaccaac aaatgctgga atgcacggat atcgccttcc tgacgctgga cgatgaagac
    12301 gcgctgtggg gtcaacaacc ggtggaagac gtcattgcgc gcacccataa cgcgggcgtg
    12361 aaagaagtgg tggtgaaacg cggggcggat tcttgcctgg tgtccattgc tggcgaaggg
    12421 ttagtggatg ttccggcggt gaaactgccg aaagaaaaag tgatcgatac caccgcagct
    12481 ggcgactctt tcagtgccgg ttatctggcg gtacgtttga cgggcggcag tgcggaaaac
    12541 gcagcgaaac gcgggcacct gaccgcaagt accgttattc agtatcgcgg cgcgattatc
    12601 ccgcgtgagg cgatgccagc gtaaaagcag catatgcaaa aggctggata aagtgttacc
    12661 cctctatcca gctcatccat aaatacctga tggttgtgct aactcatcac catcaggcat
    12721 tactgtgcag gtggaatatc cgtcacttca ggatgtacgt catccgtggc gacagaggtg
    12781 gtcgcagcgg cggtagatgg ggccatgatt tgatcccagg ccgcctgcaa cgctttcatg
    12841 ttaaattccg gctcgccttt cggctgcagc agtattaacg ccatatcatt cgacaactgc
    12901 tgacgcagat cctgatttaa catctccacc gtcaggctgt taaggaaatc ctgccgcagt
    12961 ttctgatact gctccggcgc gatatcgacg acctgatttt gcaacgaacg catccgctga
    13021 cccatcagaa tatcggtatc agcccgcgca taggcggcaa acagtttctg cagctccagt
    13081 ttcttttgcg ccactaacgc attgaactct tcttccggca gacctttatc gcgaaccttc
    13141 gccagttcac gcgccaccag attaaggttg ctgttcagct tgtcgtttgg tgattcgatg
    13201 ttgatggcac actgcgcacg cagatacagc acacggcagt caaatccaag accgatgtct
    13261 ttgctgttac tggcgcttaa cgcttgctga acatgccaga acagcgcctc gcgggccagg
    13321 tccgcacgcc agtagcgcag cagtgcggct gattcacgaa tcggctgcca cggcgtatcc
    13381 cacatgatag ataaccggtc ctgacgcacc gcgtcagtca taatactcac cgcttccgca
    13441 cgcagcggag aaagcgtcgg caccggagct ggcgtttcac gtttgccttt cagttcgcca
    13501 aacgttttgt ttatttggtc gacaaccgag cgcgcatcca cgtttcccac caccagtagc
    13561 gtcattgcat ccggggtgta ccatttctga tagaaatctt taattttttc cgcttcaacg
    13621 ggttgtttca gcggatcggc aggatcgtga cctaacaagg ttgatccttt cagacgataa
    13681 cgccaccagc cctctttagt atcggcaggc caggttgcca ccatgtcctg actttgcagc
    13741 gcgtggttga tggtttctgg cgtgatagtc agtttgccag tggcatttgc caggtaagag
    13801 agcgcctctt tcagcaaatc gttacggtta ttgggcaaac tcagattaaa cagcgtagtg
    13861 tcatacgaga caattaccgg cggcatcggg cgtttagggt cgatcccctg ctgccacaat
    13921 gaacgcgcct gtgctgcgtc aaggccaccg ctttgcgtta gcgcaatacg agggatggcg
    13981 tgactgtaac cgctctgttg tgtactttcg gcgagcgaac cggtattaac cagcaggcga
    14041 atttcaacac gatcgctggg acgctggggg gtggtcagca cttgccactg taaaccgttg
    14101 gaaagcgtcc cctgttgcca tgctggatca ggctggagcg catctgcctg cacatagcca
    14161 gcagtggcca tcatcagcaa accgcccgct aaaagtcgaa tttttgtgcc ctgcatgtga
    14221 acccctgatc aactatcctg gtaataaaaa aactgcccgt tgtcggtcag tctaaatgac
    14281 gttaaaaaca cttcgtgtta gaccgcaaga acatgaaaat gtcacggaag aagtgaaata
    14341 aacctgaact cacccaggtg gtaagttcag gcacaggggt caattatgcg caaacacccg
    14401 cactcgggga agggagtgcg ggcataagtg atgagattaa gaggataatt cgtgcgtttt
    14461 gccatccggc gcacgattat tcagcacatc gtccagtttt ttgtggtcca gttctttcac
    14521 ccacttagca acgacaatgg tcgctacgcc gttaccgacc aggttagtca gcgcacgagc
    14581 ttctgacata aagcggtcga taccgaggat cagcgccaga cccgctaccg gcaaatggcc
    14641 caccgcagag agcgtcgccg ccagcacgat aaagccacta cccgttaccc ctgccgcccc
    14701 tttagaagaa agcagcaaca cgattaacag cgtgatttgg tggacgatat ccatctgact
    14761 gttagtggcc tgggcgataa acaccgccgc cattgtcagg tatatcgatg tgccatcaag
    14821 gttaaacgag tagcctgtcg ggatgaccag ccccaccacc gatttgcggc agccgagttt
    14881 ctccatcttg tcgagcatac gcggcagcgc cgactcggaa gatgaagtcc ccagtacaat
    14941 cagcagttct tcacggatgt agcggataaa tttgaagata ctgaaaccag tcgctttagc
    15001 gattgaaccc aataccagca ccacaaacag gatacaggta atgtagaaac agataatcag
    15061 ctgccccagt tgcaccagtg tgccgacgcc gtatttaccg atggtaaacg ccattgcccc
    15121 gaacgcacca ataggcgcca gacgcatgat catattgatg atgccgaaga tgacctgcga
    15181 gaaactttcg atgacgttaa aaatcagttg gcctttgctg cccagacggt ggagcgcaaa
    15241 accaaacagt acggcaaaca gcagcacctg cagaatgtta ccgctggcaa atgcgccaat
    15301 gacgctcgcc gggatgacat ccataatgaa ggcgacaatg ccctggtctt tcgcctgatc
    15361 ggcgtaaacc gctaccgctt tcgcatcaag cgttgccgga tcgacgttca ttccggcacc
    15421 aggctgcacg acgttaacga tgataagacc aataatcagc gcgatggtac tgacaatttc
    15481 aaagtaaagc agtgcgactg cgccggtacg accgaccgcc ttcatgcttt ccatgcccgc
    15541 aatgcccgtt acgacggtac aaaagatgac aggagcgatg atcatcttaa tgagcttaac
    15601 gaagccgtcg ccaagcggtt tcatttgctc gcctatttca ggatagaaat ggccaaggag
    15661 aataccaatg gctatcgctg tcaggacctg aaagtaaagg cttttaaaca gagaggtttt
    15721 catagggtgt cctttagtaa aaccacaggt cttgtaaggt tatggggtac ctgcggcctt
    15781 aaaataacac ccagacaaca tcacagaaat gtacctggat cataattgaa acaaaaaggt
    15841 taaaaagttt gagctggctc gcacaaacca gcacttttta aagttttgta atcagtttgg
    15901 ggtagctact tttcttccag gtaactctct tcgaagattt caatagggag tgggcgagca
    15961 aaaaggaagc cctgggcaat accaacgccc gcttttgcca gccagtcgcg ttgtgcttca
    16021 gtctccacgc cttcggcaat catttgtaag ttcaggctct gcgccagcat gataattgca
    16081 gcaatcatgc tgctatcttc cggcaagcct tcaacaaaca ttttgtcgat tttcagtacg
    16141 tcgattggca acgatttcat atgctgcagc tgacgcagcc ctgcgtagcc catgccgaaa
    16201 tcatccagcg ccacccgaac tccggcattg cgcagcggac ggaggattgc caccgcagca
    16261 tgagggtcgt caatacgtcg gctttctgtc acttccagaa tcagtgttcc cggctgaatg
    16321 cgatagcggg ttaacagttc cagcatatcc gccaccatat ttgggtgcat cagttgcagc
    16381 gcagagaggt ttaccgacaa gggcagcata atgccgcgct cttgccaggc tgcaagcaat
    16441 cgacaggact cttccagcac ccagtgaccg acggtaacca tcagcccaca gcactcaatg
    16501 cgatcgatta agccatccgg caggtcccaa ctgccatccg gttgctggat acgcagtaac
    16561 acttccgcac tgaccagttt accgctggtc atctcgacct gtggctgtaa ccaaattgca
    16621 aactgatgat tttccagtgc attaaggata tcgctctctt ccgtcaaccg cttctgggcg
    16681 gcttccatct gctgcggatc aaagaactga atctgattct tgcctttatg gcgagcggta
    16741 aatgccgcag aaatagcgcg actgtaaagc tgttcggcgg tgagatcgcc gtagaacatc
    16801 gccacgccaa tgctacagtg cggacggagt tgaatacgtt caatcggcag gcgctcgctc
    16861 atgatagtga gcacttgctg acctaaggtg attgcgtgcc acggttcctg tacaccgttg
    16921 gcaatgacag caaagtcata accgctaatc tgcgcgagga tcatacgtgg cgacagtacc
    16981 gatttgagtt tttccaccag cgtcagcagc agaatttctc gttgcgcctc tttcagcacg
    17041 cccgcagtat cacgcagggt ttcacaggtg atgatcatca gcgcggtggt ttgtttacgc
    17101 gcgacaacct gctccagcat ctccatcagc aaggctttgt tcggcaaatc cgacaccggg
    17161 aagcgcatca cattttcgtt ctgttcttca taatggcgct gcagcaattg ctggttgagg
    17221 ttgtaactgc gcaccaacat accgatttca tcgtcctgat gcagacgcgg taatgccagt
    17281 tggtgaccaa caagctcctg ggctgggatg gcgttaagtt cgcgagcaat attgcgtaac
    17341 ggatgcaaaa tcaggcggtt aatgcaccag ctgatggcga cggtcaggat aagcgacaaa
    17401 agtaagtaaa tggtcactaa cgttgagagg gtgctcatca cgaacttata catacggaag
    17461 gaatccgcct gtagtaccag ataggcaatt ggctgcgggt ttgccggacg ttcgagcgag
    17521 taaacgccca gcgagatttg aaccggtagc tcaaacaggc gagtaaccat taccggcacc
    17581 gggcgctctg gaataaaact tttacgcagc gcctggaact ggttaggcag cactacatcg
    17641 gcacggctga ccacgcctgc cggtttaatg ctggcaagaa tagcttccgc ttcgggaata
    17701 tcgcctttca aaatagctga agataagggt tcgcggacag agcgagcaat gctttccagt
    17761 tgcgtagccg tgttatagcg attctgttgg accagatgga acagcaaaac ggtgcaaaaa
    17821 ataaaaacga acaccaggac aacggctgcc accattgcca tctgcttgat tgttaacgag
    17881 cgacttacgc gcaaattgtc tccccccgga aatcccagat tgccgcccga gtatacccga
    17941 tcgcagcggc attaagagag gcgctatctg aaaacttacc agtcggcgta aggtatcagc
    18001 ggctgcggcg gtaaatccat gtcaccctgc catccggcgg cggaataacg tacgtagagc
    18061 aggaaatggc tgggtgcgta atctttcgcc tgctggatat caattgccgt gccaacaaac
    18121 cagttggaag taacacgtcg ttcaagtaat gcccgcgccg tgtagccgaa gccctgactg
    18181 ctgccgccat cgttggattg ccgcgcagct tcttcctgcc agtcggtcgg gatcagattc
    18241 atcagcggat aacgcggcat ggttttggtg cgtgaatgcg accacgagcc agacgccccc
    18301 agctcccacg accagttttc cgtgcgctcc cgccacatca ccggtatggc aaacgacagg
    18361 tattcctgcg gactgtagta accgccctga ccgagtgagt agccactcag atctttgtcg
    18421 taatgccaga tcatgttgtt caggccgatt gtgacgcggc gattgttctg gttaatgacc
    18481 ttatagtaat agcccgtcat ccagcgcacg cgccagttat cttcgacatt tttaccggtt
    18541 aactggtcgc cactaagcga tgcccagacg ccgtttgctt cacctttatc gtagctcaga
    18601 cttagcccca caccgtcggc acgtacgcca ccccattttt tcccggtatt gctcggggag
    18661 tctttttgcc caccaaaggc cagcaaagaa ctggagatgg gccgacggtg ggcgttaacg
    18721 gtgtaaccca gcggcccgat atcatcgctg taactgatgc cgccgaccac atccaccacg
    18781 ttgaagccca tcggcgtggt accgatatcc cagctccaga cgtcatttcg ccagccgacc
    18841 gccacgctgg caccggaatc cgactggctg cggttgccgc tacagtcctg taatgtacag
    18901 gtgccccagt tgtcatccca tttgccatcg gcattagtgg agaaactgcc gacgttcata
    18961 ttgacgaaat cactgcgaaa gaacatccgc ccgtcagaat acggcgcatc cacctgcaac
    19021 atggtagtgt gcgctttcag atcggagtaa ccaccggtgc cgctcgaacc ccagtaatcg
    19081 tgctcaaggg tgacgttaag atcctgctgg cgatagaggt ccgccgcatc gctgcgcacg
    19141 ccgcgtttca gccagtcatc tttctcgtcg ttacgggtca gtcgggtgaa tgtgtcgtta
    19201 tcctgcggac gcgtcgtagt cacaccggat gcgaccatgg cgtctttgta ggtttccagc
    19261 gcctgctttg gatcgcccgc ctgcgcttca aacttcgcac cgtcgcgcag caccatcgcg
    19321 ctttccatcg acggtggctg agattttgcc tgcgggatca acttattaaa cgtctgctgc
    19381 gccgctgcgg tatctccaag ctgcgcctgc gccagcgcca cgcgccgctg tgtgttcagc
    19441 gaggcgttat cggtagcggg cagtttcgcc agctggctac gtgccgccgc tgtgtcaccg
    19501 gcagcaatat ccacttccgt cagccccaga atggcatcgg cgttagttgg ctcccgcgtc
    19561 aggacattct gatatgcagc gcgggcggcg gtgtaatcac gtcgctgctg cgcccagtca
    19621 gccagcgtga ggtcaatacg cgtggaaggt ggttgctgac gcagcatcgc ttccgcttca
    19681 gcttctttgc cgctttctcg caggcgatta gcggtttcca gcacctgatc gctttgcagt
    19741 cgattaacca gctcctgaat attgctgttc cactgcgcgc gcggcaggct gttgatatgc
    19801 gccagcgccg ctctgtcctg gtcatgacca gagaggtaca gcccgtaagc gtaaacctgc
    19861 tccgggtcgt tcggcttctg ctgcgccaga ttgcgcatta acgtatcggc ctggctgcgt
    19921 tgtccggcct gccagagatc ctgcgaaagt cggtaagtaa tccatacgct gccggggtcc
    19981 agcgccagtc gttgccgctg aagtgctgcc gcctgcgccc atttgccctg gttttccagt
    20041 gcctctgcct gctgtgccag acggtcgttt tgcaggctgc gttcgatatc atcaatgcta
    20101 cgccgctgac tggcagagag cgaggcgata aacgcttcag ctttttctgg cgattgctgg
    20161 cggtaaatat ttgccagccc gcgcacggcg ttagtgttgc cgctgtccat acgcaaggtc
    20221 tgctgataat aacgttcggc ggcgggataa tcttttcgcg ccatcgccac atcgcccagc
    20281 cccagcactg cataactgtc ggtgttatcg acattacgcg cctgctggaa caggcgttct
    20341 gcccggtcag gattattggc tttcagcgca gcatcgccct gctggatcgc cagccagtag
    20401 cggtttactt tcagcagact gttccatttg tcgttgttgc tgctgtgcgg gtccagtgcg
    20461 agggcttttt ccagattcgc cactgcattg gcgcgatcgc ctttctgaga atacgcctgg
    20521 cccagcgccc ccagagcttc actgtctttc gggttcgccc gcaccgcctg ttgtagttcg
    20581 ggaatggctt tacccgccat accagagtcc accgccgcta aaccttgcgc acgagcgcgg
    20641 aaagcaggat cggccagctg tttttgctgt tctgccagtt gcgattgcgc agccgccacg
    20701 ctatcgccat cactaaagat cgagagatat tttttcagcg ccgacacact ggcatcactg
    20761 acgggcatgt ctttaatctg cccgtaccag attttagagg ccccttcgcg cccggcgttc
    20821 gattttgcca tctgttccag gacggcaaaa ccttcgtcac ggcgatcgct actaaacagc
    20881 aatagcgcca gattgttttg caggcccgta ttgcccggtg catccgcatt gatgcgtttt
    20941 aactgattaa tcgcttcgcc acggcgagcc ggaattttcg ccaccgtact ccagtactcg
    21001 acagcaatgt caccttccgg cggcgcaccg ttgaacagtt tgttgtaact cgccacagct
    21061 tcttctgcat gaccggtcgt cgcctgcaat cgtgcctgtt gcagtgcctg acgaccatcc
    21121 ggcgtggaaa gtagcatcgt agtccgcgac gatttatacg catttgaact cggcgctaac
    21181 tgcgacagcc gatcgagctg tttttgcgcg ccatcaatat cgccctgacg taacaaagaa
    21241 cggaaacggg cggcaacgac gtccgggtta ttcggatcaa taagttccag ccgatataac
    21301 gactgttgca ccagatcttc acgatgggtc gcttcgccta accgaacttg ctccagcaac
    21361 tgttgctgag cggttggtgc tgcctcgacc atcggcatga cggccagacc gagggaaagc
    21421 gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat aactcacctt
    21481 ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc agcacatagt
    21541 tgtaataggc atcgctgccg ggaaagttat cggccacgcg ctggcgctga acggcctgcg
    21601 catcgcggtt ttgtaaaaag ggcagcatgg cggcagaaaa accgaccggt cctttaccct
    21661 gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc tcagtgaatg
    21721 tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg cggatcgctg tcaggcatca
    21781 tgcctaccca catgtaaacg cggatagcgt cgtagctgct gatcaatgtt ttttcggctt
    21841 ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaaa cctttcgggg
    21901 cggtttccag caataaacgt tgattggttt cgcgcagcgt ggtccacggc gcgccaaagc
    21961 gggtgaaata ctgcgccagc gtcggcggca ggtagctggg gttaaaacgc cagctgttat
    22021 cctcagcaaa acccactttg cccggtaaca acatggaacc cagcccaggc accgtcacca
    22081 cttcctcccg cgcgatacgt tttagcaacg cgctgccgat gtcggtataa cgctgctctt
    22141 tccacaaacg ccccgcctcc agcaacgacc aggccatcca gacatcacca tcggaggccg
    22201 aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc caggcgggca
    22261 aacgttcttt taaagaaccc tgagcgagat tgttctgcgt ccagtcgaga atattatcga
    22321 aagctacacg gtcgttagcc gccagggcaa agaacatacc gtaactttgc ccttcggagg
    22381 tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg atgtaatcct
    22441 ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag gcagccaaca
    22501 gcagcatcgt cacgattcca ctacgcaaca cattcatctt caattactcg ttatccgggt
    22561 taagacgacg acgactaata attcgcagca gacgccacag tacccatgcc agcaatatca
    22621 cactgatagc cgccagcacc gccagcagaa tcggatggtt tgccagcgca taccacaagc
    22681 gctcgaacca cggcagatga cctacgtaat aaacgtcgcc aacacgtagg ctgttgatac
    22741 cggactcgcg gatcaccgcg accgaaccga acatggtggc gcgtttgccg ctatcgttca
    22801 ccgcatcgtt aagcatttca taaccgcgtg ggctatctgc cagcagcgca atcacgctgc
    22861 gctggtcgtt atacggcgac tggaagccaa tcaccgccgc catcgcaccg gaagaggtca
    22921 gcgttgactg ggtttctgcc gcgcgatcgc tctcgtccgg cacaatgccg gggaacgggg
    22981 tctggcgcat cggtgttttc acccagcttt cggtcgcctg caccaatagg tcgatctgct
    23041 tatcgtcttt cagtttgtcc gggataccac cgatgatcat gatgtcggca tctttgccct
    23101 gaatggtgct gccatcatcg gtcaccgtca gattaatcgc cgggaagccc gtctgtgcgc
    23161 cgataaaacc aacagtattc agcaacgttt ccatctgtgc ttcgttaggc gctttcggca
    23221 tcacggtgat ggtttgcgac agatccgcca tccggctgaa tgggaagccc gcgttagcaa
    23281 aggcgcgtag atccggcatc gggatgaagt ggtaatactt cgagaagtcg atggtggagt
    23341 cgtcaccaat caccacatga ttctgcaccg gctggaacgt aatacagtta tccaccgaac
    23401 cgcccggcat cgggttcata tactcaaagt cgaagcgcag ctggttggtc gcgcccagtt
    23461 tcagcgccgg aatagagaca tctgttttgc catccagcaa accttgtaat accggaatcc
    23521 gcagcagcag gcggttcgcc tcctgtttgc tgctcaggtt gaaggattgc aggaactggt
    23581 tattcaggct gatatccatc cgcgaactgt ctttcaccgg cggcatggtg tagcggtaat
    23641 taatatccat atcaatgccg gtactgcgca tcaggtagag atccggcggc aggtttagcg
    23701 aaacgttaat cgctgctggc tcaagaccgc tggattgtaa ctgttcttca taggttttca
    23761 gttcgccaaa tgtgaccgga cgatcggtac gtacccagtt cggcgcatcg tacggcttac
    23821 gcggtagcag cggtttcact tcattcacta ccacgctttc accacggaac agaatgttac
    23881 cctgagcgat acctttcgct gcctgcaaca ggtctttgtc gtcacgacca aacaccacca
    23941 gcagtttgac gtaaggattc tgcggatggt taatcatctc aatcaccggg gcttttaccg
    24001 ccggatgatc gcgcaggaag tccggccgtt tgtcgttggt tgcaaagaca atggcattac
    24061 gatccggcag ttggttatag agcaccggga agttctgccc acgccagcca gaacgcgaac
    24121 caaaccacga ggcgacaatg gcagaggctt gttgcagccc aacatccggc gcacccgcaa
    24181 agaccatcgg caaggtgttg gtgcggttat cacgcggatc aaagaatggc accgggaagt
    24241 gtgacaggtc attcttcaca ttcagggtct gataggtcag atccagtcca ctgctccgcc
    24301 caacatccag ccaaagcgtg gtgctggccg ggttttcgca cacgtcctga taatggccga
    24361 caaactccag ccgcacacgg ttgaagtcgg taataaacag tgggttaatg ggcatttgcg
    24421 ccagcgtttt tttacccaac tgttctttgg tcactggcag cacgcccatc agctcatcat
    24481 tgagataaac ctttaactgc gactggacag gcagtaacga tggcgatggc gtgtattcga
    24541 ggttgagcat cgccttcgtc accacttcat cgctgcgcat accaaactca atgctgccgt
    24601 tcgggttaat gccacgtagc accatgctgc ccggcggcgg cgcaatttgc gcaaaggtca
    24661 gcttcacatc acgcgaagga ccgttctgcg ccacgactgg cgcatcagcg ccctgcacgc
    24721 ccggcatcac ttgccccacc tgcggatttt gttccgtctg ggcggctaca gctggctcag
    24781 cattgatcag tggttgcgtt gctggcgtcg cctgcgtcat gaaagagggg aacgcactca
    24841 tccccatagc cactgcacaa atccagaata gttttctttt catcgcgtta tcatcattgt
    24901 tgagccaaag cctgatccga tggttgtgcc gtttcgctcc gctccgggcg gcgcggaata
    24961 aacgatacaa cccaggaaac cagagaagtc agcacacgga atatgccctt caccgaagaa
    25021 ggcgcaaact ccgccagatg gcggtagcca cggaagccga gcttcagaat atccagcaga
    25081 ctttccagcg gcttatcttc cgggtagctg tcctgccaga gcgcccatgt atccgcacgg
    25141 gcaaacgtac actgcacaaa atcgatatgt tgctgggtgg tgagcggcat taatttcagc
    25201 ccaacttcat tacccatcac gcgcgccacc tgggtcggga agacgtattc ctgctgaccg
    25261 cgtttaagca acagattcac tttctgccct tccagaatct gcgcctgacc gttgatcttg
    25321 atccccaaac caccgtcgga gaaatcctga acggtacacg agaagaggtg accatcttcg
    25381 cgggcaattg ccgcgggcat cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt
    25441 tctaccgata ctgcaactgc gccgccaaga acaatcaggt tgtagaatac ccacaccata
    25501 ctgacgacca cggtgagcat ctcggttggc gggccataga agtagcgcca gatgcctacc
    25561 gcaacgccca ccaggttgag caggacaagg aagatgtagg gccgcgagat cacccagtcg
    25621 acgtactctt cttccaccag tccacctttg gcggtgacgt taaatttgcc tttgtgcggg
    25681 ttaatcagcg ccaccagcgt cggtggtgcg atataccacg ccagcaccgt ttcgtagatt
    25741 tcactccaga aagagtggcg atatttgccc tggatcttgg agttggtcag gctggcatgg
    25801 atcatatgcg gcagcacgaa cagggcgatc atcaacgctg gcgcatagat gatgtaggca
    25861 tgaagcagca ggaacgccag cggcgcagtc aggaagatca gtcgtggaat gcccgacaag
    25921 aagtggaaca tggcgttgac gtagcacagc cgctgggcga atttcagccc tttaccggta
    25981 agcgggttat cgagacggaa gatttgcacc atcccgcgtg cccagcgaat acgctgaccg
    26041 atatgcgccg acagactttc ggtcgccagc cccgccgcct gcggaatacg catatatgca
    26101 gaggtgtagc cacgacggtg caggcgcaga gacgtgtgcg catcttcggt taccgtttcg
    26161 acagcaatac cgccaatttc atccaatggt ttgcgacgaa ttactgcaca ggaaccgcag
    26221 aagaaagtgg cgtcccacat atcgttgcca tcctgcacca gaccatagaa cagcgtgcct
    26281 tcgttcggcg ttttacgaaa acgccccagg ttgcgttcaa acgggtccgg cgagaagaag
    26341 tggtgcggcg tctgcatcat cgccagctgt ttctctttca ggaaccagcc catggtcatt
    26401 tgcaggaacg atcgcgttgg tacgtggtcg cagtcgaaaa tcgacacgaa ctcgcctttg
    26461 gcatatttca gcgcattgtt gatgttgccc gctttcgcat gttcatgagt ggtacgggcg
    26521 atatatttca cccccacgtt ttgcgcaaac tggcgaaact cttccctacc gccgtcatcg
    26581 aggatccaga tgttcagctt gtctttcggc cagtcgatac ccagcgaggc gtaaatagta
    26641 tttttcacca cgttgagatc ttcgttgtaa gtcgggacaa agatatccac cgacggccac
    26701 agcgacatat ctttcggcaa tggcaccggc tgacgattca gcggccatac tacctggaag
    26761 tagccgagca ccagcacaat ccacgcgtac gtttcggcga agagcagaat aagcccgcac
    26821 accaggctga ccggatcgtc ccagttcagc gtagaggtgt aacgccacca gatataacgg
    26881 caagaaacag tcagcgacag cacaatcaac attagcgctg agaagcgccc cggcatccgc
    26941 cgtacgatca gcgctacccc ccacagcagc atcaggaaga taaactgcgc cagcgggtta
    27001 aacggctgag taacgcagat taacgccaga atcagcgaga aggtgacgat gataccgagg
    27061 atcaaccgcc gcgccccggc actcaaatga ccgagctctt ttttctcatc aagatgctgt
    27121 gttttatggc taacgcgctc aggcagctcg ttcatccatt gatggtaacg tccacgaata
    27181 ttctgcagac ctgaaaatgc ccgcctgcgc ggtttcggcg tttctttgcg cgatgcaccg
    27241 atcagtaacc agcatgtttg aatgagataa cggaccgggt ccagcggacg cggacgcgag
    27301 gcgttgatat gcggatacag gtttttatgt tctgcgcgaa tacgctgcca gcgcgggtgc
    27361 tccagcggaa taaaaatcca ggccaggatc atccagaaac agccgagcgt cgcgctgaaa
    27421 gccgacgcac cgtgacgacg ataatcgcga taacgcccga taagccgcgc gttgaccggc
    27481 gggataagca accaccgggt caggatactc atgatgcact cccgactggc gttttcagcc
    27541 cggagtagtt caacaggcac cagttcgcca gcgtcagtat ctcttcagcc gccagcgcat
    27601 cactgcgata ttcacctact ggttgcttag ccgccaggca ttcagccatc gcttcatcac
    27661 gatgaatgag catcggcagt aatcggcgct ggctttgcaa ccaaagctgg taaatatcgt
    27721 cctgaacctg accgccaata cggaagtcat taatcaaaat atgtgcgcca tccggcagcg
    27781 cttgctgatg cagtcggata tggcagttgg catccacatt gacgattgcc agcgagtgat
    27841 cgcacaaact cagcagctgg tgggttatct gcgaggcatc acgcggtaag tcgattaaaa
    27901 tccactggta acgcccgctg gcttttagtt gctgtaagcc ggagcaaata tcgctcagcc
    27961 gggtttgcca gtgctgtgga ttttcttgtt cttcaatgga taactgacca aaaggcagca
    28021 aatcgagctg cgaggtgtag cgcaacccag cgtcacgcca gtcctggcca tccagcatcg
    28081 ctctggccca gccctgacgg tgggtaaaat caacattaaa tgacaggcgc aacaagttgt
    28141 ccgggcaggc atcgaccacc agaacatttt ctcccaacat ttgtaatgac caggctaatg
    28201 cggcggtgat ggttgttgtc cccacgcctc cccgcacccc ctgcaatccc agtacggcca
    28261 ttcaccaatc ccctactttt gttgcgcaaa ctctgccagc aacggccagc gttttaatgc
    28321 cgcggccaac tgttcgcgtt gggaaatatc ggcataatca atatcaggca gtgaaaatgc
    28381 ctgctttaac gccacaatat cattctggaa gatatagcct atcgcgggat caggcagagt
    28441 atctggttca ttgttattca ttttgcttga tccctatgaa ataaccgtac cgttcatgag
    28501 acaattctaa ccgtgagcat ttcgccggtt tattgtttac ggcgagacgg gaatttgctc
    28561 actttttgac tttccgttac gcaacagaaa aagttaaaaa cgaaaagact aaaatcacac
    28621 gtcttgtgac cttctaaatt gattcacatg ctaaatctga taagttttaa tttcaatggt
    28681 aggtttattt cttagctttc gctagtaaac tgataatcag acaaaatggt gacatgaggg
    28741 acattgtgga ccctgtattc tctatcggta tctcatcatt atgggatgag ctgcgacata
    28801 tgccagcagg cggcgtctgg tggtttaacg tcgatcgcca tgaagatgct atcagtctgg
    28861 cgaatcaaac aattgcatcc caggctgaaa ccgcacacgt cgcggtcatt agcatggaca
    28921 gcgatccggc gaaaatcttt caattagatg attctcaagg gccggaaaaa ataaaattat
    28981 tttcaatgct aaatcatgaa aaaggtctat actatttgac ccgtgatttg cagtgttcta
    29041 ttgatcccca taattacctt tttattcttg tttgcgcaaa taacgcatgg caaaacattc
    29101 ctgccgagcg gcttcgctca tggttggata aaatgaataa atggagcagg ttaaaccatt
    29161 gttcgctttt ggtaattaat cccggaaata ataacgataa acaattttca ttgttgcttg
    29221 aggaataccg ttcacttttt ggtcttgcca gtttgcgttt tcagggtgac caacatttgc
    29281 tggatattgc cttctggtgc aacgaaaaag gggtcagcgc ccgtcagcag cttagcgttc
    29341 agcaacaaaa tggtatctgg acattagttc aaagcgaaga ggcggagatc caaccacgca
    29401 gcgacgaaaa acgcattctg agtaatgttg ctgtactgga aggtgcgccg ccgctatcgg
    29461 aacactggca actgttcaac aataacgaag tcctgttcaa tgaagcccgt accgctcagg
    29521 cggcgacggt ggtcttttct ttacagcaaa atgcgcaaat cgagccactg gcccgcagca
    29581 ttcataccct gcgtcgccag cgcggtagtg cgatgaaaat cctcgtgcgg gaaaataccg
    29641 ctagcctgcg cgccaccgat gaacgtttgt tattggcctg cggtgcaaat atggttattc
    29701 cgtggaatgc gccactctcc cgttgtctga cgatgatcga aagcgtgcaa gggcagaagt
    29761 ttagtcgcta tgtgccggaa gatatcacta ccttgctgtc aatgacccag ccgctcaaac
    29821 tgcgtggttt ccagaagtgg gatgtgttct gtaatgccgt caacaacatg atgaataacc
    29881 ctctcttgcc tgcccacggt aaaggcgttc tggttgccct acgtccggta ccgggtatcc
    29941 gcgttgaaca agccctgacg ctgtgtcgcc ctaaccgtac cggcgatatc atgaccattg
    30001 gcggtaatcg gctggtgctg tttctctcat tctgtcggat taacgatctg gataccgcgt
    30061 tgaatcatat tttcccattg cctactggcg acattttctc aaaccgtatg gtctggtttg
    30121 aagatgatca aatcagtgcc gagctggtgc agatgcgctt gcttgcccca gaacaatggg
    30181 gcatgccgct gcctttaacg caaagttcta aaccggtcat caatgccgag cacgatggtc
    30241 gccactggcg acgaatacca gaacccatgc gactgttaga tgatgctgtg gagcgctcat
    30301 catgatgacc atcagcgata tcattgaaat tattgtcgtt tgcgcactga tatttttccc
    30361 gctgggctat ctggcgcggc actctttgcg acgcattcgc gacaccttac gtttgttctt
    30421 tgctaaacct cgttatgtta aaccggccgg gacgttacgc cgcacggaaa aagccagggc
    30481 aaccaaaaaa tgactcaatt tacacaaaat accgccatgc cttcttccct ctggcaatac
    30541 tggcgcggcc tttccggctg gaacttctat tttctggtta agttcggcct gttgtgggcg
    30601 ggatatctta acttccatcc gctcctcaat ttggtgtttg ccgcgtttct gctgatgccc
    30661 attccgcgct acagcctgca tcgcttgcgc cactggattg ccctgccgat cggctttgct
    30721 ttgttctggc atgacacctg gttgcctggc ccggaaagca taatgagcca gggttcgcag
    30781 gtggcggggt tcagtaccga ttatttaatc gaccttgtca cacgctttat taactggcag
    30841 atgattgggg ccatttttgt tttattagtg gcctggttat tcctgtcaca atggattcgc
    30901 attaccgttt ttgtggttgc catactgcta tggctgaacg tacttaccct ggcgggacca
    30961 agtttctcct tgtggccagc cggacaaccg acgaccactg taacaacgac gggtggtaac
    31021 gcagcggcaa ccgttgcggc gacgggtggc gcaccggtag tgggtgatat gcccgcacaa
    31081 actgcaccgc caacaacggc gaaccttaac gcctggctga ataatttcta taacgcggag
    31141 gcgaaacgta aatcgacctt cccgtcttcg ctgcccgctg atgctcagcc atttgaacta
    31201 ctggtgatta acatctgttc gctttcctgg tcggatatag aagccgccgg gttgatgtcg
    31261 catccactgt ggtcgcattt cgatattgag ttcaagaact ttaactccgc cacctcctac
    31321 agtggcccgg cggcgatccg tttactgcgc gccagctgcg ggcagacttc gcacactaat
    31381 ctgtatcaac cggcaaataa cgactgctat ctgtttgata acctttcgaa actgggcttt
    31441 acccagcacc tgatgatggg gcataacggc cagttcggcg gttttttgaa agaagttcgc
    31501 gaaaatggcg gcatgcagac tgaattgatg gatcaaacaa atctgccggt tattttgctg
    31561 ggctttgatg gttcgccggt ttatgacgat accgccgtgc ttaaccgctg gctggacgtt
    31621 accgaaaaag ataaaaatag ccgtagtgcc acgttctaca acacgcttcc actgcatgac
    31681 ggcaaccatt atccgggggt cagcaaaaca gcggattaca aagcgcgggc gcagaaattc
    31741 tttgatgaac tggacgcctt ctttactgaa ctggagaaat cgggtcgtaa agtgatggtg
    31801 gtcgtggtgc cggaacacgg cggcgcgctg aagggcgaca gaatgcaggt atctggccta
    31861 cgtgatatcc ctagcccgtc tatcacagac gtccccgttg gggtgaaatt cttcggcatg
    31921 aaggcaccac atcagggggc accgattgtc attgaccaac cgagcagctt cctggctatc
    31981 tccgatctgg tggttcgcgt tcttgatggc aagattttca ccgaagacaa tgttgactgg
    32041 aaaaaactca ccagtgggtt gccacaaaca gcaccggtct ccgagaactc aaatgcagta
    32101 gttattcaat accaggataa accgtacgtt cgcctgaacg gcggcgactg ggtgccttac
    32161 ccgcagtaag cacgaaaaag gccgcagagt ttaacctctg cggcctgatc cgggcgcaaa
    32221 ttgccattac ggcagcctga cgcccgcatg acacgttact tccgcttatt cagccagttc
    32281 acgatcatac tggcaagaat gccagcaatg accggagccg ctaaatcatg ccagaaggcc
    32341 atgcccagct ctgcgagcgt catatagccg cctgtgttgt aatgacaacg tttcgcggct
    32401 attcttgagt tgtttgggtt caagattagc ccccgttatg ttgtcaggtg catacctgca
    32461 acgtgcgggg gttttctctc tccagcaacc aatgccacca gggataaagc ccccgcaaca
    32521 ttgcgcctta ccggaacctt tccggcttgc cgctgattct acgactgttt ttctgttccg
    32581 aaatccatca cttactcact ctgtgctgcg cattccgcag aatattatcc gcttgcaacc
    32641 gttgcatttt tcttacgaaa aaggccgcag agtttcaccc ctgcggcctg gtccgggcgc
    32701 aaattgccat tacggcagcc tgacgcccgc atgacacgtt acttccgctt gttcagccag
    32761 ttcacgatca tactggcaag aatgccagca atgaccggag ccgctaaatc atgccagaag
    32821 gccatgccca gttctgcgag cgtcatatag ccgcctgtat tgtaatgaca acgtttcgcg
    32881 gctattcttg tattgtttgg atgcaagaat ggcccccgtg gtgttgtcag gtgcatacct
    32941 gcaacgtgcg ggggttttct ctctccagca accaatgcca ccagggatta agcccccgca
    33001 acattgcgcc tcaccggaat ccttccggct tgacgctgat tctacgactg tttttctgtt
    33061 ccggaatccg tctcatcttc actctgtgct gcgcattccg cagaatatta tccgcttgca
    33121 accgttgcat ttttcttacg aaaaaggccg cagagtttca ctcctgcggc ctggtccggg
    33181 cgcaaattgc cattacggca gcctgacgcc cacatgacac gctacttccg cttgttcagc
    33241 cagttcacga tcatactggc aagaatgcca gcaatgaccg gagccgctaa atcatgccag
    33301 aaggccatgc ccagctctgc gagcgtcata tagccgcctg tgttgtaatg acaacgtttc
    33361 gcggctattc ttgagtggtc taggttcaag attagccccc gttatgttgt caggtacata
    33421 cctgcaacgt gcgggggttt tctctctcca gcaaccaatg ccaccaggga taaagccccc
    33481 gcaacattgc gcctcaccgg aaccctttcg gcttgccgct gattttacga ttgcttttta
    33541 ctgtcagcac ccggcaatct tcctcattgc gcgatgcctt ccagcttaat ttctttattt
    33601 gcaatagttg cagttcatat ccgggctgac tccagactgg tagtgaaatg cgcaattttg
    33661 tagaattgcg cctcctcgcg acctccccag tgaatctgtt gcctcatttt ttacatactg
    33721 cgtattcgac ttctccacct gttgcgcaag agaaactggg tttattcatt tttgcgaggc
    33781 cgacttcttt ctggacagga ctttatgcag cacaacacac tatcgaaaca caatcagaaa
    33841 ttgccgttta cacgctacga cttcggctgg gttttattat gcataggcat ggcgattggt
    33901 gccggaaccg tgctgatgcc agtacaaatt ggcttgaagg gaatttgggt atttattacc
    33961 gcagcgatca ttgcttatcc tgccacctgg gtagtgcagg acatttattt aaaaaccctt
    34021 tctgaaagcg attcctgtaa tgactacacc gatattatca gtcattacct ggggaagaac
    34081 tggggaattt tcctcggggt tatctacttt ttgatgatta tccacgggat ttttatctac
    34141 tctctctccg tggttttcga cagcgcctcg tacctgaaaa ccttcggttt aaccgatgcc
    34201 gatctttcac aatctctact ttataaagtc gctattttcg ccgtactggt ggcgattgcg
    34261 tctggtggtg aacgattact gtttaagatt tccgggccaa tggtggtggt caaagtaggg
    34321 attattgtcg tgttcggttt tgcgatgatc ccgcactgga atttcgccaa tataaccgcc
    34381 ttcccgcaag cctccgtctt tttccgcgat gtcttgctta ccattccatt ttgcttcttt
    34441 tctgcagtat ttattcaggt acttaaccca atgaatattg cctatcgtaa acgggaagcg
    34501 gataaagtac tggcaacccg gctcgcgctg cgtacccacc gaattagtta tatcacgctc
    34561 atcgcggtga tcctgttttt tgccttttcg tttaccttct caattagcca cgaagaagcc
    34621 gtttctgcct ttgaacaaaa tatctcggca ctggcgctgg ccgcgcaggt gatccctgga
    34681 catatcattc atatcacctc tacggtgctg aatatctttg ccgtactgac cgcattcttt
    34741 ggcatttatc tcggtttcca cgaggctatt aaaggcatta ttctcaatct gttaagccga
    34801 attattgata ccaagaaaat taactcacgc atgctgactc tggcgatctg cgcttttatc
    34861 gtcattacat tgacgatttg ggtttcgttt cgtgtatcgg tgctggtgtt ctttcagttg
    34921 ggaagcccgt tatatggcat tgtgtcgtgc ctcattccgt ttttcctgat ctataaagtc
    34981 gcacaactgg aaaaacttcg cggatttaaa gcctggctaa ttctgcttta cggcattttg
    35041 ctatgcttgt cgccactgtt gaagctgatt gagtaaaccg gagcgcatgg ccccggtttt
    35101 gtgagttaac gctgcggatt ttcatcctga tcaacagcaa aacaagctac cagttgaccg
    35161 ccgtagtctt ttagctgcgg ctgcaactgg gtgcaggggc cgaagcgccg acgacagcgg
    35221 gcgttgaagg cgcaacccgg cggtggattc agcgggcttg gcagttcacc ggtgagtttg
    35281 atgcgttcgc ggcgatcgtc cgggttcagg cgcggcgtcg cggagagtag cgcctgagtg
    35341 tacggatggc gcgggttatt gaagatttgg tctttcgttc ccttctccac gcagcggccc
    35401 aggtacatca ccatcacttc atcggcaatg tgctccacca ccgacaggtc gtgggagata
    35461 aagacataag aaagccccag ttcctgctgt aaatccatca tcagattcag cacttgcgca
    35521 cgcactgaga cgtccagcgc agaaaccggc tcatcggcaa tcaccacatc cgggtcgagc
    35581 atcagaccac gggcaatagc gatacgctga cgttgtccgc cggagaacat atgcggatag
    35641 cggtcatagt gctcggtttt caggccgact ttcgccatca tcgacagggc tttttcccga
    35701 cgctgttctt tgcttaagct ggtgttaata agcagcggct cttcgagaat ttgcccaact
    35761 tttttacgcg gattcagcga accgtaaggg ttctggaaga cgatctggat tttctgccga
    35821 cgcagcttct gcgcctgcgg atcgtgctta agcagatcct gcccctggta atacagctcg
    35881 ccaccggtgg gcatttcaat catcgtcagc aaccgaccga gggtcgattt accgcagcca
    35941 gattcgccca ctactgccag cgttttgcca cgttcaaggt taaacgaaac gccatccagc
    36001 gctttaacca gacgttccgg cgcgaacatg cctttcttca ccggataatg ttttttcagg
    36061 tcgatagcct gcaacagcgg ttgttgcgag gtggcctctt gcgtactcat agtgtcggcc
    36121 tcccggcatc atcaagtggg taatggcatt tggactgacg cccatcagcg agcatattca
    36181 gcgccggttc ttcagcgcga catctgtcag tggcataggg gcagcgcggg ttaagcaggc
    36241 agccgttcgg gcggtcgtac ttaccgggaa cgacacctgg caacgacgcc agacgttctt
    36301 tgtcctgagc aaattctggc agcgcacgca gcaatgcctg agtatacggg tgacgcggcg
    36361 catggaagat ggcgtgcgca tcaccggttt ccaccacctg gcctgcatac atcacgatga
    36421 ttttatgtgc cgcttccgcc accagcgcca ggtcatgggt aattaacacc agcgccatgt
    36481 tctctttctg ctgtagctcc agcagtagtt cgatgatttg cgcctgaatg gtcacgtcca
    36541 gcgcggtggt cggttcatcg gcaatcagca gttttggccg acaggcaatc gccatggcga
    36601 tcatcacgcg ctggctcatg ccgccggaaa gctgatgcgg gtaaacatcc agacgcgatg
    36661 ccggatcggg aataccgacc tgattcagca ggtcaatcgc tcgctgacgg cgggtacttt
    36721 tgttgccgcc ctgatgcacc ttaatcgctt ccataatctg gaaacccacg gtgtagcacg
    36781 ggttaaggct ggtcatcggg tcctggaaga tcatcgccac ttcggcaccc accaggttgc
    36841 ggcgctcttt ttctgagata cgctgcaaat cctggccgtt aaactccagt ttttctgcca
    36901 ttacgcggcc cggataatca atcagcccca taatcgccag tgaactgacc gacttaccgg
    36961 agccggactc acccacaatc ccgaccactt cgccctgttt tacgctgtag ctgatgcggt
    37021 ctacggcgcg gaacggcgcg ctttcgtcgc cgaaatgcac cgataattta tctacattta
    37081 ataacgccat ctcgaacctc ttactgcttc agtttgggat cgagcgcgtc acgcagaccg
    37141 tcacccatca ggttaaatgc cagcaccgtc agcaggatcg ccagacccgg gaaggtcacg
    37201 acccaccagg cgctttgcgc gaactgcaac acgtcggaga gcatggtgcc ccactcaggc
    37261 gttggcggct gtgcccccat gccgaggaaa ccaagagcag ccatatcgag aatggcgtta
    37321 gagaaaccga gcgacgcctg aacaatcagc ggcgcaaggc agttcgggaa gatgttaata
    37381 aacatctgac gcatcgcccc ggcacccgcc acgcgagacg cggtgacgta atcgcggtta
    37441 acttccacca gcacggcggc gcgggttaag cgcacatagt gcggcaaggc aacgaaggtc
    37501 agtgccagcg cggcgttacc aatcgacggg ccgaaaattg ccaccagcac cagcgccagc
    37561 agcagacttg gcagcgccag catgatatcg accacgcgca taatgatgtt atcgaccagg
    37621 ccgccaaagt aaccggcgat caggccgaga ataacgccca taatcagcga taacacaact
    37681 accagacagc caaccagcag cgacaggcgc gcaccgtaca tcaggcgcga cagcacatca
    37741 cggcctacgt catcggtgcc cagcaagtgc gccatgctgc cgccttcctg ccaggctggc
    37801 ggggcgagca gtgcatcgcg gaactgttcc gccgggttat agggtgcaat ccagttggca
    37861 aagatcgcga tgaacagcac gatgacgacg taaaccagcc cgacgaccgc gcctttgttg
    37921 cgtttaaaat agtgccagaa ctcctgtaac ggggtcatcg gcaccggtgc gctaatcact
    37981 ttattttcag taacctgtga catgatgtcc ccttacttct tatgacgaat acgcgggttc
    38041 accacgccgt acagcagatc gaccagcaag ttgacgagga taatcatcgt cgccaccagc
    38101 aatacgccgc cctgcactac cggatagtcg cggcgttgca gtgcgtcaat caaccagcgt
    38161 cccagaccgg gccacgagaa gatggtttcg gtcagaatcg cccccgccag caatgttccc
    38221 acctgcaggc cgataacggt caccaccggc agcatcgcgt tacgcagcgc atggacgata
    38281 atcacccgca tgcgggttag ccctttggcg cgcgcggtgc ggatgtaatc ctcgcccagc
    38341 acttccagca tcgaggagcg tgtcatacgc acaatgaccg ccagcggaat agtacccagc
    38401 acaatggcag gcaagatcat atgggcgacg gcatcgataa agttgccgtc ttcaccccag
    38461 atggcggtgt cgattagcat aaaaccggtt aacggattgg agtcatcgag gaacaccata
    38521 tcgctcacgc gaccggagac gggcgtcagg ttccagtgca ccgaaaccag catgatcagc
    38581 atcatgcccc accagaagat cggcatcgag tagccggtca gcgccaggcc aaccgctgtg
    38641 tgatcgaaaa tggaaccgcg tttaaccgca gccagcacac caaccggaat accgactgcc
    38701 gtagcaaaaa tcatcgcgca gacgccaagt tccagcgtgg cctgaaagcg cggcacgaac
    38761 tcttcccata ctggaattcg gcttttcatt gaaatgccta gatcgccgtg cattacgccc
    38821 caaatgtaat ggagatactg ctgccacatc ggtttatcta agccgagttc agccagcagc
    38881 tgcgcgtgac gctcagggga gatcccacgt tcgcctgcca tgatcatcac cggatcgccc
    38941 gggatcatgt ggacaaaggc aaatgtgaga agggtaatac cgataaacgt ggggatgacg
    39001 agtcccaaac gtcggagaat aaactgcaac ataacccgga ttctctatag tgacccgcag
    39061 cctggaactg cgtgtctgta ttgctcacaa atcaattccc gcccttgtcg gacagagaat
    39121 gaagtattgc tgccggatgg cgctgcacgc agaggcacag cgccaccggg tatacagatt
    39181 gttgacaaag tgcgctttgt ttatgccgga tgcggcgtta acgccttatc cggcctacaa
    39241 aatcgtgcaa attcaataca ttgcagaatt ttcgtaggcc tgataagcgg agcacatcag
    39301 gcagttttgc ctttgccatc agtcttgtat ggcttttaat tattcgatag agacgttttc
    39361 gaagtgatgt ttgcctaatg gatcaaccac atagcctttg acttctttgc gtaccggttc
    39421 aaacacggtg gagtgagcga tgatcagtgc cggagcctga tcgtgcatca ccacctgcgc
    39481 ttgcttgtac agttcaacgc gtttgttatg gtcgtcggta gcacgcgccg gttgaatcag
    39541 atcttcaaac ggtttgtagc accattttga gtagttagaa ccttgttcag aggcggcgca
    39601 gctgaacagg gtggcgaaga agttatccgg gtccccgtta tcgccagtcc agcccatcat
    39661 taccgtctga tgttcgccat ctttcgcacg cttgaggtac tcaccccatt cgtaggtgac
    39721 gattttggcc tgcacgccca ctttcgccca gtctgcctga atcatctccg ccatgcggcg
    39781 agcgttcggg ttatacggac gttgtaccgg catcgcccac aggtcgatgg agaaaccttt
    39841 ttccagaccc gcttctttca gcaaggcttt cgctttttca ggatcgtagg tgtaatcctg
    39901 aacgtcgtcg ttatagcccc acatggttgg cgggatcagg tttttcgctg atacacccgc
    39961 gccctgataa accgctttga tgatcgcgtc tttgttcacc gcgtaggtca gtgcctggcg
    40021 aactttcacg tcatccagcg gttttttctg cacgttatac gagaggtagc cgacgttcag
    40081 ccccggcatt tccatcaggt tgatggattt atcctgcttc atgcgagcga tatctgccgg
    40141 gttcgggtac ggcatcacct ggcattcatt cttctgcaat ttcgcgtaac gcacggaagc
    40201 atcaggggta atagagaaga ccagcgtatc gatctgcggt ttggtgcccc agtagccatc
    40261 aaacgctttg tagcggatac gggagtcttt ttggtactgc tgtaactgga acggaccggt
    40321 tccgattggg ttgaggtcca gtttttccgg tgtaccggct ttcatcatcg catcagcata
    40381 ttcttttgac agaatagagg cgaagtccat tgccagatca gcgaggaacg gcgcttccgg
    40441 gcgagtcagc acgaactgaa ctgtgttgtc gtccaccttt ttcacttcgc tgatcagctc
    40501 cggcaagccc atgccttcga agtattcgta gctaccgcca gaaactttat ggtacgggtt
    40561 ttgcgcgttt ttctgacgat cgaacgagaa caccacgtca tcggcgttca gttcgcgcgt
    40621 cggtttgaat tctttattgt catgccactt cacaccttta cgcagatgga aggtataggt
    40681 tttaccgtct tcgctgactt cccacttttc agcgaggccc gggatcactt cagtggtgcc
    40741 gattttaaat tcaaccaggc ggttataaag cggtacggaa gaggcgtcat aggtggtgcc
    40801 ggaggtaaac agctgcgggt taaacccttc cggagatcct tctgagcaat aaaccagagt
    40861 tttagcctga acacttgctg cgacggtcat agccaccagg ctgagaccaa gcttcagcat
    40921 ccctgacttt ttcaaggaaa tacgcattat tctgctccaa ttgtgatgtt tgttgtttta
    40981 accctttgca gtgggttgtc gctgcctgac cttttttgtt ttttgcccgg tcgggtcaac
    41041 gttatgaggt ggggatgccg tactaattaa catcagtgta acagtgcgga tcctccataa
    41101 aatgcccctc gtgacctaca atctgtcaac agaatgtgaa aacgtcaata cagccaaccg
    41161 ggatttacac caacggtgag aatccacaaa caaagattaa aaaaacttca aacagctatt
    41221 tgcagcagag aaatttgtgc tactccaaca tgaccagaac aatca
//
LOCUS       DAPPNJ010000012        40876 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000012 DAPPNJ010000000
VERSION     DAPPNJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.012"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            123..458
                     /gene="eutS"
                     /locus_tag="RPM73_000479"
     CDS             123..458
                     /gene="eutS"
                     /locus_tag="RPM73_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDX6518874.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            471..950
                     /gene="eutP"
                     /locus_tag="RPM73_000480"
     CDS             471..950
                     /gene="eutP"
                     /locus_tag="RPM73_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDX6518875.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            925..1626
                     /gene="eutQ"
                     /locus_tag="RPM73_000481"
     CDS             925..1626
                     /gene="eutQ"
                     /locus_tag="RPM73_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDX6518876.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1623..2426
                     /gene="eutT"
                     /locus_tag="RPM73_000482"
     CDS             1623..2426
                     /gene="eutT"
                     /locus_tag="RPM73_000482"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDX6518877.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2423..3439
                     /gene="pta"
                     /locus_tag="RPM73_000483"
     CDS             2423..3439
                     /gene="pta"
                     /locus_tag="RPM73_000483"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDX6518878.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            3478..3771
                     /gene="eutM"
                     /locus_tag="RPM73_000484"
     CDS             3478..3771
                     /gene="eutM"
                     /locus_tag="RPM73_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDX6518879.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3878..4165
                     /gene="eutN"
                     /locus_tag="RPM73_000485"
     CDS             3878..4165
                     /gene="eutN"
                     /locus_tag="RPM73_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDX6518880.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4177..5580
                     /locus_tag="RPM73_000486"
     CDS             4177..5580
                     /locus_tag="RPM73_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDX6518881.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5591..6427
                     /gene="eutJ"
                     /locus_tag="RPM73_000487"
     CDS             5591..6427
                     /gene="eutJ"
                     /locus_tag="RPM73_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDX6518882.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6417..7604
                     /gene="eutG"
                     /locus_tag="RPM73_000488"
     CDS             6417..7604
                     /gene="eutG"
                     /locus_tag="RPM73_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDX6518883.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7721..8947
                     /gene="eutH"
                     /locus_tag="RPM73_000489"
     CDS             7721..8947
                     /gene="eutH"
                     /locus_tag="RPM73_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDX6518884.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8944..10347
                     /gene="eutA"
                     /locus_tag="RPM73_000490"
     CDS             8944..10347
                     /gene="eutA"
                     /locus_tag="RPM73_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDX6518885.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10359..11720
                     /gene="eutB"
                     /locus_tag="RPM73_000491"
     CDS             10359..11720
                     /gene="eutB"
                     /locus_tag="RPM73_000491"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDX6518886.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11741..12628
                     /gene="eutC"
                     /locus_tag="RPM73_000492"
     CDS             11741..12628
                     /gene="eutC"
                     /locus_tag="RPM73_000492"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDX6518887.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12638..13297
                     /gene="eutL"
                     /locus_tag="RPM73_000493"
     CDS             12638..13297
                     /gene="eutL"
                     /locus_tag="RPM73_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDX6518888.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13310..13810
                     /gene="eutK"
                     /locus_tag="RPM73_000494"
     CDS             13310..13810
                     /gene="eutK"
                     /locus_tag="RPM73_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDX6518889.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13856..14908
                     /gene="eutR"
                     /locus_tag="RPM73_000495"
     CDS             13856..14908
                     /gene="eutR"
                     /locus_tag="RPM73_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDX6518890.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14914..15813)
                     /gene="hemF"
                     /locus_tag="RPM73_000496"
     CDS             complement(14914..15813)
                     /gene="hemF"
                     /locus_tag="RPM73_000496"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDX6518891.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15817..16686)
                     /gene="amiA"
                     /locus_tag="RPM73_000497"
     CDS             complement(15817..16686)
                     /gene="amiA"
                     /locus_tag="RPM73_000497"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDX6518892.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16900..17325
                     /locus_tag="RPM73_000498"
     CDS             16900..17325
                     /locus_tag="RPM73_000498"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDX6518893.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17312..17761
                     /locus_tag="RPM73_000499"
     CDS             17312..17761
                     /locus_tag="RPM73_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDX6518894.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17822..18397
                     /locus_tag="RPM73_000500"
     CDS             17822..18397
                     /locus_tag="RPM73_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDX6518895.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18493..19392
                     /gene="yfeX"
                     /locus_tag="RPM73_000501"
     CDS             18493..19392
                     /gene="yfeX"
                     /locus_tag="RPM73_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDX6518896.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19450..20754)
                     /gene="pbp4b"
                     /locus_tag="RPM73_000502"
     CDS             complement(19450..20754)
                     /gene="pbp4b"
                     /locus_tag="RPM73_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDX6518897.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20759..22183)
                     /gene="murP"
                     /locus_tag="RPM73_000503"
     CDS             complement(20759..22183)
                     /gene="murP"
                     /locus_tag="RPM73_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDX6518898.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22187..23083)
                     /gene="murQ"
                     /locus_tag="RPM73_000504"
     CDS             complement(22187..23083)
                     /gene="murQ"
                     /locus_tag="RPM73_000504"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDX6518899.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23247..24104
                     /gene="murR"
                     /locus_tag="RPM73_000505"
     CDS             23247..24104
                     /gene="murR"
                     /locus_tag="RPM73_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDX6518900.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24233..25024
                     /gene="ucpA"
                     /locus_tag="RPM73_000506"
     CDS             24233..25024
                     /gene="ucpA"
                     /locus_tag="RPM73_000506"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDX6518901.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25195..26211
                     /gene="cysP"
                     /locus_tag="RPM73_000507"
     CDS             25195..26211
                     /gene="cysP"
                     /locus_tag="RPM73_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDX6518902.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26211..27044
                     /gene="cysT"
                     /locus_tag="RPM73_000508"
     CDS             26211..27044
                     /gene="cysT"
                     /locus_tag="RPM73_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDX6518903.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27044..27919
                     /gene="cysW"
                     /locus_tag="RPM73_000509"
     CDS             27044..27919
                     /gene="cysW"
                     /locus_tag="RPM73_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDX6518904.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27909..29006
                     /gene="cysA"
                     /locus_tag="RPM73_000510"
     CDS             27909..29006
                     /gene="cysA"
                     /locus_tag="RPM73_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDX6518905.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29140..30051
                     /gene="cysM"
                     /locus_tag="RPM73_000511"
     CDS             29140..30051
                     /gene="cysM"
                     /locus_tag="RPM73_000511"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDX6518906.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30054..30422)
                     /locus_tag="RPM73_000512"
     CDS             complement(30054..30422)
                     /locus_tag="RPM73_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDX6518907.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30527..31378
                     /gene="pdxK"
                     /locus_tag="RPM73_000513"
                     /gene_synonym="thiJ"
     CDS             30527..31378
                     /gene="pdxK"
                     /locus_tag="RPM73_000513"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDX6518908.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31420..31929)
                     /gene="crr"
                     /locus_tag="RPM73_000514"
     CDS             complement(31420..31929)
                     /gene="crr"
                     /locus_tag="RPM73_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDX6518909.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31970..33697)
                     /gene="ptsI"
                     /locus_tag="RPM73_000515"
     CDS             complement(31970..33697)
                     /gene="ptsI"
                     /locus_tag="RPM73_000515"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDX6518910.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33742..33999)
                     /gene="ptsH"
                     /locus_tag="RPM73_000516"
     CDS             complement(33742..33999)
                     /gene="ptsH"
                     /locus_tag="RPM73_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDX6518911.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34383..35354)
                     /gene="cysK"
                     /locus_tag="RPM73_000517"
     CDS             complement(34383..35354)
                     /gene="cysK"
                     /locus_tag="RPM73_000517"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDX6518912.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35539..36300)
                     /gene="cysZ"
                     /locus_tag="RPM73_000518"
     CDS             complement(35539..36300)
                     /gene="cysZ"
                     /locus_tag="RPM73_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDX6518913.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36530..37516
                     /gene="zipA"
                     /locus_tag="RPM73_000519"
     CDS             36530..37516
                     /gene="zipA"
                     /locus_tag="RPM73_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDX6518914.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37587..39602
                     /gene="ligA"
                     /locus_tag="RPM73_000520"
     CDS             37587..39602
                     /gene="ligA"
                     /locus_tag="RPM73_000520"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDX6518915.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39604..39822
                     /locus_tag="RPM73_000521"
     CDS             39604..39822
                     /locus_tag="RPM73_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDX6518916.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39819..40817)
                     /locus_tag="RPM73_000522"
     CDS             complement(39819..40817)
                     /locus_tag="RPM73_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDX6518917.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
BASE COUNT         9499 a        10520 c        11502 g         9355 t
ORIGIN      
        1 cagcgaatat ctctccattc ttagtgatct acctcacctt ttaaacgcgc ttgccgaatt
       61 ttgttattta ctctgacgaa aaattgtcac gatacacgaa agtttttcac aggcggcgac
      121 tcatggataa agaacgcatc attcaggaat ttgtgcccgg caaacaggtc acactggcgc
      181 atctcatcgc gcatcccggt gaggaactgg cgaagaaaat cggcgttccg gatgcgggcg
      241 caattggcat tatgacgcta actcccggcg aaacggcgat gatcgccggt gatttagcgc
      301 tgaaagccgc tgatgtgcat atcggttttc tcgaccgctt cagcggtgca ttggtgattt
      361 atggctcggt gggcgcagta gaagaagcgt tatcacaaac cgtcagcggt ttaggtcgct
      421 tattaaatta tacgctttgt gaaatgacca aaagttaata cgaggcattc atgaaacgaa
      481 ttgcgtttgt gggtagcgtc ggggcaggaa aaacaacgtt atttaatgcg ttgcagggga
      541 attataccct cgccagaaaa acacaggccg tggaatttaa tgataagggc gatattgata
      601 cgccgggtga atattttagc catccccgct ggtatcacgc cttaattacc acgctacagg
      661 atgtagacat gctgatctat gtccatggcg caaatgaccc ggaaagtcgg ttgcctgccg
      721 ggttgctgga tattggcgtc agcaaacggc aaatcgccgt catcagtaaa acggacatgc
      781 cagatgctga cgtcgccgca acgcgaaagt tattgcttga gacgggtttc gaagagccga
      841 ttttcgagct aaacagccac gacccgcaaa gcgtgcagca actggtggat tatctggcat
      901 ctctcaccaa acaggaggaa gcaggtgaaa aaactcatca cagcgaatga tattcgtgaa
      961 gcacacgcgc gcggcgaaca ggcaatgtcc gtggttctgc gcgccagcat tattaccccg
     1021 gaagcccgtg aagtggcgga tctactgggt tttaccatca ccgaatgtga tgaatcgatc
     1081 ccggtaacgg cgtccgtgcc cgccagcgtg ccagccgaca aaaccgaaag ccagcgcatc
     1141 cgcgaaacca tcattgccca gctgccggaa ggccagttta ccgaaagcct ggtcgcacag
     1201 ctgatggaaa aagtgatgaa ggaaaaacag tcgctggagc agggcgcaat gcagccgagc
     1261 ttcaaatcgg tgaccggcaa aggcggcatc aaagtgattg atggcagtag cgtcaagttt
     1321 ggtcgtttcg acggcgcaga gccgcattgc gtgggcttga ctgatcttgt gaccggagac
     1381 gacggcagca gcatggccgc cggttttatg cagtgggaga acgcattctt cccgtggacc
     1441 ctgaactacg acgaaatcga catggtgctg gaaggggagc tgcatgttcg ccacgaaggc
     1501 caaaccatga tcgccaaagc aggcgacgtg atgtttatcc cgaaaggctc cagcatcgaa
     1561 tttggtacga catccagcgt gaaattcctg tatgtcgcct ggccggctaa ctggcaatcc
     1621 ctatgaaaga tttcatcacc gaagcatggc taagagcgaa ccatacgctc agcgaaggcg
     1681 cagagatcca tctccctgcg gacagtcgcc tgacgccctc tgcccgggag ttgctggaaa
     1741 gccgccatct gcgcatcaag tttattgacg agcagggccg cctgtttgtt gacgatgaac
     1801 agcagcagcc gcagcccgtt catgggctga ccagtagcga tgaacatccg caggcgtgct
     1861 gcgaactgtg tcgccagccg gtggcaaaaa agccggatac gctgactcac ctgtcggcgg
     1921 aaaaaatggt cgccaaaagc gatccgcgtc tgggttttcg cgcggttctc gacagcacca
     1981 ttgcgctggc agtgtggctg caaattgaac tggcggaacc gtggcagccg tggctagcgg
     2041 atatccgttc gcgtcttggc aacattatgc gcgccgatgc gctgggggaa ccgctgggct
     2101 gccaggcgat tgtcgggctt tctgacgaag atctgcatcg gctttctcat cagccgctgc
     2161 gctatctcga ccacgatcat ctggtgccgg aagccagcca cgggcgcgat gcggcgttgc
     2221 tcaatctgct acgtaccaaa gtgcgggaaa ccgaaaccgt agcggcgcag gtgtttatca
     2281 cccgcagctt tgaagtatta cgcccggaca ttttgcaggc gctgaaccgc ctttcgagca
     2341 cggtctacgt gatgatgatc ctgagcgtaa cgaagcagcc gctaacggtg aagcagatcc
     2401 aacaacgact gggggaaacg caatgattat tgaacgttgt cgtgaactgg cgttgcgagc
     2461 gcccgccaga gtggtttttc cggatgcgtt agaccaacgt gtgctgaaag ccgcgcaata
     2521 tttacatcaa caaggtctgg caacgcccat tctggtcgcc aatccgtttg aacttcgtca
     2581 gtttgcgctc agtcacggcg tggcgatgga cgggctacag gtgattgatc cgcatggcaa
     2641 cctcgcaatg cgggaagaat ttgctcatcg ctggctggcc cgcgcgggcg aaaaaacgcc
     2701 gccggatgcg ctggaaaaac ttaccgaccc tctgatgttc gccgcagcaa tggtcagcgc
     2761 cggtaaagcg gatgtctgta tcgcgggcaa cctctcttcc acggcaaatg tgctgcgtgc
     2821 cggattacgc attatcggct tgcagccagg ctgtaaaacg ctctcttcca ttttcctgat
     2881 gttgccacag tacagcggcc cggcgttggg ctttgccgat tgcagcgtgg tgccacagcc
     2941 gacggcggcg cagctggcgg atatcgcgct tgccagtgcc gaaacctggc gcgccatcac
     3001 cggagaagag ccgcgcgtgg cgatgctgtc gttttccagt aacggtagcg cccgtcaccc
     3061 ctgcgttgcc aacgtccagc aggcgacaga aatcgtccgt gagcgcgcac caaagctggt
     3121 ggttgatggc gagttacagt ttgacgccgc cttcgtgccg gaagtggcgg cgcaaaaagc
     3181 gcctgccagc ccgctacagg gcaaggccaa tgtgatggtt tttccgtcgc tggaagccgg
     3241 aaatattggt tacaaaatcg cacaacgact cggcggatat cgtgccgtcg ggccactgat
     3301 acaaggactt gccgcgccga tgcacgatct ctctcgtggt tgtagcgtgc aggaaattat
     3361 cgagctggcg ctggtggcag ctgtgccgcg tcagacaaaa gtgaaccgcg aaagcagttt
     3421 acaaacactg gttgaatgaa cggtcccgtt ctggaccctg ataaagagga aagcacaatg
     3481 gaagcattag gaatgatcga aacccggggc ctggttgcac tgattgaggc ttctgacgcg
     3541 atggtgaaag ctgcgcgcgt caagctggtt ggtgttaagc agattggcgg cggcctgtgt
     3601 actgccatgg ttcgtggtga tgttgcagcc tgtaaagccg cgaccgatgc aggtgccgca
     3661 gcggcacagc gtatcggcga gctggtatct gtacacgtta tcccgcgtcc gcacggcgac
     3721 ctggaagaag tcttcccgat cggcctgaaa ggcgatagca gcaacctgta atcaccctgt
     3781 gtgacgtcga ttcgggcgtc acgctctctc ctttttcatt ttaccttctg cggagggtag
     3841 ggggagcttt tacctgaaat acgccacgga ggcgggtatg aaactggcag tcgtcactgg
     3901 acaaattgtt tgtaccgtac gccatcacgg actggcgcat gacaaattgc tgatggtgga
     3961 aatgattgat ccacaaggta atcctgacgg gcaatgcgcc gtcgccatcg acaatattgg
     4021 cgcgggaacc ggggagtggg tgttactggt gagtggcagt tccgcccgcc aggcgcataa
     4081 aagcgaaacg tcaccggtcg atctgtgcgt gattggcatt gtcgatgagg tggtgtctgg
     4141 cggtcaggta attttccaca aataaggcag aacatcatga atcaacagga tattgaacag
     4201 gtggtgaaag cggtactgct gaaaatgcaa agcagtgaca cgccgtccgc cgccgttcat
     4261 gagatgggcg ttttcgcgtc cctggatgac gccgttgcgg cagccaaagt cgcccagcaa
     4321 gggttaaaaa gcgtggcaat gcgccagtta gccattgctg ccattcgtga ggcaggcgaa
     4381 aaacacgcca gagatttagc ggaacttgcc gtcagtgaaa ccggcatggg ccgcgttgaa
     4441 gataaatttg ccaaaaacgt cgctcaggcg cgcggcacgc caggcgttga gtgtctctct
     4501 ccgcaggtgc tgaccggcga caacggcctg actttaattg aaaacgcgcc ctggggcgtg
     4561 gtggcttcgg taactccgtc cactaacccg gcggcaaccg taattaataa cgccatcagc
     4621 ctgattgccg cgggcaacag cgtcattttt gccccgcatc cggcggcgaa aaaagtctcc
     4681 cagcgggcga ttacgctgct caaccaggcg attgttgccg caggtgggcc ggaaaactta
     4741 ctggttactg tggcaaatcc ggatatcgaa accgcgcaac gcttgttcaa gtttccgggt
     4801 atcggcctgc tggtggtgac cggcggcgaa gcggtagtag aagcggcgcg taaacacacc
     4861 aataaacgtc tgattgccgc aggcgctggc aacccgccgg tagtggtgga tgaaaccgcc
     4921 gacctcgccc gtgccgctca gtccatcgtc aaaggcgctt ctttcgataa caacatcatt
     4981 tgtgccgacg aaaaggtact gattgttgtt gatagcgtag ccgatgaact gatgcgtctg
     5041 atggaaggcc agcacgcggt gaaactgacc gcagaacagg cgcagcagct gcaaccggtg
     5101 ttgctgaaaa atatcgacga gcgcggaaaa ggcaccgtca gccgtgactg ggttggtcgc
     5161 gatgcggcga aaatcgcggc ggcaatcggc ctgaacgtgc cgcaagaaac gcgtttgctg
     5221 tttgtggaaa ccactgcaga acatccgttt gccgtgaccg aactgatgat gccggtgctg
     5281 cccgtcgtgc gcgtcgccaa cgtggcggat gccattgcgc tggcggtgaa actggaaggc
     5341 ggttgccacc acacggcggc aatgcactcg cgcaacatcg aaaacatgaa ccagatggcg
     5401 aatgctattg ataccagcat tttcgttaag aacggaccgt gcattgccgg gctggggctg
     5461 ggcggggaag gctggaccac catgaccatc accacgccaa ccggtgaagg ggtaaccagc
     5521 gcgcggacgt ttgtccgtct gcgtcgctgt gtattagtcg atgcgtttcg cattgtttaa
     5581 ggagtaaccg atggcgcacg acgaacaatg gctcacccca cgtctgcaaa cggcggcaac
     5641 gctgtgtaac cagacgcctg ccgcgacgga atctccgctg tggctggggg tggatctggg
     5701 cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt cagccggtgg cggtatgcct
     5761 cgactgggcc gatgtggtcc gcgacggcat cgtctgggat ttcttcggcg ctgtcaccat
     5821 tgttcgtcgc catctcgaca cgctcgagca gcaattcggt cgccgtttta gccatgcggc
     5881 gacttcattt cctccaggta cggacccgcg tatttccatc aacgtgctgg aatctgccgg
     5941 gctggaagtg agccatgtgc tggatgagcc aaccgcagtc gcggatctgc tgcaactgga
     6001 caacgccggt gtagtggata tcggcggcgg cactaccggc attgccatcg tgaaaaaggg
     6061 caaagtgacg tactcggcgg atgaagccac cggcggacat cacatctctc tgactctcgc
     6121 cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac aagcgcggtc acggtgaaga
     6181 gatttggcct gcggtgaaac cggtgtacga aaaaatggcg gacattgtcg cccggcatat
     6241 tgaagggcag ggcattactg atttatggct ggcgggcggc tcctgtatgc aaccgggcgt
     6301 ggcggaactg tttcgcaaac aattcccggc gttacaagtg catttaccgc agcacagttt
     6361 gtttatgacc ccgctggcga tcgccagtag tgggcgagag aaagcggagg ggctatatgc
     6421 aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac tctgaatctg caacgggtaa
     6481 aaacatttag cgttccaccg gtgacgcttt gcggtccggg ctcggtgagc agttgcggac
     6541 agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat ggcagacagc tttttgcatc
     6601 aggcagggat gaccgccggg ctgacgcgca gcctggctgt taaaggcatc gccatgacgc
     6661 tctggccatg tccggtgggc gaaccgtgca tcaccgacgt gtgtgcagcc gtggcgcagt
     6721 tgcgtgagtc aggctgtgat ggggtgatcg catttggcgg cggctcggtg ctggatgcgg
     6781 cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac gctggcagag atgtcagaaa
     6841 ccagcgttct gcaatcgcgc ctgccgttga ttgccattcc cactaccgcc ggaaccggct
     6901 ctgaaaccac caacgtaacg gtgattatcg atgcggtgag cgggcgtaag caggtgttag
     6961 cccatgcctc gctgatgccg gatgtggcga ttctcgacgc cgcattgacc gaaggtgtgc
     7021 cgtcgcatgt cacggcgatg accggcattg atgcgttaac ccatgccatt gaagcataca
     7081 gcgccctgaa cgctacaccg tttaccgaca gtctggcgat tggtgccatt gcgatgattg
     7141 gcaaatcgct gccgaaagcg gtgggctacg gtcatgacct tgccgcgcgc gagagcatgt
     7201 tactggcttc atgtatggcg ggaatggcgt tttccagtgc gggtcttggg ttgtgccacg
     7261 cgatggcgca tcagccaggg gcggcgctgc atattccaca cggtctggcg aacgccatgt
     7321 tgctgccaac ggtgatggaa tttaaccgga tggtttgtcg cgaacggttt agtcagattg
     7381 gtcgggcact gcgaactaaa aaatccgacg atcgtgacgc tattaacgcg gtaagtgagc
     7441 tgattgcgga agttgggatt ggtaaacgac tgggcgatgt tggcgcgaca tctgcgcatt
     7501 acggtgcatg ggcgcaggcc gcgcaggaag atatttgtct gcgcagcaac ccgcgtaccg
     7561 ccagcctgga gcagattgtc ggcctgtacg cagcggcgca ataaatgccg gatgcgacgc
     7621 ttgccgcgtc tgatccggcc ttcgggattg cacatgtagg gcggataagg cgtttacgcc
     7681 gcatccgcca ataaataaca actaacaggg agtaaaggcg atgggaatta acgaaatcat
     7741 catgtacatc atgatgttct ttatgctgat agctgccgta gacaggatcc tgtcgcagtt
     7801 cggcggttct gctcgtttcc tcggtaagtt cggtaaaagt atcgaaggat caggcggtca
     7861 gttcgaagaa ggctttatgg caatgggcgc actgggcctg gcgatggtcg gtatgaccgc
     7921 gctggcaccg gtactggctc acgtactcgg gccggtaatt attccggttt acgaaatgct
     7981 cggcgctaac ccatcgatgt tcgccggaac actgctggcg tgcgatatgg gcggcttctt
     8041 cctcgccaaa gagctggcgg gcggcgacgt agcagcgtgg ctatactctg ggttaattct
     8101 cgggtcgatg atggggccaa cgattgtgtt ttccattccg gtggcgctcg gcattatcga
     8161 accttctgac cgtcgttatc tggcgctcgg cgtgctggcg ggcattgtga ccattccgat
     8221 tggctgtatt gctggtggtc tggttgctat gtactccggt gtgcagatca acggtcagcc
     8281 ggtggaattc actttcgccc tgatcctgat gaacatgatc ccggtgatca ttgtcgcgat
     8341 tctggtggcg ctggggctga aattcatccc ggaaaaaatg atcaacggct tccagatctt
     8401 cgccaaattc ctcgttgcat tgatcaccct cggtcttgcc gctgcggtag tgaaattcct
     8461 ccttggctgg gaactgatcc ccggactgga tcctatcttt atggcccctg gcgataaacc
     8521 cggtgaagtg atgcgcgcca ttgaagttat cggttctatc tcctgcgtgc tgttaggggc
     8581 gtatccaatg gtgctgctgc tgactcgctg gtttgaaaaa ccgctgatga gcgtcggtaa
     8641 agtgctgaat atgaacaaca tcgcggcagc cggcatggtg gcaacgcttg ccaacaacat
     8701 cccgatgttc ggcatgatga agcagatgga tacccgcggc aaagtcatca actgcgcctt
     8761 cgccgtttcc gctgctttcg ccctgggcga ccacttaggc ttcgccgctg ccaacatgaa
     8821 cgccatgatc ttcccgatga ttgtcggcaa gttgatcggc ggcgtaacgg cgattggcgt
     8881 ggcgatgatg ctggtgccaa aagaagacgc gaccgcgact aaaaccgaag cggaggcaca
     8941 atcgtgaaca ctcgccagct attgagcgtc ggtatcgata tcggcaccac caccacccag
     9001 gtgattttct cccacctgga gctggttaac cgtgcggcgg tgtcgcaggt gccgcgctac
     9061 gaattcatta aacgcgaaat tagctggcaa agtccggtgt tctttacccc tgtcgataaa
     9121 cagggcggtt taaaagaagc ggaactgaaa accttaatac tcgagcaata tcaggctgcg
     9181 ggtattgcgc cggaaagcgt tgattctggt gccatcatca tcaccggtga aagcgcgaaa
     9241 acccgcaatg ctcgcccggc ggtgatggcg ctctctcaat cgctggggga ttttgtcgtt
     9301 gccagcgccg ggccgcacct cgaatccgtg atcgccggtc acggagctgg ggcgcaaacc
     9361 ctttctgaac aacggctgtg tcgggtactg aatatcgaca tcggcggtgg caccgcgaac
     9421 tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct gtctcaacgt cggtggtcgc
     9481 ctgctggaaa ccgacagcca ggggcgcgtg gtttacgctc ataaaccggg gcagatgatt
     9541 gtggatgagt gcttcggtgc aggcactgac gcccgttcgc tgaccggcgc gcagctggtg
     9601 caggttaccc ggcggatggc ggcgctgatt gtcgaagtga ttgacggaac gctttcgccg
     9661 ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag gtgttacgcc cgaaatcatt
     9721 acgctttctg gcggcgtggg cgaatgttat cgccaccagc ccgccgaccc gttctgtttt
     9781 gccgatattg gcccgctgct ggcaacggcg ctgcatgacc atccgcgcct gcgtgagatg
     9841 aacgtgcagt ttccggcgca aaccgtgcgt gccacggtga ttggcgcggg ggcacatacc
     9901 ctttcgctct ctggcagcac aatctggctt gagggcgtac aactgccgct gcgcaatttg
     9961 ccggtggcga tcccgattga tgaaacggat ctggtgagtg cctggcaaca ggcgctgatt
    10021 caactggatc ttgatcccaa aactgacgcg tacgtgctgg cgcttcccgc ctcgctgcct
    10081 gtgcgttacg ccgcggtact gacggtcatc aacgcgttgg tcgatttcgt cgcgcgtttt
    10141 ccgaatccgc atcccctgct ggtggtggcc gggcaggact ttggtaaagc tctgggcatg
    10201 ttgttgcgcc cacagctaca acaactcccg ttggcagtca ttgacgaagt gattgtccgc
    10261 gcgggggact atatcgacat tggtacgcct ctttttggcg gatcggttgt gccggtgacg
    10321 gtgaaatcac tcgcatttcc ttcctgaggg aacgacttat gaaactaaag accacattgt
    10381 tcggcaatgt atatcagttt aaggatgtaa aagaggtgct ggctaaagcc aacgaactgc
    10441 gttcggggga tgtgctggcg ggcgttgcag cggcaagctc acaggagcgc gtggcggcaa
    10501 agcaggtgtt gtcggaaatg accgtagcgg acatccgcaa taatccggtg attgcctatg
    10561 aagatgactg cgtgacgcgg ctgattcagg acgatgttaa cgaaacggcc tacaaccaga
    10621 ttaaaaactg gagcatcagc gaactgcgtg agtatgtgct gagcgatgaa accagcgtgg
    10681 acgacattgc ctttacccgc aaagggctga cctcggaagt ggtcgcggcg gtagcgaaga
    10741 tttgctccaa cgcggacctg atctacggcg cgaagaaaat gccggtaatc aaaaaggcca
    10801 ataccaccat tggtattccg ggcaccttca gcgcccgttt gcagccgaat gatacccgtg
    10861 acgacgtgca aagtatcgct gcgcaaatct atgaagggct ttcctttggg gtgggcgatg
    10921 cggtgatcgg cgttaacccg gtaactgacg acgtggaaaa cttaagccgc gtgttggaca
    10981 ccatttacgg cgtgatcgac aaattcaaca tcccaactca gggctgcgta ctggcgcacg
    11041 tcaccaccca gatcgaagcg attcgtcgcg gcgcgcctgg cggactgatt ttccagagta
    11101 tctgtggcag cgaaaaaggg ctgaaagagt ttggcgtgga actggcgatg ctcgacgaag
    11161 cgcgcgcagt gggtgcagag ttcaaccgta tcgccgggga aaactgcctc tacttcgaaa
    11221 ccggacaagg ctctgcgcta tccgctggcg ctaacttcgg cgctgaccag gtgacgatgg
    11281 aagcacgtaa ctatgggctg gcgcgtcatt acgatccgtt tatcgtcaac accgtggtcg
    11341 gctttattgg gccggagtat ctctacaacg accgccagat tatccgtgct ggcttagaag
    11401 atcactttat gggcaagctg agcggcatct ctatgggctg tgactgctgt tacaccaacc
    11461 acgctgacgc tgaccagaac ctcaacgaaa acctgatgat cctgctcgcc accgcaggct
    11521 gcaactacat catggggatg ccgctgggtg atgacatcat gctcaactac cagaccaccg
    11581 cattccacga taccgccact gtgcgtcagt tactcaacct gcgtccgtca ccggagtttg
    11641 aacgctggct ggaaagcatg ggcattatgg caaacggtcg cctgaccaaa cgggcgggcg
    11701 atccgtcact gttcttctga tgacgcggag ataacacatc atggatcaaa aacagattga
    11761 agaaattgta cgcagcgtga tggcgtcaat gggacaaacg gcccccgcgc cgtcagaagc
    11821 aaagtgcgcc accaccaact gtgcggcacc ggtgacctcg gaaagctgtg cgctggattt
    11881 aggttccgct gaagcaaaag cgtggattgg tgttgaaaac ccgcatcgcg cagacgtatt
    11941 aacagaactg cgccgcagca ccgtggcccg cgtttgtacc ggtcgtgccg gtccgcgtcc
    12001 acgtacccag gcgctgctgc gtttcctggc cgatcactct cgttcgaaag ataccgtact
    12061 gaaagaagtg ccggaagagt gggtgaaagc gcagggcttg ctggaagtac gctctgaaat
    12121 tagcgacaaa aatctctacc tgactcgccc ggatatgggc cgccgcctgt gtgcagaagc
    12181 tgttgaagcg ctgaaagcac agtgtgttgc taatccggac gtacaggtcg ttatttctga
    12241 tggcctgtca actgatgcca tcaccgtcaa ctacgaagag atcctgccgc cgctgatggc
    12301 gggcctgaaa caggccgggc tgaaagtcgg cacgccattc tttgttcgtt atggtcgcgt
    12361 gaagattgaa gatcagatcg gtgagatcct cggcgcgaaa gtggtgatcc tgctggtggg
    12421 ggaacgtccg gggttgggtc agtcagaaag cctctcctgt tacgccgttt actcgccgcg
    12481 tatggcgacc accgttgagg ccgatcgcac ctgcatctct aacattcacc agggcggcac
    12541 gccgccggtt gaagccgcag ccgtcattgt ggatttggcg aaacgtatgc tggagcagaa
    12601 agcatccggc atcaacatga cccgataagg aggcatcatg ccagctttag atttgattcg
    12661 accgtcggta accgccatgc gggtgattgc ctccgttaac gctgaatttg cgcgtgaact
    12721 aaaattgccg ccgcatattc gtagcctcgg gctgatttct gctgattccg atgacgtcac
    12781 ctacattgcc gctgatgaag cgaccaagca ggcgatggtg gaagtggtat atggtcgctc
    12841 gctgtatgcg ggcgctgcac acggcccgtc accgactgcc ggtgaagtgc tgattatgct
    12901 tggtgggcca aacccggcgg aagtgcgtgc cggtctggat gcgatggttg cacatattga
    12961 aaatggtgca gcattccagt gggccaatga cgcagaaaac accgccttcc tggcgcatgt
    13021 ggtttcgcgt accggttctt atctctcatc aaccgccggg atcacgcttg gcgatccgat
    13081 ggcatatctg gtggcaccgc cgctggaagc gacctatggc attgatgcag cgttgaaatc
    13141 tgccgacgta cagctggtga cctatgtccc gccaccgtct gaaaccaact actcggcagc
    13201 atttttaacc ggtagccagg ccgcgtgtaa agcagcctgt aacgccttta ccgatgcagt
    13261 gctggaaatc gcgcgtaatc caatccagcg tgcgtaacgg aggttgccga tgatcaatgc
    13321 actgggattg ctggaagtgg acggcatggt cgccgcgata gatgcagcgg atgccatgct
    13381 caaagcagct aacgttcgtc tgctcagtca cgaagtgctt gaccctggtc gcttaacgct
    13441 ggtggtggaa ggcgatctgg cggcgtgtcg tgcggcgctg gacgcaggtt gtgctgccgc
    13501 gatgcgtacc gggcgtgtca tcagccgcaa ggagatcggt cggccagacg atgacaccca
    13561 gtggctggtt actggcttta accgccagcc gaagcaaccc gtaagggaac ccgacgcgcc
    13621 agttatcgtc gcggaatctg ctgacgagtt gttggcgctg ttaacatcag tacgtcaggg
    13681 aatgacggca ggagaagtgg ctgcccactt tggctggccg ctggaaaaag ccagaaatgc
    13741 gctcgaacag ctcttttctg ccgggacgtt acgtaaacgc agtagtcgtt atcgtctcaa
    13801 gccccattaa cctgtcggag gtgccgggtg tcctacaaca cccggcatta acatcatgaa
    13861 aaagacccgt acagccaatt tgcaccatct ttatcatgaa cccttacccg aaaacctgaa
    13921 gctcacgccg aaggtcgaag tggataatgt tcatcaacga cagacaacgg atgtctatga
    13981 acatgcttta acgattaccg cctggcagca gatttacgat cagctgcatc cgggcaagtt
    14041 tcatggtgaa tttacggaaa ttctactcga tgatattcag gtttttcgtg aatacaccgg
    14101 tctggcgctg cgtcagtcgt gcctggtctg gccgaactcg ttctggtttg gcattccggc
    14161 gacgcgcggt gagcagggat ttatcggttc gcaatgtctg ggaagcgcgg aaatcgccac
    14221 ccgccctggt ggcactgaat ttgaactgag cacgccggat gattacacga tcctgggcgt
    14281 ggtgctttct gaagatgtca tcacccggca ggctaacttt ttgcataacc cggatcgggt
    14341 attacatatg ctgcgtagcc agtcggcgct ggaagtgaaa gagcagcata aagccgcgct
    14401 gtggggcttt gtccaacagg cgctggcgac gttttgcgag aacccggaaa atctccatca
    14461 gcctgcagtg cgaaaagtgc tgggggataa tttgctaatg gcgatggggg cgatgctgga
    14521 agacgcgcaa ccaatggtga cggcggaaag catcagtcat cagagttacc gtagattact
    14581 ttcccgcgcc cgtgaatatg tgctggaaaa tatgtctgag ccggtgacgg tgctggactt
    14641 gtgtaatcaa ctgcatgtca gtcgccgcac gctacaaaac gcgtttcacg ctattttagg
    14701 cattggccca aacgcgtggc tgaaacgcat tcgcctgaac gccgtacgcc gcgaactgat
    14761 aagcccgtgg tcgcaaagca caacggtaaa agacgccgcc atgcagtggg gattctggca
    14821 tctggggcaa tttgccacgg attaccagca gctgtttgcc gagaagccgt cgttgacgtt
    14881 gcatcagcgg atgcgggaat gggggtgagg gagttacacc caatccctga ccttaataaa
    14941 ctcacttaac gccgcttctg ggctgccatc ttttggctga taatcatact cccagcgtac
    15001 cagtggcggc attgacatca ggatcgactc ggtgcgcccg ccagtttgca ggccaaacag
    15061 agtgccgcga tcccagacca gattgaactc gacataacga ccgcgacggt agagctgaaa
    15121 attgcgctcg cgctcgccgt aggccatcgc tttacgtcgc tctacaattg gtaaataagc
    15181 gtcggtgtag cctttgccta ccgcctgcat aaaggcaaaa cagtggtcga aatctggcgt
    15241 gttcagatca tcaaagaaca gcccgccaat accgcgctgt tcgttgcgat gtttgaggta
    15301 gaagtaatcg tcgcaccact ttttgtaacg gggataaacg tcttcaccaa atggcaggca
    15361 caggtcacgg gcggtgcggt gccagtgaat ggcgtcttct tcaaaaccat agaaaggggt
    15421 taaatcgaag ccgccgccaa accaccagac gggctctgca cccggttttt cggcaataaa
    15481 aaaacgcaca ttcgcgtggc tggtgggaac atacgggtta tgcggatgca ctaccagtga
    15541 aacgcccatc gcctcgaaac tgcgcccggc aagctccggg cgatgagcgg tggcggaagc
    15601 aggcatcgcc tcgccgtgga cgtgagaaaa gttgacgcct gcctgttcga aaacaccacc
    15661 attacgcaac acccgactac gcccgccgcc gccagcttcg cgctgccaac tatcttcgac
    15721 aaattctgcg ccatcgacgg cggtcagctg ctgacaaatc gtatcctgaa ggttgagcag
    15781 aaactgttta acctggtgtg cgtcgggttt cataacttat cgctttttcg aatgtgcttt
    15841 ctggttgtcg aaccagtgga aataactgat cacgccttca gcaatcgctg tggcgatttt
    15901 ctgacgaaac gccgccgtgc ctaacagccg ctcttcttcc gggttggtga taaacgaggt
    15961 ttccaccagc accgaaggaa ccgacggcga tttcaacacc acaaatgccg cttgttcggt
    16021 gttgcggctg tgcagtttat gcaccggctt aatcttcttc agaatatgcg agccgagcgt
    16081 cagactattt ttaatggtat ctgtttgcac cagatcaaac agcacttgtt gcaataggtg
    16141 atccttgtca gtcgcctttt taccggcaac ttcatcggcg cggttttcgc gttcagacag
    16201 gtatttcgcc attgcgctac ttgccccacg gttagagagg gcaaataccg aagcaccggc
    16261 agctttcggg ttggtaaagc catcggcatg aattgacata aacagatctg cgccatgttt
    16321 atgggcgatt tcaacgcgat cgtaaagtgg gataaacgta tcgccagaac gcgttaaacg
    16381 cgcatcaatc ccatgattac gcaaaatgga acggacgttt ttagcaatcg ccagcaccac
    16441 atgtttttct ttcgaaccgt tgcgtccgat cgctccggta tcaattccgc cgtgacctgg
    16501 atcgagaaca acgacacgtt tgccgccaga ttttttggct ttcggcttgc tgtgtccgtt
    16561 gctggttttt aaaagttcgt ctttggcgat ggcttgcgac attcctgaca acgtcagggc
    16621 agccaaaccg gctttcagca cctggcggcg cgaagtgagt gtttttagtg gtttaaaagt
    16681 gctcatacgg cctgagttgt aataataaag ttccagatgt tatatcgtat cgcgtaatcc
    16741 gttacgacgg tttgatgttg agaattgttt tacttttcat ttcaatagtt gacaactccc
    16801 cattatgcca ctttttcaac cgattttcgt cagatattgg ctaattcgac cgttcacgcc
    16861 ataatcagcc ttttaacccg caaaaaggta acgaatacca tggagatacg cgtatttcgc
    16921 caggaagatt tcgaagaggt catcaccctt tgggagcgtt gcgacttgct gcgtccgtgg
    16981 aacgatccgg aaatggacat cgagcgtaag atgaaccatg acgtcagttt gtttttggtc
    17041 gctgaggtaa acggtgaagt ggtcggaacg gtgatgggcg gttatgacgg gcatcgcggg
    17101 tctgcttatt atcttggcgt gcatccagag tttcgtgggc gtgggattgc caatgcgttg
    17161 cttaatcggc tggagaaaaa gctgattgct cgtggctgcc cgaaaattca gatcaacgtg
    17221 ccggaagata acgacatggt gctcggaatg tatgaacgcc tgggatatga acacgccgac
    17281 gtgctgagtc tgggtaagcg tttgattgaa gatgaagagt actgagtttc atcctgtcca
    17341 ttatgatgcg cacggtcgcc tgcgtttacc cttgctcttc tggcttgtgc tattgcttca
    17401 ggcgcgaacc tgggtgctgt ttgtcattgc cggtgcgtcg cgcgagcagg gtactgcgct
    17461 gctaaatctg ttttatcccg atcacgataa tttctggctg gggctgattc ctggcattcc
    17521 ggcggtgctg gcgtttttgt tgagcggtcg gcgggctacg tttcctcgca cctggcgtgt
    17581 actctatttt ctgttgctgt tggcgcaggt ggttttactt tgctggcaac cgtggctgtg
    17641 gctgaacggt gaatccgtta gcggtatcgg tctggcgctg gtagtggcgg atattgtggc
    17701 gttaatctgg ctgctgacca atcgacgttt acgcgcttgt tttaatgaag aaaaagaata
    17761 acggcacttt ttggtgaatt tgcactccaa gcaacgttat tgaataacca aaggaagtga
    17821 catgaaatcg ctgcgtttaa tgttatgcgc tatgccgttg atgctgaccg gctgttccac
    17881 gatgtcgtca gttaactggt cggccgctaa cccgtggaac tggtttgggt cgtccaccaa
    17941 agtgagcgag cagggcgttg gtgaattaac ggcgtccaca ccactgcaag aacaagccat
    18001 tgccgatgcg cttgatggcg attatcgcct gcgcagcgga atgaaaaccg cgaacggcaa
    18061 cgtggtgcgc ttttttgaag tgatgaaggg cgacaacgtg gcgatggtga ttaacggcga
    18121 tcaaggcacg atcagtcgca ttgacgtgct ggatagcgat attcctgctg acaccggtgt
    18181 taaaatcggt acaccgttta gcgaccttta cagcaaagca tttggcaatt gccaaaaagc
    18241 tgatggtgat gataatcgtg ccgtcgaatg taaagccgaa ggcagtcaac atattagcta
    18301 ccagttcagc ggggaatgga gtggtcctga agggttaatg ccttcggacg ataccctgaa
    18361 aaactggaaa gtcagtaaaa ttatctggcg gcgttaattt gcgtctgaac aaaccgccgc
    18421 cgcgaaaaaa cacgtaaaat agcgctcaac aatgccacgg attgcgtggc attattattt
    18481 tcaggaggaa caatgtctca ggttcagagt ggcattttgc cagaacattg ccgcgcggcg
    18541 atttggatcg aagccaacgt gaaaggggaa gttgacgccc tgcgtgcggc cagtaaaaca
    18601 tttgccgaca aactggcaac ttttgaagcg aaattcccgg acgcgcatct tggtgcggtg
    18661 gttgcctttg gtaacaacac ctggcgcgct ctgagcggcg gcgttggggc agaagagctg
    18721 aaagattttc cgggctacgg taaaggcctt gcgccgacca cccagttcga tgtgttgatc
    18781 cacattcttt ctctgcgtca cgacgtaaac ttctctgtcg cccaggcggc gatggaagcc
    18841 ttcggtgact gcattgaagt gaaagaagag atccacggct tccgttgggt tgaagagcgt
    18901 gacctgagcg gctttgttga cggtacggaa aacccggcgg gtgaagagac gcgtcgcgaa
    18961 gtggcggtta tcaaagacgg cgtggatgcg ggcggcagct atgtgtttgt gcagcgttgg
    19021 gaacacaacc tgaagcagct caaccggatg agcgttcacg atcaggagat gatgatcggg
    19081 cgcaccaaag aggccaacga agagatcgac ggcgacgaac gtccggaaac ctctcacctc
    19141 acccgcgttg atctgaaaga agatggcaaa gggctgaaga ttgttcgcca gagcctgccg
    19201 tacggcactg ccagtggcac tcacggtctg tacttctgcg cctactgcgc gcgtctgcat
    19261 aacattgagc agcaactgct gagcatgttt ggcgataccg atggtaagcg tgatgcgatg
    19321 ttgcgtttca ccaaaccggt aaccggcggc tattatttcg caccgtcgct ggacaagttg
    19381 atggcgctgt aagcgtttct gattcattga ttaaaggcca ggcagtaaca ttactgactg
    19441 gcctttttat tacttttgct ttaacgccgc atacacctga tcaactaccc aaccatacgt
    19501 tgcaatcggc agctgaccgc tttcgaacat attgggattc ttttgcggat cggcaaccgg
    19561 cgaatgtggc ttgttgctta acatcacaat cgccatatga ttcaccggat cgataacggt
    19621 caccgttccg gtccagccag tgtgaccgta ggtttgcggg cttgccagcg tgccaaacgt
    19681 cggcgtcatg gtggcattac cattcacgcg ccagccgagg ccaaaagtgg catcttcctt
    19741 agagctggtg gtgaacatct tcaccgtttc cgcactgaac agctgcacat cgccatagcc
    19801 cccgccgttc agcatcgttt gcattaacac cgcaatatcg ccggtattgg aaaacaaacc
    19861 tgcgtgcccg gaaacgccgc ccatcgaata aaaggctttt tcatcgtgca cctgacccca
    19921 gagagtggag gtgcggatat tcgggaaatg gatcacgcca tcgcgggtat tgccgttcaa
    19981 ttccgtggcg gcaatttgct gcggtttaaa gcctttcagc agcgggttaa ataccgtatg
    20041 tgtcaggccg agcgggcgat aaatcgactc ttcaacatag cggtcaagcg gctgaccggt
    20101 gaccgactcg acgataaatc caagcagcat ataatcgaca tcactgtaaa tatgcttgct
    20161 gccgggctga tactccagcg gcgtgcgctt gatcatctcc agcgtctggc ctttatcctg
    20221 ggaatataac gcgcccgcga ccgctttatt cggatattgt gggtccgccg ggaaaccgcc
    20281 gctgtgatgc agcaggtcag aaatccgcag cgtgtttttg cctttgatgg tgtcatttgg
    20341 actatcggca aaccccggaa tatatttcgc aatccgatcg tcaggatgca gtttgccttc
    20401 gctcatcagc ttttgcaggg cgaagttcgt ggcgtacatt ttggtgtttg aggccaggtc
    20461 atacagcgtc ccggtggtgg ctttgacagg ctgttccatc aacacgctgc catcgtactt
    20521 tttcgccgct ccccaggctt tgcgatacac aatctgatta tctttaatga tcagcaggtt
    20581 tacgccggga taaccggcgt ccacttgctg gctaatccag cgatccatct ggttaagccg
    20641 ttcgacgttg aaccctgctt tctctggcga actttctgtc agaacagggt attttgcggc
    20701 gctgacgcta caactaacag ccagcagaga aagatagagc attgtccgtt tcatgatatc
    20761 agtccagatt gacgttacgg cagccaaaga gcgtggtgaa aataaacccg caaacccatg
    20821 ccaccagaat cccgccagca taaaccgcca ttgccggaag gatgccttgt gcggaagtca
    20881 tcagcggcag cgccaccaga ccagacggcc caaaggcgct gtttaagccc atcggcagac
    20941 cccaccaggc tatcaggccg ataaacaaac cgcccgctgc gccgcctaaa caggcggtaa
    21001 taaacggttt catgcggggc agggtgacgc cgtaaatcaa cggttcacca acgcccagca
    21061 gaccgggaat aatcgccccg cgtacctgac tgcgtaatgc actgtgcggt tgcgcccgcc
    21121 agtagagtgc cagcgccgcg cccacctggc ccgcgcctgc cattgaaagg atgggaaata
    21181 agctgttgaa tccctggctg tccattaacg cgaggtaaac aggaataaag ccctgatgca
    21241 cgccaaatac cacggcgatc aggaacagcc cggctaaaac cgcacaaccg aacggattac
    21301 tgttcaggtg cataaacagc catgacatac cttcgaataa ccagccaccc agcggcataa
    21361 tgatcaggta cgcaagcgta gcggtgatca gcagggtgat taacgaggtc agcagcatgt
    21421 cgagatcgtc cggcataaag cggcgcacca tgccttcaat gcgggcgcag gcccacgcgg
    21481 caatcagcac gccgataata ttgccgcgcg gatcgatggg cagaccaaag aaatcgtgaa
    21541 aaccggcgta gtaaccggtg gttgcagcgg ggttataacc gagcaaaaag agtgcggcaa
    21601 taattgcgcc attgacgccc gtgccgccaa atgcctgggc ggcgttatag cccaccagaa
    21661 tcaccaggaa agtgaacaaa cctttgctga acaccttcat aaaattcagc gcgtcgggta
    21721 gcgtgccctg agcatctgcc ggaacgtgca tcaccgtggc aattaacgtc gctatcccca
    21781 gcagcagacc ggcggcaata aaaccgggga tcagcggcgt gaagatggtg gcgaatttgg
    21841 cgagaaattg ttgcacgccg gaggtttgtt tggcttttaa ctgacgtttg ttctgggcgg
    21901 cgatttctgc ggcatcctgt accggtgcct cgcccagcag ctcgctcatc gcttttgcgg
    21961 cacgatgcgc tttaccaggc ccaaaaacga cctgcacctg gtcgctggtc aaaatgacgc
    22021 ccttcacgcc ttccagcgtt ttgatattgg gatcaaccag tgaactgtca tgtacaccca
    22081 gacgcaggcg cgtcatacag ttaccacaac tggcgatatt tcccggtccg ccgacacggg
    22141 taagaatggt gttcagaagt tcactgctga tctctttggc catgggttat tccttgtcta
    22201 aaacctgacg aataaagccg ccgtgttgat ccaggcgttt tttagcttct gcggcatcga
    22261 gatttttcag caccatcaca atggccgttt tacagttgcg ctcgcaggca attaacgccg
    22321 cttccgcttg ttcggcatta catccggtgg cgtttttaac gatattgacc tggcgcacat
    22381 gcagtttttc gttggtggcg accacatcga ccatcaggtt gccgaacact ttgccagatt
    22441 taatcatcag cccggtggaa agcatattga gcaccagttt ctgcgctgtg cctgctttca
    22501 ttcgcgaaga accagtgacc acttcggcac ctacaatcgg tgtaatggca aactcggcgg
    22561 tggtggaaac ggcactcccc ggattacagg aaattcccac tgtgcggcag ccgagctggc
    22621 gtgcgtattc cagtccggca atcacatacg gcgtgcgacc gctggcagca atgccaacca
    22681 ccacatcctg tgccgttaaa ttaatatttt tcagatcgtt aacaccacct tcccggctat
    22741 cttcagcgcc ttccaccgcg tgctgaatgg catattcgcc tccagcaatc aaaccaacca
    22801 ccagacccgg tttcacgccg taggtgggcg gacattcgct ggcatccaga atccccagac
    22861 gaccggatgt tcccgcaccg agataaatca gacgaccgcc gccgctgacc tgggcgtgga
    22921 taacatcgat cgccgcggcg atatccggca ggacgcgctc aacggcaagc ggtacggttt
    22981 tatcttcatc gttgataatc cggcacattt ccagcgtcga tacgcggtca atttcagccg
    23041 aggcggtgtt cgagccttca gtaatcatct tttctagttg catcggtggt gaccttacta
    23101 tttacgatca aataattgca gatgtatgga atcatatatc cctaattgac agacttaaag
    23161 gaatcattga gtctacactc gcagagtgtg aagagcgtca taagaactta agcggaattt
    23221 atgctgccag cccaggagag tggaagatgt tgtacctgac gaaaataagc aatgcgggga
    23281 gtgaattcac cgaaaacgag caaaaaatcg ctgactttct gcaggccaac gtgagtgagc
    23341 tgcaatccgt ttcttcacgg cagatggcga aacagctcgg cattagccag tcgagcatag
    23401 tgaaatttgc ccaaaaactc ggcgcgcaag gatttactga attgcgtatg gcgttaatag
    23461 gcgaatacag tgccagccgg gagaaaacga atgccacggc gctgcacctg cacagttcga
    23521 ttaccagtga tgattcgctg gaagtgattg cccgcaaatt gaatcgcgaa aaagagctgg
    23581 cgctggagca aacctgtgcg ttgttcgact acgcgcggct gcaaaaaatc attgaggtca
    23641 tcagtaaagc accgtttatc cagataacgg gcctgggcgg atcggcgctg gtggggcgcg
    23701 atctgtcgtt caaactgatg aaaattggct atcgcgtggc ctgcgaagcc gacacgcacg
    23761 tacaagccac tgtttcccag gcgttgaaaa aaggcgatgt acaaatcgct atctcttaca
    23821 gcggcagtaa aaaagagatt gtcttatgcg cagaagcggc acgaaagcag ggggcaacgg
    23881 tgattgccat tacttctctg gctgattctc cgctgcggcg gctggcgcat ttcacgctcg
    23941 ataccgtttc cggtgaaacc gaatggcgta gctcgtcgat gtctacccgc acggcgcaaa
    24001 attctgttac cgatttactg tttgttggtc ttgtgcaact caatgatgtg gagtcattga
    24061 aaatgattca gcgcagcagt gagctaacac aacgcctgaa atgatgcata aagcagcgac
    24121 tggattgaga ttttcctgaa ttagtgagct gatccgcagc aatattttgt ttatcctgta
    24181 ttttcagagg gaatggagtg taacgctctg tattaacaag gagagcatta aaatgggtaa
    24241 actcacgggc aagacagcac tgattacggg cgcattgcag ggaattggcg aaggaattgc
    24301 cagaactttt gcgcgtcatg gcgcaaacct aatcttgctg gatatctccc ctgagatcga
    24361 aaagctggcg gacgaactgt gtggtcgtgg tcatcgctgt acggcggttg tcgccgatgt
    24421 gcgtgacccg gcgtcggtag ccgcagctat caaacgcgcg aaggaaaaag aagggcgcat
    24481 tgatatcctg gtgaataacg caggcgtttg tcgtctgggc agtttcctcg atatgagcga
    24541 tgacgatcgc gatttccata ttgacatcaa tattaaaggc gtatggaacg tcacgaaggc
    24601 ggtgctgccg gagatgattg cccgcaaaga tggtcgcatt gtgatgatgt cttcggtcac
    24661 tggcgatatg gtggccgatc ctggcgaaac ggcgtacgcc ttaacgaaag cggcgattgt
    24721 tggcctgaca aaatcgctgg cggtggagta cgcgcagtct ggtattcgcg ttaacgccat
    24781 ttgcccggga tacgtgcgca caccaatggc ggaaagcatt gcccgccagt cgaacccgga
    24841 agatccagag tcggtgctga ctgaaatggc gaaagcaatc ccgatgcgtc gcctcgccga
    24901 tccgctggaa gtcggcgaac tggcggcctt cctcgcatcg gatgaatcca gctatttaac
    24961 cggtacacag aatgtgattg atggcggcag cacactgccg gagacggtta gcgtcggtat
    25021 ctgattcacc tctgtttcct ccctgcattt gtggggagga tttcgtttta aacccggttc
    25081 ttattccctt ttcaacttcc aaatcaccaa acggtatata aaaccgttac tcctttcacg
    25141 tccgttataa atatgatggc tattagaaag tcattaaatt tataagggtg cgcaatggcc
    25201 gttaacttac tgaaaaagaa ctcactcgcg ctggtcgctt ctctgctgct ggcgggccat
    25261 gtacaggcaa cggaactgct gaacagttct tatgacgtct cccgcgagct gtttgccgcc
    25321 ctgaatccgc cgtttgagca acaatgggca aaagataacg gcggcgacaa actgacgata
    25381 aaacaatctc atgccgggtc atcaaaacag gcgctggcga ttttacaggg cttaaaagcc
    25441 gacgttgtca cttataacca ggtgaccgac gtacaaatcc tgcacgataa aggcaagctg
    25501 atcccggccg actggcagtc gcgcctgccg aataatagct cgccgttcta ctccaccatg
    25561 ggcttcctgg tgcgtaaggg taacccgaag aatatccacg attggaacga cctggtgcgc
    25621 tccgacgtga agctgatttt cccgaacccg aaaacgtcgg gtaacgcgcg ttatacctat
    25681 ctggcggcat ggggcgcagc ggataaagct gacggtggcg ataaagccaa aaccgaacag
    25741 tttatgaccc agttcctgaa aaacgttgaa gtgttcgata ccggcggtcg tggcgcaacc
    25801 accacttttg ccgagcgtgg cctgggcgat gtgctgatta gcttcgaatc ggaagtgaac
    25861 aacatccgta aacagtatga agcgcagggc tttgaagtgg tgattccgaa aaccaacatt
    25921 ctggcggaat tcccggtggc gtgggttgat aaaaacgtgc aggccaacgg tacggaaaaa
    25981 gccgccaaag cctatctgaa ctggctctat agcccgcagg cgcaaaccat catcaccgac
    26041 tattactacc gcgtgaataa cccggaggtg atggacaaac tgaaagacaa attcccgcag
    26101 accgagctgt tccgcgtgga agacaaattt ggctcctggc cggaagtgat gaaaacccac
    26161 ttcaccagcg gcggcgagtt agacaagctg ttagcggcgg ggcgtaactg atgtttgctg
    26221 tctcctccag acgcgtgctg ccgggcttta ccttaagcct cggcaccagt ctgctgtttg
    26281 tgtgcctgat tttgctgctg ccgctctccg cgctggtgat gcaactggcc cagatgagct
    26341 gggcgcagta ctgggaggtg atcaccaacc cgcaggtggt cgcggcctac aaagtaacgc
    26401 tgctgtcggc gtttgtggca tcgattttta acggcgtttt cggtctgctg atggcgtgga
    26461 tcctaacccg ctatcgcttc ccaggccgca cgctgcttga tgcgctgatg gatttaccct
    26521 ttgcgctgcc aacggctgtc gccggtttaa cgctggcctc gctcttttcc gtaaacggtt
    26581 tttacggtga atggctggcg aagtttgata tcaaagtcac ctatacatgg ctggggattg
    26641 cggtggcgat ggcctttacc agcattccgt ttgtggtgcg taccgtgcag ccggtgctgg
    26701 aagagttagg cccggaatat gaagaagcgg cggaaacgct tggtgcaacg cgctggcaga
    26761 gtttctgcaa agtggtgctg ccggagcttt ctccggcgct ggtggcgggc gtggcgctgt
    26821 cgtttacccg tagtcttggt gaatttggcg cggtgatttt tatcgccgga aatatcgcgt
    26881 ggaagacgga agtgacgtcg ctgatgattt ttgtgcgctt acaggagttt gattacccgg
    26941 cagcgagcgc gattgcttcg gtgatcctcg cggcatctct gctgctgctg ttctcaatta
    27001 acactctgca aagtcgcttt ggtcggcgtg tggtaggtca ttaatggcgg aagttaccca
    27061 attgaagcgt tatgacgcgc gcccgattaa ctggggcaaa tggtttctga ttggcatcgg
    27121 gatgctggta tcggcgttca tcctgctggt gccgatgatt tatatcttcg tacaggcgtt
    27181 cagcaaaggg ctgatgccgg ttttacagaa tctggccgat ccggacatgc tacacgccat
    27241 ctggctgacg gtgatgatcg cgctgattgc cgtaccggta aacctggtgt tcggcattct
    27301 gctggcctgg ctggtgacgc gctttaactt ccctggacgc cagttactgc tgacgctact
    27361 ggacattccg tttgccgtat cgccggtggt tgccggtctg gtgtatctgc tgttctacgg
    27421 ctctaacggc ccgctgggcg gttggctgga tgaacataat ctgcaaatta tgttctcctg
    27481 gccgggaatg gtgctggtca ccatcttcgt gacgtgtccg tttgtggtgc gcgaactggt
    27541 gccggtgatg ttaagccagg gcagtcagga agacgaggcg gcgattttgc ttggcgcgtc
    27601 tggctggcag atgttccgtc gtgtcacatt gccgaacatc cgctgggcgc tgctttatgg
    27661 cgtagtgctg accaacgccc gcgcgattgg cgagtttggc gcggtgtcgg tggtttccgg
    27721 ctcgattcgc ggcgaaacac tttcgctgcc gttacagatt gaattgctgg agcaggacta
    27781 caacaccgtc ggctccttta ctgccgccgc gctgttgacg ctgatggcga ttatcaccct
    27841 gtttttaaag agtatgttgc agtggcgcct ggagaatcag gaaaaacgcg cgcagcagga
    27901 ggaacatcat gagcattgag attgccaata ttaagaagtc gtttggtcgc acccaggtgc
    27961 tgaacgatat ctcactggat attccttcag gtcagatggt cgcgttgctg gggccgtccg
    28021 gttccgggaa aaccacgctg ctgcgcatta tcgccgggct ggagcatcaa accagcgggc
    28081 atattcgctt ccacggcacc gacgtcagcc gcctgcacgc gcgcgatcgc aaggtcggtt
    28141 tcgtgttcca gcattacgcg ctgttccgtc atatgacggt gttcgacaat atcgcttttg
    28201 gcctgacggt gctgccacgt cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga
    28261 caaaattgct ggagatggtg cagcttgctc atctggcaga tcgttatccg gcacagcttt
    28321 ccggcggcca gaaacagcgc gtggcgctgg cgcgcgcgct ggctgtagaa ccgcaaattc
    28381 tgctgcttga tgaaccgttt ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct
    28441 ggctgcgtca actgcatgaa gagctgaaat tcaccagcgt gttcgtgacc cacgaccagg
    28501 aagaggcgac cgaagtagcc gatcgtgtag tggtgatgag tcaggggaat attgaacagg
    28561 ctgatgcgcc gaatcaggta tggcgcgaac cagcgacccg ttttgtactc gaatttatgg
    28621 gcgaagtgaa ccgcctgcag ggaaccattc gcggcgggca gttccatgtt ggcgcgcatc
    28681 gctggccgct gggctacaca cctgcgtatc agggtccggt ggatctcttc ctgcgcccgt
    28741 gggaagtgga tatcagccgc cgtaccagtc tcgattcgcc gctgccggta caggtactgg
    28801 aagccagccc gaaaggtcac tacacccaat tagtggtgca gccgctgggg tggtacaacg
    28861 aaccgctgac ggtcgtgatg catggcgacg atgccccgca gcgtggcgag cgtttattcg
    28921 ttggtctgca acatgcgcgg ctgtataacg gcgacgagcg tatcgaaacc cgagatgagg
    28981 aacttgctct cgcacaaagc gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct
    29041 tcccgcgatc cgggcttttt aatggcaagg tttgtaacct gtagacctga taagacgagc
    29101 aagcgtcgca tcaggcaaca ccacgtatgg atagagatcg tgagtacatt agaacaaaca
    29161 ataggcaata cgcctctggt gaagttgcag cgaatggggc cggataacgg cagtgaagtg
    29221 tggttaaaac tggaaggcaa taacccggca ggttcggtga aagatcgtgc ggcactttcg
    29281 atgatcgtcg aggcggaaaa gcgcggggaa attaaaccgg gtgatgtctt aatcgaagcc
    29341 accagtggta acaccggcat tgcgctggca atgattgccg cgctgaaagg ctatcgcatg
    29401 aaattgctga tgcccgacaa catgagccag gaacgccgtg cggcgatgcg tgcttatggt
    29461 gcggaactga ttcttgtcac caaagagcag ggcatggaag gtgcgcgcga tctggcgctg
    29521 gagatggcga atcgtggcga aggaaagctg ctcgatcagt ttaataatcc cgataaccct
    29581 tacgcccatt acaccactac cgggccggaa atctggcagc aaaccggcgg gcgcattacc
    29641 cattttgtct ccagcatggg aacaaccggc actatcaccg gcgtttcacg ctttatgcgc
    29701 gaacaatcca aaccagtaac gattgttggc ctgcagccgg aagaaggcag cagtattccc
    29761 ggcattcgcc gctggcctgc ggaatatctg ccggggattt tcaacgcttc tctggtggat
    29821 gaggtgctgg atattcatca gcgcgatgca gaaaacacca tgcgcgaact ggcagtgcgg
    29881 gaaggaatat tctgtggcgt cagctccggc ggcgcggttg ccggagcact gcgggtggca
    29941 aaagctaacc ctggtgcggt ggtggtagcg atcatctgcg atcgtggcga tcgctacctt
    30001 tccaccgggg tgtttgggga agagcatttt agccaggggg cggggattta agattattgc
    30061 gcaggcaccg ttttatccgt ctgcgcaatt agcgcatgta aaaaaggctg tgcgttctca
    30121 tcgctcttac cggggatctt cacaatatac ggcttcccgg taatcgatga cgacgaagca
    30181 accttatcaa taaactgctc ggcagtgtca atgcggttac gggtattgcc cagcttcaga
    30241 cgcagatgag aaaccgcttc atcgcaggta tgttcatcac cgttgcgcac aaatatcaaa
    30301 tcctttttct gtgctaatcc ctccagcatg gcgttgatgc gggcttcttc atgggcagtt
    30361 aacttcgcgt aagcggggag tgtcattagt agtgtaatga ccagacaaat gattttcttc
    30421 attgaattac cttatccttg ttttgtgata gccgatttga ctgccagaat caggtaaagt
    30481 ttcggttgga atacgatgag gatcatcggc aaggagaaaa aattaaatga gtagtttgtt
    30541 gttgtttaac gataagagca gggcgctgca ggcggatatc gtcgccgtgc agtcgcaggt
    30601 ggtttacggc agcgtgggca acagcattgc cgtgcctgct atcaaacaga acggtctgaa
    30661 tgtttttgcc gtgccgacgg tattgctgag caatacgccg cattatgaca ctttctacgg
    30721 tggtgcgatt ccggatgaat ggtttagcgg ttatttgcgt gcgcttcagg agcgtgatgc
    30781 gctgcgccag ctgcgcgctg taaccacggg ctatatggga acggcatcgc aaatcaaaat
    30841 ccttgccgag tggctgactg cgctacgcaa agaccatcct gacctattga tcatggtcga
    30901 tccggtgatt ggcgatattg atagcggaat ttatgtcaaa cctgaccttc ccgaggcgta
    30961 tcgacaatat ttactgccgc tggcgcaggg aattaccccc aatatctttg agctggaaat
    31021 cctgaccggt aaaaattgcc gcgatctcga cagtgccatt gctgccgcaa aaagtctgct
    31081 ttcagacaca ttaaaatggg tggtgattac cagcgcctcc ggtaatgaag aaaatcagga
    31141 gatgcaggtg gtggtggtca gtgccgacag cgtgaatgtc atttcccatt cacgggtaaa
    31201 aaccgacctg aaagggactg gcgacctgtt ttgtgctcag cttatcagtg gtttgctgaa
    31261 agggaaggcg ttaaacgatg cggtgcaccg tgcggggttg cgcgtactgg aagtgatgcg
    31321 ctacacccag cagcatgaga gcgatgaatt gattttgccg ccgctggcgg aagcataaaa
    31381 aatggcgccg atgggcgcca tttttcactg cggcaagaat tacttcttga tgcggataac
    31441 cggggtttca cccacggtta cgctaccgga cagtttgatc agttctttga tttcgtccat
    31501 gttggagata acaaccggag tcagggtaga cttggctttc tcttccagta gcggcagatc
    31561 aaattcaatg acagtatcgc caactttcac gcgctgacct tcttcagcaa tacgcttgaa
    31621 gccttcgcct ttcagttcaa cggtgtcgat accgaagtgg acgaacagtt caacgccgct
    31681 atcagattcg atagagaacg cgtgattggt ttcaaagatt ttaccaatgg taccgtctac
    31741 aggcgcgacc attttgttac ccgttggttt gatagcaata ccatcaccaa cgattttttc
    31801 cgcaaaaacg acatccggca cgtcttcgat attgacgatc tcgccagaga gcggagcaat
    31861 gatctcaata gttccggtat ccttcttgtc gtcggaaacc agagatttca gtttatcgaa
    31921 caaacccatg atcttctcct aagcagtaaa ttgggccgca tctcgtggat tagcagattg
    31981 ttttttcttc aatgaacttg ttaaccagcg tcattaactc gtccgttgtc ggttgagcaa
    32041 gagcctgctc tgctaacacc ttcgcatctt cgaagttcgt gttacggata atcttcttaa
    32101 tgcgcgggat agaaatggcg ctcatagaga attcgtccag acccatcccc agcaacagaa
    32161 gtgtagcacg ttcatcgcca gcaagctcac cacacatgcc agtccatttg ccttcagcat
    32221 gagaagcatc aataacttgc ttgatcaagt tcagcacgga cggtgacatt ggttggtaaa
    32281 ggtgtgaaat catatcatta ccacggtcaa ctgccagagt gtactgcgtt aaatcattgg
    32341 tgccgatact aaagaaatca acttctttgg ctaaatgacg tgcaattgtt gcggcagccg
    32401 gtgtttccac cattacgccg atttcaattg actcgtcaaa cgctttacct tcgtcgcgca
    32461 gttcctgttt gtagatttcg atctctttgc gcagtgcacg cacttcttca acagagatga
    32521 tcatcgggaa cataatgcgc aatttaccga aagccgaggc acgcaggata gcgcggagct
    32581 gatcgcgcag gatctcttta cgatccatcg cgatacggat agcgcgccag ccgaggaacg
    32641 ggttctcttc tttcgggaag ttcatgtatg gcagctcttt gtcgccgccg atgtccatgg
    32701 tacgaacgat aaccgcctgc gagccacacg cttcagccac tgctttgtaa gcagcaaact
    32761 gttcttcttc agtgggcagc gcgtcgcggt ccatgaacag gaactcagta cgatacagac
    32821 caacgccttc agcgccgtta cgctctgcac cttcaacgtc acgaaccgta ccaatgttag
    32881 cgcacacttc tacctggtga ccgtccagcg taatagctgg cagatctttc agtttagcaa
    32941 gctctgcttt ttcagaagcc acttgctcct gaacagcgcg cattttatca ataacttcgt
    33001 tggttggatt gacgtaaacc tgattattta cggcatccag aatcagatag tcgtcatttt
    33061 tcacctgaga ggtgacgcta ccggtaccca cgatagcagg cagttccaga gaacgcgcca
    33121 tgatagaggt gtgggaagta cggccacccg cgtcggtgat gaaacccagc accttcttca
    33181 ggttcagctg tgcggtttcg gacggcgtca ggtcagcggc aaccagaatg acttcatcct
    33241 gaatggcgct caggtcgata atcttcaggc ccaggatgtt gcgcagcagg cgcttaccga
    33301 tatcacgtac gtcagccgca cgttctttca ggtattcatc atccagctct tccagggcag
    33361 aagcctgacc ttcgataact tcatgagcag ctgcgtcagc tgtcatgtgc ttatctttaa
    33421 tcagggctat gatttcctgc tccagctcct catcttcgag cagcataata tgcccttcaa
    33481 agatggcttc tttttcttca ccgaacgttt caccagcttt cgttttgatc gtttccagct
    33541 gggctgatgc cttggcacga ccgctcagaa aacgttcaac ttcctgatca acctggtcgg
    33601 cagaaatttt tttccggtca atgacaattt cgtcttcttt cagaagcaga gctttaccga
    33661 aagcgatacc cggggatgct aaaatgcctg aaatcataac cctaccttac ttgtgactga
    33721 tttttaaaag aacccgggaa attactcgag ttccgccatc agtttaacca gatgttcaac
    33781 cgctttctgc tcgtcttcgc cttctgcgga gatagtcaca acggtacctt gagtcaggcc
    33841 cagagtctgc agtttaaaca ggcttttcgc gctggcgctt ttgccgttgg aagtcacagt
    33901 aatttcagaa gtgaagccct tagcttcttt tacaaactgg gcagcagggc gggtgtgcag
    33961 accgttcgga gcggtaatgg taacttcttg ctggaacatt gtatttcccc aacttatagg
    34021 tttagtgttg tggaactaaa gtctagcctg gcggttcgac tttagcctgt attgttagcg
    34081 ccagttttta acagacgcga cgcacgaaga gcgcaataca gtccattgct ggcgggtgtt
    34141 gaccgctgac gtttcgtacg tcattaaaca ttatgccgct aaaggaagaa ttgaaccaaa
    34201 tcataaaatc gattcagctt tggcttttcc tgtaacgatt aatttcgcgc atcaaaataa
    34261 ttagtctggt taaataccag ttccgacagg ctgaatcaat gccagaaggg gcgaaagttt
    34321 gaagcaggcc acaaaaaagc acctaaaaag gtgctttttt acgcattttt aacaagctgg
    34381 cattactgtt gcagttcttt ctcagtgaag agatcggcaa acaatgcggt gcttaaataa
    34441 cgctcacccg atgacggtag aataaccaca atattcttgt tggtaaagct ttcatcttct
    34501 tgtagtttca acgccgcagc aacagctgct ccagaagaga tacctgcaag aataccttct
    34561 tcttccatca gacgacgcgc ggtagaaatc gcttcttcat tggtgatgcc aatgacttta
    34621 tcgaccagct tgagatcgag gttagctggg ataaaaccag cgccaatacc ctgaatttta
    34681 tgcgggccag gtttaatctc ttcaccagcc agtgcctggg cgataactgg agaatcggtt
    34741 ggctcaacgg cgacagagat aagatcggtc ttgcctttgg tgcctttaat gtagcggctg
    34801 acgccagtca gcgtaccgcc agtcccaacg ccagcaataa atacatcaac ctgaccgtcg
    34861 gtatcttccc agatctccgg accggtggtt ttttcgtgaa tttcagggtt tgccggattg
    34921 ctgaattgtt gcagcagcag gtatttctct ggattgctgg cgacaatttc ttctgctttt
    34981 tggattgcgc ctttcatgcc tttagcacct tccgtcagca ccaggtttgc acctaacgct
    35041 ttcagcagct tgcggcgttc aatactcatg gtttctggca tggtcagggt gagtttgtaa
    35101 ccgcgagcgg cagctacata ggccagtgca atcccggtat taccgctggt cggttcaacc
    35161 agttcaacgc ctggtttcag cacgccgcgc ttttcggcat cccaaatcat gttggcaccg
    35221 atacggcact taacgctgaa gctggggtta cgagattcca ccttcgccag aatgcgtccg
    35281 ttaccgatgc gattcaggcg aaccagcggc gtgtgaccga tagtcagcga gttatcttca
    35341 aaaatcttac tcatggcctg tccttaactg tatgaaattg ggatacaaca ggtagcatac
    35401 ccgctcagag aatatgcgga agtaaggatt tagcatatct atatacagaa gggaaataat
    35461 gacatgcaag atggaataag gggcggcata agccacccct gtttcacaca aacggtttac
    35521 aaaataaccg gtagattgtt accgccacat cgcgtgttta tcgcgatagc aatcgaccca
    35581 catcgccgtc gcgccacaaa cggcaacggg catgatgaac agattaagca gcgggatcat
    35641 cgtaaacagg ctggttaaag caccaaactg catattggtg attttgcgtg tgcgcagggc
    35701 ggtgcgcatc tctttaaacg gcactttgtg gttatcgaag gggtaatcgc aatactggat
    35761 ggctaacatc caggcgctaa acaggaacca cagtaccggc gcgacggttt gcccaatacc
    35821 agggattaag taaagaatta gcaatacaat tgcgcgcggc agataccagg caaatttttg
    35881 ccattcgcgt ttcatgattc gcggcacatc tttcatgata ccgaaaatcc cggtatctgg
    35941 cggtgtagcg ccagtcaatc gtgcttccag ttgttcagcc aataaaccgt taaacggagc
    36001 ggcaatccag ttagcaatcg tggagaagaa atagccaaac actaacagca cagagatgac
    36061 agccagaggc cacaacagat aactcagcca ttgtagccag tccggaacgt aactcatgag
    36121 agtcgggatc cagacatcga gctgtgtaaa gagccaccag aatgcgcccc ccatcaacaa
    36181 aatattgacc agcagcggta aaataacgaa acgccgaatc ccaggttgcg agacgagctt
    36241 ccagccttgc gcaaaatagt aaaaaccgct gcgtggggca gatgtgaatg atgaaaccat
    36301 aatcaggatg agctcctttt gaccaatccc aggaaaattc tgcgtatttt accgggtaat
    36361 tgcgcaatgg acagttagga tatgttcgaa aaaacagcaa aaagcacgat ttcatctatc
    36421 tttgtgctgt gaaagttaat agtgcacttg cacttgaggt aatcggcaaa tactcttagt
    36481 gagtaaatgt ttgccgtggt ggcaaggtgt tagaacaaca gagaatataa tgatgcagga
    36541 tttgcgtctg atattaatca ttgttggcgc gatcgccata atcgctttac tggtacatgg
    36601 tttctggacc agccgtaaag aacgatcttc tatgttccgc gatcggccat taaaacgaat
    36661 gaagtcaaaa cgtgacgacg attcttatga cgaggatgtc gaagatgatg agggcgttgg
    36721 tgaggttcgt gttcaccgcg tgaatcatgc cccggctaac gctcaggagc atgaggctgc
    36781 tcgtccgtcg ccgcaacacc agtaccaacc gccttatgcg tctgcgcagc cgcgtcaacc
    36841 ggtccagcag ccgcctgaag cgcaggtacc gccgcaacat gctccgcgtc cagcgcagcc
    36901 ggtgcagcag cctgcctatc agccgcagcc tgaacagccg ttgcagcagc cagtttcgcc
    36961 acaggtcgcg ccagcgccgc agcctgtgca ttcagcaccg caaccggcac aacaggcttt
    37021 ccagcctgca gaacccgtag cggcaccaca gcctgagcct gtagcggaac cggctccagt
    37081 tatggataaa ccgaagcgca aagaagcggt gattatcatg aacgtcgcgg cgcatcacgg
    37141 tagcgagcta aacggtgaac tgcttcttaa cagcattcaa caagcgggct tcatttttgg
    37201 cgatatgaat atttaccatc gtcatcttag cccggatggc agcggcccgg cgttattcag
    37261 cctggcgaat atggtgaaac cgggaacctt tgatcctgaa atgaaggatt tcactactcc
    37321 gggtgtcacc atctttatgc aggtaccgtc ttacggtgac gagctgcaga acttcaagct
    37381 gatgctgcaa tctgcgcagc atattgccga tgaagtgggc ggtgtcgtgc ttgacgatca
    37441 gcgccgtatg atgactccgc agaaattgcg cgagtaccag gacatcatcc gcgaagtcaa
    37501 agacgccaac gcctgataca cttaaggcaa attaactcct cttcgaaccc ccgcttgtcg
    37561 ggggttttta gcattgatgg tgcgatatgg aatcaatcga acaacaactg acagaactgc
    37621 gaacgacgct tcgccatcat gaatatcttt atcatgtgat ggatgcgccg gaaattcccg
    37681 acgctgaata cgacaggctg atgcgcgaat tgcgcgagct ggaaaccaaa catccagaac
    37741 tgattacgcc tgattcgcct acccaacgtg taggcgctgc gccgctggcg gctttcagcc
    37801 agatacgcca tgaagtacca atgctgtcac tggataacgt ttttgatgaa gaaagctttc
    37861 ttgctttcaa caaacgtgtg caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct
    37921 gtgagctgaa gctggatggc cttgccgtca gtattctgta tgaaaatggc gttttagtca
    37981 gtgccgcgac ccgtggcgat ggcaccaccg gggaagatat cacgtctaat gtgcgtacta
    38041 ttcgcgctat tccgctgaag ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg
    38101 gtgaagtgtt cctgccgcag gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg
    38161 gcgggaaagt ttttgctaac ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc
    38221 cgcgtattac agcgaagcga ccgctcactt ttttctgcta tggcgttggt gttctggaag
    38281 gtggcgagct gccggatact catcttggcc gtttactgca atttaaaaag tgggggttgc
    38341 cggtcagcga tcgggtaacg ctttgtgaat cggcggaaga agtgctggcg ttctatcaca
    38401 aagtggaaga agaccgcccg acgctgggct ttgatatcga cggcgtggtg attaaggtca
    38461 actcactgga acagcaggag cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag
    38521 cgtttaaatt cccggcgcag gagcagatga cctttgtgcg tgacgtcgag tttcaggttg
    38581 ggcgtactgg cgcgattacg cctgttgcgc gtctggaacc tgtccatgtt gctggcgtgc
    38641 tggtgagtaa cgcaacctta cacaatgcgg atgaaatcga acgtcttggt ttacgcattg
    38701 gcgataaagt ggtgattcgc cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc
    38761 tttctgaacg cccggaagat acccgtgagg ttgtattccc gacgcattgt ccggtatgtg
    38821 gttctgacgt tgagcgtgtg gaaggtgaag cggttgcccg ctgtaccggt ggcctgattt
    38881 gcggagcgca gcgtaaagag tcgctgaaac actttgtttc ccgccgtgcg atggatgttg
    38941 acggaatggg cgacaaaatc atcgatcagc tggttgaaaa agaatatgtc catactccgg
    39001 cagatctgtt caaactcacc gcaggcaaac tgaccggact ggagcgtatg gggccaaaat
    39061 cggcacaaaa cgtggttaac gcgctggaaa aagcgaaaga aaccaccttt gctcgcttcc
    39121 tctatgcact tggcatccgt gaagtcggcg aggccaccgc agcaggtctg gcggcatatt
    39181 tcggcacgct ggaagcgctg gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg
    39241 ttggcattgt cgttgcatcc cacgttcaca acttctttgc cgaagaaagc aaccgcaatg
    39301 tcatcagcga gctgttggcg gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg
    39361 aagagattga cagcccgttt gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga
    39421 tgtcgcgtga tgacgctaaa gctcgactgg ttgaactggg cgcgaaagtc gcgggcagcg
    39481 tgtcgaagaa aaccgatctg gtgattgccg gagaagcggc aggttctaaa ctggcgaaag
    39541 cgcaggaact gggcattgaa gtcatcgacg aagcggaaat gctgcgtttg ctgggtagct
    39601 gagatggaaa aagagcagct gattgaaata gccaatacga taatgccgtt tggcaaatat
    39661 aaagggcgtc gcttaatcga cctgccggag gagtatctgc tgtggtttgc ccgcaaagat
    39721 gaattcccgg cagggaagct cggtgagcta atgcaaatca cgctgctgat taaaaccgag
    39781 gggctgacgc aactggtcca gcccctgaag cgtccgcttt aagctttatc ggcgctgctt
    39841 tcctgctgtg cctgtaactg ttcggtctgg cgtttgtatc gacgcgccag caccgcacag
    39901 accatcaatt ggatctgatg gaaaatcatc aggggcagca ccatcatacc gatcaccgat
    39961 gtggggaaca gaatgtttgc catcgggatg ccatttgcca gactcttttt cgaaccacaa
    40021 aagacgatag taatttcatc tgccttattg aagcccagtc ggcgtgccat aaagacgtta
    40081 actacaatca cgatagccag aagaacgcag ctgaccacca cgataaacag caatgatccc
    40141 cagccaactt tatgccagat accattaacg acggcttcgc tgaacgctgt ataaaccacc
    40201 aacagaatgg acgtctggtc agttttcgca atccattttt tattgcgcga cacccagtca
    40261 ccaatccacg gccgggaaag atgccccaac acaaacggca gcagcagttg cagcataatt
    40321 ttaccgacct gctcaaggct gccccctgca ccgtgaacat tcatcaccag accaaccagc
    40381 aatggtgaaa ggaaaatccc cagcaggctg gatgctgacg cagaacaaac cgccgccgcg
    40441 acgttaccgc ccgccattga cgtgaaggcg attgcagact gcacggtagc cgggagaatg
    40501 cacaagtaga gaaaaccgga gtagagcatc gggtcgacat ttaccggttt ccaccaggca
    40561 aacagtacac ccagaatcgg aaacagcacg aaggtgctgc acattaccca caaatgcagt
    40621 cgccagtgac caccgccagc aataatcgcc tcacgcgaca acttcgcgcc gtgcataaag
    40681 aacagcaggg caatcgctgc ggtggtcaga ttttcaaaga aggggacgaa atcgcctctg
    40741 gccggaaaga aagaggccag caacaccacc gtgatcaggg ttaaggtgaa aggatcgagg
    40801 atacgaaaaa gtttcataaa gactccagat gatcgatggt tctattgtgc ttcttccagt
    40861 ttgagaaata aaattg
//
LOCUS       DAPPNJ010000013        40276 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000013 DAPPNJ010000000
VERSION     DAPPNJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.013"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(215..1219)
                     /gene="trpS"
                     /locus_tag="RPM73_000523"
     CDS             complement(215..1219)
                     /gene="trpS"
                     /locus_tag="RPM73_000523"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDX6518918.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1212..1970)
                     /gene="gph"
                     /locus_tag="RPM73_000524"
     CDS             complement(1212..1970)
                     /gene="gph"
                     /locus_tag="RPM73_000524"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDX6518919.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPSVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1963..2640)
                     /gene="rpe"
                     /locus_tag="RPM73_000525"
     CDS             complement(1963..2640)
                     /gene="rpe"
                     /locus_tag="RPM73_000525"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDX6518920.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2658..3494)
                     /gene="dam"
                     /locus_tag="RPM73_000526"
     CDS             complement(2658..3494)
                     /gene="dam"
                     /locus_tag="RPM73_000526"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDX6518921.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3601..4887)
                     /gene="damX"
                     /locus_tag="RPM73_000527"
     CDS             complement(3601..4887)
                     /gene="damX"
                     /locus_tag="RPM73_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDX6518922.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KSVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4979..6067)
                     /gene="aroB"
                     /locus_tag="RPM73_000528"
     CDS             complement(4979..6067)
                     /gene="aroB"
                     /locus_tag="RPM73_000528"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDX6518923.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6124..6645)
                     /gene="aroK"
                     /locus_tag="RPM73_000529"
     CDS             complement(6124..6645)
                     /gene="aroK"
                     /locus_tag="RPM73_000529"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDX6518924.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7046..8284)
                     /gene="hofQ"
                     /locus_tag="RPM73_000530"
     CDS             complement(7046..8284)
                     /gene="hofQ"
                     /locus_tag="RPM73_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDX6518925.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8196..8600)
                     /gene="hofP"
                     /locus_tag="RPM73_000531"
     CDS             complement(8196..8600)
                     /gene="hofP"
                     /locus_tag="RPM73_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDX6518926.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8590..9030)
                     /gene="hofO"
                     /locus_tag="RPM73_000532"
     CDS             complement(8590..9030)
                     /gene="hofO"
                     /locus_tag="RPM73_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDX6518927.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9014..9553)
                     /gene="hofN"
                     /locus_tag="RPM73_000533"
     CDS             complement(9014..9553)
                     /gene="hofN"
                     /locus_tag="RPM73_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDX6518928.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9553..10332)
                     /gene="hofM"
                     /locus_tag="RPM73_000534"
     CDS             complement(9553..10332)
                     /gene="hofM"
                     /locus_tag="RPM73_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDX6518929.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10452..13004
                     /gene="mrcA"
                     /locus_tag="RPM73_000535"
     CDS             10452..13004
                     /gene="mrcA"
                     /locus_tag="RPM73_000535"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDX6518930.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTHTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVKIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13172..13732)
                     /gene="nudE"
                     /locus_tag="RPM73_000536"
     CDS             complement(13172..13732)
                     /gene="nudE"
                     /locus_tag="RPM73_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDX6518931.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14052..16187
                     /gene="igaA"
                     /locus_tag="RPM73_000537"
     CDS             14052..16187
                     /gene="igaA"
                     /locus_tag="RPM73_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDX6518932.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16252..16920
                     /gene="yrfG"
                     /locus_tag="RPM73_000538"
     CDS             16252..16920
                     /gene="yrfG"
                     /locus_tag="RPM73_000538"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDX6518933.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16931..17332
                     /gene="hslR"
                     /locus_tag="RPM73_000539"
     CDS             16931..17332
                     /gene="hslR"
                     /locus_tag="RPM73_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDX6518934.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17357..18235
                     /gene="hslO"
                     /locus_tag="RPM73_000540"
     CDS             17357..18235
                     /gene="hslO"
                     /locus_tag="RPM73_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDX6518935.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18370..20094)
                     /locus_tag="RPM73_000541"
     CDS             complement(18370..20094)
                     /locus_tag="RPM73_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDX6518936.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLTVDGE
                     TLEINLSK"
     gene            20473..22095
                     /gene="pckA"
                     /locus_tag="RPM73_000542"
     CDS             20473..22095
                     /gene="pckA"
                     /locus_tag="RPM73_000542"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDX6518937.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22171..23523)
                     /gene="envZ"
                     /locus_tag="RPM73_000543"
     CDS             complement(22171..23523)
                     /gene="envZ"
                     /locus_tag="RPM73_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDX6518938.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23520..24239)
                     /gene="ompR"
                     /locus_tag="RPM73_000544"
     CDS             complement(23520..24239)
                     /gene="ompR"
                     /locus_tag="RPM73_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDX6518939.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24467..24943
                     /gene="greB"
                     /locus_tag="RPM73_000545"
     CDS             24467..24943
                     /gene="greB"
                     /locus_tag="RPM73_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDX6518940.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            25040..27361
                     /locus_tag="RPM73_000546"
     CDS             25040..27361
                     /locus_tag="RPM73_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDX6518941.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMSKKR"
     gene            27799..28026
                     /gene="feoA"
                     /locus_tag="RPM73_000547"
     CDS             27799..28026
                     /gene="feoA"
                     /locus_tag="RPM73_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDX6518942.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28043..30364
                     /gene="feoB"
                     /locus_tag="RPM73_000548"
     CDS             28043..30364
                     /gene="feoB"
                     /locus_tag="RPM73_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDX6518943.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30364..30600
                     /gene="feoC"
                     /locus_tag="RPM73_000549"
     CDS             30364..30600
                     /gene="feoC"
                     /locus_tag="RPM73_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDX6518944.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30803..31681
                     /gene="rpnA"
                     /locus_tag="RPM73_000550"
                     /pseudo
     CDS             30803..31681
                     /gene="rpnA"
                     /locus_tag="RPM73_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            complement(31710..32480)
                     /gene="bioH"
                     /locus_tag="RPM73_000551"
     CDS             complement(31710..32480)
                     /gene="bioH"
                     /locus_tag="RPM73_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDX6518945.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWRGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            32518..33201
                     /gene="gntX"
                     /locus_tag="RPM73_000552"
     CDS             32518..33201
                     /gene="gntX"
                     /locus_tag="RPM73_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDX6518946.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            33260..33835
                     /gene="nfuA"
                     /locus_tag="RPM73_000553"
     CDS             33260..33835
                     /gene="nfuA"
                     /locus_tag="RPM73_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDX6518947.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34196..35512
                     /gene="gntT"
                     /locus_tag="RPM73_000554"
     CDS             34196..35512
                     /gene="gntT"
                     /locus_tag="RPM73_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDX6518948.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(35557..37641)
                     /gene="malQ"
                     /locus_tag="RPM73_000555"
     CDS             complement(35557..37641)
                     /gene="malQ"
                     /locus_tag="RPM73_000555"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDX6518949.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAIRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(37651..40044)
                     /gene="malP"
                     /locus_tag="RPM73_000556"
     CDS             complement(37651..40044)
                     /gene="malP"
                     /locus_tag="RPM73_000556"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDX6518950.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT         9531 a        11002 c        10231 g         9512 t
ORIGIN      
        1 gcaactgtga gatgtggaaa cggcgaggcg cttcacattt tttcggatta ttgcaaatga
       61 ggataaaaaa accgggtttc cccggtttca gagtgatgac gaagtgattt attcgacaat
      121 gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc
      181 aaaggggcga ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg
      241 cttcgtacac cgctttcagc gtacgggaag cgtgtacact ggctttttct gcgccgtctt
      301 tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt
      361 cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc
      421 cttcgaactg tttttccagt tctggaatgc tctggcccgt taccgcagaa aggatatcca
      481 gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg
      541 agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga
      601 taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca
      661 ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca
      721 gcgcgttgaa acgctgggcg atatcgcgac tcagttccag gtgctgtttc tggtcttcac
      781 ccaccggtac cagattagtt tgatacagca ggatgtccgc agccatcagc accggatagt
      841 caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgcgtca
      901 tgcggctcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt
      961 ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat
     1021 acaaggccag cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga
     1081 tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact
     1141 gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa
     1201 aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg
     1261 ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg
     1321 ccgtagttat atccgtaggt taagccaact gatgggcaac ctgccgcttt tgccgcctga
     1381 atatcattgc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca
     1441 gccaccagta acagcgggtc cggatgcggt tttttgtttt gcacatcatc gccgccgata
     1501 accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc
     1561 ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg
     1621 gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgctatagta gcgatcgaac
     1681 agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac ggacggttta
     1741 cccattgttt tacgcagagt cgcacgttcc tgacgcgccc aggccaatgc acgctccatc
     1801 agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggtaac
     1861 tccaacgcat acagcgccat atctaccgca gcagcaagac cgggagcact gtccaccagc
     1921 gtaccgtcga gatcaaaagc gacgccgcga atatcttcaa acttactcat gacttacctt
     1981 tgccagttca ctgcgcattt catcaatgac ttttttatag tctggctggt cgaagattgc
     2041 cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt
     2101 cacgccaccg tccacttcca gacgaatgtc aaagccagac tcgtcgatac ggcgacgtac
     2161 ttcgcgcagt ttatccagtg tttgaggaat gaaagactga ccgccgaaac caggattgac
     2221 ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagatagc tcagaggtgt
     2281 cgccgggtta aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg
     2341 gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa
     2401 atcaggcaca atgcgatcga cgggtttcac catcagatgt acgtcgatag gggcggtaat
     2461 gccatagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg
     2521 gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc
     2581 ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat
     2641 ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc
     2701 agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg
     2761 acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag
     2821 atcagcactg gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt
     2881 tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt
     2941 gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgcgccat gctatcggcg
     3001 taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct
     3061 tccgggaaat agggtttttt gtagcggcca aacggcacat taaactcacc gcgcagattg
     3121 taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg
     3181 aacggatcct ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt
     3241 gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca
     3301 atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg
     3361 gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc
     3421 aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg
     3481 cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga
     3541 cagcatccag cgcttcaggc aactcccgca acgataaccc attgcgggag ataaccttga
     3601 ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctgaac
     3661 atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga
     3721 aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag
     3781 attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag
     3841 agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc
     3901 agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc
     3961 cgtctctttt ggtgctggcg cagaagtcgc agccggtgcc ttcgtgctcg ccacaggagc
     4021 agctggttca gtacgcttcg gcgtctgtgc taccgacttc ggttccgttt tcacggttgc
     4081 ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct ggacgcgtag tggccggacg
     4141 ttcagcagtt tgcgttttcg ccgtttcacg ggatgcattg ccattgcgta ctggcgcgac
     4201 agttgctggt tcagtcggca atgtagaatt gaccgccaca ttgttcaact gttgctgatt
     4261 ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc
     4321 cgttgccgcc ggggtttgcc cttgagtcgg cgtagaagag atcggcggca gagaaacatc
     4381 ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat tggcctgatc
     4441 ggtcgcattg ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaaga
     4501 ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat
     4561 gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt
     4621 acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc
     4681 ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc
     4741 agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc
     4801 agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag
     4861 ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac
     4921 gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt
     4981 acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa
     5041 cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct
     5101 tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg
     5161 gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc
     5221 cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta
     5281 accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca
     5341 gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac
     5401 aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat
     5461 tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg
     5521 ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca
     5581 ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag
     5641 ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac
     5701 gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc
     5761 caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg
     5821 tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt
     5881 taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt
     5941 cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat
     6001 taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc
     6061 tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca
     6121 gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc
     6181 atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa
     6241 cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg
     6301 ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc
     6361 ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat
     6421 accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc
     6481 gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc
     6541 ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc
     6601 ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact
     6661 aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga
     6721 aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg
     6781 gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc
     6841 taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc
     6901 cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat
     6961 cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcata
     7021 aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca
     7081 ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg
     7141 gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc
     7201 cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat
     7261 caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga
     7321 tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca
     7381 ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg
     7441 gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac
     7501 tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaagc
     7561 gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt
     7621 cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc
     7681 atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct
     7741 caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg
     7801 ttttgttatc tcgtagcaaa agacgattgg tgcgtttatc gacggtcata ctccctttgg
     7861 cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta
     7921 tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg
     7981 cgatattgtt attctgccag gcaatggaat gcactgagag aatgttgcct tcctgccgcg
     8041 ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta
     8101 gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt
     8161 cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt
     8221 gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct
     8281 tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggta
     8341 cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg
     8401 ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg
     8461 cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct
     8521 tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac
     8581 aaccagcgtt taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc
     8641 ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc
     8701 cgacggcact gcttcccaca gcgttttcaa cgccaactcg cctccctgcg cggatggatg
     8761 ccaggaaacc agttgcgcgc cggataactg aaaatccagt ggcgaaaagg gcagcgtttt
     8821 ttcctcgctg aagggagtgg tgtctaccag gcgatacagt gcggcccact gctggtgatg
     8881 acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg atagaaaaat
     8941 gagcgtaact aacatcagca accagactgc ccagcaaagc tggcggaggc ggggtgatgt
     9001 ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa
     9061 attgccagcg tccctgcgca tcctgctgcg tggctccccg ctgattgaga tgaaaagacg
     9121 catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtcagcccct
     9181 tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcgtgc tcgggtaaaa
     9241 gcgccgccag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc
     9301 gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa acgtggcttc gttatctgta
     9361 agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc gtctatgcgc gcttcggcgc
     9421 tgcctgtcag acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca
     9481 tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa
     9541 ttggcgggtt catcagtacg ccttcccaag cgccagcccc agcgcgatgg caaagtctcc
     9601 acctggcggc ggtagcggcg gctgacgaac agaaacggcc tcccagggat caaatccgcc
     9661 ttcgccacat atcgcgacgc tttcgggatc aacggataac gctgccgcca gctccttcgc
     9721 gctagtcatc cccaccgcca gtttgcgccc ccagcgacag cgtgtcgccc acagccactg
     9781 ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaattgctg
     9841 taatgcactg gcatccgggg tgatcgcgct cacatgaaca cgcaaccttt ctgccagagt
     9901 aagtagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataggcgg ggctgagtga
     9961 gtcttcgctg taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt
    10021 ccctgacagc caggccgttt gctcccgctc accaagggac atcgacgggc gcggaaatga
    10081 ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc
    10141 gcgactccac ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc gcccatcttt
    10201 gataatgtcg ttctccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgctcc
    10261 ccgcacgatc gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat
    10321 cttaaatgcc attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg
    10381 tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg
    10441 ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc
    10501 tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg
    10561 cgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc
    10621 tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccaccgg
    10681 agatggtgaa agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg
    10741 acccggtggg gatcttccgt gcggcaagtg tggcgctgtt ctccggccac gcgtcacaag
    10801 gggcgagtac cattacccag cagctggcga gaaatttctt cctcagtcca gaacgcacgc
    10861 tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag
    10921 acgagatcct cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg
    10981 gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca actgacgctg aacgaaatgg
    11041 ctgtgatcgc cgggctgccg aaagcgcctt ccacctttaa tccgctctac tcaatggatc
    11101 gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct ggatgaaggg tatatcaccc
    11161 aacaacagtt cgatcagaca cacactgagg cgattaacgc taactatcac gcgccggaga
    11221 ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg
    11281 gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc
    11341 aggccgcaca gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc
    11401 gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac aacaagatta
    11461 ccgatacgct gaaggcgctg ccaacctatg gtccgctgct gcctgccgca gtcaccagcg
    11521 ccaatcctca gcaagcgacg gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg
    11581 aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca gcaaggaccg acgccgcgta
    11641 aagtgaccga tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat
    11701 ggtggctggc acaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg
    11761 gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca
    11821 cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg
    11881 ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttctcgc tgggatgcag
    11941 gtgccggttc tgactggcag ccgaagaact caccaccgca gtatgctggt ccaattcgct
    12001 tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg
    12061 gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcgcaa aacattgtcc
    12121 acaccgaatc gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct
    12181 acgcggtcat ggcgaacggt ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa
    12241 acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata
    12301 ttccagtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag
    12361 atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg
    12421 caaatcaggc gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca
    12481 ctccgctggc attcctgatt aagagtgctt tgaacaccaa tatctttggt gagccaggct
    12541 ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa
    12601 ccggtaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga
    12661 cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg
    12721 gagcgattaa agatcagata tcaggttacg aaggcggtgc caagagcgcc cagcctgcat
    12781 gggacgctta tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgccgc
    12841 caggtattgt gacggtgaaa atcgatcgca gcaccgggca gttagctaat ggtggcaaca
    12901 gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaagcggtg cacgaggtgg
    12961 gtacgaccat tatcgataat ggcgaagcac aggaattgtt ctgattaaaa aggcgcttcg
    13021 gcgccttttt cagtttgctg acaaagtgca ctttgtttat accggatacg gcgtgaacac
    13081 tttacccgtc tagcaacatc tagaaaaatc aatatattgc agaaacttcg taagcctgat
    13141 aagcattatg catcaggcga actttacgca tttacactcg cccctgccct ttcaaccatt
    13201 cacgcacgag gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca
    13261 aatccatcat atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt
    13321 ccagcgattc cgggtagaga tcttgcgcta ccacgatatt cattttgctg gaaaaataag
    13381 acggtgccat gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat ccaacctctt
    13441 cttttagctc gcggttagcg gcttcgtaga cgctttcacc cggatcaatt aaacctttcg
    13501 aaaaacctaa ttcgtaggat tcagttccca ctgcgtattc gcggatcagg atcaggtgat
    13561 cgtccacaat cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa
    13621 cacgccgcac gccattgctg aactccagat ccacgctctc gacggtaaac agtcgggaac
    13681 gggctacagt ttcaacattc agaatggtgg gtttttgtaa tgatttgctc atcgtgggat
    13741 ctatgctgtg aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt
    13801 gcatgttatt tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc
    13861 aataggttga aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga
    13921 tcagcggtta ctgtgatgtg cttaatgatg ctcaagttaa actccacgct tgccgatagc
    13981 caaccgcaga atcatgtatt gtgtctggtg cgactgacca cgcctgacag actaagtaag
    14041 atggggaaag catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac
    14101 ttgcgggatg gctgataaaa gtgcgatcca gacggcgtca gctgccctgg accaacgcct
    14161 tcgcggatgc gcaaacgcgt aaactcacac ctgaagaacg tagcgccgtt gaaaattatc
    14221 ttgagagcct gacgcaggta ttacaggtgc ctggcccaac gggagctagc gcggcaccga
    14281 tctctctggc gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacgc
    14341 gttacggcat ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag
    14401 tccacctgcc ccctttctgg gaacaataca tcaacgatga gaataccgtt gaactgattt
    14461 ataccgattc gctgccgctg gttatttcac tcaacggtca tacgctgcag gagtacatgc
    14521 aggaaacgcg cggctatgcc ttgcaacctg ttccgtccac gcaggcgtcg attcgcgggg
    14581 aagagagtga gcaaatcgag ctactcaata ttcgcaaaga aacgcatgaa gaatatgcgc
    14641 tgagtcgtcc gcgcgggctg cgtgaagcgt tgctgatcgt cgcctccttc ctgatgttct
    14701 ttttctgcct gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcattactgc
    14761 tgctgggcgc aggtctgtgg gggctattcg cgcccccggc aaaatcctcc ctgcgagaaa
    14821 ttcattgtct gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac
    14881 agatcaacaa tatttcactc ggtattatcg acctggtcta tcccgcacac tggcagccat
    14941 acattgctca ggatctcggt caacaaaccg atattgatat ctatctcgac cgccacgtag
    15001 tgcgtcaggg gcgatatctt tcgctgcatg atgaagtaaa aaactttccg ttacagcact
    15061 ggctgcgcag tacgattatc gctgcgggct cgctgctggt gctgtttatg ctgttattct
    15121 ggatcccgct ggatatgccg ctgaaattca ctctctcatg gatgaaaggc gcgcagacca
    15181 ttgaagccac cagcgtaaaa caactggctg acgctggcgt gcgggtaggc gatacattgc
    15241 gtattagcgg tacgggaatg tgtaatattc gcacttccgg aacctggagc gcgaaaacca
    15301 attcaccttt tttaccgttt gactgctcgc agatcatctg gaatgacgcc cgctcattac
    15361 cattaccaga atctgaactg gtcaacaaag cgacggcatt gactgaagca gttaatcgcc
    15421 agctgcaccc taaaccggaa gatgaatctc gcgtcagtgc ctcattacgt tcagcgattc
    15481 aaaaatccgg catggtattg cttgatgatt ttggcgacat tgtactgaag acagcggatt
    15541 tatgttctgc caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca
    15601 gtaaagactg ggacgcgctg gtaaaacgcg ccaacgccgg gaagctcgat ggcgtgaatg
    15661 tgttattacg cccggtgagt gcggaatcgc tggataacct ggtggcaacc tccaccgcgc
    15721 cgtttatcac gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg
    15781 gattcctgat tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat
    15841 cactttacga ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc
    15901 tgatgcatac gccgtttaac gccgaaggta tcgtcacaaa aatcttcact gacgccaatg
    15961 gtacgcagca tattggcctt catccgatcc cggatcgttc cggcctgtgg cgctatctca
    16021 gcaccacatt gctgctactg acgatgctgg gtagcgccat ttacaatggc gtacaggcct
    16081 ggcgtcgtta ccagcgtcat cgcactcgca tgatggagat tcaggcctat tatgaaagct
    16141 gcctgaaccc gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg
    16201 caggttatgc taccctgtcg cgcaaattgc ttcactctgg agatttccct catgcatatc
    16261 aacattgcct ggcaggacgt agataccgtt ctgctggata tggacggcac attgctcgac
    16321 ctcgccttcg ataactattt ctggcaaaag ctggtgcctg aaacatgggg cgcgaaaaac
    16381 ggggttacgc cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat
    16441 acgctaaact ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg
    16501 atgaccaccg agatgggacc gcgtgccgta ctgcgtgaag ataccattcc gtttcttgag
    16561 gcactgaaag ccagcggtaa gcagcgaatt ttgctcacca atgcgcatcc gcacaacctg
    16621 gcggtaaaac ttgagcatac cggtctggac gcacaccttg atttattact ttccacccac
    16681 acatttggtt atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt
    16741 ctgaaagctg aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg
    16801 caatttggta ttcgttactg cctcggcgtg actaatcctg attccgggat tgccgagaaa
    16861 cagtatcaac gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga
    16921 aggagacgcc atgaaagaaa aacctgctgt tgaggttcga ctggataaat ggctatgggc
    16981 tgcccgtttt tataaaaccc gcgcgctggc ccgtgaaatg attgaaggcg gtaaggtgca
    17041 ttacaacggg cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct
    17101 gcgccaggga aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc
    17161 cgccagcgag gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa
    17221 aatggcgctg gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga
    17281 caaaaaagag cgccgcgacc tgttacgatt taaacacggc gacagtgaat aactgtcacc
    17341 tgcaagagag atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaactt
    17401 tgccgtgcgc ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaatca
    17461 cgattatccg cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt
    17521 aaccgctacg ctgaagtttg atggtgatat caccgtacag ctgcaaggcg acggtccgat
    17581 gaatctggcg gttattaacg gtaacaataa ccagcaaatg cgcggtgtgg cgcgcgtgca
    17641 gggcgaaatt ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat
    17701 caccattacc ccgagcgaag gcgaacgcta tcagggcgtg gttggtctgg aaggtgatac
    17761 cctggcggcc tgcctggaag attactttat gcgttctgaa cagctgccga cgcgcctgtt
    17821 tattcgcacc ggcgacgtag acggcaaacc ggctgcaggc ggtatgttgt tgcaggtaat
    17881 gcctgcgcaa aatgcccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat
    17941 caaaaccgaa gaactgctga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga
    18001 agaagaggtg acggtttacg atccgcagga tgtggagttc aaatgcacct gctcgcgtga
    18061 acgttgcgcc gacgcgctga aaacgctgcc tgatgaagaa gttgatagca tcctggcgga
    18121 agatggcgaa attgacatgc attgtgatta ctgcggtaac cactatctgt tcaatgcgat
    18181 ggatattgct gaaatccgca acaacgcgtc tccggcagat ccgcaagttc attaatgagt
    18241 atgtccggca gagagggctc tgccggattt tacgacgtaa tggcctgatg tgctgcgctt
    18301 accaggccta tgaaataaat cgcaaattat tgttggtcag atacagcgtc gtatccgata
    18361 aaagcgttgt cacttactta agtttatttc cagcgtttca ccatctaccg tcagatcgcg
    18421 ccacgcttta ggcttcgtcc ccagttttcc atccttcgca gctgtgagta atttttcttt
    18481 cgttatttcg ttcggaagta aactcatatc aagcgcgtcc cattcttttc tgtctgagtc
    18541 aaagccatag acaatgactc tgtcatcatt aaaagcaaac aggatccgct ccgcctgacc
    18601 atcactattc agatcttgct cgaccaggac gcaggcgtct ttttcaatac aggtcatcac
    18661 gttatagcga tcctggatta aggccgacca gaatgtcgca tccggtttaa cagaaccagg
    18721 ggcaattaac acattatcgg ctaatacttt ttccgatacc tgttgctgaa gatgttgctc
    18781 tccatctaac gccatcagca gatcccgcgc gcgtttcggg tctttcataa acccggcatc
    18841 gcttttcagc gactcaagcg cagcacgtcc atagcgaccg ctctgctcga gcatgtagat
    18901 agttacctgg tctgacgtgt ttttgccgct ctgataacgc gccatatggc tgttcacgct
    18961 aatgcgcata ctgtccagca ccggcgaatt aagaagcacc agtatcacca acaccaataa
    19021 cgaaaccgca aggttcactt tgccctgaag aacaacggga ttttgccctt tacgccagac
    19081 gatgctgaca aaatacccca gcgaccacac cagtaacacc agcaccgcca gcacgccctg
    19141 caagcggtcg acagtccagc cgtactgagc gacccgcagc cataacgccc aggcggcgac
    19201 gaacacatac agcggagcaa ccagcaaagc ggttttaatc aggcaacgca acggccctgt
    19261 ccagggaagt gacgcttttt gcggatcgcg gacaatagcc attaagatca attgcaaaaa
    19321 ggccagcgtt aacagcaacc cggcggcgga gatgtggcga gaaatcgcgc tcaggcccgt
    19381 aaacggcaga gtgatgataa acatcagggt tagcaatgat actaacggca gcaaccccgt
    19441 ggcgattaac gtgaacaact tttgaataga gtcgattaaa cgtgactgtg ttcgcgcgag
    19501 gatcaccgcc agcgcggtaa ccagacctaa cgtgagataa ataaaccagt cggttgcaaa
    19561 aaagagcgta ttgaaaaacg tgatgccaac aagtttaaac aactcactcc acagtaaaag
    19621 caccagccac gtcaagccat tggcgagaaa aatcaccagt aatataagta cgttatgcca
    19681 tactgactgg taaaaatagc gatagcggct actgtcattg cggatgcgaa ggctttgttg
    19741 tatccacggt agcaacaaca ttgccatcaa cagcagatag cagccaaaat cccaaatcgc
    19801 cttttcagct ctccaggggt tcatgccatc agtttgccac ttcaaccagc cgctcatccc
    19861 cagcgtggca ataaacacca gcgccagcca tccccaaagg cgcttctgtt taaaagagat
    19921 cacggagaag agtaaaacgg atgaaaacgc aacggtcgca ggcacgccat aaacaatcca
    19981 actgtgattt tttccggcaa gccaggcgat caacaggtag caaaccagcc cctgaagtaa
    20041 tccggtagca atcatccccc agcgtgttgc cggtgaaagt tcaacgttat ccatgttggt
    20101 tatccagaat caaaaggtgg gttaattatc gcatccgggc agtagtattt tgcttttttc
    20161 agaaaataat caaaaaaagt tagcgtggtg aatcgatact ttaccgattg aatttgcatc
    20221 aatttcatta aggaatgcga ttccactcac aatattcccg ccatataaac caagatttaa
    20281 ccttttgaga acattttcca cacctaaaat gctatttctg cgataatagc aaccgtttcg
    20341 tgacaggaat cacggagttt tttgtcaaat atgaatttct ccagatacgt aaatctatga
    20401 gccttgtcgc ggttaacacc cccaaaaaga ctttactatt caggcaatac atattggcta
    20461 aggagcagtg aaatgcgcgt taacaatggt ttgaccccgc aagaactcga ggcttatggt
    20521 atcagtgacg tacatgatat cgtttacaac ccaagctacg acctgctgta tcaggaagag
    20581 ctcgatccga gcctgacagg ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc
    20641 gtcgataccg ggatcttcac cggtcgttca ccaaaagata agtatatcgt ccgtgacgat
    20701 accactcgcg atactttctg gtgggcagac aaaggcaaag gtaaaaacga caacaaacct
    20761 ctctctccgg aaacctggca gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa
    20821 cgtctgttcg ttgtcgacgc tttctgtggt gcgaacccgg atactcgtct ttccgtccgt
    20881 ttcatcaccg aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc
    20941 gatgaagagc tggcaggttt caaaccagac tttatcgtta tgaacggcgc gaagtgcact
    21001 aacccgcagt ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt taacctgacc
    21061 gagcgcatgc agctgattgg cggcacctgg tacggcggcg aaatgaagaa agggatgttc
    21121 tcgatgatga actacctgct gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac
    21181 gttggtgaga aaggcgatgt tgcggtgttc ttcggccttt ccggtactgg taaaaccacc
    21241 ctttccaccg acccgaaacg tcgcctgatt ggcgatgacg aacacggctg ggacgatgac
    21301 ggtgtgttta acttcgaagg cggctgctac gcgaaaacca tcaagctgtc gaaagaagcg
    21361 gaacctgaaa tctacaacgc tatccgtcgt gatgcgttgc tggaaaacgt caccgtgcgt
    21421 gaagatggca ctatcgactt tgatgatggt tcaaaaaccg agaacacccg cgtttcttat
    21481 ccgatctatc acatcgataa cattgttaag ccggtttcca aagcgggcca cgcgactaag
    21541 gttatcttcc tgactgctga tgctttcggc gtgttgccgc cggtttctcg cctgactgcc
    21601 gatcaaaccc agtatcactt cctctctggc ttcaccgcca aactggccgg tactgagcgt
    21661 ggcatcaccg aaccgacgcc aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg
    21721 cacccgactc agtacgcaga agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct
    21781 tatctggtta acactggctg gaacggcact ggcaaacgta tctcgattaa agatacccgc
    21841 gccattatcg acgccatcct caacggttcg ctggataacg cagaaacttt cactctgccg
    21901 atgtttaacc tggcgatccc aaccgaactg ccgggcgtag acacgaagat tctcgatccg
    21961 cgtaacacct acgcttcccc ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg
    22021 tttatcgaca actttgataa atacaccgac acccctgcgg gtgccgcgct ggtagcggct
    22081 ggtccgaagc tgtaatgatt tgaagctgga gaatatctat ccagtatctt atagaaagca
    22141 aaacgggagg caccttcgcc tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg
    22201 cgttaccggc actggcagcc aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag
    22261 ctccagcatc ccgttatggt tatccacgat acgctgcaca attgccagcc ctaatcccgt
    22321 gccgctaatg gtgcgcgcac tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg
    22381 ttccggcgca attcccggac cgtcatcttc cacctggaac caggcgcgat tcggctccgt
    22441 cccgctgctg actttgatcc agccattgcc gtaacgggcg gcgttgacca ccatattcgc
    22501 caccgcgcgt ttgatcgaca gcgggtgcat tttcacttca atgctgccgg ggtaaagcgc
    22561 ggtttcaatt tcccgctcat agccactttc ggcagcaata acctcaccga gtactgcgtt
    22621 gagatccgcc atttccatcg gcatctcctg tccggtgcgc aggtagtcga taaactgctc
    22681 aatgatggca ttgcactcct cgatatcttt attgatcgat tctgccaggt agccatcctg
    22741 ctcgctcatc atctcggtcg ccaggcgaat acgcgtcagc ggtgtacgca agtcgtgact
    22801 tacccccgcc atcagcagcg tgcggtcatc cgccagttgc ttaacaccag ccgccatatg
    22861 gttaaaggcg cgggtaacgg aacgcacctc ggacgcgcca tactcacgca gcggcggcgg
    22921 aataatccct ttaccgacct gcaaagcagc atgttcgaga tcgaccaacg gtcggttctg
    22981 gatacgaata aacagccacg ccccgcctat cgccaatagc ataatcgcca gcgtatagcg
    23041 gaacagcgga gagaaatcgc cctgatgaat ttcggtcagc ggcacgcgta cccagatatt
    23101 gggcgacagc caggttttca gccagacgac aggcgaactt ttgttgacct caacgcgcac
    23161 ttccgtcggg ccgcccagtt gctgcgccat ctgatggctt aagaattcat agtgttgcgc
    23221 ccaacgcaga cctgcctctt cggcagcctc gttggagtag agagagatcc ccagctcacg
    23281 gtagatctcc cgacggaaag cgggaggcac aaccaactgc gtgccgtcct ccagttgcag
    23341 tttgtcggtc atcaacatac gcacttcgta cgcgaggact ttattaaact gctggaggct
    23401 cggcaaaatc gcgaagttca gcaccaccag ataagtcgtc accaggctgg cgaacagcaa
    23461 ggtgacgatg agcaataacg tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct
    23521 catgctttag agccgtccgg tacaaagacg tagcccagac cccagacggt ctgaatgtaa
    23581 cgcggatgcg ctggatcttc ttccaccatg cggcgcagac gcgaaatctg cacgtcgatg
    23641 gagcgttcca ttgcggaata ttcacgacca cgggcaaggt tcatcagctt atcgcgggag
    23701 agcggctcac gcggatggct gaccagtgcc ttcagtaccg caaactcacc gctggtgagc
    23761 ggcatcggct cgtcttcgcg gaacatttcg cgcgtaccga ggttaagttt gaacttaccg
    23821 aaagcaatta ccgcctcttc ctgtgacggt gcgcctggta gctcgttcgc ctgacgacgc
    23881 agcaccgcac ggatacgggc cagcagttca cgcgggttaa acggttttgg aatgtaatcg
    23941 tcagcgccaa tctccaggcc aacgatacgg tccacttctt cccctttcgc tgtcaccata
    24001 atgatcggca tcgggttgct ctgactacga agacgtcggc aaatcgacaa gccatcttca
    24061 ccaggtaaca ttaaatccag taccataaga tggaaagatt cacgagtcag caggcgatcc
    24121 atctgttctg cattagcgac gcttcgaacc tggaagcctt gttcggtgag ataacgttcc
    24181 agcagcgcac gcaggcgcat gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt
    24241 gtttgtactc ccaaaggttc gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt
    24301 tcgtcaccag ctaaatctgg tatgaatttc agcctaaatt gttacaaagc atattaaaca
    24361 gcagcttaag tatacaattt attcggcgaa acattattga ttctgttgat atgatcacgt
    24421 tatacccaat gtgcgcatta tcaaacagac aaagggaatc aacgagatga aaacgcccct
    24481 ggttacccgg gaagggtatg aaaaactcaa acaagagctt aattatctct ggcgtgaaga
    24541 acgcccggag gtcacaaaaa aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa
    24601 tgctgactat cagtataata aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct
    24661 cactaaatgc ctggaaaatc tcaaaatcgt cgattactcc cctcagcagg aaggcaaagt
    24721 cttttttggc gcgtgggtgg agattgaaaa cgacgatggc gtgactcacc gtttccgtat
    24781 tgtcggctac gatgaaattt ttggccgtaa agattacatc tctatcgatt ccccgatggc
    24841 ccgcgcattg ctgaaaaaag aagtcggcga tctggcggtg gtgaataccc ctgccgggga
    24901 agcgagctgg tatgttaatg ctatcgagta cgtgaaaccg taaggaagag tcttaacctc
    24961 ctgccgatgg ctggcatttt tgccagccag tccgtataac tatcccctga tttttgatcc
    25021 gaaaagatga actcaaacca tgatgaatga ttcgttctgc cgcattattg cgggtgaaat
    25081 tcaggcgcgc ccggaacagg ttgacgctgc cgttcgcctg cttgacgaag ggaataccgt
    25141 gccgtttatc gcacgttatc gtaaggaaat caccggcggt ctggatgaca cgcagctgcg
    25201 taatctggaa acgcgtctga gctatctgcg cgagctggaa gagagacgtc aggcgatcct
    25261 caaatccatt tccgagcaag gcaaactcac cgatgatctg gcgaaggcca tcaacgccac
    25321 cctaagcaaa accgaactcg aagacctcta cctgccctac aaacctaaac gccgcacccg
    25381 cgggcaaatc gccattgaag cagggcttga gccgttggct gacctgctgt ggagcgatcc
    25441 gtcacacacg ccagaagtcg cggctgcaca atatgttgat gccgataaag gcgtggcaga
    25501 taccaaagcc gcgctggacg gcgcgcgcta tatcctgatg gaacgttttg ccgaagatgc
    25561 cgcgctgctg gcgaaagtgc gtgattatct gtggaagaac gcgcatctgg tctcaaccgt
    25621 ggtgagcggt aaagaagagg aaggggcaaa attccgcgac tatttcgatc atcacgaacc
    25681 gttgtccacg gtgccttctc accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt
    25741 actccagctt tcgctgaatg ccgatccgca gttcgatgag ccgcccaaag agagctattg
    25801 cgagcaaatc atcatggatc accttggcct gcgcctgaac aatgccccgg cggatagctg
    25861 gcgcaaaggc gtagtgagct ggacctggcg catcaaggtg ctgatgcatc tggaaaccga
    25921 actgatgggt accgtgcgcg aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa
    25981 cctgcacgat ctgctgatgg cggcccctgc cggactgcgt gcaacgatgg gcctcgatcc
    26041 gggtctgcgt actggggtaa aagtggcggt ggtcgatgcc actggcaaac tggtagcgac
    26101 cgataccatt tacccgcaca ccggacaagc cgcaaaagca gcgatgactg ttgctgcgct
    26161 gtgtgaaaag cataacgttg aactggtggc gatcggcaac ggtacagctt cccgcgaaac
    26221 cgaacgtttc tatctcgacg tgcagaagca gttcccgaaa gtgaccgcac agaaagtgat
    26281 cgtcagcgaa gctggcgcgt cggtttactc agcttccgag ctggcagcgc aggagttccc
    26341 ggatctcgac gtttcgctgc gtggtgcggt atctatcgcc cgccgtttgc aggatccgct
    26401 ggcggagctg gtgaaaatcg atccgaaatc tatcggcgta ggtcagtatc agcatgacgt
    26461 cagccagacg caactggccc gcaaactgga cgcagtagta gaagactgcg taaacgccgt
    26521 tggcgtcgat ctcaacactg cttctgttcc gctgttaacc cgcgtggcgg gcctgacgcg
    26581 catgatggcg caaaacatcg tggcctggcg cgatgagaac ggtcagttcc agaaccgtca
    26641 gcaactgctg aaagtgagcc gtctggggcc gaaagccttc gagcagtgcg cgggcttctt
    26701 gcgcattaac catggtgata acccgctgga cgcgtctacc gtccacccgg aagcctatcc
    26761 ggtggtggaa cgcattctgg cagcaacaca gcaggcactg aaagatctga tgggtaacag
    26821 cagcgaactg cgtaacctga aagcgtctga ctttactgat gaaaaattcg gtgtgccgac
    26881 agtaactgac atcatcaaag agctggaaaa accgggtcgc gatccgcgtc cggaatttaa
    26941 aaccgctcag tttgccgatg gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct
    27001 cgaaggcgct gtcaccaacg tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca
    27061 ggacggcctg gttcacatct cttcattgtc gaacaagttt gtggaagatc cgcataccgt
    27121 ggtgaaagcg ggcgacattg tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg
    27181 tatcgccctg accatgcgtc tggacgagca gcctggcgaa accaacgccc gtcgcggcgg
    27241 cggtaatgaa cgcccgcaaa acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca
    27301 gcctgccggt aatagcgcga tgatggatgc gctggcggcg gcaatgagca aaaaacgtta
    27361 aacgccctga ttgaccccta aacctgcgcg ataaggtgtc cacgccgcat ctggcaacga
    27421 acgccttgtc tgacataaaa gtgccggaga atatctccgg catttttatt ccacagccaa
    27481 actcacaata tattccggca acatttatca cttcattaac aactgaaacc ttaattaaac
    27541 attagccagt cctggtaatt cgctattcga attatatttt cgctgcgata taaccttgag
    27601 ccacatcaac attgagtcag attattattc aaaccaacat tcgcacacat tttaagtatt
    27661 gctgatagaa accattctca ttatcattgt gctgttgatt atttaatctc tccttcgttg
    27721 gcaaatcatc tggtctcatg tcgctgtcaa acgccccatg aggtagttat ccagttaatg
    27781 agaaacaagt aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt
    27841 tcccgtgaaa tcagcccggc atatcgccaa aaactgcttt ctcttggcat gttacctggc
    27901 tcctctttta atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt
    27961 cgtgtgagcc tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc
    28021 tgttaatacg gtgataacaa caatgaaaaa attaaccata ggcttaattg gtaatccaaa
    28081 ttctggcaag acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg
    28141 ggctggcgtt accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac
    28201 gctggtggac ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga
    28261 tgagcaaatc gcctgtcact acattttgag tggcgacgcc gacctgctga ttaacgtggt
    28321 ggatgcgtct aaccttgagc gtaacctgta cctgacgcta caactgctgg aactcggcat
    28381 tccctgcatc gtggcactga acatgctcga cattgccgag aagcaaaata ttcgtattga
    28441 aattgatgct ctgtcggcgc gtcttggctg cccagtgatc ccgctggttt caacccgtgg
    28501 tcgcggtatt gaagcgctca agctggcgat tgatcgctat aaagctaacg agaatgtgga
    28561 actggtgcat tacgcacagc cgctactcaa cgaagcagat tcactggcaa aagtgatgcc
    28621 ttccgacatc ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat
    28681 ctacagccgc gcctacgccg gtgaagcgtc gcagcatctg gatgccgccc tcgcccgtct
    28741 gcgtaatgag atggacgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc
    28801 tgccatctgt gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc
    28861 ggtagataaa atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta
    28921 cctgatgttc ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg
    28981 ctccgtggcg ctatttgtgc atggtattca atggattggc tacacgctcc acttcccgga
    29041 ctggctgact atcttcctcg cccagggcct gggtggcggc attaacaccg tgctgccact
    29101 ggtgccgcag attggcatga tgtacctgtt cctctccttc cttgaggact ccgggtatat
    29161 ggcgcgtgcg gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc cggggaaatc
    29221 ctttgtgccg ctgatcgtcg gtttcggttg taacgtaccg tcggtaatgg gtgcacgtac
    29281 gcttgatgca ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg
    29341 cgcgcgtctg gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgcgct
    29401 ggcggtcttc tcgctgtata tgctgggtat tgtgatggcg gtgctgactg gcctgatgct
    29461 caagtacacc atcatgcgcg gtgaagcgac gccgtttgtc atggagttgc cggtctatca
    29521 tgtaccacac gttaaaagcc tgattatcca gacctggcag cgtctgaaag gcttcgttct
    29581 gcgtgctggt aaagtgatca tcatcgtcag cattttcctg agcgctttca acagcttctc
    29641 gctgagcggg aaaatcgtcg ataacatcaa cgactcggcg ctggcgtccg tcagccgggt
    29701 gatcaccccg gtcttcaagc caattggcgt gcatgaagat aactggcagg caacggttgg
    29761 cctgtttaca ggtgccatgg cgaaagaagt ggtagtgggt acgctcaaca ccctctacac
    29821 cgcagaaaat attcaggacg aagagttcaa tccggcagag tttaacctcg gtgaagagct
    29881 gttcagtgcg atagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact
    29941 gatgaacccc attgaagcca gcaaaggcga cggcgaaatg ggtaccgggg cgatgggcgt
    30001 gatggatcag aaattcggta gcgcagcagc agcttacagc tacctgattt tcgtcctgct
    30061 gtatgtacca tgtatctcgg tgatgggagc catcgcccgt gaatcaagcc gtggctggat
    30121 gggcttctcc atcctgtggg ggctgaatat cgcttactcg ctggcaacat tgttctatca
    30181 ggtcgccagc tacagtcagc acccaactta cagcctggtg tgcattctgg cggttatcct
    30241 gtttaacatc gtggttatcg gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact
    30301 gctggcaacc cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca
    30361 ttaatggctt cacttattca ggtgcgcgat ttgctggcgt tacggggccg tatggaagcg
    30421 gcccagataa gccagacatt gaacactcca cagccaatga ttaacgccat gctgcaacaa
    30481 ctggaaagta tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc
    30541 agttgtaaaa gctgcccgga aggaaaagcc tgtctgcgcg agtggtgggc gctgcgttaa
    30601 ccttactcca tcgcctgttt tggaaagccc ggtatgcgtc tgcatccggg cttttttgcg
    30661 tgcggctttc cataaaaatg caactcttgc agcacggcgt aagttccttt gaaagcatct
    30721 cgcagggatg aaaactcgct aatacacagg tgtggagtgg cgcgtagagt cgcggcattc
    30781 aaacaacagg tgaaggaacg ccatgagcaa aaagcagagt tccaccccac acgatgcgct
    30841 gttcaaactc tttttacgcc aaccggacac ggctcgtgat tttcttgcgt ttcatttacc
    30901 ggcacccatt cacgcgcttt gtgatatgaa aaccctcaag ctggagtcga gcagctttat
    30961 tgatgacgat ctgcgtgaaa gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg
    31021 accaggatac atctattgtc tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt
    31081 tcgcatgatg cgttaagcta ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac
    31141 gttgccgatg gtggtgctat tgttgtttta ccacggtatt gaaagcccct atccctattc
    31201 gctgtgttgg ctggattgtt tcgccgatcc caaactggca aggcagcttt atgcctccgc
    31261 atttccgctg attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat
    31321 ggcgctgctg gagttaattc aaaaacatat tcgtcaacgc gacctgatgg ggctggtaga
    31381 gcaaatggcc tgcttattaa gtagtggata cgctaatgac agacaaatca aagggctgtt
    31441 taattacata ctgcaaaccg gcgacgcggt acgttttaac gattttatcg acggcgttgc
    31501 cgaacgttca ccgaaacaca aggagagttt aatgactatt gcggaaagat tgcggcagga
    31561 gggggaacaa tccaaagccc tgcatatagc caaaataatg cttgaatccg gagtccctct
    31621 tacagacatc atgcgcttta ccggggtgtc agaagaagag ttggctgcgg cgagccagta
    31681 aatttctgtc tcgccatttc aaaagttgcc tacaccctct gcttcaacgc caccagcagg
    31741 tgacaaaact cgaccggatg cgaaataaac ggcgcatggg ccgctttggc gaagatatat
    31801 gattcgctgt gaggccaaag tttatccagc atcggcacca ctttgcgcgg caccagaccg
    31861 tcgagatagc catacaatcg caaaaacggc atggacacgt tttgcagcgg ctgacggaga
    31921 tcgaccgttt tcaggatttc cagcccgcca ttaagcacgt caacctccgg catcggtaac
    31981 gccagaacgg ttttcttcaa cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt
    32041 tgtaacgcca ggaaccgctc cactgtacgc tgaaaatcat cactgagttg ctgctgaaat
    32101 cccgccagca cgtccggttt tatcccccgc cactcgtcac gagcactaaa acaaggtgac
    32161 gacgccacgg tgaccagcgc ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc
    32221 accagcccgc ccagactcca gcctaaccaa atggctttat caggtgcctg ttgcagcacg
    32281 gcttcggcca tatcagcaag tgacagcgca ccaaatcccc ggctacgccc gaagccgggc
    32341 aggtcaacaa ggtgcagcgt aaaatgcgag ctaagttcct cgtcaatgca acgccacact
    32401 tcggcattca gtccccatcc gtgcagcagc acaagatgaa cattcccctg acctttggtc
    32461 tgccaccaga tgttattcat ccgctattgt tctcttttga cttacaagga tgaacatatg
    32521 ctaacagtac cgggattatg ctggctatgc cgaatgccac tggcgttagg tcattggggg
    32581 atttgttcgg tctgttcacg cgccacccgc acagataaaa cgttatgccc acaatgtgga
    32641 ttacccgcca cacactccca tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg
    32701 caaagactgg tcacggttgc cgactatgcg ccgccgttaa gtccgcttat ccaccagctt
    32761 aaattttccc ggcgcagtga aatcgccagc gccctgtcac gtctgttact attggaagtc
    32821 ttacacgctc gtcgcaccac cgggttgcaa ttgccggatc gcatcgtcag cgttccgtta
    32881 tggcagcggc gtcactggcg tcggggattt aatcagagcg atttgctgtg tcagccgtta
    32941 tcacgctggt tgcactgcca atgggatagc gaagccgtca cacgtacacg ggccactgcg
    33001 acccagcatt ttctcagtgc ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt
    33061 gaattgcccg tgcaaggtcg ccatatggtg attgtggatg atgtcgttac caccggaagt
    33121 accgtcgcag agattgcgca gttgctttta cgcaatggtg cggcggctgt ccaggtctgg
    33181 tgcctttgtc gaaccttgta gagcctcgat gatgggcgta ttataaccaa ctaaaatagt
    33241 caactattag gccattacta tgatccgtat ttccgatgct gcacaagcgc actttgccaa
    33301 actgctggca aatcaggaag aagggacaca aatccgcgta tttgtgatta accctggcac
    33361 gcctaacgct gaatgtggcg tttcttattg tccgccggac gctgtggaag ccaccgacac
    33421 agccctgaaa tttgacctgc tgaccgcgta tgttgatgag ttaagcgcac catacctgga
    33481 agatgcagag atcgactttg ttaccgacca gttgggttct cagttaacgc tgaaagcccc
    33541 gaacgccaaa atgcgtaaag tggcagacga tgcaccgctg atggagcgcg tggagtatat
    33601 gctgcagtcg cagatcaacc cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga
    33661 aatcaccgaa gacggttacg ccattctgca atttggcggc ggctgtaacg gttgttccat
    33721 ggtcgatgtg acgctgaaag aagggatcga gaagcagctg ctgaacgaat tcccggagct
    33781 gaaaggtgtg cgcgatctca ccgaacacca gcgcggcgaa cactcctact actaagttat
    33841 cccctctttt gtagattgcc cgatgcgacg ctaaagcgtc atttcgggcc tacgttaaac
    33901 atgtgccaga cgttggcgca gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat
    33961 aatatgacca acctctcata atttaaattt accccgctct ggtgattctc aaacgccaga
    34021 tgttacccgt atcattcaca tgggtaccaa acatactcct gacatctgac tacaataatt
    34081 agttttagtg ggtatcagtc gtggtgccgc aatatctctg ttcccgattg ggataattag
    34141 agtttgtcgt cagaaaattg acgttaccca taacaaatga aaggccaggt aaatcatgcc
    34201 attagtcatt gttgctatcg gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat
    34261 gaacggcttc atcgctctcg tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc
    34321 gctggataaa gttattggct ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc
    34381 cctgatcatg ggttttggcg caatgctggg caaaatgctg gcagactgcg gtggcgcaca
    34441 acgtatcgcc accacgctga ttgccaaatt tggtaaaaag cacatccagt gggcggtggt
    34501 gttaaccggt tttaccgttg gttttgccct gttctatgaa gtgggcttcg tgctgatgct
    34561 gcctctggtg tttaccatcg cggcttccgc gaatattcca ctgctgtatg ttggtgtacc
    34621 aatggcggct gcactgtctg tgacccacgg cttcctgcca ccgcatccgg gcccgactgc
    34681 gattgccacc attttcaatg ccgatatggg taaaaccctg ctgtacggta ctattctggc
    34741 aatcccgacc gtgattctcg ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa
    34801 gccaatcccg gaaggtctct acagcgcgaa aaccttcagc gaagaagaga tgccgagctt
    34861 tggcgtcagc gtctggactt ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc
    34921 cgaaatgatc ctgccgaaag gtcacgcttt cctgccggta gcggagttcc tcggtgaccc
    34981 ggtaatggca acgctgattg ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg
    35041 tcgttcaatg gatcagatta acgacacgct ggtttcttcc atcaaaatca ttgcgatgat
    35101 gctgttgatc atcggtggtg gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga
    35161 caaatacatt gcttccatga tgcacgaaac caacatttct ccgctgctga tggcctggtc
    35221 gattgctgcc gtactgcgta tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg
    35281 tggtatcgcg gcaccgctga ttgcaacgac gggtgttagc ccagagctga tggttattgc
    35341 ggtgggttcc ggtagtgtga ttttctctca tgtgaacgat ccgggcttct ggctgttcaa
    35401 agagtacttt aacctgacta tcggcgagac catcaaatcc tggtcgatgc tggaaaccat
    35461 tatctcggtg tgcggtctgg taggctgtct gctgctgaat atggtgattt gataacactg
    35521 actgccggat gcatcgcatc cggcatcaac cgcactctac ttcttcttcg ctgcagctct
    35581 gcgccgtctg tccaaatcct tcagcaactt gttcacgcca tcatcggcaa acatcgactc
    35641 aagcgttgcg gaaagcttgc gtcgccagtt tttatactgg taactggtgc caggaatatt
    35701 caccggttcg gccatatcca gccagtcttc cggctgtagt cctaacagag cactgttact
    35761 gtcggcaatg tagcgctgca aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc
    35821 cttatgcccg gcacgttttg gcagacaacc atatttatgc agtgcatcca gcagcccttg
    35881 cttcgccagt tcgcgatcct gatacagacc gcgcagtacc acttcatccg gatacagccc
    35941 cagggttttg cccaacgtta gatccccgct ctcccagtaa ccgcgcagcg taggcaggtc
    36001 atgtgtcgcc gcaaccgcca tcgactgctc cggatacgct ttcggtgcac ggaacgtctt
    36061 ctcgtggtcg ttttcgaaat agagcacttt gtaggagtac acaccgctgc tgcgcagctt
    36121 accgacaatc tctaccggta cggtaccgag atcttcacca atcaccatac agcgatgacg
    36181 tttactttcg agtgccagaa tcgagagcag atcatccacc ggatagtgaa catacgcgcc
    36241 ctgatctgcc gtctcgccat acggtatcca ccacaaacgc agcatcgaca tcacatggtc
    36301 aattcgtaat gcgccgcagt tttgcatatt ggcacgcaac agctcgataa acggttcata
    36361 ggcacgcgcg gtaatgatat gcgggtccat tggcggtaat ccccagttct gccccaacgg
    36421 gccgaggata tccggcggcg cgccaaccga cgctttcagg caatatagtt cacggtcaca
    36481 ccaggtttcc gccccacctt ccgctacgcc aaccgccaga tcacgataca agccaatcgg
    36541 catttcatag ccctggctta tctcccagca ggcggcaaac tggctgtaag ccagccactg
    36601 caaccagaga taaaaatcga cgtcattacg atgttcttcg cagaactgac gcacttctgg
    36661 tgaatccacg ttctgataca tctctggcca tgcaggccag ccccagcgca tttcgtcctc
    36721 tttcacttgc tgggcatgta gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg
    36781 ctctgcaaca aactggcgaa acgcggccat ctgctcatca tcacgttgcg cgaaaccttt
    36841 ccacgccatt cgtaatgctg tcatttttag ggcggtaacc gtggagtaat cgacccagtc
    36901 ggcatcgcgc gcctgttgca gcgtctgttg cgtggtcggc aactgccacc aggcctgagc
    36961 ctcttcgcta agatggaaat cttcaacggc gttaacgtcg atataaatca cattcagcca
    37021 acggcgagaa gacgggctgt atgggctggc actctccgga tttgccggat agagcgcatg
    37081 aatcgggttc aggccgatga acgacccgcc acgtattgcc acatccacca gcatcgcttt
    37141 gagatcgcca aaatccccaa taccccagtt tttttccgat cgcagcgtat aaagctgaac
    37201 gcaggcaccc cacagctttt gtttattcag caacgcctgc ggttcgtaac agcgtttcgg
    37261 ggcgacaatc acccggcaat gcgcgcgctg gtcgtcctgg gtgagtgtca gcgtgtgata
    37321 accttccggc agcttcgtcg gtagattgaa cgctttgccc cccgttacat ggcctttgta
    37381 ctgtgttcct tcttcggtgg tcagcagcca gctatattcg ccgctgccct ccaccaccat
    37441 cggcattttt ttgccgctgg tataaaccat gacattcggg actggcgtta ccgccacttt
    37501 cgtggcggta cgttgatgca tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga
    37561 ctgcggttta ccgtgggcat tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt
    37621 atccagacgt ttgctttcca tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg
    37681 ataatcgcga atagagcgat ccgagctaaa cataccgcag cgggcggtat tgaggatcgc
    37741 cgcgcgagtc caggcctcct ggtcgcggta cagcacatcc acctgctttt gtgcctcaac
    37801 ataggctgcg aaatccgcca tcaccagata cggatcgccg ccctgtttgc cgatactgtg
    37861 cagcatctgg tcgaaggcat gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt
    37921 caatactgcg tccagcacct tatctttctt ccgccatttc accgggtcgt agcctttggc
    37981 cagaatagcc ttcacttgtt ccacggtatg accaaaaata aagatatttt cttcaccgac
    38041 tttctcggcg atttcaacgt tcgcaccatc cagcgtaccg acagtaagcg caccattgag
    38101 cgccagtttc atattgccgg taccggaagc ttctttacct gcagtcgaaa tttgttcgga
    38161 gatatccgcc gccgggatca gtttttccgc cgccgaaacg caataatccg gcaggaacac
    38221 caccttcaac ttatcgccaa ccagtggatc gttgttgatc acgtcagcca ctttgttgat
    38281 cgcaaagata atattcttcg ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa
    38341 gacgcgcggt acgcgatcag cctgcgggtt ttcacgaatt tctttgtaca acgccagaat
    38401 atgcagcaga ttcaggtgct ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa
    38461 aatcgcctgt ggattgatct caataccggt acgaactttc acaaactccg ccagacggac
    38521 tttattcgcc tgcttgatct cgcgatattg ctgacggaat ttcgcatcat cagcgaattt
    38581 ttccagattg atcagctgat cgagatcgtt agcccactct ttttgcagtg atttatccaa
    38641 cagagccgcc agtgcagggt tgcactgttt gatccagcga cgtggggtaa taccgttggt
    38701 gacgttatgg aatttgttcg gccatagctg gtgatattcc gggaacagat ctttcaccac
    38761 cagatccgag tgcagcgccg caacaccgtt caccgcgaaa ccgccaacca cacacaggtt
    38821 cgccatatgc acttgtttat cgtgcaccac cgccagtttg gcccacactt tttcatcgcc
    38881 cggccaggtt ttctccacca gcgttttaaa gcgagtatta atttcgttaa taatctgcat
    38941 atggcgcggc agtaagcctt tcaccagttt cacatcccag cgttccagcg cttctggcat
    39001 cagggtatgg ttggtgtaag cgaaagtttt gctggtaatg gcccaggcgt catcccagct
    39061 catctggtgc tcatcgatca gcacgcgcag cagttctgga atcgcgatag ttgggtgggt
    39121 atcgttcagc tgaataactt cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg
    39181 atggcgacgc aaaatatctg ctaccgaaca ggcacactgg aagtactgct gcatcaagcg
    39241 cagcttttta ccggcagtat ggttgtcgtt tggatagaga actttggtca gtttttccgc
    39301 attgatgccc tgctgttcgg cacgcaggaa atcaccgtcg ttaaatttag tcagatcaaa
    39361 cggatgcgcg tgcgtcgcct gccacagacg cagcggctgc gccacgccat tacgatagcc
    39421 gacaacgggg agatcccacg cttgaccggt aatggtaaac tccggctccc agcgtccgtc
    39481 tttcgtcact ttaccgccaa tccctacctg cacatccagt gcttcgttgt ggcggaacca
    39541 cgggtagtta ctgcgatgcc agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga
    39601 ctggcggaac aaaccatatt gatagttcag accgtaaccc gtcgcagact gaccgacagt
    39661 tgccattgag tcgaggaagc acgccgccag acgtcccaga ccaccgttac ccagcgccgg
    39721 gtcgatctct tcttccagca ggtccgtcag attgatgtca taagccttca acgaatcctg
    39781 tacatcctga taccagccga gattcaacag gttgttgccc gtcaggcgac caatcaaaaa
    39841 ctccattgag atgtagttaa catgtcgctg attcgccacc ggcttggcga atggctgagc
    39901 acgcagcatt tcggccagtg cttcactcac tgctagccac cactggcgag gagtcatttc
    39961 agccgcagaa tttaagccat aacgctgcca ctgacgtgaa agcgcttcct gaaattgctt
    40021 atcgttaaaa ataggttgtg acataggagt tccacttttc ttagattttc aacacaacgt
    40081 tatcgctagt ttgccaggct cgatgttgac cttcctcatc ctgcggggga ttaggcaggg
    40141 aggagttgcg gggatgagca aggaaatgtg atctcaacca cttaaagcta gtgcaaacca
    40201 caggattagc agcaaatcaa tgcaatacag cgcagaaaat ctgtatctaa gtgcaaaaaa
    40261 tggccgttgc gtattt
//
LOCUS       DAPPNJ010000014        40005 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000014 DAPPNJ010000000
VERSION     DAPPNJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.014"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            107..457
                     /gene="hypA"
                     /locus_tag="RPM73_000557"
     CDS             107..457
                     /gene="hypA"
                     /locus_tag="RPM73_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDX6518951.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            461..1333
                     /gene="hypB"
                     /locus_tag="RPM73_000558"
     CDS             461..1333
                     /gene="hypB"
                     /locus_tag="RPM73_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDX6518952.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            1324..1596
                     /gene="hypC"
                     /locus_tag="RPM73_000559"
     CDS             1324..1596
                     /gene="hypC"
                     /locus_tag="RPM73_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDX6518953.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            1596..2717
                     /gene="hypD"
                     /locus_tag="RPM73_000560"
     CDS             1596..2717
                     /gene="hypD"
                     /locus_tag="RPM73_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDX6518954.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            2714..3724
                     /gene="hypE"
                     /locus_tag="RPM73_000561"
     CDS             2714..3724
                     /gene="hypE"
                     /locus_tag="RPM73_000561"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDX6518955.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            3798..5876
                     /gene="flhA"
                     /locus_tag="RPM73_000562"
     CDS             3798..5876
                     /gene="flhA"
                     /locus_tag="RPM73_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDX6518956.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(5913..6266)
                     /locus_tag="RPM73_000563"
     CDS             complement(5913..6266)
                     /locus_tag="RPM73_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDX6518957.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(6298..6477)
                     /locus_tag="RPM73_000564"
     CDS             complement(6298..6477)
                     /locus_tag="RPM73_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518958.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            6553..9114
                     /gene="mutS"
                     /locus_tag="RPM73_000565"
     CDS             6553..9114
                     /gene="mutS"
                     /locus_tag="RPM73_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDX6518959.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            9220..9876
                     /gene="pphB"
                     /locus_tag="RPM73_000566"
     CDS             9220..9876
                     /gene="pphB"
                     /locus_tag="RPM73_000566"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDX6518960.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(9927..10694)
                     /locus_tag="RPM73_000567"
     CDS             complement(9927..10694)
                     /locus_tag="RPM73_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX6518961.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            10890..11798
                     /locus_tag="RPM73_000568"
     CDS             10890..11798
                     /locus_tag="RPM73_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDX6518962.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            11795..13057
                     /locus_tag="RPM73_000569"
     CDS             11795..13057
                     /locus_tag="RPM73_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDX6518963.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGLCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            13054..13692
                     /locus_tag="RPM73_000570"
     CDS             13054..13692
                     /locus_tag="RPM73_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDX6518964.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            13697..14473
                     /locus_tag="RPM73_000571"
     CDS             13697..14473
                     /locus_tag="RPM73_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDX6518965.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            14562..15926
                     /locus_tag="RPM73_000572"
     CDS             14562..15926
                     /locus_tag="RPM73_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDX6518966.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(15966..16202)
                     /locus_tag="RPM73_000573"
     CDS             complement(15966..16202)
                     /locus_tag="RPM73_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6518967.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(16213..17640)
                     /locus_tag="RPM73_000574"
     CDS             complement(16213..17640)
                     /locus_tag="RPM73_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDX6518968.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            complement(17640..18233)
                     /locus_tag="RPM73_000575"
     CDS             complement(17640..18233)
                     /locus_tag="RPM73_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDX6518969.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALRDMPNVETHLVMSKWAKTTIE
                     LETPYSAHDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            18380..18787
                     /locus_tag="RPM73_000576"
     CDS             18380..18787
                     /locus_tag="RPM73_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDX6518970.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(18908..19900)
                     /gene="rpoS"
                     /locus_tag="RPM73_000577"
     CDS             complement(18908..19900)
                     /gene="rpoS"
                     /locus_tag="RPM73_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDX6518971.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(19963..21102)
                     /gene="nlpD"
                     /locus_tag="RPM73_000578"
     CDS             complement(19963..21102)
                     /gene="nlpD"
                     /locus_tag="RPM73_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDX6518972.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(21242..21868)
                     /gene="pcm"
                     /locus_tag="RPM73_000579"
     CDS             complement(21242..21868)
                     /gene="pcm"
                     /locus_tag="RPM73_000579"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDX6518973.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(21862..22623)
                     /gene="surE"
                     /locus_tag="RPM73_000580"
     CDS             complement(21862..22623)
                     /gene="surE"
                     /locus_tag="RPM73_000580"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDX6518974.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(22604..23653)
                     /gene="truD"
                     /locus_tag="RPM73_000581"
     CDS             complement(22604..23653)
                     /gene="truD"
                     /locus_tag="RPM73_000581"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDX6518975.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(23650..24129)
                     /gene="ispF"
                     /locus_tag="RPM73_000582"
     CDS             complement(23650..24129)
                     /gene="ispF"
                     /locus_tag="RPM73_000582"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDX6518976.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(24129..24839)
                     /gene="ispD"
                     /locus_tag="RPM73_000583"
     CDS             complement(24129..24839)
                     /gene="ispD"
                     /locus_tag="RPM73_000583"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDX6518977.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(24858..25169)
                     /gene="ftsB"
                     /locus_tag="RPM73_000584"
     CDS             complement(24858..25169)
                     /gene="ftsB"
                     /locus_tag="RPM73_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDX6518978.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(25363..25686)
                     /locus_tag="RPM73_000585"
     CDS             complement(25363..25686)
                     /locus_tag="RPM73_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDX6518979.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(25736..26341)
                     /gene="cysC"
                     /locus_tag="RPM73_000586"
     CDS             complement(25736..26341)
                     /gene="cysC"
                     /locus_tag="RPM73_000586"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDX6518980.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(26341..27768)
                     /gene="cysN"
                     /locus_tag="RPM73_000587"
     CDS             complement(26341..27768)
                     /gene="cysN"
                     /locus_tag="RPM73_000587"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDX6518981.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMLHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(27770..28678)
                     /gene="cysD"
                     /locus_tag="RPM73_000588"
     CDS             complement(27770..28678)
                     /gene="cysD"
                     /locus_tag="RPM73_000588"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDX6518982.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            28930..29967
                     /gene="iap"
                     /locus_tag="RPM73_000589"
     CDS             28930..29967
                     /gene="iap"
                     /locus_tag="RPM73_000589"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDX6518983.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTMIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   30049..31358
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30049..30077
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(31455..31748)
                     /gene="cas2"
                     /locus_tag="RPM73_000590"
     CDS             complement(31455..31748)
                     /gene="cas2"
                     /locus_tag="RPM73_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDX6518984.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGKNRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(31748..32668)
                     /gene="cas1e"
                     /locus_tag="RPM73_000591"
     CDS             complement(31748..32668)
                     /gene="cas1e"
                     /locus_tag="RPM73_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDX6518985.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLINKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(32665..33315)
                     /gene="cas6e"
                     /locus_tag="RPM73_000592"
     CDS             complement(32665..33315)
                     /gene="cas6e"
                     /locus_tag="RPM73_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDX6518986.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRPFVSELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVKGQAEGRDIWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENTRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(33297..34043)
                     /gene="cas5e"
                     /locus_tag="RPM73_000593"
     CDS             complement(33297..34043)
                     /gene="cas5e"
                     /locus_tag="RPM73_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDX6518987.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDTLRNAYQQYQDHFRKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(34054..35109)
                     /gene="cas7e"
                     /locus_tag="RPM73_000594"
     CDS             complement(34054..35109)
                     /gene="cas7e"
                     /locus_tag="RPM73_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDX6518988.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTDYASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(35121..35657)
                     /gene="casB"
                     /locus_tag="RPM73_000595"
     CDS             complement(35121..35657)
                     /gene="casB"
                     /locus_tag="RPM73_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDX6518989.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(35654..37216)
                     /gene="casA"
                     /locus_tag="RPM73_000596"
     CDS             complement(35654..37216)
                     /gene="casA"
                     /locus_tag="RPM73_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDX6518990.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(37314..39971)
                     /locus_tag="RPM73_000597"
     CDS             complement(37314..39971)
                     /locus_tag="RPM73_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDX6518991.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELD
                     NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQ
                     QKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWL
                     THVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQ
                     VIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRSMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQ
                     DPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         9400 a        10794 c        10285 g         9526 t
ORIGIN      
        1 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
       61 tggcacaatt attgcttgca gctggcaata gttaatggga ggcgatatgc acgaaataac
      121 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
      181 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
      241 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
      301 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
      361 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
      421 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
      481 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
      541 cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta aagcgcctga
      601 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
      661 tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
      721 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
      781 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
      841 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
      901 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
      961 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
     1021 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
     1081 acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
     1141 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
     1201 ctttgacgtt gaaaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
     1261 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
     1321 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
     1381 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
     1441 ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat gagcgtaatt
     1501 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
     1561 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
     1621 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
     1681 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgctatct
     1741 ttaaattcgg cctcgaccag ttactgccgg aaaacgttga gtttatccac ggtccggggt
     1801 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
     1861 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggttcgc
     1921 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
     1981 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
     2041 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
     2101 tttacttctt ctgccagcat attacgctta tcccgacgct tcgcagtttg ctggaacagc
     2161 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
     2221 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtggct ggtttcgaac
     2281 cgcttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
     2341 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
     2401 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
     2461 aatcttctgg tgtacacctg acgccagatt atcaacgatt cgatgccgaa gcgcatttcc
     2521 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcacg ttgtggtgaa gtattaacgg
     2581 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
     2641 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
     2701 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
     2761 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagaacagga
     2821 agatcaggca cgtcttgagc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
     2881 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
     2941 gatttgcggc acagccaatg acgttgcggt cagtggcgct attccgcgct atctctcctg
     3001 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
     3061 ggcagaaacc gcgcgcgcgg caggcattgc catcgtcact ggcgatacta aagtggtgca
     3121 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
     3181 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttactggtta gcggcacgct
     3241 cggggaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
     3301 ggtcagcgac tgcgcggtac tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
     3361 gaaagcgctg cgtgatgcca cccgtggtgg tgtgaacgcg gtagttcatg agttcgcggc
     3421 agcctgcggt tgtggtattg aaatttcaga agcggcactg ccggttaaac ctgctgtgcg
     3481 cggcgtttgt gaactactgg gactggacgc ccttaacttt gccaacgaag gcaaactggt
     3541 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
     3601 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
     3661 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
     3721 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
     3781 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
     3841 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
     3901 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
     3961 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
     4021 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
     4081 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
     4141 caaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct
     4201 gtgaatttat tcgttatgac gatcgcccct ggagcgaaaa agagttcaat cgtctgcaaa
     4261 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gttaacaatg
     4321 tcgactatga gttgttatgc cgggaacgcg ataacttccg catcctggtc gccatcacca
     4381 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
     4441 attactattt cgacattgac gatatcagta tcgtcttacg cagccaccgt aaaaacaaac
     4501 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
     4561 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
     4621 atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
     4681 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
     4741 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
     4801 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
     4861 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
     4921 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
     4981 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta
     5041 aagagctgat tgcccgcgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
     5101 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
     5161 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
     5221 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
     5281 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
     5341 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
     5401 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
     5461 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
     5521 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
     5581 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc ggtattgcta acacgcggta
     5641 acgtgctgca gctgtcattg ccagatattg ttttaccgga acctgaaacg ccgcctgccg
     5701 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgttcgc gtgctgaaag
     5761 aaaccaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
     5821 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
     5881 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
     5941 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
     6001 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
     6061 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
     6121 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
     6181 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
     6241 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgt atttatatta
     6301 catcttaatc ttaaagggca ctatgactct aaagaagaaa ggttcgccaa ccgacgcaat
     6361 tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac
     6421 cagcatcaag aactcaccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg
     6481 cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc
     6541 ggacataacc ccatgagtgc aatagaaaat ttcgacgccc atacgcccat gatgcagcag
     6601 tatctcaagc tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt
     6661 tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc
     6721 aaacgcggtg cttcggcggg agagccgatc ccgatggcag ggatccccta ccatgcggtg
     6781 gaaaactacc tcgccaaact ggtgaatcag ggcgagtccg ttgccatctg cgaacaaatt
     6841 ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca
     6901 ggcaccatca gcgatgaagc cctgttacag gagcgtcagg acaacctgct ggcggctatc
     6961 tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc
     7021 ctgagcgaac cggctgaccg ggaaacgatg gcggcagaac tgcaacgcac taatcctgcg
     7081 gaactgctgt atgcagaaga ttttgctgaa atgtcgttaa ttgaaggccg tcgcggcctg
     7141 cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa
     7201 tttggcactc gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct
     7261 gccggttgtc tgttgcagta tgcgaaagat acccaacgta cgactctgcc gcatattcgt
     7321 tccatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac gcgtcgtaat
     7381 ctggaaatca cccagaacct ggcgggtggt gcggaaaata cgctggcttc tgtgctcgac
     7441 tgcaccgtca cgccgatggg cagccgtatg ctgaaacgct ggctgcatat gccagtgcgc
     7501 gatacccgcg tgttgcttga gcgccagcaa actatcggcg cattgcagga tttcaccgcc
     7561 gagttgcagc cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct
     7621 ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg
     7681 gagctgcgtg cacagttaga aactgtcgat agtgcaccgg tacaggcgct acgtgagaag
     7741 atgggcgagt ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgcca
     7801 gtgctggtac gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg
     7861 cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa
     7921 cgtaccggcc tggacacgct aaaagttggc tttaatgcgg tgcacggcta ctacattcaa
     7981 atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa
     8041 aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca
     8101 aaaggcaaag cactggctct ggaaaaacag ctttatgaag aactgttcga cctgctgttg
     8161 ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtg
     8221 aacctggcgg aacgggccta taccctgaac tacacctgcc cgacctttat tgataaaccg
     8281 ggcattcgca ttaccgaagg ccgccatccg gtggttgaac aggtgctgaa tgagccgttt
     8341 atcgccaacc cgctgaatct gtcgccgcag cgtcgcatgt tgatcatcac cggtccgaac
     8401 atgggcggta aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc
     8461 ggcagctatg taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc
     8521 gtaggcgcgg cagatgacct ggcttccggg cgctcaacct ttatggtgga gatgactgaa
     8581 accgccaata ttttacataa cgccaccgaa tacagtctgg tgttaatgga tgagatcggg
     8641 cgtggaacgt ccacctacga tggtctgtcg ctggcgtggg cgtgcgcaga aaatctggcg
     8701 aataagatta aggcattgac gctgtttgcc acccactatt tcgagctgac ccagctaccg
     8761 gagaaaatgg aaggcgtcgc caacgtacat ctcgatgcgc tggagcacgg cgacactatc
     8821 gcctttatgc acagcgtgca ggacggcgcg gcgagcaaaa gctacggtct ggcggttgct
     8881 gctctggcgg gcgtaccgaa agaggttatt aagcgcgcac ggcaaaaact gcgtgagctg
     8941 gaaagcattt cgccgaacgc cgccgctacg caagtggatg gtacgcagat gtctttgctg
     9001 tcagtgccag aagaaacttc gcctgcggtc gaagcactgg aaaatctcga cccggattca
     9061 ctgactccgc gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaataacaa
     9121 ttcccgatag tcttttgcta tcgggaatat taacgacaac tgacgaatca aataaaaata
     9181 ccctgtataa taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa
     9241 aaaatcaatg cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat
     9301 cagttattac aatcccgttt acatcaactc tctttttacc ctgaaaccga cttacttatt
     9361 tccacaggcg ataatattga tcgtgggccg aagagtctta acgtgctgcg cctgctaaac
     9421 caaccctggt ttacgtcggt taaaggcaac cacgaagcga tggcgttgga tgcattcgaa
     9481 actggcgatg gaaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat
     9541 tcagagcaac aagaggcaat agatctgttg ctgaaattcc atcaccttcc acatattatt
     9601 gaaatcacta acgacaacat aaaatatgtc atcgcacatg cagattatcc ggggagtgaa
     9661 tatctctttg gtaaagaaat agcggagagc gaattactct ggcctgttga tcgtgtgcag
     9721 aaatcgctta atggcgagtt acaacaaata aacggcgctg attattttat atttggacat
     9781 atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac cggcacgccg
     9841 aaaagcgggc ggctgtcatt ttataaaata aagtagtctc atgcttcttc tgtgaagcat
     9901 gagtaaccaa atgttattgc aggccattat tcatttttcg caaccagcaa agagagatcc
     9961 tgcttcgcca gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtgat aatctgatca
    10021 aactcgctta atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag
    10081 accaaaactc gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc
    10141 gctggcgtag aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc
    10201 atcaggctgc gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac
    10261 actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gttcgcaata
    10321 acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct
    10381 aacgtggtcg ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc
    10441 agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt
    10501 tcatgcgcca cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc
    10561 tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg acacattcat tctgtccgtt
    10621 agttcagcaa tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc
    10681 tctacgggta tcaacttttg ctccttcctt tgtcccgctg acattctacg ctatttgcct
    10741 gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa aataacacac aatgttaatt
    10801 tatgtgaatc agctcaccat atcgttatct tccagcgctt atattcacaa tatcaaacaa
    10861 aatatcactt aaattaacaa ggagagcaga tgaaaacggg atctgagttt catgtcggta
    10921 tcgttggctt agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggtcttt
    10981 ctacctgggg cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt
    11041 gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg
    11101 tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc
    11161 tgaaacccgg tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa
    11221 ttgctaccgc tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg
    11281 cagtaaaagc cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg
    11341 aacgactggc acccgtgctg gaagccgttg ccggaaaagt ttatcgcata ggtgcagaac
    11401 cgggactagg ttcgaccgta aaaattattc accagttgtt agcgggcgta catattgctg
    11461 ccggagccga agcgatggca cttgcagccc gtgcggggat cccgctggat gtgatgtatg
    11521 acgtcgtgac caatgccgcc ggaaattcct ggatgttcga aaaccggatg cgtcatgtgg
    11581 tggatggcga ttacaccccg cattcagccg tcgatatttt tgttaaggat cttggtctgg
    11641 ttgccgatac agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca gcattgaata
    11701 tgttcaccag cgccagtaac gcaggttacg ggaaagaaga cgatagcgca gttatcaaga
    11761 ttttctctgg catcactcta ccgggagcga aatcatgatc aagattggcg ttatcgccga
    11821 tgattttacc ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc taccaacggt
    11881 acaaattaac ggtgttccaa caggtaaaat gccggaagca atcgacgcac tggtgatcag
    11941 cctgaaaacg cgctcctgtc cggtggttga agccacacag caatcgctgg cggctctgag
    12001 ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa tactgctcta ctttcgacag
    12061 tacggcgaaa ggtaatatcg gcccggttac cgatgcatta atggatgctc tcgacacgcc
    12121 gtttacggtc ttctctccgg ccctgccggt caacggacgt acggtttatc aggggtattt
    12181 gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc catcacccgg taaatcccat
    12241 gaccgacagc tatcttcccc gtctggttga agcgcaatcc acagggctct gcggcgtcgt
    12301 ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt caagagctgg ctcgcttaca
    12361 gcaagagggc taccgctacg cggtgcttga tgcgctgacc gaacaccatc tggaaattca
    12421 gggagaagcc ttgcgcgatg ccccactggt aacgggtggt tctggtctgg cgattggcct
    12481 ggcccggcag tgggcgcaag aaaacggtaa ccaggctcgc gaagcagggc atccgctcgc
    12541 tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg accaaccgcc aggtggcaca
    12601 ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca cgctgcctct caaccgaaac
    12661 tctggccgct tatgcacacg aactggcaga gtgggttctg ggccaggaaa gtgtacttgc
    12721 tccactggtt tttgccaccg ccagcactga cgcattggca gcaattcaac agcaatacgg
    12781 tgcacaaaaa gccagtcagg cagtagaaac tctattttct caactagcgg cgcggttagc
    12841 agcggaaggc gtgacacgct ttattgtcgc aggcggtgag acctccggcg tagtcacaca
    12901 gagcctgggc ataaaagggt ttcatattgg cccaaccatt tcccccggcg tgccgtgggt
    12961 aaacgcactg gataagcctg tctcactcgc ccttaaatct ggcaacttcg gtgatgaagc
    13021 ctttttttca cgagcccaaa gagagttttt atcatgagcg atttcgcaaa agtagagcag
    13081 tctttgcgag aggagatgac gcggattgcc agttcattct ttcagcgcgg ctatgcaacc
    13141 ggttcggctg gcaatctgtc gctgctttta cctgacggga atttactggc gacaccgaca
    13201 ggttcatgcc tgggcaatct cgatccgcag cggctttcca aagtcgccgc ggatggcgaa
    13261 tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc atctggcgct gtatcgcaac
    13321 aatccgcgct gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc gctttcctgc
    13381 ctgcaagggc tggacagcag caacgttatt cgtccgttca caccatacgt ggtgatgcgg
    13441 atgggaaatg tcccgctggt gccttattac cgaccgggcg ataaacgcat cgcacaggat
    13501 ctggcggaac tggcagcaga caatcaggct tttttactgg caaatcatgg cccagtggtt
    13561 tgcggtgaaa gcctgcaaga agccgccaac aatatggaag agctggagga aacggcaaag
    13621 ctgattttta ttctcggtga ccgcccgatc cgttatctga ccgcaggtga aattgcggaa
    13681 ttaaggagtt aatgcaatgc ctcgttttgc agctaattta tccatgatgt tcaccgaagt
    13741 gccttttatt gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg tggaatttct
    13801 gtttccctat gactactcca ccctgcaaat ccaaaagcaa ctggagcaaa accatctgac
    13861 actggcgctg ttcaataccg ctcccggaga tattaatgcg ggggaatggg gattatccgc
    13921 ccttcccgga cgtgagcacg aagcacacgc agacatcgac ctggcactgg aatatgcatt
    13981 ggcgctcaat tgtgaacaag tccatgtgat ggcaggcgtc gtgcccgctg gcgaagacgc
    14041 tgaacggtac cgggcagtat ttatcgataa tctccgctac gctgccgacc gctttgcgct
    14101 acacggcaag cgaatattag ttgaagcact cagccccgac gttaagcctc attatctctt
    14161 ctccagccag tatcaggcac tggctattgt tgaagaggtt gcgcgagata acgtgtttat
    14221 tcaactagat acttttcatg cgcaaaaagt agatggcaac ctgacgcatt taatccgcga
    14281 ctacgctggg aaatatgcgc acgtacaaat tgccggacta cctgatcgac atgaaccgga
    14341 cgatggagaa atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg gatatcaggg
    14401 ctggatcggt tgtgaatata aacctcgtgg cctcaccgaa gaagggcttg gctggtttga
    14461 cgcctggcgc taattcgtta aatccctaat tacaacgtac ccatacatcc ccccatctac
    14521 ggatggggta ggttaactat aactttcaga cagggtttcc catgtccaca attacattgt
    14581 tatgcattgc gttagctggc gtaatcatgc tgttgctgct ggtcatcaag gcaaaggtac
    14641 aaccattcgt tgctctgctc ctcgtcagcc tgttagtcgc acttgcggcg ggtataccgg
    14701 cgggcgaagt gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt tccgtcacta
    14761 ttattattgg tctgggcgct atgctggggc gtatgatcga acactctggt ggtgcagagt
    14821 cactggctaa ttatttcagt cgcaagttag gtgacaaacg aactatcgct gcgctgactc
    14881 tggcagcgtt cttcctcggt attcccgtct tctttgatgt cggctttatt attcttgcgc
    14941 caatcattta cggttttgcc aaggttgcca aaatatcgcc actcaaattt ggcctgcctg
    15001 tcgctggaat catgctaact gttcatgtgg cggtaccgcc gcatccaggc cctgtcgccg
    15061 cagcggggtt actccacgca gacatcggct ggctaaccat catcggtatt gcgatttcta
    15121 ttcccgtagg gattgttggc tactttgcag cgaaaataat caatacgcgc caatatgcga
    15181 tgtcggtgga agtactggaa cagatgcaac tggctccggc cagtgaggaa ggcgcaacaa
    15241 aattaagcga taaaataaat ccaccgggcg tcgcgctggt cacctcgcta attgttattc
    15301 ctatcgcgat tatcatggcg ggtacggttt ccgcaacact gatgccgcct tcgcatcccc
    15361 tgcttggtac gctacagctg atcggctcac caatggtagc tctaatgatt gcgctggtgc
    15421 tggcattctg gttattggct ttacgtcgcg gctggagctt acaacacacc agcgacatta
    15481 tgggctcagc ccttcccact gcggcggtag tgattttggt tactggtgct ggaggggtat
    15541 ttggcaaagt gctggtggaa tcgggcgtcg gcaaagccct tgccaacatg ctacaaatga
    15601 ttgacctgcc tctgttacca gccgcattta ttatttcact ggcgctgcgt gcatcgcagg
    15661 ggtcagcaac cgtagcaatc ctgacaaccg gcgggttact ctcagaagcg gtgatgggat
    15721 tgaatccgat tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg cttggcgcgt
    15781 cacatattaa tgactcaggg ttctggattg tgaccaaata tctggggttg tcggtagcag
    15841 acggtctgaa aacctggaca gtgttaacga ccattctcgg ttttaccgga ttcttaatta
    15901 cctggtgcgt atgggcggta atttgacctt aataaaaagg tccgatgggc atcggacctt
    15961 tttatttagc gcttaccttc cgccagcagc ggcgggatgc tcggcaccat tggcgcatca
    16021 tcaatatctt tctgcgtcat gcggaacgct tccgggtaat gttcgcgact ggtgcggcgt
    16081 agcggttcgg tatcgcgcca ggtataaagg caatgctggc actgatatac cgtccagaca
    16141 tctttcaccg gcgatttcac catcacttca atctgttcat cggcacaacg tggacaaatc
    16201 atcttgttct ccttatttac gtgcagccag catagtggtc agtttttcag cccaggcttt
    16261 ggtttccggc aaatccacca ccggctggct gtagtgacca cggttgtccg gggcgacagg
    16321 cgtggtagcg tcgataatca gcttgtcggt gatccccgcc gggcttgagc cagggtcgag
    16381 ttccagcacg gacatattcg gcaactgcac caaatcccct gccgggttta ctttcgagga
    16441 gagcgcccac atcacctgcg gcaggttgaa cgggtcaacg tcttcatcga ccataatcac
    16501 catctttacg tagcccagac cgtgcggcgt ggtcatcgca cgcaggccca ccgcgcgggc
    16561 aaagccgccg tagcgttttt tggtggagat aatcgccagc aggccgtggg tgtacatggc
    16621 atttaccgcc tgcacttccg ggaactcggc tttcagttgt tgatacagcg gtacgcaggt
    16681 ggctggcccc atcaggtagt cgatttcggt ccacggcata ccgagataga gtgattcgaa
    16741 aatcggtttg ctgcgataag agactttatc gatgcgcacc acggtcatgt tgcgcccacc
    16801 ggagtagtgc ccggtaaact caccgaacgg cccttcgatt tcgcgtttac ggctttcgat
    16861 aaccccttcg aggatcactt ctgaacccca cggcacatca aaaccggtca gcggggcggt
    16921 ggcgatcggg tacgggcttt cacgcagcgc gcctgccatt tcgtactcgg actgatcata
    16981 tttcagcggc gtggccccca tcagcgtgat gatcggatcg ttaccgagcg tgatcgcaat
    17041 cggcagatct tcaccgcgct cttctgcttt atgcagatgc agggcgatat cgtgcatcgg
    17101 caccggttgc aggccgagct tacgcttgcc cttcacttcc atgcggtaga tgccgacgtt
    17161 ctgcttgccg aagttatccg ggtcgagcgg atcgcgggaa accacgcacg ctttgtcgag
    17221 atagaaaccg ccatcgccat cgtttaaacg aaacagcggc aggatatcga acaggttgat
    17281 ctcgtcgcca tcaacggtgt tctgcgccca ggctggattg gcgcggcgct ccggggcaat
    17341 cgggaagtta tcccagcggc ggataaactc atcaatctgc tttttaaccg gggcatttgg
    17401 cggcaggccg agggaaatcg cgtggttctg ccaggaaccg atggtgttca tcgccacgcg
    17461 ggcatcggta aagccgcgaa tattatcaaa ccacagcgcg ggcgcgccgt cgccgatacg
    17521 cccggtggcg ttagctgctg ctgccagatc cggctcagcg ttcacctctt cgctgatttt
    17581 cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat caaatgccat
    17641 tattcattct cctgagaaaa attccgggcc tgcggcaatc cttgccagcg cctggcatgg
    17701 ggatgttcga ggccaaattg atccagcacg cgggctacca catggtggac aatgtcatct
    17761 accgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac gcccatgcgt
    17821 gaaagtgcga gcatattttc gagatggatg gtgctaagcg gcatttcacg cggcaccagc
    17881 accagtttgc ggccttcttt gagcacgacg tccgccgcgc gccctaccag gccatcggcg
    17941 taaccagcgc ggataccggc gagcgttttc atactgcacg gaataacgat catgccgtcg
    18001 gtacgaaaag aaccggatga gatgatcgcc gcctgatccg ccgggttatg gctgaagtct
    18061 gcgagggcag caacatcgtg agcgctgtaa ggcgtttcta gttcaatggt ggttttcgcc
    18121 cacttcgaca tcaccagatg agtctcgaca ttcggcatat cccgcagcgc ttgcagtaat
    18181 gccacaccaa gaggcgcacc ggtagcccct gtcatcccga cgatcagttt cattgcttat
    18241 cctcttaatt gttcgtatac gaacgttatt attaacatac tccttcattt ccacagcggc
    18301 aaggattgaa aagtaacaac atcttcttcg tcactaaaaa accgctatga tagcgacaga
    18361 gaattagccc ggagtttaca tggcgttacg aaataaagcg ttccatcagt tacgacagct
    18421 ttttcagcag cacacggctc gctggcagca cgagttacct gacctgacca aaccacagta
    18481 cgcggtgatg cgcgccattg ctgataaacc tggtatcgaa caggtggcac tgatagaagc
    18541 agctgtcagc accaaagcaa cgttagcaga aatgttggca agaatggaaa atcgtggctt
    18601 agtcagacgt gaacatgacc ccgctgataa gcggcgacgc tttgtctggt tgactgccga
    18661 aggcgaaaag atacttgctg cggcgatacc cattggtgac agcgtggatg aggaattttt
    18721 ggggcgtttg agtggagaag agcaggagct gtttatgcag ctggtgcgca agatgatgaa
    18781 cgcatagggg gcaaatttcc ggatgcgacg ctatcgcgtc ttatccagac tacaaataca
    18841 ccgactcacc aaccgaaaga aaaaaggcca gcctcgcttg agactggcct ttctgacaga
    18901 tgcttactta ctcgcggaac agcgcttcga tattcagccc ctgcgtttgc aggatttcgc
    18961 gcaaacggcg caggccttca acctgaatct ggcgaacacg ttcacgggtg aggccaattt
    19021 cacgacctac atcttccagt gttgccgctt cgtaccccag caaaccgaat cgacgtgcca
    19081 gcacttcacg ctgtttggcg ttcagctcga acagccattt gacgatgctc tgcttcatat
    19141 cgtcatcttg cgtggtatct tccggaccgt tctctttttc atcggccagg atgtccagca
    19201 acgctttttc ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac
    19261 gaagcatacg gctgacgtca tcaactggct tatccagttg ctctgcgatc tcttccgcac
    19321 ttggttcatg gtccagctta tgggacaact cacgtgcggt tcgcaggtaa acgttcagct
    19381 cctttacgat gtgaatcggc aaacgaatag tacgggtttg gttcataatc gcccgttcaa
    19441 tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa
    19501 acttctctac cgcgcggatc agccccaggt tgccctcttc gataaggtcc agcaacgcca
    19561 gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta ctctcgatca
    19621 tccggcggcg agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa acttcttctt
    19681 cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc gcgtccaaca
    19741 cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta tcactgggtt
    19801 cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca aattccgcat
    19861 cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc ctacccgtga
    19921 tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct gcggcaaata
    19981 acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc gtgttgaact
    20041 ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt gttgttcccg
    20101 gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat gtttgatgat
    20161 aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacgcggc catctgcggt
    20221 cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc ccccctcaga
    20281 agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg agataggcgt
    20341 actggttgat gtactgctga cagttggctc ggttgtgctt gctgttggta ccgttacagg
    20401 cgctgtgacc gtggtcgcag ctggcttgtt gttcggcaac attttgttag cactctgttc
    20461 acccgaagat tcagaatacg taattgtcgg ttgcgacgca acagcaacgg tggaattttg
    20521 tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc
    20581 agtgattggc gtaccggaag cattacccac ctgcaaggtc tgaccaacgt tcagcgcgta
    20641 tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag taatccaggc
    20701 gatatagaaa agtgtgtcgc cttttttcac ggtataggta ctgccgctat aactgccttt
    20761 cggaatgttc ccatactgac ggttatagac gatgcgtccg ttttccatct gtaccggctg
    20821 ctgagctact ggctgcactg gctggatttg cggttgttga gtagcctgaa tttgtggctg
    20881 ctgtaccggc tgaatttgcg gttgctgggc tgtagacgtc gtccccattt tcggcggcgg
    20941 cgtaatcaac ataccagaat tggtatttgc aggcgcattg ccattaacgg agctgaccgg
    21001 tgccggtgga tttgaagtgt cagaacagcc tgccagccat agcgaaacca gtgacaaagc
    21061 cgcaatgcgg cgaacggtga attttgggct tcccgcgctc atttatcccc caggaaaaat
    21121 tggttaataa ccagtgacat aattaccgtg caaggcaccc tactgaacac tggaaaagat
    21181 gttcacgata cgctgacctg cggcaaaata accaggaaaa atccaggtat ttcctcacgt
    21241 tttaagccag ctcacccttc actaaaggga caaagcgcac ggcctccacg gtatcgataa
    21301 taaattcgcc tccccgacga cgcacccgtt tcaaatactg gtgctcctcc cctacgggta
    21361 agacgagaat cccgccttcg tccagctgcg tcattagcgc agttggaatt tccggcggtg
    21421 ccgccgtaac aatgatagcg tcaaacggcg cgcgtgcctg ccaaccttgc catccatcgc
    21481 catgacgggt tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc gcctgccact
    21541 gcaaaccttt aatccgttcg accgagcaaa catgctggac aagatgcgcc aggattgccg
    21601 tttgatatcc cgaaccggtg ccaatttcca gcacccgcga ctgcggcgtc agctcgagta
    21661 attcggtcat tcgcgccacc atatacggct gcgaaattgt ctgcccctga cctatcggca
    21721 aggcgatatt gtcccaggct ttttgttcaa acgcttcatc aacgaatttt tcacgcggca
    21781 cggcagcaag tgcattcagc acctgctcat cctgaatacc ttgcgcacgt aattgatcca
    21841 gaagtgcttg tacgcgtctg cttaccattg cgtgccaact cccacgctgt ttaaccagtc
    21901 tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg tgatggagac
    21961 atagccctca tctaccgcag caaaatcggt ccccggaccg gcatcacatt taccgcccgg
    22021 cgggccaatc cagtacagcg tattgccgcg cggatcttgc tgcgggatca cctgatctgc
    22081 cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg atttgatcca agggtaaatc
    22141 cggaacgtta atattaagaa tacgcccggt gcgcaacggc tctttacaca gtgcgcgcaa
    22201 aattgaacag gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc
    22261 aagcgccgga aaacctaaat gacggccttc catcgcggcg gctaccgtac cggaataaat
    22321 aacatcatcc cccagattcg gcccggcgtt aattccggac acaacaatgt ccgggcgcgg
    22381 acgcatcaga gcattcacgc caagatagac gcaatcggtc ggggttccca tttgcacagc
    22441 aatatcacca ttttcaaagg taaacgtgcg cagggaggat tccagtgtca gagaatttga
    22501 agcgccgctg cggttacgat cgggggcgac cacctgaacg tcagcaaact cacgcaaggc
    22561 tttcgccagc gtttgtatac cgggtgcatg taccccgtca tcattactca gcaatatgcg
    22621 cataatcacc tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa ctacccgccg
    22681 gaagccagaa acggatctct acggtgacgt catcccacca attccagctt aattgttgcg
    22741 gatacagcag catcgctctg cgcgcggctt caactttttc gcgcaccagt aaagcttgta
    22801 attcagtttc tgcggcgaca gctgcttgtt cgaatgccag cgcttcacgc tgagttcccc
    22861 attcgccact gcccggcaat gcggcggtta tcatcagctc tttatcgttg acgcgacgct
    22921 gtaattctgc cagttcttcg gtggttgcga caaaccagct accacgtccg gctaattgta
    22981 gcgcatcacc gtcaacaact tgattaacgt ctgctttttt gaggcgctca gcaacaatct
    23041 gattaaacaa cgcactgcgg gctgccgaca accaaaaact ccgtttattg cgatcgcgca
    23101 ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa gttgctaccg ccaatcccaa
    23161 aacgttgggc accgaagtag ttcggtacac cttttacgca aatatcgatc agacgttgtt
    23221 caacgtcatc gcgattgctc acttcgcgca gaaccagggt aaaggcgtta cctttcagcg
    23281 cgcctaaacg cagcttacgc ttgtgccgcg catactccag cacctggcag ccttccagtt
    23341 gaaaggcgct cagatcgggc atttccttgc ctggcacgcg agcgcataac cactgttccg
    23401 taacagcatg tttgtctttt tgcccagcga agctgacttc acgggcatga attttcagga
    23461 atttcgccag tgcatccgcc acaaaacggg tattgcagcc gtttttgagg attctaacca
    23521 gaatatgctc accttcacca tcaggctcaa agcccaaatc ttccaccacc acaaagtctt
    23581 ccggattggc tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa gtgagattat
    23641 caaactcaat cattttgttg ccttaatgag tagcgccacc gcttcacagg caatcccttc
    23701 cccacgtccg gtaaatccaa gtttttccgt agtagtggct ttcacgttaa catcatccat
    23761 atggcagccg agatcttcgg caataaacac gcgcatttgt ggaatgtgcg gcaacatctt
    23821 cggtgcctga gcgatgatag tgacatcgac gttgcccagg gtatacccct tcgcctgaat
    23881 acgacgccag gcttcgcgta gcagctcgcg gctgtcggca cctttaaatg ccggatcggt
    23941 atccgggaac agcttgccga tatcccccag cgccgccgcg ccaagcaatg catcggtcaa
    24001 cgcatggagc gccacgtcgc catcagaatg cgccagcaat cctttttcgt aaggaatgcg
    24061 tacgccacca atgataattg ggccttcacc gccaaaggca tgtacgtcaa aaccgtgtcc
    24121 aattcgcatt atgtattctc ctgatggatg gttcgggtga ggtaaaactc ggccagtgcc
    24181 aaatcttccg ggcgcgtgac tttaatgtta tccgcacggc cttcgaccaa ctgaggatgg
    24241 aatccgcaat attccagcgc cgaggcttcg tcggtaatag tcgcgccttc atttagagcg
    24301 cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat
    24361 aagccgttgc gatcaacggt atgagcaatg gcatttttgc ccggttcggc acgtttcatc
    24421 gtatcgcgca ctggtgcggc taggatccct cccgtgcggc tggtttcgct caacgccaac
    24481 aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg cgtcatgcac caatacccac
    24541 tgcgcgtcgc cagcggcttt caaacctgcc agcacggaat cggcacgctc atcaccgcca
    24601 tctacaacgg tgatttgcgg atgattcgcc agaggaagtt gtgcaaaacg gctatcgcca
    24661 ggacttatgg caatgacgac acgtttcacc cggggatgcg ccagcagcgc atgcaccgag
    24721 tgttcaagaa tggtttgatt accgattgag agatattgct taggacattc cgtttgcatt
    24781 cgacggccaa atccggccgc cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg
    24841 ttaattcccg ggctgattta tcgattgttt tgccccgcag actgtgcgcg cttcgacgcg
    24901 tcaggcacca gacgataaaa agtttcgccc ggcctggtca tgctgagttc attacgcgca
    24961 cgctcttcga gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa aagttgatcg
    25021 tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg ccacatcatc attgacgcgg
    25081 gtatagtcat gtataccgtt cttaccgaac cacagcgaat actgtagcca gaccagaata
    25141 gccagcaaca gcagcgttag tttacccatc ctgccccctg aaaaacggca tcatcatccc
    25201 atgcatccga agacgactct acatcctctg ttggggatac cgcgacaacg cgggcaaatg
    25261 taccacattt gtccattgtt acgtataccc agggcgtgca gaacataatc tcattattag
    25321 ttacggtttg aattatgaac agaggagaca agaaagtaca aattagccca gtagccacat
    25381 aaacagtgcg ccaaacataa tgcctactgt caccagggtg aaaacaatac tgtagcgtag
    25441 ctttccgtcc atcaatgaat gcagcgcaat ccccaccact accgcgacgg gcatcagcgc
    25501 cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg ttagagccat aaatcaacat
    25561 cggcatcgcc agcgcaaata accaggagat aaaaccgacc acggcaccag gcagtgacca
    25621 tgtggtttct tcatcctcag taaggctgtc gttatttgtt agtgtaatgt tatggctatt
    25681 acgcatattt gatcctgtta ctttgacgaa ccgggcatgg aaacccggtg gtgtctcagg
    25741 atctgataat atcgttctgt ctcaacagat ctaataattg ctgtaccaaa tttgttacta
    25801 attgttcacc attgagatga atttctgccg attcaggcgc ttcgtaaacg gaatctattc
    25861 ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata taagcctttg ggatcgcggg
    25921 cttcgcaaat cgccagcggc gtatcgacaa acacttcgat aaagcgccct tctcctacgc
    25981 gttcacgaac catctggcgt tcggcgcggt gtggcgagat aaatgcggtc agcactacca
    26041 gtccggcttc aaccatcaaa ttcgccactt caccgacgcg acggatattc tctttacgat
    26101 cggcatcgct aaaaccgaga tcgctgcata atccgtggcg aacattatcg ccatccagca
    26161 gatacgtact gacgccgagt ttatgtaacg cctcctccag cgccccggcg accgttgatt
    26221 taccggaccc ggagaggccg gtaaaccaca gcactacacc acgatgaccg tggtgtagct
    26281 cgcgttgttg cacagtgacc ggatggctat gccagacgac gttttcgtca tgcagcgcca
    26341 ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga aagtggcgac gaaccagagc
    26401 attcaattcc agttcgaatg cactgaattc agatggcgca gcagttgcct ggctaactgg
    26461 ctcgtgcagc ataccggcac ccacggtcac attgctcagg cgatcgataa aaatcagccc
    26521 acccgtcacc ggattttgtt gataacgatc taacaccagc ggctcgtcaa aagtgagatc
    26581 cacgaggccg atcccattca gtggcaggtt ttcaacttca cgctgggtaa ggttattaat
    26641 atcaacctga tagcgaatgc catcaatacg cgcgcgcgtc ttcttaccgg caattttgat
    26701 gtcgtaactc tggcccgggg aaagcggctg ttccgccatc cataccacat ccaccgacgc
    26761 gctctgcacc gccggtaacg cttcgtctgc cgccagcagc agatcgccac ggctgatgtc
    26821 gatctcatcc gtcagcacca gggtgatcgc ttctccggca aaggcttctt cgcgatcacc
    26881 atcaaaagtc acgatccgcg cgacgtttga ttccacacca gagggcagca ctttgacacg
    26941 ttgcccgact tccacgcgac cggatgccag cgttccggcg tagccacgaa aatcgagatt
    27001 cgggcgatta acgtactgca ccgggaagcg cattggctgg gcatccacca ctcgctggat
    27061 ctccacggtt tccagcactt cgagtagtgt tggaccgctg taccacggca tactttcact
    27121 ttgcgaagcc acgttgtcgc cttccagtgc agagagcggc acaaagcgga tatccagatt
    27181 accaggtaac tgttcggcga aagtcaggta gtcttcacga atgcgggtga atgtctcttc
    27241 actgtaatcc accagatcca ttttgttgat cgctacgacc agatgtttga tccccagcag
    27301 cgtggagata aaactgtgac gacgggtttg atcgagcacg cctttacggg catcgatcag
    27361 taagatcgcc agctcacacg ttgaggcgcc ggtcgccata ttgcgggtat actgctcgtg
    27421 cccgggggtg tcggcgataa taaatttacg cttctcggta gagaaatagc ggtaggccac
    27481 atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg tccaccagca gagccagatc
    27541 cagtttttcg ccctgggtgc cgtgacgctt actgtcgtta tgcagcgatg agagctgatc
    27601 ttcgtagatt tggcgggtat cgtgcagcag gcgaccaatc agggtacttt tgccgtcatc
    27661 gacgctacca caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc
    27721 ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc
    27781 cctgacgttt tttcagctcc atagaccccg cctggtcgcg gtcaatcacg cggccctgac
    27841 gttcactggt ggtggaaacc agcatctctt cgatgatttc cggcagtgtt tgtgcatttg
    27901 actccaccgc accggtcagc ggccagcagc ccagcgtacg gaaacgcacc atccgttttt
    27961 taatcacttc gcccggttgc aggtcgatac ggttgtcatc aatcatcatc aacataccgt
    28021 cgcgttccag aaccggacgt tccgcagcga gatatagcgg aacaatgtcg atattttcca
    28081 gccagatgta ttgccagata tcctgctcgg tccagttaga gagcgggaag acgcggatgc
    28141 tttcgccttt gttaatttgc ccgttgtagt tgtgccacag ctccgggcgc tgatttttcg
    28201 gatcccagcg atggaagcgg tcacggaaag agtaaattcg ctctttagcg cgggatttct
    28261 cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc gtatttgttc agcgcctgtt
    28321 tcaggccttc agttttcata atatcggtat gtttcgcgct gccgtgcacg aatggattaa
    28381 tccccatcgc cacgccttcc gggtttttat gcaccagcag ttcgcagccg taggctttag
    28441 cagtacgatc gcggaactca tacatctcgc ggaatttcca gccggtatcg acatgcagca
    28501 acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg
    28561 aatctttacc gatagagtag agcatcaccg gatttgagaa ttctgccgcc acctcgcgaa
    28621 taatgtggat gctttccgcc tccagttgcc gcaggtgagt aagtcgtatt tgatccataa
    28681 ccgttccttt gcaataccgc tattttcttg ccatcagatg tttcgactat agggagcgta
    28741 agagaacgaa tgaaattacc aattagaatg agtagttcct taacggaata acgatttggc
    28801 aaagctaata tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga agatttgaaa
    28861 ttgttttagc gcagcggcag tttcatacta tggcggtaaa aaaatttgca tggtatttaa
    28921 ggactcacta tgttttccgc attgcgccac cgtaccgctg ccctggcgct cggcgtatgc
    28981 tttattctcc ccgtacacgc ctcgtcacct aaacctggcg attttgctaa tactcaggca
    29041 cgacatattg ctactttctt tccgggacgc atgaccggaa ctcctgcaga aatgttatct
    29101 gccgattata ttcgccaaca gtttcagcaa atgggttatc gcagtgatat tcggacattt
    29161 aatagtcggt atatttatac cgcccgcgat aatcgcaaga gctggcataa cgtgacggga
    29221 agtacgatga ttgccgctca tgaaggcaaa gcgccgcagc agatcatcat tatggcgcat
    29281 ctggatactt acgccccgct gagcgatgct gacgccgatg ccaatctcgg cgggcttacg
    29341 ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca tgctggaatt ggcagaacgc
    29401 ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg cgaccagcgg cgaagaggaa
    29461 gggaaattag gcgcagagaa tttactcaag cggatgagtg acaccgaaaa gaaaaatacg
    29521 ctgctggtga ttaatctcga taacttaatt gttggcgata aattgtattt caacagcggt
    29581 gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca gggcgctggc aattgcgcgt
    29641 agtcatggaa ttgccgcaac gaccaatccg ggtttgaata aaaattatcc gaaaggcact
    29701 gggtgttgta atgacgcaga aatattcgac aaagcgggca ttgctgtact ttcggtggaa
    29761 gcgactaact ggaatcttgg gaataaggat ggttatcagc aacgcgcaaa aacacctgcc
    29821 ttcccggcgg gaaatagctg gcatgacgta agactggata atcaacaaca tattgataag
    29881 gcacttcctg gaagaataga acgtcgctgc cgtgatgtta tgcggataat gctaccgctg
    29941 gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat gggagctggg agttctaccg
    30001 caagggcggg gaattctaag tgatatccat catcgcatcc agtgcgcccg gtttatcccc
    30061 gctggcgcgg ggaacacacg cgcgtaccgg atcgcggaca acaaattgcc ggtttatccc
    30121 cgctggcgcg gggaacacct gaaaccagtt ggctcgttgt tcttctttgg cggtttatcc
    30181 ccgctggcgc ggggaacacg ctggttttga atcgtcccgc gctcgagacg ccggtttatc
    30241 cccgctggcg cggggaacac ggcaacataa cgaacaaaat caacgtcaac ctcggtttat
    30301 ccccgctggc gcggggaaca cttgctgaaa aagaaggctc cggcgttatc agtcggttta
    30361 tccccgctgg cgcggggaac actagttaac ttttagacag aatatccgtg tacccggttt
    30421 atccccgctg gcgcggggaa cacggtttta caagcttaaa tgattttaaa ttttgcggtt
    30481 tatccccgct ggcgcgggga acacaactgg attctgctct aattattaca tcatcgcggt
    30541 ttatccccgc tggcgcgggg aacacggaaa ccgccccggc ggtggttagc tgtttgccgg
    30601 tttatccccg ctggcgcggg gaacactcgg ccagcgtcca ggctggcacc cgatattccg
    30661 gtttatcccc gctggcgcgg ggaacacctg ccgactgacc gccgacgctt tcgcgttaac
    30721 ggtttatccc cgctggcgcg gggaacaccg tttttgccgg gtttatttta aaaccggagc
    30781 cggtttatcc ccgctggcgc ggggaacacg gaatatcgtt gcgctaaaag agattctggg
    30841 ccggtttatc cccgctggcg cggggaacac attaaatttt gcaggtagct gttatccgtg
    30901 tccggtttat ccccgctggc gcggggaaca ccgattcctc gtagctgcca ctcgactgaa
    30961 agccggttta tccccgctgg cgcggggaac acgtcgcctg ttcaacttta aacggctcaa
    31021 accacggttt atccccgctg gcgcggggaa caccctggtg gagattgacg cgaaatatga
    31081 gaaaccggtt tatccccgct ggcgcgggga acacatccac atttccgccg ttatgaatcg
    31141 gtattccggt ttatccccgc tggcgcgggg aacaccccgt gccagtttgc tgcgctgccg
    31201 tcgtcgtcgg tttatccccg ctggcgcggg gaacacgctg aggctgtcag gagccagtgc
    31261 ggcagaggcg gtttatcccc gctggcgcgg ggaacacgag ccgggtcacg cgaactactg
    31321 gcgcgggcgc ggtttatccc cgctggcgcg gggaacacac taagcataca tatctgtttt
    31381 taaacaaatt tattccacat caacaatcta ccaactaaat tcaaacattt ccttattttt
    31441 aaagaacgtc taacttattg attatcaaca ggaagaaaag aaaccaaacg taacccatcc
    31501 aaatccaccg gaatacgtct gtttttgccc caggtctgaa actcaaaacc agactcagta
    31561 tttgtcgccc aggccatgac cacatttcca caacctgcca gttgggtaat ttgctgccag
    31621 atcatctccc gaatacgttt tgatgtatca ccaacataca caccggcacg cacttccagt
    31681 agccagattg cgagccgtcc acgtaagcgc ggcgggacat tttctgtaac aaccacgacc
    31741 atgctcatca gccgcgtcct cgatgaccgc tatcgcccaa cgtttcaggt tcggggatgg
    31801 ctggcggtaa catatccggc gcaggttgtg gcggttcaat ttcacctgcg gcaaggactt
    31861 cctcaattaa cggtattaat ttgcccgtta acttagtgct acggaaaata tcacgacagg
    31921 ctaatctgac ttctttatca ggttctgcgg gttgcctcgc cgctatttca aatgcctttg
    31981 gtacaaccga atcaaattta atgatatcgg ctatgtcata aacaaatgaa agcggtttgc
    32041 cactatggat aaacccaata gcgggcgcat atcccgcggc taatactgcc gcttcagata
    32101 taccgtacag acatgatgta gcagcactga tgcagcgatt cacaacatcg cctttttccc
    32161 agtctttagg atcgtatttg cgaccattcc atttcacacc atattgtttc gccagtaatg
    32221 catacgtctg gcgaacgcga gatccctcaa ttccacgtag ctgctccact gaacggcgag
    32281 ctggcggtgg ctcacgaaaa cgtaattcat acattttgcg caccaccttc aggcgtagat
    32341 cttccgttaa agccagcttt gcctggtaga gtaacttatc agcacgcgcc ccaccgggtt
    32401 gtccggaaga gtagacgcga acacccgctt caccgaccca gaccagtagc gtccccaccg
    32461 tggcggccag atgcaccgcc gcgtgggaaa ctctcgttcc cggttcgagc ataatgcagg
    32521 cgaccgatcc caccggaatg tgcgtgcgga tcccggtttt gttgatcagc acgaaagcac
    32581 cgtccagtac gtcgatttga ccgtactgga ggaagatcat ggaggtgcga tcttttaacg
    32641 ggatcggact cagtggtaca aacgtcacgc ctctgctccg ggtttgatca gcatcagacc
    32701 acagccgaac gcccggcttt tgccataccc ctgactgaga cgttgcagaa ataacccggg
    32761 atcggtgacc gtaagcatcc ccgtataatc cacgctactg aactggatca gttgccgggt
    32821 gttttcccgc cggagttgtt gttgtcggta ggcatcaacc gaggtatcca gtagtgtaaa
    32881 accacttctt tccccctgcg ctgccagcca gtccagcgcc gcctgttgtt gatgcaacca
    32941 gatatcacgc ccttccgcct gccccttcac ctgccgtttc gcctccatca gcagatcgtg
    33001 gcgcttgccc gctttacaga ttgttggatt cgcccgcagg ttgaaacaaa gagattgtcc
    33061 ggtacgcagc tcggacacaa atggtcggca ttcgatggtg aaagtttcac tttccgccgg
    33121 gcgttcctgc gagaggacaa aaaagcgaaa cgcgccctgg agttcttccc gacgataaag
    33181 aaattgcctt tctttgccgc cagggaagag atcccacagc cactgatgca tcacatattc
    33241 accgcgatcc accagatgca gcaactgcgc tggggaaagc tggccggtat gtaaggttat
    33301 tcttgagagg tacatggttc ctccttgctg agccacggcc cctgattgac ggtgcgttcc
    33361 ccaaacaacc actgctgacg atttaaagga acatcccgac ggcgtaatat tttgctcgcg
    33421 accaggccgt cgtgttcccc ttcccaccag cattcatcct gaagttttgg gagtgagact
    33481 ttcagtttgc ggaaatgatc ctgatactgt tggtatgcgt tacgtaaggt atccgacgcg
    33541 ttgccttcga gtagtaacgg cgcaagtggt aacgccagcg gatgactttt tcgccccaga
    33601 taaagcggaa aaaccggatg gcgtaaaccg tcctgcaact gttcgaggct gtaaggcgca
    33661 tcgggggttg ttgccaccgc caccatccac caggcatcgg tgtagtagtc gcgccgggag
    33721 ataatcgcgc tcaaaagctc aggggcgctc aactcttcgc gacggctgaa ataacgtgct
    33781 ttacgcacct cttttggcat ctggaccgtg tgataatccc gtgcccagcg cgggttacgg
    33841 ctggcgcaaa ccaccagtga atagtggcga ttaaacgcgt ttaagcgttc ggtatcatca
    33901 cgccggatcc ctaccccggc agccagcagc cccagcaatg ctgagcgcga aggcagttca
    33961 tgggtatgac gcacttcgcc gggggcatcg acgccccagg atgccatcgg cccatgaagc
    34021 tgaaaaatca gatattggct cataagccgc cccttacgcg cagataaagt ccagcacgtc
    34081 cttcatgctt ccctgtttgt tcatcacgtc aaaacttgcg taatcggtct tctgttcgta
    34141 gaccgtattc atattttcgc gaagagttgt aatacgctgc accgccacct ctaactgacg
    34201 ggtgccatta atgggttcat agaaagctgc cgccagagaa cgcggttgat cggtgccttt
    34261 ttctgccagc gcccaggagg cgtaggcacg gctggcaaag ctgttctgtt tgccggttgg
    34321 ggagacttta agtgcggctt ccgtaaaggc acgtatggtc tgattagcta acgcttcgtc
    34381 accgccaagg ttttcgacca gcagatcttt atcaatgcag atataggtgt agaacagtgc
    34441 agaaccgaat ccggtttcac caagatgccc ggcaccggca tcttcagaag cctggcgcaa
    34501 atcatcaacg gcggtgaaaa aatcatcttc gacaatcgtt tcactgacac caaatgcgtg
    34561 cgctacctgg caggcggctt caacattaaa ctcgggttta ttcgccagca tacgaccaaa
    34621 catggcaata tctaccgcca tacggtcttt gcgtaacaac gcgagatctt cctcttttgg
    34681 cgcgcgcttt tcttctgcaa gctgatgggc cagcgctttt acggcgtcaa attctgccgg
    34741 gctgatatgg actaactgtt cagtttcggc gttagtgagc ggatcttttg gttttttgtc
    34801 gtttttagct ttcccaagat aatctgcaat ttttgccgcc cattcgatgg cttttttctc
    34861 ttcgattcct ttctcaatca ggatagttgc cgcctcacgc gcaatacgcc cactgcgaat
    34921 accaatatgg cccgccagtg cctgttcaaa aagtgcagaa gtgcgccacg cacgtttcag
    34981 actttgcgag gaaacgcgca gacgcgttgc tccacccagc accacagttt ttggcgcacc
    35041 ggtatcatca cggttaaggt tggcagcagg gtaagcggtt aacaaatgaa gctgaataaa
    35101 tgttgtcatg agatagtcct ttattgattt tgttcgttat cggcgtcgcc agcctggtaa
    35161 tattccagcg cccagcggat acggataaat tcggttggcc gctgctggcg gcggtgatga
    35221 ttgagcaggt cgtcgctctc ctggcaccaa cggaagaccc cctctgccag agagtcaaga
    35281 ttgacggaac cgtttaataa tctgactgcg cggcgtaact ggcgcagtaa ttcatccggt
    35341 gtttttactg ccgacagacg agtaaaacgc ccctttgaca ttaccgccgc cagctgcgcg
    35401 gcaaaaggct gccgttcatc aatagcttta acattggcgc taactgcggc tatcagcgcc
    35461 agtgcggtga tacgccactc aggttcatcc tgccacttta tttgtctgtt cttcagaaac
    35521 aggcgaaatc catccgtcag acaaacatca ttaaccgtcg tactacgccg cagactggca
    35581 cgttcgccac gtttctcctg caattcctcg tgccatttgc gcagcgtggc tttgtgctcc
    35641 tcttttacaa tactcattca gcagcctcct gctttttctc cctggcggct ttagcacttt
    35701 gcttctccgc cgatgttgtg aaatatttct tgcgcgcggt cataacgcgt ttcaaatcaa
    35761 caggctcata tggattggtg aatacacgct cgtcaaaatc ctgacgcgcg aataaccaaa
    35821 tttccttttg ccatttgccg agtaattcgt ccgcatcctg accttcttca atttggcgca
    35881 ctaacctcag gaagcgatgc tgagttttgt tccagaagtc gatatccaca aaactgaaat
    35941 caccccgtgc accttttgga tcggagaacc acgcttcttt caatgcactt cgtaacagac
    36001 ttagaatccg tgaagccgtt tgcgcagcca gacgcagttt cggtatctgg ccttcttttt
    36061 tcttgagcag cagcgggaaa tggtgttcgt accaacagcg cgctttcatg ttgtcgaaat
    36121 catagccaaa tccccacagg cctacttttg cctgtttcag actgctggca ttaaagagtt
    36181 tcaccaccag cgcgggaagt tccgtattgt tttctgattt acccgtttcg ataaggccta
    36241 accagtcgcg ccagattaaa ccgcccggtt gtggtttgac cgagtaaaac tcgccaccct
    36301 ctttaagtgg tatacggtaa ggcgttaagg gatgctgcca catggcataa ttcgcaccgt
    36361 aatttttggt cgtcatcagg ctcaggagcg cgtcgctctg ttcaccgcaa atatcgcagt
    36421 tgccgactgt cgtggtatta aaatcaatac gaatacggcg cggcattccc cagtaagcct
    36481 ggagtttatt gacctgatca tgggttacca ccgcaccggc cagttcgctg gttcgcgtcg
    36541 ggccaagcca ggggaaaacc agatcgtcaa attttttggg tagcggtaag tcggcttcat
    36601 cctgcggcat cacattgggc cacagtttgc gccacaaggg cgtttgttga ttgccttgat
    36661 attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc gcgcaaaccg gtgcgatagc
    36721 ctttgccgcc tgatggcgca tttaactgta gggagaacag agctaacgcg gaacaatgag
    36781 ggcatacgtg ttcagtcacg ccacgcttaa taaagtggtc tttattaaac ttcgttgttt
    36841 gagcgccggg aatctcaggc agtagcgaag cgacctgaac tttatctccc ttgagcgcct
    36901 cgaaatcctg cataaatgaa ggggaatctg gcccaaactg gaaagcatgt tctaatgact
    36961 gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc ccagatatca tcccaacgac
    37021 gatgatcttt tggcgcgaag ctggtttgta gtagccccag caaaaactgc cataccgcac
    37081 cctggagatc tgcccgcggc gcagcgatat cgacaacatt ttcatcagcc agatcgactg
    37141 gtgccagctt gcctgtcgtt ccgtctttaa aacgaacagg caaccacggg gttgtcagaa
    37201 gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc atcccaggtc ggttgtccat
    37261 ccttccatcc ttttctgtgt atcaactatt cagagagtcc tgtcgcagac atctcaacca
    37321 accttgccaa tcaatccttc cgtagccgaa tacccgcaac tttcgtcatc gttgactaaa
    37381 atcacgtttg ccatctccgg atcttgtcgc tgttcaatgc accacagcct gaacgcctct
    37441 ccctccagta aagaaaactc atcccgatgt tttttccacc agcttcggcg cactctgaca
    37501 acgctcattt cccatgcgtg agcaccggtg gcataaggct tcaccacacc gtcaatacag
    37561 gtagccagcc acagggaaac agactcctca gccagacgtg tcgacagttt ttccgggagg
    37621 taatcgttga tattggcggc atagccgggc ctgaagttca gcacaaactt tttagccatt
    37681 gagcgatcgc agtaatattt gcccacttgc tcctgctcgc tgcgggcaaa tccttccggc
    37741 attaccacgt cctcaccgta gacggattca ataagaagcc gggctgcgtg tggcatctga
    37801 atagcacctt gctcacgcag cacacgctgc gtcagccaga ttcgcccatg atcgggatag
    37861 acaaatgcac tgttacgcat ggcactgcca aaccattcgt cgccaggaga gtcgtcccag
    37921 acgggagcca gaatcagcaa ttcagggggg gaacgctcgt cctttccgtc acgctttaac
    37981 tgaccattaa tatcgcggat atgccgctgt aatcgtcctg ctcgctgaat cagcaaatca
    38041 acaggggcca ggtcggagat catttcgtcc aggtcaatat ccacgctttg ctcaattact
    38101 tgtgtacaaa caatgacttg cgacgctctc tgtaaactac aatatttacc aaagcgtgcc
    38161 agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa agcggctatg aaaaagtgaa
    38221 aggctggaag caggaatgac gccgcgggca agcagctgac gatgaacctt aatagcgtca
    38281 tcgacagaat tccggatcca ggcgatgcat ttcccctgac ttaccgccga ttcgatacgc
    38341 gcaatacact cttgttcact atgaagccaa cccacgctga cgctacgctc aacgtctttg
    38401 cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga catgcgtcag ccaggggtaa
    38461 tcatcctttt ccaggaacgg agcttcttgc tggccctctg taccccgcgc aaaggcagcg
    38521 acgagtttgt cgcgctgctg ttgagataac gtggcagaaa gcaaaataac gctgtttccg
    38581 ccacgcgcct gccgctcgat caacccttca agaatgcacg acatgtaagc gtcacaggcg
    38641 tggatctcat cagccagcag gattttgtta ctcagcccca gaagccgcag attattatgt
    38701 ttgaacggca tcaccgccat catcgcctga tccagcgtgc cgacgccaat ttcagccagc
    38761 aacgccttct tgttactgtc ggcaaaccag gccgcacatc cctgactgaa cgtttgttca
    38821 tccggttctt ctgacccgac taaatcacca gaccagagtg attcattaaa gcggtccatt
    38881 aatgtgcggg cactatgtgc cagcaccagg ctggggcggg attctggcga atagaaagca
    38941 agccaggttt tgaccagccg atcgtacatg gcattggccg ttgccattgt tggcagacca
    39001 aaaaacagcc cctgagcttt ccccgcagct atcaacctgt gcgccaggat aagcgccgct
    39061 tctgttttac ctgcgccagt cacatcttcc agaataaata actgcggtcc ttgctggctg
    39121 atatccagat ccagcacctt ttgctgtaac ggtgtcggat tctcaataaa aggaaacagc
    39181 gtattaattc cgttgaaagg cgcgactttt gcttttagag gaaagacggt taaggcgttt
    39241 tgagcctgaa ttaaagtttt ctgccagtaa cctttaatat ccattgggtg tgcgacgcgt
    39301 ggaaaaaatc gcgttgacga acccgtccag tctgcgagca cgacggttgc agagatatac
    39361 caggaaaggt gttttataag ttcaatgccc tcatcatcat cccagaatgc ggggatctct
    39421 atgagcggaa acagtggttt gatttcaagg agaaaatctc gtgctgcagc tttgtcttca
    39481 ggcagaaaat tatccaattc atccatacga tcagggggct gaccatgatg ccctgtcgtc
    39541 acgggcatcc acatttctat cactcgccta agtttgcgcg gagagagcga agatgaagga
    39601 agtaactcct gacattcact taaataatga ttccacaacc agtagcccag cgttgaatga
    39661 gagatttttt cgtaattttt tctggcacct tcctgaatct tgagttcagg gggcaggtaa
    39721 agttgttgaa aagagcgggc aaattttcca atatcgtgcc agcacagcaa ccaggcgaaa
    39781 aattgagccg cctgttcctt gtcagaaatc cctaattgac gaaaataatc agccagcccg
    39841 aagcaatttc ttttaaccat taaatagccc atcgcggcta catccagcga atgccagcaa
    39901 agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc gggtttttcc ccagaaatca
    39961 aaaaaagtca caatattttt atccttcagt aaacttaaag gatat
//
LOCUS       DAPPNJ010000015        39642 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000015 DAPPNJ010000000
VERSION     DAPPNJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.015"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(142..999)
                     /gene="murI"
                     /locus_tag="RPM73_000598"
     CDS             complement(142..999)
                     /gene="murI"
                     /locus_tag="RPM73_000598"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDX6518992.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(944..2788)
                     /gene="btuB"
                     /locus_tag="RPM73_000599"
     CDS             complement(944..2788)
                     /gene="btuB"
                     /locus_tag="RPM73_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDX6518993.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2853..3043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3157..4257
                     /gene="trmA"
                     /locus_tag="RPM73_000600"
     CDS             3157..4257
                     /gene="trmA"
                     /locus_tag="RPM73_000600"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDX6518994.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAESLRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4297..4656)
                     /locus_tag="RPM73_000601"
     CDS             complement(4297..4656)
                     /locus_tag="RPM73_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDX6518995.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="RPM73_000602"
     CDS             complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="RPM73_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDX6518996.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5637..7037
                     /gene="sthA"
                     /locus_tag="RPM73_000603"
     CDS             5637..7037
                     /gene="sthA"
                     /locus_tag="RPM73_000603"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDX6518997.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7020..7937)
                     /gene="oxyR"
                     /locus_tag="RPM73_000604"
     CDS             complement(7020..7937)
                     /gene="oxyR"
                     /locus_tag="RPM73_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDX6518998.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8203..9576)
                     /gene="argH"
                     /locus_tag="RPM73_000605"
     CDS             complement(8203..9576)
                     /gene="argH"
                     /locus_tag="RPM73_000605"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDX6518999.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9637..10413)
                     /gene="argB"
                     /locus_tag="RPM73_000606"
     CDS             complement(9637..10413)
                     /gene="argB"
                     /locus_tag="RPM73_000606"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDX6519000.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10421..11425)
                     /gene="argC"
                     /locus_tag="RPM73_000607"
     CDS             complement(10421..11425)
                     /gene="argC"
                     /locus_tag="RPM73_000607"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDX6519001.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPMINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11579..12730
                     /gene="argE"
                     /locus_tag="RPM73_000608"
     CDS             11579..12730
                     /gene="argE"
                     /locus_tag="RPM73_000608"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDX6519002.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            13082..15733
                     /gene="ppc"
                     /locus_tag="RPM73_000609"
     CDS             13082..15733
                     /gene="ppc"
                     /locus_tag="RPM73_000609"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDX6519003.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15916..17649
                     /locus_tag="RPM73_000610"
     CDS             15916..17649
                     /locus_tag="RPM73_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDX6519004.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17798..18649
                     /locus_tag="RPM73_000611"
     CDS             17798..18649
                     /locus_tag="RPM73_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX6519005.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18636..18977)
                     /locus_tag="RPM73_000612"
     CDS             complement(18636..18977)
                     /locus_tag="RPM73_000612"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDX6519006.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18979..19857)
                     /locus_tag="RPM73_000613"
     CDS             complement(18979..19857)
                     /locus_tag="RPM73_000613"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDX6519007.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19823..22120)
                     /locus_tag="RPM73_000614"
     CDS             complement(19823..22120)
                     /locus_tag="RPM73_000614"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDX6519008.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAELTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22171..22491)
                     /locus_tag="RPM73_000615"
     CDS             complement(22171..22491)
                     /locus_tag="RPM73_000615"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDX6519009.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22506..23585)
                     /locus_tag="RPM73_000616"
     CDS             complement(22506..23585)
                     /locus_tag="RPM73_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDX6519010.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23894..26395
                     /gene="ptsP"
                     /locus_tag="RPM73_000617"
     CDS             23894..26395
                     /gene="ptsP"
                     /locus_tag="RPM73_000617"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX6519011.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26407..27069
                     /gene="fsa"
                     /locus_tag="RPM73_000618"
     CDS             26407..27069
                     /gene="fsa"
                     /locus_tag="RPM73_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDX6519012.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27080..28183
                     /gene="gldA"
                     /locus_tag="RPM73_000619"
     CDS             27080..28183
                     /gene="gldA"
                     /locus_tag="RPM73_000619"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDX6519013.1"
                     /translation="MDHIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28458..29075
                     /locus_tag="RPM73_000620"
     CDS             28458..29075
                     /locus_tag="RPM73_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDX6519014.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29102..30007)
                     /gene="yijE"
                     /locus_tag="RPM73_000621"
     CDS             complement(29102..30007)
                     /gene="yijE"
                     /locus_tag="RPM73_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDX6519015.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30101..32281)
                     /gene="katG"
                     /locus_tag="RPM73_000622"
     CDS             complement(30101..32281)
                     /gene="katG"
                     /locus_tag="RPM73_000622"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDX6519016.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32610..33500)
                     /gene="metF"
                     /locus_tag="RPM73_000623"
     CDS             complement(32610..33500)
                     /gene="metF"
                     /locus_tag="RPM73_000623"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDX6519017.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEIYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33849..36281)
                     /gene="metL"
                     /locus_tag="RPM73_000624"
     CDS             complement(33849..36281)
                     /gene="metL"
                     /locus_tag="RPM73_000624"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDX6519018.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36284..37444)
                     /gene="metB"
                     /locus_tag="RPM73_000625"
     CDS             complement(36284..37444)
                     /gene="metB"
                     /locus_tag="RPM73_000625"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDX6519019.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37721..38038
                     /gene="metJ"
                     /locus_tag="RPM73_000626"
     CDS             37721..38038
                     /gene="metJ"
                     /locus_tag="RPM73_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDX6519020.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38098..38705
                     /locus_tag="RPM73_000627"
                     /pseudo
     CDS             38098..38705
                     /locus_tag="RPM73_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
     gene            38850..39525
                     /locus_tag="RPM73_000628"
                     /pseudo
     CDS             38850..39525
                     /locus_tag="RPM73_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9675 a        10969 c         9988 g         9010 t
ORIGIN      
        1 cggagttctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttctg
      121 accgcgcaac attcaaccaa atcagcctaa aactgccagt ttttcgagcg tttcgaagcc
      181 gtaacgctgt aaaacgggca ataattgttc agcttctggc gtcattgcca tacaaaaggc
      241 aatattcgca tcggcagatt ttgcatccgg agcttcatgt tctaacagcc aggccgttct
      301 gcgagcaatc gctgcgccag aatccaccag ccgggttccc tctggcagca cctgtaagag
      361 ttcttcttgt agtagaggga aatgggtgca acccaataca acggtatctg gcggctcttt
      421 cattcttaac cacgggcgta ggatacgttt tagtgcatcc agagaaacat cttcgccatg
      481 cagcttcgct tcagccaact caaccatctc tgccgagccc agcatttcta tctggcattc
      541 attagcgaaa cgcgcgatca gctcatgagt ataagaacgt ttaactgttc cgcgggttgc
      601 cagtaatccg acaatgccat ttgccgtcag acgtgcagca ggtttaatcg ccggcacgac
      661 accaacaacc gggaagtcga acttttcgcg taatgcagga agtgaaacgg tactggcagt
      721 gttgcaagcg accacagcca gcgcaagggg ataacgttct tgcaccgcgg tgacaattgc
      781 caccactcgc tcaacaataa acgcttcgct tttttcgcca tacgggaaag cgacgttatc
      841 gaaagcataa atgtaatgga gatccggtaa gagatgccgg atctcgtcat agaccgacaa
      901 cccaccgacg ccggagtcaa acaccagcac ggtgggacgt ggttcagaag gtgtagctgc
      961 cagacaaggt gtattcccgt cctgcagttt ggtagccata gactgtctca taatctttgt
     1021 cgaacaggtt ggctatttta ccacgaactg tcaggtgaga ggtgaccgga tacgcaaccg
     1081 caagatccca caagctcaca ccgcccattt taacggtttg ataaggataa gatgagtaat
     1141 ccttatcata gcgagtgcct aaatactgat aagtaatacc ccagtcgaag tcatacaact
     1201 gccagtcgag ctggtatttc acctgctgtt tagcacggcg taacaacggc gtgtcggtaa
     1261 tcgcattgcg cgcatcgaca taatcataac tcacagtatg cgtcagtggt ccggtatcaa
     1321 aattggcggt cgcctcgacg cccttaatcc gcgctttccc ttcgttgtaa tatttcaggg
     1381 tgtgatcatc ataatcgatc aagtcactga catcgttacg atatccggaa atacgccagt
     1441 tcaccccagc ggttaagcct tcaaacgcgc cttcccactg tttgcttttc tccgggtcca
     1501 gattcggatt tccgtagaag ccatacagtt gccccagatt tggtgcctta taagatgtcc
     1561 cgtaggaagc aatgaagcga taaccttcga tgaattccca accggcgctg gtttgccagg
     1621 ttccatgacg accaaactgt gagttatcgt cactgcgtgc tgcgccttca aaggtaaaat
     1681 cgccgacttg ttgcagcccg gtcagataga tgccggtatt acgttgatca tatccatcct
     1741 caacataacc tgtacccggc gtcgtagtct gtttctgcca gtcgacaccc gcaccaatac
     1801 taccgtgacc aacgatgaca ttgtttgccc actggacggt gtattgcttc atctcatcga
     1861 gcgtcgccga cgaatcataa cgaccataat ggggatcgta gttgtaatct ttgctatggc
     1921 tatagctggt aatgagttgt gatttaatca gttcgccgtt atagcgcagc ccggcgtccc
     1981 aactttggct atagagttta cgggtatcga gcaacggtga accgggagaa taatacgcgt
     2041 cataattggt acggttatca tagccatagc cgcgcacaaa gccgctccag gcatcagtaa
     2101 agttatgctc cagcgcgcca taaagcgttt tacttaaaaa accatcgtta tctgtctgcg
     2161 cttgcgttcc ggtattacca taggcaacaa catcataacc atgagtatgg gcataatcgc
     2221 ccaacagcgt tacccgtgtc ttatccccca gttgttgctg cgtagagaca tcatagttct
     2281 gataactatt gcttccccac cctgctgaaa tttccgttcc gggttcatcg cgcgtcgtga
     2341 tgatattcac caccccgcct attgcatcgg aaccataaac agcggagcgc ggcccacgga
     2401 tatattcaac acgctggaca agcgcaatag ggaactggct aaggtcggca gaaccactca
     2461 cccccgccag attcaggcgt acgccatcaa ttaacaccaa cacatgactg gcatttgtac
     2521 cgcgaataaa aatagatgag agctgacctg aaccgccgtt ttgggtgata tcgacgcccg
     2581 gaagacggcg cagcacatca ttgaccgagg tcgactgcca gcggtcgata tcctgacggg
     2641 tcacaacggt ggttggtgca agcacagtgc tgcgcggctg ttcaaaacgg ttagcagtaa
     2701 cgacgagagt atccgggctg gtatcctgtg cccaagcgga aaaggctgtg acagaacacg
     2761 ccgtcagcag cgaagctttt ttaatcattg taaagcatcc acaatagaag aaggatgccg
     2821 caggtttcat caatattacg cgatgatgag aaccagatgc gacgttggcc ggcaggtctt
     2881 cgggcttgga ggggtatcta agatactaag agatgatgac ttcccaccgg atggcagtgt
     2941 ccgcataacg caatcatcgc acctttcctt accgctgcgc gtcagctcca gattcgcact
     3001 ggattcccta ttaactcaca ggaccggcaa gtggatgcta caggttgtaa caagttactg
     3061 tccagacgta gctcacaaat aggaattcat caagatctgg acatctgatg agcaatccct
     3121 acaatcgccg cgtactttaa tttttcagga tacatcatga cccccgaaca ccttccaaca
     3181 gaacagtatg aagcgcagtt agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg
     3241 ttttctgacc tggttccgga agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg
     3301 gagttccgca tctggcacga tggcgatgac ctgtatcaca tcattttcga tcaacaaacc
     3361 aaaagccgta tccgcgtgga tagcttcccc gccgccagtg aacttatcaa ccagttgatg
     3421 acggcgatga ttgcgggtgt gcgtaataat cccgttctgc gccacaagtt gttccagatt
     3481 gattacctca ccacgctgag taatcaggcg gtggtttccc tgctgtacca taaaaagctg
     3541 gatgatgagt ggcgtcagga agcggaatcc ctgcgcgatg cactgcgagc acagaatctg
     3601 aatgtgcatc tgattggtcg ggcaacgaaa accaaaatcg agctggatca agattacatc
     3661 gatgaacgtc tgccggtcgc agggaaagag atgatctacc gtcaggtaga aaacagtttt
     3721 acccagccga acgcggcgat gaatattcag atgctggaat gggcgctgga cgtaaccaaa
     3781 ggctcaaaag gcgatttact ggagctgtac tgcggcaacg gtaacttttc attagcactg
     3841 gcgcgcaatt ttgatcgggt attagccacc gaaatcgcta agccgtcggt tgctgctgcg
     3901 caatacaaca tcgcagctaa ccatattgat aacgtacaaa ttattcgtat ggcggcagaa
     3961 gaatttactc aggcgatgaa tggtgtacgc gagtttaatc gcctgcaagg gatagacttg
     4021 aagagttatc agtgcgaaac catttttgtc gacccaccgc gcagcggtct ggacagtgaa
     4081 accgagaaaa tggtgcaggc gtatccacgt attctgtata tctcctgcaa tccggaaacg
     4141 ttatgcaaga atctggaaac attaagccag acgcacaagg tcgaacgtct ggctctgttt
     4201 gatcagttcc cctacacgca ccatatggag tgcggcgtat tactgaccgc gaagtaaaac
     4261 ccatgccgga tgcgccagca tccggcataa taccgattac tcagcaactt cctgcttacg
     4321 gttacgcatc ttcgcgccaa tccagaacac cataatgacg gagagtactg ccgggaagaa
     4381 gttagagccg atatccggat attccgcacg aactaccgtg ctgtacagca gtacgccgag
     4441 aataaaacag gcagctgcca gacccggcaa gcctaccggc atggtgcgat taagataacg
     4501 ttgatgcagg cagtaaaccg tcagcaccag ggaaataatc gggaatacag aaaatggcac
     4561 aatggagcta aacagcgctg cgaaagtacc attaatcgat aagccagcaa ccaacgccag
     4621 cagcagcgta cctctatctt gatttgcttg tttcattact cgtccttcac atttcccgga
     4681 ataattgcgg ttttctcttg ttcacggcga taccagtaat aagccccttt cgaaatcatt
     4741 cgcagttgca gtaccagtcg ctcttctaat tgccgacgtt gttcgacgcc gacgtccaac
     4801 gcctcggcac ccgcactgaa gacaattgtc accattgctt cggcttgcgc ttcagtaaac
     4861 gcacgcggca tatggttttc gagttccaga tagtccgcaa gttccgcaat gaagtgctga
     4921 atttcacgcg caacggcggc acgaaacgca gcggaggtgc cggagcgttc ccgcaataat
     4981 aaccggaagg cgttaggatt attaccgatg aactccataa atgtggagac cgaggtgcgg
     5041 atcacactcc cgcctttggc gatacgctga cgcgcctggc gcatgagttg gcgtagcatt
     5101 aaaccgctct catcaaccat ggtcagaccc agttcgtcta cgtcgcggaa atgccgataa
     5161 aaagaggtgg gagcaatgcc cgcttcacgc gccacttcac gcaaactcag gctggcgaag
     5221 ctgcgttcag cacttaattg gctaaatgcg gcttccacca gcgaacggcg ggttttttct
     5281 ttttgttgcg ctcttacgcc catcacgatg tctgaatcct tgccaaaagt actgctggca
     5341 ctataccaga gaatgaacat actggattgc tccagtattg tgaatgaacg gtaacgcaat
     5401 aaaacatatt tactgcaatt ctttggccgg ttcttttacg tacagcggaa acctgccgct
     5461 taaacggaga gtatcgtcga taaaaatccc ataaaacgtc agggcaaaag taagaaacag
     5521 acaaagcaaa ggccgctcag gatatagcca gataaatgac ggggatcaat tggcttaccc
     5581 gcgataaaat gttaccattc tgttgctttt atgtataaga acaggtaagc cctaccatgc
     5641 cacattccta cgattacgat gccatagtaa taggttccgg ccccggcggc gaaggcgctg
     5701 caatgggcct ggttaagcaa ggtgcgcgcg tcgcagttat cgagcgttat caaaatgttg
     5761 gcggcggttg cacccactgg ggcaccatcc cgtcgaaagc tctccgtcac gccgtcagcc
     5821 gcattataga attcaatcaa aacccacttt acagcgacca ttcccgactg ctccgctctt
     5881 cttttgccga tatccttaac catgccgata acgtgattaa tcaacaaacg cgcatgcgtc
     5941 agggatttta cgaacgtaat cactgtgaaa tattgcaggg aaacgctcgc tttgttgacg
     6001 agcatacgtt ggcgctggat tgcccggacg gcagcgttga aacactaacc gctgaaaaat
     6061 ttgttattgc ctgcggctct cgtccatatc atccaacaga tgttgatttc acccatccac
     6121 gcatttacga cagcgactca attcttagca tgcaccacga accgcgccat gtacttatct
     6181 atggtgctgg agtgatcggc tgtgaatatg cgtcgatctt ccgcggtatg gatgtaaaag
     6241 tggatctgat caacacccgc gatcgcctgc tggcatttct cgatcaagag atgtcagatt
     6301 ctctctccta tcacttctgg aacagtggcg tagtgattcg tcacaacgaa gagtacgaga
     6361 agatcgaagg ctgtgacgac ggtgtgatca tgcatctgaa gtcgggtaaa aaactgaaag
     6421 ctgactgcct gctctatgcc aacggtcgta ccggtaatac cgattcgctg gcgttacaga
     6481 acattgggct ggaaactgac agtcgcggac agctgaaggt caacagcatg tatcagaccg
     6541 cacagccgca cgtttacgcg gtgggcgacg tgattggtta tccgagcctg gcatcagcgg
     6601 cctatgacca ggggcgcatt gccgcgcagg cgctggtaaa aggcgaagcc accgcgcatc
     6661 tgattgaaga tatccctacc ggcatttaca ccatcccgga aatcagctct gtgggcaaaa
     6721 ccgaacagca gctgaccgcg atgaaagtgc catatgaagt gggccgcgcc cagtttaaac
     6781 atctggcacg cgcacaaatc gtcggcatga acgtgggcac gctgaaaatt ttgttccatc
     6841 gggaaacaaa agagattctg ggtattcact gctttggcga gcgcgctgcc gaaattattc
     6901 atatcggtca ggcgattatg gaacagaaag gtggcggcaa cactattgag tacttcgtca
     6961 acaccacctt taactacccg accatggcgg aagcctatcg ggtagctgcg ttaaacggtt
     7021 taaaccgcct gttttaacac tttatcgaaa tggccatcca ttcttgctcg gatggcctct
     7081 gccagctgct catagcggct gcgcagcggt gagccaggac gataaaccag gccaatagtg
     7141 cggcgtggtt ccggcttaat gcacggcaga taaacaaccc catcgcgttt gcgctccggc
     7201 ggcacagcca gcgcaggcag taaagtgatc ccgctacctg ccgccaccat gttgcgcaga
     7261 gtttccaggc tggtcgcgcg gaagtgtgta tcttcatccg ccccggcttc aaagcagaaa
     7321 cccatcgcct gatcgcgcaa acagtgaccg tcttccagca tcagcagttt ttcccctgcc
     7381 agatcggcca tcggtacgca ttcgcggttc gcccacgggt gatcttcata gatagccagc
     7441 aacattggct catcaaacaa cggcacttca atgaatgctt cgctctcttt caccagcgcg
     7501 aggatcacgc aatcgagttt gccgctgtcc agttgcgcca gtaactggtg ggtctgtgct
     7561 tcatgcagat acatttccag ctttggaaag gtctggtgca gcatcgggat aatatgcggt
     7621 agcaggtacg gcccgaccgt gggaatcaaa ccaatatgca gcggcccgga catggtctca
     7681 ccctgctggc ttgccatctc tttaaggact ttcacctcac gcagcacggt acgcgcctga
     7741 tccaccagca gcattcccgc ctgggtgaac aacactttac ggctggtccg ctccagcaac
     7801 atcacgccca gctcatcttc cagcttacga atttgcccgc taagcgtcgg ctggctaacg
     7861 tggcaggagt ctgccgcacg ccgaaaatgg cggtgttcag ccaatgccac caggtactca
     7921 agatcacgaa tattcattat ccatcctcca tcgccacgat agttcatggc gataggaaga
     7981 atagcaatga acgattatcc ctatcaagca ttctgactga taattgctca caggaacgga
     8041 gcggcacctc ttttaaccct tgaagtcact gcccgtttcg agagtttctc aactcgaata
     8101 actaaagcca acgtgaactt ttgcggatct ccaggatccg ctttttttgc cataaaaaag
     8161 cccggcgata agccaggctc aaatttatac atataaatgt tcttacccca accgcgcctg
     8221 cgcaaaagca attgcctgcg ccacctgctg cggtgagacg ccgccttttg ccgcacgctt
     8281 gtcgaggcac gattgcagcg acagaatcgg atagacatct tcgtcaatca ccgggctgaa
     8341 tttctgcaac tcgtcgagcg gcagatcttc cagcggtttg ccctgacgaa tggcttccac
     8401 caccgcttca ccaacaatat ggtgcgcctc gcggaacggt acgcctttcg ccaccagata
     8461 atccgccagt tcggtggcgt tggcgtaacc ctgctgagcc gcttcctggc aacgtggacg
     8521 tttcacctga atgccgtcca gcaccagcgc cgccatatgc aggcagtcca gccaggtatc
     8581 gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag ccagcggcaa
     8641 acctttcagc gtcatcatca tgccggttaa cgccccctgc acccgaccgc atttaccgcg
     8701 aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac cggaagtcac
     8761 gcggtcagaa agctccacaa accccgcttc gccggtgtta aagaaaatca gatcttcagc
     8821 aaaacgcgac agatgcacca tgccgatagc ggcagccgac agcagttcca acacatggtc
     8881 acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca gccagcctgc
     8941 taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc ctaatgggct
     9001 gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca gcatctcaac
     9061 ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt gagtgtaacc
     9121 tggcatgacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt gcagctgtcg
     9181 gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt cagtcgctac
     9241 ctgatcgtta cgactacgcc cggtatgcag ctttttgcct aactggccca ctttgtcgat
     9301 cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga tttgttgtgg
     9361 cctggcgcga acatcttcca gcagcacgtt cagcgcctct tccagttgcg cctgctcttc
     9421 tgcggttaac acgcctaccg tgaccagggc tttggaccag gccacagagc caacaatatc
     9481 ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga accgttgatc
     9541 tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt attttgaaat
     9601 tcaatgccgg aaagctgcgc ctccggccaa caaaacttaa gctaaaatcc gcgtacccat
     9661 cggcataccg ttaaacagtg ccggaagctg ctccgcatga cgccaggagg cgatatctac
     9721 cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta tcatgccgtc
     9781 agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt cggcaatgcg
     9841 ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat ccgcgcccag
     9901 cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt gcccttcgtc
     9961 tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg agttgataag
    10021 cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa gctgggtcac
    10081 tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat gtttcttcgc
    10141 ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa tgtctatctg
    10201 atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca gccctttcat
    10261 cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct gatgtgactc
    10321 acgataattc accagtgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
    10381 gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
    10441 ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg cctttcagta
    10501 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac ccgatatcgc
    10561 aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
    10621 gcggtttatg ggcatacgcc tgctgtaaca cttgcgcgac ttgcgcctgg gtcacacccg
    10681 atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgtgggg
    10741 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgagtaaaga
    10801 cgccatacgg ttgcaggcta acttcacaaa agctgtttga aatggccgct ttacgccctg
    10861 caccgctcac gccgctggtg gcgttgatca tcggccactg attgaggtca agaagatcgg
    10921 catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagccc ggcaccgcaa
    10981 tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
    11041 ccaacagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gtggcgtcgt
    11101 taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga aactgcggcg
    11161 ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc cctgggctaa
    11221 actcgctgat atccgacatc ggctgcaacg gcagatcaac gatgcctttt agctgcggat
    11281 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
    11341 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
    11401 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
    11461 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
    11521 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
    11581 gaaaaacaaa ttaccgccat ttatcgagat ttaccgtgct ctgattgcca caccttcaat
    11641 aagcgccacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
    11701 ctggtttaaa gatttaggct tcaatgtgga agtgcagcct gtgccaggaa ctcgcaacaa
    11761 atttaatatg ctggccagtt gcggacaggg ggctggcggc ttgttgctgg cggggcatac
    11821 cgatacggta ccatttgatg acggtcgctg gacgcgtgat ccgtttacac tgacggagca
    11881 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
    11941 tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca ttctggcgac
    12001 tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
    12061 cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg cacataaagg
    12121 tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg atccagcacg
    12181 cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc aattgcgcga
    12241 taacctgaaa gaacgttatc actacgaagc gtttaccgtg ccatacccta cgctcaacct
    12301 cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg agttgcatat
    12361 ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc tcaacgatgc
    12421 attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc tgcatccgcc
    12481 gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg agaaattgct
    12541 cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc aaacgttatg
    12601 cccgacgctg gtgttggggc ctggctcaat taatcaggct catcaacctg atgaatatct
    12661 ggaaacacgg tttatcaagc ccacccgcga actgataacc caggtaattc accatttttg
    12721 ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcact gttgtcaaac
    12781 tccagtgccg caataatgtc ggatgcgata cttgcgcatc ttatccgacc tacacctttg
    12841 gtgttacttg gggcgatttt ttaacatttc cataagttac gcttatttaa agcgtcgtga
    12901 atttaatgac gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga
    12961 cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg
    13021 cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa
    13081 tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg
    13141 agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa
    13201 gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc tcaccacctt
    13261 acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa
    13321 cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa
    13381 cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga
    13441 caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg ctcaccctac
    13501 cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gcttaaaaca
    13561 gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc gcctgcgcca
    13621 gttaatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga
    13681 tgaagccaaa tggggctttg ccgtagtgga aaacagcctg tggcaaggcg taccaaatta
    13741 cctgcgcgaa ctgaacgaac aactggaaga gaacctcggc tacaaactgc ccgtcgaatt
    13801 tgttccggtc cgttttactt cgtggatggg cggcgaccgc gacggcaacc cgaacgtcac
    13861 tgccgatatc acccgccacg tcctgctact cagccgctgg aaagccaccg atttgttcct
    13921 gaaagatatt caggtgctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct
    13981 ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg
    14041 ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc
    14101 aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta
    14161 ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct
    14221 gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac
    14281 ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag
    14341 ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct
    14401 tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt
    14461 gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc
    14521 gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcgatgcc
    14581 ggttgctccg ctgtttgaaa ccctcgatga tctgaacaac gccaacgatg tcatgaccca
    14641 gctgctcaat attgactggt atcgtggcct gattcagggc aaacagatgg tgatgattgg
    14701 ctattccgac tcagcaaaag atgcgggagt gatggcagct tcctgggcgc aatatcaggc
    14761 acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg
    14821 tcgcggcggt tccattggtc gcggcggcgc acctgctcat gcggcgctgc tgtcacaacc
    14881 gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa
    14941 atatggtctg ccagaaatca ccgtcagcag cctgtcgctg tacaccgggg cgattctgga
    15001 agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact
    15061 gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc
    15121 ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg gttcacgtcc
    15181 ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt ggatttttgc
    15241 ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa
    15301 agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt ggccattctt
    15361 ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt ggctggcgga
    15421 atactatgac caacgcctgg tagacaaagc actgtggccg ttaggtaaag agttacgcaa
    15481 cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc atctaatggc
    15541 cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa
    15601 cgtattgcag gccgagttac tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc
    15661 ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg caggtatgcg
    15721 taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag ggttttctga
    15781 aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt
    15841 ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat atttttttca
    15901 agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt tagctggaaa
    15961 gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca ggccattatt
    16021 tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt cagctcgctg
    16081 tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc agtaatcggc
    16141 gtggtgctat gggcggcctc tctggcggcg ctatgctact acgtcatcta cgggcaggag
    16201 ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc cagcgagtat
    16261 ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac ggcggtggca
    16321 gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg ttatgttgtc
    16381 tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac gtttatcaaa
    16441 aacaagccgt ttgagaaaac gttggataac ctggcctcgc gtatggagcc tgccgcaccg
    16501 tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct aacaaagtta
    16561 ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc gggtaacgaa
    16621 ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat gagtctgtac
    16681 ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga tccgaatctg
    16741 accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct gcaacaggcg
    16801 ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc gctgatgaac
    16861 atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac gatgaccgcc
    16921 cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta catgaaccag
    16981 caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt ccaggatgtg
    17041 ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac gcatatcaaa
    17101 tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga tcatgttccg
    17161 ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc taacctgtat
    17221 aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc gaacggattc
    17281 ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca caaaactcag
    17341 gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct gctgtggacg
    17401 tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga tcgtaaatac
    17461 agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta cgacggttac
    17521 gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg gattggtaac
    17581 ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga ccaggtaggt
    17641 aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga tactgccgca
    17701 atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt aataatgcca
    17761 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
    17821 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagtaa
    17881 cggtccggcg cccgatcttg cgtatcaggt cgattttcca cggctggaaa tagtgctgga
    17941 aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg tgctgtacgt
    18001 tcccgctggt ggctggaatt ttccacaatg gcaagccccc gctactacct ttagcgtgct
    18061 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    18121 tctggcgaag caacacgtcg cccgacgcgg cccacgtata ggttcttttc tgctacaaac
    18181 gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta tcgtagccag
    18241 cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct cacgcagcca
    18301 ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg cgcttacccg
    18361 cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc tgtttcaaaa
    18421 aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    18481 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    18541 cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt cggaatatcg
    18601 ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag atgggtttgc
    18661 tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat
    18721 gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc ggcaagctcg
    18781 atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc taaacgattc
    18841 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa ctggcacaac
    18901 ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca ggcggttact
    18961 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    19021 ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca tcgaccatgt
    19081 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    19141 cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc ggctgagcgt
    19201 gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca ccagcaaacg
    19261 cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc
    19321 cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagtggta atagcttgct
    19381 ggctggcgcg tctccggcag tttcaatggc gcatgacacg ccccacaacc gcagccgctg
    19441 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    19501 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca gcgcgtcaag
    19561 gctgatatcg cggccaatcc gttcaaacgc cccggagggg cattcatccg catcacgcaa
    19621 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    19681 cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa agaccaccgt
    19741 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    19801 atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    19861 ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    19921 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    19981 actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc agtttgcgta
    20041 atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    20101 acagcagcgt gttatcgagc ttactgactg acttcagtac cgctgttggc ccttgtgcgt
    20161 cctggcccag cataggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    20221 ctggcgtcgc gccaaccacc gatcccaacg ggacgtgagc agaaacggta tacgatcccg
    20281 gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag tagtggcgta
    20341 acagttcggc gctgatgtta tccacctcgt cgatatcgtt accgtatttc tcgaagcggt
    20401 taattaagcg agcacggact ttttcgcctt ctggcgttgc aaagttggct tttaataccg
    20461 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    20521 gagaatcgct caggttggcg ataccgatcc cctgtacgcc ggagaagtta taacgcgcgc
    20581 cgccatcggt aatatcgcgg cctttttcca gacaatcgct gataaaagac gagagcagcg
    20641 gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca accatcaggg
    20701 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagctcag
    20761 cattgccttc attttcatac aggcagattt ccatcacttt cagcagatta aacatcgcga
    20821 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    20881 agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    20941 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt ttcatcagga
    21001 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    21061 tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    21121 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    21181 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agtagttctt
    21241 tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc aacatatact
    21301 ggtcgaagcg ccccaacgat agcgaactgg cgttggattc gtattgcaga atgatgttca
    21361 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttcg gcgttatggc
    21421 gggagatctc cgcaatagtc agcagttctt cgcgacgctg ggcatctgtg cagtttgccg
    21481 ccattgtttc cgccagttca gcgtaacgca gaatgtgttt ctgcgaggct tccagcagta
    21541 acagcgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgcg
    21601 ccaccagctc ccccagcccg tgattcagca ggcgtgggta atcaataata atgtgcccct
    21661 ggcctttatc tgtctggttg atgctgaaaa tctgcgtgct ggtcgcggct tttacttcat
    21721 ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    21781 cttcacggta gatacgttta tcttcttcac tgatagcaaa gcggtcctgc ggacgcgtcg
    21841 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccag
    21901 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    21961 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    22021 tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca cgggtattgg
    22081 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    22141 cgaacccgcc cttgcgagcg ggataagaac ttaagcggtt tgcgccaggt gagcttcaat
    22201 tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc gcacgatggt
    22261 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa tcaccatatc
    22321 agcgctcgcc acatcttccg cagtcagttc attttccaga ccaattgacc cctgggtttc
    22381 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ctgccatata
    22441 ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca tggctcgttc
    22501 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    22561 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    22621 acagccactg ctgcgatata acccagcttg ccttcaacca ccggcagcac aatcagacca
    22681 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    22741 ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    22801 tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg ctcttctgcg
    22861 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg gatacaaata
    22921 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    22981 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag ccccatgatc
    23041 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    23101 gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt aatcagtgtg
    23161 ccgacgatag gaataatgaa gataggcatc accgagcgca gaactttatg caccggaatt
    23221 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagtgc accaaagaag
    23281 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    23341 tctgcaatgg aataaccgat gtacgctgcg aggaaaggca ccatcagcgt caagcccgca
    23401 acgccgatat caaacagttt tttcagattt ggatcggcta cggcatccgg cactgcgcct
    23461 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    23521 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    23581 ttcataagct gctcccgggc ttgcgaaagc ccatgtccat atttttgatg gaggaaacaa
    23641 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    23701 atcagtacaa aaatgcgatc cgcctcataa cttgcgataa accggaagca tgccgcaaaa
    23761 cagcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    23821 agatttgtca catggcaggc cgttgccgtg cgctcttatc ctgtaaacag aaaaccgtac
    23881 cgggagagag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    23941 tgcacgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatcac aaattgagtg
    24001 gcataacctg cgcactgacc gcaagggcaa cgccaaaagc gcccttgcgc tgattggcac
    24061 cgatacgctg gcgggcgata actgccagtt actgatttcc ggggccgacg aacaggaagc
    24121 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg cgccgctggc
    24181 ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    24241 aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga cgccgatctc
    24301 ttctttagat ctcaatgcgc tgggtaatct tcccgcagcc aaaagcgttg acgccgagca
    24361 atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc gtctgctgga
    24421 tagcgacggt gccaccagcg cgattctgga agcgcaccga tccctggctg gcgatacttc
    24481 cctgcgcgaa catttactgg caggcgtcag cgccggatta agctgcgccg aagcgattgt
    24541 caccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagct acctgcaaga
    24601 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    24661 acgcttcccg gcaccgggca aactgacgca gcccgccatt tgtatggctg atgaactgac
    24721 tcccagccag ttcctcgaac tggataaaaa tcacctcaaa ggtctgttac tgaaaagtgg
    24781 cggcaccacc tcacataccg taattcttgc ccgttcgttc aacattccga cactggttgg
    24841 tgtagatatt gatgccctta ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    24901 tggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc aagaagcccg
    24961 cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag cccgtaccgc
    25021 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    25081 attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct atatggatcg
    25141 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtctgc
    25201 caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac ccgttgatta
    25261 tctgaacatt ccggcagaag ctaacccgtt cctcggttat cgcgctgtgc gtatttatga
    25321 agaatacgcc tcgctgttca ccacacaact acggtcgatc ctgcgcgcct ccgctcacgg
    25381 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    25441 aaaactggcg gaagccaaac agcaactgcg taacgaacac attccgtttg atgagaagat
    25501 ccagcttggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc aatgctgcga
    25561 agagactgat ttctttagta ttggtagtaa tgacctgacg caatatctgc tggcagtgga
    25621 tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat tcttgcgggc
    25681 gctcgattac gccgtgcagg cggtgcatcg ccagggcaaa tggattggtc tgtgcggtga
    25741 gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg atgaactcag
    25801 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    25861 gtgccgcaag ttgctcaacc aagcaatggc ctgccgtact tcgctggaag tggaacacct
    25921 gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg agtgcatcac
    25981 gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    26041 gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg cgcgcgaggc
    26101 cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat cagaacacat
    26161 tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg gcgatgatga
    26221 agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc agcacatgcg
    26281 cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    26341 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    26401 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    26461 atattcccca ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    26521 atatgggaag ttctgccgcg tctgcaaaaa gcgattggtg atgagggcat tctgtttgct
    26581 cagactatga gtcgtgacgc gcagggtatg gtggaagaag cgaagcgcct gcgcgacgcc
    26641 attccgggca ttgtggtgaa aatcccggtg acttccgaag gtctggccgc cattaagatg
    26701 ctgaaaaaag agggtattac tacgcttggc accgcagtgt acagtgctgc acaagggtta
    26761 ctggccgcgc tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag
    26821 ggcggggacg gcattcgtac ggttcaggag ctgcaagcgc tgttagaaat gcacgcacca
    26881 gaaagcatgg tgctggcggc cagctttaaa acgccgcgtc aggcgctgga ctgtttactg
    26941 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    27001 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    27061 catctctaaa ggagcaatta tggaccacat tattcaatca ccgggtaaat acatccaggg
    27121 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    27181 ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa gctttaaaga
    27241 tgctggactg gtagtagaaa ttgcgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    27301 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta tcggtggcgg
    27361 aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    27421 accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct acaccgatga
    27481 gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca ttgtcgacac
    27541 caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg atgcgctggc
    27601 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    27661 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    27721 cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg agcgcgtgat
    27781 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcgggctgg cggcggctca
    27841 cgcggtgcat aacggcctga ccgctatccc ggacgcgcat cactattatc acggtgaaaa
    27901 agtggcattt ggtacgctga cgcagctggt tctggaaaac gcaccggttg aggaaatcga
    27961 aaccgtagct gcacttagcc atgcggtagg tttgccaata actctcgctc aactggatat
    28021 taaagaagat gtcccggcga aaatgcgaat tgtggcagaa gcggcatgtg cagaaggtga
    28081 aaccattcac aacatgcctg gcggcgcgac gccagatcag gtttacgccg ctctgctggt
    28141 agccgaccag tacggtcagc gtttcctgca agagtgggaa taacctactc caaactcccg
    28201 gcttgtccgg gagtttgaac gcaaaattgc ctgatgcgct acgtttatca agcctacgaa
    28261 atctctgcaa tatattgaat atgcgtgctt ttgtaggccg gataaggcgt tcactccgca
    28321 tccggcactc tgtgccagta atctgaaact cccacgttgt tcaggagttt tttatccgct
    28381 tctggacatt ttctccacag aacaatcgtt agcccggaag gtcgaatctg tgactcttat
    28441 tttcacggac gaaccagatg aaagcttcac tggcgctgct cagcctgcta acggcattta
    28501 ccagccactc cttaaaatct cccgccgttc caccaaccgt ggtacaaatc caggccaata
    28561 ccaaccttgc catcgcagac ggtgccagac agcaaatcgg cagcacgcta ttttacgatc
    28621 cggcgtatat gcagcttacc tatcctggcg gtgatgtccc gcaagaacgc ggggtatgtt
    28681 ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga tttgcagaaa ctggtgcacg
    28741 aagatatggc gaagaatttt gccgagtacc cgcaaaaatg gcagttaaag cgcccggaca
    28801 gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg gtttacccgc cacgataaaa
    28861 cacgccccat cagcaagaac cccagtgact atcaagcggg cgatattgtc tcctggcgac
    28921 tggataacgg actggcacat attggggtgg tgtcagatgg cttcgcccgc gacggtacgc
    28981 cgctggtgat acataacatt ggcgcaggcg cgcaggaaga agatgtgctg tttagctggc
    29041 ggatggtggg gcattatcgt tattttgtta aataagcgat cgccccacat gaagaaaaaa
    29101 ttcagatgct ttttacactg acagcttctt ttttcttacg gctcaccagc gccagtgcca
    29161 gcacaatcag cacaataccg ctaccttcaa cggcccccgg attctcaccg agcagccacc
    29221 aggaaaagag tacgccgcaa acgggaacgg ccagcgtgct taagctggca atactggcag
    29281 gcaagttttt caatacaaac aaccataagc tccacgccag cgccgtcgcc agaatcgcac
    29341 tgtaggccag cgcccagaac acggtgggct gccagtcaat ttcacgttgc ggcaccagta
    29401 aagcgaccac actcatcacc agcgccgcat agagcatctg ccaggatgtt aacgataata
    29461 aatccacgcg cggatgacgg gtgtataggc gtttagcaac aatcgcgctc gccccccaac
    29521 tgacgccgga gaggattgcc agcatggcac ttttcattga agagaaatcg agttgccacg
    29581 gctgcaacac caaaaataag ccgaaagcgg caatcagtat cgcgaaatat tgcccacgtc
    29641 gcaggcgttc accgagaaac aacgcggcga aaatcaccac ccagaacggc atggtatagc
    29701 ttaggatcgc caccttcccc gctccgccgc tgaccagcgc ccactgcgcc aggccaacca
    29761 tcccgcaggt ttgtaacagg gcaatggcta aggtgtattt aaacggtgtc gggcgcattc
    29821 cgcgaccacg taataaaagg acgatgaata aaacgagagc gccgaaaatg cagcgtaagg
    29881 cggtaaagtc gaaggcaccg atgtagctgg tgacttgctt catgaaaatc cagctataac
    29941 tccagataag tgtgagcaca accaggccac tgattgccag tgggttgctc tttcctgcgg
    30001 cagacatgag agatccagtg tgtagttttc ctgtggacac tatacgccgg taaagaaact
    30061 tcagcgactg cctgaaagac agtcgctgaa gaaggtcaga ttacagcagg tcgaaacggt
    30121 cgaggttcat cactttcacc catgccgcca cgaagtcttt aacaaacttc tcgtgggcat
    30181 cgctgctggc gtaaacttcc gctaccgcac gcaggacaga gttagaacca aataccagat
    30241 ccgcacggct ggctgtgtat ttcacttcgc cggtttcacg gtcacgacct tcgaacagct
    30301 ctttcgattc gtcagtcgct ttccactcgt aacgcatatc cagcaagttc acgaagaagt
    30361 cattgctcaa tacgccaacg cggtcagtga agacgccgtt tttgctgcca tcgaagttgg
    30421 cacccagtac acgcatcccg cccaccagcg cagtcatttc cggcgcggtc agcgtcagtt
    30481 gctgtgcttt gtcgatcagc agtgactcgg tggtggaaac gtccagacga gcgcgatagt
    30541 tacggaaacc gtcagcaatt ggctccagca gctcaaacat ctcaatgtca gtctgatcct
    30601 gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat gctcaaacct gcggcgcttg
    30661 cggctttctc aacaccaacc acaccagcca gcacgatgat atccgccagc gaggctttac
    30721 cagactcttt ctggattttc tccagaacag gcagagcacg aacggctgcg gcgttcacat
    30781 cccagtcgcg ctgcggcatt aatgccagac gcgcaccgtt ggcaccaccg cgtttgtcgc
    30841 caccacggaa ggtagaagca gaagcccagg ccaccgatac cagctcacta acagacagac
    30901 cagaatccgc aatcgcgaat ttcagatcga taatgtcctg ctcggtcggg ttgtagatcg
    30961 gctgcggcag cggatcttgc cagatcagat cttctttcgg cacttccggc ccgatgtagc
    31021 gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca ggcacgggca aaggcttcgt
    31081 tgaacgcctg cggatcgttg aggaaacgac gagagatctt ctcgaactca ggatcaaaac
    31141 gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt cttcgacgga tcaaacggat
    31201 ccgggataat ttccggtgcg tctaccgctt cgaactggat tgcgccagcc gggctgcggg
    31261 tctgtaccca ctcatacttg aacaggttct cgaagaaata gttgctccac tgggtcggcg
    31321 tctgggtcca gactacttcc agaccagagg taatggcatc tgcgccaacg ccgctgccgt
    31381 aagtgctcgc ccaacctaaa ccttgttctt caatcggtgc agcttctgga tcaggaccta
    31441 catttgatgt cggaccggca ccgtgggttt tacccagcgt atgaccaccc gcaataagcg
    31501 ccacggtttc ttcgtcgttc atgcccatgt tgccgaaggt cgcacggata gctgctgccg
    31561 cagaaagcgg ttcgccgctg tgatccgggc cttccgggtt aacgtaaatc aggcccatct
    31621 cggttgcacc cagcggtgct ttcgccagcg cttccggatg acggtgggtc agccaggctt
    31681 tttcatcacc ccagttaaca tccagatccg gttcccagac gtcttcacga ccggcaccaa
    31741 aaccgaaggt acggaagccg gagttttcca gcgccacgtt acccgcgagg ataaacaggt
    31801 cggcccagga gattttctga ccatatttct gtttgattgg ccacaacagg cgacgcgctt
    31861 tatcgaggct tacgttatcc ggccaggagt tcagcggtgc aaaacgttgc tgaccacgac
    31921 ccgcgccgcc gcgtccatcg attgaacggt aagtccccgc gccgtgccag gccatacgaa
    31981 taaacagacc ggcgtagctg ccccagtcgg caggccacca cggctgagat tctgtcagca
    32041 gggctttcag atcttttttc aggccgtagt aatctaattt gctgaattct ttgcggtagt
    32101 caaagtcctc acccagcggg ttagaacgat tagaatgttg gtttaacagg tcaacacgga
    32161 gttgatttgg ccaccagtcg cgagtggttg tgcccgcccc cgcgctctgg tcgtgaccgc
    32221 cctgatggaa cgggcatttg ccagtggctg tcgtgttatg gatatcgtct gacgtgctca
    32281 tcaatgtgct cccctctaca gtgttaccgt tacgatacac agcgttagag agaagttata
    32341 attgaattaa tccacggatg cgatagttga gatcttacat attgttggtt aaagagatgt
    32401 aggtcaaatt gatcgtaatt aagctttttt gattatgtcc gaattcggac aaatgcttta
    32461 taaaaaaggg ttttagcttc atatccttca agtaatgagg gagaaaacaa ggctgacaac
    32521 cttattttcc cgccctcatt tcgaggcagc attttgtgct ctgtttaaaa tttgtgatca
    32581 ctgtgtgatt ttcacaaaag ccacactatt tataaaccag gtcgaacacc cagcgtatgg
    32641 caaatcgcgt aactcatttc agcgcggtta agcgtataga agtggaaatc tttcactcct
    32701 tcacggctta aaatcttcac catatccatg gcaatattcg ctcccaccag tttgcgggtt
    32761 tcggcatcgt cgtccagccc gtcaaacatt tgtgccatcc acgccggaat acgcacgttg
    32821 gtcatatcgg caaatttctt cgcttgttta aagttcgaga ccggcaaaat tcccggaata
    32881 atttccacat caatacccgc cgatacgcag cggtcacgaa aacgcaggta gctttcgaca
    32941 tcaaagaaga actgagtaat cgcgcggttg gctccggcat cgactttgcg tttcaggttg
    33001 agcaaatccg cctgagcgct ttttgcttcc gggtgaacct ccggatacgc cgccacggag
    33061 atatcgaaat ctgccacttc ttttaacagc gtcaccaggt cagaagcata tatttccggc
    33121 ttaccacttc ccggcggcag atcgccacgc agcgccacga tatgacgaat accgttattc
    33181 cagtagtcgc gtgcaatggt gcgcagctcg tcgggcgtcg catcaatgca agtaagatgc
    33241 ggtgccgctt ccagaccagt gcgatcttta atgcctttaa taatgctgtg cgtacggtcg
    33301 cgctcgccgg agttcgcgcc ataggtcacc gatacaaact tcggtttcag gctgctaagg
    33361 cgatcgatgg agttccacag ggtctgctcc atttcactgg tacgcggcgg gaaaaactcg
    33421 aacgaaacgt taatttgccc ctggacttct gccaggctct gattcagggc atcccgctgg
    33481 ctggcgtgaa aaaagctcat accttacctc atcaatcgct tgtagttata tgttgtgttg
    33541 tgcacatcta tccgtttaga cgtccagatg taaagatgaa ggaaaagccg aagggcgtca
    33601 accgaaaaat gaccaatgat ggtgaggaaa aatcagggaa gatgaaaaaa cttcaggatg
    33661 gtaagaaaaa gaaaatccgg tatgcaaagc aaaccggact gcaaatataa actaaccatg
    33721 tacttattgc ttcttgccgg attcgtcgta aacgcttaat ccggcctaat cctatgataa
    33781 ttcaatatat ttcattagaa accataaacc cgaaaacatg agtaccgggc attattaaat
    33841 ttctgaaatt acaacaactg tgccagccgg ttgatatccg actgaatcgc cccggcggtg
    33901 acgtcgcgcc ccgcgccagg cccacggatc accagcgggt tgtcgcgata ccagcggctt
    33961 tcgatggcaa agacgttatc gcacggcagc agtgatgcca gcggatggtc ttcacgcacc
    34021 gcctccacgc ccacacgcgc tttgccgttg gcatcgaaac gcgccacgta gcgcagcacc
    34081 agccccattt cgcgggccgc ttccagccgt tgcaccatct gctcgttaag ttcatcgcca
    34141 ttttcaaaga aatggtcgat gctgccgcct tcgcaatgag caggcaccag cgattccaca
    34201 cgtacctggt ccggttcaat gttgtaaccc gcttcacgcg ccagaattac cagcttgcgc
    34261 attacgtctt tgccggagag gtcgtcacgc ggatcaggct cggttaagcc ctgctgccac
    34321 gcctgatcca ccagctcggt aaacggcacg ctaccgtcga attgcaggaa cagccaggag
    34381 agcgtgccgg agaagatccc gctgatcgac aaaatagtat cgccgctgtc gatcagatcg
    34441 cgcacggtgt ggttgatcgg caagcccgca ccgacggtgg cattgtacag ccagtgacgc
    34501 ccggtttttt cgaaggcgtc gtggatctgg cgatatttat tgctgtcgct ggctcccgcc
    34561 agtttgttgg cgctgataac gtggaaaccg tggctggcga aatcaagata ctgatcagca
    34621 agctgctggc tggcggtaac gtccagcacc actaaatcat catacggatg ggcgcgcatc
    34681 cacaggaaca acgactcttc atcctgctca accgcttcat cgttgaagaa ggctaacgcg
    34741 cggctggcgt ccagcccgtc atagctcaac aggctgcggc ggctgtccac cacacctgcc
    34801 agcacaaact caaagccggt acgtgccgaa agcgtgctct gctcacgggc gaacagttcc
    34861 agccaacggg aaccgatatt gcccttaccg aacaatacca ggccgatgcg tttttctgcg
    34921 cggaagacgg actgatgcag cccctgaatc aggctttcgg tcgggccggt gcgcagtact
    34981 gccaccaggc tgatgccgtc atcggactgc caggtaaatt cgaccggctg gcctttcagt
    35041 tgctgccaga agcggtggca atgcagcggg ttacgggtga cgcctgcacc gaccatcgcc
    35101 accagcgcca gcccctgacg caggcgcagt tcgccaggta atcccgcttc gtcgaggatt
    35161 ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt gcagcaactg gcgatcgtta
    35221 tgtacgccaa ccgccagcgg gcgtacctgc gcgcgtttca ggatttggtc gatctcttta
    35281 tgcgccagtt tgaaatcctg actggcgggc acctgaaact caatcaaaca gacatcatcg
    35341 tggctggtga caatacgcgc accagtaccg gaggccagca cgcgttcaat gcgcgtggaa
    35401 ccttgatccg gcgtgtagct acagcgcagt tgcaggtcga tttcgctgcc agaaaccggc
    35461 tgtaaagtac gggcgtgaag aacgggagcc gccaggcgcg ccagttcgct ggcctcatcc
    35521 agacgcagca acggcagcag gcaggcatct ttcactttac gcgggtcggc actgtatacc
    35581 ccggcgacgt cgctccagat ggttacgcga gaaacacccg ccagcgcacc gatttgtgtc
    35641 gcggaatagt cggaaccgtt acgccccagc agcaccgttt caccggcgtt gttgcggctg
    35701 ataaatccgg tcaccaccag acgtttgccc ggatgttgca ccagcagctg ttgcagcaac
    35761 gggtaagaaa gcccttcatc aacctgcggt tgtgcggcgc gttcagcgcg taaaaactcg
    35821 cgggcatcaa gccaggccgc tggcagccct tgttgattaa gtaccgcaga catcaggcgt
    35881 gccgaccaca cttcaccgtg ccccaccact tccgcataca ctgcgtcgtt aataccgctg
    35941 tcgagcagcg ccgccaggcg ctcaaggtcg ctgacaaaag cgctaatgag gctatcggct
    36001 tcttcagcgg gtagcagacc gctaatcaga tcgcactgat agcgacgcag cgtttgttga
    36061 acctgatgcg cagagagacg atcggtctgg cttagtttca accagttaat caactggtta
    36121 gtggtgctac cggcggcgga aaccaccatc atatcgtcag gctgagagta ctccgccata
    36181 atgcccgcga cacgcaaata acacttcaca tcagccagac tactgccacc aaatttatgc
    36241 agctgacgac ctttcgcccc tgcctgcgca atcacactca tttttacccc ttgtttgcag
    36301 cccggaagcc attttccagg tcggcaatta aatcttcgcc atcttcaata ccggtggaga
    36361 tacgcagcag cgtctcggag atcccggcgg cagcacgcgc ttctggtgcc atgcctgcat
    36421 gtgtcatggt tgcggcgtga gagattaaac tttccactcc ccctaatgat tccgccagcg
    36481 taaacaacga cagcccgctc aggaaacgac gcagcgtctg ctcatcgcca tccagttcaa
    36541 aactcaacat tgcgccaaag cctttttgct gacgcgcggc aatttcatgc ccctgatttt
    36601 ccggcagcga cgggtgatac agttttttca ccaacggctg ggtttgcaga tatttcacaa
    36661 tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac cagagttcgc aacccgcgta
    36721 gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc caccaggcca
    36781 gttcggtgac aacgtccgga tctttagcaa tcaccacgcc agccaccacg tctgagtgac
    36841 cgttcagata tttcgtgcat gaatgcaaca ccagatcggc acctaatgcc agcggatttt
    36901 gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact tcccttgcca
    36961 gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg ctttctacca
    37021 gtaccagttt gggtttttct gccagcgctg cccgtaatgc ctgttcatcg ccttgatcaa
    37081 caaacaacac gcgatagcaa ccgcgtttcg ccaggctgtc gaacaggcga tagctaccgc
    37141 cgtagcagtc gtgcggcgca accagcagat cgccaggttt caaaaagacg gtcgttacca
    37201 ggtgaatcgc ggacatgccg gtattagtaa gtactgcacc agcaccgcct tccagttctg
    37261 ccagcgcacg ctgaaccaca tcgcgcgttg ggttgccgcg acgcgagtaa tcatgcgcgc
    37321 gcggttcatt aaatccggta aagttatatg tgctggaaag atggatcggt gggacaacgc
    37381 aaccatactg ttcgtcgtca tttaacccgc tacgcactgc gatggtggcc tgtttacgcg
    37441 tcatgtgatg aagttccctg ggctttgtcg gtgaaatgtc aggcaccaga gtaaacattg
    37501 cgttaatgga cgtcaataca tctggacatc taaacttctt tgcgtataga ttgagcaaat
    37561 cccaaatagc cgttaaaatt atatgcatta tcacgccgac aggtgcatta cacgatgtca
    37621 cggtaacgcc tgtacggtaa actatgcggg tttacggtca gtacccacat aaattgtgtg
    37681 gtctggtctc aatttattga cgaagaggat taagtatctc atggctgaat ggagcggcga
    37741 atatatcagc ccatacgctg agcacggcaa gaagagtgaa caagtcaaaa agattacggt
    37801 ttccattcct cttaaggtgt taaaaatcct caccgatgaa cgcacgcgtc gtcaggtgaa
    37861 caacctgcgt cacgctacca acagcgagct gctgtgcgaa gcgtttctgc atgcctttac
    37921 cgggcaacct ttgccggatg atgccgatct gcgtaaagag cgcagcgacg aaatcccgga
    37981 agcggcaaaa gagatcatgc gtgagatggg gattaacccg gagacgtggg aatactaaag
    38041 cggaaaagag cggcgcggag ttaaccagcc gcttttttca tacaaggacg tttaacgatg
    38101 aaaatcaggc tactgatcct cagcctgctg gtttctgtac ctgcctttgc ctggcagccg
    38161 caaactggcg acatcatctt tcaaatctct cgctcatcgc aaagtaaagc gatccaactg
    38221 gcaacccatt ccgattacag ccacaccggt atgctggtga tacgcaacaa aaagccctac
    38281 gtttttgaag cggtcggccc ggtgaaatac accccgctca agcagtggat cgcccatggt
    38341 gaaaagggca aatacgtcgt tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa
    38401 aaactggcgc aaacggcaaa acgttatctc ggtaaaccgt atgatttcag cttctcatgg
    38461 agcgacgatc gccagtactg ttcggaagtg ggtggaaggt ttaccagaac gcgctgggaa
    38521 tgcgcgtggg cgaacagcag aagttgaaag agtttgatct cagcaacccg ctggtccagg
    38581 cgaagctcaa agaacgctac ggtaaaaata ttccgcttga ggaaacggta gtttcaccac
    38641 aagccgtttt cgacgcgcca caactcacca cggttgccaa agagtggccg ctgttttcgt
    38701 ggtaatggat gatgaaaaaa ataattcccg ccgtagtctg tttgttgctt tgtagttgca
    38761 cgacctttcc gcaagctgtc gctccggtaa acggtgatgc ctccaattag ttgaattaag
    38821 gatataatac tcgcttttaa ggttgtttca tggtcacagt taaccttcat tgcccccgtt
    38881 gccagtccgt tcaggtttac cgtcacgggc agaacccaaa aagacatgat cgattccgcg
    38941 gccgtgactg tcaccgggta tttcagctca gttactgtta cgagccccgt aaaccgggag
    39001 ttaaagacca aatcgctgaa atgactttca acggtgccgg ggttcgcgat actgcccgta
    39061 cgctgaaaat cggcattaat acagtcatcc gaactttaaa aaactctcgc cacgaagaat
    39121 aacgtcatcc ccggtcgctc atgctgatgt ggcactcatc tgcaaactta gtcagcaatg
    39181 gagctttgtc ggcagtaaag cgcgacagca ctggctctgg tacgtgtaca acacgaaaac
    39241 tggtggtgtt ctggcctaca cttttggccc ccggactgac gaaacctgtc gtgaattgcg
    39301 ggcgctgctt acccttttac catcggcatg ttgaccactg acgaatgggg tagttacgct
    39361 agagagttac cgaaggagaa gcatctgact ggtaagattt ttactcagca cattgagcgt
    39421 aacaatctga ccctgagaac ccgaatcaaa cgtctggccc gcagaaccat ctgtttctcg
    39481 cgttctgtag aacttcatga aaaagtcatc ggtgctttta tcgaaaatat atgttctact
    39541 aattggagtc atcacccttc tgataagctt tccttggatg attaccagac tgaatacaca
    39601 gccaaagatt tttacctcat tctgctcttt tttctcaaat ga
//
LOCUS       DAPPNJ010000016        39625 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000016 DAPPNJ010000000
VERSION     DAPPNJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.016"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            102..1514
                     /gene="cycA"
                     /locus_tag="RPM73_000629"
                     /gene_synonym="dagA"
     CDS             102..1514
                     /gene="cycA"
                     /locus_tag="RPM73_000629"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDX6519021.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(1559..2221)
                     /gene="ytfE"
                     /locus_tag="RPM73_000630"
     CDS             complement(1559..2221)
                     /gene="ytfE"
                     /locus_tag="RPM73_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDX6519022.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDGLMDHISLENNVLFPRALA
                     GE"
     gene            complement(2329..3294)
                     /locus_tag="RPM73_000631"
     CDS             complement(2329..3294)
                     /locus_tag="RPM73_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX6519023.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(3402..4262)
                     /gene="qorB"
                     /locus_tag="RPM73_000632"
     CDS             complement(3402..4262)
                     /gene="qorB"
                     /locus_tag="RPM73_000632"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDX6519024.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4351..4731
                     /locus_tag="RPM73_000633"
     CDS             4351..4731
                     /locus_tag="RPM73_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDX6519025.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGILILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(4860..6803)
                     /gene="cpdB"
                     /locus_tag="RPM73_000634"
     CDS             complement(4860..6803)
                     /gene="cpdB"
                     /locus_tag="RPM73_000634"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDX6519026.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDAKTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGSRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            6993..7733
                     /gene="cysQ"
                     /locus_tag="RPM73_000635"
     CDS             6993..7733
                     /gene="cysQ"
                     /locus_tag="RPM73_000635"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDX6519027.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(7723..8280)
                     /locus_tag="RPM73_000636"
     CDS             complement(7723..8280)
                     /locus_tag="RPM73_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDX6519028.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            8605..8811
                     /locus_tag="RPM73_000637"
     CDS             8605..8811
                     /locus_tag="RPM73_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDX6519029.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(8873..10216)
                     /locus_tag="RPM73_000638"
     CDS             complement(8873..10216)
                     /locus_tag="RPM73_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDX6519030.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(10539..11177)
                     /gene="msrA"
                     /locus_tag="RPM73_000639"
     CDS             complement(10539..11177)
                     /gene="msrA"
                     /locus_tag="RPM73_000639"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDX6519031.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            11383..13116
                     /gene="tamA"
                     /locus_tag="RPM73_000640"
     CDS             11383..13116
                     /gene="tamA"
                     /locus_tag="RPM73_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDX6519032.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPIGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            13113..16892
                     /gene="tamB"
                     /locus_tag="RPM73_000641"
     CDS             13113..16892
                     /gene="tamB"
                     /locus_tag="RPM73_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDX6519033.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            16895..17236
                     /locus_tag="RPM73_000642"
     CDS             16895..17236
                     /locus_tag="RPM73_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDX6519034.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            17448..17699
                     /gene="chpS"
                     /locus_tag="RPM73_000643"
     CDS             17448..17699
                     /gene="chpS"
                     /locus_tag="RPM73_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDX6519035.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            17693..18043
                     /gene="chpB"
                     /locus_tag="RPM73_000644"
     CDS             17693..18043
                     /gene="chpB"
                     /locus_tag="RPM73_000644"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDX6519036.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPIRQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(18123..18653)
                     /gene="ppa"
                     /locus_tag="RPM73_000645"
     CDS             complement(18123..18653)
                     /gene="ppa"
                     /locus_tag="RPM73_000645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDX6519037.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            18963..19919
                     /gene="ytfQ"
                     /locus_tag="RPM73_000646"
     CDS             18963..19919
                     /gene="ytfQ"
                     /locus_tag="RPM73_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDX6519038.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            20229..21731
                     /locus_tag="RPM73_000647"
     CDS             20229..21731
                     /locus_tag="RPM73_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX6519039.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFVLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            21745..22767
                     /locus_tag="RPM73_000648"
     CDS             21745..22767
                     /locus_tag="RPM73_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6519040.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            22754..23749
                     /gene="yjfF"
                     /locus_tag="RPM73_000649"
     CDS             22754..23749
                     /gene="yjfF"
                     /locus_tag="RPM73_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDX6519041.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23782..24780)
                     /gene="fbp"
                     /locus_tag="RPM73_000650"
     CDS             complement(23782..24780)
                     /gene="fbp"
                     /locus_tag="RPM73_000650"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDX6519042.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            24956..26329
                     /gene="mpl"
                     /locus_tag="RPM73_000651"
     CDS             24956..26329
                     /gene="mpl"
                     /locus_tag="RPM73_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDX6519043.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(26480..27031)
                     /locus_tag="RPM73_000652"
     CDS             complement(26480..27031)
                     /locus_tag="RPM73_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDX6519044.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            27125..28477
                     /gene="pmbA"
                     /locus_tag="RPM73_000653"
     CDS             27125..28477
                     /gene="pmbA"
                     /locus_tag="RPM73_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDX6519045.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSSVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            28660..29046
                     /gene="cybC"
                     /locus_tag="RPM73_000654"
     CDS             28660..29046
                     /gene="cybC"
                     /locus_tag="RPM73_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDX6519046.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(29091..29555)
                     /gene="nrdG"
                     /locus_tag="RPM73_000655"
     CDS             complement(29091..29555)
                     /gene="nrdG"
                     /locus_tag="RPM73_000655"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDX6519047.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(29713..31851)
                     /gene="nrdD"
                     /locus_tag="RPM73_000656"
     CDS             complement(29713..31851)
                     /gene="nrdD"
                     /locus_tag="RPM73_000656"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDX6519048.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVKAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(32245..33900)
                     /gene="treC"
                     /locus_tag="RPM73_000657"
     CDS             complement(32245..33900)
                     /gene="treC"
                     /locus_tag="RPM73_000657"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDX6519049.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(33950..35371)
                     /gene="treB"
                     /locus_tag="RPM73_000658"
     CDS             complement(33950..35371)
                     /gene="treB"
                     /locus_tag="RPM73_000658"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDX6519050.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(35490..36437)
                     /gene="treR"
                     /locus_tag="RPM73_000659"
     CDS             complement(35490..36437)
                     /gene="treR"
                     /locus_tag="RPM73_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDX6519051.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            36622..36675
                     /gene="mgtL"
                     /locus_tag="RPM73_000660"
     CDS             36622..36675
                     /gene="mgtL"
                     /locus_tag="RPM73_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDX6519052.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            36816..39512
                     /gene="mgtA"
                     /locus_tag="RPM73_000661"
     CDS             36816..39512
                     /gene="mgtA"
                     /locus_tag="RPM73_000661"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDX6519053.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
BASE COUNT         9316 a        10025 c        10746 g         9538 t
ORIGIN      
        1 ttacctaata tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg
       61 tagagcctga acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag
      121 tcgttgccga tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac
      181 atattcagct tattgccatt ggcggtgcca ttggtacggg gttgtttatg gggtccggca
      241 aaacgattag ccttgccggg ccgtcgatca ttttcgttta tatgatcatt ggttttatgc
      301 tctttttcgt gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt
      361 ttagtgactt cgcttccgat ttactcgggc cgtgggcagg atatttcacc ggctggacct
      421 actggttctg ctgggttgta accggtatgg cagacgtggt ggcgatcacg gcttatgctc
      481 agttctggtt ccccgatctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc
      541 tgacgctcaa ccttgccacc gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga
      601 tcaaaatcgt cgccatcgtg tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact
      661 ttcagtcacc gactggtgtg gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt
      721 tcccgaaagg tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtgg
      781 ggattgagct ggtaggtaca acagctgcgg aaaccaaaga tccggagaaa tcactgccac
      841 gcgcgattaa ctccattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtga
      901 ttatgtccgt gacgccgtgg agttcggtag tcccggagaa gagcccgttt gttgaactgt
      961 ttgtgttggt aggtttgcct gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg
     1021 cggcgtcttc cgctaacagc ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc
     1081 aggaaggtgt ggcaccgaaa gcgttcgcta aactttctaa gcgcgcagta cccgcgaaag
     1141 ggttgacctt ctcgtgtatc tgtctgctcg gcggcgtggt gatgctgtat gtgaatccca
     1201 gcgtgattgg cgcgttcacg atgatcacga ccgtttccgc gattctgttt atgttcgtct
     1261 ggacgattat cctttgctcg tacctggtgt accgcaaaca gcgtcctcat ctgcatgaga
     1321 agtcgatcta taagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg
     1381 tgttcgtggt cgtgttgctg acactggaag atgacactcg ccaggcgctg ttggtcaccc
     1441 cgctgtggtt tatcgcactg gggctgggct ggttgtttat tggtaagaag cgggctgctg
     1501 aactgcggaa ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatt
     1561 actcacccgc cagcgcgcgt gggaacagta cattgttttc caggctgatg tgatccatca
     1621 ggccatcaat cagttcatta atgccgttat acatcgcttt ccaggtggtg caggcttctg
     1681 gcggcggtgt gacgttattg gtagtgtgtt tgatcacttc cagcagttcg cccgcttcat
     1741 cgtgctcgct ttccattacg ctgattggcc ccattgcctg gctgcccatg ccttgtttga
     1801 tcatcgggaa gaggatctgc tcttctttca tcatgtggct ggaaagctct tcatgcagca
     1861 tagtcaggta ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgctcga
     1921 ctttagtcgc ttgcaggatc agctccggca gctgctcgcg gtgacgatcg tggtagcgca
     1981 cgatgatatg gtcaatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt
     2041 gttcagcaag ctttgccagt tcagcttcaa tgacctcaac atccagttct ttacgtgccg
     2101 ccgcgcgcgc cagcgtctgc ttaccgccac agcagtaatc catatcatat ttacgaaaca
     2161 gagccgaagc gcgagggata gagagcgcca gttcacctaa aggttggtcg cgataagcca
     2221 tagctgatac ctcattctta ataagataag atgtatttta aatgcatctt taaggcaaaa
     2281 gctataaccc ttacgtagca gaggcttatt taactcactg ccagcgtgtc aactttctga
     2341 gacagattca gttaaaggtt tttctggttt tgctctgacc gcaatgacca cgccaatcac
     2401 taacagcgcg ataccgctca gcgtcattag cggcggcatt tgctggcgga gtaaaaaggt
     2461 gtacagcaaa cctgccagcg tttcgaaaac aatcagcggc ccgagaatca ctgtcggtaa
     2521 tcgctggctg gcgacgttcc agcagagtgc gccaacccat gagcaaagca cggctatcgc
     2581 aaccatcaga ctaataaaca ccagcggacg ggggccaaaa ggtaaggaga agtccggcgt
     2641 ttgtatattc agccagtaac aggcgacgag atagccgatg agagaaaccg gcagcgtgac
     2701 cagcgcctgc gccgtcgccc acatcatcgg atgtttgtcg ggattttccc gcagccagcg
     2761 ggcgttgcgc agggcatacc atgcccagca gaccacggaa actaacgcta gcacgatgcc
     2821 agaggtataa cgtgcccagt caaaatcggg gagtccgtgg tttaactcag caatattcac
     2881 actcgccagg ccgatgccaa tacaaatcag tgccggggcg agttttcccc acgcgagttt
     2941 gccgtcgcgc tggctataaa gcagattggc aaagacagga atgaccaccg gcagggtgcc
     3001 gataatcatc gtggaaacag gcgcgccagt acgttgaatg gcactggcaa ggcagaagta
     3061 ataaatgagg ttgcccatca tagtgagcat caaggcggta agccagtccc gacgcgccaa
     3121 ctgacgcaga cgcacgcgtc ccagccaggc aatgggcagc gcaattaacc ctaacgccag
     3181 ataacgcccc atcgactgca acatcgccgg gtattccggt acgatcaacg gcccgacaaa
     3241 aataagcccc cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg
     3301 catccattgg tctgtcagaa gaaagttcag gaagcagaca tgattgctcc cggcatcccg
     3361 tcattatcag agaggatgcg ggaggatgcc actttaatta actagttatt aacattaaaa
     3421 agatggctta cgctttcggc taacgttgtc gttgggcggc caatcaattt gctaagcgtt
     3481 ttgctgttat caaacagacc acctttcgat gcgccaacgt cagaatccgc cagcatatcc
     3541 gccagtccgt cgggcagtcc gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga
     3601 ttttgatagg taacggattt tccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac
     3661 gtccaggcac tatcgcccgc cagttcgtaa accttgcctt cgtgaccggc ttcgctaatc
     3721 acgcgtgccg cagctgccgc ataatctgcc cgcgttgctg aggcgatttt gccatcgccc
     3781 gccgcaccga taaatacgcc gtgttccagt gctgccgggg cgctggcgag gtagttttcg
     3841 gtgtaccagc cgttgcgcag cagggtgtaa acgatgccag aatcagccag cattttctcc
     3901 gtctcgatgt gctcatcggc gaggccgagc ggggaggtat ctgcatgtag caggctggta
     3961 taagcgataa atttcacgcc agccgccttt gcggcattaa taacattacg atgctgcggg
     4021 gcacgttgac ccacttcgct ggaagagatc agcagtagtt tttccactcc ctgaagtgca
     4081 gatgtcagtg cggcttcatc gccgtagtca gcctgacgca cggtaatgcc ttgtgctgcc
     4141 agggcctggg cttttgccgg attacgaacg atagccacta tttggctggc aggaaccgtt
     4201 ttcatcaagg attcaataac atagtgacca agttggccag tggcaccagt aatagcgatc
     4261 atgggaagtc tccatcgttt tgtcttgtgt ttaaatacgc taacacctaa acttactttt
     4321 agtaagtacg tacaaaaagg taagtatgaa atgagtcagg ttagcctgtc gcaacaactg
     4381 aaagagggta atctctttgc ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc
     4441 agccgttggg ggatattgat tctggtggcg ctacgcgaag gtactcatcg ctttagcgac
     4501 ctgcggcgca aaatcggcgg ggtgagcgaa aagatgcttg cgcagtcgtt acaggcgtta
     4561 gaacaggatg gttttcttaa ccgtatcgcg tatccggtgg tgccgccgca tgtggaatat
     4621 agcctcacgc cgctgggcga gcaggtgagc gaaaaggttg ctgcactggc ggactggatt
     4681 gagttgaatt tgcccgaggt gttggcggtg cgggatgaac gtgcggcata actgccggat
     4741 gcgctgagct tatctggtct acggtgcatg gcttgtaggg cggataagat gcgccagcat
     4801 cgcatccgcc aataaatgcc ggatgcggtt gatgatttat aggccgaaaa agaagtgttt
     4861 tacttactca aatccacctg ataaatcgca aacccgatat catcggtcgc gactttattc
     4921 atcggatatt gccctttctc tttaataaac gctgcggctt tatctgacgg ggaagtttcg
     4981 aaacggatat ccagtttctt atcgccagca atcggtgcta aacgccagtt gttatctgcc
     5041 gccgggtgaa tttcccccgc acgcttcgac tcatcagcaa tccacgctgc cagcaccgag
     5101 cggttctcat ccggtgaagc aaaagcgata tggctgtcgc ccgtaccggc aaatttgcca
     5161 ccgtaagcgc gatagttatt ggtggcaacc aggaacatag cgttcggatc aatcggcttg
     5221 ccattaaagg tcaggttctt aatccgctct gcattggcat taatcatctg gcactcgccg
     5281 tcataacggg cgggttgggt aacatcaatc tgataattca caccatcaat aacatcaaag
     5341 ttataagtgc ggaaaccatc ccagttgatg agtgactgtg gtttcgtgct gttaggatca
     5401 atctggttaa actgtccggc ggagcactcc agccactctt tcacctcttt accgctggct
     5461 ttcaccacaa tcagtgtatt ggggtagaga taaagatcgg cggcattacg gaaggtcaac
     5521 tggccttttt ccacctccac atagcttgcc gggtcatttt tgcgactacc aactttaaac
     5581 ggtgcggcag ctgaaagcac cggcagtttt gccagatccg gatcgccctg aatgtaatgt
     5641 tcgacatacg ctttttgcgc gttgttcacc acctgcacgg tcggatcgtc ctgcaccagt
     5701 gccagatagc tatacatatt gtcggcagat ttaccgattg gcttgctgac gaactggcgt
     5761 gtggcatcgt gatcggcttt gagtgtttct accagcttgc tgtcttccgc cgcgagggat
     5821 tttttattag cgatgtcgta aatcggtcgt gcttccgctt tcgcctgcgt cacctgccat
     5881 ttaccgctgt cattactgag ttgtaagtcg accacaccaa gatgatcgcc ccacatgcct
     5941 ggcattaccg ccggaacacc attcagcgtg cctttcgcga tatcagctcc ttcgatatca
     6001 gcaaaatctt tacttgggaa aacggcgtga gcatggccaa acataatggc gtcaacaccc
     6061 ggaatttcac tgagataata aactgagttt tccgccatca ctttatacgg atcggcagac
     6121 agcccggaat gcgccagaac gacaacgaga tcggcacctt tctcacgcat ttcaggcacg
     6181 tatttgcgca cagtttctgt tatatcgtta acggtgactt tgccggacaa attggcttta
     6241 tcccacccca taatctgcgg cggcacgacg ccaatatagc caatctttaa cgtctgtttt
     6301 tttccgtctt tatcgaccac ttcggtgtct ttaattaaat acggtgtaaa catgggctgt
     6361 ttggtttttg cgtcaatgac gttggcattt acataaggga atttcgctcc tgccaacgca
     6421 tttttcaggt aatccagacc gtagttaaac tcgtggttgc caagcgtgcc gacggtatag
     6481 tccagcgtat ttaatgcctt atagaccggg tgaatatctc cggcttttaa tcctttcgcc
     6541 gacatgtaat cggccagcgg acttccctgg atcaagtcgc cgttatccac cagcacgcta
     6601 tttttgactt cattgcgggc atcgttaatc aggcttgcgg tgcgtaccag tccgaacttt
     6661 tccgtggcgg tgtctttgta ataatcgaaa tccatcatgt tgctatgcag atcagtggtt
     6721 tccatgatac gcagatcgac cgtcgctgca ttcacactgg cggcaatcag cgtggccagg
     6781 agcgttgcgc taaacttaat catcagggac atccttttat tatcgggaat acgaaagaaa
     6841 agggagaata aacgtcttac ttatagaaca gtgaagaatg ccacaatttt acgctttgaa
     6901 aatgatgaca ctatcacagt tggcgcattc attaacgata gggtataagt aaaacaataa
     6961 gttaacaccg ctcacagaga cgaggtggag aaatgttaga tcaagtatgc cagcttgcac
     7021 ggaatgcagg cgatgccatt atgcaggtct acgacgggac gaaaccgatg gacgtcgtca
     7081 gcaaagcgga caattctccg gtaacggcag cggatattgc cgcacacacc gttatcatgg
     7141 acggtttacg tacgctgaca ccggatattc cggtcctttc tgaagaagat cctcccggtt
     7201 gggaagtccg tcagcactgg cagcgttact ggctggtaga cccgctggat ggtactaaag
     7261 agtttattaa acgcaatggc gaattcaccg ttaacattgc gctcattgac catggcaaac
     7321 cgattttagg tgtggtgtat gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca
     7381 aggcctggaa agaagagtgc ggtgtgcgca agcagattca ggtccgcgat gcgcgcccgc
     7441 cgctggtggt tatcagccgt tcccatgcag atgcggagct gaaagagtat ctgcaacagc
     7501 ttggcgaaca tcagaccacg tccatcggct cttcgctgaa attctgcctg gtggcggaag
     7561 gacaggcgca gctgtacccg cgcttcggac caacgaatat ttgggacacc gccgctggac
     7621 atgctgtggc cgcagctgcc ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt
     7681 acactccgcg cgagtcgttc ctgaatcctg ggttcagagt gtctatttat taattaattt
     7741 atgcagcagg tccatcactt gctgcacctc ttcctgagta agcgccccgt ctttggccca
     7801 ttgcacgcga ccgtctttat ccagcaccac tacagcggaa cttttttcat ccagctgcca
     7861 ggcaccgcgt gcgacgccat tgctatcaac aataaactgc gaccagggat aaagcttttt
     7921 attactctcc agactgctgc gcacaaacat gccggaaccc ggaattgcgt cgtcggtgtt
     7981 aacaatggtg gtggtctggt aacgatcgtg cggtaacttc gctgatttaa tcgcttcaat
     8041 cagcgtcgcg tttttctctt ttgcagaggt gcgaccagca atatgttgca gtactcgcac
     8101 ttttcccact aactgcgcgc tgttccaggt tttgtagcta aactgatctt tatcaagcac
     8161 caactcgcca cgatcggtga tgccaatcgg cggcactcgc tgaccggttt cgaactgatg
     8221 tgcggaagcc atcatcggca acagcaggca ggtgagtgcc agaatcttgc gtagggtcat
     8281 ggtgtttcct tcttatgata tgcaggtgat ccgaccactt gggtctgagt tttaatcata
     8341 tgtgctattt atcgaatttc ccgcaagtgt gatgccagtt tgcggtcaag cgcacaaatc
     8401 atatgaaaaa tgaatgctta tactgaagac cgcgcttcgg taaaaagata attctgaata
     8461 attgtaacct ttaggtaaaa aaagttatac gcggtggaaa cattgcccgg atagtctata
     8521 gtcactaagc attaaaattt gcgcctcata atagttgggc cgattgtggc accgcacagg
     8581 cgtaatactc agcaggagat aacaatgaaa attttccaac ggtacaaccc acttcaggtg
     8641 gcgaagtacg taaagatcct gttccgtgga cggttataca tcaaggacgt tggcgctttt
     8701 gaatttgata agggtaagat tcttatccca aaagtgaagg ataagctgca tttgtcagtg
     8761 atgtccgaag ttaaccgtca ggttatgcgt ctgcaaacag agatggctta accaaagtgc
     8821 tatgcagtaa taagacggct cctgattcag gagccgttga tgtttctggg ggttacgcga
     8881 cgctttcttc tttatcttta gcgtcaggca gttttggcga aagggcggtg gccttgctgt
     8941 cgatccgggt caccagcagc tggtcgatgc ggtagttatc gatatccacc acttcaaatt
     9001 tgtagccggc gaatttcacc gaatcggtgc gtttcgggat cttacgcagc ataaacatca
     9061 taaagccgcc gatggtttcg tagttgcccg actgcgggaa ttcgtcaata tccagcacgc
     9121 gcatgacgtc gtcaattggg gtgccgccgt caatcagcca tgagttctca tcacgggcga
     9181 caatctgctc ttccagcccc tgaccgacca gatcgcccat cagcgtggtc atcacgtcgt
     9241 tgagggtgat gatccccacc accagcgcgt actcgttcat gatcaccgcg aagtcttcac
     9301 ctgcggtttt aaaactttcc aacgcctctg aaagggttaa cgtatccggc acaatcagcg
     9361 tgttgcgaat ttgtacgccg ctgttcagtg ccaggctttg gttagccagc acgcggttca
     9421 gcaggtcttt agaatcgaca tatccgatga tgtggtcaat atcttcatta cagacgagaa
     9481 acttagagtg cggatgttcc gccaccttat tcttcaggct ttgctcatct tcgtggagat
     9541 caaaccaaat cacgttttca cgcggtgtca ttgaagacgg aacggtacgg gattccagct
     9601 caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa cacgcccgcc agcgcaccgg
     9661 cttccactac cgcgtagatg tcatcagaag tgatgtcatc tttacgtacc attggcagtt
     9721 tgaaaatacg gaagatcatg ttcgccaggc cgttgaagaa ccacaccagc ggggtgcata
     9781 cgtacaggca gaagcgcatc gggttgatga tacgcaaagc taccgcttct ggcgcaatca
     9841 taccgatgcg tttcggggtt aaatccgcaa acaggataaa catgccagtc actaacgaga
     9901 aagagagaat aaagctcagt tgctcagaga gttctgccga catatagcgg gagaacaggc
     9961 tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc gccgagaatc gccactgcgt
    10021 tcagaccgat ttggaccacg gtaaagaaca tgccgggatt ttcctgcata ttcagaacgc
    10081 gttgggcatt tatattgcct tcatcagcca gcagtttaag tttgattttg cgtgaggcgg
    10141 caagtgagat ctcagacatc gagaagaacg cacttacagc gatcaagcag agtatgacta
    10201 aaatactgtt taacatatct tatccgacct ttcaggccag atcctcggaa ggggaagttg
    10261 attaatttgt gtgaaataca cattgaaagc cgattcgaag aaagtgaacc ggcattttca
    10321 gtggctagta tagcgtaagg tactgtaaag tcgccagagg gttaaaattc agactgctga
    10381 taaatgcaat actgcctgat gcgctatgcc gatcaggcct acgcagtccc tgcaatatat
    10441 tgaatttgga cgattttgta ggcctggtaa ggcattcacg ccgcatctgg cataaacaaa
    10501 acgcacttta tcaacaatct acatttgtac ccgtaacgct atgcttccgg tggcagacag
    10561 acgccaattc cgccaattcc acagtaacca tacgggtttt tatgcagata ttgctggtgg
    10621 tcatcttcgg cataataaaa cggtgtggcg ttagcgattt ccgtggtgat gtgacgatcg
    10681 tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac tggcgcgagc tgcggcatcc
    10741 tgttctgggg tcagcggata aatcgctgaa cgatactgcg tgccgtggtc attgccctga
    10801 cgcattccct gggcgggatc gtgattctcc caaaatacct gtagcaactg ctcatagctg
    10861 atgacggaag gatcgtaaac aatgcgtacc gcttcggcat gacccgtatc accggagcac
    10921 acttcccgat aagtcggatt tggcgtatag cctccggtat agcctgcggc ggtgctgtaa
    10981 acgccgggta actgccagaa cagacgctcc acaccccaga aacaacccat cgcaaaaatg
    11041 gcaatctcca ttccgtcagg tacattggtc attgagtgac cgttgaccgc atgcagcgtg
    11101 gctacgggca tcggggtgtt acgtccaggc agggcatcgg cgggggaaac cagatgcttt
    11161 ttatcaaata aactcatggt gtcgctctcc cgaaaatcgg tcattggggt taaggttgta
    11221 acaagaggcg tatttgcaca caataaccac cgtgaatagg tctaaagtaa aacataagaa
    11281 atatttgggg tttagtctgc tttttaatcc atattactgg atttttgtta agccgtttaa
    11341 cggcgttcca ggggcaggaa aaaaggatat tcaggagaaa atgtgcgcta tatccgacag
    11401 ttatgctgtg taagcttact ctgcttaagc ggatctgccg tcgccgcgaa cgtccgtcta
    11461 caggtcgagg ggttatcggg acagctggaa aagaacgttc gtgcgcagct ttctacgatt
    11521 gaaagtgatg aagtgacgcc agaccgtcgc tttcgcgcac gcgtcgatga tgccatccgt
    11581 gaaggtctga aagcactggg ttattaccag ccgaccattg aatttgatct ccgtccaccg
    11641 ccaaagaaag ggcggcaggt attgatcgcc aaagtcacgc caggtgtgcc ggtgttaatt
    11701 ggcggcaccg atgtggtatt gcgcggcggc gcgcggaccg ataaagacta tttgaaattg
    11761 ctcgatactc gcccggctat tggcacggtg ctgaaccagg gcgattatga aaatttcaaa
    11821 aagtccttaa ccagcattgc gttgcgtaaa ggttatttcg atagcgaatt taccaaagcg
    11881 cagctgggca ttgcgctcgg cctgcataaa gccttctggg atattgatta taacagtggc
    11941 gaacgttacc gctttgggca tgtgaccttt gaaggatcac aaatccgcga tgaatacctg
    12001 caaaatctgg tgccgtttaa agagggcgat gagtacgaat cgaaagatct ggcggaactg
    12061 aaccgccgac tttctgctac cggctggttt aactcggtgg tggtggctcc acaatttgat
    12121 aaagcgcgcg aaacgaaagt attacccttg acgggcgtgg tttcgccgcg aactgaaaac
    12181 accatcgaaa ccggggtcgg ttactctacg gacgtgggac cgcgcgtgaa agcgacgtgg
    12241 aaaaagccgt ggatgaactc ttatggtcac agtctgacca ccagtaccag tatttccgcg
    12301 ccggaacaga ccctcgactt cagctataaa atgccgctgc tgaagaatcc actggaacaa
    12361 tattatttgg tgcagggcgg ttttaagcgc actgacctga acgataccga atctgactcc
    12421 actacgctgg tggcttctcg ctactgggat ctctccagcg gctggcagcg tgccattaac
    12481 ctgcgctgga gcctcgacca ctttacccag ggtgaaatta ccaacaccac gatgctgttt
    12541 tatcctgggg tgatgattag ccgcacgcgt tctcgtggtg gcctgatgcc aacctggggc
    12601 gactcgcaac gctactctat cgactactcc aacacggcct ggggctcaga tgtcgatttc
    12661 tccgttttcc aggcgcagaa cgtctggatc cgcacactgt acgatcgcca tcgttttgtg
    12721 acacgcggca cgctgggctg gattgaaacc ggtgatttcg acaaagtacc gccggatctg
    12781 cgtttcttcg ccgggggcga ccgcagtatt cgtggctaca aatacaaatc tatcgccccg
    12841 aaatacgcca acggtgacct gaaaggggcc tcgaagctga taaccggatc gctggaatac
    12901 cagtacaacg tgaccggaaa atggtggggc gcggtgtttg tcgatagtgg cgaagcggta
    12961 agcgatattc gccgcagcga ctttaaaacc ggtaccgggg ttggcgtgcg ctgggaatcg
    13021 ccgatcgggc caatcaaact cgattttgcc gtaccggtcg cggataaaga cgaacacggg
    13081 ttacagtttt acatcggtct ggggccagaa ttatgagttt atggaaaaaa atcagcctcg
    13141 gcgtggttat cgttatctta ctgttgctgg gatcggtggc gtttctggtg ggcaccacca
    13201 gcggcctgca tcttgtattt aaagcggcgg atcgctgggt gccaggactg gatattggca
    13261 aggtcaccgg cggctggcgc gatctcacct tgtctgacgt tcgttatgag cagccaggcg
    13321 tggcggtaaa agcgggtaat ctgcatctgg cggtcgggct tgagtgcctg tggaacagta
    13381 gcgtttgtat taatgacctg gcgctgaaag acattcaggt caacatcgac agtaaaaaaa
    13441 tgcctccttc tgaacaggtt gaagaagagg aagatagcgg tccgctggat ctctccacgc
    13501 cgtatcccat caccctgaca cgggtggcgc tggataacgt caacatcaag attgatgaca
    13561 ccacggtatc ggtgatggac ttcacctccg gcctgaactg gcaggagaaa accctgaccc
    13621 tgaaaccgac gtcgctgaaa ggcctgctga ttgctctgcc gaaagtggcg gaagtggcgc
    13681 aggaagaggt cgtcgaaccg aagattgaaa atccgcagcc ggatgaaaag ccgctcggcg
    13741 aaacgctgaa agatctcttt tctcgcccgg tattgccgga aatgaccgac gtgcatttgc
    13801 cgcttaacct gaacattgaa gagtttaaag gcgagcagtt acgcgtgacg ggcgacacgg
    13861 acatcaccgt gcgcaccatg ctgctgaaag tgagcagcat tgacggcaat actaaactgg
    13921 acgccctgga tatcgattcc aaccagggga tcgtcaacgc cagcggcacg gcgcagctgt
    13981 cagacaactg gccggtggat atcaccctca acagcacact gaacgtggag ccgttgaaag
    14041 gtgaaaaagt gaagctaaaa gtgggcggcg cgctgcgcga acagctggag attggcgtta
    14101 atctttccgg tccggtggat atggatttac gcgcccagac gcgactggcg gaagccggat
    14161 tgccgctcaa cgtggaagtg aacagcaaac agatttactg gccgttcact ggtgagaagc
    14221 agtatcaggc ggatgatctg aaactgaaac ttaccggcaa aatgaccgat tacacgctct
    14281 ctatgcgtac ggcagtgaag ggacaggaga tcccgcccgc gactattacc ctcgacgcga
    14341 aaggtaatga acagcaggtc aatctcgaca aactcaccgt tgcggcgctg gaaggcaaaa
    14401 ctgaactcaa ggcgttgctc gactggcaac aggccattag ttggcgcggt gagctaacgc
    14461 ttaatggcat taacaccgcc aaagagttcc cggagtggcc gtcgaaactc aatggcttga
    14521 ttaaaacccg cggtagcctg tacggcggca cctggcagat ggaggtgcca gaactgaagc
    14581 tgaccggtaa cgtcaaacag aacaaagtga acgttgacgg cacgctgaaa ggcaacagtt
    14641 atatgcagtg gatgatccca gggcttcatc tggaactggg gccaaacagt gccgaagtga
    14701 aaggcgagct gggggtaaaa gatctcaatc ttgatgccac catcaacgcg ccggggctgg
    14761 ataacgcgct gccggggctt ggcggtacag cgaaagggct ggtgaaagta cgcggcacgg
    14821 tggaagcgcc acaactactg gcagatatca ccgcgcgcgg cctgcgctgg caggaacttt
    14881 ccgtggcgca ggttcgcgtg gaaggcgaca ttaaatccac cgatcagatc gccgggaaac
    14941 tcgacgtacg cgttgagcaa atttcgcagc cggatgtaaa tatcaacctc gtcaccctga
    15001 atgccaaagg cagcgaaaag cagcacgagc tacagttgcg gattcagggc gagcctgtct
    15061 ccgggcagct taatctggca ggaagttttg atcgcaaaga agaacgctgg aagggaactc
    15121 ttagcaatac ccgcttccag acgccggtcg gcccgtggtc gctgacccgc gatattgcgc
    15181 tggattaccg caataaggag caaaaaatca gcatcgggcc acactgttgg cttaacccga
    15241 atgcggagct gtgcgtgccg caaactatcg atgcgggggc cgaagggcgt gcggtggtga
    15301 atctcaaccg cttcgacctc gccatgctga aaccgtttat gccagaaacc actcaggcca
    15361 gcggtatctt cacgggtaaa gcggatgttg cctgggacac cacgaaagag gggctgccgc
    15421 agggcagtat caccctttcg gggcgtaacg tgcaggtaac gcaaaccgtc aacgatgcgg
    15481 cgctgcccgt ggcgtttcag acgctgaatc tgacggcgga attgcgtaac aaccgtgccg
    15541 aattgggctg gaccatccgc ctgaccaata acggccagtt tgatggacag gtgcaggtga
    15601 ccgatccgca aggccgccgt aatcttggtg gcaacgtcaa tatccgtaac ttcaaccttg
    15661 cgatgataaa ccccatcttt acccgtgggg aaaaagcagc ggggatggtg agtgccaact
    15721 tgcgtctggg tggtgatgtg caaagcccgc agttgtttgg tcagcttcag gttacgggtg
    15781 tggatatcga cggcaacttt atgccgtttg atatgcaacc gagccagctt gcggtcaact
    15841 ttaacggtat gcgctcgacg cttgccggta cagtacggac ccagcagggt gaaatctacc
    15901 tgaacggtga tgccgactgg agccaaattg aaaactggcg ggcgcgggta acggcgaaag
    15961 gcagtaaagt gcggatcacc gtgccgccga tggtacgaat ggatgtatcg ccagatgttg
    16021 tattcgaggc tacaccaaac ctgtttaccc tcgatggtcg cgtggatgtc ccttgggcgc
    16081 gcatcgtggt gcacgatctg ccggaaagcg cagtaggcgt ctccagcgat gtggtgatgc
    16141 ttaacgataa cctgcaaccg gaagagccga aaacggcgtc gattccgatt aacagtaacc
    16201 tgattgtcca cgttggcaat aatgtgcgca ttgacgcctt tggcctgaaa gcgcggctga
    16261 cgggcgatct taacgtcgtt caggacaaac aagggctggg cctgaacggg cagatcaaca
    16321 tccctgaagg gcgcttccat gcctatggtc aggatctgat tgtgcgtaag ggtgagttac
    16381 tgttctctgg tccgccggat caaccgtatc ttaatattga agctattcgt aacccggatg
    16441 ctacagaaga cgacgtaatc gccggagttc gcgtcactgg tctggcggac gaaccgaaag
    16501 cggagatctt ctctgacccg gcgatgtcgc aacaagctgc attgtcttat ttgctacgtg
    16561 gacaagggct ggagagcgat cagagcgaca gtgcggcaat gacctcgatg ctgattggtc
    16621 tgggggttgc gcaaagtggc cagattgtgg gtaaaatcgg cgagacgttt ggcgtaagca
    16681 atttagcgct cgacacccag ggagtaggcg actcctccca ggtagtggtc agcggctatg
    16741 tattgccagg tctgcaagtg aaatatggcg tgggtatatt tgactctata gcaacactca
    16801 cgttacgtta tcgcctgatg cctaagctat atctggaagc cgtgtctggt gtagaccagg
    16861 cactggattt gctctatcag ttcgagtttt agcaatgcga atatttgtct acggcagttt
    16921 acgccacaaa caaggcaaca gtcactggat gaccaatgcc cagttactgg gcgatttcag
    16981 tatcgataac taccagttgt atagcctggg ccactatcca ggcgcagttc cggggaacgg
    17041 aacagtacac ggtgaagttt atcgtattga caacgccacg ctggccgaac ttgatgcctt
    17101 gcgcaccagg ggcggtgaat acgcgcgcca gttgattcag acgccgtacg ggagtgcatg
    17161 gatgtacgtt tatcaacgac ccgtcgatgg attaaagcta attgaaagcg gcgactggtt
    17221 agacagggat aagtaaccat atgcatacgc caccttcggg tggcgttgtt ttttgcgaga
    17281 cgactcgcat tctgttttgt aattccctca ccctttgctt ttctctccga gccgctttcc
    17341 atatctgtta acgcataaaa aactctgctg gcgttcacaa atgcgcaggg gtaaaacgtt
    17401 tcctgtagca ccgtgagtta tactttgtat aacttaagga ggtgcagatg cgtattacca
    17461 taaaaagatg ggggaacagt gcaggtatgg tcattcccaa tatcgtaatg aaagaactta
    17521 acttacagcc ggggcagagc gtggaagcgc aggtgagcaa caaccaactg attctgacac
    17581 ccatctccag gcgctactcg cttgatgaac tgctggcaca gtgtgacatg aacgccgcgg
    17641 aacttagcga gcaggatgtc tggggtaaat ccacccctgc gggtgacgaa atatggtaaa
    17701 gaaaagtgaa tttgaacggg gagacattgt gctggttggc tttgatccag caagcggcca
    17761 tgaacagcaa ggtgctggtc gacctgcgct tgtgctctcc gttcaagcct ttaatcaact
    17821 gggaatgacg ctggtggccc ccattaggca gggcggaaat tttgcccgtt atgccggatt
    17881 tagcgttcct ttacattgcg aagaaggcga tgtgcacggc gtggtgctgg tgaatcaggt
    17941 gcggatgatg gatctacgcg cccggctggc aaagcgtatt ggtctggctg cggatgaggt
    18001 ggtggaagag gcgttattac gcttgcaggc ggtggtggaa taaggtgtgt ttatttatcg
    18061 ctggcataaa aaaaccctta ctaaccgaag ctcggcgttc agggttattg cgctagaaga
    18121 acttatttat tctttgcgcg ctcgaaggaa gcaacgattt cagctttagc ggcttctgcg
    18181 ttttcccaac cttcaacttt cacccacttg cctttttcga ggtctttgta gtgctcgaag
    18241 aagtgagcga tctgcgcttt cagcagctct ggcagatcgt taacgtcttt aatgtgatcg
    18301 tattctttgc tcagcttgct gtgcggaacc gcaaccagtt tcgcatcttc accggcttcg
    18361 tcggtcattt tcagaacgcc aaccggacgg caacggatca cagaacccgg ctgcagcggg
    18421 tacggagtcg ggaccagtac gtcaaccggg tcaccgtcca gagacagggt gtggttgatg
    18481 taaccgtagt tgcacggata gaacatcgcg gtggacatga agcggtcaac gaacagtgcg
    18541 ccgctctctt tgtcgatttc gtatttgatc ggatctgcgt tagccgggat ctcaataaca
    18601 acgtagatgt cttccggcag atctttaccc gcagggacgt tgagtaagct catgtctgtt
    18661 tcctttaaaa atatgtggca aacaagtgcc gagtattata gccaactcgc gccgaatgtc
    18721 tttgcttgtt ttcgtcttca ttttcctttt tcgccagttt taagacggta tacagagcag
    18781 gaaaatccat aaccgtagcc gcatttttca tagtgagatg aaagcgatta caaacttgtg
    18841 attaacgttt tatttacttt tttgaagtgt gatgtaacgc aatccgttac ataacgaatt
    18901 gtctatagtt ttttcgcgaa catcttttca ccaataataa ttaccccgac gaggacaacc
    18961 ctatgtggaa acgcttactt gtagtctctg cagtctcggc agccatgtcg tctatggcgt
    19021 tggccgctcc attaaccgta ggattttcgc aggtcggatc ggaatccggc tggcgcgctg
    19081 cagaaaccaa tgtagcgaaa agtgaggccg aaaaacgcgg aattacgttg aaaattgctg
    19141 atggtcagca aaagcaggaa aaccagatta aagcggtacg ttccttcgtt gcacaagggg
    19201 tggatgcgat ctttatcgct ccggtggtcg cgacaggttg ggaaccggta ttaaaagagg
    19261 cgaaagatgc cgaaatcccg gtcttcttgc tcgaccgttc catcgatgtg aaagacaaat
    19321 ctctctatat gaccaccgtt actgccgaca acatcctcga aggtaagttg atcggtgact
    19381 ggctggtaaa agaagtgaat ggcaaaccat gcaacgtggt ggagctgcag ggcactgttg
    19441 gggccagcgt cgccattgac cgtaagaaag gctttgccga agccattaag aatgcgccaa
    19501 atatcaaaat catccgctcg cagtcaggtg acttcacccg cagtaaaggc aaagaagtta
    19561 tggagagctt tatcaaagcg gaaaacaacg gcaaaaacat ctgcatggtt tacgcccata
    19621 acgatgacat ggtgattggt gcaattcagg caattaaaga agcgggcctg aaaccgggca
    19681 aagatatcct cacgggttcc attgacggcg taccggatat ctacaaagcg atgattgatg
    19741 gcgaagcgaa cgccagtgtt gaactgacgc cgaatatggc aggtcccgcc ttcgacgcgc
    19801 tggagaaata caaaaaagac ggcaccatgc ctgaaaagct aacgctgacc aaatccaccc
    19861 tttatctgcc tgataccgca aaagaagagt tagagaagaa gaaaaatatg gggtattgag
    19921 ggtatcaatg cctgatgccg attcgtaggc cggataaggc gctcgcgccg catccggcgg
    19981 tggtgcgctg aagcctgatg cgacgcttac cgcgtcttat catgcctacg agttcggtgc
    20041 tctgtgtagg ccggataagg cgttcacgcc gcatccggca gtggtgcaac accggactta
    20101 acaccgtgca caatctgtcc cctcgcccct tcggggagag ggttagggtg agggggacaa
    20161 acggcgcagc tcttaacatc aggcacgatc tattcccaga cttttagagc gtggctaagg
    20221 gggaaaccat gacgaccgac caacaccagg aaatcctccg caccgaagga ttaagtaaat
    20281 ttttccccgg cgtcaaagcg ttagacaacg ttgatttcag cctgcgccgt ggcgaaatca
    20341 tggcgctgct cggtgaaaac ggggcgggaa aatcgacgct aatcaaagca ttaaccggtg
    20401 tataccacgc cgatcgcggc accatctggc tggaaggcca ggctatctca ccgaaaaata
    20461 ccgcccatgc acaacaactc ggcatcggaa ccgtttatca ggaagtcaac ctgctaccca
    20521 atatgtcggt cgctgataat ctattcatag gtcgcgaacc caaacgtttc ggctttctac
    20581 gccgcaaaga gatggaaaag cgtgccaccg aactgatgac atcttacggt ttctccctcg
    20641 acgtgcgtga accgcttaac cgtttttcgg tggcgatgca gcaaatcgtc gccatttgcc
    20701 gggctatcga tctctccgcc aaagtgctga tcctcgatga acccaccgcc agcctcgaca
    20761 cccaggaagt ggagctatta tttgtcctga tgcgtcagtt gcgcgatcgc ggcgtcagcc
    20821 tgatctttgt cactcacttt ctcgatcagg tctatcaggt cagcgatcgg atcactgtct
    20881 tacgcaacgg cagtttcgta ggctgtcggg aaacgcgcga gctaccgcag atcgaactgg
    20941 taaaaatgat gctggggcgc gagctggata cccacgcgct acagcgtgcc gggcgaacat
    21001 tgttgagcga caaacccgtt gccgcgttca aaaattacgg caaaaaagga acgatagcac
    21061 cgtttgatct cgaagtgcgc cccggcgaga tcgtcggtct ggctggcttg ctgggatccg
    21121 gacgtaccga aaccgccgaa gtgatcttcg gtatcaaacc tgctgacagc ggcacggcgt
    21181 tgatcaaagg caaacagcaa aacctgcgat cgccacatca ggcttcggta ctgggtatcg
    21241 gcttctgccc ggaagacagg aaaaccgatg gcatcatcgc cgccgcctcg gtacgggaaa
    21301 atatcatcct cgctctccag gcccagcgcg gctggctacg acccatttcc cgcaaagagc
    21361 agcaagagat tgccgaacgc tttatccgcc agcttggcat tcgcacacct tcaactgaac
    21421 aaccgattga atttctctct ggcggcaatc agcaaaaagt gttgctttca cgttggctac
    21481 tgacccgacc gcaatttctg atcctcgatg agccaacccg cggcattgat gttggtgccc
    21541 acgccgagat catccgcctg attgaaacgc tatgcgccga tggtctggcg ctgctggtga
    21601 tctcctccga actggaagag ctggtgggct atgccgatcg ggtgatcatc atgcgcgatc
    21661 gcaaacaggt ggcggagatc ccgctggcag agctttccgt tccggcgatc atgaacgcca
    21721 ttgcggcgta aggagaatag tgtgatgcct caatctctcc cggacactac gccgccgaaa
    21781 aggcgctttc gctggccaac gggaatgccg cagctggcgg cgctattgct ggtgctgctg
    21841 gtcgatagcc tggtagcccc gcatttctgg caggtggtgc ttcaggatgg gcgtttgttc
    21901 ggtagcccta tcgacattct taaccgtgca gctcccgttg cgttactggc gattggtatg
    21961 acgctggtga tcgccaccgg tgggattgat ctctccgtag gggcggtgat ggctatcgcc
    22021 ggagccacaa cggctgcgat gacggtcgcg ggattcagcc tgccgattgt tttgttaagc
    22081 gccctgggca ccggcatcct ggcgggattg tggaacggca tactggtagc gatcctcaaa
    22141 attcagccgt ttgtcgccac cctgatcctg atggtcgccg ggcgcggcgt ggcgcaactg
    22201 atcaccgccg ggcagatcgt cacgtttaac tcgccggatc tctcatggtt tggcagcgga
    22261 tcgctgttgt tcctgccaac gccggtcatt atcgcggtgc tgacgcttct cctgttctgg
    22321 ctgttgaccc gcaaaacggc gctggggatg tttatcgaag ccgttggtat caacattcgg
    22381 gcggcaaaaa atgccggggt aaacacgcgg atcatcgtca tgctcaccta cgtgttgagc
    22441 gggctgtgtg cggcgattgc gggcattatc gtggcggcgg atattcgcgg tgccgatgcc
    22501 aataacgccg ggttatggct ggagctggac gccattcttg cggtggtgat tggcggcgga
    22561 tcgctgatgg gcgggcgctt taacctgctg ctctcagtcg tgggggcgct gattattcag
    22621 gggatgaaca ccggaatttt actttctggc tttccgccgg agatgaacca ggtcgtaaaa
    22681 gcggtggtgg tgctttgtgt gctgattgtt cagtcgcaac gctttatcag tctgattaaa
    22741 ggagtacgta gccatgataa aacgtaactt gccgctgatg atcaccatcg gcgtctttgt
    22801 gctgggctat ctctactgcc tgacccagtt tcccggtttt gcttccacaa gagtgatctg
    22861 caatatcctg accgataacg cctttcttgg gataatcgcc gttggcatga cctttgtgat
    22921 cctctctggc ggtatcgatc tctccgtcgg ttcggtgatc gcctttactg gcgtgtttct
    22981 ggcaaaagtg attggcgatt tcggcctctc gccgctgctg gcgtttccgc tagtgctggt
    23041 gatgggctgt gcctttggcg catttatggg gcttttgatc gacgccctga agatcccggc
    23101 atttatcatt accctggcag gaatgttctt tttgcgcggc gtcagctatc tcgtttcaga
    23161 agagtcgatc ccgataaacc atcccattta tgacacgctc tcaagccttg cgtggaaaat
    23221 ccctggcggc ggtcgcttaa gtgcgatggg actgctgatg ctggcggtgg tggttatcgg
    23281 catattcctc gcgcatcgta cccgttttgg taatcaggta tacgccattg gcggcaacgc
    23341 aacgtcggca aacctgatgg ggatttctac ccgcagcacc actattcgca tttatatgct
    23401 ctccaccgga ctggcaacgc tggcggggat tgtcttctcg atttataccc aggccggata
    23461 tgcgctggcg ggcgtaggtg tggaactgga tgctatcgcc tcagtggtaa ttggcggtac
    23521 gcttttgagc ggtggcgttg gaacggtatt agggacgctt tttggcgtgg cgattcaggg
    23581 gctaattcag acttatatca actttgacgg tacgctgagt tcctggtgga cgaaaatcgc
    23641 catcggcatt ttgctgttca tttttattgc cttacagcgt ggattaacgg tgctgtggga
    23701 gaatcgtcag agttcgccag tgacaagagt caatattgcg cagcgataaa aacgcctctc
    23761 cgtgtggaga ggcgcaggag attacgcgtc cgggaattca cggataaagc gttcgacatc
    23821 ttcaaccata tggtcgttgc cgacaaagaa cgaacggcgc tggtgcagag tttccgggat
    23881 gatatccaga atacgctctt tgccatcgct cgctttaccg cccgcttgtt ccgccaggaa
    23941 tgccatcggg ttgcactcat acagcaaacg cagtttgccg tccgggtggc tggcggtgct
    24001 tgggtagaga taaataccgc ctttcagcag gttacggtgg aaatccgcga ccagtgaacc
    24061 gatataacgt gaggtatacg ggcggttggt ggatttatct tcttcctggc agaatttaat
    24121 gtacttcttc accccgttcg gaaacttaat gtagtttcct tcgttgatgg agtaggtttt
    24181 gcctttctcc gggaagcgca tccgttcctg gcacaggcag aaaacgccca gcgaaggatc
    24241 gtaagtaaag gcgtgaacac cgcatccggt ggtgtaaacc agcatagtag aggagccgta
    24301 taccacgtaa cctgccgcaa cctgtttgtt accaggctgg aggaaatctt cttccgttac
    24361 cggcgtgcca acaggcgtaa cgcggcggta gatggagaaa atggtaccga cagagacgtt
    24421 aacatcgatg ttggacgagc catccagggg gtccatcagc accacgtatt ttgcgtgttc
    24481 acagccttca aagacgacaa tctcatcttc ttcttcagag gcaatgcccg caacgatatc
    24541 gcgtgctttc agtgcggctt tcagtttttc attagcgaac aagtcgagtt tctgctgaac
    24601 ctcgccctgc acgttctcag caccgctggc acccaagata tcaaccagtc ctgctttgtt
    24661 gatatcgcga tggataatct tggcgcccag ttttattgcc gacagcaaag cagtgagctc
    24721 accggtagca tgagaaaact cgtgctgctt ttcgacaata aattcaccta acgttttcat
    24781 aaaactttcc ctgcaatgtt tatggagtaa agcgaccgca acaatcttaa caaataatct
    24841 caatgttgcg ctcaggtgaa tcgcgccagc aaattgcgga ttatcctgaa atgcgtttct
    24901 cacttgcccg acatatgcgt aaaatgagcg gcagattaaa aaaggatagt gacgtatgcg
    24961 cattcatatt ttaggaattt gtggcacatt tatgggcggt ctggcgatgc tggcgcgcca
    25021 gttaggccat gaagtaacgg gttcggacgc caatgtgtat ccgccgatga gcaccttact
    25081 tgagaagcaa ggcattgagc tgattcaggg ttacgatgcc agccagctcg atccgcagcc
    25141 ggatctggtg attattggca acgccatgac ccgtggaaat ccgtgtgtgg aagcggtact
    25201 ggaaaaaaac atcccttata tgtcaggtcc acagtggctg cacgattttg tgctgcgcga
    25261 ccgctgggtg ctggccgttg ccggtacaca cggcaaaact accaccgcgg gaatggcgac
    25321 ctggattctg gaacagtgcg gttacaaacc gggatttgtg atcggcggtg tgccggggaa
    25381 ctttgaggtt tcggcgcgtc tgggcgaaag cgacttcttt gttatcgaag cggatgagta
    25441 tgactgcgcc ttcttcgaca aacgctctaa atttgtccat tactgcccgc gtacgctgat
    25501 cctcaacaac cttgagttcg atcacgccga tatctttgac gacctgaaag cgatccagaa
    25561 acagttccac catctggtgc gtatcgttcc ggggcagggc cgtattatct ggccggaaaa
    25621 tgacatcaac ctgaaacaga ccatggcgat gggctgctgg agcgagcagg agctggtggg
    25681 tgagcagggt cactggcagg cgaaaaagct gaccactgac gcttctgaat gggaagtctt
    25741 gctggatggc gaaaaagtgg gcgaagtgaa atggtcgctg gtgggcgaac ataatatgca
    25801 caatggcctg atggcgattg cggcggctcg ccatgttggt gtagcgcctg cagatgccgc
    25861 taacgcgctg ggttcgttta ttaacgctcg tcgccgtctg gagttgcgtg gtgaagcgaa
    25921 tggcgtgacg gtatatgacg attttgccca tcacccgacg gcaattctgg caacgcttgc
    25981 ggcgctgcgt ggcaaagttg gtggtacggc gcgcattatt gctgtgctgg agccgcgctc
    26041 gaataccatg aaaatgggga tctgcaaaga cgatctggca ccttcattag gtcgtgccga
    26101 tgaagtcttc ctgctgcaac cggcgcatat tccgtggcag gtggcagaag tggcagaagc
    26161 ctgcgttcag cctgcacact ggagtggcga tgtggatacg ctggcagata tggtggtgaa
    26221 aaccgctcag cctggcgacc atattctggt gatgagcaac ggcggttttg gtgggatcca
    26281 tcagaaactg ctggatggtc tggcgaagaa ggcggaagct gcgcagtaat tcggcttcag
    26341 cctgatatag cattgccgga taaggcgttt acgccgcatc cggcatttga gcatagtgcc
    26401 tgatgcgacg cttgatgcgt cttatccggc ctacaggtgc accgcatccg gcaaaccata
    26461 ctcacatcaa caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa
    26521 tctggcgtgc ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttacggatca
    26581 gagtacgcag ctgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt
    26641 catcaccttg atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct
    26701 gctggttgtg acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct
    26761 ggcgcagcat cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct
    26821 gggcgagctc aatagccgcg cgcagatccg catctaacgg gatcttatcc agcgcgtttt
    26881 tccccagatc aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt
    26941 cacttttact gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt
    27001 cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg
    27061 ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta
    27121 cattatggca cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga
    27181 agaagcagtt tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc
    27241 cgtcagcaag accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga
    27301 attcaatagc gatggcgcgc tggggatcac tgtttatcac cagaaccgca aaggtagcgc
    27361 atcatccacc gatttaagcc cgcaggccat tgcccgtact gtacaggcgg cgctggatat
    27421 tgcccgttat acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt
    27481 tgacgcacca gatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga
    27541 actggcggcc cgcgcagaac aggcagcatt acaggcggat aaacgtatca ccaataccga
    27601 aggtggcagc tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt
    27661 gcagggttac tgctcaacgc gtcattcgct ctccagcagt gtaattgccg aagaaaatgg
    27721 cgatatggag cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc
    27781 agagtgggtc ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact
    27841 ctccaccatg aaagcgccag tcatttttgc caatgaagtg gcaaccgggc tttttggcca
    27901 tctggtgggg gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc
    27961 gctgggtaaa caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa
    28021 agggctggcg tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat
    28081 taaagatggc atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct
    28141 gaaaagcacc ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag
    28201 cttcgaacaa atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca
    28261 gggcgtgagt gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa
    28321 tggcgaaatt cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg
    28381 gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc
    28441 tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc
    28501 tataaactaa aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg
    28561 gccggggagc gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca
    28621 gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct
    28681 attcttgcag tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac
    28741 aatatggaaa ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa
    28801 gtcaaagacg cgttaacgat gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg
    28861 ccgaagctcg aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc
    28921 gacattctgg tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa
    28981 gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat
    29041 cgttaattcc tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa
    29101 tgatgcacca cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc
    29161 acaaatttgc cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg cgcagcgttg
    29221 agttcgtcga gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg
    29281 atgcgtttta ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg
    29341 gagagggaaa tcccctggcg tttgatacga gtgtcattca gatctttaat gatcttgtct
    29401 tccattgctt tggtaaatgg ctgaccagaa tttacccgcc atgtgctttt gttatagcaa
    29461 ccggggcatt catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg
    29521 acgatgtcga caggatagta ctgatgataa ttcattattt cgcctggctt atcagtccgc
    29581 gtgtaggctg catccggtaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta
    29641 cagccgttgc caaatgtagg ccggataagg cgtttacgcc gcatccggca tttgcttagc
    29701 gcagagtgaa gattaaccta tctgcccatt ccccaaatgt ttaacgcggc gcttaacttc
    29761 ttcctgctta ccagcgttaa acggacgtgc atccgggcta cctaaatatc cgcacacgcg
    29821 gcgagttacc gacacacggg aggcgtcatg gttaccacat ttcgggcaag tgaagccttt
    29881 gctggtgcac tcgaactcac cggtaaagcc acactcgtag cactcatcaa tcggtgtatt
    29941 ggtgccgtaa tacggtacat gctgatagct gtaatcccag acatcttcca gcgctttcag
    30001 gttgtgctga atgtttggat actcgccgta gcaaatgaaa ccaccgttcg ccagcggcgg
    30061 gtaaggcgct tcaaagtcga tcttgtcgta cgggttcacc ttcttctcca catcaaggtg
    30121 gaaactgttg gtgtagtaac ctttatcggt cacgcccggc accacgccaa actcagcagt
    30181 atcgagacgg cagaagcggt cgcacaggtt ttcactcggc gtgctgtaga gactgaaacc
    30241 ataacccgtt tcttctttcc actgatccac tgcctgacgc agacgttcaa caatggcgat
    30301 acctttcgcg cgaagctgct cgttgtcgta aacatgctcg ccgccgaaca gcgcgttaat
    30361 ggtttcgtgg atgccgatgt aacccagcga aatagacgca cgaccgtttt tgaagatttc
    30421 agaaacatca tcgtcagcat tcagacgtac gccacaagca ccttccatat agaggatcgg
    30481 ggcgacgcgc gctttcacgc cttcgagacg agcgatacgg gtcatcagcg ccttacgtgc
    30541 cagcaccaga cgttcatcca gcagcttcca gaaggtggct tcatcgcctt ttgcttccag
    30601 agcaatacgc ggcaggttca ggctgatcac gccgaggttg ttacgaccat cgtggatctg
    30661 ctcgccgttt tcattttccc acacgccgag gaagctgcgg cagcccatcg gggttttaaa
    30721 cgaaccggtg actttcacta cctgatcgta gttcaggata tccggataca tgcgcttgct
    30781 tgcgcactcc agcgccagct gtttgatgtc gtagttcgga tcgccttttt tatggttcag
    30841 accatcgcga atcgcaaaca ccagtttcgg gaacaccgca gttttacggt ttttaccgag
    30901 gcctgcgata cggttacgca ggatcgattc ctgaatcagg cgcgattccc agctggtgcc
    30961 cagaccaaaa ccaaaggtta caaacggcgt ctgaccgttg gcggtgtgca gggtgtttac
    31021 ttcgtactcc agcgactgga aggcatcgta gcactctttg atggtacgag agtttgcgta
    31081 gccttcggcg tccgggatgt tccactcttc tgcggtcttg cgatgtttgt tgtagctggc
    31141 ggtaacaaac ggcgccagca cttcatcgat acggttaatg gtggtgccgc cataaatatg
    31201 gctggcgacc tgagcaataa tctgcgcagt taccgcggtt gccgtagaga tcgacttcgg
    31261 cggttcaatc tcggcgttcc ccatcttaaa gccctgggtc agcatgcctt tcaggtcgat
    31321 caacatgcag ttgaacattg ggaaaaacgg cgagtaatcg agatcgtgat agtgaatatc
    31381 gccacgctca tgtgccttca ccacgtcacg cggcagcagg tgctggcgtg catagtgttt
    31441 agccacgatc ccggccagca ggtcgcgctg ggttggaatc accttgctgt ctttgttggc
    31501 gttttcgttg agtaacgagg agttggtctg ctcgaccaga ccacggatct cctggttcag
    31561 gcgaccgcgt ttttcacgtt caatgtcgcg atcgtgacgg tactcgatgt aagcacgagc
    31621 cagttgtttg tatggacctg acatcagctg attttcaact gcggtctgga tctcattgat
    31681 atccacctgg ttgcggccct gcatctgctc gctgacaacc gcggcaacag tggcgcaata
    31741 atcggcatca tcgacttccg ctgctttagc tgcacgcaga atcgcttctt tgatgcgctc
    31801 tgatttaaac ggcactttgc agccgtctcg tttcatcaca tgcggtgtca tgatcgctcc
    31861 atatttttaa gaacaggtta tccacagaaa ttgggaaagg cgttttccgg ttttttcgtt
    31921 tcgttttccg gtgctttccg caatcgccat ccgcttatcc acaacgccgc acctttttcg
    31981 tggggagctg tagtagcaat tatagtcgat taatacaaca tattgggttt ggacgcattt
    32041 taaagtctat atatagtgct ttgcatcaag gatgttttaa ctttttttga tgtagctcaa
    32101 agtaaaaagc agagcgtacg gatgacgggc gctacagcgt tatgtaaatt ttttaatgaa
    32161 tttgctggtt gaaaaatcaa caaaaacaac atgctgacag acaaaacccc ggaatgaccg
    32221 gcattaccgg ggcttaggga agatttactt ctgtaaccac cagacagcct caaaaggccg
    32281 taaattcatg gcacagggtt gtggtgaggc ttcttcgtag ttatgcatca caagctgcca
    32341 gttgccgcgc atttgccctg gctgccaggg ttggatctca cggctaaggt tggcaatgac
    32401 cagcaaggtt tgccccttcc attcacggcg atagcaccac aatacagggc tgtttggcag
    32461 cagatcctgg taattgcccc atgtcaggat ggcttcttgc ttacgcagtg cgattaactt
    32521 ttggtaggtg taaaacaccg aggactcatc ggccagcgcg gcttctacgt tgattttttg
    32581 atagttatcg cccaggccaa tccacggttc gccagccgta aacccggcat tatcgccgtt
    32641 gctccattgc atgggcgtgc ggctgttgtc acgggattta ctggcgagga ttgccaataa
    32701 ctcgtcggca tcacgaccat cgttgcgcag ctcggcaaac atatttaggc tctccacgtc
    32761 gcgatagtca gtaatgcgcg tgaaatgcgg gttggtcatg ccaatctctt cgccctggta
    32821 gatatacggc gttccctgca tgccatgcag caccatcgcc agcatttttg ccgcaggcac
    32881 gcggtattca ccttcatcac caaagcgaga aacaatgcgc ggctgatcgt ggttacacca
    32941 gaacaaggca ttccatgcca cgttgtgcat tccttgttgc cagtggcgga acaatgtttt
    33001 caacgccaca aagtcaggtt tagccagcgt ccatttttca ccaccgggat aatcgacctt
    33061 caggtgatga aaattaaagg tcatcgacaa ttcactgcct gtcagtgccg cgtatcgctg
    33121 gcaatgctca aggctggtgg aggacatttc acctacggtc attaacccgc gtggcgtaaa
    33181 cacatcgcgg ttcatctcgt gcaaaaactc gtgtgctcgt ggcccgtcgg tgtagaagcg
    33241 acgcccgtcg ccatccaggt cttcagggaa acgcgggtct ttggagatca gattcaccac
    33301 atccaggcgc aacccgtcga ccccacgatc ggcccagaat tcacagactt ttttcagctc
    33361 tgcgcgtacc gctggattct cccagttgag atccgcctgt tctggtgcaa agagatgcaa
    33421 atagtactgt tcgctttccg catgccagcg ccacgcacta ccgccaaatt ttgaacgcca
    33481 gttgttcggt ggcgtttctg gttcgccatc gcgccagata taaaactggc ggtaagggct
    33541 ttctttgttc agcgcctcgc gaaaccaggc atgttgggta gaggtatggt taaacaccat
    33601 atcgagaatg atacgaatcc cgcgcgattt tgcctgcgtc accagttcgt caaaatcgtc
    33661 cagcgtgccg taggtgggat caatcgccgt atagttcgct acatcgtaac cgttatcgac
    33721 ctggggagag acataaaagg gggttagcca gatggcatca acgcccagtt tatgcagata
    33781 gtccaggcgt tggataacgc cacgtaaatc gccggtgccg ctacccgtgg tgtcctgaaa
    33841 actctttgga taaatctggt agataacgcc gttttgccac cagtggggaa gattagtcat
    33901 tacgttattc ctgcaaatgc gagggggcgc aattgcgccc cgaagaaaat taaacaatgt
    33961 ccagcgtgcc caggcggtat ttccgctgat agataaacga ggtgagtaca atcgggatga
    34021 tgatggcgat agccattgcc agcgcaaaca cctgccagta gctcggttga atcgagagaa
    34081 ttcccggcag gccgcctacg ccgataccat tcgccataac gccgttcagg ccgcatagca
    34141 atcctgccag accagaacca atcatcgcgc acagcatcgg gaagcgatat ttcaggttga
    34201 tgccgtacat tgcaggctca gtgaccccaa gccaggcgga gatagcggca ggcacggaga
    34261 tctcgcgctc attgtgcttg cggctggaaa tgataatgcc tattacggcg gagccctgag
    34321 cgatattcga cagcgcaatc agcggccaca ctggcgtgcc acccatgctt tgaatcatct
    34381 gcaaatcaat agcaagggtg gtctggtgta cgccggtgat caccagcggt gcgtacagga
    34441 agccaaacaa tgccgcacca atcggagcaa agctgccggt catcaggtga cgtaccgcaa
    34501 aggcaacgcc atcgccaatc atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca
    34561 ccgcgaggat cagcgaacag acgggcacca ccaccagata gaggtaatcc ggcacgatgc
    34621 gtttaaggcg agtttcaata acgcccagcg ccagcccggc taacagtgcc gggatcacct
    34681 gcgcctgata gcctactttg gcgatgctga acatgccaaa gtcccacact tccggcagct
    34741 gctgcccgag cagataagcg ttcatcagct ggggagaaac cagtgtcacg ccaagcacga
    34801 taccaaggat cggcgtgccg cccatttttt tcaccgctga ccagcaaatc ccgaccggca
    34861 ggtagaagaa gatcgcttca ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag
    34921 ggtacatttg cgccagcgtc tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa
    34981 aaccgaggat caaaccgccg ctaatcaacg cgggcagcag cgggaagaag atctccgcga
    35041 aatgagagat caattgctca tgccatttca tattctgccg ggcggctttt tttacctgct
    35101 ctttatcaac ctgcgcctgt ccggttgacg caatcagtgc ttgatagtaa tcacccacat
    35161 tggtgccaat caccacctga aattgcccgg cattggtgaa acagcctttc accatgggga
    35221 gttgctcaat ttctttcggt ctggcattgg ccggttggtt gaggacaaag cgcaggcgag
    35281 taatacagtg gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca atcaaccgat
    35341 cgatatccgt ttggtttatt ttgctcatca taaagcccca tggcagatga catttttggt
    35401 tggctgcaga atatattgcg cggaaaaatt taaaaacggg aacgttcccg aaacgcagcg
    35461 aagatcacaa tttatcgttc aggaaacgat caggacaggg tggcggggat gatgatttgt
    35521 tgcggttcgc tgcgcccggt tacctgtgcg atcaattggc aggccgcctg gcgtccagct
    35581 tcggcgtaac caggatctac ggtgacgatc tccggatgaa ggaatttcat taatggcgta
    35641 ttaccgacgc tcgccagttg caaggtgtcg atgcgttgct cttgcaggta tttacttgcg
    35701 ccaagtgcca gcgtgtcggt tgcgcacagt aaggcggtag tttcaggcgt aatcactttt
    35761 gccacgttct catagccttg cttcatagca aggcctggca gagcggcaac gggattcagt
    35821 ttatgcgctt tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt cgtcacgtca
    35881 ctatgcggca cgccgagata actgatatta cgatgcccct ggtcatacag ccgttgcatc
    35941 aggatcttga ttgccccttc gtcgtcataa cagaccgaag caaaaccttt tgcgtcacgc
    36001 gccagcagaa ccagcgatga ctgccagtgg gctaacattt cttctgttat gccagtaaaa
    36061 ccgaacagca ctacgccgtc gatattacgc cgtttcagca cccccaaatg ttcggcaact
    36121 aattgcgggg aaaactgact ttccatcatg attgggtcgt aaccttgttc atagaacgct
    36181 ggcagcatgg tttgaacggc gagattttct gacaacgaat ccaggcgggt aacaatgatg
    36241 gcgaccactt tatcgctttg cccacgcata gcgcgcgcag agcgggaagg ggaaaatcca
    36301 tgctgattca tcactgcttc aacacgctcg cgggtgcgct ggctcacgcc gctttcgtta
    36361 ttcagcaccc gggaaactgt agatttcccc acgccgctta agcgtgcgat gtctttgatg
    36421 gtcagccgat tttgcatcct gttgtcctgt aacgtgttgt ttaattattt gagcctaact
    36481 ttacccgtgc attcagcaat gggtaaagtc tggtttatcg ttggtttagt tgccagcagg
    36541 tattatatcg ccatagatgc tacgaatatt attggattct ccttattatt tgcggcgctt
    36601 ttttcactta ccggaggtta tatggaacct gatcccacgc ctctccctcg acggagatta
    36661 aaacttttcc ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt
    36721 ttaacgtccc tgctcagctt tattaccttc aggtaaggct tcgccacgcc tgaagacatt
    36781 tctgtactgt ttcagacagt gcggagggac tccttatgtt taaagaaatt tttacccggc
    36841 tcattcgcca tttaccttcc cgtctggttc atcgcgatcc attgcctggc gcgcagcaga
    36901 cagtgaatac ggcggtcccg ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc
    36961 ccgaagaaga attatggaaa acgttcgaca cccatccgga agggttaaat caggcggaag
    37021 tggaatctgc ccgcgaacaa catggtgaaa ataaattacc cgcacaacaa ccgtcgccgt
    37081 ggtgggtaca tttatgggtc tgctatcgca acccctttaa tattttactc accattctcg
    37141 gcgctatttc ttacgccacg gaagatttat ttgccgcggg cgttattgcg ctaatggtcg
    37201 ctatttcaac gttactgaac tttattcagg aagcacgttc cactaaagcg gcagatgccc
    37261 tgaaagcgat ggtcagcaat accgcgacgg tgctgcgcgt aattaacgac aaaggcgaaa
    37321 atggctggct ggagatcccg atcgaccagc tggtgcccgg cgatattata aaactggcgg
    37381 cgggagatat gatcccggca gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc
    37441 aggcgtcgtt aaccggtgag tctctgcccg tggaaaaagc cgctaccact cgccagccgg
    37501 agcacagcaa tccgctggag tgcgacacgc tgtgttttat gggcaccacc gtggtgagcg
    37561 gcacggcaca agcaatggtg attgctacag gtgccaatac ctggtttggt caactggcgg
    37621 ggcgtgttag tgagcaggaa agcgagccga atgcctttca gcaggggatc agccgcgtca
    37681 gtatgctgct gattcgcttt atgctggtga tggctccggt ggtgttgctt atcaacggtt
    37741 acaccaaagg cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg gtaggcttaa
    37801 cgccggaaat gttgccgatg attgttacct cgacgctggc gcgcggcgca gtaaagctgt
    37861 cgaaacagaa agtgatcgtc aaacatctgg atgctattca gaactttggc gcaatggata
    37921 ttctgtgcac tgataaaact ggcaccctga cacaggacaa aattgtgctg gagaatcata
    37981 ccgatatctc cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg aacagtcatt
    38041 atcagaccgg acttaaaaac ctgctcgata cagcggtgct cgaaggtacg gatgaagagt
    38101 cagcgcgctc gctggccagt cgttggcaaa aaattgatga gattccgttt gatttcgagc
    38161 gtcgccggat gtcggtggta gtggcagaaa ataccgagca ccatcagctg gtttgcaaag
    38221 gtgcattgca ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtgc
    38281 cgctcgatga catcatgctg cgtaagatta agcgggttac tgatacgctt aatcgtcagg
    38341 ggctgcgcgt ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga gattaccagc
    38401 gggcggatga atccgacctg atcctcgaag gatatattgc tttccttgat ccgccaaaag
    38461 agacaaccgc tccggcactg aaggcattaa aagcgagtgg aatcaccgta aaaatcctta
    38521 ctggcgacag tgagttagtt gcagcgaaag tgtgccatga agtggggctg gacgcgggag
    38581 aggtggtcat tggtagtgat attgaaacgc tatctgacga cgaactggca aatctcgcac
    38641 agcgcaccac gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt gtgaccttac
    38701 tgaagcgcga agggcatgtg gttggcttta tgggcgatgg tattaatgat gcgcccgcct
    38761 tacgcgctgc ggatatcggc atttctgtgg acggcgcggt agatattgcc cgtgaagcgg
    38821 ctgatatcat cctgctggaa aaaagcctga tggtgctgga agagggggtt attgaaggac
    38881 gtcgcacttt cgccaacatg ctgaaataca tcaaaatgac ggcgagctcc aacttcggta
    38941 atgtgttcag cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg ttgccgttac
    39001 acttgcttat tcagaacctg ctgtatgatg tgtcacaggt ggcgatcccg tttgataacg
    39061 tcgacgacga gcaaattcaa aagccgcagc gttggaatcc ggcggatctg gggcgcttta
    39121 tgatcttctt cggaccgatc agctcgatct tcgatattct gacgttttgc ctgatgtggt
    39181 gggtattcca tgccaacacg ccggaaacgc aaacgctgtt ccagtcggga tggtttgtgg
    39241 tgggcttact gtcgcaaacg ctgattgtgc atatgatccg tacccgccgt gtgccgttta
    39301 ttcagagctg tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg attgtcggga
    39361 tcgcattgcc gttttcaccg ctggccagtt atctgcaatt acaggcgctg ccgttaagct
    39421 atttcccgtg gctggttgcg attctggcag ggtatatgac gttaacccag ttggtgaaag
    39481 ggttctatag ccgtcgttac ggctggcagt aaagaataaa ctgggcacga tagccccgat
    39541 tcagattgca gacaaagtga gcgttgttca tgccggatgc ggcgtaaatg ccttatccgg
    39601 cctacaaatt caatcaattg cagga
//
LOCUS       DAPPNJ010000017        37999 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000017 DAPPNJ010000000
VERSION     DAPPNJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.017"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            163..1527
                     /locus_tag="RPM73_000662"
     CDS             163..1527
                     /locus_tag="RPM73_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519054.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1655..2146)
                     /gene="yajQ"
                     /locus_tag="RPM73_000663"
     CDS             complement(1655..2146)
                     /gene="yajQ"
                     /locus_tag="RPM73_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDX6519055.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2314..3225
                     /gene="panE"
                     /locus_tag="RPM73_000664"
     CDS             2314..3225
                     /gene="panE"
                     /locus_tag="RPM73_000664"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDX6519056.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3188..3778
                     /gene="yajL"
                     /locus_tag="RPM73_000665"
     CDS             3188..3778
                     /gene="yajL"
                     /locus_tag="RPM73_000665"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDX6519057.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3832..5280)
                     /gene="thiI"
                     /locus_tag="RPM73_000666"
     CDS             complement(3832..5280)
                     /gene="thiI"
                     /locus_tag="RPM73_000666"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDX6519058.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5486..5728
                     /gene="xseB"
                     /locus_tag="RPM73_000667"
     CDS             5486..5728
                     /gene="xseB"
                     /locus_tag="RPM73_000667"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDX6519059.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQDKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5728..6627
                     /gene="ispA"
                     /locus_tag="RPM73_000668"
     CDS             5728..6627
                     /gene="ispA"
                     /locus_tag="RPM73_000668"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDX6519060.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6652..8514
                     /gene="dxs"
                     /locus_tag="RPM73_000669"
     CDS             6652..8514
                     /gene="dxs"
                     /locus_tag="RPM73_000669"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDX6519061.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8569..9543
                     /gene="yajO"
                     /locus_tag="RPM73_000670"
     CDS             8569..9543
                     /gene="yajO"
                     /locus_tag="RPM73_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDX6519062.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDGSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(9597..10115)
                     /gene="pgpA"
                     /locus_tag="RPM73_000671"
     CDS             complement(9597..10115)
                     /gene="pgpA"
                     /locus_tag="RPM73_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDX6519063.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10093..11070)
                     /gene="thiL"
                     /locus_tag="RPM73_000672"
     CDS             complement(10093..11070)
                     /gene="thiL"
                     /locus_tag="RPM73_000672"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDX6519064.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11148..11567)
                     /gene="nusB"
                     /locus_tag="RPM73_000673"
     CDS             complement(11148..11567)
                     /gene="nusB"
                     /locus_tag="RPM73_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDX6519065.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11587..12057)
                     /gene="ribE"
                     /locus_tag="RPM73_000674"
     CDS             complement(11587..12057)
                     /gene="ribE"
                     /locus_tag="RPM73_000674"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDX6519066.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12146..13249)
                     /gene="ribD"
                     /locus_tag="RPM73_000675"
     CDS             complement(12146..13249)
                     /gene="ribD"
                     /locus_tag="RPM73_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDX6519067.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13253..13702)
                     /gene="nrdR"
                     /locus_tag="RPM73_000676"
     CDS             complement(13253..13702)
                     /gene="nrdR"
                     /locus_tag="RPM73_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDX6519068.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13853..14392
                     /locus_tag="RPM73_000677"
     CDS             13853..14392
                     /locus_tag="RPM73_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDX6519069.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14691..15575
                     /gene="tsx"
                     /locus_tag="RPM73_000678"
     CDS             14691..15575
                     /gene="tsx"
                     /locus_tag="RPM73_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDX6519070.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15752..16099)
                     /locus_tag="RPM73_000679"
     CDS             complement(15752..16099)
                     /locus_tag="RPM73_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDX6519071.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16228..17199)
                     /gene="secF"
                     /locus_tag="RPM73_000680"
     CDS             complement(16228..17199)
                     /gene="secF"
                     /locus_tag="RPM73_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDX6519072.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17210..19057)
                     /gene="secD"
                     /locus_tag="RPM73_000681"
     CDS             complement(17210..19057)
                     /gene="secD"
                     /locus_tag="RPM73_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDX6519073.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19085..19417)
                     /gene="yajC"
                     /locus_tag="RPM73_000682"
     CDS             complement(19085..19417)
                     /gene="yajC"
                     /locus_tag="RPM73_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDX6519074.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19440..20567)
                     /gene="tgt"
                     /locus_tag="RPM73_000683"
     CDS             complement(19440..20567)
                     /gene="tgt"
                     /locus_tag="RPM73_000683"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDX6519075.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20623..21693)
                     /gene="queA"
                     /locus_tag="RPM73_000684"
     CDS             complement(20623..21693)
                     /gene="queA"
                     /locus_tag="RPM73_000684"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDX6519076.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21786..22367
                     /gene="acpH"
                     /locus_tag="RPM73_000685"
     CDS             21786..22367
                     /gene="acpH"
                     /locus_tag="RPM73_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDX6519077.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22372..24189)
                     /gene="malZ"
                     /locus_tag="RPM73_000686"
     CDS             complement(22372..24189)
                     /gene="malZ"
                     /locus_tag="RPM73_000686"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDX6519078.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(24345..25718)
                     /gene="proY"
                     /locus_tag="RPM73_000687"
     CDS             complement(24345..25718)
                     /gene="proY"
                     /locus_tag="RPM73_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDX6519079.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25794..27113)
                     /gene="brnQ"
                     /locus_tag="RPM73_000688"
     CDS             complement(25794..27113)
                     /gene="brnQ"
                     /locus_tag="RPM73_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDX6519080.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27520..28815)
                     /gene="phoR"
                     /locus_tag="RPM73_000689"
     CDS             complement(27520..28815)
                     /gene="phoR"
                     /locus_tag="RPM73_000689"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDX6519081.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28873..29562)
                     /gene="phoB"
                     /locus_tag="RPM73_000690"
     CDS             complement(28873..29562)
                     /gene="phoB"
                     /locus_tag="RPM73_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDX6519082.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29752..30954
                     /gene="sbcD"
                     /locus_tag="RPM73_000691"
     CDS             29752..30954
                     /gene="sbcD"
                     /locus_tag="RPM73_000691"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDX6519083.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQQYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30951..34094
                     /gene="sbcC"
                     /locus_tag="RPM73_000692"
     CDS             30951..34094
                     /gene="sbcC"
                     /locus_tag="RPM73_000692"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDX6519084.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34220..35404
                     /gene="araJ"
                     /locus_tag="RPM73_000693"
     CDS             34220..35404
                     /gene="araJ"
                     /locus_tag="RPM73_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDX6519085.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(35547..36455)
                     /gene="mak"
                     /locus_tag="RPM73_000694"
     CDS             complement(35547..36455)
                     /gene="mak"
                     /locus_tag="RPM73_000694"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDX6519086.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36580..37491
                     /gene="rdgC"
                     /locus_tag="RPM73_000695"
     CDS             36580..37491
                     /gene="rdgC"
                     /locus_tag="RPM73_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDX6519087.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37569..37652)
                     /locus_tag="RPM73_000696"
     CDS             complement(37569..37652)
                     /locus_tag="RPM73_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519088.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37649..>37999)
                     /locus_tag="RPM73_000697"
     CDS             complement(37649..>37999)
                     /locus_tag="RPM73_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HDX6519089.1"
                     /translation="RSPDIVAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPA
                     SLLTTLTESRHRSLAMNNPQLAADVKTTWLKEDPSLLLFVEQPDLSLLRDLVKTGAMR
                     KIRSEARHWLEEKQ"
BASE COUNT         9383 a        10225 c         9666 g         8725 t
ORIGIN      
        1 taaaattcat tttttcaaca tatagagcat tcattttatg aatgcaaaaa aacatacctg
       61 acacacaaat tactctcctc tgtaatattt tctcattaac actctgttta cccaacatgc
      121 gctccgcact acactatgtg ggcatttttt taaggggtgg taatgaacga ttataaaatg
      181 acgccaggtg agaggcgcgc gacctggggt ttagggaccg tattctcgtt gcgcatgctg
      241 ggcatgttta tggttctgcc ggttctgacc acgtacggca tggctctgca aggtgccagc
      301 gaagcattaa tcggtattgc cattggtatt tatggtctga ctcaggccgt ttttcagatt
      361 ccgtttggcc tgctttccga ccgcattggt cgcaaaccat taattgtcgg tgggctggcg
      421 gtgtttgccg ccggtagcgt tatcgctgcg ctttccgact ccatctgggg aattattctg
      481 ggccgggcgc tacaaggctc tggagcaatt gccgctgccg ttatggcgct gctttccgat
      541 ctcacgcgcg aacaaaaccg taccaaagca atggcgttta tcggcgtgag ctttggcatt
      601 acctttgcca ttgcgatggt gcttggcccg atcatcactc acaaacttgg gctgcacgcg
      661 ctgttctgga tgatcgctat tctggcaaca accggcattg cgttgaccat ttgggttgtg
      721 cccaacagta gcactcacgt acttaatcgt gagtccggaa tggtgaaagg cagtttcagt
      781 aaagtgctgg cggaaccgcg gctgctaaaa ctcaactttg gcattatgtg tctgcacatg
      841 cttctgatgt ctacgtttgt tgccctgccc ggacagctag ctgatgcggg gttcccggcg
      901 gctgaacact ggaaggtcta tctggcgacg atgttaatcg cctttggctc ggtcgtgcct
      961 ttcattatct acgctgaagt taagcgcaaa atgaagcaag tctttgtctt ctgcgtcggg
     1021 ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa cgcagttctg gcaactggtg
     1081 gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg aagcccttct gccctcactt
     1141 atcagtaaag agtcgccagc aggttacaaa ggtacggcga tgggtgtcta ctccaccagc
     1201 cagtttcttg gcgtggcgat tggcggttcg ctgggcggct ggattgacgg catgtttgac
     1261 ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag tgtggctggc agtcgccagt
     1321 accatgaaag aaccgccgta tgtcagcagt ttgcgcattg aaatcccggc ggacattgcc
     1381 gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag gcgtcaaaga agtgttgatt
     1441 gcagaagaag agcattcagc ttatgtgaaa atcgacagca aagtgacaaa tcgctttgag
     1501 gttgaacagg caattcgtca ggcataaaaa acgcggcgaa ttgcctgctg cgctacgctt
     1561 accaggccta caaagttctt gagttacaaa cctttgtagg ccgaatcagg cgttcatgcc
     1621 gcatctgaca tgaacaaaac gcacatagtc gcgattaatc gcggaagttt ttgaactgga
     1681 acggctgacc gagatcgcca ccacgtacca tcgccatgac agcctgcaaa tcatcacgag
     1741 atttgcccgt tacgcggatt tcatcgccct gaatttgcgc ctgcactttc agtttgctgt
     1801 ctttgatcat cttgacgatt ttcttctggg tcgcgctctc aatgccctgt ttcagtttcg
     1861 cttccacaaa ccaggtttta ccgctatgaa cgatattttc cggtacatcc agtgaactgc
     1921 cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga ttgacctgga
     1981 agtcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca aatgaggctt
     2041 caacgttacg gaagtcaaaa cgggactcca cttcgcgact cgcgttatcg accgcgttac
     2101 gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt tcctctccct
     2161 tcatttttaa tgcgaagcat aatacctgca aagttaaata accaacagtc ctgcaccagg
     2221 acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat agccttattg
     2281 aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt gggatgcggt
     2341 gccttagggc aattatggct tacagcactt tgtaaacagg gtcacgaagt tcagggctgg
     2401 ctgcgcgtac cgcaacctta ttgtagtgtg aatctggttg agactgatgg ttcgatattt
     2461 aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca ccagcgatct gctcctggtg
     2521 acgctgaaag catggcaggt ttacgatgcc gtcaaaagcc tcgcgtccac actgcctgta
     2581 accacgccaa tactgttaat tcacaacggc atgggcacca tcgaagagtt gcaaaacatt
     2641 cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg caatgtcatt
     2701 attcatgtgg caaacggaat cacgcatatt ggtccggcac ggcaacagga tggcgattac
     2761 agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca taacaatatt
     2821 cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga ttaatccact gaccgccatc
     2881 tggaattgcc cgaatggtga attacgtcat catccgcaag aaattatgca gatatgcgaa
     2941 gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt cagcagaaga tttgcgtgat
     3001 tacgtgatgc aggtgattga tgccacagcg gaaaatatct cgtcgatgtt gcaggatatc
     3061 cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg ccgcgcccgc
     3121 gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa aagaaaggag
     3181 agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag agactgaagc
     3241 cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa gtcaccactg ccagcgtcgc
     3301 cagcgatggc aacctggcca ttacctgctc gcgcggcgtg aagctgctgg cggatgcgcc
     3361 gctggtcgag gtggctgatg gcgaatatga cgtgatcgta ctgcctggtg gcattaaagg
     3421 cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc gttaaacagt tccaccgttc
     3481 cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc cgcacgatat
     3541 cttcccgatt ggtaatatga ccggcttccc gacgctgaaa gacaaaattc ccgctgaaca
     3601 atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca gccaggggcc
     3661 ggggacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc gcgagaaagc
     3721 ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt acgagtagtg
     3781 tcggatgcgg taagcgtcgc atctgaccag atgcgacgtt acaaccacaa attacgggcg
     3841 atataccttc acattgttaa agccctgctc gcgcagatag agcgcctgca gacggctcat
     3901 caccccgcgc tcacaccaca gtagccaggt tctgttctgg tcgagatcgc caaacttggt
     3961 gctcagttta tagaacggca gagaaactac atcaatccct tcgactttca gtggcttatc
     4021 ttcctgttca tcgatagaac ggatatcgag gatcacgtcg ttcgggccga agccattgac
     4081 ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga tatcaacgtt
     4141 attcgcttct tcaaccactt tatcgagaat gctgaagtcg aatttctctt cttccgcttc
     4201 aatcttcgac ttaaccgctt tcaccgtcgg gcttttggag ataacaccgc aatattccgg
     4261 catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga tgtgctcttt
     4321 gtcgtaagaa atcagcggac gcaggatcag cgtatcggag acgttatcaa tcaggcgcag
     4381 gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca gcgcctgtac
     4441 gccgtaacgt tcagccactt tagacgcggc acgcaccatc atacgtttga ggataacgcc
     4501 catctgaccg tcgtcgattt tctcgagaat ttccccgacg accggttcga aattaatagc
     4561 gacaaaacgc acgcggtggg agctgccaaa acggttccac agataatgcg ccacctgacg
     4621 cacgccaatt tcatgcgccg cgccgccaag gttaaagaaa cagtaatgca cgcggcagcc
     4681 gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa tgagcgacag
     4741 cacatcttcc tgggtgccga tcgggaaacc gccaatacct tcgtagcggc ctttaatcag
     4801 caggagacga tcgtcttcca cttccagatg gacagtcact tccggattgg tcagcttcac
     4861 gcgcgcggac tcaatatgct gatttaaacc gccgccgacg taacgttcca catcaatcga
     4921 gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc cttccagctg
     4981 atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa acggcacgtc
     5041 ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc gaatagccag
     5101 acgctggttt tcatcttttg cgcgaacttc gatgttatcc cagtggcgga cgacagcgag
     5161 cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt ttataaagcg
     5221 caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga taaacttcat
     5281 ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat acagcagccc
     5341 acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg ctgcgcacat
     5401 caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg acggggcgaa
     5461 aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggctcccg ccagctttga
     5521 aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg acctgccgct
     5581 ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg ggcaggacaa
     5641 attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag acgcctctct
     5701 aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga agcctgcgtt
     5761 aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca gaacactccc
     5821 gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg accttttctg
     5881 gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc acccgctgcc
     5941 gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc gatggatgat
     6001 gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc aaacgcgatt
     6061 ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc tgatatgccg
     6121 gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc cagcggtatt
     6181 gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca cgtacctctg
     6241 gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc cgccgttcgc
     6301 cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact cgacaagtac
     6361 gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt ggtaggagat
     6421 actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag tacctaccct
     6481 gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga cgatgcccgc
     6541 cagtcgctga aacaactggc tgaacaatca ctcgatacct cggcactgga agcgctagcg
     6601 gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct gatgagtttt
     6661 gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt acgactgttg
     6721 ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact cgacagcgtg
     6781 agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac cgtggcgctg
     6841 cactatgtct acaacacccc gtttgaccaa ttgatttggg atgtggggca tcaggcttat
     6901 ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca gaaaggcggc
     6961 ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt cgggcattca
     7021 tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga aggcaaaaat
     7081 cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg ctggcatggc gtttgaagcg
     7141 atgaatcacg cgggcgatat ccgtcctgat atgctggtgg tcctcaacga caatgaaatg
     7201 tcgatttccg aaaatgtcgg cgcgctcaat aaccatctgg cacagctgct ttccggtaag
     7261 ctttactctt cgctgcgcga aggcgggaaa aaagttttct ctggcgttcc gcccattaaa
     7321 gagctgctca aacgtaccga agaacatatt aaaggcatgg tagtgcctgg cacgttgttt
     7381 gaagagctgg gctttaacta catcggcccg gttgacggtc acgatgtgct ggggcttatc
     7441 accacgctga agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat catgaccaaa
     7501 aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca ctttccacgc cgtgcctaaa
     7561 tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag ctattcaaaa
     7621 atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat ggcgattact
     7681 ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc ggatcgttac
     7741 ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg ccgccggtct ggcgattggt
     7801 gggtacaaac ccattgtcgc gatttactcc actttcctgc aacgcgccta tgatcaggtg
     7861 ctgcatgacg tggcgattca aaagctcccg gtcctgttcg ccatcgaccg cgcgggcatt
     7921 gttggtgctg acggtcaaac ccatcagggc gcttttgacc tctcttacct gcgctgtata
     7981 ccggaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat gctctatacc
     8041 ggctatcact ataacgacgg cccgtccgcg gtgcgctacc cgcgcggtaa cgcggttggc
     8101 gtggaactga cgccgctgga aaaactgcca attggcaaag gcattgtgaa gcgtcgtggc
     8161 gagaaactgg cgatccttaa ctttggtacg ctgatgccag acgcggcgaa agtcgctgaa
     8221 tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac cgcttgatga agcgttaatt
     8281 ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc cattatgggc
     8341 ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc gtaaaccagt acccgtgctg
     8401 aacattggcc tgcctgactt ctttattcca caaggaactc aggaagaaat gcgcgccgaa
     8461 ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc ataatcccta
     8521 ctccactcct gctatgctta agaaattatt catggcagga gtggcagcat gcaatacaac
     8581 cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat gacctttggc
     8641 gagccagatc gtggtaatca cgcatggaca ctgccggaag agagtagccg tcccatcatt
     8701 aaacgcgcgc tggaaggcgg cataaatttc tttgataccg ccaatagcta ttccgatggc
     8761 agcagcgaag agatcgttgg tcgcgcactg cgggatttcg cccgtcgtga agacgtggtc
     8821 gttgccacca aagtgttcca tcgcgttggt gatttaccgg aaggattatc ccgtgcacaa
     8881 attttgcgct ctatcgacgg cagcctgcga cgtctcggca tggattatgt cgatatcctg
     8941 caaattcatc gctgggatta caacacgccg atcgaagaga cgctggaagc tctgaacgac
     9001 gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc ttcgcagttt
     9061 gctcaggccc tgaagctaca aaaacagcac ggctgggcgc agtttgtcag tatgcaggat
     9121 cactacaatc tgatttatcg cgaagaagag cgcgagatgc tgccactgtg ttatcaggag
     9181 ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc gactgacgcg tccgtgggga
     9241 gaaactaccg cacgactggt gtctgatgag gtggggaaaa atctctataa agaaagcgat
     9301 gaaaatgacg cgcagatcgc agagcggtta acgggcgtca gtgaagaact cggtgcaaca
     9361 cgagcacaag ttgcgctggc ctggttgttg agtaaaccgg gcattgccgc accgattatc
     9421 ggtacatcgc gggaagaaca gcttgatgag ctattgaacg cggtggatat cactttgaag
     9481 ccggaacaga ttgccgaact ggaaacgccg tataaaccgc atccggtagt aggatttaaa
     9541 taagagtcca ggcctgatga gacgcaacaa gcgtcacatc aggcatcggt gcacaactac
     9601 gacagaatgc ccagcggcca gtgatgacca ataaagtaca ggatacctgc ggagatcacc
     9661 ccggcgacaa tatcatctat catgatcccc atgccgccat gcacattgcg atcaaaccag
     9721 cggatcggcc acggtttcca catatccaga atacggaaaa tcacaaaccc ggcggcaacc
     9781 cactgccagt cattggtcgg cagcgccatg agcgtgatcc acataccaat aaattcgtcc
     9841 cagacaatgc tgccgtgatc gtgcacgccc atatcttttg cggtctgatg acaaagatag
     9901 acgccgatac agatccccag catcaccacc agcgagtaga gctgccaggg caaaaaggtc
     9961 atcagatacc agaacggaat cgctgccagc gagcccatcg tcccaggaac gatcgggctt
    10021 aatccacttc cgaatccgac agcaagcaga tgccacggat tactcatctt caggcgactt
    10081 ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct ttccagtcaa acgtgacagg
    10141 ttcgccgtca cgaataaaac aaagcccttc gatatcggcg gtcatttgcc cgatacaggt
    10201 aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg ccacggttca gttccggcac
    10261 cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg cctgttccgg
    10321 ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa tacgtgcgcc
    10381 gcagtcgctg gctttcacga tatgcccgag atcggaaatc agaccgtcag agagatcgat
    10441 agctgaattt gccagatcgc gcagtgcctg cccctgtaaa atacgcggcg atggacggag
    10501 atgacgtttg atcaagtagt cagcatcttt agcatcggca acctgcaaac ggttttgcaa
    10561 aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc agtcacccgg
    10621 tttcgcccca gagcgcgtta aggctcgtcc catcggaaca aagccgtgga tacccaacgt
    10681 cattgataat ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt aataattgag
    10741 aagatcaaac aaactgtcgc tgaaggactc aagccacgct tcgtctacgt ccggtaaggt
    10801 taatgccagc gtcagccagg ccggatcggc ccccatcgct gccagatcgc ttaggttcac
    10861 cgccagtgct ttatacgcca gatcagcagg atcgatatca tggaggaagt ggttgcccgc
    10921 caccagcgta tcagtgctga tcgccagggt ctgtttctcg gggatattga gaagagcgca
    10981 atcgtcgccg atgcccagtt cgacatcaag acgagaactt cttacacggt caaaataacg
    11041 ggcaatcagg gagaactcgc cacatgccat acgttatgcc tcagcaggta aaaaagaaaa
    11101 ggccggcgat cgcggaacgg tcttccgtga atctaccggc ctggatatca ctttttgtta
    11161 gggcgaatca caggtgctgc tttatcgagt acgccgttga cgaacttatg gctgtcttct
    11221 gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg ccactttgta tggcacatcg
    11281 ctacgtttag acagctcgta cagcgcaatg cgcagtactg ctttttctac ctgtcccagt
    11341 tcttccagca ggcgggacag gtatggcttc atcagtccgt cgaggtatgc ggtgttagtc
    11401 gccaccccgg ccagcagctc acggaagtac aggacgtcaa cgtcttttac atcctgttca
    11461 gccaggaact ggtattcaac atcagcgatg tcgttctggg acaactgcca ggagtagagc
    11521 gcctggacgg cacactcacg agcgcggcga cgagcagcag gtttcacgga tttcccctta
    11581 ctaatttcag gccttgatgg ctttcaatac attaatcatt tcaagcgcgg tcagtgcagc
    11641 ttctgcacct ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt caatgctttc
    11701 agtggtcaga accccaaaag caaccggaat ttcgctgtcc tgggcaacat gcgccaggcc
    11761 gttgcttgca ccaccagcga catattcaaa gtgggcagtg ccaccacgaa taaccgtacc
    11821 cagcgcaatc accgcgtcgt atttaccggt tttagccagt gcacccgccg ccagcggcag
    11881 ctcataggca ccaggcaccc aaacaacggt aatgttttca tcttttacct gaccgatacg
    11941 tttcagtgcg tcaattgcac cttccagcag gctgtcattg ataaagttgt tgaaacgcgc
    12001 gatggtgatg gcgacgcgag cgtccggggt agcaacgtta gcttcaataa tgttcatttt
    12061 cttccttcgg gttcgagtat ggccccgcag gggggcggat tttagcataa tatttcgtgc
    12121 gctgcttccc tttcgagccg ggagatcatg cacccactaa atgcaggcaa acatccgggc
    12181 ctacatgacg tatctctttg aatttaaatt ggggggcgtc ggctaatttc tcaagccctg
    12241 gcagcgtgca taatccgcgg gcgtcgctgc ctaatagttt aggtgcgata tagacaatca
    12301 gctcatcgac taaacccgcc tgcagcaatg cgccagcgag cgttggcccc gcttccaccc
    12361 agatgctgtt aatttgctgt ttacccagtt gcatcatcag tacaaccaga tccagatgac
    12421 ctttatgctc tggaatcagc aaggtacgca ccgtttccgg ccactcacta gaatcttcct
    12481 gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc ggcgtcacgc
    12541 gattttggct atcaatcaca atacgtactg gctggcggag attttgttgc gggtagagcg
    12601 cctgagtttg ttcatccagt tcagaccaac gcactgttaa ggctggatca tccgccagca
    12661 ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg ctgtcgttgt acgtcgcgcc
    12721 gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc catcgccgtg cggccatcaa
    12781 gcgatgcgcc aagttttaac tgaatataag gaaagccggt gcgcatccgc ttgagaaagc
    12841 ctttattcaa ttgctcggct tcactcatca tcagcccgtg gctgacgtca atgccagcct
    12901 gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc atcgaggcaa
    12961 ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca cggtggcgta cgaccatgat
    13021 ggctacaggg ttcgagtgtg acataggcgg tcgcaccttt ggctttttca cccgccatac
    13081 gcaacgcgtg tacttcggca tgtggttcac ccgcacgttg gtggtaacct tcaccaacaa
    13141 tttcgccatc tttgacaatg acgcacccga cattcgggtt gggatgcgtg gtaaaacgtc
    13201 ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc gtcctgcacg gcttagtcct
    13261 ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc gaaactgcgg tagacagagg
    13321 caaaacggat ataggcgact ttatcgagct ttttcaattg ctccatcacc agattgccaa
    13381 tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag ctgcgattta atatgattga
    13441 ttgccatttc gacgtcatcg gaactcaccg gacgtttttc cagcgcccgc agcattccgc
    13501 tacgcaattt ctcttcatta aacggttcac gcacgtcgtt gctttttaca acacgcggca
    13561 taaccagctc cgccacttca aaggtggtga aacgttcatt acacaccaga cactgccgac
    13621 ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc aattacctta gtgtccacgg
    13681 cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt ggttaacaga aatctatttt
    13741 accctgaact gacgatacaa caaaggcgca acgcttttga gataaaggat cgatgctttc
    13801 gctgctttta ccctctacca ttaggctaat tgcgacgaga aagggacgac ctatgaatac
    13861 taacgttttt cggctgctcc tgctgggaag cctgttcagc cttagcgcct gtgtgcagca
    13921 gagcgaagtg cgacagatga aacacagcgt cagcacgctg aaccaggaga tgacgcaact
    13981 caataaagaa acggtcaaaa tcactcagca aaacaggctg aatgcaaaat ccagcagtgg
    14041 ggtttacctt ctgcctggag cgaaaacacc ggcaagactg gaaagccaga tcggtacttt
    14101 acgtatgtcg ctggtgaata ttacgcctga tgcagatggc accaccctga cgctacgtat
    14161 tcagggcgag tcgaatgatc ccttgcccgc attcagcgga actgttgaat atgggcaaat
    14221 tcagggaaca atagacaact ttcaggaaat caatgtgcag aatcaattga ttaatgcccc
    14281 tgccagcgtc ctcgccccca gcgatgttga tattccgtta cagttaaagg gtatttctgt
    14341 agaacagtta gacttcgtac gtatccacga tattcaacct gttatgcagt aaacgtattt
    14401 cggggcgatt ttgtgcgtcc cgcaacatct ttccccgtca ttttgttact ccgcttacat
    14461 cacccagatt gatagtaaaa gtttgcaaca agggtgaaag tcagtacaat ccctgcccga
    14521 atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata gaactgtgaa acgaaacata
    14581 tttttgtgag caatgatttt tataataggc tcctctgtat acgaaatatt tagaaacgca
    14641 atttgcgcct ttttcactcc cgcaagggat tttcaaacag tggcatacat atgaaaaaaa
    14701 cattactggc agccggtgcg gtactggcgc tctcttcgtc ttttactgtc aacgcagctg
    14761 aaaacgacaa accgcagtat ctttccgact ggtggcacca gagcgttaac gttgtcggaa
    14821 gctatcacac ccgttttgga ccgcagatcc gcaacgatac ctaccttgag tacgaagcat
    14881 tcgctaaaaa agactggttc gacttctatg gttatgcgga tgcgccggta ttcttcggcg
    14941 gtaactccga tgccaaaggt atctggaacc acggctctcc gctgtttatg gaaatcgaac
    15001 cacgtttctc catcgacaag ctgaccaata ctgaccttag cttcggtccg ttcaaagagt
    15061 ggtacttcgc gaacaactac atttacgaca tgggtcgtaa taaagatggt cgccagagca
    15121 cctggtatat gggtctgggt accgacatcg atactggtct gccgatgagc ctgtccatga
    15181 acgtctatgc gaaataccag tggcagaact atggcgcagc gaacgaaaac gagtgggacg
    15241 gttaccgttt caaaattaaa tactttgtgc cgattaccga tctgtggggc ggtcagctga
    15301 gctacatcgg cttcaccaac ttcgactggg gttccgattt aggggatgac agcggtaacg
    15361 caatcaacgg tattaagacc cgtactaata actctatcgc ttccagccat attctggctc
    15421 tgaactacga tcactggcac tactctgtcg tagctcgtta ctggcacgac ggtggtcagt
    15481 ggaacgacga tgcagaactg aacttcggca acggcaactt caacgttcgc tctaccggct
    15541 ggggtggtta cctggttgta ggttacaact tctgattatg aaaatgccgg gatttattcc
    15601 cggcatttca gattgttaac aacgtgcgct ttgttcatgc cggatgcggc gtaaacgctt
    15661 tgtccgaccc acaaaagcac gtaaattcaa tatattgctg aaataacgta gtcctgataa
    15721 gcgtagcgca tcaggcaatt ttacgtttta atcacttctt cttgttcatc atcgctttca
    15781 ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct tcccctgcga
    15841 taacggtcgt accatactga tccgcttcgg tatatttcga atgctcatga tcgtggcaat
    15901 agagacaaag caattcccag ttactgccat cttccgggtt attggtatgg tcgtgatcaa
    15961 tgtggtgaac agtaagttca cgcaggttgg aatagacaaa ctcgcgggaa cagcgaccgc
    16021 agacccacgg atagattttt aatgcttttt cgcgatagcc gctttctaac cgcgcatagt
    16081 tttttgggat gatagccatg aaaagttacc tggatgaaat gtagggttac gcgcagtttc
    16141 ccaagatgcg gggattttcg caatctccat acatcaaaaa aaatcccgat cttctgaccg
    16201 ggattttcaa catcaacggg aacttgatta aggcagaatt gacggctgat ccgccccttc
    16261 tttttccact ttctgctgca acatgtgttc gcgcttcata cccagtttca gagccaacgc
    16321 agatgccaca tagatggaag atgcagtacc gatggaaaca ccgataagca tggtcagcga
    16381 gaagccttcc agtaccggac caccgaagag gtacagcatc aggataacca tcaaggtagt
    16441 accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa agatttcgta
    16501 aggcgtaccg cgacggatct tgcggaagtt ttcacgaata cggtccgata ccacgatact
    16561 gtcgttaagc gagtaaccga taaccgacat caacgatgcc acaatggtca ggtcaatctc
    16621 gatatggaat aacgacaaaa tacccagcgt aatgataacg tcgtgcgcca gcgcaataac
    16681 aacccctgcc gccagtcgcc actcaaagcg aaaacctacg tacacaagga tagacagcag
    16741 cgccgccatc aacgccatcg caccggtttg cgcaaggtct gcccccacgc tcggaccgac
    16801 gaactcaata cgcttcactg ctgcattctg attggtggat tcgttaatca ccttcagaac
    16861 ctggctgccc agcacctgac cgccggtttc gccttcagca ggcggcatac ggaccatgat
    16921 gtcgtggctg ctaccaaagt tttgcagcat tggctcttca aaaccggctt tttgcaatgc
    16981 atcacgcatt acgtcaattt cagccggttt ttcgagcgta atttcgataa ccgtaccacc
    17041 ggtaaaatcc agcccccagt taaagccgcg cacgcccata ataacgatag cagcgattaa
    17101 cagcagacca gagatgccga aagcccagta gtcccagcgc ataaagtcat agactttacg
    17161 gccgtggttt agttgttcaa cagtatattc ctgtgccaca tcgcactcct cagattgaca
    17221 gcttcttgac gcgcttgccg ccatatagca ggtttacgat ggcacgcgta ccgacaatcg
    17281 cggtaaacat cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct ttaattgccc
    17341 cggtacccac tgcgtacagg atgatgactt taatcagcgt ggtgatgttc gcatcgaaga
    17401 tagaactgaa tgcgccacga taaccttcat caattgcctg ttgaacagta cgtccgttgc
    17461 tcaactcttc tttaatacgt tcgttgatca gtacgttcgc atcgaccgcc accgcaaggg
    17521 ttaagacgat acccgcgata cctggcatac tcagcgttgc gcctggcaac agcgacataa
    17581 tgccgacgat taagatcaag ttggcaatca gagcactggt cgcaatcaga ccaaacttct
    17641 tatagaagat gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa gcctccagcc
    17701 cctgttcaat gttctgcata cccagggttg ggccaatggt acgttcttca acaatctgaa
    17761 tcggcgcgat caacgcaccg gcacgcagca gcagtgacag ctgacgggct tcgttcgggt
    17821 tgttgatgcc ggtgatacgg aagctgttac ccagacgaga ctggatgttg gcgatgttaa
    17881 tcacctcttc ctgtttcacc agaaccgcac gaccatttgc atctttctta ccgctgtctt
    17941 tgtactccac aaacagggtt gccatcggtt tgccgatgtt gtccttagtg aagttagaca
    18001 tgatgttacc accagcgcta tcgagcgaga tgttaacctg cggttggttg tattcgtcct
    18061 ggctggaagt ggagtcggtg atatggtcac cggtcagaat tacgcgtttg tacagcacaa
    18121 ctggctgacc ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga ccggatgccg
    18181 cagcggcctg gtcaacgttg gtgtttacca gacggaattc cagcgttgcc gtcgcaccca
    18241 gaatctcttt cgcacgcgca gtgtcctgaa tacctggcag ttcaacaacg atacggtcag
    18301 caccctgacg ctgaaccacc ggctccgcca cgccaagttg gtttacacgg ttacgcagga
    18361 tattgatgtt ctgctgcacc gcatattcac gcgcttcact cagacgagca tcgctcatta
    18421 ccgcacgcag ctggttgctg ccctggctac taatcaccag gtccggatgg cgcttgctca
    18481 gatacgcaat ggcttcatca cgagctttag catcgcggaa agtaatgctc aggccgtagt
    18541 tgttttcttt acgaacggtg gtatacggga tgcccttttc acgcagatcg ctgcgcaggc
    18601 tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc catatccact tccatcagga
    18661 agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg ctcagcgtga atagctgcca
    18721 gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac gtatttgtca cccataacgc
    18781 ccattaatgc ttcacgagcg cgcagctggg tgtcagtgga gtcgaagcgc gcaagaatag
    18841 cgccctcttc cagtgccaca gacttagcag ttattttttc ttcttgtaac gttttctgga
    18901 cctggatcag cgtttgctca ctggcggcga ctccgcgcgc accagtgatc tgaacagccg
    18961 gatcctcacc aaacaggttg ggaagcgcat acagcagacc gatgacaatc accacgatca
    19021 gcatgacgta cttccacaaa ggataacggt ttaacacggc aattccctta gggaaaaatt
    19081 ttaattacag cgccttcatg gtgcctttcg gcaggacggc agctacgaag tcacgtttaa
    19141 taactacttc agtggtgtca ttcagcgcga tagcaatgta gccgttttcc gctactttgg
    19201 ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc acctttggca atggagtcca
    19261 tcagcttttt gtgttctttg gtgcgcttct gctgtggacg caggatcatg aaatagaaaa
    19321 tcagaccgaa taccaccagc atcaaaatca aagacatcgg gctaccttgc gccggtgcac
    19381 ccgttgccgc taccgcatca gaaataaaaa agctcattaa atttccctca ttattaatat
    19441 taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc agttacgaag
    19501 ctctctaatt taccctcttc aatagccttg cgtaaacccg ccatcaaacg ctggtagtaa
    19561 cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg gtcaagatga
    19621 tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat cacactcagg atcgagtggg
    19681 ccagtatcgc tcttatactt cgcattgcgg attttcacca cgccatcggt cacgaacaaa
    19741 tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa acatatcgat accacgacgt
    19801 acgccttcaa ccaggtcttc tggtttacca acgcccatca ggtaacgcgg tttgtctgcc
    19861 ggaatttgcg ggcatacatg ctccagaatg cggtgcatat ctgctttcgg ctcacccaca
    19921 gccagaccgc cgacagcgta gccatcaaaa ccgatatcta ccagaccttt aacagaaata
    19981 tcacgtaaat cttcgtaaac gctgccctga atgataccaa acagcgcatt tttgtttccg
    20041 agactgtcaa aacgctcacg gctacgcttc gcccaacgca gagacatctc catggagcgt
    20101 tttgcgtaat cccagtcagc aggatacggc gtacactcat caaagatcat gacgatatcc
    20161 gaaccaagat cgtactgaat ctccattgat ttttcaggat cgaggaaaat cggatcgccg
    20221 ttgatcgggt tacggaagtg cacgccctgt tcggtgattt tacgaatatc gccaaggctg
    20281 aagacctgga aaccgccgga gtcggtgagg atcggcccct tccactgcat aaaatcgtgc
    20341 agatcgccgt gcagtttcat gatttcctgg cccgggcgca gccacaggtg gaaggtgttg
    20401 ccgaggataa tttgcgcgcc agtggcttca acttcttccg gcgtcatccc ttttacggtg
    20461 ccgtaggtgc caacaggcat aaaacaaggc gtttccacta cgccacgatc aaagaccagg
    20521 cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt ttctccacta
    20581 cgtcagaaaa acagtccaac gttttaaacc agcgccgcgg aattactccc cgacgcgctc
    20641 attaattgcc tgcggattgt acgtgataaa catcgcatca ccgtaactaa aaaagcgata
    20701 tttctcttct accgctgctt tataggcatt catggtgtgt tgataaccgg caaaggccga
    20761 aaccagcata atcagcgtcg attccggcag gtggaagttt gtcaccagcg catcgaccac
    20821 tttgtactgg aagcccggat agataaagat ttgggtatcg tcaaagaacg gttcgataag
    20881 atcgtttttc gccgcctgag ccgcgctttc cagcgaacgt actgaggtgg tgccaaccgc
    20941 aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg tctaccacat cctgcggcac
    21001 ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg tcgacgcgca ccggctggaa
    21061 ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc acgcctttgg cgcgtaattt
    21121 ttccagcaaa ggttcgtcaa aatgcagacc tgcggtcggg gctgcaaccg cgcccggttt
    21181 ttcgctataa acggtttgat aaagttcgcg gtcagcgtct tcgtccggac ggtcgatata
    21241 cggcggcagc ggcatatggc cgatgctgtt gagaatatcc agcaccgagc gttcatcatt
    21301 aaattcgact tcaaacagtg cgccgtggcg cgcggtcatt gttgcgttaa tactttcgtc
    21361 atcgcccagc agcagttctg cgccaggttt tggcgctttc gaggcgcgaa tatgcgcaag
    21421 aatgcgtttg tcgtcgagca tccgttcaac cagcacttca atcttgccgc cgctggcttt
    21481 acgcccaaac aggcgcgccg ggatcacgcg ggtattatta aaaaccagaa gatcgccggg
    21541 gttgagctta tcaagtaaat cggtgaaagt accgtgcgtc agcgcgcccg tcggcccgtc
    21601 cagcgacagt aaacgacagc tactgcgttc aggcatggga tagtgggcaa tcagggattc
    21661 gggcaattca aaggagaaat cggtaacgcg catgacactg actcagacta aaataagagg
    21721 cgggtagtct agtgccgggg cgctctccct gcaaccttta cccccttttg gatagaatat
    21781 aatcgatgaa ttttttagct cacctgcatt tagcccatct cgcggaaagc tcgctttccg
    21841 gcaatttact ggctgatttc gtacgcggaa atcctgaaga aagttttccg cccgacgtcg
    21901 tggctggcat tcatatgcat cgacgtatcg acgtattaac cgacaatctg ccggaagtcc
    21961 gcgaagcacg ggaatggttt cgtagtgaaa ctcgccgcgt tgcgcctatt acgctggatg
    22021 tcatgtggga tcactttctt tcccgccact ggtcgcagct gtcgccggac tttccgctac
    22081 aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat tttgccggac tcaccgccac
    22141 gttttatcaa tctgaacaat tacttgtggt cagagcagtg gctggtgcgc tatcgcgata
    22201 tggatttcat ccagaacgtg ttaaacggca tggcaagccg ccgcccacgt ctggatgccc
    22261 tgcgtgactc ctggtacgat ttagacgctc attatgacgc cctcgaaacc cgcttctggc
    22321 agttttatcc gcggatgatg gcgcaggcgt cacacaaggc gttataaaac gttagttcat
    22381 ccataccgta gccgaaatgg caggcaaagc cagaatcccg tttgtcagtt gcccatggcc
    22441 ttctttgcgt tgccattgcg cgacattaag aaacggtgac gcggggagca ccacttcaca
    22501 ggcctcgcca cggttgattg ccaccagtac acgttgctga ttcagcacgc ggacaaatac
    22561 cactacgtta tcttccgcat acagcacctg acagccgcca cgacgcagcg cctgactttt
    22621 cttacgcagc gcaatcattc gctggtacag cgcgaataac gccgtatcct gcttttccac
    22681 ctgccagggg aacggtttac ggcaaaacgg atcgtttttg ccatccagtc ctacttcatc
    22741 accgtaataa atgcacggta caccaggcca ggtgaacagc cagaccaccg ccagcggcag
    22801 gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca gtatcgtggc tgtcgagctg
    22861 attaaacata cgtaattgtt gttgatgaga aagccctgcg cggtaattat ccatccaggc
    22921 catacaggtt tgggcatcaa tttgctgcgg atcgtaagag atatcggtat tggcaagaaa
    22981 tccccacaac gggaatgtga agccacgata gttcatggcg gcatcttcca catcggcctg
    23041 taaccattgc cgtgcatcgc caaaatgttc gccgacaata tacgcttccg gctgggtttc
    23101 tttcgccgct tcggtgatcc cagcaacgtg ctgcatatta ttgcgcgccc cacccgcctc
    23161 ccccagcata tgcaccacat ccagccgcca gccgtccata ctccacggcg ctttcagcca
    23221 atggcggaca atactgtctt ccccgcgata aatttcattc accagacttt ccgactgata
    23281 atccagcttc ggcaagctgg catagccaag ccagtcgagc gccgtgccat catcactaaa
    23341 cgagtaccag tcgcgccagg gcgattcagg gttgtgacaa gctccccccg tgccacgatt
    23401 gtgcctgtca aaccaggcat gggaatcgcc actgtggtta aacacgccat ccagcaccag
    23461 ccgcattccc agctgctgcg tattgtggcg taagcgcagc aacgccccat caccgccaaa
    23521 ctgcggatcg acatggcgat aatcctcggt atcgtattta tgtacgctgg gagctttaaa
    23581 caccggattg agatacagcg ccgtcacgcc aagctttttc agatacggca gtttttcgct
    23641 tatcccgtcc agatcgccgc catagaacgt tgatccgccc gcctgcgccg tgaccggttc
    23701 atcccagtca cgcaagatga tctcttgtcc ggctgcatga tggtaataga catgatcctg
    23761 ttcagcttca cgaggaagac tacgcgcaaa acgatcaggg aagatctgat aaaaaatctg
    23821 atccgcagcc cattgtgggc cgatatccgg tacatcgacg gcaaactgct ccagtcgtgc
    23881 cggcggcatt cggctgaagc cctgcggtgt aaaccaacgc tggcgatcgt gccacagcag
    23941 tttgaaactg taacgccgcc ggggttgtcc gctggagaga tcaatcgccg cacgccatgc
    24001 ggtgacgcca ggctgctgct gactgcgctg cttatgcatc gatactgaca tttcttcgtt
    24061 atcgtgttct gtacgcagca taatgcgctg cggtgggtct tcgcccgtca gccacagtgt
    24121 aatgagcagt tgatctttgc tttgtttaac aaatgggggc accggcaggt gccatgcatt
    24181 taacatcata attcccctgt gatgaaaatc tgatcacctt gccatagcct aatgcaggat
    24241 cactcatcat ctgaaggata tttgggggag gaggacgggg gcggataact ttcggaatag
    24301 ccggatcagg atttcctcct gaaccggctt acagaacgca aggattactg gttttcagcc
    24361 agctgacgat cgtggcggcg tttaaacatc cagccaatca acagcacaac aatccacgcg
    24421 aaaccgacat acagcgagat acgcgtatcc gggtgataac caatcaaccc gataataaag
    24481 agcaggaaaa tcaaaccgcc gatggtcgtt gctaccccac ccggcacttt aaatttcagc
    24541 gccttaactt cttctggcgg caaacggcga cggaaggcaa tttgcgacag caggatcata
    24601 atccacaccc acaccgtggc gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc
    24661 ggcatgatgt agttcagata caccgcaaac agcagcgcgg tagtcatcac cagcaccgta
    24721 acccacggaa taccgcgacg cgacgttttg ctgaaaattt tcggcgcgct gccctgctct
    24781 gccataccgt ggagcatacg gcctacgcca aatacatcac tgttaattgc cgacagcgaa
    24841 gcagtcagca caacaaagtt aagaatgctg gcggcaaagg taatgcccat atgctggaac
    24901 gtcagcacga acgggctacc ggcagtgcca acctgattcc acgggtagat agacataatg
    24961 acgaacagcg taccgacgta gaacaccaga atacgcatcg gcacggagtt aatcgcacgc
    25021 ggtatcgatt tctcaggatc tttcgcttca ccggcggtaa tcccgataat ttcgatccca
    25081 ccataagcaa acatcaccat ttgcaacgac atcaccatcc caagccagcc gttactgaag
    25141 aagccgccgt tgctccacag attatgaata ccggtcggtt gcccaccgtt gccaatcccc
    25201 cagatgatga tgccgaaacc ggcgacaatc atgatgatga tggtggcgac tttaaagaac
    25261 gagaaccaga attccagctc accgaatacc ttcacgctca tcaggtttac ggcgcaaatg
    25321 atcagcacca cactcagtac ccaaatccag tgcggcaccg tcgggaacca gacacccata
    25381 tagataccaa aagcggtcac atcggcgatg gcgacaataa ggatttcaaa gcagtaggtc
    25441 cagccggtaa tgtaacctgc cagcgggccg aggttttcct gcgcataacg cgagaaagag
    25501 ctggcggccg ggttatgtac cgacatttcc cccagcgcac gcataatgat atacgccgcg
    25561 ataccaccga taatataggc caacaacacg ctcggaccgg ccattttgat ggcgtctgcc
    25621 gaaccgtaaa acagcccggt gccaattgct gaacccagtg ccataaagcg tatgtggcgg
    25681 gtacttagcc cacgctttag cttgttctta ctttccatca attcctaacc catcaacaca
    25741 ataaaaaacc acggggcatc gcagccccgt ggttaaaaca aatgttcagt gatttagtga
    25801 gcgctggagg tcacctgacg acctgccgcg cgatcccaga taatggccag aaccaccatc
    25861 accactgttg gcattaacca cgccagacct tgttcggcca gcggtaaacg ctgcgcccag
    25921 gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc aaaaagcagg
    25981 ctgataaaca tcggcggagc aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa
    26041 cttaatacaa ccagtgcgat acacggcgga taaatagcgg tcagcaccgg tacggagatc
    26101 tggatcagct ggcttaagcc gaggttagaa accaccatcg agaagccgcc gaggataaac
    26161 accagcgtac gataagagag cggtacgtac tgggcaaaga attctgcaca agcacaggtc
    26221 aggccaactg ccgttaccag gcaggcgatg aagattaacg ccgccagcag gaagctaccg
    26281 ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag cagcgccgtt tgcagactga
    26341 tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg ccaggtacag cagagtcaga
    26401 ccaacacccg ccatcaggcc agcccagacg gtataacggg tcagcagacg cgcttcggta
    26461 acgccacgag aacgcgccgc gttaacaata acgataccaa acaccattgc gcccagcgta
    26521 tccatggtca gatagccgtt aacgaagccg ttagaaaacg cagcgttttg ataagcctca
    26581 gtcgccgtgc tgatagaacc cgccggccaa acaatcgcgg caacagacag gatgaccagc
    26641 gcgataattt tcagcggcgc aaggaagttg cccacggtat ccagcagctt gcccggatag
    26701 agcgaaacca gaataacgat agcgaaatag accaggctgt agataaacag cggcagcgcg
    26761 gaatcaccag tcagcggcgc aatccccact tcaaaggaaa cggtagctgt acgcggcgta
    26821 gcgaaaagcg gccccaccgc caggtaacaa accgttgcca gcagcacgcc agcgacttta
    26881 ccgattggcg tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt
    26941 aataccggca ggccaacggc agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg
    27001 cctgcctgca agccgaccat tggagggaaa ataatgttac ctgcgccgac gaacaacgca
    27061 aatgtcataa agcccagagc gatgatatcg cgcgatctta attgatgggt cataaatcat
    27121 actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg gggatgatac
    27181 agcggtatat ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag
    27241 tttttcgatt taccgcgtta atgcagtcag tatgacagca cctgaagcgc aatttaaacg
    27301 cttataacgt tttaaagcaa ggagggatct aaaaaccaga ggtatatagc gataagaaat
    27361 caccaaccag tataaaacac tatttccgtg aaaaagacat ggctaatcat gcgaacaaaa
    27421 aatcaccaac cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa ataagccagc
    27481 atcggctggc ttatggcaat aaaagatgac aaaggcggat taatcgctgt ttttggcaat
    27541 taaacgttcc gggataacaa aactgaaacg tgttcctttt cctactgtac tctcaatatt
    27601 caggcgactt tcgtgatgat tcaccgcatg tttcacgatc gctaacccta atccgctacc
    27661 gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat
    27721 atgctccggt gcaatgcccg gtccgttatc ttcaacgcta aattcggcac cgtgcggcac
    27781 tcgctgccag cgtacggtga tatgcgtgcc ttctggagta tgattcacgg cgttatagac
    27841 caggttcgaa atcgcactgc gtagctgatc ttcgttgcca gacactttga ggccgttatc
    27901 tatctcaaag gtaaatgtct gttttttctg actcagagtc tgagcctcac gctcaacaac
    27961 gcgcagcatc atcggcacat caaccttttc attgagcaaa tgcgtcggtg cggcttctat
    28021 tttcgacagc gtcagcaact gcttcaccag cccttccatc cgctgggtct gctcgcgcat
    28081 ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc tgctcatcca tcatctccag
    28141 gtaaccctgt aacacggtca atggcgtacg taactcatgg ctcacgttgg caaaaaagtt
    28201 acgccgcgcc ccttccagtt gatgcatttg cgtgacatca cgcgccacca tcagcaactg
    28261 tttgtgggta taaggcatga cgcgaatttc cagatgccgc ccggtgttga gcaccagatt
    28321 gagcgggcga gaaaaatcac gcgttttcag atattgcgta aactccgggt aacgcagcag
    28381 gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa ccaagaattt gttgcgccag
    28441 accgttacac cagaaaatac cgccctcttc agtggtcagc accactgcgt cgggcagcga
    28501 ctccgcgccg ctacgaaagc gtttaatcag attgcccagt tcacggcggc gttttttatt
    28561 tcgcagctgc atctggtgta agccgtatag tagcggttcc cagctaccac gccccggtgg
    28621 cggggtcata ctgcgatcca cccacagcca ccatgaaagg cgcaataaat tccagaaatg
    28681 ccagataagc agtcctgtta ccgatgccag caaaaaccag ggcaggtaac caaaaaatgc
    28741 acccaggatg aaagccggga ggcagcaaag tagcagctcc agcaccagcc ttttccacga
    28801 cagccgttcc agcacgtaag atactccagt taagaaatca taagccctgc tctgcgtccg
    28861 atgagcaagg cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca cggtctgcac
    28921 catgcggtca tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt ggacatcgac
    28981 cgtgcggtct tccacataca cgttagttcc ccagacgtgg tttaacagct gctcgcggct
    29041 gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg tcggccccat
    29101 ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc gggtcgagac ttaatccctg
    29161 catctcaatc acctcttcca ccgccattgg cgaaatacgg cgcattaccg ctttgattcg
    29221 cgccaccagc tccttcggcg aaaacggctt ggtgatatag tcatccgcgc cggtttcaag
    29281 gccgcgcacg cgatcttctt cttcccctct ggcggtcaac atcaccactg gaatatcccg
    29341 ggtcatcgac tcgcgcttga ggtgtttgat gaactggata ccggagccgc caggtaacat
    29401 ccagtcaagg agaattaaat ccggccaggg ttcattcagt tgattcacag cactgtcata
    29461 atcttccgct tcgaccggct gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg
    29521 aattggagct tcatcttcta cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat
    29581 aaataggttg cgatcattaa tgcgacgtca ttatgcgtca gatttatgac agatttatga
    29641 aaagctcgtc gcacatatct tcaggttatt gatttccgtg gcgcagaaaa aagcaaatgg
    29701 cacatctgtt tgggtataat cgcgcccatg ctttttcgcc agggaatcgt tatgcgcatc
    29761 cttcacacct cagactggca tctcggccag aacttctaca gtaaaagccg cgaagctgaa
    29821 catcaggctt ttcttgactg gctgctggaa acagcacaaa cccatcaggt ggatgcgatt
    29881 attgttgctg gtgatgtttt cgataccggc tcgccgccca gttacgcccg cacgttatac
    29941 aaccgttttg ttgtcaattt acagcaaact ggctgtcatc tggtggtact ggcaggaaac
    30001 catgactcgg tcgccacgct gaatgaatcg cgcgatatca tggcgttcct caatacgacc
    30061 gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg gacgccaggc
    30121 gcagtgctgt gccccattcc gtttttacgt ccgcgtgaca ttattaccag ccaggcgggg
    30181 cttaacggta ttgaaaaaca gcagcattta ctggcagcga ttaccgatta ttaccaacaa
    30241 caatatgccg atgcctgcaa actgcgcggc gatcagcctc tgcccatcat cgccacggga
    30301 cacttaacga ccgtgggtgc cagtaaaagt gacgccgtgc gtgacattta tattggcacg
    30361 ctggacgcgt ttccagcaca aaactttcca ccagccgact acatcgcgct cgggcatatt
    30421 caccgcgcac agattattgg cggcatggaa catgttcgct attgcggctc tcccattcca
    30481 ctgagttttg atgaatgcgg aaagagtaaa tatgtccatc tggtgacatt ttcaaacggc
    30541 aaattagaga gcgtggaaaa cctgaacgta ccggtaacgc aacccatggc ggtgctgaaa
    30601 ggcgatctgg cgtcgattac cgcacagctg gaacagtggc gcgatgtatc acaggagccg
    30661 cctgtctggc tggatatcga aatcactact gatgagtatc tgcatgatat tcagcgcaaa
    30721 atccaggcat taaccgaatc attgcctgtt gaagtattgc tggtacgccg gagtcgtgaa
    30781 cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa
    30841 gaggtgttca atcgccgtct ggcactggaa gaactggatg aatcgcagca gcaacgtctg
    30901 cagcatcttt tcaccacgac gttgcatacc ctcgccggag aacacgaagc atgaaaattc
    30961 tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga atggaagatt gatttcaccc
    31021 gcgagccgtt cgccagcaac gggctgtttg ctattaccgg cccaacaggt gcggggaaaa
    31081 ccaccctgct ggacgccatt tgtctggcgc tgtatcacga aactccgcgt ctctctaacg
    31141 tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc cgaatgtctg gcggaggtgg
    31201 agtttgaagt gaaaggtgaa gcgtaccgtg cattctggag ccagaatcgg gcgcgtaacc
    31261 aacccgacgg taatttgcag gtgccacgcg tagagctggc gcgctgcgcc gacggcaaaa
    31321 ttctcgccga caaagtgaaa gataagctgg aactgacagc gacgttaacc gggctggatt
    31381 acgggcgctt cacccgttcg atgctgcttt cgcaggggca atttgctgcc ttcctgaatg
    31441 ccaaacccaa agaacgcgcg gaattgctcg aggagttaac cggcactgaa atctacgggc
    31501 aaatctcggc gatggttttt gagcagcaca aatcggcccg cactgagctg gagaagctgc
    31561 aagcgcaggc cagcggcgtc gcgttgctca cgccggaaca agtgcaatcg ctgacagcga
    31621 gtttgcaggt acttactgac gaagaaaaac agttacttac cgcgcagcag caagaacaac
    31681 aatcactaaa ctggttaacg cgtctggacg aattgcagca agaagccagc cgccgtcagc
    31741 aggccttgca acaggcgtta gccgaagaag aaaaagcgca acctcaactg gcggcgctca
    31801 gtctggcaca accggcacga aatcttcgtc cgcactggga acgcatcgca gaacacagcg
    31861 cggcgctggc gcatactcgc cagcagattg aagaagtaaa tactcgctta cagagcacaa
    31921 tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa ttacagcagc
    31981 agcaacaaag cctgaatacc tggttacagg aacacgaccg cttccgtcag tggaacaacg
    32041 aactggcggg ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag catctgcggc
    32101 aatggcagca acagttaacc catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt
    32161 tgacgttaac cgccgatgaa gttgctaccg ccctggcgca acatgctgag caacgcccac
    32221 tgcgtcagca cctggtcgcg ctacatggac agattgttcc ccaacaaaaa cgtctggcgc
    32281 agttacaggt cgctatccag aatgtcacgc aagaacagac gcaacgtaac gccgcactta
    32341 acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg aaaaccattt
    32401 gcgagcagga agcgcgcatc aaaacgctgg aagctcaacg tgcacagtta caggcgggtc
    32461 agccttgtcc actttgtggt tccaccagcc acccggcggt cgaggcgtat caggcgctgg
    32521 agcctggtgt taatcagtct cgattactgg cgctggaaaa cgaagttaaa aagctcggcg
    32581 aagaaggtgc ggcgctgcgc gggcaactgg acgccttaac aaagcagctt cagcgcgatg
    32641 aaaacgaagc gcaaagcctc cgacaagatg agcaagcact tactcaacaa tggcaagccg
    32701 tcacggccag cctcaatatc accttgcagc cacaggacga tattcaaccg tggctggatg
    32761 cacaagatga gcacgaacgc cagctgcggt tactcagcca acgacatgaa ttacaagggc
    32821 agattgccgc gcataatcag caaattatcc agtatcaaca gcaaattgaa caacgccagc
    32881 agcagctctt aacggcattg gcgggttatg cactgacatt gccacaggaa gatgaagaag
    32941 agagctggtt ggcgacacgt cagcaagaag cgcagagctg gcagcaacgc cagaacgaat
    33001 taaccgcgct gcaaaaccgt attcagcagc tgacgccgat tctggaaacg ttgccgcaaa
    33061 gtgatgatct cccgcacagc gaagaaactg tggcgctgga taactggcgg caggttcatg
    33121 aacaatgtct cgcattacac agccagcagc agacgttaca gcaacaggat gttctggcgg
    33181 cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc gctacaggcc agcgtctttg
    33241 acgatcagca ggcgttcctt gcggcgctaa tggatgaaca aacactaacg cagctggaac
    33301 agctcaagca gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc actcagacag
    33361 cagaaacgct ggcacagcat caacaacacc gacctgacgg gttggctctc actgtgacgg
    33421 tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga
    33481 gtcaaggcga gattcgccag cagctgaagc aggatgcaga taaccgtcag caacaacaaa
    33541 ccttaatgca gcaaattgct caaatgacgc agcaggttga ggactgggga tatctgaatt
    33601 cgctaatagg ttccaaagag ggcgataaat tccgcaagtt tgcccagggg ctgacgctgg
    33661 ataatttagt ccatctcgct aatcagcaac ttacccggct gcacgggcgc tatctgttac
    33721 agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga tacctggcag gcagatgcgg
    33781 tacgcgatac ccgtaccctt tccggcggcg aaagtttcct cgttagtctg gcgctggcgc
    33841 tggcgctttc ggatctggtc agccataaaa cacgtattga ctcgctgttc cttgatgaag
    33901 gttttggcac gctggatagc gaaacgctgg ataccgccct tgatgcgctg gatgccctga
    33961 acgccagtgg caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa gagcgtattc
    34021 cggtgcagat caaagtgaaa aagatcaacg gcctgggcta cagcaaactg gaaagtacgt
    34081 ttgcagtgaa ataactattc agcaggataa tgaatacaga ggggcgaatt atctcttggc
    34141 cttgctggtc gttatcctgc aagctatcac tttattggct acggtgattg gtagccgttc
    34201 tggtagttgt gatggtggta tgaaaaaagt cattttatct ttggctctgg gcacgtttgg
    34261 tttggggatg gccgaatttg gcattatggg cgtgctcacg gagctggcgc ataacgtagg
    34321 aatttcgatt cctgctgccg ggcatatgat ctcgtattat gcactggggg tggtggtcgg
    34381 tgcgccaatc atcgcactct tttccagccg ctactcactc aaacatatct tgttgtttct
    34441 ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt acctgatgct
    34501 cgccattggt cggctggtat ccggctttcc gcatggcgca ttttttggcg tcggagcgat
    34561 cgtgttatca aaaattatca aacccggaaa agtcaccgcc gccgtggcgg ggatggtttc
    34621 cgggatgaca gtcgccaatt tgctgggcat tccgctggga acgtatttaa gtcaggaatt
    34681 tagctggcgt tacacctttt tattgatcgc tgtttttaat attgcggtga tggcatcggt
    34741 ctatttttgg gtgccggata ttcgcgacga ggcgaaagga aagctgcgcg aacaatttca
    34801 ctttttgcgc agcccggccc cgtggttaat tttcgccgcc accatgtttg gcaacgcagg
    34861 tgtgtttgcc tggttcagct acgtaaagcc atacatgatg tttatttccg gtttttcgga
    34921 aacggcgatg acctttatta tgatgttagt tgggctaggg atggtgctgg gaaatatgct
    34981 aagtggcagg atttcaggac gttattcacc actgcgcatt gcagcagtga ctgactttat
    35041 aattgtactg gcactgctga tgctcttttt ctgcggcggc atgaaaacaa cgtcgcttat
    35101 ttttgctttt atttgttgcg cgggattatt tgccctttca gcaccgctac aaatattgtt
    35161 actacaaaac gccaaaggcg gagagttatt aggtgccgca ggtgggcaaa tagcgtttaa
    35221 cctcggtagc gccgtcggcg catattgcgg tggtatgatg ctgacgctgg ggctggcata
    35281 taattacgtg gcgctgcctg ccgccctgct ttcgtttgct gcgatgtcgt cgttgctgct
    35341 gtatggtcgc tataagcgcc agcaagcggc ggatactccg gtgctggcga aaccactggg
    35401 gtaggttata gtctcggtgc tcattactta ttgccggatg cggcgtgaac gccttatccg
    35461 ccctacgcgg ttctggcaca ttttgcaggc ctgataagac gcggcaagcg tcgcatcagg
    35521 catgatgcgc caattgccta cgttttttac tcttgtggcc ataaccacgc agcgccgcgt
    35581 acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg tttcacattc gccgccgaag
    35641 acaaattgtt taatcaactg cccaaccgtt tgatataaac ggtctacatt gctcatcccg
    35701 ccccccagga caattacatc cggatcgaga atattcacga catgtgccag cgattttgcc
    35761 agccgcagct cgtagcgacg caatgccagt tccgctaccg gatcgctttc ttcaaccagg
    35821 ctgataattt cactgccttt cagcgcatgt ccgctcaaac gacgataatc tgtggcaaat
    35881 cccgtgcccg aaataaaggt ttcaatacaa ccttgtttac cgcaataaca agggacttcc
    35941 tcgcgataac gcagttcgtc ttcgtccatc cacggtagcg gattgtgtcc ccactcacct
    36001 gccgtgccat tgccgccgat atgcgcccgc ccattgaatg ccacgcccgc gccgcatccc
    36061 gtgccgataa tcacggcaaa tactgtctgc gctcccgctg ccgcgccatc tactgcttct
    36121 gaaaccgcca gacagttggc gtcatttgcc agccgcactt cccgctgcaa cctcgcgctt
    36181 aagtctttat cgaatggctg accgttgagc caggttgaat tggcattctt aaccacaccg
    36241 gtgtaaggcg aaattgagcc aggaatgccc atacctaccg ttccgcgctg ccctgtcgcc
    36301 tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag tctgccggta atcatcacgc
    36361 ggcgtgggca gacgatggcg gtacaactgc tcccctgcat cgcccagtgc aatcacttca
    36421 gttttggtgc cgcctaaatc gatacctata cgcacggtac tctccttatt tttttcaata
    36481 tcaatagcgt agagacggac aaccggattg gcaatgcaag gccgccgaca attcgttatc
    36541 atgcccgcta aatttaacga caaggccgtg gaaattatca tgctgtggtt caaaaattta
    36601 atggtttacc gtcttagccg cgagatttcg ctgcgtgcag aagagatgga aaaacagcta
    36661 gcctcgatgg catttacccc atgcggcagc caggacatgg cgaagatggg ctgggttcct
    36721 ccgatgggat cgcacagcga tgcgttaacg cacgttgcca atggtcaaat tgttatctgc
    36781 gcacgcaaag aagaaaaaat cctcccgtct ccggtgatta aacaggcgct ggaagcgaaa
    36841 atcgccaagc tggaagcgga acaggcgcgt aagctgaaga aaaccgaaaa agattcgctg
    36901 aaagacgaag tgctgcactc tctgctgccg cgagctttca gccgttttag ccagacaatg
    36961 atgtggatcg acacggttaa tggtttgatt atggtggact gcgccagcgc caaaaaagcg
    37021 gaagatacgc tggcattact gcgtaaaagc ctggggtcgt tacctgttgt accattgagc
    37081 atggaaaacc cgattgaact gacgctaacc gaatgggttc gctccggtag cgcagcacag
    37141 ggcttccagt tgcttgatga agccgagctg aaatcgttgc tggaagatgg cggcgtgatc
    37201 cgcgcgaaga aacaagatct gaccagcgaa gagatcacca atcacatcga agccggaaaa
    37261 gtggtgacta aactggcgct cgactggcag cagcgcattc agtttgtgat gtgcgacgat
    37321 ggttcgctca agcgtctgaa gttctgcgac gagctgcgcg atcaaaacga agatatcgac
    37381 cgtgaagatt tcgcccagcg ttttgatgcc gatttcatcc tgatgactgg tgaactggca
    37441 gcgttaattc aaaacctgat tgaaggatta ggtggcgaag cacaacgtta attgctgatt
    37501 ttcctttaat gccggatgcg acgcctgccg cgtcttatcc ggcgtacgaa gccacaccag
    37561 gcatataatt attcgctacg gcgggcaata atttttagcg cagcaatatt atgcgtttta
    37621 tgctgtaact tgctccatgg acgttgtgtc attgtttttc ctcaagccag tgacgcgctt
    37681 cactgcgaat tttccgcatt gccccggttt tcactaaatc ccgtaacagc gaaagatcgg
    37741 gttgttcgac aaataagagt aatgatggat cctcttttaa ccacgtcgtt ttcacatcgg
    37801 cagcgagctg tggattattc atagctagcg accgatgccg agactcggtc aatgtcgtta
    37861 acaacgacgc cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga tcattgcgac
    37921 tttgcagcaa tgcacgccac cattcatcat ctgatttatc ttttatcgta gccagttccg
    37981 caacaatatc tggagatct
//
LOCUS       DAPPNJ010000018        37985 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000018 DAPPNJ010000000
VERSION     DAPPNJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.018"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            94..765
                     /locus_tag="RPM73_000698"
     CDS             94..765
                     /locus_tag="RPM73_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDX6519090.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            775..1971
                     /locus_tag="RPM73_000699"
     CDS             775..1971
                     /locus_tag="RPM73_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDX6519091.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1949..2530
                     /locus_tag="RPM73_000700"
     CDS             1949..2530
                     /locus_tag="RPM73_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDX6519092.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2532..3305
                     /locus_tag="RPM73_000701"
     CDS             2532..3305
                     /locus_tag="RPM73_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDX6519093.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3490..3765
                     /gene="frmR"
                     /locus_tag="RPM73_000702"
     CDS             3490..3765
                     /gene="frmR"
                     /locus_tag="RPM73_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDX6519094.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3800..4909
                     /gene="frmA"
                     /locus_tag="RPM73_000703"
     CDS             3800..4909
                     /gene="frmA"
                     /locus_tag="RPM73_000703"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDX6519095.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5002..5835
                     /gene="fghA"
                     /locus_tag="RPM73_000704"
     CDS             5002..5835
                     /gene="fghA"
                     /locus_tag="RPM73_000704"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDX6519096.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5960..6499)
                     /locus_tag="RPM73_000705"
     CDS             complement(5960..6499)
                     /locus_tag="RPM73_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDX6519097.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6601..7812)
                     /gene="mhpT"
                     /locus_tag="RPM73_000706"
     CDS             complement(6601..7812)
                     /gene="mhpT"
                     /locus_tag="RPM73_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDX6519098.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7988..9001)
                     /gene="dmpG"
                     /locus_tag="RPM73_000707"
     CDS             complement(7988..9001)
                     /gene="dmpG"
                     /locus_tag="RPM73_000707"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDX6519099.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8998..9948)
                     /gene="mhpF"
                     /locus_tag="RPM73_000708"
     CDS             complement(8998..9948)
                     /gene="mhpF"
                     /locus_tag="RPM73_000708"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDX6519100.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9945..10754)
                     /gene="mhpD"
                     /locus_tag="RPM73_000709"
     CDS             complement(9945..10754)
                     /gene="mhpD"
                     /locus_tag="RPM73_000709"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDX6519101.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10764..11630)
                     /gene="mhpC"
                     /locus_tag="RPM73_000710"
     CDS             complement(10764..11630)
                     /gene="mhpC"
                     /locus_tag="RPM73_000710"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDX6519102.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11648..12592)
                     /gene="mhpB"
                     /locus_tag="RPM73_000711"
     CDS             complement(11648..12592)
                     /gene="mhpB"
                     /locus_tag="RPM73_000711"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDX6519103.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFSQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12594..14258)
                     /locus_tag="RPM73_000712"
     CDS             complement(12594..14258)
                     /locus_tag="RPM73_000712"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDX6519104.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14335..15282
                     /gene="mhpR"
                     /locus_tag="RPM73_000713"
     CDS             14335..15282
                     /gene="mhpR"
                     /locus_tag="RPM73_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDX6519105.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15359..16441
                     /gene="lacI"
                     /locus_tag="RPM73_000714"
     CDS             15359..16441
                     /gene="lacI"
                     /locus_tag="RPM73_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDX6519106.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16564..19638
                     /gene="lacZ"
                     /locus_tag="RPM73_000715"
     CDS             16564..19638
                     /gene="lacZ"
                     /locus_tag="RPM73_000715"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDX6519107.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19690..20943
                     /gene="lacY"
                     /locus_tag="RPM73_000716"
     CDS             19690..20943
                     /gene="lacY"
                     /locus_tag="RPM73_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDX6519108.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSIRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21009..21620
                     /gene="lacA"
                     /locus_tag="RPM73_000717"
     CDS             21009..21620
                     /gene="lacA"
                     /locus_tag="RPM73_000717"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDX6519109.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(21723..22904)
                     /gene="cynX"
                     /locus_tag="RPM73_000718"
     CDS             complement(21723..22904)
                     /gene="cynX"
                     /locus_tag="RPM73_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDX6519110.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQVVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22910..23380)
                     /gene="cynS"
                     /locus_tag="RPM73_000719"
     CDS             complement(22910..23380)
                     /gene="cynS"
                     /locus_tag="RPM73_000719"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDX6519111.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23411..24070)
                     /gene="cynT"
                     /locus_tag="RPM73_000720"
     CDS             complement(23411..24070)
                     /gene="cynT"
                     /locus_tag="RPM73_000720"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDX6519112.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24180..25079
                     /gene="cynR"
                     /locus_tag="RPM73_000721"
     CDS             24180..25079
                     /gene="cynR"
                     /locus_tag="RPM73_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDX6519113.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25119..26402)
                     /gene="codA"
                     /locus_tag="RPM73_000722"
     CDS             complement(25119..26402)
                     /gene="codA"
                     /locus_tag="RPM73_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDX6519114.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26392..27651)
                     /gene="codB"
                     /locus_tag="RPM73_000723"
     CDS             complement(26392..27651)
                     /gene="codB"
                     /locus_tag="RPM73_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDX6519115.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27888..29774)
                     /gene="prpE"
                     /locus_tag="RPM73_000724"
     CDS             complement(27888..29774)
                     /gene="prpE"
                     /locus_tag="RPM73_000724"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDX6519116.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29814..31265)
                     /gene="prpD"
                     /locus_tag="RPM73_000725"
     CDS             complement(29814..31265)
                     /gene="prpD"
                     /locus_tag="RPM73_000725"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDX6519117.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31299..32468)
                     /gene="prpC"
                     /locus_tag="RPM73_000726"
     CDS             complement(31299..32468)
                     /gene="prpC"
                     /locus_tag="RPM73_000726"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDX6519118.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(32513..33403)
                     /gene="prpB"
                     /locus_tag="RPM73_000727"
     CDS             complement(32513..33403)
                     /gene="prpB"
                     /locus_tag="RPM73_000727"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDX6519119.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33641..35227
                     /gene="prpR"
                     /locus_tag="RPM73_000728"
     CDS             33641..35227
                     /gene="prpR"
                     /locus_tag="RPM73_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDX6519120.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35325..35600)
                     /locus_tag="RPM73_000729"
     CDS             complement(35325..35600)
                     /locus_tag="RPM73_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6519121.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35786..36418
                     /locus_tag="RPM73_000730"
     CDS             35786..36418
                     /locus_tag="RPM73_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDX6519122.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
     gene            complement(36435..36680)
                     /locus_tag="RPM73_000731"
     CDS             complement(36435..36680)
                     /locus_tag="RPM73_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519123.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37092..37907)
                     /locus_tag="RPM73_000732"
     CDS             complement(37092..37907)
                     /locus_tag="RPM73_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519124.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT         8967 a        10245 c         9827 g         8946 t
ORIGIN      
        1 catttataaa ttaacatctg gctgcgccag agattacgtc tctgagtgca tcgttgtgct
       61 tgtagtataa taaataaaat ggagtgtcat taagtggata aggttttaga ttcagccctc
      121 ctttcttcgg caaataaaag aaagggtatc cttgctattg gcgcacatcc tgacgatata
      181 gaattaggct gtggcgcatc gcttgctcgt cttgcgcaaa aaggaattta tatcgcagca
      241 gtggtgatga ctaccggcaa ctctggtaca gatggaataa tcgatcgcca tgaagaatcg
      301 cgcaacgccc taaagatatt agggtgccac caaactattc atcttaattt tgctgacacc
      361 cgcgctcatt tacagctcaa tgatatgatt tccgccctgg aagacatcat taaaaatcaa
      421 attccttctg atgttgaaat catgcgggta tataccatgc atgatgccga ccgccatcag
      481 gatcatctcg ctgtttatca agcttcaatg gttgcctgcc gcactattcc acaaattctc
      541 ggctacgaaa ccccaagtac ctggctttca tttatgcctc aggtttttga atccgttaaa
      601 gaagaatatt tcacagtcaa acttgctgca ttaaaaaaac ataaaagtca ggaacgacgt
      661 gattatatgc gtcatgatcg cctgcgtgca gttgcacaat ttcgcgggca acaggtcaat
      721 agcgatctgg gtgaaggctt tgtaattcat aaaatgattc tttgagcaaa ttgcatgaaa
      781 acctggatat ttatctgtat gtccatagca atgttgctat ggtttttaag tacgctaaga
      841 cgtaaaccca gtcaaaagaa aggctgtatt gacgccatta tacctgcgta taacgaaggc
      901 ccgtgtctgg cgcagtcact ggataatcta ctgcgtaacc cttatttttg ccgggtaatt
      961 tgcgttaacg acggctccac ggacaatacc gaagcggtca tggcggaagt caaacgcaaa
     1021 tggggcgacc gctttgttgc cgtcacgcaa aaaaatactg gtaaaggtgg tgcgctgatg
     1081 aatggcctca attatgccac ctgcgaccag gtttttttaa gtgatgccga cacctatgtt
     1141 ccgcccgatc aagacggaat gggctatatg ctggcagaaa ttgagcgcgg tgctgatgcc
     1201 gtaggcggca ttccctctac tgcgttgaaa ggcgcggggc tgttaccgca catccgcgcg
     1261 accgtaaagt tgccgatgat tgttatgaag cgcacgctac agcagctcct gggcggcgca
     1321 ccgtttatta tcagcggtgc ctgcgggatg ttccgtactg atgtattgcg taagttcggt
     1381 ttctcggatc gtactaaagt cgaagacctt gatctcacct ggacattagt ggcaaacggc
     1441 taccgtattc ggcaggcgaa tcgctgcatc gtatacccac aggaatgcaa cagtccgcgt
     1501 gaggagtggc gtcgctggcg gcgttggatt gtgggatacg cggtctgtat gcgcctgcat
     1561 aaaagacttt tatttagccg cttcggtatc ttcagtatat ttcctatgct gttggttgtg
     1621 atttatggcg ttgggattta tctcactacc tggtttaatg aattcatcac caccgggccg
     1681 catggtgtgg tgttggcaat gtttccgctt atctggatcg gcgtagtttg tgttattggt
     1741 gcttttagcg cctggtttca tcgttgctgg ttgttggtgc ctttagcgcc gctttccgtt
     1801 gtgtatgtat tattagctta tgccatctgg attatttatg gacttattgc cttttttact
     1861 ggacgcgaac ctcagcgcga caaacccacc cgctattccg cactggtgga agcgtcaacc
     1921 gcttattccc aaccttctgt cacaggaact gaaaaactat ctgaagctta atgttaaaga
     1981 gaaaaatatt cagattgcag accaggtaat tattgatgaa actgcaggtg aagttgttat
     2041 cggcgcgaat acccgtattt gtcatggtgc cgttattcag ggtccggtag tgattggcgc
     2101 aaactgcctg ataggtaatt atgcgtttat tcgtcctggc acaataatca gcaatggtgt
     2161 aaaaattggt tttgccaccg aaattaaaaa tgcggttatt gaagcggaag caacgattgg
     2221 tccgcaatgt tttattgccg actcggtagt tgcaaaccag gcatatttgg gcgcacaagt
     2281 acgtaccagt aatcatcgtc tggatgaaca gcctgtgtct gttcgaactc cagagggaat
     2341 tatcgctacc ggatgcgata aattaggttg ttatatcggg cagcgttcac gccttggcgt
     2401 acaagttata attttgcctg ggcgaattat ttccccgaac acgcaacttg gcccgcgcgt
     2461 gattgtagaa cgtaatttac ctgccggaac ttattcactc cgacaagaac ttatccgtac
     2521 aggagattaa aatgataaaa cggacgttat tagcggcggc catttttagc gcattgcccg
     2581 cttatgccgg gttaacttcc attaccgcgg gctacgattt taccgattat tctggcgatc
     2641 atggcaaccg taatttagcg tatgctgaac tggtggcgaa agttgaaaac gcaacgctgc
     2701 tttttaatct ttcccagggg cgtcgtgatt atgaaactga aaatttcaat gccactcgcg
     2761 gtcagggtgc ggtctggtat aaatggaata actggctgac aacccgaacg ggtattgcct
     2821 ttgcggataa tacgccggtc tttgcccgcc aggattttcg tcaggatatt aacctggccc
     2881 tgttgccaaa aacgcttttc acgaccggtt atcgctacac taaatattac gatgatgtcg
     2941 aagtcgatgc ctggcaaggc ggcgtatcac tctatactgg cccggtgatc accagctacc
     3001 gctataccca ttatgactcc agcgatgcag gtggtagtta tagcaatatg atttccgtgc
     3061 gtctgaatga cccgcgcggc actggttata cgcaactatg gctaagccgc ggaacaggcg
     3121 cttacaccta tgactggacg ccagaaacac gctacggcag catgaagagc gtcagtttgc
     3181 aacgtattca accgctgact gagcaactta atctcgggct gacggcaggt aaagtgtggt
     3241 acgacacccc aaccgatgat tataacggtc tgcaacttgc agcccatctg acctggaaat
     3301 tctgattcct tctgccgccc gctatccggg gcggccttcc ctgccgatta gccccccctt
     3361 tcctctttgt tttccgacca cattcaccgg ataaatttta ttctccagtg ttatatacta
     3421 taggggggta tgcattgaca tatagcatac ccccctatag tatattgcgt gcagatgatg
     3481 aggtgcgaaa tgcccagtac tccggaagag aagaaaaagg tccttactcg agttcgtcgt
     3541 attcgagggc agattgatgc tctggaacgg tcgctggagg gtgatgccga atgccgtgcc
     3601 atactccaac agatcgctgc cgttcggggc gcggctaacg ggctgatggc agaagtgctt
     3661 gaaagccata tccgggaaac gtttgaccga aatgactgct acagccgcga agtcagccaa
     3721 tccgttgacg acactattga actggttcga gcctatctta aatagctgaa tctattacca
     3781 tattgaggaa gagcgagaga tgaaatcacg tgctgccgtt gcatttgctc ccggtaaacc
     3841 gctggaaatc gttgaaattg acgttgcacc accgaaaaaa ggtgaagtgc tgattaaagt
     3901 cacccatacc ggcgtttgcc ataccgacgc atttaccctc tccggggatg acccggaagg
     3961 tgtattcccg gtggttctcg gtcacgaagg ggccggcgtt gtggttgaag tcggtgaagg
     4021 cgtaaccagc gtcaaacctg gcgaccatgt gatcccgctt tacaccgcgg agtgcggcga
     4081 gtgtgagttc tgtcgttctg gcaaaactaa cctctgtgtt gcggttcgcg aaacccaggg
     4141 taaaggcttg atgccagacg gcaccacccg tttttcttac aacgggcagc cgctttatca
     4201 ctacatgggg tgctctacat tcagtgaata caccgtggtc gcggaagtgt ctctggccaa
     4261 aattaatcca gaagcaaacc atgaacacgt ctgtctgctg ggctgtggcg tgaccaccgg
     4321 tattggcgcg gtgcacaaca cagctaaagt ccagccaggt gattctgttg ccgtgtttgg
     4381 tcttggcgcg attggtctgg cagtggttca gggcgcacgt caggcgaaag cgggacggat
     4441 tatcgctatc gataccaacc cgaagaaatt cgatctggcg cgtcgcttcg gtgctaccga
     4501 ctgcattaac ccgaatgact acgacaaacc gattaaagat gtcctgctgg atatcaacaa
     4561 atggggtatc gaccatacct ttgaatgcat cggtaacgtc aacgtgatgc gtgcggcgct
     4621 ggaaagtgcg caccgcggct ggggtcagtc ggtgatcatc ggggtggcag gttctggtca
     4681 ggaaatctcc acccgtccat tccagttggt cactggtcgc gtatggaaag gttccgcgtt
     4741 tggcggcgtg aaaggtcgtt cccagttacc gggtatggtt gaagatgcga tgaaaggtga
     4801 tatcgatctg gaaccgtttg tcacgcatac catgagcctt gatgaaatta atgacgcctt
     4861 cgacctgatg catgaaggca aatccattcg aaccgtaatt cgttactgat ttcccgcagg
     4921 tataccccgt ccacttcaga cggggttctt aatactctcc ctgggcagcc gtccggggga
     4981 ttaaccgtga gataatgact gatggaactc attgaaaaac atgccagctt tggcggctgg
     5041 caaaatgtgt atcggcatta ttcccaatca ctgaaatgtg aaatgaatgt cggcgtctat
     5101 ctcccaccaa aagccgcgaa tgaaaaattg ccggtgctgt actggctttc gggcctgacc
     5161 tgcaacgagc agaatttcat tactaaatcg gggatgcagc gttacgcggc tgagcacaac
     5221 attattgttg ttgcgccgga caccagtccg cgaggcagtc atgtcgcaga tgccgaccgt
     5281 tacgatctcg ggcaaggtgc cgggttttac ctgaacgcaa cgcaagcgcc gtggaatgaa
     5341 cattacaaaa tgtatgacta tatccgcaac gagctgccgg atttagtgat gcaacatttt
     5401 ccggcaacga ccagaaagtc tatctctggt cattctatgg gcgggctggg cgcgctggtg
     5461 ctggcgttac gtaacccaga tgaatatgtc agcgtctcgg cgttttcgcc cattgtctcc
     5521 ccatcgcaag taccgtgggg acagcaagcc tttgccgcat atcttggtga aaataaagat
     5581 gcctggttgg attacgatcc ggtgagtctt atttcacaag gtcaacgcgt tgcggaaatc
     5641 atggttgatc aggggttgag tgatgatttt tacgcggaac agctgcggac tccaaatctt
     5701 gaaaagatct gccaggagat gaatatcaag acgttaatcc gttatcacga gggttacgac
     5761 cacagctatt attttgtctc cagttttatt ggcgagcata ttgcctacca cgccaataaa
     5821 ctgaatatgc gttgataata gtgcacgact gccggatgcg gcgtgaacgc cttatccgcc
     5881 tacatttcgc ccgcaggccg taggcatgat aagacgcgca tagcgtcgca tcaggcatct
     5941 gcgcacgact tatctatgtt taccacatca aatcatcagg cactttgaag tcggcatacg
     6001 gatcgtcttc atcctgctct tcggcactga gcgcgctgtg caacactata ctgctggcat
     6061 cacgctgagc tattttatcg gcaacgctgg cgggaataat ggcgtattca ccttcgctat
     6121 tgttatcgac caacaagcga gcaatcgcca gacgaccatt aatcaactgc gcttgtgtga
     6181 gcttatcgac aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc
     6241 cattggcaat ggtgattcgg ttcatttcaa tgagctgctt cacctgagct ttatattctt
     6301 tcgccaacgc cgcttgtttt tgctgttcgc taagctgttt atcacgctca agctgtgcct
     6361 ttttattttc ttctaccgcc gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg
     6421 ttctctcgac cttcgccgct tttttgctgg tgactaatcc tgctttgagc aactgctctt
     6481 gtaaggtaag ttttgccatc tttgtttcca aacccgctga ataattttta gcgagtatac
     6541 ctgtaaacgc cgtgggtgta ttgggggcaa gtccctttcg cccctgctta ataaggcgcg
     6601 tcaggcatcg gcacacggct gcattcttga tctccgactc atcaaaataa acaccgccaa
     6661 cccagcaaca agaatacccg gtgcagaagc ggccattacg ccgaccgtgc cagtgcctaa
     6721 tgccagcatt ttcccggcca gtaacggacc gctcatagcc cccagacgcc ctaccgccac
     6781 ggctgttccc acacctgttg cgcggatctg cgaactgtaa aacaacggtg ccagggcata
     6841 caaaacgctt tgtccacctg tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc
     6901 gttaaatgac gacaccgttc caagcgccag cagcgaagct aacatgccgc tataaatcag
     6961 tagcgacatg gttactggac gcagcttatc catcaatgcg cccaacatta acgtcccgct
     7021 tgccgccccc atttgcaggg caaacatcac ccctgccgcc tgcgatggct ggaatccttg
     7081 ctccaccaaa agtagcggta gccagttgat caacatataa accaccagca gagtgaagaa
     7141 ataacacaac cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag
     7201 tggtggcgca gcctgttttt cgccagcgaa aaccgctgac tccggcagcc agcgcattaa
     7261 tagcggcacc agaatcaacg gcaccacacc acctacccaa aacaccgttt gccatgctaa
     7321 gtttgccccc gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata
     7381 catcaggctc actgctgtcc cacgaaaacg tggacccgcg gcttcagacg tcagggcgat
     7441 aagattcggc aacgccgccc ccagcccgac accggtcatc agccgcgcaa agaccagtga
     7501 ggggaaatcc caggcaatcg ccgttgccag cgagaacaaa ccaaacagcg caactgagcc
     7561 aatcaaaatg cgcttgcgac cataacggtc cgccagcatt ccgccaacca acgcgccggg
     7621 tagcaaaccg agtattccgg cgctaaatat ccagcccatt tgcattttat cgagtgcgaa
     7681 agcctgggcg atgccacccg ccgcaatgcc agccgcctga agatccagcc cttccatcag
     7741 agcgaccaaa aaacaaagcc cgatagtcag catcaggcgg gatgaagatg atgaaggggt
     7801 acgagtcgac ataagatgaa cctcgttgcg agtgcaggtg aatcgcgact gatacattgc
     7861 cagccgcgta atcagaacta tttaatgact gcctacgggc gaaatgtagg ccggataagg
     7921 cgttcacgcc gcatccggct gtcgtgctgg gatgggcata caaaatgcac gttatcggca
     7981 gtcatcatta tttgttgttg cgcagatcca gcgccacgtc gacgatcata tcctcctggc
     8041 cgccaaccat ccggcgtttg cccagctcaa cgagaatatc caccgcactt aagccataac
     8101 gcgccgccgc cgtttcacag tgacgcagga agctcgagta aacaccagcg tatcccagcg
     8161 ccagcgtttc gcgatcgact cgtaccggtc gatcctgcaa cggacgcacc aggtcgtcgg
     8221 cggcatccat taacgcatag agatcggtcc catgctgcca gcccagttta tccgcggcgg
     8281 caataaacac ttccagcggt gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt
     8341 cgattcggtc gcagccctct tccaccgccg cgatagagtt cgccacgcca agactcaggt
     8401 tatggtgagc gtgcatgcca gtttgcgttt ctggtttcag ctctgctttc agggcgcgga
     8461 aacggtcacg gatatcgctc atgttcatcg caccgccaga atccaccaca taaatacagg
     8521 tcgcaccgta gccttccatc agctttgcct gctttgcgag attctccggc gtggtcatat
     8581 ggctcatcat cagaaaacca acggtgtcca ttccgagctc gcgggcatac tgaatatgct
     8641 gggcggaaac atcagcttcg gtacagtgcg ttgccacacg aaccacccgc gcgccagcct
     8701 gccaggcatt tttcagatcg tgaatagtgc cgattcctgg cagcagcaac gtcgcgattt
     8761 tggcgtgctt caccacatcc gccgctgctt caatccattc aaggtcgcta tgtgcgccga
     8821 aaccatagtt aaagctggaa ccctgcaaac cgtcgccatg ggccacttca atcgaatcca
     8881 cgcgggcatc gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac
     8941 gaatggcgtg cataccgtca cgcaacgtga cgtccgagat ataaagtttt ttaccgttca
     9001 tgccgcttct cctgccttgc gcgccagtga ctgggccatt ttttccgctg tcgccagcgc
     9061 actggaagtc ataatgtcga ggttgcccgc ataggcaggc agataatgcg ctgcgccttc
     9121 gacttccagc cagaccgccg ttttcagccc ggagaactga cccacgcccg gcaaattaac
     9181 cggtttatcc tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat atcccggtac
     9241 gtaagcctgc accgcctcag ccatttcatt gattgaggct tcgatatcat cttgtgaagc
     9301 ttcgtcgctc aatacataca ccgtgtcacg catcatcaac ggtggctctg ctgggttaag
     9361 cacaataatc gccttccctt ttgccgcgcc gcccaccact tcaatggctc gggaagtggt
     9421 ttccgtaaat tcatcgatat tggcacgcgt gccaggtccg gcagatttac tggcgataga
     9481 agcgataatt tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca ccattggaat
     9541 ggtggcctgg ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag
     9601 gttaaccacc ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat
     9661 atccggtttc gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc
     9721 atcaaataca atgtcgatat cagcaaattc aggcatgttc atcagcccga tcaccccttc
     9781 atgggtggtg gcgacgccca tacgtctggc acgcgccaga ccgtcggact gaggatcaat
     9841 gccgaccatc accgccatct ccagatgctg accgtgacgc aaaattttaa tcatcagatc
     9901 ggtgccaatg ttgccagaac cgataatggc gactttacgc ttactcatga cagacttcct
     9961 tttggggctg cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat atgggcttca
    10021 aaacgatcgc ccgcattcac cgccaccatc gggcctaatg ccccggtaag aatgatatct
    10081 ccggcgcgca gcggttcacc cagactggcc attttgcgtg ccagccagac ggcggcatta
    10141 agcggatgtc ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc
    10201 atcttcatgg cgcagttttt caggtctaac cccgccggac gttgcgccgg accgccgatg
    10261 acatacaccc cgcaggaagc gttatctgcc acggtatcga caaactgaat cgaccagtcg
    10321 cgaatgcggc tccccaccac ttccagcgcc ggaagtaccc attcaatggc gttatacagt
    10381 tcgtcgaagg tgatatcagt tgcgggtaaa tcgcggttca acaccagtgc gatctccgct
    10441 tcaatgcggg gttgcagaac acgggaaaaa ggaatgattt cgttatcgcc ataacacatg
    10501 tcggcaaata acgtcccaaa atccggttga tcaacgccca gttgttgttg cactttcgga
    10561 tgcgtcaggc ccactttacg ccctaccacg cgacgcccct gcacaacgtc atattgcaca
    10621 tttatgtgct gaatggcgta ggccgcttca gcgttatcga taccaatcag atcgcgcagc
    10681 ggcgcaatcg cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga
    10741 gtatgcttcg tcatgaccat tccttaaggg cgtgcgagga aattcagcac caattgattg
    10801 aaagcgtcgg catgttccca ctgcgcccag tgaccacagt cgcggaagat atgcagttca
    10861 gaaccggcaa tgccggacag cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg
    10921 cgcccccaga caatcagggt ttgcgctttg atttccgcca gacgtgggcc aaaatccggg
    10981 aactgtttcg gattagcttc caggctctta acgaagtttt ccaggtgatc gcggcgcgac
    11041 agcatattat tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt atcaaaaacg
    11101 aagatatcca tcatcagctt caggttttcg atagtcggct gacgataaag ctgattcagt
    11161 cgcttaatac cttcggttgg catcggcgta aacaaactca tgccgcccgt accgccgccc
    11221 atcagcacca gtttgccgac gcgctccggc catttaaggg tgaacgccac agaactatgg
    11281 ccccccatcg agttgcccag caggtggatt ttggcgatat ccagttgatc caccacgctt
    11341 ttcaggattc gtgcattaag atccgatcgt gaaccactat taacgatcga atcgcttttg
    11401 ccccaacccg gacaatccag caggattacc cgatagcccg cctctaccag cggatcgata
    11461 ttgcggctga agttcgccca gccagtagca cccgggccgg aaccatgcag caggacaacg
    11521 gtttcgtcgc cttgtccgca gtcattaaaa tggatgcgca gcgttttacc cgcttcttct
    11581 acattcagaa aacggctggt ggcggcttcg gtttgtggct gataactcat catcttctcc
    11641 tgcatattca gttctctgtt ctggcgctta acgagccaaa tccggcaatc cactccggga
    11701 ttgggcggta ataacgccct tcgctacgcc agttgccaaa tgtagaaata gcggcaaaag
    11761 cggcgaccca ggttttgatt tcatgtgtcg actttccggc aatggcggaa agctcttcgt
    11821 tactgacggc atccagttcc tgtatgcgtc cctgctccag caaagtcatg aactggttat
    11881 cccaaatcgg gttgagcgga tgcagagttc tctgatcctc aacaaacttc tcagcggcgc
    11941 taatcacccg ttgctgacgc aattcgcgct cactggcggg taaatctttc ccgctcccca
    12001 acagacggtc gcgcatatgg gcatcggctt tcgccagttc gggcaccggc ggctgatggg
    12061 aaagcccacc ggaacccagg aacagcacgc gtttattgag agtgctggtg aaacgtccaa
    12121 tggcttcacc caacatgcgg gtacgctgga aaccgggcag cggcgtggcg acaccgttga
    12181 tgaacacagg cagaactggc accttatcca gcccaccgag caggaactcc agcggctggg
    12241 agaacccgtg gtccacctgc atacagtaag aaacggcaag atcgatcccg ctcttcatga
    12301 cggcatgcgc acaggcctcc gccagctcca caggcacggg cagctctcct gccgcactgc
    12361 cgaaatcacc aattgccgtc gctccaacgc ctaaacagaa cggtggcatc acgtcataga
    12421 aaaagccgtt gtagtgatct ggcgcaaaca gcaccaccag ttcaggggag aaagccgcaa
    12481 tacgctcgcg ggcgctggca atcacgccat tgacctcatc gagtacctct tgtgccgggt
    12541 cgacataccc caccagcggc gagtgggaaa gacaatgaag ataagcgtgc atatcaggct
    12601 accttttcga cagaaacgtc ggcatcaggg cgggtcagcg tcatcaccga cgccagttta
    12661 ttcagggtct tgcccagggt ttgcggaatg gcggtggcgg caacaaagcg atccgggcgc
    12721 atcaccacca gcgaagcatt gtgttgtgcg aaccagctac gcagacgacc ttgcgtatcg
    12781 cccacgcgta gcacgccgtc gtggttatcc tgtgccgtat gaatttgcac ttccggcacc
    12841 acctgaataa agcgtgtgcc caacgcgcgc cactgctgga tttgctcgtc gctcatcccc
    12901 cacagtggat tgcatcccca gccaattacc gcgaagttcg cgccgatcgc gttatcgagc
    12961 agcgtcacgt cgccgttttc cagcgtgact ttcggctgaa taaacatctt gccgaccgga
    13021 gagtgcttcg cttcgccctc acgcatcagc gcaccgccgt aatattgcgg catcggcttg
    13081 aagcgcattt cgaggaagta gcgttttact ggcggcagat aattcagtag ccaggaaacg
    13141 ccgtcacgta acgtaccctg ccagcgtttc ggcggagcca gcacgttgcc cgccgtcacg
    13201 gacaggtcaa tcatcgcttt ggcgtgatcg cgacgttctt gttgataggt atcgagcagc
    13261 gcatcgcggg ctttcccctg gataaccaac gccagtttcc aggcgaggtt aaaggcgtcg
    13321 cgcataccac tgttatagcc ctgcccctgc cataccggca tgatgtgcgc ggcatcgccc
    13381 gccagcagta cgcgatcaat acggaaacgt tgcgccagtc gcgcgttgtg ggtgtagaca
    13441 cgctggcgaa tcaattcaac attgtccgga ttaggcagca ctttgcttaa cagcttgcgc
    13501 atattttgcg gctcacgcag ctgttcttcg gtttctcccg gcatcaccat aaattcaaag
    13561 cgacgtaccg catgaggcag cgcggcagaa acatacgggc gcaccggatc gcaacataaa
    13621 tagatatgcg gcgtacttaa cggatcgttg gcgatatcca ccacaatcca ctgatttggc
    13681 gcagttttac cttcaaacgg cacattcagg gtgcgacgaa caaaacttgc cccaccatca
    13741 caggccacca gccactgggc tttgactatt tcccgctgcc cttctgccgt tttcaggtgc
    13801 aaggtcactt cgtcattttg ctggctgaag gcctccagct cgcgggcaaa caagcagcgc
    13861 acattcggaa aacgcgacaa cccttccagc atcaccgcat cgacctgtgg ctgaataaag
    13921 gcgttacggc gcggccagcc aaattcatcg gtcattggct gaatatcagc aaaacagcgg
    13981 ccttttgggg tgagaaaacg catcgcgtgc cacggtgtag tgtgcggcag aacattctcg
    14041 accaggccga ccgactgcat ggtgcgcagc gcctcgtcat caataccaat cgcacgcggg
    14101 tagtcgatca acttatcgag tttctccacc accagcacgt caatgcccat ctgaccgaga
    14161 tagttcgcca tcatcagccc aaccggaccg gcaccagcga tcgccacctg aacgctatgg
    14221 ttaacagcag gctggatgtc agggtgttgt attgccattt cagtacctca cgactcggac
    14281 aaaatgccgt tgcgcgcaca gtacagcgca acttattttg ttaaaaacat gtaaatgatt
    14341 ttttattgtg cgctcagtat aggaagggta ttttcggcta caatcaaaac atgcccgaat
    14401 gtgcaccagg tgcacaacgt tgttttaact atagaaatgt caattaatat gcagaacaat
    14461 gagcagacgg aatacaaaac cgtgcgcggc ttaacccgcg gtctaatgtt attaaatatg
    14521 ttaaataaac ttgatggcgg tgccagcgtc gggctgctgg cggaactcag cggcctgcat
    14581 cgcaccactg tgcggcgact gctggagacg ctgcaggaag agggatatgt ccgccgtagc
    14641 ccctccgatg acagttttcg tctgaccatc aaagtgcggc aattaagcga aggatttcgt
    14701 gacgaacagt ggatttctgc actggcggcc ccgctgctgg gcgatctgtt gcgcgaagtg
    14761 gtatggccaa cagatgtgtc caccctggat gttgatgcaa tggtggtacg cgaaaccacc
    14821 caccgtttca gccgcttatc ctttcaccgg gcaatggtcg ggcgacgttt accgctcctg
    14881 aaaaccgcct cgggcctgac ctggctggcc ttttgcccgg aacaagaacg caaggaatta
    14941 atcgaaatgt tagccgcccg ccccggtgat gactatcaac tggcacgaga accgttaaag
    15001 ctgcaagcca ttctggcgcg cgcgcgcaaa gagggttacg gacagaacta ccgcggctgg
    15061 gatcaggagg agaagatcgc ctctatcgcc gtaccgctgc gcagtgaaca acgggtgatt
    15121 ggctgtctga atctggtgta tatggcgagc gcaatgacca ttgaacaggc cgcggaaaag
    15181 catcttccgg cgctacaacg ggtagcaaaa cagatcgaag aaggggttga atcgcaggct
    15241 attctggtag ccggacggcg aagcggcgtg catttacgtt gacaccatcg aatggcgcaa
    15301 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt
    15361 gaaaccagta acgctatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
    15421 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
    15481 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
    15541 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
    15601 ggcgattaaa tctcgcgctg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
    15661 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
    15721 gctgctcatt aactatccgc tggatgacca ggatgccact gctgtggaag cagcctgcgc
    15781 taatgttccg gcgttatttc ttgatgtctc tgaccagact cccatcaaca gtattatttt
    15841 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
    15901 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
    15961 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
    16021 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
    16081 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
    16141 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
    16201 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
    16261 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
    16321 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
    16381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    16441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    16501 tgcttccggc tcgtatgttg tgtgaaattg tgagcggata acaatttcac acaggaaaca
    16561 gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    16621 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    16681 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    16741 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
    16801 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
    16861 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
    16921 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
    16981 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cggtcgctgg
    17041 gtcggttacg gtcaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
    17101 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
    17161 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
    17221 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
    17281 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
    17341 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
    17401 atcgatgagc gtggtagtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
    17461 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
    17521 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
    17581 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
    17641 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
    17701 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
    17761 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
    17821 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
    17881 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
    17941 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
    18001 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccttcgc aaccgatatt
    18061 atttgcccga tgtacgcgcg cgtggatgaa gaccaaccct tcccggcggt gccgaaatgg
    18121 tccatcaaaa aatggctttc gctgcctgga gaactgcgcc cactgatcct ttgcgaatat
    18181 gcccacgcaa tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
    18241 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
    18301 gatgaaaacg gcaacccgtg gttggcttac ggcggtgatt ttggcgatac gccgaacgat
    18361 cgccagttct gcatgaacgg tctggtcttt gccgaccgca cgccgcatcc ggcgctgacg
    18421 gaagcaaaat accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
    18481 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
    18541 gatggcaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
    18601 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
    18661 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
    18721 cagtggcgtc tggcggaaaa cctcagtgtg acgctcccct ccgcgtccca tatcatcccg
    18781 caactaacca ccagcgaaac ggatttttgc atcgagctgg gtaataagcg ttggcaattt
    18841 aaccgtcagt caggccttct ttcacagatg tggattggcg atgaaaaaca actgctgacg
    18901 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
    18961 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
    19021 gaagcagcgt tgttgcagtg ctcggcagat acacttgccg acgcggtgct gattacgacc
    19081 gctcacgcgt ggcagcatca gggaaaaacc ttatttatca gccggaaaac ctaccggatt
    19141 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tagcgagcga tacaccgcat
    19201 ccggcacgga ttggcctgac ctgccagctg gcgcaggtcg cagagcgggt aaactggctc
    19261 ggattagggc cgcaagaaaa ctatcccgac cgccttactg cggcctgttt tgaccgctgg
    19321 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
    19381 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
    19441 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
    19501 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
    19561 tggagcccgt cagtgtcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg
    19621 gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg
    19681 aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt attctttttc
    19741 ttttactttt ttatcatggg agcctacttc ccgtttttcc cgatttggct acatgacatc
    19801 aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct gttctcgcta
    19861 ttattccaac cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa atacctgctg
    19921 tggattatta ccggcatgtt agtgatgttt gcgccgttct ttatttttat cttcgggcca
    19981 ctgttacaat acaacatttt agtaggatcg attgttggtg gtatttatct aggcttttgt
    20041 tttaacgccg gtgcgccagc agtagaggca tttattgaga aagtcagccg tcgcagtaat
    20101 ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct gggcgctgtg tgcctcgatt
    20161 gtcggcatca tgttcaccgt caataatcag tttgttttct ggctgggttc tggctgtgca
    20221 ctcatcctcg ccgttttact ctttttcgcc aaaacggatg cgccctcttc cgccacggtt
    20281 gccaatgcgg taggtgccaa ccattcggca tttagcctta agctggcgct ggaactgttc
    20341 agacagccaa aactgtggtt tttgtcactg tatgttattg gcgtttcctg cacctacgat
    20401 gtttttgacc aacagtttgc taatttcttt acttcgttct ttgctaccgg tgaacagggt
    20461 acgcgggtat ttggctacgt aacgacaatg ggcgaattac ttaacgcctc gattatgttc
    20521 tttgcgccac tgattattaa tcgcatcggt gggaaaaatg ccctgctgct ggctggcact
    20581 attatgtcta tacgtattat tggctcatcg ttcgccacct cagcgctgga agtggttatt
    20641 ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg tgggctgctt taaatatatt
    20701 accagccagt ttgaagtgcg tttttcagcg acgatttatc tggtctgttt ctgcttcttt
    20761 aagcaactgg cgatgatttt tatgtctgta ctggcgggca atatgtatga aagcatcggt
    20821 ttccagggcg cttatctggt gctgggtctg gtggcgctgg gcttcacctt aatttccgtg
    20881 ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc gtcaggtgaa tgaagtcgct
    20941 taagcaatca atgtcggatg cggcgcgagc gccttatccg accaacatat cataacggag
    21001 tgatcgcatt gaacatgcca atgaccgaaa gaataagagc aggcaagcta tttaccgata
    21061 tgtgcgaagg cttaccggaa aaaagacttc gtgggaaaac gttaatgtat gagtttaatc
    21121 actcgcatcc atcagaagtt gaaaaaagag aaagcctgat taaagaaatg tttgccacgg
    21181 taggggaaaa cgcctgggta gaaccgcctg tctatttctc ttacggttcc aacatccata
    21241 taggccgcaa tttttatgca aatttcaatt taaccattgt cgatgactac acggtaacaa
    21301 tcggtgataa cgtactgatt gcacccaacg ttactctttc cgttacggga caccctgtac
    21361 accatgaatt gagaaaaaac ggcgagatgt actcttttcc gataacgatt ggcaataacg
    21421 tctggatcgg aagtcatgtg gttattaatc caggcgtcac catcggggat aattctgtta
    21481 ttggcgcggg tagtatcgtc acaaaagaca ttccaccaaa cgtcgtggcg gctggcgttc
    21541 cttgtcgggt tattcgcgaa ataaacgacc gggataagca ctattatttc aaagattata
    21601 aagttgaatc gtcagtttaa attataaaaa ttgcctgata cgctgcgctt atcaggccta
    21661 caagttcagc gatctacatt agccgcatcc ggcatgaaca aagcgcagga acaagcgtcg
    21721 catcatgcct ctttgaccca cagctgcgga aaacgtgctg gtgcaaaacg cagggttatg
    21781 atcatcagcc caacgacgca cagcgcatga aacgcccagt ccatcaggta attgccgctg
    21841 atactacgca gcacgccaga aaaccacggg gcaagcccgg cgatgataaa accgatcccc
    21901 tgcataaacg ccaccagctt gccagcaata gccggttgcg cagagtgatc gagcgccagc
    21961 agcaaacaga gcggaaacgc gccgcccaga cctaacccac acaccatcgc ccacaatacc
    22021 ggcaattgca tcggcagcca gataaagccg cagaacccca ccagttgtaa caccagcgcc
    22081 agcattaaca gtttgcgccg atcctgatgg cgagccatag caggcatcag caaagctcct
    22141 gcggcttgcc caagcgtcat caatgccagc aaggaaccgc tgtactgcgc gctggcacca
    22201 atctcaatat agaaggcggg taaccaggca atcaggctgg cgtaaccacc gttaatcaga
    22261 ccaaagtaaa cacccagcgt ccacgcgcga ggagtgaaaa ccacgcgaac cggagtggtt
    22321 gttgtcttgt gggaagaggc gacctcgcgg gtgctttgcc accaccaggc aaagagcgca
    22381 acaacggcag gcagcgccca ccaggcgagt gtttgatacc aggtttcgct atgttgaact
    22441 aaccagggcg ttatggcggc accaagtcca ccgccgccca tcagagccgc ggaccacagc
    22501 cccatcacca gtggcgtgcg ctgctgaaac cgccgtttaa tcactgaagg catcaccacc
    22561 tgaatgatac cgatccccac cccaccaagc agtgcgctgc taagcagcag cacactttgg
    22621 gggtaaagct cacgcatcaa tgcaccgacg gcaatcagca acagactgat ggcgacactg
    22681 cgacgttcgc tgacatgctg atgaagccag cttccggcca gcgccagccc gcccatggtg
    22741 accaccggaa gagcggtcaa cagggcagcc acgctaaagc tcattccgct cgcctggcgc
    22801 aattgcggta gcagtggccc gacggaggtg agcagtggtc gcatattaag accaatcagc
    22861 accagaacca gcagcatcgt gaacgtcttt tgatgattgc gcatggctgt cagaacggct
    22921 tggtcggcag atatttacca tctaaggtaa tgaccgcacg ttcgccacct tccgggtccg
    22981 ccactttctt aacgtcgagt ttgaagttaa tcgcgctgat aatgccatcg ccaaacttct
    23041 catgaaccaa cgctttcagg gttgtgccgt acacctgcaa catttcatag aaacgataca
    23101 tcgttgggtc ggttggaata cggtcatcaa tgcagccacg cagtggaatc atctgcaaca
    23161 gtagaatggc gtcttcgtcg agatccagct tcgccccgac caggcgggcg gcgtcggcag
    23221 gaagcgcttg ctgccccagc aaagccgcgg ttacgaaggc ttctgccaga ccggtaccgt
    23281 cggcaatctc ggcaaatgag agatcttttt tagctttgct gagcaaaatg gcatcggcaa
    23341 gatcaagacg aatattgcgg ttaatttgtg actgaatcat ggtggatctc ctgatggttt
    23401 aaaaataagg ttacgctgcg gtcggttggc gtagcggtat ggcacaaacg cgaggattag
    23461 cggccagtgg cacaaactgg cgggttgcgc cgtcaaaagc tgcgatgctg ccgctttcaa
    23521 tgtcgtagac ccagccgtgc agggcgatcc gcccctcttc gagcgccagg cgcaccgatg
    23581 gatgagtttg caaattagcc aactgagcaa tgacgttttc acgtaccatc gccgcagctt
    23641 ttgacggtaa atcggaatgc gggcgcgcct cattaacgac gcgggctgaa tcggcataac
    23701 gtagccagtg ggagacggca ggcatatggt ccatgcactg acagctggca atggcggtca
    23761 tcgcgccaca gttggaatga ccacaaatca caatgtcaga tacccgaagc gcagcgacgg
    23821 catactccac cgaagcagaa acgccaccgg gttccggccc gtaggaaggg acgatattgc
    23881 ccgcgttgcg aataacgaac agatcgccag gctcacgttg cgtcaccagc tcagggacca
    23941 gacggctgtc ggagcaggag ataaaaagtg tgcgcgggct ttgctgtgtc gccagctgtt
    24001 taaacaaggc ttcccgcttc ggaaatgcct cgcgctggaa tttaaggaat ccatcaataa
    24061 tctctttcac cgttaacctc tgtctgtctc tggaatgaga ggcagactac tcgccgattg
    24121 tcataaggta aaagtctcat ttatgatgag ttctattgga tttacttata ggttggcgaa
    24181 tgctctctcg acatatcaat tattttcttg ccgtggctga acatggcagc ttcacccgtg
    24241 ccgccagtgc gttgcacgtc tctcagcctg cgctttccca gcagattcgc cagttagagg
    24301 agagtttagg cgtgccgctg tttgaccgta gcgggcgaac gattcgtctc accgatgcag
    24361 gagaagtctg gcgacagtac gccagccggg cgttacagga actgggggcg ggtaaacggg
    24421 cgattcatga tgttgccgat ctgacgcgag gatcgctgcg tatcgccgtc actcccacct
    24481 ttacaagcta ctttatcggc cctttaatgg cggatttcta tgcgcgctac cccggcatca
    24541 cgctccagct acaggaaatg tcgcaggaga aaatcgaaga tctgctttgt cgcgacgagc
    24601 tggacgttgg gattgccttc gcgcctgtgc attcgccgga gctggaggca attcctttac
    24661 tgacagaaag tttagcgttg gtggtggcga aacatcatcc gctggctgcc tgtgaacagg
    24721 tggcgttgag tcgcttgcat gatgaaaaac tggtcctgct cagcgcggaa tttgccacca
    24781 gagagcaaat tgaccactac tgcgagaaag cggggctaca tccacaggtg gtcattgagg
    24841 cgaactcaat tagcgcggtt ctggagctga ttcgccgcac ttccctttcc acattgttac
    24901 cagcagcgat tgccacacaa catgacgggc ttaaagcgat ttctcttgcc ccgccactac
    24961 tggagagaac ggcggttttg ttgcggcgga aaaatagctg gcagacagcc gccgcgaagg
    25021 catttttgca catggcgttg gaggaatgtg cggatgttgg cgagaatgaa tcacggtagc
    25081 cactgacaca ggtcagtggc tcttcaacac agaggaaatt accgtttgta atcgatggct
    25141 tctggctgct ccagatatac ggtggtttgt gccggttgtg tactggcaat caccttgccg
    25201 ccacgtaccg aataacgtac cggaacctga cggcgcagcg catcaaatcc attttcagcc
    25261 ggcaggataa tcaggtttgc gctgtttccg gcggcaatgc cgtaatcctg caaattcaat
    25321 gttctggcac tgtggtgggt gattaaattc aggccatcgt taatctgccc gtagcccatc
    25381 aactggcaaa catgcagccc catatgcagc acttgcagca tattcgccgt tcccagcgga
    25441 taccacggat cgaagacatc atcgtgacca aagcagacgt taatgccgga ctccagcatc
    25501 tctttaacgc gcgtgatgcc gcgacgtttt ggatacgtat cgaaacgtcc ttgcagatga
    25561 atattgacca gcgggttggc gacaaagtta ataccggaca ttttcagcaa gcggaacagg
    25621 cgtgaggtat acgccccgtt ataggagtgc attgccgtgg tgtggctggc ggtgactcgc
    25681 gcgcccatgc cttcacggtg cgccagggca gcaacggttt cgacaaagcg cgactgctcg
    25741 tcatcgatct catcacagtg aacgtcgatg agacggtcgt atttttgcgc cagggcgaag
    25801 gttttatgca gcgactccac gccgtattca cgggtaaatt caaaatgcgg aatcgccccc
    25861 actacatctg cccctaagcg taacgcctct tccagcaatg cttcaccgtt gggatacgac
    25921 aaaatccctt cctgcgggaa ggcgacgatt tgtagatcaa tccacggcgc aacttcctgc
    25981 ttcacttcca gcattgcttt cagcgcagtt agcgttgcat ccgaaacatc gacatgggta
    26041 cgcacatgct gaatgccgtt ggcaatctgc catttcagcg tttgccatgc gcgttgtttc
    26101 acgtcgtcat gggttaataa cgctttgcgc tcggcccagc gttcaatgcc ttcaaacagc
    26161 gtgccggact gattccagtt cggttgtccg gcggtttgcg tggtgtccag gtgaatatgt
    26221 ggctccacaa acggcggtat aactaaacct tgttcggcat ccaggctgtt ttcagttatg
    26281 ggcatcacgc cggattgcgc atcaatggcg ctgatttttc cgtcctgcag atgaatctgc
    26341 cacagcccct ctttgcctgg taaccgggcg ttaataattg tttgtaaagc gttattcgac
    26401 actgttaacc tccacatgcg tcattgctgc tgtcgtttta cgattcaaaa tcggattaag
    26461 gatcagatag ctcagcgcgc cacctaatac cgcgttgacc ggaacaattc ccggtaacca
    26521 gtggcctgcg gcaatcccca gcgcgaccgc cagaatcgcc acccaattga cactcatcat
    26581 acgcgtggtc gcaaagtgct catagcggcg acggttcatc agatagtcgg cgatgatcac
    26641 gccccctact ggaggaatag ctgccgaaag gaaggtcaac cagccgacaa aattgttata
    26701 cagccataat gcgcagaccg taccgataat accgttgatt accgaaaggg ttttgctcga
    26761 cataccggta atgttggcga aacctaaacc cgacgcatag agtgcgttat cgttggtggt
    26821 ccagatattc agccccagca ccacaatcgc aggcagcagc aggccctgag caatcatcac
    26881 atcagagata tccgccatgc ccagtgccgc agcgcccgct gcaccgaaaa taaacatcaa
    26941 cgagttgccg aggaaaaagg ccaccatcgc caccagcacc gccagtttgg cattgcgacc
    27001 aaaccggaca aagtcagcgg tgagcgtacc cgcactgata aatgacccca caaccagcgc
    27061 cagcgcgaca ttgaaatcta acggttgtgc gggaacgacc gcttttaatg cgtccaggcc
    27121 gcccatgccg ttaacagcca gccacacgga ataaccgccc aggcaggcga tagccggaac
    27181 cgcaatcacc gaaagaaccg tcagcgccga aatgccaaaa aagacggtga cggtcatcag
    27241 taaaccggaa acggcaatca gcaaattaat atccagcccg gttgccttac ccaccggaat
    27301 ggcaaacatc gccacaccga cgccaaacca gccaacctga gttccgccca gtagcagtga
    27361 aggcagccat gagcctttaa caccaaacga gaagcgagca agaagatgag tggtcaggcc
    27421 ggtttttgcg ccaatgtaac cgagaaatga agtgtaaata ccgaggagaa gattaccgat
    27481 gagaactgcg aggaagaaat catgatagct aagaccggtt ccgagagtgc cgccggtcca
    27541 catactggcg gaaaagaagg ttaatcccag catgacgaac gtcaatgcca ataccccttt
    27601 ccgcgccgac tgcgggactg gcccctggct aaagttgtta tcttgcgaca cgaaattccc
    27661 cctttttgct gtttgaaacc caaaaaatcc gccgcattct attcatctga agataaaaag
    27721 caatcgtttt cgtggggaaa tatatttttt atatgaatga gtgcaaaaag gtgggggaaa
    27781 aattatcggt gtaaaaacag tgggtaggca taataagacg cggtaagcgt cgcatcaggc
    27841 atcggcgcat ggtgtcggat gcgccacgcg acgcatccga caatacctta ctcttccatc
    27901 gcctggcgga tctgatccaa cgacgcagga tcatcaatgg tcgtcagatc cccaggatcg
    27961 cgtccttcgc aaatcgcctg gatcgtgcgg cgcagcattt ttccggatcg cgtttttggc
    28021 aattgcgaga caaaccagac gtgcgccggg cggccaaagt tgccaatctg gctgtccacc
    28081 agcgccataa tcgccttctc ttgcgagtgc gccacatcac gatcttccag actgtcgctc
    28141 tctttcggaa tgacaaacgc caccgccacc tgccctttca gcgcatcttt caccccaacc
    28201 accgccactt cggcaacgcc cggatggctg gagatactct cttcaatctc gcgcgtcccc
    28261 agccgatgcc cggcaacgtt aattacatcg tcagtgcgcc cgagaataaa gtgataaccg
    28321 tcagcgtcac ggatgcccca gtcaaaggtg gcgtacaccg ggcgggaaaa cagcgaccag
    28381 taagtcttca caaagcggcc gtcgtcgccc cagatggtct gaatacaccc cggcggcagc
    28441 ggcccttcca ccaccagcat ccctttctcg ttgacgccac acggttcgcc ggtgacttca
    28501 ttaagcaact gcacgttata gccatacatc ggcacaccgg ggcttcccag acgcgtcggc
    28561 ctgtcgtcca gaccgcgagc aatcgccata atcggccagc cggattcggt ctgccagtag
    28621 ttgtcgatga ccggcacatc cagcgtattg ctcacccaac tggcggtcgg ctcgtccagc
    28681 ggttctccag ccagatagag cacttccagc gacgagagat cgtgtttgcg aatttcagcg
    28741 gtagggaatt ttttcagcac gcgaatggcg gtcggcgctg agaacatccg gctaacctga
    28801 tatttctcga cgattttcca ccacacgccg cagtccggcc aggtcggcaa tccttcgtaa
    28861 acgatagtcg ccatccccgc cagcagcggc gcgtaaacga tatacgaatg ccccaccacc
    28921 cagccgatat ccgatgcgca aaagaacacg ctgcccgctt tgccgccaaa aatggtgtcc
    28981 atcgaggtcg ccagcgccac cgcatatccg ccgacgtcac gctgcacgcc tttaggtttg
    29041 ccggtcgtgc cggaagtgta gagaatgcag gaggtttcgt tggattccag ccacgccact
    29101 ggtacccgcg cgccgatgtg ttgatagcgc aacgacgcga aatcgacatc ccgcccgctg
    29161 acgcgcgcca ttttcgccag tccgcgatcc accagcaaaa catggcgtgg ctggtgctgc
    29221 gcctgactta tcgcatcgtc gagcaatttt ttatagggaa tgattttgcc accgcgcgct
    29281 ccggcatcag ccgaaacaat cagcaccggt ttagcatcat caattcgcgc cgccacgctg
    29341 tgcgaggcaa atccaccaaa caccaccgag tgaatagcgc caatgcgcgc gcaggccagc
    29401 agagtaatat gcgcttcggc aatcatcggc atatacacca gcacccgatc gccacgctgc
    29461 acgcccaatg aacgcaacat tgaggccacc gcgttcactt cgtcatgcag ctgacgaaag
    29521 gtaaaggtgc gctcttcttc tgtttccgaa gagacggcaa tcagcgccag cgcctctggc
    29581 tgtttctcca gccagcggtc gatagcgttg tggcacaaat tggttcggcc ttcacaaaac
    29641 caacgggcaa acggcggatt gctgtggtcg agcgtttgcg taaagggcgt ctgccagtca
    29701 atacgccggg cctgctcggc ccagaactgc tccggttcgt taatcgaacg ctgataaaat
    29761 tcgctaaaag acataatgct ctcctgttga acttacgcct taccgccgtt tacttaaatg
    29821 acgtacaggt cgagatactc attgaccggc atctgttcca ggcgagttct gtcgagagaa
    29881 acctccagaa tgcgctgctg ttggcgagtc gggaactggc gcgcgagatt gattttgaat
    29941 ttatcgacca gtttcggaat accatcctga cggcggcgag catgaccaat ggggtactcc
    30001 accaccactt cttcaaatcg tgtgccgtcg gtgaactcaa gggtaatggc attggcgatg
    30061 gcgcgttttt ccgggtcgtg gtagtcagcg gtaaatgccg gatcttcaaa gcaattgatc
    30121 ttctcgcgca gggcgtcgat gcgtttatct tgcgccacgt tgtcctcgta atctgccgcc
    30181 gttaagcgcc cgaacagcag cgggatcgcc accatgtact gaatgcagtg atcgcggtct
    30241 gccgggttat tgagcggtcc ttttttgtcg atgatgcgaa tacaggcttc gtgggtgcga
    30301 atggtcactt tttcgatatc cgccgccgtt ttgcctgctg cctgcatctg ttcatagagc
    30361 gtcatcgctg cttcaactgc cgtctgggag tggaactccg ccgggaagga gattttgaac
    30421 agcacatttt ccataacgta ggaaccgtac gggcgctgga agcggaacga ttcaccttta
    30481 aaggagacgt cgtagaagcc ccacaccggc gcagtcaggg ctgacgggta acccatttcg
    30541 cccgttttcg ccatcagtgc cagacgtacc gcgcgggaag tggcatcgcc cgccgcccag
    30601 gatttacgcg tgccggtgtt cggcgcatgg cgataggtgc gcagcgactg accgtccacc
    30661 cacgccagcg aaacggcgtt gagaatttcc tcgcgggtca ggccgagcat ttcggcgacc
    30721 acggcggtgg aagccacttt cactaacaga acgtggtcga ggccgacgcg gttaaaggag
    30781 ttttccagcg cgatgcagcc ctgaatttca tgggctttga tcattgcggt cagcacctgt
    30841 ttcatggtca acggcgcttt gccgctggcg accgcgttgc gcgaaagcca gtccgccgtt
    30901 gccagaatgc cgccgaggtt gtcggaagga tggccccact ccgccgccag ccaggtatcg
    30961 ttgaaatcga gccagcggat catcgcgccg atgttaaatg ccgcctggac ggggtcgagc
    31021 tggaactgag ttccggggac gcgcacgccg ttgggtacga cggtgccggg aacaattggc
    31081 cccagcagtt ttttacaggc cgggtattcg agagcttcca gaccgcagcc gagcgtgtcg
    31141 agcaggcagt aatgtgcggt gtcgtaggcc actttagagc tgatttcgta gttcatgacg
    31201 taatcgacga tatcaacgat ttcacgatca aattccgggc ggatgttgtt gatttgagct
    31261 gacattgggt acgtttcctt attgttattc gtagaggttt actggcgctt atccagcgcg
    31321 acaaacgggc ggtcttccgg tccaacataa ttggcggaag gacggataat tttgttgtcc
    31381 tgacgttgtt cgataatgtg cgccgcccag ccggtgacac gggcgatgac aaacagtggt
    31441 gtgaacatct cggtgggaac gcccatcatg ttgtaggaaa cagcagagaa ccagtcgaga
    31501 ttggggaaca tctttttgct ctcccacatc accgtttcca ggcgatcggc gatgttgtac
    31561 atcttcaacg agccgccttc ctgcgagagc tgcttcgcca cacgtttgat cacctggtgg
    31621 cgcgggtcgg cgatggtata aaccggatga ccaaaaccaa tgaccacttc tttgttttcc
    31681 acccgcttgc ggatatcggc ttcggcttcg tccggcgttt cgtagcgttg ctggatctcc
    31741 agcgacactt cattcgctcc gccgtgtttc ggcccgcgca gtgcgccaat cgcgccaata
    31801 atcgcggaat acatatcaga gccagtgccc gcaatcaccc gactggtaaa ggtggaggcg
    31861 ttaaactcgt gttcggcgta cagcactagc gagatatgca tcgccttttc ccagctttgc
    31921 gatggctttt cgccgtgcag cagatgcagg aagtgaccgc cgatggagtc gtcatcggtt
    31981 tccggctgga tgcgttcgcc gttgtgactg tagtgatacc aataaaggag aatcgagcta
    32041 agtgatgcca gcagtttgtc ggcaatatcc cgcgcgccag agacagtgtg cccctctttt
    32101 tctggcagtg tgcagccgag cgcggaaacg ccggtgcgca tgacatccat cgggtgcgac
    32161 gccgccggta aggcttccag cacggtacgc acattagctg gtaaaccacg cagggctttc
    32221 agtttcgttt tgtaagcggc gagttcgtca cgggtcggca gtttgccgtg gatcagcagg
    32281 tgcgccactt cttcaaattc gcaatgttcc gccagatcaa gaatatcgta gccacggtaa
    32341 tgcaggtcat tgccactttt acccacggtg cagagcgccg tattgcccgc cggaacgcca
    32401 gaaagtgcca ccgatttttt cggtttaatg acatgggtac tgttttgcag gatcgttgtg
    32461 tcgctcatat tgtcctcgtc atttgtaggg tcgagatatt tttcgttgtg ttttatttca
    32521 cctggctacg ggcaaacagg tcgtcgagct tctcttcgta ctggtagtag ttgatgcttt
    32581 cgtacagttc gttgcgggtc tgcatggtgt cgatgacgct tttctgcgtg ccttcctggc
    32641 gcaggacgtt gtagacatgt tcagcggcgc ggttcatggc gcggaacgct gaaagcgggt
    32701 acagcgccat tgcgacatgg gcgctgcgta attcgtcggt ggtaaacagc ggcgtggcac
    32761 caaattcggt aatgttggcg aggattggca cctgaaccgc atcggcaaac tggcggtaca
    32821 tggcgagttc ggtaatcgcc tccgggaaca acatctcggc acccgcttca acataggcct
    32881 gtgcacgctc gatcgccgca tccagcccct ctaccgccag cgcatcggtg cgcgccatga
    32941 tcacaaaatc aggatcggtt ttcgcatcca ccgccgcgcg gatccgatcc accatctcct
    33001 ctttcgagac gatcgctttg ttcggacgat gaccgcagcg tttcgcacca acctgatctt
    33061 caatatgcaa tcctgccgca ccggctttaa tcatcgattt cacggtgcgc gccacgttaa
    33121 aggccgaaga accaaaaccg atatccgcat ccaccagcag cggcagcgaa caaacgtcgg
    33181 tgatacggcg aatgtcggtc agcacatcat caagggtaga aataccgaga tcgggcagcc
    33241 ccagcgaacc tgccgccacg ccaccgccag aaagataaat tgcctgatat ccggcacgct
    33301 gcgccaacag cgcatgatta gcgttgatgg tgccaacaat ctgcaatgga ttttctttag
    33361 tcagtgcagc gcgaaacgct ttacctggag agtgtagaga catagcccat cctttgttat
    33421 caacttgtta ttttcgttaa taaaacaaag caaggggtgt gccacaattg ccttaataaa
    33481 attaaatgtt taatttcaat ttgttagaga taacgtcatg aactaatccg caaatatgcg
    33541 tttcagttaa cgtttcaggc gatgtttcac cgcgtttcat tgcaacaatt atgaaacaag
    33601 actaaaccca atattcggtt tcttaacttt gcggtgcgct atggcacatc caccacggct
    33661 taatgacgac aaaccggtta tctggacggt atctgtaacg cgcctgttcg agctgtttcg
    33721 cgatatcagc ctcgagtttg atcacctggc gaacattacc cctattcagc ttggctttga
    33781 aaaagcagtg acctacatcc gcaagaaact ggcaaacgaa cgctgtgacg ccatcatcgc
    33841 tgctggctct aacggcgcgt acctgaaaag ccgcctgtca gtgccagtta ttttgattaa
    33901 accgagcggc tacgatgtgt tacaggcact ggcaaaagcc ggaaaactca cctcttctat
    33961 cggcgttgtc acctatcagg aaaccattcc agcgctggtg gcgtttcaaa aaacctttaa
    34021 tctgcgcctc gatcaacgta gctacattac cgaagaagac gcccgcgggc agattaacga
    34081 gctgaaagct aacggcactg aagcggtggt cggcgcgggg ctgattaccg atctggcaga
    34141 agaagccgga atgaccggaa tttttatcta ttccgccgcc accgtgcgcc aggcgttcag
    34201 cgatgcgctg gatatgacgc gcatgtcgtt acgccataac actcacgatg ccacccgcaa
    34261 cgccctgaga actcgttacg tgctgggcga tatgctcggt caatcaccac agatggaaca
    34321 agtacggcag actattttgc tgtatgcccg ctccagtgca gcggtgttga ttgaggggga
    34381 aacggggacg ggcaaagagc tggcggccca ggcgattcat cgggaatatt ttgcccgcca
    34441 cgatgcgcga cagggcaaaa agtcgcatcc gtttgttgcc gtcaactgcg gggcgatagc
    34501 cgaatcgctg ctggaagcgg agctgtttgg ctatgaggaa ggggcgttta ccggctcgcg
    34561 acgcggaggt cgcgccgggc tgttcgaaat tgcccacggc ggtacgctgt ttctggatga
    34621 gattggcgaa atgccgctac ctttgcagac tcgcctgtta cgggtgctgg aagaaaaaga
    34681 ggtcacccgc gtcggcgggc atcagcctgt tccggtagat gtacgggtca ttagcgccac
    34741 tcactgcaag ctggaagaag atatgcagca aggacgtttt cgccgtgatc tgttttatcg
    34801 gctgagtatt ttgcgtctgc aattgccacc actgcgcgag cgggtggcgg atattcttcc
    34861 gctggcggaa agctttttga aagtgtctct ggcggcgctc tccgccccgt tttctgccgc
    34921 attacgccag ggattacagg caagcgaaac cgtgctggtg cactacgact ggccaggtaa
    34981 tattcgtgaa ctgcgcaata tgatggagcg actggcgcta tttttaagtg tggaaccgac
    35041 gccggattta acgccgcaat ttttgcagct gctactgccg gaactggcgc gcgagtcggc
    35101 gaaaactccc gctccacgct tactgacacc acaacaggca ctggagaaat ttaatggcga
    35161 taaaacagca gcggcgaatt atttaggcat cagccggacg acgttctggc ggcggctgaa
    35221 aagctgaact gccgaatgca gcgcgaacgc tttatccggc ataaatttca atagatgatc
    35281 accgataaac ttacgcatcg gggatgttta catagggttc agaattactt cttcttataa
    35341 atatttgccg tgccgtggat cttattgtca gtttgaccgg aggtcagtac caacacatca
    35401 gccccttttt catccgcttt tttgatcaaa tcttctttcg cgtctgcagt cgacatttca
    35461 ttgctggttg aaatatcacc tattttttca tactgcgatt caactttttc aaattccgct
    35521 ttggtcatca attcagcggc atagacattg gtaacggcta acgctaacgc accgactaac
    35581 attttagaga ttattttcat attacggctc cagagttgtt atcacactat cacccaccca
    35641 cattattggg ttgattaata attagttcag tcagggcata aacgccagta accggaaata
    35701 agacactttg tgatctctct cgcacccgat tatttttggt agaaatatta ttaagttact
    35761 tcacttattt atggatgaaa tcactatgga tcctttgcac gccgtttacc tgaccgtagg
    35821 actgttcgtg attacttttt ttaatccggg agccaatctc tttgtggtag tacaaaccag
    35881 cctggcttcc ggtcgacgcg caggggtgct gaccgggctg ggcgtggcgc tgggcgatgc
    35941 attttattcc gggttgggtt tgtttggtct tgcaacgcta attacgcagt gtgaggagat
    36001 tttttcgctt atcagaatcg tcggcggcgc ttatctctta tggtttgcgt ggtgcagcat
    36061 gcgccgccag tcaacaccgc aaatgagcac actacaacaa ccgattagcg cctcctggta
    36121 tgtctttttt cgccgcggat taattaccga tctctctaac ccgcaaaccg ttttattttt
    36181 tatcagtatt ttctcagtaa cattaaatgc cgaaacacca acatgggcac gtttaatggc
    36241 ctgggcgggg attgtgctcg catcagttat ctggcgagtc tttcttagtc aggcgttttc
    36301 tttgcccgct gtgcgtcgtg cttatgggcg tatgcaacgc gttgccagtc gggttattgg
    36361 tgcaattatt ggtgtattcg cgctacgcct gatttacgaa ggggtgacgc agcggtgagc
    36421 agataattta ctctctacgt aatcaacctg atttgccgaa tcccggccat cggcgatgat
    36481 agcccccgta atgcgtggtc cttatttccg ataagtttac ttgagtcttt agcttcccat
    36541 ttcagcccac tctcccctgt gtgggctttt ttcattggtc tgacccgtcc tgaatagcca
    36601 aacaatggca atgttccact ttgcagggat gataataagg ggatctgatt cagaagagtt
    36661 ttgaaaagtt ggaccgccat cgaggcctcg aaccccgtac catacaacaa tcctggttgt
    36721 cgtctgctct tcctgatgag ctaatggcgg tttttgatat gagccttaca gcatattttc
    36781 tgtcaggctt gagtgcgatg ctaaggtaca gaaaagaccc cacacaaaca tgatggaatt
    36841 atgatttttt tcatatgaat gtcaacccac acattcacaa aacatctgca tgagatcatt
    36901 ttttatacac cgcatagctg cgaaaccact gctggtgctt ttttaagcga agcgtcgccg
    36961 atcacaacaa aatatcggcg aaaaaggcgg tggaatgcca tttggcatac ggatatacag
    37021 atgaaatgat tacctgcttt gcgattcact ctctggtgga ataacaactt gcaggtggaa
    37081 ccctttctga actaaagagt gtagcggatt ttaacgtgct tatttacagg ataatatctg
    37141 tcacctaatt cctgcactct attcgatatc cattcattta atcgtgcatc atctctgtat
    37201 gtttttacgt ccagaacaga gagcaataac atcaccagtg attcaggaaa aacagggtag
    37261 atatttttta agtgtaataa taatccatgc gcaagcgcct gcttaatggg cgcgtctgtc
    37321 tccggaatag acgacatttc gcgaagccat ttttccacat gcatacactc tttcccttta
    37381 gggaaaagtc tcagcaaagg catatggtgt tcaagaagaa tgcatttgca atctgttcgc
    37441 actgaatcga taaactgcac taatttctta agcccggtaa agcgtccagc ctgcacattt
    37501 gctttaatat caagaaacgc atcatcctgg tcatataccc aaactgtccc tttttgattt
    37561 tcaagtatgc gaaaaacaaa cttaaccggg tgatcagggt aatccatcca gtcataaaat
    37621 ccagggaaat cattaaactg tacatttatg gtgagtgcgt tatcgtctga ccactcacag
    37681 tctcgtacgg cagccatgag atgaatgagt ttgtcgtcat cgactaaatc atcacgacaa
    37741 tcataaggat aatcataata ttgtttaatc aaaaccttga atatatcgac acaggaaacc
    37801 agggagcttt tgatatcctc tggtaactga ttgtttttaa ccgtattaag tatgctttgt
    37861 tgcgtatatg gcatcaaatt attgtgcgga gcttttaatg atatcatcgc gatctctctt
    37921 aaataaaaat aaacgtcgca gataataaag ctctgattaa accaggcact atcagaaatc
    37981 gctca
//
LOCUS       DAPPNJ010000019        37892 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000019 DAPPNJ010000000
VERSION     DAPPNJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.019"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(82..369)
                     /locus_tag="RPM73_000733"
     CDS             complement(82..369)
                     /locus_tag="RPM73_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDX6519125.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDDDIIRLVAER"
     gene            complement(444..587)
                     /gene="mgrB"
                     /locus_tag="RPM73_000734"
     CDS             complement(444..587)
                     /gene="mgrB"
                     /locus_tag="RPM73_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDX6519126.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            746..985
                     /locus_tag="RPM73_000735"
     CDS             746..985
                     /locus_tag="RPM73_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519127.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1128..1919)
                     /gene="kdgR"
                     /locus_tag="RPM73_000736"
     CDS             complement(1128..1919)
                     /gene="kdgR"
                     /locus_tag="RPM73_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDX6519128.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2096..3469
                     /locus_tag="RPM73_000737"
     CDS             2096..3469
                     /locus_tag="RPM73_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519129.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3515..4396)
                     /gene="htpX"
                     /locus_tag="RPM73_000738"
     CDS             complement(3515..4396)
                     /gene="htpX"
                     /locus_tag="RPM73_000738"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDX6519130.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4588..6636)
                     /gene="prc"
                     /locus_tag="RPM73_000739"
     CDS             complement(4588..6636)
                     /gene="prc"
                     /locus_tag="RPM73_000739"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDX6519131.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKSELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6656..7354)
                     /gene="proQ"
                     /locus_tag="RPM73_000740"
     CDS             complement(6656..7354)
                     /gene="proQ"
                     /locus_tag="RPM73_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDX6519132.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7451..7948)
                     /gene="msrC"
                     /locus_tag="RPM73_000741"
     CDS             complement(7451..7948)
                     /gene="msrC"
                     /locus_tag="RPM73_000741"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDX6519133.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8078..9361
                     /gene="yebS"
                     /locus_tag="RPM73_000742"
     CDS             8078..9361
                     /gene="yebS"
                     /locus_tag="RPM73_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDX6519134.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGESLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9330..11963
                     /gene="yebT"
                     /locus_tag="RPM73_000743"
     CDS             9330..11963
                     /gene="yebT"
                     /locus_tag="RPM73_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDX6519135.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12043..13482
                     /gene="rsmF"
                     /locus_tag="RPM73_000744"
     CDS             12043..13482
                     /gene="rsmF"
                     /locus_tag="RPM73_000744"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDX6519136.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKEVWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13601..13837
                     /locus_tag="RPM73_000745"
     CDS             13601..13837
                     /locus_tag="RPM73_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDX6519137.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13942..14133
                     /locus_tag="RPM73_000746"
     CDS             13942..14133
                     /locus_tag="RPM73_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDX6519138.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14134..14790)
                     /gene="pphA"
                     /locus_tag="RPM73_000747"
     CDS             complement(14134..14790)
                     /gene="pphA"
                     /locus_tag="RPM73_000747"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDX6519139.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15186..15527)
                     /locus_tag="RPM73_000748"
     CDS             complement(15186..15527)
                     /locus_tag="RPM73_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDX6519140.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15540..16412)
                     /gene="copD"
                     /locus_tag="RPM73_000749"
     CDS             complement(15540..16412)
                     /gene="copD"
                     /locus_tag="RPM73_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDX6519141.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16416..16790)
                     /gene="yobA"
                     /locus_tag="RPM73_000750"
     CDS             complement(16416..16790)
                     /gene="yobA"
                     /locus_tag="RPM73_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDX6519142.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16929..17159
                     /gene="holE"
                     /locus_tag="RPM73_000751"
     CDS             16929..17159
                     /gene="holE"
                     /locus_tag="RPM73_000751"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDX6519143.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMSVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17261..17917
                     /locus_tag="RPM73_000752"
     CDS             17261..17917
                     /locus_tag="RPM73_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDX6519144.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17941..18603
                     /gene="exoX"
                     /locus_tag="RPM73_000753"
     CDS             17941..18603
                     /gene="exoX"
                     /locus_tag="RPM73_000753"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDX6519145.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18600..20660)
                     /gene="ptrB"
                     /locus_tag="RPM73_000754"
     CDS             complement(18600..20660)
                     /gene="ptrB"
                     /locus_tag="RPM73_000754"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDX6519146.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHREFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20869..21528)
                     /locus_tag="RPM73_000755"
     CDS             complement(20869..21528)
                     /locus_tag="RPM73_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDX6519147.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21855..22211)
                     /locus_tag="RPM73_000756"
     CDS             complement(21855..22211)
                     /locus_tag="RPM73_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDX6519148.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22278..22568)
                     /gene="yebG"
                     /locus_tag="RPM73_000757"
     CDS             complement(22278..22568)
                     /gene="yebG"
                     /locus_tag="RPM73_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDX6519149.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22702..23880
                     /gene="purT"
                     /locus_tag="RPM73_000758"
     CDS             22702..23880
                     /gene="purT"
                     /locus_tag="RPM73_000758"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDX6519150.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23936..24577)
                     /gene="kdgA"
                     /locus_tag="RPM73_000759"
                     /gene_synonym="eda"
     CDS             complement(23936..24577)
                     /gene="kdgA"
                     /locus_tag="RPM73_000759"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDX6519151.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24614..26425)
                     /gene="edd"
                     /locus_tag="RPM73_000760"
     CDS             complement(24614..26425)
                     /gene="edd"
                     /locus_tag="RPM73_000760"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDX6519152.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWRKGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26660..28135)
                     /gene="zwf"
                     /locus_tag="RPM73_000761"
     CDS             complement(26660..28135)
                     /gene="zwf"
                     /locus_tag="RPM73_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDX6519153.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28473..29342
                     /gene="hexR"
                     /locus_tag="RPM73_000762"
                     /gene_synonym="yebK"
     CDS             28473..29342
                     /gene="hexR"
                     /locus_tag="RPM73_000762"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDX6519154.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29470..30912
                     /gene="pyk"
                     /locus_tag="RPM73_000763"
     CDS             29470..30912
                     /gene="pyk"
                     /locus_tag="RPM73_000763"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDX6519155.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31043..32014)
                     /gene="lpxM"
                     /locus_tag="RPM73_000764"
                     /gene_synonym="msbB"
     CDS             complement(31043..32014)
                     /gene="lpxM"
                     /locus_tag="RPM73_000764"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDX6519156.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32134..33456)
                     /gene="mepM"
                     /locus_tag="RPM73_000765"
     CDS             complement(32134..33456)
                     /gene="mepM"
                     /locus_tag="RPM73_000765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDX6519157.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33472..34404)
                     /gene="znuA"
                     /locus_tag="RPM73_000766"
     CDS             complement(33472..34404)
                     /gene="znuA"
                     /locus_tag="RPM73_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDX6519158.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34483..35238
                     /gene="znuC"
                     /locus_tag="RPM73_000767"
     CDS             34483..35238
                     /gene="znuC"
                     /locus_tag="RPM73_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDX6519159.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35235..36020
                     /gene="znuB"
                     /locus_tag="RPM73_000768"
     CDS             35235..36020
                     /gene="znuB"
                     /locus_tag="RPM73_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDX6519160.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36167..37177)
                     /gene="ruvB"
                     /locus_tag="RPM73_000769"
     CDS             complement(36167..37177)
                     /gene="ruvB"
                     /locus_tag="RPM73_000769"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDX6519161.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37186..37797)
                     /gene="ruvA"
                     /locus_tag="RPM73_000770"
     CDS             complement(37186..37797)
                     /gene="ruvA"
                     /locus_tag="RPM73_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDX6519162.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9060 a         9797 c         9350 g         9685 t
ORIGIN      
        1 cagttgcacc atgatagtgc gtataatcag gatgaattga gtaacccttc aacgacgggg
       61 ttactcaaga ttgaggaagg attatcgttc ggcgaccaga cgaataatat cgtcgtcttc
      121 aaccgatttt tgactctcaa cggtcttttt atctgctttc tctggttcgt ttgcttcgca
      181 caaacgacgt aacagtgcat tttgacgttt ttgctgatcc agcaacgcct caagcagttc
      241 aatctgttcg ttagtccgag aactggcacg attaataaaa aaccacaaga tgagcccgat
      301 aagaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg aatttacaac
      361 ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg agaggaagaa
      421 aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc acaaattccg
      481 ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca aagcagcaag
      541 caagccaaca ccacgacaac cagaacgacc catcgaaact ttttcactcc aatctccgtt
      601 tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta actatgtcga
      661 tattcgtgct ttttcggaat gagtcgcttg cttcagcgca tattgccgct acgattaagc
      721 gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta tgctgatagc
      781 actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa gcaaagagaa
      841 tgccgcagga ttaggtttgc aatgtaccta tctgactgcc agaggcacca gcacggtaca
      901 atacttgcac actaaaagtg gtttcttggg gataacagat tgcccactgt tacgtaaaag
      961 caatatcgtg gtcgataatg gttaagggta ataaataacc agattctcac tcatgagtaa
     1021 aatattctta tagcaagcgc gtttaatata acgcaccttc aactttattt ttctcatgct
     1081 aaataaagaa cctgtctgtt tgatattccg acaggttctt actctcatca gaacggatag
     1141 tcgtgataac ccatttgggc agaaattttg cgcgctgcgg tatgcaacat tgcgacatat
     1201 tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg agatgctcaa accggcaatg
     1261 accacgccaa agcgatcaaa taccggtacc gcaatgcatc gcagcccttc ttcctgctct
     1321 tcgttatctt cgccataccc ctgctcgcgc acctggtcca gaacgggtaa taacgcttct
     1381 gtactggtga tggtccgctc ggtactgcgt ttatactcca cgccctcaag aatttgcttc
     1441 acttcatcga gatcgcgcca tgccagcagt accttaccaa tcgcggtgct gtacagcgga
     1501 ttacgacgcc caatccgtga atacatgcgc aaattgtaca tagagtcaat tttgtgaatg
     1561 taaacaatac tgtcttcgtc cagtgcgccg aggtggatag tttctttggt caggcgggag
     1621 atctcacgca tctggatatc tgcgctacga attaaatcga cgttttgtaa cgcgcgagcg
     1681 cccagttcaa acaatttcag ggtcagggaa tatttctccg actccccttc ctgcgccaca
     1741 taacctaagg ttttcatggt ctgtaaaaag cgataaacgg tgctttttga catcatgacg
     1801 cgctgcgaca gctcggttat ccctatttcg cgctcttcac ccagcgcctg caaaatgcca
     1861 aaaactttta gcacggaaga tacagaatca ggctgtttat ccagatctgc gttagccatt
     1921 tatcacctca ttgcgagtgt tttataaaaa tcagaactgt tttttattat aatttcgcac
     1981 cagggtggtc gcaatccatc ttttgccggt tagttacaat tctgcgacat ccaccgtgaa
     2041 tatcagtgct agaatcatac ccctgttgat tattcaccaa agatataaaa ttcctatgcc
     2101 aaaagttcag gccgacggcc tgccattgcc ccagcgatac ggtgcgatat taaccattgt
     2161 gattggcatt tcgatggctg tccttgacgg cgcaatcgcc aacgtcgccc tgccaacaat
     2221 cgccacggac cttcatgcca cgccagccag ttccatctgg gtagtgaacg cctatcaaat
     2281 cgccattgtc atctccctgc tctcgttttc gtttctgggc gatatgtttg gctatcgacg
     2341 tatttataaa tgcggtctgg tcgtttttct gttgtcttca ctgttctgcg ccctttctga
     2401 ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt ttcggcggtg cagcgttgat
     2461 gagcgttaat accgcactta tccgcctgat ctatccacaa cgttttctgg ggagagggat
     2521 gggcataaac tcgtttattg ttgccgtctc ttctgctgcc gggccgacaa ttgctgcagc
     2581 aatcctctcc atcgcatcct ggaaatggtt atttttaatc aacgtaccgt taggtattat
     2641 cgccctgctt ctggcgatgc gttttctgcc acccaatggt tctcgcgcca gtaaaccccg
     2701 tttcgacctg cccagcgccg tgatgaacgc gttaaccttc ggcctgctta tcactgcgtt
     2761 gagtggtttc gctcaggggc aatcgctgac gttaattgct gcggaactgg tggtaatggt
     2821 tgttgttggt attttcttta ttcgccgcca gctttctctt cccgtaccgc tgctaccggt
     2881 ggatttactg cgtatcccgc tgttttcact ttctatttgc acatctgttt gctctttctg
     2941 cgcacaaatg ctggcaatgg tttccctgcc cttttacctg caaaccgtgc tcgggcgtag
     3001 tgaagtcgaa acaggtttac ttctgacacc gtggccgtta gcaacgatgg tgatggctcc
     3061 gctggcaggc tatttgattg aacgcgtaca tgcaggattg ctgggtgctt tagggttgtt
     3121 catcatggct gcggggcttt tttccctggt tctgctgccc gcgtcacctg cggatatcaa
     3181 tattatctgg ccgatgatct tatgtggcgc tggatttggc ttattccagt cacccaataa
     3241 ccacaccatt attacctccg ctccgcgcga acgtagcggt ggggccagtg gcatgttagg
     3301 aacggctcgt ctactgggtc agagtagtgg cgcggcgctg gtggcgctga tgctaaatca
     3361 gttcggtgat aatggtacgc acgtttcgct gatggctgcg gctattctgg cagtgattgc
     3421 tgcctgtgtg agtggtttac gtatcactca gccacgatcc agggcataaa aaaagcgcgt
     3481 cgatcaggac gcgcttttta gtatttactt catattactt caggtattca cccgtacgca
     3541 gagcttcaat tcgtttatcc agcggcgggt gagtcatgaa caactcactg agcgatttcg
     3601 acttaccgtt aatgcagaaa gccatcatgc tggttgcttc ttgcggttca tagctggttt
     3661 tcaggcgctg cagcgcggca atcattttct cgcgaccaac cagttttgcc gaaccggcat
     3721 cagcatggaa ttcacgatga cgcgagaacc acatggtgat aatgctcgca agaataccaa
     3781 acaccagttc cagaaccgtt gcaaccgcaa agtagatcag cgggttgccg ttgctctctt
     3841 caccttcatc acgatttccg cccataaaac ccgcggcaag ctgcgccaga atacgggaaa
     3901 taaagataac gaaggtgttc accacgccct gaatcagcgt catggtgacc atatcaccat
     3961 tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc ttcatccggg ctcatgttct
     4021 gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg gcgcgcaccg gttgcaaaag
     4081 cgttgatgtc cggcgcatgg tagatagcca cttgcggcat agcgatccct gcctgacgag
     4141 cctgggttgc tacagtatta accagccaac gttccctttc gttacgcggt tgctcgatca
     4201 cttccccgcc aacagatcgt aatgccatcc atttggacat cagaagcgaa acgaaggaac
     4261 caccaaaacc gaacagcaag gccatgatca tcagcccctg aacgctgctc gactgtatcc
     4321 ctgtcaggct cagtaccagc ccgaaaacga ccattacggc caggttcgtt agcaggaaga
     4381 gcgcgattcg catcataatt ttctttttac ctcagtttaa caaaacgcaa tatgcgatac
     4441 ccacatcgta tgggttacgc gactattttc aagtctggat agtgcgtaag tcaccagaaa
     4501 gacacaactt tacattttgt agcatctgat ttacggcatc ttgccgctgt taaaaaatca
     4561 ggcacaattt cttgtgcctg attgatatta cttgacggga gcgggttgtt ccgcgggtct
     4621 ggctttttca agcttcgcca gatcgagtgc gatattcacc gtctcatcca gataaggatc
     4681 cggctcctgg taatctttag gtagatcatc cagcttcttc aactccggtt taccttcgcg
     4741 tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca tcttcattat tctctttctc
     4801 acgcacagcg tagttcagag aaacgatatt gcgcttgtcc ttcatagcgt tgaagcgcgc
     4861 gatatccttc atgatgttct ggaactcagg atctttcgcg atacgcgcat tatgttcctt
     4921 cagcagctcc ggcccaaagg ccgttaaatc gcctgatttc acataagtcg cggcatcaat
     4981 gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc gtttccgttt cttcattacc
     5041 cgtcggcatg atgatgtctg gcgttacgcc tttacgttgc gtactgccgc cgttaacgcg
     5101 atagaatttc tggatcgtgt actgcacaga acccagcgct ggccattcag gacgtaacat
     5161 ctgatcgtaa atacggttca atgaacggta ttgctgaacg gtgcctttac caaacgtcgg
     5221 ttcacccaca accagcgcac gaccgtaatc ctgcattgcc gcggcaaaga tttctgaagc
     5281 cgaagcactg aaacggtcaa ccagtaccac cagcgggcct ttatagaaca cctgcccgtc
     5341 ggtatcgctg tcttcacgaa ccttgccgtt gttatcgcgg acctgaacaa tgggacccgc
     5401 aggaataaac agacctgaga gcgataccgc ttcggtcagc gccccaccgc cattgctacg
     5461 caggtcgatg atcacactgc tgacattctg tttttccagt ttctgcagtt gaactttgac
     5521 atcgtctgtc aaacccacat agaagcccgg aatatccagc acgccgactt tctctttacc
     5581 gacggtcttc accgacattt taaccgcgcg gtcttcgaga cgaatacgtt cacgggtcaa
     5641 tgttacagtg cgggtcttgg tccctttacc agcaggtaaa atttccagac gaactttact
     5701 gcccttcggc cctttaatta aggcaaccac atcatcaaga cgccagccaa tcacgtcaac
     5761 catcggcttg cctgtttgac caacaccgac aattttgtca ccaacgctga tagctttact
     5821 cttcgctgcc ggaccacctg ccaccatcga attgataacg gtgtagtcat catccatttg
     5881 cagcactgcg ccaatacctt ccagcgacaa actcatttca gtgttgaact gttcggtatt
     5941 acgaggggaa agatagttgg tatgcgggtc gatttcacgc gcaaacgccg tcattgccag
     6001 cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga atggcaaatt tgtagcggcg
     6061 tgtcagggtt tcacgaattt ctttatccgt ttttcctgcc agcttcaggc ttaactcgtc
     6121 gaatttgact ttactgtccc acagcgcgtt caactcagcc tcgtttttcg gccagggcgc
     6181 tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa tccatcggct tttccagtac
     6241 cgacaaagcg tactggtaac gctcaaaacg gcgcttttgc gccagattgt agagatcgta
     6301 gaaaacgtcg agtttgcctg aacgcagttc atcgcctaac tcgcttttct ttttcgcgaa
     6361 ctgttcaaca tcgcttgcca gcagcacgtt gtggctgtaa tcgagcagat tcaggtagcg
     6421 gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac tggcgataat gagaacgggt
     6481 gaagcgcgac gttacgcgct cacttaccgt cgcatgctgc gtctcttcct ttaataccgg
     6541 aatttgatca gcacgcgtga tatcttctac agcgaaggtc tggcctgcta ttgcaagcag
     6601 gccagctaac gcggtaagcc taaaaaacat gttcatgcct ggcccggcct ccgtttcaga
     6661 acaccaggtg ttctgcgcgc acaatcaaag acatacccga attcagctgg acgcggacgc
     6721 cgtctttggt gatttctaat acggtggcat ccatcgcgtt ttgacccgct ttcaccttca
     6781 gagcttgtcc gacagtcaga gctgaaatgt cagaaaccgg ggtgtgctgt tcttcgcgag
     6841 gtgcttttac tgtttttggc gctttctcta ccggcttttg cgcacgaggt ttacgttcag
     6901 cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg ttcgcggcgc ggtgcgtctt
     6961 ctttctcacc agcagctgcg gcagcttcgc gttttttcgc ttgctgttca gcacgctgtg
     7021 cctgaacacg cgctttcgct tcttcaagct gcttgcgagc atgctctaca tgttgctcat
     7081 ccagctcacc gcatgggttg ccgtcaagat cgacacgcgt tgcgccgggt ttaacaccgt
     7141 aaagataacg ccagctcgaa gtgtagagac gtaaagcgga tcgtaattgc gttttgctca
     7201 ggttcatttc cccggcaaca cgatcgacca aatcctgaaa aataccgatt ttcagcggac
     7261 gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc ggccagaaac gcgattactt
     7321 ctttactgct attcaactta ggttgatttt ccatgaaatt tcctgattac aacggacgta
     7381 gccaacaagc gcaggcatga acaggcgtca ttataatgac gctaccagta aatgctacgt
     7441 tatccgttga ttatcctgcg acgctcgcaa agaatttttt gtaatccgtc gttgcaagca
     7501 ctttttcaag ctgtgccaca agctgacgta agccttgctc gtcctcgtct gtaaagcgac
     7561 cgaagacggt actatcgatg tcaagaacac caataatctg atttttcacc accagcggca
     7621 gaacaatttc agaattactc gccgcatcac aggcaatatg cccgtcaaac gcgtgaacat
     7681 cctcgatacg ctgcacttga ttgcgggcaa ccgcagtgcc gcacaccccg cgcccgacgg
     7741 gtatccggac acaggcaatt ttgccctgaa atggtccgag taccagtgta tcgtcctcaa
     7801 gcaaataaaa acctgcccag tttacgtcag tgagacgctc gtataacaac gcactggtgt
     7861 tcgcaagcgt tgccagaaaa ctggtttctc ccgccatcag cgcgttaaag tcgcgattta
     7921 aatccgcgta aaattctgtt ttgttcatta tataatcact tggttgtctt acctggaact
     7981 gccagcctat taaaataagc attaaatgcg ttaatgctca agatcattcc catcatgggt
     8041 taatattaat gttaattctt attacatttg gcacgtcatg gctcttaaca caccacaaat
     8101 tacgccgaca aaaaagataa cagtgaggtc aatcggcgag gaactgccgc gtggtgatta
     8161 ccaacgttgc ccgcaatgcg acatgctgtt tagcctgccc gagataaatt ctcatcagag
     8221 tgcctattgt ccgcgctgtc aggcaaaaat tcgcgacggg cgcgactggt cgctaacgcg
     8281 cctggcggca atggctttca ccatgctgtt gctgatgccg tttgcctggg gcgaatcgct
     8341 gttgcatatc tggctgttag gtattcgtat cgacgccaat gtcatgcaag gcatctggca
     8401 aatgaccaaa cagggcgata cgataacggg ggcgatggtc tttttctgcg ttattggtgc
     8461 ccccctcatt ctggtgtcct ccatagcata tttatggttt ggtaaccgac tgggaatgaa
     8521 tttacgtccg gtactgctga tgcttgagcg tcttaaagag tgggtaatgc tggatatcta
     8581 cctggtcggc attggcgttg cttctataaa ggtacaggat tatgcccata tccaggctgg
     8641 tgtcggcttg ttctcttttg tggcgttggt gattttaacg acggtgacgt tgtcacatct
     8701 taatgttgag gagctgtggg aacgatatta tccgcagcgc cccgctacgc gtagggacga
     8761 gaaacttcgt gtctgtcttg ggtgccattt taccggctat cctgatcagc gtggtcgctg
     8821 cccgcgttgc catatcccgc tacgcctgcg tcgccgtcat agtctgcaaa aatgctgggc
     8881 ggcgctgtta gcgtcaatcg ttttgttgtt acctgccaac ctgttgccta tttctatcat
     8941 ttatctgaac ggaggacggc aggaagatac aattctttcc ggaattatgt cgctggcaag
     9001 tagcaacatt gcggttgcgg gaatcgtgtt tatcgccagt attctggtac cgtttactaa
     9061 agtgatcgtc atgttcactt tactgttgag cattcatttt aaatgccagc aaggtttacg
     9121 cacacgcatt ctgttactgc ggatggtgac ctggattggt cgctggtcga tgctcgacct
     9181 gtttgtcata tctttaacca tgtcgctgat taatcgcgat cagatcctcg cttttactat
     9241 gggaccggct gcgttttatt tcggcgcagc ggtaattttg actattcttg ctgtggaatg
     9301 gctggacagc cgcttacttt gggatgcaca tgagtcagga aacgcccgct tcgacgactg
     9361 aagcgcagat taaaaataaa cgccgtatct cacctttctg gctgctgccg ttcatcgcgc
     9421 taatgattgc cggttggctg atttgggaca gttatcagga ccggggtaat accgtcacca
     9481 tcgactttat gtcggcggat ggtattgtcc cgggccgtac gcctgttcgt tatcagggcg
     9541 ttgaagtcgg aacagtgcag gatatcagcc tcagcgacga tcttcgtaag attgaagtca
     9601 aggtcagtat caagtccgat atgaaagatg cgctgcgcga agagactcag ttctggctgg
     9661 tgacgccaaa agcatcgttg gcaggtgtct ccgggctgga cgccctcgtc ggtggtaact
     9721 atatcggcat gatgccaggt aaaggtaaag agcaggatca ctttgtcgca ctcgataccc
     9781 aaccgaaata tcgactggac aatggcgatc tgatgatcca cctgcaagcc cccgatctcg
     9841 gttcgctgaa cagcggttca ttggtctatt tccgcaagat ccctgtggga aaagtctacg
     9901 actatgccat caatcccaac aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt
     9961 ttaccgacct ggtgaaaaaa ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg
    10021 tcagtatcag tggcgcgaag gtgaaactgg aaagtctggc ggcactggtt aacggtgcga
    10081 ttgccttcga ttcacctgaa gagtcgaaac ctgccgaggc ggaagatacc tttggtctgt
    10141 atgaagatct ggcccacagc cagcgtggcg taataataaa actggaactg ccgagtgggg
    10201 ccggattaac cgccgactcg acgccgttaa tgtatcaggg gctggaagtc ggacagctga
    10261 ctaaactgga tttaaatcct ggtggtaaag tcactgggga aatgaccgtt gatcccagcg
    10321 tcgttacgct gttacgggaa aatacccgca tcgaattacg caacccgaaa ttatccctta
    10381 gcgatgccaa tctcagcgcc ctgctgactg gcaaaacctt cgagttggta cccggcgatg
    10441 gcgagccacg caaagagttc gttgttgtgc caggcgaaaa agcactgctg catgaacctg
    10501 atgttctgac gctgaccctg accgcgccgg aaagttacgg tattgatgcg ggtcagccgc
    10561 tcattcttca cggcgtgcag gtaggccagg ttatcgatcg taaactcacc agcaaaggcg
    10621 tcacctttac cgtcgccatc gagcctcagc atcgagaact ggtaaaaggc gatagcaaat
    10681 ttgtcgtcaa cagccgtgtc gacgtgaagg tggggctgga tggcgttgag tttctcggtg
    10741 ccagcgcctc agaatggatt aacggcggga tacgtattct gccgggcgat aaaggcgaga
    10801 tgaaagccag ctatccactg tatgccaatc tggaaaaagc gctggagaac agccttagcg
    10861 atttacccac cacaaccgtg agtttgagtg cagagacgct gccggatgtg caggcaggat
    10921 cggtagtgct gtaccgtaaa tttgaagttg gtgaagttat taccgtgcgt ccgcgagcta
    10981 acgcgtttga tatcgatctg catattaagc cggagtatcg caaccttctg accagcaata
    11041 gcgtgttctg ggcagaaggc ggggcgaaag ttcagctgaa tggcagcggc ctgaccgtac
    11101 aggcatcccc gctctccaga gcattaaagg gagccattag cttcgataac ctcagcggtg
    11161 ccagcgccag tcagcgtaaa ggcgacaaac gaattctgta tgcttccgaa acagcggccc
    11221 gtgcggttgg tgggcagatt acgcttcacg ctttcgatgc cggaaaactg gcggtcggga
    11281 tgccaattcg ctatctcggt attgatattg ggcaaatcca gacgctggat ctgattaccg
    11341 cgcgcaatga agtacaggca aaggcggtgc tctatccgga atatgtccag acctttgccc
    11401 gcggtggtac gcgcttctca gtggtcacac cgcaaatttc ggcagctggc gttgagcatc
    11461 tagatactat cctccagccg tatatcaacg tcgaaccagg ccggggcaat cctcgccgcg
    11521 actttgaatt acaagaggcc actattactg attcgcgtta cctggatggc ttaagcatta
    11581 ttgtcgaagc accggaagcc ggttcgttag gtattggtac gcctgtgctg ttccgtggtc
    11641 tggaagtagg tacggttacc ggaatgacgc tggggacatt gtcagatcgc gtgatgattg
    11701 cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa caattccgtc ttctggttgg
    11761 catcgggtta cagtctggac tttggtctga cgggcggcgt agtgaaaacc ggcaccttta
    11821 accaatttat ccgtggcggc atcgccttcg ccacgcctcc gggtacgcca cttgcaccga
    11881 aagcccagga aggcaaacac ttcctgttgc aggaaagtga accgaaagag tggcgtgaat
    11941 ggggaactgc gcttcccaaa taatgcccac tgctccggcg tgcctgcgcc ggagcgttta
    12001 tgctaaactg cgcgcctgtt tttttgccag tggtacatgc tcgtggccca acacaccgtt
    12061 tatttcccgg acgcctttct gacacaaatg cgcgaagcta tgccttcgac gctctcattt
    12121 gatgattttc ttgccgcctg tcagcgcccg ttgcgccgca gcattcgcgt taatacgctg
    12181 aaaatctccg ttgctgattt cctgcaatta accgctcctt atggctggac gcttacgcca
    12241 attccgtggt gtgaagaagg tttctggatt gaacgcgacg atgaagatgc cttgccattg
    12301 ggtagtaccg ccgagcattt aagtggcctg ttttatattc aggaagccag ttcaatgttg
    12361 cccgtcgccg ccttgtttgc tgacggtaat gcaccacagc gggtgatgga tgtcgctgcc
    12421 gcgcccggct ccaaaacgac gcaaattgcc gcgcggatga ataacgaagg ggcgatcctt
    12481 gccaatgagt tttccgccag tcgggtaaaa gtgttacatg ccaatatcag ccgctgtggc
    12541 atccgtaatg ttgcgctcac gcattttgat ggccgtgtat ttggtgcggc agtgccagaa
    12601 atgttcgatg ccattttact ggacgctccc tgctccggcg aaggcgtggt gcgtaaagat
    12661 cccgatgcgc taaaaaactg gtcaccagaa agcaatcagg aaatcgcagc tacccaacgg
    12721 gaactgatcg acagcgcctt tcatgcatta cgccctggcg gtacgctggt ttactcgacc
    12781 tgtaccttaa accaggaaga aaacgaagcc gtttgcctgt ggctgaaaga gacttacccc
    12841 gacgcagtag agtttttacc gcttggcgat ctcttccctg gtgcaaacaa agcgctgacc
    12901 gaggaaggct ttttgcatgt tttcccacaa atttacgact gcgaaggctt cttcgttgct
    12961 cgtctgcgta aaactcaggc gatccccgcc ttacccgccc caaaatacaa agtgggcaat
    13021 ttcccgttca gcccggtgaa agatcgcgaa gctggacaaa ttcgtcaggc ggctgcaggt
    13081 gttggcttaa actgggatga aaacctgcga ctctggcagc gcgacaaaga agtgtggttg
    13141 tttccggtag acattgaagc cctgatcggt aaagtccgat tttctcgcct ggggattaaa
    13201 cttgccgaaa cgcacaacaa aggttatcgc tggcagcatg aagcggttat tgcccttgcc
    13261 tcccccgaca atgtgaacgc ttttgaactg acaccgcagg aagcggaaga gtggtatcgc
    13321 gggcgcgatg tttacccgca agccgcgcca gtggcggatg acgtgttggt tactttccag
    13381 catcaaccga ttggtttagc caaacggatt ggttcgcgat tgaaaaacag ctatccgcgt
    13441 gaactggtgc gcgatgggaa actttttacc ggtaacgcct gacagcgcag aaaaaaaagc
    13501 gcactttttg actggcacat tcggctgcct caactaggct gaaaaatggt gcgatcggac
    13561 tggtcgtacc acaatcggca gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat
    13621 aggcgctttt gaaatcgacg acggcgaatt acacggtgaa tcgccgggtg atcgaacgtt
    13681 aaccattcct tgtaaatctg accccgattt atgtatgcaa ctcgatgcct gggatgctga
    13741 aaccagtatc cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga
    13801 tcaacaatct gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg
    13861 gcgggttatt tgtcctggtt attcccccgt tgtaaaatct ctcctaaact taacggtacg
    13921 gcaccacact tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc
    13981 ggttgtgaag atattgttgt ggatgtctac aacacggaac agcagtgtct ttattctatg
    14041 agcgatcaac ggatccgcca tggcggttgt tttccgattg aggattttat agatggtttc
    14101 tggcgacctg cacaggagta cggtgatttt taattattgc aattgcacaa gagtcagttc
    14161 gcccccaaag acagcgccgg tatcaatata atgcaggttg ccaatatcca cgcgatggcg
    14221 caacggtgta tgaccaaacc agaaatgatc agcaccttta attccctgcc cttttgggcg
    14281 ttcacctaat cgcgagcggc tccacaagac ctgatgcaaa tcaacgtcct tttgccattc
    14341 ataaacatca tctggataat cggcatgagc aataacatgc ttgccggtgc gactgtgtac
    14401 ttcaagaata aagggcaaat gctgacactt ttccagcgca gttttcgctt gtttctgttg
    14461 attatctgcc agtgcaataa accagtcgcc gccattcatc aaccacaaag acatctgctg
    14521 ggatgccagc gcatccatcg ccatctgttc atgattgcct cttaccgcac aaacccaatg
    14581 ttgttccagt aactgcagac aacgtaaact ttgcggccca cgatcgataa cgtctcccac
    14641 tgagataagt aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg
    14701 ctcaagacaa ccatgtatat cgcctgaaag ccagatatgt cgccattgat gacccgcaat
    14761 tctctgataa acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca
    14821 taattttaac aagaatgtta aaaaacgctg gactcagaca gtagagtgtg tgttatggtt
    14881 gactataaag tcagcgaagg aaatgcttct ggcttttaac agataaaaag agaccgaaca
    14941 cgattcctgt attcggtcca gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg
    15001 gcattaatgc aggcttagtt gccttgccct ttaagaatag atgacgacgc caggttttcc
    15061 agtttgcgtg caaaatggtc aataaaaagc gcggtagtca tcagcttaaa tgttaaaaac
    15121 cgcccgttct ggtgaaagaa ctgaggcggt ttttttattg gaaatcaaaa ggctatttta
    15181 ggtaattaac agagtttttc agctcgttct ataaacggtg ccagactcat tttttcgccg
    15241 ggattgttag gatcatcaat ctgaatcacc gaaatcggct gggctttagt cttcccactg
    15301 gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg
    15361 acatacaaag cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat
    15421 ttgtctttac ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct
    15481 gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat
    15541 caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc
    15601 cattcgagtt tagtcatcca cacaaaccag ggtgccgcac gatcttcatc ctgcctcatg
    15661 cgcggtacaa gaacataacg attagccagc gcaatgacca ccataatcat caccaggatg
    15721 gctttgagca ataagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc
    15781 aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag
    15841 gaaaaacgca tcagcgcctg aatggcctgg tgtcgccaac ggcctttgat gagttgcata
    15901 caccagagca ccggcagtaa tcccccaaac cacgccgccg cacaaatcag gtgaatcgcg
    15961 tgattagtct gatggatttt cgccgttacc ccttcattca gtgtcgcatg cccgattccc
    16021 gccagcagaa taaattgcgc ggtagtgagc ataaacagca aacgtggcat attccgcggt
    16081 tgcataaggg cgacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagacgcca
    16141 ccaaactgcg tttgcaaaac agcctgccaa atgtttggcg aaaatacatc tgcccatccc
    16201 gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg
    16261 gcagcgtgct gttgtaaacg cagaaaacgc ttcgccagca agcgacgaat cgttaagggg
    16321 gccagccagg caccgtacat tgcgaagcca aagaccagca tcagcgaggt gaaatggata
    16381 aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt
    16441 cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt
    16501 ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgct
    16561 ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt
    16621 tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac ttgcgcgttt
    16681 gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa
    16741 gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt
    16801 cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca
    16861 tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga
    16921 gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg
    16981 tcgatttggc ggcagccggg gtggcattta aagaacgcta caatatgtcg gtgatcgctg
    17041 aagcggttga acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttattg
    17101 cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa
    17161 acttatacag agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac
    17221 cgcacccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc
    17281 agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa caggtagctc atcatctgga
    17341 gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta gtttttccat cgctttcttt
    17401 actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatctct cactgttaga
    17461 cccgctttgt tatgccgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga
    17521 atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacgcctgg
    17581 gatctaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt
    17641 cgatgaacaa ccgcaaggta tggatatgga cccgacctgt tcactgttta ctacagggca
    17701 atgtctggga gaacccgacc tgctggcgtc ggcccgtcgc ttacaatttt tttcacatca
    17761 gtactcgatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaata
    17821 tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc
    17881 acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc
    17941 atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc
    18001 tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat
    18061 cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat
    18121 aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcat
    18181 aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg
    18241 aaactggccc gtcgtttgtg gcctgggatc aagtacagca atatggcgtt atataaaaca
    18301 cgcaagctca atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt
    18361 tatatcaccg ccgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag
    18421 atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt
    18481 ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttatttaat
    18541 aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact
    18601 tagtccgcag gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc
    18661 atcgctacgc cttcgtacga tttaaagcga ccagatttac cgccatggcc tgagtccatg
    18721 tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat
    18781 tttgccggtt cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc
    18841 ggataagcct gtgcggtgac gttgtcatac gggctgtagc ttttcatgta ctcgtaatat
    18901 tgcggatcct gcgggttacc ccactcttca aactcaccag tggtaagagg aattgattca
    18961 tcaagcatcg ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat
    19021 tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcactccc gcccatcgca
    19081 taacaaagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa
    19141 tcattaaacg tatttttctt cttcagaaat tttccgtctt cgtaccattg ttgccccagc
    19201 tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag
    19261 cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccatagcc atacaccagc
    19321 aacgggttgt gtcctttgcg aaaatgtttg cgatggtaga ccaacgaaac cggaacttcg
    19381 acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaaccg
    19441 ggcacttctg tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa
    19501 gtgtctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga
    19561 ttgtaggcaa tccaggtcac ataggctgga tcatcaaagg caataccaat gacttcccgg
    19621 gtcttgcggt taatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc
    19681 cagtcggtaa acagcgtaaa cccttccagc atgatgtttt cgcgtggcgg aattaactct
    19741 tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt gccgtggcgg
    19801 ttggaacgca gataaaaacg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg
    19861 cgcggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca
    19921 ctggtggtgg cgctggccaa atgaatgact acatagtgct tcgaggtcgt tttatgcagg
    19981 ctgacgtaat aggtatcgtc tttttcttcg tagatcagtt tatcttgcga tgctggcgta
    20041 ccgatggcgt gacgccagac ctgataaggc agcagcgtca ccggatgctt gcgaacatag
    20101 tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc
    20161 gggtaccagt taccagtttc cagattacga aaacgaatgc cgtactggcg tcgggaaaga
    20221 aaatcttctg ccagcgccat aatggtgtta tcgggcgtaa tcgccattcc gcccatcgaa
    20281 taaaactcac gatgagctgc gcgcttattg gcatcgagca atgtttccca ctcatcccac
    20341 tcttcactga atgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata
    20401 tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg
    20461 tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gtgaggccat cacccgatga
    20521 ccgtaactat tttcttgttg caggtagtcc aggacttctg gctgagaacg cgtatcgtcc
    20581 cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc gtggggaatg
    20641 cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc
    20701 aaaactcacc ggggatgcaa gcgaaacagg ggagtcattg cttagatgat gacaggtaat
    20761 ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgttcaaat ggctgtgcga
    20821 ttctggatag cccgaaatag tcaacttcag gctatccaga gagcggaatt attccgccaa
    20881 agtgcgtttt tgctgttcga gatcgcgttc aatgccgtca cgaacatcct gggggatttt
    20941 cagcgcgtca cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc
    21001 cgcgcaactc aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag
    21061 gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc cctgctcttc
    21121 cacgccggct tcacgcaatt gctggtcgat tgccgcacgt tctttggcat caatatgacc
    21181 atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tctgtacgct catccagcgg
    21241 cgtactttgc gcgccaaact gcggttcgtc ctgatgcgcc gcgcgaattt tatctttgta
    21301 tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgttggtgcc
    21361 atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc
    21421 taatgcgcct ggcactaaca gtttgcccaa tccctgatcc gcagacgagg aggtagaaga
    21481 actgctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca
    21541 ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatatcgaga
    21601 caaaagatgc gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta
    21661 aggtatttac tgtatatcag gtgataaggg tattttaggt gaaagtagtt tgtcgttttg
    21721 agtggcattg tttgatgctg attgcccgat gcgacgctgt tgcgtcttct caggcctacg
    21781 attgtggtgc gttctggggc ggacaaggca ctcgcgccgc atccgccaac ggtcaatcga
    21841 attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg
    21901 acctggtaat gaatatcggc acttttacca cgcacggtta gcggtactga ccatttatca
    21961 tctttaccct gaatgtcctg caaactgacc caagccacgg gatcggcctg accgacaatt
    22021 ttttgatcat ctgcccagcg cgccacgcga ttttgttgat aatcacgttt tacgctcgcg
    22081 gcaattccgg cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca
    22141 ttattggcag cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct
    22201 ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt
    22261 caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg
    22321 acgacctgtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
    22381 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt
    22441 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tatcataagc atcggcttcc
    22501 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
    22561 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttttcggtt
    22621 gagaaatcaa catcagcaat aaagacacac gcaaacgttt tcgtttatac tgcgcgcgga
    22681 attaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
    22741 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
    22801 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
    22861 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
    22921 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
    22981 cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
    23041 cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc tgcccacttc cacttatcgt
    23101 tttgccgata gcgaaagcct tttccgcgag gcggttgccg ccattggcta tccctgcatt
    23161 gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt ttattcgttc tgcagagcaa
    23221 cttgctcagg catgggaata cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
    23281 gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat
    23341 ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag atggcgacta ccgtgaatcc
    23401 tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg cgcaggagat tgcccgtaaa
    23461 gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg agctgtttgt ctgtggtgat
    23521 gaggtgattt tcagtgaggt ctcccctcgt ccacatgata ccgggatggt gacgttaatt
    23581 tctcaagatc tctcagagtt tgccctgcat gtacgtgcct tcctcggact tcctgttggc
    23641 gggatccgtc agtatggtcc tgcagcttct gccgttattc tgccacaact gaccagtcag
    23701 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag atttgcagat tcgtttattt
    23761 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
    23821 gttgttgacg ccattgaacg cgcgaagcac gccgccggac aggtaaaagt acagggttaa
    23881 acccggcaaa aaaacgctac aaaaatgccc gatcgtagat cgggcgtttt gacttttaca
    23941 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    24001 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    24061 ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg gcagaaacgg acctgggaga
    24121 acggacccgc gatcgcctgc agggctttca cgccgccgtt agcttcagcc gggaagaatt
    24181 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    24241 gaatcagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
    24301 taattgcgaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
    24361 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    24421 cacactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    24481 ccatcggcac tgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    24541 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    24601 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    24661 cacgcaaggc gctgaataat tcacgtcctg tccccacgcg tgacgcgctc aggtcaggaa
    24721 tgtgcggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    24781 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    24841 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    24901 cggtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    24961 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    25021 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
    25081 ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc agaggttttc ataaccgcac
    25141 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    25201 aagcgatcac attgctgtcg agtgattttt ccgccccttt ccgccagtcc agttcaccat
    25261 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
    25321 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    25381 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    25441 catcagaaag gtcagagaag tcatcccagt taatctgaat accggccgcg cgcgccatcg
    25501 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    25561 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    25621 tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg
    25681 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    25741 tgttggcagt accgtagaaa gtacatgttc ccggcgcgtg ataagacgcg gcttctgact
    25801 ccagtagtgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    25861 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgac
    25921 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
    25981 gagcaccatc aaacatgtta tgggacagcc cgaccgccgc agacatcgct atcacttcgc
    26041 ggcttagcag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    26101 gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg cagggcttta cgaatgattt
    26161 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    26221 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    26281 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacagtcgac gttttcgctt
    26341 gttctatccg ggcgagataa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    26401 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    26461 attcacaacg cgtttcattc agaggattta tgactgaaac gcctgtaacc ggagctcata
    26521 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    26581 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    26641 taaaggataa gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    26701 tcgccaccga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    26761 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcac
    26821 gacgtacaaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    26881 ccgccagatg cgtctgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
    26941 ggttatgttt gtggtcaagg ccaggaactt tattcagtac ctggatatcc acgccttcat
    27001 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
    27061 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
    27121 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    27181 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    27241 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgta
    27301 cgttggagcg gtcgatgcgg cgcagggact tcagtacttt cactttttca tcgcggatgc
    27361 tgtctgcgct caggtcagat ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt
    27421 ggttctgaat catgtcacgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    27481 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
    27541 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    27601 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcattga
    27661 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    27721 tagcattcag ttttgcctcg ccaagccctt tgcaaattgc gccaaaagtg ctaggcggca
    27781 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggttga
    27841 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    27901 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    27961 tatatgccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    28021 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    28081 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    28141 tctccttaag ttaactaacc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    28201 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    28261 cgggaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    28321 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    28381 ttaacacagc acacaggcgt aatatctgac aaaactacat ttcgatttct ttcagtgcgg
    28441 aaatcgtcat tacccgtgag tttctttaca tcatgaatat gctggaaaaa atccagtctc
    28501 agctggaaca tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg
    28561 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    28621 cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc
    28681 tggcacaaag tctggcgaat ggcactccct atgttaatcg caatgtcaat gaagatgaca
    28741 gcgttgaatc atacacaggg aaaatatttg agtccgcaat ggcaacgctt gatcatgtcc
    28801 gtcattcact ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa
    28861 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
    28921 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
    28981 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa
    29041 atctggtcga gctggcgcag ctggctcgcg aaaacgacgc aatggtgatt gccctcacct
    29101 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
    29161 atactgacat ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc
    29221 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    29281 aggaagcgct gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct
    29341 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
    29401 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    29461 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    29521 acagatcgcg ataataatct tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg
    29581 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt
    29641 gccgcaagac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt
    29701 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    29761 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    29821 cctgctgacg ttgtgcctgg cgacatcctg ctgctggacg atggtcgcgt tcagttaaaa
    29881 gtactggaag ttcagggcat gaaagtgttc accgaagtga ccgtcggtgg tcccctctcc
    29941 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    30001 gacaaagcag acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc
    30061 ccacgctgcg gcgaagatct gaactatgcc cgtcgcctgg cacgcgatgc aggatgtgat
    30121 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    30181 gacattattc tcgcctctga cgtggtaatg gttgcacgcg gcgacctcgg tgtggaaatt
    30241 ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac
    30301 cgagcggtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    30361 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg
    30421 tctgcagaaa ctgccgcagg gcagtatccg tcagaaactg ttgcagccat ggcgcgcgtt
    30481 tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    30541 ttcgacaatg tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa
    30601 ggcgttacgg cgattattac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    30661 atcagctctg gtctaccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact
    30721 gctctctatc gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct
    30781 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    30841 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    30901 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc acatccggcc tacagttcaa
    30961 tgatagttca acagatttcg aatattctga agcaaacttg aacttatcat caggcgaagg
    31021 cctctcctcg cgagaggctt ttttatttga tgggataaag atctttgcgc ttatacggct
    31081 ggatttcgcc cggtttgcga gttttcagca attttagtat ccaggtgtat tgttctggtc
    31141 gcggaccaac aaaaatctcg acttcttcat tcatccgccg cgcaatcgta tgatcatccg
    31201 cctctaacag atcatccatc ggtgggcgca cctgaatcgt cagacggtgc gtcttgccat
    31261 cataaatcgg aaacagcggt acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg
    31321 cgggcaacgt cgctttatag gtggcaaaga aatccacaaa ttcgctgtgc tctgggccat
    31381 gatcctgatc gggtaaataa tatccccagt acccctgacg taccgactgg atgaatggtt
    31441 taataccgtc atttctcgca tgcagacgac cgccaaagcg acgacgcacc gtgttccaga
    31501 cataatcaaa aaccgggttg ccctgattat ggaacatcgc tgccattttc tgcccttgcg
    31561 aggccatcag catggcagga atatcgacgg cccaaccgtg cggcaccaga aagataactt
    31621 tctcgttatt acgcctcatc tcttcgatga tctccagccc ttgccagtca acgcgcggct
    31681 gaattttctc cggtccgcgt attgccaact cagccatcat tgccatcgct tgcggcgcgg
    31741 tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc actacgttct ggaaagcaga
    31801 gcgacagatt gattaacgca cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc
    31861 ccagccgtgc cagaatggga tcacggaact ttggcggcgt taaagcgata cccgccatcg
    31921 ctgctacgcc cagccatgct ccccagtagc gcgggtggcg aaaggattta tcaaactcag
    31981 gaatgtattc gctattattt tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa
    32041 aatcaatctg gtgatagtgt agcggcgcaa cttgccccgc accaaataaa aaaagccggt
    32101 actgactgcg taccggctgc gaatggatgt taattaatca aaccgtagct gcggcacaat
    32161 ctctttggcc tgcgccagga attcgcgacg atcggagccg gtcagccctt cggtacgcgg
    32221 cagttttgcc gtcagcgggt ttacggcctg ctggtttatc catacttcat agtgcagatg
    32281 cggcccggtt gaacgtccgg tattaccgga aagcgcgata cggtcgccac gtttcacctt
    32341 ctgtcccggt ttcaccagaa tcttgcgcag gtgcatataa cgcgtggtgt agctgcgacc
    32401 atgacgaata gccacataat aacctgctgc gccactgcgt ttggcaacca ccacttcacc
    32461 gtcacccact gaaagcactg gcgtaccttg tggcatggcg aaatcaacac ctctgtgtgg
    32521 tgcaacgcga cctgtcaccg gattagtacg acgcgggtta aagttagacg agatacggaa
    32581 ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga ccagtaccgt tacgatcgta
    32641 gaatttgcca tcctcagcgc ggatagcgta ataatcttta ccttctgaac gcaaacgtac
    32701 gcccagcagc tggctttgct cacgtttacc atcaagcatt tctcgagaca ttaacaccgc
    32761 aaattcatcg ccttttttca gtttgcggaa atccatttgc cactgcatgg ctttaatcac
    32821 tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg ctggcaacaa agctcccccc
    32881 gacggtacct ttcagcagat tgttaaccca ctctccttgc tgcatttcgc tggtcatttt
    32941 aaaaccgtta gcagcagtac ggtcataggt tcgggtttca cgacgagaca cttcccaggt
    33001 gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag agttgttgac cgattttcag
    33061 attacgcaat tctttgtcgg cagcagccag ttgggtgata tcacccatat caataccata
    33121 ctgattgaga atgctgctta gcgtatcgcc agtggaaaca acatattcat gcacgcccgc
    33181 ttcaccggcg attttgtcat ccagttcgtc ctggggaatg gcttcatctt cttgtgcagc
    33241 ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga atttcgttct gttccagctc
    33301 aatggttttg acaattggcg tggcatcacg gtgataaaca taaggccgcc agacagcgac
    33361 ggccagagta agaacggtga gcgaccccaa cataacgcgg tgtggtcgcg gtaaattatt
    33421 aaacgccagg gcgacagagc gggctatctg ttgcacgtaa tcacttcctc attaatctcc
    33481 tttcaggcag ctcgcatact ggttggctaa ttgactcagg aattctgaat agcttgtttt
    33541 acccagtttg atattcgtcc ccaggggatc caacgttccc atacgaacgg atgtccctcg
    33601 tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca gcaaaaacgc aggttgcttt
    33661 ttgctcaacc aactgtgttc ttatttcatg taaacgctgc gcgccaggtt gaatctcagg
    33721 gttaacggta aaatgaccaa gcggtgtcag tccaaactgt ttttcgaaat agccgtaagc
    33781 atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg agctcgttac caacctgcgt
    33841 ttcggttgag gctaattgtg cctcaaaatc cttcaggttg gcgtcaagtt tggctcgact
    33901 ttgcggcata agttccacta attttccatg gattgcaacc gctgtagccc gcgctatctc
    33961 tggggaaagc caaagatgca tgttgaaatc gccgtgatgg tgatcttcgt cacttttttc
    34021 cgcgtggtcg tgatcatcat catcgccgtg aatacttttc atcagcagcg gtttcacatc
    34081 ttctagctgt gcaatcgtta cctgtttcgc ttctggtaat ttacttaccg gtttttgcat
    34141 aaacgcttcc atctccgggc caacccaaac gactaagtcc gcgttctgta agcgttttac
    34201 atccgatgga cgcagtgaat aatcatgttc tgaagcgccg tcaggcagta aaacctccgt
    34261 ttctgttaca ccatcagcaa tggcagaagc gatgaaccca acgggtttaa gcgaagcgac
    34321 aacggcggca tctgcggcct gtgttgcacc tccccagaga gcggcggata atgctgcgaa
    34381 aagaagcgtt tttttatgta acataatgcg accaatcatc gtaatgaata tgagaagtgt
    34441 gatattataa catttcatga ctactgcaag actaaaatta acatgacaag tctggtttcc
    34501 ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc tctctgatgt gtcgctggaa
    34561 cttaaacctg gaaaaatttt gactttactt gggccaaatg gcgcaggtaa gtcgacactg
    34621 gtacgggtag tgctcgggct ggtaacaccc gatgaagggg ttatcaagcg caacggaaaa
    34681 ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca ccacgttgcc actgaccgta
    34741 aaccgttttt tacgcttacg ccctggtaca cataaagaag atattttgcc tgcactgaaa
    34801 cgtgtccagg ccgggcacct gattaacgca ccgatgcaaa agctctctgg tggcgaaacg
    34861 cagcgtgtac tattagcgcg agcattgtta aatcgcccgc aattattagt gctggatgaa
    34921 cccactcaag gcgtggatgt aaatggccag gtggcgttat atgaccttat tgaccaactg
    34981 cgtcgcgaac tggattgtgg cgttttaatg gtctctcacg atctgcatct ggtaatggca
    35041 aaaaccgatg aagtgctgtg tctgaatcac cacatttgtt gttccggcac accggaagtt
    35101 gtttccctac atccggagtt tatttctatg tttggtcctc gtggtgctga acaactgggt
    35161 atctatcgcc atcatcataa tcatcgtcac gatttacagg gacgaattgt tttgcgtcgg
    35221 ggaaatgatc gctcatgatt gaattattat ttcccggttg gttagccggg atcatgctcg
    35281 cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg tcgtatgtct tatttcggtg
    35341 atacgctggc tcatgcctca ttacttggcg tcgcgtttgg tttgttgctg gacgtgaatc
    35401 cattctatgc ggtaattgcc gttacgctgc tcctggcggg cggtctggta tggctggaga
    35461 agcgtccaca gctggcgatt gacacgttat tagggattat ggcgcacagt gccctgtcgc
    35521 tgggcctggt ggtcgttagt ctgatgtcta acattcgtgt tgatttgatg gcttacctgt
    35581 tcggtgattt gctggcagtg acgccagaag atctcatcgc tattgcgatt ggcgtggtca
    35641 tcgtggtggc tattttgttc tggcaatggc gcaatttgct gtcgatgacg attagcccgg
    35701 atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa attgttgttg atgctggtga
    35761 cggcattgac gattggtgta gcgatgaaat ttgttggcgc gttgattatt acttcactgc
    35821 tgattattcc tgcagctact gcgcgtcgct ttgcccgtac gccggaacag atggctggtg
    35881 tcgctgtttt ggtggggatg gtggcagtaa ctggcggttt aaccttttcc gcgttttacg
    35941 atacgccggc gggtccgtcg gtggtgctat gtgcggcact gttatttatt ctcagtatga
    36001 tgaaaaagca ggccagctaa tctatcgctg aacacatttg tcggatgcgg cgcgagcgcc
    36061 ttatccgacc tacggttcgc tatctctggc tggcctgata agacgcgaac agtgtcgcat
    36121 cagacacact gccagtgtcg gatgcggcgc gagcgaccaa tccgacttac ggcatttctg
    36181 gcggcgttat gccaaagtga ttccacgccc gcgtcgtcgc catacgccca cgcggtgtac
    36241 gctgcaaaaa gccttgctga atcaaataag gttccagcac atcctcaatg gtttcacgtt
    36301 cttcgccaat ggctgccgca aggttatcca gacctaccgg cccaccaaag aacttatcga
    36361 ttaccgccag caataacttg cggtccatat aatcgaaacc ttcagcatcg acattcaaca
    36421 tatccaacgc ctgagcagcg atatctgccg agatggtgcc atcgtgcttc acttcggcga
    36481 aatcacgcac tcgacgcagc agacggttgg caatgcgcgg cgtaccgcga gctcgacgag
    36541 caacttccag cgcgccgtca tcactcatct caagccccat aaagcgtgcg ctgcgactga
    36601 cgatatattg cagatccggc acctgataaa actccagacg ctgcacaata ccaaaacgat
    36661 cgcgcaacgg tgatgtcagc gaacctgcgc gcgtggttgc accaatcagg gtaaacggcg
    36721 gcaaatcaat tttaatggag cgtgccgccg gaccttcacc aatcatgata tccagttggt
    36781 agtcttccat tgccggatac aacacctctt ccaccactgg cgaaagacgg tggatctcat
    36841 caataaacaa cacgtcatgc ggttcaaggt tagtgagcat cgctgccaga tcgcccgcct
    36901 tttccagcac cggaccagaa gtcgtgcgta aattaacgcc catttcattg gcgacaatgt
    36961 tggcaagcgt agttttaccc aaccccggag gaccgaaaat caacagatga tcgagggcat
    37021 cgccgcgcag tttcgctgct ttgatgaaaa tctccatctg tgaacgaacc tgcggctgac
    37081 caacatactc ttccagtaat ttagggcgaa tggcgcgatc tgccacatct tccggcaaag
    37141 tggtaccggc agaaatcaga cggtctgctt caatcatcct ttacctcata acgcggcgcg
    37201 tagggcttcg cgaattaatg tttcactgct ggcgtcaggg cgagcgattt tgctcaccat
    37261 gcggcttgct tcttgtggtt tatagcccag cgccaccagc gcggcaaccg cttcctgttc
    37321 agcatcgtcg gtcgtcgggc tggcaggtga cgtgagtacc aggtcggcgg ctggcgtaaa
    37381 gagatcgcca tgcaaacctt taaatcggtc tttcatttca acaatcaagc gttcggcggt
    37441 ttttttgcct atacccggca gtttcaccag tgcccccact tcttcacgct caacggcatt
    37501 aacgaactgc tgcgctgaca ttccggagag gatcgccagc gccaacttcg ggccgacgcc
    37561 gttggttttg atcaactctt tgaacaatgt gcgctcttgt ttattgttaa aaccgtacag
    37621 cagttgcgcg tcttcacgca ccacaaagtg ggtgaaaacg atcgcttcct gacccgcttc
    37681 agggagttca taaaaacagg tcatcggcat atgcacttca tagcctacgc cgcccacttc
    37741 aattaacacc agcgggggtt gtttttcaat gatgatgcct ctgagtctgc ctatcacatg
    37801 acgctcctgc gtaatgaatc aaagataatg ctgtatgata aaaaaatgct ggatagatat
    37861 ccagcgaagg atgaagaaaa cttgcgaggc gt
//
LOCUS       DAPPNJ010000020        36629 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000020 DAPPNJ010000000
VERSION     DAPPNJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.020"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(384..674)
                     /locus_tag="RPM73_000771"
     CDS             complement(384..674)
                     /locus_tag="RPM73_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX6519163.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(675..986)
                     /locus_tag="RPM73_000772"
     CDS             complement(675..986)
                     /locus_tag="RPM73_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX6519164.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1215..2123
                     /locus_tag="RPM73_000773"
     CDS             1215..2123
                     /locus_tag="RPM73_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX6519165.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2187..3128)
                     /gene="fabY"
                     /locus_tag="RPM73_000774"
                     /gene_synonym="yiiD"
     CDS             complement(2187..3128)
                     /gene="fabY"
                     /locus_tag="RPM73_000774"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDX6519166.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3173..3610)
                     /gene="dtd"
                     /locus_tag="RPM73_000775"
     CDS             complement(3173..3610)
                     /gene="dtd"
                     /locus_tag="RPM73_000775"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDX6519167.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3607..4479)
                     /locus_tag="RPM73_000776"
     CDS             complement(3607..4479)
                     /locus_tag="RPM73_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDX6519168.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4473..5072)
                     /gene="yihX"
                     /locus_tag="RPM73_000777"
     CDS             complement(4473..5072)
                     /gene="yihX"
                     /locus_tag="RPM73_000777"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDX6519169.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5213..6100)
                     /locus_tag="RPM73_000778"
     CDS             complement(5213..6100)
                     /locus_tag="RPM73_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDX6519170.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(6090..7439)
                     /locus_tag="RPM73_000779"
     CDS             complement(6090..7439)
                     /locus_tag="RPM73_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDX6519171.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7538..8971)
                     /locus_tag="RPM73_000780"
     CDS             complement(7538..8971)
                     /locus_tag="RPM73_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519172.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9041..10051)
                     /locus_tag="RPM73_000781"
     CDS             complement(9041..10051)
                     /locus_tag="RPM73_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDX6519173.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10084..10971)
                     /locus_tag="RPM73_000782"
     CDS             complement(10084..10971)
                     /locus_tag="RPM73_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDX6519174.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10996..11874)
                     /gene="yihU"
                     /locus_tag="RPM73_000783"
     CDS             complement(10996..11874)
                     /gene="yihU"
                     /locus_tag="RPM73_000783"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDX6519175.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12047..12943
                     /locus_tag="RPM73_000784"
     CDS             12047..12943
                     /locus_tag="RPM73_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX6519176.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12983..13771
                     /locus_tag="RPM73_000785"
     CDS             12983..13771
                     /locus_tag="RPM73_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX6519177.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13882..15147)
                     /locus_tag="RPM73_000786"
     CDS             complement(13882..15147)
                     /locus_tag="RPM73_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519178.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15249..16229)
                     /locus_tag="RPM73_000787"
     CDS             complement(15249..16229)
                     /locus_tag="RPM73_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDX6519179.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(16237..16947)
                     /locus_tag="RPM73_000788"
     CDS             complement(16237..16947)
                     /locus_tag="RPM73_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDX6519180.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17164..18987)
                     /gene="typA"
                     /locus_tag="RPM73_000789"
     CDS             complement(17164..18987)
                     /gene="typA"
                     /locus_tag="RPM73_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDX6519181.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19360..20769
                     /gene="glnA"
                     /locus_tag="RPM73_000790"
     CDS             19360..20769
                     /gene="glnA"
                     /locus_tag="RPM73_000790"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDX6519182.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20943..21992
                     /gene="glnL"
                     /locus_tag="RPM73_000791"
     CDS             20943..21992
                     /gene="glnL"
                     /locus_tag="RPM73_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDX6519183.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22004..23413
                     /gene="glnG"
                     /locus_tag="RPM73_000792"
     CDS             22004..23413
                     /gene="glnG"
                     /locus_tag="RPM73_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDX6519184.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23525..23635
                     /locus_tag="RPM73_000793"
     CDS             23525..23635
                     /locus_tag="RPM73_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDX6519185.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23825..25198)
                     /gene="hemN"
                     /locus_tag="RPM73_000794"
     CDS             complement(23825..25198)
                     /gene="hemN"
                     /locus_tag="RPM73_000794"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDX6519186.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKVIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25387..25896)
                     /gene="yihI"
                     /locus_tag="RPM73_000795"
     CDS             complement(25387..25896)
                     /gene="yihI"
                     /locus_tag="RPM73_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDX6519187.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            26478..27110
                     /locus_tag="RPM73_000796"
     CDS             26478..27110
                     /locus_tag="RPM73_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDX6519188.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27492..30278)
                     /gene="polA"
                     /locus_tag="RPM73_000797"
     CDS             complement(27492..30278)
                     /gene="polA"
                     /locus_tag="RPM73_000797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDX6519189.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30642..31574
                     /locus_tag="RPM73_000798"
     CDS             30642..31574
                     /locus_tag="RPM73_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDX6519190.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31615..33045)
                     /locus_tag="RPM73_000799"
     CDS             complement(31615..33045)
                     /locus_tag="RPM73_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDX6519191.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(33200..33826)
                     /gene="dsbA"
                     /locus_tag="RPM73_000800"
     CDS             complement(33200..33826)
                     /gene="dsbA"
                     /locus_tag="RPM73_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDX6519192.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(33843..34829)
                     /gene="srkA"
                     /locus_tag="RPM73_000801"
     CDS             complement(33843..34829)
                     /gene="srkA"
                     /locus_tag="RPM73_000801"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDX6519193.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(34906..35175)
                     /locus_tag="RPM73_000802"
     CDS             complement(34906..35175)
                     /locus_tag="RPM73_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDX6519194.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            35245..35829
                     /gene="mobA"
                     /locus_tag="RPM73_000803"
     CDS             35245..35829
                     /gene="mobA"
                     /locus_tag="RPM73_000803"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDX6519195.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            35811..36338
                     /gene="mobB"
                     /locus_tag="RPM73_000804"
     CDS             35811..36338
                     /gene="mobB"
                     /locus_tag="RPM73_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDX6519196.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(36443..36558)
                     /gene="rrf"
                     /locus_tag="RPM73_000805"
     rRNA            complement(36443..36558)
                     /gene="rrf"
                     /locus_tag="RPM73_000805"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9253 a         9362 c         8750 g         9264 t
ORIGIN      
        1 ttttttgatt ggatctttat ttgcataatc tgaatggtgt acccgaaaaa tgtcagcatc
       61 ctaacagcac ccggatttaa ggtgaaatta atcatccatc acagaccggt cagtactgcc
      121 accaaccggt ctttacttgt tcatcgttat aattttgttg gtaacagttc gggaaacaag
      181 gaattccttc cggaaccccc cggtatcatc cgatagcatt gacgtcaatc atcagcgcga
      241 cgatgagttt ttttaatcgc atcatagggt ggcgacgttc agcacgatta cgggtcatag
      301 caaaactcct gggcaggcga ccactatgcc gctttcacag gagttctgaa tgctagtgct
      361 ttctgtatgg cataaccatg cctctaacgg ttaagtgctt gcagcacatg ttgcggatca
      421 ttggcaatcg ctcgcagcaa agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc
      481 cagttacgca acgtgtctac gcttacagaa ataagatcgg caaatttaga ttgtgacaac
      541 cctgaagcgc ggcgtatttc tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc
      601 tgtcgcgttc cttcaataat ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa
      661 agtgccttat ccatttacca cctcgtattt aacaaccgga gcattgcttt ttcctgggga
      721 gacagatcat ctttaatacc cttacgataa ataagcagta atctgatctc ataatgttta
      781 acctgatgaa agtaaataac cctgacacca gcacgtttcc cctttcctcc tgaaacccat
      841 cgaacctttc ttaacccacc agtttcaggt attacctcac catagtcagg attcaaggcc
      901 aggaaaaact gaaaacgact gaattcatcg tcggtcagca gtttttgaac atcttcagta
      961 aaaatttccg tttcgatgaa gagcataatt atacgccatt ggcttagagt aacaatcctt
     1021 tttgtacgtc attgacgtac ccttgaaacc actagcttac tgatagtgct ccagcaaaac
     1081 cacgtctctg ccgggtttgt ctttagattt gcgaggcgct ttccagattt ttcacgcccg
     1141 tgaatttccc tgtatccatc cagcaaaaac gggtgtataa gactgtacat tcatacagca
     1201 aaataagttc tactatggca ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg
     1261 ctgcgggtcg cccctcatcg cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg
     1321 ccagcgctgt ccttgcgggt gagacagaaa cgcgggtaga tattcagact atcgaacttg
     1381 aagggatgac cttacgaata gtgtcgccgc tgaatgcccc gacattactc ccgacgataa
     1441 tttattacta cggtggctgt tttgttagcg gcgggttcgc aactcacgac aaccagctgc
     1501 gccagttagc ctattacggg caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg
     1561 aacatacttt ccccgccgcc catgatgatg cgcaacgagg tgcagaaata gtctggcaac
     1621 acgccgaacg actcggcgtt gataaacagc ggataactct tgccggtgac agtgcgggcg
     1681 gacatctggc gctggtaact gctttgcgat taaagcgtgc tggcgagtgg caaccggcac
     1741 aactcatctt aatttatcct atgcttgatg cgactgcgca tttcgaaagc tacatacgca
     1801 atggtcacga ttacatcatc acacgcgaca ctttactgag cggctttgag atgtatctgc
     1861 caggcattga acgcaggcat cctgaagcca gccctctttg gcgcgatgat ttcaatggct
     1921 tgccacctgt acatatcatt accgctgaat acgacccact ttgcgatgaa ggtgaagcgt
     1981 tgtatcaccg catgacaggg caaggagtgc aatgcacctg ccaacgctac cttggcgtta
     2041 tccacggatt ttttcagtta ggtggtatca gcgaggccgc gcgatcggca ctacgggatg
     2101 ttgcctggag agcgggaagg taacttcgga cctgggccct gaaaaggttt tcagattgaa
     2161 aaccttttca gtcaagtaaa acgcccctac tcttcttcgt tcccgccctc ttcatacggg
     2221 ccaaatggct tcgcgggcag aacgatatac gtgccttcaa acactgcacc cggcgtctcg
     2281 tcgccaaaga tttcaacttg catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg
     2341 agatcgccgc ttaaggcacc aaggtcggct accgcatgag gtttaccgct aatcggcttg
     2401 ctgtagcgga tatgcgcatc cgccagaata atcgttccgc cgagatggcg ttcgcgcagc
     2461 atcagccaga taagccccca accggtgagc gttgccagtg agaataaact cccggcaaac
     2521 agcgtatggt gcggattctg attaccggtt tccggcatgg tggtgataaa tttttgcccg
     2581 gtatattgtt gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc
     2641 tgttgcagct gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcaacgggt
     2701 ttaatcatca aaaaatggcg aatcggcgtg gtggttggcg tggtgatttc tccctgatta
     2761 acaaacccca gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc
     2821 ttaacgcctt cctgacgcgc caccgactcc agggtcatcg ccatcagcgt tcctaaccct
     2881 ttgtcctgca cgtccgggtg aacggccata aagcgaatgg acgcttcatt gtcggcatta
     2941 atatacagtc ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc
     3001 gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac
     3061 atttcccagc gaaactgata gtaacgctct aattcttctt ctgtttgtgg aacccgaagg
     3121 tgatacatag cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct
     3181 gcaaccagaa tgtcacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc
     3241 gtcctgtttg cgtgttcatc tcttgctggc ggcagcgttc gacgaaatag tcatataacg
     3301 cctctgcgcg atccggtgat gcgcctttgg agaaacttgg gcgcatccct cgttcggtat
     3361 ctgcggcgag ggtaaactgg gaaaccacca gtacactgcc gcccgcctgt tgcacgttga
     3421 gattcatctt gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac
     3481 ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc
     3541 caatttcgcc cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta
     3601 atgcaatcat ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc
     3661 cccgagagtg acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca
     3721 aacaaagaga atggggatca ccgccagcac accgtaaatg agctgatatg acgggaacat
     3781 ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac aggagcgcgg cgacaaacgc
     3841 gccgacaatc gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca
     3901 gaaggagatc cacgacaaca gcagcggaaa aatacgcagc acgttgtcga tgacagtatt
     3961 gagatcgctc gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc
     4021 cagcagcggc cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg
     4081 cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcagcaataa
     4141 cgcggtgacg atcagcccgc acgccccaac ggcggtcatc ttgttggaat tggcaacaaa
     4201 ttgttcgata taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa
     4261 gtgacgtaac tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac
     4321 aacggcaacc agcggcacta atgagagcaa cgacacatag gcaaggttac ctgccagggt
     4381 tgtcatgttg tcctcatcaa tgcgttgcca gagtagtttt agccaggccc atagtggacg
     4441 ggtacggtgc ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc
     4501 gggatggtgg ttttatcttt caccagaata ctggtaatgc ccagctgatt ggctccttct
     4561 atattatcgg cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc
     4621 aaaacatgct ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag
     4681 atatggtcag cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg
     4741 cggttggtat tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg
     4801 gcgatcactt ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg
     4861 tagcttagcg gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt
     4921 tccccacgct catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc
     4981 agcggaatac gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca
     5041 atcacattac ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat
     5101 taactgtagc gggaaaggat gggtttggct atgtggcaat aagggtaatc ttgtgatgct
     5161 gattgcgaaa aagcagagta atttcactgg ctttttcgca atagatgaat ggttaagatt
     5221 caggtgtaaa ttgccaacga gtttcgatga ccagcggcct atctggtgtc accaattccg
     5281 gcgttagatt tattgcgtta ggtgcctgag tcagcgggtt aatacaggtg gcatcgggct
     5341 gtttatcaaa taccaccaga ctgtttgcag aagagatcat ggtcatcgcg agtttaccag
     5401 gccacagtaa cttaaccttc accccgtcat aaaaaccaaa acagtcatcc cagggaccgg
     5461 cttgaggaga gatacgattt ccagttggca gttcatcact acccgcttct tcttgccagt
     5521 ccgcatcaaa aagcacctgc aacgattctg tattttgcgg agtcagcttt ttcgcaaacc
     5581 acggatgcca gcctgcggag gccgggaatg tatcggcata tgaatggact tccagttgca
     5641 aaaccagcgc atcgttctcc agcaaaaaag tctgaatcac ttccccttgc cagggccacg
     5701 gtgaagccag gggcatacgt aaagtcaggg aatctgctgt tttgtcgatg atttcccacg
     5761 ttgagtaaca agccatacca tgcaacgcat gtggtggttt attggcgggt aaagaataac
     5821 attgcccacc agcattcagc gtcgcattcc ccagtcgccc tgcccacggc accattggga
     5881 aacagccata ctgaaaagct ctgcgctgcg gctgccattg gcgtaatact tcagaaccaa
     5941 atgcctttaa cgaggttatc ctggcaccat catgcggata aatctgcagg tgaatattgc
     6001 ctttttccag cgtcactaac tctcctacag gcccaggcca tggcgcgtca aacgcctgtt
     6061 gtagcgccag gtaatgctcg ttttttcgtt cagttttcat gagattgttc cctgatgtag
     6121 cggtaagaag cggtttcttg ttcatcggtc ggctcaatca gccagacgca aacaaaggaa
     6181 agaactgaga cagcaaataa aaacagagcg acataccacg gttttcctcc ccctagcgcc
     6241 agcaaacttg aggcgataag ggggcttagt ccgccaagaa tcgcggaaaa ttgataagca
     6301 aaagaaaggc cggtatatcg tactttggta ccgaacatgc gggtgaaaag tgttggctgc
     6361 acggcaaaca tcatcgtcgg acctaagttg tagccgagga tcatactgca ccagatgatg
     6421 agcgtacttt tgctatccag taataagaag aaaggaaacg cgaacaggac acaaaatcct
     6481 gctcccgaca aatacagact tttttgacct atacgatctg ataaccatcc aaccaatgga
     6541 caactgaaaa taccgatcgc cgaggcaatc aacatacccg tcaggggaat atctcgcgac
     6601 aatgctaatt ggctggaaat atagacaata ccaaaggcgt taataatatt agaggagaca
     6661 ctttccgcca gcctcgcacc cagtgccaat aaaatatttc gtggatgttt tttgaataat
     6721 tcaatcaacg gaggaacgga cttttcttta ttattcgtcg ttttttgctt ttgaaaatcc
     6781 agcgtttctt cggtatgcag gcggataaac agcccaacga tcaacatcac cgcagaaagc
     6841 caaaacggaa tgcgccatcc ccaggagaga aactgctccg aagtaagaaa atggttacag
     6901 agagcaaaaa taccggttgc cagcataata cccacagacg cagccgtttg cgggagtgaa
     6961 cctaaaaatc cgcgtcggga ttcgggcgct gattctatgg tcatcaatgc cgctccgcca
     7021 tactcgccac caagaccaaa gccctgaatt aaacgcagcg ccattaatat gagcggtgcc
     7081 cagatcccaa tttcctgata cgtcggaata aaaccaatca aaaatgtgga gcaaccgaca
     7141 atagccagtg tccagataag tgttattttt cgcccaattt tatcgccaaa atggccaaag
     7201 acgataccac ctaaaggacg ggtcaaaaaa ccaacgccaa aggtagcaaa ggcggctata
     7261 agacctataa aaggatcgct gtctggataa aacagcggtg caaataccag tcctgctgcc
     7321 gtgccaaaaa taaagaagtc ataccattcc attaacgcgc ctgcgaaact gcccataacg
     7381 acacgtctca tggctttacc ggaaagccct tcacgcggtg cgggtaactt ttcagacata
     7441 attttcctct cctggtaggc gagaaatctg ggatacgaac cactaaacgt gggcagtttg
     7501 aattaatgcg catcggttta agccgatgcg cattaattta tttcttcaat tcagccagaa
     7561 cctctgcatc ctcagcatca acatttgcac ccagtggttc ccaattaatc aatacgcagg
     7621 tcaggaaccc caagaatggc acgattgcca accagtaaaa cgcttcagtt ttcaacgccc
     7681 cccacagcat cggccagaaa atgagtcctg caattgcccc tgtgcgcata atagcgcggg
     7741 caaatccgac cccagtaggg cgaatagcgg gaggataaga gagtgtggca atggtcatgc
     7801 ccagcccacc aggacccgct gaatggaaga aaataattga tcccaataac cctaaagaca
     7861 gccatggatt agtcgttgcc agtgcgccta acgacagcaa tgctaaggtc acaacagcaa
     7921 atccatacat cgactgtcgg cgagtaccca gtcgttggag aataaacgag ccgatcatcc
     7981 cgcctgtcac cccacaaagg gcattcacga cggcagaacc cgtcaacgca ccagtaagcc
     8041 cgccacttat tattcccgcc aacgtaagag gaagataaac acctacggcg ttatattgcc
     8101 acgcctgcat ggttgccacg acacagccaa gaatggtgcg tttgcggtaa cgatcattaa
     8161 ataagatgcc atatccacca ctgtatttat tctctgcttt ttccgagaat ttcgcttctg
     8221 ttgtactcga tgccgcaacg cgagcctgaa caccataacg ttttcccaga atgtcgcacg
     8281 cttcctggta acgcccgacg cgtgcggccc acattgcgga ttcaccaata aagaagtaac
     8341 gcaaaatcat gaagatgact gcaaaaatag ctccgatgaa tattccgtaa cgccacaatt
     8401 gttcttcagc gatacccgac agaagcagcg gcaagagcac caaataaaca acagttgtcg
     8461 aaacatacca ggccatttgc cagaggttga ccgacgttcc ggttttcttc gacgttgaac
     8521 cacgaagctc tgccacggca ttggttgcca aagggaagtc gatgcctaaa ccaaagccca
     8581 tgatgacgcg gcacaccagc acccaccaga tactgggcgc aatagctacc gcagcagccc
     8641 cgatggtgca cagccccatg ccaataataa atgctcgctt ttgcccaaag cggtttatta
     8701 ctcggttcgc taataaaccg ccaattaacg cggcgattaa tactgacgca ttaacggttg
     8761 cggcaaggcc tggagaaatg ccaaattgag cggcaatgta tttattacca aaccccacca
     8821 tcgccgcctg gtaggcatca atcaatatgc cgcccaaagc aataaaaata acgccgatac
     8881 tggtgttgat tgcagagttc ttattaacaa tatcaattac gtcctgtggc cgactaattg
     8941 aataagttgc aggactggtg atttgactca tcgcagtgtc ctttttaatt gttgtagggt
     9001 ggcgctgacg tgacgcagcg ccagacagag atttagaaaa tcattccggc gcaaattgca
     9061 gcatgacctt tatggagtca ttttgctgcg cgcgttcgaa tgccgccgct gcatcctcaa
     9121 tggcaaataa atcggtaacc agttcactgg tatcaaaccg cccatccgcc agccattcga
     9181 taacagcatc gaaatcttca gggatgtaca tgccagagtt gagcaagtca cgctcgaaac
     9241 gttgcatcat cggtaatggt atttcacgcg gcccacttgg tacgcccatg cacacgacgg
     9301 tgcctccggc atatacacgg gtacatgcat cgttaagcgt ggcctgcgct gcaacgacat
     9361 caatcgcccc cgtaaatcgc acatctgcgg ccagctcacc tggtgcccaa acttctgctg
     9421 cgcccagctt taacgccagc gcgcgtttgg cagcgtcagg ttcaatcacg gtaatcttac
     9481 ctgcccccat aatgcgcagt gcctgcacga tggataagcc tatcgttccg gcaccaatca
     9541 ccagtacgct ttccagagaa gctttcggca ttcggttaac gcagtgacgg gcacaagccg
     9601 ccggttcggc aaaggccaac acttttagcg gtaaagaggc tggtgcgaca tggcaattac
     9661 gcgcaggaac aatgtgttgt gagcgagcaa agcccggtgc gcgaaaacca gcaacctgcg
     9721 gtggttcaca aagattaaaa cgtcctgctt tgcaggctcg gcattccatg caagccatga
     9781 tgggatcgac cacaacatga tcgcccggct gtacattttt aacgtcgctg ccaacttccg
     9841 taacgcgcgc tgcaatttca tgaccgggga cgacaggtgg tttagcaaac ggatgaccgc
     9901 ctttcagaac atgcagatcc gaaccgcata tcccatagaa cacaggcgca atccgcactt
     9961 cgtgcggttt aagttcctgg tgtggtactt caacatattg agtggtgact ttcccaatat
    10021 ctgaaaataa aacctgggta attttatcca ttgttattct ccagaatcga tatccatagg
    10081 ggattagcgt tgcgcgatac cttcatcgac aatttctgcc aaccgttgta aacgaggaac
    10141 agagacatcg cgcagcatag tttgtggatc ttgtgcacca acaaccgacg cccaaaccgc
    10201 tcgtccagcc aggaagcctg acgcaccacc tttcatggca atacttactg cacggccaaa
    10261 aatatcggca tcaacgccag aagaaagaat tacccacggc atcttcatct gatcgttaag
    10321 ttgctgacag gcggcaagta aggttttttc atcgcctttg ccccctaatg gcatctcggc
    10381 tttgtaaaga tcagcctctg ttccaccaag ctctgcggcg gcggcaacaa tggcgctttc
    10441 acgatcaaag tcccaaccgc gacgaggtgg acgcaccaca ggttcaataa tgctaaccag
    10501 cccggcacta cggcagcgac gaacaaattt atcaaccatt gccagacgtt cctctgcggg
    10561 ttcatcttca cgccagagta caagtaattt cattgcctta gcccccattt cacgggcttt
    10621 atgcgggtcg acggacatat ctatttcaac gctatcaacc gggatgccat tacctgggat
    10681 aaaaagatcg gcagcgacaa tcaggccgca agaattagca acagcgccct gctcgactat
    10741 ttgctcaaga caaaattgtt tatccgccag aacggcagaa gcataaggag aaaggattcg
    10801 ggtcgcggca actttaaaat cggtgagtac actgtcggcg atgggttttg gctgccctgc
    10861 tgccgcaaac atcaagcgca tggcctcacg ctgatcgaca gctaacatgg caaatccacc
    10921 acctgggcgg gagatatctt tcagcgtaaa tggggtgaag tgggatgaca tagaaactcc
    10981 tgttaataga taaaattaaa gaccagcctg ctggcgaacc tgatttagca gggcactcca
    11041 gtcctgccgc cctttgccgt cttgacttgc tgcagcgtaa taactttctg ctgcaatccc
    11101 catattgagc ggtgcccccg tctcccgagc gacgtcaact gcaatacgca gatctttcaa
    11161 cgccagatcc accatgaagg cgggtgaaag atcgcctttc agcactttgc aaggccaggt
    11221 ggtcgtaaaa tgtcctttcc ctgctgccgt accactcatc accttaattg cgacatcaag
    11281 atttaatccc agtgactcac ataacacagc ggcctcggag gacagcgcat taagcgcaat
    11341 gctcatatag ttgttaatta gcttcacgcg gatccccatt cctgggccgc cagcctcgac
    11401 cagctcatta cccatgcaca tgagaatcgg gcgtgcacgt gcaatctgct catcagtgcc
    11461 acccgctaaa atcagcaatg tgccatcaat cgcattcacg gacgttctgc ccacgggggc
    11521 gtccatcata ctgatgcctt tttcttgtaa ctcccgtatt aatgcatcgg tctgtaaggg
    11581 atgaatagtc gacatatcaa tcaataacgc gtcagatgaa acagcctcac agacgccttt
    11641 ttcccccaac agaacctgtc gaacaatgtc accattggga agcatagtga tgataaattc
    11701 agcgcctgct gccgcttccg ctggggtttg tgccgcagtg gccccctgcg taactagcgc
    11761 atcgacggcc tgtgcattga catcaaatac ctgcaatgaa tgcccttttt gcaatagatt
    11821 gttagccatt gggctaccca tctgccccag cccgagaaat gcaatggttg ccatgaatac
    11881 tctcctgtct gacattgaca aaataatgac aattgaaaat tcgttttcgt ctgtttttga
    11941 gcatatttgt aatttatgtt caaaaacacg acacgcatca ctaatttgat catttgtgtc
    12001 atttaaattg accgaagacg acaaaattaa acaggacaag acaaatatga ccaaaattgc
    12061 atgcgtgggt atcacagttc aggacaggat ttattctttg ctcactctgc cggatggcgg
    12121 cggaaaatat caggcaaatc attatcttga gattggtggc ggcccggcag caacagccgc
    12181 tgttgcaatc gcaaagcttg gcgtagaggt tgatttcatc ggtcgcgtcg gcgatgacag
    12241 ttgtggaaat accttacttg cagaacttga gggttggggc gtcaataccg cattttgccg
    12301 ccgatatcct aacgcccgtt catcgcaatc agcgatcctc gttgatcagc atggcgaacg
    12361 gattatcgtt aattatccaa gtcccgatct cggtactgat gccgaatggt tagaagcgat
    12421 cgatttctct cgttatgatc tgattctggc cgatgttcgc tggcatagcg gtactgaaaa
    12481 agcattctcg ctagcgcgtc ttgcgggtgt gacaacgctt ttagacgccg atatgacacc
    12541 gcaggatatt tctccacttg tcgcgcttgc cgatcacgcc gtgttctcaa caccagggct
    12601 gaaacgcatg actggcctgc agtcacctga agaaggtttg ttccaggcaa cgacacaaac
    12661 agcaggtaaa gtgtatgtta cgctgggttc tgaaggatcg ttgtggattg aagatggtca
    12721 tttgtgtcaa caggaggcat tttctgtcaa tgtggttgat accactggag caggcgatgt
    12781 ttttcacggc gcactcgcag tagcgttggc agaaaaaatg ccaacaaaag aggctattcg
    12841 ttttgccagt gcggttgccg cgatgaaatg tacccagcct ggcggtcggg ccggcattcc
    12901 taatcgtgag caaaccgaat catttttgtc actttatgcg taaaatggta aacatctacc
    12961 gaggaggggc aagggagtat tcatgagtat catcgaagtg acaggtaatc cacgccatga
    13021 ccaactcgtt catctaatcg cagaacgcgg ttatatgaat attgaagaac tggcacaact
    13081 gctggatgtt tcaacgcaaa ccgtgcgccg ggatatccga aaactcagtg agcagggctt
    13141 gatcacccgt catcatggag gggccgggcg cgtctccagc gtcatgaata ccgcttttga
    13201 gcaacgggaa ctttcgctca ccgccgagaa acgggcgatc gctgaggcag tcgccgatta
    13261 ccttcccgaa cgctgtaccg tctttatcac catcggtaca accgtagaag ccgttgccag
    13321 ggcattactc aaccggcgtg atttacgcat tatcactaac agcctacgtg tggcacagat
    13381 tctttataag aatcaggata ttgaagtgat ggtgccggga ggcactttac gcgctcataa
    13441 cggcgggatt atcggcccag gagccgtgga ttttattgaa ggcttccgcg cagattattt
    13501 aatcaccagt ataggtgcca ttgaacatga cggcacccta ctggaatttg atgtaaatga
    13561 agcgttagtc gcgagaacga tgattaaaca tgcgcggaat acattgttag tggccgatca
    13621 tacgaagttt gccgcgtctg ctgccgtttc aattggcaat gcacggaatg tcagggcttt
    13681 ttttacagat gccccgcctc ccaattcttt ctgccagttg ttaagtgaag agaatgttga
    13741 actggtggtt gccgagcagg aagtttcctg aaattgaaac ttcgaaaaat ccgctgggga
    13801 ttcagcggat tttattttgc cggatgcggc ctacggttga tacggttttc gttggccgga
    13861 ttaagcacat ccggccatta attaagcttc agccgccagc atctctttct tacgttggct
    13921 aatacgctta accagcatta cggaaaccac cgcaccacag aatgcgaagc aggccataat
    13981 gccaaacacg attttgtagc caataatccc cggatttaaa tccagaatgt agccatacag
    14041 actgaagcag aacatcgccg gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc
    14101 tgttttattt tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt
    14161 aaagactatc gcgccaaagc ccagcgtaca tgccatccct aaatagaccg gcatactttc
    14221 gtgcggcagc ataatcaaca gtacgagcgc agcggtactg atgataaagg tgtagcatag
    14281 atatttactc ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat
    14341 cattttcaga cagtattggt taatgatgcc gtaagccccc accagcgcaa cgggcaatag
    14401 atagatgttt ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa
    14461 gaagacgtta aaagcgataa gccagatggt tttgtctctc agcaccgagg tcatactggt
    14521 gttttgcgat gctccgtctt cttttttcac ctcaacggac ggtgcctctt ctttgtcatt
    14581 caggacaaag aaaataataa tgcctacggc aatcacaata agggaataga accagatgcc
    14641 tgctttaaaa cccaataagc cactgccaaa ccaggtaaat actgccaacg cagaaaatgc
    14701 caccacggta tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg
    14761 ttcgctattg cccaatcgac ttaccgactt gagcaagacc ggccagttca tcatgtcgca
    14821 agtaacgcca aatagcgccc agacaaagag gatgccccaa taccccggca ttgtcgtcaa
    14881 ataaacaccc agtaatcctg tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag
    14941 tttatcggca aaataaatag acagaaaaaa acccactgtc gtgacaaatg agtttaccga
    15001 catagcatta ccaatttgac cattggtcaa atggaaatat tcctgcatcg ggatataaaa
    15061 cgcatctttc agcgacggta atttataaat tgtaccgcca cacaatgtta atagactaaa
    15121 taacgcccat ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt
    15181 ttaaaggcaa taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt
    15241 aaaacttatt agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta
    15301 cgcacatgat ttatctgatt aattgcatct tctttttctt tacgtaaccg cgcatccagt
    15361 aaatgatttt ctggcaatgg tgtttcactc atctggcgat aagggatcca cggattatca
    15421 tcttcgtctt caaagcggaa cgccggcgca tagtaatcaa cctcttcttc caggccatat
    15481 gccgtcacct gcggctcatc atcacccagg catataaggt gcgttaataa cgctttgaac
    15541 ggcatatcac cctgacctga aatacaggct ttatgaccca ggccacccgg ttctttaacg
    15601 atcaaggcat ctttgatatg gacctgggta atatgcggcg ccatcgtttt taaagcgtcg
    15661 atgggatgct catttgcatt gatcatgttc gcaaaatcaa acagtaagga aagagattcc
    15721 atctcacttt ctttgaccag cgacaccagc tcatggcttt ttaaatcttc atgctgttcg
    15781 atcgtaaaag tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg
    15841 atgatcgata atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgat
    15901 gccccagttt tcaacgcaat ggcgacggct tcgtcaatag atgccttatc tgaggcgctg
    15961 gtttcaatat gaatatcaag gttcagacgg cgggctttat ctccaaaggc agagagttct
    16021 ttatcgtcca tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca
    16081 cgtagattat tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc
    16141 atatttaaat gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga
    16201 atctttcttg cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac
    16261 ggcctcaaat cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg
    16321 atgcttttca attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctgatatc
    16381 gcccggcatt ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca
    16441 cttttcccag actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac
    16501 ttcaggatta atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc
    16561 ggtgatgcga tagagttcag ttgatggtgt cactgcaatc agtggcgcga gcgtctcggg
    16621 aatatcgctg gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat
    16681 tgcggcccgc tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac
    16741 gaaccacccc ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc
    16801 ctggcgcagc gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag
    16861 ttttccaccc ggcgtggtga tgccatcttt taaccaccga tccagtttct cttttgcatt
    16921 ttctacggtg gattgatttc ccgtcattgt ttaatccttt tggtttaaac cagatgaggc
    16981 atcttacgct tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc agataattgt
    17041 gacaaagctc tctattgacg taatcccaga cttataaggg agatactcgc attggtgtgc
    17101 gctcacgaca ggtaaaaaaa aacctgccag cgatggcagg ttttttataa ctaaaaaacg
    17161 aaattaatcg tctttcggtg cgcggttggc gcggcgacga tcgttttccg tcaggtgacg
    17221 tttacgaata cggatagagg tcggagtcac ttctaccagt tcgtcatcat cgatgaactc
    17281 cagagcttgt tccagagtca tgcggatagg cggaaccaga acaacggctt cgtcagtacc
    17341 ggaagcacgc atgttggtca gtttcttacc ggtcaggcag tttacagtca ggtcgttaga
    17401 gcggctatga ataccgataa tctgaccttc gtaaacttct gcaccgtgac cgaggaacag
    17461 cttaccgcga tcctgcagac cgaacagcgc gaacgcgacc gctttaccct gaccgttaga
    17521 gatcagtacg ccgttctgac gctgacccac ttcacccgga cgtacgtcgt cgtagtggct
    17581 gaaggtggag tacagcagac cagtaccgga agtcatggtc atgaactcag aacggaagcc
    17641 aatcagacca cggcttggga tcacgtagtc gagacgtacg cggcctttac cgtctggatt
    17701 catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc attacagaac cctgatgctg
    17761 ttcttcaacg tccagagtca cgttttcata tggctcttgt ttacgaccgt cgatttcacg
    17821 gaagataact ttcggacggg ataccgccag ttcgaaacct tcacgacgca tgttttcgat
    17881 cagaacagac aggtgtagtt cgccacgacc agaaacgcgg aacgcatcag cgtcttcggt
    17941 ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat ccaggatctg
    18001 acgagacgtt acgaacttac cttctttacc gcagaacggc gaggtgttaa cgcagaagaa
    18061 catagaaacg gtcggctcat caacggagag tgccggcagc gcttcaacgt tttgcgtgtc
    18121 gcaaacggtg tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc
    18181 ttccgccagg tcggtttcga tacgttccag accgaggtgg cccagcactt taccgacttt
    18241 cgcgttgcgg gttttgcctt cgctatcgat gatagtgacc tgctggttcg gcttcacttt
    18301 accgcgcttg atgcggccaa tgccgataac gccaacatag ctgttgtaat cgagctgaga
    18361 aatctgcatc tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat
    18421 cgcctggtac agcggggtca tgtcttccgc catatcttcg tggtccagac ccgcgatacc
    18481 gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt
    18541 aacgaacaga tcgaatacct gatccacaac ccaatcagga cgcgcgccag ggcggtcaac
    18601 tttgttgata acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg
    18661 cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc agcactgagt ctaccatgga
    18721 cattacacgt tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat
    18781 acggtaatca ttccatttga tagcggtgtt tttcgcgagg atggtaatcc cacgctcttt
    18841 ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc
    18901 ggattgttgg agcagcttgt ctaccagggt ggttttacca tggtctacgt gcgcgatgat
    18961 ggcgatatta cgcaattttt cgatcacaac tttgcctcag gcattagaaa tagcgcgtta
    19021 ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat
    19081 tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg
    19141 caccaacatg gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcgt
    19201 ggtgcagccc ttttgcacga tggtgcgcat gataacgcct tttaggggca atttaaaagt
    19261 tggcacagat ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg
    19321 ttaccacgac gaccatgacc aatccaggag agttaaagta tgtccgctga acacgtactg
    19381 acgatgctga acgagcacga agtgaagttt gttgatttgc gcttcaccga tactaaaggt
    19441 aaagaacagc acgtcactat ccctgctcat caggtgaatg ctgaattctt cgaagaaggc
    19501 aaaatgtttg acggctcctc gattggcggc tggaaaggca ttaacgagtc cgacatggtg
    19561 ctgatgccag acgcatccac cgcagtgatt gacccgttct tcgccgactc caccctgatt
    19621 atccgttgcg acatccttga acctggcacc ctgcaaggct atgaccgtga cccgcgctcc
    19681 attgcgaagc gcgccgaaga ttacctgcgt tccactggca ttgccgacac cgtactgttc
    19741 gggccagaac ctgaattctt cctgttcgat gacatccgtt tcggatcatc tatctccggt
    19801 tcccacgttg ctatcgacga tatcgaaggc gcatggaact cctccaccca atacgaaggt
    19861 ggtaacaaag gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc accggtagac
    19921 tcggctcagg atattcgttc tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt
    19981 gaagcccatc accacgaagt agcgactgct ggtcagaacg aagtggctac ccgcttcaat
    20041 accatgacca aaaaagctga cgaaattcag atctacaaat atgttgtgca caacgtagcg
    20101 caccgcttcg gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga taacggctcc
    20161 ggtatgcact gccacatgtc tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa
    20221 tacgcaggtc tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa
    20281 gcgattaacg ccctggcaaa cccgaccacc aactcttata agcgtctggt cccgggctat
    20341 gaagcaccgg taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg
    20401 gtggtttctt ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac
    20461 ccgtacctgt gctttgctgc cctgctgatg gccggtcttg atggtatcaa gaacaagatc
    20521 catccgggcg aagccatgga caaaaacctg tatgacctgc cgccagaaga agcgaaagag
    20581 atcccacagg ttgcaggctc tctggaagaa gcactgaacg aactggatct ggaccgcgag
    20641 ttcctgaaag ccggtggcgt gttcactgac gaagcaattg atgcgtacat cgctctgcgt
    20701 cgcgaagaag atgaccgcgt gcgtatgact ccgcatccgg tagagtttga gctgtactac
    20761 agcgtctaat agttgaagtt gtactacccg gcgcaacaac gccgggattt agttgccgtg
    20821 gaaactttca gcccatctct gcatgggctt ttttctccgt caattctctg atgcttcgcg
    20881 ctttttatcc gtaaaaagct ataatgcact aaaatggtgc aacctgttca ggagactgct
    20941 ttatggcaac aggcacgcag cccgatgctg ggcagatcct caactcgctg attaacagta
    21001 ttttgttaat tgatgacaat ctggcgatcc attacgccaa ccccgccgcg caacaactgc
    21061 tcgcccaaag ctcccgcaaa ttgtttggta caccgttacc ggaactgttg agctacttct
    21121 cattaaatat cgagctgatg caagaaagtc tggaggcggg gcaaggtttt accgataacg
    21181 aagtgacgct ggtcatcgac gggcgctcgc atatcctttc tgtgacggcc cagcgtatgc
    21241 cggacggcat gatcctgctg gagatggctc cgatggataa ccagcgccgc ttaagtcagg
    21301 aacagctaca gcacgcccag caggttgctg cccgtgattt agtgcgcggc ctggcacatg
    21361 agattaaaaa tccgcttggc ggtttacgtg gcgcggcgca gctactcagc aaagcgttac
    21421 ctgacccatc actactcgaa tataccaaag tgattatcga acaggcggac cggctgcgaa
    21481 atctggtcga ccgtctgttg gggccgcagc tgcccggtac gcgcgttacc gaaagtattc
    21541 acaaagtagc tgaacgcgtg gtaacgctgg tgtcgatgga actgccggac aacgtgcggt
    21601 tgattcgtga ttacgatccc agcctgccgg aactggcgca cgacccggat caaattgaac
    21661 aggtcttgct gaatattgtg cgcaatgcgc tacaggcgct ggggccggag ggtggtgaaa
    21721 tcattctgcg tacccgcacc gcgtttcaac tgaccttaca cggcgagcgc taccggctgg
    21781 cggcgcggat tgatgtggaa gataacgggc cgggcattcc gcctcatttg caggatacgc
    21841 tgttttaccc gatggtcagc ggccgcgaag gtggcaccgg gcttggctta tccatcgctc
    21901 gtaatttgat tgatcagcat tcaggcaaaa ttgaatttac cagttggcca gggcataccg
    21961 agttctcggt ttacctgcct atcaggaaat aaaggtgacg tttatgcaac gagggatagt
    22021 ctgggtagtc gatgacgata gttccatccg ttgggtgctt gaacgtgcgc tcgctggggc
    22081 aggtttaacc tgtacgacgt ttgagaacgg cgcagaagtg ctggaggcgc tggcgagcaa
    22141 aacgccggat gtgctgcttt cagatatccg tatgccggga atggacgggc tggcgctgct
    22201 caagcagatt aaacagcgcc atccgatgct tccggtcatc attatgaccg cacattccga
    22261 tctggatgct gccgtcagcg cctatcaaca aggggcgttc gattatctgc ccaaaccgtt
    22321 tgatatcgac gaagccgtgg cgctggttga gcgcgccatc agtcattacc aggaacagca
    22381 gcagccgcgt aatgttcagc ttaacggccc aacgaccgat atcatcggcg aagcgccagc
    22441 catgcaggac gtgttccgta ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat
    22501 taacggcgaa tccggcaccg gtaaagaact ggtcgctcat gccctgcatc gccacagtcc
    22561 gcgcgccaaa gcgccgttta tcgcgctgaa tatggcagct atcccaaaag atttgatcga
    22621 atcagaactg tttggccacg agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg
    22681 gcgttttgaa caggccgatg gcggtacatt attcctcgac gaaattggtg atatgccgct
    22741 ggatgtgcag acgcgtttgc tgcgcgtgct ggcagacggt cagttttacc gcgttggcgg
    22801 ctatgcgccg gtgaaagtgg atgtgcggat tatcgctgcc actcaccaga atctcgaaca
    22861 gcgagtgcag gaaggtaagt tccgtgagga tctgttccac cgcctgaacg ttatccgcgt
    22921 tcatctgccg ccgctgcgcg aacgtcggga agatattccc cgtctggcgc gccatttttt
    22981 acaggttgcc gcgcgcgaac tgggcgtaga agcgaagtta ctgcatccgg aaaccgaagc
    23041 tgctctgacg cgtctggcgt ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg
    23101 gctaacggtg atggccgccg ggcaggaagt gttgattcag gatttgcccg gcgaactgtt
    23161 tgaatcaacg gttgcggaga gtacttcgca aatgcaaccg gacagctggg cgacgcttct
    23221 tgcgcagtgg gcagacagag cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca
    23281 gccagagctg gagcggacgt tactgacgac cgcgttgcga catacgcagg ggcataaaca
    23341 ggaagcggcg cggctactcg gctggggccg caacaccctg acgcgtaagt taaaagagct
    23401 ggggatggag tgattcacag cttgtgtgta aagattgatt attgagcgca aattgctggt
    23461 attttacgct ttactgttcc gataagttca gtatgatctt gcccggaaaa cggggagagt
    23521 cattatgctg gaatcaataa ttaatctggt atcgagtggc gcagttgaca gccacacacc
    23581 gcaaactgcc gttgctgccg tgctgtgtgc cgcaatgatt gggctgttta gttgagtttc
    23641 agattgctga tgaagtgctc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    23701 tacaaaagca atattttcaa caggttttgt aggcctgata agcgtagcgc atcaggcaat
    23761 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
    23821 ctatttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
    23881 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attaccttct
    23941 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
    24001 agtaatcagc gaagtgcaaa tcccactgtt tctcaatagg ggcgtaatcc agacggaagt
    24061 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
    24121 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
    24181 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
    24241 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
    24301 ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat accgataaac tgatagcccg
    24361 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
    24421 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
    24481 agacactcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
    24541 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
    24601 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
    24661 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
    24721 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
    24781 catcggcatt gaactggaag ttttcgcgca gcagcttcat caggcggctg atttgcgctt
    24841 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
    24901 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
    24961 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
    25021 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
    25081 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
    25141 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    25201 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    25261 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    25321 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    25381 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    25441 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    25501 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    25561 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    25621 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca cgcccaatgg
    25681 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    25741 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    25801 cttgcgatca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    25861 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    25921 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    25981 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    26041 ccgccaacac accctgtttt tcctttagcc ggaacaatcc gtgttgattc catttccgtt
    26101 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    26161 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    26221 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    26281 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    26341 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    26401 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    26461 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    26521 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    26581 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    26641 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    26701 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    26761 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    26821 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    26881 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    26941 gtaaagcgca attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    27001 ttgaaacgtt ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag aaactggata
    27061 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    27121 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    27181 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    27241 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    27301 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    27361 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    27421 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    27481 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    27541 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    27601 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    27661 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    27721 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    27781 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    27841 ccctgctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    27901 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    27961 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    28021 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    28081 atatcttttc cttccgcgaa tgcggtcagc aagcctttgt cacgcgagag atgcgccata
    28141 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    28201 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    28261 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    28321 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aggtcgattt cagcttcgcc
    28381 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    28441 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    28501 ttttcaaaga gaatggtttg taactgcttg gtggaagaaa ggttaaattc ctcacctgca
    28561 atttcatgcg ctttcttttc cagctcagcc agacgaaggg tgagctcttc agaatgattg
    28621 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag cactggcacc
    28681 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    28741 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggct
    28801 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttaccagc aatctcttca
    28861 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    28921 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    28981 atgccgtagt tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    29041 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga gatttgatcg
    29101 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    29161 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    29221 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    29281 atggtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    29341 acactggttt cctgtggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    29401 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    29461 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacgtcggtt
    29521 ttaatcgtcg ccagctggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    29581 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggcatacagc
    29641 gtatccagtc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    29701 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaaatcgat gatcagttct
    29761 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    29821 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctgatc
    29881 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    29941 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcaagggt
    30001 tcgatttgtg cacgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    30061 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    30121 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    30181 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    30241 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    30301 attatgggta aaggatgcca cagacaggat aaaaacatga gtttttcacc gcttttcgtg
    30361 aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    30421 aaattattta catcgctttt aatttattat catgcttttt acagtatgat gtttgtcttt
    30481 gtttttcatg cagttaaaaa taaagccatc cctaataatt gagataatgg gattttttct
    30541 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcgtttt
    30601 ctgctaaaat ctgcgccttg cgcttcttta gcgcactgtc tatggctaac cttttgaata
    30661 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    30721 ttttggtcac tattttttgc tccgtcccga tcatcattgc cgggattgta aaactattgc
    30781 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    30841 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    30901 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    30961 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    31021 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    31081 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtaaag
    31141 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    31201 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    31261 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    31321 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    31381 tcgatatgtt aagcggtaaa ctgacgcgaa ttgtcgttca tgtggattta cagcctattg
    31441 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    31501 ggctgaactc actgtggcag gaaaaagacc gacttctgac gtcgttgatg tcatcacagc
    31561 ggcaggacaa gtaaattttg ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    31621 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    31681 aactttaccc ggtgtataac gcagttgcag cgaggcggta ttgttgtcaa tcgtgattaa
    31741 ctgaaacatc tgacctaagg tcatcatccc gctgatttgt ttatcagccc gcttttgcgc
    31801 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttctg tggcgacatt
    31861 aagcggtaac ttcacatcaa aattgagcga tttaatatct ttgtttgtgg atgatgaaga
    31921 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    31981 ccattttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    32041 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    32101 agcaagctgc ttctgcattg caatgttata ctggatgata aattggcgca ccgctgaggg
    32161 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    32221 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    32281 cagcatggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    32341 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    32401 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    32461 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    32521 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    32581 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    32641 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actaagttca cttttgccgc
    32701 tgccagtgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    32761 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    32821 atcgaatgtc atttgcattt gaaaacggga attaaaaaag cctttgtcaa agtttttata
    32881 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    32941 aaacttttcg acaccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    33001 tacggctaac gcaacaataa cacctgtagc tgacttatgt atcatataaa tattccctga
    33061 taaaacagca aaactttaaa tatccgctta tgctgaaata atgtccagcg gcaggacgca
    33121 ttatcagaat gtatttatat ttattaaata ataaaaaaaa cccgtgaata ttcacgggcc
    33181 ttatgtaatt tacattgaat tattttttct cggacagata tttcactgta tcagcatact
    33241 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    33301 cgttaacaaa catcgccgga acgccacgca attgcacgtc agctgcagct ttttcctgct
    33361 gagcgaccag agatttgacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    33421 tacctgcgtt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    33481 gtacgccttc aaacagcgga acagtgactt tgtcttccac gcccagcgcc atcgccacag
    33541 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    33601 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    33661 caaactgata gcagtgcgga cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    33721 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    33781 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    33841 gattaataca taggtgttaa ttgtaaaggg ggttcttgta gaacttttgc ctgttctata
    33901 aaagtcgccg tttgtcgcag ccagtaatct tccccggtta accacgggaa atttttcggg
    33961 aacgcgggat cagcccaacg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    34021 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    34081 tcaataatag tttccagttg catccgctgc tcggctttat cgccattgag caacatccac
    34141 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    34201 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaagtcttcc
    34261 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcaggaa tgccgctttc
    34321 aacccggaag gcatcagtgt agcgtcctca aacagcttgc gtggctcaat gagatattca
    34381 ttcagaccga tggtcgggcg atggataaaa agctgtttgc gccccgtctg gtgcatacgc
    34441 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    34501 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    34561 aaggccacag gtgctgcgac cggaacttca tcatttacca actgcaacgc aaattgatgt
    34621 tcttcgagga tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    34681 cgacgatctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    34741 ccggaatcca cccggatccc ttgctcaaat agagcatcca tgatggtatc cgggtgtagt
    34801 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    34861 aggatatcat ttcacgatga ttttggtggc accgcttaca agcttttact ctttaattac
    34921 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    34981 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    35041 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    35101 caggttaagg tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    35161 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    35221 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    35281 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgcttga attaaacggc
    35341 aaaccattat ggcaacatgt cgctgacgcg cttatgacgc agctctctca cgtcgtggtt
    35401 aatgccaatc gccatcagga aatctatcag gcaagcggtc tgaaagtgat tgaagattca
    35461 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagcaggc
    35521 gagtggtttt tgttttgtcc gtgcgatact ccttatattc ccgaggattt agcagcccgg
    35581 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc acgacggtga acgcgatcac
    35641 ccgactattg ctctggtaaa ccgcgcaatt gagcctttat tactggaata tctgcaagca
    35701 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    35761 gataataaag atgcatttat taacgtgaat acgccagagg agcttgcccg atggcaggaa
    35821 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    35881 tgaaacaact gatcccggca ttatgcgcca gagggatccg tccagggctg attaagcata
    35941 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    36001 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    36061 acgaagaaga gctggatcta cagtttctcg caagtcgaat ggatacctca aagctggatt
    36121 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    36181 gagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    36241 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    36301 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    36361 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggccca gtcttccgac
    36421 tgggccttct gcttaatttg atgcctggca gttccctact ctcgcatggg gagaccccac
    36481 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
    36541 cgcgctaagg ccgccaggca aattctgttt attaacccgc ttaccgccgg ttaattttat
    36601 ctgtatcagg ctgaaaatct ttctctctt
//
LOCUS       DAPPNJ010000021        36189 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000021 DAPPNJ010000000
VERSION     DAPPNJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.021"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            206..1579
                     /gene="aroP"
                     /locus_tag="RPM73_000806"
     CDS             206..1579
                     /gene="aroP"
                     /locus_tag="RPM73_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDX6519197.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1622..2476)
                     /gene="ampE"
                     /locus_tag="RPM73_000807"
     CDS             complement(1622..2476)
                     /gene="ampE"
                     /locus_tag="RPM73_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDX6519198.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2473..3024)
                     /gene="ampD"
                     /locus_tag="RPM73_000808"
     CDS             complement(2473..3024)
                     /gene="ampD"
                     /locus_tag="RPM73_000808"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDX6519199.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3112..4005
                     /gene="nadC"
                     /locus_tag="RPM73_000809"
     CDS             3112..4005
                     /gene="nadC"
                     /locus_tag="RPM73_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDX6519200.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4208..4648
                     /gene="ppdD"
                     /locus_tag="RPM73_000810"
     CDS             4208..4648
                     /gene="ppdD"
                     /locus_tag="RPM73_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDX6519201.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4658..6043
                     /gene="gspE"
                     /locus_tag="RPM73_000811"
     CDS             4658..6043
                     /gene="gspE"
                     /locus_tag="RPM73_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDX6519202.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6033..7235
                     /gene="hofC"
                     /locus_tag="RPM73_000812"
     CDS             6033..7235
                     /gene="hofC"
                     /locus_tag="RPM73_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDX6519203.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7270..8313)
                     /gene="guaC"
                     /locus_tag="RPM73_000813"
     CDS             complement(7270..8313)
                     /gene="guaC"
                     /locus_tag="RPM73_000813"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDX6519204.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8469..8513)
                     /locus_tag="RPM73_000814"
     CDS             complement(8469..8513)
                     /locus_tag="RPM73_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519205.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8538..9158
                     /gene="coaE"
                     /locus_tag="RPM73_000815"
     CDS             8538..9158
                     /gene="coaE"
                     /locus_tag="RPM73_000815"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDX6519206.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFANLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9158..9901
                     /gene="zapD"
                     /locus_tag="RPM73_000816"
     CDS             9158..9901
                     /gene="zapD"
                     /locus_tag="RPM73_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDX6519207.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9911..10108
                     /gene="yacG"
                     /locus_tag="RPM73_000817"
     CDS             9911..10108
                     /gene="yacG"
                     /locus_tag="RPM73_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDX6519208.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10208..10597)
                     /gene="mutT"
                     /locus_tag="RPM73_000818"
     CDS             complement(10208..10597)
                     /gene="mutT"
                     /locus_tag="RPM73_000818"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDX6519209.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10657..13362)
                     /gene="secA"
                     /locus_tag="RPM73_000819"
     CDS             complement(10657..13362)
                     /gene="secA"
                     /locus_tag="RPM73_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDX6519210.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13424..13936)
                     /gene="secM"
                     /locus_tag="RPM73_000820"
     CDS             complement(13424..13936)
                     /gene="secM"
                     /locus_tag="RPM73_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDX6519211.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14167..15084)
                     /gene="lpxC"
                     /locus_tag="RPM73_000821"
     CDS             complement(14167..15084)
                     /gene="lpxC"
                     /locus_tag="RPM73_000821"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDX6519212.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15185..16336)
                     /gene="ftsZ"
                     /locus_tag="RPM73_000822"
     CDS             complement(15185..16336)
                     /gene="ftsZ"
                     /locus_tag="RPM73_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDX6519213.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16397..17659)
                     /gene="ftsA"
                     /locus_tag="RPM73_000823"
     CDS             complement(16397..17659)
                     /gene="ftsA"
                     /locus_tag="RPM73_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDX6519214.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17656..18486)
                     /gene="ftsQ"
                     /locus_tag="RPM73_000824"
     CDS             complement(17656..18486)
                     /gene="ftsQ"
                     /locus_tag="RPM73_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDX6519215.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18488..19408)
                     /gene="ddlB"
                     /locus_tag="RPM73_000825"
     CDS             complement(18488..19408)
                     /gene="ddlB"
                     /locus_tag="RPM73_000825"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDX6519216.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQELVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19401..20876)
                     /gene="murC"
                     /locus_tag="RPM73_000826"
     CDS             complement(19401..20876)
                     /gene="murC"
                     /locus_tag="RPM73_000826"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDX6519217.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20930..21997)
                     /gene="murG"
                     /locus_tag="RPM73_000827"
     CDS             complement(20930..21997)
                     /gene="murG"
                     /locus_tag="RPM73_000827"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDX6519218.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21994..23238)
                     /gene="ftsW"
                     /locus_tag="RPM73_000828"
     CDS             complement(21994..23238)
                     /gene="ftsW"
                     /locus_tag="RPM73_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDX6519219.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23238..24554)
                     /gene="murD"
                     /locus_tag="RPM73_000829"
     CDS             complement(23238..24554)
                     /gene="murD"
                     /locus_tag="RPM73_000829"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDX6519220.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGLTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24557..25639)
                     /gene="mraY"
                     /locus_tag="RPM73_000830"
     CDS             complement(24557..25639)
                     /gene="mraY"
                     /locus_tag="RPM73_000830"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDX6519221.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25633..26991)
                     /gene="murF"
                     /locus_tag="RPM73_000831"
     CDS             complement(25633..26991)
                     /gene="murF"
                     /locus_tag="RPM73_000831"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDX6519222.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26988..28475)
                     /gene="murE"
                     /locus_tag="RPM73_000832"
     CDS             complement(26988..28475)
                     /gene="murE"
                     /locus_tag="RPM73_000832"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDX6519223.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28462..30228)
                     /gene="ftsI"
                     /locus_tag="RPM73_000833"
     CDS             complement(28462..30228)
                     /gene="ftsI"
                     /locus_tag="RPM73_000833"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDX6519224.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30244..30609)
                     /gene="ftsL"
                     /locus_tag="RPM73_000834"
     CDS             complement(30244..30609)
                     /gene="ftsL"
                     /locus_tag="RPM73_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDX6519225.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30606..31547)
                     /gene="rsmH"
                     /locus_tag="RPM73_000835"
     CDS             complement(30606..31547)
                     /gene="rsmH"
                     /locus_tag="RPM73_000835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDX6519226.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31549..32007)
                     /gene="mraZ"
                     /locus_tag="RPM73_000836"
     CDS             complement(31549..32007)
                     /gene="mraZ"
                     /locus_tag="RPM73_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDX6519227.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32609..33613)
                     /gene="cra"
                     /locus_tag="RPM73_000837"
     CDS             complement(32609..33613)
                     /gene="cra"
                     /locus_tag="RPM73_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDX6519228.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33695..33781)
                     /locus_tag="RPM73_000838"
     CDS             complement(33695..33781)
                     /locus_tag="RPM73_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDX6519229.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33793..34284)
                     /gene="ilvN"
                     /locus_tag="RPM73_000839"
     CDS             complement(33793..34284)
                     /gene="ilvN"
                     /locus_tag="RPM73_000839"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDX6519230.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34287..36011)
                     /gene="ilvI"
                     /locus_tag="RPM73_000840"
     CDS             complement(34287..36011)
                     /gene="ilvI"
                     /locus_tag="RPM73_000840"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDX6519231.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36130..36174
                     /locus_tag="RPM73_000841"
     CDS             36130..36174
                     /locus_tag="RPM73_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519232.1"
                     /translation="MKRNDKILHYEGLN"
BASE COUNT         8638 a        10158 c         8906 g         8487 t
ORIGIN      
        1 acggtttctt tttaagcaac tcatcttcaa ccatgcataa agcgggtgca ttcgctgccg
       61 cataccatta ttcttgatct gacggaagtc tttttgtaac aattcaaact tctttgatgt
      121 aaacaaatta atacaacaaa cggaattgca aacttacaca cgcatcactg cgtagatcaa
      181 aaaaacaacc accgcacgag gtttcatgat ggaaggtcaa cagcacggcg agcagctaaa
      241 gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg ggtggcgcga tagggaccgg
      301 gttattcctg ggtagcgcct ccgtaataca gtccgcaggg ccagggatta tcctgggtta
      361 cgccattgct ggttttatcg cctttctgat catgcgtcag ctgggtgaaa tggtggtcga
      421 agaacctgtc gcaggctcct ttagccactt tgcttataaa tactggggca gttttgccgg
      481 tttcgcctct ggctggaact actgggtact gtacgtttta gttgccatgg ctgagctgac
      541 tgccgtgggt aaatacattc agttctggta tccggaaatc cccacctggg tttctgccgc
      601 cgtattcttt gtggtgatta acgccatcaa cctgaccaac gttaaagtgt ttggcgagat
      661 ggagttctgg tttgccatta tcaaagttat cgcggtggta gcgatgatca tcttcggcgg
      721 ctggctgcta ttcagtggca acggcggccc gcaggcgacc gttagcaacc tgtgggatca
      781 gggtggtttc ctgccgcacg gcttcaccgg gctggtgatg atgatggcga ttatcatgtt
      841 ctcgttcggt ggtctggaac tggtggggat caccgcagca gaagctgata acccggagca
      901 aagtataccg aaagcaacta accaggttat ctaccgcatc ctgattttct atattggttc
      961 gttagccgtt ctgctctcac tgatgccgtg gacccgcgtt accgccgata ccagtccgtt
     1021 tgtgctgatc ttccacgagt taggcgatac ctttgtggcg aatgcgctga acatcgtggt
     1081 actgactgcg gcgctctccg tgtacaacag ctgcgtatat tgcaacagcc gtatgctgtt
     1141 tggtctggca caacagggta atgcgccaaa agcgctggcg tctgtcgata aacgtggtgt
     1201 accagtaaat accattctgg tgtctgcact ggtaacggcg ttgtgcgtac tgattaacta
     1261 ccttgcccca gagtccgctt tcggactgtt aatggcgctg gtggtatctg cactggtaat
     1321 caactgggcg atgattagcc tggcgcatat gaaattccgt cgcgccaagc aggaacaagg
     1381 cgtggtaact cgcttccctg ctctgcttta tccgctgggt aactggatct gcctgctgtt
     1441 tatggcggcg gtactggtga ttatgctgat gaccccagga atggcgattt cggtatacct
     1501 gatcccggta tggctgatcg tgttaggtat cggctatctg tttaaagaga aaaccgccaa
     1561 agccgtaaaa gcgcattaat ctctctacgc cctcacccgt acagggtgag ggcaataatc
     1621 tttacaccaa cgccccgtaa atcgtcagta gtgcaatcac caccacgacc acgaacgagg
     1681 ttttcttcgc cattgaaacc gctgccttcg gcgtctccac cttatcgaca tgcggttcac
     1741 gcgccagaga gaactgcgcc agacgcgtta acacctgata ctgcgaagta tggaaatcac
     1801 ccagcgaagc aaaccaggcc ggtaacgctt tctcaccatg accgatcaag gcatatacca
     1861 cacccgcaag acgaaccggc acccaatcca gtacatgaag cacggcatca atgccggact
     1921 gtaaacgatg atgcggcgtc tgatatcgcg ccagccagta ttgccatgca cgcaaaaacg
     1981 cataccccat cagcgtaacg ggtccccagg ttccccccac aatcagccag aacagcggtg
     2041 caagataaaa acgaaagtta atccacagca atgcattttg cagctcacgc aaatactcac
     2101 gttcgtcgca gcctgccggg acgccgtgaa tcatggtgag ttcgccagcc atcgtggcac
     2161 gggcatggct atcattacgt gaagcagctg tcagataagc atgataatga agacgaactt
     2221 tacctgcgcc aatacacagc aaaccaatca gcagccacac cagtagcgtg ggaacgttga
     2281 acaatactcc ctgcaatgcg cgtaacagta aaaaagtcac gcccatcgca ataatggtca
     2341 tgcctaacgt gcgcccgaga gaaaaatgtt tcacccgccg aaagaacgct tcaagacgat
     2401 gatcaagctg ccagtgctcg cccaacttaa acaggcgctc gaaaattaac accagtaagg
     2461 ttgtaaatag cgtcatgttg tctccttgct gaccagcaca cgaaaccgtg cccaatcaaa
     2521 tgcaggaccg ggatcggttt tccgatccgg cgcaatatca caatggcccg tcatgttttt
     2581 agcgatatcc ggatagcaat caatcagtgc ccgcgtaacc gccgcaagct gttgatactg
     2641 cgcatcggta tacgccagcg tatcggtgcc ttcaagctca atcccaatag aaaaatcatt
     2701 gcagcgttcg cgcccctgat actgagagac tcccgcatgc catgcacgtt tatcgaaagg
     2761 aacatactgg actatttcac catcacggcg aatcaaacag tgagcggaga cgcgcaaatg
     2821 ggcgatctca gcaaagaaag gatgtgcctg cggatcaata gttccagtga ataatgcgtc
     2881 gatccacgga ccgccaaact cgcctggcgg caggctaata ttgtgcacca ccagcagggt
     2941 gggtgtttcg tcatccgggc ggcaatcgta atgtggtgag ggaacgcggc gcgcgccaac
     3001 cagccacccc tgttctaaca acatgcagga tctccttata tgtggtgcta atacccggtt
     3061 cagagtagca tgtttctacc ttatgattcg ttagctatct ggagttttaa catgccgcct
     3121 cgccgctata accctgacac ccgacgtgac gagctgctgg aacgcattaa tctcgatatc
     3181 cccggcgcgg tggcccaggc gctgcgggaa gatttaggcg gaacagtcga tgccaacaat
     3241 gatattacgg caaaactttt accggaaaat tctcgctctc atgccacggt gatcacccgc
     3301 gagaatggcg tcttttgcgg caaacgctgg gttgaagagg tgtttattca actggcaggc
     3361 gacgatgtca ccataatctg gcatgtggat gacggcgatg tcatcaatgc caatcaatcc
     3421 ttgttcgaac ttgaaggccc atcccgcgtg ctgttaacgg gcgaacgcac tgcgcttaat
     3481 tttgtgcaaa ccctttcagg agttgccagt aaggtacgcc actatgtcga attgctggaa
     3541 ggcaccaaca cgcagttgtt ggatacgcgc aaaaccttac ccggcctgcg ttcagctctg
     3601 aaatacgcgg tactttgcgg cggcggagcg aatcaccgtc tggggctttc tgatgccttc
     3661 ctgatcaaag aaaaccatat tattgcctcc ggctcagtgc gccaggcggt cgaaaaagcg
     3721 tcctggctgc acccggatgc gccagtagaa gtcgaagtag agaatctgga agaacttgat
     3781 gaagccctga aagcaggagc cgatatcatc atgctggata acttcgaaac agaacagatg
     3841 cgcgaagccg tcaaacgcac caacggcaag gcgctactgg aagtgtctgg caacgtcact
     3901 gacaaaacac tgcgtgaatt tgccgaaacg ggcgtggact ttatctccgt cggtgcgcta
     3961 actaaacacg tacaagcact cgacctttca atgcgttttc gctaatagtt gacagattta
     4021 tccgctccat cgcgggcgga taatctcccc ttccccaact ttcttcgtaa cgcctcgcaa
     4081 atttcttgtc tttcgctgca acgaaataaa aaacctcgga atctgctttc ccgtttcgtt
     4141 ttttgcccct cgcctgtgct gatggcgttt gccaaagtag cgccaaccaa atcaaggagc
     4201 gaaacagatg gacaagcaac gcggttttac acttatcgaa ctgatggtgg ttattggcat
     4261 cattgccatt ttaagcgcca ttggtattcc cgcttatcaa aactacctgc gcaaagccgc
     4321 actcaccgac atgctacaaa cctttgtgcc ttaccgtacc gccgtagagt tgtgcgcgct
     4381 ggaacatggt ggattagata cctgcgacgg tggcagcaat ggcattccct cgcctaccac
     4441 cacccactat gtttcagcca tgagtgtggc aaaaggcgtg gtgtcgctga ccgggcaaga
     4501 aagtctcaat ggactaagcg tcgtcatgac accgggttgg gataacgcaa acggcgtcac
     4561 cggctggacg cgcaactgca atattcaaag tgacagcgca ttgcagcaag cctgcgaaga
     4621 tgtcttccgc tttgatgacg ccaactaagg agcggcaatg aatattccac agctcacggc
     4681 cctgtgtctg cgttatcagg gagtcttgct ggatgccagc gaagaagtgg ttcatgttgc
     4741 ggtggtcgat gccccctcac atgagttgct ggacgcattg catttcgcta ccaccaaacg
     4801 tattgagatc acctgctgga cgcgccaaca aatggaaggt cacgccagtc gcacacaaca
     4861 gacattgccc gtagctgttc aggagaagca tcagcccaaa gcagagttgc tagctcgaac
     4921 gttacaatct gcgctggaac aacgcgcgtc tgatattcat atcgaaccag cggacaatgc
     4981 ctaccgcatc cgcttgcgta tcgacggcgt attgcatcct ttaccggatg tttcaccgga
     5041 tgccggagtc gcattaaccg ccagattaaa agtgctggga aacctggata ttgcggaaca
     5101 tcgcctgccg caggacgggc aattcactgt cgaactggca ggaaacgccg tctcatttcg
     5161 tattgcgacc ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt tacagcaggt
     5221 gggtcaggca ctggatgtca acacgcttgg aatgcagccg ttacaactgg cggactttgc
     5281 tcatgccttg caacaaccac agggactggt gctggtaact ggccctacag gcagcggcaa
     5341 aacggtcacg ctttatagtg ccctgcaaac gctgaatacc gctgacatta atatttgtag
     5401 cgtcgaagat ccggttgaga tccccatagc cggactaaac cagacgcaaa tccatccgcg
     5461 tgccggactc acctttcagg gcgtgttgcg tgcgttattg cgccaggatc ctgacgtcat
     5521 catgatcgga gagatccgcg atggcgaaac agcagagatc gctattaaag cggcgcaaac
     5581 tggtcacctg gtgttgtcta ccctacacac taattccacc tgcgaaacgc tggtacgttt
     5641 acagcaaatg ggagtcgccc gctggatgct ctcatcagcg cttacgctgg taatagccca
     5701 gcgtctggta cgtaaacttt gcccacattg tcgccagcag caaggggagc ccatccatat
     5761 tccagtcaat gtatggccgt cgccgctgcc ccactggcag gcacccggtt gtgtacattg
     5821 ctaccacggt ttttatggtc gtacggcctt atttgaagtt ctgcccataa cgccggtcat
     5881 tcgtcagctt atttccgcta ataccgacgt tgaatcgctg gaaacgcacg cacgacaggc
     5941 gggtatgtgt acgctttttg aaaacggctg cctggccgtg gagcaaggct taaccacctt
     6001 tgaagagtta atccgcgtac tggggatgcc gcatggcgag taagcaactc tggcgctggc
     6061 atggcataac cggcgacggc aatgcgcaag atgggatgct atgggcagag agccgtgctt
     6121 tgctgctcat ggcactacag caacagatgg ttaccccact tagcctgaag cgaatcgcca
     6181 tcaattctgc gcagtggcga ggagataaaa gcgcggaagt cattcatcaa ctggcgacgc
     6241 tactcaaagc cgggttaacg ctttctgaag ggctggcact gctggcggaa cagcatccca
     6301 gtaagcaatg gcaagcgttg ctgcaatcgc tggcgcacga tctcgaacag ggcattgctt
     6361 tttccaatgc cttattaccc tggtcagagg tatttccgcc actctatcag gcgatgatcc
     6421 gcacgggtga actgaccggt aagctggatg aatgctgctt tgaactggcg cgtcagcaaa
     6481 aagcccagcg tcagttgacc gacaaagtga aatcagcgtt acgttatccc atcatcattt
     6541 tagcgatggc aatcatggtg gttgtggcaa tgctgcattt tgttctgccg gagtttgccg
     6601 ctatctataa gaccttcaac accccactac cggcactaac gcaggggatc atgacgctgg
     6661 cagactttag tggcgaatgg agctggctgc tggtgttgtt cggctttctg ctggcgatag
     6721 ccaataagtt gctgatgcgc cgaccgacct ggcttatagt gcggcagaaa ttgctgttac
     6781 gcatcccgat tatgggttca ctgatgcggg gacaaaaact cacgcagatt tttacgattc
     6841 tggcgctgac acaaagtgca ggcattactt ttttgcaggg cgtagagagc gtcagagaaa
     6901 caatgcgctg cccgtactgg gtgcaacttc tgacacaaat ccagcacgat atcagtaacg
     6961 gtcatcccat ctggctggcg ctaaaaaata ccggggagtt tagcccgctc tgtttgcaat
     7021 tagtgagaac aggagaggca tccggctcgc tggacctcat gttagacaac ctcgcccatc
     7081 atcatcggga taacacaatg gcgctggcgg ataacctcgc agccttactg gaaccggcgt
     7141 tgctgatcat aacgggagga attatcggta cgctggtggt ggcgatgtat ctgccaattt
     7201 tccatttagg cgatgcgatg agtgggatgg gataacgctg gcgtgttgct ccacaccagc
     7261 gttggaagat tacaggttgt tgaagatgcg gttttcttgt tcctgcacac ggataaatgt
     7321 ggtgcgcttg gtcagttctt tcaggcgtga agccccaacg tatgtacaag ctgaacgcag
     7381 gccgcccaaa atatcacgcg cggtattttc aaccgggcct cgcagcggca gcttaacggt
     7441 tttaccttct gctgcgcgat attccgcaac gccgccaacg tgacgtttca tcgcagactc
     7501 ggagctcatg ccgtagaaca gcataaattt ctcgccgttc tcctcaacga tgcgaccgcc
     7561 gctctcttcg tggcccgcca gcatgccgcc aagcatgacg aaatcggcac cgccgccaaa
     7621 ggcttttgcg acatcgcccg gcgtggtgca gccaccgtcg ctgacgatca ttccgcccag
     7681 accgtgcgcc gcatcggcac attcgattac cgcagaaagc tgcggataac cgacgcctgt
     7741 tttgacgcga gttgtacaaa cagaacctgg gccaatgcca actttaacga tatcagcacc
     7801 ggagaggata agctcctcac acatttcacc agtcactacg ttgccagcac aaatggtttt
     7861 ggtcggccac gcttcacgcg ctttcgcaac gaactgcacg aagtgttcgg aataaccatt
     7921 cgccacatca atacaaacga agtttaatgc cgggttcagg tcgagaatct gtttagtttt
     7981 ttcgaaatcc gcatcagacg taccggtaga aaccatcaca tgtttcagca catcagcgga
     8041 agaattgttg ataaacgctt gccactcttc gacagaatag tgtttatgca cagcagtcaa
     8101 aatatcaaaa gaagccagcg cagaggccat agaaaatgta cctacggtgt ccatatttgc
     8161 ggcgataatt ggcacgccgg accagctctg acctgaatgt ttgaaggtga attgacgttc
     8221 cagttcaaca tcggaacggc ttttaagagt ggagcgttta gggcggatga gaacgtcttt
     8281 aaaacctaac ttcagatctt cttcaatacg catgtgcgga ttcctggggt taatggctat
     8341 aagggtaaga gcgcaactcc agtgacgcta tcatacgcac taatcaatgc ggcgcaagac
     8401 tgcgaaattg tcttttttta cgctacaatc ccataaattt acctggtaaa tagtgagcga
     8461 acgtttcatc aatttcgctt tcgtgcgctc atttttaacg ttttgataat catgatgaat
     8521 tcccgggaat ataaattatg aggtatatag ttgccttaac gggaggcatt ggcagtggca
     8581 agagtaccgt tgccaatgcg tttgctaatc tcggaattaa cgtcattgat gccgatatta
     8641 ttgcgcgtca ggtggttgaa ccaggtgcac ctgcgctaca tgccattgct gatcactttg
     8701 gcgctaacat gattgctgct gatggaacat tgcagcgccg ggccttgcgc gagcggatct
     8761 tcgccaaccc ggaagagaaa aactggctta acgacctgct gcatccgctg attcagcaag
     8821 agacgcaaca ccagatccag caagcaactt ccccctatgt actgtgggtt gtgccattgc
     8881 tggtagaaaa ctcactgtat aaaaaagcga atcgagtgct ggtggtggat gtcagcccag
     8941 aaacgcaact taagcgcacc atgcagcgcg atgatgtaac tcgcgaacat gtcgaacaaa
     9001 tccttgctgc tcaggcaacg cgcgaagccc gccttgccgt ggcagatgac gtcattgata
     9061 ataacggcgc accggatgct atcgcatcgg atgttgcccg cctgcacgca cactatttgc
     9121 agcttgcgtc gcagtttgtc tcacaggaaa aaccgtaatg cagacccagg tcctttttga
     9181 acatccacta aatgaaaaaa tgcgtacatg gctgcgcatt gagtttttga ttcagcaact
     9241 caccgttaat ttacccatcg ttgaccacgc tggcgcgctg catttcttcc gtaatgtcag
     9301 tgaattactg gatgttttcg agcgcggcga agtccgcact gagctgttga aagaacttga
     9361 ccggcagcaa cgtaaactcc agacctggat tggcgtgcct ggcgtggacc agagccgtat
     9421 tgaagcatta attcagcagt taaaagcggc ggggagcgta ttaatttccg cgccgcgtat
     9481 cgggcaattt ctgcgtgaag atcgtttgat tgctctggtg cgtcagcgac tgagcatccc
     9541 aggcggctgt tgcagctttg atttacctac attgcacatt tggctgcatc taccccaggc
     9601 gcagcgcgac agccaggtag aaacctggat tgccagcctg aacccgctca cccaggcact
     9661 taccatggtg ctggatttaa ttcgccagtc ggcccccttc cgtaaacaaa ccagcctgaa
     9721 tggtttttat caggataacg gtggcgatgc cgacttgctg cgcctgaatc tgtcgctcga
     9781 ttcacagctt tatccgcaaa tttccggaca taagagccgt tttgccattc gttttatgcc
     9841 gctggacact gaaaacggac aggtaccgga acgtctggat ttcgaactgg cctgttgcta
     9901 aggagtgaaa atgtcagaaa ctattacggt gaattgccca acctgcggga aaacggtggt
     9961 gtggggtgaa atcagcccgt ttcggccatt ttgctccaaa cgttgtcagc tgatcgacct
    10021 cggagaatgg gctgctgaag aaaaacgaat ccccagcagt ggcgatcttt ccgaaagcga
    10081 tgactggagc gaagagccaa agcagtgaca tttgtctgat gccgcacgta ggcctgataa
    10141 gacgcggaca gcgtcgcatc aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt
    10201 atccgaccta cagacgctta agcttcgcaa ttaccggttc attggctggc ggaaaatcat
    10261 cggcattaag accgactagc gacatccact caccgggttg cccttcttta ccccacggct
    10321 ccccttccca gctttcgacc agccagaacc acagagttat atgcctgtcc gggaattcat
    10381 attccagttt ttcaaatagc gaaaaatgtt gtggggtaat cccgacttct tcctgaagtt
    10441 cacgcaccac cgcctgttcc ggcgtttcgc ccatttcaat tttaccgccg ggaaactcca
    10501 gtttattcgc catgtgcgca tctgctgcgc gacgcgttat aaagatttca ttgttctcgt
    10561 tgcgaataat acctaccgca atttgcagct ttttcattgt cttaaaccta taaaaaaggc
    10621 gcagaatcct gcgcctttta cttcaactgt tagcttttat tgcaggcgac catggcactg
    10681 cttgtatttt ttaccagaac cgcacgggca aggatcgtta cgtcctactt tgcgctcacc
    10741 ggtttgcgcc gccagtgcag ctgcggctgc agagtcgtca tcctgatggc taagctgctg
    10801 catttgcgct aaacgctcgg cttccatacg acgctgttgt tccagctcct caacctcttc
    10861 aggcatacgt acctggactt tgctcagcgt gctgataact tcatatttta acgactccag
    10921 catcgctgca aacatggaga acgattcacg tttgtattcc tgcttcggat ctttctgtgc
    10981 atagccacgc aggtggatac cctgacgcag atagtccatc gccgccaggt gctctttcca
    11041 cagagagtcg agagtttgca gcatgacgcc tttttcgaag tgacgcatca tctcagcacc
    11101 aaccacttct tctttacgct gatacacttc gatggactgc gccagaattc gctcacgcag
    11161 cgtctcttca tgcagttctg gttctttatc cagccactcg gcaattggca aatcgaggtc
    11221 gaaatcgttc ttcagacgtt cctgcagccc cggaatatcc cacatttctt ccagtgactg
    11281 tggcggaatg taggcatcaa tggtcgcttt gaacacatct tcacgaatgc tgttgatggt
    11341 ttcgctcaca tcgctgacat ccagcagttc gttacgctgg gagtaaatgg cgcgacgctg
    11401 atcgttagcc acgtcatcat attccagcag ttgcttacga atgtcgaagt tacgactttc
    11461 aactttacgc tgggcgttgg caatcgcttt agtcacccac gggtgttcaa tggcttcgcc
    11521 tggcttcata cccagtttac gcatcatgcc ggatactcgg tcggaagcaa aaatacgcat
    11581 cagcgcatct tccatcgaca ggtagaaacg ggaagaacca gcatccccct gacgaccaga
    11641 acgaccgcgc aactggttat cgatacgacg ggattcgtga cgctcggtac cgatgatatg
    11701 caggccacct gcttccagta ccgcatcgtg acgtacctgc cagtcggctt taattttttc
    11761 aatttgctct gcggtcggat tttccagcgc ggcaacttct gcctgccagc taccaccgag
    11821 cacaatatct gtaccacgac ccgccatatt ggtcgcgata gtcaccgcag ccggataacc
    11881 tgcctgagca acaatcgccg cttcgttggc gtggaatttg gcgttcagga cgttgtgctt
    11941 aataccggct ttggtcagtt cgtttgacac cagctccgat ttttcgatgg agatagtacc
    12001 caccagcacc ggctggcctt tcgcagtacg ttctttgata tcttcaatga tcgcctgaat
    12061 tttttccgct tcagtcatgt agaccaggtc cggcagatct ttacgaatca ttggacggtt
    12121 ggtcggaaca acgacggtat ccagcttgta gatggagcta aattcgaaag cttcggtatc
    12181 agcagtaccg gtcatccccg ccagtttttc atacagacgg aagtagttct ggaaggtgat
    12241 cgaagccagc gtttggtttt cgttctggat ctgcacacct tctttcgctt ccacagcctg
    12301 gtgcagacca tcggaccagc gacggccctg catggtacga ccggtgtgtt cgtcaacgat
    12361 gataacttca ccatctttaa cgatgtagtc gacgtcgcgg gtaaacagcg catgagcgcg
    12421 cagcgccgcc gttacgtggt gcatcagcat gatgttggcc ggagagtaca gagactcccc
    12481 ttcatccatg atgccctctt tcaccagcag ttcttcaatc agcaccagac cacgttcggt
    12541 caggttcacc tggcgagatt tttcgtccac cgagaagtgg ccttcgccct ggaaggtttc
    12601 ggagtcttct ttttcctgac ggatcaggtg cggaataatt ttattcacgc gtttatacat
    12661 ttccgagctg tcttctgccg ggccggaaat gatcagcggt gtacgcgctt catcgatcag
    12721 gatggagtcc acttcgtcca ccagcgcata gtgcagttta cgctgtacac gttcttcagg
    12781 gctgaacgcc atattgtcgc gcaggtagtc aaagccgtat tcgttgttcg taccgtaagt
    12841 gatgtcagct gcgtaagctt cgcgctttgc cggtgctggc atgcccggca ggttgatacc
    12901 gacagtcagg ccaaggaatt caaacagcgg acggttgttt tcggcgtcac gttgcgccag
    12961 gtagtcgttg acggtaacta cgtgcacgcc tttaccggtt agtgcgttca ggtaagcagg
    13021 cagcgttgcg gtcagggttt taccttcacc ggtacgcatt tcggcgatgc agcgttcgtt
    13081 aagaaccata ccgccgagta actgaacgtc gaagtgacgc ataccaaaga cgcgcttact
    13141 tgcctcacgt accacggcga aagcttccgg gatcagattt tccagcactt cgcctttttc
    13201 cagacgtgca cgaaactctg cggttttccc tttcagttct tcgtcggaga gtttttccat
    13261 ctccggttcc atggcattga tgatgttgac cactttgcgc atccggcgca gggtgcgatc
    13321 gttacgacta ccgaaaactt tagttaacaa tttgattagc ataataaaat ctcaaacgcc
    13381 ccgcgttgcg gagttaataa aatgaagtaa aggtttattg ttgttaggtg aggcgttgag
    13441 ggccagcacg gatgccttgc gcctggctta tccagacggg cgtgctgaat tttgcttgtg
    13501 gggtaaaatg cgcataatca atgcgataac ccttttcaga cggcgtgcct tcctgggtca
    13561 gcagcgcgct gagcgtatcc agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt
    13621 cagcaacggg cagtgtttgc ggtgccattg cgaaagaaag atgacggatt accgtgcgaa
    13681 tggcatgttg atgccagtaa tcaacggaat agttcgaatt cgggcggcgc gtgttcgctt
    13741 ccagcaaggc caattgacca aagttaactt tggctgaagg ctcgtggttg cgggttgtcg
    13801 cttttgcggg cgcgtttggt tcggcggcgt tgctgagcgc aggcaaacct aaactcgccg
    13861 caaccatccc taataagaga tgcggccaga agtagcgttt accaaactgt cgccagcgcg
    13921 tcagtattcc actcacgtta ttgccatccc gatcatattc aaacgtattt agcgcacaaa
    13981 tcttatcatt aaagcctgca gtccacagca cgaatgaaga taagttgtgc ggaaaagtgc
    14041 ttaagaaaac agcgttcgca ccgtttttta tagctcttat aaaattcaaa aaaaaacgac
    14101 tggttcacct ggccggagag agtgccagat ttgccagtcg aattttatac gacagtataa
    14161 atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc
    14221 ctggaaggtc acatattccc aggcttcttg tttcgccagg acagcctgca gcagtttgtt
    14281 attcagtgca tgaccggatt tataagcggt aaatgcacca ataatattgt gaccacacat
    14341 gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca aattcgtctt caaaacgcag
    14401 gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg gcacaatcga agctgccgcc
    14461 caggcacaaa ccacgggact gcagatattc gatatcacgc atgaaaccga acgtacgcgc
    14521 acggctgatc tggcgcataa acgcatcagc ggagaagttc atcgcatagc gctggttgct
    14581 ggaatcaata gccggatggt taaaatcgat ggtgaaatcc agcgaaaaac cattgtacgg
    14641 cttaaattca gcccacttat cgccatcttc gacacgaaca gtctctttga tgcgaacaaa
    14701 ttttttggcg cagttcaact cgtcgatacc ggcgtcaagc agcaggtata caaacggagc
    14761 ggcgctgccg tccatgatcg ggatttccgg cgcgttaact tcgataacaa tgttatcgat
    14821 gcccaggccc gcgagagcag cattgaggtg ctctacggtt gaaatccgta catcatgctc
    14881 gttgaccaga cacgtacaga gcatggtatc acgcacagat ttggcatcgg ccgggaaatc
    14941 taccggtgga ttcaagtcgg tgcgacgata gatgaccccg gtgttggccg gcgcagggcg
    15001 taacgtcagg gtgactttct tgccggtatg taaaccgaca cccgtcgcct gaacgatacg
    15061 tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa attacctatc caaccgaagt
    15121 gtactataca ttcggcgggc cagtttagca caaagagcct cgaaacccaa attccagtca
    15181 attcttaatc agcttgctta cgcaggaatg ctgggatatc cagataatcc ggctctttcg
    15241 cagtttgcgg cgcattgtca ttcacgactt tagcaaccgg cttctgctcc tgggtcagcg
    15301 gagccatccc atgctgctgg tagcgatcca tcactggctg ctgaacctgc ttattggtca
    15361 ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt cgcaacaacg gttacgcgca
    15421 gctcgtcatt catatccggg tcaagagaag taccgataac cacagtcgcg ttgtcggaag
    15481 caaatgcacg gatggtgtta cctaccgttt cgaactcatc cagacgcagg tcgaagcccg
    15541 ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc gatatcttcc agcagcggag
    15601 aagagatagc catttcagca gcttcttccg cacggtcttc accgctcgcc acgccagaac
    15661 ccatcattgc gtagcccatc tcagacatta cggtgcgtac gtctgcaaag tccacgttca
    15721 tcaaacccgg acgagtaatc agttcagcga taccttgcac agcgcctttc agtacatcgt
    15781 tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc cagaactttc agcagtttgt
    15841 cgttcgggat agtgatcaga gagtccacat gcttggacag ttcagtgatc ccctgctccg
    15901 cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt agtgacgaca gcaacggtca
    15961 ggatacccaa atcttttgcc acttcagcga cgactggtgc tgcacctgta ccggtaccac
    16021 cacccatacc cgcagcaata aagaccatgt ctgcaccttc cagcgccgca cgcaatgcat
    16081 cgcgatcctc atcagccgca ttgcggccaa cttctggatt agcgccagcg cccagtcctt
    16141 tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg
    16201 catcggtatt taccgcgaag aattcaacac cttcaatgcg ctcgcgcacc atgtgttcaa
    16261 cagcattacc gccgccgccg ccgacgccga tgactttaat caccgcgtca ttggtaagtt
    16321 ccattggttc aaacatagtt tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc
    16381 gtcggcctca taaaaattaa aactcttttc gcagccaact attgagtcgc ttgatccacg
    16441 agccaactga tgctgtaaca cgtttttcta cttcagcttc accgttaaga tgtgactctt
    16501 tcccatagtg aagcaatccc accgccgtcg aataatacgg ctcctgagca taatccgtta
    16561 aaccggtaat gttcagcggc gcgccgatac gcacttgcgt atgaaacacg cgctgagcac
    16621 aggctgcaag accttcgatc tgcgctgcgc caccggttaa tacaatgcct gccgccaggt
    16681 gatgtttaac cccttgttgg cgaagctttt cctgcaactg caatatctct tcgttgacca
    16741 ggttgagcag ctcggtatag cgcggctcga tcacctctgc cagggtctga cgttgcagac
    16801 tccgcggcgg acgaccacct acgctcggca cttccacgct ctcatctttt ccaacgatgg
    16861 aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc gtcgcttggc ggcgtgccaa
    16921 aggcgtaagc gatatcactg gtcacgacat tgccagcata aggaattacc ttagtgtggc
    16981 gcaatgcccc accggtataa acggcgatat ccattgtacc accaccgata tcgacgacgc
    17041 agacacccag ttcacgttca tcttccgtca ataccgaata acttgatgcc agtccggcaa
    17101 atatcagttg gtcaactttc agcccacaac gttcaaccgc tttgacgatg tttttcgcca
    17161 tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat ccgcacgccc gaaagtccta
    17221 ccggattctt gatcccttcc tgatagtcaa tcgcatactc ttgcgggatc acatgcagca
    17281 cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg gacgacgttt tccacatctt
    17341 cttgcgtcac ttcttcttca gaaataggca ccataccaat ttcattctgg cagctgatgt
    17401 gcttaccaga aagcgccaga tataccgaag agatctgaca atctgccatc aattctgcct
    17461 ggtcaatggc gcgttgtacg cacttgacca cggattcgag gtcgttcacc ccgcctttat
    17521 ccataccacg tgacgggcag ctgcccacgc caatgatatt gaccataccg tcgggcagaa
    17581 cttcccctac taaagcggca accttcgcgg taccaatctc cagtcctact accagttttc
    17641 tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga ttttgttgct gagtagattc
    17701 ctctggcggc aagggcgccc agcctactgc cgctccagag tcataacgca aatcaacgta
    17761 gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa accggataaa gttctacaaa
    17821 gcgagccaaa cgtttcatcg tatcgccccg gccaagattg agcttaatat cgttattcag
    17881 cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt
    17941 tgccagcatc tgccccattt cgcgatagcc ctgcaacact tcattggcgc tgccttccgg
    18001 gccatacagc attggaagca cctgcttgct ggtgcgatct ggcggcacgc tgaaggtgtt
    18061 tccttccgcg tctaccatgt gttgatcatt ccaccgcgca atcggcacat attcaaccag
    18121 atgaatcttc aattcatcag gccactgctt tctgacgctc acctgcttaa tccacggcag
    18181 gcgttgttct atttgcgtct ggatgatgtt gacatcctgg gtcataaagg tacccggctc
    18241 acccaatgcc aggatcgact gccggatatc gtcattacgc gtgtaatggc gttcaccggt
    18301 caacaccagc tttgagagcg gcaggcgttg cgcatcttcc atccagccca acacgaccca
    18361 gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt
    18421 tccattattg cggcgagaag aaacctcttc ttcgctgttt cgcgtgttca gagcagcctg
    18481 cgacatatta gtccgccagt tccagaattc gtactaccaa ctgcgagaag ctcatacctg
    18541 cctgacgtgc cgccatcggc accaggctgt ggctggtcat acccggtgag gtattggctt
    18601 ccagcagata aaactgtcca tcgctgtcca gcataacgtc aatacgtccc catcctttgc
    18661 aacctaacgt cgtccatgct ttcagcacta attcctgcaa attggcctct tgtgacgctt
    18721 ccagacctgc ggggcagaaa tactgtgtct catcagagag atacttcgcc tcataatcat
    18781 agaaggttcc ggacggttga atacgtattg acggtaaaat ttcttcaccg agtatcgcaa
    18841 ccgtgaactc cggcccactt agccattttt caatcaatac ttcttcatcg tgctgaaatg
    18901 ccaatcttaa tgcatcttgt agagcatttt ctgctactac ttttgacatt cccacactgg
    18961 aaccttcgcg gctcggctta acgataaccg gcaaacccag agcagaaatt tctgctaact
    19021 gcttatcact caggcctttt tcaaactctg cgcgggttaa cgcaacccac ggagcgaccg
    19081 gtaaaccggc accttgccac agcagtttgc tgcgtagttt atccattgaa agcgcagatg
    19141 ccatcactcc gctgccggta taaggcaagc ccatcagctc gagcatgccc tgcagcgtac
    19201 catcttcacc gccgcgaccg tgtagcgcga taaacacttt ctgaaaaccc atcgacttca
    19261 gttgcgtcac gtcgacttct ttcgggtcga caggatacgc atcaataccg ccttcacgca
    19321 gcccggctaa caccgctgcg ccagaattca gagaaacttc ccgctcagcg gaagtcccgc
    19381 ccaacaggac cgcgatttta tcagtcatgt tgttcttcct ccggagtttg cggcttcagt
    19441 ttgatttcag ctaaagaacg ggcaattttt ccgatattac cagccccctg aacgagaatc
    19501 aggtcgttac cggttaatac cggtgccagc atctcggcta cctgcgcagg gtccggcacc
    19561 agaatgggat caattttccc acgtccacga attgtgcgac acagcgaacg gctgtccgct
    19621 cccggaattg gcgcttcgcc agccggatac acttccagca tcaacagggt atcaacctgc
    19681 gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac gggtaaaacg gtgcggctga
    19741 aacagcatta ccaggttttt atccggccag cctgcgcgcg ccgctttaat ggtggcgtca
    19801 acttccgtcg ggtggtggcc gtagtcatcg accagcattg ccgtaccgct tttaccattc
    19861 actggctcca gcgggaattc accgaggaaa tcaaaacggc gcccagtccc ctggaagctc
    19921 tccagcgcac gtaaaatagc ctcgtcatca attccctctt ccgtagcaac cgcaaccgca
    19981 gctgcggcgt tcagcgcgtt atgacgacct ggcgcattca gggtgacgcg catcggctct
    20041 ttgtcctggc gcagcagcgt aaagtgcccc tgcgggccaa tctgctgata atcttctaca
    20101 cgcacgtcgg catcttcgct gaagccgtaa gtcgtggtct gacgtcccac tcgcggtaac
    20161 aattcgcgga tcaccggatc atcaacacac atcaccgcac gaccgtaaaa cggcaggttg
    20221 tgcagaaaat taataaaagt ctgttttaaa ttctcaaagt cgccctggta ggtatccatg
    20281 tggtcggctt cgatattggt gacaatcgcc accatcggtt gcagatgcag gaacgatgca
    20341 tcactctcat ctgcttcggc aatcaggtac cgaccatgcc ccaaacgcgc atgaaccccc
    20401 gccgctttta ccagcccgcc gttaacgaag gttgggtcga gccccgcttc tgcgtagatg
    20461 ctggaaacca tcgcggtggt tgtcgttttg ccgtgcgttc ctgcaatggc gatgccatga
    20521 cgaaaacgca ttaactcagc cagcatttcg gcacgacgga tcaccggaat acgcgcttca
    20581 tgagctgcga caatttccgg gttatcggca gaaatggcgc tggaaacaac gaccacgctg
    20641 gcatcacgta cgttttccgg gcgatggttg aaataaatcg tcgctcccag attcattaac
    20701 tgctgcgtga ccggatttgg cgctaaatcg gaaccactga tctgataacc ttcattggcc
    20761 agaacttcgg caataccgcc cataccggca ccaccgatgc cgacaaaatg tatgtgccga
    20821 acgcgacgca tttcgggcac gatggaacgc agttttgcca attgttgtgt attcattctt
    20881 tacgccatta acttcttaaa ttcatacgat gcaaaaggca tcgctacaat tacgcccggg
    20941 cagcccggct cacttcattt gccactcgct cggtggcatc cggaatggat gcagcgcggg
    21001 cgcgttctgc catggttaat aaggtttctc gcgaccaccc ggccagggtg ttggcgacag
    21061 catccacgct aagctgtggc tgctcgataa ttttggctgc gcccgctttt tccagcggta
    21121 gcgcattcca gtattgctgg cggtctttat gttgaaacgg cacaaacaac gccggtagtc
    21181 ctgccgcggc gatttcactc accgttaaag caccggagcg gcaaacgacg acatccgccc
    21241 acgcatacgc cgccgccata tcatcaataa attccgtcac tttatgctgc ggttgccccg
    21301 cttcggcata cgcctgttca acggattgtt gcgaaccttt accgctctga tgccagatag
    21361 tgactgaatc acccagtttc gcagcaacct gcggcattgt ctggttaaga atgcgtgcgc
    21421 cctgggaacc acccactacc agcacacgaa ccggaccttc acgtccagcc aaacgttgct
    21481 gcggcaacgg cagcgccagc acatcggtac gcaccgggtt acccaccact tccgcattag
    21541 ggaaagcgcc tggaaacgcc tgcatcactt tggtggcaat cttcgccagc catttattgg
    21601 ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg aatgcctaac gaccacgcgg
    21661 ccagaccacc tggacctgat acatagccgc ccataccgag taccacgtca ggtttgtacg
    21721 ctttcataat cgcccgcgcc tgacgccagg cgttgaagat acgcagcggc gcagctatca
    21781 gtgcttttat accttttcca cgcagaccag agatacgaat gaaatcaatt tcgatgccat
    21841 gttttggcac taagtccgct tccatacggt cggcagtccc cagccagcga acttgccaac
    21901 cctgagccat tagatggtgc gcaaccgcca gtcccgggaa tacatgtcca ccggttccgc
    21961 ctgccatcac cattaatcgc tttccttgac cactcatcgt gaacctcgta caaacgcctg
    22021 cgctttctcc agacgcgttt cataatcaat acgcaacagc atcatgatgg ctgtcgacat
    22081 aatcagtaag ctcgaaccac cgtaactgat cagcggcaat gtcagacctt tggtcggtaa
    22141 catccccgcc gccgcgccaa cgttaaccag cgcctggaag ctaaaccaga tgccaataga
    22201 acaggcgaga aaaccggaaa aacggtggtc aatttctaac gctttgcggc caatcgacat
    22261 cgcgcgaaaa gcgacgaaga ataccattaa aagtgccagc accacaccga cataccccag
    22321 ttcttcgccg ataatggcga aaataaagtc agtgtgcgct tccggcagat actccagttt
    22381 ttgtaccgag ttacctaaac cttgtcccca aagttcgccg cgaccaaacg ccatcagcga
    22441 ttgcgttaac tgatagccgc tgccaaaggg atcttcccac gggttccaga atgcggtaac
    22501 acggcggata cggtacggtt cggcgagtat cagcaacaca accgctgaaa tgcccatacc
    22561 gataatggca atgaactgcc acaatttcgc tcccgccagg aacaacatcg ccagcgtagt
    22621 cacaaacaac accaccaccg taccaaggtc tggctgtgcc agcagtaaca ctgccaacac
    22681 cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta ttacgtactt cgtcgccttt
    22741 acgcaccaga tagttggcga tataacaaaa cagcgacagt tttgtcagct ccgcaggctg
    22801 gatacgcagc aaaccgagat cgatccaacg cgatgcccct ttaaccgagc tacccactac
    22861 cagcacgatc atcagcagga tgatagatcc gagcagcatc gtggcactgt agcgttgcca
    22921 gaactccatc ggcagacgca gcgtaatgat cgccagaatg aatgccagga tcagatagac
    22981 gccatcacgc ttcgcgaaga agaacggatc gttggttaag cgttgcccta tgggcattga
    23041 cgccgaggtc accatgataa agccaatcgc cgcaaggccg aaggtcagcc acagtaaggt
    23101 gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc gagcccatca cccagccttt
    23161 tagcgccgtg gagatccaga ccaggatact gaatcctggc aggcgcggca ttttcaggcg
    23221 agggagagat aaacgcatca acctaactcc ttcgccagac gggcaaactc attgcctcgt
    23281 tgttcaaagt tcttgaactg atcaaggctg gcacaggccg gggagagcag aaccatatcg
    23341 cccggctgaa cacgcggagc cagcaagcgc atcgcttgtt ccatggtttc ggtttgttct
    23401 gccacttccg ggcgtagcgc cgccagctgc gcaccgtcac gaccaaaaca gtacaaacgc
    23461 acgttgtcgc cattcagata gcgcgccagt ggactgaagt ccgccgattt accatcgccg
    23521 cccagcaaca aatgcagcgt gccgtctacc tgcaagccat tcaacgccgc ttccgtactg
    23581 ccgacgttag tggctttcga atcgttaatc cagcgtacgc cattgtgctc cagcaccact
    23641 tcaaagcgat gcggcagacc ggtgaaggtg gttaacgctt tcaggctgct ggcgcgcggc
    23701 aaaccagctg cgtctgccag tgccagtgct gccagcgcat tggtgtagtt atgctgcccg
    23761 gaaagtttca tctctttcac attcagcact ttctcgcctt taacccgcag ccaggtttct
    23821 ccctgctgat gattcaggtg atagtcaccc atgttgacgc caaagctgac gcagcgttca
    23881 tccgcaccgc gaatcggcat tgttaaggca tcatcagcat taaccacgca aactttcgcg
    23941 ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta aaccaaacgg atagcgatcc
    24001 atatgatctt cagtcacgtt cagaatggtc gctgctaccg cctgtaagct ggaggtggtt
    24061 tccagctgga agctcgacag ttccagcacg tacagttcgc actcagcatc aagcagcatc
    24121 aacgctggca ggccaatatt gccacccaca ccaacgttaa cccccgccgc tttcgccatt
    24181 tcacccacta gcgtggtgac cgtgcttttg ccgttagaac cggtaatcgc cacaatcggt
    24241 gcttgtgctt cgcgacagaa cagctcgata tcgccaacga tttcgattcc ggcatcagcg
    24301 gcagcgctta aggatggatg cgccagtgca ataccgggac tggcgacaat cagatctgcc
    24361 gccatcagcc attcatcatt cagactgccc gtgtggcgtt ctacggcttc gggtaattta
    24421 tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg gcgtcagacc gcgagcgagg
    24481 aaaaagtcca cgcaggaaag cccagtgagg cccaggccga taatgacgac atttttaccc
    24541 tgataatcag ccatgattaa cgtaccttca gcgttgccag accaatcaga accagcatca
    24601 gcgaaataat ccagaaacgc acaatgacgc gcggttccgg ccagcctttc agttcatagt
    24661 ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg cagtttaaag gagccgacct
    24721 gcaggatgac agaaagcgtt tctaccacga acacgccccc cataatcacc agcaggaatt
    24781 cctgacgtag cagtacggcg ataatgccta acgcaccacc taacgccagc gaacctacat
    24841 cgcccataaa gacctgcgcc ggataggtgt taaaccacag gaagcccagt cctgccccga
    24901 ctatcgcggt acagacaata accagttccc ctgcgtgtcg cagatacggt atatgcaagt
    24961 agctggcaaa gttcatattg ccggtcgccc acgccaccag cgcaaaacca ccggcgacaa
    25021 atacggtcgg cataattgcc aggccgtcga gaccatcggt caggtttacc gcgttgccag
    25081 tacccacaat gacgaagtaa gccagcagaa tgtagaacag ccccagctgc ggcatcacat
    25141 ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc tttgccggca aggtacaggg
    25201 cgaaggcgac acccagcgca atgaccgaca tccagaaata cttccaacga gcgatcaacc
    25261 ctttggtgtc tttacgcacc actttgcgat aatcatcaac aaagccaata acaccgtaac
    25321 ctaccagcac caccaacacg caccagacgt acggattgga cgggtaagcc cacagcagta
    25381 cggagatcac aatcgccgtc aggatcataa tcccgcccat ggtcggcgta ccgcgcttgc
    25441 tgaagtgtga ttcaggaccg tcgttacgca ccacctgacc aaaggaaagt ttttgcaaat
    25501 gagcaatcat acgcgggccc atccacaatg agatgaacag cgcggtcagc aggctgacga
    25561 tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga ataatatttg accaaatgtt
    25621 cggccagcca aactaacatg tcccattctc ctgtaaagcg cgtactacct cttccatggc
    25681 ggcactacgt gaacccttaa ctaaaatcgt aattacctgt tgctcagcaa tcagtgattt
    25741 aagacgcgta attaacgcag ttttatcagc aaaatgttcg ccaacgccgc tggcggtgct
    25801 gatagcatgg ctttgtttac ccacgcttaa cacgcggtca ataccagcag cttttgccgc
    25861 ctcgcccacc tgtacatggc aggcttcgct ttcagcgccc agttccgcca tatcgcccac
    25921 caccagcacg cggtagcccg gcatttcagc cagtacctga acagcggcgg tcattgaacc
    25981 gacattggcg ttgtaggagt cgtcgagcag caactggttt tctgccagtt ggatggggaa
    26041 cagacggcct ggaacagctt tcagatttgc cagccccgct ttgatagcat caagcgttgc
    26101 gcccacggac atggagagcg cagcggctgc cagcgcattc gcaatattgt gacgccccgg
    26161 caacggcagc agaacatcga cgctaccggt tggggtttgt agggtaaatt ccgtaccgtg
    26221 cgaggtcaca tggatattgg tggcggtgaa atcgctgttg gcggcattgg gtgagaaacg
    26281 ccacactttg cgtgagccaa ttacgctctg ccagttcagc cagtcgttgt tgtcggcgtt
    26341 cataatggcg ataccgtttt ccggcaggcc gctaaagatt tcacctttcg ctttcgcgac
    26401 acccgcaagc gagccaaaac cttccagatg cgccgctgcc aggttgttga ccagcgcagc
    26461 ttccgggcga gtcagactca cagtccaggc tatttcgccc tgatggttcg cgccaagttc
    26521 aataactgcg taatcgtatt ccggcgttaa gcgcaacagc gtcatcggta caccgatgtc
    26581 gttgttgaga ttgcctgccg tataaagcgt gttgccgcac tggcttaaaa tcgccgccgt
    26641 catctcttta acggaggttt tgccggagga ccccgtcaga gcaaccacgc gcgccggaac
    26701 ttgctggcga acccatgcag ccagttcacc aaacgccaga cgcgtatcct tgacgattaa
    26761 ctgcggcagg tcgatgtcca gcggacggct aaccagtagt gcgcctgcgc cgccagcttt
    26821 cgcctggtcg gcaaaatcgt gggcatcaaa acgttcgcct ttcagggcaa caaacaggca
    26881 gcccggcgtc agttttcggg tatcagtggt tacagcatca agggtgatat ctgcaccttg
    26941 cagttcaccg ttgagaatgt cggtaagttg gctaagggtt acgctaatca tgcaatcacc
    27001 cccagcagac gcgccaccgt gacgcgatcg gagtagtcca gacgctgatt gccaacaatc
    27061 tggtaatctt catggccttt gcccgcgacc agtaccacat cattctcttt agcctgcata
    27121 acggcgcaag tcaccgcttc agcacggcct tccatcactt tggcatgtcc ggcatctaac
    27181 attcccgcca gaatatcgtt gatgatggca cgcggttctt cggtacgcgg gttatcgtcc
    27241 gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc ccatcagtgg acgcttacct
    27301 ttatcgcgat cgccaccaca gccaaagacg caccacagct tgcccgcaca gtgcaggcgc
    27361 gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg cgtaatccac caccaccgtc
    27421 ggtttgcctg gcgcagtgaa cacttccata cgtccgcaaa ccggttgcag acgcgcggcg
    27481 gttttcagca gatcagccag tggatagccg agtgccagca gtgtcgccag cgcgagcagc
    27541 aggttgctga cgttaaaagc gcccatcaga tggctttcaa tttcgccatc gccccaactt
    27601 gagctaaagc gaatcgtcgc accgctgtcg tgatagttca cgtcgatcgc ttttaaccag
    27661 cgtccgtgac agttcggatt gatatgatct tccattgata ccgcaaccgc gtccggcagt
    27721 tttgccagcc agcggcggcc cacttcatcg tcggcgttaa taatcgcctg accgcaatga
    27781 tgttcagaat aaagcagcca tttcgcagct tcgtagtgtt ccatatcacc atgataatca
    27841 aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa atttcaatgc cgccacacgg
    27901 tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa acgtcgcgcc ctgctccacc
    27961 agccccgcca gctcatgctg aacatcgact gcggaaccgg tagtattttc tgtcgggatc
    28021 actttcccaa gcagaccgtt accaacggtg cccattaccg cgctggtttc gccaagcagt
    28081 tggctccact gcgccagcag ctgggtagtc gtggttttgc cgttggtgcc cgttacgcct
    28141 acgagacgta aattgtcaga gggttcatgg taaaagcggc ccgccagtgc agataaacgc
    28201 tcgttgagct ggctgagata gatgaccggt acgccgtgca tttcacggat ttcaccgtcg
    28261 gtcgcctcat ctttcgcctc tgcaataatg gcagccacac cttgcgctat cgcctgcggg
    28321 atatatcgac gcccgtccgc ctgatgacct actacagcta caaagagatc gcccgccgca
    28381 gccacacggc tgtcgagtgt catctctcgc agtgctcgcg aaggtgcgtc tggcacccac
    28441 ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc ccctcgcctt gattaatcac
    28501 aaattcattt ttatcgcccg ttgtcagcgc atccggctcg atgttcatgg tacgcaatac
    28561 gccgcccatg atggcaccaa agaccggcgc ggaaacggcg ccgccgtagt atttacccgc
    28621 ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc tgactcgcag gcgcaacgcc
    28681 tgcggtataa gcaatatatt tattgatgta gcgaccgtcc ggcccgacct ttttcgcggt
    28741 accggtttta atggcgatac gatagccttt aatcgccgct ttcacgccgc cgccgcctgg
    28801 tagcgccacg ctttccatca tatgcaccac ggtgcgaaca atggattccg ggaagacacg
    28861 ttcaccggga accggggggt caactttggt aatcgacagt gggcgataaa tgccgtagct
    28921 gccgatagtt gcgtagactc gcgctaactg taacggtgtt accattagcc cgtagccgaa
    28981 agagaaggtg gccctctcta tgtcagacca ccgttgtttt tgaggatata agccactgcg
    29041 ttctccgacc aaccccaaat tggtcgcttt tcccagtcca aaacgtgagt aagtatctac
    29101 taacgctgag gacggcatcg ctaacgccag cttggaaaca ccgacgttac tcgacttctg
    29161 taataccccg gtcagggtta attcgctgta gcgtgccacg tctttgattt cgtggccgtt
    29221 aattcgataa ggaatggtat tgagtaccga gttttcccgc accacgccac gttgcaacgc
    29281 ggtcattacc accatcggtt taaccgttga gcccggttca aacacgtcgg tgatggtacg
    29341 gttacgcatc gcctctttcg gcgtgccgct cagattgtta gggttgtatg acgggctgtt
    29401 agccatcgcc agcacttcac cggtgttgac atccaccagc acggcgctac cggattcagc
    29461 cttgttaaag gccaccgcgt tgttcagttc gcgataaacc agcgcctgca agcgttcatc
    29521 aatactcaac gccaggttgt gcgccgcctg gctgtcagta gaagaaatat cttcaattac
    29581 gcgaccatag cggtctttac gcacaatgcg ctcacccggc tgcccggtaa gccatttatc
    29641 gaaactcttc tcaacgccct caatcccctg actatcgacg ttagtaaagc cgatgaggtg
    29701 agcagtcact tcgccggatg gataataacg gcgagattct tcacgcagat gaatccccgg
    29761 cagtttcagt tttttgatgt agtccgccat gtcagggttt acctgacgcg ccagataaat
    29821 aaagcgccct ttcgggttgg cgttaatgcg ggcagaaagc tgatccagcg gaatattgag
    29881 cgcgttagcc agcgccttcc agcggtcacc gacgctgata ccgccagcgt catgcacttc
    29941 tttcgggtca gcccaaatcg cttttaccgg cacgctcacc gctaacgggc gaccagaacg
    30001 gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg cgaagagaac gcatgtcgcc
    30061 ctctttcacc agcatatccg gggagataac ttgtaaccac gctacgcgtc cgagcagaaa
    30121 agccagcgcc aggagaatac agccgcataa caacgcaaaa cgccaactaa taaagttggc
    30181 atgttcttcc tgacgttttg gtttctgcgt tttcgccgct gctttcatgc gtcgcgttta
    30241 tccttatttt tgcactacga tattttcttg tgacggatca acatgctgca tttgcagctt
    30301 ttccgtggcg atcctttcca cccggctatg gtcgccgagc gcattctctt caaggatcag
    30361 gttgcgccat tcaatgtcta aagcatctcg ctccagcacc agttgttcgc gctgagcggt
    30421 cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc gccgtcaaaa taatgcaaat
    30481 gaacaggcag agtggcagct tcccaaatcg caaaagatcg tcaccgataa caccaggcaa
    30541 tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg cttagagctt ctgtcactct
    30601 gctgatcatg cgttcgtcct ctctgcgata cgcagaactg aactacgggc acgaggattc
    30661 tcagccacct cttcttcgcc cggcattaac ttgcctaatg ctcgcagctg acggccaccc
    30721 agttttttga gctgctcttc agtcatcggt aaccctgccg gaacttgcgg accgcggctg
    30781 ttttcacgca taaaacgttt cacaatacgg tcttccagcg agtggaagct gatgatcgaa
    30841 agccgcccac ccggggccag cacgttgagc gagcttttta gcgcctgctc tatctcctcc
    30901 agttcactgt ttacccaaat gcgcaccgcc tggaaggtac gagtcgcggg atgtttaaac
    30961 ttatctttca ccggcgttgc agcagccacg acttccgcca gttctttggt gcgggtcatc
    31021 ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc gtttggcaaa acgctcttca
    31081 ccataggttt tcaataccca ggcgatatcg gcttcttctg cggtttgtag ccattcagcg
    31141 gctgactgcc cacgggttgg gtccatacgc atgtccagcg gaccatcgcg cataaaggaa
    31201 aagccgcgct cagcatcatc aagttgcggt gaagagacgc caagatcgag gagaatgccg
    31261 tcgatcttgc cgataagatc gcgctcggca acgtattcgc ccagcgcgga gaaaggtccg
    31321 tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa cggcgatagc ctgcgggtcg
    31381 cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg agaggatcag acgtgagtga
    31441 ccaccgcgac caaaagtccc atcaatgtag atgccatcag gacggatatt gaggccatta
    31501 acggcttcat ccagcagcac cgtagtatgt ttatagtttt ccatcatttt atagagacaa
    31561 gtcctgcagt cgctccgata agtctccggt agccaactgc tctgcgtcga tatcttcctt
    31621 gacctgttga tgccaggttg tttcatccca cagctcaaac ttgttgaact gtccaaccag
    31681 catcacttct tttgtcagcc cggcatgttg ccgcagtact ggcgcgatta acaatcgacc
    31741 tgcgccatcc atctgacatt cgctggcatg acctaacagt aggcgctgca cacggcgctc
    31801 aaccgggttc atgctcgaca gacgcgataa tttttgctcg ataatttccc attcaggcag
    31861 ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg caaaccattt gaccggcagc
    31921 gttctcaagc agctgttccc ggtaacgggt aggcactgat aagcgccctt tgctgtcgag
    31981 attgactaac gttgctcccc ggaacatgcc agcctcaccc cttattccca ctttgcccca
    32041 caatttccca ctgaaaggag tttacggagc ggaggaaaag cttgtcaagc cagtcacaag
    32101 gcataccgag gtaaaaaacc cgatgtttac ggctaatatc agcgttgttt attttacgtc
    32161 caacgtgcgc agcaaattaa cgttatgaat agctgtaaga aaaaatccct catcgtcatc
    32221 accgttttaa aatgactcac tattttccac agcaaatata aagtgtcagt ttgcgacgcg
    32281 agcggcattt taggacatat cttccccggt taacagtccc tgttgcgtct gtgtggcgct
    32341 tgatgccagc gggtttgcgc aaaagcaaag aaaagtgctt gttaaatcgg caattcatct
    32401 ctgcatgtaa caaaataatg caaaaacgcg ctgccgatat cacttaatta ttgtgaattt
    32461 cagctggtga gttaattaac gggcgggttt aaaagaactt atcaataata gtcaggaata
    32521 gtcttattta ctttaaggat attgatgtcc attcccgtac tctacgcgcc agagggaaat
    32581 tcacctggcg cgtatttttg ttcgcggctt agctacggct gagcacaccg cggcgataga
    32641 gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg cggttcgtcc aggcttgcca
    32701 ggacaatctc cagcacacgc tctgcgacat cgcggtgacg ttgagccact gccagcaccg
    32761 ggcactgtaa gaagtcgagc agttcgttat cgccaaaggt ggcaattgcc aggtcagaag
    32821 gcagtttacc atcgcgacgt agcgtgacat ccatcacccc ttgcaacaac gcaaacgacg
    32881 ttgtgaacag cgcctgcggc atcggatgcg tttccagcca tttttcgaat aactgggcag
    32941 ccgcctcccg ctcatagctg ttggcataca ggaaatgcac ttcgcgcgga tcatctttcc
    33001 aggcagtacg gaaaccttgt tcacgcagga agctgacaga aagctccggc agcgcgccaa
    33061 gataaagcac cgtctcggcg ggaaacttac gtaactcttc cgccagcatt tcggcatcat
    33121 cctgatcggc accaaccacg ctggtgaaat gttcacgatc gagagcgcgg tccagcgcga
    33181 caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg atgctcagga ggcaacgacg
    33241 tcgaaacaat aatggcatcg acctgacgct gtaaaaggtg ctcaatacag cgcatttcgt
    33301 tgtctggctg atcttctgag caggcaatta gcagttgata accccgttgc cgcgcctggc
    33361 gttcaagata gttagcgatg cgggtatagc tggtgttctc cagatcgggg atcacaagac
    33421 caatagaacg tgtgcgtcca gcacgaagcc cagctgccac ggcgttcggg tggtaattgt
    33481 gctcacgcac cacagccatg actttttcaa cggttttgtc gctcacacgg tattgcttcg
    33541 ctttgccgtt aataacatag cttgcagtgg tccgcgacac tcccgccagc cgagcgattt
    33601 catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa ccatagccat tgtactggta
    33661 tgggttaaat tcattaacat ctaagcgcag aaaattaact gcggcaactg cttttattcc
    33721 aggttcccac tgatttcgca aaaaaagccc aacgtgacat gttgggctgc aaattgcgca
    33781 ctgagatcat tctcaacgca ttattttatc gccgcgcgaa agtccgacca caccagagcg
    33841 agcaacttcc acaattttcg ccacatcgcg aatcgatgct aaaaatgcat caagcttatc
    33901 gctggtgcct gctaattgaa cggtataaag cgagggggtg acatcgataa tttgcccacg
    33961 gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa ccgctggcct gaattttcac
    34021 cagcatgatt tcccgctcaa catgcgcgcc ctgccccaac tcactcacgc gcaagacatc
    34081 gaccagcttg tgtaattgct tttcgatctg ctcaagcact ttttcatcgc ccacggtctg
    34141 gatggtcata cgcgataatg tcggatcgtc ggttggcgca acggtcaggc tttcaatgtt
    34201 gtagccacgc tgggaaaaaa ggccaatcac gcgggataac gcgcctgatt cattttcgag
    34261 taagactgat aatatccggc gcataatcag gttctctccg ttttgcttaa ccacatttca
    34321 tccattccgc ccccgcgaat ctgcatcggg tagacgtgct cactgccatc gacggtaaca
    34381 tcaacaaaca ccaggcgatt attgcgcacc tgttccagcg cctcgctaag tttgctttcc
    34441 agctcatgcg gatgagagat ctggatcccg acgtgaccat aggcttccgc cagacggacg
    34501 aaatcgggta gtgattgcat ataagattgt gaatgacggc cggaatagat catgtcctgc
    34561 cactgtttca ccatccccag atagcggtta ttgagattca ccaccagtac cggcaactcg
    34621 tattgcaacg cggtagacag ttcctggatg ttcatctgaa tactgccgtc gccagtgacg
    34681 caaaccacgg tttcttctgg caacgccatt ttgacgccca gtgccgcagg taaaccaaaa
    34741 cccatcgtgc cgaggccacc ggaattgatc cagcgacgcg gtttgtcgaa tggataataa
    34801 agtgcagcaa acatctggtg ctgcccgaca tcggacgtca cgtaagcgtc tcccttcgtc
    34861 aaccgccaaa gagtctcgat caccgcctgc ggtttaatct tttcactgtg agtgtcatat
    34921 ttcaggcact gacgagcgcg ccactgttca atttgctgcc accagtcgcg gatctcatcc
    34981 agtggttgat gggcggattc ttgcgacaag agttcaagca tttgttcgag gacctggcga
    35041 gcatccccca caatcgggat atccgcagtc acggttttag aaatggaagt aggatcaata
    35101 tcgatatgca gaacagtggc atttgggcag tactttgcca gattgttcgt cgttcggtca
    35161 tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat gcatcgtcat attggcttcg
    35221 taggtaccgt gcattcccag catgcccagt gcctgacgat gcgttgccgg aaacgccccc
    35281 agccccatca atgaggaaac aacgggcaga ttcaacgcct ccaccgtttc tttcaactgc
    35341 tgatggcagc ccgccatgat tgccccaccg cctacgtaga caaccggttt ttttgccgcc
    35401 accagcgttt gcagagcacg cttaatttgc cctttatgtc cggtagtagt gggattgtaa
    35461 gaacgcatac taaccgactc cggccagaca tagggtaatt tgttcgccgg attaagaata
    35521 tctttcggta aatcaacgac tactggtcca ggacgaccac ttgccgccag ccagaaagcc
    35581 tttttcagca cctgcggaat gtcttccgtt tgcttaacca gaaaactgtg tttaaccacc
    35641 ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat cgtaacctat caacgaggtc
    35701 gctacctgcc cggaaaggac aactaatgga atggaatcca tataagcggt ggcgatgccg
    35761 gtaatcgcat tggtcgcccc tggacccgac gttaccagca cgacgccgac ttccccggtc
    35821 gcacgcgcca ggccatcggc catatgcacc gccgcctgct catgacgaac taatacatga
    35881 tcaataccac ccacggtatg caatgcatcg taaatatcaa ggactgcgcc tccgggataa
    35941 ccgaatactt gtttaacgcc ctgatcgata agcgatcgga cgaccatctc ggctccagac
    36001 aacatctcca tggcctgcct cactgtttga cggaaaaaat gtgtaaaaca cattaaccgt
    36061 ccgatcttgc ttaagcaatt ggcagccaga gacataaacg cagggtaata agaataaaca
    36121 ggaggaaagg tgaaaagaaa cgataaaatc ctccattacg aaggattaaa ttgacaataa
    36181 atcggctga
//
LOCUS       DAPPNJ010000022        35351 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000022 DAPPNJ010000000
VERSION     DAPPNJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.022"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            115..528
                     /locus_tag="RPM73_000842"
     CDS             115..528
                     /locus_tag="RPM73_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDX6519233.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="RPM73_000843"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="RPM73_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDX6519234.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="RPM73_000844"
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="RPM73_000844"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDX6519235.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="RPM73_000845"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="RPM73_000845"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDX6519236.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="RPM73_000846"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="RPM73_000846"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDX6519237.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="RPM73_000847"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="RPM73_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDX6519238.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="RPM73_000848"
     tRNA            complement(3794..3881)
                     /locus_tag="RPM73_000848"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
     gene            4308..5426
                     /gene="hyaA"
                     /locus_tag="RPM73_000849"
     CDS             4308..5426
                     /gene="hyaA"
                     /locus_tag="RPM73_000849"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDX6519239.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5423..7216
                     /gene="hyaB"
                     /locus_tag="RPM73_000850"
     CDS             5423..7216
                     /gene="hyaB"
                     /locus_tag="RPM73_000850"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDX6519240.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7235..7942
                     /gene="hyaC"
                     /locus_tag="RPM73_000851"
     CDS             7235..7942
                     /gene="hyaC"
                     /locus_tag="RPM73_000851"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDX6519241.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7939..8526
                     /locus_tag="RPM73_000852"
     CDS             7939..8526
                     /locus_tag="RPM73_000852"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDX6519242.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            8523..8921
                     /gene="hyaE"
                     /locus_tag="RPM73_000853"
     CDS             8523..8921
                     /gene="hyaE"
                     /locus_tag="RPM73_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDX6519243.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8918..9775
                     /locus_tag="RPM73_000854"
     CDS             8918..9775
                     /locus_tag="RPM73_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDX6519244.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            9909..11453
                     /gene="appC"
                     /locus_tag="RPM73_000855"
     CDS             9909..11453
                     /gene="appC"
                     /locus_tag="RPM73_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDX6519245.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11465..12601
                     /gene="appB"
                     /locus_tag="RPM73_000856"
     CDS             11465..12601
                     /gene="appB"
                     /locus_tag="RPM73_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDX6519246.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAVLLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12614..12706
                     /gene="cbdX"
                     /locus_tag="RPM73_000857"
     CDS             12614..12706
                     /gene="cbdX"
                     /locus_tag="RPM73_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDX6519247.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12786..14084
                     /gene="appA"
                     /locus_tag="RPM73_000858"
     CDS             12786..14084
                     /gene="appA"
                     /locus_tag="RPM73_000858"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDX6519248.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(14199..16379)
                     /gene="etk"
                     /locus_tag="RPM73_000859"
     CDS             complement(14199..16379)
                     /gene="etk"
                     /locus_tag="RPM73_000859"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDX6519249.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(16399..16845)
                     /gene="etp"
                     /locus_tag="RPM73_000860"
                     /gene_synonym="yccY"
     CDS             complement(16399..16845)
                     /gene="etp"
                     /locus_tag="RPM73_000860"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDX6519250.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(16833..17972)
                     /locus_tag="RPM73_000861"
     CDS             complement(16833..17972)
                     /locus_tag="RPM73_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDX6519251.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(18018..20114)
                     /locus_tag="RPM73_000862"
     CDS             complement(18018..20114)
                     /locus_tag="RPM73_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDX6519252.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(20114..20860)
                     /locus_tag="RPM73_000863"
     CDS             complement(20114..20860)
                     /locus_tag="RPM73_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDX6519253.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(20857..21501)
                     /gene="gfcB"
                     /locus_tag="RPM73_000864"
     CDS             complement(20857..21501)
                     /gene="gfcB"
                     /locus_tag="RPM73_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDX6519254.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(21608..21913)
                     /locus_tag="RPM73_000865"
     CDS             complement(21608..21913)
                     /locus_tag="RPM73_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HDX6519255.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(22355..22567)
                     /gene="cspH"
                     /locus_tag="RPM73_000866"
     CDS             complement(22355..22567)
                     /gene="cspH"
                     /locus_tag="RPM73_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDX6519256.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            22853..23065
                     /gene="cspG"
                     /locus_tag="RPM73_000867"
     CDS             22853..23065
                     /gene="cspG"
                     /locus_tag="RPM73_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDX6519257.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            23076..23264
                     /locus_tag="RPM73_000868"
     CDS             23076..23264
                     /locus_tag="RPM73_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDX6519258.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            23239..23469
                     /gene="ymcE"
                     /locus_tag="RPM73_000869"
     CDS             23239..23469
                     /gene="ymcE"
                     /locus_tag="RPM73_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDX6519259.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            23459..23632
                     /locus_tag="RPM73_000870"
     CDS             23459..23632
                     /locus_tag="RPM73_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDX6519260.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(23680..24753)
                     /locus_tag="RPM73_000871"
     CDS             complement(23680..24753)
                     /locus_tag="RPM73_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDX6519261.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVSSTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(24825..27569)
                     /gene="torS"
                     /locus_tag="RPM73_000872"
     CDS             complement(24825..27569)
                     /gene="torS"
                     /locus_tag="RPM73_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDX6519262.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            27652..28680
                     /gene="torT"
                     /locus_tag="RPM73_000873"
     CDS             27652..28680
                     /gene="torT"
                     /locus_tag="RPM73_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDX6519263.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(28653..29345)
                     /gene="torR"
                     /locus_tag="RPM73_000874"
     CDS             complement(28653..29345)
                     /gene="torR"
                     /locus_tag="RPM73_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDX6519264.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            29475..30647
                     /gene="torC"
                     /locus_tag="RPM73_000875"
     CDS             29475..30647
                     /gene="torC"
                     /locus_tag="RPM73_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDX6519265.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            30647..33193
                     /gene="torA"
                     /locus_tag="RPM73_000876"
     CDS             30647..33193
                     /gene="torA"
                     /locus_tag="RPM73_000876"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDX6519266.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYNKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            33190..33789
                     /gene="torD"
                     /locus_tag="RPM73_000877"
     CDS             33190..33789
                     /gene="torD"
                     /locus_tag="RPM73_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDX6519267.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(33882..34187)
                     /gene="cbpM"
                     /locus_tag="RPM73_000878"
     CDS             complement(33882..34187)
                     /gene="cbpM"
                     /locus_tag="RPM73_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDX6519268.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(34187..35107)
                     /gene="cbpA"
                     /locus_tag="RPM73_000879"
     CDS             complement(34187..35107)
                     /gene="cbpA"
                     /locus_tag="RPM73_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDX6519269.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         8429 a         9072 c         9283 g         8567 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttaaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gattagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacca cgtgatacca cggagcggcg cgaagctcgt cattcaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc gggtaggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc ccgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 tgggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctat cacgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagtttca tgccgtgttc ttcaatcggc agcagggcag gtggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gcgccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agccagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg tgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtatgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtgga acgggtgctt
     2461 agcgagccgc atcatccctc gggggaatta gccgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cgatataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttctggcga cagcgaaatc ccttcgttct ctgcaatcac cacagccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggcta
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtctcaccg ccatgaatga tgttgctggt ttcaaacaag
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagt
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gccaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     4141 acgctttatt aacaatactt tctggcgacg tgtgccagtg cagaaggatg agctttcgtt
     4201 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     4261 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     4321 aaacatttta ccaggccatg cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt
     4381 gtagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     4441 cgctggagaa caaaccccgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     4501 gtaccgaatc ttttatccgc tccgctcacc cactggcgaa ggacgtcatc ctttccctga
     4561 tttccctcga ttatgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     4621 tcgaagacat catcacgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     4681 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     4741 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     4801 gcgtgcaggc cgcgcggccc aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     4861 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     4921 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccgctga
     4981 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     5041 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg
     5101 gctgcaaagg gcctaccacc tataacgcct gttcctccac acgctggaat gatggcgttt
     5161 ctttcccaat ccagtctggt cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc
     5221 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     5281 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     5341 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaacc gaacatcagc
     5401 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     5461 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg
     5521 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     5581 tcgcgggctg gagatcattc tgcaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     5641 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     5701 tgctatcggt attaaagtgc cggacaacgc caatatcatc cgcaacatta tgctggcaac
     5761 gctctggtgc cacgatcatc tggtgcactt ctatcagctt gccgggatgg actggatcga
     5821 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     5881 ctcttggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     5941 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     6001 actgccgcca gaagctaacc tgatgggctt tgcccactat ctcgaagctc tcgatttcca
     6061 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     6121 tgtcggcggg atgccttgcg ccatcaacat tgacgaaagc ggcgcggtcg gggcagtcaa
     6181 tatggaacgc ctgaacctgg tgcagtcaat tatcacccgc acggcggact tcattaacaa
     6241 cgtgatgatc cccgacgcct tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg
     6301 cactggtctt tctgataaat gcgttctcag ctacggcgca ttcccggata ttgccaacga
     6361 ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     6421 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     6481 gtatcgatat cccaacgatc aggtcgggcg tcatccgttc gatggcatca ccgacccgtg
     6541 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     6601 acgctactcg tggatcaaag cgccacgctg gcgcggtaac gcgatggaag tggggccgct
     6661 ggcgcgcacg ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     6721 catgatgtcg gcgttgaacc tgccgctttc cggtatccag tcaacgttag gccgcatttt
     6781 gtgccgcgcg cacgaagcgc agtgggccgc aggtaagttg cagtatttct tcgacaagct
     6841 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     6901 aacctggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     6961 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     7021 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cggcgctgat
     7081 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     7141 tgacccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     7201 cgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     7261 cagccactat gtctttgaag cgccagtccg catctggcac tggttgacgg tgttatgcat
     7321 ggcggtgttg atggtcaccg gatactttat cggcaagccg ctaccttccg tcagcggcga
     7381 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatggt
     7441 ttttaccgtg gttttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     7501 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     7561 ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat ataggccata atcccatcgc
     7621 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggttttgc
     7681 gctgtacagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     7741 tttctactgg acgggtggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     7801 gctgattggc gcgtttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     7861 cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat ttggcaaaat
     7921 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg
     7981 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     8041 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     8101 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     8161 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     8221 ctgcatcaga acagtttctc cgaagtgctg gcgctggcag atatccgcgg acatctgcca
     8281 gcacatattg ccctcgtcgg tctgcaaccc gctatgctcg acgactacgg cggcagcctg
     8341 agcgaactgg cacgggatca actgcccgct gcggaacaat cggcgttggc gcaactggct
     8401 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     8461 tcgatggaaa attacgaagg cgttcgtttg cgccagtaca ggatgacact ggaggagcag
     8521 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     8581 cgccggtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtggtgt
     8641 tattaagcag tgacccgaaa cgtacgccag aggtcagcga taatccggta atgattggcg
     8701 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     8761 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc cgccacatta gtgtttaccg
     8821 gcggaaacta tcgcggtgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     8881 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaactt ttttccatct
     8941 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     9001 ctgtcaggtg aatgatgaac cgagtatggc ggccctggaa caatgtgctc acagcccgca
     9061 ggtgattgcg ctgttaaacg agttacaaca tcaaatgagt caacgccagc cgccgttggg
     9121 cgaggtgctg gcagtcgatc tgttaaatct ccacgccgac gatcgtcact ttatcaatac
     9181 ccttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca gtgaaagtga
     9241 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     9301 gctggaggac aaactggaag ctggctgtgc gccgctggcg ttgtggcagg cggcaacgca
     9361 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     9421 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     9481 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     9541 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     9601 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggccctt tactggaaag
     9661 ttatgaaatc tgcccgatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     9721 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     9781 cggtaagtat cgctttcagt cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     9841 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     9901 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg accgcgctgt
     9961 atcacttttt atttgtaccc cttaccctgg ggctgatttt tttgctggct atcatggaaa
    10021 ccatttacgt ggtcaccggc aaaacaatct accgcgatat gacgcgcttc tggggtaagc
    10081 tcttcggtat caattttgct cttggcgtgg ctaccggcct gaccatggag tttcagtttg
    10141 gtactaactg gtcattctat tccaactatg tgggcgatat ttttggcgca ccactggcga
    10201 tggaagcatt aatggccttc ttcctcgaat ccacctttgt cgggctgttc ttcttcggct
    10261 ggcaacggct gaataaatac cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa
    10321 atctctctgc gttgtggata ttgaatgcca acggttggat gcaatacccg accggtgcgc
    10381 attttgatat cgacaccctg cgtatggaga tgaccagctt cagcgagctg gtctttaacc
    10441 cggtcagcca ggtgaaattt gtgcacaccg taatggcggg ctacgtgacc ggggccatgt
    10501 ttattatggc gatcagcgcc tggtatttac tgcgcggacg ggagcgcgat gtcgcattac
    10561 gttcgtttgc catcggttcc gtcttcggta ctctggcgat tatcggtacc ctgcaactcg
    10621 gagacagttc tgcgtatgaa gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag
    10681 gggagtggca aacggaacct gcacctgcac cgttccatgt ggttgcctgg ccggaacagg
    10741 atcaagagcg taacgccttt gccatcaaaa ttcccgcgct gctagggatc ctcgccactc
    10801 actcattaga taaacccgtg ccgggtctga agaatttgat ggctgaaacc tacccacgct
    10861 tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc
    10921 cgcatgtgtt gcaggcattc cgggaactgg aaggtgacct gggctacggc atgttgctct
    10981 cccgctatgc gccggatatg aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg
    11041 gcgcgatacc tcaggttgcg ccggtattct ggagtttccg catcatggtg ggctgtggtt
    11101 ccctgctgct actggtgatg ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc
    11161 agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc gttgccgtgg attgcgattg
    11221 aagccgggtg gtttatgacc gagtttggtc gtcagccgtg ggcgatacag gacatcttac
    11281 cgacatactc cgcgcactcc gctttaacca caggacaact ggctttctca ctgatcatga
    11341 tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc
    11401 gtctggggcc gagcgcgatg cagagtgaac aaccgacgca gcaacagggg taaaggagaa
    11461 aatcatgttt gattatgaaa cattgcgctt catctggtgg ctgctgattg gcgtgatcct
    11521 ggtggtcttt atgatctccg acggatttga catggggatc ggctgtctgc tgccgctggt
    11581 ggcgcgtaat gatgatgaac gccggatagt gataaacagc gttggtgcac actgggaagg
    11641 caaccaggtc tggttgatcc tcgctggtgg ggcattattt gccgcctggc ccagagtgta
    11701 tgcagcggcg ttttccggct tttatgtggc gatgatcctg gtgctgtgct cgctgttctt
    11761 ccgtccgctg gcctttgatt atcgcggaaa aattgccgat gcacgctggc gtaaaatgtg
    11821 ggatgccggt ctggtcatcg gcagtctggt gccgccggta gtcttcggta tcgccttcgg
    11881 caacttgttg ctcggcgtgc cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg
    11941 cagcttctgg caactgctga cgccattccc tttattgtgc ggattgctca gccttgggat
    12001 ggtgattttg caaggtggcg tctggttaca actgaaaact gttggtgtga ttcatctgcg
    12061 ttcacagctg gcgactaaac gcgctgtact gttggtgatg ctgtgctttt tgctggcggg
    12121 ttactggctg tgggtcggta ttgatggctt tgtactgctc gcccaggatg ctaacggtcc
    12181 ttccaatccg ttaatgaaac tggtggcagt gctacctggt gcctggatga ataatttcgt
    12241 cgaatcgccc gttttgtgga tcttcccgct gctggggttc ttttgcccat tgctgacggt
    12301 gatggcgatt tatcgtggtc gcccggggtg gggatttttg atggcgtcat tgatgcaatt
    12361 tggcgtgatt ttcacggcag gcatcacgct gttccccttc gtcatgccgt caagcgtgag
    12421 tccgatctcc agcctgacgt tgtgggacag cacttccagc cagctgacgc tgagtattat
    12481 gttggtaatc gtgctgatat ttttgcccat tgtgttgctc tacactctct ggagctacta
    12541 caaaatgtgg gggcgcatga caacagaaac tctccgccgt aacgaaaacg agttgtacta
    12601 aggagcagga acaatgtggt atttactttg gttcgtcggc attttgttga tgtgttcgct
    12661 ctccaccctt gtgttggtat ggctggaccc gcgtctgaaa agttaacgaa cgtaggcctg
    12721 atgcggcgca ttagcatcgc atcaggcaat caataatgtc agatatgaaa agcggaaaca
    12781 tatcgatgaa agcgatctta atcccatttt tatctcttct gattccgtta accccgcaat
    12841 ctgcattcgc tcagagtgag ccggagctga agctggaaag tgtggtgatt gtcagtcgtc
    12901 atggtgtgcg tgctccaacc aaggccacgc aactgatgca ggatgtcacc ccagacgcat
    12961 ggccaacctg gccggtaaaa ctgggttggc tgacaccgcg cggtggtgag ctaatcgcct
    13021 atctcggaca ttaccaacgc cagcgtctgg tagccgacgg attgctggcg aaaaagggct
    13081 gcccgcagtc tggtcaggtc gcgattattg ctgatgtcga cgagcgtacc cgtaaaacag
    13141 gcgaagcctt cgccgccggg ctggcacctg actgtgcaat aaccgtacat acccaggcag
    13201 atacgtccag tcccgatccg ttatttaatc ctctaaaaac tggcgtttgc caactggata
    13261 atgcgaacgt gactgacgcg atcctcagca gggcaggagg gtcaattgct gactttaccg
    13321 ggcatcggca aacggcgttt cgcgaactgg aacgggtgct taattttccg caatcaaact
    13381 tgtgccttaa acgtgagaaa caggacgaaa gctgttcatt aacgcaggca ttaccatcgg
    13441 aactcaaggt gagcgccgac aatgtctcat taaccggtgc ggtaagcctc gcatcaatgc
    13501 tgacggagat atttctcctg caacaagcac agggaatgcc ggagccgggg tggggaagga
    13561 tcaccgattc acaccagtgg aacaccttgc taagtttgca taacgcgcaa ttttatttgc
    13621 tacaacgcac gccagaggtt gcccgcagcc gcgccacccc gttattggat ttgatcatgg
    13681 cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg tgtgacatta cccacttcag
    13741 tactgtttat tgccggacac gatactaatc tggcaaatct cggcggcgca ctggagctca
    13801 actggacgct tcccggtcag ccggataaca cgccgccagg tggtgaactg gtgtttgaac
    13861 gctggcgtcg gctaagcgat aacagccagt ggattcaggt ttcgctggtc ttccagactt
    13921 tacagcagat gcgtgataaa acgccgctgt cattaaatac gccgcccgga gaggtgaaac
    13981 tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat gtgttcgttg gcaggtttta
    14041 cgcaaatcgt gaatgaagca cgcataccgg cgtgcagttt gtaatgcata aaaaagagca
    14101 ttcagttacc tgaatgctct gaggctgatg acaaacgaag aactgtctaa tgcgtagacc
    14161 ggaaaaggcg ttcacgccgc atccggccac tttcagtttt actctttctc ggagtaacta
    14221 taaccgtaat agttatagcc gtaactgtaa gcggtgctgg cgcgtttaat cacaccattg
    14281 aggatagcgc ctttaatatt gacgcctgcc tgttccagac gctgcattga caaactcacc
    14341 tctttggcgg tgttcaagcc aaaacgcgca accagcaggc tggtgccaac agaacgcccc
    14401 acgaccgcgg catcactcac cgccagcatc ggcggcgtat cgacaatcac cagatcgtaa
    14461 tggtcgttcg cccattccag taattgacgc atccgatcgc gcatcagcag ttcagacggg
    14521 ttaggtggca cctgaccgcg agtaatcaca tcaaagcctc ctttgccaaa atgctggatc
    14581 actttgttga gctcatcttt acctgccaga tattccgaca agccatgttc attactcacg
    14641 gtaaacaggt tatgcgaata accacggcgt aagtcggcat caataaataa cactttttga
    14701 tcggactggg cgatcaccgc tgccagagtt gaactgacaa acgttttacc actgtctggc
    14761 gtcgcaccgg tgatcatcag aatgttattc tccgtctcca tcatagcgaa atgcagactg
    14821 gttcgtagcg cacgtacggc ttccacagca gaatccgccg ggttatccac cgccaggaag
    14881 gggatatttt tagtacgatg gcgctgctga ttagaaaata aatttttctt acgcagacgg
    14941 gtgcgtttat ccagccactc ggacattggg atagtggcat aaacgctgat gccgtgctct
    15001 tccagttgtt ccggggcttc tacaccacga cgcaacatcg cacgcgccag cacggcaccc
    15061 acagaaataa acaggccaag aataaaacca agcaccacat tcaacgcttt tttcggtttc
    15121 actggctgcg gctgagtgac tgccgggtcg ataatccgca cgttaccaat ggcactggat
    15181 ttcgaaatac tcaactcctg ctggcggtta agtaattgca gatataccgc acggcccgct
    15241 tctacgtcac gacttaaacg caacacttcc tgttgggtgg aaggcattgc cgatacccgc
    15301 ttattcaggc gtttgcgttc ttgctccagc gtctggcgtt tttccagcag cgcacgataa
    15361 gttgggtgat ctttcttata cagctgggag atctctgcct cgcggaaggt cagctcattg
    15421 agttgattat caacgttcac aatctgctca agaacggctt tggcttccag gttaaggtca
    15481 accgaatcgc gctgctggcg ataaacgttg agtttttctt ccgcctggtc cagctcgctg
    15541 cgcacttcag gtaactggcg ctgtaagaat tcaaggcttt gtgaatcctg cgccgcctgg
    15601 cgagcgatat tctgttgcaa atagttgtta gcgatgctgt tcagaatacg agtaatcaac
    15661 tggggatcat caccagtcat ggtaagttcc agcatcccgc tttctttact gcgttcgcta
    15721 acggtaaagg tttcctgcaa tgcgttaatc gcttccagtt cggtacgctg gctcaggaca
    15781 aactgtgttc ctggtttggc cttaatgtcc gcgatagtca gcgcaacgcc atctttttcc
    15841 agacgctggc cgaccatacc attgacggtg aactcttcac cttccagtgt atagtggccg
    15901 ttttccccaa ccgtgagtgt cagttgctga tcctgaccat tcagttgtgg aatatgcatc
    15961 cagctgatcg ccagctcacc tggtttttct ttggttaatc tcgcccagcc gcgacccaca
    16021 atcggaaaat acttctgctc aactatgtcg cgcagattca gttcagcaat ggttttaccg
    16081 agaatcatgc gcgattgcag cagttggatc tccggtgcag actcgggcga tgagttaggg
    16141 atcatatcgc tcaggccgct gagaatggcg ttgccctgtt tttgctcaac ctggaccaga
    16201 gtatctgcct gataaattgg tgtacttaac agcgagtaag cgacagcgat cagcgtgaat
    16261 aacgcggtca cgctgataat aaacttacgg tgatcccata actcgccgac cagacgaagc
    16321 agatcgatct cattttcctg agtgctgcct ggtggcgtat tcatattttt agttgtcatc
    16381 gtattttata aactcaaatt accggctgag gcgcttcgcc cattcctgac tggcgcgctc
    16441 caacataccg tagacatgtt caaatgcgtc ctgactttta cgatagggat ccgggatctc
    16501 tttctgttcc agccattgcc caaacagcat tgtcttcccg cggacctcgg gagcgattgc
    16561 ggtaacctga gcaatatgct ctgactccat tgccagaatc agatcgtaat ttcgtgccat
    16621 ctcagcagtg agcttacgtc cggcatgtcc ttcaagagat acgccatgat tagcggcaac
    16681 atctgctgct gtcgcatctg cagggtgttt taccagacca tgaacacctg ccgatttgac
    16741 ttttacgcct ggcagacgtt tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt
    16801 gccggtacaa accaccagga ttgagttaaa ttttagttgg gccatgtttg aatccaccgt
    16861 gaagtctctg tcaggtcatg caccccggaa atcgtcggta ccagcagaga aatcacacgg
    16921 ttccaacgag ccagcggcgc agtagtgaca taaacaatgt cgtaaggttc aagctggaat
    16981 tccgttccga gtatcattgc cgatgcgtcc ttcgcattca gctgataaat attggcgatt
    17041 ttgccattct gcttgttttg cgttgcacga ataacaaaaa tccccgtcgc atcagcgaca
    17101 tcctgattca taccctcagc gttacccagc gcttccgcca gggtcatacc gctgcggtcc
    17161 attttcagcg tgctctgttt acccacttca cccatcacga agactttcag atcatcgttg
    17221 cgcggaataa ataaaatatc gcccgggtgc agcagtttgt tctggcgcaa gtcgccacgc
    17281 tgcatcagcg catagaggtt cactttggtc tttacgccat tctgggtgag caccacgttg
    17341 cgccagtctg catctgcggt cagaccgccc gcggcgttaa tcgcatccat gatggtcaga
    17401 ggaatattgg taatcggctg ctgacccgat ttgctgactt cgccggtgac gtacgctttt
    17461 tgcgagcgga acgccgcgac gctgacatcg acttgcgggc tttcaatgac tgaatccaga
    17521 cgcgctgtaa tctcgttgcg tacctgggta agtgttttcc cggccacttt caggcgacca
    17581 atataggggt agaaaatcgc accatcggcg ttaacccagt tgccggtatc actggcgcta
    17641 cggtattgcc ctgcgggggt ggttagttcc gggtgatccc agaccgtaac catcaacaca
    17701 tccccaatgc cgatacgata ttcatagttt gcgatttgct gctccagctc cgggttagcc
    17761 tgcgacataa tcggtttggc gcgcaactga tcaatcaatc ctggcgtgac gggatagata
    17821 ttgaccattt tgtccaggtc atactgattg tcaggtagtt caatgacatc cttgttactg
    17881 gtcgataaat tctgaccagg aacaagggta catgctgtca aagacagaac agccaatgtc
    17941 agtaccgaaa acttaaaaat gttctttttc attggtttat atttatcagt caatgtaatg
    18001 ttatgaatta cgtcaggtta ttgtccaacc ggtacttctc gctctgccgt cattggatag
    18061 agctggtatt tgcgtgacag catttgtcca ccatcacgcg tcaatggtgt ccacgaaacc
    18121 accgcgcggt tgcggttagg tccaatggtc atcaagtcga atggaatcga gatataaaaa
    18181 cctttactaa agccgccttc gccgtagtca tctttagata cgttactgat tgccgcccat
    18241 acccctaccg ccacgccgct gtcaaagcgt ttggcgacgt cgatcgttgc gcctttatct
    18301 tttgccagat attgcccaac gctaagtttc atcagtacgc cattgagcgt cggcgggttc
    18361 cagtaagccg tcacgaaacc tgttggcgtg gaataatcgg tgaaacgcat catgttgtcc
    18421 cagtcacgtt gcttcacgta gttaacgtcc acacccagcg cccagctggc atctagcggg
    18481 cgataaagca gctcggaacc gacaccggcg tacatcgttt ccagataacc gccatacacc
    18541 tggccataga aaccattgcc taagtcggca aagtagttcg cctgcaagtt gttgagataa
    18601 acgtcattgc gaacgtaatc acggatatgc gtgcgcacgc ggggcagggt agagtccgcg
    18661 ggcaacagcg aagacttaaa cttgtcgtag ttgttgtaaa tattggtgaa aataccgcca
    18721 tcaagcagca ggtggtcggt aaaccagtag cgggcactgg acatcagccc cagctggaac
    18781 atatagaaat cttccgggcc gcccagcgac tgtgaaagtg ttgggttgaa agagtagcta
    18841 aagcgatctt cacgaatacg gtagccccga ccaaaggcag aaagatcttc tgcttcaaca
    18901 cgttgttgtt gaagttgctc tgattgacca ggcgctgtac cagccagctg tttgcgcagg
    18961 ctggctacgt cggtttcggt agtcaccatc gccatatgct cgcgcttttg cgtcacgcta
    19021 atcttctcaa cgccttgcgg caggttattc accagaatcc ggttggcacg atcgaccgct
    19081 tcgcgagaat cacggtattt gtattgttga ccagacatat acagcgtctt atcgcgcagc
    19141 tgaatttccg gtgcttcaaa acctgcgttg tacttcagcg cggtaagttg gttcgctact
    19201 gtggtgtact gcaatccttc agattcaggc gcaggttgat atgccggttt tggcgtatcg
    19261 cgcagggcag ggcgcagatc gttgaaattg gtccgtaacg tgaagccaaa catcaacgtg
    19321 ttaccgcgtt cataactcag gttgagatct gcccagctgg cagcgcgata aactgcgccg
    19381 acgttgaaat ggcttgcctg aggcagtttg ccagcgaaat cattctggta attgttgcca
    19441 tcgtattcga gtttcagacg caggggattc cacggcgttt gatactcaat gccgccaaag
    19501 atggaagccg ggccacgaaa gatatcgcta aagctgatat cgcccgcatc gtgagactct
    19561 gcgcgatgac aatatttatc gctcacccgg caaaacgggt tggtaatatt gcccgcatta
    19621 ccggcgtagc cccatgccat cccgagggtg aaatcaaatg gccccgcttg cttgctggcc
    19681 accagatact caccgtcaaa cagacccgta ccagcaatat cacgtttacc aaacgccact
    19741 tgcggtagcc agtaaccttc ttcccacagg cgaagtttaa aatcgaatga tttgtctttg
    19801 tatgactgat cgccgctgaa atcttcccac tggctatatt tgcgtgtgcg cacatccgta
    19861 taacgaatgg tgccttccag ccatgggaac agcgccacgg aggtggagta gaaccggtat
    19921 tgatcgttat cccggtagtt gacgctgaat tcaccttccg gtgcgatgcg cgcatttggc
    19981 atctgcaaca atcctgtgcc gccaaagtct gattgcgacg aacccagcgg atccgggtag
    20041 gttaatactt cagcatgaca ggcactggag acggcaatgg ccaggcagct taaaagataa
    20101 gaattcttct tcatcagtca ggaacacgtt gcgttaaaac agacacaatc tggtcattta
    20161 aatcagcata tttttcgggc agtacatgcg cagaaaagcc caaccaaagc tggctgccag
    20221 gcggtggttc cacatgtctt ttattccaca gggcaacagg ggcgacgaca gtctcaccct
    20281 caggcgtaat caccataacg ttattcttgt ccgcgcctgc aaggcggggg tgatcttgca
    20341 gataatcggt aacgctacgg cctgcctgcc agggcaattg cccagcccca gaaaccgcac
    20401 ccagcagagt aatagtcacc ggacgctgta cggtatagag cgtatagtcc ccgaccaacg
    20461 ggggattgct gttctcatcc actcgaacaa aatcagggtc cagtttgacc ggtaagcgac
    20521 cagtgatatt gagattgagt aactgctggc gcacggattt aatcgttgcc gccacatcgt
    20581 cgtcagcttc tgcttcccag gaagcaagct gtgccatgac atgttggtag tctttaagcg
    20641 ctttagcttt cgccgcagag tcggtcagca aggcaccagg ccaccagagg cgatcgcgca
    20701 gttgcggttg tgtcaccaac tggacaacat tttccacggg gccaacggaa agtgtctgtt
    20761 gttcgccagg cagataaata gtcaccgttc cttgcgcaaa ggcatggggt gtcattacgt
    20821 aaagtacgct ggcaatgaaa tacgactgta atttattcat ggtgcgggtt taagaaacgt
    20881 catttctacg ggaataaccc ctgcgcctaa catttgccga ctttgacgaa cctggccagt
    20941 agccgaatcg acccagaatg tgttttgcca ctgtttttct ggccgcgtgg aagtcacctc
    21001 ttcctgccag acattacaca gcacattgcg acccgcaata ttcagcgtct ctaatccagc
    21061 aaaggaaaaa gtagaactca gggtcgcaga gcgaaaatgg ttgtcttcac tccagagaat
    21121 atcgcgcgtc cagcggctgc catcttttat tgccagcgcg ttacgcagag ggtcctgccc
    21181 ggaatttgtc acttccagca gattattatt aagtttgacg gtttttaaaa gacgtccatt
    21241 gtgggtaacc agcatggcgt tatcctggga taaccatttg ctttgttctt gttctatata
    21301 tcccagtacc acaaagattc gttgcccttc attaaggcgt aaatacatcg tggaataagg
    21361 caacgcctgg atctgctgat ccgctacggt gatatcctga ttatcgaaaa ggctggcgcg
    21421 aaatgtatcg accatacttt gctggctgtg cgtacatccc gcaagaaata gtgcgaaaat
    21481 cgataaaata agagggcgca cctttaatcc ttgaaaggtt aattaaaaaa atgcaaatct
    21541 ctaaagcccg gtacgaatat cgcaaccggg tatcatttaa ctttaatttc gacgcgttaa
    21601 agcagtatta gcgtgtagtg gacgtggtgg ttgaggtgtt atgagaaccg ccatcgccac
    21661 tgtcatttgc tgcaacaact gccaacgtcg ccacaccaac agccgttgcc acaccgacaa
    21721 ctgtcgccgt gccgacagcg ccagcagcca cgccaccagt ggtagtagca gtcgtggttg
    21781 cgccggtagt ggtcgttgtg gtgccagtag tacccgtcgt tgcctcggtc ccgtttgttg
    21841 cttcaggggc agcaaacgca gctggcgtcg tcaacatgaa ggccataagg attgcagaaa
    21901 gcttgtgttt cataactttt cctttattca tcgcatggac aatacgtacg cttgagatca
    21961 ggattgtcta ctttgagtat aacatccttg tgaaatctcc ctttcctcat accgcttgca
    22021 ctatatactg cgggtaggaa aggcgcgcag agggaaatat aagattgtta actatccccc
    22081 tctggaaata cgacttgtga gcataagata aatcttagca aaatacaaat taaagataag
    22141 gaaattgtta cgaaagctat taattattgt tggtaatata gtttcaagtg gtactataac
    22201 tggctgttaa atggcattta taactattag gtgcagagat attcgcttaa atggattagt
    22261 ttctttgtgg aaaagtaact gatgttataa tgtatgatgt tgactattgc actattccaa
    22321 taaaaccagt cagctttaaa caagcagcgt catattaaga gagataaaca tttgccgctg
    22381 ttggtcctcg caggccattt acgcggcaaa attccacacg taatcctggt ataagcactt
    22441 ctgcgtcgcg gggagtgaat gcggaaatat ggacctgaac ttctttacga ccgtcggagg
    22501 ggataatgaa tcctttgccg cttttgcgat caaaggtttt gacaattcct gtcattttac
    22561 gggacaaaca aattccttac tgaaaatact gcgctgcact atacggggtt aataaaataa
    22621 agccagcgat atttaagacc gccggacggc taaaataaaa tttgcttaat ctcaattatc
    22681 atgcgttaat agctgcgtcg gtttgaaaga cagacagcat acaaagtagt ttactaaagc
    22741 agttctcatt atcaggcatt atccccttct tttgagtctc tctcctgaac actaagtagt
    22801 ttctgtatta aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa atatgtctaa
    22861 taaaatgact ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc
    22921 tgatgatggc agcaaagacg ttttcgtcca tttcaccgcc atccagagca atgaattccg
    22981 cacgctgaac gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggccccgc
    23041 ggcagcgaac gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct
    23101 gtccatgttg tcatggttca cagtaccgca catcggcatt cgatgtgacg gagcgaaacc
    23161 ctttgggcgc taagtgtatt ttttgtaaat cgacgatgat cacctttgat aacgtcgcgc
    23221 tgcaaatacg cactgaccat gtgccgctgg atttcacaaa ataatatcag gctccctcgt
    23281 ggagcctttt ttatatctgc cttatttttc ttcaacgctg tatgtatagt aagcgataac
    23341 ctgttgatta ttgaatcttt cggggagatg gcttataaca tttcttacct gaccagggta
    23401 ccgggaacca acaccttact ggcgtgttgc tgtctttcaa gaccagaaga ggttaacagt
    23461 gaatattgaa gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa
    23521 aatcgccgag ctgaacaaga atacagggaa agaagtctct gaaattcgct tcaccgcacg
    23581 agaaaaaatg accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga
    23641 acatcacccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
    23701 ttgtggaatt aagcgtcggt acaattcatc cggtaccggg ctttgccata ctcccgcata
    23761 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
    23821 agacaacgca aaggcttttt tatttgccgc aggtttttgc agtgaaaagg tcaatgttga
    23881 ggataccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
    23941 aattaattta tcgaccggga tccgtgatgg gcaatttttt gcgcatttgc cacagtcgat
    24001 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
    24061 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
    24121 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
    24181 aacgtccatc accacgctgt agggcgacaa cataaaatag tgaatcgcct gagcgggcat
    24241 caataacgcg atatagagaa aaaaactcaa cagcaaatac ttcacgccgc gcagaggaat
    24301 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
    24361 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
    24421 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
    24481 acctgttaca aggctatatt tcagattcat cagcccggca atcggtagcc agccttcgat
    24541 acctcccggt ctggcgacaa atgtcgtgct acttgccgtt tcgtaatagc gcacccaata
    24601 ccagaacgtg atggcaatat aaatattcgt tgccaacagt aataattgcg tcgctttacg
    24661 ccaggtcgtg gcattacgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
    24721 ccgctgccag cgggttcttt tattctctgc catgattttg ccagtccgtt aagttgcata
    24781 ccaaatgcca ctattctagt tgttcttaac tggctgatat tgattcaaat cgcgttcagg
    24841 tctttcttat tcaaccatgc ttccagatcg gcaacactgc gtgtaatttc ttcgtgtgga
    24901 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctggctggc gatacgcatt
    24961 cccaaactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctatcc
    25021 tgactggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tgtaaataat
    25081 accagccatt cgtggatctt ctccgtcccc attaactgag catcttcatt gagttgcgat
    25141 acatccagcg attgatcgtt attgacttgc agttggagat agtgcgccag taactgaccg
    25201 agcacttcac gcggcaccgg tttagggata atcccgcgga ataatgaact ggtacgctgg
    25261 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
    25321 tgtgccagtt gtcgggcaag cgtaatgccg tcgacatccg gcagatcaaa atccaccagt
    25381 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaaca
    25441 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
    25501 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt gactgttttt
    25561 ggcacgggtg ccgtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
    25621 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
    25681 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
    25741 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
    25801 ccatcagtgc gactacgcag gataatgtac ccttcgtcag taaaacgcag ggcgttgctc
    25861 aacaggttgg ttataacctg acgaatacgt cgtggatcgc ccattaacgc gcacggcata
    25921 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
    25981 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac cgaaacattc
    26041 ttgccacctg cttcgatagc ggaataatcg agaatatcgt tgaggatggt cagcaacgat
    26101 tcaccagagt cagtaattgc ccgcaaatca tcacgctggg cgttaagtgc ggggttatct
    26161 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
    26221 gccgccagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
    26281 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
    26341 tcgcggtggc gattcagtgc atgaacattg ctgcgaaacg catccatcag ccgcccgatg
    26401 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
    26461 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg ataaaccacg
    26521 cgccaaagga tcagaatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
    26581 tgcccgcgtg cactggcttt ttccagatgc gccagtccgt gctgattacg cagctcaatg
    26641 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat gttattttgt
    26701 gcgagagttt gtaggtgatt gctgatttca ctgtcctgct gatacagcgc cagcaaatcg
    26761 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaacacc cggatcttca
    26821 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
    26881 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
    26941 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
    27001 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
    27061 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgcgccag gcgtgcgatc
    27121 tcatcggcgg cagcgactat ctgctgactg agttgccgtt gttgctggcg tagttgcaga
    27181 cgccgcccca ccagttcccc ttgctgacgt aatgaacggg agatctcctg ctcctgttgt
    27241 tcaatagcgg tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc attaatcttc
    27301 aggctttgtg cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt atcggcactg
    27361 gtcaggttct gcgcggcgaa aagttcccag gcgctggctt cgctcaactg gcgcgccata
    27421 ttcatggtag gaatcaatgc ctgagtattg tctttttcca cctggctgat aaagcgcagg
    27481 ttgtaccatc ccaccagggt actggtcagg gttaacagcg ccatcagggc aaagcccatc
    27541 cagagtcttc gggtcagggt taaattcacg gtcggtgcac tttaggtgaa aaaggttgag
    27601 tcgcaaagcg gaatgcatct agcataaagc cttattattg atgaggctat catgcgcgta
    27661 ctgctatttt tacttctttc ccttttcatg ttgccggcat tttcggctga taacctgttg
    27721 cgctggcatg atgcgcagca tttcacggtg caagcctcta cgccgcttaa agccaaacgc
    27781 gcatggaaac tgtgcgcgct ttatcccagc ctgaaagatt catattggtt atcgttgaac
    27841 tatggtatgc aggaggctgc tcgccgctac ggtgtggatt taaaagtgct ggaggcaggc
    27901 ggctacagcc agttggctac ccagcaagca caaatcgacc agtgtaaaca gtggggcgca
    27961 gaggccattt tgctcggtag tagcacgacc tcatttcccg acctgcaaaa gcaggtagca
    28021 agtctgccgg tgatcgaact ggtaaatgct attgatgctc cccaggtgaa aagccgcgtt
    28081 ggtgtgccct ggtttcagat gggctatcaa ccggggcgat atctggtgca atgggcgcac
    28141 ggtaaaccac tgaatgtgct gttgatgccc ggacccgata acgccggggg cagtaaggag
    28201 atggtcgagg gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt tgatattgcg
    28261 cttggtgata acgatattga aatccagcgt aacctgttgc aggagatgct ggaacgccac
    28321 ccagaaatcg acgtcgttgc cggaacggcc attgcggcag aggcggcaat gggtgaaggg
    28381 cgtaacctga aaacgccgct taccgtggtg tcgttttatc tttcacatca ggtgtatcgc
    28441 gggctgaagc ggggaagagt gattatggct gccagcgatc aaatggtctg gcagggggaa
    28501 ctggcagttg agcaggccat caggcaatta caggggcaat cggtgtctga taatgtcagc
    28561 ccaccgattt tagttctgac gccgaaaaat gccgaccgag aacatatccg ccgctcgctg
    28621 tcaccagggg gatttcgtcc ggtctatttt tatcagcaca catcagcggc taagaaataa
    28681 ccttcaccat gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa acgacgaatt
    28741 aacacatcga cggtgcgcag gtcagggttt tccacccgac gcgcagaaag catacgtagc
    28801 agacgttcac ggctgagaat ttcgcccgga ttcgtcacaa atgccaccaa catttcatac
    28861 tctgcgcggg tcagtttaat cggctcgcca tcccgctcca gcgtatggcg cgacacattc
    28921 aggcaataac cggcaaagcg atagcagttg tcctgagtgt gcggttgagc ttgtcgcgcg
    28981 aggtcgattc gccagagcag atttttcacc cgtactacca gttcgcgcag ttccagcggt
    29041 ttggtgacgt aatcgtctgc gcccatttcc agcccaacaa tacggtcaat ccgatcgctg
    29101 cgtccggtaa ccagaataat ccccaccgtt gagcgttctc gcagggcgcg ggttaacatc
    29161 aggccatttt catcgggtaa gttgatatcc agcagaatta aatctaccgg ctgattctgc
    29221 ataatttccc gcagcccggc accgctcgct gtaacggaaa cggtataccc ctcctgagtg
    29281 aagtaagatt gtaatcgcgc ctgggtaacc ggctcatctt caacaataac aatgtgatgt
    29341 ggcatcagag ggttttactc attctgttca tatctgttca tattctgccg taagccgttc
    29401 atcctgacca gtgccgctgt tcatatttgc tcattaagat cgcttcacta aaccataatt
    29461 ttacaggggc tattatgcgg aaactctgga acgcgctacg ccgacccagt gctcgttggt
    29521 cggtactggc gctggtcgca attgggattg tgattggcat tgcgctgatt gtattgccac
    29581 acgttgggat caaagtcacc agcacaaccg aattttgtgt cagttgccac agtatgcaac
    29641 cggtgtatga agaatataaa cagtcggtgc atttccagaa cgcctccggc gtgcgagctg
    29701 aatgccatga ctgtcatatc ccgccggata ttccaggcat ggtgaagcgc aaactggaag
    29761 cgagcaatga tatctaccag acctttattg ctcactccat tgatacacct gaaaaattcg
    29821 aagccaaacg cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa aacaactcgg
    29881 caacctgccg ctcctgccat aactacgatg cgatggatca tgcgaagcag catcctgaag
    29941 cggcacgtca gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac tgtcataaag
    30001 gtattgccca ccagttaccg gatatgagta gcggcttccg taagcagttc gatgagctgc
    30061 gcgccagtgc taatgacagt ggtgacacgc tgtactctat tgatattaag ccaatttatg
    30121 cggcgaaagg cgataaagaa gcctctggtt ctctgctgcc tgcttcggaa gtgaaagtcc
    30181 ttaaacgtga cggcgactgg ctgcaaattg aaattaccgg ctggacggaa agcgccggac
    30241 gtcagcgtgt actcacccaa ttcccaggta aacgcatctt tgttgcctcg attcgtggtg
    30301 atgtgcagca gcaggtaaaa acgctggaga aaaccaccgt tgccgacacc aataccgagt
    30361 ggagcaagtt gcaggccact gcgtggatga agaaaggcga catggtgaac gatatcaaac
    30421 cgatctgggc ttatgcggat tcgttgtaca acggcacctg taaccagtgc cacggcgcac
    30481 cggaaatcgc ccactttgac gctaacggtt ggatcggcac gctcaacggc atgattggct
    30541 ttaccagtct cgataaacgt gaagaacgca ccttgttgaa atatctgcaa atgaatgcgt
    30601 ctgacaccgc aggtaaggct cacggcgata agaaggaaga aaaataatga acaataacga
    30661 tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg
    30721 gatgctgggg ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag cggcgactga
    30781 cgctgtcatc tcgaaagagg gcattcttac cgggtcgcac tggggggcta tccgcgcgac
    30841 ggtgaaggat ggtcgctttg tggcggcaaa accgttcgaa ctggataaat atccgtcgaa
    30901 aatgattgcc ggattgccgg atcacgtaca caacgcggcg cgtattcgtt atccgatggt
    30961 acgcgtggac tggctgcgta agcgccatct cagcgatacc tcccagcgcg gtgataaccg
    31021 ttttgtgcgc gtgagctggg atgaagccct cgacatgttc tatgaagaac tggaacgcgt
    31081 gcagaaaact cacgggccga gtgccttgct gaccgccagt ggttggcaat cgacggggat
    31141 gttccataac gcttcgggga tgctggcgaa agctattgcc ttgcatggta atagcgttgg
    31201 tacgggcgga gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg tggttggctc
    31261 aatggaagtg tatgaacagc aaacctcctg gccgctggta ttgcagaaca gcaaaaccat
    31321 tgtgctgtgg ggctctgatt tgctgaaaaa ccagcaagcg aactggtggt gcccggatca
    31381 cgatgtttat gaatattacg cgcagctaaa agcgaaagtc gccgccggtg aaattgaggt
    31441 catcagcatc gatccggttg tcacatccac ccatgagtat ctggggcgcg agcatgtgaa
    31501 gcacattgcg gttaacccgc aaactgatgt gccgctgcaa ctggcgctgg cgtatacgct
    31561 gtacagtgaa aacctgtaca acaaaaactt cctcgctaac tactgtgtgg gttttgagca
    31621 gttcctgccg tacctgctgg gtgagaaaga cggtcagccg aaagatgccg catgggctga
    31681 aaaactgacc ggcattgatg ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa
    31741 cagaacgcaa attattgctg gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc
    31801 gtggatgatt gtggttctgg cggcgatgct ggggcaaatt ggcctgccag gtggtggctt
    31861 tggttttggc tggcactata acggcgcagg cacgccgggg cgtaaaggcg ttattctgag
    31921 tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgatt acaaaggcta
    31981 cagcagcacc attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat
    32041 caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac
    32101 taacccattc catcgccatc agcagatcaa ccgtattatt gaaggctggc gcaagctgga
    32161 aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct
    32221 gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg
    32281 tggcattatc gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga
    32341 tattttccgc gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga
    32401 cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg
    32461 cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca
    32521 tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg
    32581 cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga
    32641 ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc
    32701 gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagtt
    32761 atgtgagtcg gaaactctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat
    32821 taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa
    32881 cgctcgcggt caggtgttgg caggggcagt agtttctgac cgctatgcac ccggcgtggc
    32941 acgaattcac gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg
    33001 caaatacggt aaccccaacg tgctgaccat cgacatcggt acttcgcagc tggcgcaggc
    33061 gaccagtgcg cacactacgc tggtggaaat tgagaagtac aatggagcag tggagcaggt
    33121 gacggcgttt aacggccctg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca
    33181 ggtgaaatca tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg
    33241 cagttgttct cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg
    33301 gctgaatggt tttcgttgct gaaaagcgaa ccgccgctgg ctgcggcggt gaatgagctg
    33361 gaaaactgta ttgccacgct gacagtacgt gacgatgccc gtctggaact ggccgctgac
    33421 ttttgcggcc tgtttctgat gaccgacaaa caagcggcgc tgccgtatgc atcggcctac
    33481 aaacaggacg agcaagagat taaacgcttg ttagttgagg cagggatgga aaccagcggc
    33541 aatttcaacg aaccggcaga tcatctggcg atctatctcg aattgctcag ccatctgcat
    33601 ttttcgctgg gagaggggac cgttcctgcg cgaagaatcg acagtttgcg gcaaaaaaca
    33661 ctgacggcgc tgtggcaatg gttaccagag tttgttgtgc gttgtcgtca gtatgacagc
    33721 tttggttttt acgcggcact aagccagtta ttgctggtgt tagtggagag cgaccaccaa
    33781 aacagataac gtcgtttgtg cgcctgaaaa gacgcgttta gcgtcgcatc aggcattatg
    33841 gggcagttgc cggatgcgtc atcacatccg gcaaacacac atcacggatg agctacaaac
    33901 cgggaaagtc gctggcgcag caggcggttt tcctgcttca ggtgcgcaat atcatccatt
    33961 aacgtcagcg ccaccgcgat cccaggccag tccagagcca gttcatgacg caggcgtact
    34021 gcgcgttgca ccacgatggc ggcatggtcg tcaaataccc aggttgtttc ctggatctca
    34081 cgcggttcaa ccacccccaa accgacaatt tcattcaact cctcttcaga gatgccggta
    34141 tgcaggcaaa attcggtaat agtaaaagtc accgtaacat tagccattat gctttccccc
    34201 aatctttacg tggatcaaaa gacgactggg cgtctgccag ttgctgccac agcgcggcag
    34261 tgttttcatc cggtttcggc ggcatcacga ttttcagtac cgcatacaga tcgccggtct
    34321 gttttttgct caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc tggctgcctg
    34381 gcgggatagt cagcaaaatg ctttctttca gtgttggaac ggtgacttta gcacccagcg
    34441 ccgcttccca cgggctaacc ggcaccacaa tttccagatc ctggccgaca atatcgaaca
    34501 gcggatgtgg cgcaatatga atcaccagcc acaaatcgcc atttggacca ccgttttcgc
    34561 ccggcgtccc ctggcctttc agacggatgc gttgaccatt gccgacgccc gccgggatct
    34621 tcacattcag cgttttcggg atttcctgtt cgatcatgcc aaaggcgtta taaaccggca
    34681 ggttatagct gatggtacgc ttatgctcag taagcgtttc ttcgaggaat accgccactt
    34741 cgatttcaat atcgtggccg cgtgcggcgg ggcgttgacg gctctggcgg gcatgctgac
    34801 cgaaaattga cgagaagata tcgtcaaaat cttcggcatt aaaactctga ccgtcgccat
    34861 gatggaactg acggttaaat tgcggatcgt tgcgatgttg ccacatctga tcatactcag
    34921 cgcgacgttg ttcatcactt aacacttccc aggcttcagc gacctctttg aagcgggctt
    34981 cggcatccgg ttctttgctg acatcaggat ggtatttgcg ggccagtcga cgataggcgg
    35041 tcttgattgt cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa taatccttta
    35101 attccatagc gttatctcgc gtaaatcaac acaaattgaa ggaacccctg taaggtaact
    35161 cctataagtg tagggtaatc ctcaaaattt catatgccaa cacagaatat gttattgaaa
    35221 tcatcgcgga gaggaggtcg cctcaaggtg gggtgctgaa catattttaa acaggtgaaa
    35281 aagggtgagc gatttttgat agtggaacca ggcactttaa gtttaactgg ggcgtcatta
    35341 tttattaaat t
//
LOCUS       DAPPNJ010000023        34564 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000023 DAPPNJ010000000
VERSION     DAPPNJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.023"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            20..2425
                     /locus_tag="RPM73_000880"
     CDS             20..2425
                     /locus_tag="RPM73_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDX6519270.1"
                     /translation="MGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQAREKV
                     VWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHP
                     DRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGN
                     MTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLV
                     VMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTD
                     AALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAK
                     TPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTG
                     NVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRG
                     KDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTSSAKYADI
                     LLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQ
                     KFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPE
                     ANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRTFPLQL
                     FGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPA
                     KVTPRILPGVSAMGQGVWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEI
                     EKI"
     gene            2486..4909
                     /locus_tag="RPM73_000881"
     CDS             2486..4909
                     /locus_tag="RPM73_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDX6519271.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4920..5537
                     /gene="dmsB"
                     /locus_tag="RPM73_000882"
     CDS             4920..5537
                     /gene="dmsB"
                     /locus_tag="RPM73_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDX6519272.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGTPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5539..6393
                     /locus_tag="RPM73_000883"
     CDS             5539..6393
                     /locus_tag="RPM73_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDX6519273.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6436..7050
                     /gene="dmsD"
                     /locus_tag="RPM73_000884"
     CDS             6436..7050
                     /gene="dmsD"
                     /locus_tag="RPM73_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDX6519274.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7209..8501
                     /gene="clcB"
                     /locus_tag="RPM73_000885"
     CDS             7209..8501
                     /gene="clcB"
                     /locus_tag="RPM73_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDX6519275.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     VIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8454..9149)
                     /gene="bioD"
                     /locus_tag="RPM73_000886"
     CDS             complement(8454..9149)
                     /gene="bioD"
                     /locus_tag="RPM73_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDX6519276.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9274..10494)
                     /gene="mlc"
                     /locus_tag="RPM73_000887"
                     /gene_synonym="dgsA"
     CDS             complement(9274..10494)
                     /gene="mlc"
                     /locus_tag="RPM73_000887"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDX6519277.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10629..11522)
                     /locus_tag="RPM73_000888"
     CDS             complement(10629..11522)
                     /locus_tag="RPM73_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6519278.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11629..12882
                     /locus_tag="RPM73_000889"
     CDS             11629..12882
                     /locus_tag="RPM73_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519279.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFSLASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            13279..13614
                     /locus_tag="RPM73_000890"
     CDS             13279..13614
                     /locus_tag="RPM73_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDX6519280.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            13707..13790
                     /locus_tag="RPM73_000891"
     CDS             13707..13790
                     /locus_tag="RPM73_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519281.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13890..14711
                     /locus_tag="RPM73_000892"
     CDS             13890..14711
                     /locus_tag="RPM73_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDX6519282.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14750..15079)
                     /gene="mdtI"
                     /locus_tag="RPM73_000893"
     CDS             complement(14750..15079)
                     /gene="mdtI"
                     /locus_tag="RPM73_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDX6519283.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15066..15431)
                     /gene="mdtJ"
                     /locus_tag="RPM73_000894"
     CDS             complement(15066..15431)
                     /gene="mdtJ"
                     /locus_tag="RPM73_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDX6519284.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15843..16877
                     /gene="tqsA"
                     /locus_tag="RPM73_000895"
     CDS             15843..16877
                     /gene="tqsA"
                     /locus_tag="RPM73_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDX6519285.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNFIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16902..18290)
                     /gene="pntB"
                     /locus_tag="RPM73_000896"
     CDS             complement(16902..18290)
                     /gene="pntB"
                     /locus_tag="RPM73_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDX6519286.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18301..19833)
                     /gene="pntA"
                     /locus_tag="RPM73_000897"
     CDS             complement(18301..19833)
                     /gene="pntA"
                     /locus_tag="RPM73_000897"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDX6519287.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20357..21301
                     /locus_tag="RPM73_000898"
     CDS             20357..21301
                     /locus_tag="RPM73_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6519288.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21487..22869
                     /locus_tag="RPM73_000899"
     CDS             21487..22869
                     /locus_tag="RPM73_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6519289.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPIHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22906..23628
                     /gene="folM"
                     /locus_tag="RPM73_000900"
     CDS             22906..23628
                     /gene="folM"
                     /locus_tag="RPM73_000900"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDX6519290.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23625..23960)
                     /locus_tag="RPM73_000901"
     CDS             complement(23625..23960)
                     /locus_tag="RPM73_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDX6519291.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24089..24808
                     /gene="rstA"
                     /locus_tag="RPM73_000902"
     CDS             24089..24808
                     /gene="rstA"
                     /locus_tag="RPM73_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDX6519292.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24812..26113
                     /gene="rstB"
                     /locus_tag="RPM73_000903"
     CDS             24812..26113
                     /gene="rstB"
                     /locus_tag="RPM73_000903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDX6519293.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26189..27118
                     /gene="tus"
                     /locus_tag="RPM73_000904"
     CDS             26189..27118
                     /gene="tus"
                     /locus_tag="RPM73_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDX6519294.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27115..28518)
                     /gene="fumC"
                     /locus_tag="RPM73_000905"
     CDS             complement(27115..28518)
                     /gene="fumC"
                     /locus_tag="RPM73_000905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDX6519295.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGR"
     gene            complement(28660..30306)
                     /gene="fumB"
                     /locus_tag="RPM73_000906"
     CDS             complement(28660..30306)
                     /gene="fumB"
                     /locus_tag="RPM73_000906"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDX6519296.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30505..31680
                     /gene="manA"
                     /locus_tag="RPM73_000907"
     CDS             30505..31680
                     /gene="manA"
                     /locus_tag="RPM73_000907"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDX6519297.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            31781..33289
                     /locus_tag="RPM73_000908"
     CDS             31781..33289
                     /locus_tag="RPM73_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDX6519298.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(33334..>34564)
                     /gene="uidC"
                     /locus_tag="RPM73_000909"
     CDS             complement(33334..>34564)
                     /gene="uidC"
                     /locus_tag="RPM73_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDX6519299.1"
                     /translation="TAASGLTSAYAAQLADDEAGLRIRLKNELRRADKPSAGAGRDIY
                     AWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFALGAVKI
                     KPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTPNIDLWG
                     MWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLGASVQKD
                     VSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQLTWNAPW
                     GSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQFTLIFA
                     PIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGREKRDGSTLGDR
                     LNYWDVKMSIQYDFMLK"
BASE COUNT         8499 a         8932 c         8510 g         8623 t
ORIGIN      
        1 gtccaaaaat gaacgaatgg tgggcatcag ccgcagaaca ctcgttaaat ctaccgcgat
       61 aggttctctg gcgctggctg caggcggttt ttctttgccg tttaccctgc gcagtgcagc
      121 agcagcggta caacaggccc gcgaaaaagt ggtctggggt gcctgttccg tcaactgtgg
      181 tagccgctgt gcacttcgtc tacatgttaa agataatgaa gtgacctggg tggaaactga
      241 caataccggc agcgatgagt acggcaacca tcaggtacgc gcctgtttgc gcggtcgctc
      301 catccgccgg cgtattaatc atcccgatcg cttgaattac ccaatgaaac gcgtgggtaa
      361 acgcggtgaa ggcaaattcg aacggattag ctgggatgaa gccctggata ctatcgccag
      421 tagcctgaaa aaaaccgtcg aacaatatgg caatgaggct gtatatattc agtactcttc
      481 ggggattgtt ggcggcaata tgacccgctc ttcgccatca gcctcggcgg tcaaacgcct
      541 gatgaactgc tacggcggtt cactcaatca gtatggctcc tacagcaccg cgcaaatttc
      601 ctgtgccatg ccctacacct acggcagtaa tgatggcaac agcaccacgg atattgaaaa
      661 cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa acccgcatga gcggcggtgg
      721 catcacttat cttcttgaaa aagcgcgcga gaaatcgaac gccaaaatga ttgttatcga
      781 tccgcgatat accgatacgg ctgctggtcg tgaagatgaa tggctcccta ttcgcccggg
      841 caccgatgcc gcgctggttg cgggtattgc ctgggtattg attaacgaaa atctcgttga
      901 tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa aaaaccttac ccgcagatgc
      961 acccaaaaat ggtcactata aagcctatat tcttggtgaa ggtgatgata acacagcgaa
     1021 aacgccgcag tgggcttcgc aaattaccgg tatcccggta gaccgtatca tcaaactggc
     1081 gcgtgaaatt ggcacagcaa aacccgctta tatctgccag ggctgggggc cgcaacgcca
     1141 ggcaaacggc gaactgactg cacgcgctat tgctatgtta cctattttga cgggcaatgt
     1201 cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc tataccatta ccatagaacg
     1261 cctgccggtg ctggataatc cggtcaaaac gtcaatctcc tgcttcagct ggacagatgc
     1321 tatcgatcat ggtccgcaaa tgacggcaat ccgcgacggc gtccgcggca aagataaact
     1381 ggatgtgccc attaagttca tctggaacta cgcgggaaat accctcgtta atcagcattc
     1441 tgacatcaac aaaacgcatg aaattctgca ggacgaatcg aaatgcgaaa tgattgtgat
     1501 catcgaaaac tttatgacct catcggcaaa atatgccgac attctgctgc cagacctgat
     1561 gaccgttgag caggaagata ttattcctaa cgactacgcc ggtaacatgg gatatctcat
     1621 tttcctccag cctgtcacca gcgaaaaatt cgaacgcaaa ccgatttact ggatcctgag
     1681 tgaagtcgcg aaacgtcttg gacctgacgt ctatcaaaag ttcacagaag gtcgcacgca
     1741 ggaacaatgg ttacaacatc tgtacgccaa aatgcttgcc aaagatccgg cgttaccgtc
     1801 ttacgacgaa ctgaaaaaaa tgggtatcta taagcgtaaa gatcccaatg gccattttgt
     1861 cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca cttaaaacgc cttccggtaa
     1921 gattgaaatt tattccagca ggctggcgga aattgcccgt acctgggaac tggaaaaaga
     1981 tgaagtgata agcccattgc cagtttatgc ctcaaccttt gaaggctgga actcccctga
     2041 acgtagaacc ttcccactgc aactgtttgg tttccattac aaatcccgta ctcactcgac
     2101 ctacggcaat attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat
     2161 agatgcgcag aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg
     2221 cgaagttcgg ctaccagcca aagtaacacc acgtattctc cctggagtta gcgctatggg
     2281 ccagggagtc tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt
     2341 gaatacgcta accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa
     2401 tctggtcgag atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg
     2461 tttgaaaagc aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc
     2521 tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag
     2581 cagtgctttc actctgccat tttcccaaat ggtccgggcg gcagaggctc cggtagaaga
     2641 gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca
     2701 tgtgaaagat gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg
     2761 taaccatcag gttcgagcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc
     2821 tgataggttg aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggta aatttgaacg
     2881 gataagttgg gacgaagccc tggataccat cagtgataat cttcggcgga tcctgaaaga
     2941 ttacggcaat gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac
     3001 caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg
     3061 cagctacagt accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg
     3121 caacagcccg gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc
     3181 agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc
     3241 aaacgcacgc atgatcgtca tcgatccacg ttataacgac actgctgccg ggcgtgaaga
     3301 tgaatggctg cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt
     3361 actgattact gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga
     3421 tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg
     3481 cgaagggcct gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc
     3541 ggcagaaaaa attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg
     3601 tcagggttgg gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat
     3661 gctttccgtt ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg
     3721 tagctgggat ctgggggtag aatggttccc gatgcttgag aaccctgtta aaacgcagat
     3781 ttccgtcttt acatggacag atgccatcga ccatggtaag gaaatgaccg cgacccgtga
     3841 tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag
     3901 taacacattg atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga
     3961 cagcaagtgc gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg
     4021 cgatatcctg ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc
     4081 tgcagggaat atgggctatg tgatcctcgc ccaacccgca acctctgcaa aatttgaacg
     4141 caaacccatc tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca
     4201 aacctttact gaaggtcgca gtcagcatga atggatcaaa tatctccatg cgaaaacgaa
     4261 ggaacgtaac cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa
     4321 aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cccaggcaaa
     4381 cccgttgaaa acaccttcgg ggaaaatcga aatttattct gaacgactgg cgaagattgc
     4441 ggatacctgg gaattgaaaa aagatgaaat tattcatccc cttccagctt atacgccagg
     4501 ttttgatggc tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca
     4561 ctacaaggcg cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc
     4621 acaagaggtg tggatcaacc ccattgatgc tcaggctcgc ggtatccgtc atggcgatac
     4681 cgtgcgggta tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat
     4741 tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga
     4801 ccgggtcgat cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc
     4861 aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaaa gagtagccca
     4921 tgaccacaca atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg
     4981 aactggcgtg caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg
     5041 aatatgctgg cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt
     5101 attatctctc catctcctgt aaccattgcg atgacccagc atgtacaaaa gtctgcccca
     5161 gcggcgcaat gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg
     5221 gctgccgcta ctgccacatg gcttgtccgt acggcacacc acagtacaat gctgaaaaag
     5281 ggcacatgac gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat
     5341 gcgtcgaatc ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga
     5401 agcacggcac tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata
     5461 tcgttatcaa acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta
     5521 atccggagga agtgtaaaat gggaaatgga tggcatgagt ggccactggt tatctttacc
     5581 gtattgggtc aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc
     5641 aaaaatgacg cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg
     5701 ggcatcgggt ttattgcctc tgtcatgcac ctgggatcgc cactgcgagc gtttaactcg
     5761 ctgaatcgaa tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt
     5821 gccgttggcg gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg
     5881 aaactctggt tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc
     5941 gtgtatcaaa tcgacaccgt gccaacctgg cataacggtt acactacgct ggctttcttc
     6001 ctgacggtat tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agctcgcgtt
     6061 acatttaata ccacaccatt tgccatcatt agcgttttgg cgctgatcgc ttgtgccggg
     6121 gtgattgtgc tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc
     6181 gcactggtac cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt
     6241 cttggatgct ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt
     6301 ctggggctaa tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg
     6361 cacatgacag taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat
     6421 ttcaggatgt taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc
     6481 gtgttgggcg cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg
     6541 gtactcacca gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg
     6601 ctggtgacgg cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg
     6661 tttgtcggcc cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa
     6721 tctgtgctgt ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt
     6781 cagtttgaaa tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct
     6841 gcgtggttgg cggaaaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt
     6901 tttccgtggt caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac
     6961 cgtgcactgg gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt
     7021 cctgtcgcgg ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga
     7081 ttacgcgcaa tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg
     7141 cactgctttt gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat
     7201 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
     7261 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga
     7321 tgctgctact ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga
     7381 caaacctttc cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt
     7441 tgttgctgat gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca
     7501 tggaagcgtt gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg
     7561 cctcgctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
     7621 tagctgccct tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat
     7681 tatggatcgc ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg
     7741 gcagtttatt tatcgccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
     7801 tgattatttc cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt
     7861 tactctacaa cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
     7921 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgcc
     7981 atcgtggatt tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcggggtga
     8041 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
     8101 aatccttttt aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac
     8161 tgtgtgccgt gctggcgagt agcggttccg gcgcacccgg tggggtcttt acaccgacgc
     8221 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
     8281 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
     8341 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgactggg gagtatcagc
     8401 tactccccgg tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc
     8461 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
     8521 tctcgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
     8581 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc
     8641 acccagccaa tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg
     8701 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcaactgttc ctgcaccacc
     8761 cattcagaga gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
     8821 acgacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
     8881 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
     8941 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
     9001 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
     9061 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
     9121 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
     9181 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
     9241 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
     9301 agaaccgtta tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga
     9361 aaactgagta ctctcaacgc taatgtgctg actatacgca ggaagggcct gctgacggat
     9421 gctgtctgag atgaccggga agaggatatc tgccgcttta cttaacggtg aaccaatcag
     9481 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
     9541 caccccggta atgatgtctt ttgccaacag atcgccgcgc aatgccgcct gacacaatga
     9601 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg
     9661 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
     9721 ataacagcgt ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct
     9781 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
     9841 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca
     9901 tgcgctgata tcatgctgaa tataaaccgg aacgccggta tgctgctcca gcgcctcgcc
     9961 gagcggcatc tcttttacat cctcatagaa cggcatgcga tgtacaatgc cattttccgt
    10021 atcaataatt cccggcaggg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg
    10081 gcggataaaa aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt
    10141 taacgccagt tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag
    10201 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    10261 cagccccacc gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    10321 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    10381 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat ataccgcgcc
    10441 cgcgttggtc tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata
    10501 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    10561 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    10621 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agctggactt
    10681 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    10741 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg aagcaggcaa aattgaaaca
    10801 cccagaccgg cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg
    10861 acgggcgtca aatggtaacg tcgcatcagt ccgagaatat cgtcatacag ccctgtcccg
    10921 acgtgcggat caaaaaagac aaagggttct ttcgccagtt cagccagcgt tacattcggg
    10981 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    11041 gcgtgttcaa gcgtctccgg taacgctgtg ttacgcagca atcccatatc cagcgttcct
    11101 tcaatgagcg gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg
    11161 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    11221 gaagtaaaac caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg
    11281 gcagcgtcat ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct
    11341 gccgtcagca atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc
    11401 agtgcctgaa tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    11461 ccgaaatgca gctcttccgc aacagcaaca aagtaacgca gatgacgaag ttcaatattc
    11521 atatttaaaa catcttattt gagattatta atatattaga cagaacaatt cgattttcct
    11581 aacctatgta taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact
    11641 acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat
    11701 caatttatta aacgcggtac gccgcaattt atgcgcgtca ccctggcgct gttctctgcc
    11761 ggactggcaa ccttcgcact tctctattgt gtgcaaccta tccttccggt gctttcgcag
    11821 gagtttggct taacgcccgc gaacagtagt atttcactgt ccatttccac ggcgatgttg
    11881 gctattggtt tgctgtttac tggcccccta tccgatgcca ttggtcgcaa accagtgatg
    11941 gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg
    12001 cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt
    12061 ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg
    12121 tatatcagcg gcaactcaat tggcggcatg agcggacgct taattagtgg tgtcttcacg
    12181 gactttttca actggcgaat tgctctggcg gcaatcggtt gtttctcgct ggcctcggcg
    12241 ttgatgttct ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgccct
    12301 aagacgttgt ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc
    12361 gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggctatcgg
    12421 ttgatgctct ccccctggca tgtcagtcag gccgtggttg gcttattatc gctggcttat
    12481 ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta tgggcgcggt
    12541 ccggtgatgt tgttttcgac gggagttatg ctatttggtt tactgatgac cttatttagc
    12601 tcgctgtggc tgatctttgc cggaatgtta ctcttctcag caggattctt cgcagcccac
    12661 tcagttgcca gcagctggat cggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg
    12721 ctgtatctgt tcagttacta tctgggatcg agtattgccg ggacgctggg tggtgttttc
    12781 tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct
    12841 ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag tccggactgc
    12901 ggtaaatacc cgtccggact tattgcctgc tcaaaccaac gttaatagcc atcctaaaat
    12961 agacgaagcg ccagccaatt cccgcagcgc gtctagcgtc atcaggatta taagtaccca
    13021 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    13081 ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc taaatttccc
    13141 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    13201 cacgctgaaa ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg
    13261 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    13321 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
    13381 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac tacacatcat
    13441 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    13501 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    13561 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
    13621 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc ggataacgtt
    13681 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    13741 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
    13801 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    13861 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
    13921 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    13981 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    14041 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
    14101 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
    14161 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
    14221 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
    14281 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcggc ttgattgtgc
    14341 tacgtaatcc cccttctggc attacgccgt tgccgttatt tgagggagat aaagccgcgc
    14401 ttaccgccgc attgaaagcg gcaggtcgta aagtgactca ggcaggatac cctgaagatc
    14461 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    14521 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
    14581 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    14641 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
    14701 cgcaaaaata atgttcaaac gctgcccgac agcgcgggca gcgtcttcat caggcaagtt
    14761 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
    14821 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    14881 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
    14941 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    15001 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    15061 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
    15121 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
    15181 aaactttcgt cgaataacaa aacgctgaac aaggtaataa ataaaatacc gataccttcc
    15241 cacaacgcat aagctacgcc taaggcgatt tttttaacgg cgaaagagag aaatatatac
    15301 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    15361 catttcattg acagcgtacc ggtaatttct gtagcaatag ccagacctaa taaaatccaa
    15421 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    15481 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
    15541 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
    15601 ggtaaatgta gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
    15661 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
    15721 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
    15781 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa ccggggacaa
    15841 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
    15901 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
    15961 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
    16021 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
    16081 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
    16141 ttattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
    16201 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
    16261 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
    16321 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
    16381 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctaa
    16441 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
    16501 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
    16561 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
    16621 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
    16681 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
    16741 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
    16801 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
    16861 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
    16921 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
    16981 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
    17041 gctttccaca cttccagaac aggcatacca gcaataggac tcttcggatc atcctgcgca
    17101 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
    17161 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
    17221 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
    17281 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
    17341 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
    17401 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
    17461 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
    17521 taagaaagga tagcgcccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
    17581 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
    17641 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
    17701 atggcggtca ttatcagcaa tgccagcact tgcaggccga cgctatccgt gcgaacaaat
    17761 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
    17821 agcatcaatg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
    17881 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
    17941 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
    18001 gccagaccca cgaagctatg caggatcgcc accagttctg gcatttcggt catttcaact
    18061 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
    18121 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
    18181 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
    18241 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
    18301 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
    18361 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
    18421 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
    18481 tgtatgcagc gcatgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
    18541 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
    18601 gatgattgcc agcgccatca atgcgtattt acgccacggt gagcaggcac atttttcctc
    18661 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
    18721 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
    18781 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
    18841 gaggttagta ccgtaaagct gtgaggattg cgtcggcaga cggcccggaa gatcggtata
    18901 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
    18961 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
    19021 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
    19081 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttga tgaacgcgtc
    19141 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
    19201 gaattccgcg cccatacttt gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg
    19261 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
    19321 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
    19381 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
    19441 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
    19501 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
    19561 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
    19621 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
    19681 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
    19741 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
    19801 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
    19861 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
    19921 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
    19981 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
    20041 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
    20101 aacgagacat cgccccggca cgaatcacta cttaacatta aattaactta tacaattcag
    20161 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
    20221 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
    20281 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
    20341 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
    20401 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
    20461 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
    20521 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
    20581 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
    20641 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
    20701 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
    20761 agtcaatgat gccatcacca aagcagcaaa agcgaaaggt gcctactctt tctacattgt
    20821 tcgtcaaatc gatgccaacc agggcggcaa ccagcgtatt actgcattca tctataaaaa
    20881 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
    20941 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc caggtgttgc
    21001 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
    21061 cggacgttac actgtcacgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
    21121 agcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
    21181 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
    21241 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
    21301 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
    21361 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
    21421 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
    21481 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
    21541 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
    21601 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
    21661 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
    21721 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
    21781 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
    21841 ttgcgcctgt tcggcgatgg taatacctgg caatcgatag tcggtgcatc ggctttattg
    21901 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
    21961 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
    22021 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtgtgggaa
    22081 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
    22141 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
    22201 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggcgt ggtggcacgt
    22261 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
    22321 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
    22381 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
    22441 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcgctgtg gttgaccaac
    22501 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
    22561 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
    22621 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
    22681 tggttattgt atgcctctgg tccaatacac ttgctgttat ccgttgttct gtatgcgccg
    22741 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
    22801 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
    22861 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
    22921 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
    22981 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
    23041 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
    23101 atggcgtttg ccgatgaagt actaaaaagc acccatggtc tgcgtgctat tttgcataac
    23161 gccagtgcgt ggatggcgga aaaaccaggt acaccactgg ccgacgtact ggcttgcatg
    23221 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    23281 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    23341 agcgacaaac atgttgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    23401 tttgcccgca agctggcacc ggaagtgaaa gtgaattcta ttgcgccatc gctgatcctg
    23461 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    23521 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagctgcttt
    23581 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    23641 cagcatataa tcaacaccca tgcgcttatc ccccaacacg caacagaacc aacaaaagcg
    23701 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    23761 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    23821 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    23881 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    23941 gctttgatta ccagccccat ctttttacct taacacttcc ataacaagtc atcagtagaa
    24001 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    24061 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    24121 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    24181 accgcgcggc gaccaggccg aagaaaccat tttgcgagaa agtccggatt tggtgttact
    24241 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    24301 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    24361 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    24421 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    24481 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    24541 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    24601 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    24661 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    24721 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    24781 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    24841 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggttggtgt acaaatttac
    24901 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcgc tgtatctgat
    24961 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    25021 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    25081 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    25141 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    25201 tctccatcag atgcgattgc tggatatcgc cctgatcgct tttattgcta tttccctcgc
    25261 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    25321 ggcgcaacga tttggcgatg ggcatctcaa tgagcgtatc cactttgatg agggttcgag
    25381 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    25441 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    25501 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    25561 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    25621 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    25681 ggcagatatt caggcagtaa cgcccgataa aacggtacgg ataaaaacgc tcgtgcaagg
    25741 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    25801 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    25861 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    25921 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    25981 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    26041 cgaactgggt ggtgcccgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    26101 tacctctgcc tgacattacg cgcacgatgg tcaagtcatc accactgtgc tataaaataa
    26161 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    26221 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    26281 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    26341 tccgcttaat cgtattgagg tgaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    26401 gcgtcatttc cgccacttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    26461 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    26521 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    26581 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    26641 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    26701 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    26761 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    26821 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    26881 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    26941 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    27001 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    27061 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    27121 cccggctttc atactgccga ccatctgttc tggccgtacc cagcggtcaa actcggcttc
    27181 gctaagatac cccagcgcaa gggccgccgc ttttaaggtc agcccttctt tatgcgcttt
    27241 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    27301 cagcgattca ttgagtaatt gattgattcg ctcacgatta ggttcaatgc ccactgcgca
    27361 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattatg
    27421 gatcaccatc ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    27481 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    27541 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    27601 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    27661 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgccagcgc
    27721 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    27781 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccaag
    27841 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    27901 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcatcct gcaagtgggt
    27961 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    28021 ggttttaagc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    28081 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    28141 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    28201 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    28261 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    28321 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    28381 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    28441 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    28501 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgct
    28561 ttcgttaagc aacttttagc tcactcatta tttactattt gataacaaat gtttggtctt
    28621 tcgtgccatg taaaaaaccg ccccgaaggg cggctctgtt tatttcacac agcgggtgca
    28681 ttgtgtgagt tgtatctgct ggaagaagtc atttccttta tcatccacaa ggataaacgc
    28741 cgggaaatct tccacttcaa ttttccagat ggcttccatt cccagttccg gatattcaac
    28801 acattccagg ctcttaatac ttccctgcgc caatacagcg gccggaccac cgatactgcc
    28861 aaggtagaag ccgccgtgtt ttttacaggc atccgtcacc tgctggctgc ggttgccttt
    28921 cgccagcatg atcatacttc cgccctgcgc ttgcagttga tcgacataag aatccatccg
    28981 tccggcggtc gttgggccaa gagaaccgga ggcataacct tccggcgttt tggccggacc
    29041 cgcgtagtaa atcggatgat ctttaatgta ctgcggcagc ccttcgccat tatccatccg
    29101 ctctttcagc ttggcgtggg caatatcacg accgacgata atcgtgccat tcagcgacaa
    29161 gcgcgtagaa acgggatact gcgacaactg tgcgaggatc tctttcatcg gacggttaag
    29221 gtcaacgcgc accgcttcgc cttctcccgc tttacgcagc tcttcaggga tatatttacc
    29281 tggattatgt tccagttttt cgatccagat cccctgacgg ttgatcttcg ctttgatatt
    29341 acggtcagca gagcaggaaa cacccatacc aaccggacag gatgcgccgt gacgtggcag
    29401 gcgaatcacg cggatgtcgt gagcgaagta tttaccacca aactgcgcac ccagaccgag
    29461 attttgcgct tcgatcagca attctttttc cagttccaca tcgcggaacg cctgaccgtg
    29521 ctcattccct tccgttggca gttcatcata gtatttcgcg gaagccagtt tcaccgtttt
    29581 aaggttcgtt tctgcagaag ttccaccaat aacgaacgca atatgatacg gaggacaggc
    29641 cgccgtaccc agcgtgcgca tcttctcaac caggtaattt ttcagtttcc ccggcgttag
    29701 taacgctttg gtttcctgat agagatacgt cttgtttgcc gaaccaccac ctttggcgat
    29761 acagaggaat ttgtactcgt cgccatcaac ggcataaaga tcgatctgcg ctggtagatt
    29821 ggtgccagta ttcacctctt tatacatatc cagcggcgcg ttttgcgagt agcgcagatt
    29881 atcttcgata taagtgttat agacaccgcg cgccagcgcc gcttcatcac caccaccggt
    29941 ccatacacgc tgcccttttt taccaacaat aatcgcagtg ccggtatcct gacaggttgg
    30001 cagaacacct ttcgccgcga tgtcggagtt gcgcaggaat tgcagcgcca catatttatc
    30061 attttcgctg gcttccgggt cacgcagaat gtcggccacc tgttgttggt gagccggacg
    30121 cagcataaat gacgcatcat gaaacgcctg acgcgccaac agagttaacg cttcgggtgc
    30181 gactttcaaa atctcctgcc cttcaaattc agatacgcta acgtgttcgc tggttagcag
    30241 gtaatactca gtatcatctt ttttgagtgg aaaaggagcc tgataatgaa agggtttgtt
    30301 tgacattgtt ctctcactta ctgcctggtt tggttatgct ctgggcgggt gttccgttgc
    30361 cctgttaaaa gcgagtaaca atatcctaca cactttttta acaaaaactg agactagtac
    30421 gactttttgc ggctccaggt tacttcccgt aggattcttg ctttaatagc gggattaatt
    30481 tccacattaa aacagggatt gatcatgcaa aaactcatta actcagtgca aaactatgcc
    30541 tggggcagca aaacggcgtt gactgaactt tatggtatgg aaaatccgtc cagccagccg
    30601 atggccgagc tgtggatggg cgcacatccg aaaagcagtt cacgagtgca gaatgccgct
    30661 ggagatatcg tttcactgcg tgatgtgatt gagagtgata aatcgactct gctcggagag
    30721 gccgttgcca aacgctttgg cgaactgcct ttcctgttca aagtattatg cgcagcacag
    30781 ccgctctcca ttcaggttca tccaaacaaa cacaattctg aaatcggttt tgccaaagaa
    30841 aatgccgcag gtatcccgat ggatgccgcc gagcgtaact ataaagatcc taaccacaag
    30901 ccggagctgg tttttgcgct gacgcctttc cttgcgatga acgcgtttcg tgagttttcc
    30961 gagattgtct ccctactcca gccagtcgca ggtgcacatc cggcaattgc tcacttttta
    31021 caacagccta atgccgaacg tttaagcgaa ctgttcgcca gcctgttgaa tatgcagggt
    31081 gaagaaaaat cccgcgcgct ggcgatttta aaatcggcgc tcgacagtca gcagggtgaa
    31141 ccgtggcaaa cgattcgttt aatttctgaa ttttacccgg aagacagcgg cctgttctcc
    31201 ccgctgttac tgaatgtggt gaaattgaat cctggcgaag cgatgttcct gttcgctgaa
    31261 acaccgcacg cttacctgca aggcgtggcg ctggaagtga tggcgaactc cgataacgtg
    31321 ctgcgtgcgg gtctgacgcc taagtacatt gatattccgg aactggttgc caatgtgaaa
    31381 ttcgaagcca aaccggctaa ccagttgttg acccagccgg tgaaacaagg tgcagaactg
    31441 gacttcccga ttccagtgga tgattttgcc ttctcgctgc atgaccttag tgataaagaa
    31501 accaccatta gccagcagag tgccgccatt ttgttctgcg tcgaaggcga tgcaacgttg
    31561 tggaaaggtt ctcagcagtt acagcttaaa ccgggtgaat cagcgtttat tgccgccaac
    31621 gaatcaccgg tgactgtcaa aggccacggc cgtttagcgc gtgtttacaa caagctgtaa
    31681 gagcttactg aaaaaattaa catctcttgc taagcttatt aaaggcttat aacaccttca
    31741 ggcggccagt ccgcctgatt tcattttatg gataatcatt atgaataaat cgctggtagc
    31801 ggtaggcgtc attgttgcgc taggcgtagt ctggacaggc ggcgcatggt atacaggcaa
    31861 gaagattgaa acccatctcg aagacatggt cgcgcaggcg aacgcgcaac tcaaactgac
    31921 agctcctgaa tccaacctgg aagtgagtta tcaaaactat catcgcggcg tattcagcag
    31981 ccagttgcaa ctgttggtga aacccattgc cgggaaagaa aatccgtgga ttaaaagcgg
    32041 tcagagcgtc atcttcaacg aatcggttga tcatggtccc ttcccgcttg cccagcttaa
    32101 aaaactgaac ctgatcccgt cgatggcatc aattcaaacc acgctggtta ataacgaagt
    32161 aagcaaacca ctgtttgata tggcaaaagg tgaaacgcct tttgagatta actcgcgcat
    32221 tggttacagc ggtgattcca gttccgatat ttcgctcaag ccactgaatt acgagcaaaa
    32281 ggatgaaaaa gtcgccttta gcggcggcga gttccagtta aatgcggaca gagacggcaa
    32341 agctatctcc ctttccgggg aggcgcaaag tggtcggata gacgcagtta acgaatacaa
    32401 ccagaaagtg cagttgacct ttaataatct gaaaaccgac ggttccagca cgctggcaag
    32461 ttttggtgag cgcgtaggaa accaaaaact gtcactggaa aaaatgacca tttcggtgga
    32521 aggcaaagaa ctggcactgc tggaaggcat ggagatcagc ggtaaatcgg atctggtcaa
    32581 tgatggtaaa acgatcaata gccaactgga ttactcgcta aacagcctga aggtacagaa
    32641 tcaggatctg ggcagcggca agctgacttt aaaagtcggc cagattgatg gtgaagcctg
    32701 gcatcagttt agccagcaat ataacgcgca aactcaggcg ctgctggcac agccagaaat
    32761 tgccaacaat cccgaacttt atcaggagaa agtgacggaa gccttcttta gcgccctgcc
    32821 gctgatgttg aaaggcgatc cggtgattac tatcgcgccg ctaagctgga aaaacagtca
    32881 gggtgaaagt gcgctgaatc tgtcgctgtt cctgaaagat ccggcaacga ctaaagaagc
    32941 gccgcaaacg ctggcgcagg aagtagatcg ttcggttaaa tctctggatg cgaaactgac
    33001 cattccggtg gatatggcaa ctgagtttat gactcaggta gcgaagctgg aaggttatca
    33061 ggaagatcaa gcgaaaaaac tggcgaaaca gcaagttgaa ggtgcatcag caatggggca
    33121 gatgttccgt ctgaccacct tgcaggacaa taccatcacc accagcctgc aatatactaa
    33181 cggtcagata acgttaaacg ggcagaaaat gccactggaa gatttcgttg gtatgtttgc
    33241 aatgccggca ttaaatgttc cggtcgtacc cgctattccg cagcagtaat tcacagccaa
    33301 aacgccggag atttttctct ccggcgttat tttttacttc agcataaagt catactgaat
    33361 actcattttc acatcccagt aattcaggcg atcgcccagc gtactgccat cgcgcttttc
    33421 ccgcccttta tcggcagcaa gaaaaaaatt catcccttgt aacggccctt cgctgacccg
    33481 atagttcata ccacctaaga tacccgcgcc ttcaatccgc acatctcgtt tactggattc
    33541 atagccgcga gtcacaatcg gtgcaaatat cagtgtaaat tgcggcgtta aacgatagtt
    33601 gacgcccaat tgccaggact gcattccttc acggttacga tcaagactta atgaaaaggg
    33661 atagccaatg tcggtatcca ccacggcacc attcattgca tggcgcaaat aaccaccact
    33721 gccaaatacg cttccccacg gcgcattcca ggtcaattgt ccactaacca acgccgtttc
    33781 attgggctgg ctggtattac gacttaaacc ttcgagctgc gcgtaaaagc cgagcaactc
    33841 ccctttcaac gtataattcg ggtcgagcgg tatgcttttc tcgagaatac tttgtatctg
    33901 attggaaaca tctttctgta ccgatgcccc cagagaatag cgactggtat catcatgcca
    33961 actggcggct aaaaaagaac gtgcgcgatg tttatcgtat tttcccgtct ggctgttata
    34021 caccccttca tcacgaatac cggttgttga atcagtccat aaaaatactc gtgaacgcca
    34081 cattccccac agatcaatat ttggtgttaa agcccaataa cctaatgctc cttcagaaac
    34141 tgtcggtaat gtacgttgcg aactgccagc cattaacgga atacgataag gtaagctacc
    34201 ataactataa tccgtcccga agcgacctaa tttaagcagg ctattttcac tgggttttat
    34261 ttttactgcc cccagggcaa agccaaaact tttatcacca tcaagatacc accgggtact
    34321 catatcagca cgagcaccta atttataaac ataatacgcc ccgccttcaa cgccaataat
    34381 attggaataa taaccactat tgaaatcgag caatcctccc tgtacccatg cgtaaatatc
    34441 tcttcccgcg ccagcactgg gcttatccgc cctgcgcaat tcgtttttca gtctgatgcg
    34501 tagtcccgct tcatcgtcag ccagttgcgc cgcataagca gaggtaaggc cagaggcagc
    34561 cgtc
//
LOCUS       DAPPNJ010000024        34323 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000024 DAPPNJ010000000
VERSION     DAPPNJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.024"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            55..216
                     /locus_tag="RPM73_000910"
     CDS             55..216
                     /locus_tag="RPM73_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519300.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(300..1535)
                     /locus_tag="RPM73_000911"
     CDS             complement(300..1535)
                     /locus_tag="RPM73_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDX6519301.1"
                     /translation="MLQQIPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKSVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALKLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1682..1758)
                     /locus_tag="RPM73_000912"
     tRNA            complement(1682..1758)
                     /locus_tag="RPM73_000912"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1722..1724),aa:Pro,seq:tgg)
     gene            complement(1801..1887)
                     /locus_tag="RPM73_000913"
     tRNA            complement(1801..1887)
                     /locus_tag="RPM73_000913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1851..1853),aa:Leu,seq:cag)
     gene            complement(1908..1983)
                     /locus_tag="RPM73_000914"
     tRNA            complement(1908..1983)
                     /locus_tag="RPM73_000914"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1948..1950),aa:His,seq:gtg)
     gene            complement(2042..2118)
                     /locus_tag="RPM73_000915"
     tRNA            complement(2042..2118)
                     /locus_tag="RPM73_000915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2082..2084),aa:Arg,seq:ccg)
     gene            complement(2221..3606)
                     /locus_tag="RPM73_000916"
     CDS             complement(2221..3606)
                     /locus_tag="RPM73_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6519302.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKVHKLEE"
     gene            complement(3797..4537)
                     /gene="rffM"
                     /locus_tag="RPM73_000917"
     CDS             complement(3797..4537)
                     /gene="rffM"
                     /locus_tag="RPM73_000917"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDX6519303.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4540..5892)
                     /gene="wzyE"
                     /locus_tag="RPM73_000918"
     CDS             complement(4540..5892)
                     /gene="wzyE"
                     /locus_tag="RPM73_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDX6519304.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5889..6968)
                     /gene="rffT"
                     /locus_tag="RPM73_000919"
                     /gene_synonym="wecF"
     CDS             complement(5889..6968)
                     /gene="rffT"
                     /locus_tag="RPM73_000919"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDX6519305.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6965..8215)
                     /gene="wzxE"
                     /locus_tag="RPM73_000920"
     CDS             complement(6965..8215)
                     /gene="wzxE"
                     /locus_tag="RPM73_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDX6519306.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAVASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8217..9347)
                     /gene="rffA"
                     /locus_tag="RPM73_000921"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8217..9347)
                     /gene="rffA"
                     /locus_tag="RPM73_000921"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDX6519307.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9352..10026)
                     /gene="rffC"
                     /locus_tag="RPM73_000922"
                     /gene_synonym="wecD"
     CDS             complement(9352..10026)
                     /gene="rffC"
                     /locus_tag="RPM73_000922"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDX6519308.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10004..10885)
                     /gene="rfbA"
                     /locus_tag="RPM73_000923"
     CDS             complement(10004..10885)
                     /gene="rfbA"
                     /locus_tag="RPM73_000923"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDX6519309.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10904..11971)
                     /gene="rffG"
                     /locus_tag="RPM73_000924"
     CDS             complement(10904..11971)
                     /gene="rffG"
                     /locus_tag="RPM73_000924"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDX6519310.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11971..13233)
                     /gene="wecC"
                     /locus_tag="RPM73_000925"
     CDS             complement(11971..13233)
                     /gene="wecC"
                     /locus_tag="RPM73_000925"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDX6519311.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13230..14360)
                     /gene="wecB"
                     /locus_tag="RPM73_000926"
     CDS             complement(13230..14360)
                     /gene="wecB"
                     /locus_tag="RPM73_000926"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDX6519312.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLIDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14416..15462)
                     /gene="wzzE"
                     /locus_tag="RPM73_000927"
     CDS             complement(14416..15462)
                     /gene="wzzE"
                     /locus_tag="RPM73_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDX6519313.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15474..16577)
                     /gene="wecA"
                     /locus_tag="RPM73_000928"
     CDS             complement(15474..16577)
                     /gene="wecA"
                     /locus_tag="RPM73_000928"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDX6519314.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16817..18076)
                     /gene="rho"
                     /locus_tag="RPM73_000929"
     CDS             complement(16817..18076)
                     /gene="rho"
                     /locus_tag="RPM73_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDX6519315.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18161..18262)
                     /gene="rhoL"
                     /locus_tag="RPM73_000930"
     CDS             complement(18161..18262)
                     /gene="rhoL"
                     /locus_tag="RPM73_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDX6519316.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18403..18732)
                     /gene="trxA"
                     /locus_tag="RPM73_000931"
     CDS             complement(18403..18732)
                     /gene="trxA"
                     /locus_tag="RPM73_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDX6519317.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18863..20128
                     /gene="rhlB"
                     /locus_tag="RPM73_000932"
     CDS             18863..20128
                     /gene="rhlB"
                     /locus_tag="RPM73_000932"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDX6519318.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20262..21746
                     /gene="gppA"
                     /locus_tag="RPM73_000933"
     CDS             20262..21746
                     /gene="gppA"
                     /locus_tag="RPM73_000933"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDX6519319.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21793..23814)
                     /gene="rep"
                     /locus_tag="RPM73_000934"
     CDS             complement(21793..23814)
                     /gene="rep"
                     /locus_tag="RPM73_000934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDX6519320.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24031..24480
                     /locus_tag="RPM73_000935"
     CDS             24031..24480
                     /locus_tag="RPM73_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDX6519321.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVNNGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24679..24960
                     /gene="ppiC"
                     /locus_tag="RPM73_000936"
     CDS             24679..24960
                     /gene="ppiC"
                     /locus_tag="RPM73_000936"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDX6519322.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25047..26522)
                     /gene="ilvC"
                     /locus_tag="RPM73_000937"
     CDS             complement(25047..26522)
                     /gene="ilvC"
                     /locus_tag="RPM73_000937"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDX6519323.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26672..27565
                     /gene="ilvY"
                     /locus_tag="RPM73_000938"
     CDS             26672..27565
                     /gene="ilvY"
                     /locus_tag="RPM73_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDX6519324.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27617..29161)
                     /gene="ilvA"
                     /locus_tag="RPM73_000939"
     CDS             complement(27617..29161)
                     /gene="ilvA"
                     /locus_tag="RPM73_000939"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDX6519325.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29164..31014)
                     /gene="ilvD"
                     /locus_tag="RPM73_000940"
     CDS             complement(29164..31014)
                     /gene="ilvD"
                     /locus_tag="RPM73_000940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDX6519326.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIEFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31079..32008)
                     /gene="ilvE"
                     /locus_tag="RPM73_000941"
     CDS             complement(31079..32008)
                     /gene="ilvE"
                     /locus_tag="RPM73_000941"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDX6519327.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32028..32291)
                     /gene="ilvM"
                     /locus_tag="RPM73_000942"
     CDS             complement(32028..32291)
                     /gene="ilvM"
                     /locus_tag="RPM73_000942"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDX6519328.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32288..33934)
                     /locus_tag="RPM73_000943"
     CDS             complement(32288..33934)
                     /locus_tag="RPM73_000943"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDX6519329.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33937..33987)
                     /locus_tag="RPM73_000944"
     CDS             complement(33937..33987)
                     /locus_tag="RPM73_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519330.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34074..34172)
                     /gene="ilvL"
                     /locus_tag="RPM73_000945"
     CDS             complement(34074..34172)
                     /gene="ilvL"
                     /locus_tag="RPM73_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDX6519331.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8304 a         9455 c         8682 g         7882 t
ORIGIN      
        1 tatcaatgct taattacgtc atcattttga tgacatgaaa ccggagcatt ttgcatggaa
       61 ttttcgtttt cacccaaact tcttgttgtt gctgtcgccg ccgctcttcc tctcatggcc
      121 agcgcagcag ataccccgtc aactgccacc gcacgcaaag gctttgccgg atacgatcac
      181 ccaaaccagt atccacagaa cgctgcaatt tattgaattt gcacgatcat gtaggccgga
      241 taaggcgttt acgccgcatc cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt
      301 tacttattca ccaccagcaa atgtgcctgc ataatgtcac tggccgggcg accgtgcgcc
      361 agcaaatcag ccattgcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg
      421 gcacacaaat aattcagccc cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg
      481 cagcctcccc agcacgcttt taacacgtta caactgcgac actgcgccgg taattgctta
      541 aatttatctt caccaaacac ctgctgttgc ggggaatcga tcatttctgc aattgtttgc
      601 tgatgcatat tccccagccg atattgcgga taaacatagt gatcgcaggc gtaaacgtcg
      661 ccgttgtgct caacaatcac cgagcgccca caggttggct gatgatggca aaccgcaccc
      721 ggcgcaccga caaaattggc aaacgcccat tcgatattca tcacgaaaat cttgccgacg
      781 tcgcgtttga tccagtggtc gaatatcgcc accagaaatt caccaaattc atcggggcgc
      841 accgaccatt ccgtcagttc cccctgaata tcaccaggcg catgtagttt cagtccatcg
      901 ctggcagctg tttcatcagc caggcgctcg accaccggaa taaactggat gaattcgact
      961 cccgcatcgc acaaaaaatc ataaacctgc aacggttgct gcgcgctggt gcgattaacg
     1021 cagaccagca cgttatagtc gacatgatgc ttttgcagca gcttcaaggc acgcatcacc
     1081 agtttatgcg tgggtctgcc gcctttggtc acgcgatatt gattgtggat ctcagccgga
     1141 ccatccagcg ataacccaac aagaaaatga ttttctgcca gaaatgcaca ccattcatca
     1201 tcgagcagca cgccgttagt ctggaagctg ttacttatct tcctgccagc accatatttc
     1261 gcctgtagag ccacggcacg gcggtaaaac tccaacccga gcaacgttgg ttcgccgccc
     1321 tgccaagtaa aagccacttc gttttgtggt tcgctggcag cgatatagtg acggacatac
     1381 gcttccagcg tgtcatcgtc catatgcgtg actgactttt cgcggtaaag ggattgtttt
     1441 tcgagataaa aacagtagtc acagttcaga ttacaatcgg aaccgctcgg tttcgccatc
     1501 acatgaaaag cacgcgttgg aatctgttgc agcatggtcg ctccttgcct gaagtcatcc
     1561 ttcaatacgt taagggcgaa atcataccgt ggggcaatga gttattacgg gcgtttattg
     1621 cgaagggaga gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa
     1681 ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag ttgcgctacc
     1741 aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc tttaaagtcg
     1801 tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac ctgaagctag
     1861 cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg ggtggctaat
     1921 gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact gagctatagc
     1981 caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg ccgggactaa
     2041 atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca gcgctctatc
     2101 cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac ttcccacccc
     2161 tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc gttttgctgc
     2221 ttattcctcc agtttatgca ctttaccgtg gcgattaagg ccaaaaactt tataaatcgc
     2281 cgtcaccgcc agcataaaga tgatgccgac aaacagcgac atacgcgtat cttcattaaa
     2341 gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg cccacgggaa
     2401 caggattgag cggaacggat ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag
     2461 ctggcttatc aatatcacaa accatggcac catccccgga agcacgctgg cactgtagac
     2521 gtagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc caattagcag
     2581 aatagcaata gatactgcca cacccgcaac tggtacgccg tgacgggaaa ctttcgccat
     2641 cgccgccggt aactgacggt ttttcgccag tgcgtagagc atacgtccgc aactgtacat
     2701 gccgctgtta cagccagaga gcgcagccgt cagcaccaca aagttgataa tgcccgccgc
     2761 tgcggtaata ccgattttgg caaaagtcag tacgaacggg ctgccgttgc tgccaatttc
     2821 attccacggg aagatggtga cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg
     2881 ccacagcact ttgcctacgg cactgcgcag cgtcacctgc ggattcttcg cttcaccggc
     2941 agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca gcgcggtcag
     3001 gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc taaaaccaat
     3061 cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga caatcatcac
     3121 gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac
     3181 cgccgccaga ttcgccaacg ccaccagcgc cactgcgatc aatgcgggta tccactgcgc
     3241 catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg agatccccac
     3301 cgccatccac ataaaccagt aagaccaggc agtgagatag ccaaagaacg ggctcatata
     3361 acgatgcgca taaacggcga acgaaccggt aaccggttcg aggaacaaca tttcgcccat
     3421 tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca atacggatgg
     3481 cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa tggtgccccc
     3541 cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg gtttgttatc
     3601 tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta tcgttatggg
     3661 tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa
     3721 atttgaatgc tttgtgtaat aaaaaagcag gcaggcgacg aagtgaccac tccgtcgctt
     3781 tacaaagaga ggaaaatcag aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac
     3841 gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac tccagtccca
     3901 gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc
     3961 caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg aagatctcct
     4021 gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga atgcgttcaa
     4081 acagcgcctg acgctgctcg ggtttaaaat aaccatcctg actgccaacg atattcacat
     4141 tccattggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta ccgcccacaa
     4201 gaaataccgg cgtcccttct ttgcctgcgc gcgccatcag ctcttcccag agatcggcac
     4261 cggcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt acaacgctga
     4321 tgccatccgc atatttaaat tcggcagcgt taattaactc cctgacctcg gcgttatctt
     4381 caatagtcag cattttttca gcattaatgg caaccagcgt tccctgctta agctgcccgt
     4441 cagcaaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac tgtaagccac
     4501 gcagcgtata ggtcggtgcc gtggtgttgt tattcattgt tatccttcaa cctgcgtccg
     4561 gagcgatgat tttgtacgtt tatgaatgag tccggcgctt tcaaaaagcc agtacaacag
     4621 ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagactacgc gtgagacaaa
     4681 cgaatccagc ccttcacgtg ccagcacgat catattgaag atcgccccaa agcagaaact
     4741 gtgcaatatc gcagccttat agcgattagt ctcgcggttg cccagctcat acagccagtc
     4801 gaaccatttg atgatcagtc caaccacaat cgccccgagt gggatgaaca acgcgccgcc
     4861 catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt ggttattcag
     4921 cacttcccag gtaaagtagt tggctgagtt cagcaccata ctcgggcgac ccggccacag
     4981 ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct ggaagtcgat
     5041 gttgtcgtag ttctgcaaca gcaacgccag attctcccat ggcgagaagg tgtcgcgagt
     5101 gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat agcgttttag
     5161 cgccagccag aacatgccca caatccccag cacgcccgcc gccgccagca tccacaacga
     5221 aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga tgatattggc
     5281 gcgagtgccg ccgacaatca tataagtcag caagccaaag gcgaccgtgc tgacgaggaa
     5341 aaacagccac gctttgctat cctggcgcag aaagtagacc accagcatcg ccgggatgaa
     5401 aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggagaaga tctgactgta
     5461 ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga cgcttaccag
     5521 cgcgatgccc atcaggatca cccacgtaag attggtctcc acgcggttca tggtaaacag
     5581 cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg tgacatagta
     5641 aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac
     5701 accaacatca aagcgaaata ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag
     5761 caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa actcaaacca
     5821 ggtcagcgtg gcaataaaca gcgtgcagag cagccagaca acaaacaggc cactgaattg
     5881 cagcagactc atgcgacctc cccggcggca atcgccaacg cccgctgcca gccttgtaga
     5941 tagttagggc taaagaaggc aatggtgttt ttatccaccg acgccaactg gcgctgcgct
     6001 tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcaccggcaa atgctgttcc
     6061 gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc cgcctgaatc
     6121 agtaagcaaa gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc aagatcgcac
     6181 tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcaaaat ttgcagattt
     6241 tcttcgctga ataactccag ccccgcctga cgaacttcct caatgtacgc ttcgttatta
     6301 ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg atgaacggcg
     6361 cacaaggcag caatatggtc attgctgcgg tcaccggagt tccccaccag aatggtcatt
     6421 ttcccttcac gttgccgatc gttcgccatc gtattgagag aagggtccat ccgcgtcggg
     6481 aagtacagca gttcgccccg cacctttgga tgcgttttgg caaaaaagct caaatcaccg
     6541 cgggtggcaa atacacagcc gactcgcttt tgcgccaggc gacgtagcgg gtaaaaaagc
     6601 ttatatctca agccactgga aagctcatac aggtctgccc cccagatatg ccagaaaaac
     6661 tggctgggct taatgccacc actcagcaga gccagccaca gtttgggatt gaactgaccg
     6721 tggaagaaaa aacgctgctg acggttagct tttgctttcg cgatgaccgc ttccgccagc
     6781 gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact taagccgtcg
     6841 tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcattg
     6901 aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac gtgaatcagt
     6961 acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa aaatagacga
     7021 tataagttgc catataggcc tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt
     7081 gggcaaatac catcaataaa gtgaactggc tgacctccgc cagaatataa aaccgcagtg
     7141 acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac acatcaccca
     7201 ccaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac agcagccaga
     7261 tagcaaaatc acgcagcagc cagacggtaa aactcgccac cgccactgcc ggtaagacga
     7321 atttcagcga tttaaccact tcccgtgtga tatcgcgctt ttccgttagc cgcgacaacg
     7381 tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca tcggaaatac
     7441 tgctcacccc ttgccagatc cccacttcat cccagctata ctgcgccgcc agcagtttac
     7501 gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt
     7561 tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt aacgggatga
     7621 caccacgttt gatcaacatg atggcggcag gaatcaccac cagcgcggga atcagcgcca
     7681 gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac gcgagaacgc
     7741 caatcaagct gccgacaatc agcgataacg cattacctgc ggcatcgcga aagcctttca
     7801 tcagcgccag taacaggttg ccccaggcaa tccccatttg caccagcgcc actaaacgca
     7861 ccagcccctg atagtcggta ttaccaaaca atccctggct gattggcgca gctgccagca
     7921 caaaaaccag cgccatcagc gtagagaagc caagtaccat cgctgatgaa gtgccgacca
     7981 cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt acaccgttaa
     8041 agatgccagc cccggcaagc acgccgagca cggtaatcaa ctggcggaaa tttgccgcca
     8101 gcccaagccc cgcaggacca aatgacaccg ccagcaactt accgaccagt aacccggcac
     8161 caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac gacatatcag
     8221 gaaaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga caggttgtag
     8281 aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg
     8341 aactcaccaa agcgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc
     8401 atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt
     8461 ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg cagctcgata
     8521 cgcccggctt tcgccagagg cgctaacgca tcgtagtagt tttgccacag cgccagacgt
     8581 tgctggttga tacgatccgc tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg
     8641 gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg accacggaag
     8701 aactggctgc ggttagtgcc cttttcacgg atgatctcgg ctcgttcgat taacgcttta
     8761 tcgttaatca gcgtcgcgcc gccttcaccg cccgccgtgt agtttttggt ttcatggaag
     8821 ctaaagcagc caatatgacc aatggttccc agtgcacgcc ctttgtaagt ggacatcacg
     8881 ccctgagcgg catcttccac cacaaacaga ttatgctttt tcgccaacgc cataatggtg
     8941 tccatttcgc aggccacgcc cgcgtaatgc acaggcacga taacgcgcgt tttgtcggtg
     9001 atcgccgctt caatcagcgt ttcgtcgata ttcatggtgt ccgggcgaac atccacaaag
     9061 acgattttcg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt gtagctcggc
     9121 atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc cagcgaagcg
     9181 gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag ccactgctgg
     9241 cagcgacggg taaaaccgcc atcgccacac agtttgccgc tacccattgc cgactgcata
     9301 tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg atcacctgta
     9361 taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt taagcgcggc
     9421 ggtgttgccc atttgggtcg ccacccgcaa agttgtttta ccgcgagcat acgcccagtt
     9481 tagcgccgtt tgcatcagct cagcacctgc accgcgtcca gccagcaggc caattcgcgc
     9541 atctgtcgca ttgagttccc gtaaagagac atagccgcga atatcgccgg acgccgcacg
     9601 taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc actgtgcata
     9661 aaagcgactg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc
     9721 ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta cagcaccgct
     9781 atcactggca ttgttcacgg gtagcgccaa atcaacttca ccttctacca gggagaatcc
     9841 cagctgttgc agggcatcca gttcacccgt atttgatgcc gcaattttgg cctgcacccg
     9901 tgaccacggc gctaacgcgt ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat
     9961 ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac tggcgcggac
    10021 gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat gaactggcag
    10081 cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcgatctct tccaggcagg
    10141 caatcttaaa gccctggcgt ttttctactg tctgcacaaa ggtactggct tcaatcaggc
    10201 tgtcgtgagt gccagtgtcc agccaggcaa atccgcgccc gagcagttca acggtcaggt
    10261 tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca cgctccgacg
    10321 gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc ccggtcaccg
    10381 cccagtttga cttcggctgt tttggctttt cttccagcga gatagcgcgg aaattgtcgt
    10441 caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat accgtcgccc
    10501 cttccgtgcg cgccgcaaca tgacgcagct tcggactgaa gccctgaccg aagaagatgt
    10561 tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg atgataaagg
    10621 cctgcgccag accgtccggg ctgggctgtt cggcgtattc cagctggata ccgaactcac
    10681 taccatcgcc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg atgatgagaa
    10741 tttcgcggat accggccaac atcagcaccg acagcggata gtaaatcatt ggcttatcgt
    10801 aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac
    10861 cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct ttcagaccta
    10921 aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca ttagccagat
    10981 accactgcac cgttttacgc attccacttt caaaggtttc ctgtggcagc caaccaagtt
    11041 cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga cggtcagcga
    11101 caaaggtgat caagtcacga tagtgcgcca cgccgtgcgg cttgttcgga gccagttctt
    11161 ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg tgaccaccaa
    11221 tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat
    11281 cttccacata cagccagtca cggatttgct gcccgttgcc atataccggc agcggtttac
    11341 ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg taaggaccgt
    11401 agttattcga gcagttggtg atcagcgtcg gcagaccgta ggtccgcagc caggcgcgca
    11461 ccagatggtc gctactggct tttgacgcgg aataagggct gctcggcgca tacggcgtgg
    11521 tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg gtggagatat
    11581 gatgaaaacg gaacgctgat tttttatctt ccgtcagcgt attccagtaa gcccgcgccg
    11641 cttcaagcaa tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa
    11701 tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc tgatgctcag
    11761 tgaatacgcg tgccagttct gcccgatcgc agatatcaac tttctcaaag gcaaagtgct
    11821 cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc ttatcgacca
    11881 ctaccaccgc atcgctcgtt tcgttgatga tataacgcac cagcgccgag ccaataaacc
    11941 cggcaccacc tgttatcaga atttttctca tcagcgccag actcctttgg catcgacgac
    12001 atactgctga tggacattgt cgccattgat aactttgaac tgactatgat cgaccagcat
    12061 caccagcacg tctgccgttg ccagcgcctc gtcaagctgc gccagagtac aaagcccggt
    12121 cagttttttc ggcaactggt ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca
    12181 ctgggcgatc agttcagcga tttccatcgc cgggctttcg cgcaggtcat caatattcgg
    12241 tttaaacgcc agaccaaagc aggcgatttt cagttcactg gcgcgtttat cggtagcagc
    12301 aaggcagtcg gcaaccgccg ctttcacctg atcgataacc cagaacggtt tgtgatcgtt
    12361 aacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca cgataaacca
    12421 cggatcaaca gcaatgcagt gaccgcccac gccagggcca ggctgaagaa tattaacgcg
    12481 agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat cggcacaaat
    12541 cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt cggtgagctt
    12601 acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga aaattttgta
    12661 cagttcgctg gcgcgggtcg aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt
    12721 aatcagctcg accataacct ggcccggtaa cacgcgttcc gggcagtaag caatgttgac
    12781 gtccgcctgc tcgcccacct gctgtgggaa agtgagatcc ggacgcatct ctgctaacca
    12841 ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca ccagcgcgcc
    12901 ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca tatctggctc
    12961 atgatcgcct ttaaacggcg taggtacagc aatcagccag gcatccgctt caactggcgt
    13021 cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg ccaaatcagg
    13081 ttcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt tgatatcgac
    13141 accaatcacc tgtttttgcc gtgaggcaaa cgcagctgct gtaggcagcc cgatgtaacc
    13201 cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt ttttaacgct
    13261 tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg gctcatagct
    13321 tgatattcgt tttcgtctat taaaagacgc gtcacttcct cgacaattcg ctgcttatcc
    13381 gtgcctacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt ggtatcgcgc
    13441 atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc tgagtcagtc
    13501 aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg gggatcgatc
    13561 agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac gttcgggttg
    13621 agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg
    13681 tggcagattt cttcaaagcc acgaccgaaa ctttcacgcc tgtgaccggt caccagaatc
    13741 atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg cagcgtgtcg
    13801 ctgctcatca cctgatcacg cacccataac agtgcatcaa tgactgtatt accagtaatg
    13861 aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt ttcggttgga
    13921 gagaagtgat acatcgccag atgcccggtc aatgtacggt tagcctcttc cggccacggc
    13981 gaatagagat cgcccgtgcg taaaccagcc tcaacgtgac caacaggaat acgctgataa
    14041 aacgccgcca ggctggttgc cagcgtcgtt gttgtgtcgc cgtgaaccag cacgacgtct
    14101 ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt tatctctgtc
    14161 aggccctgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga aaagagtttc
    14221 agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa
    14281 ggatcttttg ccaacgcatg caccaacgga gccatcttga tggcttccgg gcgcgtacca
    14341 aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc ctttgcgctc
    14401 gcagcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag ccccgatcag
    14461 ccccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat cgcgttttac
    14521 cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca gagttggacc
    14581 aacattcagg gtgtttaaca tggcccgatt ctgatcatag tcgagatcaa aggccggacc
    14641 gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca ggaacatttc
    14701 tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat tatgctgctc
    14761 agcaattttc agcgcctgct caatgctgtt catccggcgg tcgtagatgg ctttcgccac
    14821 ctcttcctga cgcttcacct gagctttcat ctggatggtg cgtgccgccc atgcgccttt
    14881 cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat actgacgtaa
    14941 caggttatta gcgtcaggcg cggtttcggc aataagcttc acgctgtcat tgaccgcgcg
    15001 ggtaaagtct ccggggataa actggatgtt gttaatcatt tcatccagca acgccgcatc
    15061 ggctttgctg ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc agaactctct
    15121 gcgggtatcc cacgaggcca gttgcataac aaactcttta taggcttcgt ccatgaccga
    15181 tggttgatcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa attgctgctg
    15241 cgagtaatat ccccccagca tattcaccgt tggacgatcg gtaatcgccg tcgagctcca
    15301 ctcctgacga gcaaaaaaag tatacgccag cgcgattaac gcaaacgcca gccccatgcc
    15361 aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat ccagttcatt
    15421 ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct catttatttg
    15481 gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg cttaataaag
    15541 cgagcaactt tccaggcacg cttaatgcaa tatccataga ggaagaatgc tagcaaaaag
    15601 agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac gccaatggaa
    15661 gcgagcagtg ctgcggcaag ggtaatcagc acaaacgcct gacgggaagt aaacccggca
    15721 cgcatgatca aatggtgaat atgctgacgg tcaggagaga atgggctcat gcctttacgc
    15781 aggcgacggt acataatcgc caccatatcc attagcggaa tggcgattat ccacaaagcg
    15841 gtaaccgggc tgatgggatg ggttttgccc tgggtcgttt cgagcaggat ccagataacg
    15901 gtaaaaccaa tcagcgtact gcccgcatca cccataaaga ctttgtagcg gcgacccagg
    15961 ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa gcaccagatt
    16021 gcgaggctgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa cgagacgcag
    16081 gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc
    16141 cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc ccaggagcca
    16201 aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac aatgccaaca
    16261 gcggcctgta tggtggcacg gatttttacg ctgatatcaa aacggtcatc cagcgcgcca
    16321 atgaaaacaa gcacaccggc acaagcgaga tagagagatg catgcggaat atagtaatcg
    16381 acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac gagaggtatc
    16441 aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt ttttgccact
    16501 ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag atcagtactc
    16561 actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa gtataaccac
    16621 gaagaccttt attcagaagg gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta
    16681 accatttgat atgcaaattg cataacatgc cagcaaattc caggaatttt cctaacatcg
    16741 ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt
    16801 ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca ttggtcttag
    16861 tcatggccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc gggtgaatga
    16921 ttttgcgcag gatccacatt ttctgcagtt cttcctgagt cgtgagcagc tcttctttac
    16981 gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg atcttacgag
    17041 agaggtgcag ttccatgttg cctgtacctt taaactcttc gtaaataact tcgtccattt
    17101 tagaaccggt atcgataagc gccgtcgcga taatggtcag gctgccgccc tcttccacgt
    17161 tacgcgccgc accgaagaag cgtttcggac gatgaagggc attggcgtcc acaccaccgg
    17221 tcaacacttt acctgacgcc ggaacaacag tgttgtaagc gcgcgccaga cgagtgatgg
    17281 agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttggcc ttctcgatca
    17341 ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt
    17401 caccttttac cagacgctgc atctcggtta cttcttccgg acgttcgtcg atcagcagaa
    17461 ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc tgcagcagca
    17521 tagttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga ccgataggtg
    17581 atgccagatc cagtacgcga gcggttaaat cttccgtaga accgttacca cgttccatac
    17641 gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg ttgcgggcgt
    17701 tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag cgttcacctt
    17761 ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg aaacggcgga
    17821 tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg tctgcggaac
    17881 ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag atatcttcgc
    17941 cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc atacgagcca
    18001 ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc gtattcttta
    18061 attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg
    18121 ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc atgtcttttc
    18181 gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg agtgacgagc
    18241 cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat tacaagattc
    18301 aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac tcacgccggg
    18361 cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca ggttagcgtc
    18421 gaggaactct ttcaactgac ctttagacag tgcacccact ttggttgccg ccacttcacc
    18481 gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg cagtgccagg
    18541 gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt cagcgatttc
    18601 atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga aatcgacgag
    18661 gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat
    18721 tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc gttgttggtg
    18781 taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt aaagcaatag
    18841 taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga agttttccga
    18901 cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac
    18961 gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc
    19021 gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt atcttctctc
    19081 tcatcctgcg attgccgatc gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac
    19141 gcgtgaactt gccgtgcaga tccatgccga cgcagaaccg ctggcggaag ctaccggcct
    19201 gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag
    19261 cggcgttgac attctgattg gcactacggg gcgtttaatt gactacgcca agcagaacca
    19321 cattaacctc ggtgccattc aggtggtggt actggacgaa gccgatcgca tgtacgatct
    19381 gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct
    19441 caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt tcgagcagat
    19501 gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc gtattaaaga
    19561 agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga
    19621 agagtggcca gacagagcga ttattttcgc caataccaaa caccgttgtg aagagatctg
    19681 gggccacctg gcggcagatg gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa
    19741 aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc tggttgccac
    19801 tgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg cacgtcttta actacgattt
    19861 acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg caggcgcaag
    19921 cggtcactct atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg ctattgagac
    19981 ctatattggt cactcaattc cggtaagcaa atacaatccg gacgcattga tgaccgatct
    20041 gccaaaaccg ctgcgcctca cgcgcccacg tacaggcaat ggtccgcgtc gtactggcgc
    20101 tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat actcaaaatc attcaaactg
    20161 tatcaagatg gcaagagaat gaatcccgat gagcatactg tagtaagtga ttcaggtgaa
    20221 agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    20281 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    20341 ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct gctggcctga
    20401 atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt
    20461 ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct acggcgacat
    20521 tacgtcttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc
    20581 cggtacaggt gatcagcggt gaagaggaag cacgtctgat ttatcagggc gttgctcaca
    20641 caactggcgg tgccgatcag cgcctggtgg tggatatagg cggtgccagt actgaactgg
    20701 taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc tgcgtcacct
    20761 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    20821 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    20881 tgtgcgttgg tgcttccggc accgtgcagg cgttacagga aatcatgatg gcacagggga
    20941 tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc attcattgcg
    21001 gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    21061 gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt atgaccctgg
    21121 cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc gtcgagcagg
    21181 atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat attgatcagg
    21241 cgcagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaag gaatggcatc
    21301 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa atcggcctga
    21361 gcgttgactt taaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc
    21421 ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac cagactaatc
    21481 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac
    21541 tctgccgttt actccgcctg gccatcattt ttgccagccg tcgccgtgac gatctcgtgc
    21601 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    21661 taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg cagagctatg
    21721 tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct gacgcatctt
    21781 ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    21841 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    21901 cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca ccagctcgcc
    21961 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    22021 gacataggcc agccgccgct cctcatcgat attatcttca tcgatgctgc tctggtgcgg
    22081 caaaaaccct tcttccatac cgaccatgta gacataagga aactccagcc cttttgacgc
    22141 gtgcagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac gctccatcat
    22201 gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcgct
    22261 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    22321 ggcggctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccgtggat
    22381 cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctggattt ctgccaacca
    22441 gtgagtaaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    22501 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac ccagcttttt
    22561 cagcgtcgcc gggccaatct ctctcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    22621 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    22681 gaaaaacgac gtaccaccag atattttgta tgggatgcgg ttttgcatca ggaatttttc
    22741 aaacacccgc gactgatggt taccgcgata aagaatggcg taatctttgt actgcgtttt
    22801 attgacgaag tgatgggcga tcagctcgcc agtaacgcgc tcggcttcat gttcctcgtt
    22861 attcgcgctt aaaactttta gctctgtgcc gtaacccagt tcggagaaca gacgcttttc
    22921 aaagacgtgc ggattattgg cgatcaggat gttcgccgct ttcaaaatac gtccggaaga
    22981 gcggtagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac tcagcagcac
    23041 caagttttgt ggacgtgcgc cgcgccagga gtagatcgac tggtcgtcat cgcccaccac
    23101 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc tggtgttggt
    23161 gtcctgatac tcatccacca gcagatagcg aattttgttc tgccagcgct cgcggacttc
    23221 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacatt
    23281 gcacgctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac ggtcccgctc
    23341 gccaatcgca ctggctgccg cctgggacgg tgttttgaga tcgttcttcc agttagagat
    23401 ggtcgaaatc aactgttgca ggagaacttt gtcatcttca atcagccctt cggtcaactc
    23461 tttgagcaac gccagctgat cggtatcgtc aaacagcgaa aagttcgcct tcatccccag
    23521 cgccgcatac tcacgcttga tgatatctag ccccagcgtg tggaaggttg agatcatcag
    23581 cccacgcgcc tctttgcgac ccagcgtctg tccgacacgc tctttcatct cgcgcgccgc
    23641 cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag
    23701 atgggcgatt ttattggtga tcacacgagt tttaccggaa cccgcgcccg ccagcaccag
    23761 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggtgt
    23821 attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg
    23881 tgtaagccgc taaccggttg gctgcggatg tttaaatcag agagagaaga ccgaaaccgg
    23941 agttcatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt gttattactt
    24001 tttcagagtt tactaaggag aggtgtactc gtggcgatta atttcagtcc taaggttggt
    24061 gaaatactgg aatgcaactt tggtaactat cctgttagcc agaatggaca gttcagcacc
    24121 aagtattacg atggccgcat tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc
    24181 aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat tatcaaccac tcgcgatcat
    24241 gacaagttaa atcgtggaat gcatgttgaa atagccagta atgttaacaa tggcctgcaa
    24301 ttctttgatc agcagattcg ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac
    24361 cgattaaata gagcaaggac ttacagaggg tatcttaatc aatgtttgcc acatgagttg
    24421 gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatatcgct cataaattga
    24481 caataaaata accatccccg ataatgactt tgtagcccat agaggcccta agttttctcc
    24541 ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt accagcccct
    24601 ccctgactga caattacgtt agcatctgcg gatcatgcta aaatcgccgc ctgacaatta
    24661 ttcactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa
    24721 gagaaactgg ctctggatct tctcgagcag attaagaacg gtgccgattt cggcaagctg
    24781 gcgaagaaac actctatttg cccgtcaggc aaacgcggcg gtgatttagg tgaattccgc
    24841 cagggtcaga tggttccggc gttcgataaa gtggttttct cttgtccggt actggagccg
    24901 accggcccgc tgcacaccca gttcggatat cacatcatta aggtgctgta ccgcaactaa
    24961 tagcaaggcc ttctccagga gaaggccttg agtgttttct ccctctccct gtgggagagg
    25021 gtcggggtga gggcatcagc gcgcacttaa cccgcaacag caatacgttt catatctgtc
    25081 atatagccgc gcagtttctt acctacctgc tcaatcgcat ggctgcgaat cgcttcgttc
    25141 acatcacgca gttgcgcgtt atctaccgcg ccttccggaa tagctttacc caagtcgccc
    25201 ggttgcagct ctgccataaa cggtttcagc aacggcacac aagcgtaaga gaacagatag
    25261 ttaccgtact cagcggtatc agagataacc acgttcattt cgtacagacg cttacgggcg
    25321 atggtgttgg caatcagcgg cagctcgtgc agtgattcat aatatgcaga ctcttcaatg
    25381 atgccggaat cgaccatggt ttcgaacgcc agttcaacgc ccgctttcac catcgcgatc
    25441 atcagtacgc ctttatcgaa gtactcctgc tcgccgattt tgccttcata ctgcggcgcg
    25501 gtttcgaagg cggttttgcc ggtctcttca cgccaggtca gcagtttctt atcatcgttg
    25561 gcccagtccg ccatcatacc ggaggagaat tcgccggaga tgatgtcgtc catatgtttc
    25621 tggaacaggg gtgccatgat ctctttcagc tgttcagaca gcgcgtaagc acgcagtttc
    25681 gccgggttag agagacggtc catcatcagg gtgatgccgc cctgcttcag cgcttcggtg
    25741 atggtttccc aaccgaactg aattagtttt tctgcgtatg ccggatcggt gccctcttcc
    25801 accagcttgt cgaagcacag cagcgagcca gcctgcaaca taccgcacag gatggtttgc
    25861 tcacccatca ggtcagattt cacttccgca acgaaggacg attccagcac gcccgcacgg
    25921 tgaccaccgg ttgctgccgc ccatgcttta gcgatcgcca taccttcgcc tttcggatcg
    25981 ttttccgggt gaacggcaat cagcgtcggt acgccgaatc cacgtttgta ctcttcacgc
    26041 acttcggtgc cagggcattt cggcgcaacc atgactacgg tgatatcttt acggatctgc
    26101 tcgcccactt cgacgatgtt gaaaccgtgc gagtaaccca gcgccgcgcc gtctttcatc
    26161 agtggctgta cggtgcgcac tacatcagag tgctgcttgt ccggcgtcag gttaaccacc
    26221 agatccgcct gcgggatcag ttcttcgtaa gtaccaactt taaaaccgtt ttcggtcgct
    26281 ttacgccagg acgcgcgctt ctcggcaatc gcttctttac gcagagcgta ggagatatcg
    26341 agaccagaat cacgcatgtt caggccctgg ttcagcccct gtgcgccaca gccgacgata
    26401 accacttttt taccctgaag gtagctcgcg ccgtcggcga attcgtcgcg ccccataaag
    26461 cgacatttgc ccagctgtgc cagctgctgg cgcaggttca gtgtattgaa gtagttagcc
    26521 atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc gagattgaat
    26581 tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc acattgcaat
    26641 ttctgcaacg tcaacattga gggctgcgcc attggattta cgcgatctga aaaccttcct
    26701 gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcgatgcacg ttagcccatc
    26761 cacgctctca cggcagattc agcgcctgga agaagatctt ggtcagccgc tatttgtgcg
    26821 cgataaccgc acggtaacgc tgaccgaagc gggcgaagag ttacgcgttt tcgcccagca
    26881 aacattgttg cagtatcagc aactgcgcca caccatcgat cagcaagggc cgtcgctctc
    26941 tggcgagtta catattttct gctcggtgac cgccgcttac agccatctac cgccgattct
    27001 cgaccgcttc cgcgcggaac acccgtcggt ggagatcaaa ctcaccactg gtgatgcggc
    27061 agatgcaatg gaaaaagtgg tcacgggaga agcggatctg gcgattgccg ggaaaccgga
    27121 gacgctaccc ggcgctgtgg cgttttcgat gctggagaat ctggcagtag tgctgattgc
    27181 ccccgcgctg ccctgcccgg tgcgtaatca ggtatctgtc gaaaagcccg actggtcaac
    27241 ggtgccgttt attatggccg atcaggggcc ggtacgccgc cgcattgaac tgtggtttcg
    27301 acgcaataaa atcagcaacc cgatgattta cgccacggtt ggcgggcatg aagcgatggt
    27361 atcgatggtg gcgctcggct gtggtgtggc gctgttgccg gaagtggtgc tggaaaacag
    27421 cccggagcct gtacgcaacc gggtaatgat tttagatcgc agcgatgaga aaacgccgtt
    27481 tgaactgggc gtgtgtgcac aaaaaaagcg gctgcatgag ccgctgattg aggcgttctg
    27541 gaggattttg ccgaaccaca aatgacgttg tcgcgcgggt aggcctgata agcgaagcgc
    27601 tatcaggcat ttttccctaa cccgccaaaa agaacctgaa cgccggatta ttggtttcgt
    27661 cgtggcaatc gtagcccagc tcatttagcc gggtttcgaa atccggttca tggtcgccga
    27721 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    27781 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    27841 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    27901 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    27961 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    28021 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    28081 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    28141 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    28201 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    28261 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcgcgca
    28321 catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg gtgatgatgt
    28381 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgctacgc
    28441 cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc agcgctgctt
    28501 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc atcagttgtt
    28561 tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    28621 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    28681 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    28741 tggctttcgc ttcatcaaag ttcgcgccgt ggagcagcac ttcgccgccg aagccgcgca
    28801 ccgcatcaac tttgatatcg gcggtggcgg ttggcatgac gatcagggcc ttcacgccta
    28861 accgtgcgga agaaaacgcg acgccctgcg cgtggttacc cgcagaagcg gtaatcacgc
    28921 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgcca cgaagcttaa
    28981 agctgtgcac tggctggcga tcttcgcgct tcaccagaat cacgttatcg agacgcgacg
    29041 acagtttttc cattttctgt agcggcgtga cctgcgccgc ttcgtagacc ggcgcgcgca
    29101 gcaccgctct taaatattcg gcaccttccg gggtaccgga caggggttgc gagtcagcca
    29161 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    29221 gctggcgtag gcgcgcaacg caaaggagac ctgacgttcg cgattttttg gcgtccaggc
    29281 tttgtcgcca cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    29341 taactgaatg ccacggttcg gaatgtcgat agcaatcatg tcgccgtctt caatcagacc
    29401 aatgctgccg ccgcttgccg cttccggcga gacgtggccg atggaaagac cagaggtgcc
    29461 gccggagaag cgtccgtcgg tgatcagcgc gcaggctttg ccgaggccca tcgatttcag
    29521 gaagctggtt gggtagagca tttcctgcat gccggggccg cctttcgggc cttcgtaacg
    29581 gatcaccacc acatcgccag cgacaacttt gccgccgaga atcgcttcta ccgcgtcgtc
    29641 ctggctttcg tacactttcg ccggaccggt gaatttgagg atgctgtcat cgacgcccgc
    29701 ggttttaacg atgcagccgt tttctgcgaa attaccgtag agcaccgcaa ggccaccgtc
    29761 tttgctgtag gcgtgttcca gcgagcggat gcagccgttt gcgcgatcgt catcgagaga
    29821 atcccaacgg caatcctgcg agaatgcctg tgtggtacga atgcccgccg ggccagcgcg
    29881 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagcgtttg
    29941 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cacgatccaa
    30001 ctcgccgaga ataccgataa caccaccagc acgatgaaca tcttccatat ggtacttctg
    30061 cgtactcggc gcgactttac acagctgcgg taccttgcgg gagagcttat cgatatcact
    30121 catggtgaat tcgatttccg cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt
    30181 agagccgccc atcgcgatat ccagcgtcat ggcattttca aacgccgcct tactggcgat
    30241 attacgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    30301 gcgcttacca gcattgagga acagttgctt gcgatcggcg tgggtcgcca gcagcgagcc
    30361 gttgcccggc tgcgacaggc ccagtgcttc ggtcaggcaa ttcatagagt tagcggtaaa
    30421 catcccggag caagatccgc aggttggaca ggcggaacgt tcaacctgat cgctctggga
    30481 gtcagaaact ttcgggtctg cgccctggat catcgcatca actagatcga gcttaatgat
    30541 ctgatcggac agtttggttt tcccggcttc catcgggccg ccggaaacaa agatcaccgg
    30601 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    30661 gcagaccatg gcgtcggcgc agtgagcgtt gaccatatac tcaacggaat cagcgatcag
    30721 ttcgcgagat ggcagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    30781 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt ctgcgaccag
    30841 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    30901 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    30961 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    31021 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    31081 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    31141 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca acctgaatac
    31201 catctacact gcgcactggc gtgatttctg ccgcagtacc ggacataaac acttcatccg
    31261 ccaggtacag ggattcgcgc gacagaacct gctcacgcac ttcaattccc agctctttcg
    31321 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    31381 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    31441 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    31501 gcagggaaga gagatagtta ccaccggctt ttgccgcggt tgggatggtg tttggtgctg
    31561 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    31621 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    31681 cacccatgcc aacatcacca acgaagatca gcggacggat ataggcgctg gtgagattgt
    31741 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc gaaaccggga
    31801 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    31861 ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaagtg ccatagtgca
    31921 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    31981 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    32041 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    32101 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    32161 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    32221 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    32281 tgttgcatca tgataatttc tccaacattt ctgaattact ggcgccaggc ggcaccagcg
    32341 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    32401 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    32461 cgaaggcgct ggctaacatg aggaaatcgg ggttatcagt aagggtggtt tcgctgtatc
    32521 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    32581 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    32641 tgaaagagcc gtcaccggag atacagacga cagtatcgtt cggtcgtgcg acttgtgcgc
    32701 caaccgccgc tggtaaaccg aaacccatgg tgcctaagcc gctggaggta atgaaatttt
    32761 ccgggcgagt gtgtgcgatg tgctgcgcgg cccacatctg gtgctgcccc acatctgtgg
    32821 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggcgcgtaga
    32881 tagcgtcacc gggatggtcg taacgccagg tatgttcatc acgcagctgc gcgcagtatt
    32941 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    33001 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    33061 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    33121 gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc gctttggtgc
    33181 catgcattcc cagcatgccc aggtagtacg gataatctgc ttcaactgcg cccagccctt
    33241 tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa gcaggaactg
    33301 cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct tttgccagca
    33361 tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    33421 accacggttc caggtcaccg ctggctagct ggatatcttt tgggatatcg accagaaccg
    33481 gaccaggacg acctgagctg gcaacgtcga atgctgcagc catgatgcgc ggcaactctt
    33541 ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    33601 cttcctgaaa tgcgtcggtg ccgataaaag gtgcggacac ttgaccggtg atggcaacga
    33661 cagggatgga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    33721 cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg atagccgcca
    33781 ttgccgcacc ctgctcatgt cggcatagca agtgctccac gccgccgtca tacaatgcat
    33841 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    33901 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    33961 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    34021 aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc
    34081 tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg
    34141 accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag
    34201 gaatgcctaa agagttacca tagattttgc gaatagcaca agatattttt gggcctattt
    34261 ttatgatggc tttaataatt tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt
    34321 gaa
//
LOCUS       DAPPNJ010000025        33469 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000025 DAPPNJ010000000
VERSION     DAPPNJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.025"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            303..389
                     /gene="leuL"
                     /locus_tag="RPM73_000946"
     CDS             303..389
                     /gene="leuL"
                     /locus_tag="RPM73_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDX6519332.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            482..2053
                     /gene="leuA"
                     /locus_tag="RPM73_000947"
     CDS             482..2053
                     /gene="leuA"
                     /locus_tag="RPM73_000947"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDX6519333.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2053..3144
                     /gene="leuB"
                     /locus_tag="RPM73_000948"
     CDS             2053..3144
                     /gene="leuB"
                     /locus_tag="RPM73_000948"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDX6519334.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3147..4547
                     /gene="leuC"
                     /locus_tag="RPM73_000949"
     CDS             3147..4547
                     /gene="leuC"
                     /locus_tag="RPM73_000949"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDX6519335.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4558..5163
                     /gene="leuD"
                     /locus_tag="RPM73_000950"
     CDS             4558..5163
                     /gene="leuD"
                     /locus_tag="RPM73_000950"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDX6519336.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5212..6390)
                     /gene="setA"
                     /locus_tag="RPM73_000951"
     CDS             complement(5212..6390)
                     /gene="setA"
                     /locus_tag="RPM73_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDX6519337.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSDVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="RPM73_000952"
     CDS             complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="RPM73_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDX6519338.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6712..8367
                     /gene="sgrR"
                     /locus_tag="RPM73_000953"
     CDS             6712..8367
                     /gene="sgrR"
                     /locus_tag="RPM73_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDX6519339.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8401..8500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8531..9514
                     /gene="thiB"
                     /locus_tag="RPM73_000954"
     CDS             8531..9514
                     /gene="thiB"
                     /locus_tag="RPM73_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDX6519340.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAANWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9490..11100
                     /gene="thiP"
                     /locus_tag="RPM73_000955"
     CDS             9490..11100
                     /gene="thiP"
                     /locus_tag="RPM73_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDX6519341.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLCHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11084..11782
                     /gene="thiQ"
                     /locus_tag="RPM73_000956"
     CDS             11084..11782
                     /gene="thiQ"
                     /locus_tag="RPM73_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDX6519342.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11896..12660)
                     /locus_tag="RPM73_000957"
     CDS             complement(11896..12660)
                     /locus_tag="RPM73_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6519343.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12746..13624)
                     /gene="araC"
                     /locus_tag="RPM73_000958"
     CDS             complement(12746..13624)
                     /gene="araC"
                     /locus_tag="RPM73_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDX6519344.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13963..15663
                     /gene="araB"
                     /locus_tag="RPM73_000959"
     CDS             13963..15663
                     /gene="araB"
                     /locus_tag="RPM73_000959"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDX6519345.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15674..17176
                     /gene="araA"
                     /locus_tag="RPM73_000960"
     CDS             15674..17176
                     /gene="araA"
                     /locus_tag="RPM73_000960"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDX6519346.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17345..18040
                     /gene="araD"
                     /locus_tag="RPM73_000961"
     CDS             17345..18040
                     /gene="araD"
                     /locus_tag="RPM73_000961"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDX6519347.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18115..20466
                     /gene="polB"
                     /locus_tag="RPM73_000962"
     CDS             18115..20466
                     /gene="polB"
                     /locus_tag="RPM73_000962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDX6519348.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20631..23537
                     /gene="rapA"
                     /locus_tag="RPM73_000963"
     CDS             20631..23537
                     /gene="rapA"
                     /locus_tag="RPM73_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDX6519349.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23549..24208
                     /gene="rluA"
                     /locus_tag="RPM73_000964"
     CDS             23549..24208
                     /gene="rluA"
                     /locus_tag="RPM73_000964"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDX6519350.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="RPM73_000965"
     CDS             complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="RPM73_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDX6519351.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25395..27749
                     /gene="lptD"
                     /locus_tag="RPM73_000966"
     CDS             25395..27749
                     /gene="lptD"
                     /locus_tag="RPM73_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDX6519352.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27802..29088
                     /gene="surA"
                     /locus_tag="RPM73_000967"
     CDS             27802..29088
                     /gene="surA"
                     /locus_tag="RPM73_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDX6519353.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29088..30077
                     /gene="pdxA"
                     /locus_tag="RPM73_000968"
     CDS             29088..30077
                     /gene="pdxA"
                     /locus_tag="RPM73_000968"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDX6519354.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30074..30895
                     /gene="rsmA"
                     /locus_tag="RPM73_000969"
     CDS             30074..30895
                     /gene="rsmA"
                     /locus_tag="RPM73_000969"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDX6519355.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSAEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30898..31275
                     /gene="apaG"
                     /locus_tag="RPM73_000970"
     CDS             30898..31275
                     /gene="apaG"
                     /locus_tag="RPM73_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDX6519356.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31282..32130
                     /gene="apaH"
                     /locus_tag="RPM73_000971"
     CDS             31282..32130
                     /gene="apaH"
                     /locus_tag="RPM73_000971"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDX6519357.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNVFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32155..32754
                     /locus_tag="RPM73_000972"
     CDS             32155..32754
                     /locus_tag="RPM73_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HDX6519358.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32790..33257
                     /locus_tag="RPM73_000973"
     CDS             32790..33257
                     /locus_tag="RPM73_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519359.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     SVAIDYGFFCSVSQQSIMNITRMAEDVWITESKTTQLIDILQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(33356..>33469)
                     /locus_tag="RPM73_000974"
     CDS             complement(33356..>33469)
                     /locus_tag="RPM73_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDX6519360.1"
                     /translation="LNLQATLQWNEVNEDAISALNEFAEETGCFSDEYRKF"
BASE COUNT         8101 a         9040 c         8875 g         7453 t
ORIGIN      
        1 aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca
       61 ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg
      121 atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca ccgcagcaca
      181 attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt gcgtcagggt
      241 tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt
      301 taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg
      361 gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc
      421 cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac
      481 catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca
      541 ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt
      601 tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac
      661 catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggagaaaga
      721 tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat
      781 tgccacttcg ccaatgcaca tcgccaccaa gctgcgcagc acgctggatg aagtgatcga
      841 acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg
      901 cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa
      961 tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt
     1021 cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc
     1081 cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc
     1141 cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc
     1201 gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc
     1261 cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc
     1321 gatcccggca aacaaagcca ttgttggcag cggcgcattc gctcactcct ccggtatcca
     1381 ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg
     1441 tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg
     1501 catggatgag atggggtata aagaaagtga atataattta gacaatttgt acgatgcttt
     1561 cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt
     1621 catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc
     1681 tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc
     1741 agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac
     1801 tgactataac gtcgaactgg tgaaatacag cctgactgct aaaggtcacg gtaaagatgc
     1861 tctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg gcgtcggcct
     1921 ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg
     1981 gcgcgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg aaaacaacaa
     2041 ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga cggtattggt
     2101 ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg
     2161 cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca cgggcagccg
     2221 ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta
     2281 ggcggcccga agtgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgctg
     2341 cctctgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg
     2401 ctggaagcat tctgtccgct gcgtgcagac attgccgcaa acggcttcga catcctgtgt
     2461 gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga
     2521 caatatgaaa aagcctttga taccgaggtg tatcaccgtt ttgagatcga acgtatcgcc
     2581 cgcatcgcgt ttgaatctgc tcgcaagcgt cgccacaaag tgacgtcgat cgataaagcc
     2641 aacgtgctgc aatcctctat tttatggcgg gagatcgtta acgagatcgc cacggaatac
     2701 ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat
     2761 ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag
     2821 tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa cgagcaaggt
     2881 tttggactgt atgaaccggc gggcggctcg gcaccagata tcgcaggcaa aaacatcgcc
     2941 aacccgattg cacaaatcct ttcgctggca ctgctgctgc gttacagcct ggatgccgat
     3001 gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg cattcgcacc
     3061 ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc
     3121 cgctatgtag cagaaggggt gtaatcatgg ctaagacgtt atacgaaaaa ttgttcgacg
     3181 ctcacgttgt gtacgaagcc gaaaacgaaa ccccactgtt atatatcgac cgccacctgg
     3241 tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt cgcccggtac
     3301 gtcagccggg caaaaccttc gctaccatgg atcacaacgt ctctacccag accaaagaca
     3361 ttaatgcctg cggtgaaatg gcgcgtatcc agatgcagga actgatcaaa aactgcaaag
     3421 aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg
     3481 ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg
     3541 ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg
     3601 tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt gaagtccagg
     3661 gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc ggtaaaaccg
     3721 gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc cgtgatttaa
     3781 gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca aaagccggtc
     3841 tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtctgcat gcgccgaaag
     3901 gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa
     3961 ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca
     4021 ccaatcccgg ccaggtgatt tccgtgaacg acaatattcc cgatccggct tcgtttgccg
     4081 atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggca
     4141 ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca
     4201 ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc
     4261 aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg
     4321 ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg
     4381 cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact
     4441 ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg
     4501 ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg
     4561 gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat
     4621 accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg
     4681 catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc
     4741 gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc
     4801 tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt taaagtggtg
     4861 attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg
     4921 gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg
     4981 atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc
     5041 tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag tattgggctt
     5101 accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc gtttatgaat
     5161 taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc
     5221 tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta cccagtagac
     5281 agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca gaattacccc
     5341 ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag gcattaaatc
     5401 ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg
     5461 cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc
     5521 tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc
     5581 cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt
     5641 gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt acatggtgtt
     5701 gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgtcactat cctgccagcc
     5761 accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag acggaagcat
     5821 aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccgt
     5881 aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc atgccagaga
     5941 aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcata
     6001 ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac
     6061 accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg cattgccgat
     6121 agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt
     6181 tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat
     6241 ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag
     6301 cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat
     6361 acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa
     6421 aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg
     6481 atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc ttcaagcatg
     6541 gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa
     6601 tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac
     6661 ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct
     6721 gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac
     6781 acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg
     6841 ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa
     6901 cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac
     6961 ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg
     7021 cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc
     7081 gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa
     7141 acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa
     7201 ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt
     7261 ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc
     7321 tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat tgtgtcgccg
     7381 acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc gttactgctg
     7441 ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc
     7501 catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca actgaaaatt
     7561 caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa cgtctgggtt
     7621 ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc acagggcgag
     7681 gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc
     7741 acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact
     7801 aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg
     7861 ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc
     7921 ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag
     7981 cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg
     8041 catgaaggag agatcgagag cgatatctgg cttaatagcg ccaactttac gctgccgctg
     8101 gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg tattccgatc
     8161 gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc
     8221 cagcaactgg tcgccagtaa agcaatggtg ccgcttatcc accactggct gatcattcag
     8281 gggcaacgca gtatgcgcgg cctgcgcatg aataccctcg gctggttcga ttttaaatca
     8341 gcgtggtttg cgccaccgga tccatgattg ctggttgata acaaaatcac tacactaacg
     8401 ccgttctcaa cggggtgcca cgcagacgcg tgcgctgaga aaatacccgt cgaacctgat
     8461 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt
     8521 gaggtgcaaa gtgttaaaaa aatgtctgcc cctgctgttg ctgtgcacag cgcccgtttt
     8581 cgctaaaccc gttctgactg tttataccta cgattccttc gccgccaact gggggccagg
     8641 cccggtggta aaaaaagcct ttgaagccga ctgtaattgc gaactgaaac tggtggcgct
     8701 ggaagatggc gtttcgcttc tcaaccgtct gcgcatggaa ggcaaaaaca gtaaagccga
     8761 tgtggtgctg gggctggata acaacctgtt agacgccgcc agtaaaaccg gactatttgc
     8821 caaaagcggc gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
     8881 cgtaccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaatcc
     8941 gccgcaaagc ctgaaagagc ttgttgagag cgatcaaaac tggcgggtga tttatcagga
     9001 tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag tctatggcga
     9061 tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg
     9121 gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac
     9181 ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag
     9241 cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga
     9301 gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac
     9361 cggcaactgg atgtatccgg tggcaaacgt cacgctgcct gccggttttg aacaattgac
     9421 caaacccgca accacgctgg agttcacgcc agccgaagtg gcggcacaac gtcaggcatg
     9481 gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta
     9541 agcgccgcca cgctggtggt agcggttgcg ctggcggcgt ttctcgccct gtggtggaac
     9601 gcgccgcagg gtaactgggt ggcaatctgg caggacagct acctgtgtca tgtggtgcgc
     9661 ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc
     9721 gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca
     9781 atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt
     9841 cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac
     9901 ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta
     9961 ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg
    10021 atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg acaaatcccg
    10081 ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg
    10141 ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct gagttacgac
    10201 tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg ccttgggctg
    10261 gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct gctgcaaggc
    10321 tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aatagtgctg
    10381 gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt aaatcgccag
    10441 ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt
    10501 gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc
    10561 gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg
    10621 atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct caacaacacc
    10681 attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc
    10741 ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg
    10801 ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc
    10861 ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt
    10921 ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag
    10981 caaattggct cctatcgcag ccaggacggc gcggtcaccg cgttaattct gctactactc
    11041 tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga
    11101 tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga
    11161 gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgattgc
    11221 cggttttctg acgccagcca gcggttcgct gactatcgac agcgttgatc acaccacgac
    11281 gccgccgtca cgccgcccgg tatcgatgct gtttcaggag aacaacctgt tcagccacct
    11341 gacggtcgcg cagaacatcg ggctggggct taatccggga ttgaagctga acgcggcgca
    11401 gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa tggcgcggtt
    11461 accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg
    11521 cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca
    11581 ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt
    11641 gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg
    11701 gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga gtgcttcggc
    11761 actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc cttatccgac
    11821 ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat caggcaaccc
    11881 gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat acaccggcat
    11941 caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa ttaacggcgt
    12001 cagccacaac aaacggccgc gcgacaaata atgactcaaa cggtcagtcg ctttaccact
    12061 gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg ttgccagcag
    12121 caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag
    12181 aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat
    12241 gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg
    12301 gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt
    12361 tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa
    12421 acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga tccctgccag
    12481 ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag
    12541 caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac
    12601 caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat
    12661 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
    12721 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
    12781 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatactcgcg
    12841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
    12901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
    12961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag caaacatgct
    13021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
    13081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
    13141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
    13201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
    13261 ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
    13321 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
    13381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
    13441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
    13501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
    13561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
    13621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
    13681 attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta
    13741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
    13801 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
    13861 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
    13921 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc
    13981 ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag
    14041 atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg
    14101 aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa
    14161 accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac
    14221 acaaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg
    14281 gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa
    14341 gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctac
    14401 attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag
    14461 gacaccgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct
    14521 ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat
    14581 aagtcgctat ggcacgaaag ctggggtggc ttgccgccag ccagtttctt tgatgagctg
    14641 gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg gactgccgat
    14701 attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg
    14761 gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct
    14821 aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag
    14881 agcgttggcg agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag cgtggtacct
    14941 ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt
    15001 cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa
    15061 atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg
    15121 tctctggatc acctgccggt ggtgctcgac tggtttaacg gccgccgcac accgaacgct
    15181 aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg
    15241 ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc
    15301 gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag
    15361 gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac
    15421 cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac
    15481 atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc
    15541 gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc
    15601 gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt tgcgactcta
    15661 taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca ttggcagcca
    15721 gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa
    15781 tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc tgggcaccac
    15841 gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct
    15901 ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg atcaacggcc tgaccatgct
    15961 caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt gggacagtat
    16021 cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat
    16081 tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg ataaacaagc
    16141 ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata cccgtcatct
    16201 gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg gtgataaagt
    16261 tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca
    16321 ggtggtgaac tccatcagcg acggcgatgt taatgcgctg gtcgatgagt acgaaagctg
    16381 ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc
    16441 ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac
    16501 caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg tacagcgtct
    16561 gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg
    16621 catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac
    16681 ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg aagtctgccc
    16741 gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa
    16801 ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg tcgccagcct
    16861 gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacacc
    16921 gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc
    16981 aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct tcagccatgc
    17041 gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat
    17101 tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta
    17161 cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt
    17221 cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcattg
    17281 ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc tctcaggagt
    17341 caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc tgccaaaaca
    17401 caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc gcggcgtctt
    17461 tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata tggtcgtggt
    17521 tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg acacgccaac
    17581 tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata cgcactcgcg
    17641 ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaatcggca ccacccacgc
    17701 cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg
    17761 cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac agggtatcga
    17821 tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat ggggcaaaaa
    17881 tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata tggggatatt
    17941 ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg ataaacacta
    18001 tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata aaaccacagc
    18061 caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt cagcgtggcg
    18121 caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac agaagtctcc
    18181 ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca agagtccgtg
    18241 gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg tgaacaaggc
    18301 tttcgcctga caccgctggc gttaaaggat tttcatcgcc agccggtgta tggcctttac
    18361 tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc
    18421 gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca
    18481 ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg tctgaaaccg
    18541 catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac cacccgccac
    18601 ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta tatgctgggg
    18661 ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc cagccgcccg
    18721 ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt gatcatcggc
    18781 tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtctt
    18841 ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg ctttaaaaac
    18901 ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga ggcgctgaaa
    18961 tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga gctattaggc
    19021 gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc
    19081 gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc
    19141 ttccacaaaa ctgaaatcat gccattttta cttgaacgag caacggtgaa cggcctgccg
    19201 gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc gcgaatgcat
    19261 cgcgctggtt atgtcgcgcc taatctcggc gaagtgccac cgcacgccag ccctggcggc
    19321 tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga ctataaaagc
    19381 ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt ggaaggcatg
    19441 gcgcagcctg atccagagca cagtaccgaa ggttttctcg atgcctggtt ctcacgagaa
    19501 aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgc
    19561 cagggtaaca aaccgctgtc gcaggcgctg aaaatcatca tgaatgcctt ttatggcgtg
    19621 ctcggcacca ccgcctgccg cttcttcgat ccgcggctgg catcgtcgat caccatgcgt
    19681 ggtcatcaga tcatgcggca aaccaaagcg ttgattgaag cacagggcta cgacgttatc
    19741 tacggcgata ccgactcaac gtttgtctgg ctgaaaggcg cacattcgga agaagaagcg
    19801 gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga aacgctgcaa
    19861 aaacaacggc tgaccagcgc attagaactg gagtatgaaa cccatttctg ccgttttctg
    19921 atgccaacca ttcgcggagc cgataccggc agtaaaaagc gttatgccgg actgattcag
    19981 gagggcgaca agcagcggat ggtgtttaaa gggctggaaa ccgtgcgcac cgactggacg
    20041 ccgctggccc agcagtttca gcaggagcta tacctgcgta tcttccgtaa cgagccatat
    20101 caggaataca tccgcgaaac catcgacaaa ctgatggcgg gtgaactgga tgcgcggctg
    20161 gtttaccgta aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat
    20221 gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat
    20281 cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac
    20341 caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag
    20401 ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca acttgggcta
    20461 ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca
    20521 tccagtacca tagcgccctt tccattcctg gacctgaata acgccactac ctcataagca
    20581 cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta
    20641 cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg
    20701 cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg
    20761 cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc
    20821 atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata
    20881 tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata
    20941 gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg
    21001 accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt
    21061 acagcggcct gcgcggtcag cgtaccagcc tgattccgca tcagctcaac atcgctcatg
    21121 atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa
    21181 ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc
    21241 tgattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca
    21301 acctgcgctt tgcgctattt gatgatgagc gttatgccga agctcagcac gatgcttaca
    21361 acccgtttga caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac
    21421 agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc
    21481 acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag
    21541 agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctaggg atggaaagcc
    21601 acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg
    21661 aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca
    21721 aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag gatatcgagc
    21781 cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt caggagctgg
    21841 tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg
    21901 gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa actgccgcta ccgacgcagt
    21961 atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg
    22021 ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct
    22081 ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc
    22141 agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc
    22201 gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg
    22261 accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag
    22321 aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc
    22381 cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc
    22441 acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct
    22501 ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc actatttacg
    22561 atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg
    22621 acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacagg
    22681 gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag
    22741 aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg
    22801 atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg
    22861 atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg
    22921 atcgtgaagt ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga
    22981 tccgcaacgg tctggatctg attctttctg gcgataccgg tagcagcacg atttcactgt
    23041 taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag
    23101 cccaggctcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc
    23161 tgctggataa aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc
    23221 agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg
    23281 ttcacgctat ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg
    23341 atgcagcgcg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc
    23401 tgcgtgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc
    23461 agcaggtaat ggaaagcctg gatcaggcag gttggcgtct ggatgccctg cgtttgatcg
    23521 ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga
    23581 accctggttg gttatcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg
    23641 tttgttgtcg gtgccgggtc gtctggaaga gcacaaagac agcgtgatga cgcgcattca
    23701 gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat
    23761 tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga
    23821 gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag aaggtctggt
    23881 ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac
    23941 gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata acacggcaag
    24001 agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct
    24061 ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc
    24121 gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat
    24181 gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg taggcggata
    24241 aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt
    24301 cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata
    24361 tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc
    24421 tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat
    24481 ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgtcgg
    24541 gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa
    24601 ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa
    24661 ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa
    24721 cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac
    24781 actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa
    24841 cgcattttgc gccgcagtac gggaagcgcc atgaagattc attcggtcca tcaactggct
    24901 ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac
    24961 ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat
    25021 tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca
    25081 aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat
    25141 atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt
    25201 accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg
    25261 tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag
    25321 agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac
    25381 aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg
    25441 ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc
    25501 caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg
    25561 ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca
    25621 tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag
    25681 gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc
    25741 aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct
    25801 gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac
    25861 aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg
    25921 gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg
    25981 cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt
    26041 tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca
    26101 ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg
    26161 atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat
    26221 tccgctacct ctcccaagcg ggcgctggct tgatggaact ggactatctg ccttcagata
    26281 aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga
    26341 accactccgg ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg
    26401 atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa
    26461 cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc
    26521 agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg
    26581 ttaattacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc
    26641 actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca
    26701 tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa
    26761 cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg
    26821 aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg
    26881 atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt
    26941 acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg
    27001 actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta
    27061 accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta
    27121 atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa
    27181 catgggagaa tgacgacaaa acgggctcac tggtgtgggc aggcgatact tactggcgta
    27241 tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag
    27301 cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt
    27361 accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg
    27421 agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc
    27481 gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta
    27541 tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc
    27601 tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca
    27661 tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga
    27721 acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg
    27781 gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg
    27841 atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat
    27901 aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct
    27961 gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt
    28021 ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat
    28081 gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag
    28141 atgcgcagcc gtctggctta cgatggactg aactacaaca cctatcgtaa ccagatccgc
    28201 aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg
    28261 ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag
    28321 ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac
    28381 gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt
    28441 aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg
    28501 ggccgtattc aggagttacc cgggatcttc gcccaggcat taagcaccgc gaagaaaggc
    28561 gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg
    28621 cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa
    28681 ccgtcgccga tcatgactga cgaacaggca cgcgtgaaac tggaacagat tgctgctgat
    28741 atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc
    28801 tccgctaacc agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc
    28861 cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc
    28921 ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag
    28981 aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg
    29041 atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc
    29101 aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc
    29161 ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg
    29221 accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg
    29281 cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca
    29341 ctgcggggca gttagcgatt gaaaatggac attacgtggt ggagacgctg gcgcgagcgt
    29401 gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg
    29461 tcattaacga cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc
    29521 aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga
    29581 cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga
    29641 ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg
    29701 tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca
    29761 ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac gggccgctgc
    29821 ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt
    29881 accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta
    29941 cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg
    30001 gacgtggtaa agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga
    30061 ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg
    30121 gcaaaacttt ctcaacgatc agttcgtgat cgacagtatt gtttctgcca ttaacccgca
    30181 aaagggccag gcgatggtcg aaatcggccc cggtctggcg gcattgaccg aaccggtcgg
    30241 cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca
    30301 aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa
    30361 ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta
    30421 taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat
    30481 gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc
    30541 gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt
    30601 tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc
    30661 tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga
    30721 agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgccga
    30781 ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca
    30841 atattgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg
    30901 atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca
    30961 cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg
    31021 ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa
    31081 gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag
    31141 tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg
    31201 atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc
    31261 acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac
    31321 gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg
    31381 ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg ctatgtgaaa
    31441 tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta
    31501 tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg
    31561 gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag
    31621 aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctaca gaccgccaaa
    31681 gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact cctatccctt ctttctcgac
    31741 gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg
    31801 cgttttatca ccaacgtctt tacccgtatg cgtttttgct ttccaaacgg tcaactggat
    31861 atgtacagca aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt
    31921 cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc
    31981 aaaggtacgc cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta
    32041 acctgcctgc gctgggaaga taagcagtat tttgtccagc cgtcgaaccg gcataaggat
    32101 ttgggcgagg gagaggcggt cgcgtcttaa cgaattacca ttcagggagg aataatggct
    32161 gactattttg aaatacgtgc tgattacaat cagcacacca tcacggttta tcaggcttac
    32221 aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat ttggcgcatc tttttcattc
    32281 aaccgcatga cctggattaa accctctttt atgtggatga tggagcgtag caactgggga
    32341 ctgaaaaaag atcaacaaca cattctcgca atccgaatca aacgcacctt ctttgataca
    32401 ttgctggaac aagcggtgct aaccacgccg gaagcccatg tgtatccaca tgcaggcatt
    32461 tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt gggatcccga acgcagtata
    32521 aatggtaaaa aactggagca ccgatcatta cagcttggta tttcccgtaa cttgatcagt
    32581 caatttaacg aagacgccat tgttgccatt gatgatctga ctccgctggt gcgaaagtgt
    32641 cataaccttt tgataaacgg taaaacaaca caggcgaaat ccttcctgcc gccagaaaaa
    32701 atttaccctg ttagtgcagc tgcgagaaag gctttgggaa tgaaaaattc ctgagacaaa
    32761 tacgcgccta ttaagcggaa aatatctaag tgcgacaaat caacagcggg cttaatacca
    32821 gagaaattat tctggcaatg attgacgaag cagatgaacc cccatttgct gagcagcgga
    32881 cctggcatcg caggcgaatc gcttttctat gtgattgcat cagcgttgct atcgactacg
    32941 gcttcttctg ttctgtctct cagcagtcaa taatgaacat aaccaggatg gctgaagatg
    33001 tatggataac agaaagtaaa acgacccaac tcattgatat tttgcaacaa cttaagcagc
    33061 tttatccgcc agaaactatt ccctacgtcc ataagttacc cactaaggaa ttacttgaac
    33121 tgctactttc tagtcctgat gatcttcgaa taattgaaat tcctgacatc atcggtgatg
    33181 tttttgatga ccaaacctgg attcggttat tcaaaaaaca ctttgctgat attatccaac
    33241 tacgatgtga tgtgtaacaa tccaaaatat atcgccagac gatagaccaa aaagaaagta
    33301 tttagcagat actcaacatt ctgacttctg tacagaaata gcagagttta aaaacttaaa
    33361 acttccggta ctcatcagag aaacatcctg tttcttctgc aaattcattt aatgccgaga
    33421 tggcatcctc gtttacttca ttccactgta gagtggcttg aaggtttag
//
LOCUS       DAPPNJ010000026        33229 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000026 DAPPNJ010000000
VERSION     DAPPNJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.026"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            44..997
                     /locus_tag="RPM73_000975"
     CDS             44..997
                     /locus_tag="RPM73_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6519361.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(1072..1158)
                     /locus_tag="RPM73_000976"
     tRNA            complement(1072..1158)
                     /locus_tag="RPM73_000976"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1122..1124),aa:Leu,seq:gag)
     gene            complement(1173..1505)
                     /gene="secG"
                     /locus_tag="RPM73_000977"
     CDS             complement(1173..1505)
                     /gene="secG"
                     /locus_tag="RPM73_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDX6519362.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1733..3070)
                     /gene="glmM"
                     /locus_tag="RPM73_000978"
     CDS             complement(1733..3070)
                     /gene="glmM"
                     /locus_tag="RPM73_000978"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDX6519363.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(3063..3911)
                     /gene="folP"
                     /locus_tag="RPM73_000979"
     CDS             complement(3063..3911)
                     /gene="folP"
                     /locus_tag="RPM73_000979"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDX6519364.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(4001..5935)
                     /gene="ftsH"
                     /locus_tag="RPM73_000980"
     CDS             complement(4001..5935)
                     /gene="ftsH"
                     /locus_tag="RPM73_000980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDX6519365.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(6035..6664)
                     /gene="rlmE"
                     /locus_tag="RPM73_000981"
     CDS             complement(6035..6664)
                     /gene="rlmE"
                     /locus_tag="RPM73_000981"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDX6519366.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6790..7083
                     /gene="yhbY"
                     /locus_tag="RPM73_000982"
     CDS             6790..7083
                     /gene="yhbY"
                     /locus_tag="RPM73_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDX6519367.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7239..7715)
                     /gene="greA"
                     /locus_tag="RPM73_000983"
     CDS             complement(7239..7715)
                     /gene="greA"
                     /locus_tag="RPM73_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDX6519368.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7963..9396
                     /gene="dacB"
                     /locus_tag="RPM73_000984"
     CDS             7963..9396
                     /gene="dacB"
                     /locus_tag="RPM73_000984"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDX6519369.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9436..10608)
                     /gene="cgtA"
                     /locus_tag="RPM73_000985"
     CDS             complement(9436..10608)
                     /gene="cgtA"
                     /locus_tag="RPM73_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDX6519370.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10624..11589)
                     /locus_tag="RPM73_000986"
     CDS             complement(10624..11589)
                     /locus_tag="RPM73_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX6519371.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11716..11973)
                     /gene="rpmA"
                     /locus_tag="RPM73_000987"
     CDS             complement(11716..11973)
                     /gene="rpmA"
                     /locus_tag="RPM73_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDX6519372.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11994..12305)
                     /gene="rplU"
                     /locus_tag="RPM73_000988"
     CDS             complement(11994..12305)
                     /gene="rplU"
                     /locus_tag="RPM73_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDX6519373.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12564..13535
                     /gene="ispB"
                     /locus_tag="RPM73_000989"
     CDS             12564..13535
                     /gene="ispB"
                     /locus_tag="RPM73_000989"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDX6519374.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13763..14041
                     /gene="sfsB"
                     /locus_tag="RPM73_000990"
     CDS             13763..14041
                     /gene="sfsB"
                     /locus_tag="RPM73_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDX6519375.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14089..15348)
                     /gene="murA"
                     /locus_tag="RPM73_000991"
     CDS             complement(14089..15348)
                     /gene="murA"
                     /locus_tag="RPM73_000991"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDX6519376.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15403..15657)
                     /gene="ibaG"
                     /locus_tag="RPM73_000992"
     CDS             complement(15403..15657)
                     /gene="ibaG"
                     /locus_tag="RPM73_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDX6519377.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15817..16110)
                     /gene="mlaB"
                     /locus_tag="RPM73_000993"
     CDS             complement(15817..16110)
                     /gene="mlaB"
                     /locus_tag="RPM73_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDX6519378.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16110..16745)
                     /gene="mlaC"
                     /locus_tag="RPM73_000994"
     CDS             complement(16110..16745)
                     /gene="mlaC"
                     /locus_tag="RPM73_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDX6519379.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16764..17315)
                     /gene="mlaD"
                     /locus_tag="RPM73_000995"
     CDS             complement(16764..17315)
                     /gene="mlaD"
                     /locus_tag="RPM73_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDX6519380.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17320..18102)
                     /gene="mlaE"
                     /locus_tag="RPM73_000996"
     CDS             complement(17320..18102)
                     /gene="mlaE"
                     /locus_tag="RPM73_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDX6519381.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18110..18919)
                     /gene="mlaF"
                     /locus_tag="RPM73_000997"
     CDS             complement(18110..18919)
                     /gene="mlaF"
                     /locus_tag="RPM73_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDX6519382.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19129..20106
                     /locus_tag="RPM73_000998"
     CDS             19129..20106
                     /locus_tag="RPM73_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDX6519383.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20120..21106
                     /gene="kdsD"
                     /locus_tag="RPM73_000999"
     CDS             20120..21106
                     /gene="kdsD"
                     /locus_tag="RPM73_000999"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDX6519384.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21127..21693
                     /gene="kdsC"
                     /locus_tag="RPM73_001000"
     CDS             21127..21693
                     /gene="kdsC"
                     /locus_tag="RPM73_001000"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDX6519385.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21690..22265
                     /gene="lptC"
                     /locus_tag="RPM73_001001"
     CDS             21690..22265
                     /gene="lptC"
                     /locus_tag="RPM73_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDX6519386.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22234..22791
                     /gene="lptA"
                     /locus_tag="RPM73_001002"
     CDS             22234..22791
                     /gene="lptA"
                     /locus_tag="RPM73_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDX6519387.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22798..23523
                     /gene="lptB"
                     /locus_tag="RPM73_001003"
     CDS             22798..23523
                     /gene="lptB"
                     /locus_tag="RPM73_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDX6519388.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23571..25004
                     /gene="rpoN"
                     /locus_tag="RPM73_001004"
     CDS             23571..25004
                     /gene="rpoN"
                     /locus_tag="RPM73_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDX6519389.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25027..25314
                     /gene="hpf"
                     /locus_tag="RPM73_001005"
     CDS             25027..25314
                     /gene="hpf"
                     /locus_tag="RPM73_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDX6519390.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25432..25923
                     /gene="ptsN"
                     /locus_tag="RPM73_001006"
     CDS             25432..25923
                     /gene="ptsN"
                     /locus_tag="RPM73_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDX6519391.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25969..26823
                     /gene="rapZ"
                     /locus_tag="RPM73_001007"
     CDS             25969..26823
                     /gene="rapZ"
                     /locus_tag="RPM73_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDX6519392.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26820..27092
                     /gene="npr"
                     /locus_tag="RPM73_001008"
     CDS             26820..27092
                     /gene="npr"
                     /locus_tag="RPM73_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDX6519393.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27306..27938
                     /gene="yrbL"
                     /locus_tag="RPM73_001009"
     CDS             27306..27938
                     /gene="yrbL"
                     /locus_tag="RPM73_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDX6519394.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27935..28663)
                     /gene="mtgA"
                     /locus_tag="RPM73_001010"
     CDS             complement(27935..28663)
                     /gene="mtgA"
                     /locus_tag="RPM73_001010"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDX6519395.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28660..29313)
                     /gene="elbB"
                     /locus_tag="RPM73_001011"
     CDS             complement(28660..29313)
                     /gene="elbB"
                     /locus_tag="RPM73_001011"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDX6519396.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29543..31879)
                     /gene="arcB"
                     /locus_tag="RPM73_001012"
     CDS             complement(29543..31879)
                     /gene="arcB"
                     /locus_tag="RPM73_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDX6519397.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31975..32904)
                     /locus_tag="RPM73_001013"
     CDS             complement(31975..32904)
                     /locus_tag="RPM73_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDX6519398.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPYAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT         8312 a         8921 c         7993 g         8003 t
ORIGIN      
        1 tatttttact ttatgttttt atgctcagta aaaggaatga ccgatgaatg gtgaatattt
       61 tgtacgcctc gcattacata ctctgaaatg cactcaaaaa gatttagcaa gccacttagg
      121 tgtttcctca acacaaatta gcaagtggaa aaaaggggaa cacatgtccg ctgatatgga
      181 aaaaaagttc agaaatatta cacaaatagg agattattct cctcaactag ttgaatggac
      241 gggctcagtc gcgaatgctg agaaatggga tcgacttatc cattttcttg ctcgacgagc
      301 tatggacgat gctgaaacgg gttatataac acaacttctt actgacgaag atggtttttt
      361 ggttgaagaa acaattgatg tattaaagcg aataggtttt cctacacctc tttcctttcc
      421 tgaaggactt aatattgacg acgataatgc tgatgaagaa gaggcttttt gggagatatt
      481 agagagcaat gctcattgtt ctgtaattaa tgatatttat catgctctga atgatgttta
      541 tggtttctac atagcatatg ttgatgagtt gattcaggac gacgatttag atgtctattc
      601 aagcgaagcc atcaatattc agagttcatt gatatcactt gctgcatgca aaattgaaat
      661 tgatactcct gttgcgtcaa actttaaaga atttcgttat agagttaaaa aagactacga
      721 aaattggttg aatcaactca agatgatggc attcagggcg ggaattccgt taagagccga
      781 acttctggaa atggtttata acaccgctga ccaacttagc gttgcagcag aagccgaacg
      841 tttcgatttc aataaatctc gcattcatcc agatatatac atgaacgaga tccttactgg
      901 catgagaata attcaccaag tgcttccggt aataatgcaa aagttagaaa ttacagattt
      961 caaactagat gaaacagatt tatgcctggg taaatagtta tcaaattaag aatacttagc
     1021 ctggcttata aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga
     1081 ggacgggact tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac
     1141 caattccacc acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta
     1201 gccggagcag ctggttgagt ttgttcggtt ttcaccggtg cactcagatt ttcccattcg
     1261 ctacctttat tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat
     1321 aacgttgcca gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac
     1381 agcgtagcgg aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc
     1441 agcatgatca gaccaacaag gccaattgcc acaataagga aaactactaa aagagcttcg
     1501 tacataatca acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg
     1561 ggagttttta atctttccca ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc
     1621 gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt
     1681 cgctgagaaa aaaggcgaca atcgccgcct ttttagccag ttatctaacg ctttaaacgg
     1741 cttttactgc atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt
     1801 ccaccatcac gcgaattaac ggttcggtgc cggatttacg cagcaacacg cgtccacggt
     1861 tgcccagcgc agcttcaacc tctgcggtca cggctttaac tgactcatgc tcaagtggat
     1921 cgccgctacc tgcggtgtaa cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc
     1981 tgcaaaggtc gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag
     2041 caacgatgcc gtcaccggta gtagttttat ccagcaggat cacatgaccg gaattctctg
     2101 caccgatacg ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg
     2161 cgcgcgcaaa tggaattcct aactgtttca gcgccagttc aagccccatg ttgctcatca
     2221 atgtacccac agcgccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat
     2281 acatgatctg atcgccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc
     2341 catcgccgtc gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg
     2401 cgcgaacgtc ggtagccccc acttcggcat tgatgtttac accgtttggc tcacaaccga
     2461 tagcgataac gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac
     2521 cgtttgcaca atccaccaca atcttcagtt cactgaggct aagttcgttc gggaacgtgg
     2581 ctttgcaaaa ctcgatatag cgacccgcgg catcaacgat acggctggct ttacccagtt
     2641 ctgccgaatc aacgcagctg atctcctttt ccatttccgc ttcgatggcc tcttctaccg
     2701 catccggcag tttggtgccg tcgatagaga agaatttaat cccattatcg tagaacgggt
     2761 tatgcgatgc agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca
     2821 cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg
     2881 cttccagtgc tgactccagc atatagccag aaatacgcgt gtctttacca ataataatct
     2941 tacgggagcc gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat
     3001 caggtgtgat cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac
     3061 gattactcat agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct
     3121 tctacggttt ctttgacgtc atgaacacga atgatgtgcg cgccttgcat tgcggcaatg
     3181 accgcacagg ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca
     3241 atcatcgatt ttcgtgacat acccaccaac agcggcaggt tgaaatggtg aaattcagcc
     3301 aggcgcgcca gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg
     3361 agcaacaatt tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag
     3421 tagcgattca cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga
     3481 tttccctgca tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg
     3541 ccaggttcgg aaagggagcg gatatcatta ataatgtgag cgccaacttt cgctgactca
     3601 cggatgactt ctggtttgga tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt
     3661 gcctcaacca caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct
     3721 gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc cagcattgat catcagattc
     3781 gcatgtttca ccgcatctat cagcgagtta tgcgtgccgc catccgaaaa ggaatcaggc
     3841 gtgacgttga ggatccccat tacgtgagga tggctaaggt ccagtgaagt accctgggca
     3901 aagagtttca tgatgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc
     3961 cggggttttc ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct
     4021 ctgacatggt gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg
     4081 gactaccatt gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac
     4141 gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca
     4201 tgagagcatc tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac
     4261 gattatagtt acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat
     4321 cggacatatg tttcgctttc gctacgctac ggccgaggaa tacttcacct tcttcttccg
     4381 cgtacagcag tggacccaat ttctcagaga agccccactg ggtcaccatg ttacgtgcca
     4441 gattggtcgc aactttaata tcgttggacg caccggtaga aacatgttcc ggcccataga
     4501 tgatctcttc tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac
     4561 ggctggcgct gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc
     4621 gtgggataat cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg
     4681 catgacccgc ttcgtggtaa gccgttgatt ctttctgcgc ttccgtcatc accatggagc
     4741 gacgttccgc acccatcatg attttgtctt tcgctttctc gaactcaacc atcgacacaa
     4801 cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag
     4861 caccggagaa accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg
     4921 gtacgcgacg catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccga
     4981 ccacaacctg acggtcgaaa cggccaggac gcagcagggc cgggtcgaga acgtccggac
     5041 ggttagtcgc ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca
     5101 gcatctggtt cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct
     5161 ggcggcctac ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct
     5221 gttcgaacat gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag
     5281 aaccggagat agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg
     5341 ttttaccggt acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt
     5401 tctggaagcg gctcggctcg cgcagatact ctaccagttc agcaacttct tcttttgctt
     5461 cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg
     5521 ctttgctctt accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga
     5581 agaagatcca gacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca
     5641 gcaggcttgg ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat
     5701 ccagtaattt cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg
     5761 taacgttgat ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt
     5821 gtaggaaggt agagtaatcc accttacggc cattagactc gctgggccca aagctctgga
     5881 atactgacat cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac
     5941 tcaagggatt aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc
     6001 agcatctttc aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa
     6061 tatacacttc ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa
     6121 acagggagcg aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta
     6181 caaaactgcc acctggcgct aatacatcac gacacatttc taacgccagt tccaccagat
     6241 acatggcacg ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca
     6301 tgacaacctg gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat
     6361 cacgaaaatc gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc
     6421 aagcgatgat gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg
     6481 gagcagcacc aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta
     6541 tttcatcaag tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat
     6601 atttatcgct aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg
     6661 tcatttaact ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc
     6721 tatttggtga tatatgggag atggcggtag aatgacccgt tttcaatccc aacgtaagca
     6781 aaaaatacga tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg
     6841 ctcaagccag ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt
     6901 gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa
     6961 actaaaacct tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc
     7021 atcggtaaaa cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc
     7081 taagattatc ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag
     7141 gagagcaaaa tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt
     7201 ctttacaata catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt
     7261 acttcaaatt ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg
     7321 ccgatcaggc cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct
     7381 tcgtcatcgc caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt
     7441 acggtagcac caaaaataac gcgcccattg ttgggcattt tggtgacatc aatcacctgc
     7501 gcgttcgaca gcttggcttc gatgtcttta atacggcctt cgcagaaacc ctgctgttca
     7561 cgagctgcgt ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata
     7621 gcagcaatga tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt
     7681 tcagcgccgc gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg
     7741 atggggcaag tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg
     7801 accagaagca aaaaaatacc gacccgggta caagtcccag gtcagctaca attcacattt
     7861 tgatagtcat tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg
     7921 ccgtagtatg acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt
     7981 atcatcggat tgaccagctg tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac
     8041 gttactcaac tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg
     8101 gcccccgcta ttgattacca cagtcagcag atggcgctgc ccgccagtac gcagaaagtg
     8161 attactgcgc tggcggcgtt gattcaactc ggccccgatt ttcgtttcac cacgacgctt
     8221 gaaaccaaag gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt
     8281 gccgatccga cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct
     8341 ggcgtcaacc aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat
     8401 aaagcccccg gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgct
     8461 gccatagttg accgcaactg tttctccgtc tcgctctaca gtgccccaaa acctggtgat
     8521 atggctttta tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc
     8581 ctcccccgag gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg
     8641 aaccgcttta cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt
     8701 gccgtgcagg atggagccag ctatgccggg gcaattctga aagatgagtt aaaacaggca
     8761 ggtatcacct ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta
     8821 gttgccagta aacagtcggc cccgttgcac gatctgctta agattatgct gaaaaagtcg
     8881 gacaacatga tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtgcct
     8941 ggaacatggc gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc
     9001 gatattggaa acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc
     9061 cccgccacca tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt
     9121 atctccatgc tgccgcttgc gggctatgac ggctctttgc agtaccgtgc aggtctgcat
     9181 caggcgggcg tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac
     9241 ctggcgggat tcattaccac agcgagcggg caacgaatgg cgtttgtaca atatctttct
     9301 ggctatgcag tagaaccggc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa
     9361 agccgtttgt ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg
     9421 ccggggtttt gctgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc
     9481 ccagtcatca tcccagtcct catcatcctc ttcagcaatc tcttcaagct gctggcgatg
     9541 ataatcatcc cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac
     9601 cgggttttca atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccact
     9661 cgccgcagag atcagataat atttatcttc ccagcccagc gcttcagcaa tcgctttcgc
     9721 tttctcttca gcttctgcct tatccagcag atcgatctta ttgaacacta accaacgtgg
     9781 tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc
     9841 aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg
     9901 ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag
     9961 tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact
    10021 tggcaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg
    10081 aataaaggtc gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat
    10141 cagctccagc agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt
    10201 acggttaact gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac
    10261 cagcagacgc tgaccgtgtt tggtcatatc gcccatggtt tcaccagtac cctggtcgat
    10321 tacacgcgta cctaccggca ctttaattgt gacgtcttta ccgcgcttac cggtacagtc
    10381 gcggcttgcg ccattctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat
    10441 aagcgtgttc aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc
    10501 gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc
    10561 gccatcacct gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt
    10621 aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca
    10681 aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata
    10741 acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc
    10801 tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg
    10861 ccagcgagcc atcgcttccg ccagggcgcc atatcctacc aaggtattca gtccgcaaaa
    10921 aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg
    10981 gaagagcgca attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg
    11041 acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc
    11101 aaagataact ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt
    11161 aaaaaacatc accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat
    11221 tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat
    11281 cacctgcgaa gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc
    11341 aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg
    11401 taatggcggc aacctcttct tcaccgcaag aatggcacca aggccaatac tcgccatcaa
    11461 gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat
    11521 tggcaacgcc ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg
    11581 ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat
    11641 ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac
    11701 cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt
    11761 aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc
    11821 tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag
    11881 aacggattcg ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt
    11941 acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg
    12001 ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag
    12061 tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact
    12121 tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt
    12181 gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc
    12241 aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg
    12301 tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata
    12361 ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat
    12421 acacgaagaa aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac
    12481 aatcactcta tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg
    12541 gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag
    12601 atatggcggg tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc cagctgatca
    12661 atcagttagg ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg
    12721 tactggctgc acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga
    12781 tcgagtttat ccacacggcg actctgctgc acgacgacgt tgtggatgaa tcagatatgc
    12841 gcaggggtaa agctaccgcc aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg
    12901 attttattta tacccgcgcg ttccagatga tgaccagcct cggttcgctc aaagtgctgg
    12961 aagtcatgtc agaagccgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg
    13021 ttaacgatcc ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc
    13081 gtctgtttga ggctgccgcg cagtgttccg ggattctggc tggctgtacg ccggaagagg
    13141 agaaaggcct gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt
    13201 tactcgatta caatgccgat ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg
    13261 aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac
    13321 agatgatccg taccgccatc gaacagggta acggtcgcca tcttctggaa ccggttctgg
    13381 aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag
    13441 acaaagccat cgcagcatta caggtgctcc cggacacccc ttggcgagaa gcactcatcg
    13501 gcctcgcgca catcgctgtt caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg
    13561 cgcgggatga ttccagtaaa ttccataaaa acactaattc agcgctataa cacctgaaaa
    13621 aaagggcgat gaatctttat atattctgaa tattcacact ctttacagga actttttaga
    13681 gcaataggcc atcaggagta tagtgatgcc cgacagaaga agtgttctga atgaaagcga
    13741 ctaacttaag gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat
    13801 cattgcgggt ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt
    13861 gagttcctca acgctggcaa acgcattatc gcgcccatgg ccgaaaggag agatgattat
    13921 tgcgaaagcc ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca
    13981 gacccatgag tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg
    14041 agtggtctgg cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt
    14101 cacacgctca atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg
    14161 atcgatgtga taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac
    14221 caggcttgct gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac
    14281 accgtgacaa ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc
    14341 cggcacatgc ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc
    14401 caccaggttc aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc
    14461 ggtacgtacg ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc
    14521 gccgacttcg atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc
    14581 tggctgcgcg ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt
    14641 accggtttcg atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc
    14701 ttcgatgacg atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa
    14761 gttcgcggta tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc
    14821 cgccagggtt gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac
    14881 gatatgtgcg cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt
    14941 gatggtcgcg cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat
    15001 cgtacaaccg ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag
    15061 tgcccagata gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac
    15121 gtcgcgggca tcaatatgca cagaaccatt acgttctact ttcgcaccca gctggcttag
    15181 cagcttcatt gatgtatcga cgtctttcag tttcgggacg ttctggatct ctaccggttc
    15241 ttccgccagt agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt
    15301 gacttcgccc tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct
    15361 cagttaacaa ttcatatccg ctaccggcga atcgcccata gctcaaaagc cgttcagttt
    15421 gcgatcgcgc gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt
    15481 atccgcaata tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat
    15541 gccgtcaaac aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg
    15601 gacttcctgg agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg
    15661 atcttcaatc atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata
    15721 aataagcaaa aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg
    15781 gacaggcggt taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg
    15841 caaattatac aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt
    15901 gttgccctgc tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc
    15961 cacgcgggac acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat
    16021 ttcccaaagc ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc
    16081 cgtctgcatc cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt
    16141 tgagaaatcg atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt
    16201 ccccactcgt tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc
    16261 tgccagttgc ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg
    16321 ccattcgggt caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt
    16381 tctggcgcaa tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc
    16441 ttcaggtact cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg
    16501 taatactggc ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc
    16561 tgatcaacaa tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc
    16621 aggcgatcga acgttttctg cgccgcctcg tccatcagct tataaggatt ggtctggtct
    16681 gccgcggttg ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta
    16741 aacatgcgtc ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta
    16801 ttacctggcg cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg
    16861 taaaggaact gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta
    16921 tcgccatcct tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc
    16981 agatattgtt cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg
    17041 tggttataac gttgttcaat ttccagcgtt acgcgcggca gataggtttt cgggtccagc
    17101 gtaatatccg ccacccgacc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc
    17161 aggccgccaa tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac
    17221 gtcacgttcg ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag
    17281 atacccaccc aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc
    17341 ggtcagcaca aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt
    17401 tgcccggcta atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca
    17461 cgtcaccgtg atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca
    17521 gtcgacggca ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac
    17581 gccgaccaga gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa
    17641 tgaaataacc ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat
    17701 catctccata ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc
    17761 cgaaccagca cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag
    17821 cgataacgcc accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata
    17881 accttgcagc cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga
    17941 caggacgccg acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg
    18001 tttgccgacc agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt
    18061 aatcccttta tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg
    18121 gtaaaagatc agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta
    18181 tcccgtccag aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc
    18241 catgagcgac aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt
    18301 ccggcacatc gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa
    18361 tcagcttcac cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca
    18421 tgatgagatc cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac
    18481 cggaaagttc agaaggcatt agtttagccg ctccacgcag ccccacggcc tccagcttca
    18541 tcatcaccgt actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg
    18601 cgacgttgtc aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca
    18661 tccgtttgcg cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga
    18721 aaaggatctc accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt
    18781 taccgatgcc cgatggcccc atgatcgccg tgatcttccc tcgcggcacg gtcagggaaa
    18841 tattatcgaa gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat
    18901 tcgccacaga ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc
    18961 tcaatttaac cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc
    19021 tggcatttgt tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc
    19081 tgaaaatctt cagcggaccg gcgagtatac ctgaagaaag gacgttagat gcttttagct
    19141 acggcactgt taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt
    19201 gccgcgtcta ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg
    19261 gtcagtattg gtacatcgtt accagaaatc atcgtctcgc ttgccgcgtc tctgcacgaa
    19321 caacgcgatt tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc
    19381 ctcggtctgg ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa
    19441 ttacccttaa tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt
    19501 agtcgcagcg atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt
    19561 aaacttgcac gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca
    19621 gagctgccgc gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc
    19681 atcatgccag tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt
    19741 gccatcagcg agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa
    19801 ctggcaaccg caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc
    19861 attggcgcaa acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca
    19921 ggagagattg atccattggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt
    19981 atttttgcgt tgctgtgctg gcggcgctcc ccgcaaccgg gccgtggtgt aggggtatta
    20041 ttaactggcg gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt
    20101 gaataactgg aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca
    20161 agcaggtaaa gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat
    20221 caatcagaat ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt
    20281 catggggatg gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac
    20341 cggtacacct tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt
    20401 caccccacag gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc
    20461 cttaattcca gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga
    20521 gagcagcatg gcgcgcgccg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg
    20581 tccgttaggg ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct
    20641 cgctgtcgcg ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc
    20701 aggcggcgca ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga
    20761 tgagatcccg catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg
    20821 caaaaatctt ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac
    20881 cgacggtgat ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc
    20941 cgatgtgatg acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact
    21001 gaacttaatg cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact
    21061 cggtgtgtta catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa
    21121 acaacaatga gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac
    21181 gttatggcaa aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca
    21241 gatggcctga tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac
    21301 ggttatggca ttcgttgtgc gctcacctct gatattgaag tcgctatcat taccgggcga
    21361 aaggctaaac tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg
    21421 cagtcaaaca aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa
    21481 aatgtggctt atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta
    21541 agcgtcgccg tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc
    21601 attgctggcg gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc
    21661 aaactggatg aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg
    21721 tgctatcact ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg
    21781 cccaggtggt cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg
    21841 tctataaccc agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt
    21901 ccgatcaggc cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa
    21961 tcccgacatg gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt
    22021 tatatggaca cgttgaagtc aacgcacttg tgccagactc tcaacttcgc agaatcacga
    22081 cggataacgc gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt
    22141 tatacggaac aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga
    22201 acgccgagct gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc
    22261 cttaatcttg tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc
    22321 ggagacactg atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc
    22381 aacgtggtta cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc
    22441 gacaaagtgg tcgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac
    22501 ggtaaaccgg caacgttcta ccagatgcag gacaacggta aacccgttga aggtcacgct
    22561 tcccagatgc actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg
    22621 cagcaggtcg atagcaacat taagggcgat aagatcactt acctggtgaa agagcagaaa
    22681 atgcaggctt tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg
    22741 caggataaaa acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg
    22801 gcaacattaa ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac
    22861 gtcagcctga ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt
    22921 aagaccacca ctttctacat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt
    22981 attgatgatg acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc
    23041 tatctgccac aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg
    23101 gtactgcaaa ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg
    23161 atggaagagt ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt
    23221 gaacgtcgcc gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc
    23281 gacgaaccgt ttgccggggt tgacccgatc tcggttatcg acattaaacg catcattgag
    23341 cacctgcgcg acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg
    23401 gcggtttgtg aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct
    23461 acagaaatct tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc
    23521 tgatagggta gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag
    23581 gtttgcaact caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc
    23641 gtctgttgca gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta
    23701 atccgctgct tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca
    23761 gtgaaacgct ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa gagctgccgc
    23821 tcgatgccag ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact
    23881 acattgacga tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc
    23941 tgatgtggca ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta
    24001 tcgtcgatgc cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa
    24061 gtatgggcga tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac
    24121 ggtttgatcc ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct
    24181 cccaattcga taaaaccacg ccgtggctgg aagaggccag actgatcatt agcgatcatc
    24241 tcgatctgtt agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag
    24301 atgtgctgaa agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga
    24361 tccagactgg cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc
    24421 actggacggt agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg
    24481 cctcgatgtg caataacgcg cgcaacgatg gtgacagcca gtttatccgc agcaatctgc
    24541 aggatgccaa atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga
    24601 gtcgctgtat cgttgaacag cagcaagcct tctttgagca aggtgaagaa tatatgaaac
    24661 cgatggtact ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg
    24721 tgaccacgca aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt
    24781 ccagtcacgt caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg
    24841 tgaagaaatt aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct
    24901 ctttactgtc ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt
    24961 ctttatccat tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa
    25021 gacactatgc agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa
    25081 tttgttacag ctaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat
    25141 gttgttctga aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt
    25201 ggtgaaattc atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt
    25261 gataagctgg cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc
    25321 gggcaattag catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta
    25381 atgctccgag cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat
    25441 aatgatacaa ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc
    25501 cactgtcaga gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt
    25561 agtctgccgc ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc
    25621 ggtatcggca atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc
    25681 gttggcgttt tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg
    25741 gtggatttac tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact
    25801 ctgtcactgg tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc
    25861 cagagcgatg aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg
    25921 tagttattcg gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg
    25981 atcgtcagcg gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg
    26041 ggtttttact gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg
    26101 gccgatcgcg agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca
    26161 gaaatattcg aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc
    26221 ctggatgccg accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg
    26281 ctttccagca aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag
    26341 cctctgcgtt cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg
    26401 gctgaaatgc tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt
    26461 gagtctttcg gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc
    26521 ttcttgccga acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct
    26581 gtcgccgcgt tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc
    26641 tatcttgaac tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc
    26701 attggttgta ccggcgggaa gcaccgttcg gtgtatattg cagagcaact ggcagactac
    26761 ttccgctcgc gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca
    26821 tgaccgtcaa gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa
    26881 tgaagctgtt tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag
    26941 gcaccgaggc cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac
    27001 ggcagattga agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg
    27061 ccctctttaa ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct
    27121 tcaaactccc cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt
    27181 tgtacatttt gtgcgctttt cactttcaga agaaccttaa gaaaacctta agaggcattg
    27241 tttaggtttt gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag
    27301 atggcatgat tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt
    27361 atgcgcatcc ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg
    27421 gcgataaaga gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag
    27481 actggagtgg aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg
    27541 tctacgatgt gattgcagat tttgacggta aaccctccat tacgctgacc gaatttgctg
    27601 aacaatgccg ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc
    27661 gttatttgca ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc
    27721 accgcatcag tgagtcagag gttatcccgg tcgtctgtga caacattggt gaaagcacat
    27781 tgattccact ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga
    27841 aacgatttat cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg
    27901 agagcaaaac gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa
    27961 acggctcacc gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt
    28021 agcccgatgg cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg
    28081 cagcttccga ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt
    28141 cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct
    28201 ttttgctcca gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc
    28261 agctacgccc atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac
    28321 cgcgaatacg gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat
    28381 caaagcccca gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc
    28441 acggcgagat ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat
    28501 gcagccaggc gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac
    28561 taaacaacgc gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa
    28621 aacggcgaac gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac
    28681 gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata
    28741 tgctggggtg gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg
    28801 cacatgctcc gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt
    28861 caaacgcagc gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc
    28921 aagcggtttt ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt
    28981 gcattcgcta ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg
    29041 cacaatcaac gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg
    29101 cgttattcgt gccgcttcaa tcagcacatt gcgcgtttcc gtcatcgctt cgccagttaa
    29161 atggttgata acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct
    29221 gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc
    29281 gcatccgctc agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga
    29341 aacacattaa tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac
    29401 aaatcattta agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt
    29461 gtaactcatc ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc
    29521 gcgcaccccg gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca
    29581 cttctacgtc gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt
    29641 cccaggcagg aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc
    29701 ccactgaccc cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct
    29761 gcgccgtcag attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg
    29821 ctaacccgtc ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa
    29881 tatcgagcaa tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat
    29941 cctgggtatc ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac
    30001 tcagcacatc atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg
    30061 ttaaggccac cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga
    30121 tatccagccc ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg
    30181 gtttaaacat ctccagcgcc gccttaccgg tcatggcgac atcaacgctg ttacctaatt
    30241 tttccagcac agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat
    30301 tcagcgccgg taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg
    30361 acggtgcgtg gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa
    30421 tatcgccgcc catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg
    30481 tggcaggttt accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca
    30541 gctcatcctg cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt
    30601 catcgtagcg cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga
    30661 tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg
    30721 gcagcgtcgg ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat
    30781 tttccagatc ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc
    30841 gccgttccat cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg
    30901 aaacatggat ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa
    30961 tacggctcag gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga
    31021 taaacgtcgt tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg
    31081 taatgtcgcg accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca
    31141 ctttacggat ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg
    31201 tcagtgacac attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt
    31261 ccggtgagta aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca
    31321 gcagctccat cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca
    31381 ggtcgggtga agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt
    31441 gtgtctcttc gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg
    31501 cttccgcttt ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact
    31561 gaacgttgag gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca
    31621 gacgttgtcg tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg
    31681 gcgtaatcag caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat
    31741 gcagcaccat ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca
    31801 gcaacattga gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca
    31861 gcagacgaat ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa
    31921 ttctgacagt tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc
    31981 cgttggaggt atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata
    32041 gcgatccagc tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt
    32101 cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat
    32161 catttctccg gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc
    32221 ccaggctttc gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc
    32281 cacgccggtt tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg
    32341 caggccgaca attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt
    32401 acgctgataa caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc
    32461 ggtttgtagc cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag
    32521 atccagcacc gcatacggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt
    32581 ggcctggctc accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct
    32641 ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg
    32701 cgccagttgc tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt
    32761 acagaatgtg cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc
    32821 gtgtagcgtc agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat
    32881 attgactaat ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc
    32941 ctgccatttt tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc
    33001 aactgcataa tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc
    33061 ttatcttgtg tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa
    33121 acgtcattac caattaaggc agtataaaat gctggttttc tcgtcagttc aaggcaggat
    33181 aagggttaac acgcctttat gacagtcagg aattgactgt ttctctaac
//
LOCUS       DAPPNJ010000027        32751 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000027 DAPPNJ010000000
VERSION     DAPPNJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.027"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            15..575
                     /locus_tag="RPM73_001014"
     CDS             15..575
                     /locus_tag="RPM73_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6519399.1"
                     /translation="MVGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKIEVALG
                     HYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFP
                     NLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGIVNVDLIAY
                     YVSTVEASEITPGEADAVVNVTLDYR"
     gene            655..1350
                     /locus_tag="RPM73_001015"
     CDS             655..1350
                     /locus_tag="RPM73_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX6519400.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1380..3896
                     /locus_tag="RPM73_001016"
     CDS             1380..3896
                     /locus_tag="RPM73_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6519401.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQATELSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3907..4998
                     /locus_tag="RPM73_001017"
     CDS             3907..4998
                     /locus_tag="RPM73_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6519402.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5041..5901)
                     /gene="rsmI"
                     /locus_tag="RPM73_001018"
     CDS             complement(5041..5901)
                     /gene="rsmI"
                     /locus_tag="RPM73_001018"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDX6519403.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5965..8001
                     /gene="lpoA"
                     /locus_tag="RPM73_001019"
     CDS             5965..8001
                     /gene="lpoA"
                     /locus_tag="RPM73_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDX6519404.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7959..8354
                     /locus_tag="RPM73_001020"
     CDS             7959..8354
                     /locus_tag="RPM73_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDX6519405.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8374..8964
                     /gene="diaA"
                     /locus_tag="RPM73_001021"
     CDS             8374..8964
                     /gene="diaA"
                     /locus_tag="RPM73_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDX6519406.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8974..9549
                     /gene="yraP"
                     /locus_tag="RPM73_001022"
     CDS             8974..9549
                     /gene="yraP"
                     /locus_tag="RPM73_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDX6519407.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9663..10703)
                     /locus_tag="RPM73_001023"
     CDS             complement(9663..10703)
                     /locus_tag="RPM73_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX6519408.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10776..11411)
                     /locus_tag="RPM73_001024"
     CDS             complement(10776..11411)
                     /locus_tag="RPM73_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDX6519409.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11539..12057
                     /gene="yhbO"
                     /locus_tag="RPM73_001025"
     CDS             11539..12057
                     /gene="yhbO"
                     /locus_tag="RPM73_001025"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDX6519410.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12037..12480)
                     /locus_tag="RPM73_001026"
     CDS             complement(12037..12480)
                     /locus_tag="RPM73_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDX6519411.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12531..12833
                     /gene="yhbQ"
                     /locus_tag="RPM73_001027"
     CDS             12531..12833
                     /gene="yhbQ"
                     /locus_tag="RPM73_001027"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDX6519412.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(12820..13323)
                     /locus_tag="RPM73_001028"
     CDS             complement(12820..13323)
                     /locus_tag="RPM73_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX6519413.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13317..13841)
                     /locus_tag="RPM73_001029"
     CDS             complement(13317..13841)
                     /locus_tag="RPM73_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDX6519414.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14050..15045
                     /locus_tag="RPM73_001030"
     CDS             14050..15045
                     /locus_tag="RPM73_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDX6519415.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15054..15932
                     /locus_tag="RPM73_001031"
     CDS             15054..15932
                     /locus_tag="RPM73_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDX6519416.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            16138..17145
                     /locus_tag="RPM73_001032"
     CDS             16138..17145
                     /locus_tag="RPM73_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDX6519417.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17263..18507)
                     /gene="mtr"
                     /locus_tag="RPM73_001033"
     CDS             complement(17263..18507)
                     /gene="mtr"
                     /locus_tag="RPM73_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDX6519418.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18661..20550)
                     /gene="deaD"
                     /locus_tag="RPM73_001034"
     CDS             complement(18661..20550)
                     /gene="deaD"
                     /locus_tag="RPM73_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDX6519419.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20543..20623)
                     /gene="yrbN"
                     /locus_tag="RPM73_001035"
     CDS             complement(20543..20623)
                     /gene="yrbN"
                     /locus_tag="RPM73_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDX6519420.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20730..21614)
                     /gene="nlpI"
                     /locus_tag="RPM73_001036"
     CDS             complement(20730..21614)
                     /gene="nlpI"
                     /locus_tag="RPM73_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDX6519421.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21723..23858)
                     /gene="pnp"
                     /locus_tag="RPM73_001037"
     CDS             complement(21723..23858)
                     /gene="pnp"
                     /locus_tag="RPM73_001037"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDX6519422.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24105..24374)
                     /gene="rpsO"
                     /locus_tag="RPM73_001038"
     CDS             complement(24105..24374)
                     /gene="rpsO"
                     /locus_tag="RPM73_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDX6519423.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24523..25467)
                     /gene="truB"
                     /locus_tag="RPM73_001039"
     CDS             complement(24523..25467)
                     /gene="truB"
                     /locus_tag="RPM73_001039"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDX6519424.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25467..25868)
                     /gene="rbfA"
                     /locus_tag="RPM73_001040"
     CDS             complement(25467..25868)
                     /gene="rbfA"
                     /locus_tag="RPM73_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDX6519425.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26032..28704)
                     /gene="infB"
                     /locus_tag="RPM73_001041"
     CDS             complement(26032..28704)
                     /gene="infB"
                     /locus_tag="RPM73_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDX6519426.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28729..30216)
                     /gene="nusA"
                     /locus_tag="RPM73_001042"
     CDS             complement(28729..30216)
                     /gene="nusA"
                     /locus_tag="RPM73_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDX6519427.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30244..30696)
                     /gene="rimP"
                     /locus_tag="RPM73_001043"
     CDS             complement(30244..30696)
                     /gene="rimP"
                     /locus_tag="RPM73_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDX6519428.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30903..30979)
                     /locus_tag="RPM73_001044"
     tRNA            complement(30903..30979)
                     /locus_tag="RPM73_001044"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30943..30945),aa:Met,seq:cat)
     gene            31327..32670
                     /gene="argG"
                     /locus_tag="RPM73_001045"
     CDS             31327..32670
                     /gene="argG"
                     /locus_tag="RPM73_001045"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDX6519429.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
BASE COUNT         7995 a         8842 c         8159 g         7755 t
ORIGIN      
        1 ttacaaaaac agctattgtg ggcttacttg cgcttttcgc aggtaatgcc gctgcaaccg
       61 atggtgaaat agtttttgat ggcgagattt taaaatccgc ttgtgaaatc aatgactctg
      121 ataagaaaat tgaagttgct cttggtcact ataatgccga acagtttcgt tctgttggcg
      181 atcgtagccc aaaaattcca ttcactattc ctttagttaa ctgcccagtg actgggtggg
      241 agcacgataa cggcaacgtt gaagcgtctt tccgtttatg gctggaaacg cgggataatg
      301 gcaccgtccc taacttccct aatctggcaa aagtgggctc ttttgctggc acagcggcaa
      361 caggtgtggg tatccgtatt gacgatgcgg aaagcggaaa ccttatgcca ctgaatgcta
      421 tggggaatga taacacggtt tatcagatcc cagcggactc caatggtatt gtcaatgttg
      481 acctcatcgc ttactacgta tcaaccgttg aagcatcaga aatcacccca ggggaagcag
      541 acgctgtcgt taacgtaacg ctggattacc gttaatacgt tacggcgtta tctgaccttt
      601 cagataacgc ccttttcctt cctctttctc gttgtatcag gttgaaaaat aactatgtca
      661 aaacgaacat tcgcggtgat aataaccttg ttgtgtagct tctgtatcgg ccaggcgctt
      721 gcagggggta tcgttttaca gcgaacgcga gtgatctatg atgccagccg caaagaggct
      781 gcgttacctg tcgcaaacaa aggcgcagaa acgccttatt tactgcaatc atgggtagat
      841 aatatagatg gtacaagccg tgccccattt attataaccc caccgctatt tcgtcttgaa
      901 gctggcgatg actcatcact gcgaattatt aaaacagctg ataacctgcc tgaaaataaa
      961 gagtcgctgt tctacattaa tgttcgtgcc attccagcaa agaaaaaatc agatgatgtt
     1021 aatgctaacg agttgacgct ggtatttaaa acacggatca aaatgtttta tcgccccgca
     1081 cacctgaagg gacgggtaaa cgatgcgtgg acatcactgg aatttaaacg tagtgaccat
     1141 tcactcaata tatataaccc aactgaatat tacgtcgtat ttgccggact ggcagtcgat
     1201 aaaaccgatc tcacaagcaa aattgaatat atcgcgcccg gagaacataa acagttacca
     1261 cttcctgcat ctggcggaaa gaacgtgaaa tgggctgcaa tcaatgatta tggcggcagt
     1321 tccgggacag aaactcgtcc actgcaataa caaatataaa aaacacaggt catcagggaa
     1381 tgccacaacg acaccaccag ggacataaac gcacaccgaa acagttggcg ctcatcatca
     1441 aacgctgttt gccgatggtg ctcactggca gcggcatgct ttgcactacc gctaacgccg
     1501 aagagtatta tttcgacccc attatgctgg aaactacaaa aagtggtatg caagcaactg
     1561 aactgtcacg tttttcaaaa aaatacgcac aactaccagg aacttatcag gttgatatct
     1621 ggctgaataa aaagaaggtt tcacagaaaa aaattacatt taccgccaat gcagagcaac
     1681 ttctgcagcc acagtttacg gtagaacaac tacgtgagct gggtattaag gtggatgaaa
     1741 tcccggcgct ggctgaaaaa gatgacgata gcgtgatcaa ctcgcttgaa caaatcattc
     1801 ccggtacagc tgctgaattt gatttcaatc atcagcgact taatttgagc attccccaaa
     1861 ttgcactgta ccgtgatgca agaggttacg tctccccttc tcgttgggac gatggtatac
     1921 caacgctgtt taccaactac tcgtttacag gttctgataa ccgttaccgc cagggcaatc
     1981 gtagccaacg acagtacctg aatatgcaaa atggtgctaa ttttggcccc tggcgattac
     2041 gtaactattc tacgtggaca cgcaacgatc aggcgtcaag ctggaacact atcagtagtt
     2101 atttacaacg tgatatcaag gcgttgaagt ctcagttgct tctgggagaa agcgccacca
     2161 gcggcagtat tttttccagc tacaccttta ctggcgtgca actcgcttcc gacgataata
     2221 tgttgccaaa cagccagcgc ggatttgccc caacggtacg cggtatcgca aacagtagtg
     2281 caatcgtgac tatcaggcaa aatggttatg tgatctatca aagcaacgtg ccagcgggtg
     2341 cctttgaaat taacgatctc tacccctctt ccaacagcgg cgatttagaa gtcacgattg
     2401 aagaaagtga cggtacgcaa cgtcgcttta tccagcctta ttcttcatta cccatgatgc
     2461 agcgacctgg gcatctaaaa tatagcgcga ccgctggacg ctatcgcgct gatgcaaaca
     2521 gtgatagcaa ggaacccgaa tttgctgaag ccacggcaat atatggtttg aataatactt
     2581 ttacgctgta tggcggcctg ctcggttctg aagattatta tgcgctgggg atcggtatcg
     2641 gcggcacact tggcgcactg ggcgcgttgt cgatggatat caacagagct gacacccaat
     2701 tcgataaccg gcactctttt catggctatc aatggcgtac gcagtacatc aaagatatcc
     2761 cggaaaccaa caccaatatc gctgttagct actatcgcta taccaacgat ggctatttta
     2821 gttttgatga agccaatacc cgtaattggg actataacag tcgccaaaaa agtgaaattc
     2881 aattcaacat cagccagaca atatttgatg gggtaagtct gtatgcctcc ggttcgcagc
     2941 aagactattg gggcaataac gataaaaaca ggaatatctc agttggggtt tccggtcagc
     3001 aatggggaat tggttacagc ctgaattatc aatacagtcg ctacacggat caaaataatg
     3061 accgcgcact ctctttgaat ctcagtattc cgttagaacg ctggttaccg cgtagccggg
     3121 tttcctatca gatgaccagc cagaaagatc gcccaaccca acatgaaatg cgtcttgatg
     3181 gctcactgct ggatgatggt cgcctgagct atagcctgga acaaagtctg gatgacgata
     3241 acaaccataa cagtagcctg aacgccagtt accgttcacc ttatggcacc ttcagtgccg
     3301 gatacagcta cggtaatgac agcagccaat acaattacgg cgttaccggc ggcgtggtta
     3361 tccatcctca tggcgtgacg ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg
     3421 ctaatggggc ttctggcgtg aggatacaaa actatccggg gattgctact gatccctttg
     3481 gctatgcagt ggttccttat ctcacaactt accaggaaaa ccgtctctcg gtagatacta
     3541 cgcagctgcc cgataacgtc gatcttgagc aaacaacaca gtttgtggtg cccaacagag
     3601 gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta tcgcgtactt gttacagtca
     3661 gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc cagcaacgat gagacggggc
     3721 aacaaagtat cgtcgatgag ggcggcatac tatatctctc tgggatatcg agtaaatcac
     3781 aaagctggac tgtacgctgg ggaaatcagg cagatcaaca atgtcagttt gcttttagta
     3841 caccggattc agaaccaaca acctctgtat tacaaggcac ggcgcagtgc cattaaggat
     3901 aaaaaaatga aaagagcgcc tcttataaca ggacttttgt tgatatccac atcctgcgct
     3961 tatgcctcct caggagggtg tggagctgac agcactagcg gtgcgacaaa ttacagcagt
     4021 gtggttgatg atgttacagt gaaccagaca gataacgtga caggacggga gtttacctct
     4081 gccacgctaa gtagcactaa ctggcaatac gcctgtacct gctctgcggg taaggcagtt
     4141 aaacttgtct atatggtcag ccctgtactt accaccactg gacatcaagc aggatattac
     4201 aaactcaatg atagtctgga tattaaaacc acattacagg cgaatgacat tccaggactc
     4261 gtgactgacc agaccgtttc tgttaacacc cgattcacac agataaaaag caacaccgta
     4321 tattctgctg caacccaaac gggtgtttgc cagggtgaca cgtctcgtta tggacccgtt
     4381 aatattggtg caaataccac ctttaccctg tatgtcacca agccatttct cggctcgatg
     4441 accattccga aaacggatat tgccgtcatt aaaggcgcat gggtcgatgg aatgggaagc
     4501 ccgtctacag gtgacttcca tgatttagtc aagttatcga ttcagggaaa tctcaccgcc
     4561 ccacagtcgt gcaaaattaa tcagggcgat gttattaagg ttaattttgg attcatcaat
     4621 ggtcagaagt ttaccacccg caatgccatg ccagacggtt ttactccagt agactttgat
     4681 atcacttatg actgtggtga tacttcaaag attaaaaact cgttgcaaat gcgcatcgac
     4741 ggtacaactg gggtagtaga ccagtacaac ctggtcgcca gacgaagaag ttcagacaat
     4801 gcgcccgatg tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt
     4861 cagaacggta tccttcccgt tgatccttcc gggcatggca ccatcaacat gcgcgcctgg
     4921 ccagttaatc tggtcggtgg tgagctggaa acaggaaaat ttcagggcac cgccaccatt
     4981 accgtcatcg tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactc
     5041 ttacccctgc tgctccagcg catacttata cagcgcattt ttcttcacgc cgtgaatttc
     5101 tgccgccagc gccgccgctt ttttcaacgg cagttctgcc tgtagcagcg ccagcgtgcg
     5161 cagggcatcg gcgggtaagt cttcttcctg tgctttatga ccttcgataa tcagcaccat
     5221 ttcgcctttg cgacggtttt catcttcttt tacccacgcc agcagctcgc caacgggcgc
     5281 gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc
     5341 gcctaatacc gcaacgatat cttccaggct atctaacaga cggtgggtgg attcataaaa
     5401 aatcagcgtg cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt
     5461 ggcaggtaaa aagccttcgt aacagaaacg gtcagagggt aaacccgcgg cgcttaacgc
     5521 agtgatagca gcacacggcc cgggtagcgg caccacgcaa atccccgctt cacggcaggt
     5581 acgcaccaga tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc
     5641 aatgttctgc ccttcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg
     5701 gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg
     5761 acgagtatcc tcggcggcaa tcagatcaac ggcctgtaat acctctaacg cacgctgggt
     5821 gatatccgcc agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc
     5881 cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg
     5941 taaaaaaata tcactggata cattatggta ccctcaacat tttctcgttt gaaagccgcg
     6001 cgttgtctgc ctgttgttct ggcagccctg attttcgccg gttgtggcac ccatactccc
     6061 gatcagtcca ctgcttatat gcagggcacg gcgcaggctg attctgcctt ttatcttcag
     6121 cagatgcagc aaagctctga tgataccagg atcaactggc aattactcgc cattcgtgca
     6181 ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt ttaaccaact accgcaagaa
     6241 ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg tagagattaa actggcgcag
     6301 aaagattttg ctggcgcgca aaacttgctg gcgaaaatca cacctgccga tttagaacaa
     6361 aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca gccaggggcg tccttccatt
     6421 gatttactgc gcgcgttaat tgctcaggaa ccgctgctcg gcgcgaaaga aaaacagcag
     6481 aatattgatg ccacctggca ggcgctctcc tccatgactc aggaacaggc gaatacgctg
     6541 gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg atctgcagcg cgtctggttt
     6601 gataaccgta acgatcccga catgatgaaa gccgggatcg ccgactggca aaaacgttat
     6661 ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg ttaacgtaaa agcgtttaaa
     6721 ccagcctcga ccaacaaaat cgccctgctg ttgccgctga atggccaggc agcggtattt
     6781 ggtcgcacta ttcagcaagg ctttgaagcg gcgaaaaata tcggcactca gccagtggcg
     6841 gctcaggtag ctgccgcacc tgccgcagac gtagctgaac aacctcagcc gcaaaccgtg
     6901 gatggcgttg ccagcccggc acaagcctcg gttagcgatc tgaccggtga acagcctgcg
     6961 gcacagccag cgcctgtaag caccccggca gcaaccacca cagcggtaag cgcgccagcg
     7021 aatccatccg cagaactgaa aatctacgat acctcatcac aaccacttag ccagatctta
     7081 agccaggttc agcaggatgg cgcgagtatt gtggttggtc cgttgctgaa aaataacgtt
     7141 gaagagttgc ttaagagcaa caccccgctg aacgtgctgg cactgaacca gccggagaat
     7201 atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac cggaagacga agcgcgcgat
     7261 gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc tggtgctgat cccacgcagt
     7321 tcattgggcg atcgcgtagc caatgcgttt gcgcaggagt ggcagaaact gggcggcggc
     7381 accgttctgc aacaaaaatt tggttccacc agcgaattac gcgcgggtgt taacggcggt
     7441 tctggtatcg ctttaacggg taccccgatt actcccagag cgacaaccga ctccggcatg
     7501 acgaccaaca atccaacgct gcaaaccacg ccaaccgatg accagttcac caataatggc
     7561 ggtcgtgtcg atgcggtgta cattgtggca acgccgggtg aaatcgcttt tatcaaaccg
     7621 atgatcgcca tgcgtaacgg tagccagagc ggtgcaatgc tgtacgccag ctcccgcagc
     7681 gcacaaggca ccgcaggccc ggatttccgt ctggagatgg aaggtttgca gtacagcgaa
     7741 atcccgatgc tggcgggcgg taatctgccg ttaatgcagc aggcactcag cgcggtgaac
     7801 aacgattatt cgctggctcg catgtatgcg atgggcgttg atgcctggtc gttggcaaat
     7861 catttctcac aaatgcgcca ggttcagggt tttgaaatca acggtaatac cggaagtctg
     7921 acggctaacc cggattgcgt gattaacagg aagttatcat ggctacagta ccaacaaggt
     7981 caggtagtcc ccgccagtta accaccaaac agaccggcga tgcgtgggaa gcacaagcgc
     8041 gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc taacgtgaac gagcgtggcg
     8101 gcgagatcga tctgataatg cgtgaaggcc ggaccaccgt ttttgtcgag gtacgctatc
     8161 gccgctctgc gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa caacacaaat
     8221 tattacagac tgcccgcttg tggctcgcgc gtcataatgg gagttttgat actgtggatt
     8281 gccggttcga tgtggtagcc ttcaccggga atgaggttga gtggattaag gatgccttta
     8341 atgaccactc ataattaagg tttaaggatt agcgtgcaag aaagaattaa agcttgcttc
     8401 actgaaagca ttcaaactca aattgcggcg gcagaggcgc ttccggatgc catctcccgt
     8461 gcagccatga cgctggttca gtctctgctc aatggcaaca aaatcctctg ttgtggtaat
     8521 ggaacttccg ctgccaatgc acagcatttt gctgccagca tgatcaaccg tttcgaaacg
     8581 gagcggccca gcttacctgc cattgcacta aatactgata atgttgtctt aacggcgatt
     8641 gccaacgatc gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct gggtcatgcg
     8701 ggagatgtat tgttagccat ttccacccgt ggcaacagcc gcgatattgt aaaagcggta
     8761 gaagctgccg ttacgcgtga tatgactatt gtggcattga ccggctatga cggcggcgaa
     8821 cttgcaggtt tgttagggcc acaagatgtg gagatccgca ttccttcgca tcgtagtgct
     8881 cgcattcagg aaatgcatat gctgacggta aattgcctgt gcgatctgat cgataacacg
     8941 cttttccctc accaggatgt ttaaggagaa tacatgaagg cattatcgcc aatcgcagtc
     9001 cttatttccg cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg taccgctgct
     9061 gtgggtacca aagccgcaac tgacccacgc agtgtcggca cccaggtgga cgatggtacc
     9121 ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac agattaagaa agaagcccgt
     9181 attaatgtca cggcgtatca gggcaaagtg ctgctggttg ggcagtcacc aaacgctgaa
     9241 ctttcggctc gcgccaaaca gattgctatg ggcgtagacg gtgccaacga agtgtataac
     9301 gagattcgtc agggccagcc gattggtctg ggcgaagcgt ctaacgatac gtggatcacc
     9361 accaaagtgc gttcgcagct cttaaccagc gacctggtga aatcgtccaa cgtgaaagtg
     9421 accaccgaaa acggtgaagt gttcctgatg gggctggtga ctgaacgtga agcgaaagcg
     9481 gcggcagata ttgccagccg ggtgagcggc gtaaagcggg taaccacagc gtttacgttt
     9541 attaaatagc agccccttgt aatgcctgat gcgacgcttg ccgcgtctta tcaggcctac
     9601 aacgatgcaa accgtaggtc ggataaggcg tttacgccgc atccgacatg ttttccctca
     9661 aatcagaaca acagcgccag cccgccgaca atcacaccag acactgccac catcgcccct
     9721 gtcagccata aggctttcgc tgggaacgct ttgcgcagca taatcagtga cggcaaactc
     9781 accgccggga gcgtcatcaa caatgccagc gccggagcag ttcccatacc tgccagcatc
     9841 atcgtttgta caatcggaat ttctgctgcc gtgggaatga caaacaagca tcctgctacc
     9901 gccatcgcca tcacccacat caggctgtta tcgacagcac catcggcatg ggggaataac
     9961 cagacgcgag cggcacccaa caccagtact gcaaggatgt aaaccgggat cgtactccag
    10021 aaaagcgtcc atagcgccct gccccagcgg ctaaaaaaac cgccctgtgc ttccggtatg
    10081 tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca cccatttttg caccagcgtc
    10141 gcaatcagca acaccatcac cagcccggcc accagacgaa tcgccgcaaa accccagccg
    10201 aggacaaacc ccataaacac cagcgtcgcc gggtttaaca ccggattgcc catccagaat
    10261 gccagcgcac cgcccatcga cacctgttgg cgacgcattc ccgccgcgac cggagccgca
    10321 cagcaggtac acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt gccgcgaaag
    10381 cgcgattgcc caagcgtacg caacaaccag tcacgcggga tcagcacctg aatcaacgaa
    10441 ccgaggatca cccccagcac tgccgctttc cataccgcga ggaaatagat catcgcgtaa
    10501 tccaacgccg cctgccacgg attagcatcc gcctgcgcaa ggatagattt accgatactg
    10561 tgggtttcgg cagcagtaaa cgctttgccg tagtaaggtt gccatttcac ataccagaga
    10621 ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc actgaatggg cgttgccgcc
    10681 tgagatgaag actgaccagt catagcattc cccaggaata agttatgata attgagcgcg
    10741 tgaatattac gctcactatc aattcttggg aataattatt cagctctttt gcgtaattct
    10801 gaagagctta aaatcgtcac accttcatgc ccgggccgca tcgcctctgc cagcatgacc
    10861 cgcgccacgt ctctcgcatc aatggatttc cagttaccgg gtaataaacg gaacagcggc
    10921 gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat cgacgggcga
    10981 gcaatggtca atttcggcca gttctgggcg attaatgctt cttccatctc ccctttgacg
    11041 cggttataga aaaacggcga gtgggcattg gcacccatag cactgaccac cagcatatgc
    11101 tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta ccagcgtgta atcggcatga
    11161 ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc cgagacaaca aaacacaatg
    11221 tcgatgggat cggtgacctg cgccagcgcg tcactcagtt gcggatcatg gggattaaac
    11281 accccgggca tatcgcccaa cggacgtcgc gtcggcgcag caatggcgtt aactttcggt
    11341 tcgttaatca acatccgcag caggtgaccg cccaccagcc ccgttgcgcc tgtaatcagt
    11401 acctgactca tcttcgctcc tttacagaat tgtctgcctt gcgctccacg gctcatgcac
    11461 caggcttaat agaccatgag gtaattatcc ccgattgtgg gaaattcgcc tcatccaatg
    11521 caacaacgcg gaggaagcat gagtaagaaa attgccgttt taatcactga tgaatttgag
    11581 gattcagaat ttacttcacc cgcagacgag ttccgtaaag ccggacacga agtgattacc
    11641 attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag cgtgaccatc
    11701 gataaatcca tcgatgaagt gacgcctgcg gagtttgatg ccctgctgct accgggcggc
    11761 cattcaccgg attatctgcg tggggacaac cgttttgtca cctttacccg tgattttgtg
    11821 aatagtggca aaccggtgtt tgccatctgt cacggcccgc agttgctgat cagcgccgat
    11881 gtgattcggg ggcgcaagct gaccgcagtt aaaccgatca ttattgatgt taaaaatgcg
    11941 ggcgcggaat tttacgatca ggaagttgtg gttgataaag atcagctggt taccagccgg
    12001 acaccggacg atctgccagc gtttaaccgc gaggcgttac gcctgctcgg tgcctgagtc
    12061 gcgtagccaa atcatttttt taccaaagcc cagcgtgttg tcggtgaatt tgatttcatc
    12121 gagccggatt tcccataccg gggccgacag cattctggca accggaaagc gacggttgta
    12181 cgccttgcgt gcgaggtcgc tttcctcacc ttccagcctg cggatctcac ctttaaactg
    12241 cacaccgcga attaacgcta ctgttttcgg ctgaccgttt accgttccgg caactgccgc
    12301 ctgcggccca ctcatctgcg catgacgtgt tttttcttcc gtcaaaatgt agaaggcgac
    12361 tttctgtgca tcaaaaagat aaaaggcatt ggcgcaccag agttcccctt cctgctgcac
    12421 acaccaggtg acaacatgtt gttttgccag ccaacggctg atggcggtga gtgtttccat
    12481 tgctgttctc ttttatactg tgggcctgac cttaacatgc gatgcagacg atgacaccct
    12541 ggtttcttta cttgatccgt accgccgaca ataagcttta taccgggatc accacggatg
    12601 tcgaacgccg ctatcagcaa catcaaagcg gcaaaggggc gaaagcactg cgcgggaaag
    12661 gagaactgac gctggcgttt tctgcgccag tgggcgatcg ttcgctggcg ttacgggcag
    12721 aatatcgcgt taagcaactg accaaacggc aaaaagagcg tctggtagcg gaaggcgcag
    12781 ggtttgcaga gctgttgagc agcctgcaaa ccccaaagat taaaagcgat tgaaatgctc
    12841 gtgatactca accaggccgg taacgccatt cagcgcgtca tccgctaaac gatgtacctg
    12901 gaaggcgctt tcagtgcccg gccagcggca acgcagatcg tgatgcgccg ccagttcaaa
    12961 gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat agccgaactc
    13021 attaagcgaa tcgagtcctt catagaccag ttggcgtgcc agcccctgtc cacggtattt
    13081 ttcatcaacc gccaacggtg ccatgccgac ccattgcagg tcttcgccct gcacatcaac
    13141 cggactaaat gccacatagc caatgacctg accttcgtca tctgtcgcca ccagccccag
    13201 cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc gcttccgcat cactttcgaa
    13261 tgagcgacgt agcagggcat caatacccgg cgcatcaatg ggaatttcta ctcgaattag
    13321 catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc cgcctcaaca
    13381 aaatccgcca gttgcagcag catcatgcgt aaggctttcg gcatttgctc cagttcaatg
    13441 gcgtccatca ggtttttcac atacagcccc agctccgtat cgccttcaat caccagacga
    13501 cgctggaaga agagcgtatc cggatcttgt ttacgcgccg caatcatcag cagatcgctg
    13561 gcgtcggcac taaaactcac atcagcttgc gcgttctggc tgaccaccag tttgccattc
    13621 accaccgagg taaaccattg caggtcaata tcacgcacat gaatacttaa ccagcggcct
    13681 tcaagaaact ccagctcacc atcatccagc gcctgacgga attgccagct taagacctgc
    13741 tcaagaacct ggcgttttag cgcaaatggc gtcagtttta ccggtacact caacagagat
    13801 ggccccaaat gcacaatacg ggaacgcagt ttatccaaca cgagttttac tccctgtttc
    13861 aacaatcatc ctattttgcc atatcagaaa aataacatag cggtataaat caacaattcc
    13921 atatgaaatt gctgctacca ccaatacaac tttaactgcc ttaaatcaaa aattgtcgca
    13981 gcaaggttaa ctaaaatccc agttcgttaa catttttgcg ttttgatagc gcaaccttca
    14041 ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa ggcggccatc
    14101 gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg ataccaatgc ccgtcacttc
    14161 gccggcctta actttaccga gaaaaaattg caggaagcgg tgagttttgt ccatcaacat
    14221 cgccgcaaac ttcacatcgc gattaacact tttgcgcatc cggacggtta cgcccgttgg
    14281 cagcgcgccg tggatatggc ggcgcagctg ggtgccgacg cgctgatcct cgccgacctc
    14341 gccatgctgg aatacgccgc cgagcgttat ccgcatattg agcgccacgt atcggtgcag
    14401 gcttcggcga ccaatgaaga ggcgattaac ttttatcatc gccattttga cgttgcccgc
    14461 gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac aactggcacg ggtcacacct
    14521 gtaccactgg aagtctttgc tttcggcagc ctgtgcatta tgtcggaagg tcgttgctat
    14581 ctgtcgtcgt atctgacggg tgagtcgccc aacaccgtag gcgcgtgttc tccggcccgt
    14641 ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc gcctgaacga agtgctgatc
    14701 gaccgttatc aggacggcga aaacgcaggt tatccgacgc tatgtaaagg gcgttatctg
    14761 gtggacggcg agcgctatca cgcgctggaa gaaccaacca gtctcaatac cctggaactg
    14821 ctgccggagt taatggcggc gaatattgct tcggtgaaaa ttgaaggccg ccagcgtagc
    14881 ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg ctatcgaccg ttgtaaggcc
    14941 gatccgcaaa acttcatacc gcaaagcgcg tggatggaga cgctcgggtc gatgtccgaa
    15001 ggcacgcaga ccacccttgg cgcgtatcac cgtaaatggc agtgagaaga gcaatgaaat
    15061 attccttagg gccagtgctg tggtactggc caaaagagac gctggaagat ttttatcagc
    15121 aggccgccac cagcagcgcc gacgtgattt atcttggtga agcggtatgc agcaagcgcc
    15181 gggcaaccaa agttggcgac tggctggaga tggcaaaatc gctcgccgga agtggcaagc
    15241 agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc gaactgaaac
    15301 gctatgttga gaacggtgag tttctgattg aagccagcga tctcggcgtg gtgaatatgt
    15361 gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct gaactgctac aacgcggtga
    15421 cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg gagctttccc
    15481 gcgactggct ggtgaatctg cttaatcagt gcgatgagct gggcattcgc aaccagtttg
    15541 aagtggaagt cctgagctac ggtcatctac cgctggccta ctccgcccgc tgctttaccg
    15601 cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ctgcattaag tatccgaacg
    15661 ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc attcagacca
    15721 tgagcggcta cgtttacaac ctcggtaacg agctggcatc catgcagggg ctggttgatg
    15781 tggtccgcct gtcaccgcag ggtactgaca ctttcgcgat gctcgacgcc ttccgcgcta
    15841 atgaaaatgg cgctgcgcca ctattgttga cggcaaacag cgactgtaac ggctactggc
    15901 ggcggctggc aggactggag ctgcaagcct aagtaaatat gctcgtcata cttcaagttg
    15961 catgtgctgc atctgcattc gttcacccca gtcacttact tatgtaagct cctggggatt
    16021 cactctcttg tcgccttcct gcaactcgaa ttatttagag cacagctcac tttgttaaca
    16081 actttaacta ctctttaatg cagtattaaa gattaatcgg taacaaagtg agctgttatg
    16141 actgataaaa ccattgcgtt ttcgctactc gatctggccc ccattcccga aggttcttca
    16201 gcgcgagaag cattctccca ctctctcgat ctcgcccgtc tggctgaaaa gcgcggctat
    16261 catcgctact ggctggcaga acaccacaat atgactggca ttgccagtgc tgccacgtcg
    16321 gtattgattg gctatctggc ggcgaatacc accacgctgc atctggggtc tggcggcgtg
    16381 atgttgccta accactcacc gttggtcatt gccgaacagt tcggcacgct caatacactc
    16441 tatccggggc gaatcgattt ggggctgggt cgtgcgccag gtagtgacca acggacaatg
    16501 atggcgctgc gtcgccatat gagcggcgat attgataatt tcccccgcga tgtcgcggag
    16561 ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt accaggctat
    16621 ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagcgcgca actggcggcg
    16681 cagcttggtc tgccgttcgc gtttgcctca cacttcgcgc cggatatgtt gttccaggcg
    16741 ctgcatcttt atcgcagcaa cttcaaaccg tcggcacgac tggaaaaacc atacgcgatg
    16801 gtgtgcatca atattatcgc cgccgatagc aaccgcgacg ctgagtttct gtttacctca
    16861 atgcagcaag cctttgtgaa gttgcgccgt ggcgaaaccg ggcaactgcc gccaccgatt
    16921 caaaatatgg atcagttctg gtcgccgtcc gaacagtatg gtgtgcagca ggcgctgagt
    16981 atgtcgttgg taggtgataa agcgaaagtg cgtcatggct tgcagtcgat cctgcgcgaa
    17041 accgacgccg atgagattat ggtcaacggg cagattttcg accaccaggc gcggctgcat
    17101 tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt cttaacgcgg
    17161 gaagccttat ccgagctggc aacgctgtcc tacgtagacc tgataagcga agcgcatcag
    17221 gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat acaccggcag
    17281 taaattaaag ctcgataaaa tatggaccag tgcgttgcca acgccaaaca ccagaatcag
    17341 cgtaatcatc ggcttgccgc cccagacgcg gaatttcggg ctgccaaagc gtttacgcga
    17401 tgcgcgggct aacagcgccg gaacaattgc cgcccagatg gtagctgcta aaccagcata
    17461 accaatggcg tacaggaatc cgttcgggaa cagtagacca cccacaacag gcggggcaaa
    17521 ggtcagcaat gccgttttca agcgccccag ggctgagtca tcgaaaccaa acagatctgc
    17581 cagatagtca aacaaaccca gcgttacgcc aaggaacgaa ctcgctaccg caaagtttga
    17641 gaacacgacc agcagaaggt ccaggctacg gctgttcagc acgccgctta atgcctgtac
    17701 cagcacatca atattaccgc ccttctctgc aataccgata aactccggac gcgggatgtt
    17761 acccatcgtc gccagcaacc agatggtata cagcgccagt gccatcagcg taccgtacac
    17821 caggcatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc ttggcacgtt
    17881 accgtgataa ccaaacgacg ccaggcagaa cggcaatgtc atcagcagat acggcgcata
    17941 agatgcattg ctttctgcaa cgttgaacaa tgtcgcaggc tgcacatgcc ccaacaggct
    18001 gccaaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgccg tcatgcggct
    18061 gacggcctta gtactcaacc acaccacaaa cgctaccagc agtgcaaaac caaaacccgc
    18121 cgcccgtgcc gggacgttta gcgacatctc tgcgaaggtg tgatgcagaa ttgaaccact
    18181 ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa tgccgttgac
    18241 cacgttccag cctttgccca gcaaatcttt ggtgatagtg tcaaaactcg aaccgattcg
    18301 gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga accaggtgaa
    18361 gatcagcgcc gccattgacc agaagaacca cgccccggac atgaccactg gcagagaaaa
    18421 catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca gcgacggtga
    18481 cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa tagtgcgact
    18541 gcgttgttat gtattgcact gtaccagtac acgagtacaa aagacagaaa aaaagccccg
    18601 atggttcaaa tcggggctgt atatattatt ttacagactg tgttcgctgt tcagcgatga
    18661 ttacgcatcg ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg gtgcgcggcc
    18721 ttcacgacgt tcgccgctaa aacgacgacc atcaccacga ccaccttcac ggcgttcacc
    18781 gctgaagtta cgaccgcctt cacgacgctc gccaccgaaa ccacgaccac cgccacgacg
    18841 ctcaccgcca gtatgcggct gcgcatcgcc cagcaactgc atattcatcg gcttgttcag
    18901 aatgcgagta cgcgtaaagt gttgcagtac ttcacccggc atacctttcg gcagttcgat
    18961 agtggagtga gaagcaaaca gcttaatgtt accgatgtaa cggctgctga tgtcgccttc
    19021 gttagcaatc gcgccaacga tatgacgaac ttcaacacca tcatcgcggc ccacttcaat
    19081 gcggtaaagc tgcatgtcac caacatcacg acgttcacga cgcggacgat cttcacggtc
    19141 gccacgtggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt cacggaattc
    19201 acgtttcgga cgcatcggcg catctggcgg aacaatcaga gtacgttcac cctgtgccat
    19261 tttcagcagt gccgcagcca gagtttcgag atcctgctct tcaccttctg cagtcggctg
    19321 aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct gctgctgtac
    19381 tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg gcagttctac
    19441 ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac gctcgcggtt
    19501 ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac cgatacggtg
    19561 aacgtaagac tcagaatcca tcgggatatc gtagttaact accaggctga tacgctcaac
    19621 gtccaggcca cgggctgcaa cgtcggtcgc aatcaggatg tccagacgac catctttcag
    19681 gcgttccagt gtctgttcac gaagcgcctg gttcatgtca ccgttcagcg cggcgctgtt
    19741 gtagccgtta cgctcaagag cttcagccac ttccagagtc gcgtttttgg tacgaacgaa
    19801 gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt cgtttttgcg
    19861 cataccccag acagtccagt agctctggct gatgtcagga cgggtagtca cgctggactg
    19921 aatgcgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa tcgcttccgg
    19981 catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca taatggtttc
    20041 aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa ccagaccgct
    20101 cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc ccggagtacc
    20161 gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac gctggccgcc
    20221 gtacagagca accacattta cgccgcgcat gtgtttagag aaatccgtca ttgcttcagc
    20281 aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg ctttcagctc
    20341 aggatcaaga ttctgcaaca gaggtaaaga gaatgctgca gtttttccgc tccccgtctg
    20401 ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg cctgaattgg
    20461 agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct tcaggcccag
    20521 atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa ttaatggcgg
    20581 ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag tgtgaaccgg
    20641 ctcaaagtag gtgtattaac gaacaacaac gccctcaccc gttaaggtga tggcaatcaa
    20701 aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc caggtcatct
    20761 tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac aaagttatga
    20821 acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccaaatc ccccagactt
    20881 aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag cgaggtgtta
    20941 tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt gcccaggtag
    21001 aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg ctgtttcaac
    21061 acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca cagactacgg
    21121 aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc taacttgtca
    21181 cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg cgtagttgta agttggatca
    21241 agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc ctgcgttaaa
    21301 tatatgccta agtaattgaa tacttcaggc atatccggtc ggattgccag cgcttgcgaa
    21361 aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc gcgctcatat
    21421 aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg ttccatacgt
    21481 gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac ttcactttta
    21541 cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa acaccagcgc
    21601 aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc tgttcccggt
    21661 tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg caaatggcaa
    21721 ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg agactgctca
    21781 gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag aactttcacc
    21841 ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt gtcagcgatt
    21901 tgagagatgt gtaccagacc ttctttaccg ccgccgatgg caacaaatgc gccaaagtca
    21961 acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc agtgatctct
    22021 tcgatacgac gaatagcgtg tttcgctttc tcgccgtcgg tcgctgcgat cttcacagta
    22081 ccgtcatctt cgatttcgat ggtggtgcca gtttcttcgg tcagggcacg gataacagaa
    22141 ccgcctttac cgataacgtc tttgatcttg tccgggttga tcttgatggt atggatacgc
    22201 ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat tacgcccagg
    22261 atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc tttggtgata
    22321 ccttcaattt tgatatccat ctgcaatgca gagataccgt cgcgggaacc cgcaactttg
    22381 aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac aacgtagttg
    22441 tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat cggcacacct
    22501 gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga accgttggat
    22561 tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc cggcatgact
    22621 gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt cacgacgctt cggagaaccg
    22681 accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag gaaggtatcg
    22741 gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc cagcgttgcg
    22801 gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt acgcggcagc
    22861 acgccagtac gcacatccag accacggatc atatcttttt cacgaccgtc gatacgcggt
    22921 tcgcctgcca gtacgcggct acgaacaacg tttttctcga tagcgtgcag aatttcaccc
    22981 agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt ttcagatttg
    23041 atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc atcgctcagg
    23101 cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt cgtttaccgg ctccggctgc
    23161 cagtcccaac gtggtttacc ggcttctttc accagttcat tgatgttctg aataacaacc
    23221 tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct cagcagttca
    23281 gcttcagatt caaccatcag aacagcggct tcagtaccgg caacaaccag atccagtttg
    23341 ctctctttca gctcgtcctg agtcggattc agtacgtact ggtcattgat gtaacctacg
    23401 cgagcagcac caatcggacc attgaacgga ataccagaca gagacagtgc tgcggaagca
    23461 ccaatcatcg cgacgatatc cgggttaact tgcgggttaa cagaaaccac ggtggcgata
    23521 acctgaactt cgttgacgaa gccttccggg aacagcgggc gaatcgggcg gtcaatcaga
    23581 cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac gacggaagaa gctacccggg
    23641 atacgaccag cagcgtaggt acgctcctga tagttaacgg tcagtgggaa gaagtcctga
    23701 cctggtttgg cttttttctg gccaacaacg gtaacaaata ccgcggtgtc atccatgcta
    23761 accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt cacggtgtgt
    23821 tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt tctctttctt
    23881 agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct cgcgactaat
    23941 gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg aacctctgca
    24001 acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc cgtcagcttg
    24061 aaaaaagggg ccactcaggc ccccttttct gaaactcgca agaattagcg acgcagaccc
    24121 aggcgctcga tgagctgggt gtaacgtgct acgtctttac gtttcaggta gtcgagcagt
    24181 ttacgacgct gagaaaccat gcgcagcaga ccacgacggc tgtggtgatc ttttttgtgc
    24241 tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca gcagtgctac ctgaacttcg
    24301 gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa cgattttagc tgttgcttca
    24361 gtacttagag acattttaaa actccaaagt atatagaatg aaaggacgcc gatctctaat
    24421 tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat ttacgcgacg ttaagcggca
    24481 gtattctact cgtagcgacc tgttatcgca agacggttaa cattacgccg ggtattcaac
    24541 caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt tcgcccatac cgataaattt
    24601 gccgttctca ccttctgtaa cgcgaaccag tccttccagc ggcgcaccgg atgtacgaac
    24661 tgggttacca tttttgaagt aaacagaaga cgttaacgga agattcacca ccgggtagtc
    24721 cgaagctgga ctgtccattg gcatcagtaa tggatcaagt aactccgcag ccggaatatc
    24781 ctgctgttcg gcttgttcga ccagttcacg cagatgttcc agggtcacca tccgttcaac
    24841 cggatattta cttaccgcca gacgacgcag gtaaataaca tgcgcgccac aaccgagttt
    24901 ttcacccagg tcatcaatga tggtgcggat ataagtgcct tttgagcagt gaatttccag
    24961 ctccagctca ttgccttcgt ggcgaataaa cagcaattca taaacggtaa tcgggcgcgc
    25021 ttcacgcggt acttcaatgc cctgacgcgc atattcgtac agttttttac cctgatattt
    25081 cagtgctgaa tacatcgaag ggatctgttc gatatcgcca cggaaagtat ccagcgccgc
    25141 cgccagttgc tctgcgctaa aggttaccgg acgttcttca acgatttgtc cgtcggcatc
    25201 agaagtatcg gtacgctgtc caagacgcgc aatgacccga tagcgtttgt cggagtccag
    25261 cagatactgg gaaaacttcg tcgcttcccc gaggcaaatt ggcaacatgc cagttgccag
    25321 cgggtccagc gcaccggtat gcccggcacg gttggcgtta taaatccgtt tcactttttg
    25381 cagcgcatca ttgctggaca taccctgcgg tttatccagc aacaaaacgc cgttaatgtc
    25441 gcgaccgcga cgacgaggac gactcattag tcctccttgc tgtcgtccgg gttaacacga
    25501 cgttcttcgt catgtttgac cacgctggtc accaggtttg acatgcgcat cccttcaacc
    25561 agagagttgt cgtagaagaa ggtcagttcc ggcacgatac gcaggcgcat cgctttcccc
    25621 agcaggctgc ggatgaaacc agaagcttct tgcaacgctt tgatgcccgc tttaaccgcg
    25681 tcttcatctt tgtcgttgag gaacgtcaca tatactttgg catacgccag gtcgcgagac
    25741 atttcgacac cggaaacggt ggtcatcatg cccaggcgag gatctttaat ttcacgctgc
    25801 aggatgagag cgatctcttt ttgcatttcc tgcgctacgc gctgcgggcg accaaattct
    25861 ttcgccataa taaattctcc tgacaaaaaa ggggctgtta gccccttttt aaaattaatt
    25921 tcaggtggaa gggctgttca cgttggcctg ataagacgcg ccagcgtcgc atcaggcagt
    25981 ccatgcagga tgcggcgtaa acgccttatc ccgcatggaa ccctaaaaac cttaagcgat
    26041 ggtacgttgg atctcgatga tttcgaatac ttcgatcaca tcgccagtgc ggacgtcgtt
    26101 gtagttctta acgccgatac cacattccat accgttacgg acttcgttaa cgtcatcttt
    26161 gaagcggcgc agggactcca gctcgccttc gtagataacc acgttgtcac gcagaacgcg
    26221 gatcgggttg tgacgtttaa ccacaccttc ggtaaccata cagcctgcga tggcaccaaa
    26281 tttcggtgat ttgaacacgt cacgaacttc cgccagaccg ataatctgct gtttcagttc
    26341 cggagacagc ataccgctca tcgccgcttt cacttcgtca atcaggttat agatgacgga
    26401 gtagtaacgc agatccaggc tttccgcttc aatcacttta cgtgcagagg catcagcacg
    26461 tacgttgaag ccaaccagga tggcgttgga cgccgcagcc agggtggcgt cggtttcggt
    26521 gataccccct acgccagaac cgatgatctt cactttaact tcgtcagtag acagtttcag
    26581 caaggagtcg gagatcgctt ccacagaacc ctgtacgtct gccttcagga cgatattcac
    26641 ttcgtgaact tcgccttcgg tcatgttggc aaacatgttc tcgagtttag atttttgctg
    26701 acgcgccagt ttaacttcgc ggaatttacc ctgacgatag agtgcaactt cacgcgcttt
    26761 cttctcgtca cgtacaacgg taacttcatc acccgcagcc ggaacgccgg acaggccgag
    26821 gatttccacc ggaatggacg gacccgcttc cagcacttcc tgacccagtt cgttacgcat
    26881 cgcacgaaca cgaccgtatt cgaagccaca cagaacgata tcgcccttgt gcagagtacc
    26941 ttcacgtacc agaacggtag caaccggacc acgaccttta tcgaggaagg attcgataac
    27001 cgcaccgctc gccatacctt tacgtaccgc ttttagctcc agaacttccg cctgcagcag
    27061 gatagcgtcc agcagttcat cgataccggt acccgctttc gcagatacgt gtacgaactg
    27121 gctttcaccg ccccactctt ccggcaggat gccatactgg gagagttcgt tcttaacacg
    27181 atccggatcg gcttctggtt tatcgatctt gttcactgca accaccaccg gtacctgcgc
    27241 cgctttcgcg tgctggattg cttcgatggt ctgcggcatc acgccgtcgt cggcagcaac
    27301 aaccaggact acgatgtccg ttgcctgcgc accacgtgca cgcattgaag taaacgcggc
    27361 gtgccccggg gtgtccagga aggtgatcat gccgttttca gtttcaacgt ggtatgcacc
    27421 aatgtgctgg gtaatgccgc ccgcttcgcc agaggcaact ttcgttgaac gaatgtagtc
    27481 cagcagagag gttttaccgt ggtcaacgtg acccatgatg gtcacaaccg gcgcgcgcgg
    27541 ttcagccgca gcacccgtgt cacggtcgct cattaccgcc tcttccagct cgttttcacg
    27601 acgcaggata actttgtggc ccatctcttc agcaaccagc tgtgcggttt cctgatcgat
    27661 aacctggttg atggttgcca ttgcgcccag tttcatcatc gctttgatga cctgagagcc
    27721 tttaaccgcc atcttgttcg ccagttcgcc aacggtgatg gtttcgccga tcacaacgtc
    27781 acggttaacg gcctgagctg gcttctggaa gccttgctgc agggaagaac ctttacgttt
    27841 tccgccttta ccgccacgta ctgctgcgcg tgcttcttca cgatcagctt ttgattcagc
    27901 gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta cgaccacggc cacgaccgcc
    27961 ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca tgttgagaag tagtgacgtg
    28021 ataatcgctg gaatcttcag tcggttccgc gttatcagtc catttgtttt cttccgccat
    28081 acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg agtttacgac gcgcttcttc
    28141 ttcagcttta cgcttgagct ctgcagcttc ctgctcacgg cgggcttttt cagcctgggc
    28201 gtttttagtc atatcgtctt gttgattgct cactttgtct ttttccgcag cttcacgttt
    28261 cgcttgttca gcagcttcac gcttagcttg ttctgcggcc tcacgttcag ctttttgttg
    28321 cgcctcgcgt ttagccgatt cttctgcctc acgacgggct tgctcttccg cttcacgctg
    28381 cgcttgctct tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg
    28441 tttcttgcgg acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag
    28501 ggtgctgcgt gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag
    28561 gtggtcaatc aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat
    28621 acctgcatca gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag
    28681 cgttttaatc gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc
    28741 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa
    28801 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac
    28861 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc
    28921 gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc
    28981 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa
    29041 cagctctttc atcggcacat aggccagttc ttccagcgtc gagaagcctt cttctaccag
    29101 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg
    29161 cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc atcacgttga gttcccaacc
    29221 gctcagctgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt
    29281 accggcttca acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac
    29341 gtctgccggt gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat
    29401 atcgatacgc tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc
    29461 tacacaggca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg
    29521 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac
    29581 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc
    29641 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa
    29701 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcattgt tgcccagatc
    29761 cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg
    29821 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac
    29881 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac
    29941 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt
    30001 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct
    30061 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt
    30121 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga
    30181 tacggcttca actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga
    30241 ctattaaaag tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc
    30301 tttaccttcg acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca
    30361 tttacgacgg ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg
    30421 ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt
    30481 ataagcaacg gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc
    30541 agcacaatca tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt
    30601 gcgaccgcga ataaattcga tgccaaccag ttcaaaaccc agggcctcaa ctggcgcagt
    30661 aatcatctct gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa
    30721 gggcctaaag cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt
    30781 tatataactg aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt
    30841 caaatcccac tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa
    30901 agtggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac
    30961 caggctgctc caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg
    31021 caaatactgc tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg
    31081 atccacgcca cattttgtca acgtttattg ctaatcatgt gaatgaatat ccagttcact
    31141 ttcatttgtt gaatactttt gccttctcct gctccccctt aagcgcatta ttttacaaaa
    31201 aacacactaa actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt
    31261 gcataaaaat tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt
    31321 tattttatga cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt
    31381 tccggcggtc tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct
    31441 tatgcatata ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt
    31501 cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg
    31561 gccgaaggta ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc
    31621 tatttcaaca cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg
    31681 aaagaagatg gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa
    31741 cgtttctatc gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt
    31801 gatactgact ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc
    31861 tgcggtttcg actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt
    31921 ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac
    31981 ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca
    32041 gtacgctttg agcaaggtca tccggtagcg ctgaacggta aaacctttag cgacgacgta
    32101 gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag
    32161 attgaaaacc gtatcatcga agcgaaaagc cgtggtattt acgaggctcc ggggatggca
    32221 ctgctacata tcgcttatga acgtctgttg accggtattc acaacgaaga caccattgag
    32281 cagtatcatg cacatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac
    32341 tcccaggcgc tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt
    32401 gaagttaccc tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag
    32461 aacctgacct acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca
    32521 gatgatcgta ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa
    32581 ctttttggtt atgccaaaac tggcctgctc tcctcctctg ccacttcagg cgtgccgcag
    32641 gtggagaatc tggaaaacaa agttaagtaa tatcatagct ttcgtgctaa aggccgcaaa
    32701 tgcggccttt ttttacggct aagagaaagt gactgcgttt aggggccacc a
//
LOCUS       DAPPNJ010000028        32503 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000028 DAPPNJ010000000
VERSION     DAPPNJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.028"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            63..1352
                     /locus_tag="RPM73_001046"
     CDS             63..1352
                     /locus_tag="RPM73_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDX6519430.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1349..2815
                     /gene="hpaE"
                     /locus_tag="RPM73_001047"
     CDS             1349..2815
                     /gene="hpaE"
                     /locus_tag="RPM73_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDX6519431.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2817..3668
                     /gene="hpaD"
                     /locus_tag="RPM73_001048"
     CDS             2817..3668
                     /gene="hpaD"
                     /locus_tag="RPM73_001048"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDX6519432.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3678..4058
                     /locus_tag="RPM73_001049"
     CDS             3678..4058
                     /locus_tag="RPM73_001049"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDX6519433.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4126..4929
                     /gene="hpaH"
                     /locus_tag="RPM73_001050"
     CDS             4126..4929
                     /gene="hpaH"
                     /locus_tag="RPM73_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDX6519434.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4940..5728
                     /gene="hpaI"
                     /locus_tag="RPM73_001051"
     CDS             4940..5728
                     /gene="hpaI"
                     /locus_tag="RPM73_001051"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDX6519435.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5903..7279
                     /gene="hpaX"
                     /locus_tag="RPM73_001052"
     CDS             5903..7279
                     /gene="hpaX"
                     /locus_tag="RPM73_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDX6519436.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7289..8179
                     /gene="hpaA"
                     /locus_tag="RPM73_001053"
     CDS             7289..8179
                     /gene="hpaA"
                     /locus_tag="RPM73_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDX6519437.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8430..9992
                     /gene="hpaB"
                     /locus_tag="RPM73_001054"
     CDS             8430..9992
                     /gene="hpaB"
                     /locus_tag="RPM73_001054"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDX6519438.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10010..10522
                     /locus_tag="RPM73_001055"
     CDS             10010..10522
                     /locus_tag="RPM73_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDX6519439.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10915..13065
                     /gene="btsT"
                     /locus_tag="RPM73_001056"
     CDS             10915..13065
                     /gene="btsT"
                     /locus_tag="RPM73_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDX6519440.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13115..13318
                     /locus_tag="RPM73_001057"
     CDS             13115..13318
                     /locus_tag="RPM73_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDX6519441.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13329..14285
                     /gene="yjiA"
                     /locus_tag="RPM73_001058"
     CDS             13329..14285
                     /gene="yjiA"
                     /locus_tag="RPM73_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDX6519442.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14363..16903)
                     /locus_tag="RPM73_001059"
     CDS             complement(14363..16903)
                     /locus_tag="RPM73_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HDX6519443.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17255..17419)
                     /locus_tag="RPM73_001060"
     CDS             complement(17255..17419)
                     /locus_tag="RPM73_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDX6519444.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17596..19008
                     /locus_tag="RPM73_001061"
     CDS             17596..19008
                     /locus_tag="RPM73_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDX6519445.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19250..20194)
                     /locus_tag="RPM73_001062"
     CDS             complement(19250..20194)
                     /locus_tag="RPM73_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDX6519446.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20661..21893
                     /gene="mdtM"
                     /locus_tag="RPM73_001063"
     CDS             20661..21893
                     /gene="mdtM"
                     /locus_tag="RPM73_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDX6519447.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRSGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21934..23214
                     /locus_tag="RPM73_001064"
     CDS             21934..23214
                     /locus_tag="RPM73_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDX6519448.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRYAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23330..24481
                     /locus_tag="RPM73_001065"
     CDS             23330..24481
                     /locus_tag="RPM73_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDX6519449.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDTSRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24491..25258
                     /locus_tag="RPM73_001066"
     CDS             24491..25258
                     /locus_tag="RPM73_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519450.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25255..25512
                     /locus_tag="RPM73_001067"
     CDS             25255..25512
                     /locus_tag="RPM73_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDX6519451.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25577..26437
                     /locus_tag="RPM73_001068"
     CDS             25577..26437
                     /locus_tag="RPM73_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDX6519452.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26505..27683
                     /locus_tag="RPM73_001069"
     CDS             26505..27683
                     /locus_tag="RPM73_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519453.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27696..28249)
                     /gene="kptA"
                     /locus_tag="RPM73_001070"
                     /pseudo
     CDS             complement(27696..28249)
                     /gene="kptA"
                     /locus_tag="RPM73_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            28499..29182
                     /locus_tag="RPM73_001071"
     CDS             28499..29182
                     /locus_tag="RPM73_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519454.1"
                     /translation="MTQQGDAVVGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFIGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29179..29640
                     /locus_tag="RPM73_001072"
     CDS             29179..29640
                     /locus_tag="RPM73_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDX6519455.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGAITGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29653..30825
                     /gene="iadA"
                     /locus_tag="RPM73_001073"
     CDS             29653..30825
                     /gene="iadA"
                     /locus_tag="RPM73_001073"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDX6519456.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30890..31801
                     /gene="hypT"
                     /locus_tag="RPM73_001074"
                     /gene_synonym="qseD"
     CDS             30890..31801
                     /gene="hypT"
                     /locus_tag="RPM73_001074"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDX6519457.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31794..32186)
                     /gene="iraD"
                     /locus_tag="RPM73_001075"
     CDS             complement(31794..32186)
                     /gene="iraD"
                     /locus_tag="RPM73_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDX6519458.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32183..32266)
                     /gene="idlP"
                     /locus_tag="RPM73_001076"
     CDS             complement(32183..32266)
                     /gene="idlP"
                     /locus_tag="RPM73_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDX6519459.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         7479 a         8554 c         8520 g         7950 t
ORIGIN      
        1 tcgggaataa aagtaatcat taacatatta atgattaaaa tgatcgaatc cgaggagtgg
       61 taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt gatgcatggc
      121 aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc tggtttatta
      181 aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg
      241 tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag
      301 aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc ctgccggaag
      361 agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc cccattggcg
      421 aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc aacgggcgtc
      481 ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc
      541 tgagcgaatt tgccacgctg aatccaggcg acgccattct gctcggcacg ccacaggcgc
      601 gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg
      661 aaaatccggt agtggacgaa cgtgaagtga ccacgcgcaa gagcttccca acgcagccac
      721 acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc agcgaactgg
      781 aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg
      841 ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa gcggagctgg
      901 tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg
      961 cgggctacac cgtgtgtaac gactacgcca ttcgcgacta tctggaaaac tactaccgcc
     1021 ctaacctgcg ggtcaaaagc cgcgacggac tgacgccgat gctttcaacc atcgtgccga
     1081 aagaggcgat cccggacccg cataatctga cccttcgcac cttcgtcaac ggcgagttac
     1141 gccagcaagg caccaccgcc gatctgatct tcagcgtgcc cttcctgatc gcctacttaa
     1201 gcgaatttat gaccctgaat ccgggcgaca tgatcgccac cggcacacca aaaggcttat
     1261 ctgacgtggt gcctggcgat gaagtagtgg tggaagtaga aggcgtgggc cgcctggtga
     1321 accgaattgt gagtgaggaa acagcgaaat gaaaaaagta aatcattgga tcaacggcaa
     1381 aaatgttgca ggtaacgact acttccagac caccaatccg gcaacaggtg aagtgctggc
     1441 ggatgtggcc tctggcggtg aagcggagat caatcaggcg gtagcggcag cgaaagaggc
     1501 gttcccgaaa tgggccaatc tgccaatgaa agagcgtgcg cgcctgatgc gccgtctggg
     1561 cgatctgatc gaccagaacg tgccagagat cgccgcgatg gaaaccgcgg acaccggcct
     1621 gccgatccat cagaccaaaa atgtgttgat cccacgcgct tcccacaact ttgaattttt
     1681 cgcggaagtc tgccagcaga tgaacggcaa gacctatccg gttgacgaca agatgctcaa
     1741 ctacacgctg gtgcagccgg tgggcgtttg tgcgctggta tcgccgtgga acgtaccgtt
     1801 tatgaccgcc acatggaagg tcgcgccgtg tctggcgctg ggcaataccg cggtactgaa
     1861 aatgtcggaa ctctccccgc tgaccgctga ccgcctgggt gagctggcgc tggaagccgg
     1921 tattccggca ggcgtgctga acgtggtaca gggctacggc gcaaccgcag gggatgcgct
     1981 ggttcgtcat catgacgtac gtgccgtgtc gttcaccggc ggtacggcca ccgggcgcaa
     2041 catcatgaaa aacgccgggc tgaaaaaata ctccatggaa ctgggcggta aatcgccggt
     2101 gctgattttt gaagatgccg atattgaacg cgcgctggac gccgccctgt tcaccatctt
     2161 ctcgatcaac ggcgaacgct gcaccgccgg ttcgcgcatc tttattcagc aaagcatcta
     2221 cccggaattc gttaaacgct ttgccgaacg cgccaaccgt ctgcgcgtgg gcgatccgaa
     2281 cgatccgaat acccaggttg gcgcgcttat tagccagcag cactgggaaa aagtctccgg
     2341 ctatatccgt ctcggcattg aagaaggcgc aaccctgctg gcgggcggcc cggataaacc
     2401 gtccgacctg cctgcgcacc tgaaaggcgg caacttcctg cgcccaaccg tgctggcaga
     2461 cgttgataac cgtatgcgag tcgcccagga agagattttc gggccggtcg cctgcctgct
     2521 gccgtttaaa gacgaagccg aaggcttacg tctggcaaac gacgtggagt acggcctcgc
     2581 gtcgtacatc tggacacagg atgtcagcaa agtgttacgc ctggcgcgtg gcattgaagc
     2641 tggcatggtg ttcgtcaaca cccagaacgt gcgtgacctg cgccagccat ttggcggcgt
     2701 aaaagcctcc ggcaccgggc gtgaaggcgg tgagtacagc ttcgaagtgt tcgcggaaat
     2761 gaagaacgtc tgcatttcca tgggcgacca tccaattccg aaatggggag tctgatatgg
     2821 gtaagttagc gttagccgcc aagatcacgc acgtaccgtc gatgtatctc tctgaactgc
     2881 cagggaaaaa ccacggttgc cgccagggcg cgatcgacgg gcataaagag atcagcaagc
     2941 gttgccggga aatgggcgtc gataccatta tcgttttcga tacccactgg ctggtcaaca
     3001 gtgcttatca catcaactgt gcagaccatt ttgaaggcgt ctacaccagc aacgagctac
     3061 cgcattttat ccgcgatatg acctacaact acgagggcaa cccggagttg gggcagctta
     3121 ttgccgatga agccttaaag ctcggcgtgc gggcaaaagc gcacaacatt cccagcctga
     3181 aactggaata cggcacgctg gtgccaatgc gctacatgaa tgaagacaag cacttcaaag
     3241 tggtttccat ttcagctttc tgcactgttc acgattttgc cgacagccgc aagctgggcg
     3301 aggcgattct gaaagcgatc gaacagtacg acggcaccgt ggcggtcctt gccagcggtt
     3361 cgttatcgca ccgctttatt gacgatcagc gagcggaaga agggatgaac agctacaccc
     3421 gcgagttcga ccgccagatg gacgagcgcg tggtgaagct gtggcgcgaa ggccagttca
     3481 aagagttctg caatatgctg ccggagtacg ccgactactg ctacggcgaa ggcaatatgc
     3541 acgacacggt gatgctgctg gggatgctcg gctgggataa atacgacggc aaggtggagt
     3601 ttatcaccga gctattccca agctctggca ccggtcaggt taacgctgtt ttcccgcttc
     3661 ccgcgtaagg acgttttatg ccgcacttta tcgtggaatg tactgaaaat attcgtgaag
     3721 aagcccgctt acctgagctg tttgccagtg ttaacactgc actggcggca acggggattt
     3781 ttccgcttgg gggaatacgt agccgggcgc actggatcga tacctggcaa atggctgatg
     3841 gcaagcacga ttacgccttt gtgcatatga cgctaaaaat cggcagcggg cgcagcctgg
     3901 aaagccgtca ggaagtgggc gaaatgctgt ttgatctgat taaaacgcac ttcgcctcac
     3961 tgatggagag ccgttacctg gcgctttcat ttgagattgc agagctgcac ccgacgctga
     4021 attttaaaca aaacaacgtg cacgcgttgt ttaaataaat ttgcaccagc ttccaggccg
     4081 gatgataagg cgcagccgca tccggcaatg ccacaggata tcgctatgtt cgataaacat
     4141 acccacaccc tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc
     4201 gcgatctcac tggattaccc ggagatcacc atcgaagacg cttacgcggt gcagcgcgaa
     4261 tgggttcgcc tgaaaatcgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc
     4321 tcgaaagcga tgcaggcaag ctcgcagatc agcgaaccgg attacggcgc gctgctggat
     4381 gacatgttct tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt
     4441 gaagtggagc tggctttcgt gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc
     4501 gacgtctaca acgccacgga ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc
     4561 cacaacatcg atccggaaac ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac
     4621 gccgccaacg ccggggtgat cctcggtggt cgtcccatta agcccgatga gttggatctg
     4681 cgttggatct ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct
     4741 ggtgtgctga atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat
     4801 gacgtacaac tggaagccgg gcaaatcatt ctcggcggtt cgttcacccg cccggttccg
     4861 gcgcgtaagg gcgacacctt ccacgtcgat tacggcaaca tgggctccat tagctgccgc
     4921 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgccc
     4981 gcagatcgga ttatggctgg ggctgagtag cagctacagc gcagagttac tggccggagc
     5041 aggattcgac tggttattga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
     5101 cacccagcta caggcgattg cgccctaccc cagccagccg gtggtacgtc cgtcgtggaa
     5161 cgatccggtg caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat
     5221 ggtacaaaac gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg
     5281 tattcgcggt gtgggcagtg cgctggcccg agcctcgcgc tggaatcgta ttcctgatta
     5341 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
     5401 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
     5461 ggatctgagc gccgatatgg gttatgccgg taatccacag cacccggaag tacaggccgc
     5521 gattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
     5581 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ctgtcggcgt
     5641 tgacaccacc ctgctcgccc gcgccgctga agcgctggca gcacggtttg gcgcacaggc
     5701 cacgaccgtt aagcccggcg tgtattaatg ccggatgcgc tgcacttatc cggcctacac
     5761 tcgcaccaaa ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca
     5821 aatcgcggac cggataaggc gtttgtgcgc atccgccata aaccttgtcg taccctacaa
     5881 aaatcccatt agaggaagaa aaatgagcga cacctcacct gccataccgg agagtatcga
     5941 tccggcgaat cagcataaag cgctgactgc cggacaacag gcggttatta agaagctgtt
     6001 tcgccgcctg atcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa
     6061 catcggcttt gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg
     6121 cctcgctacc accctgttct atgccgctta tgtcatcttc ggcattccca gcaacattat
     6181 gctgagtatt gtcggtgcgc ggcgctggat cgccaccatc atggtgcttt ggggcatcgc
     6241 ctctactgcc accatgtttg ccactggccc caccagcttg tacgtactgc gtatactggt
     6301 tggcattacc gaagccggct ttctgcccgg cattctgctg tatttaacct tctggtttcc
     6361 ggcctatttc cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc
     6421 gttgggctcg atcgtttccg gctacatttt gtcgctggat ggcgtcatgg cattaaaagg
     6481 ctggcagtgg ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg
     6541 gttctggctt gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg
     6601 cctgcaagag atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag
     6661 ccaccacgcc atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat
     6721 gtataccctg gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc
     6781 gcagatcctg caaagtttta atcagggcag cagtaatatc accatcggcc tgctggccgc
     6841 cgtaccgcag atttgtacca ttctcgggat ggtctactgg agccgtcact cagatcgccg
     6901 ccaggaacga aggcatcaca ccgcccttcc ttatttgttc gctgccgcag gttggttact
     6961 ggcttcggca actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg
     7021 atcattcagc gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc
     7081 acgagcgatc ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc
     7141 gtttatgatc ggctggttga aagatttgac cggcagcttt aacagtggat tgtggtttgt
     7201 tgccgcgctg ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg
     7261 tccgcgagcg accccgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat
     7321 cagcaaagag tacgatgaaa gcctgggcac ggacgatgtg cattatcagt ccttcgcccg
     7381 catggcggcc ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat
     7441 gcatttcctc aatagcggac agattgagct acaacttgac gatcatcgct actcggtgga
     7501 agcgcccctg tttgtcctga cgccgccgtc agtacctcat gcgtttatta cggagtctga
     7561 cgccgacggt catgtgttga cggtacggga agatctgatc tggcccctgc tggaagttct
     7621 ttatccgggc actagggaaa ccttcggcct gccggggatt tgcctgtcac tggcagataa
     7681 acccgacgaa ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtgga
     7741 acaactgcct ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact
     7801 gctgcgtaac gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact
     7861 gttccagcgt tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga
     7921 ttacgctaac gaactgcata tcaccgaatc acgactcacg gacatctgcc gtcgctttgc
     7981 caaccgtccg ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct
     8041 gctgttttct gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc
     8101 ttattttgcg cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa
     8161 aaaagtacct gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta
     8221 ttcccctttc tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac
     8281 ctcgatcaca aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc
     8341 ccgtaatcgc ccctgcaagc ttcaattgaa taacaaaaac aacacacaaa attaacaatc
     8401 gtattccgat taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc
     8461 aacgtccgtt caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata
     8521 tctatggcga gcgagtgaaa gacgtcacta ctcatccggc atttcgtaat gcggcagcgt
     8581 ctgttgccca actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga
     8641 acaccgacac cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgccg
     8701 acgacctgcg ccagcaacgc gatgccatcg ctgagtggtc acgcctgagc tatggctgga
     8761 tgggccgtac cccagactac aaagctgctt tcggttgcgc actgggcgcg aatccgggct
     8821 tttacggtca gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc
     8881 tctactttaa ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag
     8941 tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc gttagcggtg
     9001 cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg
     9061 cacaagtaat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgcca
     9121 atggcgtcaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc
     9181 cgtacgacta cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata
     9241 acgtgctgat cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct
     9301 ggacgatgga aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag
     9361 tgaaactaga cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg
     9421 agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg
     9481 cattgagtga ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg
     9541 atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa
     9601 acattatcga acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc
     9661 tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg
     9721 atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg
     9781 gtggtcgcca cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc
     9841 agtgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc
     9901 gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg
     9961 atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga
    10021 tgaacagcgc ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat
    10081 caccaccgag ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac
    10141 ggatacacca ccatcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt
    10201 tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg
    10261 ccacttcgcg ggcatgacgg gcatggcgat ggaagagcgt tttagcctct catgctggca
    10321 aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggtgagat
    10381 ccgcgatgtg caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat
    10441 cctcagtgca gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cggtgatgct
    10501 ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt
    10561 tattgtgatc tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc
    10621 attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgcg
    10681 cgtaactctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca
    10741 aacctagaac ggcttcggcc tatgattaac aaattactct atgaattttg gtttgcagga
    10801 aggcagcaag tgagtgaatc cccgggagct tacaacagta agtgacaggg gtgaacgaac
    10861 gcaactgccg cacctgtaag ctaaaagacg acgagtaaaa tgccaggttt tactatggat
    10921 acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc
    10981 ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc
    11041 tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg
    11101 atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac
    11161 gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt
    11221 ccgctggtcg gtccggttct cgccgcacag atgggctacc tgcctggcac gctgtggctg
    11281 ctggcggggg tagtactggc cggtgcggtt caggacttta tggtgctgtt tatctcctct
    11341 cgccgtaacg gcgcatctct cggtgagatg ataaaagaag agatgggacc agtaccgggg
    11401 actatcgcgc tgtttggctg tttcttaatc atgatcatca tccttgccgt cctggcgctg
    11461 attgtggtta aagccctggc cgaaagcccg tggggtgttt tcaccgtttg ctcaaccgta
    11521 ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa
    11581 gtctctgtca ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct
    11641 cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg
    11701 attggctatg cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac
    11761 tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tattgtggtg
    11821 ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg
    11881 tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc
    11941 ttccacgcgc tgatctcttc cggtacgact ccgaaactgc tggctaacga aaccgacgcg
    12001 cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt
    12061 gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt
    12121 ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg
    12181 cagctgaaag acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt
    12241 tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt
    12301 gcaggtggtg cgccaacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg
    12361 atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg
    12421 accgcgctgg atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac
    12481 ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc
    12541 tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt
    12601 aagagcctgt ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta
    12661 ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt
    12721 ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc
    12781 aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac
    12841 ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac
    12901 accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt
    12961 ttcaaaacct ggctgaatgt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac
    13021 gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgta tttagccccg
    13081 cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa
    13141 aaaatatctc ggccaggcgg cgaagatgtt gattggcatt ccagactacg acaactacgt
    13201 cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg
    13261 cgaacgccag aacgcacgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg
    13321 gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa
    13381 ccaccctgct gcgccatatt cttaatgaac aacacggcta caagattgcc gtgattgaaa
    13441 acgaattcgg cgaagtctcc gttgatgatc aattgattgg tgatcgcgca acgcagatca
    13501 aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcgctac
    13561 tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg gtcattgaat
    13621 gcaccggcat ggccgatcct ggcccgatta ttcaaacctt tttctcccat gagattttat
    13681 gccagcgcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc
    13741 agatgaacca gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga
    13801 ctaaaaccga cgtcgcaggc gaagcagaaa aactgcgtga acgtctggca cgcatcaacg
    13861 cccgtgcacc ggtctacacc gtcacccacg gcgacatcga cctgggtctg ctgttcaaca
    13921 ccaacggttt tatgctggaa gaaaacgtcg tcagcaccaa accgcgtttc cattttatcg
    13981 cggacaaaca aaacgatatt tcgtcgattg tggtggaact ggattacccg gtagatatca
    14041 gcgaagtttc ccgggtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt
    14101 acaaagggat gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc
    14161 gcctctacag cgccgactgg gacaggccat ggggcgatga aacaccgcat agcacgatgg
    14221 tgtttatcgg gattcagttg ccggaggatg agattcgggc ggcgtttgcg ggactcaaaa
    14281 agtaaccagc cctccaacat cacaatacct gagactttat caccctcata ttcctccaga
    14341 atatgagggc tttcgggctt aattatctct gaacgtgcaa cacttcctgc gactccttat
    14401 gcgcctccac atcctgcttc gttaaccaga gcgacttacg gccaaaattt tcgtacattt
    14461 tcagctgatc ttcatagtgc ttatcaactg ttccatcggg agcaataaac ccactctgac
    14521 cgggtgcgac cacatcccag gcaagcacag gacgatcgct tgtcgttggt gagaaaacaa
    14581 tcatatcgtt ttctgttcca cggttttgat actccgcctg atgacgcgtt tcttccgctg
    14641 cggcctgcgg tacaccaaag aaattatttg cccggaacgt taaggccatt gcaggtgttt
    14701 tccagttact cacattattg ccatagcgtt tggaaagagt ctcccaggta tcttccagcg
    14761 cagccaacac aacctcctgc tgtggtttcc cagcaaacag atcaaccgcc tgtgggattg
    14821 gtgatttgtc tccctgcacc gcctcataca aaatttttgc tccaacactt atattcagcg
    14881 aaccagttgg gccgtcctgg gttgtttcgt agccactggc gctgtaccac ttatcaaatg
    14941 gcataggtac ggcagccact acggtacgct tcaacatact ggtcagccaa acgttcagga
    15001 tggcagagcc tggctgctgc caggttttac catcatcatt aagcaaattg atgccatccc
    15061 aacgtgttaa tgtttctacc aactgacgac gcggatcgct ctgtgtcaaa ccagatgtcg
    15121 ctgcttgcag agtaggtaaa aaaagcctca ggttaagatc ctgacgactg gtttggcgaa
    15181 taacatccca tgcctgatca gcagttaagc gtggcttttg ctcaagcagt cggtcgatct
    15241 ccgtaacgcg atctgcacca ccccacaaaa aggcaaacag atctgaagcg ggataatctt
    15301 tttggggaga attgttccag ttagcaatat atcccgactg ggggttatac accttagggt
    15361 tcatttcaaa aggcaatagc cctttccagt cccattttcc cgtaccagga acgggtaatc
    15421 gcggatcatg gcctgattga cgatctggat aagcaccagt atgaacataa ccaatattgc
    15481 cgtttacatc agcatagtac cagttgatgg tcagtgcttg tttcgctgcc tgctgtgtcc
    15541 actcctgcca atttttggcc ttcatctgat gagtccaggc cagcaaagac gccacctctt
    15601 taccatccca tgcgcgggat ttagcgtaag ccgtttgtgt cgtctggtca gtttggagaa
    15661 tgttgccatg caccgtacgc cagacagtaa aggtctctgc ctgaccattt ttcaccgtaa
    15721 tggtttcctc acggcttaac attttcaccc acttaccatt atgcaagtag tagcctggtt
    15781 tctctgccga cagccgttca gcaaaaatat cgacatcatc gccgaaacct gccgttgatc
    15841 cccaggaaat cacaccatta tgaccaaaaa ccagcccagg ataggcaaat ggtgtattgc
    15901 cagtgacatc ataaccagca ccgtgcagac caataccata agtatacgca ggcgcatacc
    15961 agccaaactg cggaccattt accatgattg ctttcgcatc ctgggctttg cttttgccga
    16021 tcacccacat attgctggtc gttggatacc ccgccagacc attggcacca ccctgtgcaa
    16081 attgtgcagc aatagtttcc cggttcttcc ctgctgttaa cgccagcagt gcgccatccg
    16141 ccccttttgc tggtcggtca agcattggtg caggtaaatc gtagcgtggc aacagagctg
    16201 ctgtttgcga gttttgctga ttaaatttaa gtgggtagtt actctcttgt acggcaatag
    16261 tggttggcgc tgatgggttt accagccatt tcaactgatt aaataccgcc atgccttgtg
    16321 atacaccata tttatctttt aaagccgtta gcagtgccaa attatcaatt tcgctagtgc
    16381 tatcagagaa gcggtttgcc atggtgccca caaatatcat cgcgacatca aacggttccc
    16441 agcgcttagg agtaaagcca aatgtattaa actgttttgg taagagcgtc tctggattgg
    16501 tatttacctt atcaatccag gcattcattc catcagcgta gccttgcaga atggacatat
    16561 cctctgggga aagggcagca atttgcgccc ggatagcatc cggccagtag ttacgacgga
    16621 tatctttatc aaatttcaca aaatctttgc caagcacttc cgcgacagtc ccttgagtac
    16681 tgcgacgtgc catttccatc tgaaaaaggc gatcttgtgc tactacatag ccatagccat
    16741 aaaataggtg ccatgtatca ttggcataaa tatgcggcat gccgtattca tcgcgaacaa
    16801 tctttatctc acttgacgac tgctcagcca gtgcaggtaa gctccaataa tacatcaggg
    16861 aagcagtaac acagttcacg atcatacgat ttctattttt cattgtatcc tctggcaggt
    16921 gtataattag cgaattgata ctagcaacga agcttcggtt atctatttgc agagaattgt
    16981 ttcattatga actgtgagcg cgataactaa tttttgatac aaaaatttca ttaatgtgat
    17041 ctaaaacgaa tcgctttatt caccgctgat gattttgttc tattcctttt tgtgacagat
    17101 cccccggacg catagatgat gacccccaga gagacagctc aggtcattat ctgtatgtaa
    17161 atcagaaaaa tatgttgtaa acgactttta tggttggatt ttgtagcaat tttgaatgca
    17221 gacaggcagc atttgccaac acgccgccca tagttcactc cacatcctcc ctgcgtaacc
    17281 caatatccct caaccgttca tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc
    17341 gccgccaacg cctcaccgca tgccagagtt gcacaagtct gataaacggc gttttggctt
    17401 tgttttcgtg aaattccatc tgctgcctcc tcatctttca gaggcttcat catccgcctt
    17461 ctggcagttc acaatacaga tgcaaatatt agttttattt aacatacaga caggttaaaa
    17521 cagcatctgc atccggtttt ttagcctcat ctgtaccggt ttttctttct gtatgggcca
    17581 aataaggaat acagcatgac gcgttatcaa catctggcga ctctacttgc cgagcggatt
    17641 gagcaagggc tgtatcgtca cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag
    17701 cacggcgtca gcatcagcac cgtgcaacag gcgtatcaga cgctggaaac gatgaggctc
    17761 atcactccgc agccgcgttc gggttatttt gtcgcacaac gtaaagccca gccgcctgta
    17821 ccgccgatga cgcgtccggt gcagcgcccg gtggaaatta cccagtggga tcaggtgctg
    17881 gatatgctgg aggcgcatag cgacaattcc attgttccgt taagcaaaag cacgccggat
    17941 gtcgaaacgc ccagcctgaa accgctgtgg cgtgagctaa gccgggtggt gcagcataat
    18001 ctgcaaaccg ttctcggtta tgacttgtta gccggtcagc gagtgttgcg ggagcagatt
    18061 gcccgcctga tgctcgacag cggctcggtg gtcaccgccg atgacatcat catcaccagc
    18121 ggctgccata actcgatgtc gctggcgtta atggcggtgt gtaaaccggg cgatattgtc
    18181 gcggtcgaat ctccctgtta ttacggttcg atgcagatgc tgcgaggcat gggcgtgaaa
    18241 gtgattgaaa tcccaaccga tccagaaact ggcatcagcg ttgaagcact ggaactggcg
    18301 ctggaacagt ggccgattaa aggcatcatt ctggtgccaa actgtaataa tccgctggga
    18361 tttattatgc cggacgcacg caaacgggcc gttctctctc tcgctcagcg tcatgatatt
    18421 gtgatttttg aagatgatgt ctacggcgaa ctggcaacgg agtatccgcg cccgcggacc
    18481 atccattcat gggatatcga cgggcgagtg ttgttgtgca gctcgttcag taaaagtatt
    18541 gctccaggcc tgcgcgtggg ttgggtcgca ccggggcgtt atcacgataa actgatgcat
    18601 atgaaatacg ccatcagcag ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt
    18661 gtgctggaag gtcactatca tcgccatatc cggcggatgc ggcagagcta tcagcgcaat
    18721 ttggcgcttt atacctgctg gatacgggaa tattttccct gcgaaatctg tattacgcgc
    18781 ccgaaaggcg gatttttact gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtt
    18841 gcgcggcagc tgtgccgcat gaatatccag gtggcagcag gctcgatttt ctcggcttcc
    18901 ggcaaatacc gtaattgtct acgcatcaac tgcgctttgc cgctcagcga aacctatcgc
    18961 gaagcactaa agcaaattgg cgaggccgtg tatcgggcaa tggaataaat tcgaattagt
    19021 ggcgaagcac ctgatttcct ctcacgaatc gcctgtaact cgttgtatct ttgcagggtc
    19081 atcgccacgt tccagcatgg cttaggcaaa gtgtgcttcg ttcataccgg atgcggcgtg
    19141 aacgccttat ccggcctaca aaccgcgcaa attcaataga ttgcagagga aatgtaggtc
    19201 tgataagcgt agcgcatcag gcagctttgc gtttgtcatc cgtctctgat tagtattcga
    19261 tccatggatc gcgcttgatt tcaggcaagg gctgctctaa tgcctgcatt tgctctgtcg
    19321 aaagtccggt aatcttttgt atccattcag gatctgcccc attctgcaac aacaatctaa
    19381 ggaggcgttg ttccccttct tctttcccct tttccatccc caacagccat ccatcattat
    19441 aaattcgctc tgcaatcgtc attattcgct ccctgtgttg tggcattcga cgggtaagtt
    19501 cgctgataaa cttcttaaaa cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg
    19561 cagttatctg gctgtcatta gcacactctg taaccagtaa tattaccagt tgatcgataa
    19621 gccccatcag atcgcgctgg cgaatatgct tttggatcaa ctccaacagg gcgactctgc
    19681 ggtgctgcac aatctcgtcg tctggcacga cagtgacatc caccagcggg aacgctgcgg
    19741 tataaagctt ccgtgcggtg gtcgggtcag caaattcgtc cagccagcac agggaccagg
    19801 gataaggact acggctaccg tgataaaaca gcatcgggat gaccagcggt agcggccggc
    19861 gtttatcatg ctcgatatgg cgctgcatca ccgccatgga atatcgcatc aggcgaaagg
    19921 ccatatggat atcctcgcgg ctctgatgtt caatcactac gtaaatataa ccatcacctt
    19981 cacgggtctt taccgaccac agaatatcgg agtgtagcgc ccgcaatttt tcatcgacga
    20041 agctggcgga ttccagtttt aagctgtcga gatcgcacag ttcacgtaaa tctttgggta
    20101 agtggatctc cataaaatcc cgcgcggtgt cagggtgcgt gagaaaggat ttaaataatg
    20161 cgtcatgcgg cgtgctggtt gtgaagtttg tcatggcgat ccgtcacctg tagataagat
    20221 ggcccgactg tagcgactaa caaaaccatt ctcacccggc aatttctcgt tccccgaggc
    20281 gctttccagg ctatttgtgt tcctctgcaa acgatacatt tttccggaaa caggctcgcg
    20341 ttttacattt cctgtacccg gcagatattc ccctttccac cgcgctcttt tgattaaccg
    20401 caaattttaa ttacacttaa ggtgtatatt ttctatgcaa cccatcaatt aaagaggtgt
    20461 aatgtgctga tgactatttg taatcgttat acattctgac ctggagtcag aaggtatttc
    20521 tctgtctgtg tgttcacagg cagtgtggtt gattacatga aattcagtac atttgtcctt
    20581 ctcgcctgcc tttcgtcatt accgacggta ttgaatttcg ttttccccgt tggggttctc
    20641 cggacaagga gttgtttgtt atgccacgtt tttttgcccg ccatgccgct acgctgtttt
    20701 tcccgatggc gttgattttg tatgactttg ccgcctatct ttcgacggat ctgatccagc
    20761 cggggatcat taatgtggtc cgtgatttta atgccgatgt cagtctggct ccggctgccg
    20821 tcagtctcta tctcgcgggg ggtatggcgt tacagtggct gctggggccg ctttccgaca
    20881 ggagtggccg caggccggtg ctgattaccg gggcgctaat atttaccctt gcctgcgctg
    20941 cgacaatgtt cacaacgtct atgacacagt ttcttatcgc gcgtgcaatt cagggcacca
    21001 gtatctgttt tattgctacc gttggttatg tcacggtgca ggaggcgttc ggacagacaa
    21061 aagggattaa gttgatggcg attatcacct ccatcgtact gattgcgccg attattggcc
    21121 cgctttccgg cgcagctctg atgcactttg tgcactggaa agtccttttt gccatcattg
    21181 cggttatggg gtttatctca tttgttggct tactgctggc gatgccagag acggtgaagc
    21241 gcggcgcggt tccatttagc gccaaaagcg tcttgcgcga ttttcgtaat gtcttttgca
    21301 accggctgtt cctctttggc gcagcaacca tctctttaag ctatatcccg atgatgagtt
    21361 gggtggctgt ctcgccggtg atcctgattg acgcaggcgg cttaacaact tcgcagttcg
    21421 cctggacaca ggttccggtg ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt
    21481 tcgttaaaga tccgaccgaa ccgcgcttta tctggcgtgc tgtacccatt caactggtcg
    21541 gcctcgcgct gttgattatc ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc
    21601 tgggcaccag tttgtatgct ttcgggattg gtttgatctt cccgacctta ttccgcttta
    21661 cgcttttttc caataactta ccgaaaggaa ccgtctccgc atcgctgaat atggtgatcc
    21721 tgatggtgat gtctgtctcg gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct
    21781 tgccgtttca tctgttagcc gtcgtggcgg gcgttatcgt cgttttcacc ctggcaggat
    21841 tgctcaatcg cgtgcgccaa catcaggctg ccgagctggc ggaggagcgg tgatttttgc
    21901 gcgatcctgc cgtcaggctc tattcttaac gttatgaata aactcattga actcagacgc
    21961 gccaaaatgt tggcgctctc tttactgctt atcgccgctg ctacctttgt cgttacgctg
    22021 tttttgccgc ccaatttttg ggtgagcggc gtgaaggcga ttgctgaagc ggcgatggtc
    22081 ggcgcgctgg cggactggtt tgcggtggtg gcgctgtttc gccgcgtgcc gattccgatc
    22141 atttctcgtc atacggcgat tatcccgcgt aataaagacc ggattggcga aaatctcggc
    22201 cagttcgtgc aggaaaaatt tctcgatacc cagtcgctgg tggcattgat tcgacgtcac
    22261 gaaccggcgt tgttgattgg caactggttt agtcagccgg agaacgcccg ccgcgttggt
    22321 cagcatctgt tgcagatcat gagcggtttt cttgaactga ccgatgatgc gcgtattcag
    22381 cgcctgctta agcgcgcggt ccatcgggcg attgataagg tcgatctttc cggaaccagt
    22441 gcgttgatgc tggagagtat gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg
    22501 atcgcacagt tgatcgccct tctccagcgc gataaatcgc gcaagtttat tgcccagcaa
    22561 attgttcgct ggctggagag tgagcatcca ctgaaagcca aaattctccc caccgaatgg
    22621 ttgggcgaac atagcgcgga gttggtttct gacgcggtga attctttgct tgatgatatc
    22681 agccgtgatc gtgcgcatca gatccgttat gcgtttgatc gcgccacttt tgccctgatc
    22741 gacaagttga aaaacgatcc ggaaatggca gcgcgagccg atgccgtaaa aagttatctg
    22801 aaagaagatg aagcttttaa ccgctatctc agtgaattgt ggggggattt acgggagtgg
    22861 ctgaaagcgg atatcaacag tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt
    22921 caatggtttg gcgaaacgtt aattgccgat gatgccttgc gggcgtcgtt aaatggtcac
    22981 ctggaacaag ccgcgcaccg cgtcgcgcct gagttttccg cattcctgac gcgccatatc
    23041 agcgatacgg taaaaagctg ggatgcgcgg gatatgtcgc ggcaaataga gttaaatatc
    23101 ggcaaagatc tgcagtttat ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta
    23161 attttatatt tgttgtcgca gctcccggcc ttgttccccc tcggcaattt ttagaaatcc
    23221 atgaaaaact aagtagctaa ccgcgagaga gatcaaataa accacactaa aagaatggta
    23281 ataatgtggc ctcttttcat tatctcccgt ggtacgggga aggaaaatca tgtcacttgt
    23341 caccgatcta cccgccattt tcgatcagtt ctctgaagct cgccagaaag gctttctcac
    23401 cgtcatggat ctcaaggagc gcggcattcc gctggttggc acttactgca cctttatgcc
    23461 gcaagagatc ccgatggcag ccggtgcggt tgtggtttcg ctctgttcca cctctgatga
    23521 aaccattgaa gaagcggaga aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag
    23581 ctacggcttc ggcaaaaccg ataaatgccc ctacttctac ttttctgatc tggtggtcgg
    23641 tgaaaccacc tgcgacggca aaaagaaaat gtatgaatac atggcggagt ttaagcccgt
    23701 tcatgtgatg cagctgccga acagcgttaa ggacgatacc tcgcgtgcgt tatggaaagc
    23761 cgagatgctg cgcttgcaaa aaacggtaga agaacgtttt gggcacgaga ttagcgaaga
    23821 tgctctgcgc gatgccattg ctctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta
    23881 tcatcttggg cagttaaatc ctccggcgct tagcggcagc gacattctga aagtggttta
    23941 cggcgcaaca ttccggttcg ataaagaggc gttgatcaat gaactggacg cgatgacagc
    24001 ccgcgttcgt cagcagtggg aagaaggcca gcgactggac ccgcgtccac gcattttaat
    24061 caccggctgc ccgattggcg gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg
    24121 cggctgggtt gtcggttatg aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc
    24181 agaaacgggc gatgtctacg acgcactggc ggataaatat ctggcgattg gctgctcctg
    24241 tgtttcgccg aacgatcagc gcctgcaaat gctcagccag atggtggaag aatatcaggt
    24301 cgatggcgta gttgatgtga ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc
    24361 gattaaacgt catgtgcgtc agcagcacaa cattccttat atcgctattg aaacagacta
    24421 ctccacctca gatgtcgggc agctcagtac ccgtgtcgcg gcctttattg agatgctgta
    24481 aggagtggca gtgacatatt cgattggcat tgattctggt tctaccgcca ccaaagggat
    24541 cttactggca gacggcgtga ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc
    24601 aacagcaatt actgaagcct gggaaactct gcgcgaaggg ttagagacaa cgccgtttct
    24661 gacgctcacc ggctatggac ggcaactggt ggattttgcc gataaacaag tgacggaaat
    24721 ctcctgtcat gggctgggcg cacggtttct tgcgccagca acgcgcgcgg taatcgacat
    24781 cggtggtcag gacagcaaag tgattcagct tgatgatgac ggtaacctgt gcgatttcct
    24841 gatgaatgac aaatgcgcgg cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct
    24901 tggcaccagc gtcgagcaac tcgacagcat taccgaaaat gtcacgccgc acgccatcac
    24961 gagtatgtgc acagtgtttg ccgaatcaga agtgatcagc ctgcgctcag cgggcgtcgc
    25021 gccagaagcg attctcgccg gagtgattaa cgcgatggcg cggaggagtg ccaatttcat
    25081 tgctcgtctc tcctgtgaag cgccgattct gtttactggt ggcgttagtc attgccagac
    25141 gtttgcccgg atgctggaat ctcatctggg aatgccggta aatacccatc ctgatgcgca
    25201 atttgctggc gcaattggcg cggcggtaat tggtcaacga gtgaggacac gccgatgaaa
    25261 gagtttttat ttttatttca ctccacggtc ggcgtcatac aaacccgcaa ggcgttgcag
    25321 gcagcgggca tgacttttcg cgtcagcgat attccccgtg atttacgcgg cggctgcggg
    25381 ttatgcattt ggctgacctg tccccccggc gaggaaatac aatgggtgat ccctgggcag
    25441 actgagtcaa tttattgcca gcaggatggt gtctggcgct gcattgcaca ttacggggtt
    25501 tctccgcgtt aaggattcat taaggtcgct acgtatactt ctcgatatct tattgatttg
    25561 ccttaaaggg attaccgtga caaaaagtat ttcgttaagt aaacgcatct ccgttattgt
    25621 gattcttttt gccattgtgg cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag
    25681 caatcatgca gcatcatttc agaattacca cgccaccatt gacggcaaag aaattgcagg
    25741 cataacgaat aatatctctt cgctgacctg gtcggcacaa agcaatactc tttttagcac
    25801 tatcaacaaa cctgccgcca ttgttgaaat gaccaccaat ggtgatttta tccgcacaat
    25861 tcctctggat tttgttaagg atctcgagac gatcgaatac attggcgata atcagtttgt
    25921 cattagcgat gagcgcgatt acgccattta tgtgatttca ctgaccccaa actcggaagt
    25981 aaaaatcctc aaaaaaataa aaatcccgct gcaagactcc ccaaccaatt gcggttttga
    26041 agggttggct tattctcgcc aggatcacac tttctggttt tttaaagaga aaaatccgat
    26101 tgaggtctac aaagttaacg ggctgttaag tagcaatgaa ttacacatca gcaaagacga
    26161 ggcactacaa cggcaattta ctttagatga tgtgtccggc gcagaattta accagcaaaa
    26221 aaatacgctt ctggtgctat ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg
    26281 cgaggttatc ggtgaaatat cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa
    26341 acaggccgaa gggattgcga tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa
    26401 tcgtttttat cgctttaccc ctaaatcatc gcattaacag gctggcatat ttcacctttt
    26461 tgttattaac ttgttaacaa caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc
    26521 atcccgtaga acgcttttct ttcagcaccg cgcttttcgg gatgctggtt ctgaccttag
    26581 gtatgggatt aggtcgcttc ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg
    26641 cgttttcatt tagccagctc tcgtggattg ccagcggcaa ctatgcgggg tatctggctg
    26701 gcagtctgct attttcgttt ggcgcatttc accagccgtc gcgcctgcgc ccattactgt
    26761 tggtttctgc cctggcgagc gggttgttga tcctcgcaat ggcatggttg ccgccattta
    26821 ttctggtgtt attgattcgc ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg
    26881 gttcgacgct gattatgcag cacacgcgcc atccttttgt gctggcagct ttgttttctg
    26941 gcgttggcat tggcattgca ctgggcaacg aatatgttct ggcaggcctg cattttgcct
    27001 tatcctcgca aacgttatgg caaggcgccg gggcgctttc tggcatgatg ctgattgcac
    27061 ttacgctttt aatgccctcg aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg
    27121 agcaacagat aatgagctgg tggttactgg ctattctgta cggcctggcg ggatttggtt
    27181 atatcatcgt cgcgacttac ctgccgctca tggcgaaaga tgcaggctca ccattgttaa
    27241 ccgcccatct ctggacgtta gtcggcttat caattgtgcc aggctgcttt agctggctat
    27301 gggcagcaaa acgttggggg gctttgcctt gcctgacggc gaatttgctg gtgcaggcta
    27361 tcagtgtgct gcttactctc gccagcgact ctcctctctt gcttatcatt agtagtatcg
    27421 gttttggcgg tacctttatg ggcacgacat ctctggtgat gactatcgcc cgccaactga
    27481 gcgttccggg aaatctcaat cttttaggct ttgtgacgct catttacggc attggacaaa
    27541 tcctcggccc ggcgctgacc agtatgctcg gcaatggaac atcggcgctt gccggcgcca
    27601 ctctctgcgg cgcggcggcg ttgtttattg ctgcgcttat cagcacagta caattattta
    27661 agctgcaagt ggtcacttca tgaggagtgt ttcccttacc actcaagaaa ttcgaccgca
    27721 accgttgatg tcagccagac tccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
    27781 atttcctgtg ctttgacggt taagataacc ggcgatccgt gtcttgctcc cactttgcgt
    27841 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
    27901 tttttatctc gtcaaggaaa cggcttgccg taccgtggta gagaaactgt ggcggtgttt
    27961 tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg cggatgcacc
    28021 taccatcact ggaataacta aaacgctttt tatcgctggt tgcgaccacg gcatccagca
    28081 atgcacgggt taaccgcttg cccgcttttt gggcacagag tataagctta tcgatatccg
    28141 cccatccttc acggtccagt acgataccaa tcgcctccgg tttgtgtcgg aggacaaaac
    28201 ttaaaaactt gctcgtatcc gccagctctt tttcgttgta ctttgccatc gcgttttcgt
    28261 ccctggattt tctgttttta tttactgatg tcattgtcac tgggtttatg aattttctgc
    28321 aactcaatcc tgcgcagagc cttttccaca cagcgtaatt ttaccgctat gcaaaaaacg
    28381 catagccaga aacatacttg cattttcccc atacccacat ggctgataag gtttttccgc
    28441 tccaggaatt ttcctggctc aagcaacaca caaacacaaa aaaacaatgg ggattgttat
    28501 gacacaacaa ggggatgctg tagtcgggga gctggcgaca gaaaaagtgg gcattaaagg
    28561 gtatctggct ttttttctca ccataatctt tttttccggt gttttttccg gaacagacag
    28621 ctggtggcgc gtttttgatt tttccgtgct gaacggatcg tttgggcaac tgcctggtgc
    28681 gaatggagcc accacctcat ttcgcggggc gggtggtgcg ggggctaaag atggttttct
    28741 ctttgcgctg gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga
    28801 tggtcttggt ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt
    28861 gggcattcca gggatctgct cgctggcgct gatcgctaac ttgcaaaaca ctgatgccgc
    28921 cgccggtatg accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat
    28981 tttcgccgca tatcaaacca gcggtagcgc catcatcacc aactacttct cttctggcgt
    29041 cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt
    29101 taaatttatc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc
    29161 aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc
    29221 gatggcgcgc gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg
    29281 gcgtttgtca tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc
    29341 tgtgaacccg taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc
    29401 gcattaatga gtatgggtgg cgcagtcggt gttgccgcta gtctggcaac cgctggcgca
    29461 ataaccggtc acgatgtcac cgttttactg cccgcgatgt atttaatggg caacccggtg
    29521 caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc
    29581 attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa
    29641 caaggagtta ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat
    29701 ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc
    29761 gccgttgcca gcaatatccc ttctgacatt gtaccggact gcacggttgt cgatctcagt
    29821 gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc
    29881 gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc
    29941 gtcacgtcag tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg
    30001 ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct
    30061 tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat
    30121 cgtgtgattg gcgtgaaatg cgccatatct gatcaccgtt ctgccgcacc ggacgtttat
    30181 cacctggcta atatggcggc ggaatcccgc gttggcggtt tgctcggcgg taaacctggc
    30241 gtcaccgtgt tccacatggg cgacagcaaa aaggcgttac agcctgtcta tgacctgctg
    30301 gaaaactgcg atgtgccaat cagcaagttg ctgccgaccc acgttaaccg caacgttccg
    30361 ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc
    30421 attgacgaac cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg
    30481 ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa
    30541 gggaatttaa cccatatcgg tgttgccggt tttgaaacgt tgctggaaac cgtgcaggtg
    30601 ctggtcaaag actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta
    30661 gccggtttcc ttaacctgac cgggaaaggc gaaattctgc caggcaatga cgcagacttg
    30721 ctggtcatga cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc
    30781 aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cataaacggt tgtcagccgc
    30841 ccgaatggac gtagaatgcc cgcaagggcg gctgacagag taaaacctaa tggatgactg
    30901 tggtgcggtt ttgcataata ttgaaaccaa atggctttat gattttctga ccctggaaaa
    30961 atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg
    31021 ccgcatccgt gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc
    31081 actgcaactc tcggaacaag ggaaaatctt tcattcgcag atccgccatc tgttgcaaca
    31141 gttagaaagt aacctggcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa
    31201 gatagccgct gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc
    31261 gccgctcttt acctgggcaa ttgaagccat tgatgttgat gaagcggtcg ataaactgcg
    31321 tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt
    31381 tgaccacatt cgcttatttg aatctcaatt gttccccgtt tgcgccagtg acgaacacgg
    31441 agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc
    31501 ctacatgggg cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac
    31561 cttttttgtc tcttcgatga gcgagctatt aaagcaggtt gccctcgacg gctgtgggat
    31621 tgcctggctg ccggaatacg ctatccaaca agaaattcgc agcgggcagc tcgttgtgct
    31681 gaatcgggac gaactggtga tcccgatcca ggcctacgca taccggatga atactcgcat
    31741 gaatcccgtt gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg
    31801 acattctcca gcatcgcact acaccaaccg ccattatccc agaccagatc aaacgttagc
    31861 ggttcatcgt cgtggtacag cattatctca aggctgatcg ccagcgtgtg gctgtacgct
    31921 tctttcagat tgacctgcaa cgctgcaatc cgtggctcat gaaataacag tgcagaacgc
    31981 agctgacggc aataccagct ctggagatgt ttgtcgctct ggttatgcac ttgccactcg
    32041 ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg
    32101 agatcgctgc ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa
    32161 acagcctgaa gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg
    32221 cttgccctga acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt
    32281 gttcattaca tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc
    32341 ttacttgcct atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc
    32401 atcaaactca aaaaaaaacc gctagtatat tctgaatatt ccgaatagtt acgtagatga
    32461 aaatacttgt caccgccgct acttaattcc ggtgggaaat tat
//
LOCUS       DAPPNJ010000029        32099 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000029 DAPPNJ010000000
VERSION     DAPPNJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.029"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            167..466
                     /locus_tag="RPM73_001077"
     CDS             167..466
                     /locus_tag="RPM73_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519460.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVLPVTNIVLFLILIFFKKR"
     gene            complement(513..1400)
                     /gene="rarD"
                     /locus_tag="RPM73_001078"
     CDS             complement(513..1400)
                     /gene="rarD"
                     /locus_tag="RPM73_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDX6519461.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1452..1919)
                     /locus_tag="RPM73_001079"
     CDS             complement(1452..1919)
                     /locus_tag="RPM73_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDX6519462.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2084..2953
                     /gene="pldA"
                     /locus_tag="RPM73_001080"
     CDS             2084..2953
                     /gene="pldA"
                     /locus_tag="RPM73_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDX6519463.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLE
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3086..4915
                     /gene="recQ"
                     /locus_tag="RPM73_001081"
     CDS             3086..4915
                     /gene="recQ"
                     /locus_tag="RPM73_001081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDX6519464.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4979..5599
                     /gene="rhtC"
                     /locus_tag="RPM73_001082"
     CDS             4979..5599
                     /gene="rhtC"
                     /locus_tag="RPM73_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDX6519465.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5661..6281)
                     /gene="rhtB"
                     /locus_tag="RPM73_001083"
     CDS             complement(5661..6281)
                     /gene="rhtB"
                     /locus_tag="RPM73_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDX6519466.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6392..7414
                     /gene="pldB"
                     /locus_tag="RPM73_001084"
     CDS             6392..7414
                     /gene="pldB"
                     /locus_tag="RPM73_001084"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDX6519467.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7422..8222
                     /gene="yigL"
                     /locus_tag="RPM73_001085"
     CDS             7422..8222
                     /gene="yigL"
                     /locus_tag="RPM73_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDX6519468.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8298..9197
                     /gene="bioP"
                     /locus_tag="RPM73_001086"
     CDS             8298..9197
                     /gene="bioP"
                     /locus_tag="RPM73_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDX6519469.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9085..10038)
                     /gene="metR"
                     /locus_tag="RPM73_001087"
     CDS             complement(9085..10038)
                     /gene="metR"
                     /locus_tag="RPM73_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDX6519470.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10156..12417
                     /gene="metE"
                     /locus_tag="RPM73_001088"
     CDS             10156..12417
                     /gene="metE"
                     /locus_tag="RPM73_001088"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDX6519471.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12456..13271)
                     /locus_tag="RPM73_001089"
     CDS             complement(12456..13271)
                     /locus_tag="RPM73_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDX6519472.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13533..14294
                     /gene="udp"
                     /locus_tag="RPM73_001090"
     CDS             13533..14294
                     /gene="udp"
                     /locus_tag="RPM73_001090"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDX6519473.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14435..15862
                     /gene="rmuC"
                     /locus_tag="RPM73_001091"
     CDS             14435..15862
                     /gene="rmuC"
                     /locus_tag="RPM73_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDX6519474.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15957..16712
                     /gene="ubiE"
                     /locus_tag="RPM73_001092"
     CDS             15957..16712
                     /gene="ubiE"
                     /locus_tag="RPM73_001092"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDX6519475.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16726..17331
                     /gene="ubiJ"
                     /locus_tag="RPM73_001093"
     CDS             16726..17331
                     /gene="ubiJ"
                     /locus_tag="RPM73_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDX6519476.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17328..18968
                     /gene="ubiB"
                     /locus_tag="RPM73_001094"
     CDS             17328..18968
                     /gene="ubiB"
                     /locus_tag="RPM73_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDX6519477.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19047..19316
                     /gene="tatA"
                     /locus_tag="RPM73_001095"
     CDS             19047..19316
                     /gene="tatA"
                     /locus_tag="RPM73_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDX6519478.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19320..19835
                     /gene="tatB"
                     /locus_tag="RPM73_001096"
     CDS             19320..19835
                     /gene="tatB"
                     /locus_tag="RPM73_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDX6519479.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19838..20614
                     /gene="tatC"
                     /locus_tag="RPM73_001097"
     CDS             19838..20614
                     /gene="tatC"
                     /locus_tag="RPM73_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDX6519480.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20656..21438
                     /gene="tatD"
                     /locus_tag="RPM73_001098"
     CDS             20656..21438
                     /gene="tatD"
                     /locus_tag="RPM73_001098"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDX6519481.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21435..21923)
                     /gene="rfaH"
                     /locus_tag="RPM73_001099"
     CDS             complement(21435..21923)
                     /gene="rfaH"
                     /locus_tag="RPM73_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDX6519482.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22090..23583
                     /gene="ubiD"
                     /locus_tag="RPM73_001100"
     CDS             22090..23583
                     /gene="ubiD"
                     /locus_tag="RPM73_001100"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDX6519483.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23629..24330
                     /gene="fre"
                     /locus_tag="RPM73_001101"
     CDS             23629..24330
                     /gene="fre"
                     /locus_tag="RPM73_001101"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDX6519484.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24522..25685)
                     /gene="fadA"
                     /locus_tag="RPM73_001102"
     CDS             complement(24522..25685)
                     /gene="fadA"
                     /locus_tag="RPM73_001102"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDX6519485.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25695..27884)
                     /gene="fadB"
                     /locus_tag="RPM73_001103"
     CDS             complement(25695..27884)
                     /gene="fadB"
                     /locus_tag="RPM73_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDX6519486.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28074..29405
                     /gene="pepQ"
                     /locus_tag="RPM73_001104"
     CDS             28074..29405
                     /gene="pepQ"
                     /locus_tag="RPM73_001104"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDX6519487.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29405..30019
                     /locus_tag="RPM73_001105"
     CDS             29405..30019
                     /locus_tag="RPM73_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDX6519488.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30058..31509
                     /gene="trkH"
                     /locus_tag="RPM73_001106"
     CDS             30058..31509
                     /gene="trkH"
                     /locus_tag="RPM73_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDX6519489.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31521..32066
                     /gene="hemG"
                     /locus_tag="RPM73_001107"
     CDS             31521..32066
                     /gene="hemG"
                     /locus_tag="RPM73_001107"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDX6519490.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7468 a         8129 c         8763 g         7739 t
ORIGIN      
        1 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
       61 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
      121 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
      181 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
      241 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgac
      301 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
      361 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtac ttcctgtaac
      421 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
      481 aggctcatta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
      541 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
      601 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      661 tactggaaaa agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac
      721 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      781 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      841 agcagccaca tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc
      901 accaggccgt agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca
      961 aaagtccaca gctggaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     1021 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     1081 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     1141 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     1201 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     1261 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     1321 aagtacgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     1381 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     1441 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     1501 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1561 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1621 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1681 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     1741 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     1801 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1861 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1921 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1981 ctactttagg aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa
     2041 aaacattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     2101 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     2161 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     2221 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     2281 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     2341 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     2401 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     2461 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     2521 ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     2581 gcgttcagac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2641 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2701 ggatatcacc aaatatatgg gttactacca gcttgaaatc ggctatcacc tcggtgatgc
     2761 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2821 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2881 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa
     2941 cgatttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta
     3001 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     3061 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     3121 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     3181 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     3241 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     3301 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     3361 ggcgtgcctt aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg
     3421 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     3481 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     3541 ccaatggggc cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3601 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3661 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3721 tattcgctac atgctgatgg agaagttcaa accgctcgat cagttgatgc gctacgtgca
     3781 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3841 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3901 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     3961 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     4021 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     4081 gcccgcggaa gcgatgctgt tttacgatcc ggccgatatg gcgtggctgc gccgttgtct
     4141 ggaagagaag ccgcaggggc agttgcagga tatcgaacgt cataaactca atgcgatggg
     4201 cgcttttgcc gaagcgcaaa cttgccgtcg tctggtactg ctgaactatt ttggcgaagg
     4261 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     4321 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc gttttggtat
     4381 gggctatgtg gtggaagtga ttcgcggcgc taataaccag cgtatccgcg actatggtca
     4441 tgacaaacta aaagtctacg gtatgggccg tgataaaagt catgaacatt gggtgagcgt
     4501 gatccgccag ctgattcacc tcggcctcgt gacgcaaaat attgcccagc attctgccct
     4561 ccaactgaca gaggctgcgc gtccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4621 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4681 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagcaatgt
     4741 cccgccgtac gtggtgttta acgacgcaac gttgattgag atggctgaac agatgccgat
     4801 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4861 taaaccgttt atggcgctta tccgcgcgca tgttgacggc gacgacgaag agtagtcagc
     4921 ggcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccacgag tcaaatgtat
     4981 gttgatgtta tttctcaccg tcgccatggt gcatattgtg gcgcttatga gccccggtcc
     5041 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     5101 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     5161 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta
     5221 tctctgctgg atgggttacc agatgctacg cggtgcactg aaaaaagagg cggtttctgc
     5281 acctgcgcca caggtcgagc tggcgaaaag tgggcgcagt ttcctgaaag gtttactgac
     5341 caatctcgct aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg
     5401 tgataacgtt ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacgct
     5461 ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca
     5521 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5581 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5641 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5701 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5761 agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata
     5821 gtggtgacgc cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc
     5881 ggaaatagcg ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5941 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaaggtc aattgcacca
     6001 gcggcgcgcc actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac
     6061 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     6121 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     6181 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     6241 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     6301 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg
     6361 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     6421 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     6481 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6541 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6601 tgcggaactg gcctacgatt tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6661 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6721 taatgattat gttgatgatc tggcggcatt ctggcaacag gaggtccagc ccggtccgtg
     6781 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6841 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6901 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6961 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     7021 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     7081 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     7141 ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt ctcttacaag ctgaagagga
     7201 acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     7261 tcctgtcgaa ggaggacgac cgttggtaat taaaggtgct taccatgaga tcctttttga
     7321 aaaggacgca atgcgctcag tcgcgctcca cgccattgtt gattttttca acaggcataa
     7381 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     7441 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     7501 ccctgaagct gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7561 ttgatgtggg gcaaattcgc gataatctgg agatcaagtc gtacatgatc acctccaacg
     7621 gcgcgcgcgt tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca
     7681 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7741 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7801 cggtgttcaa atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag
     7861 tgttcttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7921 gctggggcga tcgcgtcaac gttagtttct ctaccttaac ctgcctggaa gtgatggctg
     7981 gcggcgtttc aaaaggccat gccctggaag cggtagcgaa gaaactgggc tacagcctgc
     8041 aagattgtat tgcctttggc gatggaatga acgatgccga aatgctgtcg atggcgggta
     8101 aaggctgcat tatgggcagc gcgcatcagc gcctgaaaga tctgcatccc gagctggaag
     8161 tgattggtac taacgccgaa gacgcagtgc cgcattatct gcgtaaactc tatttatcgt
     8221 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     8281 tcaaagagtt ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct
     8341 ttagctttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     8401 gcgttggcct ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga
     8461 aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct tggcgtgatg tatatgctga
     8521 gtttccgcgc ttatctctat ctgacggttt ccgaactgct gctgttcacc gtactgacgc
     8581 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggatatg
     8641 cttttagtgc cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8701 accatttctg gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca
     8761 tggtgggtta caaacgcctg atggaaactc gcccgatgcc gcagcataac gcctttgcgt
     8821 ggttctatct tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8881 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8941 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     9001 gaattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     9061 aacagccgca ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg
     9121 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     9181 gcgcgctgag cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc
     9241 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     9301 ctcaaaactc tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat
     9361 ctgaatcaac aataaggtgt tatcgacgct ttttagtgac ggactgacgc ctgccggctg
     9421 aagaaaatgc cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc
     9481 gctggcgaga tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa
     9541 caccagacgc acttcatagt cgaacatcgg cgaataatgc agaccactgc gcggcagaat
     9601 atcggacgtc attaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9661 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat tttctaacgc
     9721 gggcgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9781 cggttcattg caggcttgca gtgcctggct aatttgcggc agcacctggt ttgccagttg
     9841 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg
     9901 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9961 caacgtcgcc gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag
    10021 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
    10081 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaata ctaaaaattc
    10141 cataaggggc atataatgac aattcttaat cacaccctcg gtttccctcg cgttggcctg
    10201 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
    10261 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
    10321 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgttctgac caccagtctg
    10381 ctgctgggta acgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
    10441 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    10501 accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa aggccaacag
    10561 ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc gctgggccac
    10621 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    10681 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaaatgctg
    10741 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    10801 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggccag
    10861 gtgaaactgc tgctgaccac ttattttgaa ggcgtaacgc caaacctcga cacgattact
    10921 gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagatga cgttgttgaa
    10981 ctgcacaagc gcctgccttc tgactggttg ctgtctgcgg gtctgatcaa tggtcgtaac
    11041 gtctggcgtg ccgatcttag cgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    11101 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    11161 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    11221 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    11281 gccccgattc aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt
    11341 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa
    11401 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    11461 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac
    11521 aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg
    11581 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    11641 gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    11701 tgcgtgaagc caccgattgt cattggtgat attagccgcc cggcaccgat taccgttgag
    11761 tgggcgaagt atgcgcaatc tctgactgat aaaccggtga aagggatgct gactggcccg
    11821 gtgaccattc tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    11881 caaattgcgc tggcgctgcg tgatgaagtg gcggacctgg aagccgcagg aattggtatc
    11941 atccagattg atgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat
    12001 gcatatctcc agtggggcgt ggaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    12061 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    12121 gcactggatg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    12181 tcgtttgaag agtttgatta tccgaatgaa atcggtccag gcgtctatga cattcactcg
    12241 ccaaacgtac cgagcgtaga atggattgaa gccttgctga agaaagcggc aaaacgcatt
    12301 ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggccagaa
    12361 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg ggggtaaatc
    12421 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    12481 tgcttaaacc actccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    12541 gggcgataat cggcgttgaa tgcatgcccg gcatccgggt agacgataat ctctgctttc
    12601 gcattagcag cccgcagcgc ctggcgcatg gtttcaacgc tctcctgcgg aatgctgtta
    12661 tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    12721 tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc cactgcagct
    12781 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    12841 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    12901 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    12961 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    13021 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    13081 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    13141 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    13201 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    13261 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    13321 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    13381 agagtgattt atgtgatttg aatcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    13441 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    13501 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    13561 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    13621 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    13681 tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    13741 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    13801 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    13861 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    13921 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatcc attggcgcga
    13981 caactcacgt tggcgtgaca gcttcttctg ataccttcta cccaggccag gaacgttacg
    14041 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    14101 tgggcgtaat gaactatgaa atggaatctg caaccctgct gaccatgtgt gcaagtcagg
    14161 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    14221 atgctgagac gatgaaacaa actgaaagcc aagcggtgaa aatcgtggtg gaagcggcgc
    14281 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    14341 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    14401 tttgtgcggg taagttgttg cgtcaggagg cgtggtggat ttctcaatca tggtttacgc
    14461 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aacatgcgca
    14521 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    14581 acaacaaatt acccaaagcg agcactggcg tgcagagtgc gagttactca ataacgaagt
    14641 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    14701 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    14761 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    14821 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    14881 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    14941 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    15001 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    15061 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    15121 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    15181 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    15241 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    15301 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    15361 ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg accgccagcc
    15421 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    15481 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gcaacgccca
    15541 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    15601 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    15661 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    15721 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    15781 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    15841 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    15901 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    15961 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    16021 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    16081 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    16141 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    16201 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    16261 aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    16321 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    16381 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    16441 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    16501 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    16561 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    16621 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcagggg
    16681 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    16741 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    16801 aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    16861 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    16921 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    16981 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    17041 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    17101 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    17161 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    17221 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    17281 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    17341 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    17401 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    17461 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    17521 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    17581 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    17641 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    17701 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    17761 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gtcattaaag
    17821 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    17881 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt gatgaactga
    17941 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    18001 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    18061 gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc actaacatga
    18121 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    18181 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    18241 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    18301 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    18361 tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    18421 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    18481 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    18541 tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg cagctcgatt
    18601 tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    18661 cgctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    18721 ctgaattggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    18781 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    18841 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gagtgggggc
    18901 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    18961 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa
    19021 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    19081 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    19141 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    19201 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    19261 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    19321 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    19381 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    19441 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    19501 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt
    19561 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    19621 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    19681 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    19741 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    19801 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    19861 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    19921 cgtgatattc ctgtgtctgg tctatttcgc caatgatatc tatcacctgg tatccgcgcc
    19981 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    20041 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    20101 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    20161 gccgctgctg gtttccagct ctttgctgtt ttatatcggt atggcattcg cctactttgt
    20221 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    20281 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    20341 ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct cgccagaaga
    20401 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    20461 gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc tgtttgaaat
    20521 cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    20581 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    20641 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    20701 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    20761 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    20821 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    20881 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    20941 tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc tcagctacgc
    21001 attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    21061 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    21121 ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg cattaccggt
    21181 tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt gattccggcg
    21241 gagaaattgc tgatcgaaac tgatgcgccg tatctgctcc cgcgcgatct cacgccaaag
    21301 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    21361 tggcgtggag aagatgccgc atggctggct gccaccacgg atgctaatgt caaaacactg
    21421 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    21481 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    21541 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataaggggtt
    21601 gccggatcga caatgtcttt cggtttatat accgatagct gatgaatcac cgccgatggg
    21661 actatcgctg gcgacgcgcc aaagcgcacg aagtggctga caccgcgggt cgcgttgata
    21721 gtcgtggtat gaatcacttc tggatcaaat tcgacaaaca ggtagttggg aaacaatggc
    21781 tcactgactg cagtacgttt tccacgcacg attttttcca gggtgatcat cggtgccagg
    21841 caattcacag cctgtctttc gaggtgttcc tgggcacgtt gaagttgccc gcgcttacag
    21901 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    21961 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    22021 gctaatttaa caaatttaca gcatcgcaaa gatgaacgcc gtataatggg cgtagattaa
    22081 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcttgac gctgcttgaa
    22141 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    22201 attgctgacc gcactttgcg tgccggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    22261 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    22321 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    22381 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaagta
    22441 ttgaacatgc cgacaaagcg gctgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    22501 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    22561 ctgattacct gggggctgac agtgacgcgc ggcccgcata aagagcggca gaatctgggc
    22621 atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    22681 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg tttcccggtt
    22741 tctgtggcgc tgggtgccga tcccgccacg attctcggtg cagtcacccc cgttccggat
    22801 acactttcag agtatgcgtt tgccggattg ctgcgcggta ccaagaccga agtggtgaag
    22861 tgtatctcca atgaccttga agtgcccgcc agtgcggaga ttgtgctgga agggtatatc
    22921 gaacaaggtg aaacggcgcc ggaagggccg tatggcgacc acaccggtta ctataacgaa
    22981 gtcgatagtt tcccggtatt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    23041 cactccacct atactggtcg tccgccggat gaacccgcgg tgctgggtgt cgcactgaac
    23101 gaagtgtttg tgccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    23161 ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta cgccggacac
    23221 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaaattt
    23281 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    23341 accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    23401 ctggattttg cctcgcctgt ctccgggctg ggttcaaaaa tggggctgga tgccacgaat
    23461 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    23521 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    23581 tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    23641 agctgtaaag tgacctcggt agaagctatc acggataccg tatatcgtgt ccgcatcgtg
    23701 ccagatgcgg ccttttcttt tcgcgctggt cagtatttga tggtagtgat ggatgagcgc
    23761 gacaaacgtc cgttctcaat ggcttcgacg ccggatgaaa aagggtttat cgagctgcat
    23821 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    23881 catcaaatcg tggtcgatat tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    23941 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    24001 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    24061 catctttatg atctctccga gcttgaggcg ctttcgttaa agcaccctgg tctgcaagtg
    24121 gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac cgtgttaacg
    24181 gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc cggacgtttt
    24241 gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc
    24301 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    24361 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatc
    24421 ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgcaggatc
    24481 ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc
    24541 gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg
    24601 cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacagcc cagcggatga
    24661 cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc cattagcccg
    24721 agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg
    24781 atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg
    24841 taacccataa tcgacggatc acaaccaacg accgccatcg aacgcacgcg ggcgcgcggc
    24901 ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg
    24961 gaaagcgcag aagatgtacc cgccgttacc gtaccgctta ctggatcaaa cgccggacgc
    25021 agcgtggcga gggcttccac ggtggtttcc ggtcgaatca cctcgtcgta attaaactgc
    25081 ttcaggacgc cgtcggcatc gtgaccaccg gtcgggatga tttcattttt aaatgcggcc
    25141 gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca
    25201 cggctgatac cgtgcatacg cgccagcatt tctgctgtta agcccatcat gcccgccgct
    25261 ttggcgacat tgcggctcag gccggggtga aaatcaacgc cgtgactcat cggcacatgg
    25321 cccatatgct ccacgccgcc aaccagacat gcctgcgcat cgccggtcat aatcatccgt
    25381 gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg
    25441 acagagtgtg gtacttctgc cagcagcgcc gcgttacggg cgatgttaaa accctgctcc
    25501 agcgtctgct gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg
    25561 ttacgcgcca gcaggctacg cattaaatga gcggagagat cttctgcacg cacgttacga
    25621 aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt
    25681 tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccggg
    25741 ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc
    25801 gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc
    25861 agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac cagcgccata
    25921 tccgcttccg ccggagtggc gataatgcct tcctccagac agcgcaccac ttcgttgacc
    25981 atcgggatca tcatgcgggc gataatctcc tcttcgctga aatcgcgctt cggctggctc
    26041 acttctgcca gcaggtcgtc aacgacggcg tcttcttctt tcttcggctt acctttgctg
    26101 tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac
    26161 agcgcgtcga tggtatcgcg ataatctttc tgcatccgct gcgggaagcc tgctgccatg
    26221 acagcctggg catgatgcgc ggtatcaatg cccacgacat ccagcagata tgccggaccc
    26281 ataggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg
    26341 cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac aaagaagccg
    26401 gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc gacaactttc
    26461 gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg catccggtgt
    26521 accgggttaa aaaagtgcat tccgcagaag ttttccgggc gttccagcgc gttggccagt
    26581 tcgctgatag gaatggttga agtgttagac gccagcacgg tatccgggcg tactttttgt
    26641 tcggtttctg ccagtacggc ttttttcact ttcgggtttt cgacaaccgc ttctaccaca
    26701 acatccacgc ggtcaaaccc ggcgtagtcg agcgttgggt ggattgtgga gatcacgcca
    26761 gccagtttca gaccatcgat cttgccgcgc tcaagctgct tgttcagcag tttcgcggct
    26821 tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggcacgcct
    26881 ttccacgcag actggtaagc gatgccgccg cccataatgc ctgcacccag caccgcggcc
    26941 tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg
    27001 ttaaggaaaa tgccgaccag tgcgcgggct tcgttggtat gcgccagcgg gacaaaactt
    27061 ttgttttcca ggtttaaggc ttcttcacga ccaaaacggg ccgcagcttc aatggttttt
    27121 actgcggtga tgggggccgg ataatgtttc cccgctgttt gtgcgaccat ccctttagcg
    27181 atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gtggttccag cttcggctga
    27241 cgttttgctt tccagtcgag gtcgccgtta atggcctggc gtaaaaccgc ctttgcgcct
    27301 tcaaccagtt tttctgcttt gactacgcca tccaccagac cgattttcag cgcctgatcc
    27361 gcgccgacat ctttaccggc ggcaatgatt tccagcgcac tgtcagcgcc cagcatacgt
    27421 ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg
    27481 atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccaccg
    27541 cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta
    27601 aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacagg
    27661 gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc
    27721 agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg
    27781 gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca
    27841 tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc
    27901 cgcaaggtga tctggtcgta ccagatgagt cgaagtgtgt attttgtgtt aaaaatatgc
    27961 aaataaaaga ttaaagaaat gccaatctga tcacgctcgg cagaaatcac gctctggatg
    28021 aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat
    28081 cactggcctc actctataaa aatcatatag ctaccttaca ggaacggact cgcgatgcgc
    28141 tggcgcgctt caagctggat gcgttactta ttcactccgg cgaactgttc aacgtttttc
    28201 tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa
    28261 ctcaggtgcc aaactgctgg ctgctggtgg atggcgtgaa caagccgaaa ctgtggttct
    28321 atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctctttc tggactgaag
    28381 acgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg cctgctgcgc
    28441 gcgggaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg ggtattgagg
    28501 ccagcaatat caacccgaaa ggggtgatcg actacctgca ttactaccgc tccttcaaaa
    28561 ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgcg
    28621 cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaacatt gcctatctga
    28681 ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg ctcaacgaac
    28741 acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag atgcgcagct
    28801 tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc cgtacctggt
    28861 cggcaaaaag tgacaacgac tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg
    28921 cgctgatcgc taccatgaaa gcaggcgtca gctatgtgga ttaccacatc cagttccatc
    28981 agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga
    29041 tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat ccgctgggcc
    29101 tgcaggtgca tgacgtagcc ggttttatgc aggatgatag cggtacgcac ctcgcggcac
    29161 cggcaaaata tccgtacctg cgctgcaccc gtattctcca gccgggcatg gtgttaacca
    29221 tcgaaccggg tatctacttc attgaatcgc tgctggcacc gtggcgtgaa gggcagttca
    29281 gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg
    29341 aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat ctgaaactgg
    29401 cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag agatcaaaaa
    29461 gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt
    29521 tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg
    29581 tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg cgggaacggc
    29641 aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta ccgctgtggt
    29701 agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag cgtatggcgg
    29761 cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata
    29821 tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg ggcagtgtga
    29881 cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc
    29941 ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt
    30001 gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg
    30061 cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg
    30121 attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag
    30181 accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg caaagagaaa
    30241 ggcgaactta aatcccgtga ggggtttctg atagtggtgc tgttctggac cgtgctgggt
    30301 agcgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg
    30361 ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt ggggctggat
    30421 tcgctccctc acgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg
    30481 atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat
    30541 cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg
    30601 gcgaaaaccc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg
    30661 tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc
    30721 ggcttctcga cacatgatgc cagtatcggt tatttcgata gcccgactat taacactatc
    30781 attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc actgttaagt
    30841 gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag
    30901 tttacgctgg tggttatttg taccctcgta ctgtggtttc ataatgtcta cagttcggcg
    30961 ctgatgacaa ttaaccaggc gtttttccag gtggtgtcga tggcgacaac cgccgggttt
    31021 acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt atgttcggct
    31081 tttatcggcg gttgtgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg
    31141 ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc
    31201 attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg gggatttttc
    31261 tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat
    31321 gacttttctg cctttgcctc agttgttgcg acattgaata acctggggcc agggcttggc
    31381 gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac
    31441 atgctgtttg gtcgtctcga ggtctttaca ttgctggtgc tctttacccc gactttctgg
    31501 cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa
    31561 cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc cagacggatg
    31621 tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ttatgaccgt gtggtcattg
    31681 gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc aaaaaacatg
    31741 cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg gcgcgcaaac
    31801 cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg aactcgcaat
    31861 ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt
    31921 acgatcgttt tatgatcaag ctaattatga agatgtcagg cggtgaaacg gatacgcgca
    31981 aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccatt
    32041 taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg tcagaaaat
//
LOCUS       DAPPNJ010000030        31881 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000030 DAPPNJ010000000
VERSION     DAPPNJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.030"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            107..550
                     /gene="cmtB"
                     /locus_tag="RPM73_001108"
     CDS             107..550
                     /gene="cmtB"
                     /locus_tag="RPM73_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDX6519491.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQDVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            578..1966
                     /gene="cmtA"
                     /locus_tag="RPM73_001109"
     CDS             578..1966
                     /gene="cmtA"
                     /locus_tag="RPM73_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDX6519492.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1981..3258
                     /locus_tag="RPM73_001110"
     CDS             1981..3258
                     /locus_tag="RPM73_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDX6519493.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3255..4220
                     /gene="yggF"
                     /locus_tag="RPM73_001111"
     CDS             3255..4220
                     /gene="yggF"
                     /locus_tag="RPM73_001111"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDX6519494.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4242..4751
                     /gene="fumE"
                     /locus_tag="RPM73_001112"
     CDS             4242..4751
                     /gene="fumE"
                     /locus_tag="RPM73_001112"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDX6519495.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4748..5461
                     /locus_tag="RPM73_001113"
     CDS             4748..5461
                     /locus_tag="RPM73_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDX6519496.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5433..6110)
                     /locus_tag="RPM73_001114"
     CDS             complement(5433..6110)
                     /locus_tag="RPM73_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDX6519497.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6104..6781)
                     /locus_tag="RPM73_001115"
     CDS             complement(6104..6781)
                     /locus_tag="RPM73_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDX6519498.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLKN
                     GALQPLC"
     gene            complement(6769..7476)
                     /locus_tag="RPM73_001116"
     CDS             complement(6769..7476)
                     /locus_tag="RPM73_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDX6519499.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7477..8055)
                     /locus_tag="RPM73_001117"
     CDS             complement(7477..8055)
                     /locus_tag="RPM73_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDX6519500.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8078..8509)
                     /locus_tag="RPM73_001118"
     CDS             complement(8078..8509)
                     /locus_tag="RPM73_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX6519501.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8881..9900
                     /gene="epd"
                     /locus_tag="RPM73_001119"
     CDS             8881..9900
                     /gene="epd"
                     /locus_tag="RPM73_001119"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDX6519502.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9950..11113
                     /gene="pgk"
                     /locus_tag="RPM73_001120"
     CDS             9950..11113
                     /gene="pgk"
                     /locus_tag="RPM73_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDX6519503.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11328..12407
                     /gene="fbaA"
                     /locus_tag="RPM73_001121"
     CDS             11328..12407
                     /gene="fbaA"
                     /locus_tag="RPM73_001121"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDX6519504.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12765..13625
                     /gene="mscS"
                     /locus_tag="RPM73_001122"
     CDS             12765..13625
                     /gene="mscS"
                     /locus_tag="RPM73_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDX6519505.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13764..14399
                     /gene="argO"
                     /locus_tag="RPM73_001123"
     CDS             13764..14399
                     /gene="argO"
                     /locus_tag="RPM73_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDX6519506.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14492..15232
                     /locus_tag="RPM73_001124"
     CDS             14492..15232
                     /locus_tag="RPM73_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDX6519507.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15399..16295
                     /locus_tag="RPM73_001125"
     CDS             15399..16295
                     /locus_tag="RPM73_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6519508.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16292..17770)
                     /gene="scpC"
                     /locus_tag="RPM73_001126"
     CDS             complement(16292..17770)
                     /gene="scpC"
                     /locus_tag="RPM73_001126"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDX6519509.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17794..18579)
                     /gene="scpB"
                     /locus_tag="RPM73_001127"
     CDS             complement(17794..18579)
                     /gene="scpB"
                     /locus_tag="RPM73_001127"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDX6519510.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18590..19585)
                     /gene="meaB"
                     /locus_tag="RPM73_001128"
     CDS             complement(18590..19585)
                     /gene="meaB"
                     /locus_tag="RPM73_001128"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDX6519511.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPSHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19578..21722)
                     /gene="scpA"
                     /locus_tag="RPM73_001129"
     CDS             complement(19578..21722)
                     /gene="scpA"
                     /locus_tag="RPM73_001129"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDX6519512.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLESVRDVLNLISQHHD"
     gene            complement(21926..22819)
                     /gene="argP"
                     /locus_tag="RPM73_001130"
     CDS             complement(21926..22819)
                     /gene="argP"
                     /locus_tag="RPM73_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDX6519513.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22961..23146
                     /locus_tag="RPM73_001131"
     CDS             22961..23146
                     /locus_tag="RPM73_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519514.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23202..23861
                     /gene="rpiA"
                     /locus_tag="RPM73_001132"
     CDS             23202..23861
                     /gene="rpiA"
                     /locus_tag="RPM73_001132"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDX6519515.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24117..25349
                     /gene="serA"
                     /locus_tag="RPM73_001133"
     CDS             24117..25349
                     /gene="serA"
                     /locus_tag="RPM73_001133"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDX6519516.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25578..25637
                     /gene="ibsC"
                     /locus_tag="RPM73_001134"
     CDS             25578..25637
                     /gene="ibsC"
                     /locus_tag="RPM73_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDX6519517.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25970..26029
                     /locus_tag="RPM73_001135"
     CDS             25970..26029
                     /locus_tag="RPM73_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX6519518.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26130..26732)
                     /locus_tag="RPM73_001136"
     CDS             complement(26130..26732)
                     /locus_tag="RPM73_001136"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDX6519519.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26753..26936)
                     /gene="ssrS"
                     /locus_tag="RPM73_001137"
     ncRNA           complement(26753..26936)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RPM73_001137"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26978..27307)
                     /gene="zapA"
                     /locus_tag="RPM73_001138"
     CDS             complement(26978..27307)
                     /gene="zapA"
                     /locus_tag="RPM73_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDX6519520.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27475..28053
                     /gene="ygfB"
                     /locus_tag="RPM73_001139"
     CDS             27475..28053
                     /gene="ygfB"
                     /locus_tag="RPM73_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDX6519521.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28079..29404
                     /gene="pepP"
                     /locus_tag="RPM73_001140"
     CDS             28079..29404
                     /gene="pepP"
                     /locus_tag="RPM73_001140"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDX6519522.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVIGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29401..30579
                     /gene="ubiH"
                     /locus_tag="RPM73_001141"
                     /gene_synonym="visB"
     CDS             29401..30579
                     /gene="ubiH"
                     /locus_tag="RPM73_001141"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDX6519523.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30602..31804
                     /gene="ubiI"
                     /locus_tag="RPM73_001142"
                     /gene_synonym="visC"
     CDS             30602..31804
                     /gene="ubiI"
                     /locus_tag="RPM73_001142"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDX6519524.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASFYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7624 a         7912 c         8598 g         7747 t
ORIGIN      
        1 ctgttttcaa aacatcggtt tccctgctca attgctttca ttaaaccgca cgagtatttt
       61 atttttaccg cctgacggta ccctaaaaat aaaataagga taatttatgc ggcttagtga
      121 ttattttccg gaatcatcaa tctcagttat acattcagca aaagattggc aggaagctat
      181 cgatttctcg atggtatcat tgctggataa aaactatatc agcgagaatt acattcaggc
      241 aattaaagat tccaccatta acaatggccc ttattatatt ctcgcaccag gcgtggcaat
      301 gcctcatgcg cgaccggaat gtggggcgct taaaaccggg atgtctttga cattacttga
      361 acaagatgtt tattttccgg ggaatgacga accaattaaa ttactcatcg gactctctgc
      421 tgccgatgcc gattcgcaca ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga
      481 agaaatactc gaacaactct taacagcatc atcagaaaaa caattagcgg acattatcag
      541 ccgcggataa ttactctctc ctctacagga taatattatg gaaaacaagt ctgctcgtgc
      601 aaaggtccag gcttttgggg gctttttgac tgcaatggtc atccccaata ttggtgcttt
      661 tattgcctgg ggcttcataa ctgcgttatt tattcccacc ggttggctgc cgaatgaaca
      721 tttcgccaaa attgtcggcc cgatgattac ctatttattg cccgtgatga ttggttctac
      781 tggtggtcat ctggtcggcg gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg
      841 tgtgatcgtt ggcgcagaga tcccgatgtt ccttggctca atgatcatgg ggccgctcgg
      901 tgggttggtc ataaaatatg tcgataaggc actggaaaaa cgcatacctg ccggttttga
      961 gatggttatc aataacttct cattaggtat cgcggggatg ctcctttgtc tgctgggttt
     1021 tgaagtgatc ggcccggcgg tgttaattgc caatactttc gtcaaagagt gtatcgaggc
     1081 gctggtacat gcgggttatc tgcctctgct gtcagtcatc aatgaaccgg cgaaagtgct
     1141 tttccttaat aatgcgatcg atcagggcgt ctattatccg ctgggaatgc aacaggcttc
     1201 ggttaacggt aaatccatct tctttatggt ggcctctaac ccaggtccgg gcctggggct
     1261 gctgctggcg tttaccttgt ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat
     1321 gattattcac ttcctcggtg ggatccacga actgtatttc ccatatgtgc tgatgaagcc
     1381 gctgactatt attgccatga ttgcgggcgg tatgtctggt acctggatgt ttaacttact
     1441 ggacggtggt ctggtggctg gcccaagtcc ggggtctatc tttgcttacc tggcactgac
     1501 gccgaaaggc tcgttcctgg cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt
     1561 tgctattact tcgctgatac tgaagatgga aaaaacggtg gaaacggaga gcgaagatga
     1621 gtttgctcag tcagccaatg cggttaaggc gatgaaacaa gagggtgcat tctcgttaag
     1681 cagggttaag cgtatcgcct ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc
     1741 gaccaccttc cgtaaacgcc tggaaaaagc ggggctggca atagaagtaa aacattacgc
     1801 gatagaaaac gtgcctgcgg atgccgatat cgtcgtcact cacgccagtc tggaagggcg
     1861 cgtgaaacgt gtgacggata aaccgctgat attgattaat aactatattg gcgatccaaa
     1921 actcgacact ttatttaatc aattaaccgc cgaacataaa cactgattac agaggtaaaa
     1981 atgaaaacca aagttgctgc tatttatggc aagcgggatg tccgtctgcg cgaatttgaa
     2041 ctgccagaaa ttaccgataa tgaactgtta gtgagtgtaa tttctgacag cgtctgttta
     2101 tcgacctgga aagcggcgtt actcggtagt gaacataaac gcgtacccga cgatttagaa
     2161 aatcatccgg tcattaccgg gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat
     2221 ctcactggca aatataaaaa aggccagcgt tttgtattgc aaccggcgat ggggttacca
     2281 agcggatatt cagcaggcta cagctacgaa tattttggcg gtaacgccac ctatatgatt
     2341 attcccgaaa tcgccattaa tttgggctgc gtattaccgt atcacggctc ttattttgct
     2401 gcggcgtcgc tggcagagcc tatgtgctgc attattggtg cttatcatgc caattatcac
     2461 accacgcaat atgtttatga gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg
     2521 ctggcgtgtg cgggtccgat gggcattggc gctatcgatt acgccattaa cggtggcata
     2581 caaccgtcac gggtggtggt ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag
     2641 ctgctgccgg tggatctggc ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa
     2701 gggatgagcg atccggtcca gacgctgcgg gcgctgacag gagatgtcgg gttcgatgac
     2761 atttttgttt atgcggcggt gcctgctgtc gttgagatgg ctgacgaatt actggcggaa
     2821 gatggctgtc tgaacttctt tgccgggccg acggataaaa acttcaaagt gccgtttaat
     2881 ttctacaacg tccattacaa cagcacgcac gtagtcggta catccggcgg ttcaacggac
     2941 gacatgaaag aggcgattgc gttaagcgcc acggggcagt tgcagccgtc ctttatggtc
     3001 acgcatatcg gtgggctgga tgcggtgcca gataccgtgc tcaatctgcc ggatatccct
     3061 ggcggtaaaa aactcattta taacggcgtg accatgccgc tcactgccat tgccgatttt
     3121 gccgaaaaag gcaaaaccga tccgctgttt aaagagttgg cgcggctggt tgaggaaacg
     3181 cacggcatct ggaatgaaca ggccgagaaa tatctgctgg cacaatttgg cgttgatatc
     3241 ggggaggccg cgcaatgatg tccctggcgt ggccattatt tcgcgttacg gaacaggcag
     3301 cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa taaaattgat ggcctggcgg
     3361 tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg
     3421 aaggagaaat tgaccatgcg ccgatgctgt ggattggcga agaggtgggc aaaggcgatg
     3481 ggccagaagt cgatatcgcg gttgacccga ttgaaggcac gcggatggta gcgatggggc
     3541 agagcaatgc gctggcggtc atggctttcg cccctcgaga tagcctgtta catgcgcccg
     3601 atatgtatat gaaaaagctg gttgttaatc gactggctgc cggggcgatt gatctgtctt
     3661 tgcctctggc ggacaacctg cgcaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc
     3721 tgcgcatggt tacgctggat aaaccgcgcc tgagtgccgc gattgaagaa gcaacacagc
     3781 tgggcgtgaa ggtttttgcc ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct
     3841 ggcaggataa tccttacgac gtgatgtaca ccatcggcgg tgcgccagaa ggtgtgattt
     3901 ctgcctgtgc cgttaaagca ttaggtgggg atatgcaggc cgagctgatt gacttttgtc
     3961 aggctaaagg ggattacacg gaaaatcggc agattgcaga gcaggagcgc aagcgttgta
     4021 aggcaatggg cgttgacgtt aaccgtgtgt actcgctcga tgaactggtg agggggaacg
     4081 atatcctctt tagcgctacg ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga
     4141 cggcgaatgg ggtgcggacg cagacattac tgatcggcgg cgcggaccaa acgtgtaata
     4201 taatagactc cctgcattga tggtgatcta tcggaagacc aatggcgacg ctgacagaag
     4261 atgatgtgct tgagcaactg gatgcacagg acaatttatt ttcatttatg aaaactgcgc
     4321 attctatttt gctccagggg atacgccagt ttctgccgtc gctgtttgtc gataacgatg
     4381 aagagatcgt cgaatatgca gtgaagccgt tactcgccca aagcggcccg cttgacgata
     4441 ttgatgttgc gctgcgtttg atttatgcgc tggggaaaat ggataaatgg ctgtatgccg
     4501 atatcacgca cttttcccag tactggcatt acctgaacga acaggatgaa acgcccggat
     4561 ttgccgatga catgacctgg gattttatca gcaacgtcaa tagcataacc tgcaatgcga
     4621 cgctctatga cgcgttaaaa gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc
     4681 gttttagcgg aatggtcaaa acggcgctga cgctggcagt aacgaccacc ttaaaggaat
     4741 taacgccgtg aaaattgaat taacggtgaa tgggctgaac gtgcaagcgc agtaccatga
     4801 tgaagagata gaacgcgtgc ataaaccgtt gctgcgtatg ctggcggcct tacagacggt
     4861 aagtccgcag cgacgtacgg tggtttttct ttgcgctccg ccggggacgg gtaaatctac
     4921 cttaactacc ttctgggaat atctcgcgca acaagaccct gaattacccg ctatccagac
     4981 gctcccgatg gatggttttc accattacaa tagctggctg gatgcgcatc aattgcgccc
     5041 cttcaaaggc gcaccagaga catttgacgt tgcgaaactg gcggaaaatc tgtgccaggt
     5101 agtggaaggg gattgtacgt ggccgcagta tgatcgacaa aagcatgatc ctgttgaaga
     5161 tgcgttgcac gttaccgcac cactcgtcat cgtcgaagga aactggttgt tgctggacga
     5221 cgagaagtgg tgccagcttg cgcaattttg tgatttctcg atctttatca acgcgccagc
     5281 tactgcgttg cgggaacgac tggtagggcg caagctggcg ggaggggtat cgctggcaga
     5341 cgcagaagct ttttatgacc gtaccgacgg gccgaatgtt cgccgggtgc tggaagagag
     5401 tctgcctgca aatttgacct taatgatgac cgctacgggt gaatatcgtc tggtggatta
     5461 acgttcctga ttagaccatc ttccagtcgc accacacgag agaaatgacg ccgggtaaat
     5521 gcggcgtcat gactgattgc tacgacgctc gtgcctcgct ggcggcactt ctccagccag
     5581 ctttcaaaca cgctcaacca attttcgtca aaatcccgac tcggctcgtc cagcagcaat
     5641 agtggcggag agagtgcttc caggcaggcg acggcgacca tccgtcgctg cgcactatgc
     5701 agatccaacg ggtgcgcgtt ggcagtgtct gctaactgac aacattgcag ggcagcatgg
     5761 gtacgttgag tgatttcatc cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg
     5821 tcagcgacgg tgctgtgaaa aagttggttt tccgcttcct gaaacagtac gccaaccttt
     5881 gccgcgcgct ggcggttttt caggtttttc atcgcctgtt gttgcagcat aaccgtaccg
     5941 gctgtgggcg taagaagacc tgccattact cgtaacagcg tcgattttcc cgcgccattg
     6001 tcgccggtca gcgccaacca ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa
     6061 tccgttgccg cgccaggcca gcgataagat atcttattta acgttaacat agtggctgga
     6121 gcgctccgtt tttcaattgc cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac
     6181 gttcaaaaag aataatcagc gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca
     6241 gcatttcgct ggcctgcgga gtcaggcggc taaaggcttc atcaagaatt aagagctttg
     6301 gctgcatcgc gatggcgcag gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg
     6361 ccgggtgacg atggcgcaac ggctggcact cggtcagagt cagggcggca tcaatccgcg
     6421 ccataatctc tgcttcagca agacacagat tttctggccc aaaggcaact tcttcttcca
     6481 cgctaaaggt acagccggag agctgcaaat agggcgattg ctggacgagc tggatggtgg
     6541 cggattgttc attaagcggg agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg
     6601 ttatctctcc tggcagaaag tccggatacc agcccgccat cagctgtgcc agcgtacttt
     6661 tgccactgcc gttgtcacca aagatggcga ccatccccgg tttgacatag tgaaagtcat
     6721 aacagaacgg aggatgcgtt gagtgtgtcg ggcaataacg gaactgttct aacgtaacca
     6781 tatccacgct ccgaattctg tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg
     6841 tagggtgcta tcggctggtg cccataacgt tgttcgtcgg ttgtggatcc gaaatgcccg
     6901 catatccagc gccgcaccac ggacagccag atcgttaagc gcattgtgtg ttagcggaat
     6961 aattaacgcg ggcatcgcac gtaaacgctg ataccagccc tcatccagcg gcacgccgcg
     7021 ggcacgctgc gcttcatgaa taatcgctaa ttgccgtttt aactgctcaa cgaccaataa
     7081 cggcccggca aacagataag cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat
     7141 aaaacattgc acggggacgt actgcatcca caactgtgag gttgaaacaa tcgccagaat
     7201 ccgcaaccag agcgtaatag catgcgacca ccgttctggc gagcgtggcg tgccgcttag
     7261 ccattctggt agccagccac cgtgtaccag ccacagcccg gctcccagcg agaacatcag
     7321 ccagacgaca tattttgccc gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt
     7381 cgccgcgcta tagatcggca aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc
     7441 caatgcccac agtgttaatg aggtaaatgg atgcatttag cgcacggcag ccattgccgg
     7501 aaaatggcgc gtggtgcgaa tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt
     7561 aagaatttta tcgaccaggt tcgcgcctat caccgtaatc gccaccgact ccaccaggtt
     7621 ttgccccatc gagtgcatcc aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc
     7681 gaacagcgca gtacgcagcg gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc
     7741 gctgacaacg acttttggca gggtgcgaaa ccaacccgct cgcgccaggc aaccggcgac
     7801 caggccgatg accatcgcga cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc
     7861 ccagagtaaa ttggtcagta agccggtcag cattccgatc accggaccaa gcagtaccgc
     7921 gctaataagt gtgccgatgg agtcgagaaa tataggtaat ttgaccatgc tggccagttg
     7981 cccgccgatc atattgatgg cgatagatat aacgatcaac accagtgcct gactggaaaa
     8041 gtggctacgc gccataagaa aacctctttg aaagtagtta tttaacgggg gaaatatgca
     8101 actcgtcgta accagaaagc gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac
     8161 cgatcaccag ttcaagcgtg gtccgcacgg tacagccggg catcatatga ccgccaagca
     8221 tcgtgccgtg tggatcggaa acgcagagat gaagatgctc accactctgt tccagcgtgc
     8281 cgtttagtga gatcacttcg aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat
     8341 agcgcaaggc gacgtcagtc aggctaccgg tacagcctgc aatccaggcg gcatgaagtt
     8401 gatgctgctg tgcaaaagcg tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca
     8461 gtgcgtaaaa gcgtgcggag gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc
     8521 gaaactcgga cgtattgcgg ataatatcga cgctgcatca gtcatattct gatataagtc
     8581 agggtttgtg acggcacaac gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg
     8641 gaataaattc cacaggggcg gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt
     8701 gactggacaa tcattccttt tattccacgt ttcgcttatc ctagctgaag cgtttcagtc
     8761 gattaaatgt tcgacaatta accaatcagt cgcagtttgc gacaggtaag gtttccccgg
     8821 acgatttgct ggattactct gtctggcaaa tttgctcagg gaaaaccttg caggagatct
     8881 atgaccgtac gcgtagcgat aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct
     8941 ttgtatgaat ccggacgccg ggcggaaatt accgtggtgg caatcaacga actggcggat
     9001 gctgcgggca tggcgcattt gttgaaatat gacaccagcc atggccgttt tgcatgggaa
     9061 gtacgacagg aacgcgatca actttttgtt ggtgatgacg ccatccgcgt attgcatgaa
     9121 cgttcactgc aatcgctccc ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc
     9181 ggcgtatatg gctcccgcga gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg
     9241 ctcttttcac atcctggcag taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag
     9301 gatcaacttc gtgcggaaca ccgcatcgtt tctaacgctt cctgtaccac gaattgcata
     9361 attcccgtca tcaaattgtt agatgatgcg tacggtattg agtccggcac agtgaccaca
     9421 attcactccg ccatgcacga tcaacaggtt attgatgcat accatcctga cctgcgtcgc
     9481 acccgggcag ccagccagtc gatcattccg gtcgatacta aactggccgc cggtatcaca
     9541 cgattttttc cgcaatttaa cgatcgcttt gaagcgattg cggtacgtgt gccaaccata
     9601 aatgtgacgg caatcgattt aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc
     9661 aacctgttgc tgcaaaaagc agcacaaggt gcatttcatg gtatagttga ctatacggaa
     9721 ttgccgttgg tctctgtaga ttttaaccac gatccgcaca gtgccattgt cgatggcacc
     9781 caaacccggg tcagtggcgc acacctgatc aaaacgttgg tctggtgcga taacgaatgg
     9841 ggctttgcta accgaatgct cgacacgacg ttagctatgg ctactgttgc tttcaggtaa
     9901 gacgcaagca gcgtctgcaa aacttttaga atcaacgaga ggattcacca tgtctgtaat
     9961 taagatgacc gatctggatc ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt
    10021 accagtaaaa gacgggaaag taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat
    10081 cgaactggcc ctgaaacaag gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac
    10141 cgaaggcgag tacaacgaag aattctctct gctgccggtt gttaactacc tgaaagacaa
    10201 actgtctaac ccggttcgtc tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg
    10261 tgaactggtt gttctggaaa acgttcgctt caacaaaggc gagaagaaag acgacgaaac
    10321 cctgtccaaa aaatacgctg cactgtgtga cgtgttcgta atggacgcat tcggtactgc
    10381 tcaccgcgcg caggcttcta ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg
    10441 cccgctgctg gcagctgaac tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc
    10501 gatggtggct atcgttggtg gttctaaagt atctaccaaa ctgaccgttc tggactccct
    10561 gtctaaaatc gctgaccagc tgattgttgg tggtggtatc gctaacacct ttatcgcggc
    10621 acaaggccac gatgtgggta aatccctgta cgaagctgac ctggttgacg aagctaaacg
    10681 tctgctgacc acctgcaaca tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc
    10741 tgaaactgca ccggctaccc tgaaatctgt taacgatgtg aaagctgacg agcagatcct
    10801 ggatatcggt gatgcttccg ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat
    10861 tctgtggaac ggtccggttg gcgtgttcga attcccgaac ttccgcaaag gtactgaaat
    10921 cgtggctaac gctatcgcag acagcgaagc gttctccatc gctggcggcg gcgacactct
    10981 ggcagcaatc gacctgttcg gcattgctga caaaatctcc tacatctcca ctggcggcgg
    11041 cgcattcctc gaattcgtgg aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg
    11101 cgctaagaag taaaaaatca cagggcaggg aaacctgccc ttgtttcagc gcgcttttag
    11161 agcacgcatt ttttcatact ctaaataatt cgagttgcag gaaggcgaca agagagtgaa
    11221 tccccaggag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    11281 acttgaagta tgacgagtat aaggcccgac gatacaggac aagagacatg tctaagattt
    11341 ttgatttcgt aaaacctggc gtaatcactg gtgatgacgt acagaaagtt ttccaggtag
    11401 caaaagaaaa caacttcgca ctgccagcag taaactgcgt cggtactgac tccatcaacg
    11461 ccgtactgga aaccgctgct aaagttaaag cgccggttat cgttcagttc tccaacggtg
    11521 gtgcttcctt tatcgctggt aaaggcgtga aatctgacgt tccgcagggt gctgctatcc
    11581 tgggcgcgat ctctggtgcg catcacgttc accagatggc tgaacattat ggtgttccgg
    11641 ttatcctgca caccgaccac tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg
    11701 acgcgggtga aaaacacttc gctgctaccg gcaagccgct gttctcttct cacatgatcg
    11761 acctgtctga agaatctctg caagagaaca tcgaaatctg ctctaaatac ctggagcgta
    11821 tgtccaaaat cggcatgact ctggaaatcg aactgggttg caccggtggt gaagaagacg
    11881 gcgtggacaa cagccacatg gacgcttctg cactgtacac ccagccggaa gacgttgatt
    11941 acgcatacac cgagctgagc aaaatcagcc cgcgtttcac catcgcagcg tccttcggta
    12001 acgtacacgg tgtttacaag ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt
    12061 ctcaggaata tgtttctaag aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc
    12121 acggtggttc cggttctact gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga
    12181 aaatgaacat cgataccgat acccaatggg caacctggga aggcgttctg aactactaca
    12241 aagcgaacga agcttatctg cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga
    12301 acaagaaata ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc
    12361 gtctggaaaa agcattccag gaactgaacg cgatcgacgt tctgtaagat attcctttct
    12421 gcttatctca aggcccgctc tgcgggtctt tttttcgcca aaagcaaaat tgcctgatgc
    12481 gctacgctta tcaggcctac acgtatcctg caatatattg aatttgcagg tttttgtagg
    12541 ccggataagg cgttcacgcc gcatccggca tgacaaagcg cacattgata tctgtctgag
    12601 ccccgctctg cggggatttt ttcgccaaaa gcaaggtgat tcagatgaga aatctgtgat
    12661 ctatttggca aaattatgct ttattgttta cccttgtcag actgcccgtc ataaggcggc
    12721 ggagtgtatt tctccatttt gagtcagttg aaaaggaata ttgaatggaa gatttgaatg
    12781 ttgtcgatag cataaacggc gcgggaagct ggctggtagc taaccaggcg ctgctgctaa
    12841 gttatgcagt aaacatcgtg gcggcactcg cgatcatcat cgttggtttg attatcgcgc
    12901 ggatgatttc caacgcggtg aatcgcctga tgatctcccg taaaatcgat gccactgttg
    12961 ctgattttct ctctgcatta gtccgttacg gtattatcgc ctttacgcta atcgctgcac
    13021 tgggacgcgt gggtgtacaa accgcgtccg tcattgctgt actcggtgcc gcaggcttag
    13081 ctgttggtct ggctttgcag gggtcactgt ctaacctggc cgctggcgtg ttacttgtca
    13141 tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc ggtactgtgc
    13201 tgagtgtgca gattttctcc accaccatgc gtactgcaga cggtaaaatt atcgttattc
    13261 cgaacggtaa aattattgcc ggaaatatta ttaacttctc ccgcgagcca gttcgccgta
    13321 acgaatttat tattggcgtg gcgtatgatt ccgatatcga tcaggttaag cagatcctga
    13381 ccaatattat ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact gtgcgcctga
    13441 acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt ctggagcaac agcggcgatc
    13501 tgcaaaacgt gtactgggat gtgctggagc gtattaaacg tgaatttgat gccgccggta
    13561 tcagcttccc gtacccgcaa atggatgtga actttaagcg ggtgaaagaa gacaaagctg
    13621 cgtaatcaac gctgaggcca gataatactc tctggcctct ctcttattag ttattctgat
    13681 tgccaattaa tattatcaat ttccgctaat aacaatcccg cgatatagtc tctgcatcag
    13741 atacttaatt cggaatatcc aacgtgtttt cttattactt tcaaggtctt gctcttgggg
    13801 cagctatgat tctgccgctc ggcccacaaa atgcttttgt gatgaatcag ggcattcgtc
    13861 gtcagtatca cattatgatt gccttacttt gcgctatcag cgatttggtc ctgatttgcg
    13921 cggggatttt tggtggtagc gcgttattga tgcagtcgcc gtggttgctg gcgctggtca
    13981 cctggggcgg cgtagtcttc ttgctgtggt atggttttgg cgcttttaaa acagcgatga
    14041 gtagtaatat tgagttagct agtgccgaag tcctgaagca aggcagatgg aaaattatcg
    14101 ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta cctggatact tttgttgtac
    14161 tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg ttggtttgcg ctcgggacaa
    14221 ttagcgcctc tttcctgtgg ttctttgggc tggctattct cgcagcctgg ctggcaccgc
    14281 gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt agtgggatgt gtcatgtggt
    14341 ttatagcctt gcagctggcg agagacggta ttgctcatgc acaagccttg ttcagttagg
    14401 cgctatctga tggaaaaata aaacagaggc gctaagcttg cctccagagg tcctgaattt
    14461 tgggcaagtc aattaacacg gagagactaa cgtgaagttc aaagttatcg ccctggcggc
    14521 attaatgggt attagcggga tggcagcgca ggctaacgaa ttgccggatg gaccgcatat
    14581 tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac attgccactc ttgcgattga
    14641 agttaacgtg gccgcgaagg atgccgctac agccaagaaa caggcagatg agcgcgtcgc
    14701 acaatacatt tccttccttg aactcaatca gatcgcgaaa aaagatatca gctcagcgaa
    14761 tttacgcacc cagccagatt atgattatca ggatggtaaa agtatcctga aaggctaccg
    14821 cgctgtgaga acggtggaag tcacgctccg tcagttagac aaactgaatt ccttgctgga
    14881 tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg tcgctgggcg tggcgcagcc
    14941 ggatgcctat aaagacaaag cgcgtaaggc agcgattgat aacgcgattc atcaggcgca
    15001 ggaactggcg aacggctttc atcgtaaact ggggccggta tatagcgtgc gctaccatgt
    15061 ttccaactat cagcccagcc caatggtgcg gatgatgaaa gccgatgccg cgccggtgtc
    15121 tgcccaggaa acttacgagc aggccgctat tcagtttgat gatcaggtcg atgtggtctt
    15181 ccagttagaa cctgtggatc aacaacccgc taaaacacct gcagcacaat aatacttgta
    15241 gcccgacgta ttcgtcgggc taatgccttt acccgatatt gcctcctttt tctgtaattt
    15301 tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg tttcatcaag gataggctga
    15361 ctgaaacggt taagcggaca aggatgcggg gaggggggat ggacattttt atttcgaaaa
    15421 agatgcgcaa tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa
    15481 tccatatgac ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct
    15541 atacgctatt tacccgcaaa gataataata tcagcctcaa taaagccggt caggaactgt
    15601 atcaaaaact gttcccggtt tatcaacgac tttctgcgat tgataatgaa atccataact
    15661 cagggcgtcg ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt
    15721 tcgatcagtt gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt
    15781 tcagcgaaaa cggtgttatc gataatcttt ttgaccgtca gttagatttt attatctccc
    15841 cacagcatgt atctgcacga gttcaggaac tggaaaacct gactatcagt gaattaccac
    15901 cgctgcgatt gggctttctc gtttctcgcc gttatgaaga gcgtcaagag caagagctgc
    15961 ttcaggagct gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga
    16021 ttgatgcgaa tatgcgacct tgtgggatca atcccaccat tatttatcgt ccctacagtt
    16081 ttatggccaa aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg
    16141 cctggcgact ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt
    16201 atatgcagga atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc
    16261 atattgctga agatcgtgac gggacaaacc attaacccag catcgagccg gttgcaatta
    16321 aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat
    16381 tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatag
    16441 tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt tcggtgatga
    16501 tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga
    16561 tcttgccttc tttagcaatc gacggagcca taaagatcga cagatacgcg ttgcgttcaa
    16621 aatcaccgct gccgccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg
    16681 catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga
    16741 tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat
    16801 ccatattgtc gtaaatcttg cgcagggaat cggccgagat tgtcaggctg gaggcgctgg
    16861 ccccgctgat tttgccggtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat
    16921 acatcatgaa cggaggaatt tccgggtttt cccccagacg cgccattacc gcattattga
    16981 tattgcccac gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt
    17041 cctgcaataa gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat
    17101 ccagcatatt accggcgtcg ggcaagttgg tttccacgac ggcgacaatc tttttcggat
    17161 cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc
    17221 gccgcggtgg cgcgccagga atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat
    17281 agtgattaag ttcaatgatc actttcttcg ctcgcagcag ccaggtcggc gcattaccga
    17341 tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa
    17401 catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt
    17461 caacgaaact caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg
    17521 gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg
    17581 acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc
    17641 tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc cggggtaaag ccgctaaatg
    17701 ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact
    17761 gagtttccat ttttactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg
    17821 tttttcgagg aaagcgttca ttccttcctg gtaatcttcg ctgtcataaa ccgcgcgtcg
    17881 catcccctga atacgttcaa attcatcgga gttcatggtg tgcgcttcgc ccagtacacg
    17941 cagctcttct ttgataacag caatggctaa cggcgctttc tcagagatgt ggtgcgccat
    18001 ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc
    18061 caacgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agttctttga caatgtggaa
    18121 gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac
    18181 aggcgtcatt gagaaggttg aggtactggc ggcgataatc agatcggaac tcatgatcat
    18241 ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt tcgggaattt
    18301 ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat cgcgaccgcc
    18361 agacggcagt tcgtgaatat cgtggcctgc ggagaagact ttggatccac tcggcgcgcg
    18421 caaaatgata cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc
    18481 aataaagact ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac
    18541 tttgttgata gtgacaacgt taacatactg ataagacata aaaattcctt taatcaaaat
    18601 attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt
    18661 ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggt
    18721 gatgcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca
    18781 cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga
    18841 tctcatcgat tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt
    18901 cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat
    18961 tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact tccatcagcc
    19021 cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt
    19081 ccaccatgcg ggcgacttcc gtttccgact gcccgacgcc aaccgtttcg acaatcacta
    19141 cgtcataacc cgccgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc
    19201 ccagatgacc ggaggatggt accgggcgaa taaacgccgc ttcggcacgc gccaggtcat
    19261 tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg
    19321 caataaccgc gacctttaat ccctcacgaa tcaacaacat gccaaaggct tcaagaaagg
    19381 tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta ccgcagtacg
    19441 gcataatggc atcaagcagt tgcgtactta gcgcctgatg actcgggtga cggctttcca
    19501 ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg acgtaagcgg cgaatacttt
    19561 ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcaca
    19621 ctctcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag
    19681 gcgtaatcct gcggtggaat gacgccaccg gcgactacgc agatatcttc gcgtccccat
    19741 tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat
    19801 gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc aggtgtagag
    19861 aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttc
    19921 gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg gcgacgacca
    19981 ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga ggccgatttc
    20041 tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg caccagataa
    20101 cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagcg
    20161 gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc
    20221 aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt
    20281 tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc
    20341 agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg
    20401 cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg
    20461 ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat ttgatcggtc
    20521 agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct
    20581 tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt aggcaaacca
    20641 agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt cgcagccagc
    20701 gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct
    20761 gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc aaatccactg
    20821 accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc
    20881 atgccgatac cgaagaagaa cgacaggcga ggagcgaagt catcaatttt cagtccggca
    20941 gagattgctg ctttgatgta ctcaatccca tcagcgagcg taaatgctac ctgctgcacg
    21001 cagttggcac ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc
    21061 atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg ttttggcggg
    21121 taaatatagg tgttgcggca gaggtactct ttgaggatat cgttctgaat ggtgccggtc
    21181 agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc cagtactggt
    21241 agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac
    21301 aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac gtcacccgcc
    21361 acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc aacggaaaga
    21421 cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa
    21481 aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca
    21541 cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct
    21601 tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca
    21661 gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg ctcgttagac
    21721 attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt
    21781 acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat
    21841 ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat
    21901 gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga
    21961 gcaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga
    22021 gcatccggcg ttgaaatagc cccggcgtta agtcaatcag ttcaccgctg gccagttctt
    22081 tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca agttgtacga
    22141 acgcttctga agaattaacg atatggcagg gcacgctgcc tggaggcaga tcgaagtttt
    22201 gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact ggcgctttca
    22261 gtaatgccga acgcgttacg ccgttaggga aatatttttc ggcaaagggt tttgagctga
    22321 cgaacagata gtcgagcgca ccaagtttat cgacaagaca gctcggcagc gcctgatgtt
    22381 gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat
    22441 cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc agtgcaggaa
    22501 gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc ggagtcgaac
    22561 cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc agcagtgcca
    22621 gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc
    22681 cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct
    22741 tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc
    22801 tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat
    22861 gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca tttgtattcg
    22921 tggttaaacg cgcagcttaa aagatcaggg atttgcgcta atgcatttcg ctcagcgagt
    22981 tcgcgccctg gttgtgttga atggcgtggc gttattgcct ctgttcgcac cgtggagcgg
    23041 catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg ccattgcgcg
    23101 atattttatg gatgagttaa ccacgcggct tgccaacggg gtctgaatcg ctttttttgt
    23161 atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag gatgaattga
    23221 aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggcaccatt gttggtgtag
    23281 gtacaggttc caccgccgca cactttattg acgcgctcgg tacaatgaaa ggccagattg
    23341 aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg
    23401 tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca gatgaaatca
    23461 acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg
    23521 cttcggttgc agaaaaattt atctgtattg cagacgcttc caagcaggtt gatattctgg
    23581 gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg gcgcgtcagc
    23641 tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat aatggcaacg
    23701 tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa aacgccataa
    23761 atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc
    23821 tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg ggaacctccc
    23881 ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt accaggcaat
    23941 tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat tacctttgcg
    24001 tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta ttttttgata
    24061 tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt gggtaaatgg
    24121 caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc gtgcaccaaa
    24181 aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac aaaggcgcgc
    24241 tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc ctgcgatccc
    24301 gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct attggctgtt
    24361 tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg atcccggtat
    24421 ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc gaactgctgc
    24481 tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg tggaacaaac
    24541 tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc tacggtcata
    24601 ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac ttttatgata
    24661 ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct gacctgctga
    24721 atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg
    24781 gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc tcgcgcggta
    24841 ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg gcgggggcgg
    24901 caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc tctccactgt
    24961 gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag gaagcgcagg
    25021 agaatatcgg cctggaagtt gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc
    25081 tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt cgtctgatgc
    25141 acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc gccgagcagg
    25201 gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat gtagttattg
    25261 atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg
    25321 gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa cgggcaggtc
    25381 actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg cattctactt
    25441 tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt tatttaatgg
    25501 ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc gaagcgaggg
    25561 cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg ttactgataa
    25621 gtttcggagc ttattaacag tcagtctcag gggaggagtc atcctccctt acccttactc
    25681 actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg catcgccctg
    25741 aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata gatccttttt
    25801 cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga aaagatttaa
    25861 aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg cagcatgggg
    25921 ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta tgatgcgact
    25981 tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac agtcagtcac
    26041 aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga atcaacgatg
    26101 tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga cggtgtaacc
    26161 accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa ctgacaatca
    26221 tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt ccgatcataa
    26281 aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag cggtgtgatc
    26341 agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg ctcatggatc
    26401 ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag caaattaccg
    26461 gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca gagttgttct
    26521 attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac cgtatgcgcc
    26581 atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat ttcctgctgt
    26641 tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc ttgtcgggat
    26701 aatgtcagtg ggagttctgg tagttgcgtc atggctggtg ccagataaga agggaatctc
    26761 cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat gtgtcgtcgc
    26821 agttttaagg cttctcggac ggaccgagca tgctcaccaa ccgcggagcg ccacattctt
    26881 gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag agaaattttg
    26941 tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt tggttagttt
    27001 tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc agcatccgaa
    27061 tacgctgttc catacttgcc gcgtagtcac gagtcttcgc tttttcttgc gctaactcat
    27121 agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact ctagtgcgtt
    27181 ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac gcatcccttt
    27241 ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg acgggttgtg
    27301 cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac ccatggtctg
    27361 cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc tggtccacca
    27421 gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat gcttatgtct
    27481 atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca acaagggacg
    27541 ggtctgaccc ctgctgagat gcatggttta atcagtggga tgatatgtgg cggtaacgat
    27601 gacagctcat ggctaccgct acttcacgac ctgacgaacg aaggcatggc tttcggtcat
    27661 gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct gcaggatgac
    27721 ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt cgatcgggct
    27781 gatgcattgg caggttgggt caatcacttc ctgcttggtc ttggcgttac gcaaccgaag
    27841 ctggataaag tgaccggcga aaccggtgaa gctatcgacg atctgcgtaa cattgcgcaa
    27901 ctgggttacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga agagatcatc
    27961 gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc gcaaccgacc
    28021 gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg agagtgttat
    28081 gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg agcaaatgca
    28141 acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg ccgacagcga
    28201 atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg aaccggaagc
    28261 ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt ttaaccgcgt
    28321 tcgcgacctg acggcggaga tctggtttgg ccgtcgttta ggtcaggatg ccgcgccaga
    28381 gaaactgggc gttgaccgcg cactggcatt cagcgaaatt aatcagcaac tttatcaatt
    28441 acttaatggt ctggatgtgg tttaccacgc ccagggcgaa tatacctatg ctgatgaaat
    28501 cgtgaacagc gcgctggaaa agctgcgtaa aggttcgcga caaaatctca ccgcgccggc
    28561 aacgatgatc gactggcgtc ccattgttca tgaaatgcgc ctgttcaaat cgccagaaga
    28621 gattgccgta cttcgccgcg cgggtgaaat caccgccctg gcgcataccc gggcgatgga
    28681 aaaatgccgt ccgggaatgt tcgagtacca cctggaaggc gaaattcacc acgaatttaa
    28741 ccgccacggc gcgcgctatc cttcctataa caccattgtc ggcagcggtg aaaacggctg
    28801 cattttgcac tacaccgaaa acgagtgtga actgcgtgac ggtgacctgg tgttgattga
    28861 cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc cggtcaacgg
    28921 caaattcact caggcccagc gtgaaatcta cgacattgtg ctggagtctc tcgaaaccag
    28981 cttgcgcctg tatcgtccgg gaacttccat gcaggaagtc atcggtgaag tggtgcgcat
    29041 catggttagc ggcctggtaa aactcggcat tctgaaaggt gatgttgatg aactgatcgc
    29101 tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag ggctagatgt
    29161 ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg gcatggtact
    29221 gaccgtagag ccagggctgt atattgcgcc ggatgcagaa gtgccagaac aatatcgcgg
    29281 tatcggcatt cgtattgaag acgacattgt gattaccgaa accggtaacg aaaacctcac
    29341 cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggcagctg cgagaaagca
    29401 atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct ggctatttcc
    29461 cggttaagtc acggggcgct gccggtacat ttgattgaag cgaccgcgcc agagtcacat
    29521 gctcatccgg gctttgatgg acgagctatt gcgctagcgg cggggacctg tcagcaactg
    29581 gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac taccgtgcat
    29641 gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta ccaactggcg
    29701 gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc attgctgcga
    29761 aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc ccgtactcag
    29821 agtcacgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt gctgatagcc
    29881 gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca gcaggagcct
    29941 tacgaacaac tggccgtgat tgccaacgtt gcgacttccg ttgcgcacga aggccgcgct
    30001 tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga cggacgctgt
    30061 tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg gagcgacgag
    30121 aagttttgcc gtgaactcca gtcggccttt ggctggcgac ttggaaaaat tacccacgcc
    30181 ggtaaacgca gtgcttatcc gctggcgtta acccgcgccg ccagagctat tacccatcgt
    30241 accgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca agggtttaac
    30301 ctcggtatgc gagatgtcat gagccttgcg gaaaccctaa ctcaggcgca tgagcgcgga
    30361 gaagacatcg gtgattacgg catattgtgc cgctatcaac agcgtcgaca gagcgatcgc
    30421 gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg ttgggcaccg
    30481 ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc ggcacgcgat
    30541 gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta aggaataaac
    30601 aatgcaaagt gttgatgtag ccattgttgg tggcggcatg gtggggctgg cggttgcctg
    30661 cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac cggaacctct
    30721 ggcggcggat gcaccaccac aactgcgcgt ttcggctatc aatgccgcca gcgaaaaatt
    30781 actcacccgt cttggcgtct ggcaggacat tctctctcgt agggccagtt tttatcacgg
    30841 tatggaagtg tgggacaaag acagttttgg tcacatttcg tttgacgatc aaagcatggg
    30901 ctatagccat cttgggcata tcgttgaaaa ctcagtgatt cactacgcgc tgtggaacaa
    30961 agcgcagcag tcgtcagata tcactctgtt ggcccccgca gaattacagc aggtcgcctg
    31021 gggagaaaat gaaactttcc tgacgctgaa agatggcagc atgttaacgg cgcgtctggt
    31081 gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc cgctgacttt
    31141 ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac cgcatgatgc
    31201 ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc ttagcgatcc
    31261 gcatctttgc tcaattgtct ggtcactgtc gccagaggaa gcgcagcgga tgcagcaggc
    31321 aagtgaagat gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc tgggcttatg
    31381 caaggttgag agcgcgcgtc aggtgttccc actgacgggg cgttatgcgc gccagtttgc
    31441 cgcgcaccgt ctggtgctgg tgggcgacgc cgcgcatacc attcacccgc tggcggggca
    31501 gggagtaaat ctcggcttta tggatgctgc agagctgatt gctgaactga aacggttgca
    31561 tcgtcagggg aaagacatcg ggcagtacat ttacctgcgc cggtatgagc gtagccgcaa
    31621 gcacagtgcg gcgctgatgc tggctggtat gcagggattc cgcgatctgt tttccggtac
    31681 caatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca cgcttcctgg
    31741 cgttaaacca caacttatcc gccaggcaat gggattaaac gatttgcctg aatggctgcg
    31801 ttaaaaattt ctcttctgct gtttatttga tacccatcac actttcatct cccggttttt
    31861 tcgccgggag attttcctca t
//
LOCUS       DAPPNJ010000031        31812 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000031 DAPPNJ010000000
VERSION     DAPPNJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.031"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            359..1843
                     /locus_tag="RPM73_001143"
     CDS             359..1843
                     /locus_tag="RPM73_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDX6519525.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2165..2767)
                     /locus_tag="RPM73_001144"
     CDS             complement(2165..2767)
                     /locus_tag="RPM73_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519526.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2854..3060)
                     /locus_tag="RPM73_001145"
     CDS             complement(2854..3060)
                     /locus_tag="RPM73_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX6519527.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3819..3968
                     /locus_tag="RPM73_001146"
     CDS             3819..3968
                     /locus_tag="RPM73_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDX6519528.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(4383..4583)
                     /locus_tag="RPM73_001147"
     CDS             complement(4383..4583)
                     /locus_tag="RPM73_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDX6519529.1"
                     /translation="MVIRKKKCRDCGNAITHNTVYCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            4783..5352
                     /locus_tag="RPM73_001148"
     CDS             4783..5352
                     /locus_tag="RPM73_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDX6519530.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDAGCYATLVHFPVRPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            5519..5920
                     /locus_tag="RPM73_001149"
     CDS             5519..5920
                     /locus_tag="RPM73_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519531.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            5908..6342
                     /locus_tag="RPM73_001150"
     CDS             5908..6342
                     /locus_tag="RPM73_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDX6519532.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRRDILCCVSCGCPLTLKR
                     GNHGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            7117..7343
                     /locus_tag="RPM73_001151"
                     /pseudo
     CDS             7117..7343
                     /locus_tag="RPM73_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(7345..8127)
                     /locus_tag="RPM73_001152"
     CDS             complement(7345..8127)
                     /locus_tag="RPM73_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDX6519533.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            8430..9350
                     /gene="rbsK"
                     /locus_tag="RPM73_001153"
     CDS             8430..9350
                     /gene="rbsK"
                     /locus_tag="RPM73_001153"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDX6519534.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            9378..10694
                     /gene="fucP"
                     /locus_tag="RPM73_001154"
     CDS             9378..10694
                     /gene="fucP"
                     /locus_tag="RPM73_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDX6519535.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            10706..11719
                     /locus_tag="RPM73_001155"
     CDS             10706..11719
                     /locus_tag="RPM73_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDX6519536.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTEQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            12141..12425
                     /locus_tag="RPM73_001156"
     CDS             12141..12425
                     /locus_tag="RPM73_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX6519537.1"
                     /translation="MEQKTLSVEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(12641..13897)
                     /locus_tag="RPM73_001157"
     CDS             complement(12641..13897)
                     /locus_tag="RPM73_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDX6519538.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(13910..14197)
                     /locus_tag="RPM73_001158"
     CDS             complement(13910..14197)
                     /locus_tag="RPM73_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDX6519539.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            complement(14213..14656)
                     /locus_tag="RPM73_001159"
     CDS             complement(14213..14656)
                     /locus_tag="RPM73_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX6519540.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            14927..15958
                     /locus_tag="RPM73_001160"
     CDS             14927..15958
                     /locus_tag="RPM73_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX6519541.1"
                     /translation="MEKIRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            complement(16798..18027)
                     /locus_tag="RPM73_001161"
     CDS             complement(16798..18027)
                     /locus_tag="RPM73_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDX6519542.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(18024..18755)
                     /gene="fabG"
                     /locus_tag="RPM73_001162"
     CDS             complement(18024..18755)
                     /gene="fabG"
                     /locus_tag="RPM73_001162"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDX6519543.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LTSYLMSDIAGYVTRQVISINGGML"
     gene            complement(18755..19219)
                     /locus_tag="RPM73_001163"
     CDS             complement(18755..19219)
                     /locus_tag="RPM73_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HDX6519544.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(19216..20385)
                     /locus_tag="RPM73_001164"
     CDS             complement(19216..20385)
                     /locus_tag="RPM73_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HDX6519545.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(20387..20971)
                     /locus_tag="RPM73_001165"
     CDS             complement(20387..20971)
                     /locus_tag="RPM73_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HDX6519546.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            complement(20968..23286)
                     /locus_tag="RPM73_001166"
     CDS             complement(20968..23286)
                     /locus_tag="RPM73_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HDX6519547.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(23255..23860)
                     /locus_tag="RPM73_001167"
     CDS             complement(23255..23860)
                     /locus_tag="RPM73_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HDX6519548.1"
                     /translation="MKFLPLLVLLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDHTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            complement(23857..24279)
                     /locus_tag="RPM73_001168"
     CDS             complement(23857..24279)
                     /locus_tag="RPM73_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDX6519549.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            complement(24283..25959)
                     /locus_tag="RPM73_001169"
     CDS             complement(24283..25959)
                     /locus_tag="RPM73_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX6519550.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(25950..26303)
                     /locus_tag="RPM73_001170"
     CDS             complement(25950..26303)
                     /locus_tag="RPM73_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HDX6519551.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(26290..27648)
                     /locus_tag="RPM73_001171"
     CDS             complement(26290..27648)
                     /locus_tag="RPM73_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDX6519552.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            complement(27645..28226)
                     /locus_tag="RPM73_001172"
     CDS             complement(27645..28226)
                     /locus_tag="RPM73_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519553.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            complement(28231..28482)
                     /locus_tag="RPM73_001173"
     CDS             complement(28231..28482)
                     /locus_tag="RPM73_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX6519554.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            complement(28494..28751)
                     /locus_tag="RPM73_001174"
     CDS             complement(28494..28751)
                     /locus_tag="RPM73_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX6519555.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(28726..29547)
                     /locus_tag="RPM73_001175"
     CDS             complement(28726..29547)
                     /locus_tag="RPM73_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDX6519556.1"
                     /translation="MSKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(29544..30266)
                     /locus_tag="RPM73_001176"
     CDS             complement(29544..30266)
                     /locus_tag="RPM73_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HDX6519557.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            complement(30307..31365)
                     /locus_tag="RPM73_001177"
     CDS             complement(30307..31365)
                     /locus_tag="RPM73_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDX6519558.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(31430..31774)
                     /locus_tag="RPM73_001178"
     CDS             complement(31430..31774)
                     /locus_tag="RPM73_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519559.1"
                     /translation="MLSACARTAPVQQISTTVSVGHTQEQVKNAILKAGAQRKWIMTQ
                     VSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDGKIHKNYNRWVRNLDK
                     DIQVNLSTGATL"
BASE COUNT         7970 a         7853 c         8177 g         7812 t
ORIGIN      
        1 agtgcaggaa ctgtagtctt ctttatttta ttcttcttca ggaggggggg attcctgaag
       61 gcgttattcc cggtgttctg tttatgattc atcccggcag gaaaccggta tctgcaatac
      121 ccgggatgat gctttttgtt cgtacgtggt tgtgtaaaat ttgaatagtg gatgtgctct
      181 gaagggaagg aggggacaca cagattcatt ttgtctttct tcagcagcaa aacacactgt
      241 ggttattatt taaccattaa atcaacaaaa atttttacct catattgatt tgactgctat
      301 ttttgctatt aatagccaga attgcagtgc ttttatatct gtgtgtagtg aggttactat
      361 gtcattcggt cagccttgtg atgagtttcc cctgagttcg ctgcctccac tcattcgtga
      421 tgccgttatt gaagctcagc aaattacgca ggctcccctt gggcttgtgg cagcgtctgc
      481 cctgggcgct gtttcacttg tctgccagaa tctgattgat gtctgcagac tgaatacact
      541 gcgggggccg gtctctcttt ttttcctgac gcttgccgag tccggagaac gaaaaaccgc
      601 agtggacaaa ttattaatga agcccctgta ccagcaggag atgcagttat attccagata
      661 caaaagtgaa ctggctgtct ggaaaaacaa ggaagagctt ttaaaggctc agaaaaaagc
      721 attgttgtca aaactgaata aagaactccg caaaggcgcg gatgagtcag aaaccctcag
      781 acaactggaa gtacttcaga aaaacagtgc ggaagaacct gtgaggtata aattcatttt
      841 taatgatgcc acgactgcgg caattaagaa tcagctttgt ggtaaatggc gttcagtggg
      901 tattatgtca gatgaagccg gtattatatt tgatgggtat accctgtctg agttgccttt
      961 tattaacaaa atgtgggatg gctctgtatt gtctgtggac agaaagaatg aacccgaaca
     1021 gatgattgaa aatgccagaa tgacgttatc cctcatggtg caacccggtc tttttgaccg
     1081 atatatggag cgaaaaggtt ctgttgccag ggacagtggt tttcttgccc gttgtcttat
     1141 cagtaaacct gcaaccacgc aggggaaacg ctttattaac ggagctgtga ttccgggagg
     1201 atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta ttgaaaaatc
     1261 cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa tatttattga
     1321 gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc cgtttcgggg
     1381 acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc agtatttcag
     1441 ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa taagttcgtg
     1501 gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac tgcagcagaa
     1561 ggacgccgag gagctgttcg actggctcat tgaggagtgc cgcggagaat gcccgccccg
     1621 tgtcaggaaa aattatattc tccagtgtgg cccgggccgg ttcagaaaca ggaaaaaact
     1681 gaacgcattg ctgaacattc ttgaatctca gttcaggctt tctgtcgtgc cggaaggaaa
     1741 aaccatgtat gttctgcttc cccagatagc cagtttaaag ttgtctgatg tctctggtat
     1801 ttttacctcg ggttatcact acaataagct gagagcgaag taaccggtga gcagtttttc
     1861 tccattatgc ttcagactta gatgattgat tattatgggg atttcatcac cgatattcag
     1921 atgtatgaat atcggtgatg aactaaagct caggagctgc gtggtggtga tgaatcaaca
     1981 ggcatataac tgatacccgc cggtcagaag ttccagcctg cgactttcct gtctgcgtgc
     2041 ccgcagtccg gcgttcaggt gcagcgtgtg acagaacgca cggagagcga aacggcaggc
     2101 agacaggccg ttcagtcagc cctggcaccc tgcccggata ccagcttccc tgaaacaact
     2161 ggcatcaggt gcgcttatcg tcaaccgttg tgcggtatgc caggagcacg tactgatcca
     2221 tcagatccgt cagtgctgcg ctctgcttct gacggttgat gatccattcc tccagctctt
     2281 tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag gcagtttccg gtcattaaat
     2341 caagccagca aagccagacc agtttgaggc gcaactccgt gccaccaaac tgacggaaga
     2401 ggctgatcag tttatttgcc agcgaacgct gcctcagacg ttcacattca gacaggagaa
     2461 tgccttccag catcggatga tgaaccagca gatgttcact ggtgcataaa ttcactatca
     2521 gatcatcatc caggggcggc gtggtccgtt tgtccggacc ataaatcagc cggtgcaggc
     2581 tctggggtac gatggcaatc tccggttttt caccattgaa tgacacccac ccgggcgtca
     2641 gattatggaa gtagtagata ttcggatgac acacgcccca ggatacggcg tgccgggtga
     2701 agccggtcag ccatttctgg tagctttcgt ggagcagctc gtaacgggtc tgatactgtg
     2761 gtttcatttt catattcctc acgggggata acaggcagca gaacgccgcc ggcaggccgg
     2821 agactgcgtc tgttgcagat gaacggttaa gaattacgga cgggactgtg caatacgcgc
     2881 ctgcagccag gcttccactt cgcttttcag ccatcgggag ctgcggccca ttttgatggg
     2941 cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac ttatcggtca ggccggtcag
     3001 ttgagtgata aatgccatgt cgaccatctg gtcatccatc agtgaaactg gggtgatcat
     3061 gagaatgttc ctccggatgt tgttaaacca gccggtgtgg tcatgaaaac aacgcaccac
     3121 cttcaccggc ataacaaccg gggagaaacg taaaaaataa tcgggaatgt gaccgccagg
     3181 agataaacgg ggaggttacg gtggctgagc cgtaccggta tgccgggtgg ttgttacgaa
     3241 acgatgcttc cgcacgcgcg cgcgataaag aagggatgat tttcgtgagg aagaggggaa
     3301 aactggcgtt ctgataattg cagagttatt tacggggcgg ttacgatggg gcagaccgtg
     3361 tccggtgggc aggcgacagg tatcaccgat gggataagac tgtctgattg gtattataaa
     3421 tatataagag agatcaaaca catgagatca aatatatgag ttcatatatg tgaaataaac
     3481 acggtgagtg tgatcagtgg gcatgatgag aacgcagtag caacagactg cgctatccct
     3541 gatagctggc gtataacccg cgtatataaa acgtatctgc aatgtacgtg aacagagtgc
     3601 attaagcatc cgtctgagcg gtaaacatat ccgacctggt gagtgacagt ctgagagacc
     3661 ggttaagtgg tggctcggga gtaatgagtt tggggtgggt accagaatgc cagtctggcg
     3721 gtatgctgac agtatcacgt taattgcagc ataaagtgat gagaggctaa tggaagttaa
     3781 aaccagagca gttccgtcgt tgttcatccc gggagacgct ggaaaaagtg atttcccaca
     3841 cgcgttataa gcttactcca gcggagatgg aagccttcaa ctctgcggtc gatcaccgac
     3901 tggcagaact gacaatgaac aagctttacg atcgtgttcc cgcttcagtc tggaaatatg
     3961 tcatctgagc gttcggtaat cacaactgca tcgcgccagg tgttatccgc tttgtgattc
     4021 ttattggctc ttctcaggtt attaaaagct gaatatgttc ctgttgtcgg gaaccaggat
     4081 aataccggga acgttaagcg gtatagcgac aacaaagaat tcgaaatcaa aagggaatgg
     4141 tgccggaggc gtgggcggta atgggctgat tttcaggtat taccggctta cagcgtgtgg
     4201 ttgatattga ggggctgtga gtttcacgta tttgtcagaa acccagacga gttttcctgt
     4261 atgggacgaa ataaggcagc ggcaatacat ggaaaaattt aatatggtat gaaggagaac
     4321 ctgtcggtta cgcttaatat aaccggtaca ctgtcagctc aggggataca tgaaccgtta
     4381 tctcaccagc tacacagtat atacacactg acgctgacag tagtcagcag gaccacaata
     4441 atcagtgcca gcagtatggt aacgatacgg tctgtattcc ggtaatagcc gaaggggtcg
     4501 acagaaccac aatacgggca atacacagta ttatgagtaa tcgcgtttcc acagtccctg
     4561 cacttctttt tgcgtatcac cataacgccc tcctcacagc cttacccgta accggttttt
     4621 tacattaaaa aaacaacgct gaaaattcaa ccgtcagtcc ggagacgacc gttcgggtta
     4681 tcacagagtg cctgagacag tgccctgccg gaggtcactt cagggactct gcgcattttt
     4741 tacgacgtgg ctattccgta tgaaccatac ggagatttaa ccatgaccta caaatacaac
     4801 cccttctggc agcaacgtat tcgtgagaca gtgcggcacg cactgaatgt tcatccccgc
     4861 ctgacggcat tgcgggttga cctgcgtttc ccggatgtac cggcagcaac ggacgcagct
     4921 gtgatatccc gcttcatcaa tgccctgaaa gcccgaatcg acgcttacca gaaacgtaag
     4981 catcgggaag gtaaacgcgt gcatcccacc actctgcatt acgtctgggc ccgggagttt
     5041 ggggagtgca aaggtaaaaa acactatcat ctgatgctgc tggtcaaccg ggatacctgg
     5101 tgtcgtgccg gcgattaccg cgatccgggg tcactggccg gaatgattaa acaggcctgg
     5161 tgcagtgccc tgggggtgga tgccggatgt tatgccacgc tggtgcattt tccggtcagg
     5221 ccggcggtgt ggctggaacg cgatgatgaa accggctttc agcaggtgct ggaacgtgct
     5281 ggctatctgg cgaaggagca taccaaagct cgcggcaccg gtgagcgcaa ctttggctgc
     5341 agtcgtggct gagcccgaca gacgacttat ctgaccttag tgtgatgacg cgttcacagc
     5401 gaccacactg attttccctt ctgagactga actgaaaacc gctattcaca cctgaccacg
     5461 ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg cggctgagga atatgacgat
     5521 gtacgcaaaa tcctttcttg cacttgatgg caacgggcgt ctgactggtg cccgtaccgc
     5581 acagactgca ccttacgatc gctacacttg ccacctttgc ggcagtgcac tcagatacca
     5641 cccgcaatac gacactgaac gtccctggtt tgaacacact gacgacgggc tgacagcgca
     5701 cggtcagcag tgtccttatg tcagaccgga acgcagggag gtacgcttga ttaagcgtct
     5761 gcagcaattc gtaccggatg ccttacccgt ggtgcgtaaa gccagctggc actgcagaca
     5821 atgtcaccac gattattatg gggagcagta ctgcacaaac tgccagaccg gaggattcag
     5881 tattccccgg acaactcagg aggaaatatg cgaattctga actgttatat ggcgaatgac
     5941 agcaaaggcc attttgttac ggcgaaagaa gcagcgaagc acaaccgccg ggacattttg
     6001 tgctgtgtgt cctgtggatg ccctttaaca cttaagcggg gcaatcacgg acaaccaccg
     6061 tggtttgaac atgaccagat gactgttgct gcgaaaatcc tgctgcgatg cacctggctt
     6121 gacccggcag agaaagaggc ccgtcgtttg catctgcagg gtatgacggt tccggattat
     6181 acggtgaagg tgagaaagtg gttttgtgtg atgtgtgacg aagattatga aggagaaaag
     6241 tgctgcccgc gttgtggtac cggggtatac agcagggcgt gggggcggca ggaggtgccg
     6301 tcggaagatg ccagggctga taatccgtta cagaggctgt aatggttgcc tccggcgcag
     6361 tttgcggtga tgccgttccc gggaatggat tgccagtgcg gggactgtgg agcttaattc
     6421 cggtattggt ggcctttccg ttgattttga gccaacagcc ctcctgtgac tgacaggctg
     6481 cctcgtcatt ccattcgtat ctccagtaac agggggttgt attgtatttt aatgtgccgg
     6541 ttcaggcgtg cgtttcccgg cgcgtctgca ccggcttaac caaattcaac agggataaaa
     6601 tagtgtaaat aaccgaagta ttacttgcag aatcggggga agcatccggg gttttattca
     6661 acaggcatgg gaggctccgg ctctgacaga cattgtactg tggaatgttg ttacaggttg
     6721 gggcggcaac cgtgccagga gaccatgagc cgttcacggc attaaaggcc acacttcagt
     6781 cgtttacatt ccctgtggtg gcaggcatct tccgttaagg cgtcagagcg ttaccaacgt
     6841 tatgttgtca tggctcacct ttcatagtcg atgcttatct ggtgactgaa actagccgca
     6901 gataaagagt gtgaagttga taccatggag tggagtgacg tgcaggcagg agattcgctc
     6961 tatgcagggt tgtgagatga attcaaaccg gctgtcagcg ccagtaatat ttgaagattc
     7021 gtccggttgt tatccagtgt gcattaaaaa cccagacatt atggacaata caacggcttc
     7081 cataacaggg aggagcctgt ttattatccg ggcgtaatga ctaacctttg tgggcttttt
     7141 cccgtcgtgc ttttaacgtt gaaggcattt tctgcctgcc gattcaggac agcgacaaaa
     7201 gccatatcag actattggtc aatgatgacc agcgtctgga gtagatgata agccaaattg
     7261 ataaactgga atatgtgatg aaagtgcaac gtaatcagtc caatccgacg atgtttaaca
     7321 agagtacggt gttttttcag taagtcattc gtcttctgaa tcaggtgtaa tgatctttac
     7381 agaagcattc cggcagtgcg taacataatc tgccggtaac ggacgctcgc taatcagaac
     7441 atcaaatgca gagagcggtg cgaggctggc agaggctact tcatcgaaca aggcgtggcg
     7501 ggcgagcaaa attttcctta gtccacgtgc catcgcttta cgcttagtgg caagatcttc
     7561 gggattaaac cagctgacgc caaagtgatc atgtacaccg ctggcggaaa taaatatttt
     7621 tcgcggattg agagagtcta atggcgaaga gttactggca tcgtaaaaag catcacttct
     7681 ggcacgatac gtaccaccac aaagtattgc tgtcacatta ggtttttcat tcaacgcaac
     7741 aaagacacga tgtgagtaac agatgccagt gaaggtgatt gcatccggga tcatgcttat
     7801 aacgagcggt atctcctggc cattatcaaa gaagatcaga tcattttcat taaccattcc
     7861 ggcggccaga attgcaatag gtaagtcatc acgatgattt ctcggaacgt cctggattac
     7921 tggcatggat ggtgcgggtt tatgcaccat tacaatatag ccacccagta gggtcagtgg
     7981 cagaggttca tcttcctgat ggagatcgcg acgaatagtc attacagaaa cttccagcat
     8041 tcgtgccgcg tctttcaaat ggattctgtc ggttttctta agtatttcaa tcaaacgtcg
     8101 gatacgctct ttttgcttcg tttccatcaa ctctctccgt aaaatgttat aaaaataaca
     8161 ttatggcatt agtaaacatt gttttagtta ccatactata cgaccgaccc tttgaaatga
     8221 aaagggtata gactgctgaa ttttcggtat taatcctgtt aggttgctta tgattagata
     8281 gtgttggcct gagtgataag actggaagtc tattaaataa acatcgggtt gatgtgctaa
     8341 tcgcgatctc agtcacaaat gatgtaaaag taacatgcta atgttattta aataccattg
     8401 ttatgcgaaa gtttataaga ggtagtgaaa tggacattgc agttattggt tccaatatgg
     8461 tggatctcat tacttacact aatcagatgc ctaaagaagg agaaactctg gaagcacctg
     8521 ccttcaaaat cggatgtggt gggaaaggtg ctaatcaagc ggtggcggcg gctaaattga
     8581 attcaaaagt cctgatgctg actaaagtag gtgatgatat ttttgcggat aatactattc
     8641 gtaatctcga atcatgggga attaatacca cttatgttga gaaagtcccc tgtaccagca
     8701 gtggtgttgc accgatcttt gttaatgcca actcgagcaa tagtatatta ataattaagg
     8761 gagcgaataa atttctttcc ccggaagata tcgatcgagc tgcagaagat ctgaaaaaat
     8821 gcaaattgat cgttttacaa ctggaagttc aactggaaac agtttaccac gcgattgagt
     8881 ttggtaagaa aaatggtatt gaagtgttat taaatccggc tccggcattg cgtgagttag
     8941 atatgtctta tgcctgcaaa tgtgatttct ttatcccgaa tgaaacagaa ctggaaatat
     9001 taaccggtat gtcggttgat acttatgatc atattcgttt ggccgcacgt tcgttagttg
     9061 ataaagggct gaacaatatt attgttacca tgagcgaaaa aggcgcatta tggatgaccc
     9121 gcgatcagga agtccatgtt cctgcattca aagtaaatgc tgttgatact agtggcgctg
     9181 gtgacgcatt tattggttgc ttttcccatt attatgttca aagcggtgat gttgaagccg
     9241 ctttgaaaaa agccgctctc tttgccgctt tcagcgtcac agggaaaggt acgcaatcat
     9301 cttatccgag tattgaacaa tttaatgaat ttttaacctt aaacgaataa tcatcctgca
     9361 cagttaaagg tagcactatg aacgataaaa acatcattca gatgcctgat gggtatctga
     9421 ataagactcc cttgttccag tttattttgt tatcgtgttt attcccgcta tggggatgcg
     9481 cagctgcatt aaatgacata ttaatcactc aattcaaaag tgtattttcg ctaagtaact
     9541 ttgcttcagc attggtgcag agcgcatttt atggaggtta ttttttaatt gcaataccag
     9601 catcccttgt gattaaaaag accagttata aggtggcaat tttaattggc ctgacgctgt
     9661 atattggtgg ttgtacactt tttttccctg cctcgcatat ggcaacctac actatgttcc
     9721 ttgcagctat ttttgcgatt gcgattggcc tgagtttcct tgaaactgca gcgaacacat
     9781 acagttcaat gatcggtcca aaagcctatg ccacattgcg gctcaatatc agccagacct
     9841 tttatccgat tggtgctgcg tctggtattt tattgggcaa atatctggtt ttctccgagg
     9901 gggaaagcct tgaaaagcag atgtcaggaa tgaacgccga gcaaattcac aactttaagg
     9961 tgctgatgtt agagaacacc cttgaaccgt ataagtatat gattatgatt ctggttgtgg
    10021 tcatggtgtt gttcctttta acccgcttcc caacctgtaa agtggcacag acaagtcacc
    10081 ataaacgccc gtcagcaatg gatacgctgc gctatctggc tagaaatccg cgctttcgtc
    10141 gcggtattgt ggcgcagttc ctttatgtgg gtatgcaggt cgcagtatgg tcatttacca
    10201 tccgtctggc gctggagttg ggcgatatca acgaacgtga tgcttcaaac ttcatggtct
    10261 atagctttgc ctgcttcttt attggcaagt ttatagccaa tatccttatg acgcgtttta
    10321 atccggaaaa agtattgatt ctctattccg ttataggggc gctgttcctg gcatatgttg
    10381 cactggctcc aagtttcagc gcggtctatg tcgccgtcct ggtaagtgtt ttgtttggcc
    10441 cttgctgggc gacgatctat gcaggaacac tggatacagt tgataacgaa catacagaaa
    10501 tggcaggagc agtcattgtt atggctattg tcggtgccgc ggtggttccg gcaattcagg
    10561 gatatattgc agatatgttc cactcactac agctttcatt cctggtttct atgctctgct
    10621 ttgtctatgt cggagtctac ttctggcgtg aaagcaaagt acgtaccgca ctggctgaag
    10681 tcacagcgtc ctgaggagaa ccgttatgag tacgcgtatt aatttgtggc gggcgctttt
    10741 tggcgagaaa cctcgtatct tgctggaaaa tagtgatttt actgtcacgt cttttcgata
    10801 tgacagcggt gtcgaggggc ttaaaattgc aaacagtcgt ggacatttga ttattcttcc
    10861 ctggatgggg cagatgattt gggatgcgca atttgatgga cacggcctca ccatgtgcaa
    10921 tatgtttcgc cagcctaaac cggcgacaga ggtgatagag acctatggct gttttgcttt
    10981 tcattcaggt ttgctggcta atggctgccc atctgcggaa gatactcatc tgctacacgg
    11041 tgaaatggcc tgtgccgcga tggatgaagc atggctggaa ttagacggag atatgttacg
    11101 ccttaacggg cgttacgagt atgtaatggg tttcggtcat cattatctgg ctcagccaac
    11161 agtggtcctt cacaaatcct ctacactgtt cgatattaag atggccgtaa ccaatctggc
    11221 ttctgtagat atgccactac aatatatgtg ccacatgaat tatgcatata taccaaatgc
    11281 tacattcagc cagaatatcc cggacgaaat attaagattg cgtgaatcag tgccttcgca
    11341 tgttaatccc accgctcagt ggctggcgtt taatcaacgg attatgcagg gagaagcctc
    11401 tctgtcgacg ctgagtcaac cggaatttta tgatccagaa attgtcttct ttgcagacaa
    11461 actggacgct tatacagagc agccagagtt tcgcatgatc tctcccgatg gcactacgtt
    11521 tgttactcgt ttctacagtg ctgaactaaa ttatgtgaca cgctggatcc tttataacgg
    11581 agagcaacag gtagccgcct ttgcattacc tgcaacctgt cggcctgagg ggtatcttgc
    11641 cgcgcaacgt aatggcacgc tgattcaggt tgcaccgcag caaacgcgca cttttacagt
    11701 caccacagga attgaataac cactcaaggc ctgaacagcg gcgcgcttat cgttaaggta
    11761 agcgcgtttt tttacccgcc aggacaagac tatgatagcg ttgcccttat agacgatcac
    11821 attatcgtcc actccaaatt tgcgcaacta ctgtagagtg gtgtactcca cagcttcttt
    11881 gaggagctta acctccattg ttttcttacc gagcaagcac tgcaactctt ttatttattt
    11941 gtagtggtca acaaaaactg gtcacaattt cagttttttt ccagtatcta ttttccgatt
    12001 cgttcggcgg taatccaccg ttatattcat gaggccggag tgcactataa aagtgtatgc
    12061 gtaaaccgac tgctgtatgt agccattaag ccagtattgg taacataggt acccaccttt
    12121 tcaactagtg gacacctgtt atggaacaaa aaacattatc agttgaaccc cgcaggtcat
    12181 tttcaaatga gtttaaactt caaatggtta aactggcttt acaaccaagg ggttctgttg
    12241 cccgtattgc ccgggagcat gatatcaatg ataatcttct gttcaaatgg ctcaggctct
    12301 cgctgatcaa agggcgtata tcacggcgtc ttccggtaac gaactcttct ggcattggcg
    12361 ttgaattatt acctgtggag atgacgctgg atgagcggcc ttaccccgtt ttttacgcga
    12421 ggtaattatg aagatcaatg actagccgtc gaaccgtgtg catgaactgc tgcactgaaa
    12481 cttctcagct gtaaaataat ccttacgcta cgtacttctc ggagcgctta cgattaatcg
    12541 gatgcagtcc tgactggaag aaagaaaaat ggagaaaata agaggaatta atatcctcca
    12601 cgttcctgaa atcctggagg atattaatgt catcaaaata ttagcgaacg ataatcccta
    12661 acaaaatacc cagtgcgcca aagtcagcat cactgaaggt ggtgttagca aaaccaatat
    12721 cacccagcac cggcaataag aatactggca ggaaggtaat aagcaagccc tgagcgaagg
    12781 cacccaaaat cgcaccacga cgtccacccg ttgcattacc gaatacccct gcagctgcac
    12841 ctacgaagaa gtgcggcacc acaccaggaa taatcacgat catgttcagc agatacaacg
    12901 tgaacatacc gattaaacct gcagcaaagc tactcaggaa accaaccagt acagcgttag
    12961 gtgcgtaagg gaagacgacc gggcaatcga gagcaggtct ggcattcgga accagcttgt
    13021 ctgaaatacc tttgaatgcc gggacaatct ccgcaatcac catgcgcaca ccctgcagaa
    13081 tgatgtacac gccagctgcg aaggtaatgg attgcatgat agagaacatg aaccagtttt
    13141 taccaccgct aagctctttc accgcatctg cacccgcaaa caggcacgtc accaggaaaa
    13201 ttatgctcat ggtaaaagag atagcaaccg gcgtgtcgcg caagaacagc agacttttcg
    13261 gcacattcat gtcttcagtc gagtgctctt tattaccaaa cttgctgccg atgaaaccgg
    13321 ccaatacata agataatgtt gagaagtggc cgatggcaac gtcatcagaa ccggtcactt
    13381 tcttcatgta cggatgcgca atagccggga aaaagaccat tgccacgcca acaaccaaag
    13441 agccaacggc tataagcgta atgccggtca tgcctgctgt tgccagaatt accgctacca
    13501 tcatcgacat aaacaacgtg tgatgaccgg tcaggaagat aaatttccac ggtgtgaaac
    13561 gggcaatcat aatgttgata accatcgcaa agaacataat catcgccatt tctttcccga
    13621 agcttttctg cgctacggaa acaatggctt cattgttcgg cactacaccc tgaataccaa
    13681 aggcatgctg gaaaatgttt gcgaagtcac ccagtgacga cacaactaaa cccgcccctg
    13741 cgcctaaaat cacaaacccc ataatggtct tgacggtacc tttaatacat tcggttaccg
    13801 gttttttctg tgcaatcagg ccaatcaggg cgattaaacc gaccagcact gcgggttcgg
    13861 aaagtacatc gctcatcaaa aaacgaaaga aatccatatc gcccccagct tacagtgcgc
    13921 ctaattcggt taataccacg gacaaacgtt ctctcatggc tttcttatca atcatatttt
    13981 ccagtgcgac gattttgcct gcgacagact gcgcggctaa ctgctcggca atgtctttgg
    14041 tgccgacata gatatcactc ggcgtccctt tcgcggagcc caggtcgacg tgatcaacat
    14101 cagcgctgac ggacaaatcc ttcaaaatgc ttttaatgct catttccatc atcaggctgg
    14161 tgcctaagcc gttaccgcaa accactgtaa ttttcataaa tgttcctctg gattaatagc
    14221 gcgcaataac agacaaaatg tcctgcgtcg ttttggccgt caaaatggcc tgtatgtctt
    14281 tttcgttatc gaataacttg gcgagttgag aaatcgcttc aatgtggctg gtactgtcgg
    14341 ttgcagccag cacaatcagt aatttcactg gatcgttttc atcggcatca aaattcacac
    14401 ctgaagggat cagggtcagt gctaatgaca gcttgttagc cccctcttcc gggcgcgcat
    14461 gaggcatcgc aataccaggg ccaaccacat agtacggacc gatagtgtcg tgcgagtgat
    14521 agatcgcatc aacataacgc ggttcgatag agccgttttc aatcaacggc cggcatgaaa
    14581 tcgccacggc ttcccgccag tctttgacct gttccacgac ctgaacgacc tctgtattta
    14641 atagattttt taacatgcta ctgataactc ccgatgataa ttaacaggag agtagatatt
    14701 ttacgcatta attaatgtga tgattgtcgc atttgatagc gctatctgat aacgctacca
    14761 atctttgtga tagcgctatc atttctctgc cgccatgctt tggctatatt cggttcctgc
    14821 atatacaatg caacaggata tttgctaccg tgggcaaaaa ctggaaagag taatggtgaa
    14881 ataaaagtaa tgaaaacagc tggttgtaat tatccatgga gtcgaaatgg aaaaaatacg
    14941 taagcgccgt ggtaccggac gggtgacgct gcaggaggtc gctaactttg caggtgtcgg
    15001 aaccatgacc gtttcccgcg cgctgagaac accggaacag gtttctgaca agctgaggga
    15061 aaagatagaa caggccgtag aagagttggg ttatattccc aacagaactg ctggcgccct
    15121 tgcatctggt cacagtcata cagtcgccgt actggttcct tccctgacag ataaagccag
    15181 ctcccgcttt atgcagtcat tacagcaggt gcttaataaa aatgaattcc agctactgct
    15241 tgggtgtcat gagtataatc agctcaaaga agctgaaatt ctgatgacgc ttcttcaagg
    15301 caacccggcg gcgttagtta tttttggttc tcagttggct gataaaactc accagatact
    15361 cgagaaaaca aatataccta ccattaacgt tatcggttcc cctttcagtc cggctcaaat
    15421 taccatcgaa acagcatttt ttgaagcatc acataaactg accgaacatc tgctggagca
    15481 gggttataag aatatcggat ttatcggtgc gcatatggac aaccgcctgc aacgtcagca
    15541 actgaacggc tggcataaag caatgctgag ccattaccag aacactgatc ttgttatcac
    15601 catgccggat gccgcaagtc tgcaattggg gcgttacgca ttaaacgaga tattactgcg
    15661 acaaccagag ctggatgctg tgatttgcag ccacgaagag attgcgctgg gtataatgtt
    15721 tgagtgccag cggcgtttgt taaaaatccc tggaaatatc gctgtagctt gtcttgacgg
    15781 ttcagacaat tgcgatcaaa cacacccaac tttgacatcc atccgtattg actacaaaaa
    15841 aatggggata gagacaggta agttactgat tggactattg aataataatc atgatgaatc
    15901 agaagagtcg cgtatcgtgc aattcaacta ccagatagaa ttacgtcaga gtacatgagc
    15961 ctaaaagagc gctattacaa agttagctat tgtggtaggg atagccgttt ccggctattc
    16021 cccgcgcagt tccggatgta tgctgctaac gcatccattc ctacttcagg tatttggcgt
    16081 agaagcggat gctaaaaact tgtcgcaaaa ccgcaatatc agcctgcagt atcgctctcc
    16141 agattatcca ttcctatatt gatgacaacg acaggtaaga ctgtgactca ccaaacttgg
    16201 atgtttttct gagcttaaag ctcgttgcgt gaataaggcg agagtcagcg aatctcattg
    16261 aggcccgcag caccggctgc aataaggata tagagcagca atctaccgtc aacgtcaaaa
    16321 acacggaagc cttgcaaacg ggatgcgttc atataaacaa catggagcag caacagtacg
    16381 atgcctgttg ttgctcagtc aggacctttt ctgccatgcg cgagccagcg tcacgattga
    16441 taaataatgt tcgcaactga ttactttact gcaacagacg tggttggtaa taagcactgg
    16501 cggacaatgc aggatggaag gctgttttca ggttgtccgt tcaggtacgt tagatcagtt
    16561 cgttgcgcta aagacaactg ttcagtccct tatgttaccc gttatggcaa ccattgcccg
    16621 gtgataacct ggcgtgtcat ctgtgcatcc gttgattcgt gtggactgat aaagttttgt
    16681 ctgctgaatc gttatacgga atgctcttca gtatgcgtaa gagaatttga ggttcatatt
    16741 cattgattca gattttttga agaatcacca catgggatgt ccatgtggtt gtataactca
    16801 gggccagcgc ttaataatga tggatgtatt aatgccgcca aaggcaaagt tgttactctg
    16861 caaaaactca caatcaacct tgcgggcttc atgcatgatg taatccagcg cgccacaatt
    16921 cgggtctggt ttattcaggt ttagcgtagg cgcaaaccaa ccttcgcgca tcatttgcag
    16981 actcatccag gcttcgagcg cgccacaggc accaagcgta tgaccaaaat aacttttcag
    17041 cgaggagagc ggcacattat caccgtaaat ggttgctgtc gccagacttt ctgccatatc
    17101 accgcgatcg gtcgccgtac catgcgctga aatatagccg atatcttgtg cgcttaaccc
    17161 ggcgattttc agcgattgct ccatacaata ctgcatggtt tcgcgctgcg gctgggtaat
    17221 atgtgccgcg tcgcagttcg tcgcaaagcc gacgatttca ccgtaaatgg tcgcgccgcg
    17281 cgctttggcg tgctccagtt cttcgagaat gagcgtacct gcgccttcac caatcaccag
    17341 cccgtcgcgg ttttcatcga acggtgaagg tgtggttttc ggcgcatcat tgtgctggct
    17401 ggtggcgaaa agcgtatcaa ataccgctgc ttctgaagga cataactctt cggcaccacc
    17461 agcaaccatc accgtttgat aaccgtggcg aatggcttcc cacgcataac cgattgcctg
    17521 gctgccggaa gtacaggcgc tggaggttgg gatcacccgc ccgcgcaggc cgaaaaagag
    17581 gccagtatta acggcggtgg tgtgcggcat catctgcaca taggtggtgc cagtgatgtt
    17641 attggtgtgc ttttccgtca gcatggtggc gaactcgctt accgggccgg tactgccggt
    17701 cgatgaaccg tacgcaatgc ccgtttcgcc gctggttaat atcggatcgc caatcaaccc
    17761 tgcctgctct aacgccagct cgctggcacg ggtcgacatt tgcgacacgc ggcccatcgc
    17821 acggatacgc ttgcgggtgt agtgctccgg tagcgtgaaa tcatcaacag gcgcgccaag
    17881 cagggtgtgc agcccatcgt agacctgcca ctccggcatt ttgcgcaccg cattttcata
    17941 tgccagtaag cgtgcagaaa cgtcctgcca gttttcacca aaggcagtca cgccgcccat
    18001 tcctgtaatc actacgcgac gtgtcataac atccctccgt tgatggaaat aacctggcgg
    18061 gtgacgtaac ccgcaatatc agacattaaa tagctggtaa gcccggcaac ttcttccgcc
    18121 tggcccatgc gtttcatggg gatcatcgac attgcctctt tcagggcgct ctcttccatt
    18181 tcgatcatcc cggtatcaat aagccccggc gcgatgcagt tgacggtgat tttgcgcttc
    18241 gcgagttcga tagccagcgc ttttgttgcg ccgataattc cggctttggc tgcgctgtaa
    18301 ttcacctggc cgcgattgcc cattacgccg gaaaccgagg acagcgtgat gatgcgccca
    18361 ccctggcgcg cgccgatcat tggcataatg cacggctgaa tgacgttgta gaaactgtcg
    18421 aggttggtgt ggatcaccgc atcccagtcg tcatcgctta aagccggaaa agcggcatcg
    18481 cgggcgatcc cggcgttact gaccacgccg taccaggcac cgtgttgcgc gatctcatgc
    18541 tccagcactt cccgacactg ttcgcggttt gcgacgtcga aactgagcaa acgcccgtta
    18601 cccccgttgg cgacaatggc attcagcgtt tcctgagcgc ctgtggcgtc acggtgatag
    18661 tgaacgccaa tgttaaagcc gtctgccgcc agttggcagg cgatggcgcg accaatgcct
    18721 ttgctggcac cagtaaccag aactgaacga ctcatcagga cgctccttgt tgaaacaggg
    18781 tggtaagttc ttccgcggtc ggctggaagg tgttaatgcg gcccgtcgcc tcgccgtcgt
    18841 tgatgctgca ctcaaaactg ccgaagcgat cgtcctgcat cagcagtttg acggtaatgg
    18901 tcagcgtctg gcctgcgggc agaatgccag cggcgcaaag caattcacgc gcgcccagta
    18961 tcatccctaa ctcaatactg tttttgccct gctggtggcg atgccagcca gaccagacgc
    19021 cgacggtttg cgccatcagt tcaagggcaa accagccagg gagattgccg tccgggtcga
    19081 gaaacggtgc cagtacgcca ctgggcgaaa ccgtcacgcg gcacaccgcg ctgtcatcgc
    19141 tgacgctcac cacgtcttcc agcagtaaca tgggcgcgtc gtggggcaga taagcgccgg
    19201 gggataaata gtggctcacg aaaccctccc gagcagaatg ctggcgttat tgccgccaaa
    19261 ggcgaaggaa ttggacagga tcaccggacg tgccagcggc tgcggttttt cgataatgcc
    19321 acacggtggc agcgtgggat cgcgcggcga caggctgaag tcctgagcgg gcagcggtaa
    19381 atcacgctgt aaaatcaaca tactaattgc cgcctcggta atgcccgctg cgcccagcgt
    19441 gtgtccggtc aaatgtttgg tggagctgca cggtacgcgt tcgccaaaca gggcgttaac
    19501 caccattgat tcaatctgat cgttgagttg tgtggcggtg ccgtgcaggt tgatatagcc
    19561 gacgtcatcc ggcgtaagct gcgcatcggt cagcgcctga ttaatagcgc ggatcgctcc
    19621 ttcgccctgc ggatgcgggg ctgaaatatg gtaagcgtcg ctcgattcac caacgcccag
    19681 caacgcgatg ggctgcggtt cccgggtcag cagcattagc cccgcccctt caccaatggt
    19741 gatacctgcg cggtcacgac caaacggctg acataaagtt ggcgacagcg attcgaggct
    19801 gtgaaaaccg ttaatcggca tccggctcag ggtgtctgcg ccacccacaa tggcggcatc
    19861 gaccagcccc gcttcgataa gcctgcgccc gctgattatc gctctggcgc tggaagagca
    19921 ggcggttgaa agggtatagg ccgggccgtc gagcgccagc cagtggctga gaaaacgcga
    19981 cggatcgccg agttcctgtt gtgcatagtg ccacgtggtg ctcggctgcc cgtgggtgtg
    20041 caggttaacg tgcgtgtcgc cttcatgcag gccggaggtg ctggtgccaa gcacaatcgc
    20101 gatgcgctcg cagccatatt tcgcgatagc gtcatcaacc tgcggctgga gctgcgccag
    20161 cgccgccagc aggatctgat tgttgcgcga gcgatgcgca gcgaattttt ccgggataag
    20221 cggaagctcg ccatccacgc cagccaggac cgcctgcgga tgcccctgca accagcctgc
    20281 gcgtgggcgc atgccggggg cgacaccgcg agccaggttg gctgcgattt cgtcaaggtt
    20341 atttcccagg gcgttgatca tgccgacggc ggaaatataa atcatatcag tcacctaagt
    20401 attgaatggt gatgtggtat ttaaagacat gctgctcaat tctgattggc acgcgttttc
    20461 cctggcgatt cagataggtg atttccgtga ccagtttgcc gctggcgtta cgcagctcgc
    20521 gtttgtcacc gttgtcgcga agtgtccatc ctgcgggaag ttgcggttgc caggcgctaa
    20581 tcggccagtg gctgagcatc acgtcagcca gtacctgact tgcgggcggt aactgtggga
    20641 cgacgatgga ttgctcggcg cgtagccctt gcgcatcgta ggtcaccaga aacaggcgaa
    20701 tgccgaccga cgacagcccg gcaagggtga ttttctgatc gtcggcgtta agcatcacta
    20761 acagcgactg ggttttgccg ttgaagctgc cggtcaacag ttgctgcgaa ttgactgcgg
    20821 gtgaaatccc tggtgccggc agcgtgatgc gcgtgccagg ttgcagccac gcctgcgggc
    20881 ggccttcctg ttctggttgc gagtggctac agccggtgag gataagcgta gcggtcaggg
    20941 cgaacgctcg ccagaatgtg gatttgatca tttttttgtt ctctttttat cgggcatagc
    21001 cagcggcgaa agcaggaagg cggtgaaaat accgctcacc agcacaatgc caaaactgct
    21061 gatggcctgg gtggcgctga ataccaacat tcccagcgtc agcaaggtgg tgagcattgc
    21121 cagcgcgatc gccagtagcg aagttaacgg tgtaccgcgc ggattactga aaaacagcgt
    21181 gtagttgata ccgatgccaa gcaccagcac cagcgccagc agcgaaaaga gattcaccgc
    21241 ctgcccgctc atcgccagca ccgccagacc acagcccagc gaaagcaccg aaggcacaag
    21301 gctgataagc cctttgcgcc agccgagacg ggccactgcg ccgcaggcaa tcactgccag
    21361 cgccaccagc aacaagccgg ttaagacgta gcggtaaagt gcgaacaatt catcaaaggt
    21421 gcttttgcga tcaacccagg caatgccgca agggtaatat gtggcgattt cctgcaacaa
    21481 tgcgctactt ttaacccctt caactggcac cagtacaccg ctttcgccgt tttccagcgt
    21541 cagccacagc agacgccagc cttcactggc ggggcttgcc agccattcat cgaccttcac
    21601 cggcatggcg ttgagatctg gtttcaccgc cgtcagccct gcattttgca gtgcttttgt
    21661 taccgtcggg gcagccgttt tcagcaggtc taagtcttcc tcctgccgcg ccagggagtt
    21721 cagcggaata gtgcggtagt tgctgataag cccctctttt ttcgcatact caagtgaggc
    21781 ggtatatttc tccagtcgcc gcaatgtttg ctgtggggag tcgccgtaaa ctacaaacca
    21841 tttttgatcg acgctttgtc cggtcagggc ggtaatggct ttctcctgcg ccagaatgtg
    21901 ctgcggcagc gcctgtaact gcgagatatc gtcatcgacg cgcagcattg acatccccgc
    21961 cagcgaaaac agcgccagcg caacgggcag accccgcgac agttttttat tgcgccgcca
    22021 cgctgccagc cagcgtagca tcagcgccat cgccggaacc gggcgcaccg gcagaccacg
    22081 gcacagccac ggatgccaga acaggacggt cagacaagag gcgctcaacc cgacggcggc
    22141 aaaaatcgcc atctggcgaa tgccggggaa gggggcgagc atcataatca gatatgccgc
    22201 cacggttgtg agcagcgcca gcagcagggc attgcgtact ttcgccaggc tttgccacgg
    22261 cgaaacgtcg ttgccgtgca ccatccgctc ggtgagataa tagagcgtgt agtcagcgga
    22321 aatgccgata acgctcatgc tcatcaccag cgtcatcagg tgtaattcac cgaaaatcaa
    22381 caatgtggcg gccgttcccg ccagcgcgcc gatgccgatg gaaatcacgc aaagcagcaa
    22441 cgggcgcaaa gagcggaaca ccgccacaat cagcaatatc acccccagca gcgttgccac
    22501 gcccagggtg gagatatcct gcttcgcctg ttggctggcg taatcgctgt aaaacaccgt
    22561 gccgcgtgag agcaactgcg cctgcgggta acgcgttttc agagcctttt ccagcgtatt
    22621 cagggtcgtg atgagctggt gggtttgctg catatcaaac gacgatcccg ccagttcgcc
    22681 gtgcagcaga taccagtaat tgccctgggg atcctgcgtc accagccagc cgtccattag
    22741 tcgtaaacgc tggccatttt tcgccatcgc cagttgcgag ccgcgcatta acatcagcgg
    22801 atcgttttgc agctccttgc cgcttacccc ggagaacgca gaataaagct gggagaggat
    22861 ccacttcgcc tgcgcttcgc cgccattttg caggcgggcg cgggtgtcgg ggtcaatcag
    22921 gccgttgcgg tgctgccaga aaaacgctcc ccacgcctgc tggctggcgg catccattgg
    22981 ccctttaatg tcgccgagcg cagcggattt ttgcagcagc gtcagccact cctgagccac
    23041 cctgggatta gcctctttac cggggctgac cagccacacc agttggcggt ccagacgctg
    23101 cataaagccg tcattcagcg ccggaggaat atcgcccatc gcctgtttgg gtaacatagc
    23161 cagcacgcta ctgttcagtc gtgattgtgg cagcaggatc agcaacgccg ccgccatgac
    23221 caggcagact agcccccata acagcgcggg gcgtttactg ggtggcaaag cgttggcgtt
    23281 cgtcatcggt cagttgcgct ggtgtcagtt gatgttgggt aagagcgata tcggtgtgat
    23341 cgccctgttt atcattaagc tgaatgctct ccagataggt ttgcccggcg agatcgatgg
    23401 tgttgaaaat tttgtccagc ggcgtggtgg tcggcgtcag gcgcagcgtc cagcggcctt
    23461 cgcctttgtc ggcaaattcg acgcggaagt tttgttccag cactttgcga tcggcctgga
    23521 acagcgcgcg cagcaggtgg ttaaactgga acatctgcgg gttgttttct gccgtgatga
    23581 tttgcggcgg ctgaccgttg atcacctgca ccatgcgttt atcgtccagt aatagttgca
    23641 tggggaacgg tgaggtttga tcccacaata acccctggtc gcgggcgatc aacatctgac
    23701 cctgagatcg cagcggctgc ggcagatctt taatcgtccg ggtttgatcg aaatgggcgc
    23761 ggatcaccgg ttgttcggta aagcgttgct gaagatcgtc cagggtcagg gcgctcacaa
    23821 acgggctaat cagcagcacc agcagcggta aaaatttcat ggtttgactc ccatgcgttc
    23881 aaacagaata tcggggctga caaagcacag ttcacggctt tgctcctcta ctgcgacctg
    23941 aatggtgtat ccggtggtgg cgcgtttgcc cgtttcggca tcgaaaatct gatagccaat
    24001 ccgcagacgg ttttcgaact cttcgatatg cgcacgcacg cggatgcgtt gctcaaaggt
    24061 gagggcgtgg cgatatttca cccgcgcatc gaccactggc cacaaataac ctgactcttt
    24121 catctgccga tagccgtaat tgaactggtt gagcagcgcc tcgcgggcca cttcaaagta
    24181 gcgaaaatag ttaccgtgcc aggccacgcc catcatatcg acgtcgtgaa agggaatggt
    24241 cagctcgact tcagcggtaa atcggggatc gttaagcacc ctttactcct tgtcctgaat
    24301 ttccggcagt tgccagaaat cgaaaaaatt aaaccagtcg aggggcgact ggagcgcgta
    24361 atgttccaga cgcgcggcgt agtggtcgat agcgttttgc agcgcctgtt ggcgttcgcc
    24421 acgcggcaac agcagcgggt cggcaaaggt ttcgcagtga atatgcagct tgccgcgctg
    24481 gcgcagggcg aaaatcagat tcaccgggca gcgcaaaata gacgcgagaa taaacggccc
    24541 ctgtgggaaa ggcgcaggct ggcccataaa cggactccag cagacgcgcc agtcgccgcc
    24601 gcgttgcggg gtgacggcga tgcggtcacc gacaatcgcc acccattcgc cgttatccag
    24661 cttctctttc agcaggatgg cggtttccgg gccgatatct gttaccggca tcaggttaat
    24721 gcctgcctga ggagccatct cctgcattat ctgtttaaag cgttgggcgt tttcgctaaa
    24781 caccagcgcg ttaatggttt tgtagccctg aatttttgcc agcgcccggc acacttccac
    24841 atcgccaaga tgcgaggcca gcagcaattt gccgcgcgga tcgcggacgt caagcgccgc
    24901 ttctgcgcct ggcgcaaaca gcacatcgtg ccccagttgt agctcgccgc gccagctggc
    24961 gattttgtcg agcatggcat taccgaaacg tagaaagtgc tgatagctgt tgagttttgc
    25021 cgcctgtggt tgatgctgtc gtacacgggc gagccagtct tgcgacgctt tgcgcgcacg
    25081 agcagcagtg agccagtaga cgcccaccac cgggtaaagc agcgcgctaa acgccgttct
    25141 tcccagcaga cgccagacca gcagcatcag gcgcattccc cataatcctt tcacttcact
    25201 ctgccgcgcc cagtggctgg aagagcggcg catcagtagt gaaggaatac gcggcaacat
    25261 gccgaaaaac agacgcgtgt gcatgagcga gatgcggacg ttatctttca gggcatcaaa
    25321 atgcgaaagc ccgtcctgcg gataagtgac gcgggttggc acgaaataac tggtgtttcc
    25381 ctgccagtag aggcgcacca tcacttcagt gtcgaaatcc atccgcttgc cgatggtggc
    25441 gtattttgcc agttgcagcg ttggcgcaac cggataaacg cgaaagccgc acatactgtc
    25501 tttcagttgc agggagagcg tttcgatcca tacccagacg tgggtgaccc agcgcccgta
    25561 aagccgtgag cgggggatgg agtcatcgta aattggctgg ccggagatca gcgccacagg
    25621 gtgttgttca gcgagtgcca gcagtttggg gatatcttca atcgcatgtt gaccgtcagc
    25681 atccacctgc accgcatggc tgaagcccgc gtctgccgcc gcctgtaagc cgcgcattac
    25741 cgccgcgcct ttgcctgtgt tttctgccag gcgaattaag gtcacgccag gctgttcggc
    25801 aaccagatta tccagttgct gttgcgtggc ggcatcgctg ccgtcatcca ccacaataca
    25861 gggcagatta aatggcttaa gacgcgccag cacgcccggc atcatcgcgc cgtggttgta
    25921 gcaggggatc aacacgcagg gagaaaagtt taccgacata ggcggatctt cccgctactg
    25981 gcggtgtggc gggcgtcacc gtcgtgacgc tgatagcaga aagtgagtac ctgacttgtt
    26041 tcctgccatg ttaattgcag ggtaacagtc gtctcaggta taagtggcgc cagaaattta
    26101 acattctgga tactacgaaa acgccagccc ggagcaagca gcgtggtagc ataatgcatt
    26161 acccagtcca actgggccac gcctggtaac agaggctgta cggcaaaatg accattaaac
    26221 cagtagagca tcgggtcaag gtatagaacg atttccatct cctgcggttg aaccagatgg
    26281 cgctcaattt catggcgtat catgaaataa ctcctgtaat tgcgcataga cacgcttgtt
    26341 catactgttt actggtattt catcaataat gcgccagtaa cgaggtactg caacaggttc
    26401 aagccatggt aatagtgctt tgcgccagga caactccaga gttttccctc cggagcattg
    26461 ccatatttga cgggcctctt tatttggtac aaccacgaca ccgatagcct ggcggccatt
    26521 gcgtattaat ggtattgcag cgacatccat aataccgtca agtgccagaa gtcgttgttc
    26581 gatttcaacc agtgatatcc gcttctcctc tatttttacc actcgaccac gtcgtccgat
    26641 caggtggaac tgaccgtttt cattgaattg caacatatcg tcaagcacca atccttcagc
    26701 agggattagt ggcgaaaaga cacgaacccc ctcactttcc tgtgaaagat gaacatcaga
    26761 aaaagtaaac caggggatat tatcctgttg acggtaacgc catgcgagaa tacctgtttc
    26821 ggtactacca tagatttcat cgggccatat attcagccag gtggaagttt tctctacatc
    26881 ctgccatggt agcataccgc cggcagagag aatcatttcc actgggggag gcgtcagatg
    26941 aaggtcgagt cgcttaagga acgccgggct gctgacaaag gcatagcggt aggtatggct
    27001 taatgcggca agttgctcag aataccagag cattgcggca tgcaatggca ggccaagtgc
    27061 catgggtaaa aaaatacgga aagttagccc atacaggtgc tgaggcatca cggatgctac
    27121 aaaacgacag cccagtagtc ggtctgcaaa gtgagttgcc agcatgtttg cttcttgatc
    27181 aaggctgata acaggttttg caatttgttt tggttgtccg gttgaacccg atgtgaaaag
    27241 ttcaataaac gcgtcagaag caatatcatc aaaggctatc gtgctggtcg tcatctggtg
    27301 agatgtgctt acaacaaact gtggaccgtt ccagcccagc cttttatcac tcaggacgca
    27361 atcaaacaag gagcgttgta catttaacaa agcggcgcga ttatggcccg ggattaccgg
    27421 gattttacct gagtgtagcg tggccaacaa cgctacaata aaaagatagc tgttttcaaa
    27481 acaaagtgcc cagcgattgc ctggctggtc gcgtaaatgg tcgatcaaaa gcgctacatc
    27541 atgccgcaga tgccccagtg tccaggtata ttccccaagc caggcaacag gagttgttac
    27601 agggcgagag gctgtcaacc actgtgaaag tgagagagtc tgattcatgg tgttttccgt
    27661 ttgatcatct tacgacgtat cagccattcc cctgcagtca ggaaacctat cagaaggtaa
    27721 gcaatgatac cgttccaggc tgtccacaag ggcatgttgc catacaaggt ggtaaataac
    27781 gcaatactgc cattgaaaat aaaaaacagg caccagacct gcgttacgcg gcgggtatat
    27841 cgcacggcct tttccgggag gtctggctcc tgcaaacgtg ccagccgctc aactatcggc
    27901 attgtcgacc gcagcgaacc gccaaaaacc gccagcatga ctgcgttaac cactaccggg
    27961 taaaacagca gtaactggta cgttttcaac agaaaactgg agacacacaa tacaatgccc
    28021 acaacagcaa cgatttttgt gacgacctgg agcggccccg tctggcgacg gggctggcgc
    28081 aggcgcaata aaagcaacag cgccatcagc ggcaacagcc agtgcaggct gttatgcacc
    28141 aaacctagcc agattaaaaa aggccagacg agcagcaata atccggtcag caacttaatc
    28201 atcggtagcg agcgaatgcc cgacatcaaa ttagccttct tgtagcaggc gttctacagc
    28261 ctcgacaaca tcctggactg tacgcactgc tttaaactct tccggcttga ttttcttacc
    28321 ggttttcttt tgcaggtgca caatcatgtc aacggcgtcg atgctgtcca gttccagatc
    28381 ttcgtacagt cgcgcttcag gtttgatgtc ctgcggatcg atttcaaaca gcttaaccag
    28441 cagggctgag acttcctgat aaatggtttg ttggtctgtc atgatggttt tcctcaggca
    28501 cgttgcgcag caatgaaaga ggccagagtg gcgacggaga aaaagtgctg acgcatttct
    28561 tcgctttccg cagagagcac gatgccgtac tcatttttta ctgccagtcc cagttccagt
    28621 gcatcgatag agtccagccc taaaccgtcg ccaaacagtg gcgcattgat atcaatatca
    28681 tcaggagtca agtcgtccag attgagggtg ctgatgatga ggtttttaat ttccagataa
    28741 agcgcttgca ttatttattc ctgatagagg taaacaacct tgctgtaatt gaagcaaaag
    28801 atgccggttt aactgtcttg cagccagcgt cggctcttgt gaagttgcat cgtaaaattt
    28861 attgatttct acccgcccac ggacttcaac cgtaaaaaac ggcctggcag gcgggacgtc
    28921 ataccactta ctttgtttat ccagcagatg ctcgctacag ccgatcgtca cgatgcgtaa
    28981 atcgctggtg cagcgcacgg caatattcgc tgcaccgcgc tgtaacgtca ttttttcacc
    29041 gggcttcgtt cgtgtgcctt cggggaaaat caggatagtg tcaccctgtg ccagtcgctg
    29101 ttggcagcgg ggtaacaggg tttctgcttc gctgttgatt aaataatctg ccgctcgcac
    29161 gacgccgcca agaaaaggat tttttaacag cgcgcttttc accaggcagt cagtttctgg
    29221 catgactgat gccagcagca cgtaatcgat cagcgtggga tgattggcta cgacgaggca
    29281 accgcgttcc tgacgtaaaa tttcagcgcc atcaatacga taatccagca cgccaagccc
    29341 tttcgcgaca gttaaaaaca ggcgaaaact ggcggcaatg ctgcgacgtg ccaaacgacg
    29401 gcggcgggaa gtatcccaca ccaaaactaa caatatgtta aaccacacga ctgaaagcag
    29461 caggccgccg agaccaaaca ggacaaaaca taacccggtc atgaccagtc gccatgccca
    29521 tcccaggcgc gacatcagct tactcatctg cggctccagc gccattgcac gcgctcgcca
    29581 ggcagtgaaa aagcatctgc attttgtaaa tagtgctgta aaaacaagat gctttgcggc
    29641 agtgttgttt catttaccgc aatggctggc tgcgtttcac attgccagtc atcgccagct
    29701 tcaatcacca gcgcgacggc gtacggccag gtaggcattt ctgcgggcag ttgcgggtga
    29761 taaaactcag gtagaaagcc atcaaaatcg accatcaaca cgcgctggta tcccgcttgt
    29821 agcaggctaa gcacttcaca caaaccttgc tggaaactgt cgcgaccagc cgaaagcgat
    29881 gacgaaacga tcggctgttt ggccacaatg gtcaggttac ccacggaaga gttgtgtacg
    29941 gaaagcgcga agtctgtcgg agaaagcgcc tgttcggttg ccagtgcatg aacaatgcgg
    30001 taattgcgct ccagttcgcc atgacgactg gtatacaaaa cagcgtctgg ctgatggcgg
    30061 cgtagcattg tcagaccgca ttcaacagcc agtttgctgc ctgaactgag acgacgagcg
    30121 gtcatcattg gcagttcact taattttgcc aacggcgcgg cagggtcaat cgcccacggc
    30181 tggcgcgacc atgcttgcca ttgctgcaca tcgctaagtc caggcgccag tgcctgccag
    30241 ttggtaatat ttaatgcaaa tttcatcaac gcgcgcccgc ggtaaaccaa aattattaag
    30301 tgacgattat ttcttttggc atatcaaaag tgtatgccca acgcctaaat tatcgtgacg
    30361 ctctgctacc tggaatcctg ctttattcag atagtcgtaa aatttctctg cactatagaa
    30421 acggctgtta ccgtttgcca tgcaggtaaa ataaagtgag gatgcattca ggctaaacga
    30481 tgtcgcttca aaccgctgtg catcccagaa caactccatg atgcaaagtt ttgcgccagg
    30541 cttcattacg ctggcaattt tgctgagtat gctaattatt tgttcgggtg aaaaacagtc
    30601 taaaaattgg ctcatccacc agatatcagc ctcgcctggt aatggggcat cgcttagcat
    30661 atccaccgca tggaaatcta tgcgatcaga aaaacctgca ttcgcgatat tttctttcgc
    30721 cagtacgatt tgttgtggca gatctaataa cgtaacggcg atgttttcat tgaatttgca
    30781 gcagcgcaaa gcccacttac ccgtattgcc acccacatcg tacaaggttg tggggttgtt
    30841 ggcgaataca taaggcagtg cggcgttaaa tgcgccatct gaatagtaat gatcaaacgc
    30901 aaaccagcta tcacgtgcgg cgtctggtaa ttgcgaaagt gccggatata tcgtcggcca
    30961 gtcgccaaat acttttaacc cggaaggttt tccttcattg agtgaatcgg caaggaagaa
    31021 gagcccctgg taacagacat cctgggtgaa atccatattc acgcgagtca tagtgtcatg
    31081 caggaggtaa tgaccgattt tcgccaggta ataaattccc tctttacagg taataattcg
    31141 cccgcttaag cccatatcca gtaacacacc cacggcatat tcattcagga ctgtgtgctc
    31201 agtaatcgca ttaaggggag ctccctgttt accctgttga tccaaataat ctaatattcc
    31261 tgcattgcgc aggcacaaag ctgcctggaa aagcatagga gcaaaggcaa tccgctgtgc
    31321 ttcggtgatg gcatccagtg cgctgagcgt gtcctgttcg tacatatata accattctta
    31381 tagagaaagt taaatactat cgggccattc cagcccgata tcagcatcac tataacgttg
    31441 ctcctgtaga taagttaacc tgtatatctt tatctaggtt acgcacccag cggttatagt
    31501 ttttatgaat ttttccatca gaagcctgca gattcaggct actgtcatat ttgatgttat
    31561 agtaggtagc tgtatatgta atacgaacct ctgcaacgtg atttcgtgtt tgatagcgag
    31621 ctttaataac tccaggggac acttgcgtca taatccactt gcgctgcgca cctgctttca
    31681 aaatggcatt tttaacctgc tcctgagtat gacccacact gacagtggtg cttatctgtt
    31741 gcactggggc cgtacgggca caggcactca acatactgat aaaaattgtg atggcaaacc
    31801 attttatata ac
//
LOCUS       DAPPNJ010000032        31479 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000032 DAPPNJ010000000
VERSION     DAPPNJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.032"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            765..1637
                     /locus_tag="RPM73_001179"
     CDS             765..1637
                     /locus_tag="RPM73_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDX6519560.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2009..5128
                     /gene="ag43"
                     /locus_tag="RPM73_001180"
     CDS             2009..5128
                     /gene="ag43"
                     /locus_tag="RPM73_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDX6519561.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVLSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTIILRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASTD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            5249..7765
                     /locus_tag="RPM73_001181"
     CDS             5249..7765
                     /locus_tag="RPM73_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519562.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            7841..8296
                     /locus_tag="RPM73_001182"
     CDS             7841..8296
                     /locus_tag="RPM73_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519563.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            8375..8608
                     /locus_tag="RPM73_001183"
     CDS             8375..8608
                     /locus_tag="RPM73_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX6519564.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            8708..9526
                     /locus_tag="RPM73_001184"
     CDS             8708..9526
                     /locus_tag="RPM73_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDX6519565.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            9608..10087
                     /locus_tag="RPM73_001185"
     CDS             9608..10087
                     /locus_tag="RPM73_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX6519566.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            10099..10575
                     /locus_tag="RPM73_001186"
     CDS             10099..10575
                     /locus_tag="RPM73_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDX6519567.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            10638..10859
                     /locus_tag="RPM73_001187"
     CDS             10638..10859
                     /locus_tag="RPM73_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX6519568.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            11022..11390
                     /locus_tag="RPM73_001188"
     CDS             11022..11390
                     /locus_tag="RPM73_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDX6519569.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            11480..11854
                     /locus_tag="RPM73_001189"
     CDS             11480..11854
                     /locus_tag="RPM73_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDX6519570.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            11851..12339
                     /locus_tag="RPM73_001190"
     CDS             11851..12339
                     /locus_tag="RPM73_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519571.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            12351..12548
                     /locus_tag="RPM73_001191"
     CDS             12351..12548
                     /locus_tag="RPM73_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519572.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            12645..12932
                     /locus_tag="RPM73_001192"
                     /pseudo
     CDS             12645..12932
                     /locus_tag="RPM73_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            13004..14008
                     /locus_tag="RPM73_001193"
     CDS             13004..14008
                     /locus_tag="RPM73_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HDX6519573.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            14290..14574
                     /locus_tag="RPM73_001194"
                     /pseudo
     CDS             14290..14574
                     /locus_tag="RPM73_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(14855..15391)
                     /gene="yghD"
                     /locus_tag="RPM73_001195"
     CDS             complement(14855..15391)
                     /gene="yghD"
                     /locus_tag="RPM73_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDX6519574.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMVARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(15393..16571)
                     /gene="gspL"
                     /locus_tag="RPM73_001196"
     CDS             complement(15393..16571)
                     /gene="gspL"
                     /locus_tag="RPM73_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDX6519575.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMVLKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(16568..17545)
                     /gene="gspK"
                     /locus_tag="RPM73_001197"
     CDS             complement(16568..17545)
                     /gene="gspK"
                     /locus_tag="RPM73_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDX6519576.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLRLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(17542..18147)
                     /gene="gspJ"
                     /locus_tag="RPM73_001198"
     CDS             complement(17542..18147)
                     /gene="gspJ"
                     /locus_tag="RPM73_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDX6519577.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNCVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(18144..18515)
                     /gene="gspI"
                     /locus_tag="RPM73_001199"
     CDS             complement(18144..18515)
                     /gene="gspI"
                     /locus_tag="RPM73_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDX6519578.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(18512..19075)
                     /gene="gspH"
                     /locus_tag="RPM73_001200"
     CDS             complement(18512..19075)
                     /gene="gspH"
                     /locus_tag="RPM73_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDX6519579.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(19079..19534)
                     /gene="gspG"
                     /locus_tag="RPM73_001201"
     CDS             complement(19079..19534)
                     /gene="gspG"
                     /locus_tag="RPM73_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDX6519580.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(19551..20774)
                     /gene="gspF"
                     /locus_tag="RPM73_001202"
     CDS             complement(19551..20774)
                     /gene="gspF"
                     /locus_tag="RPM73_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDX6519581.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILIASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(20774..22267)
                     /gene="gspE"
                     /locus_tag="RPM73_001203"
     CDS             complement(20774..22267)
                     /gene="gspE"
                     /locus_tag="RPM73_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDX6519582.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQAAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(22267..24327)
                     /gene="gspD"
                     /locus_tag="RPM73_001204"
     CDS             complement(22267..24327)
                     /gene="gspD"
                     /locus_tag="RPM73_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDX6519583.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGQGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(24357..25316)
                     /gene="gspC"
                     /locus_tag="RPM73_001205"
     CDS             complement(24357..25316)
                     /gene="gspC"
                     /locus_tag="RPM73_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDX6519584.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(25334..25744)
                     /gene="gspS2"
                     /locus_tag="RPM73_001206"
     CDS             complement(25334..25744)
                     /gene="gspS2"
                     /locus_tag="RPM73_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDX6519585.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCTDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(25810..26619)
                     /gene="pppA"
                     /locus_tag="RPM73_001207"
     CDS             complement(25810..26619)
                     /gene="pppA"
                     /locus_tag="RPM73_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDX6519586.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(26946..>31479)
                     /gene="sslE"
                     /locus_tag="RPM73_001208"
     CDS             complement(26946..>31479)
                     /gene="sslE"
                     /locus_tag="RPM73_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDX6519587.1"
                     /translation="LAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPN
                     PEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESS
                     DGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKS
                     NALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNA
                     ATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGY
                     GVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEV
                     RGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQV
                     VNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGV
                     DTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKR
                     AWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRC
                     PNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTV
                     YFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDP
                     YAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAG
                     LSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQ
                     VENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKE
                     CTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRL
                     YQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFL
                     NCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLG
                     RSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVT
                     IKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIY
                     IKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNA
                     SNYTGGLKQFANDLDTFASSMNDFYGRNGESGNHRMFTYEALTGHKHRFTNDVQISIG
                     DAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLAL
                     YMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIK
                     QWYPEGNLPKFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTL
                     MLCASWVAQTDLSAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTLASLGLPKPQQ
                     GPETINQVTEHKMSAE"
BASE COUNT         7341 a         8658 c         8099 g         7381 t
ORIGIN      
        1 aaatgcacca ttttttcttc aaatacttct gtataatcgc cagccaatga tgagaatgga
       61 ttaaaataac acttcttcga caaacacaaa aacagccttc attttacatc atcatccagt
      121 ttcagttaac cccggacgag cgacagcgtt tcagtacagg aattaaaagc cagcttttgc
      181 tcagtaaaaa cacactcaac taataatatc tcagcagata ttatcctgac caggacacct
      241 cacattactg aattctcttc cgacagtatc actgcacaaa taaaaaagaa cgggataaat
      301 attcagaccg gaagaatggt gtcacaaaag catttaatca ttacgcatga ccaggaaggg
      361 aaacacttaa ccacatggta tcaacagata tcattacgtg tgcactctta taaaaaacac
      421 acagaagaag taagataatt ataaacgtaa atttaatatc tcccactgac atctccccca
      481 caatacactg aaaaatactc acagcagtaa acacagacac acctgataat catgcagccg
      541 ataaatattt tcccctttac ggagaaatca gcctgctgtt taacaataac ggcgtatttt
      601 catggaagga gatcacccag taacggataa gtcctgatct ccttcatgtt aatacgcaac
      661 aatcccatcg attcaaaaca atttcagaca gatattatct ccgtgaatcc cccgccacct
      721 ttccggtgcg cggggttttg tcttttttca ccgggaatac atgtatgaat ccgtctgatg
      781 ccattgaggc aattgaaaaa ccgctctcct ccctgcctta ctcactttcc cgtcacatcc
      841 tggaacatct gcgcaaactc accagtcacg aacccgtgat tggcattatg ggtaaaagcg
      901 gggccggtaa atcctcactc tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg
      961 atgttcacgc cggcacccgg gaagtgcggc gcttccgtct gagtggccac ggtcacagca
     1021 tggttatcac tgacctgccc ggggtgggcg agagccggga cagggatgca gagtatgaag
     1081 ccctgtaccg tgacattctg cccgaactgg acctggtgct gtggctgatt aaagccgatg
     1141 accgcgccct gtctgtggat gagtatttct ggcgacacat cctgcaccgg ggacatcagc
     1201 aggtgctgtt tgtggtgacg caggccgaca aaacggagcc ctgccatgaa tgggatatgg
     1261 ccggtattca gccttctccc gcacaggcac agaatattcg cgaaaaaacg gaggcggtat
     1321 tccgtctgtt ccggcccgta catccggttg tggccgtatc ggcccgcacc ggctgggaac
     1381 tggatacgct ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc agtcccctga
     1441 tgacccgcct gcaggacgag ctgcgcacgg aatctgtccg cgctcaggcc cgtgaacagt
     1501 ttaccggtgc ggtggaccgg atatttgaca cggcggagag cgtctgtgtt gcctctgttg
     1561 cccgtacggt tctgcgtgcc gtccgtgaca cggtggtctc tgttgcccgc gctgtatgga
     1621 actggatttt cttctgaaca ctctgccggg ggaaatgcct gattacattg cgttaaccaa
     1681 ctgaaaacat gccccccccc cctcataccc acctctttct cttgcgtgac ttccctgccg
     1741 ttaatagaat aaaacgatcg ataaaacagg tatcgatcgt tactatcgat cgtttatagt
     1801 ggttaatggt ctgcgaaaag gcagctgaat ctcttcatca tgcagaacgg aattgcacac
     1861 aacggactga tacttctctg gcggatgaga gaggggaagc atttatgtcc gggaaaaacc
     1921 actttatctg agcatgctgt tttgtacctg ccggtatcca catttgtggg taccggcttt
     1981 tttattcacc ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct
     2041 ggtatggaat cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg
     2101 aaaacgcgct ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact
     2161 ggctgctgac aaggttgtac aggcgggaga aaccgtgaac gacggaacac tgacaaatca
     2221 tgacaaccag attgtcctcg gtacggccaa cggaatgacc atcagtaccg gtctggagta
     2281 tgggccggat aacgaggcca ataccggcgg acaatggata caaaatggcg gtatcgccaa
     2341 caacactact gtcaccggtg gtggtcttca gagagtgaat gccggaggaa gcgtttcaga
     2401 cacggttatc agtgccggag gcggacagag ccttcagggg caggcagtga acaccactct
     2461 gaacggcggt gagcagtggg tacatgaagg cgggattgca acgggtaccg tcattaatga
     2521 gaagggctgg caggccatca aatccggcgc agtggcaacc gacacggttg tgaataccgg
     2581 cgcggaaggg ggaccggatg cggaaaatgg tgataccggg cagaccgtct acggagatgc
     2641 cgtacgcacc accatcaata aaaatggtcg tcagattgtg gctgctgaag gaacggcaaa
     2701 taccactgtg gtttatgccg gcggcgacca gactgtacat ggtcacgcac tggataccac
     2761 gctgaatggg gggtaccagt atgtgcacaa cggcggtacg gcgtctggca ctgttgtaaa
     2821 cagtgacggc tggcagattg tcaaaaacgg gggtgtggcc gggaatacca ccgttaatca
     2881 gaagggcaga ctgcaggtgg acgccggtgg tacagccacg aatgtcaccc tgaagcaggg
     2941 cggcgcactg gttaccagta cggctgcaac cgttaccggc ataaaccgcc tgggagcatt
     3001 ctctgttgtg gagggtaaag ctgataatgt cgtactggaa aatggcggac gcctggatgt
     3061 gctgaccgga cacacagcca ctaatacccg cgtggatgat ggcggaacgc tggatgtccg
     3121 caacggtggc accgccacca ccgtatccat gggaaatggc ggtgtactgc tggccgattc
     3181 cggtgccgct gtcagtggta cccggagcga cggaaaggca ttcagtatcg gaggtggtca
     3241 ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc
     3301 cacggatacc acggtaaatg gcggactgtt caccgccagg ggcggcacac tggcgggcac
     3361 caccacgctg aataacggcg ccatacttac cctttccggg aagacggtga acaacgatac
     3421 cctgaccatc cgtgaaggcg atgcactcct gcagggaggc tctctcaccg gtaacggcag
     3481 cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca cccagaaagc
     3541 cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca cggatgtcat
     3601 tgctcagcgc ggtacagccc tgaagctgac cggcagcact gtgctgaacg gtgccattga
     3661 ccccacgaat gtcactctcg cctccggtgc cacctggaat atccccgata acgccacggt
     3721 gctgtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct ccacccgcac
     3781 agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga atggcaccat
     3841 catcctgcgt gtacgcccgg atatggcaca gaacaatgct gacagactgg tcattgacgg
     3901 tggcagggca accggaaaaa ccatcctgaa cctggtgaac gccggcaaca gtgcgtcggg
     3961 gctggcgacc agcggtaagg gtattcaggt ggtggaagcc attaacggtg ccaccacgga
     4021 ggaaggggcc tttgtccagg ggaataagct gcaggccggt gcctttaact actccctcaa
     4081 ccgggacagt gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg cagaagtccc
     4141 cctgtatgcc tccatgctga cacaggcaat ggactatgac cggattctgg caggctcccg
     4201 cagccatcag accggtgtaa gcggtgaaaa taacagcgtc cgtctcagca ttcagggcgg
     4261 tcatctcggt cacgataaca acggcggtat tgcccgtgga gccacgccgg aaagcagcgg
     4321 cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggttg ccggtatgtc
     4381 tgtgaccgcg ggggtatatg gtgctgctgg ccattcttcc gttgatgtta aggatgatga
     4441 cggctcccgt gccggcacgg tccgggatga tgccggcagc ctgggcggat acctgaatct
     4501 gacacataca tcctccggcc tgtgggctga cattgtggcc cagggaaccc gccacagcat
     4561 gaaagcgtca acggacaata acgacttccg cgcccggggc tggggctggc tgggttcact
     4621 ggaaaccggt ctgcccttca gtatcactga caacctgatg ctggagccac aactgcagta
     4681 tacctggcag ggactttccc tggatgacgg taaggacaac gccggttatg tgaagttcgg
     4741 gcatggcagt gcacaacatg tgcgtgccgg tttccgtctg ggcagccaca acgatatgac
     4801 ctttggcgaa ggcacctcat cccgtgcccc cctgcgtgac agtgcaaaac acagtgtgag
     4861 tgaattaccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca gctcccgggg
     4921 agatatgcgt gtggggactt ccactgcagg cagcgggatg acgttctctc cctcacagaa
     4981 tggcacatca ctggacctgc aggccggact ggaagcccgt gtccgggaaa atatcaccct
     5041 gggcgttcag gccggttatg cccacagcgt cagcggcagc agcgctgaag ggtataacgg
     5101 tcaggccaca ctgaatgtga ccttctgaca gaaccatcgc ctctctgtgg tcccggtcat
     5161 catgaccggg acccggaccg gcgcaacgga tcttcaacgc cacattcgct ggcattaaca
     5221 ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc tgatcctgct
     5281 gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaatcagca cggcttctgc
     5341 gctggcaggg ctcatattgc tgtgtctgtt cggcccggcc ttactggctg gctatatcac
     5401 cgaacgcata acccggttgt tccatattcg ctggctggca ggcgtatttc tgacgattgc
     5461 cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcgc tggaggctca
     5521 cacctttgac tctgtgaaat ttattctgac cactgctctc gccggtggtc tgctggctgt
     5581 tcccctgcag atcaaaaaca ttcagcagaa cgggatcaca ccagaagata tcagcaagga
     5641 aattaacggg tattactgct gtttttatac tgcctttttc cttatggcgt gttctgcatg
     5701 cgcaccattg atcgcgttac agtacgatat ttcaccgtca ctgatgtggt ggggcgggtt
     5761 gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaga tccaggcgct
     5821 gaaaaaactg acctgtgcca tcagccagac actggaagaa caaccggtgc tcaacagtaa
     5881 atcgtggctg accagtttgc aaaacgatta cagccttcct gactcactga cggagcgcat
     5941 ctggctgacg ctcatttctc aacggatttc ccggggagag ctgagggaat ttgaactggc
     6001 agacggaaac tggttactga acaatgcctg gtatgaaaga aacatggcag ggtttaacga
     6061 acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct tccggaaccg
     6121 cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg acggcggtga
     6181 ctggtatccc ttttcagaag gtcgccgttt tgtatcattc catcacgtgg atgagcttcg
     6241 tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc ataagccgga
     6301 tccggaatgg tactgctcct ctctttgtcg cgaaacagaa acactgtgtc aggaaattta
     6361 tgaacgccct tacaacagct ttatttccga tgcaacggcg aatggtctga ttctcatgaa
     6421 actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc agggacatgg
     6481 gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa acgcacggat
     6541 cctcggtgat aataatgcca gaaatggagc agacagactg gtcagcggaa cagaaatcca
     6601 gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg
     6661 acagtatcgt tacatgggaa ataatggccc catgcaactg gaagtccccc gtgatcagta
     6721 tgccggcgct gttgaaacca tgaggaataa gatccgcgaa ggtaaagtac ccggtgtaac
     6781 cgatcccgca gaagcgtccc ggctgattcg tcggggacat ctgacttata ctcaggcccg
     6841 taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg agggttcggt
     6901 tgtcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct tctggctcag
     6961 caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa aaaccttcac
     7021 ccgcacactg gctgtctacg tcacaaccca gcaactccac cggctcagtg ttgttcaggg
     7081 tatgctgaag cacattgatt tttcgactgc cagcccgact gtccgactgg ctcttcagaa
     7141 ggggaccggt gcaggaaata tcagtgccct gaacaaagta atgaagggta cgctggtgac
     7201 atctctggca ctggtagctg tcacaaccgg ccctgacatg atcaaaatgt tgcggggacg
     7261 gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttccggtg tggcaggtgg
     7321 agctgtcggg tcagtggcgg gcgggatact gttcagtcca ctgggaccat ttggtgcact
     7381 gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg tatcaggaaa
     7441 aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga ttcaggagca
     7501 ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa atctgaacga
     7561 gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg ccggtataca
     7621 acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca tcaggcaacg
     7681 ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac tggaagaagc
     7741 attacccccg gagttaccgg cataacggct attctgcgcc ttttacgctt tatcacctga
     7801 tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc tgttcaaaac
     7861 ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc gggcacagga
     7921 tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa atatttgtgc
     7981 ttaccagttc cggctggata atggcggaaa cgatgaagga tttggccccc tcaccatcac
     8041 tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg aaacggaagc
     8101 ttttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg agtgcgtgga
     8161 aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc atcgtgtcag
     8221 tctgccgtta tcggttttca atccccagga ctaccatcca ctgctgatta ccgtttccgg
     8281 aaaaaacgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgtc acaacgcgtg
     8341 gcagaggctt tttatttatc aggagaaaac cgatatgccg ggttacacag agtatgtcct
     8401 ggctgaagga tctttctcat acgggcaggc ggttgctgtc ataaccgctt accgcaatgt
     8461 gtttatccag gatgacccgg gaatgcattt ccgtcgggtt atccgcaacg cggaaggtca
     8521 gcgacgctgg cgctgccgga attctgaacc cgatgccgga aaggtgctta atacgcggct
     8581 cgccagtgac ggtcttctca gacaataaac gtcattcatt tcacccatca ggccgcgtct
     8641 tctccgggag acgtggcttt ttcatttatt acaccagtaa atcttaacca ccgataagga
     8701 gcaaattatg cgactagcca gtcgttttgg ttatgcgaac cagatacgcc gtgaccgtcc
     8761 gctgacacac gaagaactga tgcactatgt gccggggatt ttcggggaag ataaacacac
     8821 gtcccgaagc cagaactata cgtacatccc caccatcacc gtactggaaa gcctgcagcg
     8881 ggaaggcttt cagccattct tcgcctgcca gacccgtgtg cgcgacccgg gccgccgggg
     8941 atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag aacatgtccc
     9001 tgaaattatt ctgctcaact ctcatgacgg tacctccagc taccagatgc tgccggggta
     9061 cttcaggttc gtctgccaga acggctgcgt ctgtggtcag tctctggggg aagtacgtgt
     9121 tccgcaccgg ggaaatgtgg tggacagagt cattgaaggg gcttacgagg tggtgggcgt
     9181 gtttgaccgg atagaggaga agcgtgatgc catgcagtcg ctggtcctgc cgccaccggc
     9241 acgccaggcg ctggcacagg cggcgctgac ttaccgttat ggtgacgaac atcagcccgt
     9301 caccaccgcc gacattctga caccacgacg ccgggaggat tacggtaagg acctgtggag
     9361 tgcttatcag accatccagg agaatatgct gaaaggcggg atttccgggc gcagtgccag
     9421 aggaaaacgt atccataccc gggccattca cagcattgat accgacatta agctcaaccg
     9481 cgcattgtgg gtgatggcag aaacgatgct ggagagcctg cgctgatgcc gtttccctga
     9541 gtgagggaat cattcagctt tcgttcctga aaaacacaca tttatttgtt atttaaggag
     9601 ttatctcatg aagaccgttt ctcagaatac ccccacaatt tattcagcta caacaccaga
     9661 gaataatccg cctcagctgg ttgccagcct cgtccctgat gaacagcgca tcagcttctg
     9721 gccgcagcat tttggcctca ttccacagtg ggtgaccctg gagccccgta tcttcggctg
     9781 gatggaccgt ctgtgtgaag actactgcgg gggtatctgg aatctgtaca ccctgaacaa
     9841 cggtggcgca tttatggcac ccgaaccgga tgacgatgat gacgaaacat gggtactgtt
     9901 cagtgccatg aacggtaacc gcgctgaaat gagcccggaa gccgccggca ttgctgcctg
     9961 tctgatgacg tacagtcatc acgcctgtcg tacggagtgt tatgccatga cggtccatta
    10021 ttaccggctg cgggattacg ccctgcagca tccggaatgc agcgccatta tgcgcatcat
    10081 tgactgagga gagaggccat gcaacagctt tcctttctgc ccggagcgat gacgcccggc
    10141 gagcgcagcc ttattcagcg ggccctgaaa accctggacc gtcatcttca tgaacccggc
    10201 gtggccttca cctccacccg tgcggcacgg gaatggctga ttctgaacat ggcgggactg
    10261 gagcgtgaag aattccgggt actgtatctg aataaccaga atcagctgat tgccggtgaa
    10321 accctcttca ccggcaccat caaccgcacg gaagtccatc cccgggaagt gattaaacgc
    10381 gccctgtacc acaatgccgc tgccgtggtg ctggcgcaca atcacccgtc cggtgaagtc
    10441 acacccagta aggcagaccg gcttatcacc gaacgtctgg tacaggcact gggcctggtg
    10501 gatatccggg tgccggacca tctgatagtc ggtggcaacc aggttttctc ctttgccgaa
    10561 catggtctgc tttaacccgt cacaaccaca tcacacctgt tttcactttt atcttctgtc
    10621 ttcagaggta tcccattatg aaaattatca ctcgtggtga agccatgcgt attcaccgtc
    10681 agcatccggc atcccgtctt tttccgttct gtaccggcaa ataccgctgg cacggcagtg
    10741 ctgaagcgta taccggtcgt gaagtgcagg atattcccgg tgtgctggcc gtgtttgcag
    10801 aacgccgtaa ggacagtttt ggtccgtatg tccggctgat gagcgtcacc ctgaactgat
    10861 gttccgtcac ccggaaaacg agtacgactg gctgagctca gacgccgctg tggatgaggt
    10921 cctgctcatc aacgaataca cctttaccga agccggtctg cgcgccggct gacccgtcat
    10981 cgttaaacta tcattcaccg attttctgtg aggattttat cgtgtcagac acactttccg
    11041 ggacaacgca tcccgatgac aatgacgacc atccctggtg gggactaccc tgcacagtga
    11101 cgccctgttt cggcgcccgt ctggtgcagg agggtaaccg cctgcattac cttgccgacc
    11161 gtgccggtat cagaggccgg ttcagagatg cggatgcgta ccctctggac caggcctttc
    11221 cgctgctgat gaaacaactg aaattaatgc tcaccagtgg tgaactgaat ccccgccatc
    11281 agcataccgt cacgctgtat gcaaaaggtc tgacctgcaa agctgacact ctcggtagcg
    11341 gtggttacgt ttatctggct gtttatccga cgcccgaaac gaaaaagtaa accaccggaa
    11401 taaccttctg ctccggcctg gtgctttcac cacgccactt ttccattttt catctctgca
    11461 tatcaggaaa accttcagta tgaaaacatt acccgataca cacgtacggg aggcatcgag
    11521 ctgcccgtct cccgtcacca tctggcagac tctgctctcc cgactactgg gccagcatta
    11581 cggccttaca ctgaatgaca caccgttcgc cgatgaacgt gtgattgagc agcatattga
    11641 agctggcatt tcactgtgtg atgcggtgaa ttttctcgtg gaaaaatacg cgctggtgcg
    11701 taccgaccag ccgggattca gcacttgtcc ccgctctcag ttaataaaca gtattgatat
    11761 cctccgggct cgccgggcga ccggcctgat gacccgcgac aattacagaa cggtaaataa
    11821 cattaccctg ggtaagcatc cggagaaacg atgaaacttt ccctgatgct ggaagccgac
    11881 agaattaatg tgcaggcact gaacatgggg caaattgtcg ttgacgtcga tggtgttaat
    11941 ctcactgaac tgattaacaa ggtcgctgaa aacggttatt cactccgcgt ggtggaggaa
    12001 tccgaccaac agtcaacctg cacactacca ccgtttgcaa cccttgccgg catacgctgc
    12061 agtaccgcac atatcacgga aaaggataac gcctggctgt actcgctgtc gcatcagacc
    12121 agtgacgttg gtgaatcaga atggattcat ttcacaggta gcggatatct gttacgtacc
    12181 gatgcgtggt catatccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    12241 cgtctggtta tcacacttac ccgacgttat ggcgtcagtc tcattcatct ggatgccagc
    12301 gctgaatgcc tgccgggttt acccactttc aactggtaac caggaacaac atgaaatcat
    12361 taaccacgga aaccgcactg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    12421 aatcgggaat tatctttgac aacaatgagg ataaaacgga ttcagcagca ctgttgccct
    12481 gtatcgaaca ggccagagag gatatccgta ccctgcgcca actgcagctt cagcaccaga
    12541 accggtgagt ctcactcatc atctcactca ccagacttca ttccactgac gccagcctga
    12601 acacggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtct gaaatcacag
    12661 tctcccgtcc ggaagtggtc aacgagaata cggacgttat ctgctccacc tcagtcaggt
    12721 acaggtcact ggaatatgat aattttccgg aaatcagcga agcgaacatt ctgagcacat
    12781 ttgaacaact gcaccagaac aaagatgaag tgtttgaacg gggagtgatc aacgtcttca
    12841 aagggctgag ctgggattac aaaaccaact caccctgtaa attttgcagt aaaattatcg
    12901 tcaacaatct ggtgagatgg gaccagtggg gataatgata tggtcacccc caccttaaca
    12961 gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga acatcacact
    13021 tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca agtctggtaa
    13081 ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg cgacatgtcc
    13141 gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc gctgtatctc
    13201 tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt ttgtcaaaag
    13261 taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac gaccatcaat
    13321 gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac accgggtcag
    13381 agaatcgctc atcagagata aagtgaaaac aaccaaccag atccacggtt tcttgctgga
    13441 atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc tcgtgttggc
    13501 tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata cccactactt
    13561 ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa tcaacgctga
    13621 tgataccgct cagcgcatta tgacaattcc tggtgttggt ccgattactg ccagcttact
    13681 atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc agggattttg cagcttccac
    13741 aggacttgtt ccccggcagt atagcactgg aggaaaatcg actctgttgg gtatcagtaa
    13801 gcgaggtgat aaaaatttac gccggttact ggttcagtgt gcccgctcat tcataatgcg
    13861 acttgaacgc aagcatggaa aacttgctga gtgggttcgg gagcaactca acaagaagca
    13921 ttcgaatgtg gttacctgcg ctctggcaaa taaactggct cgaattgcct gggcaatcac
    13981 tactcagaaa aaagaatatc aggcctgata tagatgctga tataactcaa ttagtttatt
    14041 ttaaacaata agatacaagt tacctttctg gttttgcgaa gactgattat tgatgacatg
    14101 aacggcaaat cgacctgatt aaaaacctaa cagatgaaat ggctcactga agccgagggg
    14161 atttttagga ttatcaggtg cggaactcat cggggcgcgg gcatccttgc ccacagagac
    14221 gccggataga tttaagcaag ccaaccatta atcaaaaatc agtgttgcaa aaacgggggt
    14281 gaccatagat ttcatcttat cagtggaatg caggcagatc gcctggctga cctggaaaga
    14341 atgttgcatc tgctcagcgg taaaccgatc cccgacaacc gagggaatat caccattaat
    14401 ctggatgacc acatacagtc cgttcagggt aaaggacgct atgaagatga gatgttcatc
    14461 attaaatact ttaagaaggg tggatctgca cacatcactt tcaaaaggct ggagctgatt
    14521 gacagaatta acgatataat agccaggcac tttccttctg tgctctcagc ctgaccccga
    14581 gtttgattcc ctttcgatat caaaagggac tgcgggtaca aaagagggta catatttcac
    14641 caaaccaaac aaaataaact aatatcaaca tgatagaagc attcttcgat tccgagtccg
    14701 gcaccactaa ttcttaagaa cccgcccaca aggcgggttt ttgcttttgg atctgacaat
    14761 aaccttcacg aaaaaagttt gcttataaag tctgggggaa ttactctcgc cacgttaacg
    14821 agagtaattt tattgatact aatctcctga tactttaccc ccgtccaaac tccagccgct
    14881 gcacattcac catcccagtc ttctcagcag cactggcatc aatttgtgtc acccgcagcg
    14941 catacttttc atccagcgcg tttaaccagt tcagcaggtc attaaacacc accggatcta
    15001 tccagacctg aatattctcc ccacgctcgg ctatccgctt gatgactacc gagtgcgcgg
    15061 aagcgctgtc actgataacc cgcgatacct gcgcaggcgt tgttgtggcg gattttcgcg
    15121 ccaccataat atccggcgcg gcgctcctca gtctggtgtt catcgccacc agttgctgca
    15181 acattgtctc ctgttgttct atccgttcgc tcaacggctg ccagatgagg acgtaatatc
    15241 cgacgctaaa caggaacacc accgctgcca gtaacatgcc cttttcacgc ggagaacgcc
    15301 ccgccaggtg ttgtgccagc cagtgttcgc cacggcttaa ctggcgttca cgccattgct
    15361 gaaaatagtg aataaattta tcgcgtaaca tgttatttcc tccgcaacgt tacgccgccg
    15421 gaaaccgcat caccctcttt ctgtaatgcg tcctgttgca caacataatc ggttgccagt
    15481 gcactacgca gtttgtcgaa actggcaaag ttagcagccc gcaactggag gtgaagcgtc
    15541 tggcgttttt gatcaaaggt gaaaccacgc atttcgatgt cggaaagtga cgctgatttc
    15601 agggtgctgg ctatcgctga caactcggcg agcagccggg tatcgtcggc ctgtgggcga
    15661 tattttttca gcaccatcgt cacctgagag cgtaaattca caatccgctt ctgctccggg
    15721 aacaacgtta agaactgttc ctccgcctgg gtgcggcttt gcgccacctg ttcgctgacg
    15781 ctccataacg tcacgccccg ctccactgcc agcgcaacca gaatcagcaa tatcggcaga
    15841 atcatcaccc gccagcgcgc ccactgtttt cggtagctga cgcgaggctg ccacggccct
    15901 gtcagcaggt tcccttccgg ttcgccataa gtggtaatgg cgggcagagc tgtaacggtc
    15961 aggcgttcag cgtctgcgcc agcccatgct gatagctttt ccggtgcaat gccgactacg
    16021 gttagcgaaa gcggtaaatt ctgctcattg agctgggcgc ggaacatgac cggagccagc
    16081 gcccgcccgg cgctccatcc ccggcattca tcgatgcggc agataacccg ttgcgcatcg
    16141 ccagccataa acccacaagg aatggacatc cagtccggcg cgacggtagc gcgggtgatg
    16201 ccgttttcct gcaaccactg cgcaatgttg cgcatatgct gttggtgaat cactgctacg
    16261 gttgccagtt gctggtcgat tttcaacggg gcgaaatgca gttcatcgat atcctggttc
    16321 agctcttctt ccagcaaggc gggcagaata gtcggtatct gcttgcgggc gacatcaggc
    16381 agttcaacct gccagacgct gatccattcg ccgggaatgt agagtcgaat cgcatcggtt
    16441 tgcagccatt gctgaagctg ttcgtcagca acgtcaggcc agataccgtg ttccacgtcg
    16501 ggtgtacggc gctgccagcg gacggtatcg gaggcgcaaa gcgggaaaaa aatctcaagg
    16561 atggaactca ctcactttct cctgtctgat gccagagaac cgaaaagtgt tgtgggccca
    16621 tgcggacaat taacgagttc atcgtcagtt caatctcatt cacggtgata tctgaacgca
    16681 gccagaagta attgctgtcc acgctcagga cggtttttag ctgtttttta gtacgctcat
    16741 cgacgtcagc aagtagcggc tgtgcaagaa actgatcgac atcttcccag cccttcgccg
    16801 gacgttgttg taataacgcc cgcgcctgaa cagggcttaa ccacgggtca aacagcgcct
    16861 caagaatcac actttgcgtg acgtctaagg tattgatgtt gatttgctgg cgggtcatcg
    16921 gcagcgcaca gaccagcggt ttcagttttt gataaagccc ggcgtccatt ccctgtacca
    16981 cgcgcatctc actgatatca gccagcggtt gattcgcggc gtagaaaggc accgaacggg
    17041 cgagatactc gctgtcttca cgccccagcc gcgtctgcac gctgcgatct tcgtcaataa
    17101 actcccacag gctttcggct atcagttcgg ctcgataagc aggcacatcc aggcgcgaga
    17161 tcagggcaat caactgttgt accgcaagcg gacgcgacgc cgttgtcggc tgagcaaggg
    17221 cattcaggtt aaagcaagcc tgtgcgtcac gcagggtgac cgcaatttgc ccctgcggca
    17281 gagggaagaa acgagggccg gatgcccacg gctgtgccag atgcacgcgc ttttcatttt
    17341 tcagtctcag actgagcgcg ctcagcgcca ggctttccgc actggcgctg taccacagcg
    17401 cctgctggta ctcctgctgg ctgcgcgttc gcccaagttg ttgctgcatc cgcccggaaa
    17461 gcgtgatggt taccagcatc ataaccgcca gcaataccag caccacaacc agtgccattc
    17521 cgcgttttgg tggtgaggtg atcatgataa ttgcggcccg cgtaacaacc agatgcgttc
    17581 aatctcgccc cattgcggcg aatgcagagt catgcgcact gccacaggga tcgcctgtac
    17641 tgacgaccag ctctcctgcc agcgcgtacc atcatagaac tgcaaacgga gcgaatccgc
    17701 cggaatcaac ttttgcattg tcggcttcac gctgcctgcg gcatcggtca gcggccaggc
    17761 taaccgttcg agataaccgt catgaatgcg gtaaccgacg gtgagcagat tgctgcgtgg
    17821 cagacgcatc aacgggttaa ccacgccgcc gcgcacaaag cgcatgcctt cactctcaga
    17881 cgccagcacg ccagcgcccg ccagtaacgc tggttcacgc tggccctgtt cgcctcttac
    17941 cggacgcggc atcatttgtg tcagatcgtg ggtcagaaaa ctcatcgttt gctgcatgag
    18001 gtttagtttt tgatcatgtc cggcgacggc gttattcacg cgtgtgacac agtttgtcac
    18061 ctgctgcgcc atcagtgcca gtgaggcaaa aatggcgatg gccaccagca tttccagtaa
    18121 cgtaaaacca gcacgagtcc ttctcactgt tggcctccca cggcgctaaa ccaggcgcgt
    18181 cgtgactgaa tcaccgacga aaagtcttcg tgaaggctga cttcaatatc caccgcctgg
    18241 agcagcgcgt tgcctgtact cagcggtgtg gttcgccagt accagcggct tcctgccata
    18301 tcgctctcgc cctgctgcct gaccgcccgc tgctcgcggg tcatcaggta gagcagcgcg
    18361 gtctggtttt cagccaccca gcccgctacc gttttttcct caagaatttg ctgattactc
    18421 agcgcaccgc tggcaatctg taacaccgcc gtggcagcca gcgcaaaaat cgccagcgcg
    18481 agcatcactt ccagcaaggt aaatccgcgc ttcatggcat cctctcatca cattgattga
    18541 gggataacgc gccatcgtgt gccagcttta ccgcccaaca tgcgttttgc gccgccgagt
    18601 agaagcgcag cgtaaacggc gtggcgggtt caaaaggcga aaaacggatc tgcggcgtct
    18661 tctttttcgc ctctttttgc aactccagtt caatatcgtg cagcgtcagg cgacgacgtt
    18721 gcagctccag cgcatattcc ttctgccaga tatcacttcc gggttgtaac agcatctgca
    18781 cctgtttcgg caccgtaacc tgtgccgata accgggtcgc agaaacgggc aaccactgcc
    18841 cctgacgacg ctgcataaac tgatagccag gcgggtcgat tagcacaccg agtgttttcc
    18901 cttcgatcac tgctctgtcc ttaaactgcg caaaacgagt cagaaaatcc tgcgccgctt
    18961 ttttcgcggg cgattcagaa tcggtcgcaa aagtctgtac cacgcccgca ctggcaaggc
    19021 cgataaggaa aatcaccagc atgatttcca gaagtgtaaa tccgcgttca ggcactgctt
    19081 actgaaactc ctgcaaattc caattaccga tatctgcgcc agcgccttcc ccgttttcct
    19141 gaccatccgc ccccagggta taaacatcaa acagcccttt ttcacccggg ctgagatact
    19201 gataatcatt gccccacgga tcctttggca gtcgtttaat gtatccacca gtacggtagt
    19261 tgcgggcgtc cgccatattg gctggttgct ggatcaacgc ctccagtccc tgttcggtgg
    19321 tcggataacg cccgttatcc agtcggtaca tatccagtgc gttttccagc gccacgatat
    19381 cactgatggc tttttgccga tcggctttct ctttgttgcc caacaggtta ggcaccacca
    19441 gacttgccag gacgccaaga ataacaatca caaccatcac ttccagcagg gtaaaacctg
    19501 cccgtggttt ttgtgtgcgg gataacgaat tcatgtgata actccgtaaa ttacattcca
    19561 accatattgt tcagttgcag catcggctcg aggatggcga tgacgataaa cagcaccacg
    19621 cccgccatca tcaccaccag cgcaggctca aacagcccca acgccagccc cacctgggta
    19681 tcaaattccc gttcctggtt gactgcggcc tgctcaagca tggtttccag ttcgccgctc
    19741 tgttcgccgg aagcgatcat gtacagcatc attggcggga acagccgcaa ctccgccagc
    19801 gcggcgcgca gactgcttcc ttcgcggacg cgatctgccg ctagcagcag ttgttgctcg
    19861 acatagcgat ttgccgacac agcggcagcg gtctgaatgc cttccagtag aggaacactg
    19921 ctggcgatga ggatgcttaa ggtgcgggaa aaacgcgccg tattcagccc gcgcgcaaca
    19981 cgccccgtca caggcaggcg cagcacggtt ttgtcccagc gcaggcgcat agcaggattt
    20041 ttaagtagcc gttgccccag caccagaagc cccagcaaac ccgccagcca gtaaacgccg
    20101 ctagcctgta acgcatcgct catggcaata agcgtgcggg tggaggcggg tagcgcgtgt
    20161 ccgagatgat caaactgttc gatgattttc ggcaccactg ccgtcagcaa aatagtgact
    20221 acgcccgttg ccaccaccag cagaaccagc ggatagagca tggcctgcaa tagacgtgat
    20281 ttcaggcgct gtcgctgttc ggtgtaatct gccaggcgat tgagcaccac gtcgagatgc
    20341 ccggattttt ctccggcagc gaccatcgaa caaaacaggg aatcaaagac gcggggatgt
    20401 tcacgcaggc tgtccgacag ggtgtaacct tcctgaatcc ggctgcgcag cgccattccg
    20461 aggcttttta catgcagttt ttcactttgc tcactgaccg cctgtaagca ggtttccagc
    20521 ggcattgctg cctgtaccag cgttgccagt tggcgcgtga acagcgccag atctgccgcc
    20581 gccacgcgac gatgtgcgtg ccgccgacgc tgcaacatcc cccctgacga agtattcagc
    20641 cgggcttcaa tgtgcacggg aataagctct ttaccgcgca acaactggcg ggcatgacgc
    20701 gcggaatccg cctcaatcat acctttggtt ttgcgaccat tacgctccag cgcctgatag
    20761 taaaacagtg ccattacgcc tccatggtta cccgcagaac ttcatcgaga gaggtttctc
    20821 cggcgagcac tttctcaatg ccgttgctgc ggatacccgc agagtgttgt cggacataac
    20881 gttccagctc cagctccccg gcctgacggt ggatcaaatc acgcaatgtg gcatccacca
    20941 cgatcagctc atggatggca gtacgtccgc gaaaaccttt gtgattacag gcggggcagc
    21001 cctgcggatg gtacagagtg acggtacggg cgtcggtaat tcccagcagg cgtttttctt
    21061 cgtcagtggc aggcgcagcc tggcggcagt cgggacacag cgtgcggacc agtctctgcg
    21121 ccatcacgcc cgtcagactg gaagagagca ggaaaggctc cacgcccata tcctgcaaac
    21181 gcgtgatcgc ccccaccgcc gtgttggtat gcagcgtgga aaggaccagg tgtccggtaa
    21241 gtgaagcctg gacggcgatt tctgcggttt cggtatcgcg gatttcaccg accatcacca
    21301 catccgggtc ctgacgcaaa atcgcgcgca ggccacgggc gaaggtcatg ccgacgcggg
    21361 tgttaacctg cgtctgaccg atcccttcaa tcatgtattc gatggggtct tcaaccgtga
    21421 gaatgttgcg cgaatggttg ttcagctcct gcaatccggc gtacagcgtg gtgcttttgc
    21481 cggaacccgt cggtcccgtc accagaaaga tgccgtgcgg tttgtgtaac agctggcgca
    21541 actgcactgt cagttgctgg ctaagcccca gacgctccag cgtcaggcgg gcctggtttt
    21601 tgtccagcag tcgcagcacc acgcgctcgc cccaggcgga aggcatggtg gagacacgca
    21661 catcaatcgc ccgaccgccc agcagcagcg caatacgtcc atcctgcggt acgcgctttt
    21721 cggcgatatc cagccgcgcc atcaccttga tacgcgatac cagcagcgag gccagtttgc
    21781 gccccgggcg cagcatttca tgtaatgtgc cgtcaacgcg aaaacggatc accagactct
    21841 tttcaaacgt ctcgatgtgg atatccgaag cgccttcttt aatcgcctct gccagcatgg
    21901 cgttgatcag tttgatgatc ggcgcatcgt cgtcactttc cagcagatct tccgtttccg
    21961 gcagttcttc tgcgagggta aaaaagtcct cggcggaacc gagatcttcc atcaactggc
    22021 gagcttcgga agagtcccgc tgccagaccg cattcagccg ctgttcaaat tcggcctcgt
    22081 cgatttgccg cagcgtaaag ggcgcgttca gcccccgctg cagctcctgc aaaacagaga
    22141 gcgatagcgg atgaacgtgg aggatctcca gcgacgcttc gcaccatgcc accaggctaa
    22201 accgacggct gaaactgtag ggcagacgca cggtgttggc ggtggtttcc tgtgctacag
    22261 gcaccattaa cgcgttctcc cggcattgag gaacgcgcga acttccggcg gtaaggcctg
    22321 gttttgcgct ggcagtaccg gctgcgcggt gtgcggcatc aggcttaagc cttgctcatc
    22381 gcgatagatt tgttcggcgc gcatatagtt atatttgcgc tgcgacacgc catctgccgc
    22441 cataccgtca cgcagaatgg tcgggcggat aaataccatc aggttacgtt tttctttttt
    22501 atccgccgtc gatttaaaca ggttaccaat caacgggata tcgcccagca gcggcacttt
    22561 cgccacgctt tctcccgcct ggtcgtccat cagaccgcca agcacaatca gctcaccatc
    22621 gttagccagc acggtggttt tcagtttgcg ctcaccaaac accacgtcga ggctggtctg
    22681 tccttccacc ttcgagactt cctgctcaat caccatctgt accgcgttac cttcgttaat
    22741 ctgcggcgtg actttcagca tgatgccgac ttttttcctt tctactgtat tgaaaggatt
    22801 gctgttattg gagccaacgg tagagccggt taataccgga acgtcctggc ccaccatgaa
    22861 gaaggcttcc tggttgtcca gcgtggtgat gctcggcgtg gagagcacgt tagagctgga
    22921 gtcgttttta accgcctgca ccagcgccat ccagtcgcct ttcaccacac caaccgccgt
    22981 accgctaaag ccggaaagaa gctgggcgag tgtggagaga tcgccgttgg tatccggatt
    23041 aatggtggta gcgccgtttt cactgatcac cgtcgagcct ttctgcggtt tcgcctgaga
    23101 aatggctgcc cccagcgtac cgatagggat ctgcgtacca ttagcaaact gcattaatcc
    23161 ggcatctttc gacgcccact gcacgccgaa gttgatattg ctaccttcgg caacttccac
    23221 gatcaacgcc tcgacgtgca cctgagcacg gcgaatatcc agttgttcaa tcacgctttg
    23281 cagcgactgc atgatgtcct gcggtgcggt tacaatcagg gcattactgt gtttgctggc
    23341 ggcgatggat acaatctcac gcccgctacc gaccgtgcct tccgcctctt ctttagccgc
    23401 cgtgagcgtg ccgctgacct gcttcagcac atcgaccaga tcttcggctt tgctgtattt
    23461 gagatagaaa acctggctgt tgccgctgcg ttccatttct gagtccagcc gacggatcag
    23521 gcggcgcatt ttgtcccgcg tggccgggtc accactgaca atcacactgt tggtgcgttc
    23581 gtcggcgaca atttgagatt tcagcgtcgc tggctggttc tcgccgctgt ttttcgtcag
    23641 gctttccagc acgcgggcga tttccgaagc cgaggcgtta tccagcggga tcacctcttc
    23701 ggtgcgatta cccgcgtgat ccacgcgctg gatcacttcc gtaaggcgct ccacaacgga
    23761 ggcgcgtccg gtgagcataa tcacgttgga gggatcgtaa ttaacaacgt tgcctgagcc
    23821 tgcgctgtcg atcatctggc gcagaatcgg tgccaactcg cgtaccgaca cattacgtac
    23881 cggcactact ttggtgacca tttcatcgcc cgcgtagttg tcgctgcctt caccgaccag
    23941 cggcagcggc tcgactttcg cagcgctgga tttcaccacc ttcagcacgt cgttttccat
    24001 tggtacgacg gcatacccct gtgcttccag caggttaagg aatagctggt aatactggcg
    24061 ttcattgagc ggggtcatgg tgcgaatact cactttcccc tgtacgccct gccccataat
    24121 gatggtttta ttaaggttag cgccgacggt ttcgatgaac gatttcaggt cggtatcttt
    24181 aaaattagcg gtgaaagtgg cttcttccgc ccagaccggt gaactgcata atgccgctgc
    24241 cagcaccagc ggcagtaaac gcttttttgt tttgaggcca gttgtcttct tacgccagac
    24301 cgacaacgta atatcacgcc aaaacacgat gaatgattct cctggattaa atgcggttag
    24361 cgcagcgcga tggaaatgtc gtggcgcgca cccttgcgta aaaccgtaag ttgaatggaa
    24421 tccattgaag gtaactgccg catcagagca atcattgctc gtggatcagt gaaatcctgc
    24481 tgatttagcg caatggcgat atcgccttcc ttgaaaccgc tggcatcgaa cagagaacga
    24541 tctgcccccg gcttcactgc ataaccgaca attccctcct tacgcacagg cgtaagctgg
    24601 atatagttaa aaattttctg cggatctttc gccagtgcct gacgcacggc agtcggtatc
    24661 tcaactggcg cactgacagc aggttcagca acagcttgct ttgcttcgtc actgacagct
    24721 tttttgttgg tcgcggcaac ggtggaacgc tcctcttcag ccaggctcag gcgctctatt
    24781 tttccctgat agcgcagcat cacatggtcg cgattgattt cctcaatcac cgcgttgtga
    24841 gaaccaagcc gttccccctg caaatagacc tgctgtttgc caccttcttc aataaccgcg
    24901 ccgggtctgg caccaaaggc gatcccacgc aacaccacat taagacgcgt ttctgccaca
    24961 ggtgcaggtt cgggttgttt taccggcgtg gcgacgggct gatatttgcc aaaccagttt
    25021 tgctggctga ttaattgcac gtcattttta tcgaacgttt ttgcatccgc acggggcggt
    25081 ttattcgccg atggggaaac agccgtatat tccgcagaaa aagagaaata gcgccagagt
    25141 gaatgcgcca cttttgcaga aataataagc agcatcagcc agaacatccc gcgtacaatt
    25201 tttcgcagat gctctttgtc tgtattaaga gattgtctct ggttaagagt gtgacgaatt
    25261 tttgtcagcc attgaatgag ataaattcgt gcgtcacgaa aaacaacccg cgccaattta
    25321 tatctacccg acgttatgct ttgactattc cacaggtggt acgatccagt ttccgctgat
    25381 actggttacc tgtacgtgta tcattaatcg ttatgctgta attaattccc tcagtgagca
    25441 ttaatttcac tgttgggtca gtgcacattt gtcgttgata gctggttaaa aaggcgtcag
    25501 gcgtctgcgt ggtctgagtc cccgcttcgc tgataatggt catttttacc gtcgtgccgc
    25561 tactttgtgc cagcactaag gtatatcccg cagatttaat cggcaggttt tggctgatat
    25621 tttgcgcctg ttttttcgcc agtaaactgg cattttcatt atggctggca cagccactta
    25681 ataatcctgt aacgctcaac aatagcgata ttaataccct ccctggcatt tgttttatcg
    25741 acatagtaaa taatctctaa ggttattaat aagagttaaa atgtcacttt gataatgacg
    25801 tggttatcat taaaacaatg cctgtagata aagtgttgct ataccgccca gacttaaaca
    25861 cggtccaaaa ggcagtgtgg ttgatcctct ttttgtaata atggcatata tcaggccgca
    25921 gcatgatgcg attaaagcaa cattgggcag tgataacggc cccacccagc cacctaacgc
    25981 agcgaacaat aatacatcgc ccatgcctaa tgcttcttta cgcagaacta ttccggctat
    26041 ccagcgcagg gagtaaaaag tgataaaccc caccaggacg ccggtaactg cgtcttgtag
    26101 cgttaacgga ctctgttgcg cccatgccgc aatcagtcct gtccacaata cgccctgagt
    26161 aaaaacatcg ggcagccatt ggtgatcgag gtcaatgacg ctcgcggcaa tcagccaggc
    26221 ggataatatc atcaccgcca gcgcccatcc actttccggc cagaccagac tcgccagcaa
    26281 aaaagcgagt gctgtcaata actccaccag cggataacgc ttgctgattt tcgcctgaca
    26341 gtcgcggcag cgccctttga gcatcagcca ggagagcagc ggaatattgt cacgtatccg
    26401 gatggtctgc tgacaatgtg ggcagtgcga acgcggtagc gcaaggctta tttttgactg
    26461 cgcactcggc atttctccat gaaactccgc catttgttgg cgcagcatga tggggtaacg
    26521 ccaaatcacc acattcaaaa aactaccgat gattaatcct ccgacggtag ccagtatggg
    26581 catcgccacg gggtattgct gaaaaacatc aaaaagcatg gttaaaggtt gtttgttgta
    26641 acttgccgga tgcggcgtaa aacgccttat ccggcctaca ggtatctgcc aacgcaagta
    26701 atcgcggctt tccctgaaga cccgataaga tggcgcatcg ggcatatgtg ttaacccgat
    26761 gcgccttatt tcatgctgga tgcggcgcga gcgccttatc cggcctacag gtgtctgcca
    26821 acgcaaataa acgcagcttt ccctctctgt aggcccgata agcttgcgca tcgggcatat
    26881 gtgttgaccc ggtgcgcctt atttcatgcc ggatgcggcg cgagcgcctt atccggcata
    26941 caggcttact cggctgacat cttatgctcg gtaacctgat taatggtttc cggcccttgc
    27001 tgcggtttcg gcagaccaag cgacgccagc gtgttgtatg ccgactggct cacaccgccc
    27061 tcgaagctca tctccgtcgc tcccggcaac tggtaagcat tcgcgcccgg attccatttc
    27121 ttaaagaatg cggaaaggtc cgtctgagcg acccaggagg cacacagcat cagcgtgtcc
    27181 gcagcgttac cgttggattc agcacagtaa ttcttgccac caaaggtctt gtcaccaacg
    27241 tcatcgccgc gtgctttacg atgcatcaac tggaacaggt tccagccttt catcccttca
    27301 cgctcgctgt agaacttagg caggttacct tctggatacc actgtttgat atcaaagttt
    27361 ttctctgccc attccttcag ctgcgcgtac atcagcagac ggtcacccgc accgccacgt
    27421 gcccatgcct ggttgttgct ctcctccaga tattccggtg cgacggtaat atcgtcagca
    27481 acacggttca tcttgccgag atagcgatcc tgcatgtaca gcgccagcac gttgttcgcc
    27541 acttcggtcg cgcctggaac agtcagcggc gtttctgcgg cgttgtgccc tacttcgtgc
    27601 cagatcaacc agtcgttcag cggcgtcgtc ggcagcgtgg tgctgttcgt cgagaagctg
    27661 ctgttcatta ccggataacc cgagtgcgca tcaccgatgg agatctgcac atcgttggtg
    27721 aaacgatgtt tgtgacccgt caatgcttca taggtaaaca tccggtggtt accgctttcg
    27781 ccattacgac catagaagtc attcatcgag ctggcaaagg tgtccagatc gttagcgaat
    27841 tgcttcagtc cgccagtgta attgctggca ttcaggttct tcttcggtgt ggtatagacg
    27901 aaagcgtctg attccagctc acccagcgga gccggtgagt tcagatcgtt tttccatgcg
    27961 ccgtctttat agaacggtgc ttttaccacg ccagtaaagg tgaagctggc agattcattg
    28021 gtagagctat tgcccttgat ataaatcagg ccaccgtaag gcaccttgaa cttcaccgta
    28081 ccgctagcgt ccagagagta cgttttagtc actcttggcg gacggttcag cgcaacttca
    28141 tgcttctcac gtccggtcag gtcgtcagcc agcgccacgg tgacggtcac aggaacgttc
    28201 gcattggact taatggtgac ctctttctga gccggtgccc acaggccagt tgactgcatg
    28261 ttacctgcaa accatttggt cggattcgag tacaggctga tggtttcagt aacgttctgt
    28321 ccctcttccg atactgctcc cggatacttc tcgacatcaa ctttgatgtt cagatcccac
    28381 caggaacggc ccagcatcag gcgtgtcagc ggtttttcca tatagttgag cgggtagctc
    28441 gggttcatca tgcccgcttt gctgctaccg tcaccgtaga tcatgttgtt atcgaccagc
    28501 gattttttca gatctgcaga acacttggtg ccgcctgcat aggcatcatt ggcgtagcag
    28561 ttcaggaact cggtgaacgt tttaaagccc agctcgtcat ttttgccttc ttcataacga
    28621 tagctcgtat cgttccacag ccagaccgac atgttctggt acagacgttc cagatcgacg
    28681 ctgctcagac gctcaccttt gcgaccattg gtccggaagt agagctcatg ctgatacaga
    28741 cgctgaatgt tggtgcctaa atccgcagcc tgcaccatcg cttttgcagt gtcggcgtta
    28801 aggcttagtt gcgtatactg tggaacatac atgccaccag taaccggaac ccccgtgcca
    28861 ggacgatatt ccaggcagtt gacctcatag tgatatgccg gattagtaca ctctttcagc
    28921 cccgggaacg cggcgaaaat tttctccttc gcagccttca gagaatcctc tgttttatga
    28981 tcggcctcat caataaaggc ataacgcgtt tcctgtttgc catctacatc ttccagccag
    29041 ctggcaactt ccagcttcgg tttgtcatca ggtttgtttt ctacctgata tttccactta
    29101 acttcccctg tcttactatc gatggtgtac ggcagcgcac catctacggc aggataacgt
    29161 tcatagaccc aaatgcccgt tgcgcgctgc tgacgaacgc ggttcggata cccttgcgga
    29221 tcgttattta ctaccgactt gttcagtgcc atcgacagac ctgcggcatc caacagacgc
    29281 acaaaaccag acgcgctctc ttccttaaga ttgctcatca cgttttccat gatcagcacc
    29341 gatccacctt tgttcagata ggcgatcaga tcggtcacat cctgctgagt cagcttcggt
    29401 ttgctggtat ctgcacgcag cgggattgca taaggatcgt tacccacctg agtcacatat
    29461 tcaaagccgt taaggatcag cagcggcatt tcctgcggat cgagatcgcc atagctactt
    29521 aaatgctcaa cagagatgcc cgcaaaatcc ggatggaagt cgaacgcagc gctgtttcct
    29581 gtaacctgac catgacgttt aaaatagaca gtatctaggt tggtgcctac ggtcatgctg
    29641 gctttcgcgt ccggcgtcca tttatcgtcg gacagatagc gcagcacgtt ctccatgaag
    29701 ttcttcatgt catccgggtc gctgttgagc gtacactgcc catctttatt aacgcccccg
    29761 ttccagctgt aaccgttcgg gcaacgcaaa atgctgttgt agtgtgggtt tccgataacc
    29821 atcagtttgc cctcaccgac ttgccccagc gaaataaacg gcaggttgaa agtggcggta
    29881 tcgcgcgtaa cgttttccgg ctcaacaaga gaaggcgctt ccgtaatgta cgccagctcg
    29941 tttttatccc aggcgcgttt ttcgccaaac gccagccagt agtttttatc attacgcgcc
    30001 atcagaatcg ggaaggccgc gttggagata ttcaccaccg cctgaccgcg cgcattaccc
    30061 gtgctgccat agaagttagt agagtcatgg aatacatgga acttgctgac agatttgtag
    30121 ttcgtatcca cgccccacag cttgttgata acgccctgaa tctggccgtc attaacattg
    30181 cgcgtcgtca gcgagaacca gcgagcctcg ttacaaccgt cggttttcgc acaaatcgcg
    30241 gtatcgatct ctttggcctg acccgtctta aactgctcga taaattcgtt tggcagatta
    30301 acaacttgct caccttcatc cagcgtcgca ccgttggata acgagagatt gataatctcg
    30361 ttaatcacgt tgggatattc ggcaaagacc ttgcgtacat catccggaac aacacgggta
    30421 ttattttgcc cggtcgtcga atagcgatga ataagctgat cgatattcgc cccgcgaact
    30481 tcatcaccca gttcagtcag cgcaatggtc gacttattgc cgcgcactga acccagttca
    30541 aaggtatcga taccaaagga gatggtttcg ccccagctaa aagaaaattc accattttcc
    30601 cctgtcacgc cacggcctga attggtgtag tagttgacgc cagcaacacc atatccctgg
    30661 ctatccacca ggcgaccttc tgagagaatg atttccgaag gttgatactg ataaaactgt
    30721 tccgcgttag ccgacacgaa ggaggcgttt agatccggtt ttgttcccgg agtcgtgacg
    30781 gggactaccg gtgaagtatg agtggatggc gctttatcag tcgcggcatt gttttccacc
    30841 tcttcattga ccagcttttt gaactcttcc ggtgccagat cgatttgctt atacagcgag
    30901 tcgaaacgtt tactctcgat caccgaggag aactccaggc acacctgttc tgtattcgcc
    30961 gggcaactgt tcatggaggt gaccagcgag agcgcattgc ttttcttgtt gtcggaaccc
    31021 gccagttctt gcgcgtcctc aagactaaac gacacttttt caaccgcacg caggctacgc
    31081 gcagcttctg actgggtgtc gaaggtagca attgtcgtgt tgttccctgc cacacaggtg
    31141 actttgtcgc ctggtgtaaa ggtaaagcca tcgctggatt catcattaca ggtgatatca
    31201 ccagttaccc gcaggcttcc gcccagggtc agataacccg ttttcgtagg aacaggttct
    31261 ggctccggtt ctggtgttgg ctcaggatca ggtgtcggtt ccggcgtagg ttctgggtcc
    31321 ggcgttggct caggcgtcgg ctccgggttt ggtgttggat caggtttcac ttccggcaaa
    31381 gaccctgttc cagaatctac aggcggcgta tcggaggaag atccggagcc accgccatca
    31441 caaccggcta acagggttgc actcaaaata gccgctaaa
//
LOCUS       DAPPNJ010000033        31301 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000033 DAPPNJ010000000
VERSION     DAPPNJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.033"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="RPM73_001209"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="RPM73_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDX6519588.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="RPM73_001210"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="RPM73_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDX6519589.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="RPM73_001211"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="RPM73_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDX6519590.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="RPM73_001212"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="RPM73_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDX6519591.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="RPM73_001213"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="RPM73_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDX6519592.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="RPM73_001214"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="RPM73_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDX6519593.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="RPM73_001215"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="RPM73_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDX6519594.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="RPM73_001216"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="RPM73_001216"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDX6519595.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="RPM73_001217"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="RPM73_001217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDX6519596.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12868..13407
                     /locus_tag="RPM73_001218"
     CDS             12868..13407
                     /locus_tag="RPM73_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDX6519597.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13817..14950)
                     /gene="thiH"
                     /locus_tag="RPM73_001219"
     CDS             complement(13817..14950)
                     /gene="thiH"
                     /locus_tag="RPM73_001219"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDX6519598.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(14947..15717)
                     /gene="thiG"
                     /locus_tag="RPM73_001220"
     CDS             complement(14947..15717)
                     /gene="thiG"
                     /locus_tag="RPM73_001220"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDX6519599.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15719..15919)
                     /gene="thiS"
                     /locus_tag="RPM73_001221"
     CDS             complement(15719..15919)
                     /gene="thiS"
                     /locus_tag="RPM73_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDX6519600.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15903..16658)
                     /gene="thiF"
                     /locus_tag="RPM73_001222"
     CDS             complement(15903..16658)
                     /gene="thiF"
                     /locus_tag="RPM73_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDX6519601.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16651..17286)
                     /gene="thiE"
                     /locus_tag="RPM73_001223"
     CDS             complement(16651..17286)
                     /gene="thiE"
                     /locus_tag="RPM73_001223"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDX6519602.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17286..19181)
                     /gene="thiC"
                     /locus_tag="RPM73_001224"
     CDS             complement(17286..19181)
                     /gene="thiC"
                     /locus_tag="RPM73_001224"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDX6519603.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPITAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19262..19362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19414..19890)
                     /gene="rsd"
                     /locus_tag="RPM73_001225"
     CDS             complement(19414..19890)
                     /gene="rsd"
                     /locus_tag="RPM73_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDX6519604.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19985..20758
                     /gene="nudC"
                     /locus_tag="RPM73_001226"
     CDS             19985..20758
                     /gene="nudC"
                     /locus_tag="RPM73_001226"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDX6519605.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20798..21862
                     /gene="hemE"
                     /locus_tag="RPM73_001227"
     CDS             20798..21862
                     /gene="hemE"
                     /locus_tag="RPM73_001227"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDX6519606.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21872..22543
                     /gene="nfi"
                     /locus_tag="RPM73_001228"
     CDS             21872..22543
                     /gene="nfi"
                     /locus_tag="RPM73_001228"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDX6519607.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22586..23176
                     /locus_tag="RPM73_001229"
     CDS             22586..23176
                     /locus_tag="RPM73_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDX6519608.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23363..23635
                     /gene="hupA"
                     /locus_tag="RPM73_001230"
     CDS             23363..23635
                     /gene="hupA"
                     /locus_tag="RPM73_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDX6519609.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23648..24343
                     /locus_tag="RPM73_001231"
     CDS             23648..24343
                     /locus_tag="RPM73_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDX6519610.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24345..24770)
                     /gene="zraP"
                     /locus_tag="RPM73_001232"
     CDS             complement(24345..24770)
                     /gene="zraP"
                     /locus_tag="RPM73_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDX6519611.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25008..26384
                     /gene="zraS"
                     /locus_tag="RPM73_001233"
     CDS             25008..26384
                     /gene="zraS"
                     /locus_tag="RPM73_001233"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDX6519612.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHLGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26381..27706
                     /gene="zraR"
                     /locus_tag="RPM73_001234"
     CDS             26381..27706
                     /gene="zraR"
                     /locus_tag="RPM73_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDX6519613.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27703..28992)
                     /gene="purD"
                     /locus_tag="RPM73_001235"
     CDS             complement(27703..28992)
                     /gene="purD"
                     /locus_tag="RPM73_001235"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDX6519614.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29004..30593)
                     /gene="purH"
                     /locus_tag="RPM73_001236"
     CDS             complement(29004..30593)
                     /gene="purH"
                     /locus_tag="RPM73_001236"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDX6519615.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            31210..>31301
                     /locus_tag="RPM73_001237"
     rRNA            31210..>31301
                     /locus_tag="RPM73_001237"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7411 a         8087 c         8550 g         7253 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tgcgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcag cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagaccccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacacc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgccg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg tatcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5341 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgacagacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcgggttaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggggcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
     9961 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtga ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caagggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaatac tgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggt cagaccatta ctcgtcagac
    11581 agacgaattg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag cacgtcgtcc
    11881 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggggc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg agtttaggga
    12721 aagagcattt gtcagaatat ttaaggaatt tctgaatact cataatcaat gtagagatga
    12781 ctaatatcct gaaactgact gaactaattg agtcaaactc ggcaaggatt cgatactatt
    12841 cctgtgtaac tttcttaagg aacgagaatg aaacaggaag tggaaaagtg gcgacctttt
    12901 ggacatccgg atggtgatat tcgtgattta tcatttcttg atgctcatca ggctgtctac
    12961 gttcagcatc atgagggcaa agagccttta gagtatcgct tttgggttac ctactctctt
    13021 cactgcttca caaaagatta tgaacatcag acgaacgaag aaaaacaatc gttaatgtac
    13081 cacgcgccta aagaatctcg tcccttctgc cagcaccgtt ataacttagc gcgcacacac
    13141 ttaaaaagaa ctattttggc gctgccagaa agcaacgtta ttcatgccgg gtatggtagc
    13201 tatgccgtga ttgaggtgga cttagacgga ggagataagg cattttactt tgttgcgttc
    13261 agggctttca gggaaaagaa aaaactccgt ttgcatgtaa ctagcgctta tcccatttct
    13321 gaaaaacaga aaggtaaatc agtgaaattt ttcaccattg cctacaactt attgagaaat
    13381 aagcagcttc ctcagccctc aaaataacaa aacccacctt aaggtgggtt tcgccagaga
    13441 attatctctg gtattcagaa cgccattacc ggactttgcc ttgaccttgc gataatcgca
    13501 ggttgcggga tgtctgaatt tcttcagtct gctgcatcct ggaagatgag aacatgtgtt
    13561 cttattttcg tctctatcat agttgagtat ttactctctt acaatcagat ctctttcatt
    13621 gctcaacagg cgatggcttc agactttgca ttacggaatt tttaagaaag gcagggcgaa
    13681 acgaggaaga agcttttcgc cctggacaac attcctgctg acggcactac cataaaaaaa
    13741 cagaacgctt tcaacaacct tgcgtttttc atcttccgta gcggctcgga ataaatttac
    13801 gttgttacat tctgtctcat ggcctttgcg aggcgtgtcc cagatagctg tcccagtctt
    13861 tccataccgg ctgcaaaccc tgagcggtta acgcggccgc aactgcttct ggtctgcgat
    13921 cgtcgtgcgg tgagaactgt tccagctcgg ggtgattgtc ggcatagcca cctggctgcg
    13981 ttttcgaaaa agcgctgacg ttattaattg ccagcggaat cacgcgatcg cgaaaccacg
    14041 gtgattcccg cgtggagagt gagagttcaa tctccggtgc aagcagtcgg aaggcgcaga
    14101 tggtttgcac taactggcgt tcatccataa tcgacgcagg ctcaatgccg ccagtacacg
    14161 ggcgcaggcg cggaaaggag acagagtaac ggctttgcca gtaatgctgt tgcagccata
    14221 gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg
    14281 cgccgaggcc tatcttatca atccccgcac gccccagccg atccggcgtt tccagccgcc
    14341 agaagaagtc ctgttttttt cctttcagat gatggcgggc ataagtcgcc tcgtgatatg
    14401 tctcctgata aaccatcacg ccatccagac caagctgctt taactcggcg tattccgtct
    14461 ccgccagcgg ttgcacttcc atttgtagtg aagagaactg ttcacgcagg gcggggagat
    14521 gacgacgaaa gtaatccatc cccactttcg cctgatgttc accagtgact aatagcagat
    14581 gttcaaagcc catctcccgt atagcggcgc tttccctggc aatatccgct tcatccagcg
    14641 ttttgcgctt gatgcgatta ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa
    14701 gattggaaag ataaagcggg acgtagaaac taactgtgtt gccaaatcgc tgacgggtca
    14761 gacgctgcgc ccgttgggcc agttgttcca gatagccact ggcggctggc gataacagcg
    14821 ccatcatatc gtcgcgggtg aattgcgagg catttagcgc ccgctctacg tcaacagccg
    14881 ttttgccgtt gatacgcagg cggatgtcat cccagtccag ttgtcgccag cgatcgctga
    14941 aggttttcat gccgatgcct ccagaaatcc agtcagcggg ctggtggcat gagcaaaatg
    15001 actgcggctg cccggtccgg actgacgtgc cagcaggcct gcttctaccg ccagacgaaa
    15061 tgccttcgcc atgttgacgg gatcgtccgc tacggcaatc gccgtattca ctaacaccgc
    15121 gtcggccccc atttccaggg cctgcgcggc atggctggga acgccgatgc cagcatcgac
    15181 aaccaccgga actgtggcct gctggataat aatctccagc atggcacggg tttccagtcc
    15241 ctgattcgag ccaatcggcg cgccgagcgg catcaccgct gcacagccga cttcttccag
    15301 acgtttacac aataccggat cggccccgca gtaaggcagc acgacaaatc cctgttgtac
    15361 cagcatttcg gcggctttca gagtttcgat gggatcgggc aacagccagc gggcgtcagg
    15421 gtgaatctct aattttaacc agtttgtgcc taacgcttca cgtgccagat gggcggcgaa
    15481 aatggcttct tccgctgttt tcgccccgga agtatttggc agcagggtca cacccgccgc
    15541 gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag
    15601 tgtcaccagc tggctgccgg aagcgcggat cgcctccacc atcagttgtg aagaagcgaa
    15661 tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac gtaacatctc
    15721 aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg atatgttgcg
    15781 tccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc gcttgtcgtt
    15841 ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc ggcgcactgc atcggttgat
    15901 cgttaaacag gatctgcatt gcttcctccg cataccgggc aaccactggc gcggcgcaac
    15961 gccaggctgc gccactggct agacttacca tcgaacagtc ggagttctcc cgcaggcgtc
    16021 tctataccgc ttaataactt aatggcttcc agtgcctgca aagtgcccat caccccgacc
    16081 accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gctcctggtt atctggccac
    16141 aggcagcggt aacacccctg ctcccagggc ggcgtcagta ccatcaactg accgccaaat
    16201 ccgaccgcgc tggcggtgat aagcggcgtg ttgagtacca cgcaggcggc attaatctcc
    16261 tggcgagtcg ccatattgtc ggtacagtcg agcaccacat cggcccgtgc aaccgcatct
    16321 tttaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatgtc gggattcaac
    16381 tgtgtcagtc gctgttggct gacctgcgat ttcgggcgat cgatatcttc agtggtaaag
    16441 aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag taccagcgtc
    16501 ccgacgccag cgcccgccag gtatagcgca gcaggtgtac ccagcccgcc aagaccgata
    16561 atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa gagcgatatc gtcgagcagg
    16621 atttggcggc tataacgcat aaaatcacgg tcattcatcg ccaactcctg caatttccag
    16681 caactgtgcc gttgccaaac gccagtctgc ggcttgagta atggcgctga cgacggcgat
    16741 actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc
    16801 cacggtggga taatccgcca gtcgctcaac atgccgtgcc agctgttcca gcccctgcgg
    16861 tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga tataagaggg
    16921 gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt gtcgaaacac ccagccgcag
    16981 gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac
    17041 gccatacgcc tgatgcttga tcgcgagccg ccagtaatcg ttgataaaca atcgcgcgtt
    17101 atagcggcgg cccagcgcaa ttgccgccac gacatcggct tccacctctt catcgcgccg
    17161 atctttgatg cgtagctgga gagtacgtac gcctgcatcc aacagacgtt cgatccactg
    17221 tacgctgtcc accactgggt acagtcctaa acgaaaaggt acaggaggaa aatcaggctg
    17281 atacatcacg cttcctcctt acgcaggtag atttctccgc ctctggcacg gaagttctcc
    17341 gacatatccg ccattcccat ttcaatagtt tgcgtggcgg cgtaatcacg cacttcctgg
    17401 ctgattttca tcgagcagaa tttcggccca cacatggagc aaaaatgggc gactttacct
    17461 gactcttgcg gcagggtttc atcgtgataa gcgcgggcgg taaacgggtc gagggccaga
    17521 ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt atcgcgaatt
    17581 tgcgcgcccg gatgcccttt cgccaggtca gcggcgtggg cggcaatctt ataggtgata
    17641 agcccctgct taacatcttc tttattaggc agacccagat gctcttttgg cgttacgtaa
    17701 cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga cgtgaagtgg
    17761 tcatagcccg gcgcaatatc ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg
    17821 cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac gtggcctggg
    17881 ccttcaatca tcacctgcac gtcatattcc caggcaattt tggtcagttc gcccagcgta
    17941 tgcagctcgg caaactgcgc ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg
    18001 tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc gcggaagtgt
    18061 tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat cgaaccgccg
    18121 cgagagacga taccggtcag gcgtttcgcg gttatcggca catagcgcag cagtacgccc
    18181 gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac
    18241 gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc
    18301 actgtaccga tcggcaccgg gctgttacgc aaaatccact cgcgggtttc gtgaatatag
    18361 cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca taccagcttt
    18421 tccacttctt cttcgatgga agaggtgacc gccgagttgc cgatattggc gttaactttt
    18481 accaggaaat tgcgaccaat aatcatcggc tccgattccg gatgattaat gttggccggg
    18541 ataatcgcac gtccggcagc aacttcatca cggacaaatt ccgcagtgat attttccggc
    18601 agatgtgcgc caaagctcat tcccggatgc tggtggcgta aaacctcgct acggatgcgc
    18661 tcgcggccca tattctcgcg gatggcgatg aattccattt ccggcgtgat gatgccctgg
    18721 cgggcgtagt gcagttgggt gacacggcgt cctgctttgg cgcgttttgg tgttagtacg
    18781 ccgctaaaac gcagttcgtc gaggccatca tctgccagcc gcgctttagt gtaatcggaa
    18841 ctgcgcacgg taagttcttc ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt
    18901 gccagccctt gctgcacgtt aatggcaatc tgcggatcac catacgggcc ggaggtgtcg
    18961 tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt ctttgctacc gccaattagc
    19021 gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cggggtgtgt gccagtgata
    19081 taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc
    19141 cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc caaaaagtta
    19201 aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcagg ggcgatgcga
    19261 tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc gcggatcccg
    19321 aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccgc aaatggggca
    19381 ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc
    19441 gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc
    19501 gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag
    19561 actggaatcg tagtaatcca taatctgttg agtattggct tcgagttgcg gccaaatctt
    19621 cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact
    19681 gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc
    19741 gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac
    19801 gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg
    19861 ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac
    19921 tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag
    19981 agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca
    20041 aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt
    20101 gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca
    20161 gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt
    20221 tcaactggcc ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg
    20281 ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg
    20341 ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga
    20401 ttcgatcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg
    20461 attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag
    20521 cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc
    20581 tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga
    20641 attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac
    20701 cgtagcgcgc cgtctgatag aagatacggt ggcgatgtgt cgggcagagt atgagtgatg
    20761 atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg
    20821 ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca
    20881 ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc
    20941 gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc
    21001 gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg
    21061 gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga
    21121 cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt
    21181 gcgtaccatt cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc
    21241 gtggacgctg gcgacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa
    21301 aaaaatgatg tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag
    21361 cgtcactttg tatctgaatg cgcagattaa agccggtgct caggctgtga tgattttcga
    21421 cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgcttt attacatgca
    21481 taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt
    21541 taccaaaggc ggcggacagt ggctggaagc catggcagaa accggttgcg atgcgctggg
    21601 cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt
    21661 gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt
    21721 agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg
    21781 cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact
    21841 gtctgaacag tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca
    21901 aatcgaactg gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct
    21961 gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt
    22021 gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac
    22081 catgccttac attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg
    22141 ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc
    22201 tcgccgtctt ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt
    22261 ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc
    22321 cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa
    22381 cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca
    22441 acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc
    22501 ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga
    22561 taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc gtctggagcg
    22621 cctggaaagc tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa
    22681 ttacgccatg ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgcattct
    22741 cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact
    22801 ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc
    22861 ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga gtggtgaaac
    22921 gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat
    22981 gactcaggct ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca
    23041 agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat
    23101 cgagctgata aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat
    23161 aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt
    23221 cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg
    23281 cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat
    23341 aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa
    23401 agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac
    23461 tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa
    23521 ccaccgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc
    23581 agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg
    23641 cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg
    23701 atcattgatg ctggcaggtg cgctgtttct cactgcctgt agccacaact cttcacttcc
    23761 tccctttacc gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga
    23821 cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac
    23881 gacgcgagag tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag
    23941 caaaccgccg gtgaacattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca
    24001 acgtgaatcc gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta
    24061 tcgtgctgct cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg
    24121 ccaggggcgc tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa
    24181 acctgattta gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt
    24241 tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa
    24301 ctctgatttc tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata
    24361 cccatatgac cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga
    24421 atacccgctt cagccatcgc gatatcccgt ttcacccgta actcatctaa cgactgacgc
    24481 aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct
    24541 aacagggcat tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga
    24601 gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg
    24661 gcattttgct gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc
    24721 attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct
    24781 ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg
    24841 acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca
    24901 tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga
    24961 tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc
    25021 aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg
    25081 ggctggtagg gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg
    25141 ccagacaaac gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc
    25201 gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg
    25261 ccgggcagcc tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc
    25321 atagcaactc cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac
    25381 atctgggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag cctgttctgg
    25441 aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc
    25501 agcagtatgc cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga
    25561 gtgccgaaga tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct
    25621 tgctggcaag cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc
    25681 tacaagatga aatgaagcgc aaagagaagc tagtggcgct ggggcatctt gcggcaggcg
    25741 ttgcccacga aatccgtaac ccactttcct cgattaaagg gctggcgaaa tactttgccg
    25801 aacgcgcgcc agcaggggga gaagcgcatc aattggcgca ggtgatggcg aaagaagccg
    25861 accgtttaaa ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt
    25921 tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta agccaggatg
    25981 caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg
    26041 ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga
    26101 ttggtcagca tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca
    26161 gcgttaccga cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt
    26221 acttcaccac caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg
    26281 aacaacacgg tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc
    26341 tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga
    26401 tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg
    26461 ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga
    26521 acaggttttt gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac
    26581 gctgaaagag atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc
    26641 cagcgtcgag acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc
    26701 gctggatttc gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat
    26761 tgatgctgaa acacctgcgg tgactgccag ccagttcggt atggtcggta aaagctcggc
    26821 gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cggtactgat
    26881 ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc
    26941 acgtagcgaa aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga
    27001 atctgaattg ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg
    27061 gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc
    27121 gatgatgcag gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag
    27181 caaccagact atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc
    27241 agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat
    27301 tgaagtacca tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct
    27361 gcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga
    27421 tctgttgatt cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg
    27481 ggcagtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc tggcgattgc
    27541 cagtacgccg atcccgctgg ggcaaagtca ggatattcag ccgctggtgg aagtggaaaa
    27601 agaggtgatt ctggcggcac tggagaaaac gggcggcaac aaaactgaag ccgcccgtca
    27661 gttagggatc acgcgtaaaa cgttattggc aaaactgtcg cgttagttct gctcgcgttc
    27721 gatagcacgc cagccgatat ctttccggca gaagcagtcg tcccagtgga tatcggtcat
    27781 taaggcatag gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg tgacgcacag
    27841 tacgcgcccg ccgctggtca ctacctgctc gttatccgcc agtttcgtgc ccgcgtggaa
    27901 cactttgccg cctgccactt cttccagcgg caggccgtgg atcacgtcac cggtgcggta
    27961 atcacccgga tatccacccg cagccatcac cacgccgaga gaagcgcgtt catcccactc
    28021 tgacgttttc tcgtccagtt tgccttcaca ggccgccagg cagagctcaa ccagatcgga
    28081 cttcatgcgc agcataatcg gctgggtttc tggatcgcca aagcggcagt taaattcgat
    28141 aaccttcgga ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac cggtgtaggt
    28201 gttgccttcc gccgccatgc ctttcacggt tggccagatg atacgttcca tggtgcgctg
    28261 atgaacttca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc ccgtgtttgg
    28321 tccggtatct ttatcgccta cgcgtttgtg atcctggctg gtggccatcg gcagcacatg
    28381 ctcgccgtct accatcacga taaagctcgc ttcttcgcca tcgaggaact cttcgataac
    28441 gatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa cagccgcttc
    28501 cgcttcttcc agcgtcatcg ccacgataac gcctttcccg gcagccagac cgtccgcttt
    28561 aatgacgatt ggcgcgcctt tctcacgcag atacgccagc gcaggttcta cctcggtgaa
    28621 gttctggtat tccgccgtag ggatcttatg gcgggccagg aaatctttgg taaacgcttt
    28681 tgagccttcc agttgggccg cacctgcggt tgggccgaag attttcagcc cggcggcgcg
    28741 gaaggtatcg accacgcctt tcaccagcgg cgcttccggg ccgacgatgg tcagatcaat
    28801 cttttcgttt tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccaa tagcaacgtt
    28861 ttgcagcgcg ggttccagtg cagtgcctgc attacccgga gcaacaaaaa cagtctcaac
    28921 cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt taccaatcac
    28981 taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt gaagagcatc
    29041 gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggatgga accgccaggc
    29101 tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg gaacgggaag
    29161 aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcggc agctttaata
    29221 ccggcgattt tcgcggagta cacgcggctc atctggcccg cgccaatacc gatggtcata
    29281 ttgtttttgg catagacgat agcattggat ttcacgaact tcgccacttt ccagcagaac
    29341 agcgcatcgc gcagttcctg ttcggtcggc tgacgttggg tgacgacgcg cagttcttcc
    29401 gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac gcgtttgaaa
    29461 tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac gttctgcttg
    29521 gcggcggtga ttttcagggc ttcttcgctg gcggaaggcg caataatcac ttcgacaaac
    29581 tgacgagaaa tgatggcctg tgcggtttcc gcatctagct cgcggttaaa ggcaatgatg
    29641 ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc aagaatggaa
    29701 ttgccgatag ctacaccgca agggttggcg tgcttcacaa tcacacatgc cggctcggcg
    29761 aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata agagagggct
    29821 ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc ttctatatag
    29881 aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt aatgaagttc
    29941 aggttcagcg tgcgtgggaa gcgaccagcg gcttctttgc tttcaccgtg gtacgccgga
    30001 accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg ttcgaaggct
    30061 ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc atccatctct
    30121 ttaataatgg cgtcgtagtc gctgctcttc accacgattg cgacatcttt atggttctta
    30181 gcggcggagc gcaccatggt cgggccgccg atatcgatgt tctcaaccgc atcttccagc
    30241 gagcaacctt cacgggccac ggtctgggcg aacggataca ggttaacaac caccatatcg
    30301 ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg gcccagaatg
    30361 ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc cgggaaaccg
    30421 gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg ggcagtgccc
    30481 cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc gacgataccg
    30541 gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg catggtaaat
    30601 cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa actcagctaa
    30661 cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt ttgcgcaacg
    30721 ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggttac cgtgggaaaa
    30781 atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta taaagcgggg
    30841 ttttgctggg gaatgcagca gtcagttatt tttctgcaat ttttctattg cggcctgcgg
    30901 agaactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag
    30961 ccggttcggt tgaagagaaa aatcctgaaa ttcatggttg actctgaaag aggaaagcgt
    31021 aatatacgcc acctcgcgac agtgagctga aagccgcgtc gcaactgctc tttaacaatt
    31081 tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca agacgaaaaa
    31141 tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt ctttgagcat
    31201 caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg gcaggcctaa
    31261 cacatgcaag tcgaacggta acaggaaaca gcttgctgtt t
//
LOCUS       DAPPNJ010000034        31298 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000034 DAPPNJ010000000
VERSION     DAPPNJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.034"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            28..153
                     /locus_tag="RPM73_001238"
     ncRNA           28..153
                     /ncRNA_class="other"
                     /locus_tag="RPM73_001238"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            402..1445
                     /gene="nadA"
                     /locus_tag="RPM73_001239"
     CDS             402..1445
                     /gene="nadA"
                     /locus_tag="RPM73_001239"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDX6519616.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1483..2202
                     /gene="pnuC"
                     /locus_tag="RPM73_001240"
     CDS             1483..2202
                     /gene="pnuC"
                     /locus_tag="RPM73_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDX6519617.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2199..3140)
                     /gene="zitB"
                     /locus_tag="RPM73_001241"
     CDS             complement(2199..3140)
                     /gene="zitB"
                     /locus_tag="RPM73_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDX6519618.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3254..3634)
                     /locus_tag="RPM73_001242"
     CDS             complement(3254..3634)
                     /locus_tag="RPM73_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDX6519619.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3950..5002
                     /gene="aroG"
                     /locus_tag="RPM73_001243"
     CDS             3950..5002
                     /gene="aroG"
                     /locus_tag="RPM73_001243"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDX6519620.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5160..5912)
                     /gene="gpmA"
                     /locus_tag="RPM73_001244"
     CDS             complement(5160..5912)
                     /gene="gpmA"
                     /locus_tag="RPM73_001244"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDX6519621.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6117..7157)
                     /gene="galM"
                     /locus_tag="RPM73_001245"
     CDS             complement(6117..7157)
                     /gene="galM"
                     /locus_tag="RPM73_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDX6519622.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7151..8299)
                     /gene="galK"
                     /locus_tag="RPM73_001246"
     CDS             complement(7151..8299)
                     /gene="galK"
                     /locus_tag="RPM73_001246"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDX6519623.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8303..9349)
                     /gene="galT"
                     /locus_tag="RPM73_001247"
     CDS             complement(8303..9349)
                     /gene="galT"
                     /locus_tag="RPM73_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDX6519624.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9359..10375)
                     /gene="galE"
                     /locus_tag="RPM73_001248"
     CDS             complement(9359..10375)
                     /gene="galE"
                     /locus_tag="RPM73_001248"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDX6519625.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10638..12110)
                     /gene="modF"
                     /locus_tag="RPM73_001249"
     CDS             complement(10638..12110)
                     /gene="modF"
                     /locus_tag="RPM73_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDX6519626.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPVNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVSDGDLYRYALTKIN"
     gene            complement(12178..12966)
                     /gene="modE"
                     /locus_tag="RPM73_001250"
     CDS             complement(12178..12966)
                     /gene="modE"
                     /locus_tag="RPM73_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDX6519627.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            13095..13244
                     /gene="acrZ"
                     /locus_tag="RPM73_001251"
     CDS             13095..13244
                     /gene="acrZ"
                     /locus_tag="RPM73_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDX6519628.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13411..14184
                     /gene="modA"
                     /locus_tag="RPM73_001252"
     CDS             13411..14184
                     /gene="modA"
                     /locus_tag="RPM73_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6519629.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14184..14873
                     /gene="modB"
                     /locus_tag="RPM73_001253"
     CDS             14184..14873
                     /gene="modB"
                     /locus_tag="RPM73_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDX6519630.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14876..15934
                     /gene="modC"
                     /locus_tag="RPM73_001254"
     CDS             14876..15934
                     /gene="modC"
                     /locus_tag="RPM73_001254"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDX6519631.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15935..16753)
                     /gene="ybhA"
                     /locus_tag="RPM73_001255"
     CDS             complement(15935..16753)
                     /gene="ybhA"
                     /locus_tag="RPM73_001255"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDX6519632.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16908..17903
                     /gene="pgl"
                     /locus_tag="RPM73_001256"
     CDS             16908..17903
                     /gene="pgl"
                     /locus_tag="RPM73_001256"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDX6519633.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17944..18897)
                     /locus_tag="RPM73_001257"
     CDS             complement(17944..18897)
                     /locus_tag="RPM73_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6519634.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            19081..20133
                     /locus_tag="RPM73_001258"
     CDS             19081..20133
                     /locus_tag="RPM73_001258"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDX6519635.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20209..21642
                     /locus_tag="RPM73_001259"
     CDS             20209..21642
                     /locus_tag="RPM73_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDX6519636.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKVGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21825..24086
                     /locus_tag="RPM73_001260"
     CDS             21825..24086
                     /locus_tag="RPM73_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDX6519637.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24320..25603)
                     /locus_tag="RPM73_001261"
     CDS             complement(24320..25603)
                     /locus_tag="RPM73_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDX6519638.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25755..26231)
                     /locus_tag="RPM73_001262"
     CDS             complement(25755..26231)
                     /locus_tag="RPM73_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDX6519639.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(26290..27579)
                     /gene="bioA"
                     /locus_tag="RPM73_001263"
     CDS             complement(26290..27579)
                     /gene="bioA"
                     /locus_tag="RPM73_001263"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDX6519640.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSHLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHTPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARNAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            27666..28706
                     /gene="bioB"
                     /locus_tag="RPM73_001264"
     CDS             27666..28706
                     /gene="bioB"
                     /locus_tag="RPM73_001264"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDX6519641.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28703..29857
                     /gene="bioF"
                     /locus_tag="RPM73_001265"
     CDS             28703..29857
                     /gene="bioF"
                     /locus_tag="RPM73_001265"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDX6519642.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTMADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            29844..30599
                     /gene="bioC"
                     /locus_tag="RPM73_001266"
     CDS             29844..30599
                     /gene="bioC"
                     /locus_tag="RPM73_001266"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDX6519643.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            30592..31269
                     /gene="bioD"
                     /locus_tag="RPM73_001267"
     CDS             30592..31269
                     /gene="bioD"
                     /locus_tag="RPM73_001267"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDX6519644.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         7445 a         7859 c         8293 g         7701 t
ORIGIN      
        1 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc
       61 agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
      121 cgagcgaagc gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt
      181 ttttgctatt caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta
      241 ctgtgcaagc aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca
      301 ttcgactatc ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta
      361 atgtcggttt taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc
      421 cagacacggc gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag
      481 cgtattaccg cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc
      541 actactatac cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg
      601 attctctgga aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg
      661 gggtgagatt tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc
      721 cgacacttca ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat
      781 tttgcgatgc ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa
      841 aagcgcgcgc agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatcttg
      901 atagtttggg tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa
      961 aacagacggg tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta
     1021 agactcaggc gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc
     1081 cagaatcacc acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga
     1141 tcgctgctgc gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt
     1201 tctacaaaat gcagcaggca gtgccagata aagagttact ggaagcacca accgcaggtg
     1261 agggtgcaac ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagt
     1321 ccatcgcaga ggcattagaa caggaaggaa gcaatcacga ggttcatgtt gatgaaaggc
     1381 tgcgagagag ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg
     1441 gataacgaat aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg
     1501 cagaatatcc tggtacatat accaataggg gcaggcggtt atgatctctc atggatcgaa
     1561 gcggtaggca cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc
     1621 aactacttct ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag
     1681 ctgtatgcca gcctgctatt acaggtgttt ttctttgccg cgaatattta cggttggtat
     1741 gcgtggtcgc gacaaaccag tcagaacgag gcggagttga aaattcgctg gttgccattg
     1801 ccgaaggcac tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt
     1861 atcaatccgg tgtttgcatt tttgacccgc gtggcagtca tgatcatgca agcattagga
     1921 ttacaggttg tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg
     1981 atggtgttat ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg
     2041 ttgtgggtga ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc
     2101 atgtctctgg agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc
     2161 aacagcgcac gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg
     2221 ccagacaccc cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctga
     2281 atggtggcgt gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag
     2341 gcatcgtgat cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct
     2401 accatccata catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga
     2461 cgcttcagtt cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta
     2521 tctttcaaca gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg
     2581 gggtcagcag gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc
     2641 gaacccagca ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct
     2701 tcgctgccgt gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca
     2761 attgccatca tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc
     2821 cagacaatca aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg
     2881 gtcagtctta gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg
     2941 gcgagtaggg cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc
     3001 aatgccagag aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc
     3061 acgccgaaag catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg
     3121 tgtgagtgtg agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac
     3181 atcatactgc ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg
     3241 ctattttgca gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc
     3301 catcaccggt ttgtaccttt ttattgatat ccggacagcg accatctttg cacatggtgt
     3361 tcttgtgctc ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca
     3421 gcattgtgcc accagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat
     3481 tttcacgggc gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat
     3541 tagtttgcgc tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag
     3601 tcagaaataa tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg
     3661 tcggataacc tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt
     3721 cagccaggtt atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca
     3781 gttagaaatt gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt
     3841 ccaggattaa tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga
     3901 ttggaagtat tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca
     3961 gaacgacgat ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga
     4021 aaaattcccc gctactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca
     4081 taagatcctg aaaggtaatg atgatcgcct gttggttgtg attggcccat gctcaattca
     4141 tgatcctgtc gcggcaaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa
     4201 agatgagctg gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg
     4261 gaaagggctg attaacgatc cgcatatgga taatagcttc cagatcaacg acggtctgcg
     4321 tatagcccgt aaattgctgc ttgatattaa cgacagcggt ctgccagcgg caggtgagtt
     4381 tctcgatatg atcaccccac aatatctcgc tgacctgatg agctggggcg caattggcgc
     4441 acgtaccacc gaatcgcagg tgcaccgcga actggcatca gggctttctt gtccggtcgg
     4501 cttcaaaaat ggcaccgacg gtacgattaa agtggctatc gatgccatta atgccgccgg
     4561 tgcgccgcac tgcttcctgt ccgtaacgaa atgggggcat tcggcgattg tgaataccag
     4621 cggtaacggc gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa
     4681 gcacgttgct gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat
     4741 cgatttcagc catgctaact cgtccaaaca attcaaaaag cagatggatg tttgtgctga
     4801 cgtttgccag cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca
     4861 tctggtggaa ggcaatcaga gcctcgagag cggggagccg ctggcctacg gtaagagcat
     4921 caccgatgcc tgcatcggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc
     4981 agtaaaagcg cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc
     5041 gatgaatcac cacaggcctg ataagtcgcg cagcgtcgca tcaggcaatg tgctccattg
     5101 ttagcaacaa aaatgccgac tcacttgcag tcggctttct cattttaaac gaatgacgtt
     5161 tacttcgctt taccctggtt tgcaaccgcc gctgctttcg ctgcgatctc gtcagcatta
     5221 cccagataat agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg
     5281 ccagtcggga tattaagctc aagaatctct tcttcgctca tgttatcaag atatttcacc
     5341 agcgcacgta aagagttacc gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc
     5401 ggcagaatag tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc
     5461 gtcagcggca gttctttttc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc
     5521 tcatcatctt tagtcagttc cggcggagtg actgcaaaac cacgacgcca ctgtttcacc
     5581 tgctcgtcgc catacttttc agcggtttct gctttgttca gaccctgcaa cgcaccgtag
     5641 tgacgttcgt tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc
     5701 agcacattcc acagggtatg gatagcgcgt ttcagcacag aggtgtaagc aaagtcaaag
     5761 ctgtaacctt cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca
     5821 gacagatcca cgtcgtacca accggtgaag cggttttctt tgttccactg actttcgcca
     5881 tgacgaacca gaaccagctt agttacagcc atatacttac tcctcaaatc atcttttaat
     5941 gataataatt ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata
     6001 gcgaaaatag tgtcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt
     6061 ttgctggtga tttgagcaat ataaggataa agccctcatt acgagggctt caggtgctac
     6121 tcagcaataa actgatattc cgtcaggctg gaatactctt cgccaggacg caggaagcag
     6181 tccggttgcg gccattcagg gtggtttggg ctgtccggca gaaactcgct ttccagtgcc
     6241 aggccttgcc agtcggcgta aggttcagtt cctcttgatg gcgtgccgcc gaggaagttg
     6301 ccggagtaga attgcagagc cggagcggtg gtgtagacct tcagctgcaa tttttcatct
     6361 gctgaccaga catgcgccgc cactttcttg ccatcgcctt tggcctgtaa caagaatgcg
     6421 tgatcgtaac ctttcacttt gcgctgatcg tcgtcggcaa gaaactcact ggcgatgatt
     6481 ttggcgctac ggaaatcaaa agacgttccg gcgacagatt tcaggccgtc gtgcggaatg
     6541 ccgccttcat caaccggcag atattcgtcc gccagaatct gcaacttgtg attgcgcacg
     6601 tcagactgct cgccgtcaag attgaaatag acgtgattag tcatattcac cgggcaaggt
     6661 ttatcaactg tggcgcgata agtaatagag atacggttat cgtcggtcag acgatattgc
     6721 accgtcgcgc cgagattacc cgggaagccc tgatcgccat catctgaact cagggcaaac
     6781 agcacctgac gatcgttctg gttcacaatc tgccagcgac gtttgtcgaa cccttccggg
     6841 ccgccgtgca gctggttaac gccctgactt ggcgaaagcg tcacggtttc accgtcaaag
     6901 gtataacggc tattggcgat gcggttggca taacgaccaa tagaggcccc cagaaacgca
     6961 gcctgatcct gatagcattc cgggctggca cagccgagca gcgcctcgcg gacgctgcca
     7021 tcggaaagcg gaatacgagc tgaaagtaaa gtcgcacccc agtccatcag cgtgactacc
     7081 atccctgcgt tgttacgcaa agttaacagt cggtacggct gaccatcggg tgccagtgcg
     7141 ggagtttcgt tcagcactgt cctgctcctt gtgatggttt acaaacgtaa aaagtctctt
     7201 taatacctgt ttttgcttca tattgttcag cgacagcttg ctgtacggca ggcaccagct
     7261 cttccgggat cagcgcgacg atacagccgc caaatccgcc gccggtcatg cgtacgccac
     7321 ctttgtcgcc aatcacagct ttgacgattt ctaccagagt gtcaatttgc ggcacggtga
     7381 tttcgaaatc atcgcgcata gaggcatgag attccgccat caactcgccc atacgtttca
     7441 ggtcgccttg ctccagcgcg ctggcagctt caacggtgcg ggcgttttca gtcaggatat
     7501 gccgcacgcg tttcgccacg attgggtcca gctcatgtgc aacagcgttg aactcttcaa
     7561 tggtgacatc gcgcagagct ggctgctgga agaaacgcgc accggtttcg cactgttcac
     7621 gacgggtgtt gtattcgctg ccaaccaggg tacgtttgaa gttactgttg atgatgacga
     7681 cagccacacc tttcggcatg gaaactgctt tagtccccag tgagcggcag tcaatcagca
     7741 aggcatgatc tttcttgccg agtgcggaaa ttagctgatc catgatcccg cagttacagc
     7801 caacaaactg gttttctgct tcctgaccgt taagcgcgat ttgtgcgccg tccagcggca
     7861 gatgataaag ctgctgcaat acggttccga ccgcgacttc cagtgaagcg gaagaactta
     7921 acccggcacc ctgcggcaca ttgccgctga tcaccatgtc cacgccgccg aagctgttgt
     7981 tacgcagttg cagatgtttc accacgccac gaacgtagtt cgcccattga tagttttcat
     8041 gcgcgacaat gggcgcatcg agggaaaact cgtcgagctg attttcataa tcggctgcca
     8101 ttacgcgaac tttacggtca tcgcgtggcg cacagctgat cacggtttga taatcaatcg
     8161 cgcagggcag aacgaaaccg tcgttgtagt ccgtgtgttc accaatcaaa ttcacgcggc
     8221 caggcgcctg aatggtgtga gtggcagggt agccaaatgc gttggcaaac agagattgtg
     8281 ttttttcttt cagactcatt tcttacactc cggattcgcg aaaatggata tcgctgactg
     8341 cgcgcaaacg ctctgctgcc tgttctgcgg tcaggtctcg ctgggtctct gccagcattt
     8401 cataaccaac cataaattta cgtacggtgg cggagcgcag cagaggcgga taaaagtgcg
     8461 cgtgcagctg ccagtgttga ttctcttcgc cattaaatgg tgcgccgtgc cagcccatag
     8521 agtaggggaa ggagcactgg aagaggttgt cataacgact ggtcagcttt ttcaacgcca
     8581 gcgccaaatc gctgcgctgg gcgtcggtca aatcggtgat ccgcaaaacg tgggctttgg
     8641 gcagcagtag cgtttcgaac ggccaggcag cccagtaagg cacaacggct aaccagtgtt
     8701 cggtttcgac aacggtacgg ctaccgtctg ccagctcgcg ctgaacataa tccaccagca
     8761 ttggtgattt ctgttcggca aaatattctt tttgcaggcg gtcttcgcgc tcagcttcgt
     8821 taggcaggaa gctatttgcc caaatctgtc cgtgcggatg cgggttagag cagcccatcg
     8881 ccgcaccttt gttttcaaag acctgcaccc acgggtacgt ttttcccagc tctgcggttt
     8941 gctcctgcca ggttttgacg atttccgtca atgccgcaac gctcagttct ggcaacgttt
     9001 tactgtgatc cggtgaaaag cagatcaccc ggctggtacc gcgcgcgctc tggcaacgca
     9061 tcaaaggatc gttgctttct ggcgcatctg gcgtgtcaga catcaaggcc gcaaaatcat
     9121 tagtgaaaac gtaagtcccg gtgtaatcgg ggtttttatc gcctgtcacc cgcacattac
     9181 ctgcgcagag gaagcaatct ggatcgtgcg caggtaacac ctgtttggct ggcgtttcct
     9241 gcgccccctg ccaggggcgc ttagcgcggt gcggtgaaac cagaatccat tgcccggtga
     9301 gcgggttgta gcggcgatgt ggatgatcaa cgggattaaa ttgcgtcatg gtcgttcctt
     9361 aatcgggata tccctgtgga tggcgtgact gccagtgcca ggtgtcctgc gccatttcat
     9421 cgagtgtgcg cgttacgcgc cagttcagtt cgcgatcggc tttgctggcg tccgcccagt
     9481 aggccggaag gtcgccctcg cgacgcggtg caaaatgata gttaaccggt ttgccgcagg
     9541 ctttgctgaa ggcattaacc acgtccagca cgctgctgcc tacgccagcg ccgaggttgt
     9601 agatgtgtac gcctggcttg ttcgccagtt tttccatcgc cacgacgtga ccgtccgcca
     9661 gatccattac gtggatgtaa tcgcgtacgc cagtaccatc ttcggtcgga taatcgttac
     9721 caaaaatcgc cagcgagtcg cgacggccta cagcaacctg ggcgatgtat ggcatcaggt
     9781 tattcggaat gccttgcgga tcttcaccca tatcgcccga cggatgcgcg ccaaccgggt
     9841 tgaagtagcg cagcagggca atgctccagt ccggctgggc tttttgcagg tcggtgagga
     9901 tctgttccac catcaatttg cttttgccat aagggctttg cggtgtgccg gtcgggaagc
     9961 tttcaacgta tggaattttg ggctgatcgc cataaacggt ggcggaggag ctaaaaataa
    10021 agtttttgac gttagcggcg cgcatggcgc taatcaggcg cagagtgccg ttgacattgt
    10081 tgtcgtaata ttccagcggt ttttgtaccg attcgccgac ggctttcagc ccggcgaagt
    10141 ggatcacggt gtcgatagcg tgatcgtgca gtatctcggt catcaacgct tcgttacgaa
    10201 tatcgccttc aacaaacgtt ggatgtttgc cgcctaaacg ctcgataaca ggcagtacgc
    10261 tgcgcttact gttacagagg ttatcaagaa tgatgacatc atgaccgttt tgcagtaatt
    10321 gcacgcaggt atgacttcca atgtaaccgc taccaccggt aaccagaact ctcataattc
    10381 gctccattag gcttatggta tgaaataacc atagcataac aaagatgcga aaagtgtgac
    10441 atggaataaa ttagtggaat cgtttacaca agaatttagc cgttttttat atgcgattaa
    10501 gtgattataa aacagaggat ttatgaatga ttgcgctttt tatctgaaaa aaaagacgcg
    10561 gtttcatgcc ttgatgcgtc gaactgtagg ccggagaggg ggcttacgcc acatccggca
    10621 cagtcagcat taccaactca gtttattttt gtcagcgcat agcgataaag gtcaccgtcc
    10681 gacacgaact caaggcgatg ggtaatacag gcaggcgcat cttcagcgtg gtgcgaaaca
    10741 aacaacaatt gcgtttcacc ttcgctaatc agcacatcaa caaaacggcg gataagttgg
    10801 cgattgagcg gatcaagccc ctgtagtggt tcatcgagaa taagcaacgt cggatgtttc
    10861 accagtgcgc ggacgatcag cgccagacgc tgctgtcccc aggaaagact atggaaagga
    10921 gcgtcagccg tgcgtttatc aatgccgaga atatccagcc actgctgcac cagtttttgc
    10981 tggcgatccg aaacggcctg ataaatgcca atcgaatcaa aatagccaga aagaatcaca
    11041 ttacgcacag tagtactgac ccggtaatcc agatgcaaac tactgctgac gtaaccgata
    11101 tgcttcttga tatcccagat ggtttcgccg ctgccacgac gtcgtccgaa aagcgtcaaa
    11161 tcgttgctgt aaccttgcgg atgatcgcca gtaatcaggc ttaataacgt cgattttccc
    11221 gcgccatttg gcccgacaat ttgccagtgt tcacctggat tcacctgcca gctaaggtta
    11281 ttaagaatgg ggcgatcgtt ataagaaacc acgccattgt tcagcacaat gcgcggttcg
    11341 ttgacgggta aggcgtgacg tgctgaaggt tcatccggct ccggcagttg cacaccttca
    11401 agctgttcgc tatgcgccag ttgcgcgacg agtgcttgct ggagcagttc ctctttagcg
    11461 cccgtttccg ctaacgtaca atccgccagc acgccagcaa actggacaaa ttccgggatc
    11521 tcatcgaagc gattgagcac cagtaccaaa gtaataccgg actgatgtaa cgaggcgagt
    11581 agctcagcca gctgctgacg tgaggcaaca tccaggccat cgaacggctc atcaagaatc
    11641 aacaagtcag gctccgacat cagcgcctga cacagcaggg tttttcgcgt ctcgccagtg
    11701 gaaaggtatt taaagcgtcg gtcgaggagg gcggtaatac cgaactgctg cgccagttgc
    11761 atgcaacgcg gtgcatcctt tacttcatcc tgaataatct cagccgtagt gcgtccggtg
    11821 tcatcttcgc cagggccgag catatcggtg ttattccgct gccattcgtc gctgacgagt
    11881 ttttgcaatt gctcgaagga gagacgagtg atgtgggaaa actggctttg ccgttcacct
    11941 ttcaaaagcg gaagttcccc cgccagcgcg cgggccagcg ccgatttccc gcttccattc
    12001 gaaccgacaa acgcccaact atcacccgcg tttaacgtta gctgaggcaa ttgcagcgtt
    12061 tttgtgtcgc taagacgaaa cgtgccttgc aaaatttgca acgatgacat tttatatccc
    12121 attttgtgca gcgattactg acagggatac gtgtttcata acaaattgtc aacacgctta
    12181 gcacagcgtg gcgataatca cgctgtcggc attaaagtag gccgtgacat tctgcccttg
    12241 ctgaagagaa gtcgcttcat ttaccggcac tgtggcgcac agtgtttgcc cgtcgggtag
    12301 cgccattaat acttcgcact gctctgcgcc gcgctcaata tgactaataa tacccggtaa
    12361 ttggttgtca gcgttttgcg cgaccgcctc gtcctgagta atacctaccc acggcgcttt
    12421 tagcaatatc aacacttctt tgccttcatc cagccccaga cgcgcgccgc tttgtgcggt
    12481 aattgcgact ttcaggcgtg tttttccgtc agccagtaag acatcaacat gctgttgaac
    12541 gtcatcatga tcgcgggcgg tgatggtgcc gaaccactgg ttacgggcgc tggtttgcag
    12601 tgaaaaacgt gagatcgcgg ccagcaggct gttcagcggc agggcgtcat cgtcacttaa
    12661 cacatcaaag gctttttgct ggatttgcgc cagtaagtca tagagctgaa tcagtcgctg
    12721 accatagcgg gtcagtactg cgccgccgcc acctttaccg cctgttgcgc gctcgaccag
    12781 aatatgctca cttaactgat tcatctcgtt aatggcatcc caggcgcttt tatagctgat
    12841 accggcatct ttcgctccct ggctaatgga accggaaagc gcaatgtgtt ttagtagcga
    12901 aatccggcgc gggtcggcga ataatttttg ttggagctta agggtgagaa ggatttcggc
    12961 ctgcataaca atgtcctggc aaaagtctta ttgtgacgga aaacgaacgc cacgcaaagc
    13021 tgaccgcaca aaaggggagt gcttttctgt gcttagcggt tagaatagtc tcatgactat
    13081 atctggagtt gaccatgtta gagttattaa aaagtctggt attcgccgta atcatggtac
    13141 ctgtcgtgat ggccatcatc ctgggtctga tttacggtct tggtgaagta ttcaacatct
    13201 tttctggtgt tggtaaaaaa gaccagcccg gacaaaatca ttgattccct gaatgcccgc
    13261 ttagtcgggc attttctttt tctcaacttc ctgcttttcc tgccgatatt ttttcttatc
    13321 tacctcacaa aggttagcaa taactgctgg gaaaattctg agctagccgt tatattgtcg
    13381 cctacataac gttacattaa ggggttacca atggctcgta aatggttgaa cttgtttgcc
    13441 ggggcggcac tctctttcgc tgttgctggc aatacactgg cagatgaagg gaaaatcacg
    13501 gtgttcgccg ccgcatcact gactaacgca atgcaggaca ttgctacgca gtataaaaaa
    13561 gaaaaaggcg tggatgtggt ttcttctttc gcttcgtcat ctactctcgc ccgtcagatt
    13621 gaagcgggtg cgcctgcgga tctgtttatt tctgccgatc agaaatggat ggattatgcg
    13681 gttgataaaa aagcgatcga tactgctacg cgtcagacac tgctcggcaa tagcctggtc
    13741 gttgtagcac cgaaagccag cgagcagaaa gatttcacca tcgacagcaa aaccaactgg
    13801 acttcgctgc tgaatggcgg tcgtctggcg gttggcgatc cggaacatgt tcccgctggc
    13861 atttatgcaa aagaagcact gcaaaaactg ggcgcatggg atacgctctc tccgaaactg
    13921 gctccggcgg aagatgttcg tggcgcgctg gcgttggtcg aacgcaatga agcgccgctg
    13981 ggcatagttt acggttctga cgcagttgcc agcaaagggg taaaagtggt cgccaccttc
    14041 ccggaagatt cgcataaaaa agtggaatac ccggttgctg ttgtggaagg gcataacaat
    14101 gcgacagtga aagcatttta tgattatttg aaagggccgc aggctgcgga aatctttaaa
    14161 cgttacggat ttacaaccaa gtaatgatac tgaccgatcc agaatggcag gcagttttat
    14221 taagcctgaa agtttcttcc ctggctgtgc tgtttagcct gccgtttggg atcttttttg
    14281 cctggttact ggtgcgttgc acgtttccgg gcaaagctct gctcgacagc gtactgcatc
    14341 taccgctggt gttaccgccc gtggtcgtcg gttacttatt attagtttcg atgggacggc
    14401 gcgggtttat cggtgaacgt ctgtatgact ggtttggcat taccttcgcc tttagctggc
    14461 gcggcgcggt tctcgctgcc gccgtcatgt cgtttccgct gatggtgcgg gcaattcgtc
    14521 tggcgctgga aggggttgat gtcaaactgg aacaggccgc aagaacactg ggggccgggc
    14581 gctggcgcgt tttctttact atcacgatac cgctgacctt accgggaatt attgttggta
    14641 cggtactggc ttttgctcgt tctctcggtg aatttggcgc aaccatcacc tttgtgtcga
    14701 acattcctgg tgaaacgcga accattcctt ctgccatgta taccctgatt cagacccccg
    14761 gcggggaaag tggcgcagcg agactgtgca ttatttctat tgcgctggcg atgatctccc
    14821 tgttgatttc agaatggctg gccagaatca gccgtgaacg ggcggggcgc taatcatgct
    14881 ggaactgaat ttttcccaga cgttgggcaa ccattgcctg actattaatg aaacgctgcc
    14941 cgccaatggc atcactgcta tctttggcgt ctccggtgcc ggaaaaactt cgctgattaa
    15001 cgccatcagt ggactgacgc gcccgcaaaa agggaggatt gtcctcaatg gtcgggtact
    15061 aaatgatgcc gaaaaaggta tctgcctgac gccagaaaag cgtcgcgttg gctatgtttt
    15121 tcaggatgcg cggctgtttc cgcattacaa agtgcgtggc aatctgcgct acggcatggc
    15181 gaaaagcatg gtcgatcagt tcgataagct ggtggcgctt ttaggcattg aaccgttgct
    15241 tgaccgttta ccaggcagcc tgtccggagg cgaaaaacag cgcgtagcga ttgggcgggc
    15301 tttgctgacg gcaccagaat tgctgttgct ggatgaaccg ctggcgtcac tggatattcc
    15361 gcgtaaacgc gaactgttgc cttatctgca acgtctgacg cgggaaatca acatcccgat
    15421 gttgtatgtc agccattcgc tggatgagat cctccatttg gcagacagag tgatggtact
    15481 ggaaaacggt caggtgaaag cctttggcgc actggaagaa gtctggggta gtagcgtgat
    15541 gaatccgtgg ctgccgaaag agcaacaaag tagcattctg aaagtgacgg tgcttgagca
    15601 tcatccgcat tacgcgatga ccgcgctggc gctgggcgat cagcatttgt gggtcaataa
    15661 gctggacgaa ccgctgcaag ctgcgctacg tatccgcatt caggcttccg atgtttctct
    15721 ggttttacaa ccgccgcagc aaaccagcat tcgtaacgta ttgcgggcaa aagttgttaa
    15781 tagttatgac gacaacggcc aggtggaagt ggaactggaa gtcggaggta aaacgctgtg
    15841 ggcgcgtatc agcccgtggg ccagggatga actggtgatc aaacctggcc tgtggctgta
    15901 cgcgcaaatt aaaagtgtgt cgataaccgc ctgattaaat caggtggcta taaatgaact
    15961 gggcaatgct gtcggtggtg ttatcaccaa tcacaatgtt ggcgcgcgct tttaccgcgt
    16021 catcggcgtt gcccatcgcc acgcctgtac cagcggcctc cagcatactg atatcattaa
    16081 agttatcgcc gaacgccacg acgttttcca tcgaccagcc ttgcgcctca acccatttcg
    16141 tcaaacgttt acctttgctg ttgccgccgc gtgcaatatc aacctgatcg tgccaggacc
    16201 attcacactc cagtcccagt tcatgttcga catgcttacc aaaatgctgc aattgcggca
    16261 ggtcatcgtg cgtcagggcg aacttccata cggcgttaac ttgttgcgcc gtttcagcca
    16321 gagaagcgac ttgtgtgaaa gtcggacgct gttccggcgg cagggtttgc gcccagttag
    16381 acgtgcgaat gacatgcccg gtcgggtgct catagaccat tgcatcatcc acatacatca
    16441 gaccgtgaat gtggtgttca ttcagcatct caatgagttg cagggcttta ttaacgggca
    16501 ttgggtccgc ttccagcacg gtttttgcat gataatcata caaataggtg ccattacagc
    16561 aaatagcagg tgtatccagc gccagcgcct gataaaaagg atgaatagcg acgtgatggc
    16621 gacctgtgac gatgattaat tgatagcctg cttcgcgagc gcgggccagg gcttctatcg
    16681 atgaaggaag cagggtcttt ttcggggtca ataaggtgcc gtctaagtcg agagcaatca
    16741 cgcgtgtggt catggcgtat tccagattaa ggttaagaat tttcgtctgc gcgaatggta
    16801 caccgatacc actatcggac aaaattctgc gttttaattc agcattcacc gccaaaagcg
    16861 actaatttta gctgttacag tcagttgcta aatgcaaagg agcattcatg aagcaaacag
    16921 tttatatcgc cagccctgag agccagcaaa ttcacgtctg gaacctgaat catgaaggcg
    16981 cactgacgct gacacaggtt gtcgatgtgc cggggcaggt gcagccgatg gtggtcagcc
    17041 cggacaaacg ttatctctat gttggtgttc gccctgagtt tcgcgtcctg gcgtatcgta
    17101 tcgccccgga cgatggcgca ctgacctttg ccgcagagtc tgcgctgccg ggtagtccga
    17161 cgcatatttc caccgatcac caggggcagt ttgtctttgt aggttcttac aatgcgggta
    17221 acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg cgtcgtcgat gtggtcgagg
    17281 ggctggacgg ttgccattcc gccaatatct caccggacaa ccgtacgctg tgggttccgg
    17341 cattaaagca ggatcgcatt tgcctgttta cggtcagcga tgatggtcat ctcgtggcgc
    17401 aggaccctgc ggaagtgacc accgttgaag gggccggccc gcgtcatatg gtattccatc
    17461 caaacgaaca atatgcgtat tgcgtcaatg agttaaacag ctcagtggat gtctgggaac
    17521 tgaaagatcc gcacggtaat atcgaatgtg tccagacgct ggatatgatg ccggaaaact
    17581 tctccgacac ccgttgggcg gctgatattc atatcacccc ggatggtcgc catttatacg
    17641 cctgcgaccg taccgccagc ctgattaccg ttttcagcgt ttcggaagat ggcagcgtgt
    17701 tgagtaaaga aggcttccag ccaacggaaa cccagccgcg cggcttcaat gttgatcaca
    17761 gcggcaagta tctgattgcc gccgggcaaa aatctcacca catctcggta tacgaaattg
    17821 ttggcgagca gggactactg catgaaaaag gccgttatgc ggtcgggcag ggaccaatgt
    17881 gggtggtggt taacgcacac taaccgctga tttaaccggc gcagtctctc ctgcgccggt
    17941 gtattaacct atctcctgta acgcatgtct ctggcgttcg acgatattgg tccacaaatt
    18001 gtctttatcg tcagtccaca aattaatcag caaggcaaaa aagcgttctg ctgccggaga
    18061 aagcacggca tctttgcggc gaataatccc caatgtccga cgtataacgg gttccaccag
    18121 tgggatacca ataatcgaag aatagggcgc atggggcatt gccagaccag gaagcgccga
    18181 aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa agatgacgca cttcgtaaaa
    18241 ccagtccagc ttccagggct tgtcggctag ctgttgttct atcaacaatc ggttgccgct
    18301 ggaggaacgt acgccaatca ttttgtaacc cactaactcc tgccattcta caagctgctt
    18361 tttggccaat ggatggtcac gccgacaggc cagcacgaat ggctcgttga ccagtggggt
    18421 aaaatcaatg gatgaatttg tgacgttgtt catgtttatg ccaaagtctg attcgttaca
    18481 cagcaccgat tccatgcaat tattcgtgcc ttgttccaga atccgcactt taatattggg
    18541 atacagctca ttaaatttac cgattgccag cggtaaaaaa taaaatactg cggtcggaat
    18601 acacgctaac gtcaccatac cacgatgata agcattcata tcacgaatat taaaaagcgt
    18661 ttcatcgaat ttctttatta attcccgcgc ctccgggagc aacctttttc ctgcttttgt
    18721 taacgtaacc ttgcgagttg tacgctcaaa tagttgaata tgtaaatctt cctccatctt
    18781 ttttattctt cgcgttaatg caggttgcgt aatattgagt aatttagcgg cattgttaaa
    18841 tgaactggac tctgccagta tgacaaatgc cttcatactt gataattcat gcttcattat
    18901 tactccggaa aatggaagcg acgattttgg gtggctggcc gttaaaaatt ttaactgcat
    18961 ttagccaact taaattaatg aaaaaatgtt attaatcgtt gagctaaagt cattagagat
    19021 gctttgccct taatgtaacc atatcgcaat aagttatgtt tttaaattga gggcattatt
    19081 atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct cgaggggcgc gttcttgtta
    19141 gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa tattgatggc aattatgggt
    19201 tccggtaacg atctggaaat tgacggtatt ggcggcggta atccgctgac cagtaaagtc
    19261 gccattatta gccgttccag cgatccgcgt gctgatgtcg attatctgtt tgctcaagtt
    19321 attgtccatg agcaacgtgt cgataccacg cctaattgtg gcaatatgct gtctggtgtt
    19381 ggggcattcg ccattgaaaa tggtttgatt gcagcgactt caccagttac ccgcgtacgt
    19441 atccgcaacg tcaatactgg tacgttcatc gaagctgatg tgcaaacgcc aaatggtgtt
    19501 gtcgagtacg agggtagtgc cagaattgac ggcgtaccgg gtactgccgc accggttgcg
    19561 ctcactttcc tgaatgccgc tggaaccaaa accggaaaag ttttcccgac tgataatcag
    19621 attgattatt ttgacgatgt cccggtgacc tgtatcgata tggcgatgcc agtcgtcatt
    19681 attccggctg aatatctggg aaaaacaggt tatgaattac cggcggaact ggatgccgac
    19741 aaagcattat tagcccgcat tgaatctatc cgtctacaag cgggtaaagc aatgggctta
    19801 ggtgatgtca gtaatatggt tatccctaaa cctgtgctta tttctccagc gcagaaaggc
    19861 ggggcaatta atgtgcgtta ttttatgccg cattcttgcc atcgcgcgct ggcgataacc
    19921 ggtgctattg ctatttccag tagttgtgca ttggaaggca ccgtcacccg acaaatcgtc
    19981 ccttctgtag gatacggcaa tatcaatatt gaacacccca gtggtgcgct cgacgttcat
    20041 ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat ctgttattcg gacgaccaga
    20101 aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt cgttgtcagg ataaggacaa
    20161 tcaataaagg acttctgtat gagtcataca gaaagaacag gattttaaat gaataagaaa
    20221 tcgttatgga agctaattct gatattagcg atcccatgta ttattggttt tatgccagct
    20281 ccggcaggat taagcgaact ggcgtgggtg ctttttggta tttacctggc ggccattgtg
    20341 gggctggtta tcaagccttt cccggaacct gtcgtactgt taattgccgt tgctgcctcc
    20401 atggtggtgg tcggtaactt atccgacggt gcgtttaaga ccaccgccgt attaagcggt
    20461 tactcttcag gtactacctg gctggtgttc tcggcgttta ccttaagcgc cgcatttgtg
    20521 accaccggtt taggtaaacg tattgcctat ctgctgattg gtaaaatcgg taacaccacg
    20581 ctgggtctgg gttacgttac ggtattcctc gatctggtac tggctccggc aacaccgtct
    20641 aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca acagcgtggc ggtggctttg
    20701 gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt acctgatgat gtccatttac
    20761 atggtcacca aaaccaccag ctatatgttc tttaccgcaa tggcggggaa cattctggcg
    20821 ctgaaaatga tcaacgacat tctgcacctg caaattagct ggggtggatg ggcactggcc
    20881 gccggattgc cgggcatcat tatgctgctg gtcaccccac tggtgattta taccatgtat
    20941 ccgccagaaa ttaagaaggt ggataataaa accatcgcta aagtgggcct tgccgaactg
    21001 ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct ttgtgctggc gctgctgggc
    21061 tggattttca gtaagtctct gggggttgat gaatccaccg tggcaatcgt tgttatggcg
    21121 accatgctgc tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg
    21181 aataccttaa tctggtacgg cggtattatc ggattaagct ccttattatc gaaagttaaa
    21241 ttcttcgaat ggttagctga agtctttaaa aataacctgg catttgatgg tcacggtaac
    21301 gttgctttct tcgttattat tttcctcagc attatcgtgc gttatttctt cgcttccggt
    21361 agtgcctata tcgttgctat gttaccggta tttgccatgc tggcgaacgt ctccggcgca
    21421 ccgttaatgt taaccgcgct ggcactgttg ttctccaact cctatggcgg catggttact
    21481 cactatggcg gcgcggcagg tccggtcatc tttggcgtgg gttacaacga tattaaatcc
    21541 tggtggttgg tcggtgcggt actgacgata ttaaccttcc tggtgcatat caccctcggc
    21601 gtgtggtggt ggaatatgct gatcggctgg aacatgctgt aaatatactc gtcatacttc
    21661 aagttgcatg tgctgcgttt gcgttcgctc accccagtca cttacttatg taagctcctg
    21721 gggattcact cacttgtcgc cttcctgcaa ctcgaattat ttagagtata tccatttatt
    21781 atctttctgc gcacttcacg gtgcgcagat atctggagca tttgatgatc aagttatctg
    21841 aaaaaggcgt gtttctcgcc agtaataacg aaataattgc cgaagaacat ttcaccggcg
    21901 aaattaaaaa agaagaagcc aaaaaaggca ctattgcctg gtctattctc tcttctcata
    21961 atacgtccgg aaatatggat aaacttaaaa ttaagtttga ttcattagcc tctcacgata
    22021 ttacctttgt tggtattgta cagaccgcta aagcgtccgg aatggaacgt ttcccgctgc
    22081 cgtatgtgct gaccaactgc cataactcac tctgcgccgt cggcggcacc attaacggtg
    22141 atgaccatgt ttttggttta tcggcggccc agcgttatgg cggtattttt gtgcctccgc
    22201 acattgcggt catccatcaa tatatgcgtg agatgatggc aggcggcggc aaaatgatcc
    22261 tcgggtcaga cagccacacc cgttacggtg cattagggac aatggcagtc ggtgagggtg
    22321 gcggtgagtt ggtaaaacag ctgcttaatg acacctggga tatcgactat ccgggcgtgg
    22381 ttgcggtgca tctgaccgga aaacctgcgc cgtatgtggg gccacaggat gtggcgctgg
    22441 ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg
    22501 gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg atgaccacgg
    22561 aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga agtgcataac tggctggcgc
    22621 tgcacggtcg cggccaggat tactgccagc ttaaccctca accgatggcg tactacgatg
    22681 gctgcatcag cgttgattta agcgccatca aaccaatgat tgcgctgccg ttccacccga
    22741 gcaacgtgta tgaaatcgac acactgaacc agaacctgac cgacattctg cgtgagattg
    22801 aaattgagtc cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg gataaagtgg
    22861 aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg ctgttctggc ggtaactacg
    22921 aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat gacaccttct
    22981 cgctggcagt ttacccgtca tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg
    23041 tagcagattt gattggcgca ggggcaatca tcagaaccgc gttctgcggc ccatgctttg
    23101 gcgcgggcga tacgccaatc aacaacggtt tgagtattcg ccacaccacg cgtaacttcc
    23161 cgaaccgcga aggctctaag ccagctaatg ggcagatgtc agcggtggcg ttgatggacg
    23221 ctcgttctat cgctgcgact gcggcaaacg gtggctattt aacctctgcc agcgaacttg
    23281 attgctggga caacgtgccg gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt
    23341 atcagggctt tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg aacattaaag
    23401 actggccgga attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc
    23461 tcgacgaagt gaccaccacc gacgaactga ttccttccgg tgaaacctct tcttatcgtt
    23521 caaatccgat tggtctggcg gagtttaccc tgtcacgccg cgatcccggt tatgttggca
    23581 gaagtaaagc gactgctgag ctggaaaatc agcgtctggc ggggaatgtc agcgagctga
    23641 cagaggtgtt tgcgcgcatt aagcagattg ctggtcagga gcatattgat ccgctgcaaa
    23701 ctgaaattgg cagcatggta tatgcggtga aaccaggcga tggttctgcg cgtgaacagg
    23761 cggcgagctg ccagcgtgtg attggcggtc tggcgaatat tgccgaagag tacgcgacta
    23821 aacgctaccg ttctaacgtc atcaactggg ggatgttacc gctgcagatg gcggaagtgc
    23881 caacctttga agtgggggat tacatttaca tccctggcat taaagcggcg ctggataatc
    23941 cgggtacgac gtttaaaggt tatgtgatcc atgaagatgc gccggtaacg gaaattacgc
    24001 tctatatgga aagtctgact gctgaagagc gcgagattat caaggcgggt agtttgatta
    24061 acttcaataa aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg cattcggcac
    24121 ttattgtcgg atgcgatgct tgcgcatctt atccgaccta cgaatcgcat cgaatctgta
    24181 ggccagataa ggcattttcg cagcatccgg cacttattgt cggatgcgat gcttgcgcat
    24241 cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta cgcagcatcc
    24301 ggcaaatagt taattgctct tacttcttcg cctctgcaac cactttacta cccacgccgc
    24361 ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt
    24421 cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc accgcatcgg
    24481 cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac
    24541 cgttggtatt ggcatcaaca tccagcgagc ggcccagttg cgccacacca tcaccgaaag
    24601 cattgaaacg gctgtttacg gcgaggaaac cgtagtaaat gttggacagc gtagccggtg
    24661 caaacacata cgcttcttgc tgagtacgtg agttcaccac gcggaattcg gtgttatcga
    24721 acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatgtag ctgttggtca
    24781 ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg ttggtgacaa
    24841 agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg tcaccatcag
    24901 tacgcagtgc caccgccgga tggttacctg catctacgct atcgcccagc gtgttttcga
    24961 tggtcagatt ttgcagttgc aggccattgt tttgtgacca gaagaccgca gagcagagaa
    25021 caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac
    25081 ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgccac
    25141 catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtaa
    25201 ttccacccgg agcggcaggg acatataccg ttccctgata ctcaccaggc atcacggcaa
    25261 tatactggcg cttgttggta cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg
    25321 tatgcgttac accttgagtg cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg
    25381 taatcgggga aggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgag
    25441 catcgaaatt ctgcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcgg
    25501 tttgatcaga aggacgttga tcgggcgggg ttgagctaca ggcggtcagc gtcacgccaa
    25561 aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat
    25621 gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaacttgag cgaaacggga
    25681 aggtaaaaag acaaaaagtt gtttttaata cctttaagtg ataccagatg gcattgcgcc
    25741 atctggcaga gtgattaact aaacatcgca gtaatcgagg cgcttgccag agagtggaaa
    25801 tgaacgttaa acccgaccat cgcgccgctg gcaccttcat cgacatcaat acgttctata
    25861 tccagcgcgt gaacggtaaa aatgtagcga tgagtttcgc ctttcggcgg tgctgcgcca
    25921 tcgtacccgg ttttaccaaa gtcggtacgc gtctgcaaaa cgccgtctgg cattgctacc
    25981 agaccagagc caaacccttg cggtaatacg cgggtatcag cgggtaagtt aacaactacc
    26041 cagtgccacc agccggagcc ggttggcgca tccgggtcat agcaggtgac gacaaaactt
    26101 ttcgttcccg caggaacatc atcccacgcc agatgcggtg aaatattatc gccatcgtaa
    26161 cccatgccgt taaagacatg acgatgcggc aacttatcgc catcgcgcag atcattactg
    26221 atgagtttca tgaaccctcc tttctagttt gcagaaagtg tagccagaaa cccacacgct
    26281 gacttcccgt tattggcaaa aaaatgtttc atcctgcacc gcacggttaa ccgctgcggt
    26341 cagacgctgc aactgttgcg ggagaataat atagggcggc atcaggtaaa tcagtttgcc
    26401 aaaaggccgg atccagacac cctgttcgac aaagaatttt tgcagcgccg ccatattcac
    26461 cggacgagtt gtttcgacta cgccgattgc ccccagcacg cgcacatcgg caaccatttc
    26521 ggcattacgg gcgggggcaa gttgctcgcg cagctgtact tcaatatccg ccacctgttg
    26581 ctgccagtcg ccagattcga gaatcgccag gctggcgttt gcagccgcgc aggccagcgg
    26641 attgcccata aaagttggcc cgtgcataaa gcagccggct tcgccgttac tgatggtttc
    26701 tgcaacctcg cgcgtggtga gtgtggcgga aagggtcatt gtgccgccgg ttaaggcttt
    26761 accgaggcac aaaatgtccg gcgcgatttc tgcatattca caggcaaaca gtttgccggt
    26821 acgaccaaat ccggtggcga tctcgtcggc aatcagcaag ataccttcgc gatcgcacat
    26881 tttgcggatt cgttttaacc attccggatg gtacatgcgc atcccgcctg cgccctggac
    26941 aatcggctca atgatcaccg ccgcgatttc atgacgatgc gccgccatca ggcgggcaaa
    27001 gcccaccata tcgcgctcat cccattcgcc atccatgcgg ctttgcgggg cgggagcaaa
    27061 caggttttct ggcaggtagc ctttccacag actgtgcatt gagttatccg gatcgcacac
    27121 cgacatcgcg ccaaaggtat cgccatgata accattgcgg aaggtcagaa aacgctggcg
    27181 cgcttcgcct ttggcttgcc agtactgcaa cgccattttc atcgccactt ccaccgctac
    27241 ggaaccggag tccgcaagaa aaacgcactc cagcggttgc ggcgtcatcg ccaccagttt
    27301 gcggcacagc tcaatggctg gcgtatgggt gataccgcca aacatcacat gcgacatggc
    27361 atcaatttgc gacttcatcg ccgcattaag ctgtgggtga ttgtagccgt ggatcgccgc
    27421 ccaccaggac gacataccgt caaccaggcg tctgccgtca gacaaaatta gctcgcaacc
    27481 ttcggcgctc gccaccggat aaaccggtag atgggaggtc atggatgtgt atgggtgcca
    27541 gatatggcgt tggtcaaagg caagatcgtc cgttgtcata atcgacttgt aaaccaaatt
    27601 gaaaagattt aggtttacaa gtctacaccg aattaacaac aaaaaacacg ttttggagaa
    27661 gccccatggc tcaccgccca cgctggacat tgtcgcaagt cacagaatta tttgaaaaac
    27721 cgttgctgga tctgctgttt gaagcgcagc aggtgcatcg tcagcatttc gatcctcgtc
    27781 aggtacaggt cagcacgttg ctgtcgatta aaaccggagc ttgtccggaa gattgcaaat
    27841 actgcccgca aagctcgcgc tacaaaaccg ggctggaagc cgagcggttg atggaagttg
    27901 aacaggtgct ggagtcggcg cgcaaagcga aagcggcagg atcgacgcgc ttctgtatgg
    27961 gcgcggcgtg gaagaatccc cacgaacgcg atatgccgta cctggaacaa atggtgcagg
    28021 gggtaaaagc gatggggctg gaggcgtgta tgacgctggg cacgttgagt gaatctcagg
    28081 cgcagcgcct cgcgaacgcc gggctggatt actacaacca caacctggac acctcgccgg
    28141 agttttacgg caatatcatc accacccgca cttatcagga acgcctcgat acgctggaaa
    28201 aagtgcgcga tgccgggatc aaagtctgtt ctggcggcat tgtgggctta ggcgaaacgg
    28261 taaaagatcg cgccggatta ttgctgcaac tggcaaacct gccgacgccg ccggaaagcg
    28321 taccaatcaa catgctggtg aaggtgaaag gcacgccgct tgccgataac gatgatgtcg
    28381 atgcctttga ttttattcgc accattgcgg tcgcgcggat catgatgcca acctcttacg
    28441 tgcgcctttc tgccggacgc gagcagatga acgaacagac tcaggcgatg tgctttatgg
    28501 caggcgcaaa ctcgattttc tacggttgca aactgctaac cacgccgaat ccggaagaag
    28561 ataaagacct gcaactgttt cgcaaactgg ggctaaatcc gcagcaaact gccgtgctgg
    28621 caggggataa cgaacaacag cagcgtctgg agcaggcact gatgaccccg gacactgacg
    28681 aatattacaa cgcggcagca ctatgagctg gcaggagaaa atcaacgcgg cgctcgatgc
    28741 gcggcgtgct gccgatgccc tgcgtcgccg ttatccagtg gcgcaaggag ccggacgctg
    28801 gctggtggcg gatgatcgcc agtatctgaa cttttccagt aacgattatt taggtttaag
    28861 ccatcatccg caaattatcc gtgcctggca gcagggtgcg gagcaatttg gcgtcggtag
    28921 cggcggttcc ggtcacgtca gcggttatag cgttgcgcat caggcgctgg aagaagagct
    28981 ggccgagtgg ctgggctatt cgcgggcact gctatttatc tctggttttg ccgctaatca
    29041 ggcggtcatt gccgcgatga tggcaaaaga ggaccgcatt gtcgctgacc ggcttagcca
    29101 tgcctcattg ctggaagctg ccagtttaag cccgtcgcag cttcgccgtt ttgctcataa
    29161 cgatgtcact catttggcgc gattgcttgc ttccccctgt ccggggcagc aactggtggt
    29221 gacagaaggc gtgttcagca tggacggcga tagtgcacca ctggcggaaa tccagcaggt
    29281 aacgcaacag cacaatggct ggttgatggt cgatgatgcc cacggcacgg gcgttatcgg
    29341 ggagcagggg cgcggcagct gctggctgca aaaggtaaaa ccagaattgc tggtagtgac
    29401 ttttggcaaa ggatttggcg tcagtggggc tgcggtgctt tgctcaagta cgatggcgga
    29461 ttatctgctg caattcgccc gccaccttat ctacagcacc agtatgccgc ccgctcaggc
    29521 gcaggcatta cgtgcgtcac tggcggtcat tcgccgtgat gagggggatg cacggcgcga
    29581 aaaactggtg tcactcattg cgcgttttcg tgccggagta caggatttgc cgtttacgct
    29641 ggctgattca tgcagcgcca tccagccatt gatcgtcggt gataacagcc gtgcgttaca
    29701 actggcagaa aaactgcgcc agcaaggctg ctgggtcacg gcgattcgcc cgccaaccgt
    29761 acccgctggt actgcgcgac tgcgcttaac gctaaccgca gctcatgaaa tgcaggatat
    29821 cgaccgtctg ctggaggtgc tgcatggcaa cggttaataa acaagccatt gcagcggcat
    29881 ttggtcgggc agccgcactc tatgagcaac acgcagatct acagcgccag agtgctgacg
    29941 tcttactggc aatgctccca cagcgtaaat acacccacgt actggacgcg ggttgtggac
    30001 ctggctggat gagccgccac tggcgggaac gtcacgcgca ggtgacggcc ttagatctct
    30061 cgccgccaat gcttgttcag gcacgccaga aggatgccgc agaccattat ctggcgggag
    30121 atatcgaatc cctgccgtta gcgactgcga cgttcgatct tgcatggagc aatctcgcag
    30181 tgcagtggtg cggtaattta tccacggcac tccgcgagct gtatcgggtg gtgcgccccg
    30241 gaggcgtggt ggcgtttacc acgctggtgc agggatcgtt acccgaactg catcaggcgt
    30301 ggcaggcggt ggacgagcgt ccgcatgcta atcgcttttt accgccagat gaaatcgaac
    30361 agtcgctgaa cggcgtgcat tatcaacatc atattcagcc catcacacta tggtttgatg
    30421 atgcgctcag tgccatgcgt tcgctgaaag gcatcggtgc tacgcatctt catgaagggc
    30481 gcgacccgcg aatattaacg cgttcgcaat tgcagcgatt gcaactggcc tggccgcaac
    30541 aacaggggcg atatcctctg acgtatcatc tttttttagg agtgattgct cgtgagtaaa
    30601 cgttattttg tcaccggaac ggataccgaa gtggggaaaa ccgtcgccag ttgtgcactt
    30661 ttacaagccg cgaaggcagt aggttatcgg acggcaggtt ataaaccggt cgcctctggc
    30721 agcgaaaaga ccccggaagg cttacgtaat agtgacgcgc tggcgttaca gcacaacagc
    30781 agcctgcagc tggattacgc aacggtaaat ccttacacct tcgcagaacc cacctcgccg
    30841 cacatcatca gcgcgcaaga gggcaggccg atagaatcat tggtaatgag cgccggatta
    30901 cgcgcgcttg aacaacaggc tgactgggtg ttagtggaag gtgctggcgg ctggtttacg
    30961 ccgctttctg acactttcac ttttgcagat tgggtaacac aggaacaact gccggtgata
    31021 ctggtagttg gtgtgaaact cggctgtatt aatcacgcga tgttgactgc gcaggcaata
    31081 caacacgccg gactgactct ggcgggttgg gtggcgaacg atgttacgcc tccgggaaaa
    31141 cgtcacgctg aatatatgac cacgctcacc cgcatgattc ccgcgccgct gctgggagag
    31201 atcccctggc ttgcagaaaa tccagaaaat gcggcaaccg gaaagtacat aaaccttgcc
    31261 ttgttgtagc tatcctgtat ttggttaaat tgcgagcg
//
LOCUS       DAPPNJ010000035        30046 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000035 DAPPNJ010000000
VERSION     DAPPNJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.035"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="RPM73_001268"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="RPM73_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDX6519645.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="RPM73_001269"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="RPM73_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDX6519646.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="RPM73_001270"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="RPM73_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDX6519647.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="RPM73_001271"
     CDS             complement(1087..1581)
                     /locus_tag="RPM73_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDX6519648.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="RPM73_001272"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="RPM73_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDX6519649.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="RPM73_001273"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="RPM73_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDX6519650.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="RPM73_001274"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="RPM73_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDX6519651.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="RPM73_001275"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="RPM73_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDX6519652.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="RPM73_001276"
     CDS             complement(6745..7032)
                     /locus_tag="RPM73_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDX6519653.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="RPM73_001277"
     CDS             complement(7151..8038)
                     /locus_tag="RPM73_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDX6519654.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="RPM73_001278"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="RPM73_001278"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDX6519655.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="RPM73_001279"
     CDS             complement(9275..9769)
                     /locus_tag="RPM73_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDX6519656.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="RPM73_001280"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="RPM73_001280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDX6519657.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="RPM73_001281"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="RPM73_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDX6519658.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="RPM73_001282"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="RPM73_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDX6519659.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="RPM73_001283"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="RPM73_001283"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDX6519660.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="RPM73_001284"
     CDS             13861..14520
                     /locus_tag="RPM73_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6519661.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="RPM73_001285"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="RPM73_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDX6519662.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGVRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="RPM73_001286"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="RPM73_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDX6519663.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="RPM73_001287"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="RPM73_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDX6519664.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="RPM73_001288"
     CDS             17948..18874
                     /locus_tag="RPM73_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDX6519665.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKAANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="RPM73_001289"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="RPM73_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDX6519666.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="RPM73_001290"
     CDS             complement(19225..21444)
                     /locus_tag="RPM73_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDX6519667.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTVIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="RPM73_001291"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="RPM73_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDX6519668.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="RPM73_001292"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="RPM73_001292"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDX6519669.1"
                     /translation="MLYIFRLIITLIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24013..26271)
                     /gene="parC"
                     /locus_tag="RPM73_001293"
     CDS             complement(24013..26271)
                     /gene="parC"
                     /locus_tag="RPM73_001293"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDX6519670.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26817..27299)
                     /locus_tag="RPM73_001294"
     CDS             complement(26817..27299)
                     /locus_tag="RPM73_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6519671.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27352..27744)
                     /gene="ygiW"
                     /locus_tag="RPM73_001295"
     CDS             complement(27352..27744)
                     /gene="ygiW"
                     /locus_tag="RPM73_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDX6519672.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27896..28555
                     /gene="qseB"
                     /locus_tag="RPM73_001296"
     CDS             27896..28555
                     /gene="qseB"
                     /locus_tag="RPM73_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDX6519673.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28552..29901
                     /gene="qseC"
                     /locus_tag="RPM73_001297"
     CDS             28552..29901
                     /gene="qseC"
                     /locus_tag="RPM73_001297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDX6519674.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29947..>30046)
                     /locus_tag="RPM73_001298"
     CDS             complement(29947..>30046)
                     /locus_tag="RPM73_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HDX6519675.1"
                     /translation="REMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7377 a         8060 c         7610 g         6999 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagagcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcact ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg ccccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacaa
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgtta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccagc aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caattggtgt ttttttaagc aaagcataag cgcgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctaa acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acactaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtaaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattaaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa ctggttaagc
    14341 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gcttgtgaca gacttaaaat tatggtggaa cgccgatacg ctcatattgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcacccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctt aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gccgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggtcaat gccttctata aaaccacctt caaggcggcc aacaatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatat
    18721 tcgttttacg ctgactaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgc ttgtcgccct tcggtacgaa
    19561 gacgtcttca ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcacggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag cagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcaag gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacagcgcc
    24001 ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc
    24061 aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt
    24121 gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat gaatggtcag
    24181 cgtgctttgc ggcggcagaa cgtacaattg cgccagacca tcttctccac gcgcggcttc
    24241 tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct
    24301 taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc
    24361 ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca aagccttacc
    24421 tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga
    24481 agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg
    24541 cggcaacgtt aatttgccgg tgagcggctc gccctgacca cgcgccgacg gcagcgtaat
    24601 cgggtcaatg gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt
    24661 acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat
    24721 atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc
    24781 agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga
    24841 acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt
    24901 cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact
    24961 ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag
    25021 gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc
    25081 atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc
    25141 ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac
    25201 ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg
    25261 acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt
    25321 agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac
    25381 cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt
    25441 tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg
    25501 taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga
    25561 accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa tttccgcttc
    25621 agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg
    25681 gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt
    25741 cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgagcagg
    25801 cagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg
    25861 ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat
    25921 tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg
    25981 ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg cgctatcgcc
    26041 gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc
    26101 gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttcag
    26161 accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta
    26221 ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga
    26281 ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct
    26341 gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga
    26401 taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg
    26461 gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc
    26521 gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag
    26581 tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag
    26641 tgcggcttgc tcatggatac ccccgagacg gcttacggcg caccggataa taaatttcgc
    26701 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    26761 tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac
    26821 gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg
    26881 gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg
    26941 cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg
    27001 gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt
    27061 tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg
    27121 tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc
    27181 cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac
    27241 atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt
    27301 ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt
    27361 tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc
    27421 ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtggtcgat
    27481 atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc
    27541 aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac
    27601 ggtcgttacg ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg
    27661 gccagaaaaa ccgccctgct ctgctgccat caccggtgcg ctgcacaggg ccattactgc
    27721 gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt
    27781 taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttagt gctgtacaga
    27841 gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg
    27901 aattttactg atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa
    27961 aatgggtttt agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag
    28021 cgcgccttat gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat
    28081 tttgcgcgaa tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga
    28141 tgcgctggag gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc
    28201 ttttgcgttg atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca
    28261 ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac
    28321 gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat
    28381 gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga
    28441 cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg
    28501 tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt
    28561 acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg
    28621 acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg
    28681 ttcgacaccc aactgatgct gtttgccaag cggttaagta cgctcgatct caacgaaatc
    28741 aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac
    28801 gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat
    28861 aacggagaag atattcctta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtg
    28921 ggcgataaag atcaatggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc
    28981 gttgttggtc aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa
    29041 ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt actattgggt
    29101 cgtgaactcg cgccgctgaa caaactggcg ctggcgctgc gtatgcgtga ccctgactcg
    29161 gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc gtccgttggt tgagtcgcta
    29221 aatcaactgt tcgcccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac
    29281 gcagctcacg aactacgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag
    29341 ctttctgacg atgatccgca agcgcggaaa aaagcgctgc ttcaattaca ttacgggatc
    29401 gatcgcgcta ctcgtctggt tgatcaactg ctcactctat cgcggctgga ctcactggat
    29461 aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat
    29521 atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc
    29581 atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac
    29641 gccgtgcgct acagtccaca gggcagcgtg atagacgtca cgctgaatgc tgataatttc
    29701 atcgtgaggg ataacggccc cggtgtgacg ccagaggctc tggcgcgaat tggcgaacgc
    29761 ttctatcgcc cccccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag
    29821 cgaatcgcca aattgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt
    29881 gaggcgaagg taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag
    29941 attttactaa tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca
    30001 aataccgagc gaaaatacat atacccaaaa ctcgaacatt tcccgc
//
LOCUS       DAPPNJ010000036        30016 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000036 DAPPNJ010000000
VERSION     DAPPNJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.036"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1586
                     /gene="rtcR"
                     /locus_tag="RPM73_001299"
     CDS             <1..1586
                     /gene="rtcR"
                     /locus_tag="RPM73_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDX6519676.1"
                     /translation="AFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHD
                     TRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQPEKED
                     YLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRYN
                     AIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSF
                     LARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSA
                     NGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVA
                     EGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTEARRAW
                     LAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQESRPSAL
                     TALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDADRLRK
                     YLARFGLTWEAVQDQHSSS"
     gene            complement(1568..2326)
                     /locus_tag="RPM73_001300"
     CDS             complement(1568..2326)
                     /locus_tag="RPM73_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDX6519677.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2343..3173)
                     /gene="glpG"
                     /locus_tag="RPM73_001301"
     CDS             complement(2343..3173)
                     /gene="glpG"
                     /locus_tag="RPM73_001301"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDX6519678.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3218..3544)
                     /gene="glpE"
                     /locus_tag="RPM73_001302"
     CDS             complement(3218..3544)
                     /gene="glpE"
                     /locus_tag="RPM73_001302"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDX6519679.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3734..5239
                     /gene="glpD"
                     /locus_tag="RPM73_001303"
     CDS             3734..5239
                     /gene="glpD"
                     /locus_tag="RPM73_001303"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDX6519680.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5293..5898)
                     /locus_tag="RPM73_001304"
     CDS             complement(5293..5898)
                     /locus_tag="RPM73_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519681.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5901..6668)
                     /locus_tag="RPM73_001305"
     CDS             complement(5901..6668)
                     /locus_tag="RPM73_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519682.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(6671..7381)
                     /locus_tag="RPM73_001306"
     CDS             complement(6671..7381)
                     /locus_tag="RPM73_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519683.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(7396..8904)
                     /locus_tag="RPM73_001307"
     CDS             complement(7396..8904)
                     /locus_tag="RPM73_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDX6519684.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(9030..11477)
                     /gene="glgP"
                     /locus_tag="RPM73_001308"
     CDS             complement(9030..11477)
                     /gene="glgP"
                     /locus_tag="RPM73_001308"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDX6519685.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11496..12929)
                     /gene="glgA"
                     /locus_tag="RPM73_001309"
     CDS             complement(11496..12929)
                     /gene="glgA"
                     /locus_tag="RPM73_001309"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDX6519686.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12929..14224)
                     /gene="glgC"
                     /locus_tag="RPM73_001310"
     CDS             complement(12929..14224)
                     /gene="glgC"
                     /locus_tag="RPM73_001310"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDX6519687.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14242..16215)
                     /gene="glgX"
                     /locus_tag="RPM73_001311"
     CDS             complement(14242..16215)
                     /gene="glgX"
                     /locus_tag="RPM73_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDX6519688.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16212..18398)
                     /gene="glgB"
                     /locus_tag="RPM73_001312"
     CDS             complement(16212..18398)
                     /gene="glgB"
                     /locus_tag="RPM73_001312"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDX6519689.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18671..19774)
                     /gene="asd"
                     /locus_tag="RPM73_001313"
     CDS             complement(18671..19774)
                     /gene="asd"
                     /locus_tag="RPM73_001313"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDX6519690.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19967..20560
                     /gene="yhgN"
                     /locus_tag="RPM73_001314"
     CDS             19967..20560
                     /gene="yhgN"
                     /locus_tag="RPM73_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDX6519691.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20606..21901)
                     /locus_tag="RPM73_001315"
     CDS             complement(20606..21901)
                     /locus_tag="RPM73_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDX6519692.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDAEYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21808..23904)
                     /locus_tag="RPM73_001316"
     CDS             complement(21808..23904)
                     /locus_tag="RPM73_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDX6519693.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(24044..25384)
                     /gene="gntU"
                     /locus_tag="RPM73_001317"
     CDS             complement(24044..25384)
                     /gene="gntU"
                     /locus_tag="RPM73_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDX6519694.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25388..25915)
                     /gene="gntK"
                     /locus_tag="RPM73_001318"
     CDS             complement(25388..25915)
                     /gene="gntK"
                     /locus_tag="RPM73_001318"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDX6519695.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(26054..27049)
                     /gene="gntR"
                     /locus_tag="RPM73_001319"
     CDS             complement(26054..27049)
                     /gene="gntR"
                     /locus_tag="RPM73_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDX6519696.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27273..27968)
                     /gene="yhhW"
                     /locus_tag="RPM73_001320"
     CDS             complement(27273..27968)
                     /gene="yhhW"
                     /locus_tag="RPM73_001320"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDX6519697.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(28091..29128)
                     /locus_tag="RPM73_001321"
     CDS             complement(28091..29128)
                     /locus_tag="RPM73_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX6519698.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            29461..29949
                     /gene="yhhY"
                     /locus_tag="RPM73_001322"
     CDS             29461..29949
                     /gene="yhhY"
                     /locus_tag="RPM73_001322"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX6519699.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7501 a         8056 c         7400 g         7059 t
ORIGIN      
        1 tggcttttgg ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt
       61 cgaaatggcg tccgacgctt tgtttatgcc agcaagaatc gttagtcatc gatcggctgg
      121 aattgttgca cgacacccgc tcgcgctcgc tattcgaaac gcttaaacgc gatatcgcca
      181 gcgtttcgcc agaaacagaa gtcgtgggcg ttgagattga actgcataac ccgtgggatt
      241 tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa
      301 aagaagacta tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc
      361 tgctggcgga agcgcgttac ctgcccgccc ggctgataca atcttcacct ccgcgcaaaa
      421 aagaacagcc gcgcggcgca ggtgaagtga cgattatcga tctcgattta agccgttata
      481 acgccatcgc cagccgcttt gccgaggaac gccagcaaac gcttgatttt cttaagtccg
      541 gcattgccac gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga
      601 tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg cgcgggcaag tcatttctgg
      661 cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt tagcggcgcg tttgtggaag
      721 tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac gctgtttggt catgtaaaag
      781 gcgcgtttac cggggcgcgg gaatctcgtg aaggtttatt acgcagcgcc aacggcggaa
      841 tgttgtttct tgatgagatt ggcgaactgg gcgcagacga acaagcaatg ctgctaaaag
      901 ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc
      961 agcttatcgc cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaatttcgcg
     1021 aagatctgta cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc
     1081 aggaagatat tgaaccgaac cttgattatg aagtggagcg ccacgccaca ctcactggcg
     1141 acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt ggcttttgcg acctctcccc
     1201 aggcaacatg gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct
     1261 ttgccactag cggacgcatc actctggacg tggttgaaga tgagataaac cgtctgcgct
     1321 ataactggca ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa
     1381 acatcgacct ttttgacagg ctgcaactgg aacacgtgat agctctctgt cgccaggcaa
     1441 agtcgctttc cgctgccgga cgcctgcttt ttgatgtttc gcgccagggc aaagccagcg
     1501 tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg
     1561 tgcaggatca gcacagctcc agttgaatat gatggtccgt cagcacctgc attacgctta
     1621 ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct gatgctgccc atattgacca
     1681 tcgcgttacg gccaaatttc gagtgatcga caaccagcat aacgtggcgc gagttctcaa
     1741 taatggcgcg cttggtgcga acttcgtggt aatcgaactc cagcagcgag ccgtcgctat
     1801 cgatgccgct tatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg
     1861 tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc gccaccggcg agaataatgc
     1921 ggaaatcttc ttttaccatc aacgtgttag caacattgag attgttggtg acaatacgca
     1981 aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa
     2041 acagtgtcga gccattgggg atttgctccg ccactttgcg ggcaatcctc tctttttctt
     2101 cggtctgggt ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg
     2161 caccgccatg gtgacgcagg atcaggtttt gctccgccag ctcattgagg tcgcggcgaa
     2221 tagtctgcgg gctgacggag aaatgctcta ccagctcttc agtactgaca taaccctgct
     2281 gtttaaccag ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg
     2341 aattattttc gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct
     2401 aacccggcga tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg
     2461 acaatccaga tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga
     2521 tcgcgttcgc cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg
     2581 ccaaaccacg gcccgctgaa tttttgctgc acatagccgc ttaacagggc gctaatgaga
     2641 gtaataacaa ttagcttacc gctaccgagg cgtttttcca ccgcaccgcc gagataccac
     2701 caccagagca ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag
     2761 taacgccaga actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact
     2821 tcctgatcgc cgagaatttg catggcgata aacaccacca cgcaggcgat catcaccacc
     2881 caggttaccg gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc
     2941 agaccactgc cggtatggcc cgcctgccag ctggccgcca gataacgcgg atctgccggg
     3001 ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc
     3061 cagacatcgc tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatataa
     3121 tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct
     3181 gtcgcttatt ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg
     3241 cgggaaactg acgctgccag gcttcaaaac cgccgtcaat gctatagacc acatcgtagc
     3301 cctgttgcag cagatactgc gccgcgcctt tgctgctatt gccgtgataa cacatcacca
     3361 tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat
     3421 ggaaagcctg caccgcatgc cccattgcga aactctgtgg atcgcgaata tcgaccagca
     3481 ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat
     3541 ccatgcgtct ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa
     3601 gcggcagata aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc
     3661 taaagcgcga ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt
     3721 gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc
     3781 tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga
     3841 tctcgcttgc gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct
     3901 tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa
     3961 aatggctccg catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg
     4021 cccggcgtgg atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag
     4081 cctgccggga tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa
     4141 gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc
     4201 ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg
     4261 ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag
     4321 ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga
     4381 ggggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt
     4441 gccgcgtgtg catacccaga agcaggccta cattctgcaa aacgaagata aacgtattgt
     4501 gttcgtgatc ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa
     4561 aggtgatccg aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta
     4621 taacacgcac tttaaaaagc agttaagccg tgacgatatc gtctggacct actccggtgt
     4681 gcgtccgctg tgtgatgatg agtccgactc gccgcaggct attacccgtg attacaccct
     4741 tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagttgac
     4801 cacctaccgc aaactggcgg aacatgcgct ggaaaaactg acgccgtatt atcagggcat
     4861 tggcccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga
     4921 cgattatgcc gctcgcctgc gccgccgcta tccgttcctg actgaatcgc tggcgcgtca
     4981 ttacgctcgc acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag
     5041 cgatctcggg gaagatttcg gtcatgagtt ctacgaagcg gagctgaaat acctggtgga
     5101 tcacgaatgg gtccgccgcg ccgacgacgc cctgtggcgt cgcacaaaac aaggcatgtg
     5161 gctaaatgcg gatcaacaat ctcgtgtgag tcagtggctg gtggagtata cgcagcagaa
     5221 gttgtcgctg gcgtcgtaag gatgatgccc ggttgtgaag tgcaaccggg ctatttggat
     5281 tccttagact attcattgta aataattgaa taacggcccc tgagctaaaa tcgccaaact
     5341 taagcacgtt acccaatcgc cccacttctt cctctggttt ctattttttc tgcaccaatg
     5401 gcaataatat cccaatgcaa aaccaagctg tgcactaatc gggccgacaa aatcagtcca
     5461 aggctgcggc caaaaagggg aaaaattcag gggaagccag acaaacgtca gaaagaaatt
     5521 tcggatattt tccagcaacg ctgatttctc tggagaaata taaagaatga aattcgagag
     5581 aattaaccag aaatagaccg gcgaaaagac cagccagcaa tgcagtctgt tttttaattc
     5641 aggaggcgtt ctgcgggaga attgccaacc gatgccagcc attacactga aaaaaagtgc
     5701 atattcaacc attaactcaa gttgtagttc ctgaagagaa atacgcacaa ccaggagcgg
     5761 aaaggagaat attatttctg ccagaaaagt taacgtgaat ataagtacgg ctaagacaat
     5821 aaccgtcggc cataccaata atggcacgat aacaaaaccg agaattacag ggggaatata
     5881 aagccggaat ttaatcattt ttattttcct ggtgattcgg atgatgcgtc atacatcgcg
     5941 cataaacact catcaattat ccagtcgtct acccagacct gatgctgtgt gccctgttct
     6001 ggctgctttg gtgggttctc aatcgcctgc cgcgcgcgtt ctttcaagat ggattgattg
     6061 ttgaaggccc aggcattgat ttgggtggta atgacgttat cgatatcgtc ctggttatct
     6121 ctgatataat cctcacaata tctgttcaat aaacaggcaa gagaaacggt cgaccaaaag
     6181 ccgggaactg aattaatggc atccagcaat ggcgcaagag cgatatcggc acggaaaaac
     6241 atttccagca tatactgtaa ttcgacagct tcatcgccgg atacccaatc agtcatatac
     6301 tggcaggcaa atcgttgcaa aattttccat tcatcctcac gccaattttc tttactggca
     6361 aggtttatac gccgtagaga aaatacttcg tgaatattgt caaactgccc ctgtgcaaca
     6421 aattccagta aacgcggcag aaaatagcgg acctcatcgc tattttgcgg atcaggccct
     6481 gggctactat tccatgcatt gattaaggaa tacgagatcg ctcgtaacga tgtactccgt
     6541 aatacctttt gttcctgctg actcaggcaa tattcacaac aaaccacaaa tttctgcggc
     6601 accggataac gacgaaaaat atgataaatc tgctcaatta atgcctgcat ggcggggctg
     6661 ggttgcataa ttaccccctc gcccgctgat cgcgtgtaaa cggttgataa acaaaacgag
     6721 cattcgccac gttcaggcga ataatggcac tacgagagag ggaaaagctg gggcgaatgc
     6781 aactattgca gtctcgacag ctttcttcag cgcaattttc cactgtgtaa taacgagcgt
     6841 agagtttcag ttccgcttgc ttatcacgaa atccgcgtaa actgttatcg ttatgaaatt
     6901 tccaggcatc tttaaagacc tgaaaaatgt agatatcctc aatccaggac tcacattttt
     6961 taagcgggcg ctttgctatg atcactcgct catccgggtt tagcatcgcc gtaaattgaa
     7021 cggtgaggtc atttgtcgtg ttcagatcac attgaacatc tggtacatca ctgatatagg
     7081 ggtcaaatcc aaagtcagca ccacaggcac aaagacggct aatggtggcg gtatcgaggg
     7141 aaaatcccgg ccatacattg ctggacgtta agccaatatt taatgtgaca ttatatcgaa
     7201 gcaatccctc aagaccgtca gcaccgacga acttttcccc ttcatccagg gcctggttaa
     7261 tgagttgctg aacaatgcct tcatcccagt atgccgattt gcagttggca atcgtaatat
     7321 ccacgcctgt gaatggatca gtaccgacaa ggcttgcaaa taatgtatta actaattcca
     7381 tttttattat ccgccttaat gcaacgtttt atacatcggc acataaccta catcttccac
     7441 caggctctgc ccctgcggcg ttaaaaacca ctccaccaat ttttgtgttt ctgacgtcat
     7501 attttcccgc gttaccataa aggcatcgac gatataggga tatttgccgt tgcgaatgtt
     7561 ttcggcagtc ggtgcaatac cgttaatcgc tagcagtttt atatttttat cggcgttcat
     7621 ttgtgttgcg taataacgga aggtgtaacc tattgcgttg tttgtattac ggtattcggc
     7681 aacgacttta atcattccct ccatcatact tgccacttct gtttcctgcg gcgagatcat
     7741 gcggacattt ttcatgacct gggattgcat caccgtctgg ctaccagagt cttccgggcg
     7801 ttgccaggtc tggatctcct gatcgttacc gccaacagta cgccagttgg taattgcacc
     7861 actgaagatg tcacgcacct gttgttcagt cagggaatta accgggttat ccgcattgac
     7921 gatgaaaaca aacgcttcgc gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc
     7981 agcgcgtttt ttctgcccac cggaaggttg cgccacaaaa ataatatcag catcaccttt
     8041 aacaatcctg ttatacgcat cgggggtacg agagctctcc agatattccc tggtgtgaaa
     8101 atcttccggt ataacgctta aagcataaaa tgcagaggca taaattgggt acgccgccgt
     8161 agcaccatcg agtcgcggcc agttttgcgt aaactgaatt tgcggtttac cacgcagtgg
     8221 tgtcagttga ttattgagtt tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac
     8281 accgacgccg ggattgagct gtttatataa ataaccctga taaccagcga caaaaccaag
     8341 catgagaagg ataaacagga gggaattacg gcaacgtagc ccggatgtac cggttaaagg
     8401 atgtctggtt ggccggccgt accccagcga aaaactgata tgcgcgccaa cgggtataat
     8461 gactatccac cagtactcac ctccgactac tgagactaac aaaacgccca gccaggggag
     8521 cataagatca gccaaccaac ccgatcccat ctggtgaaat tgataatctg ccagatgcag
     8581 cgcgattatc cagccaacca ggattaataa accgggtact aaaaaaagac attgtcgcca
     8641 gacggctatt tctctttttt ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa
     8701 tagcattaat gcgcccagcg ccagcataaa aatgttgagc agaaggtaac ttgttggcgt
     8761 gataaggata ttacaaactt caataatacc ggcaagtccg acacccagca tagcaacaac
     8821 cgccgccaaa aattgcgtca gtatggggat gccgaaaaaa gtcattacca gcgaggacaa
     8881 aatccatttt ctgttttgca ttattctttc cattcttttt gaatggtgaa attatactcc
     8941 ccgagtcccc ttgccccttc tggacacttt tccgaaatga tggcgaaaaa aaacgggacc
     9001 ctttggcccc gttctattta ttagtgaaat tacaatctca ctggatcaat atgccagata
     9061 tgatcggcgt actctttgat cgtacggtca gaagagaagt agcccatatt ggcaatgttc
     9121 agcatcgctt ttgcggtcca ctcttcctga agctcgtaga gttcgtcgac tttatcctga
     9181 caatcgacat agctgcgata atccgccaga acctgatagt gatcgccgaa gttgatcagc
     9241 gaatcaacca gatcgcgata gcgacccgga tcttccggac tgaatacacc gctgccgatt
     9301 tgcgtcagca cctgatgcag ctcctcatct ttctcgtagt attcacgcgg tttgtagccc
     9361 tgacgacgca gttcttccac ttcttccgca gtgttaccaa agataaagat attgtcagca
     9421 ccgacatgat ccagcatctc gacattcgca ccgtccagcg taccgatagt cagcgcgccg
     9481 ttaagcgcaa acttcatgtt actggtgccg gaagcttccg tccccgccag cgaaatctgt
     9541 tcagacagat cggccgccgg aatgatcaac tgcgctaggc taacgctgta gttcgggatg
     9601 aacacgactt tcagcttgtc gccaatctgc ggatcgttgt tgatcacttt cgctacgtca
     9661 ttgatcaaat gaataatgtg cttcgccatg taataggccg aagccgcctt accgccaaaa
     9721 atattcacgc gcggtaccca cttcgcatcc ggatcggcct tgatgcggtt atagcgggta
     9781 atcacatgca acacattcat caattgacgt ttgtattcgt gaatacgttt gatttgcaca
     9841 tcgaacaacg cctttggatt caccaccaca ttcagctgct gggcgatata ctccgccaga
     9901 cgctttttgt tctccagctt cgcctgatgc acagcgtgat taaccattgg gaaatcacag
     9961 tgttgttgca gctcattaag caggctaagg tcggtgcgcc agttacggcc caggtgttcg
    10021 tccagcacgg ctgaaagcga tgggttcgct accgctagcc agcgacgcgg cgtcacaccg
    10081 ttggtgacgt tggtgaaacg acccgggaag attttcgcaa agtcggcaaa caacgattgc
    10141 accatcagat tagagtgcag ttccgataca ccgttaactt tgtggctcac aacaaccgcc
    10201 agccaggcca tacgcacacg acgaccgttg gattcatcaa tgatcgacgc ccgtcccagc
    10261 agatcggtat cgttcggata ctgttcctgc aaggttttca ggaaatagtc gttgatttca
    10321 aagatgatct gcaggtgacg cggcagaatt ttacccagca tatcaaccgg ccaggtttcc
    10381 agcgcctcgc tcatcagcgt gtggttagtg taggagaaga cctgacaaca cacctcaaac
    10441 gcgtcgtccc agctaaattg gtgctcatcg atcagcagac gcatcatctc aggaatcgac
    10501 agtaccggat gggtatcatt gagatgaatc gcgattttat ccgccaggtt atcgtaggtt
    10561 ttatgcaact gataatggcg gcttaaaatg tcctgaatgg tcgaggaaac caggaagtat
    10621 tcctgacgca ggcgcagctc acgcccggag taggtggagt catccggata cagtacgcga
    10681 gatacgttct cggagtggtt tttatcttcc actgccgcga agtagtcacc ctggttgaat
    10741 ttaccgaggt taatttcgct actggcttgc gcactccaca aacgcaacgt gttggtcgcg
    10801 tcggtgtcgt aaccagggat tatctgatcg taagcgactc ccagaatctc ttcggtttca
    10861 atccagcgcg tttttttacc ttcctgctga atgcgaccac caaaacggac tttatagcgc
    10921 gtgttgtggc gtttgaattc ccacgggtta ccgtattcca gccagtagtc tggcgactct
    10981 ttctggctac cgttaacgat gttctgcttg aacataccgt agtcatagcg gatgccgtaa
    11041 ccgcgccccg gcaaccctaa cgtcgccaga gaatcaagga agcaagccgc cagacgtccc
    11101 aggccaccgt taccgaggcc tgggtcattt tcttcatcaa tcagctcttc gagatttaac
    11161 cccatcgctt ccagtgcgcc ctgtacatct tcgtaaattc ctagcgacaa catggcgttg
    11221 gagagcgtac ggccaatcaa aaactccatc gacaggtagt aaacctgacg agtttcttgc
    11281 gacaactggg cacggtttga acgtaaccag cgctccacga gacgatcgcg cacagcaaat
    11341 aacgttgcgt tcagccattc atgtttattg gcgacgaccg ggtcctttcc aatcgtaaac
    11401 atcagcttgt aagcgataga gtgcttaaga gcttctacgc taagcgtggg cgatgaatat
    11461 gtaaacggag cattcatata ggcgtttcct gaaaactatt tcaagcgata gtaaagctca
    11521 cggtacgact tcgccgcgac ctgccagcta aaatccattg ccatagcctg acgttgcaca
    11581 aaccgccaca gtgaaggacg ggaccacagt acaaaagcac gccgaatagc ccgtaacagc
    11641 gaccaggcat tactatcttc aaagacaaac ccactggcga cgccatctgc aaggttctca
    11701 agagaacagt cagaaaccgt atcagcaagc ccaccggtgc gccgcactaa cggcagcgta
    11761 ccgtacttca atccataaag ttgcgttaag ccgcacggtt caaaacggct gggcaccaga
    11821 atgacgtccg cgccgcccat aatgcgatgc gaaaatgctt cgtgatagcc aatctgaacg
    11881 cccacctgac cggggtattc cgctgccgcc gcaaggaaac cttcctgcag caccggatcg
    11941 cccgcgccga gtagcgccag ctgcccgccc tgctccagaa ggcccggtaa ggcttccagc
    12001 accaggtcga gacctttctg gctggtcaga cggctcacca ctgcaaaaag cggcacttta
    12061 tcatcaacct taagccccat tgcgatttgt aactggcgct tattttccgc tttatcttcc
    12121 aacgtatcgc gggtgtagcg cgaggccaac agtaagtccg tctctggact ccagattttc
    12181 tcgtctacgc cgttcagtac gccggaaaga cgcccttcac ggtgacgctg ttgcaacaga
    12241 ccttccatac cgtaggcaaa ctgcggttcg gtgatctcgc gagcgtaggt tggactgacc
    12301 gccgtaatgt gatcggcata gtacagaccg gccttcagga aagagatttg tccgttgaat
    12361 tccagcccat gaatattaaa gaatgaccat ggcaattgga tgtcattcat atgatgtgca
    12421 taaaacatgc cttgataggc caggttgtgc acagtaaaca ccgacttcgc cggacgcccg
    12481 cgcgccgcca gatacgcagg cgcaaggcct gcatgccagt cgtgcgcatg caccacatca
    12541 ggacgccaga atgggtcaag cccgctggcc atttctgccc caacccaccc cagcagcgca
    12601 aaacgcaata cgttgtcggt ataggcaaat aagttggtat cgtgatacgg gcttcccgga
    12661 cgatcataga gatgcggcgc gtcaatcagg tatatgccaa ccccgttgta atgaccgaac
    12721 aacagcgtga tatgtccggc gaaggtatca cgacgggata ctacctgcgc atcggtaacg
    12781 ccacggcgaa tatcgggaaa tgcaggcaac agtacgcgag cgtcaacgcc gtctgcgatt
    12841 tgtgctgcgg gtaatgcccc aataacatca gccagaccgc cggttttaag cagcgggaac
    12901 atctctgaac atacatgtaa aacctgcatt atcgctcctg tttatgccct aacttccgta
    12961 gcatttcgcg cgttaccagc acgatgcctt cttctgaacg atagaaacga cgtgcatctt
    13021 cctctgcgtt ttcaccaatc accatgcctt ccggaataac acaagcacga tcgatgacgc
    13081 agcggcgcag acggcacgag cgacctaccc atacttccgg taacaatacg gcggaatcaa
    13141 tgttgcagaa tgaattcacg cgaacgcgcg agaacagaac ggactgcacc accaccgaac
    13201 cggagatcac acagccgccg gataccagtg agttaagggt catcccgtgg ctaccggagc
    13261 gatcctgcac gaatttcgct ggcggtaatg attcattgta ggtgcgaatt ggccaattgc
    13321 gatcgtacat atccagttcc ggcaccacag aggccagatc gaggttcgct ttccagtaag
    13381 cttccagcgt acccacatcg cgccagtacg gctcggcatc cgggtcggat tgtacgcaag
    13441 agagcgggaa cgggtgcgca taggccagac cggcttcggt gatcttggga atcaaatctt
    13501 tgccaaagtc gtggctggag ttctcatcac gatcgtcttc ttccagcagt tcatacagat
    13561 agtcggcgtc aaagacgtag atacccatac tcgccagaga tttgctcgga tcgttcggca
    13621 ttgacggtgg gttagcaggt ttttcaacga attcgataat tttatcgttc tcatcaaccg
    13681 ccataacgcc aaatgcggag gcttcttcaa tcggtactgg catacaagca acggtgcaac
    13741 gtgcgccttt ttcgacgtga tcgataagca tacgcgagta gtcttgcttg tagatatggt
    13801 cgcccgccag gatcaccacg tattccgctt tataacggcg gataatgtcg aggttttggg
    13861 tgaccgcatc tgcggtgccg cgataccagt tttccccttt cattctctgc tgtgctggca
    13921 gcagatcgac aaactcgttc atttcttcat tgaagaatga ccagccgcgc tgaatgtgct
    13981 gcaccagagt gtgggactgg tactgggtga tcacgcccat acgacggatc ccggagttga
    14041 tgcagttaga cagcgcaaag tcgataatgc ggaacttacc gccgaagtgt acggccggtt
    14101 ttgctcgctt attggttaaa tccttcaggc gggtaccacg tcctcccgcc agtatcaggg
    14161 caacagattt caatggcagc tggcgcgcca acattaagtg atcgttcttc tctaaactaa
    14221 ccatgactaa ctcctttttt atcatctctg gaacacacac aatccgtgtg caggtccctg
    14281 ccagacagcc gtaatcactg ggttatcctc tccagcgaat gggggaatgg cgtgccactc
    14341 cccagcaggt aaaacaatct ctgttacctc aagcgtggcg ttaattgcga tcaaaaagcg
    14401 atccgagagc agaatttgca gctgtttcgg cccgttttgc cactcatccg tgcttaaagg
    14461 ttgagcatat cgatttagcc aacggacatt gccgtcgcct tcttcccacc agcgattctc
    14521 caccaaagcg ggaatgcgct tgcgcagatg gattaacgcg gcggtaaatg cggttaaacc
    14581 actgcttgcc tgcgaccagt ccaaccaggt taattggtta tcctgacagt aggcattgtt
    14641 attgccatgc tggctgtgac catgttcgtc accggccagt aacatcggcg taccctggga
    14701 gagcaacaac gttgttaaca gggcgtgaat gctgtcgcgc cgccgttcaa ccaggtcaag
    14761 agaaccgcct aacccttctt taccatgatt gttactgtaa ttgttgttgg tcccgtcgcg
    14821 attttcttct ccgtttgctt cattgtgttt atggttgaag caaacgcagt cgcgaagcgt
    14881 aaaaccgtca tgcgcggtga cgagattaat cgcggcactc ggcagacgac cattacgttt
    14941 aaaaacatcg ctggaggcag caaaacgccc ggcaaacgcc cccagaggca aatcataatg
    15001 tagccagaaa cgacgggcag catcgcggaa atgatcgttc cactcggcaa acagcggcgg
    15061 gaaatttccc acctgataac caccaggagc gatatcccac ggttcagcaa ttaacttcac
    15121 ctgcgagagc accgggcagt tctggatagc ggtaaacaac ggcgcatcct gacggaactc
    15181 tggcgtacgg cccatgactg ccgccagatc aaagcggaaa ccatcgacgt ggcaggtttc
    15241 tacccaataa cgcaggcagg cgctggcata atccaccacc gccggatgac tcaaattgag
    15301 cgtgttgccg caaccggtcc agttgtgata atcgccgtct tctcttatcc aataatagct
    15361 acggttatcg atcccacgca gcgagaataa cgggccgtcg aggtccagtt ccgcactatg
    15421 gttgagcacg atatcaagaa tgacttcgat acccgcttta tgcagtgctt tgattgcatc
    15481 gcgaaactca tccagcgccg tttctggcga gcaggcatac gccggatgca gcgcaaacat
    15541 cgccaccggg ttgtaacccc agtagttact tagccccatg cgttgcagac gtggttcact
    15601 ggcaaactgc gccactggca gcagttccag cgcggtaatg cccaattgtt tcaaatagtt
    15661 gatcatcacc ggatgcccga gggctttata agtgccacgg atctcgaccg ggatctccgg
    15721 gtgcaagtac gttaatcctt tgacatgggc ttcataaatg atggtgctgc cccacggcgt
    15781 gcgcggcggg gcatcatctt cccagtcata gtgatcaacc accactacgc atttcggcgc
    15841 aatggcggcg ttgtcgcgat agtcaggttc attatgaccg gcgtgcagca gcgggttatc
    15901 tttaaactcc ccgtcaattt gccgcgcgca aggatcaatc aacaacttcg ccgggttaaa
    15961 gcgatgcccc tcggcgggtt gccaggggcc atgaacgcga taaccataac gcaaacccgg
    16021 gcgcgcatcc ggcagataac cgtgccaaat gtcgccactg tgccctggca agtcatagcg
    16081 atgttcctgg ccattggcgt caaagacaca cagttctacc cgctcggcat gagcggagaa
    16141 aagtgtgaag ttgacgccct gaccgtcgta atgcgcgccg aggggagcgg gtttgccaat
    16201 ggcgagttgt gtcattctgc ctcccgaacc agccagatag tggccagcgg tggtagcgtc
    16261 aggcttagtg aatgctgacg accgtggctg gcaatctcat cgctgtgtac cgtgccgcca
    16321 ttgcctgcat tactgccgtg atagtgcatg gaatcggtat tgaggatttc acgccatttg
    16381 cccggctggt ttatgccgaa gcgataatca tgacgcggta ccggcgtaaa gttactggca
    16441 acgatgattt cgttaccctc tttatcgcga cgcacaaaga tcagcaccga gcgttctttg
    16501 tcatccacca ccagccattc aaagccgtac gggtcaaaat ccagttcatg cattgcttta
    16561 tggtggcggt aggtgaggtt cagatcgcgc accagacgct ggacaccgtg gtgccagtta
    16621 tcgccgcctt ccaacagatg ccagtcgagg ctggcgtcat ggttccactc gcggccctgg
    16681 gcaaattcgt tccccatgaa cagcagtttc ttgcccggga atgcccacat ccagccatag
    16741 taggcgcgca ggttagcgaa tttctgccat gcgtcgcccg gcatgcggtc gagaatcgat
    16801 tttttaccgt ggaccacttc atcatgcgac aacggcagga cgaagttttc agtgtagttg
    16861 tagagaatcc cgaaggtgag tttatcgtga tgatactgac gataaaccgg gtcgagcttc
    16921 atgtagtcca gggtgtcatg catccagccg aggttccact tgtaccagaa gcccagaccg
    16981 cccatatcct gcggacgaga aacgccaggg aaatcggtag actcctcagc cattgtcacc
    17041 gcaccggaaa cctgctcacc aagaatacgg ttggtattac gcaagaattc aatcgcttca
    17101 agattctcgc gcccgccaaa ttcgttcggg atccactccc cctctttacg gctgtagtcg
    17161 cgataaatca ttgacgccac cgcatcgacg cgcagcgcat caataccaaa acgttcaatc
    17221 cagtaaagcg cgttaccgac gaggaagtta ctgacttcac ggcgaccata gttgtagatc
    17281 agcgtattcc agtcctgatg atagccttcg cgcggatcgc tgtgttcata caagttcgtg
    17341 ccatcaaatt cggcaagcgc aaagtcatcg gtcgggaagt ggcctggcac ccagtcgaga
    17401 atcacgttca gacccgctgc gtgtgcggca tcaatgaaat aacggaagtc gtcgcgagta
    17461 ccaaaacggc gggttggtgc atacaggccg gttggctgat aaccccaact gccatcgaag
    17521 ggatgctcgt taatgggcag tagttcgagg tgggtaaagc ccatccattt agcataaggc
    17581 accagttgat cggccagctc gcggtagctc aaccagaaat tgttatcggt gtggcgacgc
    17641 caggagccca ggtgaacttc ataaatagag attggcgcat caaactgatt cgcttttttg
    17701 cgctcttcag tctgtacaac cttttccggc agcccgcaaa taagagacgc ggtttccggg
    17761 cgcatttgcg cttcgaaggc ataagggtcg gacttcagac gcaagttgcc attggcatca
    17821 atcatctcgt atttatagag ctgaccgtta tgcgccccag ggataaacag ttcccaaatg
    17881 ccgctctctt tacgcagacg catcgggtga cggcgaccgt cccagtagtt gaattgccca
    17941 accaccgaga cccgacgggc gtttggagcc caaacagaga aacgcgtacc tgtgacgcca
    18001 tccatagtat ctgcatgcgc gcctaaggtt tcatacgggc gcaggtgagt accttcagat
    18061 aatagccagg catccatttc ctggattagc gggccaaaac ggtaaggatc atcaatcagg
    18121 ttttgctgac catgccagac aacagccaac tgatagcgga aaaaattctt acgtcgcgga
    18181 atgacaccgc taaagaatcc ccgtgagtcg agacactcca gttttgcgag tttgcgcccg
    18241 gttttcggtt caatcaccca cacatcggta gcgtcgggta aaagggcacg gacttccagt
    18301 cccgcggtgg ttttatgcat tcccagtacg gaaaaaggat ccgcaaaatg gcctgcaatt
    18361 agcgcgttaa tcacgtctct atcgatacga tcggacatgc ttgtcttcct gttttattgt
    18421 gtcaccccat ccaattggat ttttgacttc tggttgtgac atttttttga cctgaacggc
    18481 gcagcactct gtgcatcctc tctgcgtcgt cctcacttca ggtaaggctg tgaatactca
    18541 tgtattcagc cacccttaaa gaatagccaa tgctctattt aactcccggt aaatcatgaa
    18601 acatctgcgc ttactcctgt attacgcact aacaggggcg gcatcgcgcc ccagatttaa
    18661 tgaataaaga ttacgccagt tgacgaagca tccgacgcag cggctccgcg gccccccaca
    18721 gcagctggtc gcccactgta aaggctgaca ggaactctgg tcccatattc agcttacgca
    18781 ggcggcctac cggcgtggtc agcgtgccgg taacggcagc tggggttagc tcacgcatag
    18841 tgatttcccg atcgttagga acgactttcg cccacggatt gtgcgcagcc agcagttctt
    18901 ccacggtcgg aatagacaca tcttttttca atttaatagt gaatgcctgg ctgtggcagc
    18961 gcaatgcccc gacacgcaca cataaaccat ctaccggaat tacggaagat gtgttgagga
    19021 tcttgttggt ttccgcctgc cctttccact cttcgcggct ctgaccgtta tcgagctgtt
    19081 tgtcgatcca cggaatcagg ctacccgcca acggcacgcc aaagttatcc accggcagct
    19141 caccgctacg ggttaaggtt gtgactttgc gttcgatatc gagaatagca gaggacgggg
    19201 tcgcgagttc atctgccaca tggccataca gatggcccat ctgggttaat aactcacgca
    19261 tatgtcgcgc accaccgccg gaagcggcct ggtaggttgc aacggacacc caatcaacaa
    19321 gatcattggc gaataaacca cccaacgaca tcaacatcag gcttacggta cagttaccgc
    19381 caacaaaagt cctgatgcca ttatttaatc cgtcggtaat gacgtcctga ttgacggggt
    19441 caagaatgat gatggcgtca tctttcatgc gcagagacga tgctgcgtca atccagtaac
    19501 cttgccatcc gctttcacga agctttggat agatttcgtt ggtataatcg ccgccctgac
    19561 aggtcacaat gatatcgagg gcctttagcg cctccagatc aaaggcatcc tgaagtgtgc
    19621 cagtggttcc gccaaaagac ggcgcagcct ggccaagctg agaagtagaa aagaagacag
    19681 ggcgaatggc gtcgaagtcg cgctcttcaa ccatgcgttg catgagaacg gagccgacca
    19741 taccgcgcca gccgataaaa ccaacatttt tcataagcgt ttttttcctg caaagatgtg
    19801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    19861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    19921 tgttagcacg cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    19981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctac ctattttcat
    20041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    20101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    20161 tagcctacga gcagaaaccg tctccatttc tggcggcatc attctgtttc tgatcgccat
    20221 taaaatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    20281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    20341 gttgttgtct catcagtacc cgaatcagat ggggcatctg gtgattgctc tgctgctggc
    20401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    20461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    20521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa ggttcaaaca ggtgctgcac
    20581 acaaccagac gcggcacctg ttttattacc tgaactgata atgaccttcg cccaatcgga
    20641 taacccttcc ctcttcttct gcgcggttca gccacacact aatttgttcg atggttaaac
    20701 cagtacaaga cgacagcctt tcaatactta tcgattcctt tgcaaagatc gccagttccg
    20761 ccacaaagag ttgataaaaa tcgacaggcg tatgcgcggg cattactgcg taatttgcgt
    20821 catcttcatg cagcgaaaac agttctggtt gagcgttacg gagtgctctg tcatacatca
    20881 gcccaggaac gtctgacagt ctgatcggca cattctcagc ctgttcagca ctccatgacg
    20941 ccccattttc tactaatcgt gaattggctg attgcatatc ctgatttggt ttaacctgca
    21001 caggcagcca ctgatgtttt aatgcctcta tcgccccggt tatcgttcca ccggtcattc
    21061 ccgcacgtac aaccattgcg ctttcagcaa ggcaataaat atatttattt cgcgccatcg
    21121 cattgccgac ggtgaaacgc acctctgggt aaaacggcga aatcaacacc aggttgcctg
    21181 ctataagccc ttcacgccat ttcactaacg tactcgtttt taacaagcta tcagctaata
    21241 cgccaacggc agttcccccg gcctccagtg ccgacgccat tgcacattca tcaatacctc
    21301 gcgcaccacc agagataacg caaatcccct gttgggccag tttcgcggcc agttgttggg
    21361 tatagcgcaa atcgtcagtc ggagcatctc gcgagccaac aatcgccata ccttccgctt
    21421 gcagtaatgc tttattcccg cagccaaata aaacgggagg tgcatccgtt cgcaatcggt
    21481 ttttcaagcg aacgggatac tcagcatctc ctcgggttat gatccataaa ccggcgcgtt
    21541 gccacttatc taccgccaac gccagactat gaccgcgcgc cagcaagcca agaatacgct
    21601 cctgagaaat acgcggatcg ctccagcctg taagtttctc ttgcggttgc ggcaccagaa
    21661 gctcggcggg actgatacgt tggtgcttca gccataatgc aaacttcccc cattcactat
    21721 tactaagagg tttatgctca ctcgccgccg cgcgagaaaa atcgctggtt agcaggagag
    21781 tcgcttgtgc attggctgaa agattcatca attttttacc gaggtagaag caagggcaag
    21841 aggataaacc gtcgggcaac ctgcctggcg taacagtgct gtcagaactg tcagcgtcca
    21901 tgcagaatcc acgatatcgt caaccagcaa cgccggacct ggcatcaaag gaggggtaat
    21961 cacaaacgcc ccgtcgagat tttgacactg gtgaaaacgg ttttgctgca ttttctgcgg
    22021 tggattgtcc acgacttttt caatggcatc aataaaaggt aagccaagtt tcgccgccag
    22081 tcgccgggca aaatcaggaa ccaggtcgag gtgccttaat gaaggaacgc agcataccca
    22141 ggttggttca ggatgcggat tccagcgttg gcgaaccatt tccgcacatg cctccaccag
    22201 ctcatcactg aatcgccccg tttttttctc ctgtgctacc tgctttcccc atccggaatc
    22261 tccccaacga gaaaggactc gccccgtaga tccttgtaaa cccgcaggca agttcccttt
    22321 aaatccgtat tgcgtaaaag ctccagatgc tacctgttta ttgagattta atggcaggtc
    22381 agcgtgttta ataaataaat ttgccgcatg gagtaagccg ctatcaatat cgggcgacaa
    22441 caacggatgc tgaagacaac tgctacattt accacaacgt tcggcacttg gctcatcgag
    22501 tgcgcggcgc agaaattgca ttttgcactc cttggtcgtg atatagcttt caacatctgc
    22561 cagctcattt tttctctgat tcatcaaatg gttaattcgt tcatggtcag gagagaaact
    22621 gacgatagtt ctgcgccata atttctcggt atacaccaca ggcgatggat tttcagctac
    22681 caataatttc agtgcttttt ctatttgccc ataacggaga ttcgtccgtt gttcaatgcc
    22741 tcgtagggta agaccgtcat tctcgctaag tacgttgagt atttcatgaa tttgcgcctc
    22801 cgcaggaaag gcgctttcac ggaagaattt atgaatagcg cggtcttcac caccacaaag
    22861 caatatgcca acagcggaat ctatagcacg cccggcacgc cctacctgtt ggtagtaacc
    22921 gacaatagac cccggcatct ggtaatgaat aacaaacccc aaatccggtt tgtcaaagcc
    22981 cattcccagc gccgtcgtcg cgaccagaac tttaatttta tttgccagca acgcctgctc
    23041 cagatattcc ctggcagtat tagaatcttc cgcgccttca caagtcacgc cgctgtagta
    23101 agcaaatgca ctgataccat ttgtccttaa ccactcggcg acaaggtcgg catcgcgagt
    23161 agtcagggta taaacaattc ccgatttgga aaactgaggg ataaccgttg ctaaccatgc
    23221 cagacgggat gactgttcac ctaagactaa ggcatcaagc gccaaacttt cgcgagccag
    23281 cgttccacgc tgaatcacaa tgtcacccaa ttgctgacgg atatcctcaa cgacacggtt
    23341 attcgctgtc gcggttgtac ccagaactgg ggtattcgca ggcagttggc gcaaaatatc
    23401 taatatacgt cggtaatccg ggcgaaaatc gtggccccaa tcagagatgc agtgcgcctc
    23461 gtcgaccaca agcaagccaa tacgatcggc tataggatat aaaaccgttt cgataaagtc
    23521 ttggtttgcc aaacgttccg gggaaaccag caagcaatcg acctcacctt gcagcaactt
    23581 atcgctaata cgctgccatt cttccctgtt tgtagaattc aacgtttcag ccgtaatacc
    23641 taaccgttca gcggcggcaa cctggttacg catcagggca agcaaaggag agatgataat
    23701 tgtcgggcca gcaccgcgat cgcggaagat tttactggcg atgaagtaca cggcactttt
    23761 cccccagcca gtgcgttgga caaccagcag ttttcgtcgt tggttcacca ccgcgtctat
    23821 ggcttcccat tgcccttccc tgaaagtagc ggtggtattt cctaacatcc gctgtaaaag
    23881 caattcagct gcgtgctttt ccatctttat ctcctgcgat ctttaggcag tgattagaac
    23941 ggattattag caggtaaatt tgcgagaggg atcaaaaaga tgaagagagg agataagaga
    24001 aatgaattac tgcgagcaag ctcgcagtaa tttgtcgcag taattaactc aacagctgga
    24061 acgcaatcat cccaacgatg gcaccgacag tgccgaggat ggtttccatc atggtccagg
    24121 ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc gaacaaccag aaaccggcgt
    24181 cgttaacgtg gctgacaaca atcgaaccac cagcgataca aatcgacagc gccgccattt
    24241 gcgcaccgga gtagttcagt tgttcaataa ccggcatcac cagtcctacc gccgttaaac
    24301 aggctacggt ggcagaaccc tgaatgatgc gcactgcagc ggccagcacg aagcaggtga
    24361 tggctatcgg caggcccatg ccagttaacg cttcgcccag tgccggacct acgccagagt
    24421 caaccagcac ctgtttgaac acgccacctg caccaatcac cagcagaatg atccccgccg
    24481 gttgcagcgc gtgaccgcaa atctccatca ctttgtcttt tggcatgccc tgacgcattg
    24541 ccaggccata aatcgctacc agacaagcaa ccagaatcgc ggtaaacgga tgaccaataa
    24601 actcgaacca ttcgtaagcg gtagagcctt ctggcacaaa acgcgcggca atggttttca
    24661 gccccaccag caccagcggc aacaggatca gcgacaggct gaatccgaaa gatggcattt
    24721 tgccttcgcc gagatgcggt tcgctgatgt cgtcaggaat atgcaactcc acgtagcggc
    24781 tgatgaaatt accccacagc ggcccggcaa taatcattcc cggaattgcc gcacacaggc
    24841 caatcaggat catccagcca aaatcggcgt tcatctgcga tgccagcagc attggcgctg
    24901 gccctggcac caggaacgcc gccgctgccg ccacgcctgc gaataatggg attaccagct
    24961 tcaccaggtt cgtaccggtg tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa
    25021 tcgccacttc aaagaacagc ggtagcgcac agaccagccc cgcaaggccg atggcataat
    25081 gcgcgcggct gtgaccgaag gatttaagca ttttgacggc aatctgatcg actgcgccgg
    25141 tttcatgtaa gatcttgcca aacatagctc ccagggcgac aaccaccgcc aggaagccga
    25201 gggtgcctcc catccctttt tccatcgtcg ctgcgatttt atcgagcggc ataccggaaa
    25261 aaaggccagc ccccatggac accaccatca aagccaggaa agcgtgcata cgcgccttca
    25321 tgactaaaaa cagcagcagt aaaacagacc ctactgctgt taaaacaagc gttaatgtag
    25381 tcactactta tttgcctttt ttaataacct caatggtgct tgccacaaca ccttccagcg
    25441 gttgatcgat atccaccacc agtacatcag tttcgtccgc acccggctcc tgcagcgttt
    25501 caaactgcgt caccaacatt tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc
    25561 tttcaatcac gtcaaaatcg cctttcaggt agatgaaaga gagattcgga ttaccttcac
    25621 gcagcaagtc gcgatagtgt tttttcaatg cagaacagac gatcagtgac actttattag
    25681 tgcgctgcat agcaaacgcg gcgtcgttca gcgcctgcaa ccacggtttg cgatcgtcgt
    25741 cattcagcgg ttcgccagac gccatttttt cgatattgcg ccgtggatgg aggaaatcgc
    25801 catcaagaaa cgcggcatga agttgatgcg ccacttcact ggcgaccgca gatttgccgc
    25861 tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg
    25921 tgcccccaca atacaagaat taaaaatgtt acgggtaact gttatcggta acattgtcca
    25981 gccggacaat atcagaagca atatccatcc gtgccttaag tgtataagtg tgagctactt
    26041 caaatttgta ggcttaaata gatccgcccg gtgacaaggt gaaacctaaa tctaacattt
    26101 tcggtgtcac agattcgcca cgaatacgcg ccagcaggcg ttcagcgcca atactgccca
    26161 tccgctcacg cggcgtcagc acgctcgcta accgcggctc catcacctgg ccaatgtcat
    26221 gaccgtggaa accggcaatc gccatatcgt caggaacctt caatcccaga cgctgacatt
    26281 caaacgccgc gccgaccgcc aggtcatcgt tggtacagaa cacgccatcc agctgcggat
    26341 attcccgccg cgcctggcga atcagttcaa taccggaaga gtaagaagaa gattgctcaa
    26401 ccatcacgct atacggcacc agacctgcat ccagcatcgc ctgttcgtat cccttctgtt
    26461 tgatgatagt acgttcgtcg agacgcgcgc cgagataggc aatgtggcaa tgcccgcgag
    26521 caataatggc ggtggtcatc tggcgtgccg cttcaaagtt atcaaaaccg acggcgatat
    26581 cgaggcacgg cgactggctg tccatgagtt ccaccaccgg gatccccgcc acttcaatca
    26641 tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat cagtccgtcg atattccatg
    26701 agagcatgga ttcgagacgt tcttgctcca tttccggttt ataaccgtag tgcgccagca
    26761 tggtctgata accgtgcgcg tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa
    26821 cctggttggt gagagaaggt aacaggacgc caatcgcccg gctggtggcg ttagagagga
    26881 tatcgggcgc acgattggga atatagccca gttcatcaag agcggcggca atcttgccgc
    26941 gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg gctgaccgtc attttggtca
    27001 cgcctacacg gtcagccaca tcctgaagta cgggtctttt ctttttcatc gtcctgaagg
    27061 tacaaaagag atagattctc ttagtttaac acggacgcat cagaaccttc ccggggaaaa
    27121 cagtgcttgt ttacaattat tagatataca ctaaatcaat tgcatcgcat tgtgctaacg
    27181 acgcggcaaa accccggaag cgtgcatcag cacgcgaccg gagcgagcga ggaagcctgc
    27241 aaaaaggcag gcttcaaaaa tgccgtggag ctttaaaccg gcggcagatc gaacagtaac
    27301 acttcgctgt cgctatccgc atggatggag attgcctgct catcccagat tgccagacca
    27361 tcgctggtcg aggctttcac gccgttaatg gtgacattgc ctttcaccac ctggatccag
    27421 acgcggcgtt cagcggcaat ctgatgcacc gactgctcat ctttcagcaa cgcccagcgg
    27481 tacagttcca tatcctgatg cactttcaat gaaccatctc gcgcatccgg cgagagcacc
    27541 agctgtttgc cctgtacggc atcgaagcga cgctgttcat aacgcggcgt aataccgttt
    27601 tcttcaggca tgatccagat ctgatacaga tgcagacgct cggtgctgct tgggttgtac
    27661 tctgagtgac gaatacccgt acccgcactc ataatctgga actcacccgc cggaacctgc
    27721 tctttattgc ccatgctgtc ctgatgctca acagtacctt ccagcacgta ggtcaaaatt
    27781 tccatatctt tatgcgggtg agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta
    27841 atcacgcgca gcgcggagaa gcccataaag ttcggatcgt aatagttggc aaaagagaaa
    27901 gtatgccagg agtccagcca gccatgattt gcatgaccac gttcatttgc tttgcgtaag
    27961 tagatcattt gtattcgccc cctgaatgat ttcgatgggc ttagtgtgga cccgatctgc
    28021 ctgggatgat agagggtgat aattgacccc tctgttcaaa aaaattgaac tattcgaggg
    28081 gccatcaaac ttaattcgcg agagtcactg tggagggaga ggcttgctca aatccgcgtt
    28141 caaggatttc cagattggta agaacttcag attccttgac gtaatttggc gcaccgtggg
    28201 tgatggtttg atacaacgca tcataaacgc gcccgtaatc gcccatctcc ggcttcatct
    28261 cttctctgac cgtcacgccc tcgtcattga catactccag cacaccgacc gaatcatccg
    28321 ctgcgaatcc cggttcgccc ggcataatat tagccttcag gctggtttcc tgctggtcga
    28381 taccgtattt aataaacgaa cctttcttac cgtgaacgat aaatttcgga taatcgattt
    28441 tcaccagatg gctggttttg acgatggctt tcaggtcgcc ataaaacagt tgcgcttcaa
    28501 aggtgtcgtc aggattggct ttattacgca ggctgcggat gtcataagcg acgtgatccg
    28561 ggcgaccgaa cagagaaata atctggtcca tcgtatgcac accaaggcca taaaacgcgc
    28621 catcctgcgg cagcccaggt ttggtttctg ccaccgggcg gtaatagtca aaatggcttt
    28681 ccacttcaac aatctctccc aacttgccac tttcaatcgc ttttttcgct gtcaggaagc
    28741 aggagtcaaa gcgacgattc tgatacggcg tgacggtcag ccctttgctt ttcgccaacg
    28801 caaacagctc cttcgcctgg gcaagcgtcg gggtgaacgg tttttcgacc agcacatttt
    28861 tcccggcttc cagcgcgcgt ttcgcgtact cgaaatggct gtccgcgtgg gtgcagacaa
    28921 caaccagctt aacatcggga tcgtttagta cttcgtcgag atcgctggtg aaatggatat
    28981 gggaataaat gggagcctgt tcttccggct tcgcatggcg acgaaaaata tgcgcgacat
    29041 gccagctatc cttgcggtta agtacatacg gcagatggta acgggtggtg cttttgccga
    29101 agccaataaa ggcgcagttg atgaccatga tttcgtcctt tttaaggtgg ttatttacac
    29161 cttagcgcaa agcggacgtg gttcctactg gagtgcgcat aacgaacaca agcactcccg
    29221 tggataaatt gagaacgaaa gatcaaaaaa aaagccagca cccggctggc taagtaatac
    29281 tggaagcaat gtgagcaatg tcgtgctttc aggttctccg cgagggtctt cctgatcgcg
    29341 agacaataat aatcattctc attcgcactt gtccaacact ttttgcaaaa aaatgcattt
    29401 gactcgcatt tgaaagtcaa tgatgttgaa agggacattt accccaaaga ggacaaagga
    29461 atgagtgaga tagtaatacg ccacgcagaa acacgggatt acgaggccat caggcagatt
    29521 cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc ctcatccttc cgatcatatg
    29581 tggcaggagc gactcgccga tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga
    29641 gacgtcgtgg gccatctcac cattgacgtg caacagcgcc cacgccgcag tcatgttgcc
    29701 gattttggta tctgtgtcga ctctcgttgg aagaaccgcg gcgtcgccag cgccctgatg
    29761 cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag atcgcattga actaaccgtg
    29821 tttgtcgata acgccccggc aattaaggtc tataaaaaat acggctttga aattgaaggg
    29881 actggtaaga agtacgcatt gcgtaatggt gaatatgtcg atgcatatta tatggcgcgg
    29941 gtgaagtaag atagtgccct ttttctgaga tggaaaaagg gtgtcattca aaatcgacat
    30001 accttccttt aaggta
//
LOCUS       DAPPNJ010000037        29616 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000037 DAPPNJ010000000
VERSION     DAPPNJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.037"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="RPM73_001323"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="RPM73_001323"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDX6519700.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="RPM73_001324"
     CDS             complement(804..1352)
                     /locus_tag="RPM73_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDX6519701.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="RPM73_001325"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="RPM73_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX6519702.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="RPM73_001326"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="RPM73_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDX6519703.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="RPM73_001327"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="RPM73_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDX6519704.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="RPM73_001328"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="RPM73_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDX6519705.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="RPM73_001329"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="RPM73_001329"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDX6519706.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="RPM73_001330"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="RPM73_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDX6519707.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="RPM73_001331"
     CDS             complement(11782..12675)
                     /locus_tag="RPM73_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDX6519708.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFRRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="RPM73_001332"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="RPM73_001332"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDX6519709.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="RPM73_001333"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="RPM73_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDX6519710.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /locus_tag="RPM73_001334"
     CDS             complement(13806..15038)
                     /locus_tag="RPM73_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDX6519711.1"
                     /translation="MSLPHLSLADARNFHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="RPM73_001335"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="RPM73_001335"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDX6519712.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="RPM73_001336"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="RPM73_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDX6519713.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="RPM73_001337"
     CDS             complement(17843..20107)
                     /locus_tag="RPM73_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDX6519714.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="RPM73_001338"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="RPM73_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDX6519715.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="RPM73_001339"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="RPM73_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDX6519716.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="RPM73_001340"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="RPM73_001340"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDX6519717.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="RPM73_001341"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="RPM73_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDX6519718.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="RPM73_001342"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="RPM73_001342"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDX6519719.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="RPM73_001343"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="RPM73_001343"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDX6519720.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="RPM73_001344"
     CDS             complement(26972..27664)
                     /locus_tag="RPM73_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDX6519721.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /locus_tag="RPM73_001345"
     CDS             27794..29554
                     /locus_tag="RPM73_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDX6519722.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7198 a         8056 c         7186 g         7176 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagttt ttcgttgaga tcgctgttac
     5161 cgctaagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttaactg ttcgaactgt tccgggtcgc tctgcaatac cgaaacaatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca gactgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgccct ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttacttaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgcagtc ggcggttaag tccggcagat ggcacagttc tttggcacga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgca cgagcctcat
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcatt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtagc tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtggcttcg ctgatcagat
     7321 gcttaaacag gtcgcggtcc gactgggtga cacgaatcgc ttccagcgtc atacggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcagtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 acggcttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgtgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta atccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgacgaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgcc ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagt tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcggggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt ccctgactgg tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcgacatcc tcatgatcgg ttgccacgat
    13441 gatgcgatcg gcacctgatt cgcgagcacg ttcaagaaca tgaacaatca tgggtttgcc
    13501 attaatatca accaatggtt taccgggcag acgcgttgac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcacg cagcgggaaa gccaggttgt ccagtttgca aataagttct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 agacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaaa
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccacc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa tgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat aacacttgcc tgcagaaagc
    19621 ggccggtgag cggttgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaaccgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc
    21661 cagagataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtaaatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggtaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgtttt
//
LOCUS       DAPPNJ010000038        29470 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000038 DAPPNJ010000000
VERSION     DAPPNJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.038"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            2..1291
                     /gene="uraA"
                     /locus_tag="RPM73_001346"
     CDS             2..1291
                     /gene="uraA"
                     /locus_tag="RPM73_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDX6519723.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTMI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1341..2087
                     /gene="hda"
                     /locus_tag="RPM73_001347"
     CDS             1341..2087
                     /gene="hda"
                     /locus_tag="RPM73_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDX6519724.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2225..2584)
                     /gene="arsC"
                     /locus_tag="RPM73_001348"
     CDS             complement(2225..2584)
                     /gene="arsC"
                     /locus_tag="RPM73_001348"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDX6519725.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2605..4068)
                     /gene="bepA"
                     /locus_tag="RPM73_001349"
     CDS             complement(2605..4068)
                     /gene="bepA"
                     /locus_tag="RPM73_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDX6519726.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4281..5342
                     /locus_tag="RPM73_001350"
     CDS             4281..5342
                     /locus_tag="RPM73_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDX6519727.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5380..6228)
                     /locus_tag="RPM73_001351"
     CDS             complement(5380..6228)
                     /locus_tag="RPM73_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDX6519728.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIASHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(6250..8262)
                     /gene="hyfR"
                     /locus_tag="RPM73_001352"
     CDS             complement(6250..8262)
                     /gene="hyfR"
                     /locus_tag="RPM73_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDX6519729.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNNLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8292..8705)
                     /gene="hyfJ"
                     /locus_tag="RPM73_001353"
     CDS             complement(8292..8705)
                     /gene="hyfJ"
                     /locus_tag="RPM73_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDX6519730.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMNFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8698..9455)
                     /gene="hyfI"
                     /locus_tag="RPM73_001354"
                     /pseudo
     CDS             complement(8698..9455)
                     /gene="hyfI"
                     /locus_tag="RPM73_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(9452..9991)
                     /gene="hyfH"
                     /locus_tag="RPM73_001355"
     CDS             complement(9452..9991)
                     /gene="hyfH"
                     /locus_tag="RPM73_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDX6519731.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAEQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(10001..11716)
                     /gene="hyfG"
                     /locus_tag="RPM73_001356"
     CDS             complement(10001..11716)
                     /gene="hyfG"
                     /locus_tag="RPM73_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDX6519732.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11706..13286)
                     /gene="hyfF"
                     /locus_tag="RPM73_001357"
     CDS             complement(11706..13286)
                     /gene="hyfF"
                     /locus_tag="RPM73_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDX6519733.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVMLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13291..13941)
                     /gene="hyfE"
                     /locus_tag="RPM73_001358"
     CDS             complement(13291..13941)
                     /gene="hyfE"
                     /locus_tag="RPM73_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDX6519734.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13953..15183)
                     /locus_tag="RPM73_001359"
                     /pseudo
     CDS             complement(13953..15183)
                     /locus_tag="RPM73_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(15200..16147)
                     /gene="hyfC"
                     /locus_tag="RPM73_001360"
     CDS             complement(15200..16147)
                     /gene="hyfC"
                     /locus_tag="RPM73_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDX6519735.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16158..18175)
                     /gene="hyfB"
                     /locus_tag="RPM73_001361"
                     /pseudo
     CDS             complement(16158..18175)
                     /gene="hyfB"
                     /locus_tag="RPM73_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(18175..18792)
                     /gene="hyfA"
                     /locus_tag="RPM73_001362"
     CDS             complement(18175..18792)
                     /gene="hyfA"
                     /locus_tag="RPM73_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDX6519736.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(19045..19515)
                     /gene="bcp"
                     /locus_tag="RPM73_001363"
     CDS             complement(19045..19515)
                     /gene="bcp"
                     /locus_tag="RPM73_001363"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDX6519737.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19515..20087)
                     /locus_tag="RPM73_001364"
     CDS             complement(19515..20087)
                     /locus_tag="RPM73_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDX6519738.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20233..21111
                     /gene="dapA"
                     /locus_tag="RPM73_001365"
     CDS             20233..21111
                     /gene="dapA"
                     /locus_tag="RPM73_001365"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDX6519739.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21128..22162
                     /gene="bamC"
                     /locus_tag="RPM73_001366"
     CDS             21128..22162
                     /gene="bamC"
                     /locus_tag="RPM73_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDX6519740.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22375..23088
                     /gene="purC"
                     /locus_tag="RPM73_001367"
     CDS             22375..23088
                     /gene="purC"
                     /locus_tag="RPM73_001367"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDX6519741.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23243..23470
                     /gene="vapB"
                     /locus_tag="RPM73_001368"
     CDS             23243..23470
                     /gene="vapB"
                     /locus_tag="RPM73_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDX6519742.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23470..23868
                     /gene="vapC"
                     /locus_tag="RPM73_001369"
     CDS             23470..23868
                     /gene="vapC"
                     /locus_tag="RPM73_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDX6519743.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24015..24878
                     /locus_tag="RPM73_001370"
     CDS             24015..24878
                     /locus_tag="RPM73_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDX6519744.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24893..26908
                     /gene="tmcA"
                     /locus_tag="RPM73_001371"
     CDS             24893..26908
                     /gene="tmcA"
                     /locus_tag="RPM73_001371"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDX6519745.1"
                     /translation="MAELTAFHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            26982..27680
                     /gene="ypfH"
                     /locus_tag="RPM73_001372"
     CDS             26982..27680
                     /gene="ypfH"
                     /locus_tag="RPM73_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX6519746.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27761..27961)
                     /locus_tag="RPM73_001373"
     CDS             complement(27761..27961)
                     /locus_tag="RPM73_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDX6519747.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(27989..29116)
                     /gene="dapE"
                     /locus_tag="RPM73_001374"
     CDS             complement(27989..29116)
                     /gene="dapE"
                     /locus_tag="RPM73_001374"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDX6519748.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29120..>29470)
                     /locus_tag="RPM73_001375"
     CDS             complement(29120..>29470)
                     /locus_tag="RPM73_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDX6519749.1"
                     /translation="TLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNGFI
                     NELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPMLL
                     GFSDSSYQQFFHEV"
BASE COUNT         7106 a         7698 c         7923 g         6743 t
ORIGIN      
        1 tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga caatcccgct
       61 tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg tcttatttca
      121 tattaacccg gcgactgtac tgttatttaa cggtattgga acgctgctgt atctcttcat
      181 ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt caccggtatt
      241 gttactgttg ccgttagggt atgaagtcgc gctgggcggc tttattatgt gcggcgtgct
      301 gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg acgtgctgtt
      361 tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg cgggcgtagc
      421 tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca aaaccatcat
      481 catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgtttc gtggtttcct
      541 ggcaattatc ccgattttaa ttggcgtgct ggtgggctac gcgctctctt tcgcaatggg
      601 aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccaa ccctctatac
      661 gccgcgcttc gagtggtttg ccattctgac tattctgcca gcggcgttag tggttattgc
      721 cgaacacgta gggcacctgg tagtaacggc taatatcgtc aaaaaagatc tgctgcgcga
      781 tccaggtctg caccgttcga tgtttgctaa cggcctgtcg accatgatct ccggcttctt
      841 tggctctacg ccaaatacca cttacggaga aaacattggc gtgatggcga tcacccgtgt
      901 ttacagtacc tgggttatcg gtggggcggc gattttcgct atcctgcttt cctgcgtcgg
      961 taaactggct gccgctatcc agatgatccc attgccggtg atgggcggcg tttcgctgct
     1021 gctttatggt gtcatcggtg cttccggtat tcgtgttttg atcgaatcga aagtggatta
     1081 caacaaagca cagaacctga tcctgacttc cgtgattttg atcatcggcg tcagtggcgc
     1141 gaaggtaaac atcggcgcgg cggagttaaa aggtatggcg ctggcgacca tcgtcggtat
     1201 cggcttaagt ttgatcttca aactgattag tgtgctgcgc cctgaagaag tggtgctgga
     1261 cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt tgctgcccgg
     1321 ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg
     1381 tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac gacgaaacct
     1441 ttgcaagttt ctggccgggg gataactcct ctttactggc tgcgctgcaa aacgtgctgc
     1501 gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg cgcagccatc
     1561 tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc
     1621 tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat ttgtcgctgg
     1681 tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg gcgattttcg
     1741 atctctacaa tcgaattctg gaatcgggca aaacacggtt gttgatcacc ggcgatcgtc
     1801 caccgcggca gttgaatctg ggattaccgg atctcgcgtc gcgactcgac tgggggcaga
     1861 tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag ttacgcgcgc
     1921 gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag cggctcgaca
     1981 gagaaatgcg cacgctattt atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc
     2041 aacgtaagct gaccattccg tttgtgaaag aaattctgaa gttgtagata aggtgtttat
     2101 tgtcggatgc ggcgcgaacg ccttatccga actacgcgaa gttaatccat agtattcgta
     2161 ggccggataa gacgtggcaa gcgtcgcatc cggcaatgtg cttcaactag cgatggagca
     2221 catcttaacc cacgatttcc agtacctgct ccggtggacg accaatccgc gctttgccat
     2281 tcgcgaccac aatcgggcgc tccattagct tcggattatc caccattgcc tgaatcagcg
     2341 cctcttcgct aagcgaactg tcagccaggt tcagttcttt ataaagatcc tctttctggc
     2401 gcatcaattc acgggcgcta ttcatcccca gcattttcag caaatcgcgc agcgttgccg
     2461 catcggcggg tgtctcaagg tagagtacca cttctggctc cacgccgttt tctttcagca
     2521 ggttcagcgt ttcccggctc tttgaacagc gtgggttatg gtagattttt acctgtttgg
     2581 tcattgttct tcctttaatg cgaattacat cttggtataa ggcttaaagc gttcctgcag
     2641 ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt tgcaggctgc ctaatttcac
     2701 ctgcgaactg gcgctactca acagcgaaat ggcctgatcg agtcgtccgg cgagcgcata
     2761 accttctgct cgcgcagcca gctcctgatc gcggttattt agcgcggctt ccgcctgtgc
     2821 cagtaaatcc cagccgttgc tgtcatcttt attgttaaaa gtgtagcgat tgagaatatt
     2881 ggccgcttct tgtggttgac cgccttgtag ataagcgttc gccaggttga gctgcaacac
     2941 cggattggtg cgcaaatcac gggcattttt caggcgattg attgcctcat tggctttgtt
     3001 ttgcccaaga tcgatatcgg tagccagatc gagataccat gcgttgccag gttctgccgc
     3061 cagtaacggt tgcagcgttt tacgcgcctc gtcgtattta ttggcttcca tcgcctgtaa
     3121 agcacgacca tattgcgccg ctcgttgctg acgaacgttt cctttcgccc attcatccag
     3181 caaatcactg gtgagctggt tacgtccgga attatacatc cccagtgtgc gcgctttcgc
     3241 cagatagaaa tcttccgacg actgcaccac catcggtcgc atctgattag cacggttgcg
     3301 ggcatctgcc agacgacttt ccggcaacgg gtgagtcaat aaaatttccg gcgggcgcga
     3361 ggagtaacgc gcctgatcga gtaatttttc gaggaaggtt ggcatcgcct gcggatcgaa
     3421 tcccgagcgt tgcagcacct gaataccaat gcggtccgct tcctgttcat tttgctgggt
     3481 gaaactgatc atcccctgac gtgttcccgc cagtgtaccg gtcagcgccg ccatccccgc
     3541 ctgcggactg gccatcgcca gtaaaataga acctaacgcg ccgacccagg tcagcggcgc
     3601 gctgcgttgt tgatcttcca tcgctcgcgc caggtgacgt tgggtgacgt gggagatttc
     3661 gtgcgccata actgaagcca gttgactttc gttatcggaa taacggaaca gggcagagtg
     3721 cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt tcgtcgttgt tgatcagaaa
     3781 aaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc agacgcatcc ccagcgaatt
     3841 aatatattgc gttaacagcg ggtcattaat taacggcgcg ctgccgcgta gctggcggac
     3901 ataatagtcg cccatctgca tttcctgacc aatggaaagc gtgcttcctg cggaggttcc
     3961 catatccggc aaggtgtctg cgctgtcggc aaacgccggg gctacctgac caatggtcat
     4021 agcagcaatg agggttgcaa ccaggttttt tttcaactgc ctgaacataa cctctatcct
     4081 gtatttctga agatagtcat ttgaccgata ctgttaaata tcgttcccac cgcgccgcct
     4141 ttgagtgtaa cggcgaagat tagcccactc aaatgacttt tcagtttcct gaatccgtca
     4201 taaaaagcga agtctttttt gtgacatttc gatacaattg cggatcgcaa tcccgctctt
     4261 gaggttcagg gaaggtcttt atgctcgaaa tgttgatgca atggtatcgc cgccgtttta
     4321 gcgacccgga agcgattgcc ttgctggtta ttttagttgc cggatttggc attatctttt
     4381 tctttagtgg cctgcttgct ccgttgctgg tggctattgt gctggcctat ttgctggaat
     4441 ggccaaccgt gcgcctgcaa tctattggct gctcccgccg ctgggcgacg tcgattgtat
     4501 tggtggtttt tgtcggtata ttgctactga tggcgttcgt ggtactgcct atcgcctggc
     4561 aacagggcat ctacttaatc cgcgatatgc cggggatgct caataagctt tctgactttg
     4621 ccgccacgtt gccgcgccgc tatccggcgt taatggacgc gggcattatt gatgcaatgg
     4681 ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc ggtggtgaaa atttccctcg
     4741 cctcgctggt cggtttgctg accatagccg tctatctggt gctggtgcca ttgatggtct
     4801 tcttcctgct gaaagacaaa gagcagatgc tgaacgccgt tcgccgggtg ctgccgcgta
     4861 accgtgggct ggcaggacag gtgtggaagg agatgaatca acaaatcacc aactatatcc
     4921 gcggcaaagt gctggagatg atcgtggtag ggatcgccac ctggctgggg ttcttgctct
     4981 tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg cttctcggtt cttattccgt
     5041 acattggcgc atttgtggtg accattccgg tggttggcgt ggcgctattc cagtttggcg
     5101 caggcacgga attctggagc tgcttcgcgg tgtatctgat tattcaggcg ctggacggca
     5161 acctgctagt accggtgttg ttctccgaag cggttaacct gcatccgctg gtgattattt
     5221 tatcggtggt gatcttcggt ggtttgtggg gattctgggg cgtattcttc gccattccat
     5281 tggcgacgct gatcaaagcc gtgatccacg cctggcccga tgggcaaatc gcgcaagaat
     5341 aatcttcagc ccgacgaact gtttcgccgg gctgattttt caggattcct gacgtaaata
     5401 aatagcccga taacacatac tcaccagcac tgcaccgccg ataatattac ccagcatcac
     5461 cgggagcaga ttggcggtaa taaaatggct gaccgtcagt gccggaaaat tgtctgcgct
     5521 actgtgcgcc agctgccaga aggggggagg ggcgaaatgg cgaatggcaa tggcgaacgg
     5581 aatcacaaac aaattggcga tacagtgctc aaagccactg gcgacaaaca gggtgatggg
     5641 caaaatcatg gcgacgattt tgtcgcataa tgaacgcccg caataactca tccacagcgc
     5701 caggcaaacc atcagattgc acataatgcc gaggctgaca gattcagtaa atgtatgatg
     5761 cattttgccc tcggcgcagt gtaaaaccgc gactccccac atcgcgtttt cactcatcac
     5821 cagcccggaa aaccagatta acaaactgaa acaggcaata cctgccagat taccgcaggc
     5881 caccagaagt gcgttaatca gccaagttcg ccaactaata acgccccgac tttttgccat
     5941 taccgtcatt accgacgagg tgaacaggtt ggtgccgcaa accgccagca aaataaagcc
     6001 aagtgtaaag caaaggccgc ccaccagatg ggttaatgcc tgtgaagacg gggcatcggc
     6061 gataactgaa aggtaaaaag taaatccgat cgccataaac acgccagcgg acatcgccat
     6121 taaaaagacc gacactttac tgcgggcaat tttatgggag gcaatccgtt cagcgattgc
     6181 cgcttttctg gcgctcaact gcaagtcgaa agagagtttg tttctcatgc atactcctgc
     6241 aaaagcagat tacaacacct cgcgaaccga gatccccaga cgctgcattc gtgacagcag
     6301 cgtggtgcgc ttcatcccta atcgtgtcgc cgcgccacgg ggcccggcaa caatgccatt
     6361 ggtttctcgc aatacctgaa caatgcgctg acgttcttct tcgtcattct ctggcgtcgt
     6421 cgggtgcagc aactgcgcca ttgaactgcg aagcgctgaa tcttcattta gcgtcggcag
     6481 taaacggctt tgtcggacat ttagatgtaa attcagattg ttaccacgag tcaacagtac
     6541 cgcccgctca atcacgtttt ccagctcgcg cacgttgccc ggccaatccc acgacatcaa
     6601 ctggcgtagc gcctcggtcg ggattgcgtc aattgcgcga ttcatatggc gcgccatttt
     6661 ttgcgtgaaa tgctttgcta aaagagggat atcttccgga cgatcgcgta gcggcggcaa
     6721 ttccagtggg aagacattca ggcgataaaa gagatcgctg cgaaactggc gatcttcaac
     6781 catttgccac aaatcacggt tggtggcggc aatgactctg acattcaccg ggatcgttct
     6841 actcccgccg agacgctcaa tctcccgctc ctgcaatacg cgcagcaatt taggctgaag
     6901 ttctaacggc agatcgccaa tttcatcgag aaacaacgtg ccgccatcgg caatttcaaa
     6961 acgaccacga tgggtattaa tcgcaccagt aaacgcccct ttgtcatgac cgaataactc
     7021 actttccaga agactggcgg ggatggcagc gcagttgatt ttgaccagcg gcttgtcgcg
     7081 tcgcgggcta agttgatgga tcgctctggc gatcacctct ttgccggttc cggtttcacc
     7141 gcaaatcaac accgtactgt cgctcttcgc cacaatatct acctgctgga gtaggtcttc
     7201 cattgcctgg ctttgataga taatgtcacc gatgcccaga ttcgaaagga gctgctcgct
     7261 aagctggtgg ttttcttgct gcaaactttc ctgcaaatcg gtcatgctac gccaggcatc
     7321 ggcattgcca acggcaatag cgatgcgatc ggctatgtgt tgtagtagct ggcagttttc
     7381 ctcactaaag agagtggaag aggtatgcgc cagcaacaat gcacccggtg tatggttgcc
     7441 aaaggtaagc ggtataagga gcgcagattc gcagccgtta gatgcaagta agagtaataa
     7501 cgggtcgcgt tgccagagaa acagatcgtc tgcacggtgc gtcaaggtcg gttgtcgggt
     7561 ttgtagcgtt tgtgtcagca atacactgtc gccaggcata ttgcgtggca gacacgcaca
     7621 atgtgaggca gaaagatcgc tgcaccacag gctaaacttc tcgttctttc gatgatcgcc
     7681 cagtaccata ctgactgaag ccagaccgaa aaaatgatgg atctcacgag cgacgtcagc
     7741 gatcagatcg tcgagatcaa gatgtgacag cactgcatta gtaatatcaa ccagtacccg
     7801 atgatgatcg cgatctttct ccagcaaacg cagcgccacc tgctgttctt catcattgag
     7861 ccatgattgc agaaccgggg tgattatccg cgccagccag ttaaacagtg tgcaatcctg
     7921 gggagtaaaa cggctgccgt gggtacgaat aaagcgtagc tccgccattc cactactgcc
     7981 gagaaggacg gcgtgacgcg aataatcgac catctcgccc tgatactctg ttgttgccgt
     8041 cacatcgctg ccatcatcat ggtagcggta atagaagttg cttttgagaa catgcagttc
     8101 aataagttgt accgggctga acggcggcgg catcgggatg aaggcgcgta ataaagacgc
     8161 cttaccgtgt ttctgtgcta accgctccgc gagcattccg tttaccgctt caattgttat
     8221 tccttgtggc ggggcaaaca tcgcctcgtc tgacatagcc atcaacagtc cttattaatg
     8281 agaaatcctt ctcacgcaat ttttctggcc atcagataga tggccggttc ctgcacaatt
     8341 tcatccaaca gatgaatgaa ttgaatgcta cgcgcctgcc acacagacgt cgcgtcatcc
     8401 gccagtggcg cgaatgcccg ggtcaacagg gcggtgtggc tggcgtcaat aacaatctca
     8461 ccaaaattca tcacccccag catcttacgt cgggcttgct cctcttcgac ctgggcaagc
     8521 caatcttcgt attccgtcag tgggcagggg aaggcgacat tcagacaatc aatcacgcca
     8581 acgtgatgac cgatagccag cgagtaatac attacctgag agctgtgttc cggcatctcg
     8641 tcgctactgg cgacaaactt ttttcgcagc gtccagaaaa caatttcccc gcactcttca
     8701 gtcatgtaac ccccggagta cgcgaaagag ttgctgaacg atactattga gacgtggatc
     8761 gtcggcatcg cgcaaccagg tgttaacccg atttcctgta ggatcgtcgc ttaaatggcg
     8821 caggagccga tcgcaaattt ctcgcccctg acgatagccc gccagccgcc gcgcttctcg
     8881 ctcaatggcg atacgctgtg atggcgggat ctgcggccac aacggttgca tagtcacccc
     8941 ggtgggatcg cgataatcca ccgcgtgaat cttctgttgc agcaaaccga gcgccacggc
     9001 gaaaccgtga atggtggcgg ccggtgttgg cgggcagccg gggatccaaa catcaatggg
     9061 gacaatggtg tcgctgccgc cccagacgct gtagagatcg tggaaaatcc gccgccggca
     9121 ccgcacgcgc cgtaggaaac acagatttta tgatcggggg cagactcata cgcccgaagt
     9181 gcaggcatac gcatcgcccg ggtgactgcg cctgtaaata acaaaatatc ggcgtgacgc
     9241 ggtgatgaaa taaccttaat gccaaaacgt tctgcgtcga atactggtgt gatggcggca
     9301 aaaatttcga tttcacaggc gttgcagccg ccgcaatcga cgcgataaac gtaagccgag
     9361 cgacggatat cctgtagcaa atgctgcttc atcttttgcg tttgctcgtc cagcgtgatg
     9421 ggctggctga catgttgtgt aagcactgga ctcatagctg ctccttagcc accagcggta
     9481 catctgtatc gtcgttgagc agcgtcgcgc gttgtttgca ctccgggcag acgctcgctt
     9541 gcgcccataa catttcgcgg ttttgtggcg cattttgttg ctctgccaac aattcagcag
     9601 ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca acggctgcaa cgttgtagat
     9661 ggaacgtcgc gcgggtatag agatcggctt tattggtgac ggtcagttca aagttattgg
     9721 taagctggat ggctctggtc gggcacactt cttcacaacg tccgcagtaa atacaacgcc
     9781 tcagatagag ctgccaggtg cgcgaatttt gctggtcgtc ggtctggata gtcagcgcat
     9841 ttgccggaca agcacaggcg ccgcaggcaa tacattggct gggcatcagg tccggtttcc
     9901 cgcgaaagcc tgggctgacc tccagtggcg cgaagggata tttcaccgtc gcggttccgg
     9961 cgcgcataat agttttcagt aacttcagca tcttctgtcc ttacttcagc ggcgaacggt
    10021 tacgatcaat gccgtagcgt tcgatctctt tgtacggcac ggtttttgac tggcgcttgc
    10081 gcacatctac cagcgtcaca cggtcggtac aggagtagca gggatcaagg ctaccgataa
    10141 tcagcggtgc gtcagaaacg gtattgccgc gcagcatgta acgcaacacc ggccagttgg
    10201 cgtaggtggc ggcacgacag cgccagcgga acaatttttg gttatcaccg agcatgctcc
    10261 agtgcacgtc ttcaccgcgt ggcgcttcaa caaagcccag cgcgaatgcg tgaggtttat
    10321 agctaaagcc ttcggtcagc agcggggtat ccggcatgtt gtcgagggca aattccagca
    10381 ttgccagcga atcaaacgtc tctttgacac ggaccatcac gcgggagaaa acatcgccgc
    10441 cggtgaaggt aaacagtgtt tttggaatat tgccgtagtc ggcgtagggg tgatcaaagc
    10501 gcaaatcacg ggcaaaacca ctgccgcgga tcagcggccc tacagggcta taatcacggg
    10561 cgatttgtcg gtcgagaatg ccaatgccct gagtgcgttg ttccatattc ggcatagcaa
    10621 gcagcatctc taccagctcc gacacgtcgg cgcgcatctc gcgcaccagt ttcagcgttt
    10681 gcagacgttg ctctttgaga atatcgcggc gaacaccacc aatcagattc agaccgtagg
    10741 ttttacgcga cccggtcagc aattccgcca tcgtcatcga cttttcccgc acgcggaaaa
    10801 attgcataaa gccggtatca aaaccaacaa aatggcagga gaggccaagg ttgagcaaat
    10861 gactgtgtag ccgttcgact tccagcagaa tcgagcgaat ggtatgcgct cgttgcggca
    10921 cctcaatccc cagtgcattt tcaaccgagt tggtataggc cacactgtgg gcaaaaccgc
    10981 aaatcccaca cacgcggtcc gataagaagg tcacttcgtt gtagcccatc cgcgtttctg
    11041 ccagtttctc catgccgcga tggacataaa acaggcggta atcagcatcg acaatttgct
    11101 cgccatccac aaacaagcgg aagtgacccg gttcatcgga ggtgatatgc agcgggccga
    11161 cagggatcac ccgcgcatcg ctgttgccct cattgataaa cgcatacgtt tcggaatctg
    11221 tcgtcggttc agggcgcagt cgataatcca tcgcatcttt gcgcagcgga tgcatatctt
    11281 ccggccagtc atcgggcaac accaggcgac gctgatccgg caggccaacc ggaatcagcc
    11341 cgtacatatc gcgaatttct cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg
    11401 acggaaactc ccgactgtcg gcatcgacca gtgccttcac cacgatccag catttttcgg
    11461 ccccttccat tgaaagggca taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt
    11521 tgccaaacag gactggaagc cagccatcat gttgataata aagataatgt acaacgtcag
    11581 gcagcagatt gattttcacc gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca
    11641 gcaccgcgcc ggggaactgc gttttcaggg cggcgagaat cgcttcgcca cgatttgacg
    11701 atgaattaac gttcactgtg tttctccgaa acagatgcgg tgcctgaagg caaaaaatca
    11761 tgccaggtgc tacgttgtgc aggcaggtcg tgcgtccctg agaggactat agtggaagcg
    11821 cccgccagga tcctgatgac aggttgtgga atatgcgttc ccatcgccag catcatgacc
    11881 agcagaatca ccattggcga ggtggtcaac cagccgagat caccccggtt aacggcctgc
    11941 ggcggtttcg ccattaacac ccgcgcagcc atccgtacca ggcccgccag caccagcgtt
    12001 aacagcaata acagcatgac gataatcagc aggtgattac gtgccagtcc ggcggtaacg
    12061 gtcataaatt cgctaagaaa aatgttgaag ggcggcatcc cggccagcgc cagcgcaccg
    12121 ccgccaaaca gcacggcggt aaatggcatg attttgagca tcccgcagac gacgttgaga
    12181 tcgcgcgtgc cgtacttgag cagtacattg ccggaaccgc agaacagcag cgtttttgcc
    12241 agactgtggt ttaaggtgtg cagcagcgcg gcaaaaattc ccagcggccc gccaatgcct
    12301 agcgccaccg cgaccagccc catgttctcc acgctggagt acgccagcag acgcttaatg
    12361 tcccgctgta ccagaatgaa aaatgccgcc acggcaaccg acaacatgcc gaagatgagc
    12421 aacaaccggt tggggaaatc gctgccgatg gcctggcaga taatgatgta atagcgaatt
    12481 agcaccaaca gcgcgcagtt cagcaatacg gcggagagca gggcgctgac cggactcggt
    12541 gcttcactgt gagcatccgg cagccaggcg tgcatgggaa atagcccggt tttggtgcca
    12601 aagccaatta gcacaaacac aaaggccaac agcattaatg ttgggtcaag caaggacgat
    12661 tgcttaagaa cctcgctcca gaatatcgcc atttctgcct gcggcataac gctggcgacg
    12721 ttggcgtata ccagcacggt accgaacaga ccaaaagcga caccaacagt acaaataatg
    12781 atgtacttcc atgctgcttc cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc
    12841 gagcttaagg tggtggcttc gatcgccgcc cacatcacaa tcaggttatt gctggtaaca
    12901 accagcagca tggtaaacaa aaacagatgg aagaaaccgt agtaatcgca cagcgtaacg
    12961 ggggaaagct cgccgtgtgc cacttcatga cgcatgtagc caatcgagta aacaccggtg
    13021 agaaagccaa tcacaccaag aattgcgagg aacaaaccgc ccagaccatc aatatgaagc
    13081 cacagtcccg cagcgaatat ttctcctgca tcagcggcag tttggaccac ccagagtgcc
    13141 agaatcagca gcagtgtgat ccctaagcta tgtaatacag tcaccgtaca agtcgcagag
    13201 agcccccgtt tccggcaggc aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt
    13261 aaagcgaaca tcacagaata actcatctca ttatcccttc agcgcggtca agttgttcac
    13321 gtccagcgtg ccgtgggtac gccatatttt tcttgccagt aacaccatca caatgacggc
    13381 gaagatggcg tcggtagcga tacctatttc caccagttcc ggtgctcgcc aggcaagaag
    13441 cgccagcacc agatgggagc cgttttccat caggcagtaa ccaaaaattt gccgcaggat
    13501 attgcgctgg ctgacaatgc acagcaggcc aagcagaaaa tgacctaacg ctaccgccag
    13561 cgccggtttc agcccggtag ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag
    13621 caggacaatt aacgccgcga gcagtgccat cattgccgga ccgaataacg ctttttccgg
    13681 gatgttctgg ggaatatttc gtgcagcgta agtcatgatt aacggtacca gcagcacttt
    13741 ggtgataaag gcgctggcgg accagatcag cagttgctct gcggcgaaca ggcacgagag
    13801 agtggcgaaa atagacacca gcaccagcga ctggcaggcg taaaatccgc atgacaggcg
    13861 atagcttttg acgctaatca caaacagcga tgtcagcatc atcagtcccg ccagattatt
    13921 tacgatcata gaaccggtca ttcatcatct ccttactgca accaggtcgc ggcgattacg
    13981 ctggaaatca gcgacatcac aatcagtaca atcaacacca ggcgcattga ccctggcagc
    14041 ggtgcggcat cggcgacggc ctcgctcggt ttgccaggca caacgcgacc aaaccagcga
    14101 ataaaccagg cgaaactggc gaccgattca atcatcagca gaatcatggc gggcagcagg
    14161 atccagtact ccactgacaa cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag
    14221 ccgttgaacg gcggcacgcc ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc
    14281 aacggcaggg tgtgcaatac gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa
    14341 ctcagcgcac ctgctacaag gaaaaacagg cttttagcga acgcgtggtt gacgatgtag
    14401 gcgatgctgc cctccagtgc cagccgccca ctttcaccat cgatgcggcg tggagatagg
    14461 cgctgatcgg tgttggcgct tccattgcgt ccggtagcca cgcttgcatc ggtagctggg
    14521 ccgatttccc ccacgcggca aacaggatcc cgccataaac cagataacgt gcgtcgccgt
    14581 gtaactcgct cattgcgcta agcgcaaacg ttccggtctg caaaaacagc gtggcggcgg
    14641 caagatacaa ccccaacgaa ccgatatggg tgataagcaa cgcttttagt gctgaacgct
    14701 gtgctttatc gctctggtaa taactgatca acgcccagga gcagccgccc taatttcaaa
    14761 aaacaacaac tgaccgagca gcgtcgagga gagtaccagt cccgccatcg cgccgataaa
    14821 caccagcaaa aatgcgtaat aacgattcgt gccgttatgc gggtgttcgc gatttttatc
    14881 cgtcagataa cccgtggagt agatcgtgac cagcaaaccg agaaacacca ccacaaacag
    14941 aatcagcgta ctcacgcgat caatgaccag gccaaacaat gccacatccc ccacgttgac
    15001 caatgtcaac gtgacggcaa ctttatcggc ctgataaaac gcggagatca gtgacaacat
    15061 gcacagcgtg gtcagcgcgg cgaacaaaac cccccattcg gcggcccgac gttgtggcga
    15121 aaacgaaacg accagtgcgc cgataaaagg cagcaataac gtcgtcagag caagattttc
    15181 catattccgt cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa
    15241 ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt
    15301 tttctgcgat tgaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg
    15361 aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc aaaggacaga aacagggcca
    15421 caaacagtga tgccatcacg acctgtttca gccccagccc ccatttcgct agcgccagcc
    15481 cggcaccgga atattcggtc agcgggcctt cctgtaattc ctgttctgct tcagcaacat
    15541 caaagggaat ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta
    15601 cggtggttag cggcgagttc cagcccatcg ccagcgtatt gctgatcatc tcgatatgcg
    15661 tggaacctgc tatcagcgcc aataccagca gtgagagaat aagcattggt tcgaccagaa
    15721 tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa cggacttccg gtatccagcc
    15781 cggaaagagc aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc
    15841 cgccgcccgc aaaaggggaa acggtaataa acagtggtaa ggccatcgcc agcaccagca
    15901 tgctgctgat taatacccac ggcatcaggc ggaacatcag acctgaagat gtcggcgcaa
    15961 cttcctggcg tttaaacagt ttgtggatat cgcgataatc ctgccagatc cccggcccgc
    16021 ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc cgtaaaaagt ggggttagca
    16081 tcagcagaat cacggcctgt gctaacgcaa aaataaccag atatccgtcg caaagagttt
    16141 gtctcatggt gatttcctta gacggcaata gcgattagca gcacaaccag tgcggcgacc
    16201 acatacaggc aatagagacg aaagtcgccg ctttgcagat gctgtatttc tttggccagc
    16261 cgttgggtgg cgctcacgat ggggcggatc acccgctcat cccagaaggg ttctgtgctc
    16321 tgagccctgg cggtgacgtg cgcaaaacct ttatttagcc tcagcgtagg gtcgagctgt
    16381 tgtcgtagac gaaataacgc agagaagacc acacgcagcg gctgcatcac gccattgcct
    16441 gacggggcca tcgcattttc ccagccgtag ccgcatgccc aggcatctcc tgtgcgacga
    16501 aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa aggcatcgcc
    16561 agtagtaaca gcaacagtaa tgagggggtg acctgtgtat gaaacgtgcc gggtacaagt
    16621 gctattccgc tggcgacagt gacgggaggg gtattggtaa acgcatgggc aatatgcatt
    16681 atcttcggtg ccagccaact ggcactaagc gcaattaata cgcagagtgc cgcgagcagt
    16741 agcatggcga cgatcatcgt atttggcact tcctgtgcct cttcagcgtg tgtactgcgc
    16801 ggcgcaccac agaaagtaat accgtacatt tttacgaagc acattactgc cagcccacca
    16861 gtgactgcca gcattaccat agcaatagga cccgcaagtt gtagcgctac ggcttccaca
    16921 cgacttagtg agaacagcga ctgccaggtg taccattcgc tgataaaacc attcagcgga
    16981 ggaatggctg atatcgcgag gcaaccaatc aggcatgctg cggctgtcca cggcatccgt
    17041 ttcgccagtg cccccatttt ttccatgtcg tgggtatgca aacgcgaaat aatcgcaccc
    17101 gcgccgagaa atagcagccc tttgaacagc gcatggttga gcagatgaaa cagtgcgccg
    17161 agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca gaccgtccat cgccacaccg
    17221 accgccagca aaataatgcc gacgttttcg acggtactcc aggccagcag ccgtttgata
    17281 tcctgttccg ccagtgcata cagcacgccc aggagcgcgg agattgcgcc gatcgccatc
    17341 accagagtgc cccaccacag aggcaaaccc gtttgcgcca gcagatccat cgctactttc
    17401 aggatgccga aaataccgat tttgaccatt acgccagaca tcaacgccga agcgtgcgat
    17461 ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg
    17521 ccaaaaccga aaaaggccag cagaaacacc gccgacgcca gccccggaga aagtgaaagc
    17581 gtgcgaaact ggcaaaatcg aggctgccgc tttcgcgccc catcagcaag aaggcgatca
    17641 taatcagcac cgatccggcg tgggcgataa aaaagtagag catgccagcg ttgatcgacg
    17701 ttttatcctg cctggcaatg accagaaacc aggaagacag cgacatcatt tcaaacagca
    17761 cgatgaacca aaaagcgttg tccatcacca gcagggcaac catcgatgcg atgaaaagat
    17821 tcataaagaa gcccatcgcc gccgcgcctt tgccctcgta ttcgcgcata taagtcaatg
    17881 aatagagcga acaaaccacc accagcaagg agataaccag caccataaat gccgagaggc
    17941 tatccattcg cagggttaaa tcggcaaagt caaaaggggt ggcaaaatgg gcgactaaag
    18001 ttacaccagc atgtaattgc gtaattccgc tgatgatgcc aatcacgccg cccaccagcg
    18061 atgtgatgcc ggaaagctta atagccagtc tgtccagacc gagtaaaaac agcgaagcca
    18121 gactggcaaa cagatagagg atcagcgacc aggttaataa ttgcagggca tccattagtg
    18181 ttgctcctga gtgagggaaa ggggatcctc cccgccattg gcaaaccagc ttgcggcaag
    18241 gcgctgtttt tctttcatct gacgttgcag gctatcgtcg gtgatcagcc gtaacgcctg
    18301 attcgggcaa gcgcgaacgc aggccggacc ttctggcaag aaatcacaca ggtcgcattt
    18361 caccgcgacg gtctggacac ccggttccca gcgcagcaac gcatgttggg gcagcacgtt
    18421 ttcttcgccg tctttgagtg agccttcagc ctgaaaaaca tattgcgcat gggcattcac
    18481 cggacggctt cctgaagcgc tgattgcgcc aaatgggcag accacggcgc aaagcttgca
    18541 gccaatacag aggctttcgt tgagttggat cgcatcatcc ctctgagaga tggcattgac
    18601 cgggcagacc tgcaggcaag gggcttcctc acagtgatga cacacgacag gggcagtgat
    18661 tgttgacgtc ttcgccaggg ccaggcgcgg gtgttgctgt aaaccttgcg ttttatgcac
    18721 gtccgaacag gcagcgagac aggtattaca tcctgtacac cacagtggtt cggccaccac
    18781 aaagcggttc atgtcggctc cttgaaaatg atatgaaaca ttgcatttca tattgcataa
    18841 aggatgccat tctgattaac tcaatatatt caataagtta tgtattgact ggattctgaa
    18901 cggtacaaaa atgatggtca ttaacgttca gcaaaaaatg gattcgacac ccgtgtcgca
    18961 ggggaaagcg agaatgtgat cttgatcacg agaagtgagg tatgctggcc gcaagcgcag
    19021 ccagcacgga atggagcaaa gtaatcaggc gtgttctttc agccagttca gcacaacgtc
    19081 gtggtgattg ctggttttga aatcgtcaaa gacatgttcg attttgccat cagcgtcaat
    19141 caggaagctg atgcgatgaa tgccatcgta ggttttgccc atgaaggact tttcacccca
    19201 gacgccgaat tgttcgcaca cctggtggtc ctcatcagac aggagcgtaa agttaagcag
    19261 ctctttttcc gcaaaacggg agagtttttc gggtttatcg gtgctgatac ccagcacatc
    19321 aacgcccgct tttttcaact catccatgtt atcgcgtaag ccgcaggcct gtacggtaca
    19381 gccgggggtc atggctttcg ggtagaaata aaccagaaca cgctgtccct ggaagtcggt
    19441 caaattaact tgttctccgt cttgatccgg caagctaaat ttcggtgcga tatcaccggc
    19501 tttcagtgga ttcattactt aactccatcc tgttcatcat gttgggaata attgacgacg
    19561 ttaatactgc cttgtgcatt gagttctgta catagggctt tgaacgcttg ctcaatattt
    19621 gctgcgtccg cagatgcggg gctgtgggcg gttatctgaa tatgcaactg cgcagcccgt
    19681 tcattttcag caggttgcgt gcgcgacacc agctccgcaa tgttcatatg atgcgcgtcg
    19741 aaaagtgctg tgaagcgttc aattaaatgc ggggagtctg ccacatcgac ctgaacccag
    19801 acagatgctg gcattggcgg acgcggacgc gccgtcgtgc gcttcatcac gattaaaaga
    19861 tccagttcgg cacctttcaa cggtaacgtt gattcaatca gagtgatggc attccatgaa
    19921 ccggaaagca gcatgataaa cgtgaactct tctcccagca tcgccaggcg actgtcttca
    19981 atattacagc cgcaactact gacatgacgg gtgatggtgt tcacaattcc agggcgatcg
    20041 gcacccaacg cagtgatcac cagataatgt tgcgatgaca gtgtcaaact ggttattcct
    20101 ttaaggggtg agttgttctt aaggaaagca taaaaaaaac atgcatacaa caatcagaac
    20161 ggttctgtct gcttgctttt aatgccatac caaacgtacc attgagacac ttgtttgcac
    20221 agaggatggc ccatgttcac gggaagtatt gtcgcgattg ttactccgat ggatgaaaaa
    20281 ggtaatgtct gtcgggctag cttgaaaaaa ctgattgatt atcatgtcgc cagcggtact
    20341 tcggcgatcg tttctgttgg caccactggc gagtccgcta ccttaaatca tgacgaacat
    20401 gctgatgtgg tgatgatgac gctggagctg gctgatgggc gcattccggt aattgccggg
    20461 accggcgcta acgctactgc ggaagccatt agcctgacgc agcgcttcaa tgacagtggt
    20521 atcgtcggct gcctgacggt aaccccttac tacaatcgtc cgtcgcaaga aggtttgtat
    20581 cagcatttca aagccatcgc tgagcatact gacctgccgc aaattctgta taatgtgccg
    20641 tcccgtactg gctgcgatct gctcccggaa acggtgggcc gtctggcgaa agtaaaaaat
    20701 attatcggaa tcaaagaggc aacagggaac ttaacgcgtg taaaccagat caaagagctg
    20761 gtttcagatg attttgttct gctgagcggc gatgatgcga gcgcgctgga cttcatgcaa
    20821 ttgggcggtc atggggttat ttccgttacg gctaacgtcg cagcgcgtga tatggcccag
    20881 atgtgcaaac tggcagcaga agggcatttt gccgaggcac gcgttattaa tcagcgtctg
    20941 atgccattac acaacaaact atttgtcgaa cccaatccaa tcccggtgaa atgggcatgt
    21001 aaggaactgg gtcttgtggc gaccgatacg ctgcgcctgc caatgacacc aatcaccgac
    21061 agtggtcgtg agacggtcag agcggcgctt aagcatgccg gtttgctgta aagtttaggg
    21121 agatttgatg gcttactctg ttcaaaagtc gcgcctggca aaggttgcgg gtgtttcgct
    21181 tgttttatta ctcgctgcct gtagttctga ctcacgctat aagcgtcagg tcagtggtga
    21241 tgaagcctac ctggaagcgg caccgcttgc ggagcttcat gccccggctg gaatgatttt
    21301 gccggtgacc tccggtgatt atgcaatccc ggtgaccaac ggtagtggtg ctgtcggtaa
    21361 ggcgctggac attcgtccac cagcccagcc gctggcactg gtttctggcg cgcgtaccca
    21421 gttcacgggc gataccgctt cattgctggt ggaaaatggt cgtggcaata ctctgtggcc
    21481 gcaggtggtt agcgtgctgc aggcgaaaaa ctacaccatc acccaacgtg atgatgctgg
    21541 tcagacactg accaccgatt gggtacaatg gaaccgtctg gacgaagacg agcagtatcg
    21601 tggtcgttat caaatctctg ttaagccgca gggttatcag caggcggtta cggttaaact
    21661 gctgaacctg gaacaggcgg gcaaaccggt tgcagacgcg gcttccatgc agcgttacag
    21721 cacggagatg atgaacgtta tttccgccgg tctggataaa tctgccactg acgccgcgaa
    21781 cgctgcgcaa aatcgtgcct ccaccactat ggacgtacaa agtgcagctg atgacaccgg
    21841 tttaccaatg ctggtcgtac gcgggccgtt caatgtggtt tggcaacgtc tgccagcggc
    21901 gctggaaaaa gtgggcatga aagtgaccga cagcacccgt tcgcagggca acatggccgt
    21961 aacttataag ccgctgtctg acagcgactg gcaggaactg ggcgcgagcg atccaggcct
    22021 ggcatccggt gactataaac tgcaggttgg cgatttagat aaccgcagca gcctacagtt
    22081 catcgatccg aaaggtcata ctctgactca gagtcagaac gacgcgctgg tagctgtctt
    22141 ccaggctgcg tttagcaagt aaaaatacag ggctggaatc atccggccct tttttctgat
    22201 atgatacgca aacgtgtgcg tcggcaggaa aacgcgattt tagcggtaat tcgcacgaaa
    22261 tttgtttgtc ggacgtagtt cggataaggc gttcacgccg catccgacaa aacatccggc
    22321 acaccagaca gcaaaagatt ttaaaacgtt aattcacacc caggagtgat aaagatgcaa
    22381 aagcaagctg agttgtatcg tggtaaagcg aaaaccgtat acagcacgga aaacccggac
    22441 ctgttggtgc tcgaattccg caatgatacg tcagcagggg atggcgcgcg cattgagcag
    22501 tttgatcgca aaggtatggt gaacaacaag ttcaactact tcattatgag caaactggct
    22561 gaagcgggta tcccgactca aatggagcgt ctgctctccg ataccgaatg tctggtgaaa
    22621 aagctggata tggtgccggt tgagtgtgtc gtgcgtaacc gtgctgctgg ctctctggtg
    22681 aaacgtcttg gaatcgaaga aggtattgag ctgaacccgc cgctgttcga tctgttcctg
    22741 aaaaacgacg ccatgcacga tccgatggtc aacgaatctt actgcgaaac ctttggctgg
    22801 gtgagcaaag agaacctggc gcgtatgaaa gagctgacct acaaagcgaa cgacgtgctg
    22861 aaaaaactgt tcgatgatgc tggtctgatt ctggtcgact tcaagctgga gtttggtctg
    22921 tacaaaggtg aagtggtact gggcgatgag ttctccccgg acggcagccg cctgtgggac
    22981 aaagaaacac tggagaaaat ggacaaagac cgtttccgcc agagcctcgg tggcctgatc
    23041 gaggcctatg aagccgtcgc ccgccgcctg ggtgtacagc tggactgatt tttctggttg
    23101 cttgtttatt tagccggagg gtactccggc taaatgtctt cgataccccc gctacaatac
    23161 ttcgcattat ctccctttga caggaaagta ataacaggcc aatatgtata tacatgacgt
    23221 atatcccaaa aaaggggggg ctatggaaac tactgtattt ttaagtaatc gcagtcaggc
    23281 cgtcagatta ccgaaagcgg tcgctctgcc agaggatgtt aagaaagtag aaattattgc
    23341 gattggtcgt acaagaatca tcactcccgc cggtgagtcc tgggacagtt ggtttgatgg
    23401 cgaaaatgtg agtgcggatt ttatggatat ccgtgatcaa cccgcaatgc aggaaaggga
    23461 atcattctga tgctgaaatt catgctggat accaatatct gtattttcac tattaagaat
    23521 aaaccagtac atgtcagaga gcggtttaac ctgaattctg gccggatgtg tataagttct
    23581 gtaacattga tggagcttat ctatggggca gaaaaaagtc agatgccaga acgcaatttg
    23641 gcggtgattg aaggtttcgt atctcgtttg gaagtgcttg attatgatag tgctgctgcg
    23701 acgcataccg ggcaaatcag ggccgagctt gcccggcagg gaaggcctgt tggacctttt
    23761 gaccagatga tagcgggaca tgctcgcagt cgcggtttga ttgtggttac caacaatacc
    23821 cgagagtttg agcgagtggc gggaatcaga ctagaagatt ggagctaaca ttctctttta
    23881 cttctaacat tcgtaaatta tataacgtgg catgaagcct taattcctcc gccatttcat
    23941 cccgctttcc tgtggtaatc ttcctctgaa ccacctacga tcatttctat aatgaatata
    24001 ttgttgaggt atctatgcgt tggcaaggac gacgtgaaag tgacaatgtt gaagacaggc
    24061 gcaacagctc tggtggtccg tctatgggcg gtcccggttt tcgcctgcca agcggtaaag
    24121 gcgggctgat tttactgata gtcgtgctgg ttgcaggcta ctatggcgtt gatttaaccg
    24181 ggttgatgac cgggcagccg gtttcccaac agcaatcaac gcggtcaatt agccctaatg
    24241 aagacgaagc cgcaaaattc acctcggtga ttctggcaac cacggaagac acctggggac
    24301 aacagttcga gaagatgggt aagacctatc agcaaccgaa gctggtcatg taccgtggaa
    24361 tgacgcgtac cggatgcggg gcgggccagt ccataatggg gccgttctat tgcccggcgg
    24421 atggcacggt ttatatcgat ctctccttct atgatgacat gaaagacaaa cttggcgcgg
    24481 atggcgattt tgcccagggg tacgttatcg cccatgaagt cggtcatcat gtgcagaaac
    24541 tgttaggcat cgagccgaaa gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtga
    24601 accgcttatc tgtgcgtatg gaactccagg ccgactgttt tgccggtgtc tgggggcata
    24661 gtatgcagca gcaaggcgtt ctggaaaccg gcgatctgga agaggcgctg aacgcggcgc
    24721 aggccatcgg cgatgaccgt ttacaacagc aaagtcaggg gcgagtagta ccagacagtt
    24781 tcactcatgg cacttctcag caacgctaca gctggtttaa acgtggtttc gacagcggcg
    24841 atccggcaca atgcaatact tttggtaaaa gcatttaact tccggggcag ggatggctga
    24901 actgactgcg tttcacacat taacagcgca aatgaaacgt gaagggatcc gccgcttgct
    24961 ggtgttgagc ggggaagagg gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt
    25021 acctggcgac tggctgtgga tttcgccgca gccagatgct gaaaaccact gttctccctc
    25081 ggcactacaa actttacttg ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg
    25141 ctttgatgcc gctgcctttg cggcacttag cggaacgttg aaagcgggaa gctggctggt
    25201 tttgttactc cctgtatggg aagagtggga aaaccaacct gataccgact cgctgcgctg
    25261 gagtgattgc cctgacccta ttgcgacgcc gcattttgtc cagcatttca aacgtgtact
    25321 tacggcggat aacgacgcta ttctctggcg gcaaaaccag ccgttctcgt tggcgcattt
    25381 tactccccgt actgactggc acaccgcgac cggcgcacca caaccagaac aacagcaact
    25441 cttacagcag ctactgacca tgccgtcggg cgtggcagcg gtaacggctg cgcgtgggcg
    25501 cggtaaatcg gcgctggcag ggcaactcat ttctcgtatt gcgggtagtg cgattgtcac
    25561 tgcgcccgca aaagcggcaa cggatgtact ggcacaattt gcgggcgaga agtttcgctt
    25621 tattgcgccg gatgccttgt tagccagcga tgagcaagcc gactggctgg tggtcgatga
    25681 agccgcagcc atacctgcgc cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt
    25741 gttaaccact acggtgcagg gctacgaagg caccggacgt ggttttttgc tgaaattttg
    25801 cgctcgcttt ccgcatttac accgttttga actgcaacag ccgatccgct gggcacaggg
    25861 atgcccgctg gaaaaaatgg tcagcgaggc actggttttt gacgatgaaa acttcaccca
    25921 tacaccacaa ggcaatatcg tcatttccgc atttgaacaa acgttatggc gaagcgatcc
    25981 agaaacgccg ttaaaggttt atcagttatt gtctggtgcg cactaccgga cctcgccgct
    26041 ggatttacgc cggatgatgg atgcaccagg gcaacatttt ttacaggcgg ctggcgaaaa
    26101 cgagattgcc ggggcgttgt ggctggtgga tgagggcgga ttatctcaac aactcagtca
    26161 ggcggtatgg gcaggttttc gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc
    26221 gcacggtagc aatccactgg cagcgacatt gcgtggacgg cgggtcagcc ggatagcagt
    26281 tcatccggcg cgtcagcggg aaggcacagg gcagcaactt attgctggtg ctttgcaata
    26341 tacgcgtgac ctcgactatc tttcggtgag ttttggttac accggggagt tatggcgttt
    26401 ctggcaccgc tgcggttttg tgctggtgcg gatgggtaat catcgtgaag ccagcagcgg
    26461 ttgctatacg gcgatggcgc tgttaccgat gagtaatgcg ggtaaacaac tggctgaacg
    26521 tgagcattac cgtttacgtc gcgatgcgca agctctcgca aagtggaatg gcgaaacgct
    26581 tcctgttgat ccactaaacg atgccgtcct ttctgacgac gactggcttg aactggccgg
    26641 ttttgctttc gctcatcgtc cgctattaac atcgttaggt tgcttattgc gtctgctaca
    26701 aaccagtgaa ctggcattac cggcgctgcg tgggcgttta cagaaaaacg tcagcgacgc
    26761 gcagttatgt accacactta aactttcagg ccgcaagatg ttactggtcc gtcagcggga
    26821 agaggccgcg caggcgctgt tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg
    26881 cataacgcaa tggcaatttt ttcactgact ccttcaattt tctcccatgg tcattgtgag
    26941 tcagcggcgt aaaattaatg acatcagtta aggagttcgc catgaaacat gaccattttg
    27001 ttgttcaaag cccggataaa cctgcgcaac agttgttact gcttttccac ggtgtcgggg
    27061 ataacccggt ggcaatgggg gaaataggta gctggtttgc accgctgttt cctgatgcgc
    27121 tggtggtgag tgtcggtggc gcggagccga gcggtaatcc ggcggggcgt cagtggtttt
    27181 cggtgcaggg tattacggaa gataatcgcc aggcgcgtgt ggatgcgatc atgccgacgt
    27241 ttattgaaac ggtgcgctac tggcagaaac agagcggagt aggggctaac gccacggcgc
    27301 tcatcggttt ctctcaaggc gcgattatgg tgctggagag cattaaagcc gaaccgggcc
    27361 ttgcttcacg cgtcattgct tttaacggac gctatgcgag cctgccggaa actgcgtcta
    27421 cagccacgac aattcacctg attcatggcg gtgaagatcc ggttatcgat ctggcgcatg
    27481 cggttgcggc gcaggaagcg ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg
    27541 aggatttagg ccatgcgatc gacaatcgca gtatgcaatt tgccctggat cacttgcgtt
    27601 ataccattcc gaaacattat ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg
    27661 atgtgattga aatgatgtaa taatagccgg gtaggcctga taagactgta tcaggcatca
    27721 tggccggatg tggcgtgaac gccttatccg gccactcagc ttacttcttc ggttgatcct
    27781 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
    27841 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
    27901 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
    27961 tgcttatttc ctcttgcaga accactcatc acgcgacgag ctgttccatg atacgttgat
    28021 acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag
    28081 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgggcaata aagcgcccgt
    28141 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
    28201 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
    28261 agtccaccgt ataacgcagt tgatgttttt caagcagggc aagcacctgc gctttgatca
    28321 tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa
    28381 taacgttgtt actgcccgtt cccgcctgaa tattagcaat ctgcatactg gtcgccggga
    28441 aaaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
    28501 gatgtactgg attgtcagcc agatgcgggt aggccacatg cccctgaacg ccatgaatgg
    28561 taaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacca accacttcga
    28621 tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgcgac attaacgctt
    28681 cgacgacttt taccgtaccg ttgtgggcac tagcttcttc atcagaagtg atcagaaatg
    28741 ccagtcgccc cgtatggttg ggatgttgtg cgacaaaacg ttctgccgct accaccatcg
    28801 ccgccagcga gcctttcata tctgccgcac cgcgcccgaa taacatgccg tcacgaatgg
    28861 ttggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
    28921 gcccggcaaa ggctaacgtt tcaccctgcc cacgccatgc ccaaaaattc tgcgtatcgg
    28981 caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
    29041 ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
    29101 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
    29161 aacccagcag cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca
    29221 tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt
    29281 ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct aattcgttga
    29341 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
    29401 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
    29461 cgtaaagtgt
//
LOCUS       DAPPNJ010000039        29194 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000039 DAPPNJ010000000
VERSION     DAPPNJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.039"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            59..727
                     /gene="pspA"
                     /locus_tag="RPM73_001376"
     CDS             59..727
                     /gene="pspA"
                     /locus_tag="RPM73_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDX6519750.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            781..1005
                     /gene="pspB"
                     /locus_tag="RPM73_001377"
     CDS             781..1005
                     /gene="pspB"
                     /locus_tag="RPM73_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDX6519751.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            1005..1364
                     /gene="pspC"
                     /locus_tag="RPM73_001378"
     CDS             1005..1364
                     /gene="pspC"
                     /locus_tag="RPM73_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDX6519752.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            1373..1594
                     /gene="pspD"
                     /locus_tag="RPM73_001379"
     CDS             1373..1594
                     /gene="pspD"
                     /locus_tag="RPM73_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDX6519753.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            1669..1983
                     /gene="pspE"
                     /locus_tag="RPM73_001380"
     CDS             1669..1983
                     /gene="pspE"
                     /locus_tag="RPM73_001380"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDX6519754.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            2195..3874
                     /gene="ycjM"
                     /locus_tag="RPM73_001381"
     CDS             2195..3874
                     /gene="ycjM"
                     /locus_tag="RPM73_001381"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDX6519755.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            3888..5180
                     /locus_tag="RPM73_001382"
     CDS             3888..5180
                     /locus_tag="RPM73_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6519756.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            5201..6082
                     /locus_tag="RPM73_001383"
     CDS             5201..6082
                     /locus_tag="RPM73_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDX6519757.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            6069..6911
                     /locus_tag="RPM73_001384"
     CDS             6069..6911
                     /locus_tag="RPM73_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDX6519758.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            6942..7994
                     /locus_tag="RPM73_001385"
     CDS             6942..7994
                     /locus_tag="RPM73_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDX6519759.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            8013..8801
                     /locus_tag="RPM73_001386"
     CDS             8013..8801
                     /locus_tag="RPM73_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDX6519760.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTTCGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            8811..9866
                     /locus_tag="RPM73_001387"
     CDS             8811..9866
                     /locus_tag="RPM73_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX6519761.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            9863..12130
                     /gene="ycjT"
                     /locus_tag="RPM73_001388"
     CDS             9863..12130
                     /gene="ycjT"
                     /locus_tag="RPM73_001388"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDX6519762.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            12127..12786
                     /gene="pgmB"
                     /locus_tag="RPM73_001389"
     CDS             12127..12786
                     /gene="pgmB"
                     /locus_tag="RPM73_001389"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDX6519763.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            12800..13882
                     /locus_tag="RPM73_001390"
     CDS             12800..13882
                     /locus_tag="RPM73_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6519764.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            13927..14832
                     /gene="ompG"
                     /locus_tag="RPM73_001391"
     CDS             13927..14832
                     /gene="ompG"
                     /locus_tag="RPM73_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDX6519765.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(14943..15941)
                     /locus_tag="RPM73_001392"
     CDS             complement(14943..15941)
                     /locus_tag="RPM73_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX6519766.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            16096..17493
                     /locus_tag="RPM73_001393"
     CDS             16096..17493
                     /locus_tag="RPM73_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDX6519767.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            17490..18551
                     /locus_tag="RPM73_001394"
     CDS             17490..18551
                     /locus_tag="RPM73_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDX6519768.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            18699..20240
                     /gene="tyrR"
                     /locus_tag="RPM73_001395"
     CDS             18699..20240
                     /gene="tyrR"
                     /locus_tag="RPM73_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDX6519769.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(20284..20790)
                     /gene="tpx"
                     /locus_tag="RPM73_001396"
     CDS             complement(20284..20790)
                     /gene="tpx"
                     /locus_tag="RPM73_001396"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDX6519770.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            20909..21874
                     /gene="ycjG"
                     /locus_tag="RPM73_001397"
     CDS             20909..21874
                     /gene="ycjG"
                     /locus_tag="RPM73_001397"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDX6519771.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASALWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(21849..22577)
                     /gene="mpaA"
                     /locus_tag="RPM73_001398"
     CDS             complement(21849..22577)
                     /gene="mpaA"
                     /locus_tag="RPM73_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDX6519772.1"
                     /translation="MTVTRPRAERGVFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(22868..23506)
                     /locus_tag="RPM73_001399"
     CDS             complement(22868..23506)
                     /locus_tag="RPM73_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6519773.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(23524..24456)
                     /locus_tag="RPM73_001400"
     CDS             complement(23524..24456)
                     /locus_tag="RPM73_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX6519774.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVKVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(24456..25376)
                     /locus_tag="RPM73_001401"
     CDS             complement(24456..25376)
                     /locus_tag="RPM73_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX6519775.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            25514..26413
                     /gene="pgrR"
                     /locus_tag="RPM73_001402"
     CDS             25514..26413
                     /gene="pgrR"
                     /locus_tag="RPM73_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDX6519776.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            26750..28363
                     /gene="mppA"
                     /locus_tag="RPM73_001403"
     CDS             26750..28363
                     /gene="mppA"
                     /locus_tag="RPM73_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDX6519777.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(28414..>29194)
                     /gene="ynaI"
                     /locus_tag="RPM73_001404"
     CDS             complement(28414..>29194)
                     /gene="ynaI"
                     /locus_tag="RPM73_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDX6519778.1"
                     /translation="LIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIV
                     VLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIR
                     SPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLR
                     YEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEW
                     LAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         7258 a         6961 c         7700 g         7275 t
ORIGIN      
        1 aattgtatta acagttcagc aggacaatcc tgaacgcaga aatcaagagg acaacattat
       61 gggtattttt tctcgctttg ccgacatcgt gaatgccaac atcaacgctc tgttagagaa
      121 agcggaagat ccacagaaac tggttcgtct gatgatccag gagatggaag atacactggt
      181 tgaagtacgt tctacttcgg cgcgtgcgtt ggcagaaaag aaacagctga ctcgccgtat
      241 tgaacaagcg tcggcgcgtg aagttgaatg gcaggaaaaa gccgaactgg cgctgctgaa
      301 agagagagag gatttggcac gtgcagcgtt aattgaaaaa cagaaactga ccgatctgat
      361 taagagcctg gaacatgaag tgacgctggt ggacgacacg ctggcacgca tgaagaaaga
      421 gatcggtgag ctggaaaaca aattgagcga aacacgcgct cgccagcagg cattgatgtt
      481 acgtcatcag gcggcaaact cgtcgcgcga tgtgcgtcgt cagctggaca gtggcaaact
      541 ggatgaagca atggctcgtt tcgaatcttt cgaacgtcgt attgaccaga tggaagcgga
      601 agcagaaagc cacagcttcg gtaaacaaaa atcgctggac gatcagtttg ccgaactgaa
      661 agccgatgat gcaatcagcg aacaactggc acaattaaaa gccaaaatga agcaagacaa
      721 tcaataataa catccaggcg gcgtccgaac gcgccgccgc tcatcgtcta aggagtactt
      781 atgagcgcgc tatttctggc tattccgtta accatttttg tgctgtttgt tttaccgatc
      841 tggttatggc tgcattacag caatcgttct ggtcgcagtg aattgtcgca aagtgagcag
      901 cagcgattag cgcaactggc tgatgaagca aaacggatgc gcgaacgtat tcaggcgctg
      961 gaatctattc ttgatgcaga acatccgaac tggagggatc gctaatggcg ggcattaatc
     1021 tcaataaaaa attatggcgt attccacagc agggcatggt ccgcggcgtc tgcgccggga
     1081 ttgccaacta ttttgatgta ccggtaaaac tggtgcgtat cctggtggtg ctgtcgattt
     1141 tcttcggtct ggcgctgttt accctggttg cttacatcat tttgtcattt gcgcttgatc
     1201 caatgccgga caacatggcc tttggtgagc agctaccttc cagcagcgaa ttgctggatg
     1261 aagtcgaccg tgaactggcg gcaagtgaaa cgcgtttacg cgagatggaa cgttatgtca
     1321 cttccgatac tttcacgtta cgtagccgtt tccgtcaact gtgaggaaag ttatgaatac
     1381 tcgctggcaa caggccgggc aaaaggtaaa gcctggtttc aaattagcag gcaagctggt
     1441 acttcttacc gcactgcgct atggcccggc gggtgtggcg ggctgggcga taaaatcagt
     1501 tgctcgccga ccgctgaaaa tgttgctggc tgtggcgctg gaaccgctgt taagtcgggc
     1561 tgctaataaa ctggcacagc gttataaaag gtgaggggag tttcgcaaaa attgttaaat
     1621 ctcaggcgta taatggatgg caattttcat ccatagaagg acgcttacat gtttaaaaaa
     1681 ggcttacttg ctctggcact ggtgttttca ctgcccgttt tcgccgctga acactggatc
     1741 gatgttcgtg ttccagagca gtatcagcaa gagcacgttc agggggccat caatattccc
     1801 ctgaaggaag tgaaagagcg cattgccacc gccgttccgg ataaaaacga caccgtgaaa
     1861 gtgtattgca atgccggacg ccagtcaggg caagcaaaag agatccttag cgagatggga
     1921 tatacccacg ttgagaacgc cggtggcctg aaagacatcg caatgccgaa ggtcaaaggt
     1981 taaaagaatt caccatgagc ggcgcttatg cgccgttttt tttctgtcac accttattta
     2041 catccccata gattatttgc gtcagctcac aaatacgctt tttccctggt aaaaaatgat
     2101 ttcctgcgtg actaaaaccc ttgtgctcaa ttgacagttt attttctgcg gagtagtctc
     2161 tcgtttcatg ggaccgctac cacggaaagg caacatgaaa cagaaaatta cggattacct
     2221 ggacgaaatc tacggtggaa catttaccgc aactcattta cagaaacttg taacgcgtct
     2281 tgagagtgcg aaacgattaa ttacacagcg acgtaaaaaa cactgggatg aaagtgatgt
     2341 cgtgttaatt acctatgccg atcaatttca cagcaatgat ttaaaaccat tacccacatt
     2401 taatcagttt taccatcaat ggctgcaaag cattttttca catgttcatt tgttgccgtt
     2461 ttatccatgg tcatctgatg atggcttttc ggtaattgat tatcaacagg tcgccagtga
     2521 agcgggggag tggcaggata ttcagcaact cggtgaatgt agtcatttaa tgtttgattt
     2581 tgtctgcaac catatgtcgg caaaaagtga atggtttaaa aactatttac aacagcatcc
     2641 aggttttgaa gattttttta ttgccgttga cccgcaaacc gatctcagcg ccgtcactcg
     2701 cccgcgtgcg ttaccgttat taacgccatt ccagatgcgc gatcattcaa cgcgccattt
     2761 atggaccacc tttagtgacg atcaaattga cctgaattac cgtagccctg aagtgttgct
     2821 ggcgatggtg gatgttttac tctgttacct tgcgaaaggt gctgagtatg tccgcctgga
     2881 tgccgttggc tttatgtgga aagagccggg aacaagctgc atccatctgg aaaaaacaca
     2941 tctgattatc aaactgttac ggtcgattat tgataacgtt gcgccaggta cagtgatcat
     3001 taccgagacc aatgttccgc ataaagacaa cattgcttac tttggcgcag gcgatgacga
     3061 agcacatatg gtgtaccagt tctcgctgcc gccactggtg ctgcatgcgg tgcaaaaaca
     3121 gaacgttgag gcgctttgtg cgtgggcgca aaacctgaca ctaccttcca gcaacaccac
     3181 ctggtttaac ttcctcgcct ctcacgatgg catcgggcta aacccgctac ggggcttgtt
     3241 gcctgaaagc gaaatattag agctggtcga ggcgttacag caggaaggtg cattagtaaa
     3301 ctggaaaaat aatcccgacg gtacacgcag tccgtatgaa ataaatgtga cctatatgga
     3361 tgcgttaagc cgccgtgaga gtagcgatga agaacgttgc gccaggttta ttctcgccca
     3421 tgcgattttg ttaagtttcc ccggtgtgcc agcgatatat attcaaagta ttcttggctc
     3481 gcgtaatgat tacgcaggtg tcgaaaaact cggatataac cgtgcgatta accgtaaaaa
     3541 atatcacagt aaagagataa cccgagaact gaacgatgaa gctacattaa ggcatgcggt
     3601 atatcatgag ttgtcgcgtt taattacact tcgtcgcagc cataacgagt ttcatccgga
     3661 taataatttt accattgata cgattaattc atccgtaatg cgtattcaaa gaagtaacgc
     3721 tgatggtaat tgtctgactg gattgtttaa tgtcagtaaa aatattcagc atgtaaatat
     3781 tactaatctg catggtcggg atctgattag tgaagttgat atattgggta atgaaataac
     3841 gctgcgcccc tggcaggtta tgtggattaa ataaaaagga acatctcatg attaaatcaa
     3901 aaatcgtgct gttatcagca ctggtttcct gcgccctgat ttcaggctgt aaagaagaaa
     3961 ataaaacgaa tgtatccatc gaatttatgc attcttcggt ggagcaggag cgccaggccg
     4021 ttatcagtaa attgattgcc cgttttgaaa aagaaaaccc tggcatcacc gttaagcaag
     4081 tgcccgtgga agaagatgcc tataacacta aagtcattac tctttcacgt agcggttcgc
     4141 tgccggaagt gatcgaaacc agccatgact acgccaaagt gatggacaaa gagcagctta
     4201 tcgatcgcaa agcggttgcc acagtcatca gcaacgttgg tgaaggcgcg ttttacgatg
     4261 gcgtactgcg tattgtgcgt accgaagatg gtagcgcatg gaccggtgtt cctgtcagcg
     4321 cctggattgg cggtatctgg tatcgcaaag atgtgctggc aaaagcgggg cttgaggagc
     4381 cgaaaaactg gcaacagctg ctggacgttg cacagaaact gaatgacccg gcgaataaaa
     4441 aatacggcat tgcgctgcct acagcagaaa gcgtgttgac ggaacaatcc ttctcccagt
     4501 ttgcgttatc caaccaggct aacgtcttta acgccgaagg caaaatcacc cttgatacac
     4561 cagagatgat gcaggcactg acctattacc gcgaccttgc tgccaacact atgccgggtt
     4621 ctaacgacat catggaagtg aaagacgcct ttatgaacgg caccgcgccg atggcgattt
     4681 actccaccta tatccttccg gctgtgatta aagaaggcga cccgaaaaac gtcggtttcg
     4741 tggtgccaac cgagaaaaac tctgcggtct acggcatgtt gacctcgctg accattaccg
     4801 ccgggcaaaa gaccgaagag acggaagcag cagaaaaatt tgtcaccttt atggagcagg
     4861 cagacaacat tgccgactgg gtgatgatgt cgccaggtgc tgcgctgccg gtgaataaag
     4921 cggttgtcac taccgccacc tggaaagaca acgacgttat taaggcgctg ggtgaactac
     4981 cgaatcagct aatcggtgaa ctgccaaata ttcaggtctt tggtgcagta ggggataaaa
     5041 actttacccg catgggtgat gtgacggggt ctggagtggt gagctccatg gtgcataacg
     5101 tcaccgtggg taaagccgat ctccctggca cgctgcaagc gagccagaaa aagctggatg
     5161 aactgatcga acagcactaa accccggaca ggaatccgca atgaacaggc ttttttcagg
     5221 tcgttccgat atgccctttg cgctgttgct tctcgcgccc agcttattac tgctgggcgg
     5281 tctggtggcg tggccgatgg tgtcgaatat cgaaatcagt tttttacgtc tgccgctcaa
     5341 tcccaacatc gagtcaacct ttgttggggt gagtaactat gtgcgtatcc tctccgatcc
     5401 cggcttctgg cactcgctgt ggatgacggt ctggtatacc gcgctggtgg tggcgggcag
     5461 caccgtgctc gggctggcag tggcgatgtt tttcaaccgc gagttccgtc tgcgcaaaac
     5521 cgcgcgttcg ctggtgatcc tctcctacgt aacgccgtcc atttcgctgg tgtttgcctg
     5581 gaaatacatg ttcaacaacg gctacggcat tgtgaactac ctcggcgtcg atcttctgca
     5641 tctctatgag caggcaccgc tgtggttcga taatccgggc agtagctttg tgctggtggt
     5701 gctgttcgcc atctggcgct acttcccgta tgcctttatc tcgtttctgg cgattttgca
     5761 gaccatcgac aaatcgctgt acgaagcggc agagatggat ggcgctaatg cctggcaacg
     5821 gtttcgtatc gtcacgctgc ccgcgattat gccggtcctg gcgacggtgg tgaccctgcg
     5881 caccatctgg atgttctaca tgttcgcgga tgtttatttg ctgacgacca aggtcgatat
     5941 tctcggtgta tatctctaca aaaccgcctt tgcctttaat gatttaggaa aagcggcggc
     6001 gatctcggtg gtgctcttca tcattatttt cgctgtcatt ctgctgacca ggaaacgggt
     6061 gaacctcaat ggcaacaaat aaacgcacac tcagtcgcat cggtttttac tgcgggctgg
     6121 cgctgtttct catcatcacg ctgtttccat tttttgtgat gctgatgacc tcgttcaagg
     6181 gcgcgaaaga ggcgatctca ctgcatccta cgctgctgcc gcagcagtgg acgctggagc
     6241 attacgtcga catttttaac ccgatgattt tcccgtttgt cgactacttc cgtaacagtc
     6301 tggtggtgtc ggtggtttca tccgtggtgg cggtattcct cggcattctt ggggcttacg
     6361 cgctttcccg cctgcgcttt aaaggtcgga tgaccatcaa tgccagcttt tacacggtgt
     6421 atatgttctc tggcattttg ctggtggtgc cgctgttcaa aatcatcacc gcgctgggca
     6481 tttacgacac cgagatggcg ctgatcatca ccatggtgac gcaaactctg cccaccgccg
     6541 tgtttatgct gaaaagctac ttcgacacca tccccgatga gatcgaagaa gcggcgatga
     6601 tggatggcct caaccgtctg caaattatct tccgcatcac cgtgccgttg gcgatgtccg
     6661 ggctgatatc cgtgttcgtc tactgcttta tggtggcgtg gaacgactat ctgtttgcgt
     6721 cgattttcct ctccagcgcc agcaatttca ccttaccggt gggcctgaac gcgctgttca
     6781 gtacgccaga ttacatctgg ggacggatga tggcggcctc actggtgacc gcattgccgg
     6841 tggtgattat gtatgcgctt tcggaacgtt ttattaagag tggtttgacc gccggtggcg
     6901 tgaagggcta aagcggccag tttctttaca aggagtttta aatgaaaaag ttagtagcca
     6961 cagcaccgcg tgttgctgcg ctggttgagt atgaagatcg ggcgatttta gccaatgaag
     7021 tgaaaatccg cgtgcgtttc ggcgcaccga aacacggaac ggaagtggtc gacttccgcg
     7081 ccgccagccc gtttatcgat gaagacttta acggtgaatg gcagatgttc actccgcgtc
     7141 cggcagatgc gccgcgcggc attgagtttg gcaaattcca gcttggcaac atggtggttg
     7201 gcgacattat cgagtgcggc agcgacgtaa ccgactacgc ggtgggcgac agcgtatgcg
     7261 gctacggccc actctccgag acggtcatca ttaacgcggt gaataactac aagctgcgca
     7321 aaatgccgca aggcagctcc tggaaaaacg ccgtctgtta cgacccggcg cagtttgcca
     7381 tgagcggcgt gcgcgatgcc aacgtgcgcg tgggtgattt tgtggtggtg gtagggcttg
     7441 gcgcgattgg tcaaatagcc atccaactgg cgaaacgcgc tggcgcatcg gtggtaattg
     7501 gcgtcgatcc tatcgcccat cgctgtgata ttgcccgtcg ccacggtgcg gatttctgcc
     7561 ttaaccccat cggcactgac gtaggtaaag agatcaaaac gctgaccggc aagcagggtg
     7621 ccgatgtgat tatcgaaacc agcggttacg ccgacgcgct gcaatcggcg ctccgcggcc
     7681 tggcttatgg cggcaccatc tcgtatgttg cgtttgccaa accgtttgcc gaaggtttta
     7741 acctcggacg cgaagcccat ttcaataacg ccaaaattgt cttctcccgc gcgtgcagcg
     7801 aaccgaaccc ggattatccg cgctggagcc gcaagcgtat cgaagaaacc tgttgggaac
     7861 tgctgatgaa cggttatctc aattgtgaag atctaatcga cccggtggtg acctttgcca
     7921 atagcccgga aagctacatg cagtatgtcg accagcatcc ggaacagagc atcaaaatgg
     7981 gcgttacgtt ttaattcaaa ggaatgaaaa aaatgaaaat aggcacacag aatcaggcgt
     8041 tctttccgga aaatattctg gagaaatttc gttatatcaa agagatgggt ttcgatggtt
     8101 ttgagatcga cggcaaactg ctggtcaaca atctcgaaga agtcaaagcg gcgatcaaag
     8161 aaactggttt accggtgacc accacctgcg gtggctatga cggctggatt ggtgacttta
     8221 tcgaagagcg tcgtcttaat ggcttaaagc agatcgaacg cattctcgaa gcgctggcag
     8281 aagtgggggg taaaggcatc gtcgttccgg cagcgtgggg catgtttacc ttccgcttac
     8341 cgccgatgac ctcgccgcgt agcctggacg gcgaccgcaa aatggtgagt gattccctgc
     8401 gcgtactgga acaggtcgcc gcgcgtaccg gaaccgtggt gtatctcgaa ccgttaaacc
     8461 gctatcagga tcatatgatc aacaccctcg ccgatgctcg tcgttacatc gtcgaaaaca
     8521 atcttaaaca tgtgcagatt atcggcgatt tctatcacat gaacatcgaa gaagataacc
     8581 tggcgcaggc gctgcacgac aaccgtgacc tgctcggtca tgtgcatatt gccgataacc
     8641 atcgttacca gccgggcagt ggcaccctcg atttccacgc gctgtttgaa cagctgcgcg
     8701 ccgataacta tcagggttat gtggtgtatg aagggcgtat ccgggcagaa gatcctgccc
     8761 aggcgtaccg tgattcgttg gcctggttgc gtacctgcta agaggtcttt gtgaaaagtg
     8821 caatgacaag ctctccgctg cgggtcgcga taataggcgc aggccaggtg gcggataagg
     8881 ttcatgcttc gtactactgc acccgcaacg atctggaact ggtggctgtc tgtgacagcc
     8941 gcctttccca ggcgcaggcg ctggcagaaa aatacgggaa tgcatccgtg tgggacgatc
     9001 cgcaggccat gctgctggcg gtgaaacctg atgtggttag cgtctgctca cctaaccgtt
     9061 ttcattacga acataccctg atggcactgg aagcgggctg ccatgtgatg tgcgaaaaac
     9121 cgcccgccat gacgccagaa caggcgcggg aaatgtgcga taccgcgcgc aaactgggca
     9181 aggtgctggc ctacgacttt caccatcgtt ttgcgctcga tacgcaacag ctgcgtgacc
     9241 aggtgaccaa cggcgttttg ggagagattt acgttaccac cgcccgcgcc ctgcgtcgct
     9301 gcggcgttcc tggctggggc gtctttacca ataaagaact gcagggtggt ggcccgctga
     9361 tcgacatcgg cattcatatg ctggatgctg cgatgtatgt gctgggcttt ccggcggtga
     9421 aaagcgtgaa tgcgcatagc tttcaaaaga tcggcacgca aaagagctgt ggtcaatttg
     9481 gcgagtggga tccggcaact tatagcgtcg aagattcgct gtttggcacc attgaatttc
     9541 ataacggcgg cattctgtgg ctggaaacgt catttgcact caacatccgc gaacagtcga
     9601 ttatgaacgt cagcttttgt ggtgataaag ccggtgcgac gctgtttcca gcacatatct
     9661 acaccgataa caacggtgaa ttaatgacgc tgatgcaacg ggaactggca gacgacaacc
     9721 gccatttgcg cagcatggaa gcctttatca atcacgtaca gggcaagccc gtgatgatag
     9781 ccgacgccga gcaggggtac atcatccagc aactggtggc ggcgttgtat caatccgcag
     9841 aaacagggac gcgtgtggaa ttatgaccag gccagtaacg ttatcagaac cccatttcag
     9901 ccagcatacc ctgaacaagt atgcatcgct gatggcgcag gggaacggct atcttgggct
     9961 tcgcgccagc catgaagaag attacacgcg ccagacgcga gggatgtatc tggcggggct
    10021 gtatcatcgg gcgggaaaag gtgaaatcaa cgaactggtg aacctgcctg atatcttggg
    10081 gatggagatt gccataaatg gtgaggtttt ctcgttatcc cgcgaagcct ggcagcgtga
    10141 acttgacttt gccagtggag aattacgccg tagcgttgtc tggcgtacca gcaacggcac
    10201 aggttacacc atcgccagcc gtcgctttgt ttcggcagac caactgccgc tcattgtgct
    10261 ggaaatcact attacgccac tggacgccga cgcgtcagtg ctgatttcaa caggtatcga
    10321 cgccacgcaa accaaccacg gtcgccaaca tctcgacgaa acccaggtgc gggtgtttgg
    10381 tcagcatctg atgcagggga tctacaccac ccaggatgga cgcagtgatg tggccatcag
    10441 ctgttgctgt atggtgagcg gtgatgtgca gcaatgctat accgccaaag agcgccgttt
    10501 gcagcaacat accagtgcgc agcttcatgc aggcgagaca gtgacgttgc aaaaactggt
    10561 gtggatcgac tggcgggatg acaggcaagc cgttttagac gagtggggca gcgcgtcgct
    10621 tcgccagctt gaaatgtgcg cgcagcagag ttacgaccaa cttcttgcag tatcaacaga
    10681 aaactggcgt caatggtggc agaaacgtcg tatcacggta aatggcggcg aagcgcacga
    10741 tcagcaagcg ttagattatg cgctttatca tctgcgcatc atgacgcctg cccacgacga
    10801 gcgcagcagc attgcggcaa aaggcttaac cggcgaaggc tacaaaggcc acgttttctg
    10861 ggatacagaa gtatttttgt taccgtttca tctgtttagc gatccgacgg ttgcccgaag
    10921 tttactgcgt tatcgctggc acaacttgcc aggcgcgcag gagaaagcgc gacgcaacgg
    10981 ctggcagggc gcgctatttc cgtgggaaag cgcgcgcagc ggcgaagaag agacgccgga
    11041 atttgccgcc attaacattc gcaccgggct gcggcaaaaa gtggcctcgg cgcaggcgga
    11101 acatcatctg gtggccgata tcgcctgggc ggttattcaa tactggcaga ccacggggga
    11161 tgaaagtttc attgcgcatg aaggcatggc gctacttctg gaaactgcaa agttctggat
    11221 tagccgcgcg gtgagggtta acgaccgtct ggaaattcat gatgttattg ggcctgacga
    11281 atataccgaa catgtcaata ataacgcctt caccagctat atggcgtatt acaacgtcca
    11341 gcaggcgctg agtattgccc gccagtttgg ctgtagcgac gatgcgttta tccatcgcgc
    11401 cgaaatgttc cttaaagaac tgcggctgcc agaaattcag cccgacggcg ttttgccgca
    11461 ggatgattcg tttatggcta agccggcgat taatctggcg aaatacaaag cggcggcggg
    11521 gaagcaaacc attctgctgg attattcacg cgcagaagtg aacgagatgc aaatcctcaa
    11581 acaagctgat gtggtgatgc tcaattacat gctgccggag cagttctcag cggcatcgtg
    11641 tcttgccaat ctgcaatttt atgaaccgcg cactattcac gactcgtcat taagtaaagc
    11701 aatccacggc attgttgccg cacgctgtgg cctgctgacc caaagttatc agttctggcg
    11761 cgaggggact gaaatcgatc ttggtgctga tccgcatagt tgtgatgatg gtatccatgc
    11821 tgccgcaact ggcgctatct ggctgggggc gattcagggt tttgccgggg tgagcgtgcg
    11881 tgacggtgaa ttgcatctca atccggcgtt acctgagcag tggcaacagt tgtctttccc
    11941 tctgttctgg cagggctgcg aattacaggt cactcttgac gcgcagcgta ttgcgattcg
    12001 aacttctgcg cccgtttcac tgcgtttgaa cggtcagctt atatccgtgg ctgaagaatc
    12061 tgttttctgt ttgggtgatt ttattttgcc cttcaatggg accgctacca cacatcagga
    12121 ggatgaatga aactgcaagg ggtaattttc gatctggatg gtgtaatcac cgataccgcg
    12181 catctgcatt tccaggcgtg gcagcagatt gccgctgaaa ttggcatcag cattgatgcg
    12241 cagtttaacg aatccctaaa agggatcagc cgcgatgagt ctctgcggcg cattctgcaa
    12301 cacgggggca aagagggcga ctttaactcg caggagaggg cgcaactggc gtatcgcaaa
    12361 aatctgctct atgtccactc actgcgcgag ttgacggtca gcgctgttct acccggcatt
    12421 cgctctttgc tggcagatct ccgtgcacag cagatcccgg ttgggctggc ttctgtctcc
    12481 ctgaatgcgc cgacgatttt agcggcgctg gagctgcgcg agtttttcac cttctgcgcg
    12541 gatgcttccc aacttaaaaa ctcgaaaccg gacccggaaa tctttctcgc cgcctgtgcc
    12601 gggctgggcg tgccgccgca ggcatgtatc ggcattgaag atgcgcaggc gggcattgac
    12661 gccattaacg ccagcggtat gcgctcggtg gggatcggcg cgggcttaac cggggcgcaa
    12721 ttactgttgc cttcaacgga ctctctgacc tggtcgcggt tatcggcctt ctggcaaaac
    12781 gtatagcaaa ggaatcaaca tggctcagct ttcgttacaa catattcaaa aaatctacga
    12841 taaccaggtg catgtggtga aggacttcaa cctggaaatt gccgataaag agttcatcgt
    12901 gtttgtcggc ccgtcgggct gcggtaagtc gaccaccctg cgcatgattg ccgggcttga
    12961 ggagatcagc ggcggcgatc tgttgatcga cggcaaacga atgaatgacg ttccagccaa
    13021 agcacgcaat atagcgatgg tgttccagaa ctacgcgttg tatccgcata tgacggttta
    13081 cgacaacatg gcgtttggcc tgaagatgca aaaaatcgcc aaagaggtga ttgatgagcg
    13141 ggtgaactgg gcggcgcaaa ttctcggcct gcgtgagtac ctgaaacgca agccgggcgc
    13201 gctttccggc gggcaacgcc agcgtgtggc gctcgggcgg gcgattgtgc gcgaagcggg
    13261 cgtgttttta atggatgaac cgctctctaa ccttgatgcc aagctgcgcg tgcaaatgcg
    13321 cgcagagatc agcaagctac accagaagct gaataccacc atgatctacg tgacccacga
    13381 tcagaccgaa gcgatgacca tggcgacgcg gattgtgatt atgaaggatg ggattgttca
    13441 gcaagttggt gcgccgaaaa cggtttataa ccaaccagcg aatatgtttg ttgccggatt
    13501 tattggatca ccggcgatga attttattcg tggcacgatc gatggcgata aattcgttac
    13561 ggaaacgctt aaattaacca ttcccgaaga gaaattagcg gttctgaaaa cacagggaag
    13621 tttgcataag cccatcgtga tgggaatacg tccggaagat attcatccgg acgcgcaaga
    13681 ggaaaataac atttccgcca aaattagcgt ggcagaatta accggtgcgg aatttatgct
    13741 ctacaccacg gttggggggc acgagttagt ggtccgtgct ggtgcgttaa atgattatca
    13801 tgcaggagaa aatatcacta ttcattttga tatgacgaaa tgtcatttct ttgatgcaga
    13861 aacggaaata gcaattcgct aaatacaggg ggaaggcatt cccccagaat aatacaagga
    13921 acaataatga aaaagttatt accctgtacc gcactggtga tgtgtgcggg aatggcctgc
    13981 gcacaggccg aggaaaggaa cgactggcac tttaatatcg gcgcgatgta cgaaatagaa
    14041 aacgtcgagg gttatggcga agatatggat gggctggcgg agccttcagt ctattttaat
    14101 gccgccaacg ggccgtggag aattgctctg gcctattatc aggaagggcc ggtagattat
    14161 agcgcgggta aacgtggaac gtggtttgat cgcccggagc tggaggtgca ttatcagttc
    14221 ctcgaaaacg atgatttcag tttcggcctg accggcggtt tccgtaatta tggttatcac
    14281 tacgttgatg aaccgggtaa agacacggcg aatatgcagc gctggaaaat cgcgccagac
    14341 tgggatgtga aactgactga cgatttacgt ttcaacggtt ggttgtcgat gtataaattt
    14401 gccaacgatc tgaacacgac cggttacgct gatacccgag tcgaaacgga aacaggcctg
    14461 caatatacct tcaacgaaac ggttgccttg cgagtgaact attatctcga gcgcggcttt
    14521 aatatggacg acagccgcaa taacggtgag ttttctacgc aagaaattcg cgcctatttg
    14581 ccgctgacgc tcggcaacca ctcggtgacg ccgtatacgc gcattgggct ggatcgctgg
    14641 agtaactggg actggcagga tgatattgaa cgtgaaggcc atgattttaa ccgtgtaggt
    14701 ttattttacg gttatgattt ccagaacggg ctttccgttt cgctggaata cgcgtttgag
    14761 tggcaggatc acgacgaagg cgacagcgat aaattccatt atgcaggtgt cggcgtaaat
    14821 tactcgttct gataatgggc taaattgccg gatgcggcac gagtacttta tccgaactat
    14881 aaatgtaggc cggataagat gcgctagcat cgcatccggc attcaggcaa ggtagctggt
    14941 atttatttca gcgtcatatg cgtggcaacg gtaatattct gtggtgacgg ttttccagaa
    15001 attaagcgga ataataactc acagctttgt tgacctaact cctgtgttgg gatatcaata
    15061 ccgcctggcg caggcgttaa aataaacgac agcgtttcat tactataacc caccaccgct
    15121 aactgctgcg gaatagcaat atttttctct gctgccgcac gataaatact cattaatttc
    15181 aggctgtcag tagcaaacac tgcctcaggc aacggtgact ggcttaataa ttcccgcgcg
    15241 gcttgcagtg ctgtttcatg ggtataaccg ccgtcaacaa tccattcatc acgcactgca
    15301 atattatgcg cagccaggct ctgcttataa ccatttaccc gatccactga aacatggaca
    15361 tcaagcggag catgcaggca ggcaatattt tgatgcccac tttcaattaa tgcatcggtc
    15421 aacgcaatgc tgtcgccaaa attatcggta tcgacagaat aaacatgggc atattgacct
    15481 tcaactttgc caatcaccac gacaggaata tcgtatttat caagttgggc aaaaaatgac
    15541 tcatccgctg gcgaacttag cataataatg cctttaatca ttttctgctt aattttgctt
    15601 tcacattttt gtaagtcttc tgccggattg tgcgacgtct gcaatatcac gtcgaaacct
    15661 tcctcttcag ctttggcggt gatggcatgc aaaacttcag agaaaaacgg attacccgcc
    15721 gtagttttgg tcgaacgggt agaaatcacc ataatggcat caaaacccga agaggtcagc
    15781 gcgcgggcca gcttgtttgg ctgatactgt aattcttcaa tggcccgtaa cactttttcg
    15841 cgcgcttccg gggagatatt agtttgctta ttcagcacgc gtgatacggt ggattttgat
    15901 acgcctgcaa ccctggcaat atcataaata gtaggggaca taggtcagga actccgtccg
    15961 aacgtgaatg ctgcacatct tatggagttc gcccgtcgat gacaacacgg gcagttgata
    16021 atcaatggcc tggcccccac attcatatcc ttacgaatga tttttttctg gtcttcagag
    16081 cagcacagga cagcaatgaa gcgacttaaa aatgaactta atgcgctggt gaatcggggt
    16141 gtcgacagac atctgcgcct cgccgtaacc ggactaagcc gcagcggcaa aacagcgttt
    16201 atcactgcga tggtcaatca gttgctcaat attcacgccg gagcacgctt gccgctgtta
    16261 agcgcggtgc gtgaagagcg cctgctgggc gtaaaacgca ttcctcagcg tgactttggc
    16321 attccgcgct tcacatatga tgaaggactg gcgcagttat atggcgatcc tcccgcctgg
    16381 ccgacgccaa cgcgcggcgt cagtgaaatc cgcctggcgc tacgttttaa atcgaacgat
    16441 tcgctgctac gccacttcaa ggacacctcc acgctgtatc tggagattgt ggattatccc
    16501 ggcgaatggt tgctcgacct gccgatgctg gcgcaggact atttaagctg gtcacgccag
    16561 atgacgggct tactcaatgg tcagcgcggc gaatggtcgg tcaaatggcg aatgatgtgc
    16621 gaagggctgg acccgctagc gcctgccgac gaaaaccggc tggcagacat tgctgccgcg
    16681 tggaccgatt atctccacca ctgtaaacag caggggctgc actttattca gcctgggcgc
    16741 tttgtcttgc cgggagatat ggcaggtgcg cccgcgctgc aattcttccc gtggccggat
    16801 gtcgatgcct ggggcgagtc caaactggcg caggccgata agcacaccaa tgccggaatg
    16861 ctgcgcgagc ggtttaatta ttactgcgag aaggtggtga aggggttcta taagaatcat
    16921 tttctgcgct ttgaccgcca gattgtgctg gttgattgcc tgcaacctct caacagtggg
    16981 ccacaggcat ttaatgatat gcgtctggca ctgacgcagc tgatgcaaag tttccactac
    17041 gggcagcgta ccttgttccg gcgtttgttt tcgccggtta tcgataagct attgtttgct
    17101 gccactaaag cggaccatgt gaccatcgat cagcacgcca atatggtttc attactgcaa
    17161 caactgattc aggatgcctg gcaaaatgcg gcgtttgaag ggattagtat ggattgcctg
    17221 gggctggcct cggtgcaggc gaccaccagc ggcattattg atgttaacgg tgagaaaatc
    17281 ccggcgctac gcggtaatcg actcagcgat ggcgcaccgc tcactgttta tcctggcgaa
    17341 gttcccgctc gtttgcccgg tcaggcgttc tgggataagc aaggcttcca gtttgaggcg
    17401 tttcgtccgc aggtgatgga tgtcgacaaa ccgctgccgc atattcgcct tgatgccgcg
    17461 cttgaatttt taataggaga taaattgcga tgaccgaacc gttaaaacca cgtattgatt
    17521 tcgacggtcc gctggaggtc gatcagaatc caaaattcag ggcgcagcag acctttgacg
    17581 aaaatcaggc gcaaaatttt gccccggcca cgctcgatga agcgcctgaa gaagaggggc
    17641 aagttgaagc ggtaatggat gcagcgttac gtccgaaacg cagcctgtgg cgcaaaatgg
    17701 tgatgggcgg gctggctctg tttggcgcaa gcgttgtcgg gcagggtgta cagtggacaa
    17761 tgaatgcctg gcaaactcag gactgggtgg cgctgggtgg atgtgccgct ggggcattga
    17821 ttatcggcgc tggcgtaggt tctgtggtaa cagagtggcg gcgcttatgg cgcttgcgac
    17881 agcgcgccca tgaacgcgac gaagcgcgcg atttgttgca cagccacggc acgggcaaag
    17941 gccgcgcatt ttgcgaaaaa ctggcgcagc aggcgggtat tgatcagtct catccagcgc
    18001 tgcaacgctg gtatgcctca atccatgaaa cgcaaaacga ccgtgaagtg gtcagtttgt
    18061 atgcgcattt ggtccagcca gttttagatg cccaggcgcg gcgcgaaatc agccgttcgg
    18121 cggcggaatc aacgttgatg attgcggtca gcccgctggc gttggtcgat atggcgttta
    18181 tcgcctggcg caatctgcgt ttaattaatc gcatcgccac gctgtatggc attgaactag
    18241 ggtattacag ccgtttgcgt ctgtttaagc tggtattgct gaatatcgct tttgccggag
    18301 ccagcgaact ggtgcgcgaa gtggggatgg actggatgtc gcaagatctc gctgctcgtt
    18361 tgtctacccg cgcagctcag ggaattggtg ctggacttct gacggcacga ctcgggatta
    18421 aagctatgga gctttgccgc ccgctgccgt ggattgacga tgacaaacct cgcctcgggg
    18481 atttccgtcg tcagcttatc ggtcaggtga aagaaacgct gcaaaaaggc aaaacgccca
    18541 gcgaaaaata atgcaatatc gggtgctgac cggatatctt tacgccgaag tgcccgtttt
    18601 tccgtctttg tgtcaatgat tgttgacaga aaccttcctg ctatccaaat agtgtcatat
    18661 catcatatta attgttcttt tttcaggtga aggttcccat gcgtctggaa gtcttttgtg
    18721 aagaccgact cggtctgacc cgcgaattac tcgatctact cgtgctaaga ggcattgatt
    18781 tacgcggtat tgagattgat cccattgggc gaatctacct caattttgct gaattggagt
    18841 ttgagagttt cagcagtctg atggccgaaa tacgccgtat tgcaggtgtt accgatgtgc
    18901 gtactgtccc gtggatgcct tccgaacgtg agcatctggc gttgagcgcg ttactggagg
    18961 cgttgcccga acctgtgctc tctgtcgata tgaaaagcaa agtggatatg gcgaacccgg
    19021 cgagctgtca gctttttgcg caaaaattgg atcgcctgcg caaccatacc gccgcacaat
    19081 tgattaacgg ctttaatttt ttacgttggc tggaaagcga accgcaagat tcccataacg
    19141 agcatgtcgt tattaatggg cagaatttcc tgatggagat tacgcctgtt tatcttcagg
    19201 atgaaaatga tcaacacgtc ctgaccggtg cggtggtgat gttgcgatca acgattcgta
    19261 tgggccgcca gttgcaaaat gtcgccgccc aggacgtcag cgccttcagt caaattgtcg
    19321 ccgtcagccc gaaaatgaag catgttgtcg aacaggcgca gaaactggcg atgctaagcg
    19381 cgccgctgct gattacgggt gacacaggta caggtaaaga tctctttgcc tacgcctgcc
    19441 atcaggcaag ccccagagcg ggcaaacctt acctggcgct gaactgtgcg tctataccgg
    19501 aagatgcggt cgagagtgaa ctgtttggtc atgctccgga agggaagaaa ggattctttg
    19561 agcaggcgaa cggtggttcg gtgctgttgg atgaaatagg ggaaatgtca ccacggatgc
    19621 aggcgaaatt actgcgtttc cttaatgatg gcactttccg tcgggttggc gaagaccatg
    19681 aggtgcatgt cgatgtgcgg gtgatttgcg ctacgcagaa gaatctggtc gaactggtgc
    19741 aaaaaggcat gttccgtgaa gatctctatt atcgtctgaa cgtgttgacg ctcaatctgc
    19801 cgccgctacg tgactgtccg caggacatca tgccgttaac tgagctgttc gtcgcccgct
    19861 ttgccgacga gcagggcgtg ccgcgtccga aactggccgc tgacctgaat actgtactta
    19921 cgcgatatgc gtggccggga aatgtgcggc agttaaagaa cgctatctat cgcgcactga
    19981 cacaactgga cggttatgag ctgcgtccac aggatatttt gttgccggat tatgacgccg
    20041 caacggtagc cgtgggcgaa gatgcgatgg aaggttcgct ggacgaaatc accagccgtt
    20101 ttgaacgctc ggtattaacc cagctttatc gcaattatcc cagcacgcgc aaactggcaa
    20161 aacgtctcgg cgtttcacat accgcgattg ccaataagtt gcgggaatat ggcttaagcc
    20221 agaagaagaa cgaagagtaa gcgcgaatat gcctgatggt gcaacaccat caggcatatt
    20281 aacttatgct ttcagtacag ccagagctgc ttcgtaatcc ggctcggtgg tgatttcatc
    20341 caccagctgg ctgaaaatca cattgtcatt ttcgtcaata accacaacgg cacgcgctgc
    20401 cagacctttc agtgggccat cagcaattgc cacaccgtaa gcttgcagaa attcagcgtt
    20461 acggaaagtg gagagggtga taacgttgtt cagaccttct gcgccgcaga aacgagactg
    20521 ggcgaacggc agatcggcag agatacacag cacaacggtg ttgtcgatct cagttgccag
    20581 ttggttaaac ttacgtactg atgcggcgca aacaccggta tcaatactcg ggaaaatgtt
    20641 cagcactttg cgtttacccg caaactgacc gagggtgacg tcagacagat cttttgccac
    20701 gagagtaaaa gtctgcgctt tgctacccgc ctgcgggatg gaattggcga ctgtaaccgg
    20761 gttgccctgg aaatgaacgg tttgtgacat gattatcttt cctgtttaca tatagttaac
    20821 gtcacaccta gtttatgcta actgtcaata acacagcaaa cgctatttgc gcttaatccg
    20881 cagaccaccg cgacaacaag gagtaaagat gagaaccgtt aaggtatttg aggaagcctg
    20941 gcccttacat accccgtttg tgattgcccg gggaagtcgc agtgaagcgc gcgtggtggt
    21001 ggttgaactg gaagaagagg gtattaaagg caccggcgaa tgcacgccgt atccgcgtta
    21061 tggggaaagt gatgcctcgg taatggcgca aattatgagc gtcgtaccgc aattagagaa
    21121 agggctgaca cgggaggagt tgcaaaaaat tctccctgcc ggcgcagcac gtaatgcgct
    21181 ggattgtgca ttgtgggatc tggcggcgcg aaaacagcag caatcgctgg ctgatttgat
    21241 cggcataacg cttcctggga cagttatcac tgcacagacg gttgtcatcg gtacgcctga
    21301 tcagatggcc aatagtgcat cagcactctg gcaggcaggt gcgaaattac tgaaagtgaa
    21361 gctggataac catcttatca gtgagcggat ggtggcaatt cgcaccgctg tgcccgatgc
    21421 cacgctgatc gttgatgcaa atgaatcctg gcgtgcagaa gggttggcgg cgcgttgcca
    21481 gctattggcg gatttaggcg ttgcgatgct tgaacaaccg cttcctgcgc aggacgatgc
    21541 ggcactggag aattttattc atccgttgcc gatttgtgct gatgaaagtt gtcatactcg
    21601 tagcaatctg aaggcgctga aagggcgcta tgagatggtt aacattaagc tcgataaaac
    21661 cgggggtctg acggaagcgc tggcgctggc gactgaagca cgtgcacaag gttttcgtct
    21721 gatgctgggc tgcatgttgt gtacctctcg tgccattagc gccgctttac cgctggtgcc
    21781 gcaggtcagt ttcgccgatc ttgacggacc gacctggctg gcggtagatg tggaaccggc
    21841 gcttcagttc acgacgggcg aattgcatct ttaggatgcc agcgcagcaa gtttgccatc
    21901 gcaaagagat atttttcgct ggcttcgtcg gaggagatgg gcggaaactc tgcggtgatg
    21961 caatgcaagt tgagatctgc acaccaactg ccgaaagagc caggcgtttc atagccgacg
    22021 ctggttacca gaggcaattc aaacgcctgg gacagccatt cgcctaattc gctgtgtctg
    22081 ggatcttcaa tacaagccag tggatcgtgg aatgagacca cccaggcagg ttgaatgcgg
    22141 tgtataagct ggcacagcgc ctgggtttca ggttcggagc caggtttgtc acctgtcagt
    22201 aatacgacat cacgttcttc agcggcgctg ttccagcgat aaaccgtttc accttccttc
    22261 cagttcgccg ccggaaagtt tctgtttaaa tccacaccat tagcattggc ccgtaaccct
    22321 aactggcagc cgtcaggatt cacacacagc accacatgat ggcggcgcaa agaaggtgtc
    22381 aaagtccgca gagcacagga gagcgtgacg atcgaagagt tttcatcacc gtgagtgcca
    22441 gccagaatca aaccactttc gcgactggct gcaggggcag gaaaccagat taacggcgcg
    22501 cccaataatg aacgtccgta atgttctgtt ccgggcggaa atacgccgcg ttcggcgcgt
    22561 gggcgggtta cggtcatgat cgtctctggt tcaggaagta ttacaggcag tgttgtgcaa
    22621 attttgcgga ttatcaaatc cttttcttca acgctatgtc atcgatcttg tatcactttt
    22681 ttgccggaag ttgtttgcat tttcattgat gtgcagatat tagaattgtc gtaatgttat
    22741 atacatggcg tattttattc ttactggttc tacacatgtt aatcgcatcg gattgatcag
    22801 ttcaccttaa taagtttaat aaatcgtact gcacatagta cagtacgatg gtagttatgt
    22861 aattgattta catgaagaat ggcttatcac catcagtacc aggttgattg atgcctatat
    22921 tctcaccaat gtgtttctca ggtttatcga tgatatctgt gattaatgct gcgacactct
    22981 tacgtgatac gatcgtacct ttgaatggtt cattgcgtga ggtaagctca taatcaataa
    23041 tatcttcatc agtcagccag gcagggcgaa gaattgtata ttccagtcca gaagcctcaa
    23101 ttgcatccgc agccctgcgg aatggtttta ggggctcacc aataactgca ttattccatt
    23161 cgccaaattt tcctggaact tcatcataaa taccgaggga aagaacaaag atcagacgtt
    23221 taacatcaca ggctttcatc gcagcgatca ctgaatttgc ctgaatatct aaatcttccc
    23281 ccgtaaggtt ggcatacaca acgtcctgtc cctgcatggc ctgcttcagt gctgcatgat
    23341 tcagtacatc tcccataata atcttgctat tagttggata gggtttgtgg atttttgctg
    23401 gctgacgggc aaataaagtt tgtttaatgg tttgtttatc tgccagttga ttaatcacat
    23461 ggcgggctat ctgaccgcca gcaccaagaa tcagtacatt tttcataata tttctcatga
    23521 aacttatccc agatgggtgt tgaaaaaagg aataactttt tccagcgcct cctgaactgc
    23581 ttccggtttg tcatacagtt cataatgtga gaaatcgggt aatgaaacta actgacgatc
    23641 ctgcgaaacc atcgcccggc catagacttc cagtccatca cgataagcac cgaaggcacc
    23701 gactttttca ccaactacta ccttcaccgg ctgagtcagt aacacttcag taaatgcgaa
    23761 ggcatcccag gccagcgttt tctgtgcatg tgagaacaac atttttgtcg cacctcccgg
    23821 ttgttgcccg cgtggtgttt tatagtagtc agttgcttca taaacatcgc gttcggtcag
    23881 tccatgggct tttgctgcat ccaggctggc gggaagtaat tcgttgatct gtaattctcc
    23941 accacgagcc tctttggttc gttgcgacgc cattgcattc aatgcgccaa tcggatcgta
    24001 attgctgaaa ccttcccgaa aaagacgccc gatattaaca cccgtaatac tcactactgc
    24061 tttaattcgt ttttctgtca gcgcggcatt aatggcatag ccaccacccc cacacactcc
    24121 cagcacgcca atacgttcag cattaacata tggcagagtt actgcataat cgataacacg
    24181 gctaatatcc tctacgcgct gtgtcggatc ttctatccag cgaggctccc caccagattc
    24241 tccctggaaa ctggcgtcat aagcgagaac gagatagcct ttttcagcca atgctttgcc
    24301 atagatattg ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc
    24361 cggatactgt tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca
    24421 ggccatatca ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga
    24481 gctaatactg aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt
    24541 gcgccttcaa caatgtgata gcgtttatcc tggcttgatg cccgatcgta caggtcgtca
    24601 ctcatccatt tgctccctgc ctggctgccc gccacaatct gcatcggctg agtcaggtac
    24661 acttccgcca tatggtaagc atcataggta ataatctggt taaggctgcg caaagtagcg
    24721 taacccggtg ctgttggata ctgcgcgcga ggggtgtggt aatactccca ggcctgacgc
    24781 agttcttcgt tcggcgcatc ggactccttc attggtgcca gtggcataat ggcgtattct
    24841 ccgctgctaa tatcgctggt tctggcgttt gaacccgctt caacgtatgg cagggcatca
    24901 atagatttca cattgttttc ccaaccatta cggaacatcg aaccaatatt gaccgcacta
    24961 acggtaccga tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca
    25021 ccggcacaaa ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg
    25081 taatcaatca cggcactgat gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc
    25141 tcgccgccac tttcaccttg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc
    25201 gccagttttt tggcataggt tccggccgtt tgttctttaa cgcccccacc tgggtgagat
    25261 accacaattg cctgatactg acgggtttca tcaaattttg gagggaaata gatcactgca
    25321 gacaaagaga tggttggatt attgctgtta gtgaagctga ctttattatt catcattccg
    25381 ttcctcatgg agttgtcggt tcgttttaac ggttggtgat atcactatag atattgatca
    25441 ttaagttgat tagacccaaa atcatgatta gacctatcat ttaaatgatt aatagattgc
    25501 atggagatac tgaatgaagc gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc
    25561 agaggagcgt agcttcactc gtgcagcagc ccgcctgagc atggcgcagt cagctttaag
    25621 ccagatagtg cgtcgtatag aagaacgatt gggattgcgg cttctgacgc gaaccacgcg
    25681 cagcgttgtt ccaactgaag cgggcgagca tcttttgtct gttcttggcc ctatgttgca
    25741 tgacatagat tcagccctgg catccctgag cgagctgcag aaccgcccat ccgggacaat
    25801 acgtattact actgtagaac atgcagcaaa aacgatattg ttaccagcaa tgcgcacatt
    25861 cctgaaatcg catcctgaaa ttgatattca gctcaccatt gattatggtt tgaccgatgt
    25921 cgtttctgaa cgttttgatg caggcgtccg tctgggtggg gagatggata aagatatgat
    25981 cgccattcga atcgggccag atataccaat ggctattgtt ggctcaccgg attatttttc
    26041 tcgccgaagt gttccaacgt cagtgtcaca attaatagat catcaggcaa ttaatttgta
    26101 tcttcccaca tcgggtacag caaatcgctg gagattaata cgcggtggac gtgaagttcg
    26161 tgttcgcatg gaaggtcagc ttttactgaa tacgatagac ctgatcattg atgctgcaat
    26221 tgatgggcat ggattggcgt atctacctta tgatcaggtt gagcgggcta ttaaagaaaa
    26281 aaaactgata cgtgttttgg ataaattcac accagattta cccggttatc acctgtacta
    26341 tccacaccgt cgacatgctg gctcggcatt ctcattattt atagataggc tgaagtataa
    26401 aggtgctgtt tagcactact tgctgataca ttaatttaat ttctctctta acgtattctc
    26461 agttcctttc aacgttttgg ttatttttta ttcttcgtac aatggcgaca gatgctgatt
    26521 atgataccga aaactggttt gaacgtgcga agcctgaacg tagtgttcgg tgttctatgt
    26581 gctttaccgc attttggcgt cgattattta cactaaatct gatttaatat attgatacat
    26641 aaaacatttt atgcttcctg tgtcactttt ttgatggaag ttgtttgcat ttctttaagg
    26701 cgaaacaaat aattacgcat caattttaat gtcggttaga gggaaactta tgaagcactc
    26761 tgtttcagtc acgtgttgtg cgctgttggt cagcagcatt tctctttcgt atgctgcaga
    26821 agttccgagc ggcacagtac tggcagagaa gcaggagctg gtgcgccaca ttaaagatga
    26881 gcctgcgtcg ctggatcccg ctaaagccgt gggcctgcca gagattcagg tcattcgcga
    26941 tctgtttgaa ggactggtga atcagaacga gaaaggggag attgtccccg gcgttgcgac
    27001 tcagtggaaa agtaatgaca accgtatctg gacttttacc ctgcgcgata acgcaaaatg
    27061 ggcggatggc acaccggtaa cggcgcaaga ttttgtctac agctggcaac gtctggtgga
    27121 cccgaaaact ctatcgccat ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca
    27181 ggcgattatt gatggtaaag ctacgcctga ccagcttggc gtcaccgcag ttgatgccca
    27241 tactttgaaa attcagcttg ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt
    27301 tgccttcttc ccggtgcaaa aagccaacgt agaaagcggt aaagagtgga cgaaacccgg
    27361 aaatctgatt ggcaatggcg cttatgttct taaagagcgc gtagtcaatg aaaaactggt
    27421 cgtggtaccg aatacccatt attgggataa cgccaaaacg gtactgcaaa aagtgacctt
    27481 cctgccaatt aatcaggaat ccgcagccac taagcgttac ctcgcggggg atattgatat
    27541 caccgaatcc ttcccgaaaa atatgtatca gaagctgttg aaggatattc cggggcaggt
    27601 ttatacgccg ccgcagctcg ggacctatta ttatgcgttt aacacgcaaa aagggccgac
    27661 ggcagatcag cgcgttcgtc tggcattaag tatgacgata gatcgccgcc tgatgaccga
    27721 aaaagtatta gggacgggcg aaaagccagc gtggcatttt acaccagatg ttaccgccgg
    27781 atttacgccg gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcacaggc
    27841 aaaaactttg ttgagcgcag ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt
    27901 gtataacact tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa
    27961 gaaccttggc gtagatgtta aattgcaaaa tcaggaatgg aaaacctata tcgatagccg
    28021 taacaccggc aattttgatg ttatccgtgc ctcatgggtg ggggattata atgaaccctc
    28081 cactttcctg acattattaa cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc
    28141 ggcatacgac aaagttctgg cccaggcatc gacggaaaat accgttaaag cgcgtaatgc
    28201 cgattacaac gcggcagaaa aaatcctcat ggagcaagca ccgattgcac caatttatca
    28261 atataccaat ggacgattaa tcaagccgtg gctgaaaggt tatcccatta ataacccgga
    28321 agatgtggcg tacagccgga ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg
    28381 ttacccttgt ggataacgcc agtgatgtct gatttagcga ccctgttccg gcggtgtaat
    28441 gttatccata tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgtacaat
    28501 atcgataatc ttcaaataaa cgtcttgctg tgcagcaagc cactcagccc ataccgtggt
    28561 tttggtaaag caataaacca taatattcaa tgacgagtcg gcaaactggt tgaaataaac
    28621 cagtaaggtt tgtcgctggt cgatggccgg gtgatttttc agcatctcac gtacggcttc
    28681 gacaataacg cccacttttg ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg
    28741 gcggttggtc attcgtcctg ggttttctac gctgatcgat gaaaacagcg agttcggtac
    28801 gtacaatgga cgattatcaa aggtcgtaat tttggtaatt cgccagccaa tttccgctac
    28861 tgtaccttcg atatttctgt ccggtgaacg gatccagtcg ccaatactga aaggacggtc
    28921 gaaatagagc atgatcccgg aaaagaaatt actcagaata tctttaccgg ccataccgac
    28981 agccagacca ccaataccac caaaggtcag caagccagaa aggctcatgc cgaaatgttc
    29041 gccataaagc agaacaagca ccacaataat ggtgattttg ataatgcgcg acataatccg
    29101 cgcactggtg atatcgcgac cttttttaat ctgctgtttt tcaaactgat taatcagcaa
    29161 aaatagctta atcgtcagaa taaccgcaat cagg
//
LOCUS       DAPPNJ010000040        28587 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000040 DAPPNJ010000000
VERSION     DAPPNJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.040"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            21..245
                     /locus_tag="RPM73_001405"
     CDS             21..245
                     /locus_tag="RPM73_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDX6519779.1"
                     /translation="MNQNTQFQSLTDRVIREDECRKLTGVSRTTRYELEKKGGFPSRR
                     NLGGRSVGWLLSEVMDWVQSRDRVNSGKAA"
     gene            256..1302
                     /locus_tag="RPM73_001406"
     CDS             256..1302
                     /locus_tag="RPM73_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDX6519780.1"
                     /translation="MTYKTKATGGGRQWIHTKLERIANNATFAAGGQCDQSDLVRSKV
                     CNESFFLCSLRNFSRANEQGANLSDSCSCAIFLRRLLRVGDGVLVDSVSLMVLSCKAM
                     RLRSSNYGADSGYSCSLTLRRWSSSRATVTSCPISSAALTPIFSTCARASSEETRRFS
                     PLPSAWRRAISPRMAVTMNPALLSPSSFTDSMPSITSCGIRAVSDCDFAFFEPVAICN
                     PLNESVRQYTHKKSYIKALTCKPPINKVTYTLLMQGAETTKPRTVGAVTGLLTTNDSK
                     IIEAAMRNHTIHPQGRDSYNLNKFIWRFIALSTAQPRVIHIVATSEQEARQQSPAGCV
                     MVFAARIRQGVCHA"
     gene            1295..1615
                     /locus_tag="RPM73_001407"
     CDS             1295..1615
                     /locus_tag="RPM73_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519781.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKIYSAADGATDNISMGIASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            1622..1921
                     /locus_tag="RPM73_001408"
     CDS             1622..1921
                     /locus_tag="RPM73_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDX6519782.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFISRFSREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            1918..3735
                     /locus_tag="RPM73_001409"
     CDS             1918..3735
                     /locus_tag="RPM73_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDX6519783.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGFRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            4023..4268
                     /locus_tag="RPM73_001410"
     CDS             4023..4268
                     /locus_tag="RPM73_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519784.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            4265..4690
                     /locus_tag="RPM73_001411"
     CDS             4265..4690
                     /locus_tag="RPM73_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDX6519785.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     AMMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQTGQHDDPYGDNIPF"
     gene            4707..4955
                     /locus_tag="RPM73_001412"
     CDS             4707..4955
                     /locus_tag="RPM73_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519786.1"
                     /translation="MPVTFEEVQQHKKFHDFDDLETMTAKKYRRLLSSDALFVVDHHD
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKR"
     gene            complement(4991..5497)
                     /locus_tag="RPM73_001413"
     CDS             complement(4991..5497)
                     /locus_tag="RPM73_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519787.1"
                     /translation="MNLSAMVYPDTFIINGESFRGKRNAKENKVLIPYTNEPEVTIGQ
                     HIIQRVGKNEINLKIIDMKLLPNGTRRQGTNHPNMLTLYIENITGNEHMTPTKSNTFN
                     IGSISGDQVQIGEHNHMLVNISITELVEKVAKSGDVQAKSVLKQLLENSTVASIVGAG
                     ASALIGLL"
     gene            5784..6953
                     /locus_tag="RPM73_001414"
     CDS             5784..6953
                     /locus_tag="RPM73_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDX6519788.1"
                     /translation="MKKLIELRQQKNALKNQMRSLLEKADSENRSLNAEEGKQFDELR
                     AKADALDTEISRLESVADEERSKPGTGIQKLSSDELRNYIVTGDVRSLSTSTDSGRDG
                     GYTVIPELDREVMRQLQDDSVMRVIATVKTAKSNEFQKLVSTGGATVGRGTEGSARSE
                     TNTPKIERVTIKLNPIYAYPKTTQEILDFSEVDILGWLSSEIADTFASTEEDDFVNGD
                     GNGKPKGFMAYTRAATSDKTRAFGTIEKIVAASGTAITADELIDILYKLKAKYRKNAV
                     WVMNSGTAGTLQKLKNENGDYIWRDSLKEGAPDMLLGRPVYCLESMPDIGAGKAPLAV
                     GDFSRGYFIVDHVTGIRTRPDNITEPGFYKVHTDKYLGGGVVDSNAIKILEMKAG"
     gene            7008..7568
                     /locus_tag="RPM73_001415"
     CDS             7008..7568
                     /locus_tag="RPM73_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDX6519789.1"
                     /translation="MKNTDFEIRTSELTASNKKLVGYAVRWNSLSEIIWDEFREQFTP
                     GAFADYLATGNDVRCLYEHDYTQLLGRTKSGTLVLTEDNTGLRFELTPPDTQLGKDVL
                     TLVERGDITGMSFGFRALCEEWSIAQKPYLRTVTAAELREITITSMPAYPESGVEIAH
                     RSLFAQHPELRPTGNNRHRWSELAGL"
     gene            7570..8784
                     /locus_tag="RPM73_001416"
     CDS             7570..8784
                     /locus_tag="RPM73_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDX6519790.1"
                     /translation="MWWPFSRKKSEQRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGQDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPCTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYRQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGRKDE"
     gene            8777..9079
                     /locus_tag="RPM73_001417"
     CDS             8777..9079
                     /locus_tag="RPM73_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDX6519791.1"
                     /translation="MSEARITPDEVRAHLRLDDDLSGEGELLKMYTDAALEACQKHIG
                     KRFEDGLEFTPAMRVGCLMYIAFLYENREAVSPVEHSELPMAISALWSVYRDVGVY"
     gene            9079..9519
                     /locus_tag="RPM73_001418"
     CDS             9079..9519
                     /locus_tag="RPM73_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6519792.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDILFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            9503..9688
                     /locus_tag="RPM73_001419"
     CDS             9503..9688
                     /locus_tag="RPM73_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519793.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            9808..10164
                     /locus_tag="RPM73_001420"
     CDS             9808..10164
                     /locus_tag="RPM73_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDX6519794.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSLYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDRLA"
     gene            10148..11809
                     /locus_tag="RPM73_001421"
     CDS             10148..11809
                     /locus_tag="RPM73_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDX6519795.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFYDLENPLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIKLEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGA
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWVKAGWIRTTPGDCIDYDRIRDDIMVDAENFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRKVIAHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEEFAFDMSDSQKERLAAFDGV"
     gene            11815..12096
                     /locus_tag="RPM73_001422"
     CDS             11815..12096
                     /locus_tag="RPM73_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519796.1"
                     /translation="MTETDLLKIIRRITGISQQPDEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            complement(12591..12860)
                     /locus_tag="RPM73_001423"
     CDS             complement(12591..12860)
                     /locus_tag="RPM73_001423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519797.1"
                     /translation="MTWWSVTSLRWHYPDQVIRVFLTNFCYQLIINKFLSIQELKTSK
                     MTTQLTTHFVEALRTTTNNNKQKGNLLAVLLNRYHFSAIRYEAGG"
     gene            12891..13679
                     /gene="thiM"
                     /locus_tag="RPM73_001424"
     CDS             12891..13679
                     /gene="thiM"
                     /locus_tag="RPM73_001424"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDX6519798.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            13676..14476
                     /gene="thiD"
                     /locus_tag="RPM73_001425"
     CDS             13676..14476
                     /gene="thiD"
                     /locus_tag="RPM73_001425"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDX6519799.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHYHAWW"
     gene            14541..15359
                     /locus_tag="RPM73_001426"
     CDS             14541..15359
                     /locus_tag="RPM73_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDX6519800.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            15411..16157
                     /locus_tag="RPM73_001427"
     CDS             15411..16157
                     /locus_tag="RPM73_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX6519801.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(16131..17096)
                     /locus_tag="RPM73_001428"
     CDS             complement(16131..17096)
                     /locus_tag="RPM73_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX6519802.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(17093..18097)
                     /locus_tag="RPM73_001429"
     CDS             complement(17093..18097)
                     /locus_tag="RPM73_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDX6519803.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(18094..19371)
                     /locus_tag="RPM73_001430"
     CDS             complement(18094..19371)
                     /locus_tag="RPM73_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519804.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            19628..20680
                     /gene="fbaB"
                     /locus_tag="RPM73_001431"
     CDS             19628..20680
                     /gene="fbaB"
                     /locus_tag="RPM73_001431"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDX6519805.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            20988..21842
                     /gene="gatY"
                     /locus_tag="RPM73_001432"
     CDS             20988..21842
                     /gene="gatY"
                     /locus_tag="RPM73_001432"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDX6519806.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            21871..23133
                     /gene="gatZ"
                     /locus_tag="RPM73_001433"
     CDS             21871..23133
                     /gene="gatZ"
                     /locus_tag="RPM73_001433"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDX6519807.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            23143..23595
                     /gene="gatA"
                     /locus_tag="RPM73_001434"
     CDS             23143..23595
                     /gene="gatA"
                     /locus_tag="RPM73_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDX6519808.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETKLKEYFTKYVLDSDE"
     gene            23626..23910
                     /gene="gatB"
                     /locus_tag="RPM73_001435"
     CDS             23626..23910
                     /gene="gatB"
                     /locus_tag="RPM73_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDX6519809.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            23914..25269
                     /locus_tag="RPM73_001436"
     CDS             23914..25269
                     /locus_tag="RPM73_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HDX6519810.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            25317..26357
                     /gene="gatD"
                     /locus_tag="RPM73_001437"
     CDS             25317..26357
                     /gene="gatD"
                     /locus_tag="RPM73_001437"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDX6519811.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            26463..27236
                     /locus_tag="RPM73_001438"
     CDS             26463..27236
                     /locus_tag="RPM73_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX6519812.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(27318..28217)
                     /gene="yegS"
                     /locus_tag="RPM73_001439"
     CDS             complement(27318..28217)
                     /gene="yegS"
                     /locus_tag="RPM73_001439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDX6519813.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         7406 a         6743 c         7581 g         6857 t
ORIGIN      
        1 ttacgttcga gggtgttttt atgaatcaaa atacacaatt tcaatcacta acagatcgcg
       61 taattcgtga ggatgaatgt cgtaagttaa caggcgtcag ccgtactaca cgctacgaac
      121 tggagaagaa agggggcttt ccgtctcgcc gtaatcttgg ggggcgttct gtaggctggt
      181 tgctttccga agttatggac tgggtgcaaa gccgtgaccg cgttaattca ggtaaggcag
      241 cgtaaagggg agcatatgac atataaaaca aaggcgaccg ggggcggtcg ccaatggata
      301 cacactaaac ttgaacgcat cgccaacaat gccacatttg cggctggtgg gcaatgtgat
      361 cagtcagatt tggttcgttc caaggtttgc aacgagagct ttttcctgtg ctctttaagg
      421 aatttctcaa gagcaaacga acaaggtgcg aatctttctg attcatgttc atgcgctata
      481 tttttgcgtc gtctcttacg agttggtgat ggtgttttgg ttgattctgt gtcgctcatg
      541 gtgctgtcct gtaaagcaat gcgcctgcgt tcctcaaact atggcgctga tagtggctat
      601 tcctgctctt tgaccttgcg tcgctggagt tcttcacgcg cgacggtgac gagttgcccg
      661 atctcctcgg cggctttgac tccgattttt tccacctgcg ctagggcatc gagcgaagaa
      721 accaggaggt tttctccgct tccttctgcc tggcggcggg cgatttcacc gcgcatggcg
      781 gttactatga atccggcgtt gctttcaccg tccagtttta cggattccat gccttcaata
      841 acatcatgcg ggatccgagc tgtcagtgat tgtgattttg cgttttttga acctgtagcc
      901 atctgtaatc ctctcaatga aagtgtaaga caatatacac ataaaaagtc ttacataaaa
      961 gcattgacat gtaagccacc tataaataaa gttacttaca ccttgttaat gcaaggtgca
     1021 gaaacgacga aaccccgcac tgtaggagca gtaacggggc ttctaaccac caacgatagc
     1081 aagattatcg aggcagctat gagaaatcat accatacacc cgcaagggcg ggactcgtac
     1141 aacctgaata aattcatctg gcgttttatc gccctgagca ccgcacaacc gcgcgtgatt
     1201 cacatcgtgg ccaccagcga acaggaagca cgccagcaat ccccggctgg ctgcgtgatg
     1261 gtattcgccg cccgtattcg tcagggggtg tgccatgcct gatatgtcaa attaccagta
     1321 cctgattaat ccgcatttta actgtgagca tgatattgct aaaaagattt attccgctgc
     1381 ggatggggct actgacaata tatcaatggg tattgcgtca attggtagcc tgatgtggca
     1441 tgcgtcagaa aatgaggact atgacgaaaa ggccatgcgc attgatatgg gtaatatcgg
     1501 tttgttactg gcaatgcttg gacattttga tatttcgtta cggtgcacca ttgaaaatgc
     1561 cacagatgca ttaaatgcta taaagaaagc gaatactgat tcaaatcggg gataaataat
     1621 catgagaacg tatttatctg gcttgactgc cagcggttat gcacaccccc aaattatccc
     1681 cggcgctatt tatctggata agaacggtaa cagagtaacg gtaaaagaac tgatgtttga
     1741 ccgtgtgtat tttatccgtg atggctattc atttcttagt tcgctgaacg tggagatctt
     1801 tattagcaga ttcagtcggg aaatcccgct ttccagaaat aaccatgtgt catgtatgga
     1861 tgttgataaa aaactacagg aactaaaaaa catgattgcc gcgtggagag agcagaaatg
     1921 aaaaaagcgc caaatttaaa acaccagccg cgtgacaaaa tgacggaagt catcattttt
     1981 gcgggtagtg atgcgtgggc acatgcgaaa cagtggcagg aacaggacgg gcgactggct
     2041 ggcgataacg tgccacctgt ctggcttgga gagcaacaac ttgccgaact ggacaacctg
     2101 caaatcgtac cggacggacg ctatcgcgtg cgtctctatc aggcggggtt attgcgtccg
     2161 gggcttgtta ataccatcgg gcagaaactg gcagtggcag gtgtcaggga tgctgattat
     2221 taccctgaag gaatgcacag ccagaaacgg gagaactggc gcgaatatct ggaacgtgaa
     2281 cgggcagagc aggcggaaaa gaaaaaggta gttgaactgc ctgtaaagaa aaaagagcgg
     2341 gtaaaagacg ataacgcttc atcactggcg cttaaccaga tgggagcaag tcaacgcggc
     2401 gaagttctcc tggcacatta tggcggtgaa ctggcgattc atgctgactc tgacactgtt
     2461 caccattaca acggcgttgt atgggagcca gtacaggata aagaattaca gcgagctatg
     2521 gcacagattt tcattgatgc ggagatcagc tattcgcaga acgccattaa atcggcggtc
     2581 gataccatga agttaagttt gcctgtaatg gggaatacag cccgtaacct gattggattc
     2641 agtaacgggg tatttgatac cagaacaggt aattttcggg agcataacaa aaacgactgg
     2701 ttgttaattg ccagtgaatt acctttcagc ccaccagcag agggggaaac gctggcaaca
     2761 catgcgccga atttctggaa gtggttacgc cgttcggtgg ctgagaatga ccgcaaggcg
     2821 gatcgcgtac tggctgcatt attcatggtg ctggcgaacc ggtacgactg gcagttattc
     2881 attgaggtaa caggtccagg gggaagtggt aaaagcgtga tggcggagat ttgcaccatg
     2941 ctggcgggta aggccaacac agtatcggca agcatgaagg cgctggaaga tgcaagggaa
     3001 cgcgcgttag tggttggctt ttcgctgatt atcatgccgg atatgacccg ctacgctggt
     3061 gatggggcag ggattaaggc tattacaggc ggtgacaagg tggcaattga cccgaaacac
     3121 aaagccccct actcaacgcg tattccggca gtagtgctgg cggttaacaa taacgccatg
     3181 tcattcagtg accgcagcgg ggggatctca cgtcgtcggg tgatattcaa tttttcggaa
     3241 gttgtaccgg agaacgaacg cgatccaatg ctggcggaaa aaatagaagg tgagctggcg
     3301 gtagtgattc gccatctgct tacacggttt gctgaccagg acgaagccag acgcctgtta
     3361 tatgagcagc agaaatctga agaagcactg gcgataaagc gagaggggga ttcgctggtg
     3421 gacttctgcg gctatctcat ggcgtcggta atgtgtgatg gcctgttagt gggtaatgct
     3481 gaaattgtgc cattcagccc acgcaggtat ctctatcatg cctatctggc ttatatgagg
     3541 gcacatgggt ttggtaaacc tgtaacactg acgcgcttcg gtaaagatat gccgggggca
     3601 atggcggaat atggcaggga gtatatgaaa cggaaaacga agcacggttt tcgttcaaac
     3661 gtgacactga cggaggaatc agaagactgg atgccatcat gtgtatcggt cactaatgac
     3721 gatagcaaaa attaaactta ttgaataact gttcaccact gttcaccctg tcataattat
     3781 cttttatatc agtatattat agggtgagca gttatttatg aactgttcac caaactattc
     3841 actgttcacc tttttggttg tttattgagc ttcaagggtg aacagtggtg aacagttggt
     3901 gaatagtttt tgtgaaactg ttcacccctt aacagtatga atttaaatgg aaaatatcaa
     3961 aaggtgaaca ggtgaagggt taaaacgcaa aaattttaat ttactgcttt gagataaagc
     4021 ctatgacagc gaagcacaca aaaaaatcac aatcgcacgc acttgatttg acggaacact
     4081 ggttaagggt gtcgataaaa atcatcgacc gcaacgccgg ggaaggatat gcgaaagcac
     4141 atcccgaact gattagcgca ttcatgacaa cggcagctgc aaactttgcc acgctgacag
     4201 aacgggagat tgccgaagcg gaacaggtga caaccatcaa cgttaaaacc ggagagcaga
     4261 cagcatgaca gcacagatag cggcttacgg gcggctggtg gctgacccgc agttaaagac
     4321 caccagcaag ggtacacaaa tgacgatggc tagtatggcg gtcccccttc cgtgcagcca
     4381 ggcagatgac ggaacggcga tgatgtggtt atccgtcctg gcgtttggca gacaggccga
     4441 cgcactggca aaacaccaca aaggcgaact ggtgagcgtg gcgggtaaca tgcaggtaag
     4501 ccagtggaca ggccagaacg gcgaaacgcg gcagggctgg caggttatcg cagacagcgt
     4561 gatcagtgcg cgaacagcgc gaccgggcgg caaaaaaggc cagcaggggc aggccactga
     4621 cgcactgaac agggcaaaac agcagacggg tcagcacgat gatccgtacg gggacaacat
     4681 accgttttag cgacaacaga ggggacattc cagtgacatt tgaagaagtc cagcaacata
     4741 aaaagtttca tgattttgat gatctggaaa ccatgacagc aaaaaaatat cgccgtctgc
     4801 tttcttccga tgcgttgttt gttgtggatc atcatgattt tctgcgtagc tcactgaccg
     4861 gggaaatttt cgcaaccaac cgtgagcagg tggaagcgat gatcgaatat ctgtggaaaa
     4921 taaggcgcag aatgcgagat cccgtgaaac ggtaaaataa taaaggcctg gtaaaatcca
     4981 ggccattttt ttacagtaag cctattaggg cacttgcgcc agccccaaca atgctggcaa
     5041 cggtactgtt ttccagtaac tgctttaata ctgatttggc ttgtacatcc cccgatttag
     5101 cgactttttc aaccagttca gtgatgctga tgtttaccag catatggtta tgctcaccta
     5161 tctgcacttg atcaccacta attgaaccaa tgttaaatgt attactttta gttggcgtca
     5221 tatgttcatt tcccgtgata ttttcaatat agagagtcaa catatttgga tgattggttc
     5281 cctgtctgcg ggttccattg ggaagcaact tcatatctat aatttttaga tttatctcgt
     5341 ttttaccgac acgctggata atgtgttgac caatagttac ttccggttca tttgtatatg
     5401 gaattaatac cttgttttct tttgcgtttc ttttacctct gaatgattcg ccattaatta
     5461 tgaaagtatc aggatatacc attgcgctta aattcatgat gcgtcctttg ttgtagtcag
     5521 agacagcaat aataggccat ggtttacttc ttttgtaaat tatttgttcg tgttctttcg
     5581 tggttgttcg gtctgactga caggtgttta catactgatt tttatgtata tgttggagcg
     5641 tggcactcag acgtgagccg ccacaatgcc gcctgacccc ctgcgcgatg ccgggttgat
     5701 ctgcgagatg ccgagagtgt cgggcggcgc tccccccgtg ttggtttcac gtcctgaatc
     5761 ttaaccaata cgagaaaacc ttcatgaaga aattaatcga actccgccag caaaaaaacg
     5821 ccctgaaaaa ccagatgcga tccctgctgg aaaaagccga cagtgaaaac cgtagtctga
     5881 acgctgaaga aggcaaacag tttgatgaac tgcgtgcaaa agctgatgcc ctcgacacag
     5941 aaatttcccg cctcgagtct gtggctgatg aagaacgcag caagccagga acgggcatcc
     6001 agaaattatc atctgatgaa ttgcgtaact acatcgtaac cggagatgtg cgatcactgt
     6061 ccaccagcac tgacagcggc agggatggcg gatataccgt aattcctgag cttgatcgcg
     6121 aagtcatgcg ccagctacag gatgacagtg ttatgcgcgt gatcgcgacc gtgaagaccg
     6181 caaaatcaaa tgagtttcag aaactggttt ccactggcgg cgcaactgta ggacgaggca
     6241 cagaaggcag cgcacgcagt gaaaccaaca ccccgaaaat tgaacgcgta accatcaagt
     6301 tgaatccgat ctacgcctac ccgaaaacca cgcaggaaat cctggatttt tcagaggtgg
     6361 atattctggg ctggttatcc tccgaaattg ccgacacgtt cgccagcacc gaagaggatg
     6421 attttgttaa tggcgacggt aacggcaagc cgaaaggctt catggcttac acccgtgcgg
     6481 cgaccagtga caaaacccgc gcttttggca ccattgaaaa aatagtagcg gcaagtggaa
     6541 ccgccattac agcggacgaa ctgatcgaca ttctctacaa gctgaaagcg aaataccgca
     6601 aaaatgccgt ctgggtgatg aactcgggca cggcagggac actacagaag ctgaaaaatg
     6661 agaacggcga ttatatctgg cgcgacagcc ttaaagaagg tgcgccggat atgttgcttg
     6721 gtcgtcctgt ttactgcctg gagtccatgc cggacatcgg cgcaggaaaa gcaccgctag
     6781 cggttggcga tttcagtcgt ggttatttca tcgttgatca tgtaacaggg attcgcaccc
     6841 gaccggacaa cattactgaa cccggattct acaaggtcca cacggataaa tatctgggcg
     6901 gtggtgtggt ggattcaaac gccatcaaaa ttctggaaat gaaagctggc tagtcatgag
     6961 taaggaggag gctgcggcct cctttttcag ctttatggag tacaccgatg aaaaacaccg
     7021 attttgaaat ccgcacatct gaactgaccg ccagcaataa aaagctggtg gggtatgccg
     7081 ttcgctggaa cagcctttca gaaattatct gggacgaatt ccgcgaacag ttcacgccgg
     7141 gggcttttgc tgactatctg gcgacgggta atgatgtgcg ctgcctgtat gagcatgact
     7201 atacccaact gctggggcgc accaaatccg gcactctggt actgactgag gataacaccg
     7261 ggttacgttt tgaactgaca ccgccggata cccagcttgg aaaagatgtg cttacgctgg
     7321 tggagcgtgg cgacattaca ggaatgagct ttggttttcg cgcattatgc gaggagtgga
     7381 gtatcgcgca aaaaccgtat ctgcgtaccg taaccgccgc tgaactccgt gaaatcacaa
     7441 taacgtcgat gcctgcttat ccagaatctg gcgtggagat tgcccaccgt tcgttgtttg
     7501 cacagcaccc tgaattacgt ccgacaggaa ataatcgtca tcgctggtct gagctggcgg
     7561 ggttgtgata tgtggtggcc ttttagtcgt aaaaaaagcg agcagcgtaa cctgtccatt
     7621 gatgattttc tggcgctgtc cggcgtaccg aataccggat ccggagaata tgtttctgcc
     7681 gggacggctg aatcattgcc tgcagtgatg aatgcggttt ctgtcatcgc tgaggcggtg
     7741 gccacgatgc cgtgttatct gtatctggta cgtaatgaca agggcaggga ggcgcgggaa
     7801 tggctggaca gtcacccggt agatattctg ctgaatgagc agcctaattc gtgccagaca
     7861 ccttaccagt ttaaacgcac aatgatgcgt cactgcctgc tgaacggtaa cgcctatgcg
     7921 gttattgagt gggggcagga cgggcagcca aaatcacttc atccttatgc gccggggtgt
     7981 gttgtaccgg aacgcacagg cgcacacaaa taccgctata ccatcaccga accctgtaca
     8041 ggaacggtgc gcacgtattt acaggaagaa gttttgcatc tccgctatgc ctcggatgat
     8101 ggctttctgg ggcgttcccc cgtcacgatt tgccgtgagg cgctggggct tggccttgct
     8161 caacagcgtc acggagccag cattatgaaa gatggcatga tggcggcagg gattatcacg
     8221 tcaggcgaat ggctggacgg cgtgaaaggt aaacaggcat tggatgctct ggaacgctac
     8281 aagggggcga aaaatgccgg aaaaacgcca atccttgaag ggggtatgga ttacaggcaa
     8341 ctgggaatga gtaaccagga tgcggaatgg ctggcctccc gtcgcttctc cattgaagac
     8401 atcgcccgca tgttcaacgt atcgcctatt tttctgcagg aatacagcaa cagcacctac
     8461 agcaatttca gcgaggcaag ccgcgcgttt ctgaccatga caatgcgccc gtggctagcg
     8521 aacttcgaac agcaaatcaa ggccgctttg ctggtggctt ctcccgtacc tggtacccgt
     8581 tatctggttg agtttgattc agccgattta ttacgcgcca cacccaccga acgttatgcc
     8641 acgtatgaga aagggattaa gaacgggatc atgaatccga acgaagcccg tgagcgtgag
     8701 ggtatgccgc cgcgtgaagg tggtgatgag ttcagccagg catggaaaca gactgtggaa
     8761 attaaaggga gaaaagatga gtgaagccag aattacacct gatgaagtca gggcacatct
     8821 tcgacttgat gatgatttat ccggtgaagg cgaacttctg aaaatgtata ccgatgcggc
     8881 gctggaagcc tgccagaagc atatcgggaa acgttttgaa gacgggctgg aatttacccc
     8941 ggcaatgcgt gttggttgcc tgatgtacat cgctttcctg tacgagaacc gggaagcggt
     9001 ttcaccagtg gagcactcgg aactgcctat ggctatttct gcgctctggt cagtttatcg
     9061 tgacgtaggg gtgtactgat gccgtggcaa ccattaaggc gatgcactga gcctggctgt
     9121 aataagcgcg tgaagtccgg caagtgtgaa gagcacaggc gggctgcatg gcgtgcagag
     9181 gatgccagac ggggacaccg ccgcgcgcgc gggtattccc gacagtggga caaataccgc
     9241 gccctgtacc tgagcaaaaa cccgttatgc gtgcgttgtc tggctaaggg gatttatacg
     9301 ccagctcttg tggtggatca catcattccc atcaatggcg gcggtgatat tctcttctgg
     9361 cctgagtgga atcaccaggc attgtgccag acgtgccaca accgtaagac gacacgggaa
     9421 gatccagcca cgaaagcaaa ccgtaaagcg ggcatgtatc gcgagcagga agaacgggcg
     9481 gcacaccgta acgactggat gtatggcgat gatgactgaa caggagcaaa ccaggctgat
     9541 acgtggactg ataaggcagc gtgacacatg gaagacacag gagacagagc acaaagccaa
     9601 caggacaggg cgcacaaaac gcaccacagc gaagcgatta accgaccgtg accgcgaggt
     9661 catggaatgt tttcgcaatc gctggtgagg ccgtcagagg gggtgggggt ggttttcagg
     9721 acgaaaccgt ccctgccgga caccgaccgc cccctcaaat ttttgtgcac gggaattttt
     9781 ttgaaaataa ttgggcgaaa aaagaacatg gcaagaccac caaaagcccc cgcttacctg
     9841 gatgaaatcg cggtcaggca gtggaaggaa aaatcgcggc agctttccgg acgggaagac
     9901 cttacccccg ccgactggag caatctggaa ctgtattgcg ttaactactc cctataccgc
     9961 aaagccgtcg aagaccttgc gacgcgcggg ttcagcattg ttaacagtca gggcagcgag
    10021 agcagaaacc ccgccctgag cgcaaaggct gacgcggaaa gaataatgat caaaatggct
    10081 tctttgctgg gttttgaccc ggtaagccgt cgcagaaatc caccggaaac agaggaagag
    10141 gacgagcttg accgcctggc atgagtacgc agaaggcgta aaaaacggca aaattacggc
    10201 ctgtaaacga ctgaaacagg ccgttaaacg gtatttttat gaccttgaaa acccccttta
    10261 cacgttcgat ccggaggtcg tggagcggtt tattgccttt tccagggtgt gtccgcacgt
    10321 aaaaggcgca atgcgcggta gcccaattaa actggagccg tggcagcagt tcgcctttgc
    10381 gtgcatcctg ggctttaagg ttaaggccac cgggcggcgc aaatacacca gcgcattcat
    10441 tgaagtaccg cgaaaaaatg ccaaatccac ggtcgccgct atcctggcta actggtttct
    10501 ggttatggaa aacgggcagc aggatattta caccgccgcc gtgagtcgtg atcaggcgcg
    10561 gatcgtgttt gatgatgcgc gtcagatgtg ccttttatcc cgaccgttac gaaagcgggt
    10621 aaatattcag gcacacaagg tgatacaccc gaaaaccaac agtctgttaa agccactggc
    10681 agcaaaagcg gcaaccattg aaggaacaaa cccgagtctt gccattgtgg atgaatatca
    10741 cctgcaccct gacaacgggg tttattccgc gcttgaactg ggaatggggg cgcgtccgga
    10801 ggggttatta tttgccatca ccacatcagg gagcaacgtt gtttcagcct gtaaacaaca
    10861 ctacgactat tgctgccaga tactggatgg tgaagaagtg aacgaatcca tgtttgtgct
    10921 gatttacgag ctggatgatg aaagcgaggt tgacgatccg gcgatgtgga taaaggcgaa
    10981 tcccaatatc gatgtttccg tcgatcgtga aaaactggcc tcaaccatcc agaaagcgcg
    11041 gggtattccg tcgcagtggg tggaaatgct caccaagcga ttcaatatct ggtgtcaggg
    11101 ggctacgccg tggatgggta acggtgcatg ggcggagtgc gccggagcgt tcgcggaggc
    11161 ggatttatac gggcaggagt gctatgcggg gctggactta tcatcaacca gcgatatttc
    11221 cagcgtgtgc tatgcctttc cggtcggtaa aaagattatg ctggtttcac gtcattatct
    11281 gccggaattt cagctacaga accccgccaa taaaaaccgc gccatctatc gccagtgggt
    11341 aaaggcgggc tggatacgca caacaccggg tgactgcatt gattatgacc gtatccgtga
    11401 tgacatcatg gtggatgcag agaatttcaa tatcaggctg gtgggcttcg atacatggaa
    11461 cgccacgcac ctgaggacgc agctacaggg ggcaggattt gaggtggagc cgttcccgca
    11521 aacatacctt cgtttcagtc cggcggcgaa atcgttcgaa gtttttgtta accggaaggt
    11581 gattgcacat cgtggtgatc cggtgctggc ctggtcaatg agtaatgttg tgatgcagag
    11641 tgacgcgaac gccaatatca agccgaacaa gaaaaaatca tccaacaaga tagacccgag
    11701 cgttgcggcg ctgatggcgt ttggcacatt ccaggctgag catgaggaat ttgcatttga
    11761 tatgagcgac agccagaaag agcgacttgc ggcgtttgat ggggtatgac gagaatgact
    11821 gaaactgatc tactaaaaat aattcgccgc attaccggaa tcagtcagca gcctgacgaa
    11881 caggctacac agccggacag cgtgatagcc gaaaattacg cgcgtgtggt ggctgaggta
    11941 atgcgccgtg acggtattga gcttaacggc gtggatatgc gcaacatacg aaccagagtt
    12001 cttgagttgc tggcataccg tcgccgttct caacaacgga gggagagcgc gaaaaatacc
    12061 taccagtgga ggaagccgga gcacttacgg cggtaactgg ttgatattac cgaaagtgca
    12121 aaaatgcact tagcaactgg tggcgagttg cagatctgca actcgactat gaaactacgg
    12181 aaactacccg tagtttgggt agtaagagta acacccagat tttggggctt actcgcgata
    12241 cccaaataaa gggtatcggt gtaagaaata tcgtttctca tatgtgagca ccgagggcgg
    12301 aattccgcct tcggttactt attgtgctca tgcacaggga ggggcgggtc aaatctctgt
    12361 accctgacgt ctttcggact gccagcccca tcgatttttt atacccgcga aaaatgaaaa
    12421 acgcttcacg ctggtgggcc taatgccgat atggtgagcc acataccgga aatatccacc
    12481 agcacggcaa cggcagaata gccacaacac agaaaaccca cgaatatggg gtttttgtta
    12541 tggcatgggc atgatgccga tatggggatt cccatacgag ttatcagtga tcatccacca
    12601 gcttcatagc ggatagccga aaaatgataa cggttaagca aaactgccag caaatttccc
    12661 ttttgtttgt tattgttcgt cgttgttcgt agggcttcaa cgaaatgtgt ggtcagttgt
    12721 gtggtcattt ttgaggtttt taattcttgt atgcttaaaa acttattaat tatcaactga
    12781 taacaaaagt tagtaagaaa tacccgtatc acctgatctg gataatgcca gcgtagggaa
    12841 gtcacggacc accaggtcat tgcttcttca cgttatggca ggagcaaact atgcaagtcg
    12901 acctgctgag ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa cattcccctc
    12961 ttgtgcactg catgaccaac gatgtggtgc aaacctttac cgccaatacc ttgctggcgc
    13021 tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg
    13081 ccagtgcctt gttgattaac gttggcacac tgacgcagcc acgcgcacag gcgatgcgtg
    13141 ctgccgttga gcaagcaaaa agctctcaaa caccctggac gcttgatcca gtagcggttg
    13201 gtgcgctcga ttatcgccgc catttttgtc atgaattttt atcttttaaa ccgacagcga
    13261 tacgtggtaa tgcttcggaa atcatggcat tagctggcgt tgctaatggc ggacggggag
    13321 tggataccac tgacgccgca gctaacgcga tacccgctgc acaaacactg gcacgggaaa
    13381 ctggcgcaat cgtcgtggtc acaggcgagg tggattatgt taccgatggg catcgtatcg
    13441 ttggcattca cggtggcgat ccgctaatga ccaaagtggt aggaactggc tgtgcattat
    13501 cggcggttgt tgctgcctgt tgtgccttac caggcgatat gctggaaaat gtcgcatctg
    13561 cctgtcactg gatgaaacaa gccggagagc gcgcagtcgc cagaagcgag gggccaggca
    13621 gttttgttcc acatttcctt gatgcgctct ggcaattgac gcaggaggtg caggcatgaa
    13681 acgaattaac gctctgacga ttgccggtac tgatccgagt ggtggtgcgg ggattcaggc
    13741 cgatcttaaa accttctcgg cacttggcgc ttatggttgc tcagttatta ctgcactggt
    13801 ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt gagcctgatt ttgtcgccgc
    13861 ccagctcgat tcggtgttca gcgatgtgcg aatcgacacc actaaaatcg gtatgttggc
    13921 ggaaaccgat attgttgaag cggtggcaga acggttgcaa cgttatcaga tccaaaacgt
    13981 ggtactcgac accgttatgc tggcaaaaag cggcgacccg ctgctttcac cttcggcggt
    14041 tgctacgctg cgcagtcgat tattgccaca ggtttcatta ataacgccaa acttgcccga
    14101 agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc tggaacaagg
    14161 gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc atctggatga
    14221 tgagcaaagc ccggactggc tgtttacccg cgagggtgaa caacggttta ccgcaccgcg
    14281 cattatgacc aaaaacaccc acggcactgg ttgtacgctc tctgcggcgt tggctgcact
    14341 acgcccgcgc catacaaact gggctgacac cgtacaggag gcaaaaagct ggctttcatc
    14401 agcattagcc caggccgaca cgctggaagt tggtcacggt attggtccgg ttcaccacta
    14461 ccacgcctgg tggtgagcac cgtactatag ctgctttggt tattgtcagc ctttggcact
    14521 gacattcagg gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa ctcacctctc
    14581 tattatgcgc tcttgggcta atttccatcg ttgcaattta tccgcgtcag acggtgaatt
    14641 ttttctattc gacagcagtt cagattacag actacatcca tttctacggt tatcgcccgg
    14701 ttaaatcttt cgctattcgc attcctgcca gttacaccat tcacggaata gatgtttctc
    14761 gctggcagga gcggatcgac tggcagcggg tggcaaaaat gcgcgacaac ggtatccgct
    14821 tacagtttgc ttttattaag gcgacggaag gcgaaaagct ggtggacccg tatttttcgc
    14881 gtaactggca actaagccgc gaaaatggcc tgctgcgcgg ggcgtatcat tatttctccc
    14941 cgtcggtagc agcgccggtt caggcacgat tatttctgca aacggtggat ttctcacaag
    15001 gcgatttccc tgccgtgctg gacgtagaag aacggggaaa attatcggca aaagaattac
    15061 gcaagcgggt aagtcagtgg ctaaaaatgg tagaaaaacg cacgggaaaa aagccgatta
    15121 tttactcagg agccgttttt tatcacacga atctggcggg ctattttaat gaatatccgt
    15181 ggtgggtggc tcactattat caacgtcgtc cggacaatga cggaatggcc tggcgcttct
    15241 ggcagcattc cgaccgtgga caggtagatg gcatcaatgg tccggtggat tttaatgtgt
    15301 ttaatggcac ggtggaggag ttgcagggat tcgttgatgg gattaaagaa acgccttaaa
    15361 taagaagcag tgatatactc ccaccagact ccctggcagg aaaaagacaa atggaacaag
    15421 cgcataccca gctgattgcc caactgaacg agcgtatttt agcggcggat aacacgccgc
    15481 tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg ctggagcatg
    15541 gcaatatttt gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg cgcattacgg
    15601 tgcgcaaggc gatgcaggcg ctggaagaag agggtgtggt gacgcgttcg cgcggttacg
    15661 gcacgcagat caacaacatc ttcgaatact cgttgaaaga agcgcgaggt ttttctcagc
    15721 aggtggtatt gcgcgggaaa aagcccgata cgctatgggt taacaagcgt gtcgtgaaat
    15781 gtcccgaaga agtcgcgcag cagctggcgg tcgaagcagg aagtgatgtc tttttgctta
    15841 agcgtattcg ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg gttccggcgc
    15901 atttaattca tgatgttgat gccatcggga tttcacttta tgactatttc cgcagccagc
    15961 acatttaccc acaacgtacg cgttcccgcg ttagcgcccg gatgccggat gccgagtttc
    16021 agtcacatat tcagttagat agcaaaatac cggtgctggt gatcaagcaa gtggcgcttg
    16081 accaacagca acggcctatt gagtacagca ttagccactg tcgcagcgat ctatacgttt
    16141 ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg atgcccgaca
    16201 acccacgacg ccactgcatt gcccagtaat acggcatccg ccagtggcag accagaggcc
    16261 agcccggcaa gtacgccacc tgcatgactg tcgcccgccc caatggtgtc tacaacttgc
    16321 gttggaaatg ccggaatgca gccagaagcg tcgttgctga aataccatgc accctcttta
    16381 tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc aagtgttgtt
    16441 atctctgcgg ataaagcaaa acgttcggcg gcaatctcag cctcttgacg attgagtgat
    16501 actaaaggtc gacaggccat gatccgcgcc agtaatgcgt ccgggatatc gccgatacgt
    16561 gggccaaaat cgataaacgg cgtcacgtct tgcagctttt ccagccattc cactaacaat
    16621 tcgccgcagg gcgaggccag ttgataaccg gaaaaataga gcaggctgcc aggcgcaacg
    16681 gttaatcgcg ccagccactg gcgattccac tgattttcca caccgctgaa tgacataaaa
    16741 gtgcgttcgc catccggctc aaccagcgcc agacaccagc cgttatcgcc ttcggcgtta
    16801 tcgatcaggc tgattaaccc ctctttcgcc atccggttgc gaataatctc cgcccacacg
    16861 ccctgaccga gcggcaaggc attacccgct tcgatgccga ggcgctttaa cgccacggca
    16921 atattcagtg cgcagccgcc aacgttaacg ctctgctgct tcagttcgat atcgcaccca
    16981 cgccagggga gggcataagc gtcggcgatc acatcaatga ccgccgcgcc gacgaccatc
    17041 accggctgac gcgaggttaa ttcaggcagc agcgtgtgta atcgagcgcc gctcataccg
    17101 cctcccgttg ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa tcaagctgat
    17161 ttaccgcatc cagttccgcc tttaatgcag gatcgatagc gttaacgcca tgcaacgcgc
    17221 cgcaaatcgc cgtcgccata gcgccgatgg tgtctgtgtc gccgccaagg ttagcgcaca
    17281 ggacggcgca gcgattcggg tcggtttgtg ccagttcaac cagcgcaatg gcgcacggaa
    17341 cggactcaat agtgctggta cctgcgccaa cgacctggta aagctgttcg ctggcggatt
    17401 cggtgccgtc ggcattgcgc acaattttca gcgcaatctc cagacgtgct gccagtgagg
    17461 cgctgaaggt agtgatgcgt ttttgctgtg catgtcgcgc aattgaaggc agggaatcaa
    17521 caatcgctga ccagctttct ccgtcaatgg cacgagaaat cgcccatgcg atgactaccg
    17581 cacctgcaac tgccagatcg gatttatggg tcgggctcga cgccagcgcc acatcatcaa
    17641 taaaggaatc aacatcacgc gccggaagca aacaacctaa tggcgagacg cgcatcgctg
    17701 cgccgttggt cacgccgttg ttttccagtt cagcaacggg tttaccgtcg cgaatggcgt
    17761 taagcgcaat cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg aaacgcagcg
    17821 cccagtcgag aatattacgc ccaatcagat ccggatcgat cttgccttca cgttccagta
    17881 acgcatccgc cagacacagc gccatcgagg tatcgtcggt gaattcggcg cggttaaaat
    17941 aacaggccgc gttattctcc tttggtccag gcagaaaacg gtcaatccag ccaaagtgtg
    18001 ctttaacgcg gctgcgtggc caaagctcgg agggcatccc catcgcatcc cctaacgcct
    18061 gcccataaag agcaccgaga atacgttctg ttttcattta acttcccctt gtgtcaacgc
    18121 aatatcgcga tcatcgacct tgatagccgt aatttcgttg tcggattcgc ggaaaaaaat
    18181 catgaacagc acggcgataa tggcaatcat caccgcgccg aaagtccaca tcccggacca
    18241 gttgaaagtc agcccgttta ccggttccgg ataagcgaac atcttttcca tcatcacacc
    18301 gccaagacga tagccgagca aactgccgaa gccctggcag cagagcgtga tcagcccctg
    18361 cgcggcggta cgcatatgca cgggggcttt tttatcgaca tagatgtaag cggtaacgta
    18421 gtaaaaatcg taacttacgc cgtgaagcaa aataccgagg aacagtaacg cgtaggtgaa
    18481 atattcatcc gcactaccgt aaataaagaa gccatagcgg atcgcagcgg tgaccagacc
    18541 aagcaataat acctttttga taccaaagcg tttagtgaaa aacggcaatg ccagcataaa
    18601 gaagatttca gagaactggc cgagcgtcat ccagccggtg gcgtttttca tgccaacttc
    18661 ggtcagataa ccgttggcaa agatgtaata gaacgccagt ggcatcgcaa acaggaatga
    18721 acagaagaaa aagacgagga agtttttatc gcgcagcagg atcagcgcat ccaggccgag
    18781 catgacttta atgtccattt tgccggtgct ttttggcggc gtgtcgggca ggaaaaacgc
    18841 aaacacaccg agcagagcag aacttccggc ggtaatcagc agcgggatgt tagtcggtga
    18901 gatatcggca taccccagca tttgcggcaa gaaaccacat gccagaccag aggcaatcca
    18961 gccgatagtg cccatcacac gaatgcgcgg gaaatcacgc tccacatccg gcacgttggc
    19021 aaaagcgata ctgttagtca gcgcaatggt cggcatatag gttagagagt aagccagcag
    19081 taacggaaag aacccggcaa aagtggtctg ttgcgcagcg aaatacatca gcagcgcgcc
    19141 tgcgaacatc aataccgcca gcactttttg cgccgagaaa aagcggtcag tgatggagcc
    19201 aaccagaatt ggcgatagga tcgccgcaat ggcggtacag gcatacgacc agccaatttc
    19261 tccggcacta aaaccgcttt tacttaacca caaccacaat ggcacaaacc acgcgcccca
    19321 gataaaccat tcaacaaaca tcatgaacga cagctttgct gttgttttca tcttttaatt
    19381 ccttcatgac aggtaatgta aggtacgcga tgacaatacc acttaataat accttttaaa
    19441 tacctttgcg gtgaaaactt gaccgcgcta acattttttc tgatgaatcg agccaacaga
    19501 aaacgctgaa aaaacatcca aaagatggaa aaactcgccc gtaacgacca ggattatgat
    19561 tgcccgcaaa aggcgggcca ggacaatcca tagccgatat ccaatcggaa tttacgggag
    19621 catagtaatg acagatattg cgcagttgct tggcaaagac gccgacaacc ttttacagca
    19681 ccgttgtatg acaattcctt ctgaccagct ttatctcccc ggacatgact acgtagaccg
    19741 cgtaatgatt gacaataatc gcccgccagc ggtgttacgt aatatgcaga cgttgtacaa
    19801 caccgggcgt ctggctggca caggatatct ttctattctg ccggttgacc agggcgttga
    19861 gcactctgcc ggagcttcat ttgctgctaa cccgctctac tttgacccga aaaacattgt
    19921 tgaactggcg atcgaagcgg gctgtaactg tgtggcgtca acttacggcg tgctggcgtc
    19981 ggtatcgcgg cgttatgcgc atcgcattcc attcctcgtc aaacttaatc acaacgagac
    20041 gctaagttac ccgaatacct acgatcaaac gctgtatgcc agcgtggagc aggcgttcaa
    20101 catgggcgcg gttgcggttg gtgcgactat ctattttggc tcggaagagt cacgtcgcca
    20161 gattgaagaa atttctgcgg cttttgaacg tgcgcacgag ctgggtatgg tgacagtgct
    20221 gtgggcctat ttgcgtaact ccgcctttaa gaaagatggc gttgattacc atgtttccgc
    20281 cgacctgacc ggtcaggcaa accatctggc ggcaaccatc ggtgcagata tcgtcaaaca
    20341 aaaaatggcg gaaaataacg gcggctataa agcaattaat tacggttaca ccgacgatcg
    20401 tgtttacagc aaattgacca gcgaaaaccc gattgatctg gtgcgttatc agttagctaa
    20461 ctgctatatg ggtcgggctg ggttgataaa ctccggcggt gctgcgggcg gtgaaactga
    20521 cctcagcgat gcagtgcgta ctgcggttat caacaaacgc gctggcggaa tggggctgat
    20581 tcttggacgt aaggcgttca agaaatcgat ggctgacggc gtgaaactga ttaatgccgt
    20641 gcaggacgtt tatctcgata gcaaaattac tattgcctga tacgcctcat acgaacagca
    20701 ccaccccgta ggacggataa ggcgtttaca ctgcatccgg caacctatgc ctgatgcgac
    20761 gcggaagcgt cttatcatgc ctacatagca ctgccacgta tgtttacacc gcatccggca
    20821 taaaaacacg cgcactttgc tacggcttcc ctatcgggag gccgtttttt tgcctttcac
    20881 tcctcgaaca attttcatat tgtcgttttt gtgatcgtta tctcgatatt taaaaacaaa
    20941 taatttcatt atattttgaa atcgaaaaca aacgacagga tatgaaaatg tacgtggtat
    21001 cgacaaagca gatgctgaac aacgcacagc gcggcggtta tgcggttccg gcattcaata
    21061 ttcacaatct cgaaacgatg caagtggtgg tagaaaccgc tgccaacctg catgcgccgg
    21121 tcatcatcgc cggaacgcct ggcacattta ctcatgctgg tacagaaaat ctgttggcgc
    21181 tggtcagcgc gatggcgaag caatatcacc atccactggc aattcatctc gaccatcaca
    21241 cgaaatttga cgatatcgct cagaaggttc gttctggcgt gcgctcagtc atgattgacg
    21301 cctcgcattt gccttttgcg caaaatattt cacgggtcaa agaggtggtg gatttttgcc
    21361 atcgctttga tgtcagcgtc gaagcggagc tggggcaact tggcggccag gaagatgatg
    21421 tgcaagtcaa tgaagccgat gcgttgtaca ccaaccccgc acaggcgcgt gaatttgccg
    21481 aggcaaccgg gattgattcc ctggcggtag ccatcggcac ggctcatggt atgtatgcca
    21541 gcgcaccggc gcttgatttt tctcgactgg agaacattcg ccagtgggtg aacttaccgc
    21601 tggtgctgca tggcgcgtca gggttatcga ctaaggatat tcagcaaacc atcaaactgg
    21661 ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc cttctcgcag gcgttaaaaa
    21721 attacctgac cgagcacccc gaagcgaccg atccccggga ttatttgcag tcggctaaat
    21781 ccgcaatgcg cgatgtggtg agcaaagtga ttgccgattg tggctgcgag ggcagggcat
    21841 aacgcacctg ccatttaata aggaaaaaac atgaaaacgt taattgcccg gcataaagct
    21901 ggtgaacata tcggcatatg ttcagtctgt tctgcccatc cgttggttat cgaagcggcg
    21961 ctggcatttg atcgcaacag cacgcgcaaa gtgctgattg aagcaacgtc aaaccaggtc
    22021 aatcaatttg gcggttatac cggaatgaca ccggcagact ttcgcgaatt tgtttttacg
    22081 attgccgata aagttgggtt tgcacgcgaa cgcattattc tcggcggcga tcatctgggg
    22141 ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt cgagctggta
    22201 aaggaatatg ttcgtgccgg tttcagtaaa attcatcttg atgcgtcaat gtcctgcgcg
    22261 ggggatccca taccgttagc accagaaacg gttgcggaac gagctgctgt gctttgcttt
    22321 gctgcggaaa gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcacc
    22381 gaagttccgg ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt
    22441 gaagatgccg ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca
    22501 gaggcgttaa cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc
    22561 aatattatcc attatcagcc gcaggaagcg cagccgctgg cgcaatggat agaaagcacc
    22621 cgaatggttt atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta
    22681 gtccgcgatc actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgtgag
    22741 gcgatatttg cgctggcgca aattgagcag gaacttatcg cccccgaaaa tcgcagcggt
    22801 tgcctggcgg taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat
    22861 cgtacgggtt ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt
    22921 tattactggc cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa
    22981 ggcgtggaca tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt
    23041 cagtccgggg aattatcagc aataccgcat cagctgatta tggataaaat ttatgatgtt
    23101 ttgcgcgcct atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt
    23161 cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg
    23221 ctcgccaaag gtgtggttca tgatacctgg ccacaggcat taattgccag agaagcagaa
    23281 ttccctaccg ggataatgct tgagcagcac gccattgcaa taccgcattg tgaggcgatt
    23341 catgctaagt cgtcagccat ttatctgtta aggccaacaa ataaagttca ttttcagcaa
    23401 gcggatgatg ataacgacgt ggaggtatcg ttggttattg cgttgattgt tgaaaacccg
    23461 cagcagcaat tgaaactttt acgctgttta tttggcaagt tacaacagcc cgatatcgtc
    23521 gagacactaa tcactcttcc tgaaactaag ttaaaggaat acttcacaaa atatgtttta
    23581 gattcagacg aataaatccc tctgtaacaa taaaaaggac tatttatgaa acgcaagatt
    23641 attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga aattaaagag
    23701 ttgtgtcaga gtcataatat tcctgttgaa ttaatccagt gtcgggttaa tgaaatagaa
    23761 acctatatgg atggtgtgca tttgatatgt accactgcca gggtggatcg cagttttggc
    23821 gatattccgt tagttcacgg catgcctttt atttctggtg tcggtatcga agcattacaa
    23881 aataaaattc tgactatctt acaggggtga cctatgtttt cagaagtcat gcgttatatt
    23941 ctcgacctcg gccctacggt gatgcttccg attgtcatca ttattttttc taaaatatta
    24001 ggcatgaagg caggcgattg ctttaaagcg ggtctgcata tcgggattgg ctttgttggc
    24061 attggccttg tgattggctt aatgctggat tccattggtc cggcggcgaa agcgatggcg
    24121 gaaaatttcg acctgaatct gcatgtggtc gatgtcggct ggccgggctc ttcaccaatg
    24181 acctgggcgt cgcaaattgc gctggtggcg attccgattg cgattctggt taacgtggcg
    24241 atgttactga cccgtatgac gcgggtggta aatgttgata tctggaatat ctggcatatg
    24301 actttcaccg gcgcgttgct gcatctggca accggttcat ggatgatagg gatggcaggt
    24361 gtggtaattc acgcggcgtt tgtttataag ctcggcgact ggtttgcccg cgatacccga
    24421 aatttctttg agctggaagg tattgctatt ccgcacggta cgtcggcgta tatggggccg
    24481 attgcggtgc tggtcgatgc tatcatcgag aaaatcccag gcgttaaccg aattaaattt
    24541 agcgccgacg atattcagcg caaatttggt ccatttggcg agcctgtcac cgtgggtttt
    24601 gtgatggggc tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt attgcagctg
    24661 gcggtaaaaa cggcggcagt gatgctgcta atgccacggg tgattaaacc catcatggat
    24721 ggtttaacgc ccatcgctaa gcaggcccgt agtcgtttac aggcgaagtt cggcggtcag
    24781 gagttcctga ttggcctgga tcccgcatta ctgctggggc atacggcggt ggtatcggca
    24841 agcctgattt ttatcccgct caccatttta attgctgttt gtgttccggg taatcaggtg
    24901 ctgccgtttg gcgatcttgc caccatcggc ttcttcgtgg cgatggcggt cgccgtgcat
    24961 cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca tcatgagcat cactctgtgg
    25021 atcgcgacgc aaactattgg tttgcacacc caactggcgg ctaatgctgg ggcgttaaaa
    25081 gccgggggta tggtggcttc aatggatcag ggcggttctc ccattacctg gttactgatt
    25141 caggttttct ccccgcaaaa tattcccggt ttcattatta tcggtgcaat ttatctgacc
    25201 ggtattttca tgacctggcg tagagcgcgt ggctttatta aacaagagaa agtcgttctc
    25261 gcagaataat ttttacctga gggggaggtt atccccctta acaatcagga gtttttatga
    25321 aatcagtggt gaatgatact gatggtatcg tgcgcgttgc agaaagcgtc attcctgaaa
    25381 ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc gggcttatgt ggttccgatt
    25441 tacccaggat atttaaaaat ggtgcacatt attatccaat aacgttaggc catgaattta
    25501 gcggctatat tgatgcggtg ggatccggtg ttgatgattt acaccctggc gatgcggttg
    25561 cctgtgtgcc gttattaccc tgttttactt gtccagagtg tctgaaaggg ttttattccc
    25621 agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg tggatttgct gaatatattg
    25681 tcgttaagcg aaaaaatgtc tttgctctac ccacggatat gcctattgag gatggggctt
    25741 ttattgagcc gattaccgtt ggtctgcatg cttttcattt agcgcaaggt tgtgagaata
    25801 aaaacgttat tattattggt gccggaacca ttggcctgct ggctattcag tgcgctgtcg
    25861 cgctgggagc aaagagtgtg acggctatcg acattagctc agaaaaactg gcactggcaa
    25921 aatctttcgg tgcgatgcaa acatttaaca gtagcgaaat gagcgcgccg caaatgcaga
    25981 gcgttttacg cgaactgcgt tttaatcagc ttatcctcga gacggctggc gtaccgcaaa
    26041 ctgtcgaact ggcggtagag attgccggtc ctcatgccca actggcgctg gtgggcacgt
    26101 tgcatcagga tctgcattta acatcggcaa cgtttggcaa aatattgcgt aaagagctga
    26161 cggttatcgg cagttggatg aactattcca gcccttggcc ggggcaggag tgggaaacgg
    26221 cgagccggtt gctgacagaa cgtaagttaa gcctggagcc attaatcgct caccgtggaa
    26281 gctttgaaag cttcacccag gcggtgcgtg acatcgctcg taatgccatg ccgggcaaag
    26341 tgttgctcat tccatgaaac cgcgggccag cgtgatgctg gcccggtatt gtgcaaaaca
    26401 gatcattcac caatggtccc ccttcgttta cactagccac aattgaatat gggtaaatga
    26461 cgatgaattc attcgagcga aggaataaga tcatccaatt agtgaatgaa cagggaaccg
    26521 tgcttgttca ggatctggcg ggagtatttg ctgcctcgga agcgacaatc cgtgccgatt
    26581 tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca tggcggtgcg gcgaaaataa
    26641 tgtccggtaa tagtgaaacc gagacccagg aagtcgggtt taaagagcga tttcagctcg
    26701 ccagcgcgcc caaaaacaga atagcgcagg cggcagtcaa aatgatccat gaagggatga
    26761 ccgttattct cgacagcgga agtaccacaa tgcttatcgc cgaaggatta atgaccgcta
    26821 aaaatatcac ggtgattacc aacagtctcc cggctgcgtt tgccctttcc gaaaataaag
    26881 acattactct ggtcgtctgc ggtggcacgg tccgccataa aacgcgatct atgcatggat
    26941 ctattgctga acgttccctg caggatatta atgccgattt aatgtttgtc ggtgctgatg
    27001 gtattgatgc ggttaatggt attacgacct ttaatgaagg ttattcgatt agcggtgcga
    27061 tggtcaccgc cgctaataaa gtgattgccg ttctcgattc atcgaaattt aaccgtcgcg
    27121 gttttaatca ggtactgcca atcgaaaaaa ttgacattat tattaccgat gatgctgtat
    27181 cagaggtgga taaactggca ttgcagaaga cacgggtaaa attaattacg gtgtagtatt
    27241 gttctgtatg ccggataagg tatttacacc gcatccggca tttcggattc tccctggttc
    27301 aagagagaat agtttgatta acgcaataat ggacaatctg gtggtaatcg acaacgcaac
    27361 gccgccggaa gtatttcaat atgaaagttt tgtccgctca acggttcgcc atcaagatta
    27421 aaagtgattt cgtgtggggc ttgtatatca aaccacgacg aagcgccttc gataatattc
    27481 gggttatctt cgtcagattt taaggttgat acgagagccg gaagaatttc atcgccggta
    27541 aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt tcggacacaa ttgctgaccg
    27601 ccaccggcct gacgcccgtt accaatacca atgaccaggg cgtcaccttg ccagtgaaag
    27661 ttttcaccgc ggatttcaca acggtccggt tgcagagtat ccatgcgcat taagccatga
    27721 atgatgtaag agacgccacc cagcgcggct tttaattttt ccggcgtttc tgtggtaata
    27781 cgcgtcccaa atccgcctgt cgccatatta ataaaacagg tttgtttgtt gacctgcgcc
    27841 atatctatcg caatggcgtt accggcaatt gccagtttca gcgccttatc cagtgcctca
    27901 ggaatcccta cactggtggc aaaatcattg gcggttccta atggcaaaat tcccagcgcg
    27961 ggtatgtcat ccccctcaca ctgaatcaac gccgtagaaa cttcattaat ggtgccatcg
    28021 ccaccaccgg caatcaccgt tgcgacgccc aacttccggg cctcctctac atatcgtgcg
    28081 gcatcgcctt tctcccaggt gacccgcaca tggatcgtca ttccttcctc acgcaacagc
    28141 ataattgctt cgcgcaaggg tagattgtca gtacttttgc cattaagaat cagtaagctg
    28201 gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg gtctgtagat agtgtagagc
    28261 agaaaacaaa aggtggagtc agaacaggat gaaagtcgga ggataaaaag tcagttcaag
    28321 tatggaggat tcagtggtta ccaccaggag cttgcaatgg ggacagcgaa tactaacggc
    28381 tgccaccgat aaatttcaaa aaagagcata tgcctaatat tcaaccaaac agtggcatct
    28441 taaatatact attccaaata tattaaagcc cccatggagt taccctgaag ggcctcaatg
    28501 tccgtaattc ctacttatgt aggaaatgtt gtacagaaca tttattataa tcctattcaa
    28561 ttataataat catgccatta ttatatt
//
LOCUS       DAPPNJ010000041        28236 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000041 DAPPNJ010000000
VERSION     DAPPNJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.041"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(86..1735)
                     /gene="actP"
                     /locus_tag="RPM73_001440"
     CDS             complement(86..1735)
                     /gene="actP"
                     /locus_tag="RPM73_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDX6519814.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1732..2046)
                     /locus_tag="RPM73_001441"
     CDS             complement(1732..2046)
                     /locus_tag="RPM73_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDX6519815.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2246..4204)
                     /gene="acs"
                     /locus_tag="RPM73_001442"
     CDS             complement(2246..4204)
                     /gene="acs"
                     /locus_tag="RPM73_001442"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDX6519816.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4596..6032
                     /gene="nrfA"
                     /locus_tag="RPM73_001443"
     CDS             4596..6032
                     /gene="nrfA"
                     /locus_tag="RPM73_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDX6519817.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6077..6643
                     /gene="nrfB"
                     /locus_tag="RPM73_001444"
     CDS             6077..6643
                     /gene="nrfB"
                     /locus_tag="RPM73_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDX6519818.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6640..7311
                     /gene="nrfC"
                     /locus_tag="RPM73_001445"
     CDS             6640..7311
                     /gene="nrfC"
                     /locus_tag="RPM73_001445"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDX6519819.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7308..8264
                     /gene="nrfD"
                     /locus_tag="RPM73_001446"
     CDS             7308..8264
                     /gene="nrfD"
                     /locus_tag="RPM73_001446"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDX6519820.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8284..10002
                     /locus_tag="RPM73_001447"
     CDS             8284..10002
                     /locus_tag="RPM73_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDX6519821.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9995..10378
                     /gene="nrfF"
                     /locus_tag="RPM73_001448"
     CDS             9995..10378
                     /gene="nrfF"
                     /locus_tag="RPM73_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDX6519822.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10375..10971
                     /gene="nrfG"
                     /locus_tag="RPM73_001449"
     CDS             10375..10971
                     /gene="nrfG"
                     /locus_tag="RPM73_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDX6519823.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11313..12626
                     /gene="gltP"
                     /locus_tag="RPM73_001450"
     CDS             11313..12626
                     /gene="gltP"
                     /locus_tag="RPM73_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDX6519824.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPIGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12704..13393)
                     /locus_tag="RPM73_001451"
     CDS             complement(12704..13393)
                     /locus_tag="RPM73_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX6519825.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13487..15634)
                     /gene="fdhF"
                     /locus_tag="RPM73_001452"
     CDS             complement(13487..15634)
                     /gene="fdhF"
                     /locus_tag="RPM73_001452"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15215..15217),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDX6519826.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15832..17298)
                     /gene="mdtP"
                     /locus_tag="RPM73_001453"
     CDS             complement(15832..17298)
                     /gene="mdtP"
                     /locus_tag="RPM73_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDX6519827.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGFDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17295..19346)
                     /gene="mdtO"
                     /locus_tag="RPM73_001454"
     CDS             complement(17295..19346)
                     /gene="mdtO"
                     /locus_tag="RPM73_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDX6519828.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLVIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19346..20377)
                     /gene="mdtN"
                     /locus_tag="RPM73_001455"
     CDS             complement(19346..20377)
                     /gene="mdtN"
                     /locus_tag="RPM73_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDX6519829.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYKANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20396..20671)
                     /locus_tag="RPM73_001456"
     CDS             complement(20396..20671)
                     /locus_tag="RPM73_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519830.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVAIIYTALFALYAMLFWLAFF"
     gene            complement(20880..22865)
                     /gene="yjcS"
                     /locus_tag="RPM73_001457"
     CDS             complement(20880..22865)
                     /gene="yjcS"
                     /locus_tag="RPM73_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDX6519831.1"
                     /translation="MNNSRLFRLSRIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(23138..24067)
                     /gene="alsK"
                     /locus_tag="RPM73_001458"
     CDS             complement(23138..24067)
                     /gene="alsK"
                     /locus_tag="RPM73_001458"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDX6519832.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(24051..24746)
                     /gene="alsE"
                     /locus_tag="RPM73_001459"
     CDS             complement(24051..24746)
                     /gene="alsE"
                     /locus_tag="RPM73_001459"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDX6519833.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(24757..25737)
                     /gene="alsC"
                     /locus_tag="RPM73_001460"
     CDS             complement(24757..25737)
                     /gene="alsC"
                     /locus_tag="RPM73_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDX6519834.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(25716..27248)
                     /gene="alsA"
                     /locus_tag="RPM73_001461"
     CDS             complement(25716..27248)
                     /gene="alsA"
                     /locus_tag="RPM73_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDX6519835.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLVHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITKLSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(27375..>28236)
                     /gene="alsB"
                     /locus_tag="RPM73_001462"
     CDS             complement(27375..>28236)
                     /gene="alsB"
                     /locus_tag="RPM73_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDX6519836.1"
                     /translation="EYAVVLKTLSNPFWVDMKKGIEDEAKTLGVSVDIFASPSEGDFQ
                     SQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVARAWKKGIYLVNLDEKIDMDNLKKAGG
                     NVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVAIIEGKAGNASGEARRNGATEAFKKA
                     SQIKLVASQPADWDRIKALDVATNVLQRNPNIKAIYCANDTMAMGVAQAVANAGKTGK
                     ILVVGTDGIPEARKMVEAGQMTATVAQNPADIGATGLKLMVNAEKSGKVIPLDKAPEF
                     KLVDSILVTQ"
BASE COUNT         6473 a         7397 c         7522 g         6844 t
ORIGIN      
        1 ttatttgttt aatattttgg gaaaaggtga tgcatgctgg agaggaagag tattccccgg
       61 tcaggcgacc ggggaagggg agagattaat gtgcgcggcc ttgctcaacg ccaaagccgg
      121 tctgggagcg gataaactgc gcgcggaaca gttcacgctc gcgcgcccct tccgctgagt
      181 tatcggttgc cgagaagaac cagatgccga ggaatgccac ggtgatagag aacagcgccg
      241 ggtattcata cgggaagatg gctttttcgt gaccaaggat ctgtacccaa atcgtcgggc
      301 cgaggatcat cagtactact gcggtaatca gccccagcca gccacccatc atcgcgccac
      361 gcgtggtcag tttcgaccag tacatagaaa gcagaatgat cgggaagtta cagctcgccg
      421 cgatggcaaa cgccagcccc accataaagg cgatgttctg attctcaaac agcacgccga
      481 ggataatggc aatcacaccc aaaatcagca cggtgatttt cgatacccgc agttcttcac
      541 gttcggtcgc gccttttttg aagacgttag cgtacaggtc atgcgaaacc gctgatgcgc
      601 ccgccaacgt cagacccgca accaccgcaa gaatggtggc aaaagcaact gctgaaataa
      661 aaccgaggaa caggttgccg cccactgcat tcgccaggtg aacggccgcc atgttgttac
      721 caccaatcag atggcccgcc gcgtctttat attccggatt cgcaccaaca agcatgatcg
      781 cgccgaagcc gataataaag gtcagaatat agaagtagcc cataaatccg gtggcgtaga
      841 acacgctctt acgtgcttcg cgggcatcgc tgactgtaaa gaagcgcatc agaatgtgcg
      901 gcaagcccgc cgtaccaaac atcagtccca gccccagaga gagcgcggag atcggatctt
      961 tcaccagtcc gcccggtttc atgatgtcga cacctttcgg gtgtaccgcc atcgcttcgc
     1021 tgaacagatt gttgaagcta aagccgacgt gtttcatcac cataaaggcc ataaagctgg
     1081 caccgaacag caacagaacg gctttaataa tctgtaccca agtggtcgcc agcatgccgc
     1141 cgaacaggac gtacatcatc atcagcacgc cgaccagcac caccgcaatg tgatagttaa
     1201 ggccaaacag cagctcgatc agtttacctg cgcccaccat ctgggcgata aggtaaagcg
     1261 ccaccaccac cagagaacca caggccgaaa gaatacgaat cggcccttgt ttcagacggt
     1321 aagaggccac atcggcaaag gtgtagcgcc ccaggttacg cagacgttcg gcaatcagga
     1381 acaaaatgat cggccagccc accaggaagc ccagcgagta aattaagccg tcatagccgg
     1441 aggtaaacac cagcgcggag atccccaaga atgaggcggc ggacatatag tccccggcaa
     1501 tcgccagccc gttctggaag ccagtgatat tgccgcctgc ggtgtagtag tcgctacgag
     1561 aacgtacgcg ttttgatgcc cagtaggtaa tgccgagcgt aaacacgacg aaaatcagga
     1621 acataataat cgcctgccag ttcgttggct ggcgctctac ggccccgcta atagcatccg
     1681 cggcgttagc tgcgaaaggg agtgtggcgg caagcgccgt cagaactctt ttcatgatgc
     1741 ttgtacctca tgcaggactt cattattaag acggtcgaat tcgccgttcg cccgccagat
     1801 gtagataccg gtgagaacaa aggagatcac aatcactcca acaccaattg gaataccgcg
     1861 tgtgacgctg gtgttcggat tcagcggcgt gcccagccag ccgggcgcga aggcgatcag
     1921 taaaataaag ccgatataaa ctgccagcat aataatcgac aggatggtgg caaaccgttg
     1981 ccgtttttcg actaactccc tgaaatgcgc attgtcttct atccgctgat aaatagtgcc
     2041 attcatcaca gattctccag aggtaatgta gggattgttt taattcccgc tcccttatgg
     2101 gagaaggtta acgctcgggt aacccttgcc gaatgtaggc cggataaggc gtttacgccg
     2161 catccggcaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta
     2221 ccgactcgca tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa
     2281 gcagcttctc gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat
     2341 cgcccgccgc aattttgcgc agaatacggc gcataatttt gccggagcgg gttttaggca
     2401 gggagtcggt ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc
     2461 agttgcggac ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt
     2521 aggcgtagat cgcctgacct ttaatattgt gcggaatacc tactacggcg gcttcggcaa
     2581 tcttcggatg cgccaccagc gccgactcaa tctctgccgt ccccagacgg tgaccggaga
     2641 cgttcagcac gtcgtccaca cgcccggtga tccagtaata gccatcttca tcgcgacgcg
     2701 cgccgtcgcc gctgaaatac atatttttga aggtggagaa gtaggtctgt tcaaaacgtt
     2761 cgtgatcgcc aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctgc
     2821 cttcggtagc gccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga
     2881 agaacggacg tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggcgtgatca
     2941 tgaaaccgcc agtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga
     3001 tttttttcca gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa
     3061 tgcgcagcga cgaacggtcg gtaccctcga tcgctttatc gccttccgcc atcagcgcgc
     3121 ggatcgccgt gggtgcggta tagagaatat tgacctgatg cttgtccacc acctgcgcca
     3181 tacgggcagg cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg
     3241 ccagcgggcc gtacagcaag taactgtgtc cggtcaccca gcccacatcg gcggtgcacc
     3301 agtagatatc acccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca
     3361 gataaccgcc ggtagtatgc agcacacctt ttggcttacc ggtagaaccg gaggtgtaga
     3421 gaataaacag cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct
     3481 caaccaggtc gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac
     3541 gcttcagtac caccacatgc tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa
     3601 cgtttttctt cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca
     3661 gtcgtgagtt ggaatcaata atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa
     3721 tcaccgaatg caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg gcttccggca
     3781 ccatcggcat ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat
     3841 tggcgaagcg gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg
     3901 cgtcgtcgcc ttcccagatg atggcggtgc gatcgccgtt ttcttgcaga tggcggtcaa
     3961 ggcagtttgc cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg
     4021 gggcaaagga ggtatttttc accttctggt aaggtttgat ccagtcgaga atttttccct
     4081 gttcgcccca gaaggtatca ggtacgttaa tagattgttg atacatcgcc tcgtactgct
     4141 gagggtttat caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc
     4201 tcatgctttt gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg
     4261 ggtattggca gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa
     4321 ataaacggca gggtaatttt tgaaggtcag caacaaaagt tgattaattc tttgaggaac
     4381 atgcagttat gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt
     4441 tacatgcact tacaattgat taaagacaac attcacagtg tggttatttg ttacacatag
     4501 gggcgagcaa tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaacaa taaaaaccgc
     4561 cattgcaaca atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac
     4621 gccgtatctt cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa
     4681 cggcagctcc cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc
     4741 atcccgatca atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc
     4801 tggcggaaga tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca
     4861 acaagccgcg tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg
     4921 cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg
     4981 atgtggcgcg tctgatccag aaagacggcg aagatggcta cttccacggt aagtgggcgc
     5041 gcggcggccc ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac
     5101 cagagttcgc caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga
     5161 tggaagccat tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt
     5221 gcggtcagtg ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt
     5281 gggatgacgg catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg
     5341 actggactaa ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa
     5401 cctggacagc gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa
     5461 aagtgcagaa cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata
     5521 acttcgccca gacttgtgcg aactgccata cccaggacaa agctgccttg caaaaagtgg
     5581 tcgcggaacg taagcagtcg attaacgacc tgaaaatcaa ggttgaagat caactggttc
     5641 acgctcactt cgaagcgaaa gcagcgctgg atgcaggcgc gacggaagcc gaaatgaagc
     5701 caattcagga cgatatccgt catgcccagt ggcgctggga tctggcgatc gcttcccacg
     5761 gcattcatat gcacgcaccg gaagaaggtt tacggatgct cggtacggcg atggataaag
     5821 cggcggatgc acgcaccaaa ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa
     5881 tccagatccc ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg
     5941 aacaaatcaa ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagaac
     6001 aggcacgtaa aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa
     6061 acacaatgga gtgaatatga gcgtattacg ttcgttatta actgccgggg tgctggcgtc
     6121 gggcctgttg tggagcctga acggaattac cgctacccct gcggcgcagg catctgatga
     6181 tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataagcc
     6241 agataccgaa ggtatgcatg gaaaacatgc ttccgttatc aacccgaata acaaactgcc
     6301 ggtcacctgc accaactgtc acggccagcc atcaccgcaa caccgcgaag gggtgaaaga
     6361 tgtgatgcgc tttaacgagc cgatgtacaa ggttggagag cagaacagcg tctgtatgtc
     6421 ctgtcatctg ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa
     6481 agtggcgtgc gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag
     6541 tgacaaagga cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa
     6601 ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag
     6661 tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg
     6721 aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc
     6781 ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg
     6841 cgcctgacga ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc
     6901 ttccgtaagt cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt
     6961 gcgtcttttc gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt
     7021 tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag
     7081 acggcggaca aatgcgattt ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg
     7141 tgcgttgagg cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt
     7201 gagatttcgc aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc
     7261 aaaccgaagc tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt
     7321 ccgcatttca ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga
     7381 ttggtatttc tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg
     7441 cgggcggtgc agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga
     7501 tcctcggtct gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga
     7561 tgttccacta cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca
     7621 tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac
     7681 aacgctggtt gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc
     7741 atcgcggact ggaaacattg atgctggtgt tggcggtgtt gttgggggct tataccggct
     7801 ttctgctgtc ggcgctgaaa tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt
     7861 tcctcttctc cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc
     7921 aacgcagtaa cccgcattcc acggaagcgc agtttgtaca ccgtatggaa atccccgtgg
     7981 tatggggtga aatcttcctg ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg
     8041 gtaaagtgcg tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc
     8101 ttggtgtcgc cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca
     8161 gttccggcat tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc
     8221 tgcgcttttt cattctctac gccgggcagt taacggtggc gtaagccaga aaagaggtgg
     8281 tttctggacg tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc
     8341 aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg
     8401 cgactcactt gcatcggcat tctggcgcag ttcgcgctcc tgttgctcgc ctttggcgta
     8461 ctgacgtatt gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac
     8521 agcctgctgt cgtgggaact caagctggca gcggtgtggg gcggccatga aggttcgctg
     8581 ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag
     8641 caaaccgatc cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg
     8701 ctactgtttg tagtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa
     8761 ggccgcgatc tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt
     8821 taccttggct atggcggttt gatggtggcg gcgagcgtgg cgctggcgag tttactgcgc
     8881 ggcgagtttg atggtgcctg cgccagaatt tgttggcgct gggcactacc tggctggagc
     8941 gcattaacgg cggggatcat cctcggttcc tggtgggcct attgcgaact gggctggggc
     9001 ggctggtggt tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact
     9061 gcgctgctgc acagtttgtc cctgacacgc cagcgggaga ttttccgcca ctggtcgctg
     9121 ttgctggcga tagtaactct gatgctgtcg ctactgggta ccttaattgt ccgttctggc
     9181 attctggttt cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc
     9241 ctgtttgcac tgattagcct tgcgtctctg gctctgtatg gctggcgagc gcgggacggt
     9301 ggcccggtgg tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg
     9361 ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggt
     9421 ctgctgggct ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg
     9481 tttggcctgt tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg
     9541 cagcttccgg cgctggtagc tcatgcgggc gtgctgttat ttgccgctgg gatcgtggtt
     9601 tccagcgtca gccgtcagga gatcagcctg aatttacagc cgggtcagca ggtgacgctg
     9661 gcaggataca ccttccgttt tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc
     9721 gaaaaagcga tagtggcact gtttgaccat cagcaacgca ttggtgaact gatgccggag
     9781 cggcgttttt acgaagcacg tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc
     9841 atccatgact ggtatgcggt catgggtgaa aaaaccggag cggatcgtta cgcttttcgc
     9901 ttgtatgtac aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc
     9961 gcattattaa gcggatggcg ggggaggaag cgcgatgaat aaaggacttc tcacgctact
    10021 gctgttattt acctgttttg cgcgcgctca ggttgtagac acctggcaat tcgccaatcc
    10081 gcaacaacag caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa
    10141 tcagaactta ctggaatcca atgcaccggt ggcggtcagt atgcgccatc aggtttacag
    10201 catggtggcg gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg
    10261 agattttgtt cgctataacc caccgttaac gggtcagacg ctggtgttat gggcgctgcc
    10321 agtggtgttg ttactgctga tggcactgat cctctggcga gtgagggcga agcggtgaaa
    10381 cagtcacaaa taccggtgaa aatgctgaca acactcacga ttttgatggt atttctctgt
    10441 gtcggcagtt atctgttaag cccaaaatgg caggcggtac gtgcgcaata tcagcgtcag
    10501 cgcgatccgc tacatcagtt tgccagccag caaaccccgg aagcgcagct tcaggtattg
    10561 caggataaaa tccgcgctaa tccacaaaac agcgaacagt gggcgttact gggcgagtac
    10621 tatctgtggc aaaacgatta cagtaattcg ctgctggcgt atcgtcaggc gttgcaacta
    10681 cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc
    10741 cagcatatga ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac
    10801 gaaatcaccg ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa
    10861 gccatcgaac tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag
    10921 ctggtcgagt cgattaatat ggcgaaattg ttgcagcgga gatcggatta atcatctggt
    10981 gatgaagatt atgttattga gctgcataaa aaataatcga atgaacatat gccaaaaata
    11041 atcaccaatc agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta
    11101 ttcattaatc ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg
    11161 ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta
    11221 caaaccaacg caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg
    11281 gggaacggcg ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct
    11341 ggcagattct gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata
    11401 gcgacagccg cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc
    11461 atctgattaa aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg
    11521 gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa aaccattatc tacttcgagg
    11581 tgatcaccac catcgctatc attttgggga tcactctggc gaacgtcttc cagcccggtg
    11641 ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg
    11701 aagcggtaca aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga
    11761 acattgtggc gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt
    11821 ttggtctggg gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc
    11881 gctccatctc tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg
    11941 gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg tttctcttct ctgtggccac
    12001 tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg
    12061 gaattgtggc gcgcctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg
    12121 agctgattct ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga
    12181 agatggaagc ctacggagca ccggcgtcga tcaccagctt cgtggtgccg atcggttact
    12241 cttttaacct tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc
    12301 tgtatggcat tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga
    12361 cctcgaaagg gattgctggc gtgccgggcg tgtcgtttgt ggtgttgctg gcaacgctgg
    12421 gtagcgtagg tattccgctg gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg
    12481 acatggcgcg tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca
    12541 agtgggaaca caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca
    12601 aatttgataa aactgcggat caataattga agattgccgg ggttatccac ccggcaatgt
    12661 gtggatgcct gatgcgacgc tggtgcgtct tatcaggcct accttaaccg ttggttaatt
    12721 tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca
    12781 gcgccttttg tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg
    12841 cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg
    12901 ccttgtcatc gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt
    12961 gggcatcgac ggcagagtcg ttctccagat cttccgaggc gttttctaac agcgaaatcg
    13021 cctttggata atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt
    13081 caccttcttt actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt
    13141 ccagactgac cggattggtg attttgatct gcgccagcag cgcgcaggca tcagcatcac
    13201 cgctgtcggc ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc
    13261 agctgtcggc aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact
    13321 ggctgccagg ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga
    13381 taattttttt cattttgata atacgattgg gtagggagta accagtataa agagttcgaa
    13441 tggataaaaa aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg
    13501 cttcgcgcag gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc
    13561 gctgatcggc gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa
    13621 tcgggcttaa gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca
    13681 tgtaaatcgc ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt
    13741 tacgcgagtg cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg
    13801 tattgatttg tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg
    13861 tcatcgaacg gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact
    13921 cgtcggtgag tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt
    13981 tcggggtatc aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat
    14041 cgcggcaagg ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga
    14101 aatccgggca cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg
    14161 gataacccat acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt
    14221 tcggttcaac tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt
    14281 catgctcgcc ccacgacgtt gacggtaaaa taacatccgc cgccgacgcg gttttggtca
    14341 taaagatgtc ctgaacgata accagttcca gatcttcaaa ggctttacgt actgccgaca
    14401 gctccgcgtc agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat
    14461 gcgctgcgcg gtgcggcagc tcgctgatgc gataaccggt atgagcaggc aggctttcca
    14521 cgccccaggc tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg
    14581 gatacgtatc cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa
    14641 ccgggtttac acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca
    14701 gagaacgcac ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg
    14761 cggcgctttt cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc
    14821 cggtgatatc ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa
    14881 agccttctgt acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat
    14941 ggcccatcgc attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag
    15001 caatgcgcgc ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt
    15061 tagcgttaat tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa
    15121 cgaacactaa atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga
    15181 ccgattggtg cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt
    15241 tattggtacc aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg
    15301 taccacgcga ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc
    15361 tcaggcgctc ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc
    15421 cacgctggcg acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa
    15481 tgaaatccca gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg
    15541 ctgcctccgc ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg
    15601 cgcaataggg gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca
    15661 cgcatacgcg ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt
    15721 ccctgatatt acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt
    15781 ttgacgtgac gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt
    15841 ctcgacgacg ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg
    15901 gatcacccgg cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg
    15961 ggcttcagtg gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg
    16021 ctgggtgaag cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt
    16081 ttgcagacgc gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta
    16141 acgttcaatc atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc
    16201 accgtcaaac agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt
    16261 gaataaggta tccaggtgga tggcatcaaa gccgaaaaac gctttgatat caaagctcgg
    16321 gtagaacagc gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat
    16381 ggcttgcaga tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt
    16441 ctggactcgt ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat
    16501 caatgcacgc agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt
    16561 atcgaccgcc agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg
    16621 cgccacttta ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa
    16681 cagatcgagc atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt
    16741 cagcgatagc tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc
    16801 ggcaaccgct gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac
    16861 tgtggcttcc gtatagtacg gcccgtccat acccagtgcg ggcgcatcca tcgcataagg
    16921 gcttaaaaag ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg
    16981 taattgtgaa ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt
    17041 cgcttcggcg agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg
    17101 gtcattgagt tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc
    17161 gtcggccagt ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac
    17221 cagggcacag ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga
    17281 aagttgacga ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc
    17341 ggggctgtcc agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat
    17401 aaggtgggca ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg
    17461 ctgagtgcgg ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct
    17521 ctgtccggct gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg
    17581 tgaagaatat cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt
    17641 tcttcgctgt ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta
    17701 aacgccgcat gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc
    17761 tggcgtggga tccgcattac tttacttaac atacccagcg tgccagccag tttttgcggc
    17821 agtgtgcgcg cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta
    17881 ccaatgatga tacccatggc gcgatcgcga atttccacca ggtcgtacac cgggccaaaa
    17941 acgttttcga gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag
    18001 cgttcagagc tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc
    18061 aattcgacaa tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc
    18121 gcgccgcaaa aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt
    18181 gggttagcga cgatcacgca tgtcagcata caggtgtgaa tgccttccca gtccacgccg
    18241 ctgtagaagg tgtaacagat caaacaggcg agcagtgttt ttaccgcgta gcgcatatag
    18301 tctggattgg taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc
    18361 gtattcgggt ccatctgacc cagttgtaac aacgtctggc agatattctc caggttacat
    18421 tcccgtgccg ccatcgcttc actttcagtg atccgccagt cgctttgcca gcactgacct
    18481 tcagcaacgg catgctgcag cttattgatt tctgaggcta atttttgccg aaattcaata
    18541 attgcctgag aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag
    18601 gttaccgttg ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc
    18661 gcgagacaaa agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg
    18721 ggtagcggtg cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc
    18781 gcctgatgca tttgcgtaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc
    18841 agcaaggttg gataaaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc
    18901 ggatagtcga gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacggcgaaa
    18961 aagaccagtc ccagccgatg ggtgcgcatc aaaaacatac agcccatcag gatcggcccg
    19021 gcgatgacca atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg
    19081 ccgatctcca gcatcgtggc gaccacaaat aagatcgcga caaatttggt gtaaaacgcg
    19141 ttcgactgaa taccgtaaaa cagcactgcc agcgataacg ccacgaaagg gatctcaaag
    19201 gtcatcgaga tcagaatcac cagcaggcag cctacccaga gttgcacggt ctgcggcacg
    19261 cggccgggtc gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt
    19321 aatggcaggg agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc
    19381 cgatgcggaa catttccggg tcaggtttat caaccatgat tttgaccgga aaacgctggg
    19441 caacgcggac ccagttaata gaacgagaaa ctttcggcag gccgcccagc accaggccgc
    19501 cgtcatccgg tagcacgccg tagccaatcg aatccacttt accctcgaag gttttgccgc
    19561 tgtcactcat caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt
    19621 cgcggaagtt ggcgatcacg taccagtgac gagtgtcgat tagagtaaaa atagggcgca
    19681 tggcagaagc aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg
    19741 cgcgaacggt cgccatttcc agatgaagtt tggtcagggc aatatccgct tcgaccgccg
    19801 cacgttgcgc caccagcgca tcgacgccgc tgacggcgct ggcggctgac tgcgcctgta
    19861 acaatacggc attaagatcg gcttctgcgg cgcgctgcgc ggtcctcgca cggtcaacgt
    19921 cttccgctga gacaaaacct tcttttagca acggctcggt acggcgtaat gtatctgtgg
    19981 cctgtttcgc ggcggcacgg gctttttcta ccgtggcatt aaccgagtcg gcaccaaact
    20041 gttgcgcatc aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg
    20101 cttcagcttt cgccagattg gctttgtacg ggcgcgggtc aatgcggaac agcaaatcgc
    20161 cctgtttgac tgcctggttg tcggtgaccg ccagttctac aatgcgaccg ctgacttccg
    20221 gcaccacatc aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta
    20281 cgcgccagat aacgaaaaca agggcaacca gcgccaacgc aaccactaac agagcaggga
    20341 atttactgcg aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga
    20401 aaaatgccag ccagaacagc atggcgtaga gagcaaaaag ggcggtataa ataattgcga
    20461 caaatgccag attgatattt gcccgctgaa taactcgcct cgtgatgagc gtcaaaataa
    20521 ggctggcaat ggcgcagaaa aaccagctgg gataataagc accgatcacc gggattgccg
    20581 gagataatga gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt
    20641 gcatcgccat gcgagagaga actgttggca tattaaatac atcctgtctg ctgcgaacaa
    20701 tatctgtagt gaatatcaat atccattgaa ggcgtcagta tggctatttt cttgatgaat
    20761 aaaaatagct ttatacagcc accgcattca taacttatac attttattta ttaattggct
    20821 gtatgtattt ttaaaataaa tacttttggg ggcttcagcg cccccaaaag cgagtgacat
    20881 taatttgggg ttacgatatt cacccacaaa tcgaaattat ccagacaggc gataatttct
    20941 tccagtttcg cgccattgcc aataattttg gctttcttcg cttttaccag atccgccatt
    21001 ttggcttgcc cggtcagcac ggcgtgcaga tcttcacggc tgatgtagaa agaggcgtca
    21061 gcttgggatt gcagtgtttt acggtagtta agcacgctat cgttcagcgt caggttgagg
    21121 ttatcgccat tgctcatatt gaagttcagg ctgatatttt tacccgcggc tttcgcgcta
    21181 tcgaggcgaa cggacataaa gtcgaacagc atttctaccg acatcccgcg aatggtgtcc
    21241 ggggaaccgg tggtgccgtg gctgaactta tgcacccctt cgcgcagctc tttcgcgccg
    21301 gtcaggtaga aaccgcgcca ggtggcggac tcggcctgat agcccagctg ttcaaagtta
    21361 ttcgcttgca gattcttcgc gacctggtca cccgggttgg cggcaatcac ctgtttcagc
    21421 agttctgccg accagcggta atcaccttgc ttgttcgctt cttgcgccag gttgatgaca
    21481 cgggcagaac cgcccagcgc ctgcacgtaa cgtttaccca tctccacctg accatacgga
    21541 tgcaggttag ccgggttacc gtcgtaatag ccaagataga agttatacac cgcgcgggcg
    21601 ttgtggctga cagaaccgta atagccacgg ctggcccagt tattggcaag tgcaggcggc
    21661 agtttaatca tgtcgccgat ttcattcatg gtgtagccct ggttcgccag gtgcagggtc
    21721 tggtcgtgaa tgtacttgat agtatcgcga tatttaccaa tataatcatt gatatgctta
    21781 ttgccccaga ccggccaggt gtgcggcata aacaggactt ccgcgtcgtt accccacata
    21841 tccagcgttt cgttcagata ctcggtccac ttgctggtgt cgcgggtttt cgcgccgcgt
    21901 agagtgtaga agttgtgcag ggtatgcgtg gcgttctcgg cggtacacag cgctttcagg
    21961 gccggaatat agaagtgcat ttcggctggt gcttcgctac ctggggtcat caggaagtca
    22021 aactccaggc cgtcgataat catcttctca ccggttttgg tgatggtttt gttcggtgcg
    22081 atgatgctcg ggtagcccga tgccagcgtt acgccaagac cattgccgat gttaccctga
    22141 gggttatgcg ccagcagcag gccgtaggag taaagtgcac ggcggctcat aatattaccc
    22201 gccagtacgt tttcgctgat ggcttcgtcc ataaagccag caggggcgat aacctgcact
    22261 ttaccggatt tcacatcggc ttcagagata atgcctttca cgccgccata gtggtcggta
    22321 tggctgtggg tataaataac cgcaacaatc ggtttttgcg ggcgattttg gaagtaaagg
    22381 tcaagggcgg cttttgcgct cggtggcgtc accagcgggt cgatgacaat aatcccggta
    22441 tcaccttcga taaaggtgat gttggagata tcctgaccgc gaacctgata cattctgtcg
    22501 gtgactttaa acaggccgga aataccgttt aactgcgact gacgccacag gctagggtta
    22561 acggtttcgg gcgcgggcgc attgatatca aatttataat cttccccgcg ataatatggt
    22621 ttgccattag catcgcgcag aataccttca tccagcagcg gagcgataaa tccacgctga
    22681 gcgtcgtcaa aatcctgacg atcgctaaac ggtaatgatt tggcgtaatt ctgattaacc
    22741 tgttgggtat attgagtggc ggctttcgct tcatctgttg cgtaagcggt atttaccatc
    22801 atgccgctgg cggcagttaa cgcaataata attctgctca aacggaataa ccgagagtta
    22861 ttcatataaa tctccattta tcatttatgt cgtaaatatg taatccatgc aaaatacgtc
    22921 atttaaagct ggggttatca taattgggaa tttgcgactt taataagtgg aagtgtgagc
    22981 tgaacgatcc attttattag gcatttgtgc tgaagaggaa aagttacagt aaaactatca
    23041 ttgcagaaaa taatatgagc cgttttatgt gtttattata tatattgtac ctgtcgttgg
    23101 cacagcggga cattgcgcta ctgtgccaac gctttactca tggggcttta gcacaggact
    23161 gtggcaaaaa atgttgatgc gccaatattg ctgcaccctg agcgccatta aagtcagaag
    23221 atgaggcggc aataaagcgc acgacctgat gcggcagtgg acggcgcagg tacttttggg
    23281 tcatggcaat gagagtctcg cgtgggaagg cgggcatatc catcacgcca ccgcccagaa
    23341 tcaccgcatc gggatcgaac agattaatgc tggtggcaat ggcccgtgcc gcgttttcaa
    23401 gcagactctg gacgaaaggg gcgttttccg catggacgaa aaggtcgctc aatgggtaat
    23461 ttcggggctg ttgttcgtac cagcgtctta gcgccattcc agagcaattg gtttccaggc
    23521 acccaggatt gccacacgcg cagtgttggg tcatatctcc cagggggata tgacccagtt
    23581 cgcctgccac accgtgtgca cccgtccacg gcgcaccgtt catccacact gcgaacccca
    23641 tccccgtacc gagataggcc gccagaacca gttgttgcgt aaggcggttt tctactacgt
    23701 cccaggagag ttgcaggtta acgtcgcggg aaaactcaac cggacaattc agcgtatttt
    23761 cgagcttatc ggcgagatca tataaatccg ccgctgttaa cggcaggtta ggcgtagaaa
    23821 ttatggtgcg tttatcttta ctgaccagcg ccggaaatcc catcaccaga ccatgacagc
    23881 gagcgttaaa gcgcctgagt tgctcgtcaa tcatttcgcc gatacccgac accaggccgg
    23941 gagcaatgac ttctgcggtc cgcttttttt cgcagtgtag cgtttcacct tctgctgtcc
    24001 gcagacaaaa gcggatatgc gttgccccca tatccacgcc cgctacgacg ttatgctgtt
    24061 tttgcatgag gctgtacctc gctttttgca gccagaatct gcgtggtcat aattctccat
    24121 gcttcgtcga tattttccgc atgattaaac aggccggaag tgccgacgat aaagacatcc
    24181 gcccctgccg ccatcagttt ttcgtaagtt gcctggttgc aggaaccgtc cacctcaatt
    24241 tcgtactcca gaccttctcg ttcacgccat gccttcagtt cggcaagttt atccagcatt
    24301 tcaggaatga acggttgtcc ggcaaagccg ggatcgacag tcatgaccgt aattttatcg
    24361 gccttatgga tatagtattt catggcctca actggtgtct ccgggttaag gatcagcccc
    24421 actttcatgt catgacagcg gatttcatca atcaggcgga acgcctgacc gttgatagtt
    24481 tccggatgca aagtgatgaa atcagctccc gcacgcgcca gttgagcaat gtaatcctgc
    24541 ggccgcgtca ccatcagatg acagtcgagc ggtttagttg ccagtttttt aacctgactt
    24601 acgaagaacg gtgagagtgt cagattgggg acaaagtgac catccatgat atcgatgtga
    24661 aagtaatcgg catggctgtc gataaattcg atctgttctt taaatttcag cagatccata
    24721 cacattaacg agggggagat tttcatgttc aattccttac ttactgataa gacggtcaag
    24781 ggcgacagcc gcgataatta atccgcccat caccaccagt tggtaatagg tttgtacctg
    24841 caaaatattc agaccgttgt tgatggtgcc gatgatcaac ccgccaatca ccacagagaa
    24901 aatgcgcccc ttgccgccga agaaactggt gccgccaatg atggcgctgg caatggcata
    24961 ggtttcaaaa cccataccgg caagcggttc tgcggcaccg agtcgtgcag ttgagacgac
    25021 gcctgccaga cctgcacaaa caccggagat gataaacacc accaggatgt ggaatttcac
    25081 gtcaatcccg gaatagaacg ccgaattttt gttgccgccc agtgcgtaga tgttgcgccc
    25141 gagccgcata cgcgttgtca gaaaccaaag gatgagcgcg acaattagcg agaagataac
    25201 ggggacaggt atcccaatta cgctggcggc aaagaagttc acgaagtcaa aggagaagcc
    25261 gtataccgag ttggcatcgg agatcaccag cgtgatccca cggaaaatcg cgttggtgcc
    25321 aagggtgatg atgaacgggt gtagccccgt ccagttgacc aggcagccgt tgatcgcccc
    25381 cagtgcgccg ccaaccagta caccgccaat catcgctgcg agaaacgggt caacacctgc
    25441 caacatcagt ttggcggtca ccataccgga aagcgccaga atcgcgccaa ccgagaggtc
    25501 gataccagcg accaggatag cgaaaaactc gcccatgccg atcaataccg tcacggagct
    25561 ttgaacaaaa atctgggtaa tattattggt ggtcagaaaa tattctggcg acagcgaacc
    25621 aaaaatggcg acgatgatcg ccaggataaa aaaggtgccg tatttatccc agaacagcgc
    25681 aaagttgaac ggtttcttct cgctcgcttc gctttttact cttgtggtaa agcccatgcc
    25741 ataatctcct cttcgctcat gtcatcgcga ttcgtcagga tttgcgtcag tcgtccttcg
    25801 cagaacacgg cgatgcggtc gcagacggtg ataatttcag gtagttcaga tgacaccatc
    25861 aggatgactt ttccgtcgtc cgccagttgg cgcatcactt tgtaaatttc ggctttcgcg
    25921 ccaacgtcga tgccgcgggt aggttcatcg aaaataatca cttccgggca acagcacagc
    25981 catttggaga tcaggacttt ctgctgattt cccccggaga gtttggtgat attctggttt
    26041 accgaatgac atttcagcgc cagcagttcg cgttgatttt cagcggtacg ttgctcgtca
    26101 acttcatgaa acaagcccat cgcgccttta tagccgccgt ctttcagact gcggctgatc
    26161 gccatgttct gagcgatgga aaagttggga aaaaaaccgt tatcccggcg gctttcagtg
    26221 atgtaagcca tccctttttt cactgcatcc aggggggaac gtggagagat atctttgcca
    26281 ttaagacgga tttctccgcc agcgcgttta tcaacgccaa acagacaatt catcagttca
    26341 gtacgtccgg aaccgaccag cccggcaaag cctaatattt ctccccggca gacgctaaat
    26401 gagatatccc ggaccttttt tctgtcacgg ctggtgacgt tgcgcacctc aaaaaccgta
    26461 tcgtgcacaa ggttgctgac attctccttc atcgcgttaa aacggttttg cagttcgcgg
    26521 cctaccatca gacggacgat atcgtcattt gacacatcgc ttaccatgcc gctgcaaacg
    26581 ctgctgccgt ctttcatcac cgtatagcgg tcgcaaatac ggcgaatttc cgccaactta
    26641 tgcgagatat agacgatggc cgtaccctct ttacgcaact gattcatgat cagaaacaga
    26701 tagtccacct ctttattggt gagtgaggag gtgggttcat ccatgatgat gactttggca
    26761 tcgagcatca gcgttttggc aatttccagc atctgcttgt ggctgataga tagattcgcc
    26821 actttctcat ctaaatcaac tttcaagccc acgcgtaata acatcatggc ggcgcggaca
    26881 cgcatttctc gccagtcgat aatattgacg ccacagattt ttttcgtcag atgacgacca
    26941 atatataaat tttccagtac ggttaattca tcaataacgc tgagttcctg ataaataatc
    27001 ccgataccga gttgtgccgc taatttatga tccagcttgt tatagctaat gttattaatg
    27061 gtaatggtgc ctttggtcgg ctcatgtatt ccggataaaa ccttcattag cgtggattta
    27121 cccgcgccat tttctcctag taatgcatgt atttcaccag gataaaccgt taaattaacc
    27181 gactttaatg cgtgaaccgg accaaaggac ttgccgatcc ccgccatcga tatatatggc
    27241 gtggccataa ataacctctc tgcacgaaat atcgtattat tgcctgatgg ctacatttat
    27301 caggtctacg ttcaggttat tttgttggcc ggataagaca cttcattagc atcatcatcc
    27361 ggcatcattc agttttattg agtgaccagg attgaatcga ccagtttaaa ttccggtgct
    27421 ttatccagcg ggataacctt gccggatttc tcagcgttaa ccatcagctt cagacccgtt
    27481 gcgccaatat ctgccgggtt ctgcgcaacc gtcgcggtca tttgtccggc ttccaccatt
    27541 ttgcgggctt ccggaattcc atctgtaccg acgaccaata ttttgcccgt ttttccggcg
    27601 tttgcgactg cctgagcaac acccattgcc atcgtgtcat tcgcgcaata gatcgcttta
    27661 atattcggat tacgttgcaa cacgttagtg gcgacatcca gtgctttaat gcggtcccag
    27721 tcagcgggct ggctggcgac aagcttgatc tggcttgctt ttttgaaggc ttcggtggca
    27781 ccattacgac gcgcttcacc ggaggcgtta ccggctttac cctcaatgat tgcgacttca
    27841 ccaccttcag cgcccaattt gtcaataatg aacgacgcgc ctttcgcccc gacagcaacg
    27901 ttatcggtgg tgacaaaagc ttccacattg ccgccagctt ttttcagatt atccatgtcg
    27961 attttttcat cgagattaac cagataaatg ccttttttcc atgcgcgggc gacaggcatg
    28021 accagattca ctgaggataa tggagcgaag gcgatacctt tgtaattttt attactgaga
    28081 tcttcaaata actgcaattg agattgaaaa tcgccttctg aaggagaggc aaaaatatca
    28141 acgctgacgc ccagtgtttt tgcttcatct tcaatgcctt ttttcatatc tacccaaaat
    28201 gggttggaga gggttttcaa tacgacagca tattcg
//
LOCUS       DAPPNJ010000042        28044 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000042 DAPPNJ010000000
VERSION     DAPPNJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.042"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..808
                     /locus_tag="RPM73_001463"
     CDS             <1..808
                     /locus_tag="RPM73_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX6519837.1"
                     /translation="ESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRI
                     MSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANH
                     HGITFDEQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISC
                     RSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDS
                     KHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1174..2241
                     /locus_tag="RPM73_001464"
     CDS             1174..2241
                     /locus_tag="RPM73_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX6519838.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2238..4973
                     /gene="rbbA"
                     /locus_tag="RPM73_001465"
     CDS             2238..4973
                     /gene="rbbA"
                     /locus_tag="RPM73_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDX6519839.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4973..6097
                     /locus_tag="RPM73_001466"
     CDS             4973..6097
                     /locus_tag="RPM73_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6519840.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6156..6431
                     /locus_tag="RPM73_001467"
                     /pseudo
     CDS             6156..6431
                     /locus_tag="RPM73_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6428..6787
                     /locus_tag="RPM73_001468"
     CDS             6428..6787
                     /locus_tag="RPM73_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDX6519841.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6907..7308)
                     /gene="nikR"
                     /locus_tag="RPM73_001469"
     CDS             complement(6907..7308)
                     /gene="nikR"
                     /locus_tag="RPM73_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDX6519842.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7314..8120)
                     /gene="nikE"
                     /locus_tag="RPM73_001470"
     CDS             complement(7314..8120)
                     /gene="nikE"
                     /locus_tag="RPM73_001470"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDX6519843.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8117..8881)
                     /gene="nikD"
                     /locus_tag="RPM73_001471"
     CDS             complement(8117..8881)
                     /gene="nikD"
                     /locus_tag="RPM73_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDX6519844.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8881..9714)
                     /gene="nikC"
                     /locus_tag="RPM73_001472"
     CDS             complement(8881..9714)
                     /gene="nikC"
                     /locus_tag="RPM73_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDX6519845.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9711..10655)
                     /gene="nikB"
                     /locus_tag="RPM73_001473"
     CDS             complement(9711..10655)
                     /gene="nikB"
                     /locus_tag="RPM73_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDX6519846.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10655..12229)
                     /gene="nikA"
                     /locus_tag="RPM73_001474"
     CDS             complement(10655..12229)
                     /gene="nikA"
                     /locus_tag="RPM73_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDX6519847.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12340..12927)
                     /gene="acpT"
                     /locus_tag="RPM73_001475"
     CDS             complement(12340..12927)
                     /gene="acpT"
                     /locus_tag="RPM73_001475"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDX6519848.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12982..14031)
                     /locus_tag="RPM73_001476"
     CDS             complement(12982..14031)
                     /locus_tag="RPM73_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDX6519849.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14163..15380
                     /locus_tag="RPM73_001477"
     CDS             14163..15380
                     /locus_tag="RPM73_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519850.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15384..15941)
                     /gene="dcrB"
                     /locus_tag="RPM73_001478"
     CDS             complement(15384..15941)
                     /gene="dcrB"
                     /locus_tag="RPM73_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDX6519851.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16014..16679)
                     /locus_tag="RPM73_001479"
     CDS             complement(16014..16679)
                     /locus_tag="RPM73_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDX6519852.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16900..17145
                     /gene="tusA"
                     /locus_tag="RPM73_001480"
     CDS             16900..17145
                     /gene="tusA"
                     /locus_tag="RPM73_001480"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDX6519853.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17247..19445)
                     /gene="zntA"
                     /locus_tag="RPM73_001481"
     CDS             complement(17247..19445)
                     /gene="zntA"
                     /locus_tag="RPM73_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDX6519854.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19519..20145)
                     /locus_tag="RPM73_001482"
     CDS             complement(19519..20145)
                     /locus_tag="RPM73_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDX6519855.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20286..20645
                     /locus_tag="RPM73_001483"
     CDS             20286..20645
                     /locus_tag="RPM73_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDX6519856.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20648..20917)
                     /locus_tag="RPM73_001484"
     CDS             complement(20648..20917)
                     /locus_tag="RPM73_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDX6519857.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20907..21503)
                     /gene="rsmD"
                     /locus_tag="RPM73_001485"
     CDS             complement(20907..21503)
                     /gene="rsmD"
                     /locus_tag="RPM73_001485"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDX6519858.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21653..23146
                     /gene="ftsY"
                     /locus_tag="RPM73_001486"
     CDS             21653..23146
                     /gene="ftsY"
                     /locus_tag="RPM73_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDX6519859.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23149..23817
                     /gene="ftsE"
                     /locus_tag="RPM73_001487"
     CDS             23149..23817
                     /gene="ftsE"
                     /locus_tag="RPM73_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDX6519860.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23810..24868
                     /gene="ftsX"
                     /locus_tag="RPM73_001488"
     CDS             23810..24868
                     /gene="ftsX"
                     /locus_tag="RPM73_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDX6519861.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25113..25967
                     /gene="rpoH"
                     /locus_tag="RPM73_001489"
     CDS             25113..25967
                     /gene="rpoH"
                     /locus_tag="RPM73_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDX6519862.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26238..27341
                     /gene="livJ"
                     /locus_tag="RPM73_001490"
     CDS             26238..27341
                     /gene="livJ"
                     /locus_tag="RPM73_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDX6519863.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27540..27923)
                     /gene="panM"
                     /locus_tag="RPM73_001491"
     CDS             complement(27540..27923)
                     /gene="panM"
                     /locus_tag="RPM73_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDX6519864.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6653 a         7281 c         7773 g         6337 t
ORIGIN      
        1 agaaagtctt cagccttatt tagttattaa taaagacgct gtgacgccat gtacgtttct
       61 tggcgaccaa accggcgatc gatttagtaa tatctgtggc gatcaattta tcatcgattt
      121 gttaaaacgc ataatgagta tcaatgaaaa tgttcatgta ttagctggaa atcatgaaac
      181 gaattgcaat ggtaactata tgcagaactt cacgcgtatg aaaccacttg atgaggatac
      241 ctacgctggg ataaaagatt atcctgtatg tttctatgac cccaaataca aaataatggc
      301 aaatcatcat gggataactt ttgatgagca gcgaaaacgt tacattatag gaccgataac
      361 agtctctatt gatgaaatga ccaatgctct cgatccggtc gaattagcgg caattatcaa
      421 taaaaagcat catgctataa ttaatggcaa aaagtttaaa actagccggg ctatctcttg
      481 tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac ttgaagcact
      541 attagcatgt tcacaaatgt taggtataaa tcaagttgtt gcacataacg gaaatggtgg
      601 aagagaacgc ataggcgaaa cggggacagt tttaggtctt aacgcccgtg acagtaaaca
      661 tgctggaaga atgttcagca tgcataattg ccaaattaac cctggtgcag ggcctgagat
      721 aaccactccc tggaagtctt accagcatga aaaaaacaga aatggactta tgcctttgat
      781 tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta atgttactat
      841 ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc
      901 gtgaaacgct atccaacagg atggataccg ttttgtcagc cagttttata gatcacactt
      961 atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt
     1021 tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta tttgccgttt
     1081 ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg
     1141 tcgtttgctt acagggatta tggcgggtcg attatggata agagtaagcg ccatctggcg
     1201 tggtgggttg tcgggttact ggcggtggcg gctatcgtgg cgtggtggct gttgcgcccg
     1261 gcaggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat
     1321 atcgccagca aaattgccgg gcgtattgac accattctgg tgaaagaagg ccagtttgtt
     1381 cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa
     1441 gccattgcgc aaatcaaaga agcacaaagc gccgttgctg ccgcgcaggc tttgctggag
     1501 caacgccaaa gtgaaactcg cgccgctcag tcgctggtta atcaacggca agctgaactg
     1561 gactccgtag ccaaacgtca tacccgttcc cgctcgctcg cccatcgtgg ggctatttct
     1621 gcgcaacagt tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg
     1681 gcaaaagctc aggtttcggc ttctaaagcc gctatagaag cggcacgcac caatatcatt
     1741 caggcgcaaa ctcgcgtcga agccgcacaa gccactgaac ggcgcattgc cgcagatatc
     1801 gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca
     1861 ggcgaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat
     1921 atgactttct tcctgccaac cgaacaggcg ggcacgctga aactgggcgg tgaagcccgg
     1981 ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa ccatcagttt tgtcgccagt
     2041 gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa actgatgttc
     2101 cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc
     2161 ggtttaccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc
     2221 gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac
     2281 tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc acactcgata
     2341 ttccggcccg ttgcatggtc gggctgattg gcccggacgg cgtcgggaag tcgagcttgt
     2401 tgtcgttgat ttccggtgcc cgcgtcattg aacagggcaa tgtgatggtg ctgggcggcg
     2461 atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg
     2521 ggctgggcaa aaatctctac cacaccttat cggtgtatga aaatgtcgat tttttcgctc
     2581 gcttgttcgg tcacgataaa gcggagcggg aagtccgaat taatgagctg ctgaccagca
     2641 ccgggttagc accgtttcgc gatcgtccgg caggtaaact ctccggcggg atgaagcaaa
     2701 aactggggct gtgctgcgcg ttaatccacg acccggaact gttgatcctt gatgagccaa
     2761 caacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac agtattcgcc
     2821 agcggcagag caatatgagc gtgctggtcg ccaccgccta tatggaagag gccgaacgct
     2881 tcgactggct ggtagcgatg aatgccggag aagtgctggc aactggcagc gccgaagagc
     2941 tgcggcagca aacgcaaagc gccacgctgg aagaggcgtt tataaatctg ttaccgcaag
     3001 cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aatgcagaga
     3061 ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg
     3121 tcaatttccg cattccacgc ggggagattt ttggttttct tggatcgaac ggctgcggta
     3181 aatccaccac catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc
     3241 tgtttgggca accggttgat ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt
     3301 cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaaccttgag ttacatgccc
     3361 gtttgtttca catcccggaa gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt
     3421 ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt gccgctcggc attcgccagc
     3481 ggctttcgct ggcggtggcg gtgattcatc gcccggagat gttaatcctc gatgaaccta
     3541 cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtc gatctctcgc
     3601 gccaggacaa agtaaccatt tttatctcca cccactttat gaacgaagcg gaacgttgcg
     3661 accgcatctc actgatgcac gccggaaaag tgctcgccag cggtacaccg caggaactgg
     3721 ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg caggaagcgg
     3781 caggacagag caacgaagcc gaagcgccgc ccgtgataca cgacaccacc cacgcgccgc
     3841 gtcagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagca ctggaactgc
     3901 gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac agtgatcctg atgctgataa
     3961 tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc
     4021 agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac tttatcgaac
     4081 agccgccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatataacgg
     4141 tggcaattga gatccctccc aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac
     4201 tcggcgtctg gatcgacgga gcgatgccga gccgtgccga aacggtaaaa ggttacgtgc
     4261 aggcaatgca ccagagctgg ctacaggatg tggcgagccg acaatccaca cccgccagcc
     4321 aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta aaaagcctgc
     4381 cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg
     4441 cccttagcgt ggtgcgggaa aaagagcttg ggtcgattat caacctttac gtgaccccca
     4501 ccacgcgtag tgaatttttg cttggtaaac agttgccata catcgcgctg gggatgctga
     4561 actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt
     4621 tcctgacgct caccctggcg gcgctgctgt atatcatcat tgccaccgga atggggctgc
     4681 tgatctccac ctttatgaaa agccagattg ccgccatttt cggaacggcg attatcacgt
     4741 tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg gaagggcctg
     4801 gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcggaacgt
     4861 tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca
     4921 tcccgctggt gatgggctta agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca
     4981 tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc
     5041 gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac
     5101 gccaggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc
     5161 gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga tgatcaccgc
     5221 cgatgagatg gatgccggac tggacgccgg gcgttatacc tttgcgataa atattccgcc
     5281 taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga acgtcgatgc
     5341 cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga
     5401 agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac
     5461 ccggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcaatcat
     5521 caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga
     5581 acacggcacg gtggaacact tgttggtgat gccgataacg ccgtttgaga tcatgatggc
     5641 aaaggtctgg tcgatggggc tggtggtgct ggtggtgtcg ggattatcac tggtgctgat
     5701 ggtgaaaggc gtgctgggtg taccgattga aggctcgatc ccgctgttta tgctgggcgt
     5761 ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc
     5821 aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg
     5881 tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc
     5941 gacgacacac tttgttagcc tcgcgcaggc cattctctac cggggtgccg gattcgaaat
     6001 cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca ccatcgcgct
     6061 gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt gacttcatac
     6121 tgtgatgtca taaagtgaag tcataaggga atgtcatgta aacacaggta caggctttac
     6181 gaaagaaaca aaaaaacaca ctggatcagc tatttaaatc acctttacct caaggaatca
     6241 aatggactga tatagaatct ctggttaaag cgttaggcgg agaaattaag gaaggtaggg
     6301 ggtcgcgctg taagttcata ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt
     6361 cgccagatac cgataaaggc gctgtagaaa gcgtgcgtga ctggttacta agtatagggg
     6421 taaaaccatg atcaaactga agacgccaaa ctctatggag attgccgggc aacctgctgt
     6481 tatcacttac gtgccagaac tgaatgcatt tcgtggcaaa ttcctgggct tgtcaggtta
     6541 ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa gaaggggaac tttccctgcg
     6601 agagtatctt gaggattgta aagctgcggg tattgagccg tatgcgcgta cagaaaagat
     6661 taagaccttt acgttacgct atccagaatc gctgagtgaa cgccttaata atgcggctgc
     6721 gcagcaacaa gtctctgtta atacctatat tattgaaaca ctgaatgagc gtttgaatca
     6781 tttataaatt ttagcggcgt acattacgca gaatatctgc ctttacagga tctatctatg
     6841 caactgaact taattttata aattaagttc agttaatatc ttttctttcc tcgtttcaga
     6901 aataactcaa tcttccttcg gcaagcactg caaatgcccg tgccgcacgc cgcgctgggc
     6961 gataacgtca tcggcaaaat gctgcacgtc gcccatatca cctttcagca cggcgatttc
     7021 cagacagtcg tcgtgattga tatgcacatg cagcgtggcg acggagagat cgtggtgatg
     7081 gtgctgggtg gagacaatgc ggctggctaa gtcgcgtttt tcgtgttcat acacatagga
     7141 gagcaccgcg aaaccttgcg tgccgtgctg ctgagtggcc tcctgggcca gggcgctacg
     7201 cagaatatcg cggatagctt cggaacggtt gttataacca cgacgctggc tcaggctgtc
     7261 cagcgtctcc agtaaatcgt catcaagcgt gatggtgact cgttgcattt gcgttaaacc
     7321 ttttctgtgg tgcgacggcg cacggggaat gcgggtaata ccgcgttttg tagcacacgt
     7381 ccggcgtcag aggaaaaggt taatttatct cccaccacct gggtttcgac gatttgtccg
     7441 ttgtccatca ccatcacccg ctgacagaag cgttccacca ggcgtaagtc gtgggtgatg
     7501 aacaggcagg cggtgccaaa ctgttgttgt agctttttca gcaggcgaat gacgcccgcc
     7561 tgtaacacga gatcaaggtt agaaacggct tcatccagaa tcagtagttt cggttcgacc
     7621 gccagcgcgc gagccaggca gacgcgctgg agctggccgc cgcttaactg cggtgggcgt
     7681 ttgtcgagaa cgctgtcatc gagatcgacc gccttcagca tttcgctggc gcgcgccagt
     7741 tgttcggatt ttttcagtga tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg
     7801 gttttgcgcg gattcacggc gctgatggag tcctgaaata ccatctgaat atcgcggcgg
     7861 aacgctttac gctgggcgcg attgagtttc gccagcggtt cgccgcgcca gctaatattc
     7921 ccttgtgagg gagactctaa gcctaccagc agccgcgcga gggtgctttt tccgcagccg
     7981 ctgcgtccca gcagggcgac ggtttcgccg cttttcaggg tcagggaaac gttattcagc
     8041 accgcctgat gttgatgttt tccgctaaat ccaccgtggg catagtgatg ggaaaggccg
     8101 cagacgttaa gtaaggtcat gatgccagct ccataccgta gagggcgaga tgagcggaaa
     8161 ccaggctgcg cgtcaccgta tgtttggggg cgttaaacag cgtttctaca tcgccctgtt
     8221 cgacaatttt accttgtgac atcaccgcca cgtcatccgc cagacgcgcc acaacgccca
     8281 tatcatgggt gaccagcagc attcccggcg cttgtttttg cataatgctt tccagcagat
     8341 cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt cggttcatcg gcgatgataa
     8401 acggtgattc acacagcacc gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca
     8461 tctcgaacgg gtacagcttc agcacgcgcg cggcgttttc cagccccacc gcttctatgg
     8521 cagcggtaag cgtagcgtca tcggcgggtt tccctaacgc caggcaggtt tcccgcgcgt
     8581 gggtgtgcat ggtgtgcagt ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa
     8641 ttttgatgcc gcgcagggcg caaggcgaaa ccggtttgcc atcggctaaa atttcccccg
     8701 ccgtctggcg aacgccagcg ggcaaaatgc ccagcgtcgc ggcgcaggtt aatgatttcc
     8761 cgctgccgct accgccgact aacgccagca cgcgcccgcg ttgcagggtt aacgatacac
     8821 cgtgtaccag cggctgcgcg gcctgtagcg cgatattacg tagttcaatc tgttgcggca
     8881 ttagtgtgcg tgctccgtca ccagatgagg atccagatga tcgcgcagtg cgtcacccac
     8941 caggttaaag gccatcacgc tgataaacag cgccagcccc ggccagaaca tttgcagcgg
     9001 ctgggtccag atatactggc gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc
     9061 ggtcacaccg aggccaagga aagacatccc cgcgacgtgc agcatcatat ggccgatatc
     9121 cagcgttgcc agcaccagca gcgaagggat caccgcgcct gccagatgat cgacaaacac
     9181 ccgcacatgg cccgcaccgg aaagccgtga cgccagcaca aactcgcgtt ggcgtagtga
     9241 aatcaccagg ctgcgcacca tgcgtgcata ccacgcccag tgcgacaggg cgatggcgat
     9301 aattacgttg gtcagcccgg tgccgagcac gccaaccata aagaacgaca gaatcgaggt
     9361 cgggaaggtc ataaacatat cggcgacgcg catggtggcc tgatcaacgc gtccgccaat
     9421 cagtccggcg ctgccgccaa tcaccagccc taatgtcagc accagcagca ggcaggccat
     9481 taccgaaccg agcgacacgc gggtcgccgc catcagccgc gagaaaatat cacgccctaa
     9541 gtgatcggtg cccagccagt gctgcgcatc cggcgaaagc aggcgcgacg gcagatcaat
     9601 agcctgaggg tcatacggca gccaccactg gctggtaagc gcaatcagcg ccagcagggc
     9661 gataatgatc agcgccaggc gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgc
     9721 gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt tgagcaaatc gacaatcaaa
     9781 ttacagacca caaaaaccac caccatcatc agcgtaaagc actggatcac cggatagtca
     9841 cggttaaaaa tcgccgacac cgcatagcgc ccgacgcccg gccaggcaaa gatgttttcg
     9901 ataatcatcg tcccgccgat cagttcgccg atgtgcatcc ccacggcggt gatcatcggc
     9961 agcgaggcat tgcgcaggat gtggcgacgt tcggtctgtt tgtcgttcag gccgcgcaga
    10021 cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca tactggcgcg cagtaaacgc
    10081 gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag
    10141 ccgccgtaac ccatcgcggg tagccattgc agatacaccg aaaacgccat caccagtaaa
    10201 aacgccagcc agaagttagg catcgacacg ccaagaaacg cgatgaaacg tacggcgaaa
    10261 tccggcagac ggtcgcggtg gcgcgccgcc cagataccga gcggtacgga agtgagcaga
    10321 attaatacca gcgccgcacc tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg
    10381 tccagtaccg ggcgttggct ggcgaatgag ataccaaagt caagatgcag cgccttccac
    10441 aaccaggtgc cgtactggac gtacagcggc tgatccagtc ccagcatggt gcgggtagag
    10501 gccagcatct ccggcgtcgg cggcaggtta gacagacgca aataatcgag cgccgggtcg
    10561 ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac catcgggatc
    10621 agcagcagaa agcggcgtaa tacgtaacgc aacattaagg tttcaccggt ttaatctgtt
    10681 cgaacggaat ttcggtggcg ataggcgcgt aggggatatt acccagctcc ggttttgata
    10741 ccaccatcat cgagatgtag ctgataggca gataaaccgc ctcgtcatgc agacgggtca
    10801 gaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg ggtcgccagc acttcgccga
    10861 tctctttatc aatcagcggc ttatcggcta atccttgctg tgcctggaag tcagcgtgcg
    10921 acggtacgcg cattgaactc aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt
    10981 ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata gatactgctc tcttcttcgc
    11041 caatcagcga gacatctgcg ccaatctggc gcatatcagc ctgaatgatt tccgccatcg
    11101 atttgcttaa cgcatcggtg ccgatgaacg acagttcaat gcgcagcggc tgaccatttt
    11161 tctcgcggat gtctttaccc gcaggcagcg tccaaccggc tttttccagc aactctttcg
    11221 ctttctgcgg atcgtactgg cgcggtttca ggccgagatt agcgtaaggc acagaagggg
    11281 caaacagggt gtcggcgacc tgctgggtgc catataacgc gttatcaatc agcgattttt
    11341 tgtttaccgc gtaattaaga gcttcacgta ctgccagctc gttggtgggg gctttggcgg
    11401 tattgagcgc cagcatcacg gtttcgatcg gctgtgacag ttgggtgtgg taagccggat
    11461 tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt
    11521 cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg gtccgggatg acgttaaagg
    11581 tgatcttttt aatcgctggc ttttcgcccc agtagttttc gttacggacg aagacatcgt
    11641 actgattcag tttcgattcc tgcaaaatcc atggtccggt gccaatcggc gctttaattc
    11701 cgttcatggt ttcatggttt ttaaactgcg agggagcgat aaaacggaaa ggacggggca
    11761 gggccagttc ttgcaggaaa ggatagtagg cgcttttcag ggtaatttgc agctctgttt
    11821 tactgagtgc tttaacatca acaatctggt ttgccagctc cagccaggcg tgacgttgac
    11881 ggttatcgag cactgcgcgg aagttttctg ccgccgcctc ggcatcgaac ggttcaccgt
    11941 tggagaattt cacgtcatca cgcagggtga aggtccaggt tttaccatct tctgaatgag
    12001 tccagctttt tgccagccac gggatcaccg aaccgtctgc ctgatatttc accaatggtt
    12061 cataaaccat gctctgggcg aacatctggt taggcgtgta aaggtgcggg tttagtggcc
    12121 cgacattcac cggccaggcg gtggtgattt catctggtgc agcggcatgg acgataaaag
    12181 acgcacaagc cagcagcgca aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa
    12241 tggattaaaa tagatggcgg aaataagtat gacgatttta agtattcgtc atactgatta
    12301 cctgttctgg atcaatagat gggcagtcgg gtgggcggat cagttaactg aatcgatcca
    12361 ttgcacactg tcggcggtga gcgtaaaggg agtaggggtg cagatcgcca ggcttaaatt
    12421 ttcaagctga caatggctga ctgacagcga ggagtgatag gtgctgtcta cgctgacgat
    12481 ttgccaggcg ctgccgccac gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca
    12541 gaacatttcc agttgctgat caggatgcac ggcgtccatc tcagcgtgtt ccccgaggct
    12601 gaatacggcg ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca
    12661 gccgacttcg ccttcatcac tcaacagcag ggcgatatcg tcaccgctat ggcttaagtt
    12721 gaaccatagc ggcgtttccg gcgcaaatgc aggtttgcct tgttcgccat agatgatctc
    12781 cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc gttcgcgtcg
    12841 tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt ggagctgcgc ttaaggtcga
    12901 aactttcccc agaactatcc gatacatatc agggccaacg tttaatggaa aatgaaagtg
    12961 cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt ttcggtctgc ctggccccag
    13021 taaaatcgcc agtttgctac cgcctttggt ggtttccatc cagattttac acacactggt
    13081 taatggcacc gaaagtagca tccctaccgg gccgagcagc catccccaaa ttaacaatga
    13141 aagaaatacc accatggtgg acatccccag gcgatggccc atcatccgtg gctctaaaat
    13201 attgccgatg accatatgga caactaaaaa caatgcgccg accagaatac attcgtaaat
    13261 accattaaac agcagcacct gaatcattgg cggtacggcg gaaattaccg cgccgatatt
    13321 gggcacgtag ttaagcaaaa acgccagtac tgcccacatc agcgcaaact gtacccccat
    13381 cagctccagc cccagccaga cgatgacacc tgtccataaa ctgagtagcg tcttcaatgc
    13441 aagatagtgc gaaacgcctt taagtgcgcg gtgtaatccc gcgatgtgaa tctgtggatt
    13501 attcagcgca aaacgcattt tgtaagggac gtggcgcact tcaaacagca taaaaactac
    13561 ggtcatcacc agcaaaagca cgctcgccat tgccccggaa agcccggtca ttagcgctgt
    13621 ggtgaaggta accacttttt ccgagtccat ccgctgcagc attcgctccg gcgacatatg
    13681 caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt ttgcgcgtca gctccttatt
    13741 aaacttcggc agcatagaga taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc
    13801 ggttagtgcg atcagcatca ccaccactac aatcgtaatg gcaacggggc gttgtactcc
    13861 ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg gcaaaaaaca gcgccagtaa
    13921 cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc
    13981 cagcttgagc agaatgtgca tgcccgtttt atcgggttga ggggtttcca ttggggcttc
    14041 cttgtgactt tttgtattaa gtgtagcggg agtcacgcca gcaatattct gattcttact
    14101 gctgattttc acgtcagcac gtggtaaaaa tgaaacactg ttgtaaaaat gtggtgatcc
    14161 tcatgcccga acccgtagcc gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg
    14221 tctccattgt catgtttaac ttcgccagct acctcaccat cgggttgccg ctcgctgtat
    14281 taccgggcta tgtccatgat gtgatgggct ttagcgcttt ctgggcagga ttggttatca
    14341 gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc cggacgttac gccgatttgc
    14401 tgggccccaa aaagattgtc gtcttcggtt tatgcggctg ctttttgagc ggtctgggat
    14461 atctgacggc aggattaacc gccagtctgc ccgtcatcag cctgttatta ctttgcctgg
    14521 gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac gggatcgacc ctgtggggcg
    14581 ttggcgtggt tggctcgctg catatcgggc gagtgatttc gtggaacggc attgtcactt
    14641 acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt ttatcactgg ggcggcttgc
    14701 aggcgttagc gttaatcatt atgggcgtgg cgctggtggc cattttgttg gcgatcccgc
    14761 gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt tcgcgcggtg cttgggcgcg
    14821 tctggctgta cggtatggca ctggcactgg cttccgccgg atttggcgtc atcgccacct
    14881 ttatcacgct gttttatgac gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt
    14941 ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa cggcattaac cgtatcggcg
    15001 gcttaaacgt agcgatgatt tgctttagcg ttgagataat cggcctgcta ctggttggcg
    15061 tggcgactat gccgtggatg gcgaaaatcg gcgtcttact ggcgggggca gggttttcgc
    15121 tggtgttccc ggcattgggt gtagtggcgg taaaagcggt tccgcagcaa aatcaggggg
    15181 cggcgctggc aacttacacc gtatttatgg atttatcgct tggcgtgact ggaccactgg
    15241 ctgggctggt gatgagctgg gcgggcgtac cggtgattta tctggcggcg gcgggactgg
    15301 tcgcaatcgc gttattactg acgtggcgat taaaaaaacg gcctccggaa cacgtccctg
    15361 aggccgcctc atcatcttaa aacttactga ataaccagcg tattaatgat gttttctgcg
    15421 gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa tttgcatggt cagcagttga
    15481 ttacccacgt tacccagaat aacggaagag tacgccgtct ggcctttcgc ggagataata
    15541 ctgtctaact gctgcatttt gtgacctttc agctcaatgg ctttattggt taccacttgc
    15601 agctgcggat cgcggctacg ttgctgatct tccagacgct tcgccagcac cgccagatct
    15661 tctttcggat catcgcccat gatgacgatg actgctttct gcccggtggc gtcggaccag
    15721 acatgcatgt tattggcctg cgttcccagc ttaccgctct ggtcggtcat atccgctggc
    15781 agcgagaaac ttaacttgcc atcaagcagg ttgacgggat tcccggtagc gttactttcc
    15841 gcgaccgaac cctgcgccgt agcgttagtg tctttatcat cacaggccgc aagccccata
    15901 accagcaggc caattccgac atatttaacc agattgcgca ttgacttctt cctttcgata
    15961 aacggccata acggctcatt catccatctt atcacaactc tgataacgaa cctttaactc
    16021 gcctgcaaag cgttgatttc ggatttatct gccagtcttt tcaacagcat attgagtaat
    16081 acgccataca ttggcaggaa gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc
    16141 agcgcgattt ccatccagtg ttcagccata aaggcatccg ggctacgcca gaaggcaatg
    16201 aagaaaaagg ccagcgtgtc actgacgtta ccgaacagtg tggacgctgt cggtgccagc
    16261 caccagcggc gactttgacg caggcggtta aaaacgtgca cgtcgaggat ttgccccagc
    16321 gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa acaggttgaa gtgggcgagt
    16381 gcgccgaatc cctgccatga acccatatag aacagcgacg agatgacata ggagattaat
    16441 aacgcaggga tcattaccgc gaagataatg cgtcgggcca gcggtgcgcc aaaaatacgc
    16501 acggtcaggt cggtcgcgag aaagataaac ggaaaactaa acgcgcccca ggtggtatga
    16561 aaaccaaaaa tggagaccgg aagctggacc agatagttac tggaggtgat caccagcaga
    16621 tgaaataacg ataaccagaa caacgcctta tagcgttgag tttgcgagaa aacgttcata
    16681 ttgtaccttt ttgattaacc attggggtga gggaacccaa tacgtacgac acgtctgtta
    16741 tcttaacata ataacgtgct tacccttttt tcgagccgcc gcatgatact gctttacgtt
    16801 gacaatgcaa tggttaattt tcacgcaatc gttaacctgg tttgcttacg gactcacagg
    16861 gcgtaaacta gcgccgtttt tttaagtgat gagaagaaaa tgaccgatct cttttccagc
    16921 cctgaccaca cactcgacgc gcttggcctg cgctgcccgg aaccggtgat gatggtgcgc
    16981 aaaaccgtgc gcaatatgca gcctggcgaa actttactga ttatcgccga cgatccggcg
    17041 acgacccgcg atattcctgg gttttgtacc tttatggaac acgaactggt tgcgaaagag
    17101 acggatggac tgccttatcg ttatttgatt cgtaaaggcg gttgatgggg aaagattggc
    17161 ttcgatgccg ccttttcccc tcaccctaac cctctcccca gaggagcgag gggaccgatc
    17221 gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca acaatcttaa cgcattcgct
    17281 gttaccagca cggtcgcacc ggtatctgcc agcaccgcca gccatagtcc ggtcatccct
    17341 agcagcgtgg tgacgaggaa gatccctttc agccccagcg caatagtgat gttctggcgg
    17401 atattggcgt gagtggcgcg tgccagttca atcatttgca ccaggccgcg caggtggtta
    17461 tgggttaatg ctgcgtcggc ggtttccagc gccacgtctg tgccactgcc cattgcaatc
    17521 ccaatggcgg cagctttcat cgctggcgcg tcgttaatac catcaccgac catcgccagg
    17581 ggcgcatgtt gattcagctt ggtcaccgct ttgactttat cttccggcaa taggcccgct
    17641 ttaaactcca gccccagctc cccggcaatt gccgccgctg cgcgtggatt atcgccggtg
    17701 aggatcactc ctttaacccc cagcgcgttc agttcactga tggcagttgc tgcgtcggcg
    17761 cgcagggtat cctgcaatgc aatgataccc agcacgtcat cattacgtac taccagcaca
    17821 accgtttgcc cggcgctttc cagttcatta atcaaaccag caaatgcatc agcgggatgt
    17881 ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct gcgcttcaat gccagacccg
    17941 accagcgccc gctgtgattc ggcggtggga atggcgagtt cagcaacctg tgcttcgcgt
    18001 acgatggcct gcgccagtgg atgcgtcgcg ccttgctcga ccgccgccgc cagtgtcagc
    18061 agttcagatt cactaatacc cgttgccgga tgaatcgcgg taacgcgcgg tttaccgacg
    18121 gtcagcgtac cggttttatc aaacgccacc tgagtaacac gacccagctg ttccagcgcc
    18181 gctccgcctt taatcaacgc cccacgacgc gctgccgccg ccagcccgga ggtaatcgcc
    18241 gcaggcgtgg agataactaa cgcacacggg cagccaatca gcagcagcgt cagcccttta
    18301 taaatccact cctgccagct ggcggcaaac agcagcggtg gcaccagcgt caccagcagg
    18361 gcgacggcca taatcgctgg cgtataaata cggctgaaac ggtcgataaa ccgctcaatg
    18421 ggggcgcggc gctcttcggc ttcttcgatc agtttcagaa tccggtcaat ggcgctggca
    18481 cccggttctg acagcacttc cagcgttacc agacggtcta cgctggtggc tcctgcagga
    18541 actttatcgc ccgtcgcgcg ctccaccgga atggattctc cggtcagggc gctttcatca
    18601 aaactggcaa acggtgagag cagtttaccg tcggcaggca aacgcccacc tgcggcgact
    18661 tcaatcacat cgccaggccg caggctgtta atcgccacct cttcccgctc accgttacgc
    18721 aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc taacgccctg acgcgcgcgg
    18781 ctggcggccc agccttccag tcgttcacca atcaaaaaca gcagcaacac catcgcagct
    18841 tcagccgttg cgccaataaa cagtgcacca atagcggcta cgctcattaa ggtttcaatg
    18901 gcgaagtagc tgccggattt gatcagccgt aatgcctgac gagcaatcgg gtacagccca
    18961 accagcgtgg tcgcgataaa cgccagttgc ccaaacggat gattaaattg ctccagaccc
    19021 cagctgattg ccatcatcac gattagcgta atcagcggca gattctcttt caggcgcgat
    19081 gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat agcccgcttt ttgcaccgca
    19141 gattcaacct gggcacggat gtcattgtcg gcatcgacca ccagtttttc ggtggcgaac
    19201 agtacctgca cctgattcac acctgcaagc tggcgcacgg cattttctac cttgcgcgca
    19261 caggcggcgc agtccatgcc gctgactttc cagctatagc gggtgccgga gacgttttca
    19321 gagagcgttg gcgagctgga acatgcgccg tcgcagcaac agtcgttggc gttctgtacc
    19381 gtggttagcg gtttgaacgc agcaaattga ggggctttct tgccgtgatt gtcaggagtc
    19441 gacatggcat cctccggtta agttttttct cattaaccga aggatacact ctggagtcga
    19501 ctccagagtc aagttttatc agagatacag cgagcggacg atgaggaaat gaccggcaaa
    19561 gtagcaggcc gcagcaatcg cgttatctgc ccggaagcgg cggcgatagt ggctacccag
    19621 ccagacaaag ttactgataa acagcaatga agcgccaaca aacgcagaga gtgccggagc
    19681 ggtcggacgg aagaaccaca gttcacctgc cagccacacc atcaccagcg tcatgccgat
    19741 aaacgtgcag ataggccaac gatactcttc cagccgcgtc cagataatcg ccagtaacag
    19801 cgcgcccaga accagcaaca ccagtggcag aggccagaag aaagagagcg tcatctgact
    19861 ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac gcgccgatgg cgtacatcag
    19921 acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt gaggcgcaca gccctgccag
    19981 caccagatag ctaatggcgt cgaacatcgg cgcttgccag gccagtaaca gcaggagaag
    20041 aagggttaac ggtttaaaca cccagcgttg ccaggttggc ccacgatacg atgcatccac
    20101 agatagccat gcggaaagac agacagcgat aaacgaccaa agcatcttag ttccttgaat
    20161 tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt ccagtttagt ggcgagggtg
    20221 gacggatgac aacaccataa ctggcagggt atgcttattt ccgcattttc cgatgaggga
    20281 taaagatgag caaaccacct cttttcttta ttgttatcat tggcttaatt gtcgtcgccg
    20341 catcgtttcg ttttatgcaa cagcgacggg aaaaagctga taatgatatg gctccgctcc
    20401 agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc gattaacgat cgccgttcgc
    20461 gccagcagga agtgactccg gcaggcacca gtatgcgcta tgaggcaagc ttcaaaccgc
    20521 aaagcggagg aatggagcag acgtttcgcc tcgacgccca gcagtaccac gccctgacag
    20581 tgggcgataa aggtacgctg agctataaag gaacgcgctt tgtcagcttt gtaggcgaac
    20641 aataaggtta ttttttaact ttaaatttct tctgccagac cagcagttca aacacgccga
    20701 aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg gccatctttc ggtaaagtgg
    20761 atttcaatag cgccagttgc ataccatgca tcagcacggt aaaaatcagc gccacgttaa
    20821 cgaaaatatt cagcgggcgc gggaagggat gcaccagatt gaggattaaa aatccccaaa
    20881 cgcagagcat taacaaacga ccaatattaa tcagcatcac tttctccttg tgcttcgcgt
    20941 tgatacagcc gataagccac ctgacccgcc actttttccc gatgtaatga ccagtttgcc
    21001 ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat aaatcagggc ttcgtcagcc
    21061 agccagccgt tatcttccag taaatttatc gtctcttcta acaagccacg gcggaacggt
    21121 ggatcgacaa acacgatatt atgcggtgtg cctttttgcg ccaggaatga cattgcgttg
    21181 ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca gattcttaat taactgctga
    21241 gaaactgcgc gatccatctc aatcaacgtg gcccccgcag cgtagcgcga taacgcttcc
    21301 agccccagcg cgccgctccc ggcgaagcaa tccagacatt gggcgtcaac aatgaccgga
    21361 gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg tggggcgcag acctgggcta
    21421 tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa taatgcggat ttggccgctg
    21481 ccggaatgat tcggtttttt catgataaat tgctcaatcc gccagataac acatacttgc
    21541 aggcggtgat gtgaattaag ttaagtgata gactatttca tcattttttt agctgctatg
    21601 tacatagcgt taacgctgtg ccatgaagca acagcgagga gtgtagtcgc aaatggcgaa
    21661 agaaaaaaaa cgtggctttt tttcctggct gggctttggt caaaaagagc agaccccgga
    21721 aaaagagaca gaagttcaga atgaacaacc ggttgtagaa gaaatcgttc aggcgcaaga
    21781 gcctgcgaag gcttctgaac aagccgtcga agagcaaccg caggcgcata ctgaagccga
    21841 ggcggaaact tttgctgccg acgttgtgga agtcactgaa caggttgctg aaagtgaaaa
    21901 agcgcagcct gaagcggaag tcgttgcaca gccggaaccg gtcgtagaag aaacggcgga
    21961 gccagtggct atcgaacgtg aagagctgcc gttgccggaa gacgtcaacg ccgaagaggt
    22021 ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag attgtcgaag cggcggaaga
    22081 agaagcggct aaagaagaaa tcaccgacga agagctggaa gcacaggcgc tggctgccga
    22141 agcggcagaa gaggcggtga tggtggttcc tccggtagaa gagcagccgg tggaagaaat
    22201 cgctcaggag caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct
    22261 gttaaaaacc aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat
    22321 cgacgatgat ctgtttgagg agctggaaga acagctgttg atcgccgatg tgggcgtgga
    22381 aaccacacgt aaaattatca ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga
    22441 tgccgaggcg ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga
    22501 tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg
    22561 tgtgggtaaa accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc
    22621 ggtgatgctg gcggcgggcg atactttccg tgcagctgcg gttgaacagc ttcaggtctg
    22681 gggtcagcgt aacaatatcc cggtgattgc ccagcatact ggtgcggatt ccgcttcggt
    22741 tatcttcgac gccattcagg ctgcgaaggc gcgtaatgtt gacgtactga ttgccgatac
    22801 cgccggacgc ctgcagaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt
    22861 gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg ctgactatcg atgccagcac
    22921 cgggcagaac gcggtaagcc aggccaaact gttccatgaa gctgttggct taaccggcat
    22981 cacgctaacg aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca
    23041 gtttggtatc cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt
    23101 taaggcggac gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt
    23161 gaacatgtca gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat
    23221 atgcagccgg gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc
    23281 ctgaagctga tctgtgggat tgagcggccc agcgccggga aaatctggtt tagcggccat
    23341 gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt
    23401 ttccaggatc accatctact gatggaccgt actgtctacg ataacgtggc aatcccgctg
    23461 attatcgccg gtgccagcgg tgacgatatt cgtcgccggg tgtcggcggc actggataaa
    23521 gtcgggctac tggacaaagc gaagaacttc cctattcagc tttctggcgg tgaacaacag
    23581 cgtgttggca ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg
    23641 actggtaacc tggacgacgc gttgtcggaa gggattctac gtctgtttga agagtttaac
    23701 cgcgttgggg taaccgtatt gatggcaacg cacgacatca acctgatctc gcggcgttcc
    23761 tatcgcatgc tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg
    23821 cgatgcaatc aatcatattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt
    23881 cggcggctct ggcgacggcg gtcgtaacgc gccaaaacgc gcgaaatcct cgccaaaacc
    23941 ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc tatgccttcc acggcgcatt
    24001 gcaggatctg aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc
    24061 cctgacgctg cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca
    24121 atattatccg tcaccgcaaa tcactgttta cttgcaaaaa acgctggacg atgacgccgc
    24181 agcaggcgtg gtagcacagc tacaggccga gcaaggcgtg gagaaagtga actatctttc
    24241 tcgtgaagac gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat
    24301 gctggaagaa aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg attttcaggg
    24361 gacggaatca ctgaatacgc tgcgtgatcg tatcacccag attaacggca ttgacgaagt
    24421 gcggatggat gacagctggt ttgcccgtct ggcggcgttg accgggctgg tcgggcgcgt
    24481 ttccgcgatg atcggcgtgt tgatggtggc agcagtgttc ctcgtcatcg gcaacagtgt
    24541 gcgtctgagc atctttgctc gccgtgactc cattaacgtg cagaaactga ttggtgcgac
    24601 ggatggtttc atcctgcgcc cgttcctgta cggaggcgca ttgctgggat tttctggcgc
    24661 attgctgtca ttaattttgt cagaaattct ggtactgcgt ttgtcatcgg cggttgcaga
    24721 agtggcacag gttttcggaa cgaagtttga tatcaatggt ttatcgttcg atgaatgcct
    24781 gctattgctg ctggtatgct cgatgattgg ctgggtggca gcgtggcttg ccacggtaca
    24841 acatttacgc cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg
    24901 gttccgtagc agggaaagag atcccgttgt ctcttcccgg tatttcatct ctatgtcaca
    24961 ttttgtgcgt aatttattca caagcttgca ttgaacttgt ggataaaatc acggtctgat
    25021 aaaacagtga atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga
    25081 agcgccagaa gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt
    25141 tagccccagt tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt
    25201 cggctgacga ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag
    25261 ctaaaacgct gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg
    25321 gctatggcct gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag
    25381 tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc cttcgccgtt cactggatca
    25441 aagcagagat ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgaccacca
    25501 aagcgcagcg caaactgttc ttcaacctgc gtaaaactaa gcagcgtctg ggctggttta
    25561 accaggatga agtcgaaatg gtggcccgtg aactgggcgt aaccagcaaa gacgttcgtg
    25621 agatggaatc acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt
    25681 ccgacagcca gccgatggca ccggtgctct atctgcagga taaatcatct aactttgccg
    25741 acggcatcga agatgataac tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc
    25801 aaggtctgga cgaacgcagc caggacatca tccgcgcgcg ctggctggac gaagacaaca
    25861 agtccacgtt gcaggaactg gctgaccgtt acggtgtttc cgctgaacgt gtgcgccaac
    25921 tggaaaagaa cgcgatgaaa aaacttcgcg ccgctattga agcgtaattt ccgctattaa
    25981 gcagagaacc ctggatgaga gtccggggtt tttgtttttt gggcctctac aataatcaat
    26041 tccccctccg gcaaaacgtc aatccccacg cagattgtta ataaactgtc aaaatagcta
    26101 ttccaatatc ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagt
    26161 catgcataaa acgaaataaa gtgctgaatc acaacaccac aacacacgta ataaccagaa
    26221 gaatggggat tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc
    26281 tggcattcag caatatggct ctggcagaag atattaaagt cgcggtcgtg ggcgcaatgt
    26341 ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg cgcagagcag gcggttgcgg
    26401 atatcaacgc taaaggcggc attaaaggca acaaactgca aatcgtaaaa tatgacgatg
    26461 cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat
    26521 atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc gtctgacatc tacgaagacg
    26581 aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga gctgaccgcc cgtggctatc
    26641 agctgatcct gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata
    26701 ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca cgacaaacag caatacggcg
    26761 aaggtctggc gcgagcggtg caggacggcc tgaagaaagg caatgctaac gtggtgttct
    26821 ttgatggtat caccgccggg gaaaaagatt tctcaacgct ggtggcccgt ctgaaaaaag
    26881 agaatatcga cttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc
    26941 aggcacgcgc ggcagggctg aaaactcagt ttatggggcc ggaaggtgtg gctaacgttt
    27001 cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact
    27061 acgatcaggt tccggcgaac aaacccattg ttgacgccat caaagcgaaa aaacaggacc
    27121 caagcggcgc attcgtttgg accacctacg ccgcgctgca atctttgcag gcgggcctga
    27181 atcagtctga cgatccggct gagatcgcca aatacctgaa agcgaactcc gtggataccg
    27241 tgatgggtcc gctgacgtgg gatgagaaag gtgatctgaa aggctttgag ttcggcgtat
    27301 ttgactggca cgccaacggc acggcgaccg atgcgaagta atcattaatc ggcaactttg
    27361 ggttgccgcc aaattgctaa tatcgagtac gttgcttcat gccggatgcg gcgtaaacgc
    27421 cttatccggc ctacaagatc caaagaaatc agtaaattgc aacacacatt gtaggcctga
    27481 taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca acttgacgat
    27541 taacgcttct cccagccgcc ctgttgcgcc gtaaacccca gcgcctgcat aaacgccgtc
    27601 atcacaccgc gatcttccac gcctgcatcc gccatccacc agcatgaaac ggcaggattg
    27661 ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacggcgggt gacttcccgc
    27721 acgcgcaggg aatccagtgc tccctcggtg ccgcttaagg ttacccgcac ggccgctagc
    27781 aggcgctcgt taaaacgcgc ggcgtagatg cggtggttat cgtcaacctg taaagaggaa
    27841 ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct aaagttttct
    27901 aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt
    27961 agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg tgattaattt
    28021 tttaacagca ataattacaa agtt
//
LOCUS       DAPPNJ010000043        28038 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000043 DAPPNJ010000000
VERSION     DAPPNJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.043"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..612
                     /locus_tag="RPM73_001492"
     CDS             <1..612
                     /locus_tag="RPM73_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDX6519865.1"
                     /translation="AGLAWVAKYLEITATKYDSPQMYVAIGLLLLCMYGLSKDINKIN
                     AVIAGVIYLFLLSLVAIVVASLAPVPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRF
                     IIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEE
                     LWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(648..1604)
                     /locus_tag="RPM73_001493"
     CDS             complement(648..1604)
                     /locus_tag="RPM73_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDX6519866.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1604..2845)
                     /gene="clsB"
                     /locus_tag="RPM73_001494"
     CDS             complement(1604..2845)
                     /gene="clsB"
                     /locus_tag="RPM73_001494"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDX6519867.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2842..3603)
                     /locus_tag="RPM73_001495"
     CDS             complement(2842..3603)
                     /locus_tag="RPM73_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDX6519868.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQVPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3736..4146
                     /locus_tag="RPM73_001496"
     CDS             3736..4146
                     /locus_tag="RPM73_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDX6519869.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4108..5214)
                     /locus_tag="RPM73_001497"
     CDS             complement(4108..5214)
                     /locus_tag="RPM73_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6519870.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5225..6358)
                     /locus_tag="RPM73_001498"
     CDS             complement(5225..6358)
                     /locus_tag="RPM73_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6519871.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6351..8087)
                     /locus_tag="RPM73_001499"
     CDS             complement(6351..8087)
                     /locus_tag="RPM73_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6519872.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8080..9075)
                     /gene="hlyD"
                     /locus_tag="RPM73_001500"
     CDS             complement(8080..9075)
                     /gene="hlyD"
                     /locus_tag="RPM73_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDX6519873.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9078..9749)
                     /gene="cecR"
                     /locus_tag="RPM73_001501"
     CDS             complement(9078..9749)
                     /gene="cecR"
                     /locus_tag="RPM73_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDX6519874.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9978..11342
                     /gene="rhlE"
                     /locus_tag="RPM73_001502"
     CDS             9978..11342
                     /gene="rhlE"
                     /locus_tag="RPM73_001502"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDX6519875.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(11574..12056)
                     /gene="ybiA"
                     /locus_tag="RPM73_001503"
     CDS             complement(11574..12056)
                     /gene="ybiA"
                     /locus_tag="RPM73_001503"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDX6519876.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12176..14326
                     /gene="dinG"
                     /locus_tag="RPM73_001504"
     CDS             12176..14326
                     /gene="dinG"
                     /locus_tag="RPM73_001504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDX6519877.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14354..15316
                     /gene="ybiB"
                     /locus_tag="RPM73_001505"
     CDS             14354..15316
                     /gene="ybiB"
                     /locus_tag="RPM73_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDX6519878.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15457..16542
                     /gene="hcxB"
                     /locus_tag="RPM73_001506"
     CDS             15457..16542
                     /gene="hcxB"
                     /locus_tag="RPM73_001506"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDX6519879.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16771..17031)
                     /locus_tag="RPM73_001507"
     CDS             complement(16771..17031)
                     /locus_tag="RPM73_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6519880.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17296..17562)
                     /gene="ybiI"
                     /locus_tag="RPM73_001508"
     CDS             complement(17296..17562)
                     /gene="ybiI"
                     /locus_tag="RPM73_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDX6519881.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17636..18313)
                     /gene="ybiX"
                     /locus_tag="RPM73_001509"
     CDS             complement(17636..18313)
                     /gene="ybiX"
                     /locus_tag="RPM73_001509"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDX6519882.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18355..20637)
                     /locus_tag="RPM73_001510"
     CDS             complement(18355..20637)
                     /locus_tag="RPM73_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDX6519883.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20902..21162)
                     /locus_tag="RPM73_001511"
     CDS             complement(20902..21162)
                     /locus_tag="RPM73_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6519884.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21438..22364
                     /gene="rlmF"
                     /locus_tag="RPM73_001512"
     CDS             21438..22364
                     /gene="rlmF"
                     /locus_tag="RPM73_001512"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDX6519885.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22361..24586)
                     /gene="ybiO"
                     /locus_tag="RPM73_001513"
     CDS             complement(22361..24586)
                     /gene="ybiO"
                     /locus_tag="RPM73_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDX6519886.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     F"
     gene            complement(24703..25425)
                     /gene="glnQ"
                     /locus_tag="RPM73_001514"
     CDS             complement(24703..25425)
                     /gene="glnQ"
                     /locus_tag="RPM73_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDX6519887.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25422..26081)
                     /gene="glnP"
                     /locus_tag="RPM73_001515"
     CDS             complement(25422..26081)
                     /gene="glnP"
                     /locus_tag="RPM73_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDX6519888.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26220..26966)
                     /gene="glnH"
                     /locus_tag="RPM73_001516"
     CDS             complement(26220..26966)
                     /gene="glnH"
                     /locus_tag="RPM73_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDX6519889.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27370..27873)
                     /gene="dps"
                     /locus_tag="RPM73_001517"
                     /gene_synonym="pexB"
     CDS             complement(27370..27873)
                     /gene="dps"
                     /locus_tag="RPM73_001517"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDX6519890.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6631 a         7296 c         7473 g         6638 t
ORIGIN      
        1 gcaggactgg cctgggtagc gaagtatctc gaaattacgg caacaaaata tgattcaccg
       61 caaatgtatg tcgccatagg attattatta ctttgtatgt atggcttaag taaggatatc
      121 aacaagataa atgccgtcat cgcaggcgta atatatctat ttttactctc tttggtggcg
      181 attgtcgttg ctagtttagc tcctgtacct gccattatcg tcgtgttcag cactgcgggc
      241 gcgatgtttt taatcagtat gctggccggt ttattattta atgttgatcc tggttctcac
      301 cgttttatca ttatgatgac gttgacaggg ttggccctgg tgatcatcat gaatgccgca
      361 ttaatgagtg aacgacccgt ttgggtaata agttgcttaa tgattgtgtt atggtcaggc
      421 attatctcgc atggacgaaa taagctcctt gaattggcgg ggaaatgcca tagtgaagag
      481 ttgtggagtc cggttcgttg cgctgttaca ggtgcattaa cactctatta ctattttatc
      541 ggcttctttg ggatacttgc cgcgatagct ataacgcttg tctggcaaag gcatacgcgt
      601 ttttttcatt agtcgtaaat gcagatacta aagaacttaa ctaaacttca catcgccgct
      661 tcatttttcg cccgtagctt cttcgcctgg ctttccagta gcaggtaaca gatcagcgcc
      721 agcagcagcg ggataaagta atacaggacg cgataagcga gcagggcggc gataatgctc
      781 cctttcgagg tatgctcccc agccagtagt gcgataaaca ccgcttccag cacaccgatc
      841 cccgccggaa tatgcacgat gacgccagca atactactaa ccagtaacac gcccagtaca
      901 aagaaatagt tcacgctttg accaagtaac agccagataa tcgcccccat taccatccag
      961 ttaacgctgg aaatcagcat ctgagcgagg gcgaatttcc atgaaggcag caccagcttt
     1021 tgccctttga tggtcatatg gcggtgcttc gcgaaagcgc aaaaccacaa ataaaccgcg
     1081 ataatcatca gtaagccaat gccgagaata cgcagcgtag tttgatcgac ataccagtga
     1141 tccggcaact ccaccacgcc tgcggtaaag ataatccctg ccagtaaaat atagcccagc
     1201 cagttggtgg taatactgag cgagaaaatc cgcgtaatag tgctgcccgg taaccctagt
     1261 cgggagtaca aacgatagcg cataccaatg ccgccgaccc aggtactgag cgtcaggttg
     1321 aaagcgtagc agataaacga caccagcatc acctggcgct tcgccagttt gtgaccgcag
     1381 taaaagcggg cgagcaggtc atagcagccg taaatcagat agctgacgac caccagcccg
     1441 accgcactaa gcagcgcaac gcgattgtag tcgcggatga ccttccagac ctcttcccag
     1501 tccacttttt tggcgtagac caccagcaac acaatcaccg cgataaaaaa cagccaggtg
     1561 aggatcttct ttgctaagcg ccagcgcggg tgtgatttac tcatcagggt tttacccccg
     1621 tgttttcagt ttctacccga tcctgcgttt ccattgtcgg ttgtgcgggc ggatcaacct
     1681 gcgccagacg tggtgtgtgt gccggaagcc agccaaccag cgccgggaag tggcgtaaaa
     1741 agtggaacgc cagcacgctt ttggtcaggt tccaccaggt gcgtttcggc agcatggtct
     1801 catcgacctg ctgacaatct gcggcaataa tgccgttcag attatcgcgc agcgtctggt
     1861 taaaatgacg atcgtggatg atgacatttg cttcgagatt cagtgacaaa ctgagcggat
     1921 cgagattact ggaccctact gtcgcccagt gatcgtccat caatgccact ttgccgtgga
     1981 gcgggcggcg gcggtactca aatacctgaa cgccgccttt aaccagatag ttatacagca
     2041 agcgcgcacc gactctgaca atcggcatat ccggttcgcc ctgaatgatc agtttgatcc
     2101 gcaccccgcg ccgtgccgct ttacgcaagg cgtgtaaaaa tcgatagccg gggaagaagt
     2161 aggcgttggc gataatgact tctcgccgcg cctgagtgag cattttcaga tagtgacgtt
     2221 caatatcatc gcgatgttct tcgttatcgc gccagaccag caatacctgc gcttctcccg
     2281 gctggcggtt ctcttccgct ttgtgatgac gtcgccacca gcgtcgtgcc gcgctctgtc
     2341 caggcaggtt ttccagctca aactggagaa tatcttcgac aatcggccct tcaaggcgta
     2401 ccgcgtaatc ctgtttagcc tctggaccgt agctggacat atgctcggcg gagtaattca
     2461 gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat tttgcgatgc atccggcgaa
     2521 acacattggt gcgcatacca aaaaggcgag ggcggggatc gtagtagcga aataccacgc
     2581 cagctgccgt cagttcattg acaaactcat cgctgagatc cggcgaaccg tagccatcca
     2641 gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag tagtgccgca tgcagctgtt
     2701 tgccgacgtc atcctcaaac cagataaacg tttcaagaat gatgcgttct tgtgcctcgc
     2761 caatcgcctt aaacaccgcg ggataatatt gctcgccgtt ttccagcaac tggatcttat
     2821 tgccttcgcg ccagctacat ttcataaatg aatctccgca cttaaagggg catgatcaga
     2881 aaggtgtcgc catgtccgca gcggcaacgc ggttggcgcg ctggcgctgg catttttgac
     2941 gtagatcctg tccagtcgta gtagaggaac ttgcaccgga aacgtgcgcg ccgggcgtcc
     3001 gtggacgcgg gtaaaaatct catccagtcc ggcctgcact tttaacggat gattagcttt
     3061 ttgccgccag tcattgaaat cacccgccac caataccggt tcgccgtccg gtagctcatt
     3121 cacccattcg gcgagcatcg caagctgcgc ctgacggtgc gcctcacgca ggcccagatg
     3181 tacgcacatc acatgaatcg cttttccggt catcggtggc acaatgcggc agtagagcac
     3241 gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc tcataatgtt caatgggata
     3301 acgcgacagt acggcgttgc cgtgatgccc ttccgggtat acggcattgc gaccgtaggc
     3361 aaaatcgctc cacatcgtgt cggcgagaaa ctcgtagtgc gaggtatcgg gccagttttc
     3421 cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc tgcaggcaaa caatatcggc
     3481 gctgacggta cgcaccgcgt cgcgaagttc cggcaaaatg aagcgtcggt taaacgcggt
     3541 aaaacctttg tgaatgttga tggtgagcac cttgaacgaa aattgttgtg tttgatcggg
     3601 cataattttc ctgtctttgc ctcttatctc attgaaatag tgtagtcggc gtcacaaaaa
     3661 ggtgcggtct tacggaattt tccgtaaagt tcggtactct gagtaagtag agataaattc
     3721 ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg tcgcaactgg tctaagttgc
     3781 tggcagatta tgttgcattt actggtagtg gcgctgctgg tggtgggctg gatgagtaag
     3841 actctggttc acgtcggcgt gggattatgc gcactgtatt gtgtcacggt agtgatgatg
     3901 ttggtgtttc agcgccaccc cgagcaacgc tggcgtgagg tggcagacgt gctggaagag
     3961 ctgaccacga cctggtattt tggcgcagcg ctgattgtgc tgtggctgtt gtcccgtgta
     4021 ctggaaaaca actttttgct ggcaattgca gggctggcaa tccttgccgg cccggcggta
     4081 gtgtctttgc tggcgaaaga taagaagtta catcacctta cgtctaaaca tcgcgtacgc
     4141 cgctgaccct gtcgtggccg ttatcaccag tagcggccac aaactattcc acacaatatc
     4201 cagactcgca tccttcaaat aaatctgctt ggtaatgtcc gtaaagtggc gaatagggtt
     4261 aatccacgtc aggttttgca gccatactgg catgttttcc accggagaaa cgtaaccgga
     4321 aaggagaatg gcgggcatca taaaaacaaa cacgccgata aacgcctgct gttgtgtcga
     4381 gcagagtgat gaaatcaaca gaccgaatcc caccagcgat aaaccgtaaa tcaccatcgt
     4441 aaagtagaac agcgccagcg atccggcgaa ggggatttga tacgcccaga taccaatcgc
     4501 cagcacaatg gtggcctgga aggtggcgac aattaacgcc ggtacggctt tgccgatgaa
     4561 gatctgccag gtggtgagcg gcgaaaccag tagctgatcg agcgtacctt gttcacgttc
     4621 gcgggcgacg gaaagtgaag tgacgatcat tacgccgata gtggtgatca tggcgatcag
     4681 cgacggcacc acaaaccatt tgtagtcaag attcgggtta taccagttgc gtaccaccag
     4741 ctcgctgttg ttaggtttcg gttttccttc cagcagctcc tgctgataat ttttgacgat
     4801 ctgttgcagg tagttggcgg caatttgcgc actgttggag ttacgcccgt cgaggatcaa
     4861 ctgcaacggc gcggtctgga aggtatccag tttgcgcgag aagtcagccg ggaaacgcac
     4921 cagtagtaac gccttttgtg tgtcgatggt tgggcggatc tcctgtgggc ttttcagcag
     4981 cagcacatga gtaaaggcgc tggcgcgggc aaaacgttgg gtcagctcca ccgaatgctc
     5041 gccgttatct tcatcgtaga tggcgatggt ggcgttagtc acttccagcg tggcggcgaa
     5101 cgggaacaga atcacctgaa taagcacagg taaaatcaga atcgcgcggg tttgcggttc
     5161 gcgcagcagc gactgcaact ctttgcggat taacgtccat aagcgatgaa acatgctctt
     5221 ctccctaatc cagccgacgt ttggttttca gccacgtcag gccgataaac atcaccgccg
     5281 aagcgatcaa aaacagcacg tttaccacca gcaccactgg aatattcccg gcgaggaaca
     5341 ggctttgcag ggtgctgacg aaataacgag cgggaataat gtacgtcacc gcgcggatca
     5401 ccgcgggcat actgtcgatc tgaaaaataa agccggaaag cataatcgac ggcagaaaag
     5461 cggcgttcag ggcgacctga gcggcattga actggttgcg ggtaatcgtg gaaatcagca
     5521 gccccatccc cagggtactg agtaaaaaca ggctggagat aaaaaacaga atcagcagcg
     5581 acccgcgata cggcacgccg agaataaaca ctgacaccag catacacagc aacatcgcca
     5641 gcatcccgag aaagtaataa gggatcagct tacacagcag cagttccgtg cgggtaatct
     5701 ccgtagagag cagagcctcc atggtgccgc gttcccattc tcgcgccacc accagcgagg
     5761 tgagaatcgc gccgatgacc gtcatgataa tggtcaccgc accggggata atgaagtgct
     5821 ggctaatcgc cgccgggtta aaccagtagc gggtttgtac atcaataagc ggttcaaaag
     5881 tctgcccgtt gtcctccgct cgctgcattt gccagatctg ccagatccct tcgacatacc
     5941 cctgtacaaa gttagcggta ttcggctcac tgccgtcggt gatcacctga atcggtgcgg
     6001 tggcgttggc gcgctccatc tgttccgcaa aatccaccgg aataaccacc agaccgcgaa
     6061 ttttccccgc ctgcattttg gcgatcagtt cctgacggtt atcgctgatg gtggcgtcga
     6121 tgtagggcga accggtcatg gtgtgggtga aatccagcgc cgcttcgcta cgctgttcca
     6181 gtaaaatccc gacccgcagc ttgctggagt cgaggttaat gccgtaacca aaaataaaca
     6241 gcagcaacag cgggatcact accgcaatca gccagctact cggatcgcga acgatctgcc
     6301 gcgtctcttt gacgcacagc gcccgcacgc gacgccagga aaggatcggg ttactcattg
     6361 ctatgctcct tatcccagtc gtggatcaac tgaataaagg cttgctccat ggtgggatcg
     6421 ggttgctcat cgttagccga ctgtgctttc aaatcgtccg gcgtgccgct ggcgattaat
     6481 ttcccgcggt acaccaggcc gatgcggtcg caatattccg cttcatccat aaagtgggtg
     6541 gtgaccatca ccgtgacgcc tttctctacc atgctgttga tgtgcagcca aaattcacgg
     6601 cgggtgaggg ggtcaacgcc ggaagtcggt tcgtcgagaa acagaatatc cggttcatgc
     6661 atcagcgaac aggccagcgc cagccgctgt ttaaaaccta atggcagttc atcggtggcg
     6721 tgtgaggcaa tacttttcag gccgaacgcc tcgctcatac gggagatttt ttcgttctgc
     6781 gcccgaccgc gtaagccata cacaccagag aaaaagcgta aattctgttc gaccgtcagg
     6841 ttgccataga gcgaaaactt ttgcgccata tagccgagat gctggcgcgc tttaccggaa
     6901 ctctctttca gatccatccc cagcaccagc gcctgaccgg aggtcggcac cagcaagccg
     6961 cacatcatct taaaggtggt cgatttcccc gcgccgtttg gcccgagcag accaaaaatc
     7021 tcccctcgtt taacggcaaa gttgacgtga tcggtggcgg caaaatcacc aaatttcttg
     7081 gttagttctt tcgcttcgat caccgtctcg ccgggtgtgc cttctaccgt atgcaatatt
     7141 gcacccagcg gcgattccga ggttccggca ccgcccagca aatcaataaa cgcatcttca
     7201 aaacgcggcg tagtttcgtt gatgttgatt tccggcatcc cttcggcatg gcgaatatcg
     7261 tctggtgtgg cctctttttt gaggatcaga cgtaccgatt tcccctgaat catgccgtcg
     7321 ctgacctgcg gcagtttcaa ggcgcgttgc aacagtttgc ggttgccctc gtgtggactg
     7381 gtcatcagaa agctgcgtcc ggccatggtt tgtgtcaggg cttttggttc tccctgatac
     7441 agcaactcgc cttcgttcat cagtaacacg tcacggcact gctcggcttc gtcgagatac
     7501 gaggtactcc agaggattaa catcccttcg cccgccagct catgcaccat ctgccacagt
     7561 tcgcgccgtg agatagggtc aacgccgacg ccgggttcat cgagcagcaa cactttcggt
     7621 tcgcccacca gggtacaggc cagaccgagt ttttgtttca tcccaccgga gagcttgccc
     7681 gccaggcgtc cggtaaacgg cccaagagac gtaaactcca gcaggcgagc aaaagtttgc
     7741 ttacgtgcct cgccggtgac gctgcgcaaa tccgcgtaca gattgaggtt ctccatcacc
     7801 gtgagatctt catacagacc aaatttctgc ggcatatagc cgagcacggc gtgcagcgcg
     7861 ccgtcgtttt tgattggatc aaagccaatc accgtggcat tgccgctgtc gggtttcagt
     7921 aatcccgcca gcatccgcat tagcgtggtt ttacctgcac cgtccggccc caccagcccc
     7981 gtcacataac cggcgtgaat ggtacaatcg agcggcgcga cggcgggctt gtccatgccc
     8041 ggaaagcgtt tttccaggcc gttcagcgtg ataacggcat cattcatgtc ctgcctcgtt
     8101 accgaattgc accgtcaccg gcattccctg gcgtaacgca tcatcggcgt cggtcaccac
     8161 aatacgcagg cgatagacga ggtcggtacg cagatccggt gtttcgacgg ttttcggggt
     8221 aaattcagca gtcggcgaaa cgaaaccaat ctgtccgtga tagggcttgt ccgggcgacc
     8281 atcggtataa agcagcactt tgcgccccgg ctgggcctgg tcaagattcc gttcatcaac
     8341 ataggcacgt acccacaccg gacgcgttag tgaaacggta aacaccgtgc caccttcatt
     8401 gagaaccgtg cctggctcca ccgcgcgcgt taacagcgtg ccatcagacg gtgctatcaa
     8461 cgttgagtcc tgtaaattca gctccgcctg cgccagttgc gcctgcgcct gttcgaggct
     8521 ggctttcgcc tgagcgatgt cctgttcacg gttaccggaa cggtactgac gcaatttatc
     8581 ctgtgctgat ttcagcgttg cctgtgcctg gtcgcgcgag gagcgggcat tttccaggtc
     8641 atttgccgaa atggtgcggc ttttccacag tccttgctgg cggttataga agttctgcgc
     8701 atagtcatag gcggcttgag cctgtttcac cgctgcggcg gcctgagcga tttcttcatc
     8761 gcgatacccg gcaagcatca gatcatactg cgcctgtgcc accgagacgc ccgctttcgc
     8821 ctgcatcagg gcaatctcat acggcttgtg atccagttcg cccagcacct ggcccgcttt
     8881 gatcgcatca ccttcgtcca ccgccagcga ttcaacgcgc cccccaacac ggaaactaag
     8941 atttaccgta cgaatatcca cgttgccata cagcgtcagg ccgttatcct ggcggctttg
     9001 ataccaccag tagcctccgg caaccacggc ggcaagtacc actaccgcca atccgatcac
     9061 gacaggtttt ttcatcacta caaactcctt tgcgataatc cttgcaaaat caggtcgata
     9121 tgacaggtca ccgtctggtt gatcagttcg gtcttttctt catcgaacgc ggtccagccg
     9181 gtacgtaaca gaattgtttc tttgccaaga cggaacgcca gaatctcacc aatcaacgca
     9241 tgagtatgga ggatcatgcg ggtatcgttg gcgtcgcagc cggtccaggc ggcaatcagg
     9301 cgtgtcaggt ggctgtgtag cgggctaatc acctgttcgt gcaccaggtg ataggctgcc
     9361 gtgggagaga gctgctcacg ggagataaac ttgctgaggt tgacggtgtc atcctgggtg
     9421 agcagcttaa tcatgttcct gcaggcgcga aggatcagtt cacggatggc tgcgcgatca
     9481 ggctgtggtt gtgcgaacaa gcgttcggcc tcttcggcgt gcggacggaa ctgctcgcca
     9541 ataaaatcgg caatccactg ggcgcaggcg aggtacaaat cttctttcga accgaagtag
     9601 taggtgatgg cagcgatatt ctgcccggcc tgggcggcta tctcgcgggt ggtggcgttc
     9661 attccatatt caccaaactg cgccagtgcg gcagcaatca gctgtttttt cgcctgttca
     9721 cccttgattg tcatggcagg attattcatc gcacagtcca ttcttaatca aatgattgat
     9781 taagattatg actccatagg ggagttgtcc agtatggcta agaattttag ctacgccagt
     9841 cacagggata atttatgcgc tgcgtcacaa aaactgctac actccgctcc ctcatgacat
     9901 tgtggttttt gtcattttcc ttttcagtat ctccctgaaa actacaccgg taacggtcgg
     9961 ggcggttcgg agtagttatg tctttcgatt ctttgggttt aagccctgat atcctgcgcg
    10021 ccgttgccga gcagggttac cgtgaaccca cccctattca gcagcaggcg atccctgcgg
    10081 tgctggaagg ccgcgacctg atggctagcg cccagaccgg taccggtaaa acagcgggct
    10141 ttacgctgcc gctgttgcaa cacctgatca ctcgccagcc gcacgccaaa gggcgtcgtc
    10201 cggtacgtgc gctcattctt accccgaccc gtgaactggc tgcgcagatt ggcgaaaacg
    10261 tccgtgatta cagcaaatat ttgaacattc gttcgctggt ggtgtttggc ggtgtcagca
    10321 ttaacccgca aatgatgaag ctgcgcggcg gcgttgatgt tctggtggca acaccgggac
    10381 gtttgctgga cctggaacat caaaatgcag tgaagctgga tcaggttgaa atcctcgtcc
    10441 tcgatgaagc tgaccgcatg ctcgacatgg gctttatcca cgatatccgt cgcgtgttaa
    10501 caaaactgcc agcgaaacgc cagaacctgc tgttctctgc gaccttctct gacgatatta
    10561 aagccctggc ggaaaaactg ttgcacaacc cgctggaaat cgaagtggca cgccgcaata
    10621 ccgcgtctga tcaggtgacc cagcacgttc actttgtcga taagaaacgc aaacgcgaat
    10681 tgctgtcgca catgattggt aaagggaact ggcagcaggt gctggtgttt acccgtacca
    10741 aacacggcgc taaccatctg gctgagcagc tcaataaaga tggcatccgt agtgcggcga
    10801 tccacgggaa taaatcgcaa ggtgcgcgta cccgtgcgct ggctgatttt aaatcgggtg
    10861 acattcgcgt actggtggcg accgatatcg ctgcgcgcgg tctggatatc gaagagttgc
    10921 cgcacgtcgt caactatgaa ctgccaaacg taccggaaga ttatgtccac cgtatcgggc
    10981 gtaccggtcg tgcggctgct accggtgaag cgttgtcgct ggtgtgtgtt gatgaacaca
    11041 aactgctgcg tgatatcgaa aaactgctga aaaaagagat cccgcgcatt gcgattccgg
    11101 gctatgagcc ggacccgtca atcaaagccg aaccgatcca gaacggtcgc cagcaacgtg
    11161 gcggcggcgg tcgtgggcaa ggtggtggtc gcggtcaaca gcaaccacgc cgtggggaag
    11221 gtggcgcaaa atctgcaagc gcgaaacctg cagaaaaacc gtctcgccgc ctcggcgatg
    11281 ccaaaccggc aggcgaacaa caacgtcgcc gccgtccgcg taaacctacc gctgcgcagt
    11341 aatcttttat gccgggctat gcccggcatc aggctgatga acaaacgcaa aactgcctga
    11401 tgcgctacgc ttatcaggcc tacgtgaact ctgcaatata ttgaatttgc atgcttttgt
    11461 aggccggata aggcgtttac gccgcatccg gcatttcaca acaagcactt gtcagcaatt
    11521 tgagaacacg ggaaaaaatt tatctgtttt accgcccata agccaccgag acgttacttc
    11581 tctatagcca attgttcgcg caactccatt aaaaggtagc ccagtcgatt cttgccctta
    11641 ccattaccac cgtctcccca gtaagcatcg ttttccgtat gctcaaccag ttttgcgggc
    11701 gcggttgcca gcaagagcgc acgcagttct ggatgctgtt cgaatttagc gcgaagtgct
    11761 tttcgcatca cctgttcttt gaccgactcc cagtttttac gcagaggctt agaacgatcg
    11821 cgccccatgc gtgcggcgac cataggggaa gaaacccgac ggatctcttc gcggtatttt
    11881 tcatcaagga atttttgtgc ctgaaaatag tgttctgagg tgggccaggt tttcccgtca
    11941 accttgatgg gccaggcggc aaagttagaa aaatcaccgt agtcatcgct ggtgctgtag
    12001 aaatttatga tggtgtcttg catgacgtgt tggatccttt gtgctcgaac gggcattaaa
    12061 ccgcattatg ttggtggtta ttgcgagccg ctttccagaa acagaaaaac cattacccct
    12121 gaaaaccgaa aaatgccaca atattggctg tttatacagt atttcaggtt ttctcatggc
    12181 attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc aggaacagat
    12241 ccccgacttt attccccgtg cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct
    12301 ggccggagaa gaagggcggc atctggcgat tgaagccccc accggcgttg ggaaaacgct
    12361 ctcctatttg atccccggca tcgccattgc ccgcgaagag caaaaaacgc tggtggtgag
    12421 taccgccaac gtggcgttgc aggatcagat ttacagcaaa gatttaccgc tgctgaaaaa
    12481 gatcattccc gatcttaaat tcactgccgc ttttgggcgt gggcgctacg tttgcccgcg
    12541 taatctgacg gcgctcgcca gcactgagcc cacgcaacag gatctgctgg cgtttcttga
    12601 cgacgaactg acgccaaata atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga
    12661 tctcgacact tataaatggg atggtctgcg cgatcatact gatatcgcta tagatgacga
    12721 tctctggcgt cgtttaagta ccgacaaagc cagctgcctc aaccgcaact gttactacta
    12781 tcgtgaatgc ccgttttttg ttgctcgtcg ggagattcag gaagcggaag tggtggtggc
    12841 aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc gtactacctg acccgaaaaa
    12901 tctgctgctg gtgctggacg aaggtcatca cctgccagat gtggcccggg atgcgctgga
    12961 gatgagtgcc gaaatcaccg cgccctggta tcgactacag ctggacttgt tcacgaaact
    13021 ggtcgctacc tgcatggagc agtttcgccc gaagaccatc ccaccgctgg cgatccctga
    13081 acgtttgaat gcgcattgtg aagagttgta tgagcttatc gcctcgttaa acaacattct
    13141 caatctctac atgcctgccg ggcaggaggc agagcaccgt tttgcgatgg gcgaactgcc
    13201 tgatgaagtg ctggagatct gccagcggct ggcaaaactc accgagatgc tgcgtggtct
    13261 ggcggagtta tttcttaacg atttaagtga gaaaaccggc agccacgata ttgtgcgtct
    13321 gcatcggttg attttgcaga tgaaccgcgc gttggggatg ttcgaggcgc aaagcaaact
    13381 ctggcggctg gcgtctctgg cgcaatcttc cggtgcgccg gtgaccaaat gggcgacgcg
    13441 ggaagagcgc gaagggcagc tgcatctctg gtttcactgc gtggggatac gtgtcagcga
    13501 tcagctggaa aggctgctgt ggcgcagtat tccgcacatt attgtcacct ccgcaacctt
    13561 gcgttcgctg aacagttttt cgcgtttgca ggagatgagc gggctgaaag agaaagcggg
    13621 cgaccgtttt gtggcgctgg attccccctt taaccactgc gaacagggca aaattgttat
    13681 tccccggatg cgctttgagc cttccatcga caacgaagag cagcatattg ccgaaatggc
    13741 ggcctttttc cgtgagcagg tggagagcaa aaaacatctc ggtatgttgg tactgtttgc
    13801 cagcggacgg gcgatgcagc gctttctcga ctatgtgacg gatttacgcc tgatgctgct
    13861 ggtgcagggc gatcagccgc gttatcgctt agttgaactg caccgcaaac gcgttgccaa
    13921 cggtgagcat agtgtgctgg tgggcttaca gtcatttgcc gaagggcttg atttgaaagg
    13981 tgatctgctc agccaggtgc atatccacaa aatcgctttt ccgcccatcg acagcccggt
    14041 ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc tatccgtttg aggtgcaaag
    14101 cctgccgagc gcctcgttta acctgattca gcaggttggg cgactgattc gaagccacgg
    14161 ttgctggggc gaagtggtta tctacgacaa acgcttgctg accaaaaatt atggcaagcg
    14221 actactggat gcattaccgg tatttccgat agagcaaccg gaagtccctg aaggtatagt
    14281 taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg
    14341 taaggagtcg taaatggact atcgcaaaat cattaaagag atcgggcgcg ggaaaaacca
    14401 cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat gcccatatgc tcaatggtga
    14461 agtccctgac cttgagttgg gcggcgtatt gattgcgcta cgtatcaaag gggaagggga
    14521 agcagagatg ctcggctttt acgaagccat gcaaaatcac accatcaagc tgacgccgcc
    14581 tgcaggcaag ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta aacaggccaa
    14641 cctgacgccg ttgttggcga ttctcctgca caaactcggt tttcctgtgg tggttcacgg
    14701 ggttagcgaa gatccaaccc gcgtgctgac tgaaaccatt tttgaattga tgggtattac
    14761 gccaacgttg cacggcgggc aggcgcaggc gaagctcgac gaacatcaac cggtgtttat
    14821 gccagtcggc gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct ggcggatggg
    14881 cgtgcgtaac agtgcacata ccctggcgaa actggcgacg ccatttgccg aaggtgaggc
    14941 gctgcgtctt tccagcgttt cgcatccgga atacattgga cgcgtcgcga agttctttag
    15001 cgatatcggc gggcgggcgc tactaatgca tggtactgaa ggtgaagtgt atgctaatcc
    15061 gcagcgctgc ccgcagatca atctcattga ccgtgaaggg atgcgggtgc tgtatgaaaa
    15121 acaggaccct gctggtagcg agttactgcc acaagcaaaa gatccggaaa ccacggcgca
    15181 gtggattgag cgttgccttg ctggcagcga accgattccc gaatcgctga aaatccagat
    15241 ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc agcgacggcc tggcgcgcgt
    15301 taatcaggca ttttaattct tttctcagcc ggatgagcca tgtcatccgg cttttacccc
    15361 accgcaatat gaaattcctg catctttatt gaccttccca cgcccggcgt gcagcataaa
    15421 aaaacaacaa acacataaca taaacaggag ttaaccatgg aaagtggtca tcgctttgat
    15481 gctcagacgc tgcacagttt tattcaggct gtatttcgtc agatgggtag cgaggaacaa
    15541 gaagcgaaat tagtggccga tcatttaatc gcggcaaacc tggcagggca tgattctcat
    15601 ggtattggca tgatcccaag ctatgtgcgt tcctggagtc aggggcatct gcaaattaac
    15661 catcatgcca aaatcgttaa agaggcgggg gcggcagtca cgctcgatgg cgatcgcgca
    15721 ttcggtcagg tcgcggcaca cgaagcgatg gcgctgggga ttgagaaagc gcatcagcac
    15781 ggtattgctg ccgtggcgct acataactcg catcatatcg gccgtatcgg ttactgggcg
    15841 gagcagtgtg cagcggcggg gtttgtctct atccactttg ttagcgtggt cggtattcca
    15901 atggtcgcac cgttccacgg tcgcgacagc cgctttggca ccaatccgtt ctgtgtggtt
    15961 ttccctcgta aagataattt cccgctgttg cttgattacg ccaccagcgc cattgcattt
    16021 ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg cctgattgac
    16081 gttaacggcg tgccgacgac caatccggcg gtaatgcagg agtcgccgtt gggttcgctg
    16141 ttgacctttg ccgaacataa aggctacgcc cttgcagcga tgtgtgaaat tcttggcggg
    16201 gcgctttccg gcggtaaaac gacgcatcag gaaacgttac aaaccagtcc cgatgccatt
    16261 cttaactgca tgaccactat catcatcaac ccggaactct tcggcgcgcc ggattgtaac
    16321 gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga tgataagccg
    16381 attttgctac cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagaa gcaggggatt
    16441 ccactggatg cgggaagctg gcaggccatt tgtgatgcag cgcggcagat tggtatgccg
    16501 gaagagacgt tgcaggcttt ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg
    16561 gcggacgggc aaacaagggt aacataggat caatgagggt tagagcatat gcgtctgtcg
    16621 gcaaacagac agggaaatac ttgtgctgga cgtagcgtaa acgcctgatc cgtccaacgg
    16681 ttcggatttt gtaggcctga taagacgcgg tagcgtcgca tcaggcatct gttgccggat
    16741 gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa attaccgcag taccgcttaa
    16801 tttattgttg ttggtggcgg aagtgatgct gtatccactg gcaccggctg ccgcggcttt
    16861 ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc
    16921 gattttattc atgttctgtg cctgggatgc cgttaccggt tccgcggcaa acacgccaaa
    16981 tgacagagtt gaaagagcca tagcagcaac aacagtattg atagttttca taattaatct
    17041 ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt
    17101 tttacgagag ataaaatcga agagaattga ccgccttgtt caaataaatt gattgatatc
    17161 taacatactg ataaaccatt tatttgttgt acttatggac ttgttttatg ccgcgtggca
    17221 gtcacagcag cgttaaaggc acggaatgcc acgcaaaata caaattatac tcgctatgca
    17281 tctcgccagt tgtagttaac gtaactggct gtctttcgaa cctctgcgat tatatcctgt
    17341 atacgctggt ttttgtaaat ctttctcctg ctgacaatga atgcataagc gcacgccagg
    17401 aatggcttcc cgacgggctt gcgggatggg ggcaccgcac tcttcacatt catccaggct
    17461 ttcgccacgc ggaatttcac cccgagcgcg ggcaatcgca tcttcaattg tactgttgat
    17521 ctgttcgttg acggcgtcat cgttagccca accggatgcc atatcgtcct ccccatatca
    17581 atgcttgtac agttaagtgt agctaatcca gggacgaact tgggcagttc aagcatcaga
    17641 tctccgacca ttcccgcagc agattatgat aaagattaag cagcgacagg atctcttcac
    17701 tttcgccgta gcggcttttc agcgactgaa tattgttgtc cagttcaaac agcatggcgc
    17761 gctttttatc atcacggatc atcgactgga tccacataaa tgatgccact cgtacgccgc
    17821 gggttacggg tgtcacgcaa tgcaggctgc tggagggata caacacgaga tcgcctgccg
    17881 ggagttttac ccgatgttgt ccgaaggtgt cattaacgac cagttcgccg ccgtcgtagc
    17941 tttgtggatc gcttaaaaac agcgtggcag aaaggtcagt acgcatccag ccgttttgtg
    18001 gatggctgcg tactgcgcca tccacatgga aaccataggt ttcattgttc tgatagcgat
    18061 taaacagcgg cgtggaaagg gtacgcggca aggccgcggc aaagaacaaa gcatgttggt
    18121 taaccgcgtt cagcacctca ttttgcaggg cggcgtataa cgtgctgcgg gtgtcgacct
    18181 gttgattgtt cttaacttgc gcaccttgtg cgccggtggt gacgcgtcca tccacccatt
    18241 cggcttgttc cagttgttcg cgaaaccggg cgacgtcctg tggcgataac acgccgggaa
    18301 tgtggtacat catacaagtt ctccaaaaag tggggcctgc gccccacatc tgaatcagaa
    18361 atgcatattg gctgtgagca agaaggttct tggctcgccc gggtgataac ggtagccgct
    18421 cttgttgatt gaggcgacgt aatcggtatc aaacaggttg taaacgttta gctggaagtc
    18481 gagattgcga ttaacccgat accccagttt ggcatcggcg acccagtaac cttcggtaaa
    18541 cgctggcgtt cccaccgcgc cgtctgaacc tttatgcata ctgccgatat agcgtgcgcc
    18601 cgcgccaaca gagatatcat cggttgcctg atactggctc cataaggtga aggcgtgctc
    18661 cggggtatac ggcagcgatg aggaaccatc ctgggcaaca tctttgccgt ttttgatggt
    18721 tgctttttgc tgggtatagc cgccaatcac ctgccacgcg ggagtgatat tcccggccac
    18781 ggatatctca tagccttcga cacgtttctt accgtattgc gagtaagttc cgtcatcatt
    18841 ttgctcaact tcattttcga tatcagtacg gaacagcgcg gcggtgagca acaggcgttt
    18901 atccagaacc tgccatttgg tgccaatctc gctggtgttg gctttttgcg gtttaaaatc
    18961 ggtgcggttg gcactgttac cgctgccaga ctgcgcaagg gcgaagttgt tgccgcccgg
    19021 aggctgctgg gaaacggcat agttaatata gacattaccg ttttccgtca ggtgatacag
    19081 cgccccggct ttccagttca ccagattgcc cgacttggcg gtgtcgacgg tggtgaccgg
    19141 agaacctttt gccacaccag ctgggcaggt gatggcaccg cgtccgctgc cgccgcaggc
    19201 ggtggcactg tcatattcag tatgataatt atccagacgg atcccgccgt tcagctcaaa
    19261 atcacgggtg atttgcagcg tatcgaaggc gtaaattgcg aaggtatccg tctgaccatt
    19321 ggcgtttgcg ccgttgcgcg tcaggccgcc gggatgaatg ctgctgtcag gatgataaat
    19381 atttaccgcg ggtaacgtca ccggattaac gccgtagtta gtctgcgttt cacgggtaaa
    19441 ttccacgccg gtactgacat catgaccgat agaaccggta tagaacgtcg aggtcaggtt
    19501 ggtctggttg gtgagaattt tattactcac atctttggta ttcgccgtgc gtgaccaggt
    19561 ccagctattc acatcgctgg tgggctgagt aatattcgac gccccgccca taatcgccgt
    19621 catcaggtaa tcctgcttta cgcgcgacca acgggtagta ttgcgaatgg tggtgttatc
    19681 gttgatgtcg tgctcaaaac gcatggtggc ggtgtcggtg gtcgaatcgt cgtaatcgga
    19741 atccgtgccg taaaagttat gagtatcaac ttttccggaa tgattcagag ccgctgttcc
    19801 cgcagatggg gcagaataac ccggcaaacc gatggtcgga atgccgccgt ctggcgtgtt
    19861 gtgctgggtg acatgcagat aattaagata caaacgattc gctgtaccaa ggccaaaagc
    19921 gatagaaggg gcgacgccgt aacgctcatt tttgactttg tcgcgtccgg catcgtgcgt
    19981 tttttcgccc atcacattca ggcgcaccgc agtggtatca ccaatgacct gattgacgtc
    20041 cagcgtgccg cggcggaacc aggcgctgcc aatactggcg gaggcgtcaa tgccggaatc
    20101 attgcgcggc tgcttgctga tcatattgat cgagcccgtc ggcgcgctgc gcccgtagtc
    20161 ggtgccggaa ggccctttaa tcacttcgac ctgctcagta ttgaaggtgt cgcgcgagac
    20221 gctgccgata tcgcgaatgc catcaatata aatactgtta gaggtatcag caccgcgcat
    20281 ataaatggcg tcgccagtgg tggagttacc gttctcaccc gcaaaaaacg cacccacgcc
    20341 ggggacgttt ttgagcgcgt cggtaaggtt ggttgcgccc tgatctttaa tcacttgttc
    20401 agaaatcacc gtcatcgtgc gggtagtatc cgctaccgga cgcgagaatt tcggatcggc
    20461 agattgttgt ggcgcataaa gcgaaggcgt tgatgcttcg acaaccagcg tgtcatccgc
    20521 gttagtttgc ccttcggcgg cgagtgcctg agccacaggc gtgatgccaa tacaaagacc
    20581 ggcaaagaac gtgagcgaat gaaattgtct ggcagggaaa ttgcgattgt tttccatttt
    20641 gcaggtgact tttttttata tgttgaaatt ttcactgcca cgagaaagcc tctctgcgga
    20701 tgcgatgcca ctgttaatct cgtgatagtg ttcgtgcaag tgataataat tttgataagc
    20761 attatcactt ggcgcgattt tctatcaccc attgcgcgaa aaagaaatac atttatttac
    20821 attgcgttca tatttgatgt tttttgatta caaaacaggc gaaaactgca tccagaggcg
    20881 aatgcagtta cgttggtggg gttatttgta gatggttgca gtaccgaaca tctggtcatt
    20941 tccgcctgcg gaattaatga cataaccttt cgcgccttgc tcgcgcgctt tttctgccag
    21001 tttatcctcc agatcgctta ggtttgatgc gccagttgcc gaaacggtac cggcgggccg
    21061 tagctgaccg ctgtccaggt tactcatcgg ttgcgcggca taagccgtga gagagattcc
    21121 gctcaggacg gtggcaatca gtagtgtgag gcactttttc ataatgacat cctcataaat
    21181 acaacgggta gtgcctgata agtgtaggtc gcctgggcga tgaaaatgac gcaaaaaatt
    21241 gacagagatg tgcttatttt ttgaacgata tatttttaca aaataagaca aatcaatgac
    21301 atgcataatg ctgattttta tgatgaattg ccataaacca tagccatgtt aaggttttgg
    21361 ggaatgataa acgcagccag atatttaccc tgtcgcgtta tgcgggtatc ttacgccgct
    21421 gcttaaagga gaatgctatg tccgcccaga aaccggggtt gcatccgcgc aaccgtcatc
    21481 acagccgcta cgatctcgcc acgctttgtc aggtcaatcc tgaactcagg caattcctca
    21541 cgcttacacc cgcaggggag caaagcgtag actttgccaa tccgctggcg gtgaaggcgc
    21601 tcaataaggc attgctggcc catttttacg ccgtagcgaa ctgggatatc ccggacggtt
    21661 ttctctgccc gccagtaccg ggccgggcgg attatattca tcaccttgcc gatttactgg
    21721 cagaagcaag cggaacaatt ccagctaatg ccagcattct ggatatcggc gttggtgcga
    21781 actgtattta tccgctgatt ggcgtacatg aatatggctg gcgttttacc ggtagtgaaa
    21841 ccagcagtca ggcgttaagt agtgcgcagg cgatcatcag tgctaatccg gggcttaacc
    21901 gcgccattcg tctgcgtcgg caaaaagaga gtggggcgat ttttaacggc atcatccata
    21961 aaaacgagca atacgacgcg accttgtgta acccgccatt ccacgattcc gccgctgcgg
    22021 cacgggcagg tagtgagcgt aaacgccgta acctggggct gaacaaagac gatgcactga
    22081 actttggcgg ccagcaacag gagttgtggt gtgaaggtgg tgaagtcgcc tttatcaaaa
    22141 agatgattga agagagcaaa ggcttcgcga agcaggtgat gtggtttacc tcgctggttt
    22201 ctcgtggtga aaacttaccg ccgttgtatc gtgctctgac ggacgtgggc gcggtgaagg
    22261 tggttaaaaa agagatggcc caggggcaaa agcagagtcg ctttattgcc tggaccttta
    22321 tgaacgacga gcagcgccgc cgttttgtta atcgccaacg ttaaaacgtt ggctcccccg
    22381 gcggcagcgg ttcagccggg gccgcgcccg gagcaggcag cacctgataa gtctgcactg
    22441 gcgcgcgaac gcccgccagg tcgaaatgtt ttttcacctg gctgtcgagg gcaaagcgta
    22501 ccgtccactg tttgagtggc agcgtggtga acgaaacacg cagtgtaaac gcggtattgc
    22561 ttaagccgac aatcccggca aaattcggtt caccaataat cagcccgcga atttcttcat
    22621 tttccatcaa ttccgctacc gcatctttca gtgcctgatt ggctttatca gcatcttcat
    22681 ggcgatcaac atcgtagttt gccactaccg aaccaatacc gcggacgaag ttagcaaagg
    22741 tggttatcga agaccacgga atgatgtgat acgccccggt atcctgacgc acgcccacgg
    22801 agcgaatcga catccgttcc acagtgccgg tcaacggccc gatagtaacc aaatctccag
    22861 tgttcatgcc gttttcaaac tgaataaata ccccggtgat aatatctttc accagcgttt
    22921 gcgaaccaaa cgagatagcc agacctaatg ccccagcacc tgccagcaat ggcgcgatat
    22981 tgacgccgat ttccgacaac acaatcatga tggtgatggt actgataatc accgccagcg
    23041 cgttacggaa cagcgtcagc agggtacgcg tacgggcgct gggtagcggg cggccatgaa
    23101 tatccgaagc cagccggttt tcgatcaaac tggcgagcac tgtccagcca accgccgaga
    23161 agaaaagaat gagtgcgatg cggatcagga tatctacggt tttctgcccc gcgccgtttt
    23221 gcagccagtt ccagaaatcg aacaatcccc atgcgctcaa cagcaacatt accgccacgc
    23281 agacggtcag aatacgcgcc gttttcagcg ctgccgaaag ccagccattc agccgttttt
    23341 gtagctccgg atagttacgc tgagtatgtg gcgagagggt gatggttttc gccagccagc
    23401 gggaaaacat accggaaaca aacgctgcga taccaataat cgccaggctg cgcaccgttg
    23461 cacccatcat aaatttcagg ctgttgcccg gatcgaacaa cgaaaagaaa aacagcacga
    23521 taaaataggc gcttgccagc cagtgccaca ccagcgcaaa ggcgcggata aacaggctga
    23581 aaaaggccag cgaatgctcc gcaaagttga gcaaatgttg ggtaatctct tttttattgc
    23641 gaaagatcag gtacaacgcc cagacggtca tgcacagcat aatgatgacg ttcgccagcg
    23701 cacctatctg tacattcacc tgattagaga taatcggcac ggccacaatc aggccataac
    23761 ctatcaaact gcttaaccag ctcaggcgac gactccagta acgggcgctc tcgtcctgaa
    23821 tcgtgaacgg gcgcagttca gccacgtttg ggcaaaaaat caggcgtagt acggctttga
    23881 aaaattcaat cagggcaaag gcattgagaa acaaactttg ttggaaagcg atggtgcgac
    23941 tgcctgcatt caggttgtcg cttaatacct ggccgacaaa taatgtcagg gccagtaaca
    24001 gcaggtcgat aataaacgcc ccgataatca tcgccggaag ctgcaaccag ttgctgcgct
    24061 cacgattttt ttgcctcgcc cactgaccca ttttgcgata cagcggcagt gcgcacaggc
    24121 gaatcagcca gtaaaaacca aacactaata ccgctaacat tgaaaaatgg ctcagcgcat
    24181 tgctgaaggt ttgtggatta aatggcttat gcggggagcc ggtgatattg cgataaagtt
    24241 gtccgaagcg ggcggaaagg gcttcaccat aatggcggct gacttcggtg accttttgca
    24301 gcacggtttg ctcttcaacc agcgtcggcg gcacgatctt cgggaccggt tccgcagggg
    24361 gcgtcgcggc gacagtacgc aactggtcga tcaattcttt acgcgaggtg tcgttatcca
    24421 gcacatccgc cagtgcgcca taggccgctt ttttttgttc gatatccggt tccggggccg
    24481 gttcagtcgt tgagtccgtt gtcgttgtgg ttgtaacgcc gggtatggat accgcgtggg
    24541 caggtgcgcc cagcaggcag aagaggatga acaggatcca ccgcatgact cctccagtga
    24601 gaaaacagag caaaaagata agtatagatg ctgggggagg ggcgatttta aatgagagga
    24661 atctggtgtg cctccctttc gggtgaaagg gaggcagaga agttaagaga cgtgctgtaa
    24721 aaattcctgc aagcgctggc tcggcgggtt cttgatcaat acctgcggat cgccatcttc
    24781 cgcaatccgg cctttgtcga taaagatcag ccgcgaagct actttctcgg caaaaccgat
    24841 ttcgtgggtc acgatcacca tcgtcatccc ttcttcagcc agatcctgca taaccttcag
    24901 cacttcatgg cgcagttccg ggtcaagagc ggaagtcggt tcatcaaaca gcatcatttt
    24961 cggcttcacc gccagcgcgc gggcaatcgc cacacgctgc tgttgaccac cagaaagttc
    25021 ggaagggtag tgatgtgcac gttctgccag accgactttc gccagcagct cacgcgccag
    25081 tttttccgcc tcttctttgt tcgcgccacg cacgcgtagc ggaccaaaca tgacgttttc
    25141 cagcgccgtc agatgcggga agaggtaaaa ctgctggaac accatacccg cttcctggcg
    25201 aatcaggcgc tcgtcaactt tcggatcgtt aaccttcagg ccatcgacaa tcagatcgcc
    25261 ggaggtgatt tcttccagtt tgttgatgca gcgcagcagg gtcgatttac cggaaccgga
    25321 cggcccgata atcaccacga cttcgccctg agcaatgttc aaatcgatat tgtgcagcac
    25381 ctgggttgga ccaaaatgct tggagacgtt tttaaattca atcacaggat tttcatcctt
    25441 ctttccagac gacgcagaat aaagctcagc accagggtaa taatcagata gaacaccgcc
    25501 acggcgctcc agatctcaag ggcgcggaag ttaccggcaa taatttcttg cccctgacgg
    25561 gtcagttccg ccacgccgat aacaataaac agcgaggtgt ctttaatgct gatgatccac
    25621 tggttaccca gcggcggcag catacgacgc agtgccagcg gtaaaatgac gtagcgaatg
    25681 gtttcccaac gtgaaagacc gagcgccagt cctgcttcac gaaaaccttt gtgaatagac
    25741 agcaccgcac cacgcgtgat ttccgcaatg tacgcgccgg agttgatcat gatggtgacc
    25801 accgccgcag taaatgggtc gatgcgtaag tcgttaaacg ccatcggcag ggcgaaatag
    25861 ataaacatca cctggacgac gataggtgtg ccgcggatca cttcaataaa gaccagcgcg
    25921 acgtggttgg ctatccaacc accgaaggtg cgtgcaaaac ctgccagcaa tccgattacc
    25981 agaccgcctg ccagaccgag gaccgaaatc cacagggtca ttttggcacc ttcaatcaga
    26041 agcggaatgg caggccagat ggcactccag tcaaactgca tatgttgttc ctgttaccgt
    26101 ggtgtaaata atcaaattca gggtaattaa tggccccgga gcgggtttgc gcccctcacc
    26161 ctaatcctct ccccataggg gagagggaac tgccagtgcg ttttacaggt gtagcgttat
    26221 tatttcggtt cagtaccgaa ccattttttg tagatttcgt tgtaagttcc gttctcgcgc
    26281 agggttttca acgcgccgtt gactttgtca cgcagctcgt cgctaccttt cgggaacgca
    26341 ataccgtatt gctgcgcttc cagagagtca cctaccgctt tgaactgacc gttaccggcg
    26401 gttttgatga agtacagaat gtttggcgta tcgtgcagaa cggcgtctgc gcggttggtg
    26461 cccagttcca tataggcgtt atcgatgttc gggaactgac gcagatcttt agttttgatg
    26521 tttgctttcg cgtaatcaac ggagccagta ccgctcttca cagcaaccac tttcccgtcg
    26581 agatctttca cgcttttcac atcgttattg ttagctttca ccatcactaa caggccgctt
    26641 ttgtagtagc cgtcagagaa atcgatcgct tttttacgct cgtcggtgat ggtaatgccc
    26701 gccagcgcca gatcgacgtt tttggtttgc agtgccggaa tgatcccact gaaatccatc
    26761 ggcttcagtt cgtaatccag cttcagctct ttagcgatgg cagcccacag atcaacgtca
    26821 aagcccacat atttatcgcc ctgtttaaat tcaaacggaa cgaaggcggt atccgtcgcg
    26881 acaactaatt ttttatccgc ggcatgagaa gaaaccgcaa aagccagggt cagtgcagcc
    26941 agtgaaactt ttaatacaga cttcatagca tttccttttt tctatccacg ggacgatccc
    27001 ctgcgtgaca ttcacatata tgaaaaaatc gtgccagttt tgcaactcct tgatttttcg
    27061 agatgcggca aaaacgtgat gcacgattta tagggcaata ccctgaagat gcaccattct
    27121 ggggcaccaa tctggtgcgc taaaattgtg cactcaacac agtgattatt tagcgtaaaa
    27181 cctgatgaaa aaacaatctt tatgtaacga ttgtgtgatg atgtggatac aaaaaattta
    27241 cttaatcagc tggagatagc agatggatgc actaaataag tgcgttgagg tgggctgcaa
    27301 aacaaaacgg cctcctgtca ggaagccgct tttatcgggt actaaagttc tgcaccatca
    27361 gcgatggatt tattcgatgt tagactcgat aaaccacagg aatttatcca ggtcgcgaga
    27421 cgcggcggtc aggatatctg cggtgtcgtc atctttcgct tcgccaatcg ctttgcgtac
    27481 gtcattagcg acgattgcgt aacggtcagc cagttctttc aggtgatcct gaacgttgtg
    27541 gatgtccagc gggtaacttt tcagcggggt tttgctgttg ataacttgag tggtccccag
    27601 agctacaccg cccagctgca ctgcacgttc tgccatggta tccagatgat cgatcagtgc
    27661 ggtgcggaag ccatccagca tttcatgtac ggcaatgaag ttagcgccgc gcatgttcca
    27721 gtgcgcttgt ttggtaatca aagaaagatc aataaactgg ataacctggc gattcagcaa
    27781 ctctactgtt gcttttttct cgctgtcgga gacatcgttg cgggtataaa gcagattggt
    27841 cgcttttgat ttaactaatt tagcggtact cataatttca tatcctcttg atgttatgtc
    27901 ccagtaatta acgagactaa gtatagcacc ggctatgtgt tccgctattc tggctgttcc
    27961 tatcacacta atagtggtaa caagcgtgaa aaacaaaact aataaagatt caatgagtta
    28021 gatatattga taagaaca
//
LOCUS       DAPPNJ010000044        27466 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000044 DAPPNJ010000000
VERSION     DAPPNJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.044"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(16..1266)
                     /gene="icd"
                     /locus_tag="RPM73_001518"
     CDS             complement(16..1266)
                     /gene="icd"
                     /locus_tag="RPM73_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDX6519891.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1438..2091
                     /gene="rluE"
                     /locus_tag="RPM73_001519"
     CDS             1438..2091
                     /gene="rluE"
                     /locus_tag="RPM73_001519"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDX6519892.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2101..2562
                     /gene="nudJ"
                     /locus_tag="RPM73_001520"
     CDS             2101..2562
                     /gene="nudJ"
                     /locus_tag="RPM73_001520"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDX6519893.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2616..3722
                     /gene="mnmA"
                     /locus_tag="RPM73_001521"
     CDS             2616..3722
                     /gene="mnmA"
                     /locus_tag="RPM73_001521"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDX6519894.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWNNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3758..4399
                     /gene="hflD"
                     /locus_tag="RPM73_001522"
     CDS             3758..4399
                     /gene="hflD"
                     /locus_tag="RPM73_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDX6519895.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4403..5773
                     /gene="purB"
                     /locus_tag="RPM73_001523"
     CDS             4403..5773
                     /gene="purB"
                     /locus_tag="RPM73_001523"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDX6519896.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5941..6612
                     /gene="phoP"
                     /locus_tag="RPM73_001524"
     CDS             5941..6612
                     /gene="phoP"
                     /locus_tag="RPM73_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDX6519897.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6612..8072
                     /gene="phoQ"
                     /locus_tag="RPM73_001525"
     CDS             6612..8072
                     /gene="phoQ"
                     /locus_tag="RPM73_001525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDX6519898.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8148..9269
                     /gene="roxA"
                     /locus_tag="RPM73_001526"
                     /gene_synonym="ycfD"
     CDS             8148..9269
                     /gene="roxA"
                     /locus_tag="RPM73_001526"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDX6519899.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9415..10644)
                     /gene="pepT"
                     /locus_tag="RPM73_001527"
     CDS             complement(9415..10644)
                     /gene="pepT"
                     /locus_tag="RPM73_001527"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDX6519900.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10894..12030
                     /gene="potA"
                     /locus_tag="RPM73_001528"
     CDS             10894..12030
                     /gene="potA"
                     /locus_tag="RPM73_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDX6519901.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVHERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12014..12871
                     /gene="potB"
                     /locus_tag="RPM73_001529"
     CDS             12014..12871
                     /gene="potB"
                     /locus_tag="RPM73_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDX6519902.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12868..13662
                     /gene="potC"
                     /locus_tag="RPM73_001530"
     CDS             12868..13662
                     /gene="potC"
                     /locus_tag="RPM73_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDX6519903.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13659..14705
                     /gene="potD"
                     /locus_tag="RPM73_001531"
     CDS             13659..14705
                     /gene="potD"
                     /locus_tag="RPM73_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDX6519904.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14763..15224
                     /locus_tag="RPM73_001532"
     CDS             14763..15224
                     /locus_tag="RPM73_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDX6519905.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            15221..16009
                     /locus_tag="RPM73_001533"
     CDS             15221..16009
                     /locus_tag="RPM73_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDX6519906.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16148..16969)
                     /gene="cobB"
                     /locus_tag="RPM73_001534"
     CDS             complement(16148..16969)
                     /gene="cobB"
                     /locus_tag="RPM73_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDX6519907.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16985..17896)
                     /gene="nagK"
                     /locus_tag="RPM73_001535"
     CDS             complement(16985..17896)
                     /gene="nagK"
                     /locus_tag="RPM73_001535"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDX6519908.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17925..19169)
                     /gene="lolE"
                     /locus_tag="RPM73_001536"
     CDS             complement(17925..19169)
                     /gene="lolE"
                     /locus_tag="RPM73_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDX6519909.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19169..19870)
                     /gene="lolD"
                     /locus_tag="RPM73_001537"
     CDS             complement(19169..19870)
                     /gene="lolD"
                     /locus_tag="RPM73_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDX6519910.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19863..21062)
                     /gene="lolC"
                     /locus_tag="RPM73_001538"
     CDS             complement(19863..21062)
                     /gene="lolC"
                     /locus_tag="RPM73_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDX6519911.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21324..22397
                     /locus_tag="RPM73_001539"
     CDS             21324..22397
                     /locus_tag="RPM73_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDX6519912.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22525..25971
                     /gene="mfd"
                     /locus_tag="RPM73_001540"
     CDS             22525..25971
                     /gene="mfd"
                     /locus_tag="RPM73_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDX6519913.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAERVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26115..27077
                     /gene="ldtC"
                     /locus_tag="RPM73_001541"
     CDS             26115..27077
                     /gene="ldtC"
                     /locus_tag="RPM73_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDX6519914.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27160..27417)
                     /gene="bhsA"
                     /locus_tag="RPM73_001542"
     CDS             complement(27160..27417)
                     /gene="bhsA"
                     /locus_tag="RPM73_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDX6519915.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6677 a         7120 c         6970 g         6699 t
ORIGIN      
        1 aaacacatgt agtgattaca tgttcttgat gatcgcatca ccaaattctg aacatttcag
       61 cagtttagcg ccttccatca gacgttcgaa gtcataggtt acagtcttgg cgttgattgc
      121 gccttccata cctttaacaa tcaggtctgc ggcttcagtc cagcccatat ggcgtaacat
      181 catctcagcg gagagaataa tagagccagg gtttactttg tcctgaccgg cgtatttcgg
      241 cgcagtaccg tgggtggctt caaacagggc gcattcgtca ccgatgtttg caccaggggc
      301 gataccgata ccgccaacct gcgctgccag ggcgtcagaa atgtagtcac cgttcaggtt
      361 catacaggcg ataacatcat attcagccgg acgcagcagg atctgttgca ggaatgcatc
      421 agcaatcacg tctttaatga cgatctcttt gccggtgttc gggtttttaa ctttcagcca
      481 cgggccgccg tcgatcagtt caccgccaaa ctcttcacgc gccagctggt agccccagtc
      541 tttaaacgcg ccttcggtga acttcatgat gttgcctttg tgcaccaggg tcacagagtc
      601 acggtcgttg gcaattgcgt attcaatcgc ggcacggacc agacgtttgg tgccttcttc
      661 agaacacggc ttgataccga taccgcaatg ttccgggaag cgaattttct tcacgcccat
      721 ctcttcacgc aggaatttaa tcactttctc ggcgtcggca gagtctgctt tccattcgat
      781 acccgcataa atgtcttccg agttttcacg gaagataacc atatcggtca gttcagggtg
      841 tttaaccggg cttggagtgc cttgatagta acgtaccgga cgcaggcaga tgtagagatc
      901 cagttcctgg cgcagggcaa cgttcagaga gcgaataccg ccaccaacag gagtggtcag
      961 cggaccttta atggcaacgc gatattcacg aatcagatca agggtttcag caggcagcca
     1021 gacgtcctga ccataaacct gtgtggattt ttcaccggtg taaatttcca tccaggagat
     1081 tttacgctcg cctttatagg ctttctcgac tgcagcgtcg accactttca gcatggctgg
     1141 ggttacatct acaccgattc catcaccttc aatgtaaggg ataatcggat tttcaggaac
     1201 gttgagtttg ccgttttgca gggtgatctt cttgccttgt gccggaacaa ctactttact
     1261 ttccattcac ctctccttcg agcgctactg gtttgctcgt ctgccaccac gttgcatatg
     1321 cgtttgcgtc ctgcgatacg gatgctttag agcaattttt tgttaatgat ttgtaattgg
     1381 cttgtcaata ctaccctatt gtttgccgtc atgaaagatg cgcgttatta ggctataatg
     1441 cggcaattca taatctctga aaataccatg caaaaaactt cttttagaaa tcaccaggtt
     1501 aagcgattca gctcgcaacg ttctaccagg cgtaaacctg aaaaccagcc cacgcgtgtg
     1561 atcctgttca ataaacccta cgatgttctt ccgcagttca ccgatgaagc cggacgcaaa
     1621 acattaaaag aattcatccc ggttcagggc gtttatgcag caggtcgtct tgaccgcgat
     1681 agcgaagggt tactggtgct gaccaataac ggcgcgttgc aggcgcgttt aacccagccg
     1741 ggtaaacgca ccgggaaaat ctattatgtg caggtggaag gtattcccac acaagacgca
     1801 cttgaagcct tgcgcaatgg cgtaacctta aatgatggcc ctaccctgcc cgccggcgcg
     1861 gagctggttg acgaaccagc gtggttatgg ccgcggaatc caccaattcg tgaacgcaaa
     1921 agtattccca ccagctggct gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc
     1981 cgcatgaccg cccatgttgg tttccccacg ctgcgactga ttcgctatgc gatgggagat
     2041 tactctttgg ataatcttgc caatggcgaa tggcgagaag tgacagatta aggatgcaaa
     2101 atgtttaaac cgcacgttac cgttgcttgc gtggtgcacg cagaaggcaa atttttagtc
     2161 gttgaagaga cgattaatgg taaagcgtta tggaaccaac ctgccgggca tctggaagcc
     2221 gatgaaacct tagtggaagc cgccgcccgt gaactgtggg aagaaaccgg catcagcgcg
     2281 cagccgcaac actttattcg tatgcatcag tggattgcgc cagataaaac gccgtttttg
     2341 cgtttcctct ttgccattga gcttgagcaa atatgcccga cccagcctca tgacagcgat
     2401 atcgactgct gtcgttgggt cagcgccgaa gagattttaa aggcgtcaaa tcttcgttcg
     2461 ccgctggtgg cggaaagtat ccgttgttat caaagcgggc aacgctatcc gctggagatg
     2521 attggcgatt ttaactggcc ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat
     2581 acgccgcctt gaagttcaat gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa
     2641 tcgtcggcat gtccggcggt gtcgattcct ccgtttctgc ctggctgttg caacaacagg
     2701 gatatcaggt tgaaggcctg tttatgaaga actgggaaga agacgacggt gaggaatatt
     2761 gcacagcggc agcggatctg gctgatgccc aggctgtctg cgacaagctc ggcattgaac
     2821 tgcacaccgt taactttgct gccgagtact ggaacaacgt cttcgaactg ttccttgccg
     2881 aatataaagc cggtcgcacg ccgaatccgg atattctgtg caacaaagag atcaaattta
     2941 aagccttcct cgaatttgcc gccgaagatt taggtgccga ttatatcgct accggtcatt
     3001 acgtacgtcg ggccgatgtc gatggcaaga gccgcctgct gcgtggtctg gacagcaata
     3061 aagaccagag ctacttcctt tatacgctca gccatgagca gattgcgcaa agcctgttcc
     3121 cggtcggcga actggaaaaa ccgcaggtgc gtaagattgc tgaagatctt ggtctggtca
     3181 ccgcgaagaa aaaagactct accggcattt gcttcattgg cgaacgtaaa ttccgcgagt
     3241 tcctgggccg ttatctcccg gcgcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa
     3301 ttggtgagca ccaggggctg atgtatcaca ctctcggtca gcgtaaaggt ctgggtatcg
     3361 gtggcaccaa agaaggtacc gaagaaccgt ggtatgtggt ggacaaagac gtcgaaaaca
     3421 acattctggt tgtcgctcag ggccatgaac acccgcggct gatgtctgtc gggttgattg
     3481 cccagcagtt gcactgggtc gatcgcgaac cattcaccgg cactatgcgt tgcacggtaa
     3541 aaacccgcta tcgccagacc gatatccctt gcaccgtcaa ggcgctggac gatgatcgca
     3601 ttgaagtgat tttcgatgaa ccggttgccg ccgtgacgcc gggccagtct gccgtcttct
     3661 ataacggtga agtgtgcctc ggtggcggta ttattgagca gcgtctgccg ctgccggtct
     3721 gattattatc tttacttaaa acaaggaagc agtgaacgtg gcaaagaatt actatgacat
     3781 caccctcgcc ctggccggta tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca
     3841 ggggcattgt gatgccgatg cgctacacgt ctcactcaac agtattattg atatgaaccc
     3901 cagctcgacg ctggcggttt ttggcggtag cgaagccaac ctgcgcgtcg ggctggaaac
     3961 cctgctcggc gtgctcaatg ccagcagtcg ccagggctta aacgccgaat taacccgcta
     4021 cacactcagc ttgatggtgc ttgagcgcaa actctcctca gcgaaaggcg cgctcgacac
     4081 tctgggcaac cggatcaacg gcctgcaacg ccagctcgaa cacttcgatt tacagtccga
     4141 aacgctgatg agcgcgatgg ctgctatcta tgttgatgtg atcagcccgc ttggcccgcg
     4201 cattcaggtc accggttccc ctgctgtact gcaaagccca caagtgcagg cgaaagttcg
     4261 cgcaaccctg ctggcaggca ttcgcgccgc cgtgctctgg caccaggtcg gcggcggacg
     4321 tctgcaactg atgttttctc gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca
     4381 tttaaccccg gagttgtgat ctatggaatt atcctcactg accgccgttt cccctgtcga
     4441 tggacgctac ggcgataaag tcagcgcgct gcgcggaatt ttcagcgaat atggtttgct
     4501 gaaattccgt gtacaagttg aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat
     4561 caaggaagtt cctgcttttg ctgccgacgc aatcggttac cttgatgcaa ttgtcgccaa
     4621 tttcagcgaa gaagatgccg cacgcatcaa aaccatcgag cgtaccacta accacgacgt
     4681 taaagcggtt gagtatttcc tgaaagaaaa agtggcggag atcccggaac tgcacgcggt
     4741 ttctgaattc atccactttg cctgtacttc ggaagatatc aataacctct cccacgcatt
     4801 aatgctgaaa accgcgcgtg atgaagtgat cctgccgtac tggcgtcaac tgattgatgg
     4861 cattaaagat ctcgccgttc agtaccgcga tatcccgctg ctgtctcgta cccacggtca
     4921 gccagccacg ccgtcaacca tcggtaaaga gatggcaaac gtcgcctacc gtatggagcg
     4981 ccagtaccgc cagcttaatc aggtggagat cctcggcaaa atcaacggcg cggtcggtaa
     5041 ctataacgcc catatcgccg cttacccgga agttgactgg catcagttca gcgaagagtt
     5101 cgtcacctcg ctgggtattc agtggaaccc gtacaccacc cagatcgaac cgcacgacta
     5161 cattgccgaa ttgtttgatt gcgttgcgcg cttcaacacc attctgatcg actttgaccg
     5221 tgacgtctgg ggttatatcg cccttaacca cttcaaacag aaaaccattg ctggtgagat
     5281 tggttcttcc accatgccgc ataaagttaa cccgattgac ttcgaaaact ccgaagggaa
     5341 tctgggcctt tccaacgcgg tattgcagca tctggcaagc aaactgccgg tttcccgctg
     5401 gcagcgtgac ctgaccgact ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc
     5461 gctgattgcg tatcaatcca ccctgaaagg cgtgagcaaa ctggaagtaa accgtgacca
     5521 tctgctggat gagctggatc acaactggga agtgctggct gagccaatcc agacagttat
     5581 gcgtcgctat ggcatcgaaa aaccgtacga gaagctgaaa gagctgactc gcggtaagcg
     5641 cgttgacgcc gaaggcatga agcagtttat cgatggtctg gcgttgccag aagaagagaa
     5701 agcccgcctg aaagcgatga cgccggctaa ctatattggt cgagctatca ccatggttga
     5761 tgagctgaaa taaactcgta tcagtgccgg atggcgatgc tgtccggcct gtttattaaa
     5821 attatccact ttttcttttt tcactttacc tcccctcccc gctggtttat ttaatgttta
     5881 cccccataaa cacataatcg cgttacacta ttttaataat taagactggg agaaataaaa
     5941 atgcgcgtac tggttgttga agacaatgcg ttgttacgtc accaccttaa agttcagatt
     6001 caggatgctg gtcatcaggt cgatgacgca gaagatgcca aagaagccga ttattatctc
     6061 aatgaacatt taccggatat tgcgattgtc gatctcggat tgcctgacga ggacggtctg
     6121 tcactgattc gccgctggcg tagcaatgat gtttcactgc cgattctggt attaaccgcc
     6181 cgtgaaagct ggcaggacaa agtcgaagta ttaagtgccg gtgctgatga ttacgtgact
     6241 aaaccgtttc atattgaaga ggtgatggcg cgaatgcagg cattaatgcg gcgtaatagc
     6301 ggcctggctt cacaggtcat ttcgctaccc ccgtttcagg ttgatctctc tcgccgtgaa
     6361 ttatctatta atgacgaagt gatcaaactg accgcgttcg aatacaccat tatggaaacg
     6421 ttgatacgca ataatggcaa agtggtgagc aaagattcgt taatgctcca actctatccg
     6481 gatgcggagc tgcgggaaag ccataccatt gatgtactga tgggacgtct gcgcaaaaaa
     6541 attcaggcac aatatccgca agaagtgatt actaccgttc gcggccaggg ctatttgttc
     6601 gaattgcgct gatgaaaaaa ttactgcgtc tttttttccc gctctcgctg cgggtacgtt
     6661 ttctgttggc aacggcagcg gtagtactgg tgctttcgct tgcctacgga atggtcgcgc
     6721 tgatcggtta tagcgtcagt ttcgataaaa ctacgtttcg gctgttacgt ggcgagagca
     6781 atctgttcta tacccttgcg aagtgggaaa acaataagtt gcatgtcgag ttacccgaaa
     6841 atatcgacaa gcaaagcccc accatgacgc taatttatga tgagaacggg cagcttttat
     6901 gggcgcaacg tgacgtgccc tggctgatga agatgatcca gcctgactgg ctgaaatcga
     6961 atggttttca tgaaattgaa gcggatgtta acgataccag cctcttgctg agtggagatc
     7021 attcgataca gcaacagttg caggaagtgc gggaagatga tgacgacgcg gagatgaccc
     7081 actcggtggc ggtaaacgtc tacccggcaa catcgcggat gccaaagtta accattgtgg
     7141 tggtggatac cattccggtg gagctaaaaa gttcctatat ggtctggagc tggtttatct
     7201 acgtgctctc agccaatctg ctgttagtga tcccgctgct gtgggtcgcc gcctggtgga
     7261 gtttacgccc catcgaagcc ctggcaaaag aagtccgcga actggaagaa cataaccgcg
     7321 aattgctcaa tccagccaca acgcgagaac tgaccagtct ggtacgaaac ctgaaccgat
     7381 tgttaaaaag tgaacgcgaa cgttacgaca aataccgtac gacgctcacc gacctgaccc
     7441 atagtctgaa aacgccactg gcggtgctac aaagtacgct acgttctctg cgtagtgaga
     7501 agatgagcgt cagtgatgct gagccggtaa tgctggagca aatcagccgc atttcacagc
     7561 aaattggcta ctacctgcat cgtgccagta tgcgcggcgg gacattgctc agccgcgagc
     7621 tgcatccggt cgccccactg ctggacaatc tcacctcggc gctgaacaaa gtgtatcaac
     7681 gcaaaggggt caatatctct ctcgatattt cgccagagat cagctttgtc ggtgagcaga
     7741 acgattttgt cgaggtgatg ggcaatgtgc tggataatgc ctgtaaatat tgcctcgagt
     7801 ttgtcgaaat ttctgcaagg caaaccgacg agcatctcta tattgtggtc gaggatgatg
     7861 gccccggtat tccattaagc aagcgagagg tcattttcga ccgtggtcaa cgggttgata
     7921 ctttacgccc tggacaaggt gtagggctgg cggtagcccg cgaaatcacc gagcaatatg
     7981 agggtaaaat cgtcgccgga gagagcatgc tgggcggtgc gcggatggag gtgatttttg
     8041 gtcgccagca ttctgcgccg aaagatgaat aaatatgtcc atacttcacg cattacgtta
     8101 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
     8161 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
     8221 aacgcggctt taataatttt attgacccga tctctccaga cgagttggcg ggtctggcga
     8281 tggaaagcga agtcgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
     8341 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
     8401 cagtgaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
     8461 actggcgtat tgatgatctg atgatctctt tttctgtacc gggcggcggc gtcggcccgc
     8521 atctcgatca gtacgacgtg tttatcattc agggtaccgg ccgtcgtcgc tggcgagtgg
     8581 gcgaaaagct gcaaatgaaa cagcactgtc cgcacccgga tctgttacaa gtcgatccgt
     8641 tcgaagccat catcgatgaa gagctggagc ctggcgatat tctttatatt ccgccaggat
     8701 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
     8761 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
     8821 gcaactacta cagcgatccg gatgttccac ctcgcgctca tcctgcggat gttctgccgc
     8881 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
     8941 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccag
     9001 agccgcctta tcagccggat gaaatctacg atgcgctgaa acaaggtgaa gtgctggtgc
     9061 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
     9121 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgaccgcgg
     9181 agaattttgg tgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
     9241 atagcggata ctggttcttc gaaggataag tttagattta agccggatgc ggcgctgatt
     9301 gcgccttatc gtgcctacat gcactgaacg cctgtaggca cgataagtat gtggaccagt
     9361 atcaatataa cggtttcatc ccggtaattg gcagtccaca tacgcatcgg gcaatcacga
     9421 cttccgttgc gccgttaact cggcaatacg gacaatcacc tgcaccgctt tttccatacc
     9481 ttccagagtc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
     9541 gcacggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
     9601 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
     9661 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccagtt caatgtagca
     9721 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
     9781 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggctc ggtcaacggt
     9841 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
     9901 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
     9961 tttcgccgtg cccggatgaa cattgttacc gacaattttg atattgaccg atgcagcgtt
    10021 gaagttttca aattccagtt cgcctacgcc accaccatca acggtgtaag cccagcgggc
    10081 atcgaaggcg tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
    10141 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
    10201 gatttctgca atacctgctt tgtcatcggc acctaacaag gttttaccat cggtggtaat
    10261 cagcgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
    10321 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattcacatt
    10381 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
    10441 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
    10501 gataagcccc atctcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
    10561 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
    10621 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
    10681 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
    10741 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
    10801 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
    10861 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
    10921 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
    10981 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
    11041 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
    11101 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
    11161 tatgtgaaca ctgttttcca aagctacgca cttttccccc acatgaccgt gttcgaaaat
    11221 gtggcctttg ggttgcgtat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
    11281 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gcaaaccgca tcagctctct
    11341 ggtggtcaac agcaacgcgt cgccattgcc cgcgcggtgg ttaacaagcc tcgtctgttg
    11401 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
    11461 ctgaaagcgt tacagcgtaa gcttggcatt acattcgtct tcgtgactca cgaccaggaa
    11521 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
    11581 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg cttcattggc
    11641 gaaatcaata tgtttaacgc cacggtcatc gaacgtctcg acgagcagcg cgtacgcgcc
    11701 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
    11761 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
    11821 gctgaagggc tgattggtta cgttcacgag cgtaactaca aaggcatgac gctggagtcg
    11881 gttgttgaac ttgaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgac
    11941 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
    12001 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
    12061 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
    12121 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
    12181 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
    12241 cctcgcctgc ctggtgctgg gctacccgtt tgcctggttt ctggcgaagc tgccacacaa
    12301 ggtgcgtccg ctgctgctgt ttctgctgat tgttcccttc tggaccaact cattgattcg
    12361 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
    12421 gctgggcgtt atcgacacac caattcgtat catgttcacg cccagtgcgg tgattatcgg
    12481 tctggtttac attctgctac cgtttatggt gatgccgctg tactccagta tcgaaaaact
    12541 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
    12601 ccgtatcatt attccactga cgatgccggg aattattgcc ggatgtctgc tggtgatgct
    12661 gccagcgatg ggcctgttct atgtatccga cctgatgggc ggtgcgaaaa acctgctgat
    12721 cggtaacgtc atcaaggtcc agttcctcaa tattcgtgac tggccgtttg gtgcagccac
    12781 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
    12841 tctgctgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
    12901 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
    12961 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
    13021 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
    13081 acatttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgt
    13141 ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga tatcgtgatg
    13201 gcgatttctc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
    13261 ctgttctcgc atatcacctt ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg
    13321 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag tgaatttacc
    13381 attctgcgaa aaatcattct gccactggca atgcctgcgg tggcggcggg ctgggtgtta
    13441 agctttaccc tgtcgatgga cgatgtggta gtttcttcgt tcgtcaccgg accaagttat
    13501 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
    13561 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
    13621 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
    13681 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
    13741 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
    13801 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
    13861 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
    13921 ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
    13981 gcaatctcga tccagacatg ctcaacaagc cgtttgaccc gaataacgac tactccattc
    14041 cgtatatctg gggtgcgacg gcgattggcg ttaacggtga tgcggtggat cctaaatctg
    14101 tcaccagttg ggcagatctg tggaagccag agtacaaagg tagcctgctg ctgaccgacg
    14161 atgcccgtga agtgttccag atggcgctgc gcaaactggg ctactccggt aacaccaccg
    14221 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
    14281 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
    14341 tctggaacgg ttctgctttc gttgcacggc aggcgggtac gcctattgac gtggtgtggc
    14401 cgaaagaagg cggcattttc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
    14461 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
    14521 ccgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agcccggaag
    14581 tggcgaacga taaaacgctc tacccggatg ctgaaaccat taaaaatggt gaatggcaga
    14641 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    14701 gttaattcag caagcgggcg gtggcattac cgcccgtttt ttatttagca aggatgtggc
    14761 taatgagtca ggacagtaaa gttttatccc gcgcttttct cggtatcggt ctgatattgc
    14821 tcgttatttc tgcaatcatc ttttattacc attttacttt taccaaaagc gcggttcata
    14881 cagaaggaat cattgttgat gccgtttggt acaacaatca ctcaaacgac gtcgatgata
    14941 atggttcctg gtatccagtg gttgcctttc gccctacgcc agattacacg ctaattttca
    15001 attcgaatat cggtagtgat ttttatgaag atagcgaagg tgacagagtc aatgtctatt
    15061 atccgccagg gcatccagaa caagccgaaa taaacaatcc ctgggttaat ttttttaaat
    15121 ggggctttgt tggcatagca ggcattattt tcggtagtgt cggtctgata atcacgctcc
    15181 cctccaccaa aaaatctcgc aggaaacgga agtcctgccc atgaaaaaat tcattatact
    15241 gctcagttta ctgatactcc tgcctctggt agcaaccagt aaaccattga ttccaataat
    15301 gaagacactt tttacggatg ttaccggtac tgtaccggat gctgaagaga tcgcacgtaa
    15361 agccgagctt tttcgccaac aaacgggaat tgcaccgttt attgtgattt tacccgacat
    15421 taataatgaa gccagtctca gacaaaatgg taaagcgatg ctggcgcatg cgtcatcttc
    15481 attgagtaat gtaaaaggaa gtgttctgtt actatttact acccgcgaac cacggttaat
    15541 tatgatcacc aacggccagg ttgaaagtgg tctggacgat aaacatctcg gccttctgat
    15601 agaaaatcac acgctggctt atttaaatgc agatctctgg tatcagggaa tcaataatgc
    15661 attggctgtt ctacaagcac agatattaaa acaaccgacg ccgccactga cgtattatcc
    15721 gcatccagga caacagcatg aaaatgctcc ccccggcagc accaacacat taggttttat
    15781 cgcctgggct gcgacgttta tcttattttc ctggatcttt tgctacacga ctcgttttat
    15841 ttatgcgtta aaatttgcag tggcgatgac tattgccaat atgggttatc aggctttatg
    15901 tttgtatatc gataatagct ttgcgattac cagaatatcg ccactctggg cagggctaat
    15961 tggtgtctgt acgtttattg ccgcattgtt attgacgagt aaacgataaa agaggccgga
    16021 aaagtgcgcg tgtattatgc cggatggggc gcgaacgcct tatccggccc acaaaacccg
    16081 caaattcaat taattgcgtc cccttgtagg cctgataagc gtagcgcatc aggcaatgct
    16141 tcctgcttta taatcccttc agcaactttt caacaaactc aggcaccacc tggcttgccg
    16201 ggccgtaata tttctcggca aattcattac cgacctgact tggttcgaga ttcagttcca
    16261 cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc
    16321 cggaagtacc aatggcaatg aaaatatcag ccatcgacaa cgccatataa atttcatcca
    16381 tgccgagtgg catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc
    16441 aacaatggca tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt
    16501 gcgaacaacg aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc
    16561 gttcatgcag gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt
    16621 gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc
    16681 gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg
    16741 ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa
    16801 tacctgattc cgcagaaatc cctgcccctg tcagtacgag tactcttggt ttttccattg
    16861 cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc
    16921 gtttattttt acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg
    16981 ttgtttaatc ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc
    17041 gcgcgcgttc aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctatccg
    17101 ccagttgcgt tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag
    17161 ggtcaacgat ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa
    17221 cgtgcgccct tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca
    17281 acggttgatg ataatagtgt tgccagagcc acgcaaagcc ccgacctgat agataatttt
    17341 cgatacaacc aatttgacca cagccgcagc ggcgtaacgg gaaatccagc cccatcatgg
    17401 ttaacgcatc aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttaccgg
    17461 taatcggttt accgttgaaa atcagcccgc cgccaacgcc ggtgccgaga attaacccca
    17521 tcaccagtgg atattgcgta aattcatcat cccaggcttc tgaaagggca aaacagttgg
    17581 catcgttatc aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt
    17641 taccgctggc agcggggaca ttggcggcat acagcgtacc atcttctgtt tccggcattc
    17701 ccggaatacc gatgccaaca gaacctttac agccaaaacg ttggtcagct tcggctacca
    17761 gctcgcatac tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt
    17821 cccactgcaa ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat
    17881 caaacccgta atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg
    17941 actcgcgcag ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc
    18001 agcaacaatg ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg
    18061 aagtcaataa aatagatatc gctggagagg aactgatgac cgatcaactt ttcaatccgc
    18121 tcaataatcg gggtaagttg cagtgaaaca actacgccga taatcacacc acacaggcta
    18181 ccgaacagcc ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc
    18241 gcccccagcg ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag
    18301 gtggagacga tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg
    18361 gcgcggatca tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata
    18421 taaacatagc tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca
    18481 tccgtcattt taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca
    18541 tccgccagcg ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg
    18601 gcaatgtgca aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg
    18661 atcatgatcg acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg
    18721 atgataattt gctgttcgcc cgctttaaaa ttgcgccagg catccccctg aacaaacgag
    18781 ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc ccttcacctg aattgcgcgc
    18841 agattggcac cactttctac cagtccggtg aaattgatat acggcgcagc ggcggcaata
    18901 cccggcactt tttgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc
    18961 gcctcaattt caccgtgcgg taccacagca agaatgcggt tattcagttc gcgttcaaag
    19021 ccgttcatcg cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta
    19081 gaaataacag agattagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg
    19141 ccaatcaata acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg
    19201 tcagacgtcc atcacgcatt tccagttggc ggctcatacg tttcgccagt tgcaggtcgt
    19261 gagtaaccac caggaaggcg gtgccctgca agcgattcaa ttccccaagc aactggaaga
    19321 tgctgtcggc gttacgcgca tcgaggttac cggtaggttc atccgccagt accaggcgcg
    19381 ggttattcac cagcgcacgg gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag
    19441 atgggcggtg attcgcacga tgatccagcc ccaccgcttt taacatctca agtgcacggc
    19501 tgttgatttc agcgggcttt ttcttgccaa tcagcagcgg catagccaca ttttccaggg
    19561 cggtaaaatc cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca
    19621 gctctgcttt cgccgccgaa gacagtttgc tcatcggttg accgttaaag atcacatcgc
    19681 cggaggttgg cgtatccagc ccgcccaaca agtgcagcaa ggtgctttta ccggaaccag
    19741 agctaccgac gatcgccatc atttcacctt cgccgacgct gaaactgaca ttgtgcagca
    19801 catcggtttg cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga
    19861 tcttattcat aacgtaaagc ctcagcgggt tgagtggcgg cagcgcgcca tgaagggtaa
    19921 agcgtagaca gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt
    19981 tcgatagcga ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta
    20041 tttaactggc tggcaagcag ggcgccaagc gccgctccga ggatcgcacc gataatcccg
    20101 gcgctggccc cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt
    20161 tgcaggatcg ctacttcgcc ctgcttctcc attaccatca gccctagtga ggtaataatg
    20221 ttaaacgccg caacggcgac aatcaggctc agcagtaaac ccatcatatt tttttccatg
    20281 cgtacggcct ggaacaactc gcctttacga tcacgccagt cctgccattt gctgccttca
    20341 ggcagttttt gctgacttaa tgagtcgact ttcagcggct catccagcca caaacgccag
    20401 ccggtaatat tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc
    20461 atttcatagc catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc
    20521 tgacttggaa tacgccccat cggcgtgaac tggctggcag atggtaccat catgcggatt
    20581 tgatcaccgc gattaacgcc tagctgtgag gcaagttgtt cgccgaggat gacattatat
    20641 ttccccggct cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt
    20701 tgcgccgggt cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc
    20761 acatcaccgg tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc
    20821 gggagttgct gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg
    20881 ccaaggatgt tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc
    20941 agcgccatca ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag
    21001 cgatccgctg cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac
    21061 atgaaatccg tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt
    21121 aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg
    21181 gctaaattgc catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc
    21241 aatacagaca gatacccatt acacctcggg tcataacccg caggatcaac cgcatgtaaa
    21301 ttaaaaaaga gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag
    21361 ggttatgtat ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatctga
    21421 ccacttttca gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt
    21481 accttgcccc ctttcgtatg ccggtttttt tctttatttc cggctatttg attcgccgct
    21541 atatcgacag cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg
    21601 tgctggcgct ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg
    21661 cacctgagcg cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt
    21721 tcctgcacgg gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct
    21781 atttcgtggt atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac
    21841 tgttgagtgt ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc
    21901 gcaatttgcc ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta
    21961 aagaggtgcc gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg
    22021 gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga
    22081 agctgtttta tcaatacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga
    22141 ttggctccaa caccattgct atctacacca cccatcgcat tctggttgaa atattcagct
    22201 taactctgct tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc
    22261 tgctggttta tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa
    22321 aactttcaca gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg
    22381 tcagttactc ccgctaagcc atcgccccag ccggggcgat tcaatttgct aaaccatgtc
    22441 gattacggat aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat
    22501 cctaacgaga atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa
    22561 gcgggtgagc agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg
    22621 gaaattgccg aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct
    22681 ctgcgtttgc atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac
    22741 tgggaaactc ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt
    22801 tccacccttt accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg
    22861 cttatgcagc gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa
    22921 ggtcagcgcc tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat
    22981 gttgaccagg tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc
    23041 ccgatgggga gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg
    23101 cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg
    23161 cccgcgcacg aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc
    23221 gataccttcg aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca
    23281 ttacctgccg ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg
    23341 ttcagttatt tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc
    23401 gaacgtttcc aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc
    23461 ccgctgttgc caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa
    23521 aactggcccc gggtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat
    23581 ttaggtttcc agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat
    23641 gcgctgcgta agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa
    23701 ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt
    23761 atgcgtcttg atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat
    23821 ggttttgtcg ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa
    23881 cgtgttgccc gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt
    23941 aaccttgcgg agctgcatat tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc
    24001 tatgccggaa tgaccacgct cgaagcgggc ggcattactg gcgagtattt gatgctcacc
    24061 tatgccaacg acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac
    24121 gcaggtggcg cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc
    24181 gcgcggcaga aagcggcgga aagagtgcgt gatgtggcgg cggaattgct ggatatctac
    24241 gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg
    24301 ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta
    24361 cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt
    24421 ggtaaaacag aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggtg
    24481 gcggtgttgg tgcctaccac ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt
    24541 ttcgccaact ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag
    24601 acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa
    24661 ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac
    24721 cgcttcgggg tgcgtcataa agagcgtatt aaagcgatgc gcgcgaacgt ggatattctg
    24781 acgcttactg caacgccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac
    24841 ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag
    24901 tatgacagcc tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt
    24961 tattatctct acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg
    25021 gtgccagaag cgcggatcgc catcggtcac gggcagatgc gcgagcgcga actggaacgg
    25081 gtgatgaatg atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa
    25141 accgggatcg acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt
    25201 ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt cgcatcatca ggcatatgca
    25261 tggttgctga cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg
    25321 attgcctcgc tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt
    25381 cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc
    25441 tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg
    25501 tcgctggaag atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg
    25561 ccagatgatt tcattcctga cgtgaatacg cgtttgtcgt tctataaacg tattgccagc
    25621 gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg
    25681 ctgccggatc cggcgcgtac cctgctggat gttgcccgtc tgcgccagca agcgcagaaa
    25741 ctggggatca ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag
    25801 aatcacgtta atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc
    25861 cttgatggtc cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc
    25921 gaatgggtac gccagtttat gcgtgaactg gaagagaacg cgatcgctta atggtaaatc
    25981 gggggcgttt ctgcgccccc atctgcaaca tttacaaatt ctttgcactt ccctgcacta
    26041 tccgacactg tcaccatcca taattcaggc ttatctgttt attacaataa ccttatattt
    26101 attatggatt tttggtgatg atcaaaacgc gtttttctcg ctggctaacg ttttttacgt
    26161 tcgccgctgc cgtggcgctg gcgctaccgg caaaagccaa cacctggccg ctgccgccag
    26221 cgggcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc
    26281 tggaagccat cgccaaaaaa tataacgtcg gctttctcgc tctgttacag gctaaccccg
    26341 gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg caaaccctac
    26401 ttccagacgc gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt ctctattact
    26461 acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag ttaggtggtg
    26521 acacgctgac accgacaatg gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga
    26581 cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg cctgcggtag
    26641 tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg
    26701 gcgtttattt gcttcacggt acgaacgccg atttcggcat tggcatgcgg gtaagttctg
    26761 gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc accccaggca
    26821 ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc
    26881 tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag ctgctgccaa
    26941 ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac gctgaagtga
    27001 tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt catcaagtga
    27061 gcccacaaac tctgtaaatc ctggataaaa aaaatccccg cgacacggca tgtcgcgggg
    27121 aaaaaatcag cagtagcagg cattaatgag ggttaatgct tatttataaa ttactgctgt
    27181 tccatggagg gtattcggac cggttacaga agtaatacgg aaagattttg cgcccatctc
    27241 atccgctttt tgcgccagct gctcttccag cgatcccaga tttgtccccg cgttagcact
    27301 gattgtaccg actttttgtt ggccttctgg cgttgactga acttcgacag ccgcaaagct
    27361 ggcaaatgac atggagctta aaatcgccgc agcgatgagg gtttttacgt ttttcataat
    27421 agtggcctta tgcagatgaa tgacgtcgca agcaattact taacga
//
LOCUS       DAPPNJ010000045        27366 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000045 DAPPNJ010000000
VERSION     DAPPNJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.045"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            39..770
                     /gene="artJ"
                     /locus_tag="RPM73_001543"
     CDS             39..770
                     /gene="artJ"
                     /locus_tag="RPM73_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDX6519916.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(945..2072)
                     /gene="rlmC"
                     /locus_tag="RPM73_001544"
     CDS             complement(945..2072)
                     /gene="rlmC"
                     /locus_tag="RPM73_001544"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDX6519917.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2113..2601)
                     /locus_tag="RPM73_001545"
     CDS             complement(2113..2601)
                     /locus_tag="RPM73_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDX6519918.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2661..3506)
                     /gene="potI"
                     /locus_tag="RPM73_001546"
     CDS             complement(2661..3506)
                     /gene="potI"
                     /locus_tag="RPM73_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDX6519919.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3503..4456)
                     /gene="potH"
                     /locus_tag="RPM73_001547"
     CDS             complement(3503..4456)
                     /gene="potH"
                     /locus_tag="RPM73_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDX6519920.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4466..5599)
                     /gene="potG"
                     /locus_tag="RPM73_001548"
     CDS             complement(4466..5599)
                     /gene="potG"
                     /locus_tag="RPM73_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDX6519921.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5694..6806)
                     /gene="potF"
                     /locus_tag="RPM73_001549"
     CDS             complement(5694..6806)
                     /gene="potF"
                     /locus_tag="RPM73_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDX6519922.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7158..7634)
                     /locus_tag="RPM73_001550"
     CDS             complement(7158..7634)
                     /locus_tag="RPM73_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDX6519923.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7722..8624)
                     /gene="rimK"
                     /locus_tag="RPM73_001551"
     CDS             complement(7722..8624)
                     /gene="rimK"
                     /locus_tag="RPM73_001551"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDX6519924.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8685..9407)
                     /gene="nfsA"
                     /locus_tag="RPM73_001552"
     CDS             complement(8685..9407)
                     /gene="nfsA"
                     /locus_tag="RPM73_001552"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDX6519925.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISDAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9391..9678)
                     /locus_tag="RPM73_001553"
     CDS             complement(9391..9678)
                     /locus_tag="RPM73_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDX6519926.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9838..10095
                     /gene="grxA"
                     /locus_tag="RPM73_001554"
     CDS             9838..10095
                     /gene="grxA"
                     /locus_tag="RPM73_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDX6519927.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10125..10502)
                     /locus_tag="RPM73_001555"
     CDS             complement(10125..10502)
                     /locus_tag="RPM73_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDX6519928.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10772..12457
                     /locus_tag="RPM73_001556"
     CDS             10772..12457
                     /locus_tag="RPM73_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDX6519929.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12632..13168)
                     /gene="rcdA"
                     /locus_tag="RPM73_001557"
     CDS             complement(12632..13168)
                     /gene="rcdA"
                     /locus_tag="RPM73_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDX6519930.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13252..14460
                     /locus_tag="RPM73_001558"
     CDS             13252..14460
                     /locus_tag="RPM73_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6519931.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLKS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14460..15275
                     /gene="ybjI"
                     /locus_tag="RPM73_001559"
     CDS             14460..15275
                     /gene="ybjI"
                     /locus_tag="RPM73_001559"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDX6519932.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15361..15645
                     /locus_tag="RPM73_001560"
     CDS             15361..15645
                     /locus_tag="RPM73_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519933.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15686..16918)
                     /gene="mdfA"
                     /locus_tag="RPM73_001561"
     CDS             complement(15686..16918)
                     /gene="mdfA"
                     /locus_tag="RPM73_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDX6519934.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17203..17799
                     /gene="ybjG"
                     /locus_tag="RPM73_001562"
     CDS             17203..17799
                     /gene="ybjG"
                     /locus_tag="RPM73_001562"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDX6519935.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17857..18615
                     /gene="deoR"
                     /locus_tag="RPM73_001563"
     CDS             17857..18615
                     /gene="deoR"
                     /locus_tag="RPM73_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDX6519936.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18662..19864)
                     /gene="dacC"
                     /locus_tag="RPM73_001564"
     CDS             complement(18662..19864)
                     /gene="dacC"
                     /locus_tag="RPM73_001564"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDX6519937.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20111..20737
                     /gene="gstB"
                     /locus_tag="RPM73_001565"
     CDS             20111..20737
                     /gene="gstB"
                     /locus_tag="RPM73_001565"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDX6519938.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20734..21849)
                     /gene="yliI"
                     /locus_tag="RPM73_001566"
     CDS             complement(20734..21849)
                     /gene="yliI"
                     /locus_tag="RPM73_001566"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDX6519939.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21960..22343)
                     /gene="bssR"
                     /locus_tag="RPM73_001567"
     CDS             complement(21960..22343)
                     /gene="bssR"
                     /locus_tag="RPM73_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDX6519940.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22556..23881
                     /gene="rimO"
                     /locus_tag="RPM73_001568"
     CDS             22556..23881
                     /gene="rimO"
                     /locus_tag="RPM73_001568"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDX6519941.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23928..25256)
                     /locus_tag="RPM73_001569"
     CDS             complement(23928..25256)
                     /locus_tag="RPM73_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX6519942.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25264..>27366)
                     /locus_tag="RPM73_001570"
     CDS             complement(25264..>27366)
                     /locus_tag="RPM73_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDX6519943.1"
                     /translation="RAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQV
                     NGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGK
                     DHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSE
                     GNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
                     VYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKD
                     AEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDER
                     NKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRN
                     LRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIAR
                     NIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFI
                     NELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLK
                     ANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIK
                     VDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLY
                     QKPMPFSAWDKSGKLVKE"
BASE COUNT         6981 a         7067 c         6557 g         6761 t
ORIGIN      
        1 ggccaaaaat cataagattg acagacggga gttccatcat gaaaaagtta gttcttgccg
       61 ctttacttgc ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt
      121 cagccaccta tccacccttt gaatctatag gtgctaataa tgagattgtc ggctttgata
      181 tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt actaatcacg
      241 cgttcgacag cctgatcccg tcactgaaat tcagaaaata tgacgccgta atctccggta
      301 tggatatcac gcccgagcgt agcaagcagg tatcgtttac cacgccctac tatgaaaact
      361 cagccgtcgt gattgccaaa aaagatacct acaaaacgtt tgccgatctg aaaggcaaac
      421 gtattgggat ggaaaacggc accacgcacc agaaatatat tcaggatcag caccccgaag
      481 tgaaaactgt ctcctatgac agttatcaga atgcctttat cgatctgaaa aatggtcgta
      541 ttgatggggt atttggtgac acagcggtgg taaacgaatg gctgaaaacc aatccgcaac
      601 tgggcgttgc tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt ctgggcatcg
      661 ctgtacgtcc ggataacaaa gctctgctgg aaaaactgaa taacgcgctg gcagcaatta
      721 aagctgacgg tacatatcag aaaatcaatg accagtggtt tccgcagtaa ttgagactga
      781 tgacaaaagt aagattgcct gatgcgctgc gcttatccgg cccacatcaa ctctgcaatg
      841 tattgattta gcatgatttt gtaggccgga taaggcgtac acgccgcatc cggcatgaac
      901 aaagcgcacc tgatcagcaa tctgaagccg cacctgcggc ttttttattg cttcaccagc
      961 agcgtcagca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg
     1021 atgcggtaac ctggcagttc gcggatatct ttcgccatgg tttgggcgtt acagctggag
     1081 tagataataa aacgcggtgc cattgttgag agataatcac atagcggttt accaatgccg
     1141 cggcgcggcg gattaaccag caccagctcc ggcacctccc cctgagcggt ggcaaactga
     1201 gtggagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc tgactgcttt
     1261 gcacaggcaa tggcctctga tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg
     1321 cagtgtaaac caaagccacc cacaccgcag aacagatccc acatatgttt aactggcagc
     1381 tgccgtaccc agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg attagtctgg
     1441 aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc cagcgcctgt
     1501 tgttcagtaa ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata
     1561 ttgacggtaa taactttcag ttgcggtagt tgttcctgta accacggcag cgccttacgc
     1621 agttgtgcca gtttggtttc agaacgcagt acaaagcgca gcatcatgcc gccatcgctc
     1681 tggctttcag tcagcagaat gtatttcagt tcgccacgct tacgcgccac gttgtagggt
     1741 gtcagccccg cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaatgaggca
     1801 ggataaagcg ggcagtcaca aaggtcttct ggtgtgccgt cgcgatgcag cataccgagc
     1861 agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc
     1921 gggcctgaca ccggcgcgca ccattcctca accggaaaat cggcgagcag atttttaaga
     1981 tcggcggttt tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga
     2041 cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga
     2101 cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag
     2161 catcaggatg agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt
     2221 tgattcaccg ggaatgctga acagctccgg ataaccgtac cccagcgaag ccgcccacaa
     2281 gtaacttgcg gcggtgattt gtgtcagcag atatagccag cgcgcccagc gacgcccttt
     2341 caccagtgaa aacgcacacc agatctcaat gaaaaccagc accagactgc ttaaaaagac
     2401 cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc
     2461 cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc
     2521 cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa
     2581 tcccaacgtt tcgtcttcca tgaaaacaat gccgcggcat gcgcggcatt atgtagccag
     2641 gttggcaaat tttagtgtct tcagccatgt cttgcacgct ggatatcgcg tatccgctgt
     2701 ttttctgcgc gagccatcag ataccaggcg ataaatccga caattccgac cgcaccgaga
     2761 attaacgttg caagggcgtt gatttccgga ttcaccccca tccgcacgct ggaaaagacc
     2821 agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgatcac cagatcatca
     2881 agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc
     2941 gtaatgacga aaaacacttt cagcggcgtc gcaccgagat ccatcgctgc ttcttctatc
     3001 gagcgatcca gttcccgcaa tcttgaagaa ataacgaccg ccacataagc cgtacaaaaa
     3061 gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc aatggcatga
     3121 gcaagcgcga cgaataataa caacagcgac aagcccgtga tgacatctgg catcaccagc
     3181 ggcgcggtga tcataaaggc aaagccattt gatccgcgaa atctgccaaa gcgcaccagc
     3241 accaccgccg caatagtccc gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc
     3301 aggcttaaac caaccgcact catcatcgcg tcatcgcgca ataactcacc ataccagcgc
     3361 gttgaccagc cggcccacac cgtcaccagc ttcgagctgt taaacgaata gataaccagc
     3421 atcagcattg gcgcgtagag aaaggtaaag cccagcagca aaatcacaat ccgccagggc
     3481 gaacgaacta ccggtaaatt attcatccgt gttctcccac gcttttttgc tggtgtttgt
     3541 ggaaccacat tatcggcaca attagcagca gcaacatgat gatcgctacc gccgaggcca
     3601 ccggccagtc gcggttattg aaaaactctt gccatagcac gcgcccgatc atgatgctgt
     3661 ccgggccacc gagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca
     3721 tcgatccggc aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca
     3781 gcggtcgtgc accgagatcc agcgctgctt ccaccagcga ataatcaata cgaatcaacg
     3841 cggtataaat cggcagtacc ataaacggca cgtaggcgta aacaatgcca atataaactg
     3901 ccagattggt atgaagaatg gtcagcggtt gatcgataac ccccagccac agcagaaaat
     3961 tattcagcac accgttgttt tttaatattc ccatccaggc ataaacgcgg atcagaaacg
     4021 aggtccacga cggcaggatc accagtagta ataaaatatt acgggtcgaa ggcttactgt
     4081 gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc gaaatcgcag
     4141 ccacctgtag cgactggaga taagcatcga aatagagcgg atcgtcggtc agttgcagaa
     4201 aattaccgag attaagagtg atggacagtt gcccgtcagc ccactccatc agttcggtat
     4261 aaggtggaat agcgcgcgcc atctccgcca ggcttatttt aaagacaatc agaaatggca
     4321 gcagaaacag caagatcaac cagatatatg gcaacgcaat gaccagtttg cgcccatgct
     4381 tcatttgcag ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag
     4441 gttcaagtgt attcatcttt gctccttaaa ccgtcagcac cacacagctg tccacttccc
     4501 agcacaaacg cacttcgtcg ccccaggtcg gtaacccttt acgatgacga tgggcgtttt
     4561 gtagctgggc gctaatcatc tgcccacttt tcagacgaac gtgatacacc gaaagatcgc
     4621 cgagataggc aatgtgtatc acctccccca ccgcgaagtt acaaccattg gcgggcggct
     4681 cttcgcaaag catgattttt tccgggcgca gcgctacgtg taccggcacg ttatcgacca
     4741 ctgaagcatc cgcatcgact ttcagtggat gcaccagccc cggcgaatca agcaccaggc
     4801 catcttcctg acgctctttg agtacacctt caaagacatt aaccgaaccg ataaattcag
     4861 cgctgtagcg ggtagttgga tgctcgtaga tctcttccgg ctcgccaatc tggacaaatt
     4921 tcccgcgatt cataatggcg atacgccccg ccatggtcat cgcctcttcc tgatcgtggg
     4981 tgaccatcac acaagtcaca ccgacgcgct ccagaatatc caccacttca agctgcatcc
     5041 ggtcacgcag ctttttatcc agcgcgccca tcggctcatc gagcagtaat agtttcgggc
     5101 gtttggcaag gcttcgggcc agggccacac gctgtcgctg accaccggaa agctgatgcg
     5161 gtttgcgttt ggcgaactcc tgcatgtgca ccaggccgag catctcatgg acccgactgg
     5221 caatttccgc tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc tgttccacgg
     5281 tcatatgcgg aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg
     5341 gcacctgtga caaatcgacg ccatcaagca ttatctgtcc ggcagaaggt tgttcgaaac
     5401 ctgccagcat acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga
     5461 tttcaccttt gtagatggtc aggctgacat catccaccgc atgttgacca tcgtaggatt
     5521 tggtgaggtt gcggatttct aatagcggcg tcagcgcctt acgggttttc gcctgcgggc
     5581 gagggatagc gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat
     5641 ggtgcgaatt gccggcgggt gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt
     5701 ccgctcttca ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact
     5761 ttcagagtga acagcttcgc acgaacatcc gcaggcggat aaatacccgg gttctcacgg
     5821 acttccgcac tcaccagcgg cgtggctgct ttattggcgt tggcatagaa cacatggtcg
     5881 gaaatatgcg ctactacatc cgggcgcagc aggtaattca ggaactgata ggcttcgtct
     5941 ttgtttttgg catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt
     6001 ggaatcgaga acgagacatt aacgccattc ttcgcttcct tcgcgcggtt tgacgcctgc
     6061 cagacatcgc ccgcccagcc aatagcgacg cagatatcgc cgtttgccag gtcgttaatg
     6121 tattgagatg aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggtagcc
     6181 ggtccggtgt aatcatccgc tttagtgctg ttgggatctt tgccgagata attcagcacg
     6241 gtagcaaaaa cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc
     6301 agattttcag gcttgaggat caagtcccag ctatcgacgg gcgcgttttc gcccagcaca
     6361 gctttaactt tatcaacgtt atagccaatc ccggtcgtcg cccacatata gggcatagca
     6421 aatttattgt cgggatcgtg tttggcgacc aacttcagca gctccggatc gagattcttc
     6481 cactccggca atttgctttt gtccagcggc tggaaaactc ccgctgtcag ctggcgttca
     6541 agaaagctgg cagatggaac caccaagtca aagccggtgc tacccgccat taatttgcct
     6601 tccagtactt cgttagagtc gaaaacatcg tagacgactt taataccggt ttctttttca
     6661 aaattggcca ccgtgtcagg ggcgatataa tcagaccagt tataaatgtg gagtgttttt
     6721 tgttcagccg cgagcgtgcc gacagagacg gccatcagag cacccgcaac cagacccgat
     6781 agccattttt tatttaaggc ggtcatatct ctttccttct gaaagttcgt taacaaacga
     6841 acaaaagttg tcacactgaa agatatgcaa taaacgtgca tattttttta tcggcgcggt
     6901 gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc atcgcaagaa
     6961 taactttagc cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt
     7021 tatgcgtatt tgctctttgt gataaagcca aatcgacaca agagaagtga aataatcttt
     7081 aagaaaaggt taaaaacagc agataatttt gcagcgcgca gccactgcac cagcgactgc
     7141 gtgctcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca
     7201 tcatcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc
     7261 tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat
     7321 aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc
     7381 acggtcagcg aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa
     7441 ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca
     7501 tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat
     7561 tcaaaaaaac tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg
     7621 accagcgatg tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc
     7681 aaaaagcgca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg
     7741 caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata
     7801 ccggtggttt tttctattcc ttccagcccc ggcgacgcat tcacctccat caccaacggc
     7861 ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct
     7921 tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga
     7981 tgcaaattgg aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca
     8041 tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc
     8101 agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctgacgcgtc
     8161 tccgccagca cgacgccaat tccctgcgtg ccttcaacca gcttgaccac cagcggcgca
     8221 ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca
     8281 ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg
     8341 gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc
     8401 gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt
     8461 aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa
     8521 agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta
     8581 cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc
     8641 agacctggtc aaaagcccgc gtatcataca tcgacgtggc ggttttagcg cgtcgcccaa
     8701 ccctgtttgt gcaaataatc cagaataaat gggcggcttt ctttaatgat tgttcggcgg
     8761 atatgatcgc tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattccgcc
     8821 agttgctcgt catactgcgc cagtgcgcct ttatccagcg gttgatagct gttttcatgc
     8881 accaaaatgg aggccggtaa acgcggctta agatccggat tatccgcagg ccagccaagg
     8941 cacagcccaa acagcggcag aacatgctgc ggtaatttaa gcagtttcgt caccgcttca
     9001 atattattgc gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcgatt
     9061 aatgcattct gcgccatcat tgccgtatca acgacaccga gcaacagttg ttccgccagg
     9121 ccgagctgag catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag
     9181 aactccgccg cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcttcacgt
     9241 aacgctttgt cggtaatgcg aataatgcta ctgcactgca aaaaactgga actggacgtc
     9301 gcacgggcgc tgttaataat cgcctcacac tgcgcgtcgg aaatgggttc atcagtgaaa
     9361 tggcgaatgg agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct
     9421 ttctgaacgt gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttgccac
     9481 ctgaagaata accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag
     9541 aatctgcact tctggccgag aaaatggctc aggcagcgac agggagtcgc ttaccgacag
     9601 cagcgccacc gccagtagca tcattccgat aaattccagt atcaacacgc ctttaggcaa
     9661 tttaccgatc gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc
     9721 actgtattgt gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt
     9781 tcagcgaata cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg
     9841 caaaccgtta tttttggtcg ttcgggttgc ccttactgtg tgcgtgcaaa agatctggct
     9901 gagaaattga gcaatgaacg cgatgatttt cagtatcagt atgtagatat tcgtgcggaa
     9961 gggatcacta aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag
    10021 atttttgtcg atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagaa
    10081 aatctggacg cctgatcgtc tgacaagccc tcgcgttgag ggctttactg attttttctg
    10141 tgctgtggtt taaacaaact actgataaat aagaaacaca gtgcccccag cgcacaccag
    10201 aacaccgcgc ttagtaacca tgccagctct tgccagaatg agcgcgtcgg tgaaaaaaac
    10261 agccgcataa tgagcattga acagggtgcc gccagcattg cgccaaatag aggtttcagg
    10321 acttctctac gctgtgaaaa gaagctggcg actgctccag gaagaatgaa aaatagcaag
    10381 ccgatttcag gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaagg
    10441 cacaccacaa tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc
    10501 actcgttcct cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta
    10561 agataaagcc gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga
    10621 tacctaatgt gattacacta gtaaaatata ttgttacttt actatcgttt aggtgcgctg
    10681 aatgaatctg cgccctgaat tctggtaaaa aacattatcg taaattacca tttctttcaa
    10741 cagcttacta gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa
    10801 tgggaattac attctgttat tatttgtggt cctcgcgctt gggctatgtc tcggaaagtt
    10861 acgacttggt tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt
    10921 aggccaacaa catttcagca ttaacaccga tgcgcttaat cttggcttta tgctgtttat
    10981 tttctgcgtc ggggtcgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa
    11041 aaattaccta atgttagcac tggtgatggt tggcagtgcg ctggtgatcg ccttagggtt
    11101 aggtaagctg tttggctggg atattggcct gacggccggt atgttagcag gctctatgac
    11161 gtcgacaccg gttctggtcg gtgctggcga tacactgcgt cattccggca tggaaagcag
    11221 gcagctctca ctggcactgg ataatctgag cctcgggtat gccttaacct atttaatcgg
    11281 tctggtgagt ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca
    11341 gaccagcgcc cagcaaatcg cccgcgaacg tggcctggac actgatgcca accgtaaggt
    11401 ttatttaccg gtgatccgcg cctatcgcgt cggcccggag ctggtggcct ggaccgacgg
    11461 caaaaatctg cgtgaactgg gtatttatcg acaaaccggc tgctacattg aacgtattcg
    11521 acgtaacggg attctggcaa atccagacgg tgatgccgtg ctacaaatgg gcgatgaaat
    11581 agcgttggta ggctatcccg acgcccatgc ccgactcgat cccagcttcc gtaacggtaa
    11641 agaagttttc gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa
    11701 aaaccataac gctgtaggta aacgtctcgc acaactgaag ttgaccgatc acggttgctt
    11761 ccttaaccgc gtcattcgta gccagattga gatgccgata gatgacaacg tcgtgcttaa
    11821 caaaggtgac gttttacaag tcagcggtga tgcccgtcgc gtaaaaacca tcgccgatcg
    11881 catcggcttt atctcgattc acagccaggt cactgacctg ctggcatttt gcgccttctt
    11941 tgttattggg ctgatgatcg ggatgatcac cttccagttc agcacattca gtttcggcat
    12001 ggggaacgct gccgggttgt tattcgccgg aattatgctg ggctttatgc gtgctaacca
    12061 cccgaccttc ggttacattc cgcaaggtgc attaagcatg gtgaaagagt tcggcttgat
    12121 ggtgtttatg gcaggcgttg gtctgagcgc cggtagcggt attaataacg gcctgggcgc
    12181 gattggcggt cagatgttga ttgccggatt aattgtcagt cttgtgcccg tggttatctg
    12241 tttcttgttc ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg gcgcaatgat
    12301 gggcgcacgc acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaacat
    12361 ccctgcgctg ggctatgcgg gcacctacgc aatcgccaac gtcctgctga cgctggcagg
    12421 gacaatcatc gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga
    12481 aatttttttg cacaaccgca gaacttttcc gcagggcatc agtcttaatt aatgccactg
    12541 cttttctttg atgtccccat tttgtggagc ccatcaaccc cgccatttcg gttcaaggtt
    12601 gatgggtttt ttgttatcta aaatttatct actaccctgc aaccctctca accatcctca
    12661 aaatctcctc gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc atcccctcaa
    12721 taaacgcatc aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc gtttgctgac
    12781 tgcgctgcat ccagttttgc attaccgttt ttaatagcgg ttttcggcta gccagcgcgt
    12841 agagctggta catcagctcc atgttatccg gcgttgcaac ctgtgagctg tagatcatat
    12901 cggtgatagc ctggcatgcg ccttgagcat cactaacatc gctaaaaaat gcctgatatt
    12961 gccgggacat aatctcagta aaactgctga acgcctccag caacaactca tcaattcctg
    13021 aaaaatagta ggtcatcgat cccaacggta ccccggcaag ggtagcaatt ttgcggtgcg
    13081 taacagcatg tattccgtaa agtttcaccg cctccagcgt ggcctggata attttttctc
    13141 gccgttgcgg atcgttagcg cgacgcatgt catttcctct gtctgcaatt gtgtacaaat
    13201 gtacacaacc ttgctaatgt tgtcttccct cttcttattt tgacgtgatc tatgaccgta
    13261 aattcttcac gtaatgcatt gaaacgccga acctgggcgc tgtttatgtt cttctttttg
    13321 ccaggcctgt taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga tattctctct
    13381 gtctcgatcg ctgaaatggg cggtgttctc tttggtctgt cgatcggttc gatgagcggt
    13441 attctctgct cggcgtggtt agtgaaacgc tttgggacac gtaatgtcat cctggtcacg
    13501 atgtcctgcg cattgatcgg gatgatgata ttaagtctgg cactctggct gaaatcgccc
    13561 ctgctctttg ccgttggtct cggcgtcttt ggggcaagtt ttggttctgc ggaagtggcg
    13621 ataaacgttg aaggtgccgc cgttgagcga gaaatgaata aaacggtttt gccgatgatg
    13681 cacggttttt atagcctggg cacgctggca ggcgctggtg tcgggatggc actgacggcc
    13741 tttggcgttc cggcaacggt gcacatttca ttggcggcgc tggtaggtat cgcgcctatt
    13801 tatatcgcca ttcaggcaat ccctgacggt acgggcaaaa atgctgccga tggcacccag
    13861 catggcgaaa aaggcattcc tttttatcgc gatatccagt tgctgttgat tggtgttgta
    13921 gtgctggcga tggcctttgc cgaaggttct gccaacgact ggttaccctt attaatggtt
    13981 gacggtcacg gttttagccc tacttccggc tcgctgattt atgccggttt taccctgggg
    14041 atgaccgttg gacgcttcac cggcggttgg ttcatcgacc gttacagtcg cgttgccgtg
    14101 gttcgggcca gtgcactaat gggggcgttg ggtattgggc tgattatttt tgtcgatagc
    14161 gcctgggtcg caggagtgtc tgttgtactt tggggactgg gtgcctcgct gggcttcccg
    14221 ctgaccattt ctgccgccag cgataccggc cccgatgcac caacccgcgt cagtgtggta
    14281 gcaacgaccg gttatctggc tttcctcgtc gggccgccgc tgctgggcta tctcggcgaa
    14341 cattatggat tacgtagtgc aatgctggtt gtactggcgc tggttattct cgcggctatt
    14401 gtcgcgaaag ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga gaatagttga
    14461 tgagcattaa attaattgcg gtagacatgg atggtacttt cttaagcgat caaaaaacct
    14521 ataaccgtga gcggtttatg gctcagtatc agcaaatgaa agcacaaggc attcgctttg
    14581 tggtcgccag cgggaatcaa tattatcagt tgatctcttt cttccctgaa attgcgaatg
    14641 aaattgcctt tgtggctgaa aacggcggct gggtagtgag tgaaggcaaa gatgttttta
    14701 atggcgagct gtcgaaggat gcctttacta ctgtcgtgga acatttgctg acgcgcccgg
    14761 aagtggaaat tattgcctgc ggaaaaaata gcgcctatac gctcaaaaaa tatgacgatg
    14821 ccatgaaaac ggtggcggaa atgtattatc accgtctgga atacgtcgat aactttgaca
    14881 acttagaaga tattttcttt aagtttggcc tgaatctttc cgatgaactg attccacaag
    14941 tacaaaaagc attacatgag gccatcggcg atattatggt gccagtccac accggcaacg
    15001 gcagcatcga tctgattatc cccggcgtac ataaagccaa tggccttcgc caactgcaga
    15061 aattatgggg aatagacgac agcgaagtgg tggtctttgg cgatggcggt aacgatattg
    15121 agatgctgcg tcaggcaggc tttagttttg caatggaaaa tgccggtagc gcggtcgtcg
    15181 cagcggcaaa ataccgcgca ggctccaata accgtgaagg cgtactggat gtgatcgata
    15241 aagttcttaa acacgaagcg ccatttgacc aataaaaacg gcagcacgcc cggcaacaca
    15301 atgcctgaat gaaggctgca taattcgcgg aaattaaccc gctgcattat ttcaggcgaa
    15361 atgattatga aaaattgtct actgttgggc gcgcttttaa tgggctttac tggtgtggcg
    15421 atggcgcaaa gtgtcaccgt cgatgtgccg agtggataca aagtggttgt ggttcctgat
    15481 tcagtcagcg ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt ctatgtggct
    15541 cctgcgccag ctccggctta ccgcccgcat ccttatgttc gccatcttgc cagcgtaggt
    15601 gaagggatgg ttatcgaaca ccagattgac gaccatcacc attaattcga gaatgcctga
    15661 tcgcacaaag caatcaggca tttttttacc cttcgtgaga atttcccatc tgtttatctt
    15721 ttaaaaagat aaccatcagc gacagccaca aaattccgtt gacaaggttg aagagattaa
    15781 acagtccgtt gcccccgttc agccaggcat gtttgctgat ttcaataccg acggtaaaga
    15841 tcagcatttg cagcattccc atcgccgcag aaaccgtacc tttactcata tcgctggcaa
    15901 acagggttaa tcgcaccagt cccgcattcg ccagaccaat gccgaaagca taaatactta
    15961 acccggcggt catccataaa tacgcgtgcg atgagataac cgttgccgca gcagcgacca
    16021 acagaccaat cataatcggc cagccgccca taattatcag cgaacgtacg gtgcggcgcg
    16081 aggtcaaacg cgctaacaac aagttacccg caattaacgc cccgaaaata ggcacttgca
    16141 gcaagccata ttcatagctg ctcaactgct cgccggtaat gatgataatc ggcgactggg
    16201 cgatccacgc cagcaatggc aggctaacga atcccagcgc cagcgccccc gccacaaagc
    16261 ggccgttctt cagcaccagc ttatagtcac gaccgagttc tttcagtgac agtttctcgc
    16321 ctatacgcgt ggcggtttca ggcatggctc gttgcagacc gaaaaaggag atcgctgcca
    16381 atgcggcaaa caaaacaaac atcccctccc agggcagcac atggatccac gccgcgccca
    16441 ccagcggacc aagtagcgga gcaatcagcg ccacgttcgc catcagcgcg gtgatcttga
    16501 tacaaaccgc ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac
    16561 agaggcttat gccctgcaag aagcgcaaca gggtgaattg ttcaatgttt tgcgccagta
    16621 atattgccag acaggtgatg ataaaccaca ccacacccgc cagcatcacc ggccggcgac
    16681 caatacgatc cgacagcggc cccaggagcc attgtaaaaa catcccgccc gccagatacg
    16741 cggtcatcga agtaggaacc caatcaatgc ccgcctgata ttgttccacc acggccaaca
    16801 taccgggttg aatcatatcg ttgccgatat aggttgaaaa ttcgtaaagc accagacaga
    16861 gagggaaaag taacgcctga cgtccaagcc tggcaccgga agctaattta ttttgcatgc
    16921 aatttcttcg ccaataataa tcgcgcaaag tttaataaaa gcgcagctaa cgagaaagcg
    16981 aattttgtag ctgaaaccac ggttaagcac attcttacat tattgcgagt atagctacgc
    17041 tttctttaag ttttatttaa cctatgcccg ttacaatcac ccaccgtaaa caggccgctt
    17101 gagggaaata agacgatgcc gctttaccca gtttaacctg cactttattc tcaacgactt
    17161 gcctgtattg gctccctttt aatcactttg cgtcgggaag ttatgctgga aaatttgaat
    17221 ctctctctat tctctcttat taacgcgacg ccagactcgg ctccgtggat gatctcgttg
    17281 gcgattttta ttgctaaaga tttgattacc gtggtaccgc tgctggccgc ggtgctttgg
    17341 ttgtgggggc ttacagcaca acggcaactg gtgataaaaa tcgctatcgc gctggcggtc
    17401 agcctgtttg tgtcctggac gatgggacat ctttttccgc acgaccgacc ctttgtcgaa
    17461 aatatcggct ataacttcct gcatcatgcg gcggatgact cattcccaag cgatcacggt
    17521 acggtgattt tcacctttgc actggcattt ttatgctggc atcgcctgtg gtccggctca
    17581 cttttaatgg tgctggccgt cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg
    17641 ccgctggata tgctcggtgg attgctggca ggtatgattg gctgccttag tgcccagatt
    17701 atctggcaag cgatggggca taaactctat caacgtctgc aatcgtggta tcgcttctgt
    17761 tttgcattac cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa aagtgtagta
    17821 ttgagcggct cgcttcaata actattcaga gggattatgg aaacacgtcg cgaagagcgt
    17881 atcgggcagc tgctgcaaga attaaaacgc agcgataagt tacatcttaa agacgccgcc
    17941 gccctgcttg gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc
    18001 gtcgttttgc tcggcggcta tattgttctg gaaccgcgca gtgccagcca ttacctgtta
    18061 agcgatcaaa aatcccgcct ggtggaagaa aaacgccggg cggcaaaact ggctgcgaca
    18121 ctggtagaac ccgatcagac cctctttttt gactgtggca ccaccacgcc gtggattatt
    18181 gaagcgattg ataatgaaat cccttttacc gccgtttgtt attcgctaaa tacctttctg
    18241 gcgctgaaag agaaacccca ttgccgcgca tttctgtgcg gtggtgaatt tcacgccagc
    18301 aacgccattt tcaaacccat cgattttcag caaacgctga ataatttttg cccggatatc
    18361 gctttttatt ctgcggcggg cgtgcatgtc agtaaaggcg ctacctgttt taatcttgaa
    18421 gagttgccgg taaaacactg ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac
    18481 cacagtaaat ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt
    18541 gtggtgagtg attgttgccc ggaagatgag tatgtgaagt acgcgcagac gcaccgtatt
    18601 aagttgatgt attaatgacg tataaccgga tgacgtttcg cgccatccgg ttatcagaag
    18661 attaagagaa ccagctgccg aaccactgat ggaatttcat catcacgaaa tcccacatcc
    18721 gaccaaagaa tccgccctct tccacatttt ccatcacgat cagcggacgc tgctcaatgg
    18781 atttaccgtt aagctggaaa tcaatggtcc cgacaacctg accttttttc agcggtgcgg
    18841 taagctgcgg ttccgttaac gtgtagctcg ccttcaggtt tttcagctgc ccacgcggta
    18901 tggtcacgga gcccgcttcg cctgccccga gattcacttc gctcttatca ccaaaccaga
    18961 cgcgctgagt cacaaaggtg gcatcaggtt taattggcgt cacagtttca aagaagcgga
    19021 aaccccaggt caataatttc tcagactcat taaaacggat acggtcagtt ttcgccccca
    19081 gcactacgga gattaaacgc atatcaccct gggtagccga agcaaccaga ttatatcccg
    19141 cacctgccgt ggttcctgtc ttcatgccat caacattcag attgctgctc cacagcagac
    19201 ggttacggtt aggctgacga attttgttga aggtgaactc tttctcttta tgaatggcgt
    19261 actcttccgg cacatcgtgg atcaatgctt tacccagcaa tgccatatcg cgcgcggtac
    19321 tgaactgccc cggcgcatcc agaccgtgca ccgtctggaa ggtagtgttg gtcagaccca
    19381 gttttttggc ataaccattc atcaaaccaa taaatgactc ctggctcccg gcaacgtaat
    19441 cggccagcgc aatacaggcg tcattaccgg actggataat cacacctttg ttcaagtctg
    19501 ccaccgaaac ctgatcgccc ggtttgagga acattaccga tgaaccacgc agtgccggat
    19561 ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc ggtgagttta atcttatcgg
    19621 ccttaagcgc ctgcccaacc acatagctgg tcatgatttt agtcaggctc gcgggatcca
    19681 gtttctcatc cgcgttgcct tctgccagca ctttaccgct ggcgtaatcc attaaaatcc
    19741 atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc cgccgcgaat gccgttgggg
    19801 caaaaaggaa taaaaatgca gaacccgctg caagaccacg aaggagagag gagtattgcg
    19861 tcataaatgc cacccgagta tccattcaaa aaaaatacgt tatatcgccg tatgataagt
    19921 tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa
    19981 gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct
    20041 taaaagttaa ctttacttca ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa
    20101 aggggtgagt atgattacgc tgtggggtcg gaataattca accaacgtaa aaaaagtatt
    20161 gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg
    20221 gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg
    20281 tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc
    20341 acagtacggg caaaaacgtc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa
    20401 atggatggac tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt
    20461 agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg
    20521 cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga
    20581 cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg
    20641 cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc
    20701 cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa
    20761 ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc
    20821 acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt
    20881 gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt tttgctgcca
    20941 ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca
    21001 gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa
    21061 gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg
    21121 gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca
    21181 cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcac
    21241 accggattcc tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac
    21301 cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag
    21361 agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt
    21421 agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc
    21481 acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata
    21541 acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc
    21601 gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc
    21661 tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg
    21721 taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt
    21781 tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg
    21841 tcgatgcata acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac
    21901 aaattcattt ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt
    21961 caggccttct caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc
    22021 agacaatttt caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca
    22081 ctcatcaaac atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac
    22141 tcttcctgat ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt
    22201 ttaaaaaagt tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc
    22261 ctcagttgca cgtatgcggc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg
    22321 attcgctgtc tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa
    22381 atagtatggc tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa
    22441 caaaatttac atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca
    22501 gaactctgta gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag
    22561 caaagtaact ccccagccga aaatcggctt tgtttccctt ggctgtccaa aaaaccttgt
    22621 tgattcagag cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta
    22681 tgacgatgcg gacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga
    22741 atcactggaa gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg
    22801 tctgggggca aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg
    22861 gcctcatagc tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca
    22921 caacccattc ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc
    22981 ctatctgaaa atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat
    23041 gcgcggcgac ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt
    23101 agatgcgggc gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga
    23161 tgttaaacat cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct
    23221 gtgcgaacag ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc
    23281 gcatgtggac gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat
    23341 tccgttgcag cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga
    23401 tcgccaactg gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc
    23461 aacctttatt gtcggcttcc ctggcgagac agaagaagat ttccagatgc tactcgactt
    23521 cctgaaagaa gcgcgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc
    23581 agacgccaat gccctgcctg accaggttcc ggaagaagtt aaagaagaac gctggaaccg
    23641 tttcatgcag ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga
    23701 aattctggtg attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga
    23761 tgcaccggaa atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga
    23821 tatcctgcgt gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta
    23881 attaattcag gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta
    23941 atctgagccc tgccgttttt cttactctta tacagccgct catccgccag cattttcaag
    24001 cgttcgacgg tggtacattc atttgaatgc gctacaccga tactggtact taaattaatg
    24061 cttcccacct ggaaatgaac gatttctttt agaatcgata gcaactcttc cagcgttcca
    24121 cccgtataaa tcaccgcaaa ttcgtcaccg ccgaagcggt agattttacc tttatctacc
    24181 gggatctgtg atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga
    24241 ccgaaggtgt cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa ataaccgcct
    24301 ttttccggca gcgcttttaa atcttcgtca aaggattttc tgcccattgc tttggtcagg
    24361 tcgtccagga acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag
    24421 aaaagcccgc catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca
    24481 ataccacgat taatcactct gcgtaccaga tagaggcaac tggtacccga tatcaccgcc
    24541 accaatgcgg caaagaaaat aatcaatgta cagacccgca aataaacatc ttttttatac
    24601 gcaaatacgt acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg
    24661 aagatggcgt ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga
    24721 gagaacatca ggttctcgct gtccatcgcc agcgattttg acagtaaaga aaaacgctcc
    24781 agaccaagca aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag
    24841 gttttaccgt caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc
    24901 ataatatctg cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa
    24961 atcagataag gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg
    25021 cgcaaaccaa ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc
    25081 acgctacgcc gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt
    25141 ttcaggctat acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa
    25201 atcatgataa aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt
    25261 aaactactct tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg
    25321 gtacagatac ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc
    25381 gctctctacg ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat
    25441 gcgcacaatt tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat
    25501 gctgtctgga ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc
    25561 aatcgatatc cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt
    25621 aattcgctcg atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt
    25681 ttctttcatg atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga
    25741 aacattaata tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa
    25801 ttcacccatt gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc
    25861 agcaatggta atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca
    25921 caacgcctcc gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag
    25981 gaatagttcg ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc
    26041 tatttcgttg agtttcgcgt catcacaaaa aataaactcg gagaacatat ggtcacggat
    26101 actggaaatc gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg
    26161 ttcacaaatg gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc
    26221 gtttaccatc gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata
    26281 gagatcggca ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg
    26341 acacaatttc ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacggcttc
    26401 cagaccacac agtttgatga tccccacagc cagatactga ttattgttgg actcaataat
    26461 cgccatattt ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt
    26521 tgaccgttca atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc
    26581 tttcgcatcg ccttcgcgaa ttttcagagc ctcgatttga tccgttgagt cgggcaaaaa
    26641 cgccagcgta ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt
    26701 catgaaatat accaccagca acacgataat aatgaaagca aacggtagat aaaaaagttt
    26761 atctgacgcg ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt
    26821 atttggcact gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata
    26881 attaccttcg ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat
    26941 ctcatgacct tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc
    27001 gttcagatac cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc
    27061 tttgccgttg atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt
    27121 gtatgccgga ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga
    27181 tgggttaaaa tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg
    27241 ttcctgatga taaacaacga ttttatcatt cgccctccgt gaaaagagat aatatttatt
    27301 atcatcaagt accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac
    27361 ggcgcg
//
LOCUS       DAPPNJ010000046        26701 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000046 DAPPNJ010000000
VERSION     DAPPNJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.046"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            325..1419
                     /gene="gcvT"
                     /locus_tag="RPM73_001571"
     CDS             325..1419
                     /gene="gcvT"
                     /locus_tag="RPM73_001571"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDX6519944.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1443..1832
                     /gene="gcvH"
                     /locus_tag="RPM73_001572"
     CDS             1443..1832
                     /gene="gcvH"
                     /locus_tag="RPM73_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDX6519945.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1951..4824
                     /gene="gcvP"
                     /locus_tag="RPM73_001573"
     CDS             1951..4824
                     /gene="gcvP"
                     /locus_tag="RPM73_001573"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDX6519946.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGASATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5090..5833
                     /locus_tag="RPM73_001574"
     CDS             5090..5833
                     /locus_tag="RPM73_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6519947.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5890..7323)
                     /gene="bglA"
                     /locus_tag="RPM73_001575"
     CDS             complement(5890..7323)
                     /gene="bglA"
                     /locus_tag="RPM73_001575"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDX6519948.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7368..7679
                     /locus_tag="RPM73_001576"
     CDS             7368..7679
                     /locus_tag="RPM73_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDX6519949.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7843..8502
                     /locus_tag="RPM73_001577"
     CDS             7843..8502
                     /locus_tag="RPM73_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDX6519950.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8698..9678)
                     /gene="ygfZ"
                     /locus_tag="RPM73_001578"
     CDS             complement(8698..9678)
                     /gene="ygfZ"
                     /locus_tag="RPM73_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDX6519951.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9921..10187
                     /gene="sdhE"
                     /locus_tag="RPM73_001579"
     CDS             9921..10187
                     /gene="sdhE"
                     /locus_tag="RPM73_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDX6519952.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10168..10575
                     /locus_tag="RPM73_001580"
     CDS             10168..10575
                     /locus_tag="RPM73_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDX6519953.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10615..11136)
                     /gene="fldB"
                     /locus_tag="RPM73_001581"
     CDS             complement(10615..11136)
                     /gene="fldB"
                     /locus_tag="RPM73_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDX6519954.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11248..12144
                     /gene="xerD"
                     /locus_tag="RPM73_001582"
     CDS             11248..12144
                     /gene="xerD"
                     /locus_tag="RPM73_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDX6519955.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12169..12879
                     /gene="dsbC"
                     /locus_tag="RPM73_001583"
     CDS             12169..12879
                     /gene="dsbC"
                     /locus_tag="RPM73_001583"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDX6519956.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12885..14618
                     /gene="recJ"
                     /locus_tag="RPM73_001584"
     CDS             12885..14618
                     /gene="recJ"
                     /locus_tag="RPM73_001584"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDX6519957.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14709..15807
                     /gene="prfB"
                     /locus_tag="RPM73_001585"
     CDS             join(14709..14783,14785..15807)
                     /gene="prfB"
                     /locus_tag="RPM73_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDX6519958.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15817..17334
                     /gene="lysS"
                     /locus_tag="RPM73_001586"
     CDS             15817..17334
                     /gene="lysS"
                     /locus_tag="RPM73_001586"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDX6519959.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17377..17925)
                     /gene="idi"
                     /locus_tag="RPM73_001587"
     CDS             complement(17377..17925)
                     /gene="idi"
                     /locus_tag="RPM73_001587"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDX6519960.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17980..18051
                     /locus_tag="RPM73_001588"
     CDS             17980..18051
                     /locus_tag="RPM73_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519961.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(18048..18173)
                     /locus_tag="RPM73_001589"
     CDS             complement(18048..18173)
                     /locus_tag="RPM73_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519962.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18175..19623)
                     /gene="uacT"
                     /locus_tag="RPM73_001590"
     CDS             complement(18175..19623)
                     /gene="uacT"
                     /locus_tag="RPM73_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDX6519963.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            20059..21978
                     /gene="ygfT"
                     /locus_tag="RPM73_001591"
     CDS             20059..21978
                     /gene="ygfT"
                     /locus_tag="RPM73_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDX6519964.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     RVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21978..22466
                     /locus_tag="RPM73_001592"
     CDS             21978..22466
                     /locus_tag="RPM73_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDX6519965.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22502..23869)
                     /gene="ghxQ"
                     /locus_tag="RPM73_001593"
                     /gene_synonym="ygfQ"
     CDS             complement(22502..23869)
                     /gene="ghxQ"
                     /locus_tag="RPM73_001593"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDX6519966.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23905..25221)
                     /gene="guaD"
                     /locus_tag="RPM73_001594"
     CDS             complement(23905..25221)
                     /gene="guaD"
                     /locus_tag="RPM73_001594"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDX6519967.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     TMAKRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25239..26639)
                     /gene="xanQ"
                     /locus_tag="RPM73_001595"
     CDS             complement(25239..26639)
                     /gene="xanQ"
                     /locus_tag="RPM73_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDX6519968.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
BASE COUNT         6667 a         6698 c         7079 g         6257 t
ORIGIN      
        1 atgtcattat tttttgattt agtgtttttt gacatttttt tagctcttaa tattgcctta
       61 ttcaaattag ctttctaatg gcatcatctt tatatagaaa ctggtgtgtt ttttggtttt
      121 ttattctgtc gcgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc
      181 ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg
      241 accgggagtg gctccgatgc tgggtttcgt ggtgataatt tcaccatgaa aagttgtcag
      301 ccccgcttat tcaatgagga caagatggca caacagactc ctttgtacga acaacacacg
      361 ctttgcggcg ctcgcatggt ggatttccac ggctggatga tgccgctgca ttacggttcg
      421 caaatcgacg aacatcatgc ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg
      481 accatcgtcg atctccgcgg cagccgcacc cgggagtttc tgcgttatct gctggcgaac
      541 gatgtggcga agctcaccaa aagcggcaaa gccctttact cggggatgtt gaatgcctct
      601 ggcggtgtga tagatgacct catcgtctac tactttactg aagatttctt ccgcctcgtt
      661 gttaactccg ccacccgcga aaaagacctc tcctggatta cccaacacgc tgaacctttc
      721 ggcatcgaaa ttaccgttcg tgatgacctt tccatgattg ccgtgcaagg gccgaatgcg
      781 caggcaaaag ctgccacact gtttaatgac gcccagcgtc aggcggtgga agggatgaaa
      841 ccgttctttg gcgtgcaggc gggcgatctg tttattgcca ccactggtta taccggtgaa
      901 gcgggctatg aaattgcgct gcccaatgaa aaagcggccg atttctggcg tgcgctggtg
      961 gaagcgggtg ttaagccatg tggcttgggc gcgcgtgaca cgctgcgtct ggaagcgggc
     1021 atgaatcttt atggtcagga gatggacgaa actatttctc ctttagccgc caacatgggc
     1081 tggactatcg cctgggaacc ggcagatcgt gactttatcg gtcgtgaagc cctggaagtg
     1141 cagcgtgagc atggtacaga aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg
     1201 cgtaatgaac tgccggtacg ctttaccgat gcgcagggca accagcatga aggcattatc
     1261 accagcggta ctttctcccc gacgctgggt tacagcattg cgctggcgcg cgtgccggaa
     1321 ggtattggcg aaacggcgat tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgaca
     1381 aaacctgttt ttgtgcgtaa cggcaaagcc gtcgcgtgat ttactttttt ggagattgat
     1441 tgatgagcaa cgtaccagca gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag
     1501 aagccgacgg cacttacacc gttggtatta ccgaacatgc tcaggagctg ttaggcgata
     1561 tggtgtttgt tgacctgccg gaagtgggcg caacggttag cgcgggcgat gactgcgcgg
     1621 ttgccgaatc agtaaaagcg gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg
     1681 cggtaaacga cgcactgagc gattccccgg aactggtgaa cagcgaaccg tatgcaggtg
     1741 gctggatctt taaaatcaaa gccagcgatg aaagcgaact ggaatcactg ctggatgcga
     1801 ccgcatacga agcattgtta gaagacgagt aacggcttta ttcctcttct gcgggagagg
     1861 atcagggtga ggaaaattta tgcctcaccc tcactctctt cgtaaggaga gaggttcaca
     1921 attcactgca cgtttcagga accatcgctc atgacacaga cgttaagcca gcttgaaaac
     1981 agcggcgctt ttattgaacg ccatatcgga ccggacgccg cgcaacagca agaaatgctg
     2041 aatgccgttg gtgcacaatc gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt
     2101 cagcttgcga ccccgccgca ggttggcgca tcggcgaccg aatacgccgc actggcagaa
     2161 ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt acatcggcat gggttacacc
     2221 gccgtgcagc taccgccggt tatcctgcgt aacatgctgg aaaatccggg ctggtatacc
     2281 gcgtacactc cgtatcaacc tgaagtctcc cagggccgcc ttgaagcact gctcaacttc
     2341 cagcaggtaa cgctggattt gactggactg gatatggcct ctgcttctct tctggacgag
     2401 gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc
     2461 aaccgcttct tcgtggcttc cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt
     2521 gccgaaacct ttggttttga agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag
     2581 gacgtcttcg gcgtgctgtt acagcaggtt ggcacaaccg gtgaaatcca cgactacacc
     2641 gcgcttatta gcgaactgaa atcacgcaaa attgtggtca gcgttgccgc cgatattatg
     2701 gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg atattgtttt tggttcggcg
     2761 caacgcttcg gcgtgccgat gggctacggt ggcccacacg cggcattctt tgcggcgaaa
     2821 gatgaataca aacgctcaat gccgggccgt attatcggtg tatcgaaaga tgcagctggc
     2881 aataccgcgc tgcgcatggc gatgcagact cgcgagcaac atatccgccg tgagaaagcg
     2941 aactccaaca tttgtacttc ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt
     3001 tatcacggcc cgattggcct gaaacgtatc gctaaccgca ttcaccgttt gaccgatatc
     3061 ctggcggcag gcctgcaaca aaaaggtctg aagctgcgcc atgcgcacta tttcgacacc
     3121 ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc gtgccgaagc ggctgaaatc
     3181 aacctgcgta gcgatattct gaacgcggtt gggatcaccc ttgatgaaac aaccacgcgt
     3241 gaaaacgtaa tgcagctttt caacgtgctg ctgggcgata accacggctt ggacatcgac
     3301 acgctggaca aagacgtggc tcacgacagc cgctctatcc agcctgcgat gctgcgcgac
     3361 gacgaaatcc tcacccatcc ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc
     3421 tatatgcact cgctggagcg taaagatctg gcgctgaatc aggcgatgat cccgctgggt
     3481 tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc caatcacctg gccggaattt
     3541 gccgaactgc acccgttttg tccgccggaa caggctgaag gttatcagca gatgattgcg
     3601 cagctggctg actggctggt gaaactgacc ggttacgacg ccgtttgtat gcagccgaac
     3661 tccggcgcac agggcgaata cgcgggcctg ctggcgattc gtcattatca tgaaagccgc
     3721 aacgaagggc atcgcgatat ctgcctgatc ccggcttctg cgcacggaac taaccccgct
     3781 tctgcacata tggcaggaat gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc
     3841 gatctgactg atctgcgcgc gaaagcggaa caggcgggcg ataacctctc ctgtatcatg
     3901 gtgacttatc cttctaccca cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc
     3961 gtgcatcagt tcggcggtca ggtttacctt gatggcgcga acatgaacgc ccaggttggc
     4021 atcacctcgc cgggctttat tggcgcggac gtttcgcacc tcaacctgca taaaactttc
     4081 tgcattccgc acggcggtgg tggtccgggt atgggaccga tcggtgtgaa agcgcatctg
     4141 gcaccgtttg taccgggtca tagcgtggtg caaatcgaag gcatgttaac ccgtcagggt
     4201 gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc caatcagctg gatgtacatc
     4261 cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg tggcaatcct caacgccaac
     4321 tatattgcca gccgcctgca ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc
     4381 gtggcgcacg aatgtattct cgatattcgc ccgctgaaag aagaaaccgg catcagcgag
     4441 ctggatattg ccaagcgcct gatcgactac ggtttccacg cgccgaccat gtcgttcccg
     4501 gtggcgggta cgctgatggt tgaaccgact gaatctgaaa gcaaagtgga actggatcgc
     4561 tttatcgacg cgatgctggc tatccgcgca gaaatcgacc aggttaaagc cggtgtctgg
     4621 ccgctggaag ataacccgct ggtgaacgcg ccgcacattc agaacgaact ggtcgccgag
     4681 tgggcgcatc cgtacagccg tgaagttgca gtattcccgg caggtgtggc agacaaatac
     4741 tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc
     4801 gtaccgatta gcgaatacca gtaattcact gattcgacta tcttctaaag gcgcttcggc
     4861 gcctttttag tcagatgaca aagtacaaaa gcgctcagac agtcccctca cccctttggg
     4921 gagagggtta gggtgagggg aacaggccgg cactggcgcg aatatttacc ctcaccccgg
     4981 ccctctccct gaaagggcga ggggaaaagc gtgccaacat tgaagattga gccagtttgt
     5041 tagcaatctc aaagatacgt caacgaatta atttttctcg gaaaaacaaa tggctatagc
     5101 acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact gcattactgt tggcgcaaga
     5161 agggtatacg gtggcggtta attatcagca aaacctccac gcggcgcagg aagtgatgaa
     5221 cttaataacg caagccggtg gcaaagcatt cgtgctccag gcggatatca gcgacgaaaa
     5281 ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat gaaccgctag cagcgctggt
     5341 caataacgcc gggatcttgt ttacccagtg caccgttgaa aaccttaccg cagagcgaat
     5401 caaccgagta ctttccacca acgtgacggg atattttctc tgctgccgcg aggcggtaaa
     5461 acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc
     5521 ctcacggttg ggttcgccag gggaatatgt tgattacgcg gcatcgaaag gggcgattga
     5581 tacgttaacc accggactat cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt
     5641 gcggccaggg tttatttata ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga
     5701 tcgcgttaag tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc
     5761 cattgtctgg ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc
     5821 gggcgggaaa taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac
     5881 ttcatcgact taaagcttct cgccgttgct ggcaatcacc tcttggtacc agttaaagct
     5941 cttcttacgt gaacgcgaca tatcgccagc accgtcgtca tgtttattca catagataaa
     6001 gccgtagcgt ttgctgtact gcccggtggt gaacgatacg cagtcgatgc agccccacgg
     6061 tgtgtagccc atcagatcca cgccatcgta agtcaccgct tttttcatct cttcgatatg
     6121 ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc
     6181 gtaagcgcca aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc
     6241 gcaaagggca tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac
     6301 atacgggttc ggtacgctgc cttcgaaacc agagatcgca tcgccggtgc cgccttcggc
     6361 cttcactgcg ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcacgcag
     6421 tacatcgaga tcgccgtctt ccattttgat gttaaatccg cgacgctccc actcgttcaa
     6481 cacataggac gggtaatagc cgcgtagctg cacatcggta aagacgtagc gttcgcgcat
     6541 cgactcctga gcgaacatca catcgtccgg gttacagcag tagggataga gcggcaccat
     6601 cgccagcata cagccgactt tcatctccgg gttaatgcga cgcgcagctt tcaccgccag
     6661 ggcgctggcg acaaactggt gatgcagcac ctggtacatc gtctcttccg ggttgtcatg
     6721 ctcggtataa accacgccgg agcagcagta accgaacagc ggtgcacgcc agttacgctg
     6781 gttgttaatt tcgttgaagg tcatccagta tttgacttta tgcttatagc gttcaaacac
     6841 cacttccgcg aaacggacaa agaaatcaac cactttacgg ttggtccagc taccgtattg
     6901 ctgcaccaga tgcagcggca tttcaaagtg ggagagggtg atcaccggtt cgatgttgta
     6961 tttcagcagt tcatcgaaca tatcatcgta gaacttcagc ccttcttcgt ttggctgagc
     7021 ttcatcacct tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc
     7081 catttcggca aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg
     7141 gtttggatag tatttgcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc
     7201 gccaccggtc agaacgtcac aaatgctcgg ccctttgccg cctttgttcc agccgccttc
     7261 gacctgatga gcggcaactg cgccgcccca taagaaatct ttcggtaagg tgagtttttt
     7321 cactatcata ggctccagat gagtcaattc ttacaggaaa tagtaccatg cagccaaacg
     7381 acatcacttt ttttcaacgt ttccaggatg acattctggc tgggcgtaaa accatcacca
     7441 tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt gcttcgtgtc gggcgttttg
     7501 aagatgacgg ttatttttgc acgattgaag tcaccgcaac ctcaaccgta acgctggata
     7561 cgctgacgga aaaacatgca gagcaggaaa atatgaccct gactgaactg ataaaggtca
     7621 ttgccgacat ctatcccggt cagacacagt tttatgtgat tgaatttaaa tgtctttaaa
     7681 ttgaactcac gcctgttagt tgaaatttcg catttaattt catgattttt aggagattag
     7741 tctcaaatca gaatttattt tagctaacag gtgttcactg gaactattct cagttacgct
     7801 ggagaggtat acatgtgcgt gtacgtttct ccggagtaag ttatggttca gaagcccctc
     7861 attaagcagg gatattcgct ggcagaggaa attgccaaca gcgtcagtca cggcattggg
     7921 ttggtgtttg gtatcgttgg gctggtgttg ctactggttc aggcggtgga tctcaatgcc
     7981 agcgccacag cgataaccag ttacagcctc tatggcggca gtatgatcct gctgttcctc
     8041 gcttcgacgc tctatcacgc cattcctcat caacgggcaa aaatgtggct gaagaaattt
     8101 gaccattgcg ctatttacct gttgattgcc ggaacctaca cgccgttttt gctggtgggg
     8161 ctggattctc cgttagcgcg cgggttgatg attgttatct ggagcctggc attgctgggt
     8221 attctgttta aactgaccat cgcgcaccga ttcaaaattt tatctctggt gacctatctg
     8281 gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc
     8341 agcgttacct tactggcggt aggcggcgtg gtttattcgc tcggggtgat tttctacgtc
     8401 tgcaaacgca ttccatacaa ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg
     8461 tgccactttc tggcgatcta tttgtatatt gggcaggcgt aatgggtatg cctttgtagg
     8521 cctgataaga cgcgccagcg tcgcatcagg catcggagcg caaccgccgg atgcggcgtg
     8581 aacgccttat ccgatctacg ggcctcgtcc ctgttgtgat aaaggcatca aatcccagac
     8641 atgataagac gcgtcagcgt cgcatcaggc gataaatgcc tgatgcgata cagagattta
     8701 ctcttcgagc gaatacggca gcggctcgat atgcagtgta ttcgcatcgt cgcgtacgcg
     8761 gaagatgctg tccggttcca tatcgttatt catgacgacc tgtaccacga cctgaccatc
     8821 ttccagtttt accgcagcca gcaccgtacc ggtacgacgc cagttctcgc ccattttcag
     8881 ctctaagtct tcaccagctt ccggcagtcg gctggcgcta cctgccagca accagagcgc
     8941 acgtttattg gcaccacgga attttgctcg cgccaccatc tcttgtccgg tataacagcc
     9001 tttcttaaag ctgataccgc ccagcgcctg gagattggtc gcctgtggga taaactgccc
     9061 gctgttggcg gcatcaatca ccgggaaacc cgcttcaatg tttaatgcca gccactgttg
     9121 gctattgttc agttccgctt caccgcgcag tttatcggtc agcatattag cagtagcttc
     9181 atcggttacg atcaggaaac gttctgccgg gtgttcaaac catagcaaag tggtcgcgcc
     9241 ttctttgact acctgttttt ctttcgaagg cagttcgcta aagagatttg ccagcgcggc
     9301 gcgcgcctga aaaccggcaa caccaagcag cacacgctcg tcgtctggcg cgatggtcac
     9361 tttagagaat accgcatatt ttttcagttc agtcagctgc ggttcacgca cgctgcgccg
     9421 ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt aaattgctcc acattttacc
     9481 tttggcgtcg caatgggcgg cgagcaggtg ctgatcttct gccatctggc tgacatctgc
     9541 tgtcacctga ccctgcatat atttttcgct gtccgcgcca gtaatggtgg caagcgccca
     9601 gtcatcaagc gtcatcagcg tcagcggtaa acgggcagaa gccgtaggct gacggggagg
     9661 aaaaggtgta aaagccataa tagagtcctg agttgcttaa cggcgtatta atgactaatg
     9721 gtaaaagagc tatgaggcaa tgcaagtgct ttcactacgc gatctgtgcc ttctgcgagg
     9781 cgacttccaa aaaaatgaag ccattgaatt gttgtgaatt aatttcgcga tcgcgatggt
     9841 gtttactgat attcccgtaa aagaaggcga aaaacacgtt acaatagccg ggtactggat
     9901 tttcgtgaga caggaagaag atggacatta acaacaaagc ccgcattcat tgggcatgcc
     9961 gccgtggtat gcgcgaactc gatatttcaa tcatgccgtt tttcgaacat gagtacgaca
    10021 gcttaagcga tgacgaaaaa cgcatcttta ttcgtctgct ggaatgtgac gatccggacc
    10081 tgtttaactg gctgatgaat cacggtaaac cggccgatgc agaactggaa atgatggtcc
    10141 gactcatcca gacacggaac cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc
    10201 tggcgcgcac agtggctttc cttgctgatt catgggctgg ttgccgctgt tattttactc
    10261 atgccctggc cactcagtta caccccgtta tggatggtgt tactttcgct ggtggtgttt
    10321 gattgcgttc gcagccagcg gcgtattaat gctcgccagg gggaaattcg cttgttgatg
    10381 gacgggcgtt tgcgttggca agggcaggag tggtgcatcg tcaaagcacc gtggatgatt
    10441 aagagcggca tgatgctgcg tttacgttct gatggcggta aacggcaaca tttatggctg
    10501 gcagccgaca gcatggacga agccgaatgg cgggatttac ggcggatttt gttgcaacaa
    10561 gagacgcaaa gataaagatg acggcaaagc atatgcgcta agccgtcagt gagatcaggc
    10621 gtaatgctct gccatttcgt tgaggatttg ctcgcaccag ctctgaatac gctcgtcgct
    10681 aaggtcatac tggttagttt catccagcgc cagacccacg aacagttgcc cgtcagcaat
    10741 caccggtttc gggctggtaa attcatatcc ttccgttggc cagtagccga cgaacttcac
    10801 gcctttggtc gagagtttgt catgcagcat accgagcgca tcgaggaacc actcgccgta
    10861 tcccagttga tcgccaagcc catacagcgc aacaatttta ccttcaaggt tcaggtcgtc
    10921 gagctgatcc cagacggctt cccagtcttc ctggatttca ccaaaatccc aggtcgggat
    10981 acccagaatg agcacatcgt actgctccat taatttcggg gagtcgtcct tgaggttatg
    11041 taaggtcacc agttctgggc cgataatatc gcggattttt tctgccgcca tttcggtgta
    11101 acaggtgctg gaaccgtaaa aaagacccat attcataacg taagcttctc gattctgctg
    11161 tggcttttgc agctagtgta tcagaatcgc tttagtcacg gcacaataag gcataatgga
    11221 tcaaatgagt gaccataagg ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt
    11281 cttgatgctc tgtggctgga aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc
    11341 gatctgtcaa tgatggtgga gtggttgcat caccgcgggt tgacgctggc gacggcgcaa
    11401 agtgacgatt tgcaggcatt actggcagaa cggctggagg gcgggtataa agccaccagc
    11461 tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt atctttatcg cgaaaagttt
    11521 cgtgaagacg atcccagtgc gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa
    11581 gatttaagcg aagcgcaggt cgaacgttta ttacaggcac cattaattga tcagccactg
    11641 gagctacgcg ataaagccat gcttgaagtg ttgtatgcta ccgggctgcg tgtctctgaa
    11701 ctggtcggac tgacaatgag tgatatcagc ctgcgtcagg gcgtagtgcg ggtcattggt
    11761 aaaggcaaca aagagcgcct ggtaccgtta ggtgaagagg cggtttactg gctggaaacc
    11821 tatctggaac atgggcgtcc gtggctgctg aatggtgtgt caattgacgt gttgtttccc
    11881 agccagcgtg cgcagcagat gacgcgacag accttctggc accgcattaa acattatgct
    11941 gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc
    12001 actcatttat taaatcatgg tgcggattta cgcgtggtgc agatgctact gggccacagc
    12061 gatctttcca ccacgcaaat ttatacgcat gtcgctaccg agcgtctgcg gcaacttcat
    12121 caacagcatc acccgcgggc gtgatgtctg aaaagaacgg gaagatttat gaagaaaggt
    12181 tttatgttgt ttactttgtt agcggcgttt tcaggttttg ctcaggctga tgacgcggca
    12241 attcaacaaa cgttagccaa aatgggcatc aaaagcagcg atattcagcc cgcgcccgta
    12301 gctggcatga agacagttct gactaacagc ggcgtgttgt acatcaccga tgatggtaaa
    12361 catatcattc aggggccaat gtatgacgtt agcggcacgg ctccggtcaa tgtcaccaat
    12421 aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg
    12481 caggaaaaac acgtcatcac cgtgtttact gatattacct gtggttactg ccacaaactg
    12541 catgagcaaa tggcagacta taacgcgctg gggatcaccg tgcgttatct tgctttcccg
    12601 cgccaggggc tggacagcga tgcagagaaa gaaatgaaag ctatctggtg tgcgaaagat
    12661 aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg tcgcaccagc cagctgcgac
    12721 gtggatattg ccgaccatta cgcacttggc gtccagcttg gcgttagcgg tactccggca
    12781 gttgtgctga gcaatggcac acttgttccg ggttaccagc cgccgaaaga gatgaaagaa
    12841 ttccttgacg aacaccaaaa aatgaccagc ggtaaataat tcgcgtgaaa caacagatac
    12901 aacttcgtcg ccgtgaagtc gatgaaacgg cagacttgcc cgctgaattg cctcccttgc
    12961 tgcgccgttt atacgccagc cggggcgtgc gcagtgcgca agaactggaa cgcagtgtta
    13021 aaggtatgtt gccctggcag caactgagcg gcgtcgaaaa ggccgttgag atcctttaca
    13081 acgcttttcg cgaaggaacg cggattattg tggtcggcga ttttgacgcc gacggcgcga
    13141 ccagcacggc tctaagcgtg ctggcgatgc gctcgcttgg ttgcagcaat atcgactatc
    13201 tggtaccaaa ccgtttcgaa gacggttacg gcttaagccc ggaagtagtc gatcaggccc
    13261 atgcccgtgg cgcgcagtta attgtcacgg tggataacgg tatttcctcc catgcgggcg
    13321 ttgaacacgc tcgctcgttg ggcattccgg ttattgttac cgatcaccat ttgccgggcg
    13381 aaacattacc cgcagcggaa gcgatcatta accctaactt gcgcgactgt aatttcccgt
    13441 cgaaatcact ggcaggcgtg ggtgtggcgt tttatctgat gctggcgctg cgcacctttt
    13501 tgcgcgatca gggctggttt gatgagcgtg gcatcgcaat tcctaacctg gcagaactgc
    13561 tggatctggt cgcgctggga acagtggcgg acgtcgtgcc gctggacgct aataatcgca
    13621 ttctgacctg gcaggggatg agtcgcatcc gtgccggaaa gtgccgtcca gggattaaag
    13681 cgctgctgga agtggcaaac cgtgatgcac aaaaactcgc cgccagcgat ttaggttttg
    13741 cgctggggcc acgtctcaat gctgccggac gactggacga tatgtccgtc ggtgtggcgc
    13801 tcttgctgtg cgacaacatc ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa
    13861 accagacgcg aaaagagatc gaacaaggaa tgcaagttga agccctgacc ctgtgcgaga
    13921 aactggagcg aagtcgcgac acgctacccg gcgggctggc aatgtatcac cccgaatggc
    13981 atcagggcgt tgtcggtatt ctggcttcgc gcatcaaaga gcgttttcac cgtccggtta
    14041 tcgcctttgc gccagcaggt gatggtacgc tgaaaggttc aggtcgctcc attcaggggc
    14101 tgcatatgcg tgatgcactg gagcgattag acacactcta ccctggcatg atactgaagt
    14161 ttggcggtca tgcgatggcg gcgggtttgt cgctggaaga ggataaattc gaactctttc
    14221 aacaacggtt tggcgaactg gttacagagt ggctggaccc ttcgctattg caaggcgaag
    14281 tggtgtcaga cggtccgtta agcccggccg aaatgaccat ggaagtggcg cagttgctgc
    14341 gcgatgctgg cccgtggggg cagatgttcc cggagccgct gtttgacggt catttccgtc
    14401 tgctgcaaca gcggctggtg ggcgaacgtc atttgaaggt gatggtcgaa ccggtcggcg
    14461 gcggtccact gctggatggt attgctttta atgtcgatac cgccctctgg ccggataacg
    14521 gcgtgcgtga agtgcaactg gcttataagc tcgatatcaa cgagtttcgc ggcaaccgca
    14581 gcctgcaaat tatcatcgac aatatctggc caatttagcg tcatcttctc tataaaaaag
    14641 agcgtggatt gggtacaatc ccgctcttat caccgcattt tgactagctc aataaaagaa
    14701 atcagaccat gtttgaaatt aatccggtaa ataatcgcat tcaggacctc acggaacgct
    14761 ccgacgttct tagggggtat ctttgactac gacgccaaga aagagcgtct ggaagaagta
    14821 aacgccgagc tggaacagcc ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt
    14881 aaagagcgtt cctccctcga agccgttgtc gacaccctcg accaaatgaa acaggggctg
    14941 gaagatgttt ctggtctgct ggaactggct gtagaagctg acgacgaaga aacctttaac
    15001 gaagccgttg ctgaactcga cgccctggaa gaaaaactgg cgcagcttga gttccgccgt
    15061 atgttctctg gcgaatatga cagcgccgac tgctacctcg atattcaggc ggggtctggc
    15121 ggtacggaag cacaggactg ggcgagcatg cttgagcgta tgtatctgcg ctgggctgaa
    15181 tcgcgtggtt tcaaaactga aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa
    15241 tccgtgacga tcaaaatctc cggcgattac gcttacggct ggctgcgtac agaaactggc
    15301 gttcaccgcc tggtgcgtaa aagcccgttt gactccggcg gtcgtcgcca cacatcgttc
    15361 agctctgcgt ttgtttaccc ggaagttgat gatgatattg atatcgaaat caacccggcg
    15421 gatctgcgca ttgacgttta tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc
    15481 gaatctgcgg tgcgtattac ccacatccca accgggatcg tgacccaatg ccagaacgac
    15541 cgttcccagc acaagaacaa agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa
    15601 ctggagatgc agaagaaaaa tgccgagaaa caggcgatgg aagataacaa atccgacatc
    15661 ggctggggca gccagattcg ttcttatgtc cttgatgact cccgcattaa agatctgcgt
    15721 accggggtag aaacccgcaa cacgcaggcc gtgctggacg gcagcctgga tcaatttatc
    15781 gaagcaagtt tgaaagcagg gttatgagga accaacatgt ctgaacaaca cgcacagggc
    15841 gctgacgcgg tagtcgatct taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac
    15901 ctgcgcgagc aggggattgc cttcccgaac gatttccgtc gcgatcatac ctctgaccaa
    15961 ttgcacgcag aattcgacgg taaagagaac gaagaactgg aagcgctgaa catcgaagtc
    16021 gccgttgctg gccgcatgat gacccgtcgt attatgggta aagcgtcttt cgttaccctg
    16081 caggacgttg gcggtcgcat tcagctgtac gttgcccgtg acgatctgcc ggaaggcgtt
    16141 tacaacgagc agttcaaaaa atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg
    16201 ttcaaaacca aaaccggcga actgtctatc cactgtaccg agctgcgtct gctgaccaaa
    16261 gcactgcgtc cgctgccgga taaattccac ggcttgcagg atcaggaagc gcgctatcgt
    16321 cagcgttatt tggatctcat ctctaacgat gaatcccgta acacctttaa agtacgctcg
    16381 cagatcctct ctggtattcg ccagttcatg gtgaaccgcg gctttatgga agttgaaacg
    16441 ccgatgatgc aggtgatccc tggcggtgcc gctgcgcgtc cgtttatcac ccaccataac
    16501 gcgctggatc tcgacatgta cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg
    16561 gtcggtggct tcgagcgtgt attcgaaatc aaccgtaact tccgtaacga aggtatctcc
    16621 gtacgtcata acccagagtt caccatgatg gaactctaca tggcttacgc agattacaaa
    16681 gatctgatcg agctgaccga atcgctgttc cgtactctgg cacaggatat tctcggtaag
    16741 acggaagtga cctacggcga cgtgacgctg gacttcggta aaccgttcga aaaactgacc
    16801 atgcgtgaag cgatcaagaa atatcgcccg gaaaccgaca tggcggatct ggataacttc
    16861 gactctgcga aagcgattgc tgaatctatc ggtatccacg ttgagaagag ctggggtctg
    16921 ggccgtatcg ttaccgagat cttcgaagaa gtggcagaag cacatctgat tcagccgacc
    16981 ttcattactg aatatccggc agaagtttct ccgctggcgc gtcgtaacga cattaacccg
    17041 gaaatcacag accgctttga gttcttcatc ggtggtcgtg aaatcggtaa cggctttagc
    17101 gagctgaatg acgcggaaga tcaggcgcaa cgcttcctgg atcaggttgc cgcgaaagac
    17161 gcaggtgacg acgaagcgat gttctacgat gaagattacg ttaccgcact ggaacatggc
    17221 ttaccgccga cagcaggtct ggggattggc atcgaccgta tggtaatgct gttcaccaac
    17281 agccatacca tccgcgacgt tattctgttc ccggcgatgc gtccggtaaa ataagcatta
    17341 cgttatgctc ataaccccgg caaatgtcgg ggttttttaa ttgtgctgcg cgaaagcaga
    17401 caacaatttt cttgcttcac tattggctgc ctgcatcacc atccacggac tgaacgccca
    17461 cggtgtggca tcaataccgt gtaatacatc tgctaaatca caccattgat aatccatcac
    17521 ttcatcatcg ttgatctgta acgcactggt ggtgcgtgcg gcaaataccg gacacacttc
    17581 attttccaca atgccattcg gatcggtggc gcgatagcga aagtcaggat agatagattc
    17641 aggaggcgta atttccacgc caagctcata acggcaacgg cggatcaccg cgtcttcgtt
    17701 gctttctccc agttgtgggt gcccacaaac cgagttagtc cacacgccag gccacgcttt
    17761 tttgctcagt gcgcggcggg taactaataa ttgtcctttg gcattaaaca gccaactgga
    17821 gaacgcgaga tgtaagcggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt
    17881 gggaactccc tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta
    17941 attctgatca ctcatcatta tagataattg aagagtgaaa tgattaacca agtgagcgtt
    18001 tatcgacaac cgcccgtttt gagcggatgc cgacaggtaa aaaccattta aaaaagtagc
    18061 atcatatatt tgaagcgact ttcaatggcc attgcaacac agtcagaaat gacaggaata
    18121 gagagagtaa aaagcaacag cagactgaat atagcgcgca ttaaaagatt catattattc
    18181 tccatgctca tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt
    18241 taaatcttta tcagagacgg attccttcac caggtcagca tgatgctgat agccattaaa
    18301 gaagacgttc agcacaacgg cgctaagggt tgcgagcata atgccgctat gtagcagcgg
    18361 ttgcagtacg gccggtaact tagaaaagaa atcgtgagag agcgtcggag tcatgccaac
    18421 gccgagactg atcgccacaa tatagaggtt gtaacggttg gtggtgtagt tacagcgcga
    18481 cagaattcga atccctgtcg ccagtaccat gccgaacatc accagcccag cgccgcccag
    18541 cacaaattgc ggaatggagg cgaccagcac cgccattttt ggcaccatgc caaacaggat
    18601 taatataatt cccgaagaaa tacacaccca gcggctatga acgcgcgtca cgctaaccag
    18661 gccaacgttt tgtgaaaatg acgtgtgggg gaagctgtta aacgttccgc ctatcattgt
    18721 ccctacgcca tcgacacgca gcccgcgaat aatatcgtgt gaagagagtt tacgaccaac
    18781 tatttcaccc agtgccagga acatccccat cgactcgata aacacgatga ttaacacggc
    18841 agtcatggtc agaatggaaa cgggatcaaa aatcggcata ccaaacgaca tcggggtgac
    18901 aatcgcaaac cacgaggcat catgtagccc ggataaattg acttcattca tcatccacga
    18961 aagtaaaaag ccaaatacaa tccccagtaa tacggcgacg ttggacataa atcctttcgc
    19021 atagcgagta atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg
    19081 attaccatat tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat
    19141 gctaagcccg atagaagtaa taaccacacc ggtaaccagt ggcgggaata aaggcatcaa
    19201 gcgaccgata agtggcgcta ataatgtggt gataaaaccc gcggcgatag tggcaccaaa
    19261 tatccccagc aggccgatat ccgggttcat cccaatggct atcattggtg ttacagcagc
    19321 aaaggtcacc gacataatca ccggcaggcg gatccccata aagcggccga taccgataca
    19381 ttgcaataat gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc
    19441 ttcttttgag aggcccagtc ggtcaccaat cataagagga acagcgactg cacctgcgta
    19501 catgacaagg acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc
    19561 aacctcatca gttgggcggt cttgccctga agatgaggga agttgggaat ctatggcgct
    19621 catcagtgtt tctcctttcg cccgccagag taaaaagcaa ccgtttacgt taatgaaact
    19681 gtctaaacac atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa
    19741 ttgcggaggc aatcacataa tcgtggttaa accacgaaaa tgagaagaaa acctgacaat
    19801 ggaattatct gtaattttct catattgata tttcattaat cattctggca tgtcgcactc
    19861 tcgtatttaa tcgtttttat ctggatagtg cccttttggt cggcaatgtt gaaataatga
    19921 aagcggcaca gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg
    19981 tcatgctacc taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat
    20041 taaatgaaag ggatgcaaat gaataagttt atcgctgctg aagctgcgga atgtataggc
    20101 tgtcatgctt gtgaaattgc ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt
    20161 cacagtgact ttcgaccgcg tatccacgtt gtagggaaag gccaggctgc gaatccggtg
    20221 gcctgccatc actgcaacaa tgccccttgc gttacggctt gtccggttaa tgctctgact
    20281 ttccagtccg atagcgtaca actggacgag caaaaatgta ttggttgtaa aagatgcgca
    20341 atcgcttgcc cctttggcgt cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt
    20401 tgtaaccagc gcagttccgg cacgcaagcc tgtattgaag tttgcccaac acaggcgtta
    20461 cgactgatgg acgataaagg gttacagcag ataaaggtgg cccgccagcg caaaacggca
    20521 gcaggaaaag cgtcatcagt cgctcagcca tctcgcagtg cagcgttgct ccccgttaac
    20581 tcgcgtaaag gcgcagataa aatttcagca agtgaacgga aaaaccactt tggcgaaatc
    20641 tattgcgggc tggatccact gcaagcgact tatgagagtg accgctgtgt ttattgtgcc
    20701 gaaaaagcta actgcaactg gcattgcccg ctgcataacg ctattccgga ttacatccgt
    20761 ctggtacagg aaggaaagat tattgaagcg gcagaacttt gccaccagac cagttcctta
    20821 ccagaaatct gcggcagggt atgcccacag gaccgtcttt gtgaaggcgc atgtactttg
    20881 aaagatcact ctggcgcagt ctctatcggt aatctggaac gctacatcac cgataccgcg
    20941 ctggcgatgg gctggcgtcc cgatgtcagc aaagttgttc cccgtatcga aaaagtggcg
    21001 gtgattggcg ctgggccagc agggttaggg tgtgctgata ttctggcgcg cgcaggagtt
    21061 cgggttgatg tctttgatcg ccatccagaa attggcggta tgctgacttt tggcattcct
    21121 cctttcaaac tcgataaaac ggtattaagc cagcggcgag agatattcac cgcaatggga
    21181 atcgactttc atctcaactg tgaaattggc cgcgatatta cctttagcga tttaacttct
    21241 gaatatgatg cagttttcat cggcgtgggg acttacggga tgatgcgagc agatctgccg
    21301 catgaagatg cgcccggtgt cattcaggct ctaccgttcc tgactgccca tacccgccag
    21361 ctcatgggat tgccggagtc tgaagagtat ccgctgacgg acgtggaagg taagcgagtc
    21421 gtggtattgg gcggtggcga tacgacaatg gattgtttgc ggacttccat tcgcctcaat
    21481 gctgccagcg tgacctgcgc gtatcgtcgt gatgaagtca gtatgccggg ctcgcgcaaa
    21541 gaggtggtta atgcgcgcga ggaaggtgtc gagtttcagt tcaatgttca accgcaatat
    21601 atcgcttgtg acgaagatgg gcgtttaact gcggtgggcc tgattcgtac cgccatgggt
    21661 gagccggggc cggatggtcg ccgtcgtcct cgtccggtag cgggttcaga gtttgaattg
    21721 cccgccgatg ttctgattat ggcctttggt ttccaggcgc atgccatgcc gtggttgcag
    21781 ggcagcggaa ttaaactcga taaatggggc ctgattcaaa ccggtgacgt cgggtattta
    21841 cctacccaga cgcatctgaa aaaagtcttt gctggtggtg atgcagttca tggcgcggat
    21901 ctggttgtca ctgcaatggc cgcaggaagg caggcggcgc gcgatatgtt aactctgttt
    21961 gatacgaagg catcgtgatg aaatcgttaa ttatcgttaa tccggctgac tgtattggct
    22021 gccgtacttg tgaagtagcc tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg
    22081 atgtcttttt accccggctg aaggtacagc gactggacag catcagtgcg ccggtgatgt
    22141 gccatcagtg tgaaaacgcc ccttgtgttg gcgcttgccc cgtgggggcg ctgacgatgg
    22201 gcgagcaggt ggtgcagacc aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg
    22261 cgtgcccgtt tggaatgatc accattcagt cgttgccggg ggatacccga caacaaattg
    22321 tgaaatgcga tctctgtgaa cagcgggaag aggggccagc ctgtgttgaa tcttgcccaa
    22381 cacaggcgct gcagttgctt actgaaagag aactcaggcg agtccgccag cagcgtattg
    22441 ttgccagtgg tgagaatcct ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct
    22501 attagattgc ccaaccaccc gcgtaaaatg cgaccagtgc ggcagtaatg atcaccgtac
    22561 caatattcaa cttttgccat tcgcgagcaa agacacggcc tacgaccagt gtcacaaagc
    22621 ccagcataat accggtaacg atattacaag ttagaacgat gaacacggca cataccagac
    22681 cagccattgc gtcaataaaa tcgttgaaat ccagcttcga gacgttgctt aacatcagca
    22741 aacccacgta catcagagcg ggtgcagtgg cgtaaccagg gatcaaaaat gacagcggtg
    22801 ataagaacag aatcagcagg aataacgccc ccactacggt tgcggtcaac cctgttttac
    22861 ccccggcggc ggttcctgcc gctgattcga tataaaccgc tgcgggcgct gcgcccacca
    22921 gaccggagaa tattgaactt actgagtcac tggtcagggc tttgccgcca ttgatgatct
    22981 ggttgtcttt atccaacaaa ttcgcctgac cggcgacggc acggatggtg ccagtggcgt
    23041 cgaacactgc ggtcatcacc aatgccagta cactcggaag tacggttggc tggagtgcgc
    23101 ccataatatc gaggctgaaa atcagagact taccatcttc gccagtcaga cttggcatcg
    23161 ccaccagacc gtggtatttc accgctgggt caaagattaa gccgataatc gaaattgcga
    23221 taatcaccaa caagatcccg ccgggtacgc ggcacttctc cagaccgaag atgaccgcca
    23281 gccccagcaa gctcatcatc accgggaagg aggtaaacgc accgagcgcc actggcaagc
    23341 cttcaatcgg gtttttgata accataccca caccgttagc agcaatcagc agcagaaaca
    23401 ggccgatacc gatacctgta ccgtgagcga tacccatcgg taaattacgt aagatccagg
    23461 tacgcacacc ggttacggaa atagcggtga agatgacgcc catcagaaag accgcgccca
    23521 gtgcgacagg aacgctaatt tgttgcccga gtaccagact gaatgcggta aatgccgtca
    23581 aggaaatcgc acaaccaatc gccattggca aattggccca caaccccatc agcaacgagc
    23641 cgaagcccgc gaccagacag gtggcaacaa acacagctgc gggaggaaaa cctgctttgc
    23701 ccagcattcc cggaacgacg ataacggaat aaaccatggc cagaaaggtc gttaagccag
    23761 ccagtacttc ctgacgaacg gtactgccac gagcggtaat tttaaaataa ttatccagcg
    23821 cgccctgtgg ctttggtgcg tccggtgttt gtaggatgtc tccagacata atgatgtcct
    23881 cacgaggatg ttaaagtttt attattagtt gcgttcgtac accagacgac catcaacgta
    23941 ggtgcggtag atcgaacggt catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa
    24001 agaaacagag ttgtcatagc gcagctgctg tagcggagtg gcggtgggtt ccatcaccac
    24061 gaaatcagcc tctttgccag gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt
    24121 cgctccgccg agcgtggcca ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa
    24181 ttgcaatact ttgtaggctt cgttcagcgt ttgcagcatg ttgaaagtgg ttccggcacc
    24241 gatatccgtt cccatgccca ctttaacttt cttctgccat gcttttttca agttgaataa
    24301 gccgctgccg aggtaaaggt tggaggtcgg acagaaagca atgctggatt tggtttcgct
    24361 gagacgatcc cactcttttt cttcgagatg gacgcagtga gcaaagacac agtttttacc
    24421 ggtcaggccg tactgatggt aaacatccag ataaccatca tggtcaggat aaagcgattt
    24481 cacccaggca atttcatctt tgttttcaca gagatgggta tgtacccacg tatccggata
    24541 ttcttctttc aggcgtttcg ccatcgtcat ctgttcagga gatgaggtag gggcgaagcg
    24601 tggcgtaatc gcatacaaca gacgaccatt tttgtgccag cgctcgatca gttctttgct
    24661 ttggtgatag ctgctttcgg cagtgtcgag cagataatcc ggtgcgttgc ggtccatcat
    24721 caccttaccg gcaatcatac gcatattgat atggctggcg gcttcaaaca gcgcatcaac
    24781 ggattgcgga tgaacagtgc caaacaccag cgccgtggtg gttccgttac gtaaaagctg
    24841 cttgatgaag aacgccgaca tttcgcgggc gtactctaaa tcctcataac gacgttcagt
    24901 agggaaggtg tgtttattca accactccag caattgttca ccataggccc ccaccatttc
    24961 actttgcgga taatggatat gtgtatcgac aaagcccggt actatcagtt tgccgcgata
    25021 gtcgcgcacg cgaatggtgt caggaatttg atgctttccg ttttcccatt cgccaaacca
    25081 ttccactttt ccctgtttaa tgagtaataa accatcctca ataaaccgca gcgcagaggc
    25141 aatctcctct gggttatcga ctgtacgggt gacatcaata aaactgcctc gtaccgcttt
    25201 taacgtgtgt tctcctgaca tcattgactc ctttactgtt aatccatctc ttccgctgag
    25261 gtaataccag gcagaacgtt ttcctgtcgg tagccaccag ggaggataat atttaataaa
    25321 atcgcagtta acccgccagc acaaataggg ttttcaacta gtacataaat agaggctggc
    25381 aatattttaa aaatttcggg atcgtaggag acgccaagtc ctaaacctaa agaagtggcg
    25441 acaataagtg tttctcggcg ctttaaaccg ttggtgatga ttatgcgaat cccagcgatg
    25501 gcaatcatgg aaaacatcaa cgtcattgcg cctcccagaa ctgccgaggg aatggtcgtg
    25561 aagaagccgc caatcatcgg aaacaaaccg aggataacca gcattaccgc gatggttcgc
    25621 ccgacataac gtgaagcgac gccagtcatc tgaataaccc cattattttg cgcaaacgtg
    25681 gttaagggta atgacccgac agcggaggcg ataacagaaa ccagaccgtc tgccagcacg
    25741 ccgcctttca gtcgggactg atactcttct ccctgaatgg gtcggcggga aaccattgcc
    25801 gtggcggtga tatcgccgac agcttccaac acgctaagca gataaatcgt gccaaccacc
    25861 aggaactgat ggaagctaaa actaaagccg tatttgaacg gatgtgggat ggtgattaac
    25921 ggcaaattgc gcatactgct gaaatccacc atgcccaggc ataacgatgc gatatagccg
    25981 acgcatagcc caatagcgat ccctcccatg cgtagcaacg gactgcgaca gcagttaaag
    26041 ccgatcacca caatcaaaac caataaacca acgccgagat gttcgtaatt gccgaaggta
    26101 ccgctgcttt tggcagcaaa tcctccacca aaatcgataa tgccgacttt aatcaggctt
    26161 aagccgatca tcagtaccac aataccgctg acagtaggcg taataacccg acgtaaatag
    26221 ggcaagataa atgaagatcc gacaaccaga aatgcgccaa cgaaggagac gccgaggagc
    26281 gacgacatga ttaactcttc gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc
    26341 atgaccgtaa caaatgaaaa attgactgac tgaattgaga gtaggccaga accgacgatg
    26401 ccatagcggt ttacttgtaa ccaggtacca ataccagagg cgatcatcgc cattgaaaca
    26461 agataggcag ttgtttcagc ggaaagctgt aaggccgccc ccacgattaa cgcgggggtt
    26521 accatcggaa cgaaaattgc caacagatgg gtaatggcac caacgagagc ctgatgaaag
    26581 ggagggcgat cctccagttc aaatataagg tcagaacctg catggtttat atcagacatc
    26641 ccttcactcc ttttaaagta aatttgatca tctgaattag taaaataaat tcccacgaat
    26701 t
//
LOCUS       DAPPNJ010000047        26272 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000047 DAPPNJ010000000
VERSION     DAPPNJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.047"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..374
                     /locus_tag="RPM73_001596"
     CDS             <1..374
                     /locus_tag="RPM73_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDX6519969.1"
                     /translation="LESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRK
                     NQIRTFWRKAGVITRQLDGHGFTMQDWRDYLSYVGENCRWMFEERPNHQRGTVWHKKG
                     FDFLLNDNTYLKVREGEHDDR"
     gene            364..1800
                     /locus_tag="RPM73_001597"
     CDS             364..1800
                     /locus_tag="RPM73_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDX6519970.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YVRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            1876..2202
                     /locus_tag="RPM73_001598"
     CDS             1876..2202
                     /locus_tag="RPM73_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519971.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATRDEFNEAWARL
                     SERGYL"
     gene            2199..2399
                     /locus_tag="RPM73_001599"
     CDS             2199..2399
                     /locus_tag="RPM73_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519972.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            2411..2662
                     /locus_tag="RPM73_001600"
     CDS             2411..2662
                     /locus_tag="RPM73_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519973.1"
                     /translation="MDESRKQFLEWFGEEFESINSSEELHVQAIKMIAWQSWVKSRAA
                     IEIKLDDKVMAEDDFDKGHNCAIDYCADAIRAAGIKVKE"
     gene            2665..2829
                     /locus_tag="RPM73_001601"
     CDS             2665..2829
                     /locus_tag="RPM73_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519974.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            2810..3250
                     /locus_tag="RPM73_001602"
     CDS             2810..3250
                     /locus_tag="RPM73_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDX6519975.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKAA"
     gene            3247..3837
                     /locus_tag="RPM73_001603"
     CDS             3247..3837
                     /locus_tag="RPM73_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine-binding protein"
                     /protein_id="HDX6519976.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            3834..4007
                     /locus_tag="RPM73_001604"
                     /pseudo
     CDS             3834..4007
                     /locus_tag="RPM73_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
     gene            3974..4150
                     /locus_tag="RPM73_001605"
     CDS             3974..4150
                     /locus_tag="RPM73_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDX6519977.1"
                     /translation="MARQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            4153..4500
                     /locus_tag="RPM73_001606"
     CDS             4153..4500
                     /locus_tag="RPM73_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 domain-containing protein"
                     /protein_id="HDX6519978.1"
                     /translation="MDYSQLSDFEINKRVFKAIVGAKPLGYPHNADGRSVGNESNGNY
                     RWYDYCNNPEDAEPIIVENRIGIIPAPENGLWKAAHRKVGSDSTPYHMTQDENPLRAA
                     MIVFLMMQDANNA"
     gene            4493..4669
                     /locus_tag="RPM73_001607"
     CDS             4493..4669
                     /locus_tag="RPM73_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDX6519979.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCSDCCAELM
                     SDPNSSMYEEEDDG"
     gene            4662..5228
                     /locus_tag="RPM73_001608"
     CDS             4662..5228
                     /locus_tag="RPM73_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6519980.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHILVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            5203..5805
                     /locus_tag="RPM73_001609"
     CDS             5203..5805
                     /locus_tag="RPM73_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDX6519981.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            5802..6008
                     /locus_tag="RPM73_001610"
     CDS             5802..6008
                     /locus_tag="RPM73_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HDX6519982.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            5986..6651
                     /locus_tag="RPM73_001611"
     CDS             5986..6651
                     /locus_tag="RPM73_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDX6519983.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMRKLDTIGFDTQKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKDKKYIICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADSFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            6648..7271
                     /locus_tag="RPM73_001612"
     CDS             6648..7271
                     /locus_tag="RPM73_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDX6519984.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQLGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            7705..8028
                     /locus_tag="RPM73_001613"
     CDS             7705..8028
                     /locus_tag="RPM73_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDX6519985.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            8012..8488
                     /locus_tag="RPM73_001614"
     CDS             8012..8488
                     /locus_tag="RPM73_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDX6519986.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            8485..8952
                     /locus_tag="RPM73_001615"
     CDS             8485..8952
                     /locus_tag="RPM73_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDX6519987.1"
                     /translation="MSRITAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSGASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRTLQEYIRTQCLR"
     gene            8940..9092
                     /locus_tag="RPM73_001616"
     CDS             8940..9092
                     /locus_tag="RPM73_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519988.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLDIEHLA
                     RSKGLR"
     gene            9297..9782
                     /locus_tag="RPM73_001617"
     CDS             9297..9782
                     /locus_tag="RPM73_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDX6519989.1"
                     /translation="MNNFVEITSRIGRMYQDFLISGKGSGDIIEEIDKLSAELRRNGY
                     VNSIFFETLLKQGFMFDMINYNKVAPSASQKSYVYVLHAEDSGLTKIGFSRRVNKRIS
                     EISRMSGGKLNLIAKIPADRELETKLHQKYYNYRSHGEWFSLNRCHLKELKEMPGNEL
                     K"
     gene            9949..10134
                     /locus_tag="RPM73_001618"
     CDS             9949..10134
                     /locus_tag="RPM73_001618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519990.1"
                     /translation="MEEVAMVSVNKDPKEGVEYITGADGVKRPMAYYKAAEERARIEN
                     PPKCGSFFDMLDLQWKL"
     gene            10274..10516
                     /locus_tag="RPM73_001619"
     CDS             10274..10516
                     /locus_tag="RPM73_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HDX6519991.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            10552..11040
                     /locus_tag="RPM73_001620"
     CDS             10552..11040
                     /locus_tag="RPM73_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDX6519992.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            11018..12517
                     /locus_tag="RPM73_001621"
     CDS             11018..12517
                     /locus_tag="RPM73_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDX6519993.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            12518..14683
                     /locus_tag="RPM73_001622"
     CDS             12518..14683
                     /locus_tag="RPM73_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDX6519994.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEKKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            14697..15608
                     /locus_tag="RPM73_001623"
     CDS             14697..15608
                     /locus_tag="RPM73_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDX6519995.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSHAVAEQGRKTQEFTQQSA
                     QYVEAARKHYDAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KLMEAAANKGDVETYRKLKAQLNKGIR"
     gene            15608..16903
                     /locus_tag="RPM73_001624"
     CDS             15608..16903
                     /locus_tag="RPM73_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDX6519996.1"
                     /translation="MALNEGQLVTYALDEIIETVQNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLSGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     TGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            16947..17534
                     /locus_tag="RPM73_001625"
     CDS             16947..17534
                     /locus_tag="RPM73_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6519997.1"
                     /translation="MSVMIFQAGGDTKIWGRKLKTKTVDPDDVAVHLANGWYKHPDDV
                     PDNPLVGDQIGSVGGGETSPVDMGEVSDGYHTFNELYAHRVRLFSSLMHAYAELSWWS
                     RKHSDGEEWDGWIIAGITTPEGEITYHLPVEEIEFLPEGTELEFGKEWDGHEANDVLG
                     RLLSLRPAIAEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            17512..18012
                     /locus_tag="RPM73_001626"
     CDS             17512..18012
                     /locus_tag="RPM73_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HDX6519998.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     TKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            18012..19430
                     /locus_tag="RPM73_001627"
     CDS             18012..19430
                     /locus_tag="RPM73_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDX6519999.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKKVGRIRKNI
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            19430..20278
                     /locus_tag="RPM73_001628"
     CDS             19430..20278
                     /locus_tag="RPM73_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520000.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTTNRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            20278..20733
                     /locus_tag="RPM73_001629"
     CDS             20278..20733
                     /locus_tag="RPM73_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDX6520001.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQIYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            20736..21428
                     /locus_tag="RPM73_001630"
     CDS             20736..21428
                     /locus_tag="RPM73_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDX6520002.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAW
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            21438..22826
                     /locus_tag="RPM73_001631"
     CDS             21438..22826
                     /locus_tag="RPM73_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HDX6520003.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALT
                     ARGQNITMRGQDLSASTARRGQDLAMQRASTRGTAGNDERTVQLSDGRTVTVGGKLHG
                     AGANAFYEGIDNEGNMVRVPASSIAAPATSSASAQNYAMKKDIDAIANADASALDFMT
                     GMTGGAGNPAIGADVRSRLTGKEQRQLYNSAQRIQGRMQNQGVAAARDMGASGINTVA
                     EAKMYFQGMPQVDYSSPEAMQQSIREIQEYTNNYNQQYNVNVGKSQRQQSQPAQVSQP
                     AASSNFSSLWGD"
     gene            22826..24823
                     /locus_tag="RPM73_001632"
     CDS             22826..24823
                     /locus_tag="RPM73_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDX6520004.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVDTQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAHGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPDGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRAQMRNGIIGKAMEKSGGSPDQFLRQINILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(24914..25399)
                     /locus_tag="RPM73_001633"
     CDS             complement(24914..25399)
                     /locus_tag="RPM73_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520005.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGQGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(25470..25736)
                     /locus_tag="RPM73_001634"
     CDS             complement(25470..25736)
                     /locus_tag="RPM73_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDX6520006.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LTKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            25858..>26272
                     /locus_tag="RPM73_001635"
     CDS             25858..>26272
                     /locus_tag="RPM73_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520007.1"
                     /translation="MSDITANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGSLQFN
                     FPNVLKYDPDQLRQEISTSSGASLIGSSNGKTVQEN"
BASE COUNT         7387 a         6088 c         6767 g         6030 t
ORIGIN      
        1 tattagagag tgccacagca gacaaaaagt ctgacaagaa aaaaccttcc gttagctgtc
       61 aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc gccaagaatc cgcgcactga
      121 atgacaagcg taaaaaccag atccgaacgt tctggcgcaa agccggagtg ataacccgcc
      181 agcttgacgg gcatgggttc acgatgcagg actggagaga ttatttgagc tacgtaggcg
      241 aaaattgccg atggatgttc gaagaacgcc caaaccatca acgcggaacc gtctggcaca
      301 aaaagggatt tgatttcctg cttaacgata atacctacct gaaagttcgt gagggtgaac
      361 acgatgaccg ataattttta tgcgccgccc catagcatcg aggcagagca ggcggtgatt
      421 ggtggattgc ttctggatga tgacagcagt gagcgcgtcc agaaagttct ggcgatgctg
      481 aagcctgatt cattttacgt ccgaccacac aaaatccttt tcgaagaaat aaccagaatg
      541 caccgggagc aaaagccagt agatggcctg acgcttttcg atgaactgga gcgtaaatcg
      601 ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga tcgcaaagaa cacgccaagc
      661 gcagcaaaca tcgttgccta tgcaatgcag gttcgtgaaa ccgcaatgga acgctacgcc
      721 atcaaccgca tgactgaagc gacggaattg ctatattccc gcaacggaat gactgcaacg
      781 cagaagtacg aagctattca ggcgattttc acgcaactga cagaccatgc aaaaaccgga
      841 tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag actgggttag cgaccttgag
      901 aagcgatttg acccgtcagg cgagcaacga ggaatgagca cagggatccc atcgctggac
      961 aggatgctgt caccgaaagg tctggtgaaa ggctctctgt ttgtcattgg cgcccgccct
     1021 aagatgggga aaacgacgct atacagccag atggcaatca actgcgcagt gcatgagaaa
     1081 aagcccgctc tgatgttcag ccttgaaatg cctggtgacc agatactgga aaaactggtg
     1141 ggacagaagt caggtgttaa cccgaatatt ttttaccttc cggcgacaaa tgacgctgat
     1201 gacggctatc agggtgatta cgatggtgac ttcaacaggg cgatcgaaac agccaatcgc
     1261 ttaagtgaaa tcgacctgct ttacatcgac gacacgccgg gattatctct ggctcaaatc
     1321 gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg ttggcatgat tctggtcgat
     1381 tacctgacac taatgaccgc tgagaaggcc gatcgcaacg accttgctta cggcatgatc
     1441 accaaaggac tgaagaacct tgccaaagag cttgattgcg ttgttgtgct tctgacacag
     1501 cttaaccgcg cattggaaag ccgaaccaat aaacgcccat taccaagtga ctcgcgcgat
     1561 acaggacaga ttgaacagga ttgcgattat tgggtgggga tccatcgtga aggtgctttt
     1621 gatgacagtg ttccacctgg tgaaaccgaa ctaatccttc gtctcaatcg tcatggcaat
     1681 accggcacgg tgtattgcat acaggcaaat ggcgctattt atgacacaga ccaacagtct
     1741 gctgaaatgc gccgccgtga acgcgaggaa ccgcagtcca agaagaaagg aggattctga
     1801 tgaccatcta catcactgag ctaataacag gcctgctggt aatcgcaggc ctttttattt
     1861 gtgggagagg gaagtgtggc tgactggcaa attccaatca tcattcttgc cggagcttcg
     1921 ctggttgctg gctttatcct gctgaaaaag cataaagacc gtgatcaaaa agtcgaagtt
     1981 ctctatgggt atccagcgaa cagcacaaca tggctgacca tttaccacta ccgaaaatca
     2041 ggacgctggg tattcgaatg ggatgacctg ttcgctgaaa agcgaccaaa gtcatgggga
     2101 gacatcagcg aatgcatgat gtttgaagaa agaaaatctg gcgcaaccag agatgagttt
     2161 aacgaagcgt gggcgcgatt aagtgagaga gggtatttgt gagcaagtac gaaaaattag
     2221 atcaaaacat tctttcaatg ctgagtgaaa gaccaacacc tgtttttgat atctggctta
     2281 aatggcggag caatggaatg tatatcgaaa ccatcgatcg ccgtatgcaa tacctgagaa
     2341 agaaagggct tgttgcaaat gtgcgtggga agggttgggt gaaaattaac ctgtcataac
     2401 ggggattgat atggacgaat caagaaagca gtttttggag tggtttggtg aagagttcga
     2461 gtctattaac agcagcgaag aacttcacgt tcaggccatc aagatgattg cctggcagtc
     2521 atgggttaag tctcgcgcag ctatcgagat aaaactcgat gacaaagtga tggctgagga
     2581 tgatttcgac aaaggacaca actgcgctat cgattattgc gctgacgcca tccgcgccgc
     2641 tggaatcaaa gtgaaggagt gagtatgagc gcatacgaag aaatcatgtt agccctgcga
     2701 ttctttttcg gtgtggaaga agatgaaaac gtaaatgaga ttatcggaca agaccatgac
     2761 ccgataggga ctattgcagc tgcacttgac gattacagga gcgtaaatgg tgaggaaact
     2821 aacgtttgaa ctaagaagcc ccatccatca gcagaacgcc attcaagcta tccagcaaat
     2881 tcttccagac ccaaccaaac caatcgtagt aaccattcag gaacgcaacc gcagcttaga
     2941 ccaaaaccga aagctttggg cttgccttgg tgacgtctct cgtcaggttg aatggcatgg
     3001 tcgctggctg gatgcagaaa gctggaagtg tgtgtttacc gcagcattaa agcagcagga
     3061 cgttgttcct aaccttgccg ggaatggctt tgtggtaata ggccagtcaa ccagcaggat
     3121 gcgtgtaagc gaatttgcgg agttattaga gcttatacag gcattcggta cagaacgtgg
     3181 cgttaaatgg tcagacgaag cgcgactggc tctggagtgg aaagcgagat ggggagataa
     3241 agctgcatga acaaataccg acttatttac gcagatccac cttggcaata tcgcgacaaa
     3301 gccaacgatg gcaatcgcgg tgctggacat aaatacgatg ttatgaatgt tcaggacatt
     3361 tgccgactgc cagtatggga tttagcggat ccagaatctt gcttgttagc gatgtggtgg
     3421 gtgccgacac agccagccga agcgctaaag gtaattgagg cgtggggatt caggttgatg
     3481 actatgaaag gctttacttg gcacaaaacc aataagcaca aaggcaacag tgcgatcgga
     3541 atggggcata tgacccgggc aaatagcgag gattgcttgt ttgccgttcg agggcggttg
     3601 cctgagagaa tggacgcttc aatatgccag cactttaccg caccgagaat ggagcacagt
     3661 gcaaaaccac cgatcgtaag agacatgtta gctaagttgc ttggcgacgt gccgcgctgt
     3721 gagttattta gccgcgacaa agtgaccggg tgggatatgt ggggaaatca gtgcgactcc
     3781 gattttgaac tggctcccgg catggcgatt aaaccatgca aaatggtgat cgcatgaagc
     3841 actgttatcg atgtggagag cgaaaggaag acgatcgctt tcgaccagga cagccttact
     3901 agaatcgatg gtgtctccgg tgtgaaagaa caccaacagg ggtgttacca ctacctcagg
     3961 aaaaggagga cgtgtggcga gacagcgacg aagtatcacc gacataatct gtgaaaactg
     4021 caaatacctt ccaacgaaac gctccagaaa taaacgcaag ccaatcccaa aagaatctga
     4081 cgtaaaaacc ttcaattaca cggctcacct gtgggatatc cggtggctaa gacatcgtgc
     4141 gaggaaatga caatggatta ttcacagtta agtgattttg aaattaacaa gcgagtattt
     4201 aaagcgatag taggggcaaa accattaggt tatccgcaca acgcagatgg acggtctgtc
     4261 ggcaatgaat caaatggtaa ttatcgatgg tacgactact gcaataatcc tgaagatgca
     4321 gagccgatta tcgtagagaa cagaattggc attattccag cgccagaaaa tggattatgg
     4381 aaggcagcgc atagaaaagt tggcagtgat agtaccccat atcatatgac tcaagatgaa
     4441 aacccactcc gcgccgccat gattgtcttt ctcatgatgc aggacgccaa taatgcttag
     4501 cccatcccaa tcccttcaat accagaaaga aagcgtcgag cgggctttaa cgtgcgctaa
     4561 ctgcggtcag aagctgcatg tgctggaagt tcacgtgtgc tccgattgct gcgcagaact
     4621 gatgagcgat ccgaatagct caatgtacga ggaagaagac gatggctaaa ccagcgcgaa
     4681 gacgatgtaa ccgtaaaaga gaagatttaa ctgttaaaag gatatttgag ttactaagtt
     4741 tcgataaatc taccggggta tttagatgga aagttcccac tcagggaagg atagcattaa
     4801 atagtgttgc tggaactttt gattccaacg gttattcaat gatcatgata gatgggcgta
     4861 gatataaaac tcacatctta gttttttaca taactcataa tcgctggcct gctggtcaaa
     4921 ttgaccacgt taatggaatt aggaccgaca ataggccaga aaatttaaga gaatgcctgc
     4981 caatagaaaa ttcaagaaat ataaggatcc gaaagaacag caaatcaggt tgcagaggag
     5041 ttacttggca caaacgacag aaaaaatgga atgttaggct aggtttccat ggcaagagta
     5101 aacacttcgg atgctttgat gatcttgagt tagcggtact agttgctgaa gaagcccgag
     5161 ataagtatta cggtgatttt tccggcaacg aaaggagcac ttatgcgaat ctatcgaagg
     5221 aaatgtaaat gttgcaatga atggtttata ccaaaatatc aaaatcaata ttggtgtaat
     5281 gagatttgtg gaacaaagat agcactcgaa cgacgaagta aagaacgcga aaaagcggaa
     5341 aaagcagcag agaagaaacg acgacgagag gagcagaaac agaaagataa acttaagatt
     5401 cgaaaactcg ccttaaagcc ccgcagttac tggattaaac aagcccaaca agccgtaaac
     5461 gccttcatca gagaaagaga ccgcgactta ccatgtatct cgtgcggaac gctcacgtct
     5521 gctcagtggg atgccgggca ttaccggaca actgctgcgg cgcctcaact ccgatttgat
     5581 gaacgcaata ttcacaagca atgcgtggtg tgcaaccagc acaaaagcgg aaatctcgtt
     5641 ccgtatcgcg tcgaactgat tagccgcatc gggcaggaag cagtagacga aatcgaatca
     5701 aaccataacc gccatcgctg gactgtcgaa gagtgcaagg cgatcaaggc agagtaccaa
     5761 cagaaactca aagacctgcg aaatagcaga agtgaggccg catgacgttc tcagtaaaaa
     5821 ccattccaga catgctcgtt gaagcatacg gaaaccagac agaagtagca cgcagactga
     5881 aatgtagtcg cggtacggtc agaaaatacg ttgatgataa agacgggaaa atgcacgcca
     5941 tcgtcaacga cgttctcatg gttcatcgcg gatggagtga aagagatgcg ctattacgaa
     6001 agaattgatg gcagcaaata ccgaaatatt tgggtagttg gcgacctgca cggatgctac
     6061 acgaacctga tgagaaaact ggatacgatt ggattcgaca cccaaaaaga cctgcttatc
     6121 tcggttggcg atttggtcga tcgcggtaca gagaacgttg aatgcctgga attaatcaca
     6181 ttcccctggt tcagagctgt acgtggaaac catgagcaaa tgatgattga tggcttatca
     6241 gagcgtggaa acgtcaatca ctggctgctt aatggcggtg gctggttctt taatctcgat
     6301 tacgacaaag aaattctggc taaagctctt gcccataaag cagatgaact tccgttaatc
     6361 atcgaactgg tgagcaaaga taaaaaatat atcatctgcc acgccgatta tccttgtgat
     6421 gaatacgagt tcggaaagcc agttgatcat cagcaggtaa tctggaaccg cgaacgaatc
     6481 agcaactcac aagacgggat cgtgaaagaa atcaaaggag cggactcatt catctttggt
     6541 catacgccag cagtgaaacc actcaaattt gccaaccaga tgtatatcga tactggggca
     6601 gtgttctgcg gaaatctcac attgattcag gtacagggag aaggcgcatg agactcgaaa
     6661 gcgtagctaa atttcattcg ccaaaaagcc cgatgatgag cgactcacca cgggctacgg
     6721 cttctgactc tctttccggt actgatgtta tggctgctat ggggatggcg caatcacaag
     6781 ccggattcgg aatggctgca ttctgtggta agcacgaact cagccagaac gacaaacaaa
     6841 aggctatcaa ctatctgatg caatttgcac acaaggtatc ggggaaatac cgtggtgtgg
     6901 caaagctcga aggaaatact aaggcaaagg tactgcaagt gctcgcaaca ttcgcttatg
     6961 cggattattg ccgtagtgcc gcgacgccgg gcgcaagatg cagagattgc cacggtacag
     7021 gccgtgcggt tgatatagcc aaaacggagc agttggggag agttgttgag aaagagtgcg
     7081 gaagatgcaa aggtgtcggc tattcaagaa tgccagcaag cgccgcatat cgcgctgtaa
     7141 cgatgctaat cccaaacctt acccaaccca cttggtcacg tactgttaag ccgctgtatg
     7201 acgctctggt ggtgcaatgc cacaaggaag agtcaatcgc agacaacatt ttgaatgcgg
     7261 tcacacgtta gcagcatgat tgccacggat ggcaacatat taacggcatg atattgactt
     7321 attgaataaa attgggtaaa tttgactcaa cgatggataa atgcactcgt taaataaagc
     7381 cctgagttaa tagctcgggg ctttttgcgt tttaagcacg gccattctga aagcacatca
     7441 aaccaaatac cagacagacc aaaataatca ccttatccgc tgtggctacg gtgcggtgtg
     7501 ctttgcataa aagaaaacca gcgcaatggc tggcttcgtg aaagcgggtg gcatgaggtt
     7561 gcgctaacaa cctcctgccg ttttgcccgt gcatatcggt cacgaacaaa tctgattact
     7621 aaacacagta gcctggattt gttctatcag taatcgacct tattcctaat taaatagagc
     7681 aaatcccctt attgggggta agacatgaag atgccagaaa aacatgacct gttagccgcc
     7741 attctcgcgg caaaggaaca aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt
     7801 cgcggcagat ataatggcgg tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc
     7861 attatcgcct ggttcattcg tgaccttctc gacttcgccg gactaagtag caatctcgct
     7921 tatataacga gcgtgtttat cggctacatc ggtactgact cgattggttc gcttatcaaa
     7981 cgcttcgctg ctaaaaaagc cggagtagaa gatggtggaa atcaataatc aacgtaaggc
     8041 gttcctcgat atgctggcgt ggtcagaggg aactgataac ggacgacaga aaaccagaaa
     8101 tcatggttat gacgtcattg ttggcggaga gctattcact gattactccg atcaccctcg
     8161 caaacttgtc acgctaaacc caaaactcaa atcaacagca gccgggcgct atcagcttct
     8221 ttcccgttgg tgggatgcct accgtaagca gcttggcctg aaagacttct ctcccaaaag
     8281 tcaggacgct gttgcgctgc agcagattaa ggaacgtggc gctttaccga tgattgatcg
     8341 cggtgatatc cgtcaggcaa tcgaccgttg cagcaatatc tgggcttcac tgccgggcgc
     8401 tggttatggt cagttcgagc ataaggctga cagcctgatt gcaaaattca aagaagcggg
     8461 tggaacggtc agagagattg aggtatgagc agaataaccg cgattatctc cgctctggtt
     8521 atctgcatca tcgtctgcct gtcatgggct gttaatcatt accgtgataa cgccatgacc
     8581 tacaaagagc agcgcgataa aaaagtcagt gagctgaagc aggcgaccgc caccattact
     8641 gacatgcagc aacgccagcg tgctgctgat gcactcgatg ctaaatacac gaaggagtta
     8701 gctgatgcga aagctgaaaa tgatgctctt cggcgcaagc ttgataatgg tggcagggtg
     8761 ctcgtcaaag gaaaatgccc tgtgccatcc tcagccgaaa cctccggcgc ctccggcatg
     8821 ggcaatgatg ccaccgtcga actctctcca gttgctggac gaaacgttct cggtatccgg
     8881 gacggaatca tcagcgacca aacagcactg agaacgcttc aggaatacat caggacgcaa
     8941 tgccttcgat gatagcgata attttactca tcatccttca catctggctc tgtagacagg
     9001 gtggtgatca cttctggagt gaatccagat taaacatctc attgctgatg cttgatattg
     9061 agcatctggc gcgcagtaag gggctgcgtt gagataagag ccagtcatta caaataccag
     9121 gatttagcct cgcatttgcg gggctttttt acatctgcag taaaccgcgc atcgcagcgc
     9181 gtaacaatcc cgagtctttc agaaagctga gcctgagaat tgccgtatat ggtggcgacc
     9241 atctcgggga cggcttttct gtgcgaacag gctcatcttt ctaaaaggta aacgctatga
     9301 ataactttgt tgaaattacc tcaagaattg gtcgcatgta ccaagatttt cttataagtg
     9361 gaaaggggtc tggcgacatc atagaggaaa ttgacaagct aagtgcagag ctgagaagga
     9421 atgggtatgt taattctatc ttttttgaaa ctttgctaaa gcaaggcttc atgtttgaca
     9481 tgattaatta caacaaagtc gcacccagtg cttcgcaaaa atcatatgtg tacgttctgc
     9541 atgctgaaga tagtgggcta acaaaaatag ggtttagtcg cagggttaat aaacgaattt
     9601 cagagatatc tcgcatgagt ggtgggaagc taaatctaat tgcaaagatt ccggccgata
     9661 gagagcttga aaccaaattg caccaaaaat attacaacta taggtcgcac ggggagtggt
     9721 ttagcctcaa tcgttgtcat ttgaaagagc taaaagaaat gcctggtaac gaactgaaat
     9781 aatccccggg ctcactaatt aacggcagta cagcgaaaca acccaagcca gaaagtgggg
     9841 aaataacact ggcagccact gaaagatgaa cctccagcct tatggcaaaa aagattcttt
     9901 gtggtggcgg actgatggaa agacatccgc tgaatcgatg ataaacaagt ggaagaggtt
     9961 gcgatggttt ccgtgaacaa agatccgaag gaaggcgttg aatacatcac tggtgctgat
    10021 ggtgtgaaaa gaccaatggc ttattacaaa gcggctgaag agagggcaag aatagaaaat
    10081 ccccctaaat gcggatcatt tttcgacatg ctggaccttc aatggaagtt gtgaacaact
    10141 aacaggtcgc tcaggcggcc ttttttattg ccatcacaaa agccattccc tacagagtgg
    10201 ctttggtaat ggcttatacc ctatacggga taacttaact gatatccctt ttaacggata
    10261 aacggagcca acaatggcag agattattcc catgactgaa gaacagaaat tccagttaga
    10321 gatttacaaa ctggtcatga accagaacgc agccgcagaa gaagcatttc aattcattgg
    10381 cactgacgaa ctgaagcttg agctattcaa aattcacttc cagtcaggcg gcgctaattc
    10441 agatatcacg acccgcacta tcgaagcggt gcgtaaatct aaggaagcgt tagacctgtt
    10501 cactaccgga gcatgatgct caacctgaaa taacaactaa gtgagatgaa tatggcagca
    10561 ccaaagggca accgattttg ggaggcccgc agtagtcatg ggcgaaaccc taaattcgaa
    10621 tcgcctgagg cgctgtgggc tgcttgttgt gaatacttcg agtgggctga tgataacccg
    10681 ctatgggagg gtaaggtatt ttcatatcag ggagaaataa ttaaggctaa tgtccctaag
    10741 atgcgagcca tgactatttc aggattgtgt accttccttg atatcaccag gcaaacatgg
    10801 ggaaccttcc ggtcaatgga aggtttttct gacgtcacat cacgagcgga agacatcatc
    10861 tacgaccaga aattctctgg cgcagccgct gaccttctca acgctaacat catcgcccgt
    10921 gatttgggcc tcaaagagca gtcgcaagtt gaagacgtga cacctgataa gggagatcgc
    10981 gataagcggc gctctcgtat caaggagcta ttcaaccgtg gaactggacg cgattcttga
    11041 taacctgagc gacgaagagc aaatcgaatt gctcgagcta ctcgaagaag aagagaacta
    11101 ccggaacaca cacctgctat atgaatttac gccatacagc aaacagcgtg agttcatcga
    11161 cgccgggcat gactatccag agcgctgttt tatggctggt aaccagcttg gtaagtcatt
    11221 tactggtgct gccgaagtcg cgtttcacct taccgggcgt tatccgggaa caaaaggcta
    11281 tccggctgat ggtaaatatg gcggggagtg gaaaggtaag cgtttctatg agcctgttgt
    11341 cttctggatt ggcggcgaga caaacgagac tgtaaccaaa actactcaac gcatcctgtg
    11401 cggtcgtatc gaagagaatg gtgagcctgg ctacggttcc atacctaaag aagacatcat
    11461 tagctggaag aagtctcctt tctttccgaa ccttgttgat catcttctgg ttaagcatca
    11521 cacggctgat ggcgttgaag atggcatttc aatctgctac ttcaaaccat actcgcaagg
    11581 ccgtgctcgc tggcagggtg acacaatcca tggcgtgtgg tttgacgaag aaccaccata
    11641 cagcatttat ggcgaaggcc ttacccgtac caacaaatac gggcaattct caattctgac
    11701 gtttaccccg ctgatgggga tgtctgacgt tgttaccaag ttcctgaaga atcccagcaa
    11761 gtcgcagaaa gtggtcaaca tgaccatcta cgacgctgag cactacaccg acgagcaaaa
    11821 agagcaaatc atcgcatcct atcccgagca tgagagagag gcgcgtgctc gcggtattcc
    11881 tacgatgggt agcggtcgaa tcttccagat accggaagag acgattaagt gtcagccgtt
    11941 cgagtgtcct gatcacttct acgtaattgg cgggatggat ttcggatggg atcacccaca
    12001 ggcgcaggtt cagctttggt gggataagga cgcagacaca atctacgttt cacgcgtgtg
    12061 gaaggcgaaa gaaaaaacag ccgttcaggc gtggggagca gttaaatcat gggcgcataa
    12121 agtgccaacc gcatggcctc atgacggaaa ccagcacgag aagggcggcg gtgagcagct
    12181 caaagggcag tatgccgacg ctggttttat gatgttgcag gagcatgcga catggcctga
    12241 tggcggtaac gctgtggagc ctggcatcac tgaattgcgc gacatgatgc tcgatggtcg
    12301 cttcaaagta ttcaacacct gtgagccatt ctttgaggag ttccgcctct atcaccgtga
    12361 tgaaaacggg aaaatcgtca agcttaacga cgacgttctc tcagccgttc gctatgcata
    12421 catgatgcgc cgcttcgcca aaatgatgcg cgacatcaaa aaaccaaaag agaaaaagat
    12481 accagcccca atcaggccca tcgcacggag aacttaaatg gccgacgaaa acagactcaa
    12541 ttccattctg tgtaagtttg acgcggactg gatggcgagc gatgaagcca gaaccgaggc
    12601 gacaaatgac ctgtatttta gccgagtgtc gcaatgggat gactggctat caaactacac
    12661 caccctgcaa tatcgcggac aattcgatgt tgtccgcccg gtggtaagga aactggtcgc
    12721 agagatgcgc cgtaacccta tcgacgttct cttcagacca aaagacggcg ctaatcctga
    12781 tgctgccgat gtgttgatgg ggatgtatcg tactgatatg cgccataaca cggcaaagat
    12841 tgctgttaac gttggcgttc gtgagcagat agagtccggc gttggtgcat ggcgtctggt
    12901 cacgcagtac gaagacaacg acccaacaag caacaatcag gtaatccgac gccttccaat
    12961 ccatgaagcc tgctcacacg tcatatggga cgccaacagc aagcagatgg ataagagcga
    13021 cgctaagcac tgcacggtga ttaacgcttt gtcacgcaat ggctggaaag agttcgcaga
    13081 ggattacggt attgatcctg acaccctgcc atctttccag aatccgaacg atacatggct
    13141 gtttccgtgg gtatcgaatg atgtcgtcta cgtcgctgag tattacgagg tcgaagagaa
    13201 gaaagagaaa gtcttcatct accgcgaccc gctgacaggt gagccggtca gctattacca
    13261 gcaggatatc aaagacgtca tcgacgacct ggctaatcgt ggattcatta aggtagcaga
    13321 gcgtaaggtc aagcgtcggc gtgtgtataa gtcgatcatc acctgcacgc agatactgaa
    13381 agaccgcgag aagatagccg gagagcatat cccaatcgtt cctgtgtacg gcgaatggtc
    13441 attcgctggt gacaaggagt gctacgaggg cgtggtaagg ctgacgaaag acggtcaacg
    13501 ccttcgtaac atgatcatgt cattcaacgc cgatattgtt gctcgctcac cgaagaagaa
    13561 accgaccttc ttccctgagc aaatcgaagg ctacgaatac atgtacggtg gaaatgatga
    13621 ctatccgtac tatctgcaga acaggaccga tgaaaacggt aacgacctgc cgattggtcc
    13681 aatctcctac atggaaaacc ctgaagtgcc gcaagccaac gcttacatgc ttgaggcagc
    13741 caccaacgca gtgaaagagg tggctagtct tggcgttgat gcgcaggcgg caaatggtca
    13801 ggtcgctttc gataccgtca atcaactgaa catgcgggca gatcttgaga catacgtgtt
    13861 tcaggataac ctggctaccg caatgcgacg tgatggcgag atttatgcct caatggtcaa
    13921 cgatatttat gacgttcctc gtcatgtaac gctgacactt gaagatggta gcgagaaaga
    13981 cgttcaacta tacgcgcaag ttgtagatta ccagtccggt aatgtggtca cactcaacga
    14041 cattcgcggt cgctatgagt gctatacaga cgttggacca tccttccaga gtatgaagga
    14101 acagaaccgc gcagagattc aggagttgct caccaaggtt ccgcaaggta ctccagagtt
    14161 ccagatgctg atgctgcaat acttcacgct gcttgacggt aaaggcgtcg agatgatgcg
    14221 agagtacgcg aacaagcaac tggtgatgat ggggctgaag aagccagaaa cacctgaaga
    14281 gatggagatg gtacagcagg ctcaacagca gccgcagcag ccatcagcag agcaaattca
    14341 ggcgcagggt atccttctgc aaggtcaggc tgaattgctc aaggcagaga accaacaggc
    14401 gcagattcag gttgaagctg ccaaggttga agcacaaaac caactcaacg cggcgaagat
    14461 tgcggaaatc ttcaacaata tggacctcga caagcaggca gaactgcgtg agtacctcaa
    14521 gctcgtaggt caattccagc aacagcgcag caaagatgct cgtgctaacg ctgagctgct
    14581 tcttaaagat gcagaccaga ctcattcaca acgcatggat ttcgcgaatc ttatgcgtca
    14641 agttcaaatc ccctccggcg gagtagccga gacacctcaa taagagagag ttaatcatgg
    14701 accaaaccac cgacattcag gcttctgaag aattaaccct gcccggcaat catgcagcgg
    14761 catctgctga tggcttagtt gtcgataatg ccaacgacaa cgcaggtcag gaagaaggct
    14821 tcgagattgt cctgaaagac gatgagaaac caaaacaaga cccggcaact aatgctgaat
    14881 ttgcccgtcg ccgcatcgaa cgcaaacgcc agcgtgagct tgagcagcag atggaagcgg
    14941 ttaagcgggg agagttgccg gagcacctgc gggtgaaccc tgagttacca aaacaaccag
    15001 accctaacga ttatctttcc gaagatgcac tggctaagta cgactatgac cagagccgcg
    15061 cactggctgc cttccagcag gcaaacagtg aatggcagat caaggctatg gacgcacgaa
    15121 gccatgctgt cgccgagcag ggtcgcaaaa ctcaggagtt cacccagcaa tcagcgcaat
    15181 acgtcgaggc agcccgtaag cactacgacg cagcggaaaa gctcaatatc cctgactatc
    15241 aggagaaaga ggatgcattc atgcaactgg tgccgccagc agtcggtgcc gacatcatgc
    15301 gcctcttccc ggagaaatcc gctgctctca tgtatcacct tggtgctaat cctgagaaaa
    15361 cacgccagtt gctggcgatg gacgggcaat ccgcactgat tgaactcact cgactgtcag
    15421 aacgtttaac tctcaagcct cgagccaagc ctgtttcaga agccccgtta cctgatgaac
    15481 ccattcaggg acatgctgtt gctgcaaata tctctgcgat tgaaaagctg atggaggcgg
    15541 cagcaaacaa aggggatgta gagacgtacc gcaagctcaa ggcgcaactg aataaaggaa
    15601 ttcgataatg gcattaaatg aaggtcaact ggtcacgtat gctctggatg aaatcatcga
    15661 aaccgtccag aacctgacgc caatggcgtc caaagtgaca aaatacaccc ctccagcaga
    15721 atccatgcag cgttcaagca acaccgtgtg gatgcctgtt gagcaggaag cgccaaccca
    15781 gactggctgg gatttaactg gcaacgcaac cgggattctg gaactctccg tgaaatgcaa
    15841 catgggcgat ccggataacg atttcttcga gcttcgtgca gatgacctgc gtgatgagcg
    15901 ttcttaccgt cgccgcatcc aggcatccgc caaaaaactg gcgaataaca ttgagtcagc
    15961 aattgccaaa caggcaactg aaatgggctc gcttgttgtt cacgataccc gcgcaattgg
    16021 tccatctact ggcctgtctg gctgggattt tgtgtctgat gcagagcgcc tgatgttctc
    16081 ccgtgagcta aaccgcgata tgggcatcag ttacttcctg aaccctgacg attaccgcaa
    16141 agcaggccgc aacctggtag atggtgacat ctttgggcgc gttcctgaag aagcgtatcg
    16201 caacggtact attcagcgtc agattgctgg ctttgatgaa attcttcgct caccgaaact
    16261 tccggcagtt accaagtcaa ccgctactgg tgtaactgtt tctggtgcgc agaagtttaa
    16321 gccgcaggca tacacccttg ataccgatgg taacaaagag aacgtcgaca accgtgttgc
    16381 aacggtgacc gtatcctcca ccaccgggtt taagcgcggc gacaaaatca gcttcactgg
    16441 tgtgaaattc ctgtctcaga tggcgaagaa cgtgctgact gatgatgcta ctttctcaat
    16501 cacccgtgtg atcgatggta ctcacatcga aatcacgccg aagccgattg cactggatga
    16561 cgcgtcactg acaaaagaag agaaggctta cgctaacgta aacacctctc ttgctgatac
    16621 cactccggta aacgttctga acgtggcaac aaccaccgct aacgtgttct gggctgatga
    16681 ctcaatccgc ctgctgtctc agccgatccc ggtaacccat gaactgtttg ctggcatgaa
    16741 aacgtcttcc ttcagcattc ctggtattgg tgttaacggc atcttcgcaa cgcagggtga
    16801 tatcaatact ctgtctggta agtgccgtat tgctgtgtgg tattcagcat gtgctgtacg
    16861 accagaggca attggtgttg gtctgcctaa ccagaccgcg tgataaccag agggagcttc
    16921 ggctcccttt tctattggag ataccaatga gcgtaatgat ttttcaggct ggcggagata
    16981 ccaaaatctg ggggcgaaag ctgaaaacga aaaccgttga tcctgatgat gtagctgtgc
    17041 acttagcaaa tggctggtat aagcaccctg acgatgttcc tgataatcct cttgttggtg
    17101 atcaaattgg gagtgttggc ggaggtgaaa cttccccagt tgatatgggc gaagtgtccg
    17161 acggttatca cacttttaac gagctttacg ctcaccgagt gcgcctcttc tcatcgctga
    17221 tgcatgctta cgctgagctt tcgtggtggt ctcgaaaaca cagtgacggt gaagagtggg
    17281 atggctggat cattgctggt atcaccactc cagaaggcga aatcacttat cacctacctg
    17341 ttgaagaaat cgagttcctt cctgaaggta ctgagcttga gttcgggaaa gagtgggatg
    17401 gtcatgaagc aaatgatgtt cttggacgac tcctgagttt gcgtccggcc attgcagagc
    17461 cagaagaaaa acagcgtaaa aagcctggtc gaaaacctaa ggcggcagca gatgaacctg
    17521 acaacgaagg gtgatttagt tcttgcggca ttacgtaagc tcggtgtggc atcaaatgcc
    17581 acgttaaccg atgtcgaacc tcagtccatg gaagatggtg tcaacgacct tgaaatgatg
    17641 atggctgaat ggcttggcgg tgatgcgtca ccaggtatca acgttggcta cattttcgct
    17701 gatgcagatg tcgctccaga tccgggcgat gagcacggtt tatcaaataa cgctatcaat
    17761 gccgtcattt tcaaccttgc ctgccgcatt gctccagatt atgcgctgga agcctctaca
    17821 aaacttataa ccactgccag atacgggaaa gagcgactcg tcaaactgtc tgcaatggac
    17881 agagcaaaag ccgctaaatg taagtccggt tatccaaacc gtatgcctgt tggtagcggt
    17941 aaccagttgg cgaagtggaa tggttggaat tacttccacc gaaaggaacc ttgcgataac
    18001 gggagcgaat aatgccgatt cagcaacttc cgctcatgaa aggcgtcggc aaagactttc
    18061 gaaacgccga ctatatcgac tatctgccag tgaatatgtt ggctacaccc aaagaaatcc
    18121 tcaacagcag cggatatctt cgctcattcc cgggaattgc caaacgttct gatgtgaacg
    18181 gtgtatcgcg cggcgtcgag tacaacatgg cgcagaatgc tgtttatcgc gtgtgtggcg
    18241 gcaagctgta caaaggagaa agtgaagtcg gtgatgttgc cggaagtggt cgcgtatcaa
    18301 tggcgcatgg tcggacatcg caggcggtag gcgttaatgg tcaactggtc gagtatcgct
    18361 atgatggcac ggttaaaacc gtctcaaact ggcctacaga cagtggattc acacagtacg
    18421 agttaggttc agttcgcgac attacgcgct tacgtggtcg ttatgcgtgg tcaaaagacg
    18481 gcactgattc atggtttatc actgaccttg aagacgaatc gcatcctgac cgttacagcg
    18541 cacaatatcg cgcagaatcg cagccggacg gcatcatcgg tatcggcaca tggcgagact
    18601 tcatcgtctg ctttggttca tcgacgattg aatatttctc cctgactggc gcaaccaccg
    18661 ttggtgctgc tttgtatgtc gcacagccat cgctgatggt gcaaaaaggc atcgccggga
    18721 cctactgcaa aacgccgttt gctgactcgt atgcgttcat cagcaatcca gcaacaggtg
    18781 cgccgtctgt atacatcatc ggttccggtc aggtatcacc aatcgccagc gcgagcattg
    18841 agaaaattct ccgctcctac actgctgatg aactggctga tggcgtaatg gaatcgctgc
    18901 ggtttgatgc gcatgagttg ctgattatcc atcttccgcg tcacgtcctc gtgtacgacg
    18961 catcttcaag cgccaatggt ccgcaatggt gtgtgttgaa aacaggcctg tatgacgatg
    19021 tgtaccgcgc tatcgacttc atttacgaag gcaatcagat aacgtgcggc gataagctgg
    19081 aatcggttac tggcaaattg cagttcgata tcagcagcca gtatgggctt caacaggaac
    19141 acctgctgtt tactccactg ttcaaagcgg ataacgccag agttttcgac cttgaagttg
    19201 aatcgtcaac tggcgttgcg cagtacgccg accgcctttt tctctctgca accactgacg
    19261 gaatcaatta cgggcgtgag cagatgattg agcagaatga accgttcgtt tacgacaaac
    19321 gcgttttgtg gaagaaagta gggcgcatca ggaaaaacat tggtttcaaa ttgcgcgtta
    19381 tcacgaagtc acctgtcact ctgtctggcg ctcagataag gattgagtaa tggcggattc
    19441 gaatctcaat gtgccggtaa tcattcaggc tacacggctc gacacatcag tccttccacg
    19501 caatatcttc tcgcagtcgt atctgcttta cgttatcgca cagggcactg atgttggtaa
    19561 cgtggctaac aaggccaacg aggctggaca gggcgcttat gatgcacagg tcaggaacga
    19621 tgagcaggat gtgattctcg ctgaccatga gcagcgaatt tctgctgcgg aagcaacgct
    19681 tgttaatcat gaggagcgaa tcagccaggc agaatcaact cttcaggaac atgaaacgcg
    19741 aatcgctcag aatgaaagcg atattgcgtc gcttgatacc agagttcagt cgctggaatc
    19801 gcaggtttca gaccatgaaa cgcgcattga tgctctggag tatgccacta ctcgcaagaa
    19861 gtcagaggtt gtttactctg gcgtatctgt aaccatcccg acagcgccga ccaaccttgt
    19921 tagcctgctg aaaacgctca cgccgtcatc cggcacgttg gcaccattct tcgacaccgt
    19981 taacaacaag atggttgtgt tcaacgagaa caaaaccttg ttcttcaaac tgtctattgt
    20041 cgggacgtgg cccagcggaa ccaccaacag gtcaatgcag ctaacatttt ccggctctgt
    20101 tcctgacacg ttggtcagca gtcgtaatgc ggcgacaaca accgacaaca tcctgttagc
    20161 tacgttcttc agcgtggata aagacggctt tcttgccaca aatggcagca cgttaaccat
    20221 tcagtcgaat ggtgcggcgt ttactgccac aaccatcaag ataatcgcgg agcagtgatg
    20281 attcagttca aaccaacgcg aaacatcgac ctgatcgaag cagtcggaaa tcaccctgac
    20341 attattgccg gaagcaacaa cggtgatgga tacgactaca agcctgaatg ccgttacttt
    20401 gaggttaacg tgcacgggca gttcggcggc attgtttact atcaggagat tcagccgctg
    20461 acattcgatt gccacgccat gtacctgcca gagattcgcg gcttcagcaa ggagatcggg
    20521 ctggcgttct ggcgatacat tctgactaac accaccgttc agtgcgtcac atcgttcgcc
    20581 gcacgcaaat tccgccacgg gcagatttac tgcgcaatga ttggccttaa gcgtgtcgga
    20641 accatcaaga aatactttaa aggcgtggat gacgtgactt tttacagcgc cacacgcgaa
    20701 gaactaatcg acttcctgaa tcacgggaga tagccatgtt atatgcattt aagctgggga
    20761 gaaaactgcg cggcgaggaa ccttattgcc ctgaaaaagg cgggaaaggt ggcagttctg
    20821 ataaaagcgc taagtatgca gcagaagctc agaagtatgc cgcagacctg caaaatcagc
    20881 agtggcagac gatcatgaaa aaccttgctc cgttcacgcc gcttgcggag cagtatgtta
    20941 accagcttca gaatctttcc agtttagaag gtcaggggca ggcacttaat cagtattaca
    21001 actctcagca gtataaagac cttgcaggtc aggctcgtta ccagagtctt gctgctgcgg
    21061 aggctacggg aggacttggt tcgacagcca caagcaatca actggctacg atcgcgccga
    21121 ctctaggtca gtcttggtta tcaaaccaga tgagcaatta caacaatctg gcaaacgttg
    21181 ggcttggtgc tctgcaaggt caggcaaacg cctggcagac gtacgccaac aacatgagca
    21241 gcattgcaca gcaaagcgca gctcttgccg ctgctaatgc caacaaacca tcaagtcttc
    21301 agacagcaat tagtggcgga acgtctggtg cgattgccgg tgcaggtctt gccagccttt
    21361 tgggaacatc aacaccttgg ggcgctggca ttggtgctgg tatcggattg cttggctcgt
    21421 tgttttaagg ggtaatcatg gctacttggc aaggatcaaa tggcggattg ttggctggta
    21481 ttggcggcgt caactcaaac gctcctagcg taaatgacat cggcaatacg cttcagctta
    21541 tcaggcagaa caatgatatt gagcgttcag gcgctaacaa tgttgggctg actgctttgc
    21601 aaggcctttc aggtattgcg ggggtgtttc agcaggaaaa gcaggctcag cggcagaaag
    21661 aatttcagca ggcatacgct aatgcttatg cgtctggtga tcgcggtgct ttgcgtcagt
    21721 tggctactca atatccagac cagattgaat ccgttcgtaa aggcatggga ttcattgatg
    21781 aagaccagcg caattctatc ggcaccttag cggctggcgc acgccttgcg gcctcgtctc
    21841 cagaagcaat gcaatcatgg ctgcaaaaca acgccaatga gctggcgcgc gtcggtgttg
    21901 accctaataa cgttgctcag atgtatcagc agaatccttc aggatttggt gagtttgttg
    21961 atcaccttgg gatggctgct cttggtccta ttgattactt caatgttcag gacaagatgg
    22021 caggtcgtga gattgaccga ggcaggctgg cagagacaat ccgcagcaat caggctggcg
    22081 aggcgctaac agcacgaggc cagaacatca cgatgcgcgg tcaggactta tctgcttcta
    22141 ctgcgagacg cgggcaggat ttggcaatgc agcgagcgtc aacaagagga accgctggga
    22201 atgatgagcg tacagttcag ttatcagatg gcagaactgt aacggtaggc ggtaaacttc
    22261 acggcgctgg ggctaatgcg ttctacgaag gcatcgacaa cgaggggaat atggttcgcg
    22321 ttcctgccag ttcaatcgca gcgcctgcaa catcgtctgc atcagcacaa aactatgcca
    22381 tgaagaagga tatcgacgcg atcgcaaatg cagatgcttc tgctctcgat ttcatgacag
    22441 gaatgaccgg cggcgcaggt aacccggcaa ttggtgctga tgttcgcagc cgattaacag
    22501 gaaaagagca gcgccagtta tataactcag cacaacgtat tcagggaaga atgcagaatc
    22561 agggtgtggc ggcagcaagg gacatgggtg ccagtggtat taacaccgtt gcagaagcga
    22621 agatgtattt tcaggggatg ccgcaggttg actattcaag cccggaggct atgcagcagt
    22681 cgattcgtga gattcaggaa tacaccaaca attacaacca acaatataac gttaatgttg
    22741 gtaaatctca gcggcagcaa tctcaacctg cacaggtatc acaaccagca gccagcagta
    22801 acttttcttc actatggggt gattaatggc taaagcatgg aaagatgtta tcgcctctcc
    22861 acagtatcag gcgttagcac cagaacaaaa agcgcaggct caggagcaat acttcaatga
    22921 agtcgtggcc ccgcaagccg gagaaaatgc agagcaggct aagcaagctt tctatgctgc
    22981 ctatccattg ccatctgtgc agccagtgga tacacagcaa ccagtagcac agcaacaacc
    23041 acagcaaagt ggatttatgt ctgatcttgg tgaagcagtg aaagagactg gtcgcggact
    23101 ggtgcaggct ggcgtgaacg tggcaaacat acctgcatca gttgcagatg ctgtaacaag
    23161 cgcggcggct tgggctggcg gtaaactcgg cattggcgat ggtacatatc aaccagcgcc
    23221 acgagtaaca acgcagggat tagagcagga ctttggcctt cagcaaggcg cgctgactcc
    23281 acaaacgaca gagggaaggg tatttgctga agcattgcct tacctcactc ctgctggcgt
    23341 tgagagggcg gcaacacagg caccaacact tgctggtcga attgctcatg gggcaacacg
    23401 acttctcgca gaaaacgcag ttggatcact tgctgcaaat agtgcgaaag atgatgcgga
    23461 agcactcgcc accgatttag gcgttggtgt gttggctggc ggtgctatta acgctgccgg
    23521 acgtggatta ggtgctgctt atcgtggcgt tcgtggtgct attgcgccag aagcgcagca
    23581 ggctatcaga tttgcagagc gtgaaggagt tcctctgcac accacagacc tgttacagcc
    23641 tacttcccgc gtcggaaaaa tggcgcagac tacagcagaa aatatccctc tggctggcac
    23701 aagcggaatg agagcaacgc aacaggaagc gagaagccag ttggtgcaga gatttgctga
    23761 taaattcggt gagtatgatc cagcggttgt tattgacagc ctaaaagcga aaacatcagg
    23821 aattcgtcgt gccgccggta atcgactgga gcaggttcag aatgctatgg ctggagtaaa
    23881 cattcagcct gcgcgagcaa ttcagcagat tgatacagaa atatctaacc tgcagaagct
    23941 tggcaaggtc gctgataacg agactatttc aaaacttcag tcctatcgtg atgagcttgt
    24001 tcgcaatgct ggtcctgatg gtccggtaaa tctggatttg aagcaattaa gcgatctgcg
    24061 cagccagttc agaatggacg tgaagggtga gcgaccagtg ttaccaaacc gttccgatgc
    24121 tgccattcag cgcgtttaca aggcaatgac tgacgatatc aatggtgcca ttggtcagaa
    24181 tcttggcaac gatactctcc gtaaatatca gcaggccaat gccgtctacg ctgacgaagc
    24241 ggctaaacta aagaatacca ggctgaagaa tgttctcatg aaaggtgacc tgacgccgga
    24301 agttgtcaac aacatgctat ttagcaagaa caaatcggaa attaagacgc tgtataactc
    24361 agttggtcgt gttggcaggg cgcaaatgcg caatggcatc attggaaagg cgatggagaa
    24421 atctggcgga tcccctgacc agttccttcg gcagattaac atcctgcaaa accagactgg
    24481 catcacattt aaggggcagg acgctgctta tctgaaagga ttgaaaaatt accttcagtc
    24541 cactcagcag gcagcaaaag cggcagtaac aacaccaaca gggcagcaaa ccatcccgtt
    24601 cattatcggg tatgggacgg caatgaaccc ggcgacaact ggcgcagcgg taagctacgg
    24661 acttcttact cgcgcctatg agagcgaacc attcagaaat gcaatgctcc gaatggcaaa
    24721 caccccacgc ggatcaacag cctttgagaa agcaatgcag caggcacaaa aggcaattaa
    24781 cgctctgacg cagggggcta agtctgatgc gttgtcagaa tagcttttca aacaccagga
    24841 acgtgcaaaa accaaatatg tagaacgcga ggtttatcgt accactctgc ataggcaata
    24901 cctttgctga ttgttatctt atgttactgc tactgttgca tgtgactgta tttccaaacc
    24961 ctgaattgca gtttgtatat gtgtcaacgc gtgttgggta aggttgagtt ataacaggct
    25021 ggcgcgcttt ttgctcgatc gcttgcattg tgtttacagc ctgataattc aataaagcct
    25081 gctggaatgc ttggctttgt gctatttgtt gggcttgttc ttggctttgt aattgaacat
    25141 aaagattctg aagctcaagt cttgcctgtg cgtcacttat cttgccttca tcgacacctt
    25201 gcccgagcat ctttgcagca aggacataca gcttaggtgt tggtgctgat gccatgcgtg
    25261 agtcgttctt cacactggca tcaaggcaat tagccatatc gctaagcttt tgatagcgtt
    25321 gttcgcaact tgcttgatag tcacttactt ttgcgcatcc aaccagcaga agcgggataa
    25381 ttaacagtga ttttttcata tggttaactc tccttagttt ttcacaggat atcatgaagg
    25441 caatgccatt ttatccggaa actagatttc tatgtttcct ttttattatt gctatacatg
    25501 gtcttaagcg tttcaaaaac cattttctta accatatcag attgttgttc tgccatacgc
    25561 tctgcatcgt caatgtaaac ggatgcagag ctttgtttag tcaacgattc ttcaatcgct
    25621 gcaattatct ctgagttcag cgatctgtta ttcatcttcg cacgctgctt aattttcgcg
    25681 tggagttcat gcggaagtct caagtgaaac tgcgcctcgt cgtatttgct gtacatcctt
    25741 gatgcctcac cagttgggtg gaatggcatc gtaacctact ggataaatac tcaatagtac
    25801 catttcggta tgcaatcaca tcatggttgc atcatatcat tcgtctggag taatgaaatg
    25861 tcagatatca ccgcaaatgt tgtggtaagc atgccttcgc aactcttcac tatggctcgt
    25921 tcttttaaag ccgtagccaa tggcaaaatt tatattggta aaattgacac tgaccctgta
    25981 aatcctgaaa accagattca ggtttatgta gagaacgaag atggatctca cgttcctgtt
    26041 tcgcaaccaa tcatcattaa cgccgctggt taccctgtat ataacggaca gattgccaaa
    26101 ttcgtaactg tgcaaggcca ttctatggct gtttatgatg cgtatggttc tctgcagttc
    26161 aattttccta atgtattgaa gtacgatcca gatcagttaa gacaagagat ttccacttcg
    26221 tcaggggcat cacttattgg ctcaagtaat ggtaaaacag tacaagaaaa ta
//
LOCUS       DAPPNJ010000048        26076 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000048 DAPPNJ010000000
VERSION     DAPPNJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.048"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="RPM73_001636"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="RPM73_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDX6520008.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="RPM73_001637"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="RPM73_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDX6520009.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="RPM73_001638"
     CDS             complement(941..1408)
                     /locus_tag="RPM73_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDX6520010.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="RPM73_001639"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="RPM73_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDX6520011.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="RPM73_001640"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="RPM73_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDX6520012.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="RPM73_001641"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="RPM73_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDX6520013.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="RPM73_001642"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="RPM73_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDX6520014.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="RPM73_001643"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="RPM73_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDX6520015.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="RPM73_001644"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="RPM73_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDX6520016.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="RPM73_001645"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="RPM73_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDX6520017.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="RPM73_001646"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="RPM73_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDX6520018.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="RPM73_001647"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="RPM73_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDX6520019.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="RPM73_001648"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="RPM73_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDX6520020.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="RPM73_001649"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="RPM73_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDX6520021.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="RPM73_001650"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="RPM73_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDX6520022.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="RPM73_001651"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="RPM73_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDX6520023.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="RPM73_001652"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="RPM73_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDX6520024.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="RPM73_001653"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="RPM73_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDX6520025.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="RPM73_001654"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="RPM73_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDX6520026.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="RPM73_001655"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="RPM73_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDX6520027.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="RPM73_001656"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="RPM73_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDX6520028.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="RPM73_001657"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="RPM73_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDX6520029.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="RPM73_001658"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="RPM73_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDX6520030.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="RPM73_001659"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="RPM73_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDX6520031.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="RPM73_001660"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="RPM73_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDX6520032.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="RPM73_001661"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="RPM73_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDX6520033.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="RPM73_001662"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="RPM73_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDX6520034.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="RPM73_001663"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="RPM73_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDX6520035.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="RPM73_001664"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="RPM73_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDX6520036.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="RPM73_001665"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="RPM73_001665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDX6520037.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="RPM73_001666"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="RPM73_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDX6520038.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="RPM73_001667"
     CDS             15548..15916
                     /locus_tag="RPM73_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDX6520039.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="RPM73_001668"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="RPM73_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDX6520040.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="RPM73_001669"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="RPM73_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDX6520041.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="RPM73_001670"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="RPM73_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDX6520042.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="RPM73_001671"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="RPM73_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDX6520043.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="RPM73_001672"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="RPM73_001672"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDX6520044.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="RPM73_001673"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="RPM73_001673"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDX6520045.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLINTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="RPM73_001674"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="RPM73_001674"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDX6520046.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="RPM73_001675"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="RPM73_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDX6520047.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="RPM73_001676"
     CDS             22593..23066
                     /locus_tag="RPM73_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDX6520048.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="RPM73_001677"
     CDS             23095..23637
                     /locus_tag="RPM73_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDX6520049.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="RPM73_001678"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="RPM73_001678"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDX6520050.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="RPM73_001679"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="RPM73_001679"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDX6520051.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="RPM73_001680"
     CDS             25034..25291
                     /locus_tag="RPM73_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDX6520052.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="RPM73_001681"
     CDS             complement(25267..25821)
                     /locus_tag="RPM73_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDX6520053.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6705 a         6183 c         6755 g         6433 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaacagt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgcaggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcactgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacaacgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtgtt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tttacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcga aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caacgaagag aaaaaatcct gaaatt
//
LOCUS       DAPPNJ010000049        25832 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000049 DAPPNJ010000000
VERSION     DAPPNJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.049"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(68..148)
                     /gene="shoB"
                     /locus_tag="RPM73_001682"
     CDS             complement(68..148)
                     /gene="shoB"
                     /locus_tag="RPM73_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDX6520054.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(568..948)
                     /locus_tag="RPM73_001683"
     CDS             complement(568..948)
                     /locus_tag="RPM73_001683"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDX6520055.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(948..1679)
                     /gene="pdxJ"
                     /locus_tag="RPM73_001684"
     CDS             complement(948..1679)
                     /gene="pdxJ"
                     /locus_tag="RPM73_001684"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDX6520056.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1691..2419)
                     /gene="recO"
                     /locus_tag="RPM73_001685"
     CDS             complement(1691..2419)
                     /gene="recO"
                     /locus_tag="RPM73_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDX6520057.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2431..3336)
                     /gene="era"
                     /locus_tag="RPM73_001686"
     CDS             complement(2431..3336)
                     /gene="era"
                     /locus_tag="RPM73_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDX6520058.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3333..4013)
                     /gene="rnc"
                     /locus_tag="RPM73_001687"
     CDS             complement(3333..4013)
                     /gene="rnc"
                     /locus_tag="RPM73_001687"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDX6520059.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4286..5260)
                     /gene="lepB"
                     /locus_tag="RPM73_001688"
     CDS             complement(4286..5260)
                     /gene="lepB"
                     /locus_tag="RPM73_001688"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDX6520060.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5276..7075)
                     /gene="lepA"
                     /locus_tag="RPM73_001689"
     CDS             complement(5276..7075)
                     /gene="lepA"
                     /locus_tag="RPM73_001689"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDX6520061.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7273..7752)
                     /gene="rseC"
                     /locus_tag="RPM73_001690"
     CDS             complement(7273..7752)
                     /gene="rseC"
                     /locus_tag="RPM73_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDX6520062.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7749..8705)
                     /gene="rseB"
                     /locus_tag="RPM73_001691"
     CDS             complement(7749..8705)
                     /gene="rseB"
                     /locus_tag="RPM73_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDX6520063.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8705..9355)
                     /gene="rseA"
                     /locus_tag="RPM73_001692"
     CDS             complement(8705..9355)
                     /gene="rseA"
                     /locus_tag="RPM73_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDX6520064.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9388..9963)
                     /gene="rpoE"
                     /locus_tag="RPM73_001693"
     CDS             complement(9388..9963)
                     /gene="rpoE"
                     /locus_tag="RPM73_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDX6520065.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9960..10115)
                     /gene="rseD"
                     /locus_tag="RPM73_001694"
     CDS             complement(9960..10115)
                     /gene="rseD"
                     /locus_tag="RPM73_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDX6520066.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10371..11993
                     /gene="nadB"
                     /locus_tag="RPM73_001695"
     CDS             10371..11993
                     /gene="nadB"
                     /locus_tag="RPM73_001695"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDX6520067.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPIPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11978..12715)
                     /gene="trmN"
                     /locus_tag="RPM73_001696"
     CDS             complement(11978..12715)
                     /gene="trmN"
                     /locus_tag="RPM73_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDX6520068.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12847..14181
                     /gene="srmB"
                     /locus_tag="RPM73_001697"
     CDS             12847..14181
                     /gene="srmB"
                     /locus_tag="RPM73_001697"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDX6520069.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKVKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14214..15095)
                     /locus_tag="RPM73_001698"
     CDS             complement(14214..15095)
                     /locus_tag="RPM73_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520070.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
                     EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVGNELASGELIELPFGEQTQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFVAG"
     gene            15198..15785
                     /gene="eamB"
                     /locus_tag="RPM73_001699"
     CDS             15198..15785
                     /gene="eamB"
                     /locus_tag="RPM73_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDX6520071.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15840..16223)
                     /gene="grcA"
                     /locus_tag="RPM73_001700"
     CDS             complement(15840..16223)
                     /gene="grcA"
                     /locus_tag="RPM73_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDX6520072.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16528..17217
                     /gene="ung"
                     /locus_tag="RPM73_001701"
     CDS             16528..17217
                     /gene="ung"
                     /locus_tag="RPM73_001701"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDX6520073.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17265..18302)
                     /locus_tag="RPM73_001702"
     CDS             complement(17265..18302)
                     /locus_tag="RPM73_001702"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDX6520074.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18509..18928
                     /gene="trxC"
                     /locus_tag="RPM73_001703"
     CDS             18509..18928
                     /gene="trxC"
                     /locus_tag="RPM73_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDX6520075.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18997..19695
                     /gene="tapT"
                     /locus_tag="RPM73_001704"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18997..19695
                     /gene="tapT"
                     /locus_tag="RPM73_001704"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDX6520076.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19727..22387
                     /gene="pat"
                     /locus_tag="RPM73_001705"
     CDS             19727..22387
                     /gene="pat"
                     /locus_tag="RPM73_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDX6520077.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRHNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22501..23856
                     /gene="pssA"
                     /locus_tag="RPM73_001706"
     CDS             22501..23856
                     /gene="pssA"
                     /locus_tag="RPM73_001706"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDX6520078.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23902..24225
                     /locus_tag="RPM73_001707"
     CDS             23902..24225
                     /locus_tag="RPM73_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDX6520079.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24222..25520)
                     /gene="kgtP"
                     /locus_tag="RPM73_001708"
     CDS             complement(24222..25520)
                     /gene="kgtP"
                     /locus_tag="RPM73_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDX6520080.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         6235 a         6816 c         6456 g         6325 t
ORIGIN      
        1 ttatttatag ttattaaagg cgcgtccgat tggttcaccg gacgcacctt aagtacgttt
       61 ccttgtgtta taagaacaga aggatcagct gtaaaacagc aatgatgatt ttgatgaccc
      121 gtttaatcag gtatcggcaa tcagtcattc gtttttcctt aaacaaggaa tgcagctgtt
      181 cagattagcc cttacatctc cccaaaactg aacgtgcgag ttattgaggg tgcatgctgc
      241 actccacacc agagctttga cgacaccact cgtttcaatg ggggaattct gtggcatggt
      301 gtaaagcaca gcaaaatctt caataacgaa gccaatttta atgtacttac gaattggcgg
      361 tcacgttaat ctttccatca atattgcttt cttcgtaaag gctcgagttt ttatgctaaa
      421 gattgcaagt tgcttgtaaa agataagtac actgatccat aatcgctgtt gttgagggtg
      481 catgctgcac aaaattaaag ttaaaaagta aaacccccgt tccttaccag ttcgggggtt
      541 ttacttttta aagagaacgg tattatttta actttcaata attaccgtgg cacaagcata
      601 gtggcgttca tctgccagcg ttacatgcat atttacaacg ccgagctttt ccgccagttt
      661 taatgcctca ccccatagcc gtagccgtgg tttgccgagc tcatcattga atacttcaaa
      721 ttgattaaac gccagaccat tgcggatccc ggtgccaaac gcttttgctg cggcttcttt
      781 cacagcaaaa cgcttcgcca gaaaacgcac cggctggtgg tgcgttttcc agattgccca
      841 ttcgttatcg cttaatacgc ggcgtgccag gcgctcaccg gatcgggcga tcactgcttc
      901 tatgcgagcg atctctacga tatccgtgcc taaacctaat attgccatta gccacgcgct
      961 tccagcatca gacgcttcat ttctgccacc gcatctttca gtccggtcat cactgcacga
     1021 ccaataatgg catgaccgat attcagttca tgcatctcag ggatggcggc aatagctttc
     1081 acgttgtgat aggtcagacc gtgaccagcg ttaactttca gaccgagact agcggcaaag
     1141 gtcgcggctt tggcgatacg cgccagctct tgcgcctgtt cggcgtcagt tttggcatca
     1201 gcatagcaac cggtgtggat ctcgataaac ggtgcgccaa cctctgccgc agctttgatc
     1261 tgctcttcat cggcgtcgat aaagagagaa acctggatcc cggcatctgc cagacgtttg
     1321 caggcatcgc gcattttgtc acgctgccct gcgacgtcca ggccgccttc ggttgttact
     1381 tcctgacgct tttccggtac cagacagcaa aaatgtggct tcgtctcaac ggcgatcgcc
     1441 agcatctctt cggtcaccgc catctccaga ttcatgcggg tatccagcgt ctgacgcagg
     1501 atgcgcacat cacggtcagt aatgtggcgg cgatcttcac gtaaatgcac ggtaatgccg
     1561 tccgctcccg cctgctcagc aataaacgcc gcctgcaccg gatccgggta agcagtaccg
     1621 cgtgcgttac gcagcgttgc aatatggtca atgttgacgc ctaacagtaa ttcagccatg
     1681 acaatcctca tcattcataa tgtgttttca ccgttcgctt aggcataaac tgccggaaca
     1741 gttccctgct ctttaatggt ttaccgccaa gatacggctt aagcgccatg cgggtaaagc
     1801 gtttcgcggc gcgtagtgtg tctgcgtcag gaaattcccg cgcgtttaac gcttttaact
     1861 gccttccggt gaacgtttta ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc
     1921 gataacgata cgtcatggtg tcatctaccg gctcgccgct acccgcacaa tgggtaaaat
     1981 tgacgccata gcccagatgc ccgagcagtg ccaattcaaa gcggcgcagc gcgggttctg
     2041 gcgtaccagt ggcacctgca agagactgaa tgcagtgcaa gtaatcgaaa aagagttcag
     2101 agaagcgcgt ctcgtattcc agtacgcggg agagaagttc gttgatgtac agaccgctgt
     2161 aaagcgtgat accgcttaat ggcagcgcca gcgagacagc ttcagcactg cgcagcgttt
     2221 tgacttcgcc acgcccgccg aaacgtagca agagaggggt gaaaggctgt aatgcacctt
     2281 tcagagtaga gcgtttagag cgtgcgcctt tggcaaccag acgcacgcga cccgattcct
     2341 ccgtgaagac gtccagcatc aggctggttt cgctccacgg gcgactatgc aggacaaatg
     2401 cgcgctgcca gccttccatc ggagtgactc ttaaagatcg tcaacgtaac cgagactgcg
     2461 cagtgcgcgt tcgtcgtcgg cccaaccgga tttcactttt acccacagtt caaggtggac
     2521 aggcgcttcg aacatttcct gcatgtcttt acgcgcttca atcccgatgg ttttgatctt
     2581 ggcccctttg ttgccaataa ccatcttctt ctgcccttca cgctcaacga gaatcaaacc
     2641 attgatgtca taaccaccgc gttcgttaga gacgaaacgt tcgatctcca cggtcacgga
     2701 gtacggcagt tcagcgccga ggaaacgcat cagtttttcg cggatgattt cagacgccat
     2761 aaaacgctgt gagcgatcgg tgatgtaatc ttccgggaag tgatgggtcg cttcagggag
     2821 gtgcttacgc acgattgctg cgatagtgtc gacattcagc ccggtttcag cagagatcgg
     2881 cacgatatcg aggaagttca tctgacttgc caggaactgt aagtgcggca gcagatcggc
     2941 tttttcctgc acgttatcca ctttgttcac cgcgaggatc accggcgctt tgccatcgcg
     3001 cagtttgttg agcaccattt cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat
     3061 caccagctca acgtcgccaa tagagctgct cgccgctttg ttcatcaggc ggttaatagc
     3121 gcgtttttct tccatatgca ggccaggagt atcaacgtag atcgcctgat acgcgccttc
     3181 agtatggatc cccacaatgc ggtgacgagt tgtttgcgct ttgcgggagg tgatggagat
     3241 cttctgcccc agcagtttgt tcaacaatgt ggatttgcca acgttcggac gtccgacgat
     3301 ggcaataaat ccgcagtaac ttttatcgat gctcattcca gctccagttt tttcaacgcc
     3361 tgttcggcgg cagcctgctc agccttacga cggcttgaac ctgtgccaac caccggttca
     3421 ctcaggccgc tgacctggca gtggatagta aattcctgat cgtgcgcttc gccacgtacc
     3481 tggactacca gataagtcgg cagcggcaga tggcgaccct gcaaatattc ttgcaagcgc
     3541 gttttcggat ctttttgttt atcgcctggg ctaatttcgt ccaaacgagt ttgataccag
     3601 ttgaggatta atttctcgac ggtttgaata tcactgtcga ggaatacgcc accaattaat
     3661 gcttcgacgg tgtcggcgag aattgactca cgacgaaatc caccgctttt aagttcacct
     3721 ggccctaaac gtaagcactc gcctaactca aattcgcgcg ccagttccgc cagcgtattg
     3781 ccacggacca gcgtggcgcg catccggctc atatcgcctt catccacacg agggaaacgg
     3841 tgataaagcg cattggcgat aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc
     3901 tcgttatgtt tactgctggc actacgatga gttaatgcct gctgcaacag ttcctgatga
     3961 ttaaaagtgt agcccagctt ccgttgaagc cgattaatta cgatggggtt catgcgatac
     4021 caataaataa atgcgtcaac aattcagcac acgaaacaga cctgatatac atggctctgc
     4081 taactgcttc gctgcagttt cagtttaccg gtatatgggg accaacgctg tttcgtgtgc
     4141 cgtggcaacc tggaggtgcc aaccttaaac ttcgggggaa tattctatac acaacgacgg
     4201 gggatgtcgt tagccacggg agatttatct cataaataat tcacgttgtc gccataacgg
     4261 cgacaacgtg aacgaagatg agctattaat ggatgccgcc aatgcgactt aagcgcacac
     4321 cagtcggcca ttcgccttct tgcttatcga agctcatcca gatagccgtt gcccgaccga
     4381 ccagattcgc ttcaggcaca aacccccaat aacggctgtc cgcgctgttg tcgcggttgt
     4441 cgcccatcat gaagtattgt cccggaggaa caatccaggt tgccagttgt tgccctggct
     4501 gctggtaata catccccacc tgatcctgcg caatcggcac tgtcagaatt cggtgcgtca
     4561 catcacccag tgtctcttta cgctcggaaa gacgaattcc attttctttg gtttcgtttt
     4621 tcggcacttc aaagaatccg ctggtcgctt ccccaccatt acggcgtgag aaggtctgaa
     4681 cgaaatcgct cggttccacg ttggagtagg tgaccggtag cgcgttttca cacgcctggc
     4741 cggaactgca tcccggttga atcgtcagct cttttgagac cggatcgtaa gtgactttat
     4801 cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt tggatcttcc ggatatttaa
     4861 agaccacgat atcgccgcgt ttcggatgac cggtttcgat cagcgttttc tggtagatag
     4921 gatctttaat gccataagca aacttctcta ccagaataaa atcaccgatt aacagagtcg
     4981 gcatcatcga acctgacggg atctggaacg gttcataaat aaacgaacgt acaatcaata
     5041 cgatagccag caccggaaaa acagaagcac cggtttccag ccagccaggc ttcggcgcaa
     5101 cctttttcag ggttgcttta tccagtgagt caccggcagc cgcctgcgcc gctgcctgac
     5161 gttcccgccg tttaggtgcg aaaaagaatt tatccacgca ccataaaatg cccgtcacca
     5221 gtgtggcaat caccagaatc agggcaaaca tattcgccat gccaactcct aagggttatt
     5281 tgttgtcttt gccgacgtgc agaatggcga ggaacgcttc ctgcggcagc tcgacgttac
     5341 cgatctgctt catgcgtttc ttaccttctt tctgcttctg cagcagcttt ttcttacggc
     5401 tgatatcgcc gccataacat ttagccagta cgtttttacg cagctgtttc acggtggatc
     5461 gcgcaataat gtgcgtacca atcgctgcct gaatggcgat atcaaactgc tggcgtggga
     5521 tcagatcttt catcttctcc accaactcgc gaccgcggtt ttgcgaatta tcacggtggg
     5581 tgatcagcgc caacgcatca acacgttcac cgttgattaa tacgtctaca cgtaccatgt
     5641 cggacgcctg gaagcgtttg aagttgtaat ccagagacgc ataaccacgc gaggtagatt
     5701 tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg gatctcgtac gtcagcgcca
     5761 cctgattacc gtggtaaacc atattggtct gcacgccgcg tttttctacg cacagcgtaa
     5821 taacgttgcc gagatatgcc tgcggcagca gcatgtgaca ttcggcaatc ggctcgcgca
     5881 gttcgtagat gttatttacc gctggcagct tggatggact gtcgacgtaa ataacttctc
     5941 ttgacgtggt ttcaacttca tacactacgg tcggcgcagt ggtgatcaga tccagatcgt
     6001 attcacgttc cagacgttcc tggatgatct ccatgtgcag caggccgagg aagccgcagc
     6061 ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata gaacagtgaa gcatcgttca
     6121 ggctgagttt acccagcgcg tcacggaagg cttcatagtc gtcggaactt accgggaaca
     6181 gaccggcgta tacctgcggt ttgactttct taaagccagg cagcgccttt tctgccggat
     6241 tacgcgccag cgttaaggta tcgccgactg gagcgccgtg gatatcttta atcgcacata
     6301 ccagccagcc tacttcgcca catttcagtt cagtgcggtc aacctgcttc ggcgtgaaga
     6361 tgcccagacg gtcggcgtta taggtctgcc cggtactcat gactttcact ttgtcgccct
     6421 tacgcagggt gccgttttta atacggataa gtgaaacaac gcccagatag ttgtcgaacc
     6481 aggagtcgat aattagtgcc tgcaacgggc cttccggatc gccttccggc ggcggaatgt
     6541 cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc ggttttcgct gaacagcgca
     6601 ccgcgtcggt ggcgtcgatg ccgacgatat cttcaatttc ttccgccacg cgttcaggat
     6661 cggctgccgg caggtcaatc ttgttcagta ccggcaccac ttcgagatcc atttccatgg
     6721 cggtgtagca gtttgccagg gtttgcgctt ctacgccctg cccggcgtcg accaccagca
     6781 atgcaccttc acaggcagcc agcgaacggg aaacttcata ggagaagtct acgtggcccg
     6841 gggtgtcgat aaagttaagc tgataggttt cgccgtcaga cgctttgtag tccagcgtca
     6901 cgctttgcgc tttgatggta atgccacgct cacgctcaag atccatggaa tcgagaacct
     6961 gcgcctccat ttcacggtca gacaggccac cgcagatctg gataatacgg tcagacagcg
     7021 tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt acgtatattc ttcataaagt
     7081 atgattattg tgccttaatg cccggttaac caggctttta gaagtcgctg ttctgagctt
     7141 aacgtctgta ttaatagaaa cgccgcattc tacactacaa cattgaggcg aggaaatgtt
     7201 cataccgtat ggattgtggt atctggaaac gtcctcgcat ttgttatgca aaatgcaata
     7261 aagccagtga aatcactggc tcgcgtcttc cgaagatgtt tcaaatcgca caaggccagg
     7321 cggcaatgcc acgcttaaga tgatcggttg ccattctgcc cgggcagcaa acttacgcga
     7381 gtagccgcgc gcaatcagga acccgccaat accaccgagg atcgcaccac ataatgctgc
     7441 gacgtcagaa gcaaagagta gctgaaatag cgaagcgatg aggaataatc ccaccagcgg
     7501 cgacatataa accagtaatg cggagctaag caggctgcct tcggcgatcc ccaattccac
     7561 tttttgcccc ggcactaacg gctcatcaca gggtacgaca atggtatgcg tggtttgcgg
     7621 gccaagttta tttaacacgc ggctaccgca accggcgcgt gaagcgcagc tgctgcatga
     7681 agctttaaca tcacaactga ccagcgcctg cccgttttgc caggagacga cggtagccca
     7741 ctctttgatc attgcgctgc cccgaactta atattctcgg caatgcgttt cgccgtttgc
     7801 ggcggcagtt caccgacaat ggtgatttcg gcgttatcac gtacgcttgt actgacggtt
     7861 ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg tagcgcggtt aacgtttacc
     7921 gagaagctga ataatccgtc ggaatagaga cgtgattcga taggcatgtt gtccatcgtc
     7981 ggtagcggac gtcgactact ggaaacttcg ctaaaaccct gtggcaacca ggttggcgtc
     8041 cagctgaatt tagctttttc acctacagga acagaaagca acggcggcaa atttgccttc
     8101 gccagcgtct gcatactgct gctgatatcc tgattgacgt taaaagcaat cacgcgaaat
     8161 tgttccagcg tttcaccatc gcgatcaagg agatcaaccc gcatcggtaa tttcgattcg
     8221 gtgtccatcc acacgatgta gctgtagcgt gtaccatctc gggcaaccac gcgaatgact
     8281 tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga taaagtcgta gtaaggagaa
     8341 aggcgtttga aatcggtata gataagcgat ggcagagaat caacaatgta atcgccatta
     8401 agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt cattgccgcg ctgtaccact
     8461 tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag gacggttatc gaggcgtgca
     8521 tgtcgataac gcagagactc aacaccctgt ttattgatgc tgatgaatga cagctcgtaa
     8581 ttcagtgact gactggccag gttcatctgc tgtaataacg ccccggacgc gggagtggcc
     8641 gaggcgttag cagagaataa caggctacct gtcactaatg acatggcaaa ccaaagttgc
     8701 ttcattactg cgattgcgtt cctaaagttt gaattcctgg cacctgtaca gcggcttgct
     8761 gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag tcggcgttgc agttcgtaat
     8821 cctgcaacat tgcattaatg cgacgacgct gctcctgtac ctgctgctgt tgaccattgt
     8881 ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct ggctttaccc atcatcggca
     8941 gtgtattaaa taccggcgtt tcgggctgct gggacgtttc agattgtcca ttatagtgct
     9001 ggacgccaac gataactgca agcgatacgc atgctgctac gcccatttgg gtaagctgtg
     9061 ccgcccacgg acgtactttc tgccagaatg gcattttctg ccattgatgc ggcgcaggct
     9121 gggcttccgg gatcaatgtc gccggttgac gtactggctc ttcttcaatg gcggccatca
     9181 cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc accccgcatt gagtcacgga
     9241 ttaagtgata gctttcccag gttttctgca tttctgggtt atgagccagt tcgttaagca
     9301 gctcactatc cagcgtttcg ccatccatta aagcggaaag ttgttctttc tgcatgccta
     9361 atacccttat ccagtatccc gctatcgtca acgcctgata agcggttgaa ctttgttatc
     9421 aatagcttcc ctcgctcgga agatacgtga acgcaccgta cctaccggac aatccatgat
     9481 agcggctatc tcttcatagc tcaggccatc cagctcccgc aaggttattg ccatgcgcaa
     9541 atcttccggg agggactcaa tagttcggaa aactatctgt ctcagttctt ctgacaacat
     9601 taagttctca gggttcgaaa tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc
     9661 aatggcatcc acatcactgg aaggtggacg acgcccctga gcaaccaggt aatttttcgc
     9721 tgtatttaca gcaatccgat acagccatgt ataaaaagcg ctatctcccc ggaacgaatc
     9781 cagcgcacga taggctttaa taaaagcttc ttgtaccaca tcgggaacat cacccgacgg
     9841 cacatagcgg gaaaccagac tcgccacttt atgctgatag cgcactacca gtaggttaaa
     9901 ggctttctga tctcccttct ggacccgttc aaccaggacc tggtccgtta actgctcgct
     9961 catccgaggt aaagtctccc caaaccaaat ttccacgcgc tatcgaaacg ccactccatt
    10021 agctgcaatt tgagcaagca aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc
    10081 atctgttttt gtttgtcatg ctgtagacgg atcattatct atcattataa gtctacagaa
    10141 tctgaacatc gcattatctg tgtagaaatg cccatttaac tgcctgaaga gtaacccaac
    10201 ggccttttta tttcaccacc taatcctcca ccagccagta acttctcttt ttctcgccgc
    10261 cctgcgtcag cgtgtttagc cactgtaaca aatattaaaa tagcaggtgt ttattcgcac
    10321 aacatgatgc tatgctgacc aaacagtgtt tagtaaatta aacaaagaaa atgaatactc
    10381 tcccagaaca ttcatgtgac gtgttgatta tcggtagcgg cgcagccgga ctttcactgg
    10441 cgctacgcct ggctgaccag catcaggtca tcgttctaag taaaggcccg gtaacggaag
    10501 gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt tgatgaaact gacagcattg
    10561 actcgcatgt ggaagacaca ttgattgccg gggctggtat ttgcgatcgc catgcagttg
    10621 aatttgtcgc cagcaatgca cgatcctgtg tgcaatggtt aattgaccaa ggggttttgt
    10681 ttgataccca cgttcaaccg aatggcgaag aaagctacca tctgacccgt gaaggtggac
    10741 atagtcaccg tcgtattctt catgccgccg acgccaccgg tagagaagta caatccacgc
    10801 tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct ggagcgcagc aacgcggttg
    10861 atctgattgt ctctgacaaa attggcctgc cgggcacgcg acgggttgta ggcgcgtggg
    10921 tatggaaccg aaataaagaa acggtggaaa cctgccacgc aaaagcagtg gtactggcaa
    10981 ccggcggtgc gtcgaaggtg tatcagtaca ccaccaatcc ggatatttct tctggcgatg
    11041 gcattgctat ggcgtggcgc gcgggctgtc gggttgccaa tctcgaattt aatcagttcc
    11101 accctaccgc actgtatcac ccacaggcac gcaatttcct gttaacggaa gcactgcgcg
    11161 gcgaaggcgc ttatctcaag cgcccggatg gcacgcgttt tatgcccgat tttgatgagc
    11221 gcggcgaact ggccccgcgc gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc
    11281 tcggcgcaga ttgtatgttc ctcgacatca gccataagcc cgccgatttt attcgccagc
    11341 atttcccgat gatttatgaa aaattgctcg ggctggggat tgatctcaca caagaaccga
    11401 taccgattgt gcctgctgca cactatacct gcggtggtgt aatggttgat gatcatgggc
    11461 gtacggacgt cgagggcttg tatgccattg gcgaggtgag ttataccggc ttacacggcg
    11521 ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt ctatggctgg tcggcggcag
    11581 aagatatcac cagacgtatg ccttatgccc acggcgtcag tacgttaccg ccgtgggatg
    11641 aaagccgcgt tgagaaccct gacgaacggg tagtaattca gcataactgg cacgagctac
    11701 ggctgtttat gtgggattac gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc
    11761 tgcggcggat aaccatgctc caacaagaaa tagacgaata ttacgcccat ttccgcgtct
    11821 caaataattt gctggagctg cgtaatctgg tgcaggttgc ggaattgatt gttcgctgtg
    11881 cgatgatgcg caaggagagt cgtggcctgc atttcacgct ggattatccg gaactgctca
    11941 cccattccgg tccgtcgatc ctttcccccg gcaatcatta cataaacaga taaaaagcct
    12001 gggtcagcgc cgtatacgct tcggaatagt tctggtctgg cccacgaatg actaagcgat
    12061 cgctaaagca ttctcccgcc tgcggggaga atgccagcag cacccgatgc ggcagtcgcg
    12121 cttcgttttc cgccacatcc gtccgcaaac gtaaatgcca gcccatgctt aatgccagct
    12181 ccgtaaaacc attaccaatc tgctctggca gcactacgca gaaaaatccc tcttcggtaa
    12241 tgcactccgc cgcacaggtc agcagcgatg ggtgatcaag cgtagtggta tagcgagcct
    12301 gttcccgttg aggggtcgcg cactctactc cctgctgata gtaaggtggg ttactgatga
    12361 ttaaatcgaa gcgtactgtc tgctgtgtga tccactgctg aatatccgcc gtatggacgt
    12421 taatccgctc tgcccacggg gactggttga tattttcctg cgcctgcgcg gcagcttcac
    12481 tttccagctc aacggcatca attatcacgc tgtcatcggt tcgctgcgcc agcattaatg
    12541 ccagcaaccc gctacccgcg ccgatatcaa ggcaacgttt caccccagcc accggtgccc
    12601 atgcgcccag caaaatgcca tccgttccca ctttcatcgc acagcgatcg tgagcaacaa
    12661 aaaactgttt aaaagtaaat ccattacgac gaagcacgga tgtagactgt gacatgaaaa
    12721 taaaaccttg caggaaaaac ggcgatagca ccgggtgaga acaatacccg agaagcgata
    12781 tccatacaaa cagatgaaga ttgcagccgt aacgtctata atcagcgccc cacacagagg
    12841 tagaacatga ctgtaacgac tttttccgaa cttgaactcg acgaaagcct gctggaagcc
    12901 ctccaggata aaggtttcac tcgcccgacc gccattcagg ctgccgccat tccgcctgcg
    12961 ctcgatggcc gtgatgtact cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat
    13021 ctgctgccag cgttgcagca cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt
    13081 attttgatcc tcaccccaac tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa
    13141 ctggcgaaac atacgcatct ggatatcgcc accatcaccg gcggcgtagc ctatatgaac
    13201 cacgcggaag tgttcagcga aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg
    13261 caatacataa aagaagagaa cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa
    13321 gcagaccgta tgctggatat gggctttgct caggatatcg aacatattgc tggcgaaact
    13381 cgctggcgta aacagaccct gctcttttcg gcaacgctgg aaggcgatgc gattcaggac
    13441 tttgccgagc gtctgctgga ggatccggtg gaagtttctg cgaatccgtc cacccgtgag
    13501 cgcaaaaaaa ttcatcagtg gtattaccgc gccgatgatc ttgagcataa aaccgcgttg
    13561 ctggtgcatc tgttaaaaca gcctgaagcg acccgctcaa ttgtgtttgt gcgtaagcgt
    13621 gaacgtgtgc atgagctggc aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc
    13681 gaaggtgaga tggtacaagg caagcgtaac gaagcgatca agcgtttgac cgaaggtcgc
    13741 gtaaacgtac tggttgcaac cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc
    13801 cacgtcttta acttcgatat gccgcgcagt ggcgatactt atttgcaccg tatcggacgt
    13861 accgcgcgcg ccggtcgtaa aggcaccgcg atttcgctgg tggaagccca tgaccatttg
    13921 ctgctgggca aagttggccg ttatattgaa gagccaatta aagctcgcgt tattgatgag
    13981 ttacgcccga aaacgcgtgc gccaagcgag aagcagaccg gcaagccatc gaagaaagta
    14041 ctggcaaaac gcgccgagaa gaaaaaagtt aaagagaaag agaagccgcg agtgaaaaaa
    14101 cgccatcgcg ataccaaaaa tattggtaag cgccgtaaac caagcggaac gggcgtgcca
    14161 ccgcaaacga cagaagagta atctcaatgc cgggtttaag cccggcatta tttttatcca
    14221 gccacaaaac actcttcaac acactgaata aaagtgtgca tcgccggact aaccgctttt
    14281 ccggcatggt gagcgcacat tgccgtaatg gtctgcgtct gttcgccaaa aggcaattcg
    14341 attaattcgc cactcgcaag ttcatttccc actgcaaagc gcggcaggta actgaccccg
    14401 atatttgccg cgacacaacg cttgatgctt tcgatactta taagctcaat ggtgttttcc
    14461 accgtgatcc gccgctggcg cagcgtgctc tcaaatatct gccggaagac acattgtggt
    14521 tcgttgataa taaagctaca ggcgttatgt cttcccggct cagtaaaatc gatatctgca
    14581 atttgcggtg aagccaccag caccagcggt tgttcaccca actctcgtcg gttcagaaca
    14641 tcatcattcc ctacgcgata aaaaaccccg acatcggcct catcattcag tagcgcatca
    14701 cggatcacgt aacagttcag cgactgcaac gataaacgca cttttggagc gcgttgccga
    14761 aaacgctgca acacctgcgg cattcggtaa gagagcaacg tttcgcctga aacaacgcgt
    14821 agttccccgt ccggatccga ctcttttttc gcagcctcac gaagcgtatc catcactcgg
    14881 gtaagttcat agatgtgcgg cagcaacttt tttccttcgc gggtaaggca cattcgtcga
    14941 ccaattttct caaacaattg taccgagaac tcctgctcaa gctgctgaat atgaaaagtc
    15001 accgtcgatt gtgtacagca caatttttgc gaagctcgca aaaaggaacc ctcttccacc
    15061 acggttttaa gtgtaataaa acggcgcaga tccataaccc caaacctatc gaaaatatcg
    15121 aatttagaat gtaaaaacat tcattttttt aaatgttccg tgtcgggtac tgtctaccaa
    15181 aacagaggag ataacaagtg acaccgaccc ttttaagtgc tttttggact tacaccctga
    15241 ttaccgccat gacgccagga ccgaacaata ttctcgccct tagctctgct acgtcgcatg
    15301 gatttcgtca aagtacccgc gtgctggcag ggatgagtct gggatttttg attgtgatgt
    15361 tactgtgtgc gggcatttca ttttcactgg cagtgattga cccggcagcg gtacaccttt
    15421 tgagttgggc gggggcggca tatattgtct ggctggcgtg gaaaatcgcc accagcccaa
    15481 caaaggaaga cggacttcag gcaaaaccaa tcagcttttg ggccagcttt gctttgcagt
    15541 ttgtgaacgt caaaatcatt ttgtacggtg ttacggcact gtcgacgttt gttctgccgc
    15601 aaacacaggc gctaagctgg gtagttggcg tcagcgtttt gctggcgatg attgggacgt
    15661 ttggtaatgt gtgctgggcg ctggcggggc atctgtttca gcgattgttt cgccagtatg
    15721 gtcgccagtt aaatatcgtg ctcgcgctgc tgctgatcta ttgcgcggta cgcattttct
    15781 attaattaaa aaagcggaag aggtcgccct cttccgctta gtaacttgct acttaagcct
    15841 tacaggcttt cagtaaaggt acgagcgata acgtcgcgct gctgttccgg agtcagagag
    15901 ttaaagcgaa ctgcataacc tgatacacgg atggtcagct gcggatattt ttccggatgc
    15961 ttaactgcat cttccagagt ttcacgacgc agaacgttaa cgttcaggtg ttgaccacct
    16021 tcaacgcgaa cttctggttt cacttctact ggaacttcac ggtattcaat gtcacccagt
    16081 ttgcttactg caaccacttc atcttctgca taacctgctt ttgcaacgat gcaacgcgct
    16141 tcgccttttt cgctgtccag cagccagaaa gagttcagca gatcgtcgtt agcggcttta
    16201 gtaatctgga tacctgtaat catgtgatgc ctccccggca aaattatttg atttattcag
    16261 cctgtcgcgg ccaattggta aaaccattgt tgcttgagtg tatatatact cctcaacaac
    16321 ccttgattcc ttgatttaaa tcaataaaaa ccacacatcg attgtggtcg caaatggatt
    16381 ttattgtttt acatcaactt atgcgggtgt gaaattttac caatttacat ttttttgcac
    16441 tcgtttaagt ctaaaaaatg agcatgattt tgttctgtag aaataagcag ttaagctagg
    16501 cggattgaag attcgcagga gagcgagatg gctaacgaat taacctggca tgacgtgctg
    16561 gctgaagaga agcagcaacc ctattttctt aatacccttc agaccgtcgc cagcgagcgg
    16621 cagtccggcg tcactatcta tccaccacaa aaagatgtct ttaacgcgtt ccgctttacc
    16681 gagttgggtg acgttaaagt ggtgattctc ggccaggatc cttatcacgg accgggacag
    16741 gcgcatggtc tggcattttc cgttcgtccc ggcattgcca ttcctccgtc attattgaat
    16801 atgtataaag agctggaaaa tactattccg ggcttcaccc gccctaatca tggctatctc
    16861 gaaagctggg cgcgtcaggg cgttctgctg ctcaatactg tgttgacggt tcgcgcaggt
    16921 caggcgcatt cccacgccag cctcggttgg gagacgttta ctgataaagt catcagcctg
    16981 attaaccagc accgcgaagg cgtggtgttt ttgttgtggg gatcacatgc gcaaaagaaa
    17041 ggggcgatta tagataagca acgccatcat gtactgaaag caccgcatcc gtcgccgctt
    17101 tcggcgcatc gtggattctt tggctgcaac cattttgtgc tggcaaatca gtggctggaa
    17161 caacgtggcg agacgccgat tgactggatg ccagtattac cggcagagag tgagtaaatt
    17221 tgcggggaaa tgccggatgg cagagttgcc acccggctga tttatcaggc tttattctga
    17281 cgccaccatt caccaagcaa aacgccggtt gcgacagaga tattcagccc ggcaacgttg
    17341 cccgtaccgt caatcttcac gcgcagatcg ttcggatcgc gtgcggcatc cggtaaccct
    17401 tcatattcct gacccagcac cagtaccatt ttcgctggca gactagtttt gaacagcggt
    17461 ttaccctgct cgctggaagt ggtcactacg gtgtaacctg cctgacggaa atcatccagc
    17521 acgttaacaa tgttgtcgcc agtaatcggc tgaacgtgct ctgcgccgcc ttctgcggta
    17581 cggatagccg cccccgactc cagcagtgcc gcatcctgca ccacgacacc tttcacgccg
    17641 aagtgcgcgc agctgcgcat catgccaccc aggttatgcg ggttagattc gttttccagt
    17701 gccagaacgc aatcctgcgc gcctgcctgg cttacccact gctgcacggt tgtaccgtta
    17761 cgctttttga tcaagaagca aacgccgccg tgatgttccg tgcctgacgc ttttgtcaat
    17821 tccgcttcat ccaccacatg gtacgctttg cggtttgctg ccatccagcg caaggcttct
    17881 ttaaaacgcg gcgttacact ctggataaac caggcgcgaa caatcgcttc cggacggctc
    17941 tggaaaagtg cctgacatgc attttcgccg tagacgcggg tttcttccgc acgctgacga
    18001 cgcaacactt ccggatcaat aaaactttta ccactgatgc caccgtgatc ggccttttcc
    18061 ggcgtctcat cacccggcgc gcgggaaacc gtgcgccacg gcgaatcttc ccacttgcgg
    18121 tcacggggct gactctgttt gtcatcgcgg gcggggcgac ggccaccgtc agcacgagat
    18181 tttcctggac gcccgccccc tttcccggta cgcgggttgt gggtacgttt atcagaatca
    18241 tcatcactgc ggacatacat cactttgacc ttgccgcttt tacctttcat ttcatcgttc
    18301 atgcttttct ccaccagcgc tgcgcgaagc gcgcagatta cccgaagtcc gcgcggttcg
    18361 ccatgatttc gtaccaaagc ctgcgactat catacctatt gaataaaaca gattgttgtc
    18421 tggaacaatg tccccgataa tatgtaacat attagaaaca taccggcgtc gttgccgata
    18481 agtctcctta ctcatcccga ggttagttat gaataccgtt tgtacccatt gtcaggccat
    18541 caatcgcatt cccgacgatc ggatcgaaga tgcggcaaaa tgcggacgct gcggtcacga
    18601 cttgtttgac ggagaggtga ttaatgcgac cggtgaaaca ctcgacaaat tgctgaagga
    18661 tgatctacct gtggtgatcg acttctgggc accgtggtgc ggcccctgcc gtaatttcgc
    18721 accaattttt gaagatgtcg cgcaagagcg tagcggtaaa gtgcgctttg tgaaagtgaa
    18781 taccgaagct gaacgtgaat tgagcagtcg ctttggaatt cgtagtatac cgacgatcat
    18841 gattttcaaa aacggtcagg ttgtcgacat gcttaatggc gcagtaccga aagcgccgtt
    18901 cgatagctgg ctgaacgaat ctctttaatc ttaccggggc acatcttgtg ccccgttttc
    18961 tcctctgcga caatggcgtt ttttcgacgc tctcttatga ccgaaaacgc tgttctccag
    19021 ttacgcgccg agcgtattgc gcgcgcaaca cgtccttttc ttgcccgcgg taatcgcgtt
    19081 cgtcgctgcc aacgctgtct tttgccagag aaattatgtc tctgttcgac aattacccca
    19141 gcacaagcaa aaagtcgctt ctgtttgctg atgttcgaca ccgagccaat gaagccaagt
    19201 aacaccgggc gtctgattgc tgatattttg cctgataccg ttgcgtttca atggtcgcgt
    19261 accgacccct cgcaagactt gctggatctg gtacaaaacc cggactatca accaatggtg
    19321 gtctttcccg cctcgtatgc tgatgagcaa cgggaagtga tcttcacacc acctgccggt
    19381 aagccaccgc tgtttatcat gctcgatggt acctggccgg aagcgcgcaa gatgtttcgt
    19441 aaaagtccgt atctggataa tcttcccgtc atttccgtcg atctttcccg gctttctgcc
    19501 tatcgcctgc gtgaagccca ggctgaaggc caatattgta ctgccgaggt agccatcgca
    19561 ctgttagata tggccggcga taccggggcg gcagcagggt taggcgagct ttttacccgc
    19621 tttaaaacac gctatctggc aggaaaaacg caacatctgg gtagcatcac agcagaacag
    19681 ttagaaagcg tttaaaatca ttcggtcact tctgcgggag accggtatga gtcagcgagg
    19741 actggaagca ctactgcgac caaaatcgat agcggtaatt ggcgcatcga tgaaacccaa
    19801 tcgcgcaggt tacctgatga tgcgtaacct gctggcggga ggctttaacg gaccggtact
    19861 tccggtgacg ccagcctgga aagcggtgtt gggtgtgtta gcctggccgg atattgccag
    19921 cttgcccttt acacccgacc ttgcggtttt atgtaccaat gccagccgta atcttgctct
    19981 tctggaagag ctcggcgaga aaggctgtaa aacctgcatt attctttccg ccccggcatc
    20041 gcaacatgaa gatctccgcg cctgcgccct gcgccataac atgcgcctgc taggaccaaa
    20101 cagtctgggt ttactggctc cctggcaagg tctgaatgcc agcttttcgc ctgtgccgat
    20161 taaacgcggc aagctggcgt ttatttcaca atcggctgcc gtctccaaca ccatcctcga
    20221 ctgggcgcaa cagcgtgaga tgggcttttc ctactttatt gcgctcggcg acagcctgga
    20281 tatcgacgtt gatgaattgc tcgactatct tgcgcgcgac agtaaaacca gcgccatcct
    20341 gctctatctc gaacagttaa gcgacgcgcg acgctttgtt tcggcggccc gtagtgcctc
    20401 gcgtaataaa ccgattctgg tgattaaaag cggacgtagc ccggcggcac agcgactgct
    20461 caacacgacg gcaggaatgg atccggcgtg ggatgcggct attcagcgtg ccggtttgtt
    20521 gcgggtacag gacacccacg agctgttttc ggcggtggaa acccttagcc atatgcgccc
    20581 gctgcgtggc gaccggttga tgattatcag caacggtgct gcgcctgccg cgctggcgct
    20641 ggatgcctta tggtcacaca atggcaagct ggcaacgcta agcgaagaaa cctgccagaa
    20701 actgcgcgat gcactgcctg gacatgtggc aatctctaac ccgctcgatc tgcgcgatga
    20761 cgccagcagc gagcattatg tcaaaacgct ggacattctg ctccacagcc aggattttga
    20821 tgcgctgatg gttattcatt cacccagcgc agcggctccc gcaacagaaa gcgcgcaaga
    20881 attaattgaa gcggtaaagc atcatccccg cagcaaatat gtctctttgc tgacgaactg
    20941 gtgcggcgag cactcctcgc aagaggcacg acgtttattc agcgaagccg ggctgccgac
    21001 ctatcgcacg ccggaaggca ccatcaccgc gtttatgcat atggtggagt accggcataa
    21061 tcagaagcaa ctacgcgaaa cgccggcgtt gcccagcaat ctgacttcca ataccgcaga
    21121 agcgcatctt ctgttgcaac aggcgattgc cgaaggggct acgtcgctcg atacccatga
    21181 agttcagcct atcctgcaag cgtatggcat gaacacgctc cccacctgga ttgccagcga
    21241 tagcaccgaa gcggtgcata ttgccgaaca gattggttat ccggtggcgc tgaaattgcg
    21301 ttcgccggat attccacata aatcggaagt tcagggcgta atgctgtacc tgcgtacagc
    21361 caatgaagtc cagcaagcgg caaacgctat tttcgatcgc gtaaaaatgg catggccgca
    21421 ggcgcggatc cacggcctgt tggtgcaaag tatggctaac cgggctggcg ctcaggagtt
    21481 gcgggttgtg gttgagcacg atccggtttt cgggccgttg atcatgctgg gtgagggagg
    21541 tgtggagtgg cgtcctgaag atcaagccgt cgtcgcactg ccgccgctga acatgaacct
    21601 ggcccgctat ctggttattc aggggatcaa aagtaaaaag attcgtgcgc gcagtgcgct
    21661 acgcccattg gatgttgcag gcttgagcca gcttctggtg caggtttcca acctgattgt
    21721 cgattgcccg gaaattcagc gtctggatat tcatcctttg ctggcttctg gcagtgaatt
    21781 taccgcgctg gatgtcacgc tggatatcgc gccgtttgaa ggcgataacg agagtcggct
    21841 ggcagtgcgc ccttatccgc atcagctgga agaatgggta gaattgaaaa acggtgaacg
    21901 ctgcttgttc cgcccgattt tgccagaaga tgagccacaa cttcagcaat tcatttcgcg
    21961 agtcaccaaa gaagatcttt attaccgcta ctttagcgag atcaacgaat ttactcatga
    22021 agatttagcc aacatgacgc agatcgacta cgatcgggaa atggcgtttg tagcggtacg
    22081 acgtattgat caaacggaag agatcctcgg cgtcacgcgt gcgatctccg accctgataa
    22141 catcgatgcc gaatttgccg tgctggttcg ctcggatctc aaagggttag gcttaggtcg
    22201 acgcttaatg gaaaagttga ttacctatac gcgagatcac ggactacaac gtctgaatgg
    22261 tattacgatg ccaaacaatc gtggcatggt ggcgctagcc cgcaagctcg ggtttaacgt
    22321 tgatatccag ctcgaagagg ggatcgttgg gcttacgcta aatcttgccc aacgcgagga
    22381 atcatgagta aggtactgga aatgttgacc acttaatcgg gactggtgtt attattgccc
    22441 gcttatgtcg tctgcattgc acagaggacc cttcaatgaa cagagaagaa atgcactgtg
    22501 atgttgtcaa aatttaagcg taataaacat caacaacacc ttgcccaact acccaagatt
    22561 tctcaatcag ttgatgatgt cgatttcttt tacgctcccg ccgacttccg ggagacgctg
    22621 ctggaaaaaa tagccagcgc gaagcagcgc atttgcattg tcgccctgta tctcgaacag
    22681 gatgacggtg gcaaaggcat tctgaacgcg ttgtatgaag ctaaaaggca gcgtccggaa
    22741 ctggatgtgc gggtgctggt cgactggcat cgtgcacaac gtggacgcat tggcgctgcg
    22801 gcatctaaca ctaacgctga ctggtactgc cgcatggcgc aggaaaatcc gggcgtagat
    22861 gttccggttt atggcattcc aatcaatacc cgtgaagccc ttggtgttct gcactttaaa
    22921 ggctttatca tcgacgatag cgtactttat agcggtgcca gcctgaacga tgtttacctg
    22981 catcagcacg ataaatatcg ctacgaccgt tatcatctta tccgtaaccg taagatgtca
    23041 gacattatgt ttgaatgggt tacacagaat attatgaatg gccgcggcgt taatcgtctg
    23101 gatgatgtta atcggccaaa aagcccggaa atcaagaacg atattcgtct gttccgccag
    23161 gagctgcgtg atgccgctta tcatttccag ggcgatgccg acaacgatca gctttccgta
    23221 acgccgctag tggggctggg gaaatcgagt ctgttgaaca agaccatttt ccatcttatg
    23281 ccttgtgccg agcagaaact aaccatctgt acgccatact tcaacctgcc agcaatcctt
    23341 gtgcgcaata ttatccagtt gctgcgcgaa gggaaaaagg tcgaaattat tgttggtgat
    23401 aaaaccgcga acgacttcta cattccggaa gatgaacctt tcaagataat tggcgcattg
    23461 ccttatctct atgagatcaa tctgcgtcgt ttcctgagcc gtttgcagta ttacgtcaat
    23521 actgaccagc tagtggttcg gttatggaaa gatgacgaca acacctatca cctaaaaggg
    23581 atgtgggttg atgataagtg gatgttgatc accggtaata acctgaaccc gcgcgcctgg
    23641 cgtctggatc tggaaaacgc cattttgatc cacgatccgc aacttgagct ggcgccacag
    23701 cgagagaaag aactggagct gatccgcgag cataccacca tcgttaagca ctatcgcgat
    23761 ctgcaaagta ttgccgatta tccggtgaag gttcgtaaac tcatccgccg tttgcgccgt
    23821 atccgcatcg accgattaat tagccgcatc ctgtaatcac aaccccgtcc tgtacggggt
    23881 ttgttttttg gaggccacgt tttgcgtatt ctttttgtct gttcgctatt gttactttct
    23941 ggatgcagcc atatggctaa cgatagctgg agcgggcagg ataaagctca acactttatc
    24001 gcctcggcga tgctttccgc cgccggaaat gaatattcac agcatcaggg gatgagccgg
    24061 gatcgcagtg ccatgtttgg attgatgttc tctgtcagtt tgggggcgtc aaaagagctt
    24121 tgggatagcc gccccgaagg gagcggctgg agctggaagg atttggcctg ggatgtcgcc
    24181 ggtgcaagca ccggctatac cgtctggcaa ctgacccgtc actaaagacg catccccttc
    24241 cctttgcgat gtagcatcaa agaaaccaga aacgccacca cggccatcaa ggtcacatac
    24301 cagaagaagg ctgtttccat tcctattgat ttcagcgaca acgctacgta ctccgccgaa
    24361 ccaccaaata tagcattagc gaccgcatat gacaggccaa cgcctaatgc gcgaacctgt
    24421 gccgggaaca tctcagcctt cagtattcca ctgattgatg tataaaaact cactatcagc
    24481 agggcacaca tcaccagacc aaaagcggca taaggcgagg aaacgttttg caatgctgag
    24541 agaataggaa cggtaaaaat ggctgccagc gaaccgaaac ataacattga ggtacggcga
    24601 ccaatcttat ccgacagcgc gccaatgagt ggttgaataa gcatgaatac aaacaatgcg
    24661 gcagtcataa tgccactcgc cacgttggca tgcattcccg cagtatttac cagatacttc
    24721 tgcatataag tggtgaaggt atagaaacaa agggagcccg cagcggtaaa accgagaacc
    24781 atgatgaatg cacggcgatt gcgccataat cctttcagag atccagcttc ttttaaagcg
    24841 cgcgtttctt gttgcgaagt ttcatctaac tgacgacgta accacaacgc cacaacagct
    24901 aacacagctc ctaacgcgaa aggaatacgc catccccact ctctgagtgc agcgtcttcc
    24961 atggtgtgtt gtaaaaccac gacaaccagt agggctagca gttgtccgcc gatcaacgtc
    25021 acatactgaa atgatgcgta aaaacctttg cgcccttcaa cggcaacttc actcatatag
    25081 gtggcgctgg tgccatattc tccgccaaca gataatccct gaaataaacg cgcgagaagc
    25141 aataatgccg gagcccacgt acctatagtt tcataacctg ggaggcaggc gataaccagc
    25201 gatccgaaac acatcataca caccgataac agcatcgatt ttttgcgacc atgtttatcg
    25261 gctatgcggc caaatagcca accgcctatt gggcgcatca ggaatcccgc agcaaaaaca
    25321 cctgctgttt gtagtagttg agtcgtcgtg ttcccggaag ggaagaaaat gtgggcaaag
    25381 tagagtgaac agaacgagta gacatagaaa tcgaaccact cgaccagatt acctgaagag
    25441 gcccccacaa tcgcccaaat gcggcgacga gtatcactac ttgtcagttt gctgtctgcc
    25501 gttacagtac tttcagccat gccattatgt ctcctgccgt aatccgatgc ttttgtcggt
    25561 cgcttttatt tatttctttt ggtaaagaaa atgttataca tttgttgcgt atcattatgc
    25621 aaccttaacc atgaatttag ttagcaggaa aatggttatt gaggagctta aggataaatt
    25681 tctggtaagg aggacacgta tggaagtagg caagttgggg aaaccgcatc cgttgctgaa
    25741 tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttctgcgcca
    25801 acgcaaaaag gccatcgtga ctgatggcct tt
//
LOCUS       DAPPNJ010000050        25823 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000050 DAPPNJ010000000
VERSION     DAPPNJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.050"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="RPM73_001709"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="RPM73_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDX6520081.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="RPM73_001710"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="RPM73_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDX6520082.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="RPM73_001711"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="RPM73_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDX6520083.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="RPM73_001712"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="RPM73_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDX6520084.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="RPM73_001713"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="RPM73_001713"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDX6520085.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSASEIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="RPM73_001714"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="RPM73_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDX6520086.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="RPM73_001715"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="RPM73_001715"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDX6520087.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="RPM73_001716"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="RPM73_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDX6520088.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="RPM73_001717"
     CDS             complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="RPM73_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDX6520089.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9312..10157
                     /gene="glpF"
                     /locus_tag="RPM73_001718"
     CDS             9312..10157
                     /gene="glpF"
                     /locus_tag="RPM73_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDX6520090.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10180..11688
                     /gene="glpK"
                     /locus_tag="RPM73_001719"
     CDS             10180..11688
                     /gene="glpK"
                     /locus_tag="RPM73_001719"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDX6520091.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11824..12834
                     /gene="glpX"
                     /locus_tag="RPM73_001720"
     CDS             11824..12834
                     /gene="glpX"
                     /locus_tag="RPM73_001720"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDX6520092.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12931..13677
                     /gene="fpr"
                     /locus_tag="RPM73_001721"
     CDS             12931..13677
                     /gene="fpr"
                     /locus_tag="RPM73_001721"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDX6520093.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13682..14110)
                     /gene="uspD"
                     /locus_tag="RPM73_001722"
     CDS             complement(13682..14110)
                     /gene="uspD"
                     /locus_tag="RPM73_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDX6520094.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14137..14436)
                     /locus_tag="RPM73_001723"
     CDS             complement(14137..14436)
                     /locus_tag="RPM73_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDX6520095.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14648..15088)
                     /locus_tag="RPM73_001724"
     CDS             complement(14648..15088)
                     /locus_tag="RPM73_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX6520096.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15189..15788
                     /locus_tag="RPM73_001725"
     CDS             15189..15788
                     /locus_tag="RPM73_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDX6520097.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15896..16663
                     /gene="tpiA"
                     /locus_tag="RPM73_001726"
     CDS             15896..16663
                     /gene="tpiA"
                     /locus_tag="RPM73_001726"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDX6520098.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16718..17473)
                     /gene="cdh"
                     /locus_tag="RPM73_001727"
     CDS             complement(16718..17473)
                     /gene="cdh"
                     /locus_tag="RPM73_001727"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDX6520099.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGSLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17580..18569)
                     /gene="sbp"
                     /locus_tag="RPM73_001728"
     CDS             complement(17580..18569)
                     /gene="sbp"
                     /locus_tag="RPM73_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDX6520100.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18889..19851)
                     /gene="pfkA"
                     /locus_tag="RPM73_001729"
     CDS             complement(18889..19851)
                     /gene="pfkA"
                     /locus_tag="RPM73_001729"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDX6520101.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20032..20934)
                     /gene="fieF"
                     /locus_tag="RPM73_001730"
     CDS             complement(20032..20934)
                     /gene="fieF"
                     /locus_tag="RPM73_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDX6520102.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21083..21583)
                     /gene="cpxP"
                     /locus_tag="RPM73_001731"
     CDS             complement(21083..21583)
                     /gene="cpxP"
                     /locus_tag="RPM73_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDX6520103.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21733..22431
                     /gene="cpxR"
                     /locus_tag="RPM73_001732"
     CDS             21733..22431
                     /gene="cpxR"
                     /locus_tag="RPM73_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDX6520104.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22428..23801
                     /gene="cpxA"
                     /locus_tag="RPM73_001733"
     CDS             22428..23801
                     /gene="cpxA"
                     /locus_tag="RPM73_001733"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDX6520105.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23907..24581)
                     /gene="yiiM"
                     /locus_tag="RPM73_001734"
     CDS             complement(23907..24581)
                     /gene="yiiM"
                     /locus_tag="RPM73_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDX6520106.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24730..25713)
                     /gene="kdgT"
                     /locus_tag="RPM73_001735"
     CDS             complement(24730..25713)
                     /gene="kdgT"
                     /locus_tag="RPM73_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDX6520107.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         6369 a         6720 c         6654 g         6080 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta
      781 gtggaagtgc tggacgttga gccggtattt actcactccg tctggcggtt actgctctgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccaggcgg tggatctgaa cagcctgaaa cgctccccca agcaacaaca ggcgctggcg
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggtcgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctacgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacaa
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc
     1441 gagcggcttt cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac
     1561 gacagctcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
     1621 cgtgcgcata gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
     1681 ttgtgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgccg ggcggggaat
     1741 gcgcgtccgg caattcaaca cgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccaataatca ggttatcctc
     1861 tttcttaatc gtcggggttt tgcgcctgcg ctgctgtgcc acgactgcgg ctggatagcc
     1921 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagtcagcg tccagtaccg cgccagtgtc cgtcctgcgg ctctacccat
     2041 ctggtgccgg tgggtctggg caccgaacag cttgagcaaa cgctcgcgcc gctgttccct
     2101 ggtgtgccca tttctcgtat cgaccgcgat accaccagcc gcaaaggagc gctggaacaa
     2161 caactggcgg aagtgcatcg cggcggcgcg aggatcttga ttggcacgca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtagcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagagcgt ttcgcccagc tttacaccca ggtcgccggt
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2581 ccgctggcag acgacaaact gtgggttctc ggcccggttc cggctctggc acctaagcgt
     2641 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg caacctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattc gcaccggaac
     3421 tggagctacc tacagttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatc
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt
     3541 gtcactaccg cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggtaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaagttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc agcaattagc ggagcagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg
     5341 ccgttgagct ggcgaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgtg
     5521 cactgttaga aaatactgaa ctgagtgcta gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctgat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcgctga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaagcgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaactatcat cattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc acttcgatcc gctggtcgcc ctgctggcac
     7201 taattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcatgccata cgctggccga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttgagt gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaatatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaatggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctcg ctggtgtgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccattact ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccaatgct ggaacgtact gtcaagggag
     7921 cgttactgac taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacagc ctacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tctgaagatc cgctggatat tgaatgatag aaaaggcacc gcaaggtgcc
     8581 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcggaag atgaagcgta
     8641 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
     8701 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
     8761 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
     8821 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
     8881 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaatcctcgt cccgattacc
     8941 ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc
     9001 atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct
     9061 ggtgacataa tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca
     9121 aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga
     9181 agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata agtttacagc
     9241 atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc
     9301 aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg
     9361 gtaccgggtt gttgattttc ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg
     9421 cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct
     9481 acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc
     9541 tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg
     9601 ctttctgtgc tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc
     9661 agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta
     9721 cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg
     9781 ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc
     9841 ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat
     9901 tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg
     9961 cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc
    10021 ttttcggccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc
    10081 gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact ccttcagaac
    10141 aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa aaaatatatc
    10201 gttgcgctcg accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat
    10261 atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ttgggtagaa
    10321 cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa
    10381 gccgatatca gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact
    10441 attgtctggg aaaaagaaac cggcaagcct atctataacg ccattgtctg gcagtgccgt
    10501 cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgcagc
    10561 aataccggtc tggtgattga cccgtacttt tctggcacca aagtgaagtg gatcctcgac
    10621 catgtggaag gctctcgcga gcgtgcacgt cgtggtgaat tgctgtttgg tacggttgat
    10681 acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc
    10741 tctcgtacca tgttgttcaa catccatacc ctggactggg acgacaaaat gctggaagtg
    10801 ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt atacggtcag
    10861 actaacattg gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag
    10921 caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc
    10981 actggctgct ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg
    11041 ctgaccacca tcgcctgcgg cccgactggc gaagtgaact atgcgttgga aggtgcggtg
    11101 tttatggcag gcgcatccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc
    11161 tacgattccg aatatttcgc caccaaagtg caaaacacca atggtgtgta tgtggttccg
    11221 gcatttaccg ggctgggtgc gccgtactgg gacccgtatg cgcgcggggc gattttcggt
    11281 ctgactcgtg gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat
    11341 cagacgcgtg acgtgctgga agcgatgcag gccgactctg gtatccgtct gcacgccctg
    11401 cgcgtggatg gtggcgcagt agcaaacaat ttcctgatgc agttccagtc cgatattctc
    11461 ggcacccgcg ttgagcgccc ggaagttcgc gaagtgactg cattgggtgc ggcctatctt
    11521 gctggtctgg cggtcggttt ctggcagaac ctcgacgagc tgcaagagaa agcggtgatt
    11581 gaacgcgagt tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg
    11641 aaaaaagcgg tcaaacgcgc gatggcgtgg gaagaacacg acgagtaatg taaatgccgg
    11701 atgaagcgtt tatgccgcat ccggtagtcc tgaaaccgtg cgggggcaac cccgcacaca
    11761 tcaataatcc cacccttccc ctgtgctaca cttcgcgcca ttccttactg cttagagttt
    11821 gctatgagac gagaacttgc catcgaattt tcccgcgtca ccgaatctgc ggcgctggct
    11881 ggctacaaat ggttaggacg cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc
    11941 atgcgtatta tgctcaacca ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa
    12001 atcgacgaag caccgatgct ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg
    12061 gtagatattg ctgttgatcc gattgaaggc acgcgcatga cggcgatggg ccaggctaac
    12121 gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat
    12181 atggagaagc tgattgtcgg gccgggagcc aaaggcacca ttgatctgaa cctgccgctg
    12241 gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac cgttgagcga actgacggta
    12301 acgattctag ctaaaccacg ccacgatgcc gttatcgctg aaatgcagca actcggcgta
    12361 cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa ttctcacctg tatgccagac
    12421 agcgaagttg acgtgctgta cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg
    12481 gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa
    12541 ggcgacaacg aagagaatcg tcgcattggc gagcaggagc tggcacgctg caaagcgatg
    12601 ggcatcgaag ccggtaaagt attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc
    12661 ttctctgcca ccggtattac caaaggcgat ctgctggaag gcattagccg caaaggcaat
    12721 atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac gcaccattcg ccgcattcag
    12781 tccatccact atctggatcg caaagacccg gaaatgcagg tgcacatcct ctgattgatt
    12841 tgatcgattg agccttccag tccttcggga ctggaatttt tttgttcgga gaacgaagat
    12901 aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt gggtaacagg caaagtcact
    12961 aaagtgcaga actggaccga cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg
    13021 tttaccgccg ggcaatttac caagcttggc cttgaaatcg acggcgaacg cgtccagcgc
    13081 gcctactcct atgtaaactc gcccgataat cccgatctgg agttttacct ggtcaccgtc
    13141 cccgatggca aattaagccc acgactggcg gcactgaaac caggcgatga agtgcaggtg
    13201 gttagcgaag cggcaggatt ctttgtgctc gatgaagtac cagattgcga aacgctatgg
    13261 atgctggcaa ccggtacagc gattggccct tatttatcga ttctgcaact aggcaaagat
    13321 ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac gttatgccgc cgacttaagc
    13381 tatttgccac tgatgcaaga actggaaaaa cgctacgaag gcaaactgcg cattcagacg
    13441 gtggtcagtc gggaaacggc agcggggtcg ctcaccgggc ggataccggc gttaattgaa
    13501 agcggggaac tggaaagcgc gattggcctg ccgatgaata aagaaaccag ccatgtgatg
    13561 ctgtgcggca atccacagat ggtgcgcgat acacaacagt tgctgaaaga gacccggcag
    13621 atgacgaaac atttacgtcg ccgaccgggc catatgacag cggagcatta ctggtaagcg
    13681 gttacttatc gataaacggt acgatgagca aatccgcact catcttattg atcatcccgc
    13741 gatatgccgg catcaaacgg ttgataaatg agtggtgatg accacagaca aggaggtcgc
    13801 actgctcttt ttgcataatt tccagcagtg tttccggcat ttctccgcgt tcaatacgca
    13861 gttttgtctt cggccattga atatttttcg tcagtttata cagcttgtta tccgacttat
    13921 tcttcaacaa ttgaagaata tcttctgttg cagggaagta gatacccgga tacaactcgc
    13981 ttaagccatc atcaatatga attaacgtca ggtgagcgtc attatgtctg gcgagctcca
    14041 gggctttatt caccagtaag gcatcttctt cattcccgga aattgccacg ccaatgtgtt
    14101 tataagccat gtttaactcc ttctaaagca actccatcaa gctaacgaac gcagtgataa
    14161 ctcaaaaata atcatctcgg cctggcatga gaaagtgaag gccgcatcaa gctcaacttg
    14221 cccctccaga tccttaatct tataggccac gctggcccct tcatctgcag aggatgcagc
    14281 ggcggcaagc tctttcagtt tggtcataaa agactctgct tcttcgcggg tggcaaaaaa
    14341 gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc gtaccgatat caatcgcaca
    14401 tcctttagta ctgcatttat ctacgacatc tttcataggg cacctctgta tattcgcctc
    14461 tctgtcgatt acgacttaag aataatgcgc cgttaaggga agaacaaaca agtaatgatc
    14521 ttatggatta ccattttttt tgatattgct taaggtgaag agttcagaag gattttccgg
    14581 gcattgtcat ttgcatcgtg gaaataacaa tcccggaaaa aaatagcagt aagagatata
    14641 ttaaaggtta attacttgat ttattgtcgg ctttatactt cacatcctgg gtatctttac
    14701 catatttatt ttcgccttgt gtgccaatga acgctccaag atcgataagc atcatcacca
    14761 gaatcaacgt cgggacaaaa cgccccaccg cccattgcca gacacccggt aaaatcgccc
    14821 agttacccgc cagcagcatc cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc
    14881 cgcgatcatg caagcgctta acggttactg ccgctgttgg ccaaagcaag cacacaaggc
    14941 aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc cagtgagaaa agcaccagca
    15001 tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg gcgtccaata cgccctttaa
    15061 atgagaataa ccattgctgt atggtcatgt aagttccttg atggttgtct tttccaggat
    15121 tctacccttt tgacaagggc gacagatatc gttttaatcg gagccagtca taacaaaagg
    15181 tactgtcaat gaagccaggg tgtacgctgt tttttctctt atgttctgca ttaaccgtta
    15241 caacaacggc gcatgcacaa acaccagata cggcaacgac cgcgccttat ctgctggctg
    15301 gagcccctac tttcgatctc tccatcagcc agtttcgaga agactttaac agccagaatc
    15361 ctagcctgcc actgaacgaa tttcgtgcca tcgacagcag tcccgacaaa gccaatctca
    15421 ctcgtgctgc aagtaaaatt aacgagaact tgtatgcttc tacagcgctg gagcgcggta
    15481 ccttaaaaat caaaagcatt caaatgacct ggctacccat ccaggggcca gagcaaaaag
    15541 cagcgaaagc gaaagctcag gaatacatgg cagcagtgat ccgcacactc accccattaa
    15601 tgaccaaaac acaaagccag aaaaaactgc agtcgctact aacggcgggg aaaaacaaac
    15661 gttattacac cgagacagaa ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg
    15721 ggctgacctt cgctgttgaa ccgattaagc tggcgctatc tgaatcgctt gaaggtttga
    15781 ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc tatactgttt cacagacctg
    15841 ctgccctgcg gggcggccat cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg
    15901 acatccttta gtgatgggta actggaaact gaacggcagc cgccacatgg ttcacgagct
    15961 ggtttctaac ctgcgtaaag agctggcagg tgttgctggc tgtgcggttg caatcgcacc
    16021 accggaaatg tacatcgata tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg
    16081 tgcgcaaaac gtggacctga acctgtccgg cgcattcacc ggtgaaactt ctgccgctat
    16141 gctgaaagac atcggcgctc agtacatcat catcggccac tctgaacgtc gtacttacca
    16201 caaagagtct gacgaactga ttgcgaaaaa attcgcggtg ctgaaagagc agggtctgac
    16261 tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa gcgggaaaaa ctgaagaagt
    16321 ttgcgcacgt cagatcgacg cggtactgaa aactcagggt gctgcggcat tcgaaggtgc
    16381 ggttatcgct tacgaacctg tatgggcaat cggtactggc aaatctgcaa ctccggctca
    16441 ggcacaggct gttcacaaat tcatccgtga ccacatcgct aaagttgacg ctaacatcgc
    16501 tgaacaagtg atcattcagt acggcggctc tgtaaacgcg tctaacgctg cagaactgtt
    16561 tgctcagcca gacatcgacg gcgcgctggt tggcggtgcg tctctgaaag ctgacgcttt
    16621 cgcagtaatc gttaaagctg cagaagcggc taaacaggct taagtctgac aggtgccgga
    16681 tttcatatcc ggcacttact ttccttaact cctcgcctta acgcaaaatc tcacactgat
    16741 gatcctggat ttcctcggct gaagcacggt tcagcgtcag tagatttcgt tgtgtcgcca
    16801 gcaacacaaa cgaattatca ctctgccgta ccatcgccag cccgtagctt cccatatgtt
    16861 cccgcgcctc aggtacttct tctgccagca tcataaatgg gctgcgttgt accagttcgc
    16921 tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa accacctggc agtggcaacc
    16981 agcggctgct gatgttcgcc agattgttat ccagctgttc gcgcacatca ggacgaatac
    17041 aagagatatg aatatgaaaa tggttttgcg tacgtccggt gcgggagttg atcgccaaag
    17101 aaaccgcgcg atcgggaacc ggctggccgt attttttgct cataaaatca cgcgcctgcc
    17161 aggccaacca aaagaagttc ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt
    17221 taatacgata cgttggcatc aacagatatt gcagtgagcc attaagatct tttaaaacca
    17281 cgtatccggc attgggtttg acttccgcac atggcgaagg attttgattt tgctgctgat
    17341 tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc cgactcttca ccggttaatt
    17401 tccagtaacc aataccggca gccacaacgg cgataactat catcaccaaa aaaagaagac
    17461 ccgctttttt catctttttt tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg
    17521 tgacaaataa aaaacccggt cgcgagaaac caccgggttt gataattatc ctggggaaat
    17581 cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc
    17641 gtccagccgc cgaactcttc atcaatggtg aataacttca gctttggaaa cgcattttcg
    17701 tactttttcg ctacctcagc gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga
    17761 ccttctggcg agtagagata tttcaggtag gcttccgcca cctctttggt gccttttttc
    17821 tcgaccactt tatcgaccac cgacacggtt ggctctgcga ggatagactc actcggcgtg
    17881 acgatttcga atttatcttt ccccagttca ttcgctgcca gcagagcttc gttttcccaa
    17941 gcgatcagta catcgccaat cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca
    18001 gaatccagaa cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc ttttgcctga
    18061 tcgttgttgt tgtgatgcag cgcgtagccc caggcagcca ggtagttcca gcgtgcgcca
    18121 ccagagcttt tcggattagg cgtgatcacc gaaacacccg gtttaatcag atcgttccag
    18181 tcatggatct gcttcggatt gcccttacgc accaggaaaa caatggtgga agtgtacggt
    18241 gcggagttat ccggcagacg tttgatccac tctttatcaa tccgcccgcg ttccgcaatt
    18301 gcgtccacgt cataggccag agccagagtg acaacatcag cttcaatacc attgattacc
    18361 gacgtcgctt gtttgcctga accaccgtgt gactgacgaa tcaccacgtt atcgccagtt
    18421 tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga
    18481 tcatatgaaa cgttaagaag ctgaatatcc tttgccataa cgctggttgc cgccagcaaa
    18541 aatgttaacc ctacgcccca cttgttcatc gcccgactct cttatgttgt gttgtgatga
    18601 gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa aaaaagattg gctataactt
    18661 gcgggtatat gttgggggat taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag
    18721 tgcgctttgt ccatgccata tgcggcgcga acgccttatc cggcctacaa aagtttgcaa
    18781 attcaataaa ttgcagaatt catgtaggcc tgataagcga agcgcatcag gcatttttgc
    18841 ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt aatacagttt
    18901 tttcgcgcag tccagccagt cacctttgaa cggacgcttc atgttttcga tagcgtcgat
    18961 gatatcgtgg tgaaccagct gttcgttctg gatacctaca caacgaccgc cgtaacctgc
    19021 cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt cgtaaggcac
    19081 cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac gaccggtttc
    19141 tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa tcgccacgat
    19201 cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt cttcacggct
    19261 gaattcaact tccggaacta caacgaattc acagccaccg gcaatggccg cagccagggt
    19321 cagatcgcca caataacgac ccatcacttc caccacggaa atacgctggt gagaagaaga
    19381 ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga agaaaccgat
    19441 agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa
    19501 gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga taaccaccag
    19561 cgcgtcgata ccacgttttt tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg
    19621 gaattccggg aagcgcgcag aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac
    19681 gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc cgtcataaat
    19741 gcccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt
    19801 catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt ttcttaatca tgactacctc
    19861 tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat
    19921 ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca ggtcaggcca
    19981 aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg attatgaaag
    20041 catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga taattacatc
    20101 cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca tatgtgcctg
    20161 aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg tcggccctga
    20221 ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca cgatatcaat
    20281 aatttcttgc cgttcctcat caggcaatgc gcgatccagt aatgactgta ccgcctcata
    20341 tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa acagagcatc
    20401 ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc agaattgcgc cgttcatcat
    20461 aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac
    20521 cacccaacgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa ttgtcacgat
    20581 aaccccgacg cctggatctg tcatcggtgt tggagatatc agatgttgaa tacccgtcaa
    20641 aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg cgagggactc
    20701 tgctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat atcgcaccac
    20761 cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg cggcgagaat
    20821 actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca acgaagccat
    20881 cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat tcataaatac
    20941 tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc attaagcagc
    21001 aggcaaattg aggataaaaa aaacccccac atcatgtggg ggaagacagg gatggtgtct
    21061 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
    21121 acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt
    21181 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
    21241 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
    21301 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
    21361 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
    21421 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt
    21481 cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt aatgaactga
    21541 ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc tcccaaaatc
    21601 tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat gcgtcagggg
    21661 gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt ctgcctcgga
    21721 ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct gacttcccta
    21781 ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga tggggaacag
    21841 gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat gatgccgaag
    21901 aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc tgtcattatg
    21961 ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg cgcagatgac
    22021 tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc
    22081 cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac actggaagtt
    22141 gatgccttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca aacgctggag
    22201 ttaaccggta ctgagtttac cctgctctat ttgctggcac agcatctggg tcaggtggtt
    22261 tcccgtgaac atttaagcca ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct
    22321 attgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg
    22381 tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg ataggcagct
    22441 taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg atgttggttt
    22501 tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc gaacagcgtc
    22561 aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg cccaacgatt
    22621 taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt
    22681 tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa atgcagatca
    22741 ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa aagtatggcc
    22801 gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac caactttatc
    22861 tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt gaccgcccgc
    22921 tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg ttggcctgga
    22981 gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc cagggaaact
    23041 tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca ggtgccagtt
    23101 ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc tcagcagcgt ctgctttctg
    23161 atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg gcgttactgc
    23221 gccgtcgtag tggtgaaagc aaggaactgg agcgtattga aaccgaagcg caacgtctgg
    23281 acagcatgat caacgatctg ttggtgatgt cccgtaatca gcaaaaaaac gcgctggtta
    23341 gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag
    23401 ccgagcagat gggcaagtcg ttgacggtta acttcccgcc tgggccgtgg ccgctgtacg
    23461 gcaatccgaa cgcactggaa agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt
    23521 cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc attacggtgg
    23581 acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt ccgttctatc
    23641 ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg gcgattgttg
    23701 aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg ctgggcggtt
    23761 tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat ttgtaggcag
    23821 gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc
    23881 gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga aaaatcttcg
    23941 atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga taacccggcg
    24001 gcggagagta aacggtgata ttgatcgtca tcaaacggca tatgccatgc gatggcggca
    24061 gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc gtccgcagac
    24121 actttccctg gcgctatcac gctatacaac caccccacct tacctgtgtt ttgcatcaac
    24181 tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg
    24241 ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatataaac attactttcc
    24301 gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa ctccgcctgt
    24361 tccggaaatt cccgcgccca gtagagataa tgctcacgag gataatgaca cagcgctctg
    24421 tctggtccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc cagctccgtc
    24481 agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc ttcgggataa
    24541 gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct ctccacgaga
    24601 aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc
    24661 caggcaggaa ctcaatagca aaccggacga acgttagttc atccggctgt ggggattaag
    24721 gaagcaggtt tatttcaccg tacctaaaat ttcgattttc gctgctctgg ctttgacttt
    24781 acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc
    24841 taccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt ctgcaatcag
    24901 cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc
    24961 gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga taattactgc
    25021 cacacccaac aggatcccca gcaaaccagt ctgggcaatc acagtcaaat caatggtatt
    25081 gcccagcgcg aaggcaaaga atggaatcag cgtttgcacc gctttgctga aaaattctcg
    25141 taattcaggg tcaaggttcc ccagggcaaa gcccaccagg aacggtaata ctgcgccgac
    25201 gaaaacatgc ggttcaaacg aggcaatccc tgcagtgccc agaataatca tcgtcatgag
    25261 cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttcttttg tgccgtactg
    25321 ctgcatgatg gaagcgtaaa gtccgccatt ggtcatatcc atcgccgcca ccagcgccag
    25381 cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa tgcgcgaggc
    25441 aattgccgca accacccacg cgacggcaat ttttgttacc accagcgtac cggatttacg
    25501 caataccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc
    25561 cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc
    25621 ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca tcatcccccc
    25681 cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa
    25741 gggctgatgc gtcattcctc aatggagcat cagtgttgat ggaaacgaag atagacattc
    25801 atttaatcaa taaaagtgat caa
//
LOCUS       DAPPNJ010000051        25565 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000051 DAPPNJ010000000
VERSION     DAPPNJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.051"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            439..1578
                     /gene="wza"
                     /locus_tag="RPM73_001736"
     CDS             439..1578
                     /gene="wza"
                     /locus_tag="RPM73_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDX6520108.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1584..2027
                     /gene="wzb"
                     /locus_tag="RPM73_001737"
     CDS             1584..2027
                     /gene="wzb"
                     /locus_tag="RPM73_001737"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDX6520109.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2030..4192
                     /gene="wzc"
                     /locus_tag="RPM73_001738"
     CDS             2030..4192
                     /gene="wzc"
                     /locus_tag="RPM73_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDX6520110.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4285..5124
                     /gene="wcaA"
                     /locus_tag="RPM73_001739"
     CDS             4285..5124
                     /gene="wcaA"
                     /locus_tag="RPM73_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDX6520111.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQKYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5127..5615
                     /gene="wcaB"
                     /locus_tag="RPM73_001740"
     CDS             5127..5615
                     /gene="wcaB"
                     /locus_tag="RPM73_001740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDX6520112.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5612..6829
                     /gene="wcaC"
                     /locus_tag="RPM73_001741"
     CDS             5612..6829
                     /gene="wcaC"
                     /locus_tag="RPM73_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDX6520113.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFLEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6804..8021
                     /gene="wcaD"
                     /locus_tag="RPM73_001742"
     CDS             6804..8021
                     /gene="wcaD"
                     /locus_tag="RPM73_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDX6520114.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8032..8778
                     /gene="wcaE"
                     /locus_tag="RPM73_001743"
     CDS             8032..8778
                     /gene="wcaE"
                     /locus_tag="RPM73_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDX6520115.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPISGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            8794..9342
                     /gene="wcaF"
                     /locus_tag="RPM73_001744"
     CDS             8794..9342
                     /gene="wcaF"
                     /locus_tag="RPM73_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDX6520116.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9369..10490
                     /gene="gmd"
                     /locus_tag="RPM73_001745"
     CDS             9369..10490
                     /gene="gmd"
                     /locus_tag="RPM73_001745"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDX6520117.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLKEMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10493..11458
                     /gene="fcl"
                     /locus_tag="RPM73_001746"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10493..11458
                     /gene="fcl"
                     /locus_tag="RPM73_001746"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDX6520118.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11461..11940
                     /gene="gmm"
                     /locus_tag="RPM73_001747"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11461..11940
                     /gene="gmm"
                     /locus_tag="RPM73_001747"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDX6520119.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11937..13160
                     /gene="wcaI"
                     /locus_tag="RPM73_001748"
     CDS             11937..13160
                     /gene="wcaI"
                     /locus_tag="RPM73_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDX6520120.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13163..14599
                     /gene="cpsB"
                     /locus_tag="RPM73_001749"
     CDS             13163..14599
                     /gene="cpsB"
                     /locus_tag="RPM73_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDX6520121.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14704..16074
                     /gene="cpsG"
                     /locus_tag="RPM73_001750"
                     /gene_synonym="manB"
     CDS             14704..16074
                     /gene="cpsG"
                     /locus_tag="RPM73_001750"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDX6520122.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGLFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16129..17523
                     /gene="wcaJ"
                     /locus_tag="RPM73_001751"
     CDS             16129..17523
                     /gene="wcaJ"
                     /locus_tag="RPM73_001751"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDX6520123.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDLDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17525..19003
                     /gene="wzxC"
                     /locus_tag="RPM73_001752"
     CDS             17525..19003
                     /gene="wzxC"
                     /locus_tag="RPM73_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDX6520124.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19280..20560
                     /gene="wcaK"
                     /locus_tag="RPM73_001753"
     CDS             19280..20560
                     /gene="wcaK"
                     /locus_tag="RPM73_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDX6520125.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINSLNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEDGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEAK"
     gene            20557..21777
                     /gene="wcaL"
                     /locus_tag="RPM73_001754"
     CDS             20557..21777
                     /gene="wcaL"
                     /locus_tag="RPM73_001754"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDX6520126.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21788..23182
                     /gene="wcaM"
                     /locus_tag="RPM73_001755"
     CDS             21788..23182
                     /gene="wcaM"
                     /locus_tag="RPM73_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDX6520127.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQLIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGAALRMNFDMRKDVRGVFMAKRETLLSLANIHAVNETGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23340..24335
                     /locus_tag="RPM73_001756"
     CDS             23340..24335
                     /locus_tag="RPM73_001756"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HDX6520128.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24578..25471
                     /gene="galF"
                     /locus_tag="RPM73_001757"
     CDS             24578..25471
                     /gene="galF"
                     /locus_tag="RPM73_001757"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDX6520129.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAVLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6307 a         6361 c         6937 g         5960 t
ORIGIN      
        1 aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc cgaattgtta
       61 ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag agccaatatt
      121 caattaccat aatgtcacct tatattcgga agttaatttc cgcaataacc ctttctcatc
      181 aacgattgta tgcagagagg gtttgaaaaa ttaagctaaa tcagtgtatt ggtagctaaa
      241 aagccagggg cggtagcgtg tctggatgcc tgaaagacca gtctgaatta ttctgtcaat
      301 agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaatttgt attcaattaa
      361 ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata attactctgc
      421 caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt attggtgtca
      481 gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac gatgggcaaa
      541 gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa tgtttatccg
      601 ctgaccccgc gcctgattga ccaattacgc ccacgcccga atgtagcgcg ccccaatatg
      661 acgctggaaa gtgagatcgc gaattaccag tatcgcgtcg ggccgggcga cgttctgaat
      721 gtcaccgtct gggatcaccc ggaactcacc acgccagccg gtcagtaccg cagctccagc
      781 gacaccggca actgggtaca gcctgacggc actatgtttt acccgtatat cggcaaggtc
      841 cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg cttagcgacg
      901 tacatcgctg acccgcaggt ggatgttaat atcgccgcct tccgctcgca aaaggcctat
      961 atctcaggtc aggtgaataa atccggtcaa caggcgatca ccaacgtacc actgaccatt
     1021 ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg caacgtggtg
     1081 ctaacacaca atggtcgtga agaacgcatt tctttgcagg cgctgatgca aaacggcgac
     1141 ctcaatcaga accgactgct ttaccccggc gatattctct acgtgccgcg taatgatgat
     1201 ctgaaagtgt ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat ggactttagc
     1261 ggcatgaccc tgactgaagc cctgggcaat gccgaaggta tcgacatgac cacctccaac
     1321 gccagcggca tctttgtcat tcgtccgctg aaaggcgagg gcgggcgtaa cggcaagatt
     1381 gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtgatggc gacagaattc
     1441 cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtttcccg ctggaaccgt
     1501 ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga tacagccagc
     1561 gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt gtcggcaata
     1621 tttgccgttc cccgacggcg gaacgcttac tgcaacgtta tcacccggag ctgaaagtgg
     1681 agtccgctgg actcggcgcg ctggtcggta agggcgctga ccctactgct atcagcgtcg
     1741 ccgcagaaca tcaactgtct ctggaaggtc actgtgcccg gcaaatcagc cgccgcctgt
     1801 gtcgcaacta tgacctgatt ttgaccatgg aaaagcgcca tatcgaacgc ttatgcgaga
     1861 tggcaccgga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac gaatgtgaaa
     1921 tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca ttacttgaac
     1981 ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa tgacagaaaa
     2041 agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg gtcgcctggt
     2101 cggcaccgtc attgaagcgc gctggtgggt gattggcatc accgctgtat ttgccctctg
     2161 tgccgtggtt tacactttct tcgccacgcc gatttatagt gccgacgcac tggtacaaat
     2221 cgagcaaaac agcggcaatt cgttagtgca ggacattggc tctgcattag ccaataaacc
     2281 gccagcatcg gatgccgaga tccagttgat tcgttcgcgt ctggtgcttg gtaaaacggt
     2341 ggacgatcta gacctcgata ttgcggtgag caaaaacacg ttcccgattt tcggtgcggg
     2401 ctgggatcgc ctgatgggac gtcagaacga gacggtgaaa gtgaccacct ttaaccgccc
     2461 gaaagagatg gcggaccagg tgtttacgct taatgtgctg gatgacaaaa actacaccct
     2521 gagcagtgat ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga aaaaagaagg
     2581 cgtcacgctg atggttgaag ccattcacgc ccgcccgggc agtgagttta ccgtcaccaa
     2641 atactccacg ttggggatga tcaaccaact gcaaaacagc ctgacggtaa cggagaacgg
     2701 caaagacgca ggcgtactga gcctgactta taccggtgaa gatcgcgaac agatccgcga
     2761 cattcttaac agcatcgccc gtaactatca ggaacaaaat attgagcgca aatcggcgga
     2821 agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta gccgcctcga
     2881 tgttgccgaa aacaaactga atgccttccg tcaggataaa gattctgttg atctgccgct
     2941 ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga acgaactgac
     3001 ctttaaagag gcggaaatct ccaagctgta cactaaagtt cacccggcgt accgtacgct
     3061 gctggagaaa cgtcaggcgc tggaagaaga gaaagccaaa cttaacggtc gcgtaacggc
     3121 gatgccgaaa acccagcagg aaattgtccg tctgacccgc gatgtcgagt ctgggcagca
     3181 ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag ccagtaccgt
     3241 cggcgatgtg cgcattgttg acccggcaat cacccagcca ggtgtactga aaccgaagaa
     3301 aggactgatt atcctcggcg cgattatcct tggcctgatg ctctctatcg tgggtgtact
     3361 gctgcgctcg ttgtttaatc gcggcatcga aagcccacag gtgctggaag aacacggtat
     3421 tagcgtctat gccagcatcc cgctgtcgga atggcagaaa gcgcgcgata gcgtcaaaac
     3481 catcaaaggg gttaaacgct ataaacagag tcagctactg gcggtgggga atccaaccga
     3541 tctggcgatt gaagccatcc gtagtctgcg taccagtctg cacttcgcga tgatgcaggc
     3601 gcagaacaat gtgttgatga tgaccggggt tagcccgtca atcggtaaaa cctttgtctg
     3661 cgccaacctg gcggcggtga tcagccagac caataaacgg gtgttgttga tcgactgcga
     3721 tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat aacgttaatg gcctgtcgga
     3781 aattctgatt ggtcagggcg atattactac cgctgctaaa ccgacctcta ttgccaaatt
     3841 tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct gaactgttga tgagcgaacg
     3901 ctttgccgaa ctggtgaact gggcgagtaa aaactacgac ctcgtgttga ttgatacgcc
     3961 gccgattctg gcagtgaccg atgccgcaat tgtcgggcgt cacgctggaa ccacgttaat
     4021 ggtggcgcgt tatgcggtca acaccttgaa agaagtggaa accagtctga gccgctttga
     4081 gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc gcgccagcgc
     4141 gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat aacgacgcct
     4201 gcattcgcac cgccccgtag gccggataag gcgttcacgc cgcatccggc aagcaaacca
     4261 gctcataagc cgggagaaca acctatgaaa gacaatccgc tgatctcaat ctatatgccg
     4321 acatggaacc gtcaacaact ggcgattcgg gcgataaaat ctgtcctgcg ccaggactat
     4381 agcaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca actgcaaaag
     4441 tacgtcaccg ccctcaacga tccgcgtatc acttacatcc acaacgatat taacagcggg
     4501 gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatacat taccgggatc
     4561 gatgacgatg atgaatggac gcccaaccgc ctgagcgtct tcctcgccca taaacagcaa
     4621 ctggtcactc acgccttttt gtacgctaac gactacgttt gccagggcga agtctattcc
     4681 caaccagcaa gtttgccact gtatcccaaa tcgccgtatt cacgccgcct gttctataaa
     4741 cgcaatatca ttggcaatca ggtctttacc tgggcgtggc gtttcaaaga gtgtttgttc
     4801 gataccgaac tgaaagcggc acaggattac gacatcttcc tgcggatggt ggtggagtat
     4861 ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca cggtgaaatg
     4921 cagatcacct cgtcaccgaa aaaattctcc ggatacttcc acttttaccg caagcacaaa
     4981 gacaaattcg accgcgccag caaaaaatat cagctgttta ccctctacca gatccgcaat
     5041 aaacgtatga cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg caaacgtctg
     5101 gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgtgccaac agctggagtt
     5161 tacgcccgtg ctgcatggtt cttgcctatc gcgtcgctca tttttgttca gtgtggcgca
     5221 aaaagaacgt cctcaacaat ctgtgggcgg ccccgctgct ggtgctgtat cgcattatca
     5281 ccgaatgctt tttcggttat gaaatccagg cagccgcaac cattggccgc cgctttacta
     5341 tccatcacgg ttacgccgta gtgatcaata aaaacgtggt tgcgggggat gatttcacca
     5401 ttcgccacgg cgtcactatc ggcaatcggg gtgccgataa catggcatgt ccacacattg
     5461 gcaacggcgt cgaactcggt gccaacgtca ttattcttgg tgatatcacg cttggtaaca
     5521 acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac gcgctggtgg
     5581 tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ttgcaattta atgtgcgact
     5641 ggcggaaggc ggggcagcag gtgtggcgtt agatctccac cagcgtgcgc tgcaacaggg
     5701 gctggcgtca cattttgtct acggttacgg caaaggcggc aaagagagcg tcagccatca
     5761 gaactatccg caggtaatca aacatacgcc gcggatgacc gcgatggcga atattgccct
     5821 gtttcgtctg tttaatcgcg atctgtttgg caatttcaat gaattatacc gcaccattac
     5881 tcgcacaccg ggtccggtgg tcctgcattt tcatgtgctg cacagctact ggctaaatct
     5941 taagagcgtg gtgcgctttt gcgaaaaggt gaaaaaccac aaaccggatg tcactctggt
     6001 ctggacgctg cacgaccact ggagcgttac cgggcgctgc gcctttaccg acggttgtga
     6061 aggctggaaa acgggctgcc agaaatgccc gaccttaaat aactatccgc cggtgaagat
     6121 tgatcgggcg caccagctgg tggcgggcaa acgccagtta ttccttgaga tgctggcgct
     6181 gggctgtcag tttatttccc ccagccagca tgtggctgac gctttcaata gcctgtacgg
     6241 tccagggcgt tgccggatta tcaataacgg tattgatatg gcaaccgaag cgattctggc
     6301 ggatctgcct ccggtgcgcg aaacccaggg taagccgaaa atcgcggtgg tggcgcatga
     6361 cctgcgttac gacggcaaaa ctaaccagca actggtgcgt gagatgatgg cgctgggcga
     6421 caaaatcgaa ctgcatacct ttggtaagtt ctcgccgttc accgctggca acgtggttaa
     6481 tcacggcttt gaaactgaca agcgcaagtt gatgagcgcg ctcaatcaga tggatgcgct
     6541 ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgc tatcgattgg
     6601 tgtgccggtg atcgccaccc atagcgatgc ggctcgggaa gtgctgcaaa aatccggcgg
     6661 taaaactgtc agcgaagaag aggtgctgca actggtccag ttaagcaaac cggaaatcgc
     6721 gcaggcgata tttggtacca cgctggctga gttcagccaa cgcagccgcg ccgcctacag
     6781 tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc tacctgctgc
     6841 tgccgctgat ctacctgctg gttaacgtca aaatcgccca gcttggcgaa agtttcccca
     6901 tcaccatcgt cactttttta cctgtcttgt tactgctgtt tttagaacgc atcagcgtta
     6961 aaaaattgat gattgcctta gggattggcg cggggctcac ggcgttcaac tatctgtttg
     7021 gtcagtcgtt ggatgccagt aaatacgtca cttcaaccat gctgtttgtc tatattgtga
     7081 tcattattgg catggtgtgg agtattcgct ttaaaacgat ttcgccacac aaccatcgca
     7141 agatattacg tttcttttat ctggtggtcg ggctggtagt ggcgctggcg gcggtggaga
     7201 tggcgcaaat tatcctcacc ggtggcagca gtattatgga gtcgatttcg aaatatctga
     7261 tttacagcaa cagctatgta ctgaatttca tcaaattcgg cggcaagcgc acgacagcac
     7321 tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc agcatcaaac
     7381 agtttggtat caaaacgcct aaaaccgatg ctatgattct cgcagggata atattatccg
     7441 gatcgttttc aggggttatg acctttatcc tgttttattt gctggagtgg gcatttcaat
     7501 atctgaataa agaggcgatt aagaaaaagt taccgttagc attgatttct ctggctgtat
     7561 tcctggttgg tgtggtaatt gcatttcctt atatttccac ccgtctgggc gatttaggta
     7621 cggaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc ggttattctt
     7681 tgacccatat tgacggtgta gtcagatttg gctcacttta tgaatatgtc gcatcattcg
     7741 gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta
     7801 tttatttttc ctggttcgcg gtgtttttat cactgtggta catggggaaa gtgataaaaa
     7861 tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat cttttcactc
     7921 cggtgtcgct gttttttacc ggttcgatat ttagcccgga atatgcattt ttaatcgtct
     7981 gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta agaataagaa catgttgctt
     8041 agcataatca ctgtcgcgtt tcgtaacctc gaagggatag tcaaaacaca tgcctcgctg
     8101 gcgcatctgg cgcaggcgga cgatatcagc ttcgaatgga ttgttgtcga tggcggttcc
     8161 aatgacggca cccgtgagta tctggaaaat ctcaatggta tctataacct acgctttgtc
     8221 agcgagccag ataacggtat ctacgacgcc atgaacaaag gcattgcgat ggcacaaggc
     8281 aagttcgcgt tgtttctcaa ctcgggcgat atttttcatc aggatgccgc aaattttgtc
     8341 cgtaagttaa aaatgcaaaa agataacgtg atgatcaccg gcgatgcgct gctggatttt
     8401 ggcgacgggc ataaaattaa acgtagcgcc aaaccgggct ggtatattta tcacagcctg
     8461 cccgccagtc atcaggcgat atttttcccg atatccggct tgaaaaaatg gcgttatgac
     8521 ctggaatata aagtttcttc cgattacgcg ctggcggcta aaatgtataa agccggttat
     8581 gcatttaaaa aactcaatgg cctggtgtct gaattttcca tgggtggggt atcaaccacc
     8641 aataatatgg aattgtgtgc tgacgccaaa aaagtccaac ggcaaatatt acatgtgcct
     8701 ggcttctggg cagaattatc ctggcattta cgccaacgta cgacaggtaa gactaaagcc
     8761 ttatataaca aagtctgaat ataaggaata ccaatgcaag atttaagcgg tttctcggtg
     8821 ccgaaagggt tccggggcgg caacgctatt aaagtgcaat tgtggtgggc agtacaggca
     8881 acattatttg cctggtcgcc acaagtattg tatcgctggc gggcattttt attacgttta
     8941 ttcggcgcaa aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat tacctatccg
     9001 tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg acgtcaattt atataccctc
     9061 ggtgaaataa ccattggcgc acattcggtg atatcgcaaa aaagttattt atgcaccggg
     9121 agccacgacc atgcaagtca acatttcacc attaacgcca cgcctattgt gattggcgag
     9181 aaatgctggc tggcgacaga tgtttttgtt gcccctggcg tcacgattgg cgacggcacc
     9241 gtcgtgggtg cacgaagcag tgtttttaaa tcgcttccgg caaatgtggt ttgccggggg
     9301 aatcccgcag tggtgatacg cgaacgcgtt gaaactgaat aaattcaaaa aatacagagg
     9361 aataagacat gtcaaaagtc gctctcatca ccggtgtaac cggacaagac ggttcttacc
     9421 tggcagagtt tctgctggaa aaaggttacg aggtgcatgg tattaagcgt cgtgcatcgt
     9481 cattcaacac cgagcgcgtg gatcacattt atcaggatcc gcacacctgc aacccgaaat
     9541 tccatctgca ttatggcgac ctgagtgata cctccaacct gacgcgcatt ttgcgtgaag
     9601 tgcagccgga cgaagtgtac aacctgggcg caatgagcca cgttgcggtt tcttttgagt
     9661 caccggaata taccgcagac gttgatgcga tgggtacgct gcgcttgctg gaggcgatcc
     9721 gcttcctcgg tctggaaaag aaaacccgtt tctatcaggc ttccacttct gaactgtacg
     9781 gtctggtgca ggaaattccg cagaaagaaa ccacgccgtt ctacccacgc tctccgtatg
     9841 cggtcgccaa actgtatgcc tactggatca ccgttaacta ccgcgaatcc tacggcatgt
     9901 acgcctgtaa cggtattctc ttcaaccatg aatccccgcg ccgcggcgaa accttcgtta
     9961 cccgcaaaat cacccgcgca atcgccaata tcgcccaggg gctggagtcg tgcctgtacc
    10021 tcggcaatat ggattccctg cgtgactggg gccatgccaa agactacgta aaaatgcagt
    10081 ggatgatgct gcaacaggaa caaccggaag atttcgttat cgcgaccggc gttcagtact
    10141 ccgtacgtca gttcgtggaa atggcggcag cacagctggg catcaaactg cgctttgaag
    10201 gcacgggcgt tgaagagaag ggcattgtgg tttccgtcac cgggcatgac gcgccgggcg
    10261 ttaaaccggg cgatgtgatt atcgccgttg acccgcgtta cttccgtccg gcggaagttg
    10321 aaacgctgct cggcgacccg accaaagcgc acgaaaaact gggctggaaa ccggaaatca
    10381 ccctcaaaga gatggtgtct gaaatggtgg ctaatgacct cgaagcggcg aaaaaacact
    10441 ctctgctgaa atctcacggc tacgacgtgg cgatcgcgct ggagtcataa gcatgagtaa
    10501 acaacgcatt tttatcgctg gtcatcgcgg gatggtcggt tctgccatca agcggcagct
    10561 cgaacagcgc ggtgatgtgg aactggtatt acgcacccgc gacgagctga acctgctgga
    10621 cagccgcgcg gtgcatgatt tctttgccag cgaaagcatt gaccaggtct atctggcggc
    10681 ggcgaaagtg ggcggcattg ttgccaacaa cacctatccg gcggatttca tctaccagaa
    10741 catgatgatt gagagcaaca tcattcacgc cgcgcatcag aacgacgtga acaaactgct
    10801 gtttctcgga tcgtcctgca tctacccgaa actggcaaaa cagccgatgg cagaaagcga
    10861 gctgttgcag ggtacgctgg agccgaccaa cgagccttat gccattgcca aaatcgccgg
    10921 gatcaaactg tgcgaatctt acaaccgcca gtatggacgc gattaccgct ctgtcatgcc
    10981 gaccaacctg tacgggccgc atgacaactt ccacccgagt aattcgcatg tgatcccagc
    11041 attgctgcgt cgcttccacg aggcgacggc acagaatgcg ccggacgtgg tggtgtgggg
    11101 cagcggcaca ccgatgcgcg aatttctgca cgtcgatgat atggcggcgg cgagcattca
    11161 tgtcatggag ctggcgcacg aagtctggct ggagaacacc cagccgatgc tgtcgcacat
    11221 taacgttggt acgggcgttg actgcactat ccgcgagctg gcgcaaacca tcgccaaagt
    11281 ggtgggttac aaaggccggg tggtttttga tgccagtaaa ccggatggta cgccgcgcaa
    11341 actgctggat gtgacgcgcc tgcatcagct tggctggtat cacgaaatct cactggaagc
    11401 ggggcttgcc agcacttacc agtggttcct tgagaatcaa gaccgctttc gggggtaatg
    11461 atgtttttac gtcaggaaga ctttgccact gtagtgcgct ccaccccgct tgtctctctc
    11521 gactttattg tcgagaacag tcgcggcgag tttctgcttg gcaaaagaac caaccgcccg
    11581 gcgcagggtt actggtttgt gccgggaggg cgcgtgcaga aagacgaaac gctggaagcc
    11641 gcatttgagc ggctgacgat ggcggaactg gggctgcgtc tgccgataac agcaggccag
    11701 ttttacggtg tctggcagca cttttatgac gataacttct ccggcacgga tttcaccact
    11761 cactatgtgg tgctcggttt tcgcttcaga gtagcggaag aagaactgtt actgccggat
    11821 gagcagcatg acgattaccg ctggctgacg ccggacgcac tgctcgccag tgacaatgtt
    11881 cacgctaaca gccgcgccta ttttctcgct gagaagcgtg ccggagtacc cggattatga
    11941 aaatactggt ctacggcatt aactactcgc cggagttaac cggcatcggc aaatacaccg
    12001 gagaaatggt ggaatggctg gcggcgcaag gtcatgaggt gcgggttatt accgcaccgc
    12061 cttactaccc gcagtggcag gtgggcgaga actattccgc ctggcgctac aaacgagaag
    12121 agggggccgc cacggtgtgg cgctgcccgc tgtacgtgcc aaaacagccg agcaccctga
    12181 aacgcctgtt gcatctcggc agttttgccg tcagcagttt tttcccgttg atggcgcaac
    12241 gtcgctggaa gccggatcgc attatcggcg tggtgccaac gctgttttgc acgccgggaa
    12301 tgcgcctgct ggcgaaactc tctggtgcgc gtaccgtgct gcatattcag gattacgaag
    12361 tggacgccat gctggggctg ggccttgccg gaaaaggcaa aggcggcaaa gtggcacagc
    12421 tggcaacggc gttcgaacgt agcggactgc ataacgtcga taacgtctcc acgatttcgc
    12481 gttcgatgat gaataaagcc atcgaaaaag gcgtggcggc ggataacatc attttcttcc
    12541 ccaactggtc ggaaatcgcc cgttttcagc atgttgcaga cgccgatgtt gatgcccttc
    12601 gtaaccagct tggcctgccg gataacaaaa aaatcattct ttactccggc aatattggtg
    12661 aaaagcaggg actggaaaac gttattgaag ctgccgatcg cctgcgcgat gaaccgctga
    12721 tttttgccat tgtcgggcag ggcggcggca aagcgcggct ggaaaaaatg gcgcagcagc
    12781 gtggactgcg caacatgcaa tttttcccgc tgcaatcgta tgacgcttta cccgcactgc
    12841 tgaagatggg cgattgccat ctggtggtgc aaaaacgcgg cgcggcagat gccgtattgc
    12901 cgtcgaaact gaccaatatt ctggcagtag gcggtaacgc ggtgattacc gcagaggccc
    12961 acacagaact gggacagctt tgcgaaacct ttccgggcat tgcggtttgc gtggaaccgg
    13021 aatcggtcga ggcgctggtg gcggggattc gtcaggcgct cctgctgccc aaacacaaca
    13081 cggtggcacg tgaatatgcc gagcgcacgc tcgataaaga gaacgtgtta cgtcaattta
    13141 taaatgatat tcggggataa ttatggcgca gtcgaaactc tatccagttg tgatggcagg
    13201 tggctccggt agccgcttat ggccgctttc ccgcgtactt taccccaagc agtttttatg
    13261 cctgaaaggc gatctcacca tgctgcaaac caccatctgc cgcctgaacg gtgtggagtg
    13321 cgaaagcccg gtggtgattt gcaatgagca gcaccgcttt attgtcgcgg aacagctgcg
    13381 tcaactgaac aaactcaccg agaacattat tctcgaaccg gcagggcgta acactgcacc
    13441 tgccattgca ctggcggcgc tggcggcaaa acgtcatagc ccggagagcg acccgttaat
    13501 gctggtgctg gcggcggatc atgtgattgc cgatgaagac gcgttccgtg ccgccgtgcg
    13561 taatgccatg ccgtatgccg aagcgggcaa gctggtgacc ttcggcattg tgccggatct
    13621 acctgaaacc ggatatggct atattcgtcg cggtgaagtg tcggcgggtg agcaggatac
    13681 ggtggccttt gaagtggcgc agtttgtcga aaaaccgaat ctggaaaccg cccaggccta
    13741 tgtggcaagc ggcgaatatt actggaacag cggcatgttc ctgttccgcg ccggacgcta
    13801 tctcgaagaa ctgaaaaaat atcgcccgga tattctcgac gcctgtgaac aagcgatgag
    13861 cgccgtcgat ccggatctcg attttattcg cgtggatgaa gaagcgtttc tggcctgccc
    13921 ggaagagtcg gtggattacg cggtcatgga acgtacggca gatgccgttg tggtgccgat
    13981 ggatgcgggc tggagcgatg tcggttcctg gtcttcatta tgggagatca gcgcccacac
    14041 cgccgagggc aacgtttgcc acggcgatgt gattaatcac aaaactgaaa acagctatgt
    14101 gtacgccgaa tctggcctgg tcaccaccgt cggggtgaaa gatttggtgg tagtgcagac
    14161 caaagatgcg gtgctgattg ccgaccgtaa cgcggtgcag gatgtgaaaa aagtggtcga
    14221 gcagatcaaa gccgatggtc gccatgagca tagggtgcat cgcgaagtgt atcgcccgtg
    14281 gggcaaatat gactctatcg acgcaggtga ccgctaccag gtgaaacgca tcaccgtgaa
    14341 accgggcgaa ggcttgtcgg tacagatgca ccatcaccgc gcggaacact gggtggtggt
    14401 cgcgggaacg gcaaaagtca ccattgacgg tgatatcaaa ctgcttggtg aaaacgagtc
    14461 catttatatt ccgctggggg cgacgcactg cctggaaaac ccggggaaaa ttccgctcga
    14521 tttaattgaa gtgcgctccg gctcttatct cgaagaggat gatgtggtgc gcttcgcgga
    14581 tcgctacgga cgggtgtaaa cgtcgcatca ggcaatgaat gcgaaaccgc ggtgtaaata
    14641 acgacaaaaa caaaattggc cgtttcggtc agggccaact attgcctgaa aaagggtaac
    14701 gacatgaaaa aattaacctg ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa
    14761 ctgaatgaag atattgcctg gcgcattggt cgcgcttatg gcgaatttct caaaccgaaa
    14821 accattgtgt taggcggtga tgtccgcctc accagcgaaa ccttaaaact ggcgctggcg
    14881 aaaggtttac aggatgcggg cgtcgatgtg ctggatattg gcatgtccgg caccgaagag
    14941 atctatttcg ccacgttcca tctcggtgtg gatggcggca ttgaagttac cgccagccat
    15001 aacccgatgg attacaacgg catgaaactg gtgcgcgaag gggctcgccc gatcagcggt
    15061 gataccggac tgcgcgatgt ccagcgcctg gctgaagcca acgactttcc tcccgtcgat
    15121 gaaaccaaac gcggtcgcta tcagcaaatc aacctgcgtg acgcttacgt tgatcacctg
    15181 ttcggttata tcaatgtcaa aaatctcacg ccgctcaagc tggtgatcaa ctccgggaat
    15241 ggcgcagcgg gtccggtggt ggacgccatt gaagcccgct ttaaagccct cggcgcgccc
    15301 gtggaattaa tcaaagtaca caacacaccg gacggcaatt tccccaacgg tattcctaac
    15361 ccgctgctac cggaatgtcg cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat
    15421 atgggcattg cctttgatgg cgattttgac cgctgtttcc tgtttgacga aaaagggctg
    15481 tttatcgagg gctattacat tgtcggcctg ctggcagaag cattcctcga aaaaaatccc
    15541 ggcgcgaaga tcatccacga tccacgtctc tcctggaaca ccgttgatgt ggtgactgcc
    15601 gcaggtggca cgccggtaat gtcgaaaacc ggacacgcct ttattaaaga acgtatgcgc
    15661 aaggaggacg ccatctacgg tggcgaaatg agcgctcacc attacttccg tgatttcgct
    15721 tactgcgaca gcggcatgat cccgtggcta ctggtagccg aactggtgtg cctgaaagag
    15781 aaaacgctgg gcgaactggt acgcgaccgg atggtggcgt ttccggcaag cggtgagatc
    15841 aacagcaaac tggcgcaacc cgctgaggcg attaaccgcg tggaacagca ttttagccgt
    15901 gaggcgctgg cggtggatcg taccgatggc atcagtatga cctttgccga ctggcgtttt
    15961 aacctgcgct cctccaacac cgaaccggtg gtgcggttga atgtggaatc gcgcggtgat
    16021 gtgccgctga tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga gtaatgtcgg
    16081 atcttccctt accccactgc gggtaagggg ctaataacag gaacaacgat gacaaatcta
    16141 aaaaagcgcg agcgagcgaa aaccaatgca tcgttaatct ctatggtgca acgcttttca
    16201 gatatcacca tcatgtttgc cgggctatgg ctggtttgcg aagtgagcgg actgtcattc
    16261 ctctacatgc acctgttggt ggcgctgatt acgctggtgg tattccagat gctgggtggc
    16321 atcaccgatt tttatcgctc atggcgcggt gttcgggcag cgacagaatt tgccctgttg
    16381 ctacaaaact ggaccttaag cgtgattttc agcgccggac tggtggcgtt caacaatgat
    16441 ttagacacgc aactgaaaat ctggctggcg tggtatgggc tgaccagcat cggactggtg
    16501 gtttgccgtt catgtattcg cattggggcg ggctggttgc gtaatcatgg ctataacaag
    16561 cgcatggtcg cggtggcggg ggatttagcc gccggacaaa tgctgatgga gagcttccgt
    16621 aatcagccgt ggttagggtt tgaagtggtg ggggtttacc acgacccgaa accgggcggc
    16681 gtttctaacg actgggcggg caatctgcaa cagctggtcg aggatgcgaa agcgggcaag
    16741 attcataacg tctatatcgc gatgcaaatg tgcgacggcg cacgcgtgaa aaaactggtc
    16801 catcaactgg cggacaccac ctgttcggtg ttgctgatcc ccgatgtctt taccttcaac
    16861 attctccatt cacgcctcga agagatgaac ggcgttccgg tggtgccgct gtacgatacg
    16921 ccgctttccg gggttaaccg cctgctaaaa cgtgcggaag atattgtgct ggcgacgctt
    16981 attctgctgc tgatctcccc ggtgctgtgc tgtattgcgc tggcggtaaa actcagttca
    17041 ccaggaccgg ttattttccg ccagactcgc tatggcatgg atggcaagcc gatcaaagtg
    17101 tggaagttcc gttcgatgaa agtgatggag aacgacaaag tggtgaccca ggcgacgcag
    17161 aacgatccgc gcgtcactaa agtggggaac tttctgcgcc gcacctcgct ggatgaactg
    17221 ccgcagttta tcaatgtgct gaccgggggg atgtcgattg tggggccacg tccgcacgca
    17281 gtagcgcata acgaacagta tcgacagctc attgaaggct acatgctgcg ccataaggtg
    17341 aaaccgggca ttaccggctg ggcgcagatt aatggctggc gcggcgaaac cgacacgctg
    17401 gagaaaatgg aaaaacgcgt cgagttcgac cttgagtaca tccgcgaatg gagcgtctgg
    17461 ttcgatatca aaatcgtttt cctgacggta ttcaaaggct tcgttaacaa agcggcatat
    17521 tgatatgagc ttacgtgaaa aaaccatcag cggcgcgaag tggtcggcga ttgctacggt
    17581 gatcatcatc ggcctcgggc tggtacagat gaccgtgctg gcgcggatta tcgacaacca
    17641 ccagttcggt ttgcttaccg tgtcgctggt gatcatcgcg ctggcagata cgctttctga
    17701 cttcggtatt gctaactcga ttattcagcg aaaagaaatc agccacctgg aactcaccac
    17761 gctgtactgg ctgaacgtcg ggctggggct tgtggtgtgc gtggcggtgt ttttgttgag
    17821 tgatgccatt ggtgacgtgc tgaataaccc ggacctggca ccgttgatta aaacattgtc
    17881 gctggcgttt gtggtgatcc cccacgggca gcagttccgc gcgttgatgc aaaaggaact
    17941 ggagttcaac aaaatcggca tgatcgaaac cagcgcggtg ctggcgggct ttacttttac
    18001 ggtggttagc gcccatttct ggccgctggc gatgaccgcg atcctcggtt atctggtcaa
    18061 tagtgcggtg agaacgctgc tgtttggcta ctttggtcga aaaatttatc gccccggtct
    18121 gcatttctcg ctggcgtctg tggcaccaaa cttacgtttt ggtgcctggc tgacggcgga
    18181 cagcatcatc aactatctca ataccaacct ttcaacgctg gtgctggcgc gtattctcgg
    18241 cgcgggcgtg gcagggggat acaacctggc gtacaacgtg gccgttgtgc cgccgatgaa
    18301 gctgaaccca atcatcaccc gtgtgttgtt tccggcgttt gccaaaattc aggacgatac
    18361 cgaaaagctg cgcgtcaact tctacaagct gctgtcggtg gtggggatta tcaactttcc
    18421 ggcgctgctc gggctgatgg tggtgtcaaa taactttgta ccgctggtat ttggcgaaaa
    18481 gtggaacagc attattccgg tgctgcaatt gctgtgtgtg gtggggctac tgcgctccgt
    18541 gggtaacccg attggttcgc tgctgatggc gaaagcgcgg gtcgatatca gctttaaatt
    18601 caacgtattc aaaacatttc tgtttattcc ggcgattgtt attggtgggc agatggcggg
    18661 cgcaatcggc gtcacgctcg gtttcctgct ggtgcagatt atcaacacca ttctgagtta
    18721 cttcgtgatg attaaaccgg ttcttggttc cagttatcgc cagtacatcc tgagtttgtg
    18781 gctgccgttt tatctctcgt tgccgacgct ggtggtcagt tatgcgctgg gcattgtgct
    18841 gaaagggcaa ctggcgctgg ggatgctgct ggcggtgcaa atagccgcgg gcgtgctggc
    18901 gtttgtggtg atgattgtgc tgtcgcgcca tccgctggtg gtggaagtga agcgtcagtt
    18961 ttgtcgcagc gaaaaaatga aaatgctttt acgggcgggg tgagttgttg ttttcggttc
    19021 cgacaacggt tgccgggtgc agcgtaaaac gccttatcca tcctacggtt ctgtgctcgg
    19081 gtcttgtagg cctgataaga cgcgtcagcg tcgcatcagg cactgaatgc cggatgcggc
    19141 gtaaaacgcc ttatccatcc tacggttctg tgctcgggtc ttgtaggcct gataagacgc
    19201 gtcagcgtcg catcaggcaa aaagcggcaa cgaacaaaac aaacgcagcc tcacctggca
    19261 tcaaatttat gaggaaaaaa tgaaattact tattctgggc aaccacactt gcggcaaccg
    19321 cggcgacagc gccatcctgc gcggcttact tgatgccatc aacagcctca atccacacgc
    19381 cgaagtggat gtgatgagcc gctatccggt cagttcttcc tggctgctca accgcccggt
    19441 aatgggcgat ccgctgttcc tgcaaatgaa acaacacaac agcgcggcgg gcgttgtcgg
    19501 gcgcgttaaa aaagtcctcc gtcgccgcta ccagcatcag gtattgctct cacgcgtcac
    19561 cgacactggc aagctgcgca atatcgccat cgcccaggga ttcaccgact tcgtgcgcct
    19621 gctgtcaggt tacgacgcca ttatccaggt cggcggatcg ttttttgtcg atctctatgg
    19681 cgtgccgcag tttgaacatg cactttgcac tttcatggcg aaaaagccgc tgtttatgat
    19741 tggtcacagc gtcggtccct tccaggatga gcaatttaac caactggcga attacgtttt
    19801 tggtcactgt gacgcgctga tcctacgcga atcggtcagt ctcgaactga tgaaacgcag
    19861 caatatcacc actgcaaaag tggaagatgg cgtcgatacc gcgtggctgg tcgatcacca
    19921 cacagaagat ttcaccgcca gctatgccgt ccaacactgg ctggacgttg ccgcacaaca
    19981 gaaaacggtg gcaattaccc tgcgcgaact ggcaccgttc gacaaacgtc tcggcaccac
    20041 tcaacaagcg tatgaaaaag cctttgccgg ggtggtcaat cgcattctcg acgaagggta
    20101 tcaggtgatt gcgctatcca cctgtacggg catcgacagc tataacaaag acgaccgcat
    20161 ggtggcgctc aacctacgcc agcacatcag cgatcctgcc cgttatcacg tagtgatgga
    20221 tgaactcaac gatctggaaa tgggcaaaat tctgggggcc tgtgaactca ccgtcggtac
    20281 gcgcctgcac tccgccatta tctcaatgaa ctttgctact ccggcgattg ccatcaacta
    20341 tgaacataaa tccgccggga ttatgcagca gttgggacta ccggagatgg cgattgatat
    20401 ccgtcattta ctggacggca gcctgcaagc gatggttgcg gataccttag gccagcttcc
    20461 ggcgctgaac gcacgactta gcgaagccgt cagtcgtgag cgtcagacag gaatgcagat
    20521 ggtgcagtcc gtactggaac gcattgggga ggcgaaatga aggttggctt ctttttactg
    20581 aaatttccgc tgtcgtcgga aaccttcgtc ctcaaccaaa ttaccgcgtt tattgatatg
    20641 ggctttgagg tggagattgt cgcgctgcaa aaaggcgaca cacaaaacac ccacgcggca
    20701 tggacgaaat acaaccttgc cgccagaacc cgatggttac aggacgaacc tacgggcaaa
    20761 gtggcgaagc tgcgccaccg cgccagtcag accttgcgcg gcattcatcg taaaaatacc
    20821 tggcaggcgc tcaacctcaa acgctatggt gccgagtcgc ggaacctgat tttgtctgcc
    20881 atttgcggcc aggtcgcaac accgtttcgc gccgatgtgt tcatcgctca ttttggcccg
    20941 gcgggggtaa ccgcagcaaa actacgcgaa ctgggtgtca ttcgcggcaa aattgccact
    21001 attttccacg gcattgatat ttccagtcgg gaagtgctca accattacac tcccgaatat
    21061 cagcaactgt ttcgccgtgg cgacctgatg ttaccgataa gcgatttgtg ggccggaagg
    21121 ctgcaaaaaa tgggctgccc cagagaaaaa atcgccgtat cgcgtatggg cgtggacatg
    21181 acgcgcttta gcccgcgtcc ggtgaaagcg cccgcaacgc cgctggaaat catttccgtc
    21241 gcacgcttaa ccgaaaaaaa aggcctgcat gtggcgattg aagcctgccg tcagttgaaa
    21301 gagcagggcg tggcatttcg ctatcgcatc ctcggcattg gcccgtggga acgacgcttg
    21361 cgcacgctca tcgaacaata tcaactggaa gatgtggtgg agatgccggg ctttaaaccg
    21421 agccatgaag tgaaggcgat gctcgacgac gcggatgtct tcctcctgcc atcggtaacg
    21481 ggcgcggacg gtgatatgga aggtattccg gtggcgctaa tggaagcgat ggcggtcggc
    21541 attccggttg tttctactct acatagcgga ataccggaac tggttgaggc cgataaatcc
    21601 ggctggctgg tgcctgagaa cgatgctcgc gcactggcgc aacgactggc ggcgtttagc
    21661 caactggaca ccgacgaact ggctccggtc gtcaaacgtg cgcgcgaaaa agtcgaacac
    21721 gattttaacc agcaggtgat taaccgagaa ctcgccagct tgttgcaggc tttatagagg
    21781 agaacgtatg ccatttaaaa aactctcccg acgcaccttc ctgacggcaa gctccgcgct
    21841 tgccttcctc catacccctt tcgctcgcgc acttcccgcc cgacaaagcg ttaacattaa
    21901 cgactacaac cctcacgact ggatcgcctc atttaaacaa gccttcggcg aagggcaaac
    21961 ggtcgtcgtg cctgccggat tggtttgcga caatatcaac accggcatct tcattcctgc
    22021 aggtaaaacg ttacacatcc ttggaagcct gcgcggcaac ggcagagggc gatttgtctt
    22081 acaggacggc agccaggtga caggggggga gggcggcagt atgcataaca tcaccctgga
    22141 tgtgcgcggc tctgactgca ccatcaaagg gctgactatg agcggctttg gtccggtaac
    22201 gcagatttat atcggcggca aaaacaaacg ggtcatgcgc aacctgacca tcgataacct
    22261 gaccgttagc catgctaatt acgccatctt acgccaggga tttcataacc agcttatcgg
    22321 tgccaacatc accaactgta agttcagcga cttacaaggc gacgccattg aatggaacgt
    22381 ggcgattaac gaccgtgata ttttgatatc tgaccatgtc atcgagcgca tcaactgtac
    22441 caacggcaaa atcaactggg gtatcggcat aggccttgcg ggaagtacct acgataataa
    22501 ctacccggaa gaccaggcag tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg
    22561 tcggcagttg atccatgttg aaaatggtaa acattttgtt attcgtaata tcaaagcccg
    22621 caatatcacg ccggatttca gtaagaaagc aggtattgat aacgccacag tcgctattta
    22681 cggttgtgac aatttcgtga ttgataatat tgaaatgatt aatagcgccg ggatgttaat
    22741 cggctatggg gtaattaaag gcaaatatct ctcgataccg caaaatttcc gagtgaataa
    22801 tattcaactg gataacaccc atcttgctta taaattgcgc ggcatccaaa tctccgccgg
    22861 gaatgctgcc tcctttgtgg cactgactaa cattgagatg aagcgtgcct cgctggagct
    22921 tcacaataaa ccgcaacatc tttttatgcg taatatcaaa gtgatgcagg aatcctcagt
    22981 tggagccgca ttgcgcatga acttcgacat gcgcaaagac gttcgcggcg tctttatggc
    23041 aaagcgtgag acgctgcttt cacttgcgaa tattcatgca gtgaatgaaa cggggcaaag
    23101 ctccgtcgat atcgacagga ttaatcacca tattgttaat gtggaaaaga ttaactttag
    23161 attgccggaa cggagggagt agatttgcga ccattcataa acttactgga tataatgttc
    23221 taaaagatta taatcctcac actaactgtt aaagctgggg ctgggcagca ttgactactc
    23281 agggaaggta ctcatcttta tcttgtgttt cttacgtaac aaccagacta aagtgattta
    23341 tgaacgataa cgttttgctc ataggagctt ccggattcgt aggaacccga ctacttgaaa
    23401 cggcaattgc tgactttaat atcaagaacc tggacaaaca gcagagccac ttttatccag
    23461 aaatcacaca gattggcgat gttcgtgatc aacaggcact cgaccaggcg ttagccggtt
    23521 ttgacactgt tgtactactg gcagcggaac accgcgatga cgtcagccct acttctctct
    23581 attatgatgt caacgttcag ggtacccgca atgtgctggc ggccatggaa aaaaatggcg
    23641 ttaaaaatat catctttacc agttccgttg ctgtttatgg tttgaacaaa cacaaccctg
    23701 acgaaaacca tccacacgac cctttcaacc actacggcaa aagtaagtgg caggcagagg
    23761 aagtgctgcg tgaatggtat aacaaagcac caacagaacg ttcattaacc atcatccgtc
    23821 ctaccgttat cttcggtgaa cgcaaccgcg gtaacgtcta taacttgctg aaacagatcg
    23881 ctggcggcaa gtttatgatg gtgggcgcag ggactaacta taagtccatg gcttatgttg
    23941 gaaacattgt tgagtttatc aagtacaaac tgaagaatgt tgccgcaggt tatgaggttt
    24001 ataattacgt tgataagcca gatctgaaca tgaaccagtt ggttgctgaa gttgaacaaa
    24061 gtctgaacaa aaagatccct tctatgcact tgccttaccc actaggaatg ttggggggat
    24121 attgttttga tatcctgagc aaaattacgg gcaaaaaata cgctgtcagc tctgtgcgcg
    24181 tgaaaaagtt ctgcgcaaca acacagtttg acgcaacgaa agtgcattct tcaggttttg
    24241 tggcaccgta tacgctgtcg caaggtctgg atcgaactct gcagtatgaa ttcgtccatg
    24301 ccaaaaaaga cgacataacg tttgtttctg agtaatgcag acgagtcaga gaaaaaagga
    24361 ccaggttgga ctacgattat tcggaaagag gtactgatta aattatcagt atgcggaatt
    24421 ccgagtaatc cgagtaaccc agatgctgat ggtgtgtagt gttatcctaa ggtttaagta
    24481 aagttatgcg gaactaagct ttggtatata gccataacgt ttggctaacc tatggcaata
    24541 atttacgcac tatcagccac ttagaggtag gaaaagaatg acgaatttaa aagcagttat
    24601 accggtagca ggtcttggga tgcatatgtt gcctgccact aaggcgattc ccaaagagat
    24661 gctaccgatc gtcgacaagc caatgattca gtacattgtt gacgagattg tggctgcagg
    24721 gatcaaagaa atcctcctgg taactcacgc gtccaagaac gcggtcgaaa accacttcga
    24781 cacctcatat gaattagaat ctctccttga gcagcgcgtg aagcgtcaac tactggcgga
    24841 agtgcaatct atctgtccgc cgggcgtgac cattatgaac gtgcgtcagg gtgaaccttt
    24901 aggtttaggc cactccattt tatgtgcacg acccgccatt ggcgacaacc catttgtcgt
    24961 ggtgctgcca gacgttgtga tcgacgatgc cagcgccgac ccgctgcgct acaaccttgc
    25021 agccatgatt gcgcgcttca acgaaacggg acgcagccag gtactggcaa aacgtatgcc
    25081 gggtgacctc tctgaatact ccgtcatcca gaccaaagaa ccgctggacc gtgaaggcaa
    25141 agtcagccgc attgttgaat ttatcgaaaa accggatcag ccgcagacgc tggactcaga
    25201 catcatggcc gtaggtcgtt atgtgctttc tgccgatatt tggccggaac ttgaacgcac
    25261 gcagccaggg gcatggggac gtattcagct gacagatgcc atcgctgaac tggcgaaaaa
    25321 acaatctgtt gatgcagtgc tgatgactgg tgacagctac gactgcggta aaaaaatggg
    25381 ctatatgcag gcgtttgtga agtatggctt acgcaacctg aaagaagggg cgaagttccg
    25441 taaagggatt gagaagctgt taagcgaata atgaaaatct gaccggatgt aacggttgat
    25501 aagaaaatta taacggcggt gaagattcgt ggcgaaagta atttgttgcg agtgttcctg
    25561 ccatt
//
LOCUS       DAPPNJ010000052        25509 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000052 DAPPNJ010000000
VERSION     DAPPNJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.052"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            48..1550
                     /locus_tag="RPM73_001758"
     CDS             48..1550
                     /locus_tag="RPM73_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDX6520130.1"
                     /translation="MNIRKLFCPGNTPRIILFLFFFVVSVITTIACGYTEKNTTGNVL
                     LLFFLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSVTVLVFMVIFWQTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQHYKQLSQHGADSWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKSGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADTSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLINEDNETVMVFNGEKIVPADGLKSQPVILPDKGK"
     gene            complement(1815..3077)
                     /locus_tag="RPM73_001759"
     CDS             complement(1815..3077)
                     /locus_tag="RPM73_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDX6520131.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLISVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(3275..3350)
                     /locus_tag="RPM73_001760"
     tRNA            complement(3275..3350)
                     /locus_tag="RPM73_001760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3315..3317),aa:Phe,seq:gaa)
     gene            complement(3456..4163)
                     /locus_tag="RPM73_001761"
     CDS             complement(3456..4163)
                     /locus_tag="RPM73_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDX6520132.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPAMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            4561..6696
                     /gene="speC"
                     /locus_tag="RPM73_001762"
     CDS             4561..6696
                     /gene="speC"
                     /locus_tag="RPM73_001762"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX6520133.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6746..8002)
                     /gene="nupG"
                     /locus_tag="RPM73_001763"
     CDS             complement(6746..8002)
                     /gene="nupG"
                     /locus_tag="RPM73_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDX6520134.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(8204..9283)
                     /gene="mltC"
                     /locus_tag="RPM73_001764"
     CDS             complement(8204..9283)
                     /gene="mltC"
                     /locus_tag="RPM73_001764"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDX6520135.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMLPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(9348..9623)
                     /locus_tag="RPM73_001765"
     CDS             complement(9348..9623)
                     /locus_tag="RPM73_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDX6520136.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9651..10703)
                     /gene="mutY"
                     /locus_tag="RPM73_001766"
     CDS             complement(9651..10703)
                     /gene="mutY"
                     /locus_tag="RPM73_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDX6520137.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10864..11583
                     /gene="trmB"
                     /locus_tag="RPM73_001767"
     CDS             10864..11583
                     /gene="trmB"
                     /locus_tag="RPM73_001767"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDX6520138.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPLTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11583..11909
                     /locus_tag="RPM73_001768"
     CDS             11583..11909
                     /locus_tag="RPM73_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDX6520139.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            12093..12812
                     /locus_tag="RPM73_001769"
     CDS             12093..12812
                     /locus_tag="RPM73_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDX6520140.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12988..14034
                     /gene="ansB"
                     /locus_tag="RPM73_001770"
     CDS             12988..14034
                     /gene="ansB"
                     /locus_tag="RPM73_001770"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDX6520141.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            14151..15158
                     /locus_tag="RPM73_001771"
     CDS             14151..15158
                     /locus_tag="RPM73_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDX6520142.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(15313..16449)
                     /gene="hemW"
                     /locus_tag="RPM73_001772"
     CDS             complement(15313..16449)
                     /gene="hemW"
                     /locus_tag="RPM73_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDX6520143.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16442..17035)
                     /locus_tag="RPM73_001773"
     CDS             complement(16442..17035)
                     /locus_tag="RPM73_001773"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDX6520144.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(17043..17333)
                     /locus_tag="RPM73_001774"
     CDS             complement(17043..17333)
                     /locus_tag="RPM73_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDX6520145.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17330..17896)
                     /gene="yggT"
                     /locus_tag="RPM73_001775"
     CDS             complement(17330..17896)
                     /gene="yggT"
                     /locus_tag="RPM73_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDX6520146.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(17914..18618)
                     /gene="yggS"
                     /locus_tag="RPM73_001776"
     CDS             complement(17914..18618)
                     /gene="yggS"
                     /locus_tag="RPM73_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDX6520147.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            18636..19616
                     /locus_tag="RPM73_001777"
     CDS             18636..19616
                     /locus_tag="RPM73_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDX6520148.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(19792..20208)
                     /gene="ruvX"
                     /locus_tag="RPM73_001778"
     CDS             complement(19792..20208)
                     /gene="ruvX"
                     /locus_tag="RPM73_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDX6520149.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(20208..20771)
                     /locus_tag="RPM73_001779"
     CDS             complement(20208..20771)
                     /locus_tag="RPM73_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDX6520150.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(20880..21830)
                     /gene="gshB"
                     /locus_tag="RPM73_001780"
     CDS             complement(20880..21830)
                     /gene="gshB"
                     /locus_tag="RPM73_001780"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDX6520151.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(21843..22574)
                     /gene="rsmE"
                     /locus_tag="RPM73_001781"
     CDS             complement(21843..22574)
                     /gene="rsmE"
                     /locus_tag="RPM73_001781"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDX6520152.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(22654..23361)
                     /gene="endA"
                     /locus_tag="RPM73_001782"
     CDS             complement(22654..23361)
                     /gene="endA"
                     /locus_tag="RPM73_001782"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDX6520153.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(23456..23953)
                     /locus_tag="RPM73_001783"
     CDS             complement(23456..23953)
                     /locus_tag="RPM73_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDX6520154.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(24030..25424)
                     /gene="galP"
                     /locus_tag="RPM73_001784"
     CDS             complement(24030..25424)
                     /gene="galP"
                     /locus_tag="RPM73_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDX6520155.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6285 a         6390 c         6639 g         6195 t
ORIGIN      
        1 tttttattat cctgttctct tcataataat gttgtcgata tactccgatg aatataagaa
       61 aactgttttg tccgggcaat actccccgga ttatattgtt tttattcttt tttgttgttt
      121 ctgtcataac cacaattgca tgcggataca ctgagaagaa taccaccggg aatgtgttgc
      181 tgctgttctt tcttctgctt cttgcacaca gaaataccct cacatccatt acagcgctgt
      241 tatttctgtt ctgttgtgca ctgtatgcgc ctgcgggtat gacgtacggt aaaatcaaca
      301 acagttttat tgtcgcgttg ctgcagacca cggctgatga ggctgcagag tttaccggga
      361 tgattcctgt ttatcatttt ctggttagtg tcacggttct ggtattcatg gtgattttct
      421 ggcagacaca ccaccgcggt caccgtaact ggctggcact gctgctgttc gtattatgct
      481 cagtaaacag ctggccgttg cggatggtta aaggaacagt tgtggggaca actgacacat
      541 tgcgtgaaat gcagcattat aaacaactga gtcagcacgg ggctgacagc tggaaaatcc
      601 tgccgggtgt accgttgtat gacacgattg ttatcgttac tggtgagagt gtgcgcaggg
      661 attatatgtc ggtatatggt tatcccgtgc caaccacacc gtggctgaat acggctcccg
      721 gtttatttat tgacggctat acatcggcag cagccagtac cgtaccttcc ctgagccgga
      781 cactgattta tgactatgag cagaaccctg attccggcaa caacgtggtg gcgctggcag
      841 caaaatcagg atacagcaca tggtggatat ccaatcaggg aaaactggga gagcatgaca
      901 cacgcatctc tgttattgct tctgatgcgg agcataccgt tttcctcaag aaaggcagct
      961 tcgcttcccg taaaacggat gatatgttgt tgttacagga aacagaacgt gcgctggcgg
     1021 atacatcctc tccgaaggtg attttcctgc acatgattgg ctctcatccg aatccgtgtg
     1081 accgacttaa ctcctggccg aatcattacc tggagcagta tccccgaaag attgcctgtt
     1141 acctcgccag catcagtaaa ctggataact ttctcggtca gcttgatggt atccttcgcc
     1201 ggcattcccg tcatttcgcc atgctttact tttctgacca tgggctgtcg gtcagcgaca
     1261 gtgctaatcc tgttcatcat gatggtcatg tgcagggagg ctacagcgtt cccctgatta
     1321 ttaccgccag tgacatcacg tctcatcagt ccgtcagcag aaaaatcagt gcccgtcatt
     1381 tcgcaggcat ttttcagtgg ctgactggta ttcgtactga aaatataccg ccattcaacc
     1441 cgctgataaa tgaagataat gaaactgtta tggtttttaa cggagagaaa atagttcctg
     1501 cggacggcct taagtcacag cctgtgattc ttccggacaa gggaaaataa gagtaatgtt
     1561 gagcaccatg ttctgtacga tgaattttcc actgtttata tcgattattt tgctaataac
     1621 cagagttgag cacccgaaaa tacctgaaga ggaattaccc gcgaccagga ggttcagata
     1681 tccctgatgt cggctataaa aattttgatt acatgacgta atggaagtgg ttctgtcaat
     1741 tgccttgttt attcattaat ctcaaagaaa aaggtattga aaataattcc cgggctggca
     1801 gcccgggatg attactattt taatggattg ttaatctttg catattcaaa cgggctgata
     1861 cattgttctc tgttcgcatc cagaaaatct gcccaccact gtagcatcag tcgccgttct
     1921 tccagatgtt ctgctttatg aatgtaagca gcacgtacac cgttacgttc ctgatggctc
     1981 atctgacgtt caactgcatc gcgggaccac aaacctgatt ctattaacgc actacatgcc
     2041 atcgcccgga atccatgacc acagacatcc tgggttgtgt cataccccat aaccctcagg
     2101 gctttattta cggtgttttc actgattggt ttacgtgggt catgtgctcc cgtaaagatc
     2161 agaccatttt caccagccca tgtctgaagt tctgccagta tggctacaac ctgtttgctg
     2221 agtggcacaa gatgtggtgt tcgcatcttg gatccccggt gtgagaattt tacgcctggc
     2281 agaggttcgc gttcggctgg aatagtccac atggaattgt caatatcaat ttccgaccag
     2341 cgggcatatc tgagttcact ggaacgaata aaaaccagta aattcagcat aactgccagt
     2401 cgggtgacag gctggccttt ataaccgtca atacgcgaca gaagttcagg caggcgtgaa
     2461 agctccagag cagggcgatg ctggcgtttt accgtggtca atgcaccagc catatccaga
     2521 gccgggttat atctgatgat gccagactgt acggcgtaac gcattacagc agatattctt
     2581 tgctgcagac gactggcgat ttcataaatt tgttttgctt ctgcagcacg aacagagata
     2641 agcaggtccg gggtattgag tgttgtgata tcccggttgc cgataaaggg gaaaacgtgt
     2701 gtctcgaggc ttttgagtac tttatcgctg tgtgattgtg cccattgttt attactggca
     2761 tgccagcgtc gtgcaatatg ttcaaaagag agctgtaaat cagggacaac ttttttaact
     2821 cttttctgtt cgcaagggtc aatcccctct gaaatcagtt ttcgggcctc atcccgtttt
     2881 gtacgagctt cggagagaga aacttctgga tacacaccaa gtgccagagt cttctcctta
     2941 cccagaatac gataacggag acgccagtaa cgggaaccat taggatgaac cagcaggaac
     3001 atgccggccc cgtcagtgag tttataagcc ttgtcagtgg gctttgcagc ccggattttt
     3061 gcgtcagtca gtgccatagt tcagccttct gaatgcgtta aagagggtac aaattctgtc
     3121 gaactggggt atacccgcaa ttatacccac acaatgaggt tgatgtgggg agaatttggt
     3181 tgagttcggt agaattgatt tggaggaaga acacttaaat catggtgttc tgaaacgaaa
     3241 aacggacctc cgtggaggtc cgttgatatg aatttggtgc ccggactcgg aatcgaacca
     3301 aggacacggg gattttcaat cccctgctct accgactgag ctatccgggc aacggggcgc
     3361 attaaacctg attcgcctcg tctcgtcaat caaatttctt caatttactg cagactgcac
     3421 aatctatcat cactttgccg ctattgcacg cattgtcagg caaaccaggc ggtccaggcg
     3481 gcggaaagcg gcattgccag aaggagtgct ggaagcatgt tgaccaccgg gaacatttta
     3541 atgccacaga tgcgtaatcc ggttgccagc agcaataaac cgcctacggc gctgaagtct
     3601 gccatcatcg ccggtgtggt cagcggtaat atcagcgcgg cagcccacgc cagcgttaac
     3661 tggatgatca gtaatgggat actaatcacc gataccgcaa tacccagcga gcaggcgaag
     3721 atcatcgccg taaagaaatc aagaaatgac ttggcgatta aaatactcgg atcgccggtc
     3781 atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg cgcaaaacag gacaataatc
     3841 gcgacataat tctgaataaa agattcatgc gctggcttct tacgtgagtg acgaaacaga
     3901 ttttgtgctt tggtgactgc tgtattgaca cctttctcca gcaaacaaat ttcgccaatt
     3961 agagcgccaa gtagcgtagc taaaaccatc gctggaaggt tggcacattt caccaccagt
     4021 aaaataccaa tccccagcga tgccagacca aaaatagatg tcatggagac gcggatacgt
     4081 tccggtaagc gttggctgag cagtgcgccg agaacgccac ccagtaatac tgcactagcg
     4141 ttgataaaag ggccgatgac cacaagagtt cctgttaact gtattgtttg attttcttat
     4201 tatgaccgtt tggtgccgtc gatggcttgt ttgttcgcaa agtcctggct tgcacgcttt
     4261 agcgaaaggt gccatgattg cgcgaatttt ctcctctctg tacggagttt gcccgatgca
     4321 cgccacctcc ttacattctc tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat
     4381 gttactgctc atgcggtgaa gttaacgcac gctcactgca ggacaacagt aaaatcagag
     4441 cgtttctgct tttactgatg tctggcggtc ggagctggtg accagtttga cccatatctc
     4501 atggggcagg gttttccacc ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt
     4561 atgaaatcaa tgaatattgc cgccagtagt gatctggtat cccgactttc ttctcatcgt
     4621 cgcgtggtgg cgttgggaga tactgatttt acggacgtcg cggcagtcgt cattaccgct
     4681 gcggatagtc gcagtggcat tcttgcgttg cttaagcgca ccggttttca tctaccggtg
     4741 tttttgtatt ccgaacatgc tgttgaatta cctgcgggcg ttacggcggt aatcaacggc
     4801 aacgagcagc agtggctgga gctggaatcc gcagcctgtc agtatgaaga gaatttgctg
     4861 ccaccgtttt atgacacgct gacgcagtac gttgagatgg gcaacagcac ctttgcttgc
     4921 cctggacatc aacatggtgc gttttttaaa aagcatcctg ccggacgcca tttttacgat
     4981 ttctttggtg agaacgtctt tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat
     5041 ctgcttattc atgaaggatc ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat
     5101 gccgataaaa cctattttgt gctgaacggc acatcggcag cgaataaagt ggtgacgaat
     5161 gcgctgttaa cgcgtggcga tctggtgctc ttcgaccgta acaaccataa gtcgaatcat
     5221 cacggcgcgc tgattcaggc gggggcgacg ccggtctatc tggaagcttc acgtaacccg
     5281 tttggtttca ttggcggtat tgatgcgcac tgttttaatg aagagtatct gcgccagcaa
     5341 attcgcgacg ttgcgccaga aaaagccgac ctgccgcgcc cgtttcgcct ggcgattatc
     5401 cagctgggaa cctatgacgg cactgtctac aacgcccgtc aggtgatcga taccgttggg
     5461 catctgtgtg attacattct gtttgattcc gcgtgggtcg gttatgaaca atttatcccg
     5521 atgatggcgg atagctcgcc gctgctatta gaacttaacg aaaacgatcc ggggatcttt
     5581 gtgactcagt cggtgcacaa acagcaggcg ggattctcac agacgtcgca gatccataaa
     5641 aaagataacc atatccgcgg acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc
     5701 tttatgctcc atgcttctac cagccctttc tatccgctgt ttgctgcact ggatgttaac
     5761 gccaaaattc atgaagggga gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt
     5821 attgagtcgc gcaaggctat tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc
     5881 gttgttgatg gcaaattgtg gcaggattat ccgacatctg tgttagccag cgaccgccgt
     5941 tttttcagtt ttgagccggg ggcgaagtgg cacggctttg aaggatatgc cgcggatcag
     6001 tattttgttg atccgtgcaa gctgttactc actacaccag gtatcaatgc cgaaaccggc
     6061 gaatatagcg actttggcgt tccggcgacg attctggcgc actatctgcg tgagaacggc
     6121 attgtgccgg agaagtgcga tctcaactcc attctgttct tattaactcc ggcggaaagc
     6181 cacgagaagc tggcacaact ggtagagatg ttggcgcagt ttgaacagca tattgaggat
     6241 gactcgccgc tggctgaggt gttgccgagc gtttataaca aatatccggt gcgctatcgc
     6301 gactacaccc tgcgccagtt gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc
     6361 aaagacctac aaaaagcgat gttccgccag cagagtttcc cgtcagtggt gatgaacccc
     6421 caggatgcgc atagcgctta tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc
     6481 gaagggcgaa ttgcggcaga aggggcgttg ccttatccac ctggcgtgct ttgcgtggta
     6541 cccggggaag tctggggtgg ggcggttcaa cgttatttcc ttgcactgga agaaggggtg
     6601 aatttgttgc cgggattttc gccggagctg caaggtgttt atagcgaaac cgatgcggat
     6661 ggcatgaaac ggttgtacgg ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc
     6721 tggcgacccg tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac
     6781 gaacgtgttt atatttgaac atcgccatga acgcgaaggc cagaaccacg gagtaaccag
     6841 cgaaaatcaa ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa
     6901 ctttaccgct cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca
     6961 tcccttgtgc actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag
     7021 agatgttgaa gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag
     7081 taccgaacgg agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa
     7141 tactgatcat cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa
     7201 tgaacagggt ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacaataa
     7261 agctgctggc aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt
     7321 tggtaatctg cagttccgcg cccagcagca ttgagaagat aaagaagatt gccatacgct
     7381 tgtttttaaa cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt
     7441 gctgtttagc aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa
     7501 gtgctgcgcc aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc
     7561 acattgccat gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga
     7621 tatccatccc ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg
     7681 gcatataagc aaacgagtta atcaatatca caaggaacat cgcctccggt gtcgtgacct
     7741 gtgccgccat gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg
     7801 cacttaacca tttgtcggcc acaatcccca gcagcgcagg cataaatacc gcggcgatac
     7861 ccagtgagct ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat
     7921 aggagccgag ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt
     7981 tcagctgcag cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga
     8041 aaaagcacat ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt
     8101 gttacctgtg gcaaataatt gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga
     8161 taacattttt gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg
     8221 atttttgcgc ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg
     8281 tcgtcagcgt ctgataaaca tcgcccggcg tcatagtgtt aataatattg gcagcctgaa
     8341 tcttatcatt cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga
     8401 cggcataacg ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg
     8461 ccagatacgc ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg
     8521 gcgtgccgga tttcccctgc gagcggaaca catctttccc ggcagtatgt tgtaccacct
     8581 gcattaatcc cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcggtct
     8641 gcataattgc cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca
     8701 tgccgagata tttgtgcgca cgtttatcaa ggtggttcgg tagcatgtta atggtgacgc
     8761 tgtagatgat acgcagcccg ttactgcggc tttgcagacg gtttttcagc agataatccg
     8821 cgaagttgct tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac
     8881 cgtaaaggaa aggttctttc gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg
     8941 aactcggatc gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag
     9001 gttctgtccc ggcgatagtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc
     9061 gggtctgata ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt
     9121 tgaatcccca gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt
     9181 tggtatcttt gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga
     9241 tgagcaacgg cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt
     9301 gtgtttgttt ttatgcaagt tgccggaggc gtgctccggc actgttttta ttttttatct
     9361 tccggcgtat agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc
     9421 tgctccagca gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc
     9481 atggtttgtt tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt
     9541 tttcccagct cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa
     9601 gtacaaaaaa tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc
     9661 gcgccagtgc gtaactgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc
     9721 ggttgcgcta agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac
     9781 acgggaagcc acataggcac aatatctaag tggaaatggc tgaaggtatg ccgaaacgcg
     9841 gtcagctgcg tcaggttatc ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt
     9901 tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc
     9961 tgcgccagca atacttcatc ttcgtgctgt aacaggagaa agtagccggt gcgctccggc
    10021 agcgtctgtt tcggtttttt gcccggataa agcgaccagc tattgttggc ggcggcaata
    10081 catccgtttt gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca
    10141 cccaaatcca tcatcgcctg attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg
    10201 ctcaaactcc ataatttatt ctcgacctct tttttccctg gccagccgct tacagcatag
    10261 cagcgcgcca gcacccgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa
    10321 agcgagagaa tcgcacctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca
    10381 aaggtttccg ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc tttatgcagg
    10441 ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc
    10501 gcattggcga gatcggtcac cgtcgggaag cgcgccataa agcgttcaaa ataggggata
    10561 acggtcgcaa cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc
    10621 ttgtcaattt gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg
    10681 gctgaaaatt gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca
    10741 gcacagcgac ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc
    10801 atccggcaac taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc
    10861 tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt
    10921 atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa
    10981 aactactggc cggtgatggg cgttgagttc agcgaagata tgctggattt ccccgcgctt
    11041 tttggccgtg aagcgccgct gacgcttgag attggttttg gcatgggggc gtcgctggtg
    11101 gcaatggcta aagatcgccc cgagcaggac ttcctcggca ttgaagtgca ttcaccgggc
    11161 gttggtgcgt gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt
    11221 cacgatgcgg ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag
    11281 ctctttttcc ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgttcaggtg
    11341 ccgtttgccg aactggtaaa aagcaaactg cagctggggg gcgtattcca tatggcgacc
    11401 gactgggaac cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa
    11461 aacctgtcag agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt
    11521 gaacaacgtg gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa
    11581 taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg
    11641 aattaggatt ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata
    11701 aaaccgttga tgattttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca
    11761 gcggttatct ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag
    11821 aacatcaggc gattgtgcgt aagtggctgg aagagcgcaa actggatgag gtacgcacca
    11881 gcgaactttt cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac
    11941 tgcctgatgc gctacgctta tcatgcctgg aaagatgcac gatcgagtag gcgggataag
    12001 gtgtttacgc cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcaa
    12061 atgctggcct gattttttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag
    12121 cactttcagt gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg
    12181 acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga
    12241 tcacgccaga cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc
    12301 gcgagcaggc gaaggattat caggctgaac tacgcagcac gctgccgtgg attgatgaag
    12361 gcgcgaaaag ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga
    12421 tgggcgaaag cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc
    12481 agatgaaccg cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg
    12541 atcaggttcg cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg
    12601 acagcattaa tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga
    12661 atgtgctggg aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg
    12721 aaaaagattt ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata
    12781 gccgcaaagc cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt
    12841 ttttatgccg tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat
    12901 atattcacgt tgtaaattgt ttaacgtcaa atttcccata cagagctaag ggataatgcg
    12961 tagcgttcac gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca
    13021 ctggttatgg gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc
    13081 ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa
    13141 gttggcgtag aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa
    13201 ggcgagcagg tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg
    13261 gcgaaaaaaa ttaacaccga ctgcgataaa accgacggct tcgtcattac ccacggtacc
    13321 gacacgatgg aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg
    13381 gtgatggtcg gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg
    13441 tataacgcgg tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg
    13501 atgaacgaca ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg
    13561 accttcaagt ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgactac
    13621 caacgtaccc cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat
    13681 gagctgccga aagtcggcat cgtttataac tacgctaacg catccgatct tccggctaaa
    13741 gcactggtag atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg
    13801 tataaatccg tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt
    13861 tcttcccgcg taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac
    13921 ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct
    13981 ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct
    14041 cgccccggta tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat
    14101 taccctaatg ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc
    14161 aatggattgt tggcacggca ctgcttatgt tgatgactgg taatgcttgg gcagatggcg
    14221 aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca
    14281 agcttgatgc tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag
    14341 cggaaggcga tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt
    14401 acgaactgct cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa
    14461 ccagtaaagg aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag
    14521 ccagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga
    14581 atagcgaaga tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga
    14641 actcgattcc tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc
    14701 agaaagcagc ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag
    14761 aagcctttaa aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg
    14821 cgttcgcggt taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac
    14881 agagtgaaga gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac
    14941 aacggcgaca gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg
    15001 taacgctcga aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa
    15061 aagtcgcgat tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag
    15121 ataccgaaca actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcacgatttg
    15181 tagcaggatg cggcgttgac cccgcatccg gcaatcaacg tctgatgcga cgcttgcgcg
    15241 tcttatcaga cctacaaccg ttgccacatg taggctggat aaggcgttca cgccgcatcc
    15301 ggcaatacaa gtttactcag ccagaaaaag ctccagcagc gaatttaaaa acagcttccc
    15361 atgttccgtt atctgccagt aatccgcaca ttcggtgaga taaccctggg caatcgcctc
    15421 gtctaactgt gggcgaatca catcttcgca aagcccggta tacgcaataa actccacgcg
    15481 cggcgcggcc tccagcagac ggaagcgatt cataaagaac tcaaacggct tatctgcggc
    15541 ttcgacatca cgctggcttt ccagatacct tccctgcata aaaccacgcg gatgacgcgt
    15601 tttggtggta cgcagaatgc gcccatccgg gaaggtcact ttgccgtgcg cgccacagcc
    15661 aataccgatg tagtcgccaa atcgccagta gttgagattg tgctggcact gataaccggg
    15721 tttggcgtaa gcggaagttt catattgctg ataacccgct gcggttaata actgatgccc
    15781 ctgttcgaaa atatcccaca gcgcgtcatc gtccggcagc accggtggtc gcgaaccaaa
    15841 cagcgtattg ggttcgatgg tcagttgata ccaggaaaga tgcggcggat tcagttcaat
    15901 ggcctggcgt agatcgccaa gcgcctcttc cagtgattga tccggtagtc catgcatcaa
    15961 atcaaggtta aagctacgta accctaaacc gctcgccagc tttgccgctc gtttcgcttc
    16021 ttgcgggcca tgaatgcgcc cgagtcgttt cagcttttct tcgctaaaac tctgcacacc
    16081 aatagagatg cggttcacac cagcacgctg ataatcgaca aagcgatccg cttctaccgt
    16141 gcctgggttc gcttccatag taatttctgc atccgctgcc agcggcaaac gcgcacgcac
    16201 gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg ctcggcgtac caccgccaat
    16261 aaagattgtc tttacttcac ggccctgagc gtaagccaca tcgttgtcca gatcgttaag
    16321 cagatgctga acataatcgt cgtgcggcac ttctcctttc aacgcgtgag agttgaaatc
    16381 gcagtacggg catttctgca cgcaccacgg gatgtgaatg tagagactca gcggcggtaa
    16441 tttaaccatt acgtaaagcg tccagcagca gtttcaatgc ctgaccacgg tgggaaatgg
    16501 cgctcttttc ttcgcgggtc agttcggcgg cggttttccc ttcggaaggt acgaagaaga
    16561 ttggatcata accaaagcca ccagtgcccg ctggttcacg agtaatcacg cccggccagc
    16621 taccgtggca taccagcgga gtcggatctt ccgcgtgacg cagatatacc agcacgcagt
    16681 ggaaacgcgc ctgacgttgg tcatccggta cgtctttcag tgtttccagc agtttttgca
    16741 gattctttag atcggtcgcg tcttcaccgg aataacgcgc ggagtaaatg cccggcgcgc
    16801 cgccaagcac atctaccgcc agaccagagt cgtcggcaat tgccggtaaa ccggtcactt
    16861 ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa ggtcaggccg gtttcttcag
    16921 cggaatctac gccgaggtct gtttgggcca cgatatcaag accgaagtcg ctaagcagcg
    16981 acgccagctc acgcacttta ccggcattgc cggttgcgag gacaactttt tgcataggat
    17041 acctaattaa ttaacgccgc gatttctggc gggatttgtt gcggattaat gattttaatt
    17101 tgtttgtggc ggccaagttc gcctttttca atcaccacct ggcttttggc aacccggaat
    17161 tgcttaccga gaaacttcac cagatgactg ttggcctgac cgtcaaccgg cggcgcggta
    17221 atggcgactt taacttcgtc gccatgtaaa ccgacaatag aatcacggct ggctttcggc
    17281 tgaatataga gccgtaaaac cagaccgtca tcattaactg ttacggcact cataacgcca
    17341 tccacagccc cggcagcagc atgtttccgg ttgcctgtaa tacttctgcg acacccatat
    17401 tgatgacata cagcagcaga acgaggatca tcggcgagaa atcaatccca cccattgccg
    17461 gtagcaagcg gcgaatcggg cgcagcagcg gatcggccag ctgaatcagc acgtattcaa
    17521 tcgggctacg accctggctt acccagctca taatcgccat caccagcagg acccagaaaa
    17581 tcagcaggcc gatggttttc agcagaatca gtaaaccagc aatccagatg attggcagga
    17641 aggtcaccac tttaaacagc acgatggctt tgataaaact gagaatatag gcaaccagca
    17701 gcgaggcgct gtcaatgggt cccattgccg gaataacgcg gcgcagtggc ccgataattg
    17761 gctgcgttac cttcactaca aactgtgaga aggggttgta aaaatcacaa tgagcccact
    17821 gcatccagat gcgtaataac agcaccatgg tatacagctc aatgaccgtt gaaagcagga
    17881 aagtcaacgt attcatggcg ttcctttaat tccttatttt ttagagtaat cacgcgcacc
    17941 aaaaattgca gtgccgatac gaaccatcgt gctacctgcc gcaatggcgg cttccatatc
    18001 gtccgacatt cccagagaga gcgtgtcgat atgcgggtag cgcgttttca gtccggcaaa
    18061 tgctacagcc atttggcgtg caacttcaaa ctgccttaca tattctgact caggcgcagg
    18121 gattgccatc agcccgcgca gacgtaaacg cggtagttca gcgaccgccg ctgccagctc
    18181 gtccagttct gccagttgaa tcccggactt actgttttca tcactaatat taatttgaat
    18241 cagtacgtta agagggggaa gttctgccgg gcgctgatcg ttgaggcggg tagcaatgcg
    18301 caaacggtcg atggtatgac accagtcgaa atgctctgcc accaggcggc ttttattaga
    18361 ctgcaacggg ccaataaaat gccattctaa tcctgttacg cccagttcct gaaagtggcg
    18421 aattttatct accccttcct gaacgtagtt ttcaccaaat tgacgctgcc cggcatcaat
    18481 ggcttctgcg atggcgctcg caggttttgt tttactgact gcaagcagcg taatttcttc
    18541 tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg tcccggacct gtgccaggtt
    18601 atgcgcaata tcgttcattt tccgaggatc ttagtatgaa tatggaagaa attgtggccc
    18661 ttagtgtaaa gcataacgtc tcggatctac acctgtgcag cgcctggccc gcacgatggc
    18721 gcattcgcgg gctaatggaa gctgcgccgt ttgatgcgcc ggacgtcgaa gagctactgc
    18781 gggagtggct ggatgacgat cagcgggcaa tattgctgga aaatggccag ctggattttg
    18841 ccgtgtcgct ggcggaaaac cagcggttgc gtggcagtgc gttcgcgcaa cggcaaggta
    18901 tttctctggc attacggttg ttaccttcgc actgtccaca gctcgaacag cttggcgcac
    18961 caacggtatt gccggaatta ctcaagagcg agaatggcct gattctggtg acgggggcga
    19021 cggggagcgg caaatctacc acgctggcgg cgatggttgg ctatctcaat caacatgccg
    19081 atgcgcatat tctgacgctg gaagatcctg ttgaatatat ctatgccagc cagcgatgtt
    19141 tgatccagca gcgggaaatt ggtttgcact gtatgacgtt cgcatcggga ttgcgggccg
    19201 cattgcggga agatcctgat gtgattttgc tcggagagct gcgtgacagc gagacaatcc
    19261 gtctggcact gacggcggca gaaaccgggc atttggtgct ggcaacatta catacgcgtg
    19321 gtgccgcgca ggcagttgag cgactggtgg attcatttcc ggcgcaggaa aaagatcccg
    19381 tacgtaatca actggcaggt agtttacggg ccgtgttgtc acaaaagctg gaagtggata
    19441 aacaggaagg acgcgtggcg ctgtttgaat tattgattaa cactcccgcg gtggggaatt
    19501 tgattcgcga agggaaaacc caccagttac cgcatgttat tcaaaccggg cagcaggtgg
    19561 ggatgttaac gtttcagcag agttatcagc agcgggtggg ggaagggcgt ttgtgacgtt
    19621 gccatgcccg atgcgcaagt ttatagggcc tataaaggca ataaatgtct gatgcgacgc
    19681 taacgcgtct tatcagacct acagccgctg ccagatgtgt gctggataag gcgttcacgc
    19741 cgcatccggc aagatggcgg taacgactat ccgccaggct tttagatcgc cttaatatcc
    19801 ctgctcgaaa tagctttcga gaataataac cgcagaggca gagtcgactt tgcctttgtt
    19861 gagcgcccga tagccgccct gttcaaacag accggaacgg gcttccacgg tgctaagacg
    19921 ttcgtcatgg agctttactt caacaccgaa acgaccatga atacggttgg caaatttacg
    19981 cgctctggca gtcaatggtt gctcggtgcc gtccatattc agcggcaaac cgacgatgat
    20041 ttcgtccggc tgccactctt tcagtaaacg ttcgatgagg ttccagtccg gcgtaccgtc
    20101 ctgtgcttta attgcaggca aagggcgagc agtgccggta atgcgttggc cgaccgctac
    20161 gccaatgctt ttggtgccga agtcgaaggc gagtaaggtt ccactcatca ggcgtgccct
    20221 gccacaccag gcatggtgag aatatccaca ccaatcagtt ttgccgcctc gcgccagcgg
    20281 tcggcaatcg gcgttttgaa cagaatattc agatccgccg gggccgttag ccacgcgtta
    20341 tcgagaattt cttgttccag ttgacctttt tcccaggagg cataacccag agccaccaat
    20401 acgtcagacg gttgtttatc ggtgccgagc gtttccagca catcgcggga agtggtcatc
    20461 actgtgttgt ctgaaatgcg aatgctggaa gcaaaattgg agggcggagt atgcaaaata
    20521 aacccgcgat cttcagccag cggaccgccg agcataaccg gtttatccag acggattgat
    20581 tcatcacgcg gctccggcgt gatcttcagc ttttccagaa tcccttcaat tttgagattt
    20641 tccagcggct tgttgacgat gatccccatt gcaccattgg tattatgttc gcaaatgtag
    20701 accacggaac gacggaaaat cggatcctgg agagcaggca tggcaataag aaagtgatgc
    20761 tgtaaattca ttgtcagagg ttctgtttcc tggttcaaaa aagcaacagc gcccagtatg
    20821 cgtggaaaga agggcgctgt cactcagagt ctcaacgaga tccttctcgc taaggtgggt
    20881 tactgctgct gtaaacgtgc ttcgatggca tccattaaca ttccggtgat cgacaccgga
    20941 aactctgctt caatctcacg aatacaggtt gggctggtga cgttaatttc agtcagacgg
    21001 tcgccgatga tatccagacc aacaaaaatc agcccttttt ctttcagcgt cggcccgatc
    21061 tgacgggcga ttttccagtc actttccgtc agcggacgag gttcaccgcg accaccggca
    21121 gccagattgc cacgggtttc gcccccctgc ggaatacgcg ccaggcagta cggtaccggc
    21181 tcgccatcca ccaccagcac gcgtttgtcg ccatctttaa tggctggcag gtaattttgc
    21241 gccatgcagt agcgagtgcc atgctcagtc agggtttcgg caatcacgcc gaggtttgga
    21301 tcgccttctt tcacgcggaa aatcgacgcg ccgcccatac cgtccagcgg cttaagaatg
    21361 atgtcgctgt gtttctccca gaacgctttt agctgcgctt tattgcgcgt aaccagcgtt
    21421 tctggcgtta agtcagagaa ccaggcggta aacagtttct cgttacagtc gcgcaggctc
    21481 tgcggcttgt taacgatcag cgtccctttc tcttcggcac gttccagaat ataggtcgcg
    21541 tagataaact cggtatcaaa cggcgggtct ttacgcatca ggatcacatc gagatcggcc
    21601 agcggcagat cctgttcacc gacgaatgaa aaccactctt cgtagttctg cttcacgttc
    21661 agcgtgcggg tatgggcgcg ggcttcgcca ttgatcagat acagatcgcc catctccata
    21721 tagtgaagtt cgtaaccacg acgctgtgct tccagcaaca tagcgaaact ggaatctttc
    21781 ttgatgttga tgtttgcgat ggggtccatc acgatgccga gcttgatcat tattcttctc
    21841 cgttagccca aatcgccaaa tcgtacttgt agcgcggtaa tggcggtgag cgcagttgtc
    21901 tctgtacgca aaacgcgagg tcccaacagg atatcagtaa attgatagcg ggcagtcatg
    21961 gcaatttcat ctgccgataa accgccttcc gggccaatca gcaggcggac gcgttcaacc
    22021 ggtaacggca acgtattgat gctgttactg gcgcgcgggt gaagattcag tttcagtcct
    22081 tcatcctgct ctgcacacca ggcttccaga tccatcgctg ggcggatttc cggcacccgg
    22141 ttacgaccac actgctcaca ggcagcaatt gcaatcttct gccactgctg gagcttcttg
    22201 ttcagacgtt cactatccag tttaacgccg cagcgctcag aaaaaagtgg cgtaatgagg
    22261 cttacaccga gttcgatcga tttctggatc gtaaattcca ttttttcacc acgcgacatc
    22321 acctgaccga ggtgaatatg cagcggagat tcgcgatcgt cgatcttgcc ttccagcacc
    22381 ttcacttcca cgcttttttt gctggcgctg gtaatttcgg cgtcaaagac ctggttgcta
    22441 ccgtcaaaca attgcaacgc ctgccccggc cccatgcgca gtacgcgccc gatatggttg
    22501 gcggcatctt cgcaaagcgc gatgtgagaa tggctggtca gtggttcagg atgataaatg
    22561 cgggggatac gcatagttaa aaatccgcgt cgtctcccca cgcggttgta cgcgtggggt
    22621 aggggttaac aaaaagaatc ccgctagtgt aggttagctc tttcgcgcct ggcaagcgcg
    22681 ttgcacatac gggttatgat tgccctgcac cttcgcgatg cgttcatcgc gttcgcactc
    22741 ccagtcggta accggataca tcttgttcca tgcgttgaac agctgcgttt gctggcgaga
    22801 gagtgtcagg ttgtattggt cgcgcatata gaagtaggtg cgtgcaatgg caccgcgtgc
    22861 acgcgctggt ggttcggcag ctttttcttt gaaatcgacc ttcatggcgc attgaccgta
    22921 ctggccttca ccgccattcc actggctgta cataaagttg ccgcgatcgc cattcacctc
    22981 accgactgac ggttgcaggt tatgcatatc gctttccatc ttgcgataga ccggatcttt
    23041 agcgcagttt ttacgtccac cgtcctgcca gcactggcgc tggtgaccga actgccaggc
    23101 gggaacgacg tgttcccact ctacgcggct ggcgcggttt tcatttttac gcacctgata
    23161 gccgcacgat tgcagatcaa caacgccttt tttgccctgc cagttaattt tacatccgca
    23221 ataaaacgta ccgggcgcgt cagcgtggac ttttaccgcc gcggctttcg cctgagaaaa
    23281 actattgata ccttcggcca acgccgggcc ggaaaatgct gcgctcagta ccaccgcagc
    23341 aatagacaaa taacggtaca tcacttactc cgtgttaaaa cgagccagca acgtagcgaa
    23401 agttgttttg gtatgcaatc agccagatca ggaaagttcc tgatagttca gatggttatt
    23461 tcgcaaccag ctgttcaccg cagtgaacac agcgatagac ggcctcgcca cgaactacgc
    23521 gattatggcg gcgtacggta agctgatgct cctggcactt gcagcggtag gggaaggtgt
    23581 tgcgacgcac ggattgcagt tcgaactgat gcgtacgacg ggcgggaacg cccagcacgc
    23641 tttccatcat ccacttccac tctttgccat gtggtgctac gcggccgaaa tgtttccata
    23701 ccagcaaatg tgccagttcg tgcggcacca cttcttcaat aaaggcttca ctgttttcca
    23761 acagcaaaac gggattgagg cgaatttcat agctttccag ccaggccgtt ccggcggagg
    23821 ttccgcgctg ggtgtaagag agttttggct ccgggtagtt acgccctagc ttcaggttgg
    23881 cctgggcgag tttttcccgc aggcgacgca taacggcctg ttggatggcg atagggagac
    23941 gggatgtttt catagcggca gaggatagag cgaagaaaag atgactgcaa gaggtggctt
    24001 cctccgtgac aggaggaagc ctgaggagat taatcgtgag cgccgatttc gcgcagttta
    24061 cgacctttca tcagattacg ttcaatatgt tccagcgaaa cgtgtttggt ttccggtacc
    24121 agccacaatg tcagcaggat aaacagtacg ttcagagccg catacaccca gaaggtgttg
    24181 gcgttaccca gcgtgttgag catggtcagg aacgttgcgc caacgatcat gttggcaatc
    24241 cagttggtgg cggtggagca ggtgatgcca aaatcgcggc ctttcagcgg ctgaatttcg
    24301 gagcacagta cccaaatcag cggaccggca ctcatggcaa aaccgacaat aaacatcagc
    24361 agcatggcga tggcgaaata ctgcgctgac ggagagtgaa taccgatatg catcattgta
    24421 ccgagtacgc ccatgccagc agccatcacc aggaagccca gcgttagcgt tggtttacgt
    24481 ccccagcggt caacaaggcc gattgcgatg aaggtggcaa gtacgttggt caggccgaca
    24541 atcactgtcc cccacatttg ctctgtggtg ttggtataac ccgccagttc gaagattttc
    24601 ggcgcgtaat acatgatgac gttcatcccg gtgaattgct gcattacctg taacagtacg
    24661 ccaaggaaca ccgcgcggcg gaagttgctg ttctctttaa acagcgccca gccactctgt
    24721 ttaacctgca aactttcacg gatttcatcc agttcgcgtt tcgcttccgc gctggtgtca
    24781 cgcaggcgta gcagcactcg ttcggcatca acaaaacggc gtttggcggc aaaccaacgt
    24841 gggctgtctg gcaggaagaa gacaccaatc agcagcaaaa ttgccgggat gataatcaca
    24901 cccagcatcc agcgccatgc accggtgtag ctgaaggcgg tatcagaaag ataagcaccg
    24961 aggatcccga tagtgatcat caactgatac atcgagatca tactgccgcg aattttttcc
    25021 ggcgcaattt cagagaggta cagcggtgcg gtataagagg ctacacccac cgccagcccc
    25081 agcagaacgc gggaaagaat cagtacttca acgtttggcg cagccgcaga gaacagcgaa
    25141 ccggcaacaa acaaaattgc gccgatcatc aggctctttt tgcgcccgag tttaaaggag
    25201 agccagccgc tgcccaccgc accgactgcc gcaccgaaca tcatggagct tacgacccat
    25261 tcttgcgtgt gcgaagtaat ctggaattca tctgcaataa acggcagtgc gccagcaatt
    25321 acaccgatat ccaggccaaa gagtaatccc gccagagcgg caaggaagca gacgaaaaaa
    25381 gtcattgcct tgtttgaccg cccctgtttt ttagcgtcag gcatgatgcc ctccaatatg
    25441 gttatttttt attgtgaatt aagataggtg agtacgacgt aaaaagatgt gaagcaaatc
    25501 acatcagtg
//
LOCUS       DAPPNJ010000053        25252 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000053 DAPPNJ010000000
VERSION     DAPPNJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.053"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(106..858)
                     /gene="yecC"
                     /locus_tag="RPM73_001785"
     CDS             complement(106..858)
                     /gene="yecC"
                     /locus_tag="RPM73_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDX6520156.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="RPM73_001786"
     CDS             complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="RPM73_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDX6520157.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="RPM73_001787"
     CDS             complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="RPM73_001787"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDX6520158.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="RPM73_001788"
     CDS             complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="RPM73_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6520159.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="RPM73_001789"
     CDS             complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="RPM73_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDX6520160.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDHLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="RPM73_001790"
     CDS             complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="RPM73_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDX6520161.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4998..6491)
                     /locus_tag="RPM73_001791"
     CDS             complement(4998..6491)
                     /locus_tag="RPM73_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="structural flagella protein; individual Salmonella
                     serotypes usually alternate between the production of 2
                     antigenic forms of flagella, termed phase 1 and phase 2,
                     each specified by separate structural genes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDX6520162.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6746..8158
                     /gene="fliD"
                     /locus_tag="RPM73_001792"
     CDS             6746..8158
                     /gene="fliD"
                     /locus_tag="RPM73_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDX6520163.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8173..8583
                     /gene="fliS"
                     /locus_tag="RPM73_001793"
     CDS             8173..8583
                     /gene="fliS"
                     /locus_tag="RPM73_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDX6520164.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8583..8948
                     /gene="fliT"
                     /locus_tag="RPM73_001794"
     CDS             8583..8948
                     /gene="fliT"
                     /locus_tag="RPM73_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDX6520165.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9026..10513
                     /gene="amyA"
                     /locus_tag="RPM73_001795"
     CDS             9026..10513
                     /gene="amyA"
                     /locus_tag="RPM73_001795"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDX6520166.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10547..10960)
                     /locus_tag="RPM73_001796"
     CDS             complement(10547..10960)
                     /locus_tag="RPM73_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6520167.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11147..12352
                     /gene="yedE"
                     /locus_tag="RPM73_001797"
     CDS             11147..12352
                     /gene="yedE"
                     /locus_tag="RPM73_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDX6520168.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12349..12582
                     /gene="yedF"
                     /locus_tag="RPM73_001798"
     CDS             12349..12582
                     /gene="yedF"
                     /locus_tag="RPM73_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDX6520169.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12689..13357
                     /locus_tag="RPM73_001799"
     CDS             12689..13357
                     /locus_tag="RPM73_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDX6520170.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(13750..14064)
                     /gene="fliE"
                     /locus_tag="RPM73_001800"
     CDS             complement(13750..14064)
                     /gene="fliE"
                     /locus_tag="RPM73_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDX6520171.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14279..15937
                     /gene="fliF"
                     /locus_tag="RPM73_001801"
     CDS             14279..15937
                     /gene="fliF"
                     /locus_tag="RPM73_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDX6520172.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15930..16925
                     /gene="fliG"
                     /locus_tag="RPM73_001802"
     CDS             15930..16925
                     /gene="fliG"
                     /locus_tag="RPM73_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDX6520173.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16918..17604
                     /gene="fliH"
                     /locus_tag="RPM73_001803"
     CDS             16918..17604
                     /gene="fliH"
                     /locus_tag="RPM73_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDX6520174.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17604..18977
                     /gene="fliI"
                     /locus_tag="RPM73_001804"
     CDS             17604..18977
                     /gene="fliI"
                     /locus_tag="RPM73_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDX6520175.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            18996..19439
                     /gene="fliJ"
                     /locus_tag="RPM73_001805"
     CDS             18996..19439
                     /gene="fliJ"
                     /locus_tag="RPM73_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDX6520176.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19436..20563
                     /gene="fliK"
                     /locus_tag="RPM73_001806"
     CDS             19436..20563
                     /gene="fliK"
                     /locus_tag="RPM73_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDX6520177.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHKPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            20668..21132
                     /gene="fliL"
                     /locus_tag="RPM73_001807"
     CDS             20668..21132
                     /gene="fliL"
                     /locus_tag="RPM73_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDX6520178.1"
                     /translation="MTDYAISKKSKRSLWIQILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21137..22141
                     /gene="fliM"
                     /locus_tag="RPM73_001808"
     CDS             21137..22141
                     /gene="fliM"
                     /locus_tag="RPM73_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDX6520179.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22138..22551
                     /gene="fliN"
                     /locus_tag="RPM73_001809"
     CDS             22138..22551
                     /gene="fliN"
                     /locus_tag="RPM73_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDX6520180.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22554..22919
                     /gene="fliO"
                     /locus_tag="RPM73_001810"
     CDS             22554..22919
                     /gene="fliO"
                     /locus_tag="RPM73_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDX6520181.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            22919..23656
                     /gene="fliP"
                     /locus_tag="RPM73_001811"
     CDS             22919..23656
                     /gene="fliP"
                     /locus_tag="RPM73_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDX6520182.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23666..23935
                     /gene="fliQ"
                     /locus_tag="RPM73_001812"
     CDS             23666..23935
                     /gene="fliQ"
                     /locus_tag="RPM73_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDX6520183.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23944..24729
                     /gene="fliR"
                     /locus_tag="RPM73_001813"
     CDS             23944..24729
                     /gene="fliR"
                     /locus_tag="RPM73_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDX6520184.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25019..>25252
                     /gene="rcsA"
                     /locus_tag="RPM73_001814"
     CDS             25019..>25252
                     /gene="rcsA"
                     /locus_tag="RPM73_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDX6520185.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNT"
BASE COUNT         6205 a         6484 c         6485 g         6078 t
ORIGIN      
        1 tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac gcttgaaatg
       61 gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt gcagcagaaa
      121 cttctcgagg aactggcggg tgcgaggctg ctggggatca gcaaataacg cttttgctgc
      181 cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa catcccgggc
      241 aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca gctggcggat
      301 tgtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct cgtcaaacaa
      361 aatcacctca ggacgcattg ccagcgcacg cgcaatcgca acacgctgct gttgaccgcc
      421 agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg ccagcagctc
      481 gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat
      541 aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac
      601 gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat
      661 agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat ttatgctgcg
      721 tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt cgccaggctt
      781 tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca ccaggttctt
      841 aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt ctcaaaatgg
      901 ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc cgccaggtac
      961 atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa cagctccggc
     1021 acctggattg tcgcggccag cgacgtatcc tttaccaggc tgataaagct gttcgacagc
     1081 ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt ctgccatggc
     1141 gtcataccaa tactggcagc tgcttcccac tgacctttat caatagaaga aatggcggcg
     1201 cgcagtgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga
     1261 atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa cagttgtgcg
     1321 atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag
     1381 attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa aaacatgccg
     1441 ccgatactga gttgcagcgt atacccagcc cctttcaaca ggaacggcaa ggaatcaata
     1501 accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga tgataggcga
     1561 acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt
     1621 tctgactgat accgccaatc agccccgcca tcgcttttcc ggtatacaca ggatcaagca
     1681 gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc
     1741 cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca
     1801 gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg gcaacggaac
     1861 gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca
     1921 gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata ttaaccgccc
     1981 cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg ccaagagcat
     2041 tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca
     2101 gctcttccag ttgggcattg ggatcggtca gtgcatcgca catttcaatc tgggtattga
     2161 acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag
     2221 gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcgactgcg gcagtctggc
     2281 gcacatggtt agactggatc gcgccggcag taatcagcgt atcggcacct tcgcgcaggg
     2341 catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc atgggtgtga
     2401 cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt
     2461 cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa ttatgcagtg
     2521 gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt
     2581 aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac
     2641 atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt gcatttccgc
     2701 aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga
     2761 ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt tcttcaccag
     2821 atccagcgcc gccagacgat caacgaggat cgcatcgata cggcctacgc gcagatcctg
     2881 atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat tctgccgcag
     2941 ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca gatcagcagc
     3001 cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg aaatggtgta
     3061 aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct gattaatcac
     3121 cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag
     3181 tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt
     3241 taatttgccg tcatccccct gaaaactgaa cggcggataa gttccttcca gccctaccag
     3301 caacgtgccg cgctctttga ctttattaag cagaccttca tctgcaaaac ttttaacgct
     3361 catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc ccagatgtgc
     3421 taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt
     3481 aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag
     3541 gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt gatacttccg
     3601 taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc
     3661 aaggaaaccg tcctgcaggt gatcgaggga aatattttgc tcgtgtagat gattccccaa
     3721 acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa
     3781 aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc agtgtaaatc
     3841 accgcaaaat cggcacaatg ccgcccatga tttttgttcc gtttgccagc cattttcatc
     3901 aagcagcgtg tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa
     3961 ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc
     4021 tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg
     4081 gtagtcaaag ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa
     4141 ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc
     4201 gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg
     4261 ctccggcagc gtttcgatgg cttccatcac ccgctggcgc agattactgt ccagcagttg
     4321 ttgtagcggg ttttctcgct gatgatcatc agtaaccaat tcgatgctat cgccgtgctc
     4381 ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg
     4441 ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc
     4501 aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct
     4561 gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac
     4621 tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa
     4681 cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg
     4741 caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc
     4801 cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt
     4861 atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag
     4921 gggttatttg ggggttaccg gtaaattgcg gacagaaaaa accccgccgg tggcggggtt
     4981 tgagcgataa gtgtaaatta acgcagcaga gacagtacgt tctgcgtggt ctggttagcc
     5041 tgcgccagaa cagaggtacc cgcttgttgc aggatctgcg cacgagacat gttagacact
     5101 tcggtcgcgt agtcagcatc ttcgatacgg ctacgggcag aagacaggtt gtttacggtg
     5161 ttgccaaggt tggtgatggc agagtcgaaa cgattttgaa ttgcacctag gttagaacgc
     5221 aacgagtcaa cctgagacag tgctttatcg atagcagcca aaggatcttc tgttttctgc
     5281 gtagaaattg ctgcaataga atcagttttc aggctcttac cttgtaaatc actaacactg
     5341 taagttttgc catcggttgc agttacaact tcggttttgc catcgcttcc acccagttca
     5401 actttaacat tgtcgtgttt gacaccgtca gcatcagtgt aagacattgt tttaacggaa
     5461 actgctcctg ttttctgatc aacattcgct gcatactgtt taccgtcatc accaatcaac
     5521 gcaaatgaac cagtatcttt gccattggcg tcttctaact taaagagttt tgcacctgtg
     5581 atagaagcgg ttgcggtatc agtacccaac tgaacatcaa tagccccctt ccctttcacc
     5641 gttgtgactt cctttaaatt accagcttga ggctgggtac ccgctagagc ccccagcttc
     5701 agcttacctg catcaggaga tgtcggatca tcctccacag taacttcata ataaccattt
     5761 tcagcgggtt tatctgaacc ggcaacttta acgtacaact taccgttagc atcagacata
     5821 atattaccgt cacgttggcc cggagtcgta gtgcctttaa ctgtagctgt aatatctgca
     5881 tcaagtgtat tagcgtcagc aagcttaact gtcgatccaa aataggttgt ttcagccgtt
     5941 gcggctttta atttagtagg atctttaata ccaaaaccag ataatcccag tgtgtcggaa
     6001 ttaatttcct gcagtttaat agtaattgtt tcgccgtcgt ttgcaccaac ctgaatagtc
     6061 atatcctgat cagaagccag cactttaacg ccgttaaact gagtctgacc agaaacacgg
     6121 tcaatttcac tcagacgctg ctggatttcg gactggatgg aggtcaggtc actgtcagag
     6181 ttagtaccgt tagttgcctg aacagaaagt tcacgaatac gctgcaggtt gttgttaatt
     6241 tcattcagcg caccttcagt ggtctgcgca acagaaatac catcatttgc gttacgggaa
     6301 gcctgggtca gacctttaat atttgccgta aaacggttag caatcgcctg acctgctgca
     6361 tcgtcttttg cgctgttaat acgcagacca gaagacagac gctcaatagc agagctaaga
     6421 gaagactgag atttgttcag gttattctgg gtcaacagcg acaggctgtt tgtattaatg
     6481 acttgtgcca tgatcttttc cttatcaatt acaacttgat gttattgggc tgttgcccac
     6541 ggtttctcac cgtacccctt gtatcggcac ctgaatttcg aactttagaa aattttagtt
     6601 tctctcctgt tttttcttaa cggctgaaat atccgtaata tacaactcta ctctgcgcat
     6661 catcttcgtg aatcaactat taaactttgc gcacaaattg ccgataaccc gcttatctac
     6721 tgtttgcaat caaaaggaat taggtatggc ttcatttaca tctcttggcg ttggttcgaa
     6781 tctgccgctg gatacattgc taaacaacct gaccatcgct gaaaaaaaac gcttaaaccc
     6841 aattactcag caacagagtg acaataccgc acgtctgaca gcctatggca cattaaaaag
     6901 tgcattagaa aaattccaga ctgcaaatac tgcgttaaat aaagccgatc tgttcaaaag
     6961 cactaccgtt accagtagca atgaagacct taaagtttcc accgaagcag gtgctgcccc
     7021 cggtatctat accatcagcg taacccaact ggcgcaggcg caatctttac gaactgattc
     7081 cccgacaatc attgcatcaa caaaagatgc actgggtgat gagtcatcag atacacgcac
     7141 aataaaaatt acccaagacg gccgtaaaga gccgctggaa atcaagctca ataaagatca
     7201 gacgtcattg gatgagatca gcaaagccat taacgatgcc gatagtggaa tttccgctag
     7261 tatcgttaaa gtcaaagatg gtgactatca attggtattg accgccagtg aaggtctggc
     7321 caataaaatg accatatctg ttgagggtga cagtaaatta aatgatctgc tggcttatga
     7381 cagcaagacc aacaccggga atatgaagga actggttaac gcgcaaaatg cacagctaaa
     7441 cgttaacggt atcgatattg aacgttcaag taacaaaatt accgatgctc ctcagggagt
     7501 gactctcgat ttgaccaaaa aagtcactga cgtccgggtc acggtgacaa aaagcaacga
     7561 taaagcaacc gaagcaatta aaggctgggt ggattcatat aactccctca ttgacacctt
     7621 taacaccctg acaaaatata aagaagtaga cccaggtgct gaagcacaag ataaaaataa
     7681 tggtgcactt ctcggagaca gcgttgtacg tacaattcaa agcggcatcc gcgcacaatt
     7741 tgcaaacggt gccagcgacg gtgccttcaa aacattaaac gagatcggga ttaagcagga
     7801 cggcacgacg ggtaagctga aaattgatga tgataaactc aaaaaagtcc tgaatgaaaa
     7861 tacagcatcc gtacgtgaat tgctggtagg cgatggtaaa gaaacaggaa ttaccaccaa
     7921 aattgccacc gaggtaaaag gttaccttgc cgatgatggc attattgata gcgcacagga
     7981 cagtattaac gccacactga aaaaactgac caaacagtat ctatccgtca gtgccagcat
     8041 tgatgacacc gtggcgcgtt acacggcaca gtttacgcaa ttagatacca tgatgagcaa
     8101 actgaataac accagtacct acctcagcca acaatttact gcaatgagta attcctgatc
     8161 acggaggtca gcatgtatac cgcaagcggc tcaaaagcct atgcacaaat tggcgtcgaa
     8221 agcgccgtaa tgagcgccag tcaacagcag ctggtcacca tgctatttga tggagtgctg
     8281 agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcaacaagg caaaggcgtc
     8341 tcattgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaagag
     8401 agcaaagacg aattaaccca aaacttgatt gctctttata gctatatggt caggcgtttg
     8461 ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaaac attaatgcgc
     8521 aatattgccg atgcctggaa agagtcgtta ctctcccctt ctttgattca ggacccagtc
     8581 tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
     8641 tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc gaaatggcgt
     8701 atgttaatgc ggtgcaggag attgcacatt tgactgaaga gattgacccg tccaccacga
     8761 tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc aaggtaaagc
     8821 agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
     8881 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc
     8941 tcttttgaat ctgaatgagt cgatggctcg cgaataatcc gattacggct acgcttctaa
     9001 tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
     9061 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg acggttttaa
     9121 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcgggta
     9181 ttcggtcggc tacgactcct atgatttatt tgatttaggc gagtttgatc agaaaggcag
     9241 catccccact aaatatggcg ataaagcaca actgctggcc gccattgatg ctctgaaacg
     9301 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
     9361 agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat
     9421 cattgagtgt gaaggctgga cgcgttacac cttccccgct cgtgccgggc aatactcgca
     9481 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga
     9541 tggcattttt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
     9601 tgaattaggt aatttcgatt atctgatggg cgagaatatt gattttcgca atcatgccgt
     9661 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt
     9721 tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga tcgaacacgt
     9781 acaggaagtt gcgccaaagc cgctgtttat tgtggcggag tactggtcgc atgaagtcga
     9841 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg atgcgccgct
     9901 gcagatgaaa ttccatgaag catcgcgcat ggggcgtgac tacgacatga cgcagatttt
     9961 cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg ccaatcacga
    10021 cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcata
    10081 tgccttaatt ttgttgcggg aaaatggcgt tccttcggta ttctatccgg acctctacgg
    10141 tgcgcattac gaagatgtcg gcggtgacgg gcaaacctat ccgatagata tgccaataat
    10201 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacatt
    10261 atttttcgac catccgaact gcattgcctt tagccgcagt ggcactgacg aatatcccgg
    10321 ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattaatc tgggagagaa
    10381 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg tagtaaccga
    10441 cgaaaacggt gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    10501 agaggtgatt taaattcatc cccggcggca agccggggag atttcattac ggcagtggcg
    10561 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    10621 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    10681 gttttacggt aacgttagtg agatcatcag acatcaatgt caacttgccc ggcacggcaa
    10741 tcacccgctg ccactgacgg caatccaggg tatcgccctc tttagtgaca atcaggctgc
    10801 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    10861 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    10921 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    10981 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt cagagagaaa
    11041 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atgatgtatg
    11101 attcgcgatt cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    11161 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    11221 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    11281 tacccgttgg ggcggtcagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    11341 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga tgatgatcct
    11401 cggcatgttt ggcggctgct ttgccgcagc actgtgggcc aacaatgtca aactacgcat
    11461 gccgcgcagc cgtatccgca ttatgcaggc cattattggc ggcattatcg ccggttttgg
    11521 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggcattc ctcagttctc
    11581 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt
    11641 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    11701 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    11761 cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa cccaaactgg ggctggcaat
    11821 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcagc
    11881 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttattggtat
    11941 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    12001 gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    12061 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    12121 ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg atgatttcgc
    12181 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg
    12241 tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta tcggctggga
    12301 aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc
    12361 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaact
    12421 cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca
    12481 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gctggatatt
    12541 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    12601 gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc ctctcctatt
    12661 cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat cccaaacgcg
    12721 tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    12781 acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    12841 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    12901 gccgctgatt aacgcccgcg tagaaactgc ggccaccagt cgtatgttta aaccgctctg
    12961 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    13021 caaaaaacag ccttatttta tctatcgcgc tgatggacaa cctgttttta tggccgcgat
    13081 aggtagcaca ccgtttgagc gcggtgacga agccgaagga tttttaattg tcactgcagc
    13141 agcagaccag ggtctggtag atattcatga ccgccgcccg ctggttctgt cgccagaagc
    13201 tgcacaggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa ttgctaccaa
    13261 cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg taggtaatgt
    13321 caaaaaccag ggagcggagt taattcaacc ggtttgataa gccaggagac tattgatcaa
    13381 taatactgcg tcataagaaa tctctattag acaaagtaga cgctccttcc acatgcgcca
    13441 caagaaacct ctattccagt gacacaatta cgcctgatta tttccctagc ccggaatatg
    13501 aataaaaaca tcaacaaaga ttaaattatt caaacgttac tgttgcgtct gcggtcgtaa
    13561 caacatccga cgtggtgatg tgtatcaaat gaggcctctg atgatcagag ggtttatcgc
    13621 gtatacggat cgtggatggc agaaaacttt caggatcagg tatccatttt aaaccagaaa
    13681 ttgagtgagt ttgccccatc catgccccaa gcggtgagat tggaagtaat taacaacagg
    13741 ttacaaaacc tacacctgca tggacatcac ttcctgatac gccgccacca gcttattacg
    13801 cacctgaatc cccatttgca tagaaactga ggctttttgc atatcggtca tcacatcgtt
    13861 taacgccacg ccgggttcac cgagggtgaa tttttctgcc tgcgtgcggg cagctgtttg
    13921 tgtatcgcta atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg
    13981 cggcagtgaa tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc tgataacccc
    14041 ttcaattccc tgtatcgctg acattttcgt ctcctggata atttctggta gcaaagccta
    14101 ccagtaagtc aataagacaa aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga
    14161 tagaaaaaaa cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg
    14221 agtgagtctt gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat
    14281 gaatgcgact gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc
    14341 gaaaattcca ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct
    14401 gtgggcgaaa gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg
    14461 cgcaattgtc agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg
    14521 cgctattgaa gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg
    14581 tttgcccaaa ggcggcgcgg tcggtttcga actactcgat caggaaaagt ttggtatcag
    14641 ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat
    14701 cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt
    14761 attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg
    14821 cgcactcgat gaagggcaaa ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg
    14881 tctgccgccg ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa
    14941 taccagcggg cgcgatctta atgatgctca gttgaaatat gccagcgatg tcgaaggccg
    15001 tattcagcgg cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc
    15061 ccaggtcacg gcgcagctgg acttcgccag taaagaacaa acggaagaac agtatcgccc
    15121 taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag
    15181 cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa
    15241 taacgcgcca atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac
    15301 caccagcaat agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga
    15361 tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt
    15421 agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat
    15481 gaagcaaatt gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc
    15541 gctcaatgtc gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt
    15601 ctggcaacag caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact
    15661 gctggtggcg tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga
    15721 ggcgatgaaa gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga
    15781 agtccgcctg agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc
    15841 agaagtcatg agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct
    15901 ggtcattcgc cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg
    15961 tcatcctgct gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc
    16021 agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc
    16081 agctaaccga tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata
    16141 tcaacgccaa cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg
    16201 ccagcctgct ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca
    16261 actttatgga gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg
    16321 ccaccattct ggtgcatctg aagcgcgccc aggccgccga tattctggcg ttgttcgatg
    16381 aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg
    16441 cgctggcgga actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca
    16501 gcaaaatggg cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg
    16561 aagaagccgt tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg
    16621 acgagatgtt cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt
    16681 tgcaggaagt ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc
    16741 gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg
    16801 ccaaccgtgg tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga
    16861 ttgtgcgccg ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg
    16921 tctgataatc tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag
    16981 tttgtgccca tggtcgagcc ggaagaaacc atcattgaag aggccgaacc cagccttgag
    17041 cagcaactgg cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc
    17101 gaaggtcgcc agcaaggtca tgagcagggc tatcaggaag gactggcccg ggggctggag
    17161 caaggtctgg cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg
    17221 gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg
    17281 cagatggcgc tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg
    17341 gcactgatca aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca
    17401 cagctgcgcg tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta
    17461 agtttgcatg gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc
    17521 tccgccgatg aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt
    17581 ctggcagcac caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg
    17641 ataactttga agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaactc
    17701 gcgctaccgg gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg
    17761 tcattgagcg ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta
    17821 acggtcaacg gctgttttta atgccgctgg aggaagtcga aggtgtcctg cccggcgcgc
    17881 gtgtttatgc caaaaacatt tcggcagaag ggctgcaaag cggcaagcag ttaccgctcg
    17941 gtccggcgtt attaggtcgt gttctggacg gcagcggtaa accgctcgat ggcctcccct
    18001 cccccgatac gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta
    18061 caccgattga acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg
    18121 ggcgcgggca gcgtatgggg ctgtttgccg gatctggcgt tggtaaaagt gtgctgctgg
    18181 ggatgatggc ccgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg
    18241 ggcgtgaagt aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctcag
    18301 tagtgattgc cgctccggcg gatgtttctc ctctcctgcg aatgcagggt gccgcctatg
    18361 ccacgcgcat tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact
    18421 ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg
    18481 ccactaaagg ttatcccccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg
    18541 gaaatggcat tagcggcggc ggctcgatta ccgcgtttta taccgtgctc accgagggcg
    18601 atgaccagca ggaccccatt gccgactctg cgcgggcaat cctcgacggt cacattgtgc
    18661 tgtctcgccg actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca
    18721 gccgcgcaat gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac
    18781 agctgttgtc gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag
    18841 gcagcgatcc gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttgc
    18901 aacaaggcat ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt
    18961 tcccgacagt gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga
    19021 ccctgaaaga tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc
    19081 gtcgcggatg tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat
    19141 atcgcaataa cctcaatagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact
    19201 atcagcagtt tatccagacg ttggaaaaag ccattactca gcatcgccag caacttaatc
    19261 agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc
    19321 aggcctggca gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc
    19381 tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat
    19441 tcgcttagcg cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc
    19501 cagcgatgct gcacaagatt ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac
    19561 taccgacaaa gcggcccccc agttgctggt ggcaacagat aagcccacga caaaaggcga
    19621 gccgctggtc agcgagattg tttccgacgc gcaacaagcg gatttactga tccctgtgga
    19681 tgaaacaccg cctgtcatca acgacgaaca atccacatca acaccattaa ccaccgctca
    19741 aacgatgacg ttggctgcgg tggctggcaa caatacggca aaagacgaaa aagcggatga
    19801 tctgaatgaa gacgtcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga
    19861 caatacgccc aaagtgactg atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct
    19921 cttcacaaaa ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac
    19981 gccagctcag ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag
    20041 cacaccttca ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc
    20101 actgcccacc gtcgccgcgc ctgtgttgag tgcaccgctg ggttctcacg aatggcaaca
    20161 atcattaagc cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg
    20221 tctgcacccg caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc
    20281 acaaatccag atggtttcac cgcatcagca tgtacgcgcc gccctggaag cagcgctgcc
    20341 ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acatcagtgg
    20401 cgaaagcttt agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt
    20461 aaaccataaa cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca
    20521 agggcgcgta acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg
    20581 taatccgcgt cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc
    20641 agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag
    20701 cgatcgcttt ggatccagat tctggtattc attaccctcg cggcctgtgc cagcgcaggt
    20761 tacagctact ggcattcgca tcaggttgcc gctgaagaca aagcgcagca acgcgtcgtg
    20821 ccctccccgg tcttctatgc gctggatacc tttacggtca atctgggcga tgcagatcgc
    20881 gtactttata tcggcataac cctgcgcctg aaagatgaag ctacccgctc gcggctgagt
    20941 gagtatttgc cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc
    21001 gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc
    21061 accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt
    21121 attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt
    21181 gaatggtgac agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat
    21241 tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga
    21301 aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg
    21361 tagcccggat ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa
    21421 cctgccggtg ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct
    21481 ggtggtgttt tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg
    21541 acgcttcccg accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa
    21601 ccgcatgtta aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct
    21661 ggaagttgag tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc
    21721 gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt
    21781 taatatctgt ctgccattca gcatgattga gccgctacgg gaattgttgg ttaacccgcc
    21841 gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca
    21901 gcattcacag ctggagttgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat
    21961 tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa cccgatcgca tcatcgctca
    22021 tgttgacggc gttccggtac tgaccagtca gtacggcact ctcaacggtc agtatgcgtt
    22081 acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg
    22141 agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa
    22201 gcgttgagcg aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa
    22261 tttggcggtg gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg
    22321 gtcaagctga ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg
    22381 acgcaagggt ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc
    22441 aacggttatt taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg
    22501 atcaccgata tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata
    22561 accacgctac tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg
    22621 gcgcactcat cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg
    22681 gatttgcccc gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg
    22741 gcgcgcgtga acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta
    22801 ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac
    22861 cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa gcgtagcggg agatcctgat
    22921 gcgtcgtttg ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc
    22981 gcaactgccg ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc
    23041 ggtgcagacg ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat
    23101 gaccagtttc acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc
    23161 ctccgcgcca cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat
    23221 gtcaccggtg atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat
    23281 atcaatgcag gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca
    23341 gacccgtgag gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg
    23401 acctgaagcc gtgccgatgc gcattttgct cccggcctac gtgaccagcg agttgaaaac
    23461 cgcatttcag ataggcttca cgattttcat cccttttttg attatcgacc tggtgattgc
    23521 cagcgtgttg atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt
    23581 taaactgatg ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca
    23641 gagcttttac agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga
    23701 agcgatgaaa gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg
    23761 ccttatcatc agtattttgc aggccgccac ccagatcaac gaaatgacgc tgtcgtttat
    23821 tccaaaaatc atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct
    23881 gttgctggat tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtagcccgt
    23941 acaatgatgc aggtgacaag cgatcaatgg ctttcctggc taagtctgta cttctggccg
    24001 ttactgcgcg tgctggcgct catttctacc gcgccaattc tgagcgaacg cagcgtgccg
    24061 aaacgggtaa aactgggtct ggcaatgatg atcacgttcg ctattgcccc atcattacct
    24121 gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg
    24181 atcggcattg cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc
    24241 gaaattatcg gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat
    24301 ctcaatatgc ctgttttagc gcgtatcatg gatatgctgg cgttactact gttcctgaca
    24361 tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt
    24421 ggtggcgaac cgttgaacag caatgcgttt ctggcactca ccaaagcagg gagtttgatt
    24481 ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgctgctgac actgaatctg
    24541 gcattaggtt tacttaatcg tatggccccg caattatcca tttttgttat tggatttcca
    24601 ttaactctga ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt
    24661 tgcgaacatt tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca
    24721 ttaatataat tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct
    24781 gcgaacatct tccaggatac tcctgtggcg aaatatttgt tttaagctca ctcacatatc
    24841 gcaacattta ctttacttta agacaattcc aggcaaatta tacaacactt tacgggatag
    24901 taagtccgcc tgaaaaatcg cgagagtggc gcattaggtg acccatgttg ttccgtttag
    24961 tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag ggtatgccat
    25021 gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg ggtatctgtt
    25081 gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg accttgccat
    25141 agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt tcatccatga
    25201 tgcttctaac agtcagcaca tcaagcacat cattaatcaa catcccaata cg
//
LOCUS       DAPPNJ010000054        25224 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000054 DAPPNJ010000000
VERSION     DAPPNJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.054"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(58..2928)
                     /locus_tag="RPM73_001815"
     CDS             complement(58..2928)
                     /locus_tag="RPM73_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDX6520186.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2925..3704)
                     /locus_tag="RPM73_001816"
     CDS             complement(2925..3704)
                     /locus_tag="RPM73_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDX6520187.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3754..5082)
                     /gene="ssnA"
                     /locus_tag="RPM73_001817"
     CDS             complement(3754..5082)
                     /gene="ssnA"
                     /locus_tag="RPM73_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDX6520188.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNVDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5085..8183)
                     /gene="ygfK"
                     /locus_tag="RPM73_001818"
     CDS             complement(5085..8183)
                     /gene="ygfK"
                     /locus_tag="RPM73_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDX6520189.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8505..9083)
                     /gene="mocA"
                     /locus_tag="RPM73_001819"
     CDS             complement(8505..9083)
                     /gene="mocA"
                     /locus_tag="RPM73_001819"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDX6520190.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            9186..9956
                     /gene="yqeC"
                     /locus_tag="RPM73_001820"
     CDS             9186..9956
                     /gene="yqeC"
                     /locus_tag="RPM73_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDX6520191.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            10004..11629
                     /locus_tag="RPM73_001821"
     CDS             10004..11629
                     /locus_tag="RPM73_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDX6520192.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11670..12602)
                     /gene="arcC"
                     /locus_tag="RPM73_001822"
     CDS             complement(11670..12602)
                     /gene="arcC"
                     /locus_tag="RPM73_001822"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDX6520193.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12650..14035)
                     /gene="hyuA"
                     /locus_tag="RPM73_001823"
     CDS             complement(12650..14035)
                     /gene="hyuA"
                     /locus_tag="RPM73_001823"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDX6520194.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14088..15299)
                     /locus_tag="RPM73_001824"
     CDS             complement(14088..15299)
                     /locus_tag="RPM73_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDX6520195.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15357..16553)
                     /gene="dpaL"
                     /locus_tag="RPM73_001825"
     CDS             complement(15357..16553)
                     /gene="dpaL"
                     /locus_tag="RPM73_001825"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDX6520196.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16611..17798)
                     /gene="ygeW"
                     /locus_tag="RPM73_001826"
     CDS             complement(16611..17798)
                     /gene="ygeW"
                     /locus_tag="RPM73_001826"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDX6520197.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18277..20055
                     /locus_tag="RPM73_001827"
     CDS             18277..20055
                     /locus_tag="RPM73_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDX6520198.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(20095..20574)
                     /gene="xdhC"
                     /locus_tag="RPM73_001828"
     CDS             complement(20095..20574)
                     /gene="xdhC"
                     /locus_tag="RPM73_001828"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDX6520199.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVQDCEKTK"
     gene            complement(20571..21449)
                     /gene="xdhB"
                     /locus_tag="RPM73_001829"
     CDS             complement(20571..21449)
                     /gene="xdhB"
                     /locus_tag="RPM73_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDX6520200.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(21460..23757)
                     /gene="xdhA"
                     /locus_tag="RPM73_001830"
     CDS             complement(21460..23757)
                     /gene="xdhA"
                     /locus_tag="RPM73_001830"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDX6520201.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            24172..24927
                     /locus_tag="RPM73_001831"
     CDS             24172..24927
                     /locus_tag="RPM73_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDX6520202.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25006..25079
                     /locus_tag="RPM73_001832"
     tRNA            25006..25079
                     /locus_tag="RPM73_001832"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25038..25040,aa:Gly,seq:ccc)
BASE COUNT         5982 a         6263 c         6552 g         6427 t
ORIGIN      
        1 cactatttag tcatcgtcca aacagagaaa caggccgccg aagcggccat ttattattta
       61 tatcttttcc agcgcggtga gtattttctc cggtgtgaaa tgccattcgc gtaaccagat
      121 gccgcaggca tcgtgaattg cggtagcaat cgccggagct gcgccattta caccgatttc
      181 cgagatcgat ttcgccccga acgggccgac tttatcgtcg ctcggcacca gcactgcgcg
      241 gaaatcacgc ggaatgtcac caattttcgg tgcgccgtag ctgcgtaaat cacgcgttaa
      301 cgggtgacct tcggcgtcat aaatgatctc ttcgctcata ctgtggccga tagctcgcag
      361 ggtagcaccg tagatttgtc ccaacgctaa ctctggattg accggtgtac cgcagtccag
      421 caaggcgtag aatttatcca ggcggatttc acccgtacgc gtgttgacgg caacttcagc
      481 gaagtttgcg ccatacggga aggcgaaatc aggcgtgata taactgccag ttcccaccag
      541 tgaaccaaag ccggtgccgg tttcgccttt atgggcaata tccccgaaac taacttcgcc
      601 tttcttgccg cgcacgacgc ccggcgttgc tagttgaaca tctgccactg gctcacccaa
      661 catttgcgcg ccgtggaaca gaattttctc ccgcagattt tccgctgcca aacgcgccgc
      721 gttacccgag aagcaagtac cggacgaggc atatgcgcct ttatcaaaca acgcatgatc
      781 ggtatcaccg gagataacat gcacgtcctg cggtgggcag tgcagcactt ctgctgccag
      841 tttcgtcacc acggtatcca gaccagtacc aatatccgca ccgccagaat gaacgataaa
      901 ggtaccgtct gattccagtt tgatcatgca gttagcctga tcgatatccg ggatacccga
      961 tttctgcatg ataatggcga cgccgcgacc gatgtgccag tcaccatttt gcggtttcgg
     1021 tgacgaccat tggatcatct cgcgaccctg acgcaggatc tcttccagtg cacagctggc
     1081 ggcggaagga acggaggtcg gcgctttacc ttcaccgatt gcaccgagaa ttttcagctc
     1141 ttgcccttcg tgtacccggt tacgttcgat aatttccagt tggtcgatct gtaactgttc
     1201 agccagttcc gctaatgcca tggtgatagc gaagttacct ttcggtgcgc cataaccctg
     1261 ataagcacca tttgggcaaa tgttgctgta gtaggtggtg acctggaaat cgacgttatc
     1321 gcacggatat aacggcagcg acagcgccgg tccgttacac ggtacggtga gtgagtggtt
     1381 gccgtaaggg ccagtgttgg cgcggaaatc catcttcact gccgtcaggc gaccatcttt
     1441 tttcgctccc agtttgacgg tgactttcgc gacgtgacga gaggtgttag caataaactc
     1501 ttcttcacgg gtgtagcgga acagtaccgg acgcccggtc acgcaggttg cccatgcgca
     1561 cacttcttcc agcaggatgt cctgtttgga accaaaaccg ccgccaactc gctctttaat
     1621 gacatgaact ttatgctgtt tcatgcccac gaggcgcgcg acctggcggc gtaagtgcca
     1681 tggtacctgg gtggaggcgt ggataaccag acgatcgccg tccatccggg taaagcagat
     1741 atgtgtttca gtcgggcact gctgcgcctg cgttgagtta taggttcgct caatgatcac
     1801 atcggcatcg gcaaagcctt tgtccatatc gccaatatga ccatgaatac tggcggcgat
     1861 atttttgcga gggcgagaac cgatcgggaa gttgatgatc atatgctcgc cgcgctgggc
     1921 tgcatggctg ttatcgtctt ccagagtatc tggcgcacca gcaacataca ccaccggttc
     1981 atcgtgcacg acaggcgcat cttccgccat tgcttcgtcg atcgacatta ccggcttaag
     2041 cacttcatat tcaacgtcga tgagcttcag tgcttcgagc gcaatttctt cactttctgc
     2101 gacgaccgca gcaacacgat cgccgacgtg acgcattttc ttgccgaaca tacggcggtc
     2161 aagcggtgac ggttccggtg cgctctgacc ccccggggta tagtagatat cagggcaatt
     2221 caggtgagta ataacgtgaa cgacgcccgg taaggcttca gctttgctga catccagatg
     2281 agtaatcagt gcgtgagcgt gtgggctacg taacatttta atgacgcagg cgtcagccgt
     2341 tacgcggtct tcaacatagc agggtttcgc ctgcaccatt ttcgcggcat cagttttagg
     2401 ataatgcttg ccaatgacgt caaggtcgtc acggaaagtc ggagcgatat caatggtggc
     2461 ctgcggatta tttttacgtg caaccgccag ttcaatgacc tgatagtatt gttgccagcc
     2521 tgcatcgcgg ctgaacaaac cagaaagcgc gtcgtcgatc tcttcgcggg taggtgcggc
     2581 gatgcgatcg agaagatcgg tgataatcag agctgcagct ggatcgttat aaccagactg
     2641 caccacgcca acatcaacca ttgcctgttg aaccagactt aactcgttcc atttacccag
     2701 agattctgcg gtacgaatat ctgccttctc taactgtgcg gcaataagca aggacgcgtt
     2761 aacgatatta ccgttaaaga tgattgcgtc agaaccggca aacccgaaac catcatcact
     2821 gttgcgtaca gagtgcattc ccatgttaaa caacagcttt tgcacgtttt cgcctggatt
     2881 aacggttagc tcctgaggcg cgccatttaa agtaaagtgg atgatcatac ggcttcctcc
     2941 ccagcctgtt ggcagtcggc atacaggtcg gctaccagaa ctcccgtgat atagcgttta
     3001 taggccacgc tgccccgcaa atcttcctgc gggaaaatgg cgttggcgac agcctgttca
     3061 agtgcattgc cttccagatt ttgtttttcg acatcgtgca gacgcagtgc tttactggcg
     3121 acgccatcca gcgcaattcg cataccgtcg tggtctgtca ttgcaacggc tgccgtcacg
     3181 acggttaaac ctgcctgaga acggctaatt ttccgggtcg cacaggtgcg atacggatct
     3241 ttaatgataa tttcggttag caggcgatcg catgggcagg ccaggtagtc ctcgattgac
     3301 agcgtttcgc cgttgccaaa aaccagttca gcatccagtg ctagcaggac gggaagcagc
     3361 actgactctt cctgccgggc ggcgatttca ccaccaatgg tcgactgatt acgaacatgg
     3421 cgtgagtaaa caaaaccgag ggcttcacgc agtgctgcag gaataaatcg cgcatcacgc
     3481 agtggctgca agcgagacat tgcgccaatc cgcagtgcac cgttatccca gtcaacccag
     3541 tccagttcca gatcctgtaa ggaaatggca atctttttat cggtacgggt tggtgtagca
     3601 ttgagtttgc tgcccccggc gaaccagacg gcttcatcct ggtagcggcg cttcagttcc
     3661 agcgcctgtt cgacagagtc gggcctgaaa aattgttcaa tcatcttctt tccttattta
     3721 accctcttcc tgcgggaaga gggcatctgt catttatgcc agcgcatcca tccgacgcca
     3781 catactggca gcggcttttc tggcttgtgc ataaatggaa tcgcaatcga cgttaaactg
     3841 acggtcttca tagaccatca caccattgac catcacgctg tgaacgctgc ctgagcccat
     3901 accgaaagcg atatgcccgg cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc
     3961 gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc ccaaatttcg cgccaaagtt
     4021 gcggctcatc agttcgttgc cattagtcag ggctttggca aaactgtcag gccacagcgg
     4081 accacccgca tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc
     4141 aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta aggtgatggt tgtagccgac
     4201 atggttgttc atgtttgaac gggcgttatg caccaggaac gcatcgcgct gattgagtag
     4261 ggtgatgtca tctttcgaca agtaaagccc atgagcgacc agcgttttgc tgtcgatgag
     4321 atcgaattgc gctagtcgtg ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac
     4381 gtcgtaaagg tcttccgcag cgtgaatatg caaaccacgg cctgtggctt tcacggcttc
     4441 acgcagcatc tccagaccgg catctggcac ggtaaacggc gcgtgagcac cgatatgtgc
     4501 ttccaccaga tacggctcgg ttgctgcttt cttcgcctca tcaatctgac gggcaaagcg
     4561 gatgttttct tctacacctt cctgcaactc tttgatgccg ttgttacggt cagtagtttc
     4621 aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga
     4681 cccgccgata tacgccggag aggcatggtg atcgataacc gatgtacatc cgctcttaat
     4741 cgcttccagg gaacaaatca gcccgctgta atagagcgac tcttcatcaa gggcgcgatc
     4801 gagccgccac cagagatttt tcagcgttga gatgaaatcc gggcaggggg cgatgtttgc
     4861 cataattccg cgggaaagcc ccgagtaaaa atggttgtgc gagcagacaa ttcccggcat
     4921 cacaatccga ccatgcatct ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc
     4981 gccgatagcg acaatcacgt cattttcgat agcgatatca acgccttcct gcactttcgc
     5041 cgggtgtaac tgcactgcgg tgacattctt cagaatcaac atgattacac ctccacgcgg
     5101 cccagcagat aatgatgatg ctggtggaca tggctgatga tgcggcacat atcgttcagc
     5161 tccggtggta cgttgttaaa ctgaccttcg ctgtcgatgt ttaacaccca gctttggtta
     5221 ttcagacgta ctcgtacccg gcaatcttcc acaaggaagc ctgggttgct gctgttatca
     5281 aagtcttgcg ccaggctgaa gacggtgatt ttgtctttgt atggtttacc gttccacgga
     5341 cagaactgag cgcagttgcc gcattcgtta cagtaagcgt cgaggtgcag cgtctggaaa
     5401 cggttctgga agcctgggac cgcaatggag acgttggcgc ggttcgggca gacatccacg
     5461 cacttgctgc aaacgtagtt acattcaagg cagcgagcag cttcctgcgc gacaaacgca
     5521 tcacgatcgt cactgttcac cagcgtgata gagatatcgc ctttacgttg atagatttcc
     5581 gccggattga cgttgttcca gtatttatcg ttctggtggg aacggatatt ttcccgacta
     5641 aggatggcat cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga ggatggtccg
     5701 cgctgtacgt cgccgatcat aaagacgtca gtcagacgag tttcgccatt atggtcgacg
     5761 tctggccagc cgtttttgtc cagcggcacg cccatcgcat tcagggcttc agtatcctgc
     5821 tgttcaccaa tggcggtgat caggctgtct acatgcagtg ttactgtttc gttggtttca
     5881 accggacgac gacgaccttt ctcatccggt tcgccaagcg acataacgcg caaggttaag
     5941 gtgccatcag catcgaaacg ttccggatta ttcaggaaac ggaactccac gccgtcgtgc
     6001 aacgcttctt catactcttc gcgccatgcg ggcatctctt gcagtgaacg acggtaaacg
     6061 atcgttgctt tttttacgcc tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg
     6121 ttacccgccc cgacaacgac cacatgtttg cccagcttga gcgctgtacc cttgttgtat
     6181 tcacgcagga aggggagtga tttccagaca ttttggttgt cgcccgccag tttcacaccg
     6241 ctatttttat cagtgccggt ggcaatcaga acatagtgga agccctgatt ttttaactgt
     6301 tcaacggtca aatcgggtga gcagccatac tcaaatttca cgccgtgagc ggcaacaaaa
     6361 tcgatatcgt gctgaattaa ctctgcagga atacggaact gagggatgat atttttcacc
     6421 acgccgcccg cattggcttc gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg
     6481 aagtaacctg cagccagacc cgccggacct gcaccaatca cggcaaccgg atggcgtgaa
     6541 ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat cccaaccttt ttccagcgcg
     6601 acttttttca gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt acagttgtat
     6661 tggcactggt gatcgcaaat atgaccggta atggcgggca gagcgttgcg ttggtagatg
     6721 agttccagcg cgtcggcata gcggtgttcg ccaagcagac ggatgtattc cggaatatct
     6781 tgcttgatag cgcaggcagt aacacagggg gcaacgtagc agtcggtcag cggcaggtct
     6841 tctgccactt caatacgctc ttctggcttc cagtgtttct gggtgtattc catggttaac
     6901 gcatctgctg ccagtctgtt cagtcgttcg acgtcaacat ggtcaagccc ccaggcgtcg
     6961 gagccttcca gctcgcgcat gcaggcactt aagcgcaaat agccgccagg tttcagcagg
     7021 tcggttgcca tagtaatagg gcgaatacct gtatcaaaaa tatcgcggat agtcagctga
     7081 ctggcaccac cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct
     7141 gcaacattga tggagagcgg gaacagcgca cggcctgaca tatacatctc ttcaccaggc
     7201 agtgcgcctt tattgttgat ggtgccgaga gtgttagtca gttttacgcc aaagccgagt
     7261 gatttttctt ttgccagtgc catcaggcgt tccagcattt ccagtgcttg cgtcagcttg
     7321 aggtcgtgat caaatgactc ttcttttaag cctatgtaac cgaaaccgca gacatcgagg
     7381 atctcacgaa cacgcgcgta ccccagtaag gtcgggttga gtttcacaaa ggtgttgagc
     7441 cctttttctt ccagcatgta gcggcaaatg gcttcgattt catgcggagg acagccgtgc
     7501 atggtggaga gggtgacgcc ctgcaccata ctggtgggga tgcgagcggg taaggcttgc
     7561 aagctttcgc gtttttcctg caatccgtgg cgggctaaaa atgcgtcatc ctggagtaat
     7621 ttattcagcg tatcgcgata ttgagcgaat ttcggatggt cagatgcgtc catcatattg
     7681 tcgatgaact gttgcatcgg cggttgctta ataccttcga ggttgtaacc gacgctcata
     7741 ttaaagatga acgatttacc ggaatcagaa ggctggaaca tcgcttcgag aaggtgcagg
     7801 gcaaaccagg ctttgaggta ttcatcccag gctttaagca gggtaaattc ggtagaccat
     7861 tcggtgttaa aacactcgtc ttcggcatcg atacagggct tttccagctc caggcggtca
     7921 agaatttgga cggtttttag ttcgatgaag cgtccgccag tcagccagga agtgacaata
     7981 ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga cgggagtggc acaggtttca
     8041 ccgaagacgc taacagtttt accttttacg ggtgagtaaa actgttgctc gggaatacca
     8101 aagattgagc gttgttgttg gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga
     8161 atgggacgca taatatcccc cataaccctc tccttagctt atacagatat cattgagtga
     8221 ccggtgttga gaaatccggt ggtttagggg gatagagcaa caatcgcacc actcttgcca
     8281 ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa acttaagggc tttttgtgaa
     8341 tgaactcaca ggtgccaaat gagaattaaa atcactcctg aaaattatta ttgccagccc
     8401 atttatcaat ttttctcttt tctcaaaatc agtgattcct tatcattatg ataatgagtg
     8461 ccggaaggat taaatatttg aacgcaatcg tttaactacg ttttctaaat ttcagtatac
     8521 ctttccttcg ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat ttctgcattt
     8581 tctatttcga cggaataatg atctcccata aggagtgcct gacgcatatt ggtgacattg
     8641 ggtcgttgga ttgcctgtat caagcatggt tttgacacta aaatcggatg gccggggatg
     8701 ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt tctaaaaata
     8761 tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg
     8821 ggtactgcgg ccttgacgga tgttagtaaa ccctgcgcat aatctgggtt gtgaataata
     8881 gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata gccggtgact
     8941 aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag aattgttccc
     9001 tgttcccagg gtaacatcat tttccattgc cccattcttg atgataatcc ggcagcggta
     9061 attatacagt cgatggctga catagtggtt ccattattta taggggctaa taacgcctga
     9121 taattgcgtt acttcctgat ggattaagtt ggggataatt tatcccagag aggtcataaa
     9181 gactcgtgaa aagtatagtt gacccatcgg cattagtcat tgatttaggt gcgcagaaac
     9241 gtcccactgt gatttctgtt gttggtgctg gggggaaaac cagcttgctt ttttggctgg
     9301 cagaactgct ccaggctagc ggcaggcgtg tgttaatcac gacgacgaca catatgttta
     9361 tgccaacgtc tcactggccc gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt
     9421 ctcttacatc ccccatttca ttttgttttc acagctggaa agcgaaccag ggaaaagtgc
     9481 agggatttac gccagaagcg attgatgcac tggtgcaacg accagagtgt gacgtaattc
     9541 tcattgaagc agatggctcg cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt
     9601 gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg aggacatact ttgggcgcga
     9661 aagtgagcac agaaaatgtc catcgctggt cgcagtttgc tgatattacc gggttaacac
     9721 ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt tcgccaccct cagggggcgt
     9781 ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt tctcaatgtg
     9841 agaatgcgat tgcacaaagc gagctccttc aaccgctgca acaacacgac gtagaggcaa
     9901 tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg aattagcggc
     9961 ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata ttttcacaga
    10021 ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg
    10081 aggctcgact ccccgccatt ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg
    10141 tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcacttc aggcattgca
    10201 ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg atgctgtcgg
    10261 gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc catggtatgc gtccacgtct
    10321 ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg
    10381 atttgatatt gccgttgccg atatttatcg cgaaagcctc aatcctgaac tattccctcc
    10441 atcaaccacg cttctccatg ctgagtcgtt tggtgcagca gtggaagcac tggatattcg
    10501 ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc tcgacaaact
    10561 cattgaacag cccattgcat ggttggggtt gctggcaagt cgccgcaagg ttcagctttt
    10621 cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc
    10681 cgttggttac aacataggtg cggaaacgcc gcaggagatc gccatcagcg tgctggcaga
    10741 aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaaacctt ctcatccttc
    10801 cggacaccag ctggtggtga ttcgcggtgc gggagatatc gccagtggtg tggcgctacg
    10861 tctgtatcat gcgggtttta aagtgatcat gctggaagtg gaaaaaccga cagtgattcg
    10921 ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac
    10981 tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt accgaacgcg gattcatccc
    11041 tgtgatggta gatcccgcct gttcattgct tgatgaactg aaaccgcttt gcgtggtgga
    11101 cgctattctg gcgaaacaga atttgggaac gcgggcagat atggcaccag taacaatcgc
    11161 gcttgggccg ggctttactg cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg
    11221 gcactggctc ggtcaggtga tttactctgg ttgtgcgcag gagaataccg gtgttcctgg
    11281 caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag gcattatgcg
    11341 atccaacgtg aaattaggcg atctggtgaa agagggcgat gtgattgcct ggattggtga
    11401 gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg acggcctggc
    11461 agtggtcggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaacgg ctgatttcac
    11521 cagcgtttct gataaagccc gggcgattgg cggcggcgta cttgaggcgt taatgatgtt
    11581 gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc
    11641 cggatggtat tacccacccg gccttaaaat tactgacaaa tcaccgttcc ggttttgcct
    11701 tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag
    11761 cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc aaaatgacct
    11821 tcttcgataa agcgttcagc atcacttaat gacaggcgat cgagccactg ttcattctct
    11881 ttaccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat atcggcatca
    11941 atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcgct ggcaccacgc
    12001 agatggttac cttcacgaat aacagggata ccaccaccgc caacggtgat caccacctgg
    12061 cctgcatcta ccagagcttt aaccgtttct ttttcaatga tatcaaccgg ttttggcgag
    12121 gcaacaacac ggcgatagcc gcgaccagca tcttctttca gagtgtaacc ctgttttgtc
    12181 agttgctctg cttcttgctc ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat
    12241 gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg ttgctacagg tttattaatg
    12301 ccccgagaaa gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg
    12361 cttaaggcaa cacagacaga catcggcagc attggcgagt gtgcttcagt ttttgcggca
    12421 gcttcaaagg cctggttaat catgcccacc tgtggcccgt taccgtgagt gacgatgacc
    12481 tcatgtccct gggcaattaa atcaacaatt gcctgagaag taatttttac cgctttcatt
    12541 tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat tttcttactc
    12601 atttttcctc actcatcatt cttgattgtg tatagatccc tgacagtggt tagagcacgg
    12661 gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc
    12721 cgtcacagaa aatcgtttca ccacgggata aggttctgac aatcgcgccc tgacaggtaa
    12781 aaccctccca tggcgagtag tcggcgttgt cgtggagatg gcgatgctga atttgttggc
    12841 tctgacgtgg gtcgataatc accacgtcgc catcggaacc gggcgctaat aatccttttt
    12901 gcggccacag gccaaacagc ctggcgggca ttgcgctggt taattcaaca aagcgttccg
    12961 gtgttatacg tcccgtcatc acgccactgg aaaacagtaa ctgcatgcgg ttttccacgc
    13021 cgggtaagcc atttgggcag cgactgaaat cgcctttaga aatttgcagg cgttgagcca
    13081 ttgagaaggt gcagtgatcg gtcgccacca cgtcaatcgc accatcgctg atgccacacc
    13141 acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac ttcatgccgt
    13201 cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt tcaacccaga
    13261 ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg ttagacaggt
    13321 gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg
    13381 cttccgcttc gcattccaga gggcgactca aggcatgata gcgcggcgcg gttaaccctg
    13441 cggcgataaa ttccgcccgc ttgctggcga tagccgcatc attttccggg tgcacggtgg
    13501 tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa aacctcgtca tcgttgagtt
    13561 tgtattgata ggttaaatag agtttaaaac tgctcagtcc ttcctcgacc atcatcggga
    13621 tttcgtcgag gattgcgtga ttaatgtgct ggatcacacc gtgaaagctg taatcgatga
    13681 ccgctttatg ggcggcataa ccacgataaa cctccagttg atggcgtaac cgacagccgt
    13741 ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca gctgcgcggg
    13801 taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg aaatgcgtat
    13861 ggacatccac gccgccaggg aaaacgtaac agccagtggc atcaatttct tcatacggga
    13921 gctgcggcga aatattgttg cccaactggc gaacaatccc gctttcaatc agcaaatcct
    13981 gtttggcttg tccatctgcg ttaacgacag tgccgttttt gatcaatacg cgcatagcaa
    14041 actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta ctctgttgcc
    14101 agccagctta acgggattgc agcgtacatc gcggcacagg tcaccaggtg agatttccag
    14161 gttttttcgt taggtgcatg cgcttcaggt tctttacccg ggccaaagcc gatcaccgga
    14221 atgccgtgac ggcccatgat agatacgccg ttagttgaga aggtccactt atcaacaacc
    14281 ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct
    14341 tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc agtccaggac
    14401 ggacggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc aggcagggcg
    14461 cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacggcg gtcaatagag
    14521 acggcgcagc tatctgctac agcgcaacgg cttggggatg tgaagaagat ttcagaaacg
    14581 gtgagggtgc ctttgccgag gaattcgtca taacccagac gttgggagag ttcttgtaat
    14641 tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg
    14701 tggcagctaa caccctgaac atcaatacga atttccatac gaccgcgctg accacggtat
    14761 atctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc ggattgttca
    14821 ataatgtact gccagcacag accgtcgcag tcttcttcct gcacagtacc ggtaaccagc
    14881 agggtatatt catcttccag accgaggtct ttaatgattt taccggcata aaccatagat
    14941 gccatgccgc cttcctggtc ggaagtaccg cgaccaccga ttagctcatc agtttccatg
    15001 ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa tgccgacggt atcgatatga
    15061 gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt gcccatcggg
    15121 tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg atgtactacg
    15181 cgtttttcgt cgcagctttc actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg
    15241 tccgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt cttagccatt
    15301 atttttctcc agatttttta ctccccgttt tgcagggagt agttccaata gggtgattaa
    15361 ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg
    15421 ccttcagtgc tgataaccag caccacggca tctttgttca gcgccagttt ttccatcagg
    15481 ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac agcgccggat
    15541 tcaccggaga tgatgcgcgg gtcgttgccg tacggattac ccagcacgcg catacctaat
    15601 gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag gatttcccag
    15661 cccagcgggt taggttcgcc acaggccagg cccgccatga tggtggccat atcaccgcca
    15721 acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt gtcaggttca
    15781 acaataatgc tgtgcagatt ttgcgggcta tagacgtcga ccagataacc cagcacacca
    15841 ccggccattg ctccgacacc ggcttgcagc agaacgtgcg tcggggttac gcccatttca
    15901 cgcatttgct cgacggcttc atctgccagg gttgcgtagc cttgcatgat ccaggttggg
    15961 attttggtgt aaccttccca tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg
    16021 tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ccgtgacgat gcactcggca
    16081 ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc
    16141 gcattctgtc cgagttgctg cgctgcccac gccacaccgc gcccgtggtt gccgtcggtg
    16201 gtggtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt
    16261 tcgatatcaa gatgatattt ttcacacaat aattgagcga tggcgtacgc accgccgagc
    16321 attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacaagaat tttcttcaca
    16381 ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg ttgggcgata accagctatt
    16441 ttctggtgga actggcgcgc cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg
    16501 ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga catatcctat
    16561 ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg ttatttcacg
    16621 cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc gtatttacgg
    16681 gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta ggttgcgata
    16741 cggtattttt cgaatacgcc ttcagtcact tcaccttctt tacaggatac gccgctgata
    16801 tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc catcatctct
    16861 tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct
    16921 tttaagcctt cgtgatcgtt agcgcgcagc aattcggtac gctgttccat cactttgtaa
    16981 ggtgcccatg acttcggata aacgatgtct gcatctttga acgcttcttc catgctggtg
    17041 acctgacgga agctaccgcc agatgcttta gcattgtttt tcgccacttc aaccacatcc
    17101 gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa gcgagtcatc
    17161 agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttggaga gtaggcccag
    17221 gtcatggcga tttttttacc tttcaggttt tccagtgaac caaagtgttc acgtaaccac
    17281 gcgaggtcag ccattgactg agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc
    17341 ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc
    17401 atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccaatagc gtcggcgcag
    17461 aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct
    17521 tcgtcgagat cttgttgtgc aaggccgagc aggttaagcg cggaagcata agagaagcgg
    17581 gtacgggtgg agttgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt
    17641 gagatgtttt cagcacgcag tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt
    17701 tcatctggcg tctgttccca cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc
    17761 gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg gtagtggaat
    17821 gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt gattggtgtt ttcacagcta
    17881 tacctgggac taacaataag tgtgccagaa ttatccacgg ggctgcaaaa gactaatttg
    17941 acggataatg tgtggggttg atcacaaatt gatgccagct tccggttgtt tgtaaaatac
    18001 gaaaacgttt ggcttttttt atcgcgttat ttatttgctg ctaagtatca gaaataagat
    18061 aaagggacag gctatctctc ttcagtttga taaactcacc gccgtaattc tcatattgat
    18121 atagaaatgt gatatctctt ccataatttc attagaaaat gataaaaatc ttatgttttt
    18181 atcaaagcca atttcgcctg agttaatatc atcatgatct gtgaaccatc aacgtcttca
    18241 atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac tacgcagtca
    18301 gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg
    18361 cagcttgagg ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac gggcgcgtat
    18421 gggaagtttc ttggtcgcca gttgagcggc aactcacgcc tgctccgcca cgtcctggaa
    18481 acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgcga aggttgcgat
    18541 agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt
    18601 tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagagca tatcagtgat
    18661 aatttacgcg aattttctga ttatgttcgc catatatcca ccatttttgt ttcgaaactt
    18721 ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat gatcgataat
    18781 atggatcagg gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt taatcagact
    18841 gccttaaaaa cacttggtgt tgtacaaaat aatattattg ggaaacctat ccgtttcaga
    18901 ccattaacat ttgagagtaa ttttactcat ggacatatgc agcatattgt ttcgtgggac
    18961 gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca attattttta
    19021 atggcatttc accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccacat
    19081 attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt
    19141 attgcaccca gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtc
    19201 gtcgcccgag caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac
    19261 tgtgccgcga ttccggagca gcttctggaa agcgaactgt tcggttatgt taaaggcgca
    19321 tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg
    19381 ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct
    19441 attgaggcgc gtgaaattct gccgattggt gccagtagcc caatacaagt cgacattcgc
    19501 atcatttctg caactaatca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa
    19561 gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg tgaacgtcag
    19621 gaagatattg aactattggt gcattacttt ttacatctgc atacccgtcg tctgggatcg
    19681 gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga
    19741 aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgtagttcc ttcaggtgaa
    19801 gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt
    19861 gatgtaacag aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg
    19921 gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaagcaa
    19981 gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag
    20041 ttgttaaaca cataacctca gtgaatccgg cctgaattca ggccggatat ccttttactt
    20101 cgttttctcg caatcctgaa ctgtatttac aatcatctga taccccgtgc agcgacaaag
    20161 atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc gtggtttcgc
    20221 cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact gcactgcgcc
    20281 ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt caccttccag
    20341 cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt ctattgctgt
    20401 gccgtcgacc aacaccgtac aggcaccaca ttcacccacg cagcaccctt gtttgacact
    20461 tagcagtcct tgttcgcgga gtaattccga gagtggcgtg cctggtgcgg cgtgaagctg
    20521 aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat
    20581 tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga
    20641 tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca
    20701 gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg
    20761 gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg
    20821 aaattgccgt tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat atccatcgcg
    20881 tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa
    20941 tgaaaggcga cgaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag
    21001 ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga tctccagttt cgcgtcataa
    21061 attagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag
    21121 gtagcgacgt tacggatctg cggtccagca atggacgtgg ccgcagcaca taacgccggg
    21181 agatgacgtt gagttatagg atcttctatt agctgggtaa atgtcgttgc agagccgata
    21241 cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt atgaatatca
    21301 acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc agtgccaccg
    21361 gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc aagggttgct
    21421 gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg
    21481 gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa caccggtagc
    21541 catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct cacccagtga
    21601 cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg
    21661 cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct
    21721 tttagcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc ccattccgcc
    21781 gtgtacctga ccttctgcca gcagtggatt aagaatatgc cctgaatcat gaacgttgag
    21841 gatgcggttg atgatgactt tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca
    21901 gccaaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga gctgcccgcc
    21961 gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc
    22021 cggtcgttca accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg
    22081 tagcatgact gcggcgtgag cgatgatttt ctcttttaat aatagtgccg cactgcgcag
    22141 cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac
    22201 gtcggtatct tgagttgaaa taacgcgaac gtcgctgacc ggaaccccca cggtttctgc
    22261 caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac
    22321 gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta cgccgacagg
    22381 ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca aattgccttg
    22441 ctggttctgg cattctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag
    22501 acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag catctccttc
    22561 gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcgtc
    22621 atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc
    22681 agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata
    22741 aaggtaagcg actttattcc cccccgcaga agcgatggag tgcccgtgag atgcataagc
    22801 gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc
    22861 catttgcccg tcaatggtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg
    22921 gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg
    22981 ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaacggtt tgatgactcg
    23041 tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat
    23101 ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca gcgatgttac
    23161 gctttccatg tgacaatgtt gaataacggg ggtctgatag tgcccctgta cctggtagtc
    23221 ggcggcatcg attgtttgtt ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt
    23281 gccaccgtta tggattggtg cagcgtcttc tgccagcgcc gcttctggcg tggtgataac
    23341 gggtaattct tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc
    23401 gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag ttagcagtgc
    23461 gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag cagtagcaaa
    23521 tggaatatca ggcacatctt cccaggtaaa aatcgccagt acgcccggca aacttctggc
    23581 ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta catatttcgc
    23641 ataacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc
    23701 aatggcatcg acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga
    23761 aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag caggcaagat
    23821 ttacgccaga aacgacgata agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa
    23881 cacagatatg atacgaaaca ggactctgaa taggggcagt gatacgtatc aatatgagtg
    23941 gcgataaaat caatgtgata aattgggatt attacgcatc aggatgggaa atagggtaaa
    24001 aagtcaggaa gttcgatacc tctacagcga atattttgct gatgatactt ccctttgcca
    24061 aatgaaatta acctctttct ctattaccac gtttttccag aagcaagaga ttgggtatca
    24121 agaggctggc tgctatgata aggcgtcttg ttttttaagc gaggaaagat tttgagtgcg
    24181 ggacgcctga ataaaaaatc tctgggtatc gtgatgttgt tatcggttgg actgcttttg
    24241 gcgggctgtt cgggtagcaa atcatccgat acaggaacgt attccggctc cgtttacacc
    24301 gtgaaacggg gggatacgct atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa
    24361 ctggcgcgac tgaacggcat ttccccccct tacaccattg aagttggtca gaaactaaaa
    24421 ctgggtgggg cgaaaagtag cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa
    24481 accgcatcgg ttacaccgtc atcagcggta ccgaaatctt cctggccgcc agtagggcaa
    24541 cgttgttggt tatggccaac gacagggaaa gttatcatgc cgtattcgac agcagatggc
    24601 ggcaataaag ggattgatat ctcagctcca cggggtacac ctatttacgc cgcgggtgca
    24661 ggaaaggtgg tgtatgtggg caaccagctg cgtggctacg gtaatctcat catgattaaa
    24721 cacagtgaag attacattac ggcttacgcc cataatgaca cgatgctggt aaataatggg
    24781 caaagcgtga aggctgggca aaaaatcgcc actatgggga gcacggatgc ggcatctgtt
    24841 cgcctgcatt tccagattcg ttaccgtgca acggcaattg atccgctacg ttacttgccg
    24901 ccgcagggca gcaagccaaa atgctgatgg cgaattaatc agcagtcagc agcgcggcac
    24961 ttgctaagga gagcgtaagg tttataatgc cttacgcatc tcgaagcggg cgtagttcaa
    25021 tggtagaacg agagcttccc aagctctata cgagggttcg attcccttcg cccgctccaa
    25081 tttatcttca cctgatcaat ctgccatttt tgctgtatct gattttctat attatcttaa
    25141 ttcaatcgct tcagactttt cgcaatcgcc gtaatacttc cccattttgt tgtaaataaa
    25201 aagcgtttac gcaatgcaat cctt
//
LOCUS       DAPPNJ010000055        24827 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000055 DAPPNJ010000000
VERSION     DAPPNJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.055"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            25..1572
                     /locus_tag="RPM73_001833"
     CDS             25..1572
                     /locus_tag="RPM73_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520203.1"
                     /translation="MLENTVDLRVSWKDGSNLKLLLPKPVSGGRFVTNDGSVHFDGVA
                     SIAHLHGIDAELLTISCAGRGYLNIELLDENPVAEKFRYLHADLPLLSGRNDKLQQIS
                     LYENYNLLNSMLACAWNSNSTLCVDFYSDRFGKDKATLNIKRYDGSFIEHDQGLLVDI
                     KNSVVFPANRIDELVVDAISLKDPGLHISLLKKDEFAYDLSALNVQDSPWLIVGKLDD
                     TARIAPVIKWGLPVLQTNDLLLNALCEADSEQRKKYFNELIFEIDNNPLQNYCCLLTE
                     YIKKYKMNNGLSLLDLDLFRGISSNYRVVVQLLISSCLSGDSDTIYDIQDELPFSWGW
                     IPVSIWKDVFQKCWAYLEKQINDKTLALHILQPFIAFMNHRAHIDRRLTPIANMLLTY
                     GELLPAGCNVLPTVSREQFNEAKQMLLRNPDSFGRISIFPKELWSSAITPELKSVFNK
                     LWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYF
                     EQAGVCH"
     gene            1572..7886
                     /locus_tag="RPM73_001834"
     CDS             1572..7886
                     /locus_tag="RPM73_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX6520204.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEVQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEVRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIAIDGEKYIISLEETTVGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANINEPSA
                     KLSPAHCALLESFITQFSILPLKNSIDALKKAMVANEELSPADIQALKIIEPMVSSLS
                     PDHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTRP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQNQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGHVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSMEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYVIAG"
     gene            8110..10968
                     /locus_tag="RPM73_001835"
     CDS             8110..10968
                     /locus_tag="RPM73_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX6520205.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKYTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10971..15887
                     /locus_tag="RPM73_001836"
     CDS             10971..15887
                     /locus_tag="RPM73_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDX6520206.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAKENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKRDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYHGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEIGKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEDGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLAGQKANLYKCFLPLGWRLGAQKGVVGFLHPEGIYEDPKGGKLRGA
                     VYPRLRAHLQFQNQHMLFPIGDRVKFSINIYTAFVSDHIGFISIANLFSAKTIELCLS
                     HDGTGEVGGIKDDETDSWNESGHQSRILKVSLERLSLFANLYDELGIPAIEARLPALH
                     ADHLVSVLEKFAKQPTRLSGLEKDYYSTQHWNEVNAQDDGIIRRETQFPEDASQWILS
                     GPHFYVGTPLYKTPREICTEKGHYDCLDLLTLPDDYLPRTNYIPACDAQEYAKRTPRV
                     SWKEQDEDEPRKVTDYYRFVNREMIGPSSERTLITTIVPPGVSHINTCLGTAFKDTQL
                     LLDYFSMTLSVPLDYRVKSTGMGHANTSLINQLPVLTNDAYRTALHSRALCLVSLVRS
                     YADLWQSCYTPDFNTQRWSRNLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDV
                     LVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARK
                     ADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLQTGTVSVTFDDYTRSDEGERRTVT
                     WQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            15887..22228
                     /locus_tag="RPM73_001837"
     CDS             15887..22228
                     /locus_tag="RPM73_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX6520207.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     SQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQDYAIAYSQYPGPEVASALDPQGY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPICLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDIERFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNMATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     CKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNHDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSDNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQKMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APDWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDITFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            22225..24339
                     /locus_tag="RPM73_001838"
     CDS             22225..24339
                     /locus_tag="RPM73_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDX6520208.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAPETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGIPSPLLVAD"
     gene            24535..24807
                     /locus_tag="RPM73_001839"
                     /pseudo
     CDS             24535..24807
                     /locus_tag="RPM73_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         6340 a         5817 c         6573 g         6097 t
ORIGIN      
        1 gctatttatt ataattcatc gctgatgctg gaaaataccg ttgatttacg ggtctcctgg
       61 aaagatggtt ctaatcttaa actattatta cctaagccgg ttagcggtgg ccgatttgta
      121 actaatgatg gttctgttca ttttgatggt gtggcatcta tagcacattt gcatggaata
      181 gatgctgagt tattaaccat atcatgtgct ggaagaggat atcttaatat cgagttattg
      241 gatgaaaatc cagtagctga aaaattccgt tatttacatg ccgaccttcc tcttttatca
      301 ggacgcaatg acaaattaca acaaatttca ctttatgaga actataattt gctaaattct
      361 atgctggcat gtgcatggaa cagcaatagt acattatgtg ttgattttta ctctgacaga
      421 tttggaaagg ataaagcaac actcaatatt aaacgctacg atggtagttt tattgaacac
      481 gatcaagggt tactggttga tataaaaaat tctgttgttt ttcctgcaaa tagaatagac
      541 gagctggttg ttgatgctat ttctcttaag gatcctggct tacacatatc gttgctaaaa
      601 aaagatgaat ttgcttatga tctttcagct ctgaatgttc aggatagccc atggttaatt
      661 gtggggaaac ttgatgatac agctcgcatt gcaccagtaa ttaaatgggg gttacctgta
      721 ttacagacaa atgatttatt acttaatgct ctatgcgaag cggattccga acagcgaaaa
      781 aaatatttta atgagctaat ttttgaaata gataacaatc ctttgcaaaa ttattgctgt
      841 ttattaacgg agtatattaa gaaatacaaa atgaataatg gcttatcctt gctggatctg
      901 gatttgttca gaggtatttc gagtaattac cgcgtagttg ttcagttgtt aatatcatca
      961 tgtctttctg gtgatagcga tacgatttat gatatacagg acgaattacc cttttcatgg
     1021 ggatggattc ccgtttcaat ctggaaagat gtttttcaaa agtgttgggc ttatctggaa
     1081 aaacagatta acgataaaac attagcatta catatattgc aaccctttat tgcttttatg
     1141 aaccatcgtg cacatatcga tcgtcgtctg actcccattg cgaatatgtt acttacatat
     1201 ggcgaactcc taccagccgg ttgtaatgtc ttgccaactg ttagtcgtga gcagtttaat
     1261 gaagctaaac agatgctatt aaggaatccc gacagctttg ggcgtatcag tatcttccct
     1321 aaagaacttt ggtctagtgc tattactcca gagttaaaat ctgtttttaa taagctttgg
     1381 attgaagata aatatcactc acggcttgaa aaacgtttta atttgatgtt agtcgcagcg
     1441 ctgttaaccc aaaaagataa taacttgata catcaattgt ctgcgctttt tgaattccac
     1501 tatcagcaag ccccgcagca attaggggta atctatcaat attattttga acaagcagga
     1561 gtatgtcatt gatggacgtt cagcaactgc attatgcgcc acttctaaat cagcttcatc
     1621 agcgcgcggt gaggtcggtg gtaagtcagt tggcattgcg gagcaagtcg ttgactcatt
     1681 atctggaatc acaatatttc cagcctcccg gtcaggaggg gtcactgctt gcagacccgg
     1741 tattcgaaag cacgttcggc tggatgcctg cggatgaaac aatggaggat ttgcgggaca
     1801 agctaatttc cagcccgctg gttgatgcat tgagcaaagc gccggtgcca tttcgtcatc
     1861 aacttacggc gtggcgaacg attctggaag acaaaaaatc cttgttagtg agcagcggta
     1921 cgggatccgg gaaaacagaa tgctttatgg taccggttct ggaagatctt attcgtcagc
     1981 aaaaatcgac ccgtcagtca ctaacaggga cacaagctat tttcttgtat ccgttaaatg
     2041 cattaattaa cagccaacag gagcggcttc gcgaatggac gcgcggattt aaggggaaaa
     2101 tacgcttcgc gctatataat ggtgaaaccc ggcataccaa atacgaagtt caggaagatc
     2161 agttgaaagt acctgaacaa gctttgagtc gggaagccat ttacgaagca ccaccttcca
     2221 tcatggtgac gaataccacc atgctggaat atatgctgat tcgtcgtaaa gatgccccaa
     2281 tcatcgaaaa gtctcagggg atgttaaagt atatcgtcct ggatgaagcc catagttata
     2341 ttggttctca ggctgctgag ttagcgctgc ttttacgtcg tgttatgcag gctttcaatg
     2401 ttggacctgg cacagataaa cctgtgcaga ttatcgcgac ctctgccact attggtgaag
     2461 actctcctga aggtaataag gagcttgcta aatttgtcgc tgatcttgcg ggcgttacag
     2521 aacgtgatgt caaagtggtt cgcggctatc gacaaatacc gcgggtatcg gagtcgctga
     2581 ttcgtcatga atatcccaca tctttaactt ctcttaaatc tttgagccct caggatcttt
     2641 accaacagct ttgtcattac cgtgtggccc agcaattgcg tcaggcgtta actcatcccg
     2701 gacggcaggc agtacgtttg agtgagttgc tcaatgttgc cagaagaacc tggccagata
     2761 ttaatcaccg tgagttactg caactcctcg acctaatggc gagatcgcga gaaggtgaac
     2821 tggcttttac gccattacgt atgcatgggt ttatcagaac actggcgggt ctatgggcat
     2881 gttcaaacaa acaatgctca cataaagcgc atgaattaaa tcagagtgac tggccatttg
     2941 gtcaggtatg gtttgagcag cgccaatatt gtgactgtgg tgcgccggta tttgaagttt
     3001 tacgttgctc cgggtgtggt tcagcttatt tgtctgccaa agaggaggtg cgcggtgatg
     3061 gaacgaactg gttggtagcg caacccgctg ctgccgaagt ggatgaattt gctcttgatg
     3121 tggatgttta ttctgaagat gaagacaacg atgaattaga caataactct caatttgatc
     3181 gtctgattgc gatcgatggt gaaaaatata tcatttcact ggaagaaacg acggtgggga
     3241 aaattgatag tgaagcgcaa aagcactatg aaataaacct gttaagaccg gagtctcgag
     3301 gtgagggacg taataattcc tttgcttgtg tttgctgtgg tgacacccaa cgaaaaaata
     3361 atcctttatt ccgtccgctt cgacttggtg cgcccttttt tttaaatgaa attattccta
     3421 ctctgctgga gttttcacca ttaccgcaga caagagaaga gcagttagga ccgtttaatg
     3481 gtcgacgtct gctcaccttt acggattcac gtcagggcac tgcccgcatt tctgcccgct
     3541 tacagcagga tgccgataaa gcgacgttgc gagctctttg ttaccaggag ttggcaaata
     3601 ttaatgagcc atcagcaaag ctcagcccgg cacactgcgc attgttagag agttttatta
     3661 cccagttttc tatccttcct ttaaaaaact ctattgacgc gctgaaaaaa gcgatggttg
     3721 ctaatgaaga gcttagcccg gcggacattc aggcacttaa aattatcgag ccgatggtga
     3781 gttccttaag cccggatcat gctgaagcga tgcaaatact ccaggctaac tctggcaatc
     3841 agcagaagaa aaccatgtca tggcaaacac tgtctgaaca gctggttggc agtgtggacc
     3901 ttgccgatag aatgagaggc agttttaaag aattgtctgg gcttgatctt actaaacaac
     3961 agtttgcaga tttttgttta tacagtgagt ttggtcgccg ccctaaaaat gcctggaccc
     4021 tcgaaagtct gggtctggta gccattcact atccatttat tgataaagtt acaacctgcc
     4081 cgcaggactg gaagacatta ttacccgatc ccgataagca attagcggaa tggcgtaatt
     4141 tattgaagat tactcttgat ttctttatac gtgaaaacag tgccgttttt tacgaaaacg
     4201 agcaataccc tcgctggatg ggggctcgtt tcccggtaaa aatgctccag ggtccagata
     4261 aaaaaaacaa agggcagaaa cgtgatcaac tttggccaca aatacgcgat cgccattata
     4321 accgggtcat taagctatta attgcagtct ttccggcaat acaacctgag caggctcact
     4381 ggaaatcgtt agttaaccac ttaatgattg aggtctggga tgcaattcga ccctgcttac
     4441 gtcagtttga gtcgggttat cagctggata ttaaacaaca agctgaattt tacagtccaa
     4501 agcaagtgtg gcgttgcccc tatactcgac gtgcgttaga cgtcacgtta ctgggatact
     4561 ctccgtattt accgggtagc aaagaaattg cacctgaaaa agctgtgttg attgaaatgc
     4621 ctgagcttcc tgtacgtcac tggcgtttgt ctggtggtgg agaaatcgcc cgagaagaac
     4681 gtctggagtg gctggaaagt aatgcgctga ttcaacatgc ccgcgaggaa ggattatggt
     4741 ctactcgcag cgaccgactg gccctgaaag atagctggta tcgtctggag gaacactctg
     4801 cgcagagaac gcctgagcag aaccagttta atgaaaaaca atttaagtca ggcaaagtta
     4861 atgtgctgaa ctgctccacg accatggaga tgggggtgga tattggtggt atgagtttgg
     4921 tggcaatgaa caacgttcct ccagctccgg caaattattt gcaacgtgca ggtcgcgcgg
     4981 ggcgtcgtgg agagagtgca tctgctgcca ttacgttatg taaaaacacg gcgcatggaa
     5041 tggaggtatt caaagatccc ctctgggcgt tcaacactac tgccagtgcg ccacgggttc
     5101 gttttgggag cagtagtatt gttcaacgac atgttaatgc cctggtttta ggtctgtttt
     5161 tacgagctga agttcctgat gcgacaaaac tgagttgcaa atggttcttt gaaggtgatg
     5221 agagccagtg tcttcgtttt ctccattggt taaatcacca ggccgatcag ctggcggata
     5281 aacttaaacg ccttactcag ggaaccgtcc tgatgtcatt gacggcaacc cagctcttga
     5341 cccgcacaca agcaatgatg cagcaggttg atatccgctg gagaagccaa cttgcgatac
     5401 tgttggaaaa tatcgaagcg ttgaaagcgg ataacagcgc gtgggaagaa acgccagcag
     5461 gtaaagccat cgcttatcag cttcgtgatt atcgcggtgc atatctgttt tctaaactca
     5521 ttagtgaagc gttcctgccg gggcatggtt tcccggttgg cgtcgtcaat ttcaattatc
     5581 tgacggcaga tgagctggaa aaacgtcgcg caataaaagc tactcaggct gaccctaatg
     5641 aagggggaga aagcttttct cgtcgtattg aaaaattgcc cagccgtgat ttgcctacag
     5701 cattacgcga gtatgctcca ggtgcagatg tggtattggg cggaaaagta tatcgttcct
     5761 cgggcattat gcttggcaag gtacttgcca gcgggcagga gctgtcaggc gatcatcata
     5821 ttccgtggtt ctggcattgt cgtaaatgtg gtgcgggggc aaccagtact acccgtcctg
     5881 tcgaatgtag tcactgcaaa gcggatatac agcaacttga tgtgaagcgt tatttacagc
     5941 ctgttggttt tgcgacagat atccgttatc aggcgcataa tgatgtatcc atgcctgcac
     6001 aacttccctg gaaagatcca cgtgttcttg taccgtcttc ggtgtgggtt tctttacccg
     6061 atgctgggct aggacgttac cgttttagcc atagtgggga attgttccac ttcagtgaag
     6121 gggaatttgg gcacggctac gcaatttgcc tctcatgtgg tcgggcagaa tctcaaaatc
     6181 aaccccagcg tacccctgaa aatcttaaga acccagagag ggaaaatacg cattacctgt
     6241 tgcgaggggg cagtaacgat cgtcagggga gcaataaatt gtgccacggt catgtgcata
     6301 aagatctgtg gttggggtat agcagtcgta cagatatggt tgagctgcaa ctcaatgatg
     6361 ataatggcct gttgatccgt gatgaggttg ccgcgcgatc ccttgcggtc gctttgcgtg
     6421 aagggttagc gcataaattg ggtatcgaaa atactgagct gggcgtgacg acgcagcaag
     6481 cgcgcgatat caatggctat accggatatt ccatcttcat ttacgacaat aatgcgggtg
     6541 gtgcaggtta tgcagtgcaa ttgatcgacc attgggctga tgtctttaat tacgccagaa
     6601 aattgctcga ttgcagttgc gataaattct gtcaccactg cttgctcagc tacgacagtc
     6661 agcattatgt caacagatta gatcgtcacc atgcattaac cctgcttacc aatgtacggt
     6721 tgcagcgact taacctcgcg ccagaatatc agtacttcgg cgatggtagc cgggttgaaa
     6781 ctaacccgct gagtttacgt attgcgcaat gcctgaacag cgagatttat gactcctgct
     6841 cgctggttct tgcggggccg caggaacagt gggattttgc ccaatggcca ttatttaaag
     6901 agttgctcca gtttgcctcc tccggtgggc atgttgagtt gctggtggcc acgccgttgg
     6961 caaacttaac tgatagctcc agacatcagc tttccgcgtt ggcggcgatg ccgggagggc
     7021 gcttacaagt gaaatccatt gccactgcgc aactcatgca aggaaagggg cgctggcttg
     7081 cgcaggtgac tcgcgaaggg caaagccaac agtgggccgc agacgacagc gctaccgttg
     7141 cacctggtga attatgggga caaagtgcgt cctcaccagt agtgacactt aaaggaacgt
     7201 ccggaaaaac cttctcgggt cagaccttaa gtgcagaaga tttactgccc gccttaccca
     7261 ccggggcggt gcgcatcaac ctctgcgagc aactggacgg gccgctggaa gggtttggtt
     7321 cgcgtttctg gtcgttagtc acgcaacagc acgctggctg gaagcaggca tttacccgcc
     7381 acaaagagat aacgcatgtt gagtacagcg atcgttatct taatagccca ttcacggcgc
     7441 gtttgttagg tgaaatctta actgagctgg ttgaacaggg aatggcggaa cgtgccagcc
     7501 tcactgtttg cgtcaaaaaa ttggattaca attctcgcca acacgacgct ctctataacg
     7561 cgtggcttaa tgaggaagat cgtcagcagg ttgtgaccac tttacttgaa gaaggatatc
     7621 tggggccagc gtggccggga gcaattagct ggttaacggg ggataaccag agtatggagc
     7681 atggacgaga actgacagtc accttcagcg atggcagtca gcattatgtc ctgctcgaca
     7741 tggggcttag ctactggcgc tgtattgaag acaccttctt tgattttgct ctacgagtac
     7801 cgcagcaagt tgaacggctg gcaaacacgc gagcgcgagc tgttgccccg ggtaatgacc
     7861 ttaggagtta tgttatcgct ggataaataa ttgctcccct ggggatatct tccaggggag
     7921 ttgacccagc tcacttttta ataatttttg tttgaaaatc atgcgcaatt tatgcgcatt
     7981 ccctcccatt ttgtcacttt gccgggggcc ttcatgatat ctttaaggcc ctctttttta
     8041 tcgcagtgtg ccgcgccaca tttttcagga tgacgttacc gccactgccc caaatagaca
     8101 gaaaaaccga tgaataagca aaattatgca cctggtatgc gggtggttat tcgtgatgcc
     8161 gaatggcgta ttcgccgggc ggatgacagt ggtgatggtg ggtatttgct gacctgcgat
     8221 ggtatttcag agctggtgcg cggtaaagaa gggttatttc tgaccaaact ggaacaaaaa
     8281 gtagagatcc ttgatcccgc aaaaacgcat ttggtggaag atgaatccgc caattatcag
     8341 gcggcacagt tgtatattga aagtcagtta cgccaacgcg taccgacaga cagtaaagtc
     8401 cattttggac atctggcggc gatggattcc atgccgtttc agctcgaccc tacgcggatg
     8461 gcgctggcgc agccgcgcca gcggatactc attgccgacg ctgtcggtct gggtaaaacg
     8521 ctggaagcgg gtattctggt ttcggaactg atccgtcgtg ggcgcggaaa acgtattctg
     8581 gttctggcgg ttaaatcgat gctgacacaa ttccagaagg agttctggag ccgttttgcg
     8641 atcccattga cccgcctgga ttctgctggt ttacaaaaag tgcgtaaccg catccctacc
     8701 aaccacaacc cgttccacta ttttgacaag actattatct ccatcgatac gctgaaacaa
     8761 gatattgaat atcgtcacca tctggaaaat gcctggtggg atattattgt cattgatgag
     8821 gcccataacg tcgcagaacg tggaaccagt tcgttacgca gtaaactggc aaaactgctc
     8881 gccgggcgta gcgatacgct gattatgctt tcagcgacgc cgcatgatgg taaggctgaa
     8941 agttttgcca gcctgatgaa catgcttgac ccgacggcga tcgccaaccc gaaagagtat
     9001 gaatacgccg actttgccga taaaaatctg gttgtccgtc gcttcaaaaa agacgtgaaa
     9061 gatcagatgt ccggcgagtt ccccgagcgc aatattgtca aactgactcg cctggcatct
     9121 ggtgccgaag aagaagcata ccgtcgcctg gttgagagcc agttccgtga tgatgacgat
     9181 gaacaggccc agtctaacaa aggtcgtctg ttcaagataa ccctcgaaaa agcgctattt
     9241 tccagcccga tggcgtgtgc cagcgtagtg gcgaatcgtc tgaagcgtct tgagagccgt
     9301 aaagatcata atagccagag ccagatcaac gaactggagt cattgctgtt ggcgctgaat
     9361 aacattgatg ccagccagtt tagcaaatac cagttgctgc tcgacaccat ccgaaaagac
     9421 ctcgcctgga aagccaataa cacggaagat cgcctggtga tcttcaccga aagtattaaa
     9481 acgctggagt tcctcgaaca gcaactgcga gcggatctga aattgaaaga tgaccagatc
     9541 gccacgctgc gcggcgatca gggcgatacg gtattgatgg aaaccgtaga agcctttggt
     9601 aaaacgcagt cgccgttgcg tcttctggtt tgttcggatg tagcatctga agggatcaac
     9661 ctgcatcacc ttagccacaa aatgattcac tttgatattc cgtggtcgtt gatggtattc
     9721 cagcaacgta acgggcgtat tgaccgctat gggcaaaaac atcagcccca aatccgctat
     9781 ctgcttaccg aggccagcga accacagatc aatggtgata tgcgcgtact ggaagtgttg
     9841 atcaacaaag acgaacaggc gcagaagaac attggggaca gctcggagtt taccggcaaa
     9901 tatacccagg aagaggaaga agagcaggtt gcagagttca tgatgcagga tgatggtgcc
     9961 agcctgtttg atcaactgct gaacagcaac gtctcggaaa gcgccgaaca tgatctgttt
    10021 ggcgaaatat gcagtgcggt ttccagcgat gcctcaatgg ttaccgaaac agacaccagc
    10081 ttgtttgcca gcgaacaggc gtactgtgaa agagcattgg gttacctgaa agccagtggt
    10141 caaactatcc agtatgaaac cttgcctgat aataccttgt cgctggtggc accggaggag
    10201 ttacgccgcc gcttcaacca gctaccgcct gaaatcgcgc cggagaactg gcagctctat
    10261 ttaagtcagg ataaaaccgt catcaccgac gcgattgcac gcgctcgcgg tgagcaacat
    10321 gcctggcccg atgtgcagta tctctggcaa atcaacccgg tagtgcagtg gctggacgat
    10381 aaaatctctt cggcttttgg tcgtcatcag gcaccggtta tccgtctgcc atacctgctt
    10441 gaacccgatg aagatcactt cattctttcc gggttattcc cgaaccgtaa atcacatccg
    10501 atggtgaacc cgtggatagt ggtgagcttt aaccgtgaat cgctaatcgg cagtcagcca
    10561 ttcgctgagt ttttacaacg tcatccgcag ttgagtaaca agctgactaa cagtggcggt
    10621 aaagatcgta atcaccaacg ccagcaggat ttactggaag cggctattgc gcatgcccgg
    10681 gaggtattca tccatgatcg gaatgcgttt gaaacgcaca ttaaccagca actgaatgag
    10741 catctgcaaa agctggacgt tttgcgtggg cgccagttga gccaacttga gctggatttt
    10801 gccgataaca aacagcaatt gtcagtcaag cagagccgta aagagcagag acaacgcgaa
    10861 atcgaacaca attttgacag ctatatcgaa tggattgagg acaccatgac gactgaaaaa
    10921 gaaccctaca ttcaggtaat tgctgttatc accggagcgg agggttaatc atggcgctgg
    10981 tcggtattaa taacgaaaac gaattttact ctaaccatta tttgggtgag gttttcacca
    11041 gtgatatccg cgatgtgctg gaaccctgga tagccaagga aaatgcagcg cgtgaagcgg
    11101 agcgtgccgc tcgtgaacag ggcaaagacg tggagccggg ataccgcgct ccgtggaacc
    11161 agtttaacag tctggcgact gagtttttcc gcaaacttgc cgagcacgaa aaacagcgtc
    11221 agatcccgca gcgtctggcc gatcaacgta atcgctggca gccattgtta aaggcgctgg
    11281 gctacgaaat tacgccacag atccaaatgc tggatgacga tacaccgctg ccggtactgg
    11341 cgcgttacaa cagcactgac ggtagcccgt ggctgtggat tgttgaagca cacgatcagg
    11401 aagaaggaac gctggatccg ctggcgctct ccttactgac cgcgcaattc ccggcggata
    11461 ccgacaaaca taagcgcgac agcctgcgca aaaaagccaa cggtgaatat cgcagctggc
    11521 aggatctgct ctctacggcg gtcttcaccc aaaatgaacc gccgcgtttt gtgctgctgc
    11581 tcggtaaccg tcagctattg ttgttggacc gtactaagtg ggcgcaaaac cgtctgctac
    11641 gttttgattt tgaagagatt ttaagtcgtc gtgaaacgga tacgctgaaa gcgacggcag
    11701 tgttgctaca taaagattct ctgctgccgg gcagtggggc accttatctt gactcgctgg
    11761 atgacaattc gcacaaacat gcgtttggtg tttcggaaga tctgaaatat gccctgcgcg
    11821 aaagcattga gttgctgggc aacgaagcga tgcattatct gatcgaccgt ggcctggcaa
    11881 actataccgg taaccgtgcg gtggacccgg atgaactgag ccgcgaatgt ctgcgttaca
    11941 tgtaccgcct gctgttcctg ttctacattg aagcgcgccc ggagctgggt tatgcgccaa
    12001 tgaccgccaa aacctatctg caaggttaca gcctggaaac gttgcgcgat ctggagatga
    12061 tcccgctgac cagcgaagaa gatcgcaacg ggcgctactt ccacgacagc ctgaatatgc
    12121 tgtttaaact ggtgcgcgaa ggctacaacg gcggcgtgaa aatgcagagt gacctggaga
    12181 gcggcgaccg gatcaccatc catagtcatc agttcagcgt cccgcgtctg gaaagtcatc
    12241 tgtttgatgc caacaacacg cgcattctta accgcgtggt attccgtaac gaaaccctgc
    12301 aacagattat ccaggcgatg tcgttaagcc gcccggccaa agggcgcttt aaccgccgcg
    12361 gacgtatttc ttatcgccag ttgggtatca accagttggg tgcggtgtat gaggcgctgc
    12421 tctcctatca cggattcttc gccagcgaag atctctacga ggtgaagaaa gccggggaag
    12481 agtttaacga gctggagacg ggttacttcg tcagtaagga tgagattggc aaataccacg
    12541 aagacgagaa ggtctacgag aaagacggca gtctgcgcat tcaccgcaaa ggtagcttta
    12601 tctaccgtat ggccgggcgc gaccgtgaga aatctgcctc ttattacacc ccggaagtgc
    12661 tgacccgctc actggttaaa tatgccctga aagaactgtt taaggagcag attgatccca
    12721 ttagcgatcc gcacgccaaa gctgatgcca tcttaaacct caccgtgtgc gaaccggcga
    12781 tgggcagcgc ggcgttcctt aacgaagcca tcaaccagct ggcggaagcg tatctgttcc
    12841 acaagcagca ggctgaaggt cgccgtattc cgcaggatcg ttacacccag gagttacagc
    12901 gggtgaaaat gtacattgcc gacaacaacg ttttcggcgt ggacttaaac ccggtggcgg
    12961 tggaactggc ggaagtgtcg ctgtggttga acgccatcag tggcgatgcc tttgtaccgt
    13021 ggtttggtta ccagctgcac tgcggtaact cactggtggg cgcgcgccgt caggtgttca
    13081 acaagagtga actgacctac aaaaaagcca aagatccgag ctggcttaac agcgagccgg
    13141 tcgagctggc gatgaacacg ccgcgtgaag agacgcagat tttccacttc ctgctgcccg
    13201 acggcggtat ggctaactac agcgataaaa ccgttaagca gcgttatccg gatgacttca
    13261 aagcgctgga cagctggcgc aaagagttta ttaaaagctt tgccgggcat gagattgctg
    13321 atgtgcagcg tatcagcgaa aaggtggaag cactgtggaa cacctatcgc cagcaactta
    13381 aagcagaacg tctgaaaacc gccgacagct acccggtgtg gccggcagaa aacagcgagc
    13441 agacgcgttc ttcgctgagc agtaaagatg aaacctttag cggtcgtctt gaagataaca
    13501 gcgcctacca gaagctgcgt tgggtgatgg actactggtg cgcgctgtgg ttctggccga
    13561 tcgacaaagc cgatgagtta ccggatcgcg gcacctggtt gtttgagatt gaaaccctgc
    13621 tcgacgggat tgtaatcacg gaaaaagtca ctgaagttgc ggagcacacc actggcgatc
    13681 tgtttgccga agacggcctg ctgcgagaag agtcttcgct gttttccgtt gctggtcgtc
    13741 tgaaaaccga ggtgttgttc cgtcatttgc cgcgtctggc gattgtcgat gccctgagaa
    13801 agcagcaccg tttcttccac tgggatctgg agttctgcga cttgtttgcc gagcgtggcg
    13861 gttttgacct gatgctcgga aacccgccgt ggctaaaagt ggaatggcag gaagcgggcg
    13921 tgctgggtga ttacgagccg gaatttgtgc tgcgtaagct gagtgcatcg aagctggcaa
    13981 cgttgcgtat tgataccttt aaccagatcc cggcgctgga agcggcctgg cgcagcgagt
    14041 atgaaggctg tgaagggatg caaaacttcc tgaatgcgca gcagaactac ccggtactag
    14101 caggacaaaa agcgaatttg tataaatgtt ttctacctct ggggtggcgc ttaggggcgc
    14161 agaaaggtgt cgtagggttc ctgcatccgg aaggaattta tgaagacccg aaagggggaa
    14221 aactgcgtgg ggcggtctat ccaaggttaa gagctcattt gcagtttcaa aatcagcata
    14281 tgcttttccc tatcggtgac agggttaagt ttagtattaa tatctacacc gcgtttgtaa
    14341 gtgatcatat tggcttcatt agtattgcaa acttattctc ggcaaaaacg attgaactgt
    14401 gtttgtcgca tgatggaact ggagaagtgg gtggtattaa agatgacgaa accgactcat
    14461 ggaacgaatc aggacaccaa agccgcatat tgaaagtatc gttagagcgg ctttccttgt
    14521 ttgctaattt gtatgatgag ctaggtatac ctgcgattga ggccagatta cctgctcttc
    14581 atgcggatca cttggtgtct gtgcttgaga aatttgctaa gcagccaact agattgagtg
    14641 gcttagaaaa agactattat tcaacccaac attggaacga agttaatgct caggatgatg
    14701 ggataatcag acgcgaaacc caattcccgg aagacgcatc ccaatggatt ttgtctggtc
    14761 cgcacttcta tgttggaaca ccgctttata aaacgccgag agaaatttgc acagagaaag
    14821 gacattatga ctgcctggac ttgctaactc tgcctgacga ctatctgccg cgcactaact
    14881 atattccagc atgtgatgca caggagtatg caaagcgcac cccacgggtt tcgtggaaag
    14941 aacaggatga agacgagccg agaaaggtga cggattatta tcgttttgtt aatcgtgaaa
    15001 tgataggtcc atcatctgaa agaacattaa ttacaacaat tgtgccacca ggtgtgagcc
    15061 atataaatac ttgtcttgga acagcgttta aagatactca attattgctt gactattttt
    15121 cgatgactct ttctgtaccg ttggattatc gagtgaaaag tacgggtatg gggcatgcca
    15181 atacctctct tattaaccaa ctgccagtac ttacaaatga tgcttatcgt acagcactac
    15241 atagtcgggc tctatgcctt gtatcgctag tgagaagtta cgctgatctc tggcaatcct
    15301 gctacacccc agacttcaac acccagcgct ggagccgcaa tctcccgcag ctcccccagg
    15361 atttcttcgc caaactgacc ccagagtggc agcgtaactg cgccttacgc tctgactaca
    15421 gtcgtcgtca ggcgctggtg gaaatcgacg tactggtggc gcaggcgctg gggttaaccc
    15481 tcgaagaact gcttaccatt taccgtgtac agttcccggt gatgcgccag tacgaagcgg
    15541 atacctggta cgatcaaaac ggtcgcatta tctttacccc aagcaaaggg ctggtgggcg
    15601 ttggcttgcc tcgcaccgcg cgtaaagctg acctgaaaaa cggctttgtc tttaacgtcg
    15661 acagcccaga gtggaccggc ggtgactgca ccgatcaagc catcggttgg gatgatgtca
    15721 aacatctgca aaccggtacc gtcagcgtca cctttgatga ctatacccgc agcgacgaag
    15781 gcgagcgccg taccgtcacc tggcaggctc cgtttatcaa gcctgatcgc gaagacgact
    15841 acaaagtggc ctgggcgttc tttgcacaag ataaggagag cgcctgatgc tgccgtccgt
    15901 tgtcagtcgg caggtcgccg acagcgttgc cgccttttta cgggcggcgt tcccgttaaa
    15961 cagcccgctg ttcaacggtg agaataacga taatgtctca atgctggagc aatttctgtc
    16021 tcagccggaa acgctgctga aagggccgta tctttccgct cagctgccgt ttcgtaaaag
    16081 tgacttaccg ctgaattttt tccctaatct gacgctgccg ttcccgccgc acgcacatca
    16141 ggcccgggca tttcagcgtc tgggaagcga cgcgccacag ccgacactgg tcgctaccgg
    16201 taccggctcc ggtaaaacag aatgctttat gttcccgctg ctcaaccact gcgcgggcgc
    16261 gtcacaagcc ggggttaaag caattatcat ctacccgatg aacgccctgg caaccgatca
    16321 ggccagccgt ttcgccaaaa ccatcgccag cgatccacaa ctgcatggca aggtgaccgt
    16381 cggcctgttt gtcggcgaca gcgaaatcga acccagcaaa aaaatgtcgg cagaaaaggt
    16441 cattacctgt aagcacacgc tgcgcgaaag cccgccggac atcctgttga ccaactacaa
    16501 aatgctcgac tacctgctga tgcgccctgg cgatcagccg ctctggcgtt acaaccagcc
    16561 cggttcattg cgctacctgg tcgtggatga attgcatacg tttgatggcg cgcaagggtc
    16621 ggatctggcc tgtctggtgc gacgtctgaa gcatcacata ggtgtagata accaacgatt
    16681 cgcctgtgtc gggacatcag ctaccgtcgg cgacgagctg gggcaactgc tcgattacgc
    16741 aaaaacaatc tttgaccagc cgtttaccga tgacgccgtt attcgcgaag atcgctacac
    16801 ggcagcggag tacttgcagg attacgccat tgcgtacagt cagtatccgg ggccggaggt
    16861 cgcttcggcc ctggacccgc agggttacgc taccccggtg gcgtatctca acgggcaaat
    16921 ccctttgtgg ttccccgaca gcgacttgca actcccggac gatctggaga gcgacgacgg
    16981 gcgtgaaaag cgtattgcgc tggggtcact gttacgccgc cacagcgtga tgcatgtctt
    17041 actccatgat ctgcaaggcg ggatcctctc tgaacgagag tgtctggaaa atctccaggt
    17101 catgctggca gaaagcgccg accatgcccg acgcgtgctg caaagcattc tggcgctgat
    17161 cgcgcaggca cgtctcgaag taccggaaac ggaacacgat cggcaaaaac gtctccaggc
    17221 gaagaaagcg cgtccggtgc tgccgtttgt gcaactgcgt tcccagttgt ggcttcgtga
    17281 gatgcgtcgt ctggtcgcca gcgtggcgaa aacgccgaga ctggtgtttg ccgatgatgt
    17341 ggcggtggaa gaccgcgaac actatttgcc ggttatccac tgccgtgact gccacgcaac
    17401 cggctgggcc agcctgcgtc atgggcaaag cgttcagctg gaaacagacc tcgacggcat
    17461 ttatcgccag ttctttgaaa aagggcgctc catcgtcctt gctttcccgg ataacaacga
    17521 aaaagcggtc agtggcgcac accagaaact gtgccctatc tgtctgaaac tgaaccgaca
    17581 gtccagcgtc cagtgtggac attgcggtca tacggaactg ttgcaggtac tgatcccgga
    17641 tatgctcaaa gagcgtaagg acgagctgga gtttgctaat gaatgcccgt actgcaacag
    17701 caaacagggg atttcgattc tggggtcacg ggctgccagt ttgtcggcgg tgatgattca
    17761 ccagctttat ggcagccagt tcaacgaaca taaaaagctg attacgtttt ctgactcggt
    17821 acaagacgcc gcgcatcgtg ccagcttctt tactgcccgt acctggccgc tgatgattcg
    17881 tgggcaaatc gccagtgttc ttggcgaaaa agagacatta ccgctggatg cgttcgcggc
    17941 gcgtgtttgc caacaaacgc gtgaacgttc accggacgat gctcattttg cagcgaactt
    18001 catcgcacca aatatgcagt ggctgaatga ttatcagaca ctgaaaaatg aaggcaaact
    18061 ggcggagaac tccgatctgc cggagctggt ttccctgcgc ctcgactggg aggtgtacag
    18121 cgaatttacc ctgcgcgcgc gtatcggacg tacgctggaa gccaccggtt atgccgttgc
    18181 cggagtggat atagaacgct ttaatccggc aattaccgcc ctgcatcaac agctttgtga
    18241 agagctgggt gatgagatgt ccggtgtgag cgtcgagcag gtagcgcacc tggcgctggg
    18301 ggttatctgg tatcaacgcc aggccggggc cgttatgcat ccggcgttcg aaagttatcg
    18361 ccgtgatggc acgaccttta tgatgacgca aaaagagcgt acatcgcgct atatgccttc
    18421 tatcggtccg aaaacgcgta aaccggctta tgcgtcgttt gaaaaaatta atggtttcca
    18481 tcgcctgatt ggcgcgaagt ctaaccccag ttggtatcag cattggatca atcgcacgct
    18541 gagtaacggc aataatctgt ttatcagtag cgttgcggaa acagtgttac gacgcctgtt
    18601 tacggcgttg aaaatagccg gagtagtaaa agattttgac accaaagggc gtgaagcctg
    18661 gggactggtg ccttcggcgc tggtggtgag ccgtgacgtc gttcagctta attgctcatg
    18721 ttgccgtgag gttgtccggg ctccggccga tcaaggctgg cagtggcgta atgcgccttg
    18781 tctctcttta cgttgtgaag gtcgttacca ggaagtcgac aatatggcga cctggcgttg
    18841 ggagaacatg gacatcgcgc gcgtgcaggg cgcagaacac acggggctgc ttagccgtga
    18901 agatcgcgaa gccaccgaaa aatcgttcta ccgcggcaac caaccgtgga atatcaacct
    18961 gttgtcggca acaccgacgc tggagatggg tattgacgtt ggggatcttt ccaccgtttt
    19021 actctgttct gtcccacctg ctcaggccaa ctacttgcag cgtattggtc gcgccggtcg
    19081 taaagatggt aacgcgttaa acattaccgt ggcagaaggg aacccgcacg atcagttttt
    19141 ctttgaagaa ccactggaga tgatgcaggg ccaggtgcag gctcctggcg tcttcctcaa
    19201 tgcaacggct attctcgaac gccagctggc ggctttttgt atggataact gggtgaaaac
    19261 cggtgtacca gaatccgcta tctgtaaaaa cgtcaaacag atgctcgatg agctggagtt
    19321 tgggtgtaaa tcgggttttc cgtataactt actgagatat atcgaacagc atcatgcgga
    19381 gattgctgca caatttacgg caatcttccc cgatcttgcc gcagaaactc gccaacagtt
    19441 gttgtcttat cttcagggcg cgccaggaca acgctctctg gtgcagcgta tcgaagaggc
    19501 gttaaaactg ctggtagaag atcgcaaatc tctgcgttca cgcatcgata aactcaagcg
    19561 cagtatcgat aaactggaga atgcgccgcg cgatcaaaac tttgacagcg atatgcgcga
    19621 gttaacttcg gaacggcagg cgctgatggc gttggtcaat caaatcaaca ataagcaaac
    19681 gctgaatttc ctcacggatg aaggtctgct gcctaactac gccttcccgg aagccggtat
    19741 tacgttacgt tctgtgctct ggcggcgtaa agaaggcggg gaagcacgtg aatatcaaaa
    19801 caccactttc gaatatgaac gcccggcatc aacggcgctg gctgagctgg ctccgctgaa
    19861 taatttctat gccggtgggc ataaagttga aattgagcag atagacctga aggtctctga
    19921 gccggagaac tggcgtatct gtagccactg taactacagc gaaaatatcg accagacggg
    19981 cgaccagcat aaatattgcc cgaaatgtgg cacaccgggg tgggccgacg ccggacaaaa
    20041 aaccacgctg ctgaaacttc gccaggttta tgcccgcgcc agcgcacgtg atagccagat
    20101 cagcgacgag agcgacagtc gtgaacctgc tttcttccag cgtcagctat tggttagttt
    20161 cgaaaaagag gatgtatcgg cggcatacgc gattgaggaa ggcgaagttc cgtttggttt
    20221 tgaattttta agcaaagtca ccctgcggga tatcaacttc ggcaaaatgg cggatgatgc
    20281 caatgagttg atgattgccg gagaagcgaa gaagcgaacc gggtttaaag tttgcctggg
    20341 gtgcggcatg gtacagcgtc ctcgcgatca tgaaccacgt catgatttga gctgcaaata
    20401 tcgcacggaa ccggaaaagg cgaagttcga agattatctc tatctctatc gccagctgga
    20461 gtcagaagcg ttgcgaattc tgctcccggt aagcagttac agtaatgacc gtgtcgtgga
    20521 ggcttctctt ggggcggcga tccagctcgg ccttaaacat tacttcaagg ggaatgtcga
    20581 tcacctgaaa ggcgttgtgt accgcgagcc ggaaaatgaa ggcgaatcct ggcggcagta
    20641 tctggtgatt tacgataccg tcccgggggg aactggttct cttaaagagc tgatgcgcac
    20701 tcccgacaat ttgctgaaat tacttgaact ggcctatcag gcgatagtgg aatgcagttg
    20761 caaccatgat acgcataaag atggttgtta tcgttgcgtt tacgcttacc gtgaccgtgg
    20821 gcgcatgaag tatgtctccc gcgatcaggc gcgtctcttg ctggcgaaaa ttttgcaggc
    20881 aagagattcg atacgggtaa ttgattccat caaaaacatt tccctcgaag ccatgatggg
    20941 cagtgagctg gaaaaacggt ttattagctg cctgcaagaa aataaaaacc tgattgtcag
    21001 tcgcagttac gcgcatcagg gcgctgggtg gattattaac acccgcagcg aaccgatgat
    21061 gagctggcat cttaaagctc aggtggattt aggcgctaaa gaggcggtgg ggatcccttg
    21121 tcgcccggat tacgtgctat atccgcttat gcagtctgat gccattaaac cggtagctat
    21181 tttccttgat ggctttgcgt ttcacaaaaa tagcgtcgca gaggatgtgc aaaaacgcca
    21241 ggcgattcgg gatagcgata atttctgggt gtggacagtc acgtgggccg atctcaaaga
    21301 gcctgggctt aaacatgttc aggatgtcct ggggcttggg cataacccgg atatgaaaca
    21361 gcctaagttt tataacctgt ttcacgatac caactttgcg gcccttgaag cgtcgttccg
    21421 ggaacgcaac agttttgcac tgctgctgga ttatctcgcc gatccgggag gaagaacgca
    21481 gttatggcag aaaatggctg ctgcacatgc ctgggtgtgg ttggatgtga agaagtcaca
    21541 ggacgcggga acgaaacaaa agtatgccta tgaaatgcag gaaaacgcgc cagactggcg
    21601 gctagcagag cttttgccag acgaaccgtt tgtctttggt ggtttactcg atagctgtaa
    21661 ctcatcgcag caatttattg agctggcttc ggtgttgcca caacaagcca ttaaaccgac
    21721 gaccagtgtc gcacaaatgc gcagctggct gcgtttgcat atctgttttg acgatcgcta
    21781 tacgcaggat gacggttatg aagcgggact caatggcttc tggcgactgg taaacctgtt
    21841 acagttcctg ccggatataa cctttaccag ccgtaaagcc gtgcatttac cgcaggaaag
    21901 cgttgacact aaagtttcgg caacgccaga ggcagtacca gagactgatg agagttgggc
    21961 tgagattatt gagtttggct tattaagcgc tgaagagatc gcgttgcttc agtctcattc
    22021 tctctctgcg ccgactctcg gctacgaatt gcaaaatgat gagggtgaaa ttattgcgga
    22081 agccgatctc gcctggccat cacaaaaatg ggcattgatt attgataatc aagaatttat
    22141 tccattattc acaaccaggg gttggcacgt tgcattcggg ccaattgacg aaaatacatt
    22201 gcaacatctg tccggaggtg acaaatgacg gtgcaaacgc ccaaggtggc actttctgat
    22261 ggttttctgg gcgcattcgc gcggatccca aaagcacagc aaaaaaaggt gcaggaattc
    22321 atctccaaat tccgtcagga cccgaccagt aatggactta actatgaaaa gatccacgat
    22381 gcgcgcagta aaaatgtcca ttcggtgcga atcgatcaaa cttatcgcgg cattgttctg
    22441 aaacctgaac agggtgccct gtatatgctg atgtgggtgg ataaacacga cgaagcatac
    22501 gactgggcgc ggcggcatga ttgttcgatt cacccggtta ccggcgcaat tcaggttatt
    22561 gatattagct atatcaaacc ggcaccggaa acggtggttg ataaaccgaa actcttcgcg
    22621 gcttacagcg cggaacagat tctggccctg ggtgtgccgc ctgtctttat tgatcaggtg
    22681 atggcgctta ccgatgaagc cgggcttaat caacttgaaa gcataatgcc agcagaagcc
    22741 tgggaacctc tgcactggct ggcagaaggg ctggattacc aggaagtgct ggaagagttt
    22801 aacgatcatc gtgatgagcc tgttgatacc aacgacttta tggaggcgat tgaacgaagt
    22861 aaacgtcgct tccatgtcgt tgaaaatgaa caggagctgt tgcagatgct taatgcgcct
    22921 ttagaaaaat ggcgtgtatt tctgcatccg agccagcgaa aactggtgga gtcgcccgct
    22981 aatggtccgg tacgagtact gggcggtgca ggtacaggga aaacggttgt tgcgatgcac
    23041 cgtgcgcggt ggttatctca gcgtctggca gataaacccg gtaagaaggt acttttcacg
    23101 acctttacgc gtaacctggc ggcagatatt cgcgctaatc tccagcggtt gtgtacgcga
    23161 gaagagatgg cgcgtattga cgtcgttaac attgatgcct ggatgagcga ccaactaaaa
    23221 cgccacggtt atgatttccg cgtggtatac gacagcgatg aagggcgacg taaatgctgg
    23281 aactatgcct tgcagcaggc acctgccgaa ctggggctac ctgacaattt ctatgctgaa
    23341 gagtggcaac gcgttgtcca gccaaatgcg gtttacaccc gggaggaata cctcaaagtc
    23401 agccgggttg gacgtggtac agcgttatcc cgtattcaac gggcgaaaat ctggccagtt
    23461 ttcgaagaat tcagagcaca aatggcgcgt gccaaactgc gcgaaatggg cgatgccatg
    23521 catgaggcta ttgttctgtt taaagaaaag caggtccaac taccttatag cagtattgtc
    23581 gtcgatgaag ctcaagatat cggtgccccg gcatttactc ttattcgcag cctggttccc
    23641 gagtcgccca acgatctgtt tattgtcggg gatgggcatc agcgtattta tcgcaataag
    23701 gtcgttcttg gtcagtgtgg tattaacgtc cgtggtcgtc gcagtaaaaa attgaagatt
    23761 aactatcgga ccacagaaga gacgcgtcaa tttgctgttg ggctgttaac tggtgtgaag
    23821 gttgataacc tcgatggcga agcggatacc agtaatgatt atttgtcatt actgcacggg
    23881 gaaaagccca tgattacgca tgcggcagat ttcaaagaag aggctgcaac gctcgtcaag
    23941 caaattcagg cattgctggc gaatcaggtt cgttctcagg atatctgcat tactgcgcgt
    24001 actaaacatg cttgcgaccg ctacgcatca gcactcaatg acgccggaat tgagacattt
    24061 aatttaggca atgatagcgg tgacagcgat gcacgcccag gcgtgcgagt tgcaacgatg
    24121 caccgtatta aagggcttga gttccagtat gtgttcctgg tggggattaa cgaaggcgtt
    24181 gtgccggaaa taaaagcatt agcatccgat gatcccgttg aacagcgtga tgcattattt
    24241 aatgagcgcg cgttactgca tgtggctgca acccgtgccg taaaagggct gtttgtttca
    24301 tcatctggta tccctagtcc gctattagtg gctgattgat aatatgtccg aaacagacca
    24361 ggagccgaaa cgctcctggt ctatataacc gttctggcaa ctggcgatat ttatattgtt
    24421 tagctcccaa cagccagaca atatcagctt agaaattata ttcgcttatg cggttagcag
    24481 gtgatgctgc caacttacta atttagtgta tgatggtgtt tttgaggtgc tcctgtggct
    24541 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
    24601 agcactgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
    24661 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
    24721 ggcgttggat gccgggcaac cgcccgcatt atgggcgttg gcctcaacac gattttccgc
    24781 catttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccg
//
LOCUS       DAPPNJ010000056        24442 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000056 DAPPNJ010000000
VERSION     DAPPNJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.056"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(173..249)
                     /locus_tag="RPM73_001840"
     tRNA            complement(173..249)
                     /locus_tag="RPM73_001840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Arg,seq:acg)
     gene            complement(253..345)
                     /locus_tag="RPM73_001841"
     tRNA            complement(253..345)
                     /locus_tag="RPM73_001841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Ser,seq:gct)
     gene            complement(661..846)
                     /gene="csrA"
                     /locus_tag="RPM73_001842"
     CDS             complement(661..846)
                     /gene="csrA"
                     /locus_tag="RPM73_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDX6520209.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1081..3711)
                     /gene="alaS"
                     /locus_tag="RPM73_001843"
     CDS             complement(1081..3711)
                     /gene="alaS"
                     /locus_tag="RPM73_001843"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDX6520210.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3839..4339)
                     /gene="recX"
                     /locus_tag="RPM73_001844"
     CDS             complement(3839..4339)
                     /gene="recX"
                     /locus_tag="RPM73_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDX6520211.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4582..5643)
                     /gene="recA"
                     /locus_tag="RPM73_001845"
     CDS             complement(4582..5643)
                     /gene="recA"
                     /locus_tag="RPM73_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDX6520212.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5723..6220)
                     /gene="pncC"
                     /locus_tag="RPM73_001846"
     CDS             complement(5723..6220)
                     /gene="pncC"
                     /locus_tag="RPM73_001846"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDX6520213.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6365..7450)
                     /gene="mltB"
                     /locus_tag="RPM73_001847"
     CDS             complement(6365..7450)
                     /gene="mltB"
                     /locus_tag="RPM73_001847"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDX6520214.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7706..8269
                     /gene="srlA"
                     /locus_tag="RPM73_001848"
                     /gene_synonym="gutA"
     CDS             7706..8269
                     /gene="srlA"
                     /locus_tag="RPM73_001848"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDX6520215.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8266..9225
                     /gene="srlE"
                     /locus_tag="RPM73_001849"
                     /gene_synonym="gutE"
     CDS             8266..9225
                     /gene="srlE"
                     /locus_tag="RPM73_001849"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDX6520216.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9236..9607
                     /gene="srlB"
                     /locus_tag="RPM73_001850"
                     /gene_synonym="gutB"
     CDS             9236..9607
                     /gene="srlB"
                     /locus_tag="RPM73_001850"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDX6520217.1"
                     /translation="MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9611..10390
                     /gene="srlD"
                     /locus_tag="RPM73_001851"
     CDS             9611..10390
                     /gene="srlD"
                     /locus_tag="RPM73_001851"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDX6520218.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10495..10854
                     /gene="gutM"
                     /locus_tag="RPM73_001852"
     CDS             10495..10854
                     /gene="gutM"
                     /locus_tag="RPM73_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDX6520219.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10921..11694
                     /gene="srlR"
                     /locus_tag="RPM73_001853"
                     /gene_synonym="gutR"
     CDS             10921..11694
                     /gene="srlR"
                     /locus_tag="RPM73_001853"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDX6520220.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11687..12652
                     /gene="gutQ"
                     /locus_tag="RPM73_001854"
     CDS             11687..12652
                     /gene="gutQ"
                     /locus_tag="RPM73_001854"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDX6520221.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12649..14163)
                     /gene="norR"
                     /locus_tag="RPM73_001855"
     CDS             complement(12649..14163)
                     /gene="norR"
                     /locus_tag="RPM73_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDX6520222.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVILSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14350..15789
                     /gene="norV"
                     /locus_tag="RPM73_001856"
     CDS             14350..15789
                     /gene="norV"
                     /locus_tag="RPM73_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDX6520223.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15786..16919
                     /gene="norW"
                     /locus_tag="RPM73_001857"
     CDS             15786..16919
                     /gene="norW"
                     /locus_tag="RPM73_001857"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDX6520224.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17145..19397)
                     /gene="hypF"
                     /locus_tag="RPM73_001858"
     CDS             complement(17145..19397)
                     /gene="hypF"
                     /locus_tag="RPM73_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDX6520225.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19550..20077)
                     /gene="hydN"
                     /locus_tag="RPM73_001859"
     CDS             complement(19550..20077)
                     /gene="hydN"
                     /locus_tag="RPM73_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDX6520226.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20226..21236)
                     /gene="ascG"
                     /locus_tag="RPM73_001860"
     CDS             complement(20226..21236)
                     /gene="ascG"
                     /locus_tag="RPM73_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDX6520227.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21496..22953
                     /gene="ascF"
                     /locus_tag="RPM73_001861"
     CDS             21496..22953
                     /gene="ascF"
                     /locus_tag="RPM73_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDX6520228.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPVQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            22962..24386
                     /gene="ascB"
                     /locus_tag="RPM73_001862"
     CDS             22962..24386
                     /gene="ascB"
                     /locus_tag="RPM73_001862"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDX6520229.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
BASE COUNT         5717 a         6714 c         6281 g         5730 t
ORIGIN      
        1 ttttttgata ccgctactaa agccgtatca agtaagagat ggtgcatccg ggaggattac
       61 tcgccttgcg gctcgccctt cgggccgttg ctaaagcaac gttatcctcc ctggtgcctg
      121 cggttatacc gccacctctt ccataccttc atcgctgaaa gtacgtagaa tatggtgcat
      181 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
      241 tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt ttgaccgcat
      301 actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg cctcttacga
      361 gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata ttactttctg
      421 agacttataa gtcaaacaat ttttcctaca cttttatcgt ttgcacactt cacgttcaat
      481 tagtctgtaa aaacgtcaaa aagagtgttt tatcaacaga aaaatggagg tctgacagat
      541 agtagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag cgaaaagact
      601 atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga cgcggaaaga
      661 ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct cttcacggtg
      721 aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct ttacccctaa
      781 aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac gagtcagaat
      841 cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc ccggcattat
      901 ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag cagtcatggc
      961 attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg actttatcgt
     1021 tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct gacgcttata
     1081 ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag gtaaggccgc
     1141 agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc cgcccacctg
     1201 ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt ccttagatac
     1261 gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa ttgtcgaccc
     1321 cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa caccgctaag
     1381 ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa gatttgcgct
     1441 ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact gacgcgtacg
     1501 ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca gcagatgcgc
     1561 gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt ctccggttac
     1621 cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc ggaacagacc
     1681 aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga aatcgcccat
     1741 gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg cacctttcgc
     1801 tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac gaatctgtgc
     1861 gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt tgtgtgagaa
     1921 gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat gagtccccag
     1981 aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac gaatacgggc
     2041 gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag aacccgcagc
     2101 aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca ccgcgaagga
     2161 gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt ccgcatagaa
     2221 tggtgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat caaccgcttt
     2281 accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat agcctttaaa
     2341 ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc cgctggcttc
     2401 gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt caactttgat
     2461 gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg tgtcgtacag
     2521 acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct cttcatccag
     2581 caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct tcagcacctg
     2641 ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa cgtcgatcag
     2701 cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc cgtgacgcac
     2761 tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg gcattacgcc
     2821 atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca gcgatttatt
     2881 gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc ggaacaggtc
     2941 gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca gacccatacc
     3001 ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc ggttgaactg
     3061 catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg gacttcccgg
     3121 agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg ggccgcacgg
     3181 accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt tgttatcgcc
     3241 gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt cgtaggcttc
     3301 gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa accatttttc
     3361 gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt cgccgaagct
     3421 gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga cgttttccag
     3481 gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc gggaataatt
     3541 acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt tggtaaacaa
     3601 caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat gtcccttact
     3661 atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca taattatcct
     3721 gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc cgggctggta
     3781 actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc gtatgcgttc
     3841 agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca cgatagagca
     3901 gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca ccatattttc
     3961 gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt
     4021 ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt gcaggtccat
     4081 aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcgagatagc
     4141 catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca tcaatctctt
     4201 ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt
     4261 gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac
     4321 gggacgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat
     4381 gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag aatgttcccg
     4441 caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt gctggcctgt
     4501 tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca cacattatct
     4561 attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc cttcgctgtc
     4621 atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac gtactttctt
     4681 ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat tcgctttacc
     4741 ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga tcagcttctc
     4801 ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt agaggatctg
     4861 gaattcagcc tgtttaaacg gcgcagcgat tttgttcttc accactttca cgcgggtttc
     4921 gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt cgagacgaac
     4981 agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac cgaacatcac
     5041 accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact gcttcaggtt
     5101 acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca tgtgagagtc
     5161 gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt caacgacgat
     5221 aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct gctcgccggt
     5281 gtccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg cgtagattgg
     5341 gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct gcgctgcggc
     5401 gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga tttcgacgat
     5461 acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg aaccggtaga
     5521 gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc ctttaccaaa
     5581 ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt cgtcgatagc
     5641 catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg ttgaagcaat
     5701 tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac aaggtctgca
     5761 atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag cattcccgcc
     5821 gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg acaggcttct
     5881 cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa tcagcacgag
     5941 ccgctttcag tgcgcctatc gccatttcca ccacgacggg ttcactcacc gcgccatgct
     6001 gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta ctgtaggtga
     6061 caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact ttcgctaccc
     6121 aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc agcgcctgcc
     6181 caacctgttc acttaactgc atcagttcac tgtcagtcat cacaacctct gtcagtcaaa
     6241 aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt tgcaatttta
     6301 cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc cctcttgtaa
     6361 atagctactg tactcgcgcc agcgccacgg cttgtcctaa ctgccagacc gccattgcgt
     6421 aatgggtgct gtggttgtaa cgggtgatgg tgtagaagtt cggcagaccg taccagtact
     6481 ggtagccggt gccaacatcc agacgcagca ggctggcttg ttgatggttg cccagcggct
     6541 gctgtggcgt taaacctgcg gcggcaagct gcgaaatgct gtacttagtt ttgaagccat
     6601 ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac ctgatcgcct ttcacccagc
     6661 cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc cacaggttga
     6721 tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc ataaactgtc
     6781 cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc tgctcgtcgc
     6841 gcgccatcaa caggaaggtt tccagctcgc cagagaaata ctccgcgcgg cgtgggtagt
     6901 taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg cgcccccagc
     6961 gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac acctgccacg
     7021 cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc acgttgtccg
     7081 gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt cggacctgat ggcggtttca
     7141 ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga cgtttcgcct
     7201 gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc attttgtcga
     7261 tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc acattgtgct
     7321 gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtatcagtc tcagtaggtt
     7381 ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt acataacgac
     7441 gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg taaagcatca
     7501 cgccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa ctcaactcat
     7561 tcccctcgct atccttttat tcaaactttc aaattaaaat atttatcttt cattttgcga
     7621 tcaaaataac acttttaaat ctttcaatct gattagatta ggttgccgtt tggtaataaa
     7681 acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg cagagtggtt
     7741 tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg gcattcttcc
     7801 gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg gtcagcatcg
     7861 tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc tactgttacc
     7921 gtgcattggc acgtttgtct tttgcaatcc gatgacccta agcctgggtc gctttatgcc
     7981 ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact caatgaatgg
     8041 cctcttcccc catatcaacc ctggcgaact gtttgtttat cttggcattg ccagcggtct
     8101 gacaacgctg aacctgccac ttggcccact ggcggtgagt tatctgctgg ttggtctggt
     8161 caccaatttc ttccgcggct gggtgaccga tctgaccacc gccatttttg agaaaaagat
     8221 gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac gcatattcgg
     8281 atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tgaaagccac gccgggaaaa
     8341 aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact ggcacagctt
     8401 actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt
     8461 gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa acgacgtatt
     8521 cccaccatta atatccactc gacgggcaag tccggtccgc tggcgcagta cattgtggaa
     8581 gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtaggtga tgcgacacca
     8641 caaccctctt ccgtgggccg tgactatgac accagtaaga aaatcaccga acaaagcgat
     8701 ggtttactgg cgaaggtggg aatgggcatg gggtccgccg ttgcggtgct gtttcaatct
     8761 ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc attcgtctcg
     8821 gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca cggtcttgct
     8881 ccgctggcga gccatccact gggtctggtc atgctggcgc tcatctgttc cttcccactg
     8941 ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt attgattggc
     9001 gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact gtttgccatc
     9061 aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga agcccgtcag
     9121 gacacggttc gcgtcggtgt cccttctgta ctggtgagcc gctttttaac cggcgcgccg
     9181 actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct gaaacatgac
     9241 cgttatttat cagaccacca tcacccgtat cggcgcgagt gccattgacg ccctcagtga
     9301 ccagatgctc atcacctttc gtgaaggcgc gcctgcggac ctcgaagagt attgcttcat
     9361 tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaatttt cactcgggca
     9421 gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa gacaaccttc gcgaactggg
     9481 tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca ctgtccatgt
     9541 ggcaggccct gtccccgacg atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa
     9601 ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacat taggcgcgtt
     9661 cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga
     9721 caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaagta tggcgtacgg
     9781 ttttggtgct gacgccacta gcgagcaaag cgttctggcg ctctctcgtg gggtagatga
     9841 aatctttggt cgcgtggatt tgctggtcta tagcgccgga atagcaaaag cagcctttat
     9901 cagcgacttc cagctcggcg attttgaccg ttcgctacaa gtgaatctgg tgggttattt
     9961 cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac gggattcagg gtcgcattat
    10021 tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc
    10081 gaaatttggt ggcgtcgggc tgactcaatc actggcgctg gatctggcgg agtacggcat
    10141 tacggtgcat tcactgatgc tcggtaacct gctgaaatcg ccgatgttcc agtcactgtt
    10201 gccacaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacatcga
    10261 caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg ctgaatatgc tgctgttcta
    10321 cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt
    10381 gatgttctga tcaacagcgg agatccgtta aggatctccg tgagactata gaatgcctga
    10441 tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta
    10501 tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg
    10561 caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg ggtcggcgtg
    10621 ggccgttcca gcgggcgctt taaaccgcgg gtcgtggtcg ccatcgccct ggacgatcag
    10681 cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa
    10741 attcccgcaa ttaccggtat gcatgcgggt gatttacagc ccgatgtgat ctttccccat
    10801 gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt
    10861 tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc
    10921 atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc
    10981 tcggttgaag aattggcgca atactttgac accacaggca caaccattcg caaagatctg
    11041 gtcattctgg aacatgccgg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa
    11101 gaggaatccg atccgcctat cgatcataaa acgctcatca acacccacaa gaaagagctg
    11161 attgcagaag ctgccgttag ttttattcat gatggcgact caataattct tgatgctggc
    11221 agtaccgttt tgcagatggt ccccctgctc tcgcgcttta ataacatcac ggtgatgacc
    11281 aacagtctgc atatcgtcaa tgcgctatct gaactggata acgaacaaac catcctgatg
    11341 ccaggcggaa catttcgcaa aaaatcggcc tcatttcacg ggcaactggc agagaatgcc
    11401 ttcgagcatt tcaccttcga caaattattc atgggtactg acggcatcga tctcaatgcg
    11461 ggcgtaacca ccttcaacga ggtctatacc gtcagtaagg caatgtgcaa tgccgcgcgc
    11521 gaagtgattt taatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc
    11581 agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccggc gtttcgtcag
    11641 gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact
    11701 actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc gtttaccgga
    11761 acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt
    11821 ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg caacacttgc
    11881 cagtaccggc acaccggctt tttttgtcca tccggcagaa gcactgcacg gcgatctggg
    11941 gatgattgaa agccgcgatg tgatgctgtt tatctcttac tccggtggcg cgaaggaact
    12001 ggatctgatt attccgcgtc tggaagataa atctatcgcg ctgctggcga tgaccggcaa
    12061 accgacgtca ccgctgggcc tggcggcaaa agcggtgctg gatatctccg tagaacgcga
    12121 agcctgcccg atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga
    12181 cgcgctggcg atggcggtca tgcaggcgcg cggttttaat gaagaagatt ttgcccgctc
    12241 ccacccagct ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg
    12301 tgatgacgct atcccgcagg tagcgttagc cgccagcgtg atggatgcga tgctggagct
    12361 cagccgcacc ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt
    12421 ctttaccgac ggcgatttac gtcgctggct ggttggcggc ggcgcactca ccacgccagt
    12481 taatgaagcg atgacgacag gcggcaccac attacaggcg caaagccgtg ccatcgacgc
    12541 caaagagatc ctgatgaagc gcaaaatcac tgccgcaccg gtggtagatg aaaacggcaa
    12601 actcaccggc gcaattaact tgcaggattt ctatcaggcc gggattattt aatccttcaa
    12661 tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtca gtttccagca ttcgcgcgca
    12721 ggcagcccag ttgtgatgat tttgtgccag tgcctggcga atggtttcac gctggaacgc
    12781 ttctgtcgct tcacgcagat tttgcttaac aacgggcacc gctgccgctt ctggcggcgg
    12841 caacgtcacc tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgctgcg
    12901 agtggctctc gacagtacta ccgcccgatg aatagcatgt tccagttcgc gcacgttccc
    12961 cggaaaactg tagtgttgca gtaaatttcg cgccccggca cttaatatca cgcgggagag
    13021 ccccagccgc aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc
    13081 ccgctcacgc agcggcggca ccgaaagtgg aaacacgctc aggcgatgaa acaagtcagc
    13141 gcgaaatcgc cctgccagta cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg
    13201 cacatcgacc cgcaaactgc ggtcatcgcc aacgcgctga atatcgccat actgcaacac
    13261 tcgcagcaac ttggcctgca aagccaacga caactcgccg atctcatcga gaaacagtgt
    13321 gccgttatcc gccatttcaa acttcccgct gcggttactg atagcgccag taaacgcccc
    13381 tttcacatgc ccgaacagtt cactttccgc cacactttcc ggcagtgcgg cacagttgag
    13441 atagatcagc ggattcaccg cccgtggcga ggcttcatga atcgctttcg ccaccagctc
    13501 cttaccggtt cccgtctcac cgctgattaa gacgttgaga tcggacgccg ccacaatctc
    13561 aatctctttt ttcaattgcg tcatgcctgg cgataagcca atcatctgcg tctgtttcac
    13621 cgcttcaaac ggcgcggcat cgcccggcag catattctgg ctttccagtt gctcaatcag
    13681 caacgcattg cttaacgctc ccgccgccag cgcagcaatc agccgtagct cttcgtcgct
    13741 gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc agtgcgccga tcaggttttg
    13801 cccggcaaac aatggcagac caacgcaggc gtgaaccttc agactctcct gcccaggaat
    13861 caaaccgtca tagggatcgg gcaattcgct gtctgcagga aagcgcacca cgtccccggc
    13921 gcgggcaatc gcttccagcc gtggatgccc ttccagcgca aagcgtctac cgagtacatc
    13981 ctttgccaga ccgtcgatgg caagcggaat aaactgccgc gaatcgtaac gtagcaacgc
    14041 agacgcatcg cactccagca cctgacgtag cgtggtgatc aggcgctgaa aacgatcctg
    14101 atgaccaatc ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact
    14161 catctttgcc tcactgtcaa tttgactata gatattgtca tatcgaccat ttgattgata
    14221 gtcattttga ctactcatta atgagcatac ttttatttat atagtaaaaa caatcagata
    14281 aaaaactggc acgcaatctg caattagcaa gacatctttt tagaacacgc tgaataaatt
    14341 gaggttgcta tgtctattgt ggtgaaaaat aacattcatt gggttggtca acgtgactgg
    14401 gaagtgcgtg attttcacgg cacggaatat aaaacgctgc gcggcagcag ttacaacagc
    14461 tacctcatcc gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa attcagccgc
    14521 gaatttgtgc agaacctgcg taatgaaatc gatctggcgg atatcgatta catcgtgatt
    14581 aaccatgcag aagaggacca cgctggggcg ctgaccgaac tgatggcgca aattcccgat
    14641 acgccgatct actgtactgc caacgctatc gactcgataa atggtcatca ccatcatccg
    14701 gagtggaatt ttaatgtggt gaaaactggc gacacgctgg atatcggcaa cggcaaacag
    14761 ctcatttttg tcgaaacacc aatgctgcac tggccggaca gcatgatgac ttacctgaca
    14821 ggcgacgcgg tgctgttcag taacgatgct ttcggtcagc actactgcga cgagcatctg
    14881 ttcaacgatg aagtggatca gacggagctt ttcgagcagt gccagcgtta ctacgccaat
    14941 atcctgacgc cgttcagccg cctggtaacg ccgaaaatta ccgagatcct gggctttaac
    15001 ttaccagtcg atatgatagc gacctcccac ggcgtggtct ggcgcgataa cccgacgcaa
    15061 attgtcgagc tgtacctgaa gtgggccgct gattatcagg aagacagaat caccattttc
    15121 tacgacacca tgtcgaataa cacccgcatg atggctgacg ctatcgccca gggaattgcg
    15181 gaaaccgacc cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa
    15241 atcctgacca atgtcttccg ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac
    15301 gtgatgatgc cgaaaatcgc cgggctggtg gaggagatga ctggtttacg cttccgtaac
    15361 aaacgcgcca gtgctttcgg ctctcacggc tggagcggcg gtgcggtgga tcgtctttcc
    15421 acgcgcctgc aggatgcggg tttcgaaatg tcgcttagcc tgaaagccaa atggcgacca
    15481 gaccaggacg ctctggagtt atgtcgtgaa cacggtcgcg aaatcgcccg tcagtgggcg
    15541 ctcgcgccgc tgccgcagag cacggtgaat acggtggtta aagaagaaac ctctgccacc
    15601 acgacggctg acctcggccc acggatgcag tgcagcgtct gccagtggat ttacgatccg
    15661 gcaaaaggcg agccaatgca ggacgttgcg ccaggaacgc cgtggagtga agtcccggat
    15721 aacttcctct gcccggaatg ctccctcggc aaagacgtct ttgaagaact ggcatcggag
    15781 gcaaaatgag taacggcatt gtgatcatcg gttcgggctt cgccgcccgc caactggtga
    15841 aaaatattcg caaacaggac gccactattc cattaaccct gattgccgcc gacagcatgg
    15901 atgagtacaa caaacctgac ctcagccatg ttatcagtca ggggcaacgt gccgatgacc
    15961 ttacccgcca gacggcgggt gaatttgccg agcagtttaa tctgcacctg tttccacaaa
    16021 cctgggtgac ggatatcgat gccgaagccc gtgtggtgaa aagccagaat aatcagtggc
    16081 aatacgacaa gctagtactg gcaaccggtg ccagtgcctt tgtcccgcct gtgcctgggc
    16141 gtgagttaat gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc
    16201 gggatgcccg acgcgtgttg attgttggcg gtggtttgat tggtagcgaa ctggcgatgg
    16261 atttttgtcg tgcaggcaaa gcggtcacgc taatcgacaa cgctgccagt attctggcgt
    16321 cattaatgcc accggaagta agcagccgct tgcagcatcg gttgacggag atgggcgttc
    16381 atctgttgtt gaaatctcag ttgcaggggc tggaaaaaac agattctggc attctggcaa
    16441 cgctggaccg ccagcgcagt atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc
    16501 cggaaaccgc cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata
    16561 gttatctgca aaccagtaat gccgatattt acgcgctggg cgattgcgcg gaaattaacg
    16621 gtcaggtatt gccgttcctc cagccgattc aacttagcgc gatggtgctg gcaaaaaatc
    16681 ttctcggcaa taacacgcca ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg
    16741 aattaccgct gcatctggca ggcgaaaccc agcgtcagga tttacgctgg caaattaata
    16801 ccgaacgcca gggaatggtg gcgcgcggcg ttgacgatgc tgaccagctt cgcgcctttg
    16861 tggtcagtga ggatcggatg aaagaggcat ttggattgtt gaaaacattg ccgatgtagg
    16921 tgggctactg tgcctaaaat gtcggatgcg acgctggcgc gtcttatcca acctacgggg
    16981 agcgcatgtg ttggtcacat actgcgttca tgtcgcatcc acctactgtg cccaaaatgt
    17041 cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag gccggataag
    17101 gcgtttacgc cgcatccggc aatggtgtcc aaatgcaaca cgttttatcc gttctggact
    17161 tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa accgccatca
    17221 cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg cgccagacgt
    17281 gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt gataccacgc
    17341 atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc
    17401 cacgcgcgtt gattaaccgg tgcctgccag ttcagccact gctgccagaa agtggcgaga
    17461 tccagttgat tgtccaccag cggcattgtc accggatgcg tcactccgtg gcacgaagct
    17521 gcgagtgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc tggcgcacag
    17581 cccagtgccg ccgccacggc atcgaacaac cgcccacacg atgacgccag cggcgcgtta
    17641 attccacgct caatggcccg cgccagcacg ctccagtttt gctgttgcac acttgctgtt
    17701 tcagaataat tctgccactc cggcacaaag cgcaggcact gcgccagcag gtttcgccac
    17761 ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc gcccaggtgc
    17821 tcacattcgc gatagttcac ccgcagacac tcgccgcccc acaaagcgcc gttctccccc
    17881 ataccgatac catcgagcgt caaagcaatg acatcaccgc catccagcgg ccactgatgc
    17941 tctgccagac acgccgctgc gtgggcatga tgatgcagca ccgtttgcgt cggcatattc
    18001 atttcgctcg cccactggct ggagacatag cccggatgca cgtcatgcac aacgtattgc
    18061 ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg catctggatg
    18121 ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc gcgcaccagg
    18181 cagaaggtgt ttttcagatc cgcgccaaga cacagcacag gcggaacatt tttaaagccc
    18241 ggaggcaaaa ccagcgcatc cggcacatac ccgcgtgaac ggcgcagcat ttcgccgctt
    18301 tcgcgcacca ccgaatcatc catccgctgc acgatgtcgc ggttatgtat caagaatccg
    18361 tcggcaatgc cctgcaaatc ctccagcgcc tgttcgttgc tgatagccgg tggtttaccg
    18421 ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag caaatgctgg
    18481 agcgggttcg caggcaacat taccccgact tcgttaaggt caggggcgat atcatcacaa
    18541 agctcaggaa cgtatttttt atccaccagc acaatcggcg cggcgggcgt ggtaagcaac
    18601 tggcgcgcag cgtctggtaa accgtcagcc actggcaaca tgaccgccag cggtttcgcc
    18661 gggcgatgtt tgcgcgcccg aagtgtcgcc accgcgttac tgttacgtgc atcgcaggca
    18721 agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata
    18781 gccgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca ttcaagatgt
    18841 gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg
    18901 tactctttgt cacaagccgg acacaacgga aacgccgcca tcacggtaaa cgggcggtcg
    18961 taaggcatgg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt gataaacgga
    19021 taacgataac gccgttcgcc tggggtattc atttcggcaa gacaggcagg gcaagtagcg
    19081 gcatcgggaa caatttgcgt attcatggtg ccgcctgtgc tctggcgtat agtgaactcg
    19141 gtgggcagtt gtgaccagat gtacggctca cgctcgacgc tatcaatacg cgccagcggc
    19201 gggcagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag ccggacttct
    19261 acgccatcgc cgtcattaca gacatcgccg tgaagattta attgctgtgc cagctgccag
    19321 acaaacggac gaaaaccgac gccctgcact ttgcctcgaa tacgcagttg gacaccgcaa
    19381 gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc cgccctgccg
    19441 gacacgactg cgtcgtatcc ggcagtctca ggtcggcgat accgccagct ccgtattcta
    19501 cgaatatttc cgggaattcc tttgatgcca gaacagttct ataagatttt tagaacatca
    19561 gcgccgtacg gcgacgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa
    19621 tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcgcga tggttgcaca
    19681 ggtcgcattt attggcttca gctttgtcag cccgtacatt cagccccgcg ccgctgttgc
    19741 ggatcaccgg acgtaccacc acttccatcg cgccatacgg gcaagccacc acgcaggttt
    19801 tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg ctgatagcac
    19861 cattcgggca gacgttagcg cacggtgcat cttcacactg acggcagact gtcgccgtgg
    19921 aaatgttcac acctttaatg acatggattc gcggtaaaaa ggtttccggg gtcagcgatg
    19981 cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa
    20041 tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc aatgacagtt
    20101 aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt taaggaaatt
    20161 aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac atgtgatgac
    20221 aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt
    20281 ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat aatttcctga
    20341 atcatctctg ttaccggaat ttttacgctg gagagcgccg gaacggtgta gggggcaatt
    20401 gcgatatcat cgaatccgat aaccgacacc tgctctggca ccgctacgcc gcgctcgtgc
    20461 aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta
    20521 gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt ccatttaccg
    20581 ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc tttatacccg
    20641 gcaagacgtt caacgctggt gggggaatcc atcgagccgg taaggaaagc aatctcctga
    20701 tgtccggcgt ttatcaactc tgccacggca ttaaagctgg tctgtttatg atcgcaccag
    20761 acgctatggc tgctgttttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgc
    20821 gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg
    20881 tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta
    20941 ccatctgcca atagcaactg ccgccctttc tcttccgcca ttcgcgcggc atgaaacagt
    21001 aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc cagcgtctga
    21061 gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaaccgct ctcttctacc
    21121 gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg
    21181 gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt
    21241 ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc
    21301 aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg aacaattaac
    21361 tctcttgctt atttagtgac aactattcat gattttgtga aaccggtttc ctaattccgt
    21421 ttcagcatcg gcatttttcc gttacgtcga ctgataacaa ctacatctac cctactgata
    21481 acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca
    21541 ctgggcggcg ttgataacat ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt
    21601 atcaaagatg accagcttat cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc
    21661 gtggtacgta gtgacaacca gtgtcaggtg attatcggca ataccgtttc acaagccttt
    21721 caggaagtcg tcagcctgct gccgggagat atgcagcccg tacagcccgt gggtaaaccc
    21781 aaactcacgc tgcgtcgcat tggtgcgggg atcctcgatg cgctgatcag caccatgtca
    21841 ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag
    21901 atgagcggcg tgttgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac
    21961 ggtgctttct tcttcctgcc gctaatggtc gcggcatctg ccgccatcaa atttaaaacc
    22021 aatatgtcgc tggcgattgc catcgcaggt gtgctggtac atccgagctt tattgaactg
    22081 atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgattccggt caccgcggtg
    22141 aaatatacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa
    22201 cgctgggtgg atcgcattac accggctgtg accaaaaact tcctcaagcc aatgctgatt
    22261 gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat ctggatcggt
    22321 agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc
    22381 attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt ctttacgcca
    22441 accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc
    22501 ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg
    22561 gaactgcgcc agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa
    22621 ccggcgttat acggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc
    22681 ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg
    22741 ccggggttat ttaccagcgt gcaattcttc gatccggcga atccaatgag catcgtctgg
    22801 gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta tcctcacact attgctcggc
    22861 tttgaggata ttcctgttga ggaagcggct gccgaggcgc gaaagcatca gagcgcacaa
    22921 ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag
    22981 gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgttc cgtgaaggtg
    23041 gcaaaggact gaccacggtc gatatgatcc cgcacggcga gcatcgaatg gcggtgaaac
    23101 tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga
    23161 cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaagg
    23221 ttttccgtac ctcaattgcc tggagccgcc tctttccgca gggcgatgaa atcacgccca
    23281 atcagcaggg cattgctttt taccgttctg tctttgaaga gtgtaaaaag tacggtatcg
    23341 aaccgctggt cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct
    23401 cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgccagaacc tgctttgaag
    23461 catttgatgg tctggtgaaa tactggctaa ccttcaatga aatcaacatt atgttgcata
    23521 gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga aaatcaggat caggtgaaat
    23581 atcaggccgc gcatcaccag ctggttgcca gtgcgctagc caccaaaatc gcccatgagg
    23641 ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg taacttctac ccttacagct
    23701 gcaaaccgga agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg
    23761 atgtgcaggc gcgcggcgcg tatccggctt actctgcccg cgtatttcgc gaaaaagggg
    23821 taaccatcaa caaagcaccg ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct
    23881 ctttcagcta ttacgcctcg cgctgcgcct cggcggagat gaacgccaac aacagcagtg
    23941 cggcgaacgt ggtgaaatcg ctgcgtaatc cgtatctaca ggtgagcgac tggggctggg
    24001 gaattgatcc actcggtctg cgtatcacca tgaatatgat gtacgatcgt tatcagaagc
    24061 cgctgtttct ggtggaaaac ggcctcggcg caaaagatga acttgctgcc aatggcgaga
    24121 ttaacgatga ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga
    24181 ttgcagatgg cattccgctg atgggctaca ccacctgggg ctgtattgat ttagtttccg
    24241 cctctacggg tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt gacgacgcag
    24301 gcaacggcac gctgacgcgc acgcgtaaga aatcgttctg gtggtataaa aaagtgattg
    24361 ccagtaatgg tgaagattta gagtaagtaa ccgtgccgga tgcggcgtga acgccttatc
    24421 cggcctacat agttcgtgcg ac
//
LOCUS       DAPPNJ010000057        24386 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000057 DAPPNJ010000000
VERSION     DAPPNJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.057"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            146..1351
                     /gene="gltS"
                     /locus_tag="RPM73_001863"
     CDS             146..1351
                     /gene="gltS"
                     /locus_tag="RPM73_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDX6520230.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1354..2220
                     /locus_tag="RPM73_001864"
     CDS             1354..2220
                     /locus_tag="RPM73_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520231.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISERDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2205..4286)
                     /gene="recG"
                     /locus_tag="RPM73_001865"
     CDS             complement(2205..4286)
                     /gene="recG"
                     /locus_tag="RPM73_001865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDX6520232.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4292..4981)
                     /gene="trmH"
                     /locus_tag="RPM73_001866"
     CDS             complement(4292..4981)
                     /gene="trmH"
                     /locus_tag="RPM73_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDX6520233.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4988..7096)
                     /gene="spoT"
                     /locus_tag="RPM73_001867"
     CDS             complement(4988..7096)
                     /gene="spoT"
                     /locus_tag="RPM73_001867"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDX6520234.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7115..7390)
                     /gene="rpoZ"
                     /locus_tag="RPM73_001868"
     CDS             complement(7115..7390)
                     /gene="rpoZ"
                     /locus_tag="RPM73_001868"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDX6520235.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7445..8068)
                     /gene="gmk"
                     /locus_tag="RPM73_001869"
     CDS             complement(7445..8068)
                     /gene="gmk"
                     /locus_tag="RPM73_001869"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDX6520236.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8326..10008
                     /gene="ligB"
                     /locus_tag="RPM73_001870"
     CDS             8326..10008
                     /gene="ligB"
                     /locus_tag="RPM73_001870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDX6520237.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWEEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10005..10622)
                     /locus_tag="RPM73_001871"
     CDS             complement(10005..10622)
                     /locus_tag="RPM73_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDX6520238.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10914..11738)
                     /gene="dinD"
                     /locus_tag="RPM73_001872"
     CDS             complement(10914..11738)
                     /gene="dinD"
                     /locus_tag="RPM73_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDX6520239.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11959..12822)
                     /locus_tag="RPM73_001873"
     CDS             complement(11959..12822)
                     /locus_tag="RPM73_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDX6520240.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12949..13665
                     /gene="rph"
                     /locus_tag="RPM73_001874"
     CDS             12949..13665
                     /gene="rph"
                     /locus_tag="RPM73_001874"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDX6520241.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13731..14372
                     /gene="pyrE"
                     /locus_tag="RPM73_001875"
     CDS             13731..14372
                     /gene="pyrE"
                     /locus_tag="RPM73_001875"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDX6520242.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14409..15005)
                     /gene="slmA"
                     /locus_tag="RPM73_001876"
     CDS             complement(14409..15005)
                     /gene="slmA"
                     /locus_tag="RPM73_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDX6520243.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15112..15570)
                     /gene="dut"
                     /locus_tag="RPM73_001877"
     CDS             complement(15112..15570)
                     /gene="dut"
                     /locus_tag="RPM73_001877"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDX6520244.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15548..16768)
                     /gene="coaBC"
                     /locus_tag="RPM73_001878"
     CDS             complement(15548..16768)
                     /gene="coaBC"
                     /locus_tag="RPM73_001878"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDX6520245.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16940..17608
                     /gene="radC"
                     /locus_tag="RPM73_001879"
     CDS             16940..17608
                     /gene="radC"
                     /locus_tag="RPM73_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDX6520246.1"
                     /translation="MKNNSQLMMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17825..18061
                     /gene="rpmB"
                     /locus_tag="RPM73_001880"
     CDS             17825..18061
                     /gene="rpmB"
                     /locus_tag="RPM73_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDX6520247.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18082..18249
                     /gene="rpmG"
                     /locus_tag="RPM73_001881"
     CDS             18082..18249
                     /gene="rpmG"
                     /locus_tag="RPM73_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDX6520248.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18347..19156
                     /gene="mutM"
                     /locus_tag="RPM73_001882"
     CDS             18347..19156
                     /gene="mutM"
                     /locus_tag="RPM73_001882"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDX6520249.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19195..19674)
                     /gene="coaD"
                     /locus_tag="RPM73_001883"
     CDS             complement(19195..19674)
                     /gene="coaD"
                     /locus_tag="RPM73_001883"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDX6520250.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19682..20959)
                     /gene="waaA"
                     /locus_tag="RPM73_001884"
     CDS             complement(19682..20959)
                     /gene="waaA"
                     /locus_tag="RPM73_001884"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDX6520251.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21372..22430
                     /gene="rfaQ"
                     /locus_tag="RPM73_001885"
     CDS             21372..22430
                     /gene="rfaQ"
                     /locus_tag="RPM73_001885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDX6520252.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22427..23551
                     /locus_tag="RPM73_001886"
     CDS             22427..23551
                     /locus_tag="RPM73_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDX6520253.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23544..24341
                     /gene="rfaP"
                     /locus_tag="RPM73_001887"
     CDS             23544..24341
                     /gene="rfaP"
                     /locus_tag="RPM73_001887"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDX6520254.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5711 a         6027 c         6580 g         6068 t
ORIGIN      
        1 ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa agcataactc
       61 gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga gtatgaaaga
      121 gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa cgcttgttgc
      181 cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct ttttgaagaa
      241 atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc tactagtact
      301 gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc cgttaatgct
      361 ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg ccggtgggcg
      421 tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa atgccattgg
      481 cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg ccggttctat
      541 tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca ttgaacgtta
      601 tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc tggtgctggg
      661 cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca cgccgaacgg
      721 tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg gacgcatgat
      781 cacctcgctg gtgctgattg aaactatcgc gctgattgct atctgcctga cggtggggaa
      841 aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg tctgtgtact
      901 gtttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc gcgtctttga
      961 gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat
     1021 ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga ttctggtggt
     1081 acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga tgggcaaaaa
     1141 ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac
     1201 ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca tggcgttttt
     1261 ggtggtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg tgattaagct
     1321 gtatttaatg ttgccgattt ttgccggtta aaaatgaaaa agcgtcggaa ggaaccggaa
     1381 acgctacgtg aacactgtcg ccatattttt ggtgatgagc cgccagtgct ctgtgtatgg
     1441 gaaacggaat ttgattacgc agatgccgaa ctcaaggcgc tggcggctaa agagtggcag
     1501 cagatcagcg aacgggattt gagtgcttac tatgtactca atctcgttta taacgaaccg
     1561 atgcaaatag agttgtttcg ctatctgttt ccgctctgcc tggcgcaatg gcatgaaacc
     1621 gttttagctg gcggttatgg tgatcacttc gaagaatcct taatgaaggc attgtgtcga
     1681 ccctatctct ggcaggagat gatgaatgct tcccaacgcc agcaggtacg gcaattttta
     1741 ctggacaccg cgttgcagcg tatggataac gagcgtggtt tcaataacgt tctttgctgg
     1801 ctggcggtgt tcaatactct gggtggggcc gcgccgttaa ttcgttctct ctggtcgcgc
     1861 tggtgggcgc tggatacgcc aggaaaagcg gtgtgtgcta ttcagtatgc cgcgcatcta
     1921 atttatccca tagaagccaa tccgttatgg tcgcaagaat ggataggctg ggggcatcca
     1981 ttaggccata aagatggctg gtcctctgat aatcgcgctt ttcttcgcca aatgttaaca
     2041 cctgaaatga ttgtggctgg cgtacaggca gcggctgaaa ttctgcgtgg tgaaccggaa
     2101 ggcgcaatgg cggcgcgaat tgcacaggat gcgtatgagg caatggatat cctcactatc
     2161 cagattgaag atttactgcg tgacttgtct tgtgatgagt ctggtcatgc gttggagtaa
     2221 cgttccgtct ccggcatcca gcgttctatc agggcttttg cttgttgtgg gtaacgttcg
     2281 tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc
     2341 gccactttaa actcagcatt acccgtctga cgcgtaccta acagttcacc cggtccgcga
     2401 atctctaagt ctttctgtgc aatcacaaaa ccgtcgttgc tgtcgcgcag cacttgcagc
     2461 cgaatttgcg cggttttgga gagcggcgtt ttgtagagca gcacgcagtg agaagccacc
     2521 gcaccacgac ctacacgccc gcgaagctgg tgtaactgcg ccagacccag acgctccggg
     2581 ttttcgataa tcatcagact ggcgttaggc acatcaacgc cgacttcaat aacggttgtg
     2641 gcaaccagca ggtgtagctc accttgttta aacgacgcca tcaccgcctg tttctcggca
     2701 ggtttcatcc gcccgtgtac caggccaacg ttcaactctg gtagcgccag tttcaactct
     2761 tcccaggtag cttccgccgc ctgcgcttcc agcaattccg actcttcaat caacgtacaa
     2821 acccagtacg cctgacgacc ttcagttatg caggcgtggc gcacgcggtc aatgatgtcg
     2881 gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg cggcagctca
     2941 tctatcaccg aggtatcgag atcggcatac gccgtcattg ccagcgtgcg ggggatcggc
     3001 gtggcggtca tgatcaactg atgcggatgg aagccttgct gctggccttt ctcccataac
     3061 gccagacgct gatgcacgcc aaagcgatgt tgttcgtcga taatcaccag cgccagaccg
     3121 ttaaactgca cctgttcctg gaagatggcg tgcgtaccaa caatcatctg cacctgaccg
     3181 ctggcgatgg cttcctgctg agacagtcgc gctttgcctt tctgcttacc ggcgagccag
     3241 cccacttcga taccgagcgg ttcaaaccag ttgcggaagt tattggcgtg ctgttcggcg
     3301 agtaattcgg ttggtgccat caatgccacc tgtttgccgt gggcaatcgc acgcagcgcg
     3361 gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgtaccag gcgcatcatc
     3421 ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg tgcgcccgtt
     3481 ggcttgaacg gtaaggctgc gaggagttta tttttcagcg catcattggc gctcagcggc
     3541 tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt gtgcgccagc
     3601 agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag atcgctaagc
     3661 tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct
     3721 tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag atccagcgcc
     3781 tggtcggtta atttacgcag cgtggcctgc tttacgcctt ccgttgttgg ataaaccggc
     3841 gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta ttccgggtgg
     3901 atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac acggcggccc
     3961 gtcgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt gaggatgccg
     4021 gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat attgcagttc
     4081 agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat ggggtagaga
     4141 tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac ggtatgcagg
     4201 ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga actgagtggg
     4261 acagcatcta acaggcgacc tttcatggca cttaccctgc agcctgcata gtagcccacc
     4321 agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc aggcctttgc
     4381 gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt tgttgctctg
     4441 cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc tgacgctggg
     4501 cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc atgccgatca
     4561 tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg cccgttttct
     4621 cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt
     4681 tatcagaaag atgggttgcc agaatctgca tgccccggcc tttgagatga gcgacggcat
     4741 cgccaatggt gcggtgtgtt ttcacctgta cccagctgtt actacccgcc gctgccgaag
     4801 ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg ccaacggcat
     4861 ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc atgcagacgg
     4921 tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt gttgggttca
     4981 taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg cggattttgc
     5041 gcatgatatt cgccagatgc acacggtcac gggcggtcag acgaataaag gcgctgtaga
     5101 cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaagtcgtg gtgttaattg
     5161 ccgccgtcag gtttgccagc gcgccctgat gattgaacat ctccaccttg atttcggtga
     5221 tgaactcctg cgccgtctct ttatcccatt ccacagccat aaacttctct ggctctttct
     5281 ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta ccggggctga
     5341 cgtgggcgat aatcgggtcg ccaggaatag ggcggcagca tttcgcaaag gtgatcagca
     5401 cgccatcggc acctttaatg ggcagatgtc cgtggctttg ggttgccggt ggaatggagg
     5461 cgtccccatg ttgcagattt ttcgcgacca ccacgctcat tgcgttacca agtccgattt
     5521 ctgccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc tgaatatttt
     5581 cctgcgggat ttcattgagc ttacggctac cacccaaagc atggttgagc agacgacggc
     5641 ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt ttggcgcgcg
     5701 cttttgagct aacgacaaag ttcagccacg cggcattcgg gcgagcgccc ggtgcggtaa
     5761 tgatttcaac ggtttgaccg ctggtaagcg gctgcgacag cgggtaaggc tggcggtcaa
     5821 cgcgtgcgcc cacgcaggca tgaccgatat cggtatgcac tgcataagcg aagtcgacgg
     5881 gcgttgcacc ggcaggcagc tcgacaattc gcccttccgg tgtgaaaacg taaatctcat
     5941 ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg gcgctctgtt
     6001 gcagctccag caggctttgc atccagcgct gggcgcggat ttgtgcggta gtactggttt
     6061 cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc tgatccatat
     6121 cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc gaggtgtgca
     6181 acgactgata gccgttcgct ttaggaatgg cgatatagtc tttcacgcga cccggacgcg
     6241 gcttgtacag gctatgcatt tgcccgagca cgcgataaca ggtgtcagaa tcattgacga
     6301 tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg agcaccattt
     6361 tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt attcccgctt
     6421 cctgcaaacg cccttcgatt tcagagagga ttttttggat catctcttta cggttgccgc
     6481 gcgcggcttt caccacttct ttaattacgc ggtaacgatt gggatacagc gcctcaaaac
     6541 ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc agcgggctat
     6601 aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg
     6661 tgcgcatgtt gtgggtacgg tcggcaagtt tgatgagaat gacgcggata tcctgcacca
     6721 tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg cggaacttga
     6781 gcttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca aaaagctgtt
     6841 ccatgtcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc gccgccatca
     6901 gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc gggtgcgtga
     6961 tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg
     7021 cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc
     7081 tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca gcaatagcgg
     7141 taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga
     7201 ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca gtggttttat
     7261 cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga gcgcgacgcg
     7321 cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc tgaacagtta
     7381 cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga tactgaaatc
     7441 aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt tggcggctca
     7501 tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta tcgaagtcat
     7561 cattcacaat cagataatca tattcggcgt aatggctcat ttctgcaaca gcttgcgcca
     7621 tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt
     7681 caattttgga cggcggtaaa ataaagatac tccgcgcgtg cggcattttc tggcgaattt
     7741 gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc agtacttgct
     7801 caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg
     7861 catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa tgttcaccgt
     7921 ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca
     7981 acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca ctgggggcag
     8041 aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta gcgaaataaa
     8101 gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt gctcacactt
     8161 tttgcgcaaa gttgttgcgt tttgcgtagc tgtttccgca tttgcctgtg atttactgca
     8221 ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc ggctttgcct
     8281 tcaccacgtt gtatatctgc caacctgact acagcggagg tgaagatgaa agtatggatg
     8341 gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg tccggcctgg
     8401 tcgccagcca gagcacagga agaaatttcc cgcctgcaac agcaaataaa acagtgggac
     8461 gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga tcagttaagc
     8521 gcccgtctta cgcagtggca acgctgcttt gggagcgagc cccgcgacgt gatgatgccg
     8581 cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa aatggtggat
     8641 aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca gccaaaagtt
     8701 gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc aatcagtcgc
     8761 ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc cgctgtgccg
     8821 caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt tctccagcgc
     8881 gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt tgctggcctg
     8941 atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt ctgggcatgg
     9001 ccggatggac cgcagttaat gaccgatcgt ttaaaggagc tggctaccgc aggttttact
     9061 ctaacgcaga ggtatacccg tgcggttaaa aatgctgatg aggttgcgcg cgtacgcaat
     9121 gagtggtgga aagcgaaatt acccttcgtc accgatggcg tggttgtgcg aggggcgaaa
     9181 gaacccgaat cccgccattg gctaccaggc caggcagaat ggctggtggc ctggaaatat
     9241 caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg taagagcggt
     9301 aaaatatcgg tggttgcgtc actcgcacct gtcatgctgg atgataaaaa agtccagcgg
     9361 gtgaatattg gttccgtcag gcgctgggag gagtgggata ttgcgcctgg tgatcagatc
     9421 ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtctg gcgcggtgca
     9481 gaacgtacaa aaccgacacc accagaaaac cgctttaact cgttgacctg ctactttgct
     9541 tctgatgttt gtcaggaaca gttcatttca cgcttagtct ggctggggtc aaaacaggtt
     9601 ctggggctgg atggcattgg tgaggccggc tggcgcgcgc tgcatcagac tcatcgcttt
     9661 gagcatatct tttcctggct tttattaacg ccagagcaat tacagaacac gccggggatc
     9721 gcgaaaagta aaagtgcgca gctatggcat cagtttaatc tggctcgtaa gcagcctttt
     9781 actcgctggg tgatggcaat gggaataccg ctaacccggg cggcgcttaa tgccagtgat
     9841 gaacggtcct ggtcgcaact tttatttagc acggagcagt tctggcagca gctgccgggg
     9901 actggatcgg ggcgcgccag acaggttatt gaatggaagg aaaatgcgca aatcaagaag
     9961 ctgggcagtt ggctggctgc ccagcagatc acaggttttg aaccttagtg tccttcgtgg
    10021 ctgtagtaaa agaccggtaa tcccagctta aggcgtagcg ccagtaagcg ggcaaaaaag
    10081 ccgaatacca gggtggagat aattaccaca tcatggttag aaacatagtg ttgcagcgcg
    10141 atgtacagca cggcggaggc aaatgagaca ccggcataca actctttctg gaataccagt
    10201 gggatacgtt tacagaacat atcgcgcaaa acgccgccaa acacgccggt ggttaccgcc
    10261 gcgacaacgg caataattgg gccgtgcccc atatccagtg caacctgtgc gccgatgata
    10321 gaaaagacca ccagtccaag cgcatcgagc accagaaaca ctttgcgcag gtaaggcatt
    10381 acaggagcaa cgatagtggt gagtactgcg gcggtagcga cgataatcac atattccggg
    10441 tgtttgaccc agccgagcgg atagtggccc agcagaatat cgcgcactga ccctccgcca
    10501 attgcggtgg cggtcgcaat aataattacg ccaaatgtat ccatgcggcg tcgcccggcc
    10561 gccagagccc ccgtcatggc ttcggcagta atgccaacca aatacaaaat gtgtaacagc
    10621 atggttctct ccctaaaaat ctgtctggca gggtagcgat ttgtgcgtat tgtcacgatt
    10681 gagatttttt aaggtgagag gaaattgata acttgtagtt atgtgaaatc gctattttct
    10741 gtagcgtaga tgcattcttc tgacttcttg tctgagaaaa tgcataagaa aaaataggtt
    10801 aaaattgagg ggtggaaaag aaaggtgcaa tgtttagcca ttgtgttcac cgcacctgga
    10861 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
    10921 ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac
    10981 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct acccacgtca
    11041 aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
    11101 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
    11161 ttctgactct ttttcagccc cttcagttgg tggatggctt tctgatctaa tccaccatac
    11221 agcccctggt aaccatgatt ctgaaatatg gcgaaatcag tagctgtcgc taccccagct
    11281 tgctgtgctg cctcaaccaa ttgtttgtta tgttctttca actcatttct cagaaacaga
    11341 cgcttttcgt cttcacgaag ttgcttaaat gcttcatcgt cagcaagctc ctgccgtcgg
    11401 gtctggatag caaaataagt ctgccctgcc gcaatgaccg gtttcgcagg gtcgccgttt
    11461 tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc tcgttgagcc
    11521 cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc agcttgattg
    11581 ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga
    11641 taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc agtgccataa
    11701 tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
    11761 ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc tcacgttctc
    11821 agtgtgctgg gcaaaaaaac atcgcaaaat ctggagcagg cacgcaaaac agaattgaag
    11881 ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag gctgaaaagt
    11941 aagaattagt gttacgagtt actcaatatt ctgaatctgc tcgcgcatct gctcaatcaa
    12001 cactttcagc tcgatggcgg agtttgtcac ttcggcattg atagatttcg acgcaagagt
    12061 gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa cggcttcttt
    12121 tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca gttcttcggc
    12181 aacgtcaatt cgttgtgcca gcagaaccag ctcctgctcc agacggttgt tttccaactg
    12241 aacctgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca ggatttccgg
    12301 catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct gctcgatcaa
    12361 tgctttcagc gcctgacctt cggtttcgcg cgcgacgata aagtcgtcca gcgtaccatc
    12421 caacgccgcg agaatttcag cggcaatcgc gtcgagatcc tgctcctggg ctgccatcac
    12481 gcccggccag cgtagaatat caaccgggtt gatttcccct tcgtcactct gcatttttac
    12541 ccagttcgcg gcagttacca gctgtttagc cagtttttca ttgaggatca gctcaccttg
    12601 tgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg tcaggcgaga
    12661 acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca gacgaaagta
    12721 agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc cccattcacc
    12781 cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc ctgtttataa
    12841 aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat aaccgccgga
    12901 agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat gcgtccagca
    12961 ggccgtagca ataatcaggt gcgtcccgtt accctgactc gtaactatac aaaacatgca
    13021 gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc ctctattgaa
    13081 gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc agagtacggt
    13141 atgctgccgc gttctaccca cacccgtaac gctcgtgaag cggcgaaagg taagcagggt
    13201 ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg
    13261 aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc tgatggtggc
    13321 actcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcgct acagaagctg
    13381 gtggaaaacg gcaagctgaa aaccaacccg atgaaaggga tggtagccgc agtttctgtc
    13441 ggaattgtga acggcgaagc ggtttgcgat ctggaatacg ttgaagactc tgccgcagag
    13501 accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca ggggacggca
    13561 gaaggcgaac cgttcaccca cgaagaatta ctcaccttgt tggctctggc ccgaggggga
    13621 atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt aaggcgactg
    13681 atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat
    13741 atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta
    13801 cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc
    13861 gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt
    13921 tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact accgccgtgg
    13981 cgctggcgga gcatcatgac cttgacctgc cgtactgctt taaccgcaaa gaggcaaaag
    14041 accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta atgctggtag
    14101 atgatgtgat caccgccgga acggcgattc gcgaatcgat ggagattatt caggctaatg
    14161 gcgcgacgct tgctggcgtg ttgatttcgc tcgatcgtca ggaacggggg cgcagcgaga
    14221 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc
    14281 tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg
    14341 ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa aagtcatccg
    14401 gcgtcatatt actgcaactg cgcggcaatt agcggccagc gggcgtcaaa atcatccgtc
    14461 gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca gaaggccagg
    14521 atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg cattctcttt
    14581 tcacgcaata cctggcgcag ctgcgcttca atacgctcga acagctggtt gatgcgccct
    14641 tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt caggccagga
    14701 ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct
    14761 ttctcatctt tcagaatcag gttgatgcga gtaatcaggc tatcttcgat aaactcaatc
    14821 aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc ttcggaaacg
    14881 ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct ggattccagc
    14941 atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt ttgtttttct
    15001 gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca cgctatgacc
    15061 gcaaacgaaa tgtttgcggc tatgttatga cgttattcgg atgcgtatgt gttactgacg
    15121 accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt
    15181 aaattcagcc tgtactaccg gaacaaaaat catctgggcg atgcgttcgc caggttgaat
    15241 ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct gatagtcaga
    15301 atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca atccggagcg
    15361 cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc cggtcggaac
    15421 cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag
    15481 tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa cgcgcgggtc
    15541 cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg agtaataatt
    15601 ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc cagaaaaggt
    15661 gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga
    15721 tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca ttatttgttt
    15781 cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg cctgcgacga
    15841 tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc tgctttttga
    15901 ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcag ccgataaaaa
    15961 tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgcg gtcatcacat
    16021 caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt
    16081 tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg gagctgtgat
    16141 tagagatata acgcacggga tcgagcggtt cacgcgtcgg gccggcggta atcataatgt
    16201 tcagatgttt caggtcgttg acgggcgaaa aatgcgctac cgccatatcc acaatggtta
    16261 acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca ctgtctggcc
    16321 cccagatgag caaaccacgg gaagcaagca cctctaaatt atgctgcgtg gcagcggcac
    16381 ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta gccagacaaa
    16441 tcgtcgatac caggtcattc gccattccgg cagcaacacg ggcaatcaaa tctgccgtgg
    16501 caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc atagcggctt
    16561 cggctgccgg gtccagcaga ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa
    16621 gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg gccccgcgat
    16681 cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg ctaacgccga
    16741 gaacgatttt tttaccggcc aggctcatga tgatttttcc tgttgggtga caccagaagt
    16801 tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagaatca ctttgcgagg
    16861 cgctttccag gattgaaaac tggccgtcga tttaacggaa cggctatgac aggatgcgag
    16921 caccacaaag gaggtgaagg tgaaaaacaa ttcacagctg atgatgccgc gcgaaaaaat
    16981 gctgaagttt ggtattagcg ccttaacgga tgtcgagctg ctggcgctat ttctgcgtac
    17041 cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga atttcggctc
    17101 tctttatggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
    17161 ggcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
    17221 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg agggaatttt tacaaagcca
    17281 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactccc aacaccgggt
    17341 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
    17401 aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
    17461 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
    17521 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
    17581 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
    17641 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
    17701 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
    17761 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
    17821 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
    17881 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
    17941 ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
    18001 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
    18061 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
    18121 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
    18181 ctggaactga aaaaattcga tccagttgtt cgtcagcacg tgatctacaa agaagcgaaa
    18241 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
    18301 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
    18361 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
    18421 tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
    18481 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ttgctgctgg agctgcctga
    18541 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
    18601 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggtaaag tgctgcgcta
    18661 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcataatgt
    18721 gctggcccat cttggaccgg agccgctcag cgacgatttc aatggtgagt atctgcatca
    18781 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
    18841 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
    18901 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
    18961 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
    19021 taaaccgggc tatttcgcgc aggaattgca ggtttacggg cgaaaaggcg agccgtgtcg
    19081 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
    19141 gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata aacgctacgc
    19201 taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
    19261 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
    19321 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
    19381 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
    19441 ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca gatgcgcggt
    19501 tgcctgctgt gccagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
    19561 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
    19621 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
    19681 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
    19741 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
    19801 cggtgagtaa agaggacacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
    19861 cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca
    19921 ataccggaat agcgtgtgcg gcagcttcca gcggattatg cccaccacgt tcaaccagtg
    19981 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
    20041 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
    20101 ttagtccagc ctggcggaca agattaatcg catccgggaa gcgttctgga tgacggggta
    20161 ccaggatgag caataaattc gggaattgct gtaacaatgc ctgatgtgcg gcaatgacca
    20221 cgctctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
    20281 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
    20341 ggctaccggt aacggtcacc tgattatttt ttgcgcccag cgccacaaaa cgtgcaccat
    20401 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag caagcgacgg acgaatttac
    20461 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
    20521 gaattttacg tttgtgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
    20581 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
    20641 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
    20701 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
    20761 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
    20821 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
    20881 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
    20941 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
    21001 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
    21061 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
    21121 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
    21181 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
    21241 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
    21301 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
    21361 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
    21421 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
    21481 aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta
    21541 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
    21601 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
    21661 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
    21721 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg
    21781 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
    21841 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
    21901 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
    21961 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
    22021 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
    22081 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
    22141 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
    22201 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
    22261 gtccctggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
    22321 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
    22381 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
    22441 ttgtttatat aaatattttc cctttggcgg tttgcagcgt gattttatgc gtattgctca
    22501 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
    22561 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
    22621 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
    22681 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
    22741 gaaagtagcg caggaaaaag gctttttcta tcgcctgacg tcacgttatc gccattatgc
    22801 cgcctttgag cgggcaacct tcgaacaggg taagccgaca cagttgctga tgctgacaga
    22861 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
    22921 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagccaata gccgtgaaat
    22981 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
    23041 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
    23101 gcgccacaac acattgctat atgttgttgg gcaggataaa ccgcgaaaat ttgaggcgct
    23161 ggcagaaaaa cgcggcgtgc gcagtaatgt tcactttttc tcggggcgca atgatgtctc
    23221 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cggcgggaat
    23281 tgtgctgctg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
    23341 tgcgcattat attgtcgacg ctaattgcgg cgaggctatt gctgagccat tccgccagga
    23401 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
    23461 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
    23521 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
    23581 gcggcaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggtattt cgtgaactgg
    23641 aaactcgccg tactctgcgc tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
    23701 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
    23761 gcgctgaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
    23821 atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca tttattatta
    23881 ccgaagatct gacaccaacc ataagtcttg aagattacag tgctgactgg gcgactaacc
    23941 ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
    24001 tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
    24061 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
    24121 ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt
    24181 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg
    24241 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
    24301 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact tatttttgcc
    24361 aaaattttgg atacagaata aatatg
//
LOCUS       DAPPNJ010000058        24367 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000058 DAPPNJ010000000
VERSION     DAPPNJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.058"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            218..1654
                     /gene="mdtQ"
                     /locus_tag="RPM73_001888"
     CDS             218..1654
                     /gene="mdtQ"
                     /locus_tag="RPM73_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDX6520255.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1707..2468
                     /locus_tag="RPM73_001889"
     CDS             1707..2468
                     /locus_tag="RPM73_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6520256.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2598..3176)
                     /locus_tag="RPM73_001890"
     CDS             complement(2598..3176)
                     /locus_tag="RPM73_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6520257.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3346..3933
                     /locus_tag="RPM73_001891"
     CDS             3346..3933
                     /locus_tag="RPM73_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDX6520258.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4107..5039
                     /gene="pbpG"
                     /locus_tag="RPM73_001892"
     CDS             4107..5039
                     /gene="pbpG"
                     /locus_tag="RPM73_001892"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDX6520259.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5077..6792)
                     /gene="dld"
                     /locus_tag="RPM73_001893"
     CDS             complement(5077..6792)
                     /gene="dld"
                     /locus_tag="RPM73_001893"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDX6520260.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            6988..9285
                     /gene="bglX"
                     /locus_tag="RPM73_001894"
     CDS             6988..9285
                     /gene="bglX"
                     /locus_tag="RPM73_001894"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDX6520261.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9496..10413
                     /gene="osmF"
                     /locus_tag="RPM73_001895"
     CDS             9496..10413
                     /gene="osmF"
                     /locus_tag="RPM73_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDX6520262.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10420..11577
                     /gene="yehY"
                     /locus_tag="RPM73_001896"
     CDS             10420..11577
                     /gene="yehY"
                     /locus_tag="RPM73_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDX6520263.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11570..12496
                     /gene="yehX"
                     /locus_tag="RPM73_001897"
     CDS             11570..12496
                     /gene="yehX"
                     /locus_tag="RPM73_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDX6520264.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            12501..13232
                     /gene="yehW"
                     /locus_tag="RPM73_001898"
     CDS             12501..13232
                     /gene="yehW"
                     /locus_tag="RPM73_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDX6520265.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13213..13320)
                     /locus_tag="RPM73_001899"
     CDS             complement(13213..13320)
                     /locus_tag="RPM73_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDX6520266.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13380..14111)
                     /gene="mlrA"
                     /locus_tag="RPM73_001900"
     CDS             complement(13380..14111)
                     /gene="mlrA"
                     /locus_tag="RPM73_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDX6520267.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14333..16018
                     /gene="btsS"
                     /locus_tag="RPM73_001901"
     CDS             14333..16018
                     /gene="btsS"
                     /locus_tag="RPM73_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDX6520268.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16015..16734
                     /gene="yehT"
                     /locus_tag="RPM73_001902"
     CDS             16015..16734
                     /gene="yehT"
                     /locus_tag="RPM73_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDX6520269.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16781..17251
                     /locus_tag="RPM73_001903"
     CDS             16781..17251
                     /locus_tag="RPM73_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDX6520270.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17292..17753)
                     /locus_tag="RPM73_001904"
     CDS             complement(17292..17753)
                     /locus_tag="RPM73_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDX6520271.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17878..19878)
                     /locus_tag="RPM73_001905"
     CDS             complement(17878..19878)
                     /locus_tag="RPM73_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDX6520272.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19875..21011)
                     /locus_tag="RPM73_001906"
     CDS             complement(19875..21011)
                     /locus_tag="RPM73_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDX6520273.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21004..23283)
                     /locus_tag="RPM73_001907"
     CDS             complement(21004..23283)
                     /locus_tag="RPM73_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520274.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23294..>24367)
                     /locus_tag="RPM73_001908"
     CDS             complement(23294..>24367)
                     /locus_tag="RPM73_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDX6520275.1"
                     /translation="NHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAARAFILG
                     DSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISGDAGLT
                     IQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEV
                     QDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFE
                     TVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMD
                     TLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYF
                     EQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5655 a         6247 c         6674 g         5791 t
ORIGIN      
        1 tcctttattt gtgagtgctg aataagtata ggaaaaaacg aagccctttg ccgttatcca
       61 taaacgatta agataaatcg gaagatatca ttcacttagt gtatattttt ctggatatgt
      121 cgttttattg ctactgatgt attgccacgc gaaactaaaa aatatatcgc aacacacggt
      181 aatctccgcg caccttttct tacctgatta ttcccccatg aatcgtgatt ccttttatcc
      241 cgccatcgcc tgttttccgc tgttactgat gctggccggg tgtgcgccta tgcatgaaac
      301 ccgccaggca ttaagccagc aaacgcccgc tgcacaagtt gacaccgcat tacccacggc
      361 gctgaaaaat ggttggccag acagccaatg gtggctggag tatcacgata atcaactcac
      421 ttccttaatt aacaatgcac tacagaacgc accggatatg caggtggctg aacagcgcat
      481 tcagctagct gaagcccagg cgaaggcaat tgctactcag gatggtccgc agatcgactt
      541 ttctgcggat atggagcggc aaaaaatgtc ggcagaaggc ttaatggggc cgtttgctct
      601 gaacgatccg gccgcaggta cgaccggtcc gtggtacacc aacggaactt ttggcttaac
      661 ggcgggctgg catctcgata tctggggaaa gaatcgggcg gaggttactg cccgcctggg
      721 tacggttaaa gcacgggcgg cggaacgcga gcaaacccgc caattgctgg ctggcagcgt
      781 agcccgcctg tactgggagt ggcaaaccca ggcggcgtta aacacggtct tgcagcaaat
      841 agaaaaagag cagaacacca ttatcgcgac cgatcgccag ctatatcaga acgggattac
      901 ttcttcagtt gaaggtgtgg aaaccgatat taatgccagc aaaacccggc agcagctcaa
      961 cgatgtcgcg gggaaaatga aaattattga ggcacggtta agcgcactta caaataacca
     1021 gacaaagtca ttgaagctta aaccggtcgc gttgccgaaa gtggcaagcc agcttcctga
     1081 tgaactgggg tactccttac tggcccggcg ggcagatttg caggcggcgc actggtacgt
     1141 tgagtcatcg ctaagcacca ttgatgcggc aaaagcggca ttttatcctg acatcaacct
     1201 gatggccttc ctgcaacagg atgcgttgca cttaagcgat ctgttccgtc attccgcgca
     1261 gcaaatgggc gttacggcag gcctgacgct acccattttc gatagtggtc gtcttaacgc
     1321 caatctcgat atcgcaaaag ccgaaagcaa cttgtctatc gccagctaca acaaagcggt
     1381 ggttgaagcg gtgaatgacg tggcgcgggc agccagtcag gttcagacac ttgcggagaa
     1441 aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtggtaggtc ttgcgcaggc
     1501 gcgctttaac gcgggcatca ttgctggttc ccgcgtcagc gaagccagaa tccccgcgct
     1561 gcgtgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgctt ccattcaact
     1621 cactggtgcg ttgggcgggg gttacaaacg ctgatgatat gcgccttcta tacttaacgt
     1681 ttattcagcg ttaagtggag aactcgatgg cacaggttgc gattattacc gcctccgatt
     1741 cggggatcgg caaagagtgc gcgttattac tggcgcagca ggggtttgat attggcatta
     1801 cctggcactc agatgaagaa ggggcaaaag ataccgcgcg tgaggtagtt agccacggcg
     1861 tacgtgcgga gattgtgcag ctggatctcg gtaaactgcc agaaggtgca caggcgctgg
     1921 agaaactcat tcaacggctg gggcgtattg atgtgctggt gaataatgcg ggcgcaatga
     1981 ccaaagcgcc gtttcttgat atggctttcg atgagtggcg caagattttt accgttgatg
     2041 tcgatggtgc atttttatgc tcacaaattg cggcccgcca gatggtgaaa caagggcagg
     2101 gtggtcgcat catcaacatt acgtcggtac atgaacatac gccgctgccg gatgccagcg
     2161 cctacacagc cgctaaacat gcgctcggcg ggttaaccaa agcaatggcg ctggagttgg
     2221 tcaggcataa gattttggtg aacgcagtcg cacctggggc gatcgctacg ccaatgaacg
     2281 gcatggatga tagcgacgtg aagcccgacg cggagccttc gattcctctg cggcgttttg
     2341 gcgcaacgta tgagattgcc agcctggtgg cgtggctttg ttcggagggc gcaaattaca
     2401 ccaccgggca gtcgttgata gtggatggcg gctttatgtt ggcgaatccg cagttcaacc
     2461 cagaatagcg cagtgaggtg tgtggatatt tcaatgggta aaattttggt aggcatgata
     2521 agacgcacaa gcgccgcatc aggcatggtg caccggatgt cggatgcggc gcgaacgcct
     2581 tatccagggc aatggtttta cgcctgatta tccggctttt tcttcccgcg tcgtttcagc
     2641 caccagcgca cgcctaccac cagaaccacg accagaatca accagaccca gtgcttcaaa
     2701 tgctgatcga gattgtgcaa ccacggcgca atcacctgac caccagcgta accgatagtg
     2761 gtaaaaatca acgcccaggc aaatgcgccg agaatattaa gcggcagaaa gattttcggc
     2821 ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc gaaagccata cataaagcgc
     2881 gtaccaatga caaacagata cggatggcgt tggataagtt tctgcgcccg ctcaatttta
     2941 tcctgatgtt tcgagaaacg gcgtaacagc ttgccgccaa accgccgccc gcacagataa
     3001 agcacctggt cgccaatcat gccgccaagc gccacagaaa gtaccaccag cgggaacttt
     3061 aatagcccct gatgcgccgc aacgcctccc agcaaagtca cggtttcacc ttccgccagg
     3121 ctaccgatca ccagcgcggc ataaccatat tgtgagatga gtgtattgag atccataggc
     3181 cagtctgtct ccctttggtt gcctgtaagc atacaccctg gaaatcattc acgctgaaaa
     3241 aggacgtaca tcagacggtg caaattacat gagcgaacaa aaaataatca caagctgact
     3301 tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag ccatgtctgg
     3361 ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa cgaaacgatt
     3421 tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat ttgcgccttc
     3481 attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa gttatcctgg
     3541 tttaccggac tggcgctggc tgtcctgttt tatggcgtta tgctggctgg ggttgcggta
     3601 atggggcggg tcatctggtg gatggcgcgt aattatccgc agcgtccgtc acttgcgcac
     3661 tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct ggtggcgctt
     3721 tatccgctgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta caccggctac
     3781 ctgctttacc ttggtattcc gagcttcctg aatatcaaca aagaggaagg ccttagcttc
     3841 tccagttcga cgctggcgat tggcgtgctg gtactggaag tgctgctcgc cctaaccgtt
     3901 atactatggg gctacgggta tcgcctgttc tagaaagttc tttgacattg cattgctggc
     3961 gtaaatgtca gcaatgcagc atttcctcac gattctcctt tgacgatcgc actttttgct
     4021 cgttatgatg gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc aacccgtgcg
     4081 cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta tttagcctgg
     4141 ccctgatgct ggctgtgcct ttggcaccgc aggccgttgc gaaaacggca gccgctacca
     4201 ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg aataccaaca
     4261 aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc agcaaattaa
     4321 tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta aaagtggata
     4381 tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaat agcgaaatca
     4441 gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg gcggcaagcc
     4501 ttgcgcacca ttatcccggt ggttacaaag cctttattaa ggcaatgaat gcgaaagcga
     4561 aatcgctcgg aatgaacaac acgcgctttg ttgaacctac cggattgtcg gtgcataacg
     4621 tttcaactgc ccgtgactta accaaactgc tcattgccag caaacagtat ccgttgatcg
     4681 ggcagttaag tactacccgg gaagatatgg caaccttctc taacccgacg tatacgctgc
     4741 cgttccgcaa tactaatcat ctggtgtatc gcgataactg gaatattcag ttaaccaaaa
     4801 ccggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc aataataaac
     4861 cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc gatgccagcc
     4921 gcctgcgtac ctggattgaa accggtaaag tgatgcctgt gccggcagca gcgttgagct
     4981 ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag aacgattaag
     5041 tgaattcgga tggcgatact ctgccatccg taatttttac tccacttcct gccagttttt
     5101 ccgtttactg gttttaccga ttcccggatt catgctgttg gtcggatcgt tctcgcgata
     5161 gaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgct cggcagggta
     5221 ttgcgcgccg cgctgctgta gcagttccag catctgttct tttaacgcat gcacatccac
     5281 gccttttttc actatgtaat cctgatggaa gacatagcac ataaaatggc cgtaatagag
     5341 cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact cggtgtcgtt
     5401 acgccgcaga gcgatatcca acgccagaat gtcttcgact tcatcggaat gcaccgcctg
     5461 ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc tgccttcctc
     5521 cggcgtacag acaaagaaat cgccttcggc ctgtttgaaa taatccacca accacgattt
     5581 ggcttcgccc acgccatcgc ccgccatttt taacagcaga tgatgctcgt atttatcgcg
     5641 ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt tttgcatcgc
     5701 acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat cggtgcgtcc
     5761 cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca tcaggaaggt
     5821 gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt ccccggcaac
     5881 cggcagattt tcgaagttag ccagaatatg acggcggatt tcggtcagca cttccggctg
     5941 gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat ctagacgtac
     6001 cgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat ccggatcggc
     6061 gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat agtcataatc
     6121 gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcat catccagctt
     6181 gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca ccagcgtcag
     6241 tttgccgtct tcatttatac gcgcgaataa cgacatttcg gtatacgccg ggccacgttg
     6301 caccagcgag ccgccggagt tgttacaaat accgccgata accgatgcgc ctatacacga
     6361 cgatccaatc accgagtgcg gttcgcgtcc cagcggtttg agggcttttt ccagcgaata
     6421 gagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccaagaacgt gcagcttgtc
     6481 gagacgcagg gtgctgatga tcacgatatc gcgatcatag tcgttaccgt ttggcgtcga
     6541 tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg cggtgacaca
     6601 ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag ccagcgcgtc
     6661 gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat cggtgagcag
     6721 gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat cagttgttgt
     6781 catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact agaatagcgc
     6841 cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac tgagcattct
     6901 ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt cgctgcctcg
     6961 tcgggcaaca gaggaagaaa aatccatatg aaatggctat gttcagtagg aatcgcggtg
     7021 agtctggccc tgcagccagc actggcggat gatttattcg gcaaccatcc attaacgccc
     7081 gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga tgagaaaatt
     7141 ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat ccgcgagatg
     7201 atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga tatccgcgcc
     7261 atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt tgcttacgac
     7321 gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc gtcttttaac
     7381 ctcgatgcag tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga tgatggcctg
     7441 aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg acgtgcttcc
     7501 gaaggttttg gcgaagatac gtatctcacc tcaacaatgg gtaaaaccat ggtggaagcg
     7561 atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa acactttgcc
     7621 gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag tccgcagcgc
     7681 ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag cggcgcggtg
     7741 atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcctg gctgctgaaa
     7801 gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca cggtgcaatc
     7861 aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg cgtggcgctg
     7921 aaatccggaa tcaacatgag tatgagcgac gagtattact cgaagtatct gcctgggttg
     7981 atcaaatccg gcaaagtgac gatggaagag ctggacgatg ctgcccgcca tgtactgaac
     8041 gttaaatatg atatgggatt gtttaacgac ccatacagcc atttggggcc gaaagagtct
     8101 gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg tgaagtggcg
     8161 cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa aaaatcggcc
     8221 accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg cagctggtcc
     8281 gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggatcaaaaa ttccgtcggt
     8341 gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa aggcattatc
     8401 gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc gcaagagatg
     8461 attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt agtcggtgaa
     8521 gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc gcaaagccaa
     8581 cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt gctgatgaac
     8641 gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct ggaaacctgg
     8701 tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga ttacaacccg
     8761 tccggcaagc tgccaatgtc cttcccgcgt tctgtcgggc agatcccggt gtactacagc
     8821 catctgaata ccggtcgccc gtataatgcc gacaagccga acaaatacac ttcgcgttat
     8881 tttgatgaag ctaacggggc gttgtatccg ttcggctatg ggctgagcta caccactttc
     8941 accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa agtgactgcc
     9001 agcgtgcagg tgacgaacac cggtaagcgc gagggggcca cggtagtgca gatgtacttg
     9061 caggatgtga cggcttccat gagtcgccct gtgaaacagc tgaaaggctt tgagaaaatc
     9121 accctgaaac cgggcgaaac tcagactgtc agcttcccga tcgatattga ggcgctgaag
     9181 ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt atttatcggc
     9241 actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt tagtaagggc
     9301 gcgacgtttg cgccctttgt aggccggata aggcgctcgc gccgcatccg gcaggaaacg
     9361 ccggaccgaa tatgcctctt aagtatttat ctgattctgc aactgaatct ttccgctcaa
     9421 gctaaccccg ccattatcaa ctatgctttt ctcttaattc gctgaaaaca acagtaaaag
     9481 aatgaggaaa gcatcatgcc actcttaaag ctctgggcag gttcactggt tatgttggca
     9541 gccgtgagcc tgccgctaca ggcggcttcc cccgttaaag tcggttcaaa aatcgatacc
     9601 gaaggtgcgc tactcggcaa tatcattttg caggtgctgg aaagccacgg cgtaccgacg
     9661 gtgaataaag tgcaactcgg aacgactcct gtggtgcgcg gggcgattac ttccggtgaa
     9721 ctggatatct atccggaata taccggcaat ggcgctttct tctttaaaga tgaaaacgat
     9781 gcagcgtgga aaaacgcgca gcaaggttac gagaaagtca aaaagctcga ttcggagcac
     9841 aacaagttaa tctggctgac gcccgcgcct gcaaataaca cctggactat cgccgtgcgt
     9901 caggatgtgg cagagaaaaa taaactcact tcgcttgccg acctgagtcg ttatctgcaa
     9961 gagggcggca ccttcaaact ggcagcctcg gcagagttta tcgaacgcgc cgatgcgtta
    10021 cccgcgtttg aaaaagccta tggctttaag ctcggtcagg atcagttgct gtcactggct
    10081 ggtggcgaca cggcggtgac gatcaaagcc gctgcccagc aaacctctgg cgttaatgct
    10141 gcaatggctt acggcactga cggcccggta gcggcgctgg ggctgcaaac cttaagcgat
    10201 ccgcaaggcg tgcaacctat ctatgcgcct gcaccagtgg tgcgtgaatc ggtgttgaag
    10261 gagtatccgc aaatggcaca gtggctacag ccagtcttcg ccagcctcga tgcaaaaaca
    10321 ttgcagcaac tgaatgccag cattgcagtt gaaggactgg atgccaaaaa agtggctgcc
    10381 gactacctga aacaaaaagg gtggacgaag taatttcccg tgacttatct acgtattaat
    10441 cctgttctgg cgctgctgct gttgctgacg gtaatcgcag cggcgctgcc gtttatcagt
    10501 tacgcgccta atcgtttagt ttcgggtgag gggcgtcatc tctggcagct gtggccgcaa
    10561 acgatctgga tgctggtggg cgttggttgc gcctggctga cggcctgttt tattcccggt
    10621 aaaaaaggca gcatttgtgc actcattctg gcgcaattcg tcttcgtatt gctggtgtgg
    10681 ggcgcgggaa aggcggcgac acaactggcg caaaatggca gtgcgctggc gcgtaccagc
    10741 ctcggcagtg gtttctggct ggctgcggcg ctggcattgc tggcctgtag cgatgccatc
    10801 cgccgaatct ccacgcatcc gctgtggcgc tggttgttgc atatgcagat tgccattatt
    10861 ccgctgtggt tgctgtactc cggcacgctt aacgatctct cactaatgaa agaatacgcc
    10921 aaccgtcagg atgtgtttga cgacgcgctg gcacaacatc tgacgttgct gtttggtgcg
    10981 gtgctgcctg cgttagtgat tggtgtgccg ttgggcatct ggtgctactt ttccactgct
    11041 cggcaggggg caattttttc tctgctcaat gtcattcaga ccgtgccttc ggtggcgctc
    11101 tttggcctgt tgattgcgcc gcttgccgcg ctggtgacgg cctttccgtg gctggggaag
    11161 ctcggcatag caggaaccgg aatgacaccc gcactgattg cgctggtgct ctatgccttg
    11221 ctgccgctgg tgcgcggcgt ggtagtcggt ttgaaccaga tcccgcgcga tgtgctggag
    11281 agcgccagag cgatgggcat gagcggggcg cagcgattcc tgcatgttca gttaccactg
    11341 gcgttaccgg tatttctgcg cagcctgcgg gtggtgatgg tgcaaactgt aggtatggcg
    11401 gtgattgcgg cgttaatcgg cgcaggcggt tttggtgcgc tggttttcca ggggctgcta
    11461 agcagcgcca ttgatttagt gttgctgggg gtgatcccgg taattgttct ggcggtgctg
    11521 atcgatgccc tgttcgattt gcttatcgca ctgctgaagg tgaaacgtaa tgattgaatt
    11581 tagccatgtc agcaaactgt ttggcgcaca aaaagccgtt aacgatctta atctcaattt
    11641 tcaggaaggg agtttttcgg tgctgattgg cacatctggc tccggcaaat ccaccacctt
    11701 gaaaatgatt aaccgcctgg tggaacatga cagcggagag atccgctttg ccggagaaga
    11761 aattcgctcg ctgccagtac tggagttgcg ccgccggatg ggctatgcca ttcaatctat
    11821 tggcctattc ccccactgga gcgtggcgca aaacatcgct accgtgccgc aattacaaaa
    11881 atggtcgcgg gcacggattg acgatcgtat cgacgaatta atgacgctac tggggctgga
    11941 gtcaaatttg cgtgagcgtt atccgcatca gctttccggt ggtcagcagc aacgtgtggg
    12001 agtggcgcgc gcactggctg ccgatccgca agtcttactg atggatgaac cttttggcgc
    12061 actggacccg gtaacgcgcg gcgcgttgca acaagagatg acgcgcattc accgtttgct
    12121 ggggcgcact attgtgctgg tcactcatga tattgatgag gcgctacggc tggctgaaca
    12181 tctggtattg atggatcacg gtgaagtggt gcagcagggc aatccgctga cgatgctgac
    12241 tcgtccggcg aatgattttg tccgccagtt ttttggacgt agcgaactgg gtgtgcgcct
    12301 gctttcgtta cgtagtgtgg cggattacgt gcgtcgcgaa gaacgggcag aaggtgaggc
    12361 actggcagaa gagatgacgc tacgcgatgc gctctccctg tttgtcgcgc ggggatgcga
    12421 ggtgctgccg gtggtgaaca cgcagggcca gccttgcggc acgctgcatt ttcaggatct
    12481 gctggaggag gcgtaagcgt atgaagatgt tgcgcgatcc gctgttctgg ctcattgctt
    12541 tgtttgtggc actgattttc tggctgcctt acagccagcc gctgtttgct gccttgttcc
    12601 cacaactgcc acgacccgtt tatcagcaag aaagttttgc agctctggca ctggctcatt
    12661 tctggctggt gggaatttcg agtttgtttg cggtgatcat tggcactggt gccggaattg
    12721 ctgtcactcg cccgtggggc gcggaatttc gcccactggt ggaaactatt gccgccgttg
    12781 gacagacttt tccgcctgtc gcagtgctgg cgattgccgt tccggtgatc ggctttggtc
    12841 tgcaaccagc gattatcgcc ttgatccttt acggtgtgct gcccgtcctg caggcgacac
    12901 ttgccgggct gggagcgatt gatgccagcg tgacagaagt tgcgaaaggt atgggaatga
    12961 gtcgtggtca gcgactgcgt aaggtcgagc taccgctggc ggctccggtg attctggcgg
    13021 gcgtgcgaac ttcggtgatt atcaacattg gtacggcgac gatcgcctca acggtagggg
    13081 ccagcacgct gggtacgccc atcatcatcg ggcttagcgg atttaatacc gcgtatgtga
    13141 tccagggggc gttactggtg gcactggcgg cgatcatcgc agaccgcctg tttgaaaggc
    13201 tggtgcaggc gcttagccag cacgcaaaat aaaggtataa cctgcgagca tgacgccacc
    13261 aattccgccg aacgccataa acaggaacag ggcgatgacc ccaattttag ctatgcgcat
    13321 attgcactcc ttatgttaac gaaaggattg tacagtaaag cgcatttgtt aacgaatcat
    13381 taaatgccga gtgggaaaat atcatggcct tgttcttgcc aactggtgag ttgctgctgt
    13441 tgggcggagg ttcgattttc accgcaccac accagcaatg tacggccttc gaatagttca
    13501 gggcgtagtt gattgagcga gtgggcgagg acatcaatgc gccatccttg ttgactggca
    13561 atccagccct ccagccacag acgggtggta tcctgaatat tccagccaac caccagcgca
    13621 tctttaccct gttttttacg tgccgaagcc agacaaatgg cgatgtagtt gatcagtacg
    13681 ccgtcgagga tcgccagcag cgcctggaga gtcggttgtt ggcactgaag ccgtcggcgc
    13741 agaggaataa acagatgtgt ggtgagtgtc tgggcggggt aatcctgacc gcgctctttg
    13801 atccacgttc gcaggctatg cagattgccg ctttgcaggt aggtcagtaa tgtttcttgc
    13861 tgatcgcgcc agccgttctg cacatcaaca ttttcattac tgagcagcat tttaactttg
    13921 ctgacctgca cgccgttgtc gatccagcgt ttgatctcgc ggatacggtc aatatcggca
    13981 tcgttgaaca gtcgatgacc gccgtctgtc cgttgcggtt tcagcaaccc gtaacgcctc
    14041 tgccacgcgc gtaacgtgac agggttaata tcacaaagca acgccacttc accaattgtg
    14101 taaagcgcca tcgtctcacc cttgctcgcg aggtcccggt ttaactttag acgcagtttt
    14161 gcgaaccagg tagttttgcc cgttttttgt gcatctatag ggtgatttta tttttgccag
    14221 gcgattttga gtgatcgtac tcacgaattc tcatttttct gcaagagttc aaagaaagtt
    14281 aaacgcaggc aatgtatgtt acgcgtttta aagggaagtg tggtttgcgg gtatgtacga
    14341 ttttaatctg gtgttgctgc tgcttcagca gatgtgcgtt tttttagtca ttgcgtggtt
    14401 aatgagtaaa acgccattat tcataccgtt aatgcaggtc acggttcgtc tgccgcataa
    14461 atttctctgc tacatcgtct tttccatctt ctgcatcatg ggcacctggt ttgggttgca
    14521 cattgacgat tctattgcca atacccgtgc gataggcgcg gtaatgggcg gcttactcgg
    14581 cggtccggtc gtcggtgggc tggttggtct gaccggcggc ttacatcgat attcgatggg
    14641 gggcatgacc gcgctaagtt gcatgatctc aaccatcgtt gaaggattgc tcggcggcct
    14701 ggtacacagc atcctgatcc gtcgcgggcg cactgataaa gtctttaacc ccattaccgc
    14761 cggtgccgtc acgttcgtcg ctgaaatggt gcaaatgctg atcatcctgg cgatcgcccg
    14821 accttatgaa gatgcggtgc gtctggtgag taatattgct gcgccaatga tggtcaccaa
    14881 taccgtcggc gcggcgctgt ttatgcgtat attgctcgat aaacgcgcga tgtttgaaaa
    14941 atacacttcg gctttttctg ccactgcgct gaaagtggca gcctcgacgg aaggcatttt
    15001 gcgacagggg tttaacgaag tgaacagcat gaaagtggca caggtgctgt atcaggagct
    15061 ggatattggt gcagtcgcga ttaccgatcg agagaaattg ctggccttta ccggaattgg
    15121 tgacgatcac catttacccg gcaaaccgat ttcttcgact tacaccttaa aagcgattga
    15181 aaccggtgaa gtggtctacg ctgatggcaa cgaagtacct tatcgttgct ctttgcatcc
    15241 gcaatgcaaa ctggggtcga cgctggtaat tccgttgcgt ggtgaaaatc agcgggtgat
    15301 gggcaccatc aaattgtatg aagccaaaaa ccgtttattc agttcaatca accgcacgct
    15361 gggcgagggg attgcgcaac tgctttcggc gcagatcctc gccgggcaat atgagcggca
    15421 aaaagcgatg ctcacccagt cagagatcaa actgcttcac gcccaggtga acccccattt
    15481 tttgtttaat gcgcttaaca ccattaaagc ggtgatccgt cgcgacagcg aacaggccag
    15541 ccagctggtg cagtatcttt ccactttttt ccgcaaaaac ttaaagcggc cttcggagtt
    15601 tgttactctc gccgacgaaa ttgaacatgt gaacgcttat ctgcaaattg aaaaggcgcg
    15661 cttccagtcg cggttgcagg tcaacattgc tattccgcaa gaattatccc agcagcaatt
    15721 gcccgcgttt accctgcaac cgatagtgga aaacgccatt aaacatggga catcacaact
    15781 gctggataca gggcgagtgg caatcagcgc ccgacgtgag gggcaacatt tgatgctgga
    15841 gatcgaagac aatgccggtt tgtatcaacc ggtaaccaat gccagtgggc tggggatgaa
    15901 tctggtggat aagcgtttac gtgaacggtt tggcgatgac tatggaataa gcgtcgcctg
    15961 tgagcctgat agttacaccc gaataacgtt acgactacca tggagggacg aggcatgatt
    16021 aaagtcttaa ttgtcgatga tgaaccgtta gcacgggaga acctgcgtgt atttttgcag
    16081 gagcagagcg atattgaaat cgttggagag tgttcaaacg ccgtggaagg gatcggcgcg
    16141 gtgcataaac tgcgcccgga tgtgctgttt ctcgatatcc agatgccgcg catcagtggt
    16201 ctggaaatgg tggggatgct tgacccggaa catcgcccgt atattgtttt tctcactgcg
    16261 tttgacgaat acgctattaa agcctttgaa gaacatgcct ttgattatct gctgaagcca
    16321 attgatgaag cgcgactgga gaaaacgctg gcgcgattgc gtcaggagcg cagcaagcag
    16381 gatgtttcgc tgttaccgga aaatcaacag gcgctgaaat ttatcccttg tacggggcat
    16441 agtcggattt atttgctgca aatgaaagat gtggcatttg tcagcagtcg gatgagcggt
    16501 gtctacgtta ccagccacga agggaaagag ggctttaccg aattgacatt acgtaccctg
    16561 gaaagtcgta caccactact gcgctgccat cgtcagtatc tggttaacct cgcgcattta
    16621 caggagattc gtctggaaga taacggccag gccgagttga ttttgcgtaa tggcttaacc
    16681 gtgccggtca gccgccgtta tctgaaaagc ttaaaagagg cgattggcct gtaaaagact
    16741 gctaaaatgg ctttttgcct catcaacacc tgaaggcctc atgctaagta acgatattct
    16801 gcgcagcgtg cgctacattt tgaaagccaa taataatgac ctggtgcgta ttctggcgct
    16861 gggtaatgtc gaagccaccg cggaacagat cgccgtctgg ctacgtaaag aagacgaaga
    16921 gggttttcag cgttgtccgg acattgtttt gtcgtcattc ctcaatggcc tgatttatga
    16981 aaaacgcggc aaggatgagt ctgctccggc actggagccg gaacgtcgca ttaataacaa
    17041 catcgtgctg aaaaaattac gcatcgcgtt ttcgctgaaa accgatgaca ttctggctat
    17101 cctcaccgaa cagcagttcc gcgtttcgat gccggaaatt acagcgatga tgcgcgcacc
    17161 ggatcataaa aacttccgcg aatgcggcga tcaattttta cgttattttc tgcgtggact
    17221 ggcagcgcgc cagcatgtga agaaaagcta agacgggtat ggcggccatg cgaaacatgg
    17281 ccgccgacag attatttcac ttctttaaaa ccagcggctt tcatcaccag ttccatttgc
    17341 gccatagtga tacctttttt ggcatcttca gcagaaacgt tgattcctga aataccctgc
    17401 agggctttaa aatccacttt ttccatatcg atagtcacgt tttcctgcgc gtaggtatct
    17461 gtataggtta atttttcttc aacacccgcg atgtttttgt atttggcgct taacggctca
    17521 agtgtcttgg cagcgtcttc tttggtggtt gcaccaatag aagcaaattg aattttggtt
    17581 tcagaagatt gcttaagcac cttgtcacct ttgtagacat aggtaatggc aatttcagtg
    17641 ccgttcagat tggcgctgaa tttcttcgat tcttctttgt caccgcagcc agcaagagag
    17701 aaaaccagaa cagatgcaac aacgagggaa aacagcttat tgaaagcctt catgtaaaac
    17761 tccattttat ttaatcaaga aactggtgac tctcaccagg ggctatatag aatatgccta
    17821 ataccgtgac gtgagcagtc cggaactgga gtagaactct tagtaaaaag cactatttca
    17881 tccttgttgc tgaagcatgg ggaataattg ttcgcaaagt aaaacaccgt tattcattgc
    17941 ttctacccgt gcctcgcttt ctgtattacg aaattgtccc aacacatgtg ccagccgata
    18001 aaaaccgacc gcggagaggt cattcgccag caactctgcc tgactaatag cgctctgttc
    18061 ctgatagcgc cagccgttat ggagcagttg aataagtaac gcctggcagc gcatcagcaa
    18121 ctgatgagcg gtagacggca caggcaaaac gctggcagaa ggtagcggtg ccacaggcgc
    18181 agtttctgcg tccagcgccc aggcgcgggt ttttgtcatc atcacccgtg gttccagtgt
    18241 caattgccca tcaacaaaac tgacaaagcc agaaaccaga ctcacggggt cgtctgtttg
    18301 ttgcaaaagc gccgccatgc gttcaacggc aaaaggagaa caggcagatg ccggaaggga
    18361 taacgtcagg agattatctt caccttcgcc gctgattacc tgcgcatcca gcgtctggcg
    18421 gctgctatcc caaccgagcg aaatacactc agcgacaggc agaataaata agttatcgac
    18481 ctgattaaga ggccgtatgc aggcgggggg acgctggcgt aaatattccc gcaaagccac
    18541 aatgcccggc tggcgtaacg gcgcgctcaa catttgccag gcatcaggcg acagcggcac
    18601 aacgctgctt aagcggttgc gggtagctaa cagcagctcg ccatcggcac tgcgttttgc
    18661 tgcttgtgaa acaatttgcc cacccgccag tgcgccagcc tgaaaactaa acagccgacg
    18721 cgtagctgcc ggtgagtttt cctgttcact tcgcggccaa ctgcgcgaaa ggtgcaaaat
    18781 actgccggtg tcgggatcgg taaaccagat gcgtaaacca taatgctcaa tatcctgcca
    18841 gcaacgcata cctaaagaca ccagccgcag atgatcaagc tttgcttctc cggcaatgcc
    18901 agagccaacg accgtgcgcc acggcacagg aggaacttca ccaacactgt cgcgccgggc
    18961 catctcttgt gcgcaattta atcgactgtt taatgccgca agctgacgta agcattctcc
    19021 ggcatgatag tggctggcgc gagtgtggaa ggcatcaacg cttgcccgta gctgtcgtag
    19081 tgattcactc acccagcgcc agttgcaggc ctctgccgcc tgcaatgcgc ggttgaatgc
    19141 tgcctcgtag tgaataagcg gctggctgat gccgccaagc cataatgcct ggcttaattg
    19201 ctgaacatat tgacgacacg tttggccttc ttcgctggca aacggatcgt cagatgatgt
    19261 gacgtgttcg ctgcgcatct gccagattaa atggttaaat tctgcttgct gcgctttggc
    19321 ctcgacgaag gcctgcacag ccagtacgac atgttcgcaa agtgtgcctt caatacaatc
    19381 acaacgggcg aaacgaatac tgctacggga ataaaaacgc acatcgctca tcggtaagcg
    19441 ggcagaggga atttcgcccg gcgcacagaa caactcaatg gtgatgcctt tagcgaccag
    19501 cgcctgcgcg cgtttgcggg tggcatcggg cagggtagcc agttcttcca gccagattgc
    19561 cggatcccac tcttcttctt tttccgtggg ctggacggtg gcacaaagtc gttgataact
    19621 taacaccagc atcacgcgat gacggcacat gccgctggcc ccgcagctgc actgagcctc
    19681 tttcagtgcc tggctgttcg ccagctgggt acggacaccg tcactgaagg tggcgattaa
    19741 agcgccgttc tcatggctga tttccgggac gttgccattt tccagttcct taagactgcg
    19801 cttaacaaaa ccggcattgc ttaacgccgt cagtgcctgc ggtgtcagtt ctaataattc
    19861 cggacgtagt gaattcatga ctgaagattc tccgcaagcc atgatgccag ctcgcccggc
    19921 gtcatggcgg ctatttgtgc gccgacatta accagcgcct gggccgtatc gcggtcatag
    19981 caaggcgttg cggtgctatc gagcgctgcc agtcccagca ctttgatgcc gctctggaca
    20041 cactttttca cctgatgcgt cagcaatgat gatgaacccc cttcgtaaaa atcgctcacg
    20101 aggataatga cgctttttgc tggttgttca ataagttgcc gaccatactc cacggcactg
    20161 gcgatattgg tcccgccgcc caactgtact ttcattaata actctaccgg atcggcaacg
    20221 tctgccgtga gatcaaccac gcttgtgtca aacgccacca gatgggtacg aatgccgggt
    20281 aactgccaca aacaggccgc catcaccgca gagtggatca ccgagtccac catcgatccg
    20341 ctttgatcaa ccagtaagac cagttgccat tgttcgcttt ggcgtttaat gcggctgtta
    20401 aagcgggggg attcgatata caacttgccg tgttgcgggt gccagtgttg caggttggcg
    20461 cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa ttaatgagcg gcgacggcga
    20521 tcgcggacac cagaaaaagc ctgacgaact tcctttgcca gtcgcgccat aatttcttca
    20581 acaacctggc gcactatctg gcgggcggca gccagtactt cgggattcat cagatgtttg
    20641 gtgtgcaaaa cggcgcgtag caggctttcc gaaggctgca tacgttccag cacgtcgaga
    20701 tttgtcacca catcttcaat gccgtagcgc agtacggcat cgctttccag ccgctcaatc
    20761 acctgttgcg gaaacagcgt gtggatactg ttgatccact caggagtggt gagatttgag
    20821 ccacctaatc caccagagcg ttcaccacgc tggagccgtt caggatcgcg cccatacagc
    20881 cactccagcg cgtgatctat ctgccgggcg ttgtcatcca gcccacaaag cgtcgtttct
    20941 gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag aagatcgttc
    21001 agttcagaca tggaaaactc cccagtgttg cagcgatgcc agtgcctgct gttcgagttg
    21061 ttgatgatgt gcaatggctt gcggtggaca atgcaacggc atttgcagtg ccgaaacggg
    21121 aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgttccgc gttctcgtgg
    21181 cggtagccag gccatcgccg cgcgtaaatc gggcagggca ttgataaaat tggcatcact
    21241 cagttgattc aaatggctgc tgaaaccggc gataaatgca ggttgacagg ccagttgatg
    21301 acgggctaat gccagcaaac cgtgcagcgc ctcgccggat tgtgccgggg ataactgcgc
    21361 cagcatcgtc agcgccgctt cggcactggc gttgggatgt tccagacgca ttagtgcgcc
    21421 aagagccgcg ccgcgatcca gcgccggggc atgaatcgcc tgactgcgtc gctccagtaa
    21481 ggctaccgcc gcagaaagcg aaacgccgga taaattaacg ccgctatgta gatcgcgcag
    21541 aatatggcaa agcgcttgcc agctatgcag gtgagcgtga aactcctttt catccggtct
    21601 gccgttagat tcacacagcc acagcgtgcg atcgatagcc gcgcataacg tcgtttgtaa
    21661 tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat atagcacttc
    21721 cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg tgagttgctc
    21781 cagtaattgt tgactgaagg acgccagacc cgctaacgcc gcctggctaa gacatgtggt
    21841 gatactgccg attccacccg cgtccagcat atcggcttct aatttatggc gtgcggcttc
    21901 ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc gcatgctggc taagcgggcg
    21961 ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc agtgtacttc cctgctggcg
    22021 tacaatccca gggatctcca gtattgccag ccgatgtaac acctgacttt gcgccagccc
    22081 atcgggggta aagcgattta aggtaagctc cgccggaaga gagattgcgg tacgttccag
    22141 ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg gaaggggcaa gtttaccgaa
    22201 tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat cgggatgaat
    22261 aacgccgcga tagctccacg gcaacggtgc attcagggct tctttaatca acgagcctgc
    22321 tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact gtgccagtgc
    22381 cattgcatgc agatgtgcgg cagccatatc cgcggtcgaa gcaggcaagt tgtgctggcg
    22441 caaacgggtg agaaccgttt ttagcagttg ttcaccggcc tgctgtaagc cccactgcca
    22501 gcaccagttt tgccagaccg gggcaggcat accggaaagg tatcctgcca gcacatcaag
    22561 gcgcttttca ctgtagggcg tgagataaca acctgtaatg gcatctgcca gcgagggcaa
    22621 ttccggcgtg ttaatgtcct gcgggcattc gcgccacatt tttgccagtg ccggagcgtg
    22681 ccagccaccg cagacgacta acacgtcgcc attattttgc tgcaccgccc atgcaatcca
    22741 gcgagccata aaggcttcgc gctgacgatt gagtgcatcg ccggggaaat cgccccgcag
    22801 ttgggcaaaa tagtgcgcca gcgcagaggg taatgcagtt tgctggcttt catcttcgaa
    22861 caagtgatcc cacagggtat cgctgttatc catgcgggtg gcacgcagca gtaaggcctg
    22921 gctttcatct tgcgtatcag gcgaatcgtc ctcttcttca ctctgcgccc agcaaggcaa
    22981 atcgatgaag taagtttgtg cctgaatacg acgtgcggct tgtagcgcct gccactccgg
    23041 cgaaaattca gcaaatggcg tccaggcacc acgcccgggg gctgcaccgt cctgatactg
    23101 gcaataactg taaatagcta ccggaagctg gtgggaaaaa aaaagttcgt ctacccggtc
    23161 attaaaatca gccgggcctt caatcaacac gtatcgtggc cgctggcttt cgattaacga
    23221 tttcaccagc cgggcgcagg ccggactatg atgccggatg ccgacaatta acggctcgct
    23281 catgcctctt tcctcacggc aggcggtggc gggcttgata ataagcctgc cagtgagctt
    23341 ctttatgtgt ggcgacacgc tgttcgaagt aacggcgcag acgagcgcga tcttcttcgt
    23401 tatctttgac gatggttccg gcaatacagt ccaccaggtc ggcaggctcc cccgcgcgat
    23461 tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct tcagcggtgg
    23521 acataatcgc tgtcagtgta tccattgacg ttttcttttc gccgttggca cgcagatcgc
    23581 ggaaggtgcg taccagtaac tccagtactg cgtcaggcac tttatgcgga ataccgctat
    23641 gggccagcaa acgcgccgaa gcactggcga ccagttccag ctcctgggcg aaatccataa
    23701 tcggaaagac ggtttcaaaa tcgaagcgac gctttagcgc ggcactcatc tcattgacgc
    23761 cgcgatcgcg ggtattggca gtggcaataa tattaaagcc ttcccgcgca tagagctgac
    23821 tggcttcacc ggtgagttcc ggcaccgtca tcaccctgtc agaaagcata cctaacagac
    23881 agtcttgtac ttccagcggt gtacgcgtta tctcttcaaa acggacgatt ttgccgtcgc
    23941 gcattccctg gtaaagcggg gcagggacca gtgcttccgt tgacggacca tggttgataa
    24001 gcagggcgta gttccagccg tacttgattt gatcttcggt agtagatgcg ccgccctgga
    24061 tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa ttcagacagg agagattttg
    24121 cggtgccagg ttcccccacc aacatcaaac cgcgcccggt ggcaagagtg actaacatgc
    24181 gttcgacagc ggagggacta atcaccactt tacggctaat attttgcgcg ctatcgccaa
    24241 ggataaaagc acgggccgca ggcaaactta actgccaacc gggcgggcaa ggtgcgttat
    24301 cattttgttt taattttgcc agttcatcgg cgtataacac agcagcaggc ggacgctgca
    24361 gatgatt
//
LOCUS       DAPPNJ010000059        23926 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000059 DAPPNJ010000000
VERSION     DAPPNJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.059"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(11..175)
                     /locus_tag="RPM73_001909"
     CDS             complement(11..175)
                     /locus_tag="RPM73_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520276.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            284..778
                     /gene="napF"
                     /locus_tag="RPM73_001910"
     CDS             284..778
                     /gene="napF"
                     /locus_tag="RPM73_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDX6520277.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            768..1031
                     /gene="napD"
                     /locus_tag="RPM73_001911"
     CDS             768..1031
                     /gene="napD"
                     /locus_tag="RPM73_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDX6520278.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1028..3514
                     /gene="napA"
                     /locus_tag="RPM73_001912"
     CDS             1028..3514
                     /gene="napA"
                     /locus_tag="RPM73_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDX6520279.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3521..4216
                     /gene="napG"
                     /locus_tag="RPM73_001913"
     CDS             3521..4216
                     /gene="napG"
                     /locus_tag="RPM73_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDX6520280.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4203..5066
                     /gene="napH"
                     /locus_tag="RPM73_001914"
     CDS             4203..5066
                     /gene="napH"
                     /locus_tag="RPM73_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDX6520281.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5063..5512
                     /gene="napB"
                     /locus_tag="RPM73_001915"
     CDS             5063..5512
                     /gene="napB"
                     /locus_tag="RPM73_001915"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDX6520282.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5522..6124
                     /gene="napC"
                     /locus_tag="RPM73_001916"
     CDS             5522..6124
                     /gene="napC"
                     /locus_tag="RPM73_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDX6520283.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6143..6760
                     /gene="ccmA"
                     /locus_tag="RPM73_001917"
     CDS             6143..6760
                     /gene="ccmA"
                     /locus_tag="RPM73_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDX6520284.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNEGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6757..7419
                     /gene="ccmB"
                     /locus_tag="RPM73_001918"
     CDS             6757..7419
                     /gene="ccmB"
                     /locus_tag="RPM73_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDX6520285.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7461..8198
                     /gene="ccmC"
                     /locus_tag="RPM73_001919"
     CDS             7461..8198
                     /gene="ccmC"
                     /locus_tag="RPM73_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDX6520286.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8195..8404
                     /gene="ccmD"
                     /locus_tag="RPM73_001920"
     CDS             8195..8404
                     /gene="ccmD"
                     /locus_tag="RPM73_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDX6520287.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8401..8880
                     /gene="ccmE"
                     /locus_tag="RPM73_001921"
     CDS             8401..8880
                     /gene="ccmE"
                     /locus_tag="RPM73_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDX6520288.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8877..10820
                     /gene="ccmF"
                     /locus_tag="RPM73_001922"
     CDS             8877..10820
                     /gene="ccmF"
                     /locus_tag="RPM73_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDX6520289.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10817..11374
                     /gene="dsbE"
                     /locus_tag="RPM73_001923"
     CDS             10817..11374
                     /gene="dsbE"
                     /locus_tag="RPM73_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDX6520290.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11371..12423
                     /gene="ccmH"
                     /locus_tag="RPM73_001924"
     CDS             11371..12423
                     /gene="ccmH"
                     /locus_tag="RPM73_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDX6520291.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12458..13105)
                     /gene="narP"
                     /locus_tag="RPM73_001925"
     CDS             complement(12458..13105)
                     /gene="narP"
                     /locus_tag="RPM73_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDX6520292.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13425..16016
                     /gene="yejO"
                     /locus_tag="RPM73_001926"
                     /pseudo
     CDS             13425..16016
                     /gene="yejO"
                     /locus_tag="RPM73_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16120..16196)
                     /locus_tag="RPM73_001927"
     tRNA            complement(16120..16196)
                     /locus_tag="RPM73_001927"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16160..16162),aa:Pro,seq:ggg)
     gene            complement(16271..18031)
                     /gene="pbgA"
                     /locus_tag="RPM73_001928"
     CDS             complement(16271..18031)
                     /gene="pbgA"
                     /locus_tag="RPM73_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDX6520293.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(18051..18278)
                     /locus_tag="RPM73_001929"
     CDS             complement(18051..18278)
                     /locus_tag="RPM73_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDX6520294.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18460..19467
                     /gene="yejK"
                     /locus_tag="RPM73_001930"
     CDS             18460..19467
                     /gene="yejK"
                     /locus_tag="RPM73_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDX6520295.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19606..19890)
                     /gene="rplY"
                     /locus_tag="RPM73_001931"
     CDS             complement(19606..19890)
                     /gene="rplY"
                     /locus_tag="RPM73_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDX6520296.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(20015..21775)
                     /locus_tag="RPM73_001932"
     CDS             complement(20015..21775)
                     /locus_tag="RPM73_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX6520297.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21924..22619
                     /gene="rsuA"
                     /locus_tag="RPM73_001933"
     CDS             21924..22619
                     /gene="rsuA"
                     /locus_tag="RPM73_001933"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDX6520298.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22647..23837
                     /gene="bcr"
                     /locus_tag="RPM73_001934"
     CDS             22647..23837
                     /gene="bcr"
                     /locus_tag="RPM73_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDX6520299.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
BASE COUNT         5389 a         5900 c         6771 g         5866 t
ORIGIN      
        1 tcttttagtg ttaaattctg atgagggagc aaaccagcag aaatatctct ggtgaattaa
       61 tcgctatata aatatgttta taaccatttg aaatgtgagc aaaagcccgt ttttgccacg
      121 cttcccgcca ctcttttgat cctgctacag gttttacccc gatcggggta tgcatctttg
      181 acacatcctt taatatctta gcggctataa aaatggctta ttaattatgc ggtttatttg
      241 gtcgctctca attttcagag cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc
      301 ccgtcggggc atactcactg gtcgctggcg caaagccagt aacggtatcc gtccaccctg
      361 gtccggtgat gaatctcatt ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc
      421 ttgtgaaaac aacattctgc aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa
      481 taacgagtgc agcttctgtt acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc
      541 gcgccacacc agggcctggg atttgcagtt caccattggg gacgcgtgcc tggcgtatca
      601 gtcagttgaa tgccgccgct gtcaggatag ctgtgagcca atggcaatta tctttcgccc
      661 gacgctctcc ggaatctacc agccgcaact taatagccaa ctttgcaacg gatgtggagc
      721 atgtgcagcc agctgtccgg tatcagccat aactgcggag tatctccatg cacactaact
      781 ggcaagtttg cagcctggtc gtgcaggcca aaagcgaacg aatttcagac atcagcaccc
      841 aactgaacgc ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga
      901 ttgtggtggt ggaagcagaa gacagcgaaa cgctgatcca aaccattgag tcagtacgca
      961 acgtagaggg cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag caaggtgagg
     1021 aaacaccatg aaactcagtc gtcgtagctt tatgaaagct aacgccgttg cggccgctgc
     1081 ggcggctgcc ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc
     1141 cattaaatgg gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg
     1201 aacgcagcag gggcgtgtgg tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg
     1261 cctgaactgc attaagggct atttcctgcc caagatcatg tacggtaaag accgtttgac
     1321 gcagccgctg ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat
     1381 cacctgggat caggccttcg atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa
     1441 aggaccggaa tcgatcggta tgttcggttc tggtcagtgg actatctggg aaggttatgc
     1501 cgcgtccaag ctgttcaaag cgggcttccg ttcgaacaac atcgacccga acgcacgtca
     1561 ctgtatggcg tcggcagtag ttggctttat gcgtaccttt ggtatggatg agccgatggg
     1621 ctgctatgac gacatcgagc aggctgacgc gtttgtgctg tggggcgcta acatggcgga
     1681 gatgcacccg atcctctggt cacgcatcac taaccgtcgt ctctctaacc agaacgtcac
     1741 cgtggcggtg ctttctacct accagcatcg tagcttcgag ctggcggata acggcatcat
     1801 ctttacgccg caatctgacc tggtgatcct gaactacatc gccaactata tcattcaaaa
     1861 caatgcgata aatcaggact tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga
     1921 catcggctac ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga atccgggttc
     1981 tgacgcctcc gaaccgatga gctttgaaga ttacaaagcc ttcgttgctg agtatacgct
     2041 ggaaaaaacc gctgaaatga ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct
     2101 gtatgccgat ccgaacaaga aagtcatctc ctactggacg atgggcttca accagcatac
     2161 ccgtggtgtg tgggccaaca acctggtcta caacctgcac ctgctgaccg gcaaaatttc
     2221 ccagccgggt tgcggtccgt tctccctgac cgggcagcct tctgcgtgtg gtactgcgcg
     2281 tgaagtgggc acctttgctc accgtctgcc tgcggacatg gtggtgacta acgagaaaca
     2341 tcgtgatatc tgcgagaaga agtggaatat cccgggcggc accattccgg caaaaatcgg
     2401 tctgcacgcg gtggcacaag accgtgcgct gaaagacggc aagctgaatg tttactggac
     2461 catgtgtacc aacaacatgc aggccgggcc gaacatcaat gaagagcgta tgccgggctg
     2521 gcgcgatccg cgcaacttca tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc
     2581 cgccgacttg atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc
     2641 cgaacgccgt actcagttct ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga
     2701 tctctggcag ttagtccagt tctcccgccg cttcaaaact gaagaagtat ggccggaaga
     2761 gctgctggcg aagaaaccgg aactgcgtgg caaaacgctg tacgaagttc tgtatgccac
     2821 ccccgaagtg agcaaattcc cggtatccga actggcggaa gatcagctga acgatgaatc
     2881 ccgcgagctg ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg
     2941 cggtcacggt cacgatctcg cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg
     3001 gccggtggtg aacggtaaag aaacacagtg gcgttacagc gaaggtaacg acccgtacgt
     3061 gaaagcgggc gaaggctata agttctacgg taaaccggat ggcaaagcgg tgatcttcgc
     3121 actgccgttc gaaccggcgg cggaagcacc ggatgaagag tacgacctgt ggctctctac
     3181 cggacgcgtt ctggagcact ggcacaccgg cagtatgact cgccgtgtgc cggaactgca
     3241 ccgcgccttc ccggaagcgg tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct
     3301 gcgccgtggc gacaaagtga aagtggtttc tcgccgtggc gaagtgatct cgattgttga
     3361 aacgcgcggt cgtaaccgtc cgccacaggg cctggtgtac atgccgttct tcgacgccgc
     3421 acagctggtt aacaaactga cgctggatgc gaccgatccg ctctcgaaag agacggactt
     3481 caagaagtgc gcagtcaaac tggagaaggt gtaagccatt atgtcccggt cagcgaaacc
     3541 tcaaaatggt cgccgccgct ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc
     3601 cgtgggtgtg gcgctggggt tacaacagca aaccgcacgc gcatctggcg tgcggttgcg
     3661 cccgcccgga gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg
     3721 tgttcaggct tgcccttacg acaccttaaa actggcgacg ctggcctctg gtctgtcggc
     3781 gggcacgcca tattttgtcg cacgggatat tccttgcgaa atgtgtgagg acattccgtg
     3841 cgccaaagtg tgcccaagcg gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg
     3901 gatggggctg gcggtactgg tggaccagga aaactgtctc aactttcagg ggctgcgctg
     3961 cgatgtttgt tatcgcgaat gcccgaaaat tgatgaggcc atcaccctgg agctggagcg
     4021 caacacgcgt accggtaagc acgcccgctt tctgccgacg gttcacagcg acgcctgtac
     4081 cggttgcggt aagtgcgaaa aagtatgcgt gctggaacaa ccggcaatca aggtgttacc
     4141 gctgtcactg gcgaaagggg agttaggtca ccattaccgc ttcggttggc tggaggggaa
     4201 caatggcaaa tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc
     4261 gcagtcaccg ttggctggtg ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt
     4321 tgagcggtcc atggtttggt gtgtggatct tgcacggcaa ctacagcagt agcctgttat
     4381 tcgacaccgt tccgctgacc gatccgctga tgacgctgca aagtcttgcc agtggtcatc
     4441 tgcctgccac ggtggcgttg accggtgcgg tgattatcac cgtgctctac gccctggcgg
     4501 gaaagcgatt attttgcagc tgggtctgcc cgctgaaccc gattaccgac ttagcgaact
     4561 ggttacgcag gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac attcgctacg
     4621 tgctgttggt ggtcattctg ctgggatcgg ccttaactgg cacgctcatc tgggaatgga
     4681 tcaaccccgt atctctgatg gggcgcagcc tggtaatggg cttcggcagc ggcgcgctgc
     4741 ttattctcgc gctgttttta tttgatctac tggtcgttga acacggctgg tgcgggcaca
     4801 tttgtccggt tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg
     4861 ccaccgatcg gcagaaatgt aaccgctgta tggattgttt tcatgtttgc ccggaaccgc
     4921 atgtgctacg tgccccggtg ctggatgaac aaagcccggt gcaggtcacc agccgcgatt
     4981 gcatgacgtg cggtcgctgc gtggatgtct gttctgagga tgtatttaca ataactacac
     5041 gatggagttc gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg
     5101 gacggcgatg ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt
     5161 tagccagtcg ccggaagtat ccgggacaca ggaaggggcc attcggatgc caaaagagca
     5221 ggaacggatg ccgctgaact atgtgaatca gccgccaatg atcccgcata gcgttgaagg
     5281 ttatcaggta acgaccaata ccaaccgctg cttgcagtgc cacggtgtcg aaagttatcg
     5341 caccactggc gcgccgcgta tcagtcctac tcactttatg gacagcgacg gcaaagtggg
     5401 cgcggaagtg gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc aggccgatac
     5461 cgcgccaatc gtggggaata cctttacccc atcaaaaggt tacgggaaat aagaggttat
     5521 tatgggaaat tctgaccgta agcctggtct gattaagcgc ctgtggaaat ggtggcgtac
     5581 ccccagccgt ctggcgctgg ggacgctgct gttgatcggt tttgttggcg gcatcgtctt
     5641 ctggggtggc tttaacaccg ggatggaaaa agccaatacc gaagagttct gcattagctg
     5701 ccacgaaatg cgcaacacgg tgtatcagga atacatggat tccgtgcact acaacaaccg
     5761 tagcggcgtc cgtgcgacct gtccggattg tcacgttccg cacgagtttg tgccgaagat
     5821 gatacgcaag ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg ttattgacac
     5881 gccgcagaaa tttgaagctc atcgtctgac gatggcacag aatgagtggc ggcgcatgaa
     5941 ggacaataac tcgcaggagt gccgtaactg tcacaacttc gagtatatgg atacaaccgc
     6001 ccagaaatcg gttgccgcga agatgcatga ccaggcggtg aaagatgggc aaacctgtat
     6061 tgattgccat aaagggatag cgcacaagct gcccgatatg cgtgaagtcg agccaggttt
     6121 ttaacagggt tattgcgtgg gtatgcttga agccagagag ttactttgtg agcgggatga
     6181 acgaacctta tttagtggct tgtcatttac gctgaacgaa ggagagtggg tacaaatcac
     6241 cggtagcaac ggcgcgggga agacaacgct tctccgtttg ctgacggggt tgtctcgccc
     6301 tgacgcaggc gaggttctct ggcaagggca gcccttgcat caggtacgcg acagctacca
     6361 tcaaaacctg ttatggatag gccatcagcc ggggatcaaa acccggctga cggcgttaga
     6421 aaatctgcac ttttatcatc gcgatggcga taccgcacaa tgtctggaag ccctggcgca
     6481 ggccgggctt gccggattcg aagatattcc tgtaaatcag ctctcggccg ggcaacaacg
     6541 ccgcgtcgct ttagcgcgtc tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc
     6601 ttttaccgcg attgacgtta acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac
     6661 ggagcagggg gggattgtga ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa
     6721 aattcgccgc atttcactga cgcaaacgag ggccgcatga tgttctggcg cattttccgt
     6781 cttgagctgc gcgtagcgtt tcgccatagc gccgaaatcg ccaacccgct gtggttcttc
     6841 ctgattgtaa ttaccctttt tccgctcagt atcggtccgg agccgcaact gctggcgcgt
     6901 attgcaccgg gcattatctg ggttgctgcg ctgctttcat ccttgctggc gctggaacga
     6961 ctgttccgtg acgatttgca ggacggcagt cttgaacaat tgatgttgtt gccgttaccc
     7021 ttgcccgccg ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta
     7081 ctcatccttt cgccactggt agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg
     7141 atggcgctga cgctgctgct gggaacgcct acgcttggct ttctcggtgc accgggtgtg
     7201 gcgttgactg tgggacttaa gcgcggtggt gtgctgctca gcatactggt gttaccgctg
     7261 actatcccat tactcatctt tgccaccgcc gcgatggacg cggcttccat gcatttgccc
     7321 gttgacgggt atctggcaat tttaggcgcg ttgctggcag gcaccgcgac attaagtcct
     7381 tttgcgacgg cggcagcgtt acgaatcagc attcaataac gctggattac tgatttttgt
     7441 tcgagtctgg tatcgaaact atgtggaaaa cactgcatca actggcgatc ccaccacggc
     7501 tgtatcaaat ctgtggctgg tttataccgt ggctggcaat tgccagtgtg gtcgtgctta
     7561 ccgtcggctg gatctgggga ttcggctttg ctccggctga ttatcagcag ggaaatagct
     7621 accgcattat ctacctgcat gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa
     7681 tggcggtggc ggcttttatt ggccttgtct ggcagatgaa aatggccaac ctggcggtgg
     7741 cggcgatggc ccccattggt gccgtgttta cctttattgc cctggttacc ggctctgcat
     7801 ggggaaaacc gatgtggggc acctggtggg tatgggatgc acgtctgact tctgaactgg
     7861 tgctgctgtt tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc
     7921 tggcgggccg tgcggcaggt atcctggtgc tgattggcgt ggtgaatctg ccgattattc
     7981 attactccgt ggagtggtgg aacaccctgc atcagggatc aacgcggatg cagcaaagta
     8041 tcgatccggc gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg ctgctgtctg
     8101 ccacgctgac gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt
     8161 gggtgagtga actgatactg aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa
     8221 tgaatttttc gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt
     8281 tattccgctg gtggttttgg tcgtgcactc ggtgatgcag catcgcgcaa ttctgcgtgg
     8341 tgtggcgcaa cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc
     8401 atgaatattc gccgtaaaaa ccgcttgtgg atcgcctgtg ccgtgttggc agggctggcg
     8461 ctgactatcg gtctggtgct atatgcgctg cgctcgaata tcgatctctt ttatacgccg
     8521 ggggagattc tctacggcaa gcgtgagact cagcagatgc cggaagtcgg tcagcgtctg
     8581 cgcgttggcg ggatggtgat gccggggagt gtgcagcgcg atcccaattc gctaaaagta
     8641 actttcacca tttacgacgc tgaaggttca gtagatgtct cttacgaagg cattttgccg
     8701 gatctgttcc gtgaagggca gggcgttgtg gtgcagggcg aactggaaaa aggcaatcat
     8761 atcctcgcaa aagaagtgct ggcgaaacac gacgaaaact acacgccgcc agaagttgag
     8821 aaagcgatgg aagccaatca ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga
     8881 tgccggaaat tggtaacggg ctgctgtgtc tggcgctagg aattgcgctg ctgctgtccg
     8941 tgtatccgct atggggcgta gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt
     9001 ttgcctggct gctgtttatg tctgtggctg gcgcatttct ggtactggtc aatgccttcg
     9061 tggtcaacga cttcaccgtc acctatgttg ccagcaactc caatacccag cttccggtgt
     9121 ggtatcgcgt ggcggctacc tggggcgcgc atgaaggctc gctcctgctg tgggtgctgc
     9181 tgatgagcgg ctggaccttt gcggtagcga tttttagtca gcgtattccg ctggatattg
     9241 tggcccgcgt actggcgata atggggatgg tcagcgtcgg ctttttgctg ttcattctct
     9301 ttacctctaa cccgttctca cgcacgttgc cgaacttccc gattgaaggg cgcgatctta
     9361 acccgctgtt acaggatccg gggctgatct tccatccgcc tctgctctat atggggtacg
     9421 tggggttctc ggtggcgttt gcttttgcca ttgcttcttt gttgagcggg cgtctggaca
     9481 gcacttatgc gcgttttact cgtccgtgga cgctggcggc gtggatcttc ttgacgctcg
     9541 gcattgtgct cggttccgca tgggcctatt acgaactcgg ctggggcggc tggtggttct
     9601 gggatccggt agaaaacgcc tcgtttatgc cgtggctggt ggggactgcg ctgatgcact
     9661 cactggcggt cactgaacaa cgcgccagct tcaaagcgtg gacattactg ctggcaatca
     9721 gtgccttctc gttgtgtctg ctggggactt tcctggtgcg ttccggcgtg ctggtatcgg
     9781 tacacgcgtt tgcctctgat ccggcacgcg gtatgtttat cctcgccttt atggtactgg
     9841 tgattggtgg ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa
     9901 acaatgcgct gtggtcgcgg gaatctctgc tgttagcgaa caacgttttg ctggtcgccg
     9961 cgatgctggt ggtattactg gggacgctgc tgccgctggt gcacaagcaa ctgggactgg
    10021 gcagtatttc gattggcgaa ccgttcttca acaccatgtt tacctggctg atggtgccgt
    10081 ttgcgctgct gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa
    10141 tccgcaattt attgattatc gccttcatct caacgctggt gctgtcgctg ctgctgccgt
    10201 ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg gcctgctgga
    10261 ttgctgtgct ggcaattgcg gaagctgcgc tgcgtatttc acgtggcacg aaaaccacct
    10321 tcagttattg gggaatggtg gcggctcacc tggggctggc agtgacaatt gttggtattg
    10381 cctttagcca gaactatagc gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg
    10441 atattcatga atatcgcttc accttccgtg atgtcaaaga ggtgactggc ccgaactggc
    10501 gtggcggtgt ggcgactatc ggcgtaacgc gcgatggcaa gccggaaacg gtgctgtatg
    10561 cggaaaaacg ttattacaac actgccgggt cgatgatgac cgaagcggca attgacggcg
    10621 gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg
    10681 tgcgtcttta ctacaaacca tttgttcgct ggatttgggc gggcgggctg atgatggcgt
    10741 tgggcggact gctgtgtctg tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa
    10801 ctgcgccgga ggccgtatga agcgcaaagt attgttaatt ccgttgatta tcttcctggc
    10861 gattgccgcg gcgctgctgt ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa
    10921 tctggaatcg gcgctcattg gcaagcctgt gccgaagttt cgtctcgaat cactggacaa
    10981 tccggggcag ttttatcagg cggatgtgct gactcagggc aaaccagtgc tgcttaacgt
    11041 ctgggcgacc tggtgtccga cctgccgtgc ggaacatcaa tatctgaatc agctttcagc
    11101 gcagggcatt cgcgtggtcg gcatgaacta taaagacgat cgccagaagg caatcagctg
    11161 gctgaaagag ctgggcaatc cttacgcgct aagcctgttt gatggcgacg gcatgttagg
    11221 gctggatctc ggtgtctatg gcgcgccaga aaccttcctt attgacggca acggtatcat
    11281 tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct
    11341 gtgggagaaa tacagtaagg aggccgcaca atgaggtttt tattgggcgt gctgatgctg
    11401 atgatctccg gctcagcact ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag
    11461 gaacaacagt tccgtcagct cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc
    11521 attgccgatt ccaactcgat gattgccacc gacctgcgtc agaaagtgta tgaactgatg
    11581 caggaaggta aaagtaaaaa agagattgtc gattatatgg tggcgcgtta cggcaacttc
    11641 gtcacttacg atccgccgtt aacgccgctg accgtgctgc tgtgggtgct tccggtagtg
    11701 gctattggca ttggcggttg ggtcatatac gcccgctcgc ggcgtcgggt acgcgtagtg
    11761 ccggaagcgt ttcctgaaca aagcgtgcag gaaggtaagc gtgccggata tattgtttat
    11821 ctgccgggta ttgtggtggc gttaattgtg gctggcgtca gctactacca gactggcaat
    11881 tatcagcagg tgaaaatctg gcagcaggcc acggcacagg ctccggcgtt gctggacagg
    11941 gcgctggatc cgaaagccga tccgctcaac gaagaagaga tgtcgcgtct tgcgctgggg
    12001 atgcgtactc aactgcaaaa aaatccggga gatatagaag gctggattat gttgggccgc
    12061 gttggcatgg cgctgggtaa cgctagtatc gccaccgatg catacgctac tgcgtatcgc
    12121 ctcgatccga aaaacagcga tgccgcactg ggttatgctg aagcgttgac acgttcatct
    12181 gatcccaacg acaaccgcct cggtggtgaa ctgctgcgcc agttggtgag aagcgaccac
    12241 agcaatatcc gtgtgttaag catgtatgcg tttaatgcct ttgagcagca gcgatttggc
    12301 gaagccgttg ccgcgtggga gatgatgttg aaactcttac ctgccaacga tactcgccgt
    12361 gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa
    12421 taaaaagagg cccgatggtg atgccatcgg gctattttta ttgtgccccg cgttgttgca
    12481 ggaacagaat ggtggctgcc acgcgtgagc ggacattgag tttgcgcagc agattgcgaa
    12541 tatgtacttt tactgtctgc tctgaaatat tcaacaccga ggcaatctgt ttatttgaca
    12601 gcccctgtgc cagctcgtgc agaacatcca gctcgcgctc cgtcagcacg ctgaagggat
    12661 cttcttccgc gccaaacatt tcacgttcac gtaagtactg attgacgcgt tcgctaaaga
    12721 ctttgctgcc tttcgctcca gcacgaattg cttccagcaa tacttccggg tcgctgtctt
    12781 tcaacagata accgtctgcg cctgcgtcta tcagtgcaaa gacatcgctg gaggcatctg
    12841 atacggtcag gataataatt tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat
    12901 ccaggccact catacctttc atattgagat ccagcaagat cacgtcgata tccagtctat
    12961 tcgccagatc gatcgcgctc gcgccgtcgc ccgcttcggc gaccacttca aagccaggat
    13021 caagctccag taactgacga acaccgcgtc gcataagtgg atgatcatcc acaatcatca
    13081 cctgaaaagg cgttgcttca ggcatagtag tctcctgagg ttttattaga atcattatgt
    13141 ttgagcgtag cgttggtaac gctaccaggc gcgtgaatgt taccccattt tatgtatgga
    13201 catgtagtga aaactacaac cttgtcgttc gctttggcat cattttcatt ataaagacgg
    13261 aattttgcat cagcttctca tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc
    13321 atttctttaa acagttttta aaaactgact ccgggtatgg agctatgggt attttccgta
    13381 cccaatgctt ttaacagcaa ttaatttcat aggatgaaag ctcaattcat caatctggtt
    13441 ctgtttctct ttgtcgttcc gcaatatctg ttctggtggc tacagcgtta tattcaccca
    13501 tagcattggc atcaacagtt gagtatggtg agactgttga tggtgttgtc ctggaaaaag
    13561 atatccagct ggtttatggg accgccaata atacgaaaat caatcctggc ggagaacagc
    13621 atataaaaga atttggtgta agtagtaata ctgaaattaa cggagggtat cagtacattg
    13681 aaatgaatgg taccgcagaa tactcagtat taaatgacgg ttatcaaatt gttcaaatgg
    13741 gtggcgcggc aaaccagact accctcaata atggtgtgct acaggtttat ggcgcagcga
    13801 atgatcccac gattaaaggc gggcgcttaa tcgttgaaaa agatgggatt accgtccttg
    13861 ccgctatcga aaagggagga ttactggagg ttaaagaggg gggattagcg attgcggtag
    13921 atcagaaagc aggcggtgct attaaagcaa gcacgcgggt catggaggta ttcggaacaa
    13981 accgtctcgg tcagttcgaa atcaagaatg gtattgctaa caatatgctg ttggaaaacg
    14041 gcggaagttt gcgagttgaa gaaaatgact tcgcttataa tactactgta gatagtggcg
    14101 gcttactgga ggttatggat ggcgggactg caactggcgt tgataaaaaa gcaggcggaa
    14161 aattaattgt ctcaacgaat gcgctggaag tgagtggtac aaacagtaaa ggccaattta
    14221 gtataaaaga tggtgtgtca aaaaattatg aactggatga tggttccggg ctcattgtta
    14281 tggaggacac gcaggccatt gatactatcc ttgatgagca tgccactatg caatcgctgg
    14341 gaaaggatac tggtacgaaa gtgcaggcaa atgcggtata tgatctcggt cgatcagatc
    14401 agaatggaag tatcacgtat tcctcaaaag ccatctctga aaatatggtt atcaacaatg
    14461 gccgcgctaa cgtctgggct ggcacaatgg ttaacgtttc agtcagaggg aatgatggca
    14521 ttcttgaggt catgaagccg caaataaatt atgcacccgc aatgttggtg ggtaaggtag
    14581 tggtttctga gggcgcttct tttagaacgc atggtgccgt ggataccagc aaagcggacg
    14641 tttcgctcga aaatagcgta tggaccatca ttgccgatat cactacgacg aaccaaaaca
    14701 ccctcctcaa cttagccaac cttgcgatgt ctgacgcaaa tgtgattatg atggatgagc
    14761 cagtgactcg ttcatcagtg acggcaagtg cggaaaattt cattacgttg accaccaata
    14821 ccctgtcggg aaacggcaat ttttatatgc gtaccgatat ggctaatcat cagagcgatc
    14881 agctcaacgt caccggtcag gcaacaggtg atttcaaaat attcgtgacg gacaccggtg
    14941 ccagcccggc agcaggagat agccttacac tggtaacaac gggcggcggt gatgctgcat
    15001 ttacgttggg caatgccgga ggcgttgttg atatcggtac gtatgaatat accttgctgg
    15061 ataatggcaa ccatagctgg agtttggcag agaatcgcgc gcaaattacc ccttcaacca
    15121 ctgatgtgct gaatatggcg gcagcacaac cgctggtatt tgatgcagaa ctggacaccg
    15181 tgcgtgagcg tcttggtagc gtaaaaggcg ttaattacga tacggcgatg tggagttcgg
    15241 caattaacac ccgcaacaac gtgaccactg atgcgggcgc tggctttgag caaacattga
    15301 ccgggctgac gctcggtatc gatagccgtt tctcccgtga agaaaccagc acaattcgcg
    15361 gcttgttctt tggttactct cattctgata ttggttttga tcgcggcggc aaaggcaatg
    15421 tcgatagcta taccctgggg gcttatgccg gttgggagca tcagaacggt gcctatgttg
    15481 atggagtggt gaaagttgac cgttttgcca acaccatcca ttgcaagatg agtaatgggg
    15541 caacagcgtt tggcgattac aatagtaacg gcgcgggtgc tcatgtcgag agcgggttcc
    15601 gttgggttga cggattgtgg agtgttagac cctatctggc ctttaccggc tttaccacag
    15661 atggtcagga ctacacgtta tcaaacggca tgcgcgcgga tgtgggaaat acccggatat
    15721 tacgcgctga agcgggaacg gcggtaagct atcacatgga cctgcaaaac ggtacgacgc
    15781 tggaaccctg actgaaagcc gccgtgcgtc aggaatacgc cgattctaac caggtgaaag
    15841 ttaatgacga tggcaaattt aataatgatg tggctggaac ccgtggcgtt tatcaggctg
    15901 ggataaggtc atcgtttacc ccgacgttaa gcggtcattt gtcagtcagc tatggcaatg
    15961 gcgcaggggt agaatcgccg tggaatactc aggcgggtgt ggtctggacg ttctgataac
    16021 aaaaaataaa caggctgtga tgtgtcacgg tctgtttatc gaattaattg cagatataaa
    16081 aaaaaccaac cgtaaaggtt ggttttttct tgggattttt ggtcggcacg agaggatttg
    16141 aacctccgac ccccgacacc ccatgacggt gcgctaccag gctgcgctac gtgccgactc
    16201 gtggctgcta atactaccgt tttccacacc gattgcaagt aagatatttt gctaactgat
    16261 ttataattaa tcagttagcg ataaaacgct tctcgtctgt cagcacttgc aataacaaac
    16321 ttaactgtgg tttttcatct ttcactcttt caccacgtaa gttgtaagtg cggtatttac
    16381 cgttattgtt cagcaccagc gtctttttcg gggtggtaat tgccagcgta tcgttatccg
    16441 ctgcagtaac ccagtaatga cggcgttgag ggttgaacaa atcctgacct tgcgaatatt
    16501 cgctggcagg tgtgctgaca tgtagcaggc gttgcatcag cgtcgtcatc agatcggtat
    16561 gatcagtcag agcattaata cgctgcgccg gcgtgcctgg ccagtgaatc actaatggca
    16621 cctgcagatg accgtgggac cagtcaaagg tttcttcgtc ttcgctcagc ggaatacccc
    16681 gaccggcagt gataatcacc accgtattgt ccagtttgcc agaatcacgc agtgcattga
    16741 gcacgcggtt gatctggtca tcgacattgc ctgccgcccg gctatatttc cgtgcaaatg
    16801 cctgctgatt gctgtcgtca atgttagtgc cattgaaaga gacccacgag aaccagcggt
    16861 tatcttcttg tgcgtagcgg cccagccagt tgatccactg cgtggcggtc tgctcgtcgg
    16921 attgggtgcg tacgctcggc atcgagaaat ctgacaacaa tgcctggcga tacagcgggc
    16981 tggtaaagcc atctgatgag aataatccca gctgatagcc ttgctgatta agcgcagtaa
    17041 ttaacgccgc aggggtacgg gtcgacagaa tgccgtccat atagctcggc gagatgccat
    17101 agaacaggcc aaagatgccg ttgtctgtag tgttgccgga gctcatatgg cgcgtgaacg
    17161 aaatattttg ctcagcaaaa cctgccagcg caggcatctg cttctcgaag cgtgagtagt
    17221 tcaggccatc gacagtaatc aacagcacat tctgcccggt gcccatatcg cgatagcgca
    17281 gttcgcttaa cggatactga acggaaacgg cgtctggatt accttgctca ataagacggc
    17341 gttgatactc ctgcgcatca agcagaccat gcttctcaag aaaacgtcgc gccgtcatcg
    17401 ggtacgaaag cggcaggtta gcgcgctgca tggtgatcgg gcgatagaag ttggcatcgg
    17461 cccagatata caccacatgc gaggcgataa aggcgataaa taagaatgcg gccagcgggc
    17521 gcgcgaagcg ccgacgacgc gtcaggctgc gcagcttttg ccagctccac gtcgcaaaca
    17581 ccagttcaag caataaaata accggcacgc tgatgaacat cagctgccag tcgcgcgcca
    17641 tctcattttc gtctgggttg ataaccagtt gccagacgat gggattaaga tggagatgga
    17701 aacgagtaaa gacttcgcta tcgatcagta atagcgtcat tcccgccgtt gccagaatga
    17761 cggacaaaaa cctcatcagc ctctgggagc cgacgataaa ggtcagcggg aagaggatca
    17821 gcaagtaggt ggcgaacacc aggaagctga aatggccgat aatgcttacg taggaataaa
    17881 tgcgaccagc aagcgttgtc ggccagtcgg cgataaacag gtaacggctg ccaatgacga
    17941 gcgaaagcag aatattgaac agtgcaaacc agtgccccca actgaccatc tgggagactt
    18001 tttcacggta gcgctgacga tgagttacca taacctgtta tctgtttccc ttagtgcgct
    18061 ttgtcttcgt tgatagagga ctgtaaggcg cgggcaaaag agttggcaat tgcctggcgt
    18121 tgggccgggg caatgctggt attgataagg ttagtgacca tgttgcctaa caccatcagg
    18181 gaaaggtcgg tcggagcctt atgtttttcc agtacgttga gcagctctgc gagcaattgt
    18241 tcaacctgtt catcactgta gcgggaaatt tgtggcataa atcgaaatca gttagttgat
    18301 gaaagggcaa catattaccg tagcaacggt tttttttctg cttttttatg cgttaaatca
    18361 ttctcgttac gctgccttct gtaaaatgac cgccatcttt tattgcgtcg cttaaccggc
    18421 ggtggttgaa taccgcccgg tcttaaagga gagtttatca tgagtctgga tatcaaccag
    18481 attgctctgc accagcttat caagcgtgat gagcagaacc ttgagctggt gttgcgcgat
    18541 tcattgctgg aaccgacaga aaccgtcgtt gagatggtgg cagaactgca tcgggtctat
    18601 agcgccaaaa ataaagctta cggcttgttt agcgaagaga gtgaactggc gcagacgctg
    18661 cgcttacagc gtcagggcga agaggacttt ctggcattta gccgcgcggc aaccgggcgc
    18721 ctgcgtgacg aattagcgaa atatcctttt gctgacggtg gattcgtgct gttttgccac
    18781 taccgttatc tggcggtgga gtatctgctg gtggcagttc tgagcaacct gagcagtatg
    18841 cgcgttaacg aaaatctcga catcaatcca acccattatc ttgatatcaa tcatgcggat
    18901 attgttgcgc gtatcgattt aaccgaatgg gaaaccaatc cagagtccac ccgctatctc
    18961 actttcctga aagggcgagt agggcgcaaa gtggcggatt tctttatgga tttcctcggg
    19021 gccagcgaag ggttaaacgc gaaagcgcaa aaccgtgggc tgttgcaagc ggttgatgat
    19081 ttcactgccg aagcacaact ggataaagca gagcgacaga acgtgcgcca gcaggtttat
    19141 agctactgta atgagcagct gcaggcaggc gaagagatcg aactggagtc gctgtcgaaa
    19201 gagctggcag gcgtcagcga agtgagtttc acggagtttg cggctgaaaa aggctacgaa
    19261 ctggaagaga gcttcccggc agaccgtagc acactgcgtc agttgacgaa gtttgccggt
    19321 agcggcggtg gcttgactat taactttgat gcgatgctgt taggtgaacg catcttctgg
    19381 gatccggcaa ccgataccct gaccattaaa ggcacgccgc cgaatttgcg tgaccaattg
    19441 cagcgccgga cgtctggcgg caattaatcg gttttgtgtt ctgaatgcca gatgcggcgt
    19501 gaacgcctta tctggcctgc aaaaaatagc aataccgtaa tcaggcataa tctgcagata
    19561 caaaaaaccc cgccggagcg gggtttttta caacttattc agcaattaag cgcgaacgaa
    19621 gtcgatgtgc tgcagcttcg gtttgtacgg gtgacgctgt acgtcctgag ctttaacttt
    19681 gatttcttta ccgtcaacaa cgatggtcag aacttcgctg tagaattcag ctttagcttg
    19741 catgttcatg actttgtcgt gatccagctc gatagccagc ggcgcttctt tgccaccgta
    19801 gatgattgcc gggaacttgt tagcggcacg caggcggcgg ctcgcaccct taccctgctc
    19861 tttacgtact tctgcgttga tagtaaacat ttctttctct cttaaattaa tcctgctaca
    19921 ggcgacccag caacaggtaa gtgatctgct ttgcgtatgc aaaagcgggc gagattctat
    19981 actcaatcgc tttatacatc aatcaaaact accattaacc gcgtaattcg tgcgcccgac
    20041 gaaaacgacc ttcgtaatcg aacacttttt cacgcacttg ccagtactga cctttcatgc
    20101 gggcaacgac aaaatccggg tgtcgcaata aggcttgctg ggcgaggata tcggcggcgg
    20161 tgatccagcg cagagggatg cccggtgtgc gcgtatgcgg gcggataaaa agctgctcga
    20221 aggcagttcg ctgggcgggc gtttgcagac ggaaacgctc actcacatcc gcgccgtctt
    20281 catcgtaata ggtgattttc aaccattcgc ctttctcgtc gtgctcatgc tgcaaagaca
    20341 tgccgctaca gcgtaatacc agcgcatctt tcagtcgtag cgccgctttt aacatatcat
    20401 ccggatcaac cagtaccgtg tcacattcgc ggcagcggcg agcggcaata tcattttctg
    20461 cgttacattg cgggcaattt ttaaaacgga aacggaagtc acactgttcg cgatgaccgt
    20521 cgtcatcttc aaaccatccc tgacagcggc gaccaaagtg ttcaatcaat gtcccgtcgg
    20581 cggtcgtttt cccccaaaag gtgttggcaa aaccgcaggc agggcagaaa acctgtaccg
    20641 gaacgttgtc acttttgcct ttcggtgtac caacttccgg cgcgtagaga tcgtgaggat
    20701 tacccgcata atcaagaatt aaacaatcag tcttgcccgg agcgagacgc agaccgcgcc
    20761 cgacaatttg ttggtaaaga ctcactgatt cggtagggcg cagaatggcg ataagatcga
    20821 cgtgcggggc gtcaaatccg gtggtcagta ctgcgacgtt gaccagatag cgaaaacgct
    20881 gggctttaaa atcttcaatt aacacatcgc gctcagcgcc gggggtgtcg ccagtaatca
    20941 gtgcagcatc ttcggcaggc agtaatccca caatctcttt tgcgtgttca acggtggcgg
    21001 cgaaaatcat tacccctttg cgcttttcgg cgaactccat gatctggctg atgatatgtg
    21061 gcgtaatgcg ttgctgtttt ttcagctcac ggttgagatc agcttcgctg aacagcccgt
    21121 tactctgtgc ctgcaagcgg ctgaaatcgt attgcactac tggcatatcc agtcgttctg
    21181 gcggcgtcag atagccgtgt ttaatcatat aacgcagcgg cagctcataa atgcagtcac
    21241 ggaaaagggc tttctcatcg ccgcgtacca tgccgtgata atggaactgg tagatccagc
    21301 ctttgcccaa tcgaaaaggc gtggcagtca gccccagcag gcgtaagtgg ggattcactt
    21361 tagtcaggtg agtgaggatt tgctgatact ggctctcttc atcgtcacca atacggtggc
    21421 attcatcgac aatcaacagc gaaaattcac cctgaaaggc atcaagatta cgagcgaccg
    21481 actgcacgct gccaaatacc actttaccgt ggctctcttt gcgctttaac ccagcggcaa
    21541 aaatatcggc ttccagcccc agcgcctgat actttgcatg gttttgcgcc accagttctt
    21601 tgacgtgtgc cagcaccaga acacgaccac gcgccagccg tgccagttcg gcaatcacca
    21661 ggcttttacc tgcgccggtg ggcagcacga taacggcagg ggttttatga cgacgaaaat
    21721 ggttgagcgt ggcatccacg gcttcttgct gatatgggcg gagtgtaaaa atcatggtct
    21781 cgcgtaggta gattattctg ctaatagtat gccatggatc tttttccctt gagggatgtt
    21841 attagaccgc tatactgggc agacaacagt tcagattttc gggtcaatgc ccgacctccg
    21901 tttttacagg caaaatcaca cacatgcgac ttgataaatt tatcgcacag caactcggcg
    21961 ttagccgtgc tattgccggg cgtgaaatcc gcggcaatcg tgtcaccgtc gatggcgaaa
    22021 ttgttcgtaa tgcagcgttc aaactgcttc ctgaacatga tgtcgcttac gatggcaacc
    22081 cgctggcgca gcaacatggt ccacgttact tcatgctcaa taagcctcag ggctatgttt
    22141 gctccacgga cgaccctgat cacccaacgg tgctctattt tctggatgaa ccggtagcct
    22201 ggaaactgca cgccgcagga cgtctggata tcgataccac cggtctggtg ctgatgactg
    22261 atgatggtca gtggtcgcac cgcattactt ctccgcgcca tcattgcgag aagacctatc
    22321 tggtgacgct ggaatcaccc gtagctgacg atacggcaga gcaatttgcc aaaggcgtac
    22381 agctgcataa cgaaaaagat ctcactaagc cagcggtgct ggaagtgatt acccctacgc
    22441 aggtccgtct gaccatcagc gaagggcgtt atcatcaggt gaaacgcatg ttcgccgctg
    22501 tgggtaacca tgtggttgag ctgcatcgtg aacgtattgg cggtattacg ctggatgctg
    22561 atttagcccc cggtgaatat cgtccgttaa ctgaagaaga aattgccagc gtcgtctaac
    22621 ttttccatga ctttcaggag cccgttgtga ccacccgaca gcattcgtcg tttgctattg
    22681 tttttatcct tggcctgctg gccatgttga tgccgctgtc gattgatatg tatctgcccg
    22741 cgctaccggt aatttcagcg cagtttggcg tacctgcggg cagtacgcag atgaccctca
    22801 gtacttatat tctgggcttt gcgttggggc agttaatcta cgggccgatg gcagacagct
    22861 ttgggcgtaa gccggtggtt cttggcggta cgctggtgtt tgccgccgcc gcggtggcgt
    22921 gtgcgttggc acaaaccatc gatcagctga ttgtgatgcg tttcttccac gggctggcgg
    22981 cggctgcggc cagcgtggtt atcaacgctt tgatgcgtga tatctacccg aaagaagagt
    23041 tctcgcggat gatgtcgttt gtcatgctgg tgacgaccat tgcaccgctg atggcaccga
    23101 tagttggcgg ctgggtgctg gtgtggctga gctggcatta catcttctgg atcctcgccg
    23161 tggcggcgat tctggcttcg gcaatgatct tctttctgat caaagaaacc ttaccaccgg
    23221 agcgtcgcca gccttttcac attcgtacca ctattggtaa ctttgcggcg ctgttccgtc
    23281 ataagcgtgt cctgagttat atgcttgcca gtggtttcag ctttgccggg atgttctcgt
    23341 tcttaagcgc cggaccgttt gtctacattg aaattaacca cgtcgcgccg gaaaactttg
    23401 gttattactt tgcactaaac attgttttcc tgttcgtgat gaccattttt aacagccgct
    23461 tcgtccgccg tattggcgcg ttaaatatgt tccgctcggg gttgtggata cagtttatta
    23521 tggcggcgtg gatggtcatc agtgcgctgc tggggctggg attctggtcg ctggtggttg
    23581 gcgttgcggc gtttgtgggc tgcgtgtcga tggtgtcatc caatgcgatg gcggtcattc
    23641 ttgatgagtt tccccatatg gcgggaacgg catcttcgct ggcaggaacc ttccgttttg
    23701 gcataggggc aattgttggc gcattgcttt ctcttgcgac ctttaactct gcatggccga
    23761 tgatttggtc aattgcattc tgcgcaagca gctccattct cttctgtctg tacgccagtc
    23821 gaccgaaaaa acggtgatct attgcacaac gaggaagcta aaaggcttcc tttgttgatg
    23881 catgtcaacc acaaatctat cattcccccg atatatgttt atttta
//
LOCUS       DAPPNJ010000060        23800 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000060 DAPPNJ010000000
VERSION     DAPPNJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.060"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            31..576
                     /locus_tag="RPM73_001935"
     CDS             31..576
                     /locus_tag="RPM73_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDX6520300.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            551..2476
                     /locus_tag="RPM73_001936"
     CDS             551..2476
                     /locus_tag="RPM73_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDX6520301.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2473..2679
                     /locus_tag="RPM73_001937"
     CDS             2473..2679
                     /locus_tag="RPM73_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDX6520302.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2676..4277
                     /locus_tag="RPM73_001938"
     CDS             2676..4277
                     /locus_tag="RPM73_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDX6520303.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4258..5577
                     /locus_tag="RPM73_001939"
     CDS             4258..5577
                     /locus_tag="RPM73_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDX6520304.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQACVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5587..5919
                     /locus_tag="RPM73_001940"
     CDS             5587..5919
                     /locus_tag="RPM73_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDX6520305.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5975..7000
                     /locus_tag="RPM73_001941"
     CDS             5975..7000
                     /locus_tag="RPM73_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDX6520306.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIMFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7042..7437
                     /locus_tag="RPM73_001942"
     CDS             7042..7437
                     /locus_tag="RPM73_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDX6520307.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            7449..7802
                     /locus_tag="RPM73_001943"
     CDS             7449..7802
                     /locus_tag="RPM73_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDX6520308.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7814..8392
                     /locus_tag="RPM73_001944"
     CDS             7814..8392
                     /locus_tag="RPM73_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520309.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKSPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8389..8784
                     /locus_tag="RPM73_001945"
     CDS             8389..8784
                     /locus_tag="RPM73_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520310.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8792..9532
                     /locus_tag="RPM73_001946"
     CDS             8792..9532
                     /locus_tag="RPM73_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520311.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVRRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            9548..9970
                     /locus_tag="RPM73_001947"
     CDS             9548..9970
                     /locus_tag="RPM73_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDX6520312.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            9952..10386
                     /locus_tag="RPM73_001948"
     CDS             9952..10386
                     /locus_tag="RPM73_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDX6520313.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDTHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10379..12958
                     /locus_tag="RPM73_001949"
     CDS             10379..12958
                     /locus_tag="RPM73_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX6520314.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQGLAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRILALSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIEAKNRGLTDRQAAREATEQRLKEQYGDNSLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSG
                     GGR"
     gene            12955..13284
                     /locus_tag="RPM73_001950"
     CDS             12955..13284
                     /locus_tag="RPM73_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520315.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTFSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13284..13982
                     /locus_tag="RPM73_001951"
     CDS             13284..13982
                     /locus_tag="RPM73_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDX6520316.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSGADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13988..14731
                     /locus_tag="RPM73_001952"
     CDS             13988..14731
                     /locus_tag="RPM73_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX6520317.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            14668..15300
                     /locus_tag="RPM73_001953"
     CDS             14668..15300
                     /locus_tag="RPM73_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDX6520318.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15361..18759
                     /locus_tag="RPM73_001954"
     CDS             15361..18759
                     /locus_tag="RPM73_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDX6520319.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALF
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCM
                     WDMLTHPRYGMRKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKPPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            18826..19425
                     /locus_tag="RPM73_001955"
     CDS             18826..19425
                     /locus_tag="RPM73_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDX6520320.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19490..22561
                     /locus_tag="RPM73_001956"
     CDS             19490..22561
                     /locus_tag="RPM73_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDX6520321.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSATSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSASSSASS
                     ASASKDEATRQASAAKGSATTASMKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLSSKGRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSH
                     RDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAA
                     SAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22561..23136
                     /locus_tag="RPM73_001957"
     CDS             22561..23136
                     /locus_tag="RPM73_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDX6520322.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTEQDIEWPALP"
     gene            complement(23234..>23800)
                     /locus_tag="RPM73_001958"
     CDS             complement(23234..>23800)
                     /locus_tag="RPM73_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX6520323.1"
                     /translation="RISTLDQTTENQRREIESAGFKIKPQQIIEEHISGSAATSERPG
                     FNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGGIDLTSPTG
                     KMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVVFERIKSGV
                     SISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         5599 a         6030 c         7296 g         4875 t
ORIGIN      
        1 ctttctctgt ttttggccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac
       61 attttcggtg cgagtatccg taccattcag aactggcagg aacagggaat gcccgttctg
      121 cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg ccgccgttat aagatggtat
      181 gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgcgg
      241 caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg
      301 cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga agtggtggaa
      361 accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac
      421 gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc
      481 ctgaaacggg atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg
      541 gggttgctga gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc
      601 cgggcttcgc tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgctaa
      661 ttactatctc ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca
      721 gcgggccatc atgaatgcga tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc
      781 tgcccgtgtc ggttattcca aaatgctgct gggtgtttat gcctacttca tagagcataa
      841 gcagcgcaac acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac
      901 ccacgttgag ccgactattc gtgatattcc gtcgctgctg gcgctggctc cgtggtatgg
      961 caaaaagcac cgggataaca cgctcaccat gaagcgtttc actaatgggc gtggcttctg
     1021 gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta
     1081 tgatgaactt gctgcttttg atgatgatat tgaacaggaa ggctctccga cgttcctggg
     1141 tgacaagcgt attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt
     1201 gagaggcacc tgtcagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca
     1261 tgttgcctgc ccgcactgcg gggaggagca gtaccttaaa tttggcgata aagagacgcc
     1321 gtttggcctc aaatggacgc cggatgatcc ctccagcgtg ttttatctct gcgaacataa
     1381 tgcctgcgtc atccgccagc aggagctgga cttcactgat gcccgttata tctgcgaaaa
     1441 gaccgggatc tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga
     1501 accgcctgac agcgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt
     1561 gcagattgtc aaagactgga tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt
     1621 gaacaccacg ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt
     1681 gatggcagag cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac
     1741 tgccggtatc gactcccagc tggaccgcta cgaaatgcgc gtatggggat gggggccggg
     1801 tgaggaaagc tggctgattg accggcagat tattatgggt cgccacgacg atgaacagac
     1861 gctgctgcgt gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat
     1921 gtcggtatcc cgtatctgct gggatactgg cgggattgac ccgaccattg tgtatgaacg
     1981 ctcgaaaaaa catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa
     2041 gccggtggcc agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg
     2101 tacggatacc gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga
     2161 accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc
     2221 gcagcagctg actgctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact
     2281 gtgggacagc aaaaagcgac gcaatgaggc actcgactgc ttcgtttatg cgctggcggc
     2341 gctgcgcatc agtatttccc gctggcagct ggatctcagt gcgctgctgg cgagcctgca
     2401 ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc
     2461 cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac
     2521 ctgatgacag gtaaacgggt ggcaacagta cagaaagacg gacggcgagt ggagtttacg
     2581 gccacttccg tgtctgacct gaaaaaatat attgcagagc tggaagtgca gaccggcatg
     2641 acacagcgac gcaggggacc tgcaggattt tatgtatgaa aacgcccacc attcccaccc
     2701 ttctggggcc ggacggcatg acatcgctgc gtgaatatgc cggttatcat ggcggtggca
     2761 gcggatttgg tgggcagttg cgggcgtgga atccaccgag tgaaagtgtg gatgcagccc
     2821 tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtgcgc aataacggct
     2881 atgccgccaa cgccatccag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     2941 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     3001 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     3061 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     3121 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggctgttc cggacacagt
     3181 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     3241 gccgtgccgg tgtgcagatt aatgacagcg gcgcggcgct gggatattac gtcagcgagg
     3301 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     3361 ggcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa
     3421 atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc
     3481 tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat tgagagtgag ctggatacgc
     3541 agtcagcgat ggattttatt ctgggcgcga acagtcagga gcagcgggaa aggctgactg
     3601 gctggattgg tgaaattgcc gcgtattacg ccgcagcacc ggtccggctg ggaggcgcaa
     3661 aagtgccgca cctgatgccg ggggactcac tgaacttgca gacggctcag gacacggata
     3721 acggctactc cgtgtttgag cagtcactgt tgcggtatat tgctgccggg ctgggtgtct
     3781 cgtatgagca gctttcccgg aattacgccc agatgagcta ctccacggca cgggccagtg
     3841 cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga
     3901 gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt
     3961 caaaagcgcg cttcagtttt caggaagccc gcagtgcctg ggggaactgc gactggatag
     4021 gctccggtcg tatggccatc gatggtctga aagaagttca ggaagcggtg atgctgatag
     4081 aagccggact gagcacctac gagaaagagt gcgcgaaacg cggtgacgac tatcaggaaa
     4141 tttttgccca gcaggtccgt gaaacgatgg agcgccgcgc agccggtctt aaaccgcccg
     4201 cctgggcggc tgcggcattt gaatccggac tgcgacaatc aacagaggag gagaagagtg
     4261 acagcagagc tgcgtaatct cccgcatatt gccagtatgg cctttaatga gccgctgatg
     4321 cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg caggccagct tgggatcagc
     4381 cgcctgacgg atgcggtgtc cggcgacagc ctgactgccg gagaggcacc cgcggcgctg
     4441 gcgttatccg ttgatgatga cggaccacga caggcccgca gttatcaggt catgaacggc
     4501 atcgccgtgc tgccggtgtc cggcacgctg gtcagccgga cgcgggcgct gcagccgtat
     4561 tcggggatga ccggttacaa cggcattatc gcccgtctgc aacaggctgc cagcgatccg
     4621 atggtggacg gcattctgct ggatatggac acaccgggcg ggatggtggc gggagcattt
     4681 gactgtgctg acatcatcgc ccgtgtgcga gacataaaac cggtatgggc gctggccaac
     4741 gacatgaact gcagtgcagg tcagctgctt gccagcgccg cctcccggcg tctggtcacg
     4801 cagaccgccc ggacaggctc catcggcgtc atgatggctc acagtaatta cggtgctgcg
     4861 ctggagaaac agggcgtgga aatcacgctg atttacagcg gcagccataa ggtggatggc
     4921 aacccctaca gccatctacc ggatgatgtc cgggaaacat tgcagtcccg gatggatgca
     4981 acccgccgga tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg
     5041 ctggataccg aggctgcagt gtacagcggt caggaggcca ttgatgccgg actggctgat
     5101 gaacttgtca acagcaccga tgcgatcacc gttatgcgtg atgcactgga tgcacgtaaa
     5161 tcccgtctct caggagggcg aatgaccaaa gagactcaat caacaactgt ttcagccact
     5221 gcttcgcagg ctgacgttac tggcgtggtg caagcgacgg agggcgagaa cgccagcgct
     5281 gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagtcgcatt
     5341 atggggatcc tcaactgtga ggaggctcac ggacgcgaag aacaggcatg cgtgctggcc
     5401 gaaacccccg gtatgaccgt ggaaacggcc cgccgtattc tggccgcagc accacagagt
     5461 gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg
     5521 gctgcaggta acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg
     5581 atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa cagtgacccg
     5641 gctcataccg caaccgcgcc cggcgggttg agtgcgaaag cgcctgcaat gaccccgctg
     5701 atgctggaca cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc
     5761 gttggcattc ttgcagttgc tgctgaccag accagcacca cactgacgtt ctacaagtcc
     5821 ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa
     5881 cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac ctgacccttc atcactaaag
     5941 gccgcctgtg cggctttttt tacgggattt ttttatgtcg atgtacacaa ccgcccagct
     6001 gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc tctttttccg
     6061 tgagagctat cccttcacca cggagaaagt ctatctctca caaattcctg gactggtaaa
     6121 catggcgctg tacgtttcgc caattgtttc cggtgaggtt atccgttccc gtggcggctc
     6181 cacctctgaa tttacgccgg gttatgtcaa gccgaagcat gaggtgaatc cgcagatgac
     6241 cctgcgtcgc ctgccggatg aagatccgca gaatctggcg gacccggctt accgccgccg
     6301 tcgcatcatc ctgcagaaca tgcgtgacga agagctggcc attgctcagg tcgaagagat
     6361 gcaggcagtt tctgccgtgc tcaagggcaa atacaccatg accggtgaag ccttcgatcc
     6421 ggttgaggtg gatatgggcc gcagtgcggc gaacaacatc acgcagtccg gcggcacgga
     6481 gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag cctacgcgct
     6541 gaacgccagc ggcgtggtga atatcatcat gtttgatccg aaaggctggg cgctgttccg
     6601 ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc ggctctaatt ccgagctgga
     6661 gacagcggta aaagacctgg gcgaagcggt gtcctataag gggatgtatg gcgatacggc
     6721 gatcgtcgtg tattccggac agtacgtgga aaacgacgtc aaaaagaact tcctgccgga
     6781 caacacgatg gtgctgggga acactcaggc acgcggtctg cgcacctatg gctgcattca
     6841 ggatgcggac gcacagcgcg aaggtattaa cgcctctgcc cgctacccga aaaactgggt
     6901 gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc tgatgctgct
     6961 ggctgatcct gatgcgttcg tgtccgtaca actggcgtaa tcatggccct tcggggccat
     7021 tttctctctg tggaggagtc catgacgaaa gatgaactga ttgcccgtct tcaggtgctg
     7081 ggtgagcaac tgaaccgtga tgtcagcctg acggggacga aagaagaact ggtgctccgt
     7141 gtggcagagc tggaagagga gcttgatgac acggatgacg ctgccggtca ggacacatct
     7201 gtcagcccgg aaaatgcgct gaccggacat gaaaatgagg tggtatcagc gcagccggat
     7261 accgtgattg atacggctgc tctggtcacg gtcgtggcac tggtgacgct gcatactgat
     7321 gcacttcacg ccacgcggga tgagcctgtg gcatttgtgc tgccgggaac ggcgtttcgt
     7381 gtctctgccg gtgtggcagc cgaaatgaca gaacatggcc tggccagaat gcaataacgg
     7441 gaggcgctgt ggctgattcc gataacctgt tcgatgctgc cattgcccgc gccgatgaaa
     7501 cgatacgcgg gtacatggga acgtcagcca ccatgacatc cggtgagctg tccggtgctg
     7561 tgatacgtgg tgtttttgat gaccctgaaa atatcagcta tgccggacag ggggtgcgcg
     7621 ttgaaggctc cagcccgtcc ctgtttgtcc ggactgatga tgtgcggcag ctgcggcgtg
     7681 gagacacact gaccatcggc gaggaaaact tctgggtgga ccggatttcg ccggatgatg
     7741 gcggaagctg tcatctctgg cttgggcgtg gcgtgccgcc tgccgttaac cgtcgccgct
     7801 gaaaggggga tgtatggcca taaaaggtct tgagcaggcc gttgaaaacc tcagccgtat
     7861 cagcaaaacg gcggtgcctg gtgccgccgc aatggccatt aaccgcgttg cgtcatccgc
     7921 gatatcgcag tctgcgtcac aggttgcccg tgagacaaag gtacgccgga aactggtaaa
     7981 ggaaagggcc aggctgaaaa gggccacggt caaaagtccg caggccagaa tcagggttaa
     8041 ccggggggat ttgcccgtaa tcaagctggg taacgcgcgg gttgtccttt cccgccgcag
     8101 acgtcgtaaa aaggggcagc gttcatccct gaaaggtggc ggcagcgtgc ttgtggtggg
     8161 aaaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccggt ggcatgtcat
     8221 gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc cgatggcggt
     8281 gccgcttacc acggcgttta aacagaatat tgaacggata cggcgtgaac gtcttccgaa
     8341 agagctgggc tatgcgctgc agcatcaact gagaatggta ataaagcgat gaaacatact
     8401 gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc gacgcttttt
     8461 gatggtcgcc ccgctgtttt tgatgaggcg gattttccgg caattgccgt ttatctcacc
     8521 ggcgctgaat acacgggcga agagctggac agtgatacct ggcaggcgga gctgcatatt
     8581 gaagttttcc tgcctgctca ggtgccggat tcagagctgg attcgtggat ggagtcccgg
     8641 atttatccgg tgatgagcga tgtcccggca ctgtcagatt tgatcaccag tatggtggcc
     8701 agtggctatg actaccggcg cgacgatgat gcgggcctgt ggagttcagc cgatctgact
     8761 tatgtcatta cctatgaaat gtgaggacga tatgcctgta ccaaatccag taatgccggt
     8821 gaaaggggcc gggaccacac tgtgggttta taaggggaac ggtgaccctt atgcgaaccc
     8881 gctttcagac gttgactggt cgcgtctggc taaagttaaa gacctgacgc ccggcgaact
     8941 gaccgctgag tcctatgacg acagctatct cgatgatgaa gatgcggact ggactgcgac
     9001 cgggcagggg cagaaatctg ccggagatac cagcttcacg ctggcgtgga tgcccggaga
     9061 gcaggggcag caggcgctgc tggcgtggtt taatgaaggg gatacccgtg cctataaaat
     9121 ccgcttcccg aacggcacgg tcgatgtgtt ccgcggctgg gtcagcagta tcggtaaggc
     9181 ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc accaacgtga gacgtccgtc
     9241 gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga cgcctgccag
     9301 cacttcggtg gtgaaagggc agagcaccac gctgaccgtg gcattccagc cggaaggcgc
     9361 aaccgacaag agcttccgtg cggtgtctgc ggataaaaca aaagccaccg tgtcggtcag
     9421 tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc cggttgtatc
     9481 cggtaatggt gaacttgctg tggttgcaga aatcaccgtc accgacagtt aatccggaga
     9541 gtcagcgatg ttcctgaaaa ccgaatcatt tgaatataac ggtgtgagcg tcacgctttc
     9601 tgaactgtca gccctgcagc gaattgagca tctcgccctg ctgaaacgac aggcagaaca
     9661 ggcgggatcc agtctcaatc gacaggtgag cgtggaagat ctcgtcagaa ccggtgcttt
     9721 tctggtggcg atgtccctgt ggcatagcca tccgcagaag acaaagatgc cgtccatgaa
     9781 tgaagccgtt aaacaaattg agcaggaagt gcttaccacc tggcccacag aggcaattgc
     9841 tcaggctgaa aatgtggtaa tgcgtctgtc cggtatgtct gagtttgttg tgaatgatgc
     9901 acctgaacag gcagatgacg ccgggccagc agagcctgtt tctgcgggaa agtgttcgac
     9961 ggtgagctga gttttgccct gaaactggcg cgtgagatgg ggcgacccga ctggcgcgcc
    10021 atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta cagtacccat
    10081 tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac cgtactcagc
    10141 ctgtttttca gcgatccgga tacgcatccg ctggatttca gtctgctgaa ccggcgcgag
    10201 gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct tgccggaggc
    10261 gtccgctttg gcccggacgg gaatgaagtt atccccgctt ccccggatgt ggcggacatg
    10321 acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg agtccggtat
    10381 ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg gatgcggcca gatttgacga
    10441 gcagatggcc agagtcaggc gtcatttttc cggtacggaa actgatgcga aaaaaacagc
    10501 ggcagtcgtt gaacagtcgc tgagccgaca ggggctggct gcacagaaag cggggatttc
    10561 cgtcgggcag tataaagccg ccatgcgtat gctgcctgca cagttcaccg acgtggccac
    10621 gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg caacagggtg gtcaggttaa
    10681 ggactccttc ggcgggatga tccccatgtt cagggggctt gccggtgcga tcaccctgcc
    10741 gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg cctggtatca
    10801 gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca atcaggcggg
    10861 actgacggca gatcgtattc tggccctgtc cagagccggg caggcggcag ggctgacgtt
    10921 taaccagacc agcgagtcac tgacggcgct ggtgaatgcc ggtgtgcgtg gtggtgagca
    10981 gtttgaggcg atcagccaga gtgtggcgcg tttctcctct gcatccggcg tggaggtgga
    11041 caaggtcgct gaagccttcg ggaagctgac cacagacccg acgtcgggac tgacagcgat
    11101 ggcacgtcag ttccataacg tgacggcgga gcagattgcg tatgttgctc agttgcagcg
    11161 ttccggagat gaagccgggg cattgcaggc ggcgaacgag gccgcaacga aagggtttga
    11221 tgaccagacc cgccgcctga aagagaacat gggcacgctg gagacctggg cagacaggac
    11281 agcacgggca ttcaaatcca tgtgggattc ggtgctggat attggtcgcc cggacactgc
    11341 ccagggaatg ctggagaaag cagaaaaggc ttttgatgag gcggacaaaa aatggcagtg
    11401 gtatcagagc cgaagccacc ggcgcggtaa aacctcagca tttcttgcca atctccgggg
    11461 agcatgggag gacagagcga atgcgcaact tgggctttca gccgccacgt tgcaggccga
    11521 tcttgaaaag gccagagaga tggcagcaaa ggactgggcc gagtctgagg catcacggct
    11581 gaaatatacc gaagaggcgc agaaggctta cgaacgcctg cagacgccgc tggagaaata
    11641 taccgcccgt caggaagaac tgaacaaggc actgaaagac gggaaaatcc tgcaggcaga
    11701 ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga aaaagccgaa
    11761 acagtccggc gtgaaagtgt ctgcgggcga tcgtcaggaa gacagtgctc atgctgccct
    11821 gctgacgctt caggctgaac tccggacgct ggagaagcat gccggagcaa atgagaaaat
    11881 cagccagcag cgccgggatt tgtggaaggc ggagagtcag ttcgcggtac tggaggaggc
    11941 ggcgcaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata aagatgagac
    12001 gctggagtac aaacgccagc tggctgcact tggcgacaag gttacgtatc aggagcgcct
    12061 gaacgcgctg gcgcagcagg cggataagtt cgcacagcaa caacgggcaa aacgggcagc
    12121 cattgaggcg aaaaaccggg ggcttactga ccggcaggca gcgcgggaag ccacagaaca
    12181 gcgcctgaag gaacagtatg gcgataattc gctggcgctg aataacgtca tgtcagagca
    12241 gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag gcctcaggtc
    12301 cggctggagt gagtgggaag agagcgccac ggacagtatg tcgcaggtta aaagtgcagc
    12361 cacgcagacc tttgatggta ttgcgcagaa tatggcggcg atgctgaccg gcagtgagca
    12421 gaactggcgc agcttcaccc gttccgtgct gtccatgatg acagaaattc tgcttaagca
    12481 ggcaatggtg gggattgtcg ggagtatcgg tagcgccatt ggcggggctg ttggtggcgg
    12541 cgcatccgca tcaggcggta cagccattca ggccgctgcg gcgaaattcc attttgcaac
    12601 cggaggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc accgtggtga
    12661 atttgtcttc acgaaggagg caaccagccg aattggtgtt ggcaacctgt accgcctgat
    12721 gcggggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc agatgcggcg
    12781 ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg acggtacgaa
    12841 tggtctgcca ggtccacaga tgatgaaggc agtgtatgac atggcccgca agggtgcccg
    12901 tgatgaaatt caggcacaga tgcgcgatgg tggtctgttc tccggaggtg gacgatgaag
    12961 accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc cgtcaggaaa
    13021 gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc cgacctgaaa
    13081 acgtacagcg tgacgttttc tgttccccgt tgggaggcca cggcgctgga gtcgtttctg
    13141 gctgagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg gcggcagata
    13201 aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga gttcagcgca
    13261 gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg aatgaatgca
    13321 cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgacctgaca gaggtcggtg
    13381 gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc acctggcagg
    13441 ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat ggcaaaggca
    13501 ccagtacgcg ccccacactg acggtttcta acctgtacgg tatggtcacc gggatggcgg
    13561 aagatctgca gagtctggtc ggtggaacgg tggtccggcg taaggtttac gcccgttttc
    13621 tggatgcggt gaacttcgtc aacggaaaca gtggcgccga tccggagcag gaggtgatca
    13681 gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct tttgtactgt
    13741 ccacgccgac ggaaacggat ggcgctgttt ttccgggacg tatcatgctg gccaacacct
    13801 gcacctggac ctatcgcggt gacgagtgcg gttatagcgg tccggctgtc gcggatgaat
    13861 atgaccagcc aacgtccgat atcacgaagg ataaatgcag caaatgcctg agcggttgta
    13921 agttccgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa ctttcgcagt
    13981 aaatcccatg acacagacag aatcagcgat tctggcgcac gcccggcgat gtgcgccagc
    14041 ggagtcgtgc ggcttcgtgg taagcacgcc ggagggggaa agatatttcc cctgcgtgaa
    14101 tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc tgcaggcaga
    14161 aatgcagggt gagattgtgg cgctggtcca cagccacccc ggtggtctgc cctggctgag
    14221 tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg tctgccgggg
    14281 gacgattcat aagttccgct gtgtgccgca tctcaccggg cggcgctttg agcacggtgt
    14341 gacggactgt tacacactgt tccgggatgc ttatcatctg gcggggattg agatgccgga
    14401 ctttcatcgt gaggatgact ggtggcgtaa cggccagaat ctctatctgg ataatctgga
    14461 ggcgacgggg ctgtatcagg tgccgttgtc agcggcacag ccgggcgatg tgctgctgtg
    14521 ctgttttggt tcatcggtgc cgaatcacgc cgcaatttac tgcggcgacg gcgagctgct
    14581 gcaccatatt cctgaacaac tgagcaaacg agagaggtac accgacaaat ggcagcgacg
    14641 cacacactcc ctctggcgtc accgggcatg gcgcgcatct gcctttacgg ggatttacaa
    14701 cgatttggtc gccgcatcga ccttcgtgtg aaaacggggg ctgaagccat ccgggcactg
    14761 gccacacagc tcccggcgtt tcgtcagaaa ctgagcgacg gctggtatca ggtacggatt
    14821 gccgggcggg acgtcagcac gtccgggtta acggcgcagt tacatgagac tctgcctgat
    14881 ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg cgtattccag
    14941 attgtcctgg gggctgccgc cattgccgga tcattcttta ccgccggagc cacccttgca
    15001 gcatgggggg cagccattgg ggccggtggt atgaccggca tcctgttttc tctcggtgcc
    15061 agtatggtgc tcggtggtgt ggcgcagatg ctggcaccga aagccagaac tccccgtaca
    15121 cagacaacgg ataacggcaa acagaacacc tatttctcct cactggataa catggttgcc
    15181 cagggcaatg ttctgccggt tctgtacggt gaaatgcgcg tggggtcacg cgtggtttct
    15241 caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat tggtcgctga
    15301 tgcaaaatgt tttatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga
    15361 atgggtaaag gaagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc
    15421 acgcagttgc tgagtgtgat tgatgccatc agtgaagggc cgattgaagg tccggtggat
    15481 ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgatgg gaataccaac
    15541 atcgccggtg tcacggtggt gttccgggct ggtgagcagg agcagactcc gccggaggga
    15601 tttgaatcct ccggctccga gacggtgttg ggtacggaag tgaaatacga cacgccgatc
    15661 acccgcgcca tcacgtcggc aaacatcgac cgactgcgct ttaccttcgg tgtgcaggca
    15721 ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg aagttcgcct gctggttcag
    15781 atacagcgta atggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccacc
    15841 tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt taatatccgg
    15901 atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
    15961 tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact gttcggcgtg
    16021 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg
    16081 cgtattctgc aggtgccgtc gaattataac ccgcagacgc ggcaatacag cggtatctgg
    16141 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtatgtg ggatatgctg
    16201 acccatccgc gctacggcat gaggaaacgt cttggtgcag cggatgtaga taaatgggcg
    16261 ctgtatgtca tcggccagta ctgcgaccag tcggtgccgg acggctttgg cggcacggag
    16321 ccgcgcatca cctgtaatgc gtacctgacc acacagcgca aggcgtggga tgtgctcagt
    16381 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
    16441 caggaccgac catcagataa ggtgtggacc tataaccgca gtaatgtggt gatgccggat
    16501 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag
    16561 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg
    16621 caggccattg cccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
    16681 cgggggcagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
    16741 accgtggact tcagcgtggg tgcggaaggg cttcgccatg taccggggga tgtcattgaa
    16801 atctgcgatg atgactatgc gggtatcagc atcggcgggc gcgtgctggc ggtgaacagc
    16861 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccacgctg
    16921 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggtccagtc cgtcaccgac
    16981 ggcgtgaagg taaaagtgag ccgtgttcct gacggcgttg ccggatacag cgtgtggggg
    17041 ctgaagccgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgat
    17101 gacggcacgt atgccatcac cgccgtgcag catgtaccgg aaaaagaggc catcgtggat
    17161 aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac gccgccagca
    17221 gtgcagcatc tgaccgccga agtcactgca gacagcgggg aatatcaggt gctggcgcga
    17281 tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc ttcgcctgac cgtggcagcg
    17341 gatgacggca gtgagcggct ggtcagcacg gcccggacgg cggaaaccac ataccgcttc
    17401 acgcaactgg cgctgggacg gtacacgctg acagtccggg cggtaaatgc gtggggacag
    17461 cagggcgatc cggcgtcggt atcgttcagg attgccgcac cggtagcacc gtcgcggatt
    17521 gagctgacgc cgggctattt tcagataact gccacgccgc atcttgcggt ttatgatccg
    17581 acggtacagt ttgagttctg gttctcggaa acgcggatta ccgatatcag gcaggttgaa
    17641 accacagccc gctaccttgg cgcggggctg tactggatag ccgccagtat caatatcaaa
    17701 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattc
    17761 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc
    17821 aagataaccg aatcccatct cggtaaagag ctgctggaaa aagttgatct gacggaggat
    17881 aacgccagca gactggatga gttttcgaaa gagtggaagg acgctaacga taaatggaat
    17941 gcgatgtggg gcgtcaaaat tgagcagacc aaagacggca aacattatgt cgcgggtatt
    18001 ggcctcagca tggaggacac ggaggaaggc aagctgagcc agtttctggt tgccgctaac
    18061 cgtatcgcgt ttattgaccc ggcaaacggg aatgaaacgc cgatgtttgt ggcgcagggc
    18121 aaccagatat tcatgaatga cgtgttcctg aaacgcctga cggctcccac cattaccagc
    18181 ggtggaaatc cgccggcatt ttccctgaca ccagacggaa agctgaccgc taaaaatgcg
    18241 gatatcagcg gtaatgtgaa tgcaaattca gggacgctca acaatgtcac gattaatgaa
    18301 aactgtcaga ttaaggggaa actgtcagcc aatcagattg aaggcgatat tgtcaaaacg
    18361 gtcagcaagt ctttcccccg cacgaacagt tatgccagtg gcaccatcac ggtaagaatc
    18421 agtgatgatc agaaatttga ccggcaggtc atgataccgc cagtgttatt ccgcggtggt
    18481 aagcatgaga atttcaacag taataaccaa cagtcatact ggtattcaac ctgccggtta
    18541 agagtgaccc gcaatggtca ggagattttt aatcagtcca cgacggatgc tcagggcgta
    18601 ttttcctcag ttatagatat gcctgccgga caggggacgc tgacactgac attcaccgta
    18661 tcttcatcag gagcgaataa ctggacacca acaaccagta tcagcgatct gctggttgtg
    18721 gtgatgaaaa aatccacagc aggtatcagt atcagctgaa ttttataacc cagaacgggc
    18781 gtcagaaatg acgccttttt tattgcagaa aagcgagagg taattatgcg taaactttat
    18841 gccgccattt tgtccgcagc catttgtctg gccgtatccg gtgcgcctgc atgggcgtct
    18901 gaacatcagt ccacgctgag cgcggggtat cttcatgccc ggacgaacgt tcccggcagt
    18961 gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac actggggatg
    19021 gtgacgtcgt tcagctatgc aggagacaag aatcgccaga ttacccgtta cagcgatacc
    19081 cgctggcatg aagattccgt tcgtaaccgc tggttcagcg taatggcggg gccgtctgtg
    19141 cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg
    19201 actttctccg gggattatct ccgcgtaact gacaacaagg ggaaaaagca cgatgtgctg
    19261 accggaagtg atgacggtcg ccacagcaac acgtctctgg cgtggggagc tggcgtgcag
    19321 tttaacccga ccgaatccgt ggccattgat gtcgcttatg aaggctccgg cagtggtgac
    19381 tggcgcactg acgggttcat cgtgggtgtt ggttataaat tctgattagc caggtaacac
    19441 agtgttatga cagcccgccg gttcaggcgg gcttttttgt ggggtgaata tggcagtaaa
    19501 aatttcaggt gtactgaaag acggcacagg aaaaccggta cagaactgca caatccagct
    19561 gaaagcaaaa cgtaacagta ccacggtggt ggtgaacacg ctggcatctg aaaatccgga
    19621 tgaagccggg cgttacagta tggacgttga gtacggtcag tacagcgtta ttctgttggt
    19681 ggaaggattc ccgccgtcac atgccgggac catcacagtg tatgaagatt cccgacccgg
    19741 tacgctgaat gattttctcg gtgccatgac ggaggatgat gcccgtccgg aggcactgcg
    19801 ccgttttgag ctgatggtgg aagaggtggc gcgtaacgcg tccgtggtgg cacagaacac
    19861 ggcagccgcg aaaaagtcag ccagtgatgc cagcacatca gcccgtgagg cggcaaccca
    19921 tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggac aggccgcgtc
    19981 gtcggctcag tcagcgactt ccagcgcagg aacggcatca acaaaggcca ctgaagcatc
    20041 aaaaagtgct gccgctgcag agtcctcaaa aagcgcggca gctaccagtg ccagtgccgc
    20101 gaaaacgtca gaaacgaatg ctgcagcgtc acaacaatca gcagccactt ctgcatccac
    20161 cgcgactacg aaagcgtcag aagctgccac ctcagcccgg gatgcggcgg cttcaaaaga
    20221 ggcggcaaaa tcatcagaaa cgaacgcatc ctcgagcgcc agtagcgcag cttcctcggc
    20281 aacagcggca ggaaattccg cgaaggcggc aaaaacgtca gagacgaacg ccaggtcttc
    20341 tgaaacggca gcgggacaga gcgcctcggc tgcggcaggc tcaaaaacag cggctgcgtc
    20401 gtctgccagt gcagcgtcaa caagtgccgg gcaggcctca gccagtgcca ccgccgccgg
    20461 aaaatcggca gaaagcgccg catcgtctgc ttcaacagcc acaacgaagg ctggcgaagc
    20521 cactgaacag gccagcgcag cagcgaggtc tgcttccgca gcgaagacat ccgaaacgaa
    20581 cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaacg gctgccgcat cgtcagccag
    20641 ttcggcgtcg tcatcggcat catctgcgtc tgcttcaaaa gatgaggcga ccagacaggc
    20701 gtcagcagcg aagggtagcg ccacgacggc atccatgaag gcgacagagg cagctggcag
    20761 tgcgacggca gcagctcaga gcaaaagtac ggcggaatct gcagcaacgc gagctgagac
    20821 agcggcaaaa cgggcagagg atattgcatc cgccgtggcg cttgaggatg cgagcacgac
    20881 gaaaaagggg atagtacagc tcagcagtgc gaccaacagc acttccgagt cactggcggc
    20941 aacgccaaaa gccgttaagg ccgcgtatga cctggctaac gggaaataca ccgcacagga
    21001 tgcaacgaca gcacagaaag ggataatcca gctaagcagc gcgaccaaca gcacgtctga
    21061 aacgctggcg gcaacgccaa aggcagtaaa agcagccaat gacaatgctg agaaacgtct
    21121 gcagaaagat cagaacggtg cggatatccc tggcaaagac acctttacga aaaatattgg
    21181 tgcctgccgt gccttcggtg ggtcagtaag cacaacaaca ggaaactgga cgactgcaca
    21241 gtttatcgag tggctggatt ctcagggagc atttaaccat ccatactgga tgtgcaaggg
    21301 ttcctggtct tatggcaata ataaaatcat tactgatact ggctgcggta atattcatct
    21361 cgccggagct gtcattgaag taatggggat aaagtcagcg atgacgatcc gcattaccac
    21421 accgaccacc tccactggtg gtggaacaac taacgcccag tttacctata ttaatcacgg
    21481 aacagattat tcacctggct ggcgaaggga ctataactcc agaaataagc caacggcatc
    21541 agagatcggg gcgttaccgt caggtggaac agcagtatca tcagttaatc tgtcttcaaa
    21601 aggtcgggta accgcgctga cagacaatac gcagggggca acaggtcttg agttatacga
    21661 ggtgtataac aacggatatc caacagcgta tggaaatatc attcacctga aagggatgac
    21721 agccgttggc gaaggtgagt tactcatcgg ctggagtggt acaagcggtg ctcatgctcc
    21781 ggcatttatt cgttcacgac gggatacgac cgacgcaaac tggtcgccgt gggcgcagct
    21841 ttacacctcg gctcatcctc ctgcagagtt ttatccagtc ggtgcaccaa tcccgtggcc
    21901 atcagatacc gttccgtctg gctatgccct gatgcagggg cagacttttg acaaatctgc
    21961 atacccgaaa cttgcagcgg cttatccgtc aggcgtgatc cctgatatgc gtggctggac
    22021 gattaagggc aaacctgcca gtggtcgggc cgtattgtct caggaacagg acggcattaa
    22081 atcgcatacc cacagcgcca gcgcatccag tacggatttg gggacgaaaa ccacatcgtc
    22141 gtttgattac ggcactaaat ccacgaataa cactggtgcg catacccata gtttaagtgg
    22201 cagcacgaat gcagctggta atcacagcca tagagatggc cgtcgattta accccagtgt
    22261 ttttaaagat acttatcaat atggttatac aagctcaggt caaaatacct ggggtgtaca
    22321 aggctcagta ggtatgtcta cggggtggtt agctaatacc agtacagatg gtaatcatag
    22381 ccactcactg tccggcacag cagcatctgc aggtgcacac gcgcatactg tcggtattgg
    22441 tgctcatacg cactccgttg cgattggttc acatggacac accatcaccg ttaacgctgc
    22501 tggtaacgcg gaaaacaccg tcaaaaacat cgcatttaac tatattgtga ggcttgcata
    22561 atggcattca gaatgagtga acaaccacgg accataacaa tttataatct gctggccgga
    22621 actaatgaat ttattggtga aggtgatgca tatattccgc ctcatacagg tctgccagca
    22681 aacagtaccg atattgcacc gccagatatt ccggcaggct tcgtggctgt tttcaacagt
    22741 gatgaggcat cgtggcatct cgttgaagat catcggggta aaacggttta tgacgtggct
    22801 tccggcgacg cgttatttat ttctgaactc ggtccgttac cggaaaatgt tacctggtta
    22861 tcgccgggag gggaatatca gaagtggaac ggcacagcct gggtgaagga tacggaagca
    22921 gaaaaactgt tccggatccg ggaggcggaa gaaacaaaaa acagcctgat gcaggtagcc
    22981 agtgagcata ttgcgccgct tcaggatgct gcagatctgg aaattgcaac ggaggaagaa
    23041 atctcgttgc tggaagcatg gaaaaagtat cgggtattgc tgaaccgtgt tgatacgtca
    23101 actgaacagg atattgaatg gccagcactg ccgtagggta aaacatataa attctataat
    23161 tagatgtatc tttccattta cggcaaggaa ggtggcttgg aagacgtaaa gcatctcaca
    23221 ccgagattat tttttatatg tcaggtgtct gaagttttgc tttggctctt aaaatggttt
    23281 gccgcgaggt tttgaattcc cgggcaatgg cacttatact tacacctgac ttaattcgtt
    23341 cgaataccac ctgtttctgt tcttcattta acacaggtgg tcgaccaaaa cgtttccctg
    23401 cgccccgggc tcttactatc ccggaatgag tgcgttcaag taaaaggtct cgttcaaatt
    23461 cagcgactgc tgaaattact tgcatcatca tttttcctgt tggactggtc aggtcaatgc
    23521 cccccaatgc taagcaatgc actctgatac ctgtttcggt cagttgttcc actgttttcc
    23581 tgatatccat tgcattacaa ccaaggcgat ccagttttgt cacaatcaat tgatcaccac
    23641 atttcaggcg agcaagcaac cggttaaaac caggacgctc actggttgct gctgagccgc
    23701 taatgtgttc ttcgattatt tgctgaggtt tgattttaaa acctgcactt tcgatttccc
    23761 ggcgttgatt ttcggtggtc tgatccagcg ttgatatccg
//
LOCUS       DAPPNJ010000061        23738 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000061 DAPPNJ010000000
VERSION     DAPPNJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.061"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            271..2943
                     /gene="rcsD"
                     /locus_tag="RPM73_001959"
     CDS             271..2943
                     /gene="rcsD"
                     /locus_tag="RPM73_001959"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDX6520324.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2960..3610
                     /gene="rcsB"
                     /locus_tag="RPM73_001960"
     CDS             2960..3610
                     /gene="rcsB"
                     /locus_tag="RPM73_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDX6520325.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3810..6659)
                     /gene="rcsC"
                     /locus_tag="RPM73_001961"
     CDS             complement(3810..6659)
                     /gene="rcsC"
                     /locus_tag="RPM73_001961"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDX6520326.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6934..7710)
                     /locus_tag="RPM73_001962"
     CDS             complement(6934..7710)
                     /locus_tag="RPM73_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDX6520327.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7715..9364)
                     /locus_tag="RPM73_001963"
     CDS             complement(7715..9364)
                     /locus_tag="RPM73_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDX6520328.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(9365..13879)
                     /locus_tag="RPM73_001964"
     CDS             complement(9365..13879)
                     /locus_tag="RPM73_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDX6520329.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13903..14526)
                     /locus_tag="RPM73_001965"
     CDS             complement(13903..14526)
                     /locus_tag="RPM73_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDX6520330.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(14523..16211)
                     /locus_tag="RPM73_001966"
     CDS             complement(14523..16211)
                     /locus_tag="RPM73_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDX6520331.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(16360..18987)
                     /gene="gyrA"
                     /locus_tag="RPM73_001967"
     CDS             complement(16360..18987)
                     /gene="gyrA"
                     /locus_tag="RPM73_001967"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDX6520332.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            19134..19856
                     /locus_tag="RPM73_001968"
     CDS             19134..19856
                     /locus_tag="RPM73_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDX6520333.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(19984..23718)
                     /gene="yfaL"
                     /locus_tag="RPM73_001969"
     CDS             complement(19984..23718)
                     /gene="yfaL"
                     /locus_tag="RPM73_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDX6520334.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGFEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         5697 a         6494 c         5798 g         5749 t
ORIGIN      
        1 gaaaaactat aacgcttgtt aactatttca caaataatta acatcagcat aatttccagc
       61 aatcttcgtt tatttgcaat taatttcgtt gggctttttg taggttattt gtacagcaaa
      121 atggcgcttg tacatctatt tcccccatac aggatgataa atatcacggg agaatagaga
      181 atcatcaatc aggtaagagt ctggaatttc acactgtacc ctttatactg ccctatcact
      241 tcgcgaagtt ttaacaggtc ataaacacga atgcgtcaga aagagacaac ggccacgacc
      301 cgcttttcac tcctaccggg gagcattacc cgcttctttt tactgttgat cattgtgtta
      361 ctggtgacga tgggggtaat ggtacaaagc gccgttaacg catggctgaa agataaaagt
      421 taccagattg tcgacattac ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg
      481 acctggcaga tctacgacaa cattgccgcg acgacctccc cctcctccgg cgaaggttta
      541 caagagacgc gcctgaaaca ggatgtctac tatctggaga aaccacgccg caaaacggaa
      601 gcgttaatct ttggctctca cgacaactca acgcttgaga tgactcaacg gatgtccacc
      661 tatctggaca cattgtgggg cgcagaaaat gtaccgtggt cgatgtatta cctgaatggt
      721 caggataaca gtctggtgct gatctcaacc ctgcccctca aagatctcac ctccggattt
      781 aaagaatcga ccgtcagtga cattgttgat tcacgtcgtg cagaaatgtt gcaacaggcc
      841 aacgccctcg atgaacgcga aagtttttct aacatgcgcc gcctggcctg gcagaacggt
      901 cattacttta ccttgcgtac cacgttcaac cagccgggac atctggcaac ggtcgtggct
      961 tttgatctgc cgattaatga tttgatccca ccgggtatgc cgctggacag tttccgcctt
     1021 gagccagaca cgacggcaac gggagacaat gataatgaga aagaaggaac ggatagcgtc
     1081 agtatccact ttaacagtac gaagattgaa atctcctcgg cactcaactc taccgatatg
     1141 cgcctggtct ggcaggttcc ttatggcacc ttattgctgg atacgttgca aaacattctg
     1201 ctgccactgc tgctgaacat cggtttgctg gcgctggcgt tatttggcta taccacattc
     1261 cgccatttct ccagtcgcag tacagaaagt ctacccaaca cggcggtcaa taacgaattg
     1321 cgcattttac gggcaatcaa tgaagagata gtctcactgc tgccgctcgg cctgctggtt
     1381 cacgatcagg aatcgaaccg cactgtcata agtaacaaaa ttgccgatca tttgctgccg
     1441 catttgaatc tgcaaaacat caccaccatg gcggaacagc atcaggggat tattcaggcg
     1501 acgatcaata acgagctgta tgagatccgc atgttccgca gccaggtcgc gccgcgcaca
     1561 caaattttca ttattcgcga tcaggatcgc gaagtgctgg taaacaagaa actcaagcag
     1621 gcgcagcgtc tgtatgagaa aaaccagcag ggccggatga cctttatgaa aaacattggc
     1681 gatgcgctga aagaacccgc acagtccctg gcggagagcg cggctaaact caacgccccg
     1741 gaaagcaaac aactggcgaa tcaggcagat gtgctggtgc ggctggtcga tgaaatacag
     1801 ttagcgaaca tgcttgcgga tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag
     1861 gatttaattg atgaagttgt gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa
     1921 ctgctgatta acaatcatct gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta
     1981 cgacgtattt tgctgctact gatgcaatat gccgtgacct caacgcaatt gggaaaaatc
     2041 acccttgagg ttgatcagga tgagtcctcc gaagaccgcc tgacgttccg cattctggac
     2101 acgggagaag gcgtaagtat tcatgaaatg gataatttgc acttcccgtt tatcaaccag
     2161 acccaaaacg atcgctatgg caaggcggac ccgctggcat tctggctgag cgatcaactg
     2221 gcacgtaaac tgggcggtca tttaaacatc aaaacgcggg atgggcttgg tacacgctac
     2281 tctgtgcata tcaaaatgct cgcagctgac ccggaagttg aagaggaaga agagcgttta
     2341 ctggatgatg tctgcgtaat ggtggatgtt acttcggcag aaattcggaa tattgtcact
     2401 cgccagttag aaaattgggg tgcaacctgt atcacacccg atgaaagatt aattagtcaa
     2461 gattatgata tctttttaac ggataatccg tctaatctta ctgcctctgg cttgctttta
     2521 agcgatgatg agtctggcgt acgggaaatt gggcctggtc aattgtgcgt caacttcaat
     2581 atgagcaacg ctatgcagga agcggtctta caattaattg aagtgcaact ggcgcaggaa
     2641 gaggtgacag aatcgcctct gggcggagat gaaaatgcgc aactccatgc cagcggctat
     2701 tatgcgctct ttgtagacac agtaccggat gatgttaaga ggctgtatac tgaagcagca
     2761 accagtgact ttgctgcgtt agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg
     2821 ctaaatctgg tacccggcaa gcagttatgt gaaacgctgg aacatctgat tcgtgagaag
     2881 gatgttcctg gaatagaaaa atacatcagc gacattgaca gttatgtcaa gagcttgctg
     2941 tagcaaggta gcctattaca tgaacaatat gaacgtaatt attgccgatg accatccgat
     3001 agtcttgttc ggtattcgca aatcacttga gcaaattgag tgggtgaatg ttgtcggcga
     3061 atttgaagac tctacagcac tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat
     3121 taccgatctc tccatgcctg gcgataagta cggcgatggc attaccttaa tcaagtacat
     3181 caagcgccat ttcccaagcc tgtcgatcat tgttctgacc atgaacaaca acccggcgat
     3241 tcttagtgcg gtattggatc tggatatcga agggatcgtg ctgaaacaag gtgcaccgac
     3301 cgatctgccg aaagctctcg ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt
     3361 ttctcgcctg ttggaaaaaa tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa
     3421 agagagtgaa gttctgcgcc tgtttgcgga aggcttcctg gtgaccgaga tcgctaaaaa
     3481 gctgaaccgc agtattaaaa ccatcagtag ccagaagaaa tctgcgatga tgaagctggg
     3541 tgtcgagaac gatatcgccc tgctgaatta tctctcttct gtgaccttaa gtccggcaga
     3601 taaagactaa tcatctgtag gccagataag acgcgttagt gtcttatctg gcatttgcac
     3661 cgattgccgg atgcagcgta aacgccttat ccggcctacg attcccatta tttcaacaga
     3721 ttacattaaa gtaggccaga taagacgcgt cagcgtcgca tctggcattt gcactgaatg
     3781 ccagatgcgg cataaacacc ttatccgtcc tacgattccc gcgatttcct gaccctctcg
     3841 gcatataccg tcagcgtctg ttttatcaca tccagcgtta ccggcttcga caggcagctg
     3901 tccataccgg actccagaca ccgctgcttc tcttcagcca acgcattagc agttactccg
     3961 attaccggca acgtcagtcc caactgacga atgcgttgcg tcaagcggta accatccata
     4021 tttggcatgt tgacgtcgct aagcacgata tcaatatgat tcttgctaag tacattaagc
     4081 gcatcgacac catcattcgc ggttttacat tgatagccca acgatcccaa ctgatctgcc
     4141 agcagacgtc ggttaatcgg atgatcatcc acgaccagaa tcatcatatc gtcattatcg
     4201 ctgaccgctt tgtccgtcga cggcagagcg ttagcaggat cgtcgctctc catctcgatc
     4261 aaataaatac gcgccaacaa tgccggtagc tcatgcgggg cagccacact gtgtacccac
     4321 tcccctggcg ctttctccag cggaatacca atatggcgac gacagaaggt cactaccgct
     4381 ctaccctgcc attttttact cactacctcg tcagtaatta acacatcttc gggagtcggt
     4441 tcctgccctt cgtatgttgt aacgacgatg ccgctgcgct gcaaactggt ttccaggaac
     4501 tgacagagcg acgcattgcg gaccgccagc cagcagcgtt taccactcaa cccttccacg
     4561 ccttttttct gcgggtactg agcgccgtac aacggaatac gcacggtaaa ctggctgccc
     4621 attcccggtt ctgaatctac cgagatatcg ccgtccatca tgctgatcag tttttcacaa
     4681 atcgccagac ccagaccggt cccctggaag tttcgctgta cgcccgttcc gacctggaag
     4741 aagggatcaa acaagcgcac cacttctttc gccggaatcc ccacgccggt atcgcgaaca
     4801 cggatagaga gataatcgcc atcagcgcgg acatgcaaaa ctatacagcc ggtatcggtg
     4861 aattttatgg cgttactcaa caggttggag atgacctgct gtaaacgcat cgggtcgcca
     4921 tttaaggcca ctggcacatc cggttcaata aagcagtaca agcctaactg cttgcgtacc
     4981 accagcggta aatagttggc ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc
     5041 ggttcgatct tcaactgttc cgattcaatc ttcgagaaat cgagaatatc gctgataatt
     5101 ttcaacaaca ggctggaaga gttgttcatt gccgtcacca gccgatcgac gcctttcggt
     5161 aactctttgg tttgcaacag atccaggtta ccgataatgc catacagcgg cgttcgcagc
     5221 tcatgactga cggtggcaag gaacatcgat tttgactggc tcgcctgttc cgctgcttgt
     5281 gccatctcct gcaacgactc ttccatcttc acgcgcgaag aaacatccac cagcacacaa
     5341 atggccacgt tttcattacg atagcgcgaa tggacgaagc tgatttgcag attggtattg
     5401 ttgctggtca ggacatcaac aaaattgacc tgctgcccac agataatttg cgtcagtcgt
     5461 tggcggtcct catgcgtaag catattgaga taggtatgcg ccagttcgtt acttaaaata
     5521 ttgacgccat cagcggtacg caaaatgcag atacccactg gcgcggaggc gacaatcttg
     5581 cgattgaact gctcatgttc ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata
     5641 cgtcgctcgt acatccgcgc gagagtaaac aatgcagctc cggcaagcac attcagcaaa
     5701 attgcgttaa ggatcaacat gcgaatgcgt tccagcacct tatcaaccgg caccgaatac
     5761 acgatgctta gcgatgaggg tggcagattt ttcttcagca ccagctcccg gaacccttcc
     5821 gtatagccaa accaggagcg ttcctgcatc cagcgaggat cgcccttaat tttactttct
     5881 ggtccggtaa gcgaaatcag ggtatgacca ttttcatcaa gaatggtaac ccccatcggc
     5941 aacgtacccg gtaagaaaaa gttctccatc cggatggtct gctcgacacc caaaagcgcc
     6001 tgcaaccggt tcgccagata aactggcgtc aacgcgtaaa aatacccgac gccagggcgc
     6061 ggaccttcgc tgatccagta gaggttactg ccgctatcat cttgtggtgc atttcgatat
     6121 ttattgatgc gttcatgcaa agctttcaac gcggtatcgc gttccactgg catatcacgc
     6181 agaccgaaat tggccatgca gaggttatca ctgccgatta aaaatacccg gttgagatcg
     6241 taagccgcag aaaaattatc gcgccagtag cgcataaacc acgccaatga ctccagagaa
     6301 cctcgccagg tgttactcat tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca
     6361 ggcacatccg cctgcgtttc tcgtccacgc ggggaaagca caccgttttc tgccgataag
     6421 cgattttcgg cgatgtactt cagctctttc atcacatcag aggtgcgttg aataaagcgc
     6481 tgagcctgat cggaactcag attaaattcc tgacgaattt ccgattctcg ctgatgtaac
     6541 gcattaacga tgtaaaaaac ggatgaaaaa gcaatcaaca gccagagcac taacgccaat
     6601 gctctgaaca tgtagcgcga ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa
     6661 ggggcgaagc tccgcctcag gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg
     6721 ttaccgcaat gttctgtcac cattgcaata atgcgtatcg cttagaaacc attgccttaa
     6781 ccaggaaagc cggagtagat ggacagttct cagtacgaaa aataagaaat gaccaatctg
     6841 ccgacgcttg ttaatggtta tatcctggcg tcggcagatc gcccagcagt cttatctgaa
     6901 aaaagagaga gccaggttgt gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt
     6961 cagctcgcca gcattacgca tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc
     7021 ttcatcggtg atcagcgtca gttgggcggt cgtcaattcc gtttcgctgc gcccaccata
     7081 gtagttgata tacacctgat agcgcccgtg aactggtgct ggcatggcga aaatctcggg
     7141 tccgtacccc gtcgtgacat ccatatccag tgcaccactg tttttcagca cggtgttacc
     7201 gtaccaggcg tgttcgccat cgggcgtgac aacatggaga tcgagatcgg tattgtccgt
     7261 atcccacgag agaaccagcc gtaaacgtgc acgaatcgtt cctgtacccg gcgttgagta
     7321 aaactgcatt ttttgtcggc tttgcccatc cgggctgatg acctgcacgc tgttgctgcc
     7381 ttcagtgaaa atataagggc gtgcaaatga accatcggat tcaactctct gcggcatact
     7441 ggcaccgttg accaccagcc ggccctgctg aactttaccc gccgcagcat aattctttat
     7501 tttgccgcgg atctgcgctg agatattttg atcatctgcc atgttgacgg acgacgccgg
     7561 gtaattgatc gattgtgaaa aactggcatc ttcgccctct gccggatgcc agccagagag
     7621 cggtgcgtca atctcgactt cctgcacacc ttcactgtga gcgacaggcg aaagtgccac
     7681 cagtaacaac ggaagaaaaa tctttcgcat agcattagtc cattaataat tgtcgggtca
     7741 gcgtttcgat atagttctca tcaagcccgg tgggataacc atcaaaggcc aggtgcagat
     7801 actcatgggt taaatccagc cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt
     7861 tctgctgccg atcggtatag gggaaacctg agactaaacg acagaccgca aacacgtctg
     7921 gttcgttgta ccccgtctcg ccttgtaata tacgccgcca ctgcggcatt tttttcgcca
     7981 gccaggcttt cgctttgggt aataactggc aggttgagcg tggagctccc cagcggctaa
     8041 ggctgttgtc gggataagca aacgcgagta tctggtcgta acgctccccc tgccccgcct
     8101 gggccgtggc ttgccgccag gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa
     8161 ccggatcgcc tgcatagatg agatcctgcg tccaggcggt catcgttcgc gcccccgtgg
     8221 tcgctggcga agcagaaaca cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcgc
     8281 cttcccgatt ggcgttttgc tgtaaaaaag tgcgaatcgc cacggtcatg gctttcgctg
     8341 cctcaggggg cgtacttttc gcctcgcgat ccagaactct ggcaacgtac tcttcacgat
     8401 cgagatgcga ctgcaatttc agtttgccct tttcgctcag taaggtcgtc tcaccgtggc
     8461 tgacaaacgt aatgtggtta ccattagtaa acgtcacacg atagcgccca ttaagtacgc
     8521 cgggttttac tgccgtcgta ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa
     8581 acagctccac ctctacacat tgcccactgg caacctgcgt cggcactggc aaaacccgat
     8641 taagcaccgt cgcataacgg gttagtaccg ttttgctggt gccactgcca gtaacccaca
     8701 gcggtgtacc atcggttaac cagccagcaa aaccaccctg gcggatttcc tgtttatcat
     8761 cggcaaacca gctccaggtt ttcacccgta cccgactacc gagcatggat gcgacaccga
     8821 ttttcgcctc gtccagcacc acatccagta agacttcctg agctttgttt tgcgcaggta
     8881 acgtcgctaa tgaatcgagc agcgatttaa ccgttaccga ggtttctggc ttcagcgttg
     8941 taagagacgc taaccacgca ggtgcctgac gctgttgcca gtattgcccc cacacatctg
     9001 cgccaatatg caaccgctgc ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat
     9061 cgcgcgttat gctctcccca gcctgacaac aataaacctc ttccggggag tttccgcgac
     9121 actggtaggg ctgctccggt tgatgggtat cctccagcca ggcgtaaaca tacagtttcc
     9181 acaaactccc taacggcgtg gtgagcgagt caggtaatgc tgagactttg gtcacgcctg
     9241 atgacgacag ctgatacagt tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg
     9301 tctcctccgc aagcgttggc agagtaacca acgccaatag ccagacgatt ctgcgccagt
     9361 tcacttattt cacctgcatc ccggtccatt cactccctgc cgcaacactt tgctgcgcag
     9421 gtgcatagga acgcacataa cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc
     9481 gcagcaaatg gcggaaagtg accgttcccg tcagttcttt caccggcacc atataggcca
     9541 gttcgcccat ttcattacgc gctttttcca gcaattgccc ctgctgtttc gcggcgttgg
     9601 gtttattgac tgaaatgccc catgttgtgc gctcaacgtc ggctcccggc gggagcggta
     9661 cttccacctg accatagcgc agaactgcat cctgctcgct ggtaagcgta atttcatcga
     9721 gatacagcgc atcgctgtca atctcattgc tggtcaccgg ttgcagaata aagctcatct
     9781 cttcttcacc agggataagc cgatacaact ggcgttcaac ggtcaccgga atgttacttt
     9841 gttgagccat ttttgccggc tcacgccagc ggacctgcac attttgcggc gataattcgt
     9901 caccaaaaga gagaatgtcc ggcacgccct gaccaaccca acgccagtct tcgccccctc
     9961 cagttaactt atgtttagcc catgcgcccg caggcgcagg caaaacaact ggaggcattg
    10021 tcgccatata tttcgccagc cagttcatgg ccagcgcgcg ctcaatagtg gattgctcag
    10081 cggtcaaacc acttaaaata gcggccgtct gggtagcgtc accaccgcta ttgagcagca
    10141 caaccgtgtg cgccagcggc tgattgctgt ttgccgcttt atcccacgca tattgctgaa
    10201 cctgtggcat caccgtggac ttcaactgcg cttttttcag caacgtcatg gttaagacgt
    10261 ttgccaccgc atctgccagt ggagattccg gtgtatcgag gatcaggctg tcattgatat
    10321 cgcggatgtc ttcctcagag aaatcttcat ctttagttcc gcgccgggcg atagcttcat
    10381 ccaacccttt caacaacgtt ttgcacggca gattcatctc ctgtgcccac gccagcacca
    10441 gcgcccgatg caataacggc atgttatccg cctgctcggc ataactgtcg agcatatgct
    10501 gccagtattc cggttgttgc gttacgccga gcgcctggct ggcctgccag tcggcgtacc
    10561 atgcccatgc cgtaaggaag gcgtcaccat tgccatcttc accccaccag gtaaagcgcg
    10621 ctccgggccc cgccagttgc atcagccgca gacggttatc ctgaatcatc tgacgaatgt
    10681 cgttagcggc ggcactttga tgatcggcaa gcgaacgcca ggcgagactg agcgggatca
    10741 gacggctacc ggtgttgatc acgccacccc atggtccatc gactaacgca tcaagattgt
    10801 tgcggaaaat ctcctgcggc gtttcactac tttgtagccg gatattactc gcctgctcgg
    10861 gcaacatcag cgcgttctcg ccaccgccga gcatgacatt tttctgctgt tcaacgggcc
    10921 agctgttgtc cacaaaagac agttttgtgc taatgctgtc ctgcacttgc ccattttgtt
    10981 gcagttctgc acttaacaag ccagattgct gaatgttctg cgtcagcgaa atataattcg
    11041 cccctttgtg cagcgtcagc gtctggcgca tctcagcgcc tgcaaattta gtcaccagcg
    11101 ccaccggttc gttatcctgc tgactgaaga taaacagtcc tgccgccggt ttgtcgccca
    11161 cgcgatacac cgttggcata ctccacttca tgtagagatt tttttccgaa cgcagataag
    11221 cacgcccctg cccgaccagc ccgtcgccgt tcatcccacg cgcggtgata cgccagcggg
    11281 ttaacgaatc aggcatcagg aacgtgaaat acgctttgcc ttgtttatcg gttgtgagtg
    11341 acggcatcca tgccgcggta tccacctctt cacgccgtgg acgttcaagc atttttactc
    11401 gccgctcgct gcggttagtt gcgccaggcg caaccggctc gctggagagc gcctggtcgt
    11461 agctgataaa cgacagactg gagctggtac gcacattgtt acgccccagc ggatagaaaa
    11521 atttgccgat attcggcgcg atttctggtt gcagcgcgta gatcatttca tcgaccacgc
    11581 ctaccgttag ctgcgcagaa acaggtttac ctttcagcga cgaggttaat tcgacattga
    11641 ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca agctgaggaa
    11701 cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta tacagcaccg
    11761 aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca tactgggtat
    11821 cgtttaaacg ttgtaacgtc agccagtttg ccggatgcga aagcagcgac tgctgttcca
    11881 cgcgatcgcg ttccagcgtc aataatgctt catcaattgg ctccggaaag gtaatcagca
    11941 tcttcgcagt ttcgcctggc tggtacagcg ttttatccgc cacgatatct accgtacccg
    12001 tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga attaagccgt
    12061 ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg gtaaaggatt
    12121 tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc aaccattcat
    12181 acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca accgactcgc
    12241 cgctattact gtattgtgcg gcagtactta atgagtaatg cgccagaccg cgttcaatga
    12301 ggatctcttt ggtggtggtg acacgatacg ccgcgccgtc actggcggag acggttaaca
    12361 aataacggct cggtttatcg gcggcgggga gatttaacgc cacatgaccg ctggcgtcgg
    12421 acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc aaatcgttac
    12481 cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt tttaccggct
    12541 cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt ttgaactctt
    12601 ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc acgcgaaaac
    12661 tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg gcaacggcat
    12721 tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg acatcgacgg
    12781 tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg ctgacgatgg
    12841 gggatgaatg caacggatcg tggaactcgc ggcccatcac tttaacatcg acacgatcgc
    12901 ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag atttcgcttt
    12961 cgtagaagaa gttctcggag acaaaaacac cgccttcagc atccttaccc agaatgtatg
    13021 aacgttctgg cgatatatgt tgtaactgca aggtaccgct gtcatcggtc acaccgcggg
    13081 tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct tcaccctgtt
    13141 ttttacccgc ggtccacacc agaagctcgt tgcctgacac tttgctaagc gccacggtat
    13201 cggaaacaaa caccaccgtc gtcgcccgat acccaccaac catcgcctcg acgaggtaca
    13261 gtcccggctc ttgttggccg agaggaatat aaatgttacc cggctgcggc gagatgaaat
    13321 tgctggatgc gccttccagt tttacccctt gctgcggctc gaccggttta gcctgccata
    13381 gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac tggttgttct
    13441 gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt aatgcctgag
    13501 tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga gatttgccgt
    13561 accagttatc ccacagccag gtcagcgtat tgttcagccc gtcgcccaga tattgcggtt
    13621 gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg tcaggaatac
    13681 gatacaggcg aacgtccacg ccgccgtact cttccatctg atagcgccga taatcacgcc
    13741 ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg tcagcaagca
    13801 aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa tcatcagcat
    13861 tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg ccagaaaatt
    13921 taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc gggtgtcctt
    13981 ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct
    14041 tccggtgtgg tagatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg
    14101 atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcctggccaa taaactggct
    14161 gttgtactga atcaaattaa tcgcggtcac gtaggggccg gttttcccgt tcccctgatt
    14221 ccagttttgc gccagttgac gctgtccagg tgttagcgtc atctctggcg gcaaatactg
    14281 gctagagaaa ccgttacttt ttagccattt actatcgtga actttcagcg tctcgttcgc
    14341 cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg gcccctggcg
    14401 gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt gttcgacatt
    14461 cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg
    14521 cctcatagcg gctgccaggt tatgggtagc cactgccagg cggcaccggg ttccatctgg
    14581 gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa
    14641 gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt caacgtttcg
    14701 ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat gccatcagtg
    14761 ggtattacat ccaccattgc cgggcggttt ttattcagtg tttgcagggc gttattaacc
    14821 agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga cacgcggaaa
    14881 aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata ctgaccgtat
    14941 cgggaactca cttcgcgacg ccagatttgc gcttcgccct gctgagtctg gcttatcggc
    15001 aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccct
    15061 ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg ggtttgcaat
    15121 tttgagtctg cataccagca cagccccgcg gcaccgtcta acgccccatt caacttgtcg
    15181 ttttcctgac tgatgtgcga aagcatctct tcggcaatac cgtgtgaata cggcaccacc
    15241 acacagaagc tggccccggc aggcatactg ttccagaccg gcgtaaaatc gaaactggta
    15301 tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc gttacccatt
    15361 tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc cagcaacctg
    15421 gcgctgacca cgatgcgctg gcgtactggc gttttttcag cagcacgctc ttccaggcca
    15481 aagcttgctt gccagcgttt tttgccgctc aacaaatcac ctgcgatcgc cgtggcttcg
    15541 gtatcctgct gatcatcttt aaacaacata tccgtgctgg aaaacaccag catcttgtcc
    15601 tgataagtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata aacaggaaca
    15661 gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct gtcgctggta
    15721 gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat cagcaccata
    15781 taatgtgaaa gatggccttg tttatcgtgc cacagcgctg cctgcgcggg ctgatctaag
    15841 agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat acgacgaatg
    15901 ctgccttcaa tgcccagacg atcggcatga ttctgataat agaaaacgaa atcttcgctc
    15961 agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct cagagagtcg
    16021 ctgtcgagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc
    16081 ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa
    16141 agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt cgcctttttt
    16201 tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg cacagccaaa
    16261 ctttaccgtg ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa
    16321 acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc
    16381 gtcaacgtcc acttccggag cgatttcatc gtccccttcc gcggcactgc catcgatggt
    16441 atccagatct tcctcgtcaa ccggttcagc aacacgttgc agacccacta cgttttcatc
    16501 ttccgcagta cggatgagga tcacgccctg ggtgttacgg cctacgatgc tgatttccga
    16561 aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc
    16621 tacctgtacc gcgccaacca ctaaaccatt acgttcagta accttaatgg agataacccc
    16681 tttcgtcgca cgcgacttgg ttgggtattc cgccactgcg gtacgtttac cgtaaccgtt
    16741 ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc
    16801 gccttcacct aagcgaatac cgcgaacacc ggtggtgttg cagcccatcg cacggacaga
    16861 agactcttta aagcgcacca ctttaccttc agcggagaac agcattactt cgtcttcgcc
    16921 gctggtcagg tcaacgccga tcagctcatc gccgtcaacc agtttgatcg ccactttacc
    16981 ggcggtacgc agacggttga actcggtgag gacagttttc ttcacggtac cgttagcggt
    17041 cgccatgaag actttcacgc cttcttcaaa ctcggtcact ggcaggatcg cagtgatacg
    17101 ttcgtcctgc tccagcggca gcaggttgac gatcggacga ccgcgcgcgc cacgagtggc
    17161 ttccggcaac tgataaactt tcatcgaata gacgcgacca cggctggaga agcacagaat
    17221 atggtcgtga gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc
    17281 ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg gctgatactt
    17341 aacgtagccc tggtgagaga gcgtcactac cacatcttcc tgagtgatca gatcttccag
    17401 gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga actgttcacg
    17461 aaccagctcc agctcttcac ggatcacttc catcaaacga tcggcgctac caagaatacg
    17521 caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc
    17581 aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag
    17641 gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc
    17701 gtcgccagca cgttcgagca tcgcggcaac gttgcccagc tgccacggat tagcaaccag
    17761 cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcgat
    17821 gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag
    17881 ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg ccgcgatgat
    17941 gtctttcaag ttcatgatct tcggctgacc atggtgcaat gccaccatgt tgataccgaa
    18001 agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc
    18061 gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct
    18121 gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt
    18181 gtttacctga tacggaattt cgtggacgat aatggtttca cgaccggttt tggcgtcaac
    18241 ttccacttct gcgcgagcgc ggatatacac cttgccgcga ccggtacggt aagcttcttc
    18301 aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg ggatgtgttc
    18361 catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac
    18421 ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc
    18481 gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc
    18541 atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat
    18601 tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc
    18661 gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac
    18721 gatcgtgtca taaaccgccg agtcaccatg gggatggtat ttaccgatta cgtcaccaac
    18781 gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta
    18841 aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac
    18901 aatgaccgac atcgcataat ccagatagga gctcttcagc tcttcctcaa tgttgaccgg
    18961 tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc
    19021 aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac
    19081 atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg ctaatgaatg
    19141 ccgaaaaatc gccggtaaac cataacgtag accacgaaga gatcgctaaa tttgaagccg
    19201 tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc attaacccgc
    19261 tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg
    19321 tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg
    19381 gtctggatat gggctttgag ccattgcagg tggcaaaact gcacgcgctg gaaagcggca
    19441 ttcaggtgga ttatgtgcag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt
    19501 atgatgtggt gacctgcatg gagatgctgg agcacgtccc cgatccgcag tcagtggtca
    19561 gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca
    19621 acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc atggtgccca
    19681 aaggcacgca tgatgtgaag aagtttatta aaccggcaga actgcttggc tgggtagatc
    19741 agacaagcct taaagagcga catataaccg ggctacatta caacccgctc actaatactt
    19801 ttaaactcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt
    19861 gttgtttggc cgagccaatg cctgatgcga cgcttgccgc gtcttatcag gcctacaaat
    19921 gctccccgta ggccggataa ggcgtttacg ccgcatccgg caaccgtgcc gactagacag
    19981 tgattaccat ttcaccgtca tcgacaaaaa ccctgccatc tgggcaaaat catcactccc
    20041 tttctgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc ccgtgactcc
    20101 cacttttatt tcaccccgtt gcttcaccgc atcgtcactg atagtgctgc catcctcttc
    20161 aatttccgtc gaatggggat cgtgataata attcagatct aatgttggta tgacatgaac
    20221 agcggtacgc cattcgctgt gtaaacccag ccgcatctga atatcatcac cctgcgattg
    20281 tgacacgcgc gcacggttag cggcggtaaa atcatcctgc tgcacgccct gataaatcac
    20341 ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc cggcttccag
    20401 cgaggcgata atccccgacg agtggtaatg atctgtgcca tcgtcttgtt cggaaacatc
    20461 attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct tctgattacc
    20521 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
    20581 agttccggtc atattcgagc ggctctcgcc ctgattatca ctgtagccac caaccgcccc
    20641 aagcatccac tcgccatcat cgccccagcg cccgctaaac aggtcaccgc taagctgcac
    20701 cgtagaagtg tcttcatgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
    20761 gataacacgt aaattcagcg tctgaccatt gccacctgca tgatcgcgtc gctccatcat
    20821 aaacgcctga tttgccgccc gcagattatt gagataaccg ccaactttgg cattcagcac
    20881 cggctggtaa gcggggattg gatcgggtgt tgggtcagga tcgggtgttg ggtcagggtc
    20941 tggatcaggt ggcaatggtg gcgttacttc ttgcgatcgc agataccagt cgttgttatc
    21001 ttccaccagc gtgtagtcat atgctcccat attgacgtag ccgctgcctg ccagactgaa
    21061 cttcgcattg ttttgaaact gcgtgggatc ggctgcgaaa tcaaccactt taatgcctgt
    21121 cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt tgccagcagt
    21181 attaccgttc aacaccaatt gatcgcttac cgagtcatcg ccgtttaatt cgctatcgag
    21241 cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc cagcgacgcc
    21301 attttgcagg ctaaccgcac cggacgtatt ggtcagatca ccattaaccg tcacgtcggc
    21361 aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca acgtcacgcc
    21421 atcggtagca ttcaccagcg tcccatcgcc gttaaacaat gaggcattaa gggcgacgga
    21481 agtatcctgc ccggtcagac gcaaaaccgt accatcgctg atatcgatag tgccgctgga
    21541 agtagcatcg atagactgaa tatcctgatc cgcgccagta acgaacgtcg ccccatcacc
    21601 gacccacagt gacgaagcat aaggaaagat atccgcaaca tcgcctttca gggtgccttc
    21661 ttcgacacgt actgccgact gcgttgtacc gctggcggtc agctccagcg tacccgcccc
    21721 cgttttagta aatgtgctac cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc
    21781 gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc gcatttcaat
    21841 atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa tctccatcac
    21901 gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat ctccaagctg
    21961 cgagttgtcg ctcacctcca gcataccgga ggcgatttgt gttgtgccca aataactatt
    22021 attgtttgcc agacttacct gcccgtcgcc cgcatcgacc accacgtaga cgccatcttt
    22081 cccgtcaccg ccgatatgca tatcgtttcc acctgcaaga tctaccacat cctgactgcc
    22141 gataaccgtt ccgctgctgc cgctgacttc aaagccatcg ttgtatgatg tgccactctg
    22201 ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg actgcggatc
    22261 gtcctggaga gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg caccatcatc
    22321 aacgactata tcgccggtta gcgccatcga ctgcgccccc gacagcacgt agctgccgtt
    22381 ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc cttgattaac
    22441 agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg tcaatgagtg
    22501 cacaaaggtt tgttgggtgg agccaacgtt tagctctgcc tgattctggt acttatcaat
    22561 actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg tatcgccgac
    22621 attcatcagg gagttgctgc gacccagggt aacttcaccg ttttcaatct gcatctcacc
    22681 agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt tggtgatcaa
    22741 cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccaa taaccagcgt
    22801 ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa agccacccgc
    22861 cgcagtggaa ggaccatctc catagcctgc tgcgctattg ttctcatcca ctaacacgcc
    22921 gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag ccgtcgcgct
    22981 attggtatat atcgccccgc cataaccttc ggcagtgtta tttgtaaagg cagtgttatt
    23041 tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa catcgattgc
    23101 cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat caaaaataac
    23161 atcacttaaa tagacgtcat tattaatggt ataaattgcg ccacctttgc catcattagc
    23221 gatattattg cgaaacatgg cgttagtgac acgtaaatcg acggcaccag tatcattagt
    23281 accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccggaaa aaataacatc
    23341 agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat tatattctcc
    23401 tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta atagtgaaaa
    23461 ttccgctcct tgttgcaaaa atacggctcc tccgctggcg ttattggtca tatccgaaaa
    23521 aaccagccat tgcccatcgg cgacactcca gtcctgcgta atgcctgaaa ggctttgcct
    23581 gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg ccgccgcgac
    23641 accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat actccttgcg
    23701 tagaaagata atccgcatat taatctgacc atcatttt
//
LOCUS       DAPPNJ010000062        23721 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000062 DAPPNJ010000000
VERSION     DAPPNJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.062"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..3860)
                     /gene="entF"
                     /locus_tag="RPM73_001970"
     CDS             complement(<1..3860)
                     /gene="entF"
                     /locus_tag="RPM73_001970"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDX6520335.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPI"
     gene            complement(3857..4075)
                     /gene="ybdZ"
                     /locus_tag="RPM73_001971"
     CDS             complement(3857..4075)
                     /gene="ybdZ"
                     /locus_tag="RPM73_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDX6520336.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4078..5280)
                     /gene="fes"
                     /locus_tag="RPM73_001972"
     CDS             complement(4078..5280)
                     /gene="fes"
                     /locus_tag="RPM73_001972"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDX6520337.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5523..7763
                     /gene="fepA"
                     /locus_tag="RPM73_001973"
     CDS             5523..7763
                     /gene="fepA"
                     /locus_tag="RPM73_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDX6520338.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7938..8558
                     /gene="entD"
                     /locus_tag="RPM73_001974"
     CDS             7938..8558
                     /gene="entD"
                     /locus_tag="RPM73_001974"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDX6520339.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8680..8832)
                     /gene="hokE"
                     /locus_tag="RPM73_001975"
     CDS             complement(8680..8832)
                     /gene="hokE"
                     /locus_tag="RPM73_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDX6520340.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9183..9335)
                     /gene="hokE"
                     /locus_tag="RPM73_001976"
     CDS             complement(9183..9335)
                     /gene="hokE"
                     /locus_tag="RPM73_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDX6520341.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            9777..10895
                     /locus_tag="RPM73_001977"
     CDS             9777..10895
                     /locus_tag="RPM73_001977"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDX6520342.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10961..11209
                     /locus_tag="RPM73_001978"
     CDS             10961..11209
                     /locus_tag="RPM73_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDX6520343.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11274..11642
                     /locus_tag="RPM73_001979"
     CDS             11274..11642
                     /locus_tag="RPM73_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDX6520344.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     KLRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11736..12389
                     /gene="nfsB"
                     /locus_tag="RPM73_001980"
     CDS             11736..12389
                     /gene="nfsB"
                     /locus_tag="RPM73_001980"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDX6520345.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12497..13744
                     /gene="ybdG"
                     /locus_tag="RPM73_001981"
     CDS             12497..13744
                     /gene="ybdG"
                     /locus_tag="RPM73_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDX6520346.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13812..15188)
                     /gene="pheP"
                     /locus_tag="RPM73_001982"
     CDS             complement(13812..15188)
                     /gene="pheP"
                     /locus_tag="RPM73_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDX6520347.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15290..18433)
                     /gene="cusA"
                     /locus_tag="RPM73_001983"
     CDS             complement(15290..18433)
                     /gene="cusA"
                     /locus_tag="RPM73_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDX6520348.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSTVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18445..19668)
                     /gene="cusB"
                     /locus_tag="RPM73_001984"
     CDS             complement(18445..19668)
                     /gene="cusB"
                     /locus_tag="RPM73_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDX6520349.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19684..20016)
                     /gene="cusF"
                     /locus_tag="RPM73_001985"
     CDS             complement(19684..20016)
                     /gene="cusF"
                     /locus_tag="RPM73_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDX6520350.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20040..21422)
                     /gene="cusC"
                     /locus_tag="RPM73_001986"
     CDS             complement(20040..21422)
                     /gene="cusC"
                     /locus_tag="RPM73_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDX6520351.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21579..22262
                     /gene="cusR"
                     /locus_tag="RPM73_001987"
     CDS             21579..22262
                     /gene="cusR"
                     /locus_tag="RPM73_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDX6520352.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22252..23700
                     /gene="cusS"
                     /locus_tag="RPM73_001988"
     CDS             22252..23700
                     /gene="cusS"
                     /locus_tag="RPM73_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDX6520353.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5706 a         6486 c         6037 g         5492 t
ORIGIN      
        1 ataatcggcc caatttttac aaacgcccct ggagagataa tatccacatg cgcacaatcc
       61 tgacgataga tatccagctc cgcaatccac ggcgaccagg cgcgttcggg gctcatacct
      121 tcctgaagcg tgcgttcagc aacaaacagc gtcgctttac catcaaacgg tacgctatga
      181 gcagtcgtca gcaggcgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc
      241 gttgaagtac ttccctgctg tgccgccagg aaggcctcgc gttcgcggtt aatctccgcc
      301 agcacttccg ggtccagacc attagcctct ttttcctgcc agttttgcgt ctctggcggc
      361 caggtatcca gcaaaccaag aaatgccacc tgttcgccac gggcacgcag ccgcgccgca
      421 atgccctgcg ccagcgtacc gccaagggaa taccccagca ggtaataagg gccgtgcggt
      481 tgttgctcaa gtaacgttgc cagatgcgct tcgcagactt catccaggtt tgtcgctgtc
      541 tgcatgggtc catgagggcg cggcgactga atgccaataa tcgaccattg tggatcgaga
      601 taacgcgaga gcacgctaaa ctgccaggca aaaccggacg ccggatggaa acaaaacagc
      661 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg ccgggagctg
      721 tcctcttcac catcaataat cgttgccagt ttggcgacgg ttgacgcgac catcacctgc
      781 cccggcgtca cctgacgagc aaactgtcga cttaactgcg ctgccagttt cattgccagt
      841 agcgaatgac cgcccagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc
      901 aacgacgcga atgccgcggc gataatcttt tcactgcccg ctttcggtgc acgtcccgac
      961 gcttgtgtct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
     1021 agaggtaact gtggaagttg cagcagaacc accggcacca tatgcggcgg caatgtttcg
     1081 cgaagctgtg cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag
     1141 cccaccaact gacgcgcatc accaccggtg gctgccgcca ggttaatcac acaggcgtgg
     1201 gtaacggctt gtttgacatc cggcagcgcc tgcatcacgc gatcgatttc gcccagttcg
     1261 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc caccgcgccg
     1321 ttatccagcc agcgggcgac gtctccggta cgatacatcc gttcacctgg ggcaaaagga
     1381 tcggcaataa aacggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttgg
     1441 atgccggtga gatagagatc acccgccaca cccggcggca ccggatgcat catcgcatca
     1501 agaatacgca gacccgtatt ccataccgga taaccaatcg gcacactgct gccgcgcacc
     1561 tgtgccagtt cctcgccaaa agccggatac cagctgacat ctaccgccgc ttccgtcggg
     1621 ccatatagat tatgcaacgg cgcgccagtt aactgttgcc attcgcggca taaatcagcc
     1681 ggtaaggcct caccactaca gaaaacctgt ttcaacgtcg cgcaactctg gcgagcggtt
     1741 tgcggcgtca gcgaggcaac aaatgccgcc agcatcgacg gcacaaagtg cgtggtcgtt
     1801 acgccatatt cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca
     1861 gccatcacca gtttcgcccc ggcaataaac ggccagaaaa actcccacac cgagacatca
     1921 aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcgggta gtgattttgc
     1981 atccataaca ggcggttaac gatagccgtc tgcccgacca ttaccccttt cggcctgccg
     2041 gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag ttgcagcggc
     2101 gcactgccct ggggggtaag cggggcgtta tagcaaaggc ttgttaaatt gggaacatcg
     2161 ctaaagcgcg gcagttgatc gtcggtggta attaacagcg acggacgcgc atcttccagc
     2221 atcattttca ggcgatcgtc cggatagccg gtatccagcg gtagccaggc cgcgccagct
     2281 tcaactattg catggagtgc cagggtcaaa aagaccgagc gcggtagtgc caccgccacg
     2341 ctgtcccctg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctcg
     2401 cgcatttccc gatagctgaa caggtaacgc gcatctgcca gcgccggagc atccggtgtt
     2461 tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc aacctgagtg
     2521 gcgttgatct gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac
     2581 aacagcgccg gatccgcggc gaactgggca atcagcattt tcaggcgttc agcatgctgg
     2641 attaacgttg gctcatcgta acgctgttta ttggcgagga tctcaatact caaatcaccg
     2701 tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc cagggtatgg
     2761 gtttgcgcct gaacaccagg aatatccagt tggtaatcaa ataccttaat attgagtacc
     2821 ggaccaaaca gcggttcatc acctgccgct cgcccgctgt cacggacaat ttgttcggca
     2881 tcgtaacgtt gatggcgacg catttttttc agttgagccg ccagtcgggt tgccagttcc
     2941 ggcagcgttt cttgcgccgc aatgtgaata cccaacggta aaacgttgag cacgggtccg
     3001 ctagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc ggcatagtcc
     3061 atgcgattgc acaatcgccc cagccacaag gctgccagcg caagggctaa atcggtacgc
     3121 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa ttccagtttc
     3181 aggcgcagaa tatctgccga agcgctgcgc cccgctaaag gtgccggaga aagtgacgcg
     3241 ggcggcggca gttgacgacg ctgttctgcc cagaatgccg catcacgctg ccaggcttcg
     3301 ctttcgcggt attgctggta ctcttccact acatcagcga aaggcgtaaa cggcgaagca
     3361 ggcgttggtt cgccacgcag ccatgtgcag taaatattgg cgatctggcg agtaatggcc
     3421 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
     3481 tccgcaacat gtatcagctg atgaaagacc agtggtttac cgctatcgac gcgcaaatct
     3541 tgttgcaaat ccgccagcat taatgcctgc gcagtaccgt gcggatcaat attggttcgc
     3601 aggtcgataa tttctggcag ttcgaatatc agcgcatcat cgacccactg ccagacttcg
     3661 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc gacaaccacc
     3721 gcgcgggcca gtaatggcgc atcaacatct ccagttaact caacgtaatg cgccacgctc
     3781 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
     3841 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattggcc ggtgtcagag
     3901 tacgccagtg gacttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
     3961 tgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
     4021 tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca
     4081 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
     4141 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttatggg
     4201 gtgtaattgc gcatacagcg cctgattggc ccgcataatc atcggctcgc gaatacccgc
     4261 ttccagcaca atgcgcagac cttcggcgct aacttcacca gctttaagct tttcaagtaa
     4321 cacgccctct tgctgcccgc cccgatgcgg ccaccagtac gatcctgact ggcttaatac
     4381 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagtc caccaaaact
     4441 ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcaccag
     4501 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgagc
     4561 gcggtgcgta gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa gttgctgacg
     4621 atgggtcaac gaagtcagca caggccagac aggcatactc tgcgcccaaa attcgccatc
     4681 gagcaaaact gccagcgggc gttctcctgc tgttgcatcg ccagtggtaa aaatccatac
     4741 acggcgtgaa tttttcaacc gttcactttt ccagataatt tctttggcag gcgtttctgg
     4801 cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac
     4861 agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta ttgcctgggg
     4921 taatagtttt cgccagcctt cacgtaattc gaggcgatcg ggggatggtg cagaaaaaat
     4981 gtcatcgcgt tcggtgggaa taaaacagta gctgccgcgc cagttggcat tgagttgtgt
     5041 cgtccactgc cagacgttgg tgcctgcaat tcgctgcatc gactggggct ggctgttctg
     5101 atggtgatcg gtcacaccag tgatgtagac ccatacgcgc tttatcgtcg agtattcttc
     5161 agaaccttgg ggatcacgcc accagaaagt gacctcaaac atttcgtcat tcagacgctg
     5221 ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac
     5281 accataaccc catgtttact gtgcaatttt tcattgattg cagaaatata ttgataatat
     5341 tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt
     5401 aattaaccaa ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg
     5461 tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa
     5521 caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt tatggggtag
     5581 cgcaggcaca agagccgacc gatactcctg tttcacatga cgatactatt gtcgttaccg
     5641 ccgccgagca gaacttgcag gcacctggcg tttcgaccat caccgcagat gaaatccgca
     5701 aaaacccagt tgcccgcgat gtgtcggaga tcatccgtac catgccaggc gttaacctga
     5761 ccggtaactc caccagtggt cagcgtggta ataaccgcca gattgatatt cgcggcatgg
     5821 gtccggaaaa cacgctgatt ttgattgacg gcaagccagt aagcagccgt aattcggtgc
     5881 gtcagggctg gcgtggtgag cgcgataccc gtggtgatac ctcctgggtg ccacctgaaa
     5941 tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc gcgttatggc aacggcgcgg
     6001 cgggcggcgt ggttaacatc attaccaaaa aaggcagtgg tgagtggcac ggctcctggg
     6061 atgcttattt caatgcgcca gaacataaag aggaaggtgc caccaaacgt accaacttca
     6121 gcctgaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac ctcgacaaaa
     6181 cccaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc ggaacgtatg
     6241 ccacgacgtt accagccggg cgcgaagggg tgatcaacaa agatattaat ggcgtggtgc
     6301 gctgggactt cgcgcctctg caatcactcg aactggaagc gggttacagc cgccagggta
     6361 acctgtatgc gggcgatacc cagaacacca actctgacgc ttacactcgc tcgaaatatg
     6421 gcgatgaaac caaccgcctg tatcgccaga actactcgct gacctggaac ggtggctggg
     6481 ataacggcgt gaccaccagc aactgggtgc agtacgaaca cacccgtaac tcgcgtattc
     6541 cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcgaca caggatttcg
     6601 tcgatatcga tcttgatgac gtgatgctgc acagcgaagt taacctgccg attgatttcc
     6661 tcgttaacca gacgctgacg ctgggtacgg agtggaatca gcaacggatg aaggacttga
     6721 gttccaatac ccaggcgctg accggggcga acaccggcgg cgctattgat ggtgtgagtg
     6781 ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc gaaaacaaca
     6841 tggagctgac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat cacagtattg
     6901 tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg tttaggcgat gacttcacgc
     6961 tcaaaatggg cattgcccgt gcttataaag caccgagcct gtaccagact aacccgaact
     7021 acattctcta cagtaaaggt cagggttgct atgccagcgc gggcggctgc tatctgcaag
     7081 gtaacgatga cctgaaagca gaaaccagca tcaacaaaga gattggtctg gagttcaaac
     7141 gcgacggctg gctggcgggc gtcacctggt tccgtaacga ttatcgcaat aagattgaag
     7201 cgggctatgt ggctgtagga caaaacgcag tcggcaccga tctctatcag tgggataacg
     7261 tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa cgtaccggtt agcgaaacgg
     7321 tgatgtggac caataacatc acttatatgc tgaagagtga aaacaaaacc acgggcgacc
     7381 gtttgtcgat catcccggag tatacgttga actcaacgct gagctggcag gcacgagaag
     7441 atttgtcgat gcaaacgacc ttcacctggt acggtaagca gcagccgaag aagtacaact
     7501 ataaaggtca gccagcggtt ggcccggaaa ccaaagaaat cagtccttac agcattgtgg
     7561 gtctgagcgc gacctgggat gtgacgaaga atgtcagtct gaccggcggc gtggacaacc
     7621 tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat ctggcagggg
     7681 ccaactatat cgcgggtgcc ggggcgtata cctataacga gccgggacgt acgtggtaca
     7741 tgagcgtaaa cactcacttc tgatgctaac gtcagattgt tgacaaagtg cgcgtcgttc
     7801 atgccggatg cggcgtgaac gccttatccg gcctacaaaa atcttgccaa ttcaatatat
     7861 tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc agtttagcgt ttatcatcag
     7921 tctcggatat ggtcgatatg aaaactacgc atacctccct cccctttgcc ggacatacgc
     7981 tgcattttgt tgagttcgat ccggcgaatt tttgtgagca ggatttactc tggctgccgc
     8041 actacgcaca attgcaacac gctggacgta aacgtaaaac agagcattta gccggacgga
     8101 tcgctgctgt ttatgcctta cgggaatatg gctataaatg tgtgcccgca atcggcgagc
     8161 tacgccaacc tgtctggcct gcggaggtat acggcagtat tagccactgt gggactacgg
     8221 cattagccgt ggtatctcgt caaccgattg gcattgatat agaagaaatt ttttctgtac
     8281 aaaccgcaag agaattgaca gacaacatta ttacaccagc agaacacgag cgactcgcgg
     8341 actgtggttt agccttttct ctggcgctga cactggcatt ttccgccaaa gagagcgcat
     8401 ttaaggcatc ggagagacaa actgaagcag gttttctgga ttatcagata attagctgga
     8461 ataaacagca ggtcatcatt catcgtgaga atgagatgtt tgctgtgcac tggcagataa
     8521 aagaaaaaat agtcataact ctgtgccaac acgattaatt gacaacatct ggtacgattc
     8581 gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac cactctgacc
     8641 tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc
     8701 gagaacagcc ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgagtctcc
     8761 gaccagaagc gtaaatccca gtaccgtcaa acacagcact atgactgcca caagggcata
     8821 tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc gagttgttga
     8881 ggctcggagt ggcagcccca ggattgatag aaatatctcc tggggcttca tctttccaga
     8941 cctcagagtt aacctgaaac cggaaagctt caggcacccg ccgttatctt acccttcctc
     9001 aacaaaatag ctgtttattt atacagtgaa tcctgtttcg gacttctgtc ggcaaaactc
     9061 atcattgctc ttaaaacgaa atcgcccgca gccatcactg accgcgggcg aaagtgtaaa
     9121 gcaggtgcct taccatcctg acctgacaac cggatatgcg gggattgctc cccgcacaac
     9181 ggctacttct tcggttcgta agcgagaaca accttaaact caatattacg ttcctttacg
     9241 gtgaactcac acagcgaatc cccgaccaga agcgtaaatc ccagcaccgt taaacacagc
     9301 actatgactg ccgcaagggc atatttcgtc agcatgttta tattgcctcc atgcgaagaa
     9361 gggactacca tccaaattgt tcaggtttgg gtaggtagcc ccagggttga tagaaatatc
     9421 gtcctggggc ttcatctttc cagacctcag agtaaagctg aaaccggaaa gcttcaggca
     9481 cccgcagtta tcttaccctt gcttagcaaa atagctgttt atttatacag taagtcctgt
     9541 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
     9601 tcaatcgtca aaattcttcg ccaccagcca gccgtgccat aacttgtcac attgcaaaag
     9661 tagccaaatt gcttcagtca ccatcaccaa aaaaagaaac attccagaac ttccgcatct
     9721 cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacttaagag aaacctatgc
     9781 cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg
     9841 tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta
     9901 aaaatgagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
     9961 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca gttttcagcg atgcagaaag
    10021 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acgcacccgt
    10081 ttcagaaatg gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga acgctggaaa
    10141 actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgcg
    10201 ccagtggcga tgacgccatt tatttgctgc acggcctgtc acggtttgtg ccgcacttca
    10261 tcgccctttc cgccgcgtcg ccgtatatgc agggaacgga tacgcgtttt gcctcctcac
    10321 gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc agtaactggc
    10381 aacaatttga agccctgttt cgctgcctga gttacaccac gatgatcgac agcattaaag
    10441 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    10501 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gccaccgccc
    10561 actggttact gacggaacgc ccgtttaaac atcaggagaa agattacctg ctgtataaat
    10621 tcaaccgttt ccaggcctgc cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    10681 gcgatcgtcg accgctaacg gaagatacct tgcgattgct ggaaaaaatc gccccttccg
    10741 cacataaaat gggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    10801 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    10861 tgaaaaagca ttgtgagatc tgggccggtg actaagtagg gaattgcctt acagaagaat
    10921 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    10981 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    11041 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    11101 tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    11161 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    11221 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    11281 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    11341 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    11401 cgtcgaagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    11461 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    11521 tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg atcaacgatt
    11581 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    11641 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    11701 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    11761 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    11821 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    11881 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    11941 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    12001 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    12061 ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    12121 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    12181 acttcctgct cggcgtggcg gcgctgggtc tggacgcggt acccatcgaa ggttttgacg
    12241 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    12301 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa tcacgtctgc
    12361 cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg cccggctatt
    12421 tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt
    12481 gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag cgggtattga
    12541 gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga ccgccgtcgt
    12601 ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg ccatcgccag
    12661 ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt tagcttttac
    12721 cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc
    12781 ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt
    12841 cttctcgttg ctggatgtga ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt
    12901 accgctgaaa gggatatttc aggggattaa actgatcggc gcgattctgg tcggcatttt
    12961 gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc ttggtgcaat
    13021 ggctgccgtg ctgatgttgg tttttaaaga tccgattctt ggtctggtgg caggtattca
    13081 gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga aatacggcgc
    13141 ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact gggacaatac
    13201 cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact ggagcgggat
    13261 gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca ccagtattcg
    13321 ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa agccttattt
    13381 aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta cggagtcgat
    13441 attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga acgaatatct
    13501 gcgtaaccat ccgcggattc gtaaagatat gactttaatg gtacgccaac tggctccagg
    13561 tgataacggt ttaccgctcg agatctatgc gtttaccaac accgtggtgt ggctggaata
    13621 tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag agtttggtct
    13681 gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg catttaagca
    13741 gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt gtcggatgta
    13801 gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa tacaatccac
    13861 accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag cagcaaaatc
    13921 atgccgagga aggcaatgca aagatagttg ccgaacggat agagcagcgc cttaaactgt
    13981 gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag gcagatcata
    14041 atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa cgctttttgc
    14101 ggcagcagat agttgattaa taccaccagc gaagtgatcg ctccggaaag catcagcgaa
    14161 ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt accctgcaca
    14221 gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac tgacagcgat
    14281 gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag attatggaaa
    14341 atcatcacaa acgggctact gttggatttc acttccaccc acggatagag cgccagtaaa
    14401 accaccagtg agccgatgta aaacagcagg atgcgataca ccacctgatt taccgctttt
    14461 ggaatgcttt tttccggatc gcgcgcttca gcggcagtaa tcccaatcag ctccagaccg
    14521 ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc ggtggcaaag
    14581 aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg accagaaaac
    14641 agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat cagcgcaaac
    14701 cagaactcgg tttcaccata taagcgcacg ttcaccaggt taacggcgtt gatgataata
    14761 aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg catatagatg
    14821 cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta gttccagcca
    14881 gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa tgaaccggat
    14941 accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc gatgatcccg
    15001 gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg gccaatgcca
    15061 agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg acggttatgt
    15121 aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac ggttgacgcg
    15181 tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta acatttagtg
    15241 cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt tatttccgta
    15301 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
    15361 aaggtgcggt gatcatgccg ccaatcatcg gcgcggcaat ccggctcatc acctctgaac
    15421 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
    15481 ctttcgggcg cacgcgcagt accgcgccgt gatacaacgc ctcatccagc ttctgctcac
    15541 tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt aaatacatca
    15601 gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaagcca gtacccgtcg
    15661 ccacagaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc agcgcaaacg
    15721 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga tacaacagca
    15781 cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg gcgcgctcca
    15841 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
    15901 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
    15961 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
    16021 taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggcaact
    16081 ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta atgggataac
    16141 gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgtagaa gtcacaaaca
    16201 actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta atctcaacgt
    16261 tgatatagcg cccaccttcc agacgctcgg caagcgcaga agccacacct ggtaccgttc
    16321 gtgccacctc ttcaatttgc tccgccatcg cgtcgatatc cgccagcaca gtgccggaaa
    16381 ctttaattcc gatggggctt ttaatgccag tcgagagcat atcgatacgg ttacgaattg
    16441 gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt tcctcaatga
    16501 ttttgtccat cgtcatgcct ggccgccact gctcctgcgg cttaagctgg atggtcgttt
    16561 ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt ttcccaaata
    16621 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
    16681 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
    16741 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
    16801 gcgccgccac cagcagcgtg gttttcggcc agtgcagtac tttcagcaac agcggatgat
    16861 aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt ttgccacgga
    16921 tccagtagcc catcaggatc gggatcacta cgatcgccag cagcgccgca cccgccatcg
    16981 catacgtttt ggtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
    17041 agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc ggccccactt
    17101 caacagacgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
    17161 gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg atagcggcat
    17221 cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg gcattcagtc
    17281 cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
    17341 ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc acaataaact
    17401 cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg ctgcgatcgt
    17461 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
    17521 tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc accccgcccg
    17581 ccacttcacc ttcgccattc agttcggcaa tgccccggcg catctccggg ccaatctgga
    17641 ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt aaaacgatgt
    17701 gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat tccgcttccg
    17761 ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt
    17821 cggctagact gatgccatat tgcgccaggc gctggggatc gataaccacc tgatactctt
    17881 tcaccacacc gcccaccgac gccacttccg caacgtcagg gatggttttc agctcatatt
    17941 tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg ctgcgatcca
    18001 ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc tcggcgctga
    18061 ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc
    18121 agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca aactgtgaga
    18181 aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc ggataagtca
    18241 cctgattttc aacgatttgt ggtgcctgac cgggatagct ggttttaata ataacctgca
    18301 catcggagag atccggcagc gcatccactg gcgtattaat gatggtccag gtgccccaga
    18361 tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa
    18421 tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag agcgcatccg
    18481 ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac
    18541 cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg cctggaaaac
    18601 agcaacgcgt ttcggtacaa agcgtccgtc ggcatcaacg gtaatcaccc gctgttcgtt
    18661 gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg cggtattgag
    18721 ttgcaaccag gcgttcattc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg
    18781 cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt tgcggatggt
    18841 gagtgtttta tcaggtcgcg ccggaacggt gagcgtaaac tgcgaggcat ctttcaccag
    18901 ccaggcgatg gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt
    18961 cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
    19021 gccatcaatg ggcgctttga gcgtaaaacg agtctggatt ttttgcgtgg cgatcaggcg
    19081 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
    19141 ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc
    19201 aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
    19261 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
    19321 gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggcccgcgcg tgacggtagc
    19381 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
    19441 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
    19501 atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac gttctgcggt
    19561 cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg ccgcagaaat
    19621 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
    19681 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
    19741 aaacgccact ttgtcgccag ttttaatttc actcattttc gtctgcggag tgatggtaaa
    19801 gcgcatggtc atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt
    19861 tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct gcggttgtac
    19921 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
    19981 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
    20041 tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac ctgacgggca
    20101 taattcaggt caagtaaagt ttgtcgggtt gcaaataaag agcgctcggc atccagcact
    20161 tccagataac ttaccgcgcc gtgctgatat aatgcccgtg cccgttgcag ggtaatttgc
    20221 agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg acgtaatgcc
    20281 agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac
    20341 tgctgctggc gaatttcggc gatatccaga ttggcttggt tgcgtccggc attaaaaatg
    20401 gggatctcaa ttttaggaat aaaattccac atcccgctgc tggcgttaaa taatgaagac
    20461 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
    20521 cgcgcagcgc caatattggc attagccgcc attaacgcgt gttcagcttc cataatatcc
    20581 gggcgctgca ataagatttg cgacgacaat cccgccggta atttaacgct ttgcaggctg
    20641 tcgctgttta ctgtctgcgc ttgcggcagc ttgccgtagc ttcccaataa cagttgcaat
    20701 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcgctgcg ggtactttca
    20761 atcaccccgc gagcctgttc cagcgccaga acattactgc tacctgtcaa cagttgtttt
    20821 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
    20881 gcatacgcta gctgctgatt gaaataactt tgcgcgacat tagaaaccag caaaatatgc
    20941 accgcgcgct gggcttcctc agtggctaaa taattttggc gctcggcttc gctcatgttc
    21001 tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga aaactcccgt
    21061 gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc gccattgagc
    21121 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
    21181 gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt cttcacctga
    21241 ttatcaacaa aaaaggtgcg ccagcccgcg ttctgatagt tatctgctgc gttaacgagg
    21301 ccgttctggc tgagtgagaa ctgctgcggt acgggcattg ccgggcgctg ataatccggt
    21361 gccagtgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
    21421 ataggctcat aatttctgat gattttatgc cgccaacttt actcgccagg ctctgatttt
    21481 ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
    21541 gctactctag aattgcccgg gcaacatgcg gaggaaatat gaaactgttg attgtcgaag
    21601 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
    21661 atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat tatgatctga
    21721 taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttacgct
    21781 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
    21841 tcaaggggct ggagttgggg gcagatgact acctgatgaa accattcgct tttgctgaac
    21901 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtaattatc gaaagtcagt
    21961 ttcaggttgc cgacctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
    22021 gcatcacttt gaccagtaaa gagtttactc tgctggagtt ttttctccgc catcagggcg
    22081 aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc gacagcgaca
    22141 ctaacgccat tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac gactttgagc
    22201 cgaagctgat tcagaccgtg cgcggcgtgg gctatatgct tgaggtgccg gatggtcagt
    22261 aagccatttc agcgcccgtt ttcgctggca acccgtctga ccttttttat cagcctggcc
    22321 accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcagtgaa agtgcatttt
    22381 gccgagcagg atattaatga tttaaaagaa attagcgcca cccttgaacg ggtacttaat
    22441 catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
    22501 tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca ctcccccggt
    22561 gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc gcggggcggt
    22621 gaggtgtatc tcctttccgg cccgacgatg atgatgccag gccacggtca cgggcatatg
    22681 gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga cggcaaaccg
    22741 atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg
    22801 atgaataaac ttattatgac cgcatctgtc atcagcatcc tgatcgtctt tatcgtgcta
    22861 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc
    22921 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg tgcccattga gctggaacag
    22981 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc
    23041 aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct catcacgcaa
    23101 acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt gctctactct
    23161 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
    23221 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
    23281 aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt gcggtttgtt
    23341 ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg
    23401 ctttctaatg ccctgcgtta tacgccaccc agtgaggcta ttgtagtgcg ctgccagacg
    23461 gtcaatcatc aggtgcaagt ttccgtcgaa aaccccggta cgcccattgc gcccgagcac
    23521 ttaccgcgat tgtttgaccg tttctatcgc gttgaccctt cccgccagcg aaaaggtgaa
    23581 ggtagcggta ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
    23641 gcggtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga gagagagtga
    23701 ttattaagtt caaattcagg g
//
LOCUS       DAPPNJ010000063        23619 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000063 DAPPNJ010000000
VERSION     DAPPNJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.063"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            14..1642
                     /locus_tag="RPM73_001989"
     CDS             14..1642
                     /locus_tag="RPM73_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDX6520354.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1881..1940
                     /locus_tag="RPM73_001990"
     CDS             1881..1940
                     /locus_tag="RPM73_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX6520355.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2257..2316
                     /gene="ibsE"
                     /locus_tag="RPM73_001991"
     CDS             2257..2316
                     /gene="ibsE"
                     /locus_tag="RPM73_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDX6520356.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2436..3869)
                     /gene="hldE"
                     /locus_tag="RPM73_001992"
     CDS             complement(2436..3869)
                     /gene="hldE"
                     /locus_tag="RPM73_001992"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDX6520357.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3917..6757)
                     /gene="glnE"
                     /locus_tag="RPM73_001993"
     CDS             complement(3917..6757)
                     /gene="glnE"
                     /locus_tag="RPM73_001993"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDX6520358.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6780..8081)
                     /gene="ygiF"
                     /locus_tag="RPM73_001994"
     CDS             complement(6780..8081)
                     /gene="ygiF"
                     /locus_tag="RPM73_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDX6520359.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8323..8943
                     /locus_tag="RPM73_001995"
     CDS             8323..8943
                     /locus_tag="RPM73_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDX6520360.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            9007..10245
                     /gene="cca"
                     /locus_tag="RPM73_001996"
     CDS             9007..10245
                     /gene="cca"
                     /locus_tag="RPM73_001996"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDX6520361.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10408..11229)
                     /gene="bacA"
                     /locus_tag="RPM73_001997"
     CDS             complement(10408..11229)
                     /gene="bacA"
                     /locus_tag="RPM73_001997"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDX6520362.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11320..11688)
                     /gene="folB"
                     /locus_tag="RPM73_001998"
     CDS             complement(11320..11688)
                     /gene="folB"
                     /locus_tag="RPM73_001998"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDX6520363.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11793..12410
                     /gene="plsY"
                     /locus_tag="RPM73_001999"
     CDS             11793..12410
                     /gene="plsY"
                     /locus_tag="RPM73_001999"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDX6520364.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12423..13355)
                     /gene="ttdR"
                     /locus_tag="RPM73_002000"
                     /gene_synonym="dan"
     CDS             complement(12423..13355)
                     /gene="ttdR"
                     /locus_tag="RPM73_002000"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDX6520365.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13562..14473
                     /gene="ttdA"
                     /locus_tag="RPM73_002001"
     CDS             13562..14473
                     /gene="ttdA"
                     /locus_tag="RPM73_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDX6520366.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14470..15075
                     /gene="ttdB"
                     /locus_tag="RPM73_002002"
     CDS             14470..15075
                     /gene="ttdB"
                     /locus_tag="RPM73_002002"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDX6520367.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15123..16586
                     /gene="ttdT"
                     /locus_tag="RPM73_002003"
     CDS             15123..16586
                     /gene="ttdT"
                     /locus_tag="RPM73_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDX6520368.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16629..17642)
                     /gene="tsaD"
                     /locus_tag="RPM73_002004"
     CDS             complement(16629..17642)
                     /gene="tsaD"
                     /locus_tag="RPM73_002004"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDX6520369.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17880..18095
                     /gene="rpsU"
                     /locus_tag="RPM73_002005"
     CDS             17880..18095
                     /gene="rpsU"
                     /locus_tag="RPM73_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDX6520370.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18206..19951
                     /gene="dnaG"
                     /locus_tag="RPM73_002006"
     CDS             18206..19951
                     /gene="dnaG"
                     /locus_tag="RPM73_002006"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDX6520371.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20146..21987
                     /gene="rpoD"
                     /locus_tag="RPM73_002007"
     CDS             20146..21987
                     /gene="rpoD"
                     /locus_tag="RPM73_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDX6520372.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22066..22572)
                     /gene="mug"
                     /locus_tag="RPM73_002008"
     CDS             complement(22066..22572)
                     /gene="mug"
                     /locus_tag="RPM73_002008"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDX6520373.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22697..22772
                     /locus_tag="RPM73_002009"
     tRNA            22697..22772
                     /locus_tag="RPM73_002009"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22730..22732,aa:Ile,seq:cat)
     gene            complement(22826..23590)
                     /gene="nfeF"
                     /locus_tag="RPM73_002010"
     CDS             complement(22826..23590)
                     /gene="nfeF"
                     /locus_tag="RPM73_002010"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDX6520374.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5685 a         6509 c         6074 g         5351 t
ORIGIN      
        1 tgtgccctcc tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat
       61 tattttcgcc aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt
      121 aggtgggcaa aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat
      181 aatccccatc aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct
      241 gattacgaaa gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc
      301 gtctgtagaa ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga
      361 agacttacgt atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca
      421 aatgaccatg catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac
      481 tgtcgcagaa gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt
      541 taaccagacc tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac
      601 caaactgact caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt
      661 agaagttgcg gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca
      721 acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
      781 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
      841 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
      901 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacaacagc aggtcaccga
      961 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
     1021 agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac
     1081 cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
     1141 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
     1201 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
     1261 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
     1321 cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt
     1381 tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
     1441 cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc
     1501 attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga
     1561 aattggcgtt tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc
     1621 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg
     1681 gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg
     1741 cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct
     1801 catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg
     1861 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg
     1921 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     1981 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     2041 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
     2101 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
     2161 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     2221 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     2281 gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat
     2341 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc
     2401 ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     2461 ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg
     2521 ccgttggccc agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc
     2581 accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac
     2641 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     2701 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     2761 agacggtcac ccagtttgcg agcatttgcc agataggaga cgtgcccggc gtgcaggatg
     2821 tcaaagacac cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacagca
     2881 agcttcagtt cctcttcggt catcacacca aagccggtat ctgcgcgccc acgcactgca
     2941 ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc
     3001 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     3061 aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt
     3121 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     3181 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     3241 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc
     3301 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc
     3361 gctttacgcg ccagttggat catctgctgt acgcttgcca acgcaccttt ggcgtagtca
     3421 gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc
     3481 ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg gttgcgggaa
     3541 agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg
     3601 ttgacgtcgg ccagagattt actcagcgca cgcgccgcat cgtcaatgcc cgtcaacccg
     3661 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
     3721 ggacgttctt cgatggtatt gactttaacc acgggcaccg gcgcttccgg cgagatacga
     3781 ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     3841 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     3901 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat
     3961 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
     4021 gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt
     4081 ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta
     4141 acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg
     4201 taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag
     4261 cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca
     4321 gcgtcataat ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca
     4381 ctacacgcgc acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat
     4441 cagcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg
     4501 catccacttc atacaaaatg ccggaagagg ttcgcgtact gaacagatgc ataatgcgtt
     4561 gcgccagacg cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat
     4621 ccattgggca atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc
     4681 cgcccagctt gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat
     4741 ttggcttacc gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca
     4801 tagcctctgc cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga
     4861 tatccgctgc ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
     4921 gttgctcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg
     4981 catcggtcgc cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg
     5041 gataacgcgc cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt
     5101 taagcgccgc acggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca
     5161 ccagcaaggc ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac
     5221 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct
     5281 tatccagctc tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc
     5341 taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac
     5401 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
     5461 taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt
     5521 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt
     5581 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg
     5641 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
     5701 gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga
     5761 acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg
     5821 tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa
     5881 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt
     5941 tagcatagac gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc
     6001 agtcgcgccc ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc
     6061 cactttcgcc aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg
     6121 gttgatccaa cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
     6181 gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat
     6241 ctgaggagaa attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt
     6301 gcgcttcgcc ctgcgcattg cacggcgttc cccattcgcg gcagcaagcg tcatacagcc
     6361 agtcacgcgc ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct
     6421 cttcagtaac caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga
     6481 atagccgcag ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca
     6541 accatgccgc gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca
     6601 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
     6661 tcgcctgtgc gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct
     6721 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
     6781 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
     6841 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
     6901 caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca
     6961 cgcgtcagac gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt
     7021 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc
     7081 attttactct gcgctttggc atctaaaaac ggctgccagg ctttgcttac caaccattcg
     7141 gtcaacgcca gctttgccat tgccgtttcg gtagagtaga ccgccgtcac ggcagaaacc
     7201 gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta
     7261 cgcggcacaa tgccaccgaa cagcatcagt gcatggcgaa caaggccaat ggctgccagc
     7321 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
     7381 gctaacgcca gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca
     7441 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
     7501 cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
     7561 agtttcagca ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt
     7621 tcagcaaatt cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca
     7681 tcgaccgcca ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg
     7741 gaagcgagat cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg
     7801 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
     7861 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag
     7921 cccatatcgt gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagtaactgc
     7981 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca
     8041 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta
     8101 tgtcacataa gtgacgatga acggcgaact taatacgatc ttttttgtca gtagatagta
     8161 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
     8221 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
     8281 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
     8341 atcggattaa ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc
     8401 tatgtttctg atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
     8461 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
     8521 tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc
     8581 actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc
     8641 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
     8701 gtggcgcaga gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac
     8761 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagttgga tgacaaacag
     8821 cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
     8881 ctcggtctgg tactgccaca cctgatccca acgcgcaaac gcaaagatcg ctggatgaac
     8941 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
     9001 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg
     9061 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
     9121 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
     9181 gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc
     9241 gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
     9301 gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
     9361 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg
     9421 gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg
     9481 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
     9541 aaagagacgg aaagcgccct caccacccgc aatccacagg tgttcttcca ggtactgcgc
     9601 gattgtggcg cactgcgcgt tttattcccg gaaattgacg cactgtttgg cgttccggcc
     9661 cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
     9721 gcggcgatgc tgagtccgca ggtcgatgtc cgtttcgcaa ctttatgcca cgatctcggt
     9781 aaaggactga cgccgccaga actttggccg cgtcatcatg gtcacggtcc ggcgggtgtg
     9841 aaattagtgg aacaactctg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
     9901 agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaact
     9961 atcgtcaaat tgtttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg
    10021 gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg
    10081 caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc
    10141 gttgaagcgg gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg
    10201 gcggtagcca gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg
    10261 ttaacacgaa ccatcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca
    10321 gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac
    10381 caggcctgct cccgaacctt ttctcaatca aaagaacacg acgtacaccg cagccgccac
    10441 aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt
    10501 tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat
    10561 atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat
    10621 catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag
    10681 catcccacct gaaatggtgg ccccggaacg ggagaaaccc ggccacagcg ccagacactg
    10741 gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg
    10801 ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagtgcata
    10861 catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaacaaca gccccagtac
    10921 caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttctcc
    10981 ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa
    11041 catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt
    11101 gtcgccctcg aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg
    11161 cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat
    11221 atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgga ctactgcagt
    11281 aagaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt ctttcagatt
    11341 attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc ctggcttgct
    11401 gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg ccacctcttc
    11461 agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca ccgtttctgc
    11521 aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt tacggttatc
    11581 ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc agtcgtaaac
    11641 accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca cgtcctgcct
    11701 gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    11761 gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgcct ggaatgatcc
    11821 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ttgtgtgggc
    11881 tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg ttacgtatcg
    11941 gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcccg
    12001 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    12061 cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa ggcgttgcta
    12121 ccgcttttgg tgccatcgca cccattggct gggatctcac cggagtaatg gcgggaacct
    12181 ggttactgac tgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    12241 ttgctccatt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    12301 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    12361 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    12421 atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca ccatgccgcc
    12481 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    12541 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    12601 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    12661 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12721 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12781 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12841 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12901 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12961 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    13021 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    13081 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    13141 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    13201 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtc
    13261 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    13321 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    13381 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    13441 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    13501 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    13561 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    13621 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    13681 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13741 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13801 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13861 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13921 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13981 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    14041 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtagtgaaat tcgtcttcga
    14101 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    14161 cacctcggtg gaaaccgccg ccgtgctctc gcgaaaagcc attttgcgcc cgattggcag
    14221 ccgccatccc aatccaaaag cggcagagct ggagctacgc ctggaagaag gactcaaccg
    14281 tctgggaatt ggcccacaag ggctgactgg caacagttca gtgatgggcg tgcatatcga
    14341 atctgccgcc cgccatccgt caaccatcgg cgttgctgtt tctacaggct gctgggcgca
    14401 tcgtcgcggc acactgctgg ttcatgccga tctcaccttt gaaaatctgt ctcacacccg
    14461 gagcgcgtta tgaaaaagat cctgacaacc ccgatcaaag ctgaagatct gcaagatatt
    14521 cgcgtcggcg atgtgatcta cctgaccggt acgctggtga cctgccgtga cgtttgtcac
    14581 cgccgcttga tcgaactgaa acgtccgatc ccttacgatc tcaacggcaa agcgattttc
    14641 cacgctggcc ccatcgtgcg caaaaacggc gataaatggg agatggtttc cgttggcccg
    14701 acaaccagta tgcgtatgga aagttttgaa cgtgaattta tcgagcagac cggcgtgaaa
    14761 ctggtggttg gcaaaggtgg tatggggccg ctgaccgaag aaggctgcca gaaattcaag
    14821 gcgctacatg tgattttccc ggcaggctgc gcggtgctgg cggcaaccca ggtagaagag
    14881 attgaagaag tgcactggac agaactcggg atgccggagt cactgtgggt ctgccgggtc
    14941 aaagagttcg gcccgctgat tgtctctatt gatacccacg gcaacaacct gatagccgaa
    15001 aacaaaaagc tgttcgccga acgccgcgat cccatcgtgg aagagatctg cgagcacgtc
    15061 cattacatca aataaccctc ccggagaggc tcacccctct cctttttcgc aggcataaca
    15121 cgatgaaacc ttccactgaa tggtggcgat atcttgcgcc gctggcggtc atcgccatta
    15181 ttgctctact tccggttccc gcagggctgg agaaccatac ctggctctac tttgccgttt
    15241 ttactggcgt gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg
    15301 tgggtatctc catcatcgcc atactctctc cctggctgct gttcagcccc gaacagctcg
    15361 cccagccagg ctttaaattc actgcaaaat ccctctcgtg ggcggtttcc ggtttttcta
    15421 attcggttat ctggctgatt ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg
    15481 ggcttggacg ccgcattgcg ctgtttctgg tgaaaaagat gggacatcgc acgctgtttc
    15541 tcggctatgc ggtgatgttc tccgagctga tcctcgcacc tgtaacaccg tccaactcgg
    15601 cgcgtggtgc ggggattatc tatcccatca tccgtaacct gccaccgctc tatcaatcac
    15661 aaccaaacga cagcagttcg cgcagcattg gctcgtacat catgtggatg gggattgttg
    15721 ccgactgcgt gaccagcgcc attttcttga cggcgatggc accaaacttg ctgttaattg
    15781 gactgatgaa aagcgcatct cacgccacgc tgagttgggg cgactggttc ctcgggatgt
    15841 tgccgctcag tattttgctg gtgctgctgg ttccctggct ggcttatgtg ctgtacccgc
    15901 cggtactgaa gtctggcgat caggtgccgc gctgggcaga gacggaactg caggcaatgg
    15961 gcccgctctg ttcgcgtgaa aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt
    16021 ggattttcgg cggtgattat atcgatgccg cgatggtcgg ttacagcgtg gtggcactga
    16081 tgctgcttct gcgcattatc agttgggacg acattgtcag taataaagcg gcgtggaacg
    16141 ttttcttctg gctggcctcg cttatcaccc tcgctaccgg actcaacaac actggtttta
    16201 ttagctggtt tggcaaactg ttagcaggca gcttaagcgg ttattcgcca acgatggtga
    16261 tggtggcgtt gattgtggtg ttttatctgc tgcgctactt tttcgccagc gccacggcgt
    16321 atacctccgc cctcgcaccg atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc
    16381 tgccggtgtt ctgcctgatg gttggcgcgg caattggtct ggggagcatt cttacaccat
    16441 acgccaccgg acccagtccg atttactacg gtagtggtta tctgccaacg gtggattact
    16501 ggcgactggg ggcgattttt ggactgatat tcctcgtgtt gctggtgatt accggcttgc
    16561 tgtggatgcc cgtggtgttg ctttaagtga ggacggtgcc ggaaattgct ctccggcacc
    16621 ggatagtttt acgcagccgg taactctgcc agtggccagc gcggacgcac gctaacgccg
    16681 agatccgccg tcgcgcctgc tttaaaccgc accattccgg cataggcgat catcgcgccg
    16741 ttatcagtac aaaattccgg acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt
    16801 tccgccagct tcgcccgtaa cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc
    16861 ttaaagcccg tctgatccaa cgctcgctta cacttaatca tcaacgtatc gaccaccgca
    16921 tcttcaaagg cgcgggcgat atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc
    16981 gtgttcgccg caaaggtttt cagaccagaa aagctgaaat ccagccccgg acggtcggtc
    17041 atcggacgcg ggaaaacaaa gcgcccggca gtaccctgag ccgccatttt cgacagtaac
    17101 ggtccgccag gataatccag ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg
    17161 gcatcatcga tagactcgcc gagcagctcg tactgaccaa tgccagtcac gctgattaac
    17221 tgcgtatgac cgccggacac cagcagcgcg acaaacggaa attccggcgg gttatcttcc
    17281 agcatcggcg ctaacagatg cccttccata tggtgtaccg ggattgccgg aacgttccag
    17341 gcaaacgcca gagaacgccc cacggttgcg ccaaccagca gtgcgccgac taatccaggg
    17401 cctgcggtat aggccacagc atcaatgtct tttgccgtta aaccagactc ctttagcgcc
    17461 gcctggatca acggtacggt tttgcgcacg tgatcgcggg aggccagttc aggcacgacg
    17521 ccgccgtagt cagcgtgcaa tttcacctga ctatacaatt ggttggctaa caaacctttt
    17581 tcatcgtcgt aaatggcgat gccggtttca tcgcaggaag tttcaatacc cagtacacgc
    17641 atgacttgtt ttacctcgct ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa
    17701 aactttgttc gcccctggag aaagcctcgt gtatactcct cacccttata aaagtccctt
    17761 tcaaaaaagg ccgcggtgct ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt
    17821 ttgaaataag ctggcgttga tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca
    17881 tgccggtaat taaagtacgt gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc
    17941 gttcctgcga aaaagcaggt gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac
    18001 cgactaccga acgtaagcgc gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg
    18061 ctcgcgaaaa cgcacgccgc actcgtctgt actaattccc cgagagcgtt gctctccgat
    18121 cagaccgagt tgtagttgta aggccgtgct tccgaaagga atgcgcggct tattttcgtt
    18181 tatgaattgc taaaaatcgg ggcctatggc tggacgaatc ccacgcgtat tcattaatga
    18241 tctgctggca cgcactgaca tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca
    18301 gggcaagaat ttccacgcgt gttgtccatt ccacaacgag aaaaccccgt cattcaccgt
    18361 taacggtgag aaacagtttt accactgctt tggatgtggc gcgcacggca acgcgatcga
    18421 cttcctgatg aactacgaca agctcgagtt cgttgaaacg gtcgaagagc tggcagcaat
    18481 gcacaatctt gaagtgccat ttgaagcagg cagcggcccc agccagatcg agcgccatca
    18541 gaggcaaacg ctttatcagt tgatggacgg tctgaatacg ttttaccaac aatctttaca
    18601 acaacctgtt gccacgtctg cgcgccagta tctggaaaaa cgcggattaa gccacgaggt
    18661 tatcgctcgc tttgcgattg gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt
    18721 tggcggcaat ccagaaaatc gccagtcatt gattgatgcg gggatgttgg tcactaacga
    18781 tcagggacgc agttacgatc gtttccgcga gcgggtgatg ttccccattc gcgataaacg
    18841 cggtcgggtg attggttttg gcgggcgcgt gctgggcaac gataccccca aatacctgaa
    18901 ctcgccggaa acagacattt tccataaagg ccgccagctt tacggtcttt atgaagcgca
    18961 gcaggataac gctgaaccca atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc
    19021 gctggcgcaa tacggcatta attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga
    19081 tcacatacaa ctgttgttcc gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg
    19141 tgcaggccgc gatgccgcat ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg
    19201 ccgtcagcta cgctttatgt ttttgccgga tggcgaagac cctgacacgc tggtacgaaa
    19261 agaaggtaaa gaagcgtttg aagcgcggat ggagcaggcg atgccactct ccgcatttct
    19321 gtttaacagc ctgatgccgc aagttgatct gagtacccct gacgggcgcg cacgtttgag
    19381 tacgctggca ctgccattga tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg
    19441 tcaggaatta ggcaacaaat taggcatact tgatgacagc cagcttgaac gattaatgcc
    19501 aaaagcggca gagagcggcg tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg
    19561 tatacttata gggttgctgg tgcaaaatcc agaattagcg acgttggtcc cgccgcttga
    19621 gaatctggat gaaaataagc tccctggact tggcttattc agagaactgg tcaacacttg
    19681 tctctcccag ccaggtctga ccaccgggca acttttagag cactatcgtg gtacaaataa
    19741 tgctgccacc cttgaaaaac tgtcgatgtg ggacgatata gcagataaga atattgctga
    19801 gcaaaccttc accgactcac tcaaccatat gtttgattcg ctgcttgaac tgcgccagga
    19861 agagttaatc gctcgtgagc gcacgcatgg tttaagcaac gaagaacgcc tggagctctg
    19921 gacattaaac caggaactgg cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc
    19981 gggaagcccc cgacagccgc actgagaggc agcggcaaat atataagtac gccctcgtaa
    20041 ttatcgttgg cggtaaacaa ccgttggatt tcagcgttaa cggctgaagg acatcgggtc
    20101 aatcgcccaa caccaacctc atgaaataag tgtggatacc gtcttatgga gcaaaacccg
    20161 cagtcacagc tgaaacttct tgtcacccgt ggtaaggagc aaggctatct gacctatgcc
    20221 gaggtcaatg accatctgcc ggaagatatc gtcgattcag atcagatcga agacatcatc
    20281 caaatgatca acgacatggg cattcaggtg atggaagaag caccggatgc cgatgatctg
    20341 atgctggctg aaaacaccgc ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc
    20401 agcgtggaat ctgaaatcgg gcgcacgact gacccggtac gcatgtacat gcgtgaaatg
    20461 ggcaccgttg aactgttgac ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac
    20521 gggatcaacc aggttcaatg ctccgttgct gaatatccgg aagcgatcac ctatctgctg
    20581 gaacagtacg atcgtgttga agcagaagaa gcgcgtctgt ccgatctgat caccggcttt
    20641 gttgacccga acgcagaaga agatctggca cctaccgcca ctcacgtcgg ttctgagctt
    20701 tcccaggaag atctggacga tgacgaagat gaagacgaag aagatggcga tgacgacagc
    20761 gccgatgatg acaacagcat cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc
    20821 gctcagtacg ttgtaacgcg tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct
    20881 caggaagaga tcctgaaact gtctgaagta ttcaaacagt tccgcctggt gccgaagcag
    20941 tttgactacc tggtcaacag catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt
    21001 ctgatcatga agctctgcgt tgagcagtgc aaaatgccga agaaaaactt cattaccctg
    21061 tttaccggca acgaaaccag cgatacctgg ttcaacgcgg caattgcgat gaacaagccg
    21121 tggtcggaaa aactgcacga tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag
    21181 cagattgaag aagaaaccgg cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg
    21241 tccatcggtg aagcgaaagc ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt
    21301 ctggttattt ctatcgccaa gaaatacacc aaccgtggct tgcagttcct tgacctgatt
    21361 caggaaggca acatcggtct gatgaaagcg gttgataaat tcgaataccg ccgtggttac
    21421 aagttctcca cctacgcaac ctggtggatc cgtcaggcga tcacccgttc tatcgcggat
    21481 caggcgcgca ccatccgtat tccggtgcat atgattgaga ctatcaacaa actcaaccgt
    21541 atttctcgcc agatgctgca agagatgggc cgtgaaccga cgccggaaga actggctgaa
    21601 cgtatgctga tgccggaaga caagatccgc aaagtgctga agatcgccaa agagccaatc
    21661 tccatggaaa cgccgatcgg tgatgatgaa gattcgcatc tgggggattt catcgaggat
    21721 accaccctcg agctgccgct ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac
    21781 gacgtgctgg ctggcctgac cgcgcgtgaa gcgaaagttc tgcgtatgcg tttcggtatc
    21841 gatatgaaca ccgaccacac gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa
    21901 cgtatccgtc agatcgaagc gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa
    21961 gtgttgcgta gcttcctgga cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc
    22021 ggcactagtc cctttgcaca aacgccacct tttcggtggc gttttttatc gcccacgcac
    22081 taccagcgcc tggtccagct cgcgatacgc ttcaaccagt ttctctagtg aaacgcgact
    22141 taaaccgctg ggatttggca gcacccaaat ctgcgtcgaa ccaatggtga gcgtttgttt
    22201 cccccactgt gcaccgcgct ggctgaatcc ctgttcatat gcttgtttgc ccagaatcgc
    22261 caacgcctgc ggctgataat cttcaatttt ttcaatcagc ttacgcccac ctgcgtgcag
    22321 ctcctgcttt gaaacttcat tggcttgcac cgttggacgg tctaccagtt tggtgacgcc
    22381 acaacgataa tccagcagat gctgtgcctc ctgcggcttc aactgacggt cggtaaaccc
    22441 ggcctgatat atcaccttcc agaagcgatt tgctggatga gcaaagggaa aaccagtccc
    22501 ggcggatgaa agcccagggt tgataccgca aaacacgacc cgtaaccctg gagccaaaat
    22561 atcctcaacc atatttactc ctttctaaca tcaccaggaa aagtataacg gattgaaaat
    22621 acattgttta taaaaacagc aggcgcacgg taatggctgg attgcgacac ggagttactt
    22681 tataatccgc taccatggcc ccttagctca gtggttagag caggcgactc ataatcgctt
    22741 ggtcgctggt tcaagtccag caggggccac cagatatagc aaaggctgac gagaaatcgt
    22801 cagccttttt ctttttatat atcagttact ttgcgtgcca gtaagccgct gcacgtaccc
    22861 gctgtgggtc atactgttcc gcttcaaagc ggcggcttaa attcttaacg actttacctt
    22921 cgccggttat ccagatgaag taatcatcgg cagggatttg catctgcgcc agacgcgcat
    22981 ctaccgcctg ctcatcatgt gccagccatt cgatattaaa accatcaagg tgcgcgagat
    23041 aatcctgaca ggcgttatcc cgcacgctaa ccagcgcact aacttgcggt ttaacggcaa
    23101 gtttgctcaa cgtttccagg cggcggcgca gtgcgggcat tccggattca tcgcagacat
    23161 acagctgata cgcgtaatct tccggcacca ccagcgaacc gcgcggacct gccaccgtaa
    23221 gtttatcgcc cggttgcgcc tgcatcgccc agccgctggc gaccccaccg tcgtgaataa
    23281 agaaatcgat cgccagttca tggcgtagtt cgtcatacag cggcgtatag tcacgcgacg
    23341 gtgggcgtgg tccttccggc cagacgatgc cctcttccgt taccgttggc ggcacaaagt
    23401 gagcgtcagg ttgaggaaag aagagtttgc tgtgatcgtc aaagccgcgc gatgtaaaac
    23461 cgtccagcgc ctcgccgccg aggacaatgc gctgaaaacc ggcgctgatg cgctcaacgc
    23521 gtaacacagt cagttcacgg aagcgcagat cattgcgaac gcgctgcggg tagcgggggg
    23581 agttattcat tgttatcgcc ttcgtgatag taatcagat
//
LOCUS       DAPPNJ010000064        22785 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000064 DAPPNJ010000000
VERSION     DAPPNJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.064"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            22..495
                     /locus_tag="RPM73_002011"
     CDS             22..495
                     /locus_tag="RPM73_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6520375.1"
                     /translation="MVAAMFSVAGSALAVQKDITVTANVDAALDMTQTDNTALPKAVE
                     MQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPLTVTWGGEEI
                     KADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSIYLSQDI"
     gene            587..3271
                     /locus_tag="RPM73_002012"
     CDS             587..3271
                     /locus_tag="RPM73_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDX6520376.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3268..4356
                     /locus_tag="RPM73_002013"
     CDS             3268..4356
                     /locus_tag="RPM73_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDX6520377.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4444..5742)
                     /gene="exuT"
                     /locus_tag="RPM73_002014"
     CDS             complement(4444..5742)
                     /gene="exuT"
                     /locus_tag="RPM73_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDX6520378.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6225..7637
                     /gene="uxaC"
                     /locus_tag="RPM73_002015"
     CDS             6225..7637
                     /gene="uxaC"
                     /locus_tag="RPM73_002015"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDX6520379.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7652..9139
                     /gene="uxaA"
                     /locus_tag="RPM73_002016"
     CDS             7652..9139
                     /gene="uxaA"
                     /locus_tag="RPM73_002016"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDX6520380.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9222..9773
                     /locus_tag="RPM73_002017"
     CDS             9222..9773
                     /locus_tag="RPM73_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDX6520381.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9778..11022)
                     /gene="sstT"
                     /locus_tag="RPM73_002018"
     CDS             complement(9778..11022)
                     /gene="sstT"
                     /locus_tag="RPM73_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDX6520382.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11421..12386)
                     /gene="alx"
                     /locus_tag="RPM73_002019"
     CDS             complement(11421..12386)
                     /gene="alx"
                     /locus_tag="RPM73_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDX6520383.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12669..13655)
                     /locus_tag="RPM73_002020"
     CDS             complement(12669..13655)
                     /locus_tag="RPM73_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX6520384.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13734..14417)
                     /locus_tag="RPM73_002021"
     CDS             complement(13734..14417)
                     /locus_tag="RPM73_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDX6520385.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(14494..14997)
                     /locus_tag="RPM73_002022"
     CDS             complement(14494..14997)
                     /locus_tag="RPM73_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDX6520386.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15082..16218
                     /gene="rlmG"
                     /locus_tag="RPM73_002023"
     CDS             15082..16218
                     /gene="rlmG"
                     /locus_tag="RPM73_002023"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDX6520387.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            16409..16492
                     /locus_tag="RPM73_002024"
                     /pseudo
     CDS             16409..16492
                     /locus_tag="RPM73_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            16489..16905
                     /gene="higA"
                     /locus_tag="RPM73_002025"
     CDS             16489..16905
                     /gene="higA"
                     /locus_tag="RPM73_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDX6520388.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16950..18968)
                     /gene="fadH"
                     /locus_tag="RPM73_002026"
     CDS             complement(16950..18968)
                     /gene="fadH"
                     /locus_tag="RPM73_002026"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDX6520389.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDLWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19294..21645)
                     /gene="ygjK"
                     /locus_tag="RPM73_002027"
     CDS             complement(19294..21645)
                     /gene="ygjK"
                     /locus_tag="RPM73_002027"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDX6520390.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21662..22732)
                     /gene="ygjJ"
                     /locus_tag="RPM73_002028"
     CDS             complement(21662..22732)
                     /gene="ygjJ"
                     /locus_tag="RPM73_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDX6520391.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5654 a         5860 c         5931 g         5340 t
ORIGIN      
        1 aaggtatttg caaaatctct tctggtcgca gcgatgtttt ctgttgcagg ctccgcattg
       61 gcagtacaaa aggatattac tgtaacggcc aacgttgatg ccgctctgga tatgacacag
      121 accgataaca ccgcgttgcc gaaagcagta gaaatgcaat atctgccggg gcagggtctg
      181 caatcttacc agttgatgac caaaatctgg tctaacgacg ttaccaaaga tgtaaaaatg
      241 cagttagttt ccccggcgca actggttcaa agcctggatg ctagcaagat tgttccgctg
      301 acagtaacct ggggcggtga agaaattaaa gctgatgcgg caaccacttt taccgcaacc
      361 aaaatctttg cttccgatgc gttaactaac ggttctctgg ctaaaaacct gatgtttgct
      421 caaaccacca aaggtgttct ggagacaggc atctaccgtg gtgtagtaag catttatctg
      481 tctcaggata tctaatccag ataacagaat taacacgtaa caaagggagg ggtagccctc
      541 ccttatttta actgttttag tatttgttat ggatttcagg attgccatgg ataaaaaatt
      601 actggctctt ttgatcctgg cgagtctcag cccggcagag gcgacattaa ccaaaattcc
      661 cgcagggttt gaggttattg ctcagggaca gcaggagtat atcgaggttt atttttcagg
      721 gaaaagtctc ggtaaatatt atgcaatggt taatcttgat accgtaacat ttcttgatcc
      781 agcaagtcta tataacaagc tggaactgga tgtagacgat cagaaaatcg cgcatatagt
      841 gaaagaaaaa ttatcgcagc cgctagctcg ccacggtgaa ttggcttgcg gttatgtacg
      901 tactgactca gggtgtggtt ttctgaatac cgatacgctg gaaataatct ataatgatga
      961 agaaagttcg gcaacgttgt ttattaatcc gcaatggaat tcagctttcg atgcgaagtc
     1021 attatattta aatccagaca aaaatactgt taatgctttt atacatcagc aagacatcaa
     1081 tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg cgctgggaat
     1141 aacagaaaat agttatattg gtgcacactg gaatttcaac ggttatgatg cagatgatgt
     1201 cagtgacagt aatgctgacg tcagcgatct ctattatcgt tatgattttt tacgtcgtta
     1261 ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag gcgggaactt
     1321 tacctttaac tttctgccac tcggtgcaat tgatgggatg cgtatcgggt cgacactgag
     1381 ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc tttcgcgcaa
     1441 ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc tcaatagtgg
     1501 ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg cattaaaagt
     1561 ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa ccggcggtct
     1621 gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac aggtttctga
     1681 tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc aatatgagct
     1741 ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta ataactggac
     1801 ggcagattta ggcggggtgg ggaatcttgc aatcagcgcc agcgtgttcc gtaacgatga
     1861 cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc cgacgttggg
     1921 cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag agagctataa
     1981 cgccttaagc tgttatgaaa gtatttccgc gacggtttca cagaattttg tcggctggaa
     2041 tatgatgctg ggttataccc gcacacaaaa taacactgat gatagtttgc gttgggataa
     2101 acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta tctctgaaac
     2161 tgtacaactt agcgcttccc gcgcttttgt gatgcgtgac tggattttga gtacgtcagt
     2221 cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct tgtacttatc
     2281 gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt caacaaatgt
     2341 gtctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc agttatcgca
     2401 taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg gaggcctgaa
     2461 caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg gaaatgtcta
     2521 cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg cctttacggg
     2581 cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag ccagtggtac
     2641 agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac aggatgaaga
     2701 gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc agcttggtca
     2761 aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg aggttaacga
     2821 cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag ggaatcgtga
     2881 attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct ttaactacaa
     2941 ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa
     3001 cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta acggcagcga
     3061 aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactaa gtgttgtaaa
     3121 gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga cttattcaca
     3181 attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac gtggaaacgt
     3241 tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc gatattgggc
     3301 ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga aattacgtat
     3361 gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc ggcaggaaat
     3421 aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg tacagctaat
     3481 ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat gcgttggccg
     3541 gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc gccaggatgc
     3601 aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc atgtacagaa
     3661 gatggtcaac attattatat atacgataca aagtttctta cgctctacct tgagcagaca
     3721 gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt acattcgaac
     3781 agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc caaccatatt
     3841 gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga cttgcatcca
     3901 acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga catgtgcctg
     3961 tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga tgaagggcga
     4021 ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg gaccaccacc
     4081 gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac cggtgggcaa
     4141 attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa acgtgtgcgt
     4201 ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc cttgactctg
     4261 gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg caaattgtca
     4321 gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaagcgga caaacgcttt
     4381 tttctatata aacgacgaag ccgtctgata aagcggcttc gtcattacaa cttaagttaa
     4441 acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa tcgccggttt
     4501 gttctgcaac acggtccaga taaccagcgc acctaacagg tcaaatactg ccagaactgc
     4561 gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg caaacagcgt
     4621 acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt cgttacgacc
     4681 gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg caaaaccacc
     4741 gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca tacccgggcc
     4801 aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca ggttcacacc
     4861 aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga ggtcagcaaa
     4921 cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt aaactttaaa
     4981 catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca ggaaacgcgg
     5041 cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca ttttcttcgc
     5101 cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt cttcatcggt
     5161 cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg cccagataaa
     5221 gctcaatgca ccggagatga taaatgccat ctgccagctg tgcattacga ttgcccatac
     5281 caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt aacctaccgc
     5341 gatggaacgc tctttcgccg gaaaccattc ggagctggct ttcagacccg ccgggatcat
     5401 cgctgcttcc gcggcaccga ctgcaccacg agcaatcgcc aggccacccc agctaccagc
     5461 cagcgcggtt gcaccacaga atacggccca tagtacagca aacattgcat aaccgatttt
     5521 cgtacccagc acatccagta catagcccgc taccggttgc atgaccgtat aagcagcaga
     5581 ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt ccatcagagt
     5641 tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc caagcgtcac
     5701 cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc tcatcgtttt
     5761 gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt tagttacgac
     5821 cgggtaaccg aaaaaaaacc ggcacgcccc gaacgttgcc atgaaagttt cgtgctaatt
     5881 cggcttccgt accggttaac ccgattcatc taacaaatga atgaatcggc aatttgcatt
     5941 ccgtcgactt atgatttgcg acggcagaaa gataacttgt catacaactt taaaaggtga
     6001 gagccatcac aaatgtgcga atatttgtag ggacattacc tgacgacagc gaggccagta
     6061 ctggcgcggc ctgcagagag atttaccact ttgagagtaa tttttttaac tacgtttatt
     6121 gatctaactc acgaaaatat cttcggactc tggaaattgg tgtgataact ttgtcagcat
     6181 cgcaccataa gcaagctagc tcactcgttg agaggaagac gaaaatgact ccgtttatga
     6241 ctgaagattt cctgttagat accgaatttg cccgccgtct gtatcacgac tacgcaaaag
     6301 accagccgat tttcgattac cattgccatt tgccgccgca gcagattgcg gaagactatc
     6361 gttttaaaaa cctgtatgac atctggctga aaggcgatca ctacaaatgg cgcgctatgc
     6421 gtaccaacgg tgtggctgag cgtctgtgta ctggtgatgc gtctgaccgt gaaaaatttg
     6481 acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc gttataccac tggacgcacc
     6541 tcgaactgcg tcgtccgttc ggtatcactg gcaaattgct ttctccgtca actgccgatg
     6601 aaatctggaa cgaatgtaac gaactgctgg cgcaggataa cttctccgcg cgcggcatca
     6661 tgcagcagat gaacgtgaaa atggtcggca ccaccgatga cccgatcgat tctctggagc
     6721 atcacgcaga gatcgccaaa gacggctctt tcaccatcaa agtgctgccg agctggcgtc
     6781 cggacaaagc ctttaacatt gaacaggcga cctttaacga ctacatggcg aagctgggcg
     6841 aagtttccga taccgacatt cgccgctttg ctgacctgca aactgccttg accaaacgtc
     6901 tggatcactt cgccgctcac ggctgtaaag tgtctgacca tgcgctggac gtggtgatgt
     6961 ttgctgaagc gaacgaagcg gaactggaca gcattctggc gcgccgtctg gctggcgaac
     7021 ccctgagcga gcacgaagtg gcacagttca aaactgcggt actggtgttc cttggtgccg
     7081 aatatgcacg tcgcggctgg gtacagcagt accacattgg cgcgctgcgt aataacaacc
     7141 tgcgtcagtt caaactgctg gggccggatg taggctttga ctccatcaac gaccgtccga
     7201 tggcggaaga gttgtctaag ctgctgagca agcagaacga agaaaacctg ctgccgaaaa
     7261 ccattctcta ctgcctgaac ccgcgcgata acgaagtgct gggcaccatg atcggtaact
     7321 tccagggcga aggtatgccg ggcaaaatgc agttcggttc cggctggtgg tttaacgatc
     7381 agaaagacgg tatggaacgt cagatgaccc aactggcgca gctcggtttg ctgagccgct
     7441 ttgtcggtat gctgactgac agccgtagct tcctgtcata cacccgtcac gaatacttcc
     7501 gccgcattct gtgccagatg atcggtcgct gggtggaagc gggcgaagca ccggcggaca
     7561 tcaacctgct gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt gactacttcg
     7621 ccattgaact gaactaaggt ctggggttga tatgcaatac atcaagatcc atgcgctgga
     7681 taacgtcgcg gtcgctttag cggatttggc tgaaggcaca gaagtcagtg tcgataacca
     7741 gactgttacg ctgcgccagg atgttgctcg tggacataaa tttgcgttaa cggatatcgc
     7801 aaaaggggcc aacgtcatca aatatggcct gccgattggt tatgcattgg cggatattgc
     7861 ggcgggggaa cacgtccacg cccacaatac gcgcacgaat ctgagcgatc tggatcagta
     7921 tcgctatcaa cctgattttc aggatctgcc tgcgcaagcg gcagatcgtg aagtgcagat
     7981 ctatcgtcgc gctaacggcg atgtcggggt gcgtaacgag ctgtggatcc tgccaaccgt
     8041 gggctgtgtc aacggcatcg cgcggcagat ccaaaatcgt ttcctgaaag aaaccaacaa
     8101 cgctgaaggt accgacggcg tgttcctctt cagccacacc tacggctgct cacaactggg
     8161 cgacgatcac atcaataccc gcaccatgct gcaaaacatg gtgcgccacc cgaacgcggg
     8221 cgcagtgctg gtgattggtc tgggctgtga aaacaaccag gttgccgcat tccgtgaaac
     8281 gttgggcgat atcgatcctg aacgcgttca tttcatgatc tgccaacagc aggatgatga
     8341 gatcgaagcc gggatcgagc atttgcatca gctgtataac gtgatgcgca acgataaacg
     8401 cgagccaggc aaactcagcg aactgaagtt tggtctggag tgcggtggtt ctgacggtct
     8461 ttctggtatt actgctaacc cgatgctggg gcgtttctct gactacgtga ttgctaacgg
     8521 cggtactacc gtactgaccg aagtgccgga gatgtttggc gcagagcagt tgctgatgga
     8581 ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc atggtcaacg acttcaaaca
     8641 gtactttatt gcccatgacc agccgatcta tgaaaaccca tcgccgggga acaaagcggg
     8701 cggtatcacc acgctggaag ataaatccct tggctgtacc cagaaagcgg gttccagcgt
     8761 cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg ccggggctga acttgttaag
     8821 tgcgccgggt aacgatgccg tagcgaccag cgccctggcg ggtgcgggct gccatatggt
     8881 gctgttcagt actggtcgtg gcacgccata cggcggcttt gtaccgacgg tgaaaatcgc
     8941 taccaacagt gaactggcgg cgaagaaaaa acactggatc gactttgacg cgggtcagct
     9001 gatccacggt aaagcgatgc cgcagttgct ggaagaattt atcgacacca tcgttgagtt
     9061 tgccaacggt aaacaaacct gcaacgagcg taacgacttc cgcgaactgg cgatcttcaa
     9121 aagcggcgta acgctataaa gtcgaaatga gttgttaata aaaaacggcg tttcataatg
     9181 tgaagcgccg tttgcttttc catacctgat aaggatttat catgaccgcg tattggctgg
     9241 cccagggcgt gggtgtcatc gcctttctga ttggtatcac aacatttttc aatcgtgacg
     9301 aacgtcgctt caaaaagcag ctttcggtct atagcgccgt tattggcgta catttttttc
     9361 ttctgggcac ctatcccgct ggtgccagtg ccatccttaa tgccattcgt acattgatta
     9421 ccttacgcac gcgcagctta tgggtaatgg cgatttttat tgtgctgact ggcggaattg
     9481 gcctcgcgaa gttccatcat cctgtcgaac tattgccggt tatcggtacg attgtcagta
     9541 cctgggcgct gttccgctgt aaagggctca ccatgcgctg cgtgatgtgg ttttcaacgt
     9601 gttgctgggt gattcacaac ttctgggcgg ggtcgatagg cggcacaatg attgagggta
     9661 gttttctgct tatgaatggc ttgaatatca ttcgtttctg gcggatgcag aaaaggggaa
     9721 ttgatccgtt taaagtagag aaaacccctc ccgccgtaga cgaaaggggt taaacaatta
     9781 attacgcagg gcgctatttg ccagacgatc gtcttccgcc tggcaagctg ccgcagtgaa
     9841 cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata cgccgatgat
     9901 aaagccgacg gcaaccacct gcatggcgat atcgttcgaa ataccgaaca tattacaggc
     9961 cagcgggatc agcagcagag aaccccccgc cacgccggat gcgccacagg cacacagaga
    10021 agccaccacg ctcaacagca gcgccgtggg cagatcgacc gggataccca gcgtattaac
    10081 cgcagccagc gtcaacacgg taatggtgat tgctgcgccc gccatattga tggtggcacc
    10141 cagcggaata gaaacggaat aggtatcgcg atccagattc agcttttcac acagtgccat
    10201 attcaccgga atgttcgccg cagagctgcg ggtgaagaag gcatacacgc cgctttcgcg
    10261 caggcacagc agcaccagcg ggaacgggtt acgacgaatt ttccaccaca ccagcaatgg
    10321 gttaaccacc agcgccacca gtaacataca gccaaccagc acgaccagca gttgcgcgta
    10381 gccccacagt gtggagaaac cggtggttgc cagggtagaa gaaaccagac caaaaatacc
    10441 aatcggtgcg aagcgaatga ccagtttcac cataaaggtg acggcattcg acatatcgtt
    10501 gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg aaaccgaggc caattgccca
    10561 caccaggatc ccgatgtagt tacctttcag cagcgcgtcg atggggttgg aaaccatgct
    10621 cattaccagc ccgcgcatca cttcgacaat gcctgacggc ggcgaaatat cacccgcgct
    10681 actggataaa tgcagggtag aagggaaggc aaagctgaag actactgcgg ccagagcagc
    10741 agagaaggta cccagcagat agaggaacaa aatagggcgg atattggttt tctgcccgtg
    10801 ctggtggtta gcaatagatg ccatcaccag catcaacacc aggatggggg caacggcttt
    10861 cagtgcgccg acgaacaaag tacctaacag accaacagct tccgccgcgg gttttgagat
    10921 ccatgccaga agaatcccca gaacaaggcc gaccaggatt tgttttacca ggctgccatg
    10981 agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat cctttcattg
    11041 tgttgtcgca catccctggt gcgttctgtc gacgcatctt tcaggatgta acaaagtgtt
    11101 tgcaaggtcg agtataagga aagttacagc ttcgggaagc gaaaaatgct ggattttaaa
    11161 gagaggtcat attttgcaac ttaatggtta catttatttg acataaaaat aaagccaacg
    11221 tgcggcttcc agactgatga caacatcaaa aaaggactcg gtcagtcccc tcgccccgtt
    11281 tggggagagg gttagggtga ggggaacagg ccagcatcgg tgcgaaaaat taccctcacc
    11341 ccgaccctct ccctggaagg gagagggaga aaacggtgct catgttaatg acagggtaca
    11401 cggcttaggc agattaaaaa ttatccaccc cgctgcttat catgccgata attcacccag
    11461 gcgttgataa taaacgtcat caccagaatg ccaaacacca cgcccagcga gacggcgatt
    11521 ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag gatcaccgcc
    11581 aggccatatt tgagcatcga gaaacgctct gctacgcccg ccagcaggaa atacatcgca
    11641 cgcaggccga ggatcgcaaa caggtttgag gtcagcacaa tgaacgggtc agtggtcacg
    11701 gcgaagatag ccggaatgct atccacggcg aaaatcacgt cgctcaattc caccagaatc
    11761 agtaccagca tcagcggtgt ggcgtacagc aatccattct tacgcacaaa gaaatgctcg
    11821 ttgtcgatgg tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg
    11881 ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa cagcaggaag
    11941 gcaccgaaga tatacaggat ccagtcgaac tgtgaaatca gccagctgcc tgtgaagatc
    12001 atgatggtac gcagaacaat cgccccgagc acgccataca ccagcacgcg gcgttgtaat
    12061 gccgccggaa cagagaaata gctgaacaac atcagccaga caaagacgtt atcgaccgcc
    12121 agcgatttct caatcagata gcccgtgaga aaggccagtg cctgtggatc ggcaacggcg
    12181 cgaccttcag tttgcaccag ataccaccag aaagcggcgt taaacagtaa cgacagcgtc
    12241 acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc cccacgacgc
    12301 ccctgcaaca acaggtcgat agccagcata atggcgacaa caacagcgaa tccgccccat
    12361 agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa cggccaacgt
    12421 cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa ggtctcactt
    12481 acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt
    12541 ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt gcaggtaaca
    12601 aagtatgaca gaccattcag ccagtcaatg gtctgttaca taccttttac actgttttac
    12661 tttggcaatt atagttttac gctatctgcc ggaaaaatca ccccagtctg gcggcggatc
    12721 tcggtcagca gtttggcggt gatgcgactg accgccagcc ccggatgatc caccagatgc
    12781 tcatccacca gggtagcgaa cagctctgct tcgtagagca tggtattaat atgctgcggc
    12841 tgggtgagat cctgcatttg gctgccacgc ggcacgaagc acactttctg gcattcagac
    12901 agtttttcaa tcaccagcga tcctgcttcg ccctgaatct cgctcgccag gacagaatca
    12961 ctgacttttg agtgctgcaa ggtgacgctg aaatcaccgt aatccatcac caccacgccg
    13021 tgggcatcta caccgcttgc cagcaaactg gcggttgcct gcacgctttt cggctcacca
    13081 aataacgcca ccgccgacgc caggcagtaa aagccgatat ccataattga accgttagag
    13141 aatgacggat taaaggtgtt gggattttca ccgtccaggt aacgttgata gcgcgaggaa
    13201 tactggcaat agttgaaaaa gactttacgc aatttgccga ctttcggcag cgcctggcgc
    13261 agcaaatgaa agttcggcag gcaggcggtt ttaaatgcct caaacagcac cacctgattt
    13321 tcccgcgcac aggcaatggc ggcatccact tccgccagat tcgacgccag tggtttctcg
    13381 caaatcacat gaattttatg gctaaggaaa agttgtgtct gggaaaagtg cagggcattc
    13441 gggctggcaa tatacaccgc gtcaatggca tcgctttccg ccatcgcttc cagcgaggta
    13501 aacagatgct cgacagaaaa atcattggcg aagtgctgag cctgctcaag gctgcgggaa
    13561 tatacggcgg ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa ctggcgagtg
    13621 atccagttcg taccaatcac agcgaaacgt atcataacgc gtggatactc cataaaggga
    13681 ttctgtcgtc aatgtagcac gcctttttca tcttcgacgt gcgcagatac gcattattct
    13741 tttgccggac agccatgctc actaaacggc ccaatcatga ccgatggccc gagatatttc
    13801 ggctggcgat ggaggatttt ggcgtcaatc acggcgctga cacgagcgag tttctcccgc
    13861 agttgagtat aaaaaccgtg cttcatattg aggtcaggcg cgttaaaacc gatagcgtcg
    13921 atgctgtact gttttgccag ccagatggcg cgttggttat gaaattcttg cgagataatg
    13981 gtgatatggt tttcaccaaa aacctttttg gcacgcacta ccgaatccag cgttgagaat
    14041 ccggcatagt cgcagaagat cactttcgcc ggtacgcctt tagcgatcaa agccttctgc
    14101 atacctgatg cttcatcgta atttttacgt ccgttatcgc cacttaccag tagccatttc
    14161 acttttccgg cgtggtacaa cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta
    14221 cccggcctgg cccccagcaa taagcccacg ttacgctccg gaactgcgtt gacatcgctc
    14281 caggtcagct gcttgctggc gttgaccata acgcgatcgg caataaaaat ggtcgctcct
    14341 gctataagca acaacaaaca gccaatctta agagtccggc gagaaaaaca aatacggaga
    14401 agaaggcggg gctgcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc
    14461 taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa
    14521 ctgcgttagc cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca
    14581 gcacaactgg taaaacgctt tgttatgctc tttctccttc aggtgcgcca gttcgtgcac
    14641 cacgatcata cgtaaaaacg gttccggcgc gttgcgaaac acagtagcga cgcggatctc
    14701 tgcttttgct ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc
    14761 gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg gggcattgcg
    14821 cagaaactga ttttttagat cctgagtata ttgccagagg gctttatcgg tggcgtagtc
    14881 gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagcgt
    14941 tcgcacctgg gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac
    15001 cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa
    15061 ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta
    15121 caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa
    15181 tatttgctgc aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat
    15241 gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca
    15301 tacatcagtg aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc
    15361 gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa
    15421 gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg
    15481 tcggatacac gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggag
    15541 ctgttcgaga aagtgctcgg cccgaccacc actacgctgg catggaaaaa agcgcgcctg
    15601 attaattgca ctttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag
    15661 ctggaaggta ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctg
    15721 gatattggcg cacgcttctt tatgcaacat ctgccagaga atctcgaagg tgagattgtc
    15781 gatctcggct gtggtaacgg cgttattggc ctgacgctgc ttgataaaaa cccgcaggcg
    15841 aaagtggtgt ttgtcgatga atcgccgatg gcggtcgctt ccagccgaat gaatgttgaa
    15901 accaacatgc cagaggcgtt ggatcgctgc gagtttatga tcaacaacgc gctctccgga
    15961 gtggagcctt tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca gcaacatgcg
    16021 ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac
    16081 ggcgaactgt atatcgttgc caaccgtcac ctggattact tccataaact gaagaagatt
    16141 ttcggcaact gcaccaccat cgccacgaat aataaatttg tggtgctgaa aacagtgaag
    16201 ctggggcgtc gtcggtaaga ttgggagtta cctgccggat gcggcgttaa cgccttatcc
    16261 ggcctacgtt cagcacgtta ctcccaggcc ggataagacg cggcaagcgt cgcatccggc
    16321 aatgtctgcc cagttctgga atcgcgcttc cataaaacaa aaaatccctg tcaccatcct
    16381 ctacattctc ttgtttagcg ttgttcaacg tttatgctac atacgtgaag ttatgacgca
    16441 taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt
    16501 gccgacatct tgcaagcagg agaaaagcta actgcggtgg caccttttct ggcgggtatt
    16561 cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat
    16621 cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg
    16681 cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt
    16741 cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa
    16801 tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa
    16861 ttggcaacac gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat
    16921 gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg
    16981 gcaattgccc gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca
    17041 gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag
    17101 atcaccacat tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg
    17161 tcatcgtcaa tcttctgata acttacgctt gggatcattt tcacaccccg cgagagcagg
    17221 gtggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg
    17281 cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag
    17341 ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc
    17401 tggctggtgg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca
    17461 caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa
    17521 ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cgcgcggcac gatcccactg
    17581 gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc
    17641 agcgtcacgc ccgtcacttc gatcatccgg tggtaatagc gcagcgtttc gtaaaactcc
    17701 tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgggcatca
    17761 aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt
    17821 cccgcaccga ccaccgccag attttttttc tgcacggcag gaaggattgg cattttggtt
    17881 tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg
    17941 aggcaggcct gattgcagcc aatgcaggtg ttgatctcat cggctcgccc cgattgcgct
    18001 tttgacagca gttccgcatc agcaagaaac ggtcgtgcca tcgacaccat atcggcatcg
    18061 ccgcgcgaga gaatgtcgtc ggcaacctgc ggatcgttaa tccggttggt ggtcaccagc
    18121 ggcagcgaga cgtggccttt cagtttgcgc gtgacccaag taaatgcgcc gcgcggcacg
    18181 ggcgtggcaa tggtcggaat acgtgcttca tgccagccaa tgccggtgtt gataatggtc
    18241 gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct
    18301 tcgaccaggt cgagcatcga caaccggtag ataataatga agtcgttgcc gacgcgttcg
    18361 cgcaccgtac gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac
    18421 aggtcactac gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcggac
    18481 cccattacct ctacaccgtc gtatcctgcc tcccgtgcca gttgcgcgca gtgggcgaaa
    18541 tcgtcgatca gttgcaggat ctcttcatgc gtgagttcgt gaggaacgaa acggttgatg
    18601 ggggcctgca atgcggaggg agcgaccaga tgcggttggt agctgtagcg cccggtatgc
    18661 aaaatttgca gggctatttt gccgccttcc tgatgtaccg cttcggtaat ggtgcgatgg
    18721 tgtgggatct ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctgttaaa
    18781 tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa
    18841 aacgctgcca gccgctcggc accgtccggg tattcctcca gcccggtgtg cattgagccc
    18901 atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg
    18961 tagctcataa aattgtccag tatgtaaaat aattgttatg tggtcggatg agttctaatt
    19021 tagccgtcgc cggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag
    19081 aatacgcccg gtggtaacgc cggatgcgac gcttgccgcg ttttatccgg cctgggggat
    19141 tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagtggcacg gtgcagatgc
    19201 cggatgcgac gcttgtcgcg ttttatccgg ccaggaggat tgcacatgta gggcggataa
    19261 ggcgtttacg ccgcatccgc cagcagttgc aaattattgc ttacggaaaa aatcgttata
    19321 caacatatac aaatgcgcgg cactccagga gaaatttggt gcgccttgct gtgcgccagt
    19381 cagcgggttg taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgacggaa
    19441 gaacgtatcc gccagtttca gggcatcatc gcgataaccg taacgctcca tccctttcag
    19501 accaaaccag aactgatcca cccatacgcg cccacgccag tagatatcag cgccaaaagc
    19561 cggattggtt aacgccgccg ttcccagcgg gacaaaggtg ttgaactctt taggatcgag
    19621 catcaccttc accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga
    19681 ccagccttcc ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag
    19741 tggtttatct tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt
    19801 gttgatgtag tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt
    19861 accgaggatg gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg
    19921 atccaccgac tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc
    19981 ggcgaatttc accgtccagt cgctacgttt gccgccatta gcgacatatt tatccaactg
    20041 ttctttgtcg ataaaaccaa agacggcggc atcatcacgc cccgattccc acgacgcagc
    20101 gacctgtgcc ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt
    20161 gttcagccca gactgcgtct cttctttgtt gcctttcttc accgtaaaca gcatctcgcc
    20221 gctctcagtg ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt
    20281 gtgatcgcgg ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc
    20341 caaccaggct ttatcctggg tgacgttgta cacttccatc accgaccagg cggcaaggct
    20401 gggtttggta ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt
    20461 ccacgctatc aggtcgggga caaagcccac atcctgcgga cgcacgctat cgccaggctg
    20521 gatctgccag gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc
    20581 catcgcaaac gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg
    20641 cccggtcacc gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agcgccagtt
    20701 accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg
    20761 attggttaac cctttcttca ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa
    20821 cgccggacgc gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt
    20881 cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt
    20941 aaagcgatta ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga
    21001 ttcgccggag gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc
    21061 atcacgggtg gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc
    21121 ggaaagcggt ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag
    21181 atccagcggt ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa
    21241 gcgcagagtc atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg
    21301 aatactgtat gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg
    21361 gtcgaaattg ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa
    21421 gccgcccatg gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa
    21481 aaacggatta aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc
    21541 agtacggtta atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat
    21601 cagcagagcg caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt
    21661 attaccagta gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt
    21721 tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga
    21781 taccaaactg catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt
    21841 aggtgtaagc ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca
    21901 tcccactttc acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc
    21961 gagcggcaaa gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga
    22021 tttccagacc gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca
    22081 tcattgccgg ataggtgtat ttgtcgtcat agcgatcgta cttgccaaaa tgtagccagg
    22141 ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac gctataacga aagttgccgc
    22201 ttaagttctg caccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca
    22261 gatcgggggt aaagtcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac
    22321 tgagcatgat ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc
    22381 cctcgaaacc accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca
    22441 cggtccagcc ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac
    22501 cttcaatata ggtaccgtcg ctgtatttat gtttattctc accctctaaa tccatatatc
    22561 caccaacccc taattcgccg taaaaacgaa atgaaggaga agatgtcgag gtaattgcag
    22621 gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc tcctgccgca gaaaaggcgt
    22681 agcaaaacgg cagagcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa
    22741 tccatgggct aaagaaaaag tacagccttt tatcgttgta attat
//
LOCUS       DAPPNJ010000065        21860 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000065 DAPPNJ010000000
VERSION     DAPPNJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.065"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            13..714
                     /locus_tag="RPM73_002029"
     CDS             13..714
                     /locus_tag="RPM73_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX6520392.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            739..3339
                     /locus_tag="RPM73_002030"
     CDS             739..3339
                     /locus_tag="RPM73_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDX6520393.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQVKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3330..4400
                     /locus_tag="RPM73_002031"
     CDS             3330..4400
                     /locus_tag="RPM73_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6520394.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4412..4954
                     /locus_tag="RPM73_002032"
     CDS             4412..4954
                     /locus_tag="RPM73_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6520395.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4962..5477
                     /locus_tag="RPM73_002033"
     CDS             4962..5477
                     /locus_tag="RPM73_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6520396.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5443..6180
                     /locus_tag="RPM73_002034"
     CDS             5443..6180
                     /locus_tag="RPM73_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDX6520397.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADKESIAILLTNTSQESWLINSKINRPTRWAGGEVSTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     VIAQCHVAWRAINDYGGLSAKKEQNLP"
     gene            6291..7301
                     /gene="pyrD"
                     /locus_tag="RPM73_002035"
     CDS             6291..7301
                     /gene="pyrD"
                     /locus_tag="RPM73_002035"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDX6520398.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMYHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7475..8017
                     /gene="zapC"
                     /locus_tag="RPM73_002036"
     CDS             7475..8017
                     /gene="zapC"
                     /locus_tag="RPM73_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDX6520399.1"
                     /translation="MRIKPDDNWRWYYDEGHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8014..9123)
                     /gene="ycbX"
                     /locus_tag="RPM73_002037"
     CDS             complement(8014..9123)
                     /gene="ycbX"
                     /locus_tag="RPM73_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDX6520400.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9367..11475
                     /gene="rlmKL"
                     /locus_tag="RPM73_002038"
     CDS             9367..11475
                     /gene="rlmKL"
                     /locus_tag="RPM73_002038"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDX6520401.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11486..13393
                     /locus_tag="RPM73_002039"
     CDS             11486..13393
                     /locus_tag="RPM73_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6520402.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13523..14776
                     /gene="pqiA"
                     /locus_tag="RPM73_002040"
     CDS             13523..14776
                     /gene="pqiA"
                     /locus_tag="RPM73_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDX6520403.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14781..16421
                     /gene="pqiB"
                     /locus_tag="RPM73_002041"
     CDS             14781..16421
                     /gene="pqiB"
                     /locus_tag="RPM73_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDX6520404.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16418..16981
                     /gene="pqiC"
                     /locus_tag="RPM73_002042"
     CDS             16418..16981
                     /gene="pqiC"
                     /locus_tag="RPM73_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDX6520405.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17237..17404
                     /gene="rmf"
                     /locus_tag="RPM73_002043"
     CDS             17237..17404
                     /gene="rmf"
                     /locus_tag="RPM73_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDX6520406.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17474..17992)
                     /gene="fabA"
                     /locus_tag="RPM73_002044"
     CDS             complement(17474..17992)
                     /gene="fabA"
                     /locus_tag="RPM73_002044"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDX6520407.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18061..19821)
                     /locus_tag="RPM73_002045"
     CDS             complement(18061..19821)
                     /locus_tag="RPM73_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDX6520408.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20007..20459
                     /gene="matP"
                     /locus_tag="RPM73_002046"
     CDS             20007..20459
                     /gene="matP"
                     /locus_tag="RPM73_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDX6520409.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20535..21575)
                     /gene="ompA"
                     /locus_tag="RPM73_002047"
     CDS             complement(20535..21575)
                     /gene="ompA"
                     /locus_tag="RPM73_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDX6520410.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         5562 a         5153 c         5765 g         5380 t
ORIGIN      
        1 gagtgactat ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca
       61 atattactca gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca
      121 acacgtcttg tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt
      181 ccggatgttc cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca
      241 gattttatta ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc
      301 attatgtaca ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta
      361 cgggcagtcc cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa
      421 agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg
      481 gcgaaactgc gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat
      541 ttcattaccc tgacagacct gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc
      601 ccctttgata aataccaatt ttctctgcca aagggggcca aaaatagcag cgtaacgtat
      661 cgaaccatca atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc
      721 ttcagttaag agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt
      781 ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa
      841 gctgaaacct ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg
      901 tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg
      961 aatcagacaa ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg
     1021 attgccgcct gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt
     1081 ccggctttta aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat
     1141 gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc
     1201 atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg
     1261 ctattactgg gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat
     1321 tctggcgaca gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg
     1381 cgcaacaatt caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc
     1441 agctatttgc agcgggcggt tattccacta aaaggcgaac tgaccgttgg tgatgattat
     1501 actgcaggcg attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac
     1561 aacatgctgc cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc
     1621 aatgcccaga taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct
     1681 ggtgcttttg aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa
     1741 atcaaagaag cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta
     1801 ctccagcgtc aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt
     1861 aatgaacagc aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga
     1921 acgacatggt atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg
     1981 ggatttaacc tcggcgattt cggagcaatt tcgttcgatg tgacccaggt gaagagtacg
     2041 ctggcagacc aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc
     2101 aaccaattgg gcactaactt tcaattgatg ggctatcgct attcgacgtc gggtttctac
     2161 actctttccg acaccatgta taaacacatg gatggctacg aatttaatga cggtgatgat
     2221 gaagatacgc cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg
     2281 caggtcaata tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag
     2341 caaacttact ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa
     2401 attaaagatc tctcgctggg ggtttcctgg aactacagta agtcccgtgg tcaacctgat
     2461 gctgatcagg tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt
     2521 aatgatagct ataccaggaa aaaaaattac gcctggatga cctctaacac cagtatcgat
     2581 aacgaagggc acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac
     2641 ctgagctaca gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc
     2701 agcatggact acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac
     2761 ggcagtcaac aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc
     2821 attaccctgg ggcaatcgct gggggaaact aacgtgctga ttgcagcacc aggcgcagag
     2881 aatactcgtg tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc
     2941 ccctatgcca cttcttatcg ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt
     3001 aacgtggatc ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg
     3061 gcggagttca atgcccatgc gggagcaagg gtattaatga aaacatcaaa gcagggtata
     3121 ccgctgcgtt ttggcgcgat agcgacgctg gacggcatac agactaatag cggcattatt
     3181 gatgatgatg gctcgctcta tatgtccggt ttgcccgctc agggagcgat aactgtacgc
     3241 tggggcgaag ctcccgatca aatttgtcat atcagttacc agcttaccga acaacaaatt
     3301 aactctgcga ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt
     3361 tattactacg actatttttt gccgccgtct taatgctatg gtgcgctcaa actgctgctt
     3421 atagcgggca gtgtcatacc actcagggga atccgtatat tggcgtcaat tttggcgtta
     3481 aaaccctgga ggaagaagaa aatacggctg gagtagttaa agacaaattt tatcagtgga
     3541 acgaatcgaa tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc
     3601 gatgggcatt cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta
     3661 atgactatct tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc
     3721 ctttcgaaaa cgtgggcaca ggggcagata cacgatggca tatttgcgat cccggcggtc
     3781 aacgtttagg tggccagggg gctagcggta atagcggtag cttttccctg aaaatattgc
     3841 agccgttcgt tggttcggtc gtcattcctc ctatggcgct ggcgcgatta tttgaatgct
     3901 acaacatacc cgcaggtgat tcctgcacga ctacaggtac atcggtttta gtgtattacc
     3961 tgtctggtac gatcaattca cttggctcat gttccgtcaa tgccggagag actatcgagg
     4021 tcgatctggg cgacgtattt gcggctaact ttcgtgttgt agggcataaa ccccttgggg
     4081 ccagaacggc agaactcgca attccagtca ggtgtaacac gggaaacgcg gggttagtta
     4141 acgtcaacct gagtctgacg gcaaccacag accccagcta tccccaggcg attaagacgt
     4201 cacgtcctgg cgtgggcgtg gtggtgaccg atagccagaa caacattatt tcccctgctg
     4261 gtggaacatt accgctctct attcctgatg atgcagacag tatcgcgcga atgaatgtct
     4321 atccagtcag cacgacaggt gtaccaccag aaaccgggcg atttgaagcc acggcaacgg
     4381 tgagaataaa ttttgattaa cgcgtgaacg tatgaagaaa aaaacgatat atcagtacgt
     4441 tattttgttc tttagccttc ttaacatcca tgtcgggatg gctgggcctg aacaagtcag
     4501 tatgcatatt tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt
     4561 acaaaatatt catattggcg attttaatat cagtgatttt caggccgcga ataccgtaag
     4621 cactgcagct gatttgaata tcgatatcac cggttgtgcc gctggtatta ctggcgcgga
     4681 cgtccttttt agcggcgagg ctgacaccct tgcgccgaca ctgcttaaac taactgacac
     4741 aggcggaagc ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca
     4801 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4861 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4921 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4981 ggattctgtg cttactggga ttaacgggga gtagtgagtt atttgctcat gatggcacgg
     5041 tcaacattag cggatcgttt cgtcgtaata catgcgtgct ggcacaggat agcaagcaaa
     5101 tcaatgtgca gttaggcgat gtctcgctga cacgtttttc tcacgggaat tatggcccgg
     5161 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca
     5221 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtatgg
     5281 atgcagccag cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     5341 accaggcgag taaagactac agcctacaca gcggtaaggt gccattaact ttttatgcgc
     5401 aattacgacc tgtcaatagt gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     5461 tacttcatta tgactaatac gtggaacaga ttggcgctct tgatttttgc cgttttatcg
     5521 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     5581 ccggcagaca aagaatcgat agctatttta ctgactaata ccagtcagga atcctggctt
     5641 attaacagta aaatcaatcg cccaacgcgt tgggccgggg gggaagtgtc gacagtgcca
     5701 gcaccattac tggccgctcc accgcttatt ctcctgaagc ccggtactac gggcacgttg
     5761 cgcttgctga gaacggaaag cgacatcttg ccagtggatc gcgaaacgct atttgagtta
     5821 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5881 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5941 caacaattac gctggacacg gaattcgcag ggtgtgcaac tcactaaccc aacgccttac
     6001 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagttgtg
     6061 ccacctaaaa gccagcgtca gacaagctgg tgtcaggtaa tagcacagtg tcatgttgcc
     6121 tggcgggcga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     6181 aacaggttcg gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     6241 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     6301 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     6361 agcaattacg ccgtattaca ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg
     6421 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     6481 ttgataaaga cggggagtgc attgacgcgt taggcgcgat gggatttgga tcgatcgaga
     6541 tcggtaccgt cacgccacgt ccacagccag gtaatgacaa gccgcgtctc tttcgtctgg
     6601 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6661 tagagaacgt aaaaaaggcc cattatgacg gcgtcctggg tattaacatc ggcaaaaata
     6721 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6781 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6841 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6901 tgcaagcgat gtaccataaa tatgtgccga tcgcagtgaa gatcgcgccc gatctttctg
     6961 aagaagaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     7021 caaccaatac cacactcgat cgttctcttg ttcagggaat gaaaaattgc gatcaaaccg
     7081 gtggcttaag tggtcgtccg cttcagttaa aaagcaccga aattattcgc cgcttgtcac
     7141 tggaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     7201 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     7261 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     7321 tttataagat aatcagggct ttattttcag ccctggttgt tttatattca tcgctgttgc
     7381 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     7441 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     7501 gcgttggtat tacgatgaag ggcacgatcg aatgatgctc gatttagcca atggtatgct
     7561 atttcgctca cgttttgcgc gcaagatgtt gactccagat gccttttctc ccgcaggctt
     7621 ttgcgttgac gacgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7681 atctaaagaa caaaaagccg aactggtgct gaatgcactg gtagcaattc gctatctcaa
     7741 accgcaaatg ccgaaaagct ggcattttgt ttcccatggt gaaatgtggg tgcccatgcc
     7801 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7861 tgaatctggc gaaaatgccg cactatgcct gctggcacaa ccctgcgttg ttattgcggg
     7921 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7981 taatgttgac agcttcagcc tcgaacaggc tgtctaacgc gccaacttaa gtgcagtctt
     8041 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     8101 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     8161 ataagggata cgaattccct gattttctaa ttgctccagc aacacctgtt ggttatttcc
     8221 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     8281 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     8341 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     8401 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     8461 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     8521 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     8581 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     8641 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8701 aaatgacagg ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8761 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8821 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8881 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8941 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     9001 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     9061 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcac
     9121 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     9181 ctataatgcg caacaatttt cttagcgcct gtcccattag gctattttat ttgtcagttt
     9241 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctg
     9301 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     9361 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     9421 gaactggaaa acctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     9481 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     9541 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     9601 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9661 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9721 gctttcacgc ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9781 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9841 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9901 gccgctattg tgatgcgatc cggctggcag ccaggaacac cgctgctcga tccgatgtgt
     9961 ggttccggta cgttgctgat tgaagcggcg atgctagcga ccgatcgcgc accaggcttg
    10021 caccgtgggc gttggggctt tagcggctgg gtgcagcatg atgaagatat ctggcaggaa
    10081 gtgaaagcgg aagcgcaaac tcgtgcccgt aaaggcctgg ctgagtatag ctctcatttt
    10141 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
    10201 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
    10261 ccgaaagggc cgtacggcac agtgttgagc aacccgccat acggggaacg tctggacagc
    10321 gaaccggcgc tgattgcgct gcatagcctg ctgggtcgga tcatgaaaaa ccagtttggt
    10381 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgctt gcagctgcgt
    10441 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
    10501 gttgccgaaa gcactccaga cagcaaaccg gcgatggtag cggaagacta cgccaaccgc
    10561 ctgcgtaaga acctcaaaaa attcgagaag tgggctcgcc aggaaggaat tgaatgttac
    10621 cgcctgtatg acgccgatct accggaatat aacgttgccg ttgaccgtta tgccgactgg
    10681 gtggtggtgc aggagtatgc gccgccaaaa actattgatg ctcacaaagc gcgtcagcgt
    10741 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10801 ctgaaaaccc gtgaacgcca gaagggcaaa aatcagtacc agaaactggg cgagaagggc
    10861 gagtttcttg aagttaccga atataacgct cacttgttgg tgaacctgac ggattacctc
    10921 gataccggtc tgtttctcga tcaccgtatc gcccgtcgta tgctcggtca gatgagcaaa
    10981 ggcaaagatt tcctcaacct gttctcttat accggcagcg ccaccgtgca tgcgggatta
    11041 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga gtgggcagaa
    11101 cgcaacctgc gtttgaatgg cctgaccggg cgtgcgcatc gcctgattca ggccgattgc
    11161 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    11221 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    11281 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaataaa
    11341 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    11401 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    11461 attaccgcag cctaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc
    11521 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt
    11581 ttgtctggtg ggccgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga
    11641 acaagggctg gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca
    11701 acaggatccg ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga
    11761 agaacaagcg gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc
    11821 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct
    11881 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt
    11941 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
    12001 gagtaatccg cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat
    12061 cgactggctg gaagggtttt tgaaaacttt caacgggacg attattttca tctcccacga
    12121 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
    12181 gacctatcca gggaattacg accagtacct gctggaaaaa gaagaagccc tgcgcgtgga
    12241 agaattacaa aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca
    12301 ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg
    12361 tcgcgagcgt ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc
    12421 cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa
    12481 gcaactggtg aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg
    12541 tccgaatggg tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga
    12601 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
    12661 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
    12721 ggttaacggc aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa
    12781 acgggcgatg acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc
    12841 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
    12901 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
    12961 ggttagccac gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg
    13021 cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca
    13081 gtatgtggcg ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc
    13141 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca
    13201 gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc
    13261 ggatgcttcc ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc
    13321 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
    13381 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt
    13441 gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat
    13501 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
    13561 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
    13621 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
    13681 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
    13741 gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc
    13801 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
    13861 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
    13921 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
    13981 tggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
    14041 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
    14101 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg
    14161 cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
    14221 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
    14281 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
    14341 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
    14401 cattctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat
    14461 tatggtgcca acgttaaaga tgatcgccat cgcgtggcta tgttgggatg ccaaagggca
    14521 tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
    14581 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
    14641 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
    14701 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
    14761 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
    14821 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc
    14881 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
    14941 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
    15001 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
    15061 tggaaaaatt gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg
    15121 aagggattag cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg
    15181 cgaaaggcag caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg
    15241 atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag
    15301 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacac
    15361 aaaaacgcaa tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca
    15421 acaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca gcagggatgc
    15481 gtgtggagat gggctcattg acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg
    15541 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
    15601 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
    15661 attcggtacg cggtctgcaa ccgggagctc cggtagagtt ccggggtatt cgcctgggta
    15721 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
    15781 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
    15841 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
    15901 ccggaaacct ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg
    15961 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtt agcggcggcc
    16021 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga
    16081 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
    16141 tgcaaacgac gctggatagc atgaacaaga tcctcgctag ccagtcgatg cagcagttgc
    16201 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
    16261 gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
    16321 aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga
    16381 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
    16441 tgcagcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt
    16501 acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt
    16561 agagcaggtc actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
    16621 tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg
    16681 caacaccctg gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc
    16741 tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga
    16801 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
    16861 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
    16921 ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta
    16981 attataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt
    17041 tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt
    17101 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
    17161 attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt
    17221 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
    17281 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
    17341 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc
    17401 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc
    17461 tgtctgatta taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg
    17521 cggtatagat aagacgacca tcaaccagca cttcgccatc cgccaggccc ataatcagac
    17581 gacggttaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta
    17641 cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca
    17701 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    17761 taaccggatc gccaataaag tggcatccga agaaccacag atccggattg atatccagtt
    17821 ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt
    17881 ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc
    17941 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    18001 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    18061 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    18121 ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    18181 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    18241 gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    18301 aacgttagct gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat
    18361 agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg
    18421 atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat
    18481 cagggcgcag agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc
    18541 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    18601 cgcttgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc
    18661 gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc
    18721 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    18781 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc
    18841 gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa
    18901 ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct
    18961 aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca
    19021 ggcatccgct cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    19081 ccactgggtt actgactctg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    19141 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    19201 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    19261 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    19321 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    19381 atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag
    19441 ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    19501 tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac
    19561 cagttggcct gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc
    19621 agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    19681 ctccagtgcg aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    19741 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    19801 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    19861 accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg catgggcgtt
    19921 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19981 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    20041 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca
    20101 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg
    20161 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    20221 aaaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    20281 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg
    20341 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    20401 acgcgaataa aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac
    20461 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    20521 taccagacga gaacttaagc ttgcggctga gttacaacgt ctttgatacc tttaacttcg
    20581 atctctacgc gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg
    20641 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20701 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20761 ccctggttgt aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg
    20821 tctttcggat ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20881 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20941 gtctgtactt ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga
    21001 ccgaaacggt aggaaacacc caggctcagc atgccgttgt ccggacgagt gccgatggtg
    21061 tgtgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    21121 atcgcgtact caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca
    21181 taaacgttgg atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc
    21241 aggtcgtcag tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat
    21301 gcaccgtttt caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca
    21361 aagccaacat acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca
    21421 tgggtcgggc cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta
    21481 gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc
    21541 agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa
    21601 atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct
    21661 caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct
    21721 acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg
    21781 tataaggtat gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc
    21841 gcttcagaca agcctccgca
//
LOCUS       DAPPNJ010000066        21687 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000066 DAPPNJ010000000
VERSION     DAPPNJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.066"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..378)
                     /gene="yeiL"
                     /locus_tag="RPM73_002048"
     CDS             complement(<1..378)
                     /gene="yeiL"
                     /locus_tag="RPM73_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDX6520411.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLR"
     gene            508..1449
                     /gene="rihB"
                     /locus_tag="RPM73_002049"
     CDS             508..1449
                     /gene="rihB"
                     /locus_tag="RPM73_002049"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDX6520412.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1549..2799
                     /locus_tag="RPM73_002050"
     CDS             1549..2799
                     /locus_tag="RPM73_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDX6520413.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPQGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2855..3943)
                     /locus_tag="RPM73_002051"
     CDS             complement(2855..3943)
                     /locus_tag="RPM73_002051"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX6520414.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3947..4804)
                     /gene="nfo"
                     /locus_tag="RPM73_002052"
     CDS             complement(3947..4804)
                     /gene="nfo"
                     /locus_tag="RPM73_002052"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDX6520415.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4878..5927)
                     /locus_tag="RPM73_002053"
     CDS             complement(4878..5927)
                     /locus_tag="RPM73_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDX6520416.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            6026..6907
                     /locus_tag="RPM73_002054"
     CDS             6026..6907
                     /locus_tag="RPM73_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520417.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            7112..8581
                     /gene="lysP"
                     /locus_tag="RPM73_002055"
     CDS             7112..8581
                     /gene="lysP"
                     /locus_tag="RPM73_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDX6520418.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8874..10865
                     /gene="cirA"
                     /locus_tag="RPM73_002056"
     CDS             8874..10865
                     /gene="cirA"
                     /locus_tag="RPM73_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDX6520419.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(10897..11733)
                     /gene="yeiG"
                     /locus_tag="RPM73_002057"
     CDS             complement(10897..11733)
                     /gene="yeiG"
                     /locus_tag="RPM73_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDX6520420.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11991..12659
                     /gene="folE"
                     /locus_tag="RPM73_002058"
     CDS             11991..12659
                     /gene="folE"
                     /locus_tag="RPM73_002058"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDX6520421.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            12676..13833
                     /locus_tag="RPM73_002059"
     CDS             12676..13833
                     /locus_tag="RPM73_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDX6520422.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13975..15015
                     /gene="galS"
                     /locus_tag="RPM73_002060"
     CDS             13975..15015
                     /gene="galS"
                     /locus_tag="RPM73_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDX6520423.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            15295..16293
                     /gene="mglB"
                     /locus_tag="RPM73_002061"
     CDS             15295..16293
                     /gene="mglB"
                     /locus_tag="RPM73_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDX6520424.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            16354..17874
                     /gene="mglA"
                     /locus_tag="RPM73_002062"
     CDS             16354..17874
                     /gene="mglA"
                     /locus_tag="RPM73_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDX6520425.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17890..18900
                     /gene="mglC"
                     /locus_tag="RPM73_002063"
     CDS             17890..18900
                     /gene="mglC"
                     /locus_tag="RPM73_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDX6520426.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18971..20206)
                     /gene="preA"
                     /locus_tag="RPM73_002064"
     CDS             complement(18971..20206)
                     /gene="preA"
                     /locus_tag="RPM73_002064"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDX6520427.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGITAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELCDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(20200..21438)
                     /gene="preT"
                     /locus_tag="RPM73_002065"
     CDS             complement(20200..21438)
                     /gene="preT"
                     /locus_tag="RPM73_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDX6520428.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         5207 a         5348 c         5660 g         5472 t
ORIGIN      
        1 tcgtaaaaat agcgtgtcgt ttaataaccg cgggcggtaa tgtttcatag ggagcgcaag
       61 gcaccaacac tcttcaatag cctgcaccgc acgcggttca tggtctttgt cgattaactc
      121 aatctcgcca ataaaacagg gggcggcaaa gaaatcgatc agcgacacgc gaccattagc
      181 tagcgtggcg taaagcctgg cgcggcctcg cgtcaggtaa aacagccagg acggctgttg
      241 cccttcctgc acaatgtagt cacgcgctaa aaaatgaaac agccgcgtat cggctgaaac
      301 atccgttaaa aagcaatcct taaacgcgga ttcactcatc aattgctgct taagatcatt
      361 attgtggatt tctttcacga tgtttatccg ctatgagatt tctcatattt agcatacgta
      421 cgcggttggt aagattcaat catcggcccg gtcgaatttt ctttgctgta gaaaaaataa
      481 cgcaactgga aacagaggaa ataaacaatg gaaaagagaa aaattattct ggattgtgat
      541 ccgggtcatg atgatgctat tgctataatg atggcggcga aacatccggc aatagattta
      601 ttaggcatca ctattgtagc gggtaatcag acgcttgata aaacattaat taatggcctg
      661 aatgtttgcc agaaactgga gattaatgtt ccggtttatg cggggatgcc gcaacccatt
      721 atgcgtcaac aaatcgttgc cgataatatt cacggtgaaa ccggactgga tggcccggta
      781 ttcgagccgc tgacccgcca ggcagaaagt acccacgcgg tgaaatacat cattgatacc
      841 ctgatggcaa gcgatggcga tatcactctg gtgccggttg gtccgctttc aaatatcgcg
      901 gtggcaatgc gtatgcaacc cgcgatcctg cccaaaatcc gtgaaattgt gctaatgggc
      961 ggcgcttacg gtacaggcaa cttcacgcca tctgccgagt tcaacatctt tgctgacccg
     1021 gaagcagccc gcgtggtgtt cacctctggc gttccattag tgatgatggg cctcgatctc
     1081 accaaccaga ccgtttgcac cccggacgtg attgctcgga tggaaagggc aggcggccct
     1141 gccggagagc tgttcagcga catcatgaac ttcactctca aaacgcagtt cgaaaactac
     1201 ggcctggctg gcggcccggt gcacgacgcc acctgcatcg gttatctgat taaccctgat
     1261 ggcattaaaa cccaggagat gtacgtcgaa gtggacgtca acagtggccc ttgctatggg
     1321 cgcaccgtct gcgacgagct gggcgttctt ggcaagcccg ccaataccaa agtcggcatc
     1381 actattgata ccgactggtt ctggggctta gtcgaagagt gcgtgcgcgg ctacatcaaa
     1441 acccattaag cgttgataac aagccgggcc gatagcgcaa gatgcaggcc cggctatttc
     1501 tgatgggcag aaagccgtcg cccaatatga catgaagaga taatcactat ggatgtcatg
     1561 agaagtgttc tgggaatggt ggtattgctg gcaattgcat ttttgctgtc agtaaacaag
     1621 aagaagatca gcctgcgtac cgttggcgcg gcgttagtgt tacaggttgt gattggcggc
     1681 attatgcttt ggttaccgca agggcgttgg gtcgctgaaa aagtcgcttt tggcgtgcat
     1741 aaagtgatgg cgtacagcga cgcgggtagc gcatttatct tcggttcgct ggtcgggccg
     1801 aaaatggaca cgctgtttga tggcgcagga tttatctttg gtttcagagt attaccggca
     1861 attatcttcg ttacagcact ggtgagtatt ctctactaca tcggtgtgat ggggatttta
     1921 attcgcattc tcggcggtat cttccagaaa gcgttaaata tcagcaagat cgagtcattc
     1981 gtcgcggtca ctaccatttt cctcgggcaa aacgaaattc cggcaatcgt caaacccttt
     2041 atcgatcgtc tgaatcgcaa tgaattattt acagcgattt gtagtggcat ggcctcgatt
     2101 gctggttcga caatgattgg ttacgccgcc ctgggcgtac ctgtggaata tttgctggcg
     2161 gcatcgttaa tggcgatccc aggcgggatc ttgtttgccc gcctgttaag cccggctacg
     2221 gaatcttcgc aggtttcctt taataacctc tctttcaccg aaacaccgcc aaaaagcatt
     2281 attgaagccg ctgcgacagg ggcaatgacc gggctgaaaa tcgccgccgg tgtagcgaca
     2341 gttgttatgg catttgtcgc catcattgcg ttaattaatg gtattatcgg cggcgttggc
     2401 ggctggtttg gttttgcaca tgcctcgctg gagtccattt taggttacct gttggcccca
     2461 ttggcgtggg tgatgggggt tgactggagt gatgcaaatc ttgccgggag tttgattggg
     2521 cagaagctgg cgatcaatga atttgtcgct tatctcaatt tctcgccata tctgcaaacg
     2581 ggtggcactc tggatgctaa aaccgtggcg attatttctt tcgcgttgtg cggtttcgct
     2641 aactttggtt ctatcggggt ggtggtgggg gcgttttctg cggttgcgcc acaccgtgcg
     2701 ccggaaatcg cccaacttgg tttacgcgcg ctggcggcgg cgacactttc taacctgatg
     2761 agtgccacca ttgccgggtt ctttattggt ttagcgtagt gtgcgattta tgagccggat
     2821 aagatgcggt caagcgtcga atccggcaac attgttacac cattggcact aatgaaagcg
     2881 cgttatcggc agacagggta gggttgttta agctgccgct ggcgcgcgaa attgccgcgc
     2941 aggctaccgc aaaacgggca ctgtcgcgga aactgtatcc ttcaagaaag ctatatacca
     3001 ggcccgccat aaaaccatcg tcagcaccaa aactgtctac tgtcgtatgc gctggcgcgg
     3061 tcagcaaaaa ttgttctcca tccttatcgc tgcaaaatac agattcatct ttcagataaa
     3121 caaaaatgcg tttaacacct tgttgatgca gcgaatttac agctctgatg cgatcgttgt
     3181 cgtctttaat cggttgcccc cacaaaattt ccagttcatt ttgtgttggt ttcagagtgt
     3241 ggatccggct gtaccagttt tttactttgt ttgctttaaa ttcggaaacg gtatcgacaa
     3301 acatcggaat ttcatcagca atggtaaaga cccactccag ggcttcaggt gtgagattac
     3361 aatcagccag caccacacca gcatggcgaa tcaagtcccg tgaagtattg agcagttgag
     3421 gcgttagctg ttgcagaata tgtgtgtcat tgattgccag gatagtttct tcctgcttat
     3481 tagcgatagc gagataagtt gcagtgctgt gtccatgcag acgaatgcag tttgaaacgt
     3541 tcacgcccgc gcggcgcgtt tcttccagta atgtctcgcc gtaaaaatcg ttgccaatag
     3601 ctgatattaa atgcacatcc cgaccaagca gagccaggtt atgcgcgata ttacgtccga
     3661 cgccgcctgc ggaacagtga acagaacctg gatgagaggc tgcttgcggg taacggatat
     3721 ccgccattcc gcgaatatcc atattgattg cacccacaac cacacagtaa tcctgctcgg
     3781 taaggatata tcctttgcct ttaatcaaac ccttgcgcat caaatccata atatgagcgg
     3841 caacacgtga gcggctgatt tgcagaatat cggcgatttc attctgttga atcagcgggt
     3901 tacgccgtaa aattttaaga atctgctttt ccctgtcgtt catcgatcag gccaccgctt
     3961 tttcagtttg ttgcgctttc agccaggcga tctcttctgc ccagatatcc gggttgatgg
     4021 tttcaaggat cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag cggaacgcat
     4081 catgaccgat attgccttca ccgaggctat gatggcggtc aacgcggctg ccaaaggtgc
     4141 ttttcgcatc gttaaggtgc atcccgcgca gatacttaaa gccaacaata cgggcaaaat
     4201 ccgcgaacgt tttctcgcat tcggctggag tacgcaaatc atacccggca gcgaaagcat
     4261 ggcaggtatc aatgcagacg ccgacacggg atttatcttc cacgccgtcg ataatcgccg
     4321 cgagatgttc gaatttaaac cctaagttac tgccctgacc ggcggtgttt tcaatcaccg
     4381 ctgttacacc ttgagtttta tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc
     4441 aatcctcttc tgaaatctgc atcagatggc tgccagggtg gaagttgagc aaagaaagcc
     4501 ccagttgttc gcaacgctgc atttcatcta taaaggcatc gcgcgatttt tccagagctt
     4561 cagtgaccgg atgtccgagg ttaatcagat agctgtcgtg gggaagaatt tgcgccgatg
     4621 tgtagtgata tttttcacag gccgctttga attcatcgat ggtttgcgtc gtgagcggtg
     4681 cggcacgcca ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg
     4741 cggcgcgaat tgcggcattt gccagaccgc cagcagcact aacgtgcgct ccaatgtatt
     4801 tcatgcgagg actcctgtta aacccgctgg aggaaaacgg taatgatagc gggttaacag
     4861 gagcaagatg tagtggttta tgcgatgacg ctttgaatca catagttaat cgcaccacca
     4921 ccaacaatca gccaggcaaa cagtaccagt gccatcagca gaggtttcgc cccggctttt
     4981 ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc cagcaggaag
     5041 gtatccagcg ttaccagcat gttcaccacg ctctgcggta acaggtggaa cgagttaaag
     5101 atggcaacta cgatgaacaa gatggcaaac cacggaatag tgattttgct tttctcgccg
     5161 ctgttcgccc cagacagctg tttaacacgc gccgccagca ggatgaggaa cggagccagc
     5221 atcatcacgc gcagcatttt ggaaataact gctgcgtttt ccgcatccgg gctgatggca
     5281 tgacctgccg ccaccacctg cgccacttcg tgcacagtag aaccaatgta gataccgaaa
     5341 gtttccggac taaaccattg agacatcagc ggatatatcg ccgggtagag gaaaatcgcg
     5401 acggtcccga agataacaac ggttgcaaca gccacggtta ctttactggc ttccgctttc
     5461 actaccggct cagtcgccag taccgcggca gcaccacaga tactgctacc ggcaccgatc
     5521 aaccagctgg tgtgcttatc cagaccaaac actttctgcc ccaggaagca agccagcagg
     5581 aaggtactgg acagcgtcaa cacgtcaatg atgatcccac tgataccgac atcggcaatt
     5641 tgcgagaacg tcagacggaa gccataaaga atgataccca gacgtaataa atattgcttg
     5701 gcaaacagca caccaccgtc acagcttttc cagatgtgcg gatagatggt gttgcctaaa
     5761 accatcccca acaagattgc gagggtgagg gcactaaacc cggcacccgc aaccgcggga
     5821 atggaaccac cccacagggc gaccccggtg ataactgcac tcagggctaa ccccggaata
     5881 aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac cctctccttt
     5941 aaccgggcat aaggttacga ctaactggtt taaaaataaa attgattata tatttataat
     6001 taatctttat aagtggtaag cgactatgca catcaccctc cggcagttgg aagtttttgc
     6061 agaagtattg aaaagtggat caaccaccca ggcgtcggtg atgctggcgt tgtcgcaatc
     6121 agcagtgagc gcagccttga ccgacctgga agggcagctt ggcgtgcaac tgtttgatcg
     6181 cgtggggaaa agactggttg ttaatgaaca cgggcggctg ctctatccgc gtgcgttggc
     6241 attgcttgaa caggcggttg aaatcgaaca actgtttcgc gaagacaacg gcgcgattcg
     6301 tatctatgcc agtagtacca tcggtaacta cattctgcct gcagttatcg cccgttatcg
     6361 ccatgattat ccgcagttgc cgattgaact tagcgttggg aacagccagg acgtgatgca
     6421 agcggtgctg gatttccgcg ttgatattgg ttttattgaa ggaccgtgcc acagcactga
     6481 aattatttct gaaccgtggc tggaagacga gctggtggtt ttcgccgcgc cgacttcgcc
     6541 gttggcccgt ggtccggtca ccttagagca gctggccgct gcgccgtgga tcctgcgtga
     6601 acgcggttcc ggcacgcggg agattgtcga ttatctattg ctgtcacatt taccgaagtt
     6661 tgagatggcg atggaattag gtaactccga ggcaatcaaa catgcggtgc gtcatgggtt
     6721 gggaattagt tgcctgtcgc gacgggtgat tgaagatcaa ttgcaggcag gcacattaag
     6781 tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg tggcgtatac atcatcggca
     6841 aaaacacctt tccaacgcgc tacggcgctt tctggactat tgcgatcccg cgaatgtacc
     6901 gcgttaagtt gctgtacaag aacatgctgg tgctgtgtcg atttcgtgac gcagcgccct
     6961 cagcatgcat tcgccagaaa agagattggc tgctttactt ataatccctg ggcgatcatg
     7021 aaggtgtctt ataaccgtgt atttctgccg gaaggattgc caatcgtctg ctacaatcgc
     7081 gcctcatttt taagatggat agcatttttg tatggtttcc gaaactaaaa ccacagaagc
     7141 gccgggctta cgccgtgaat taaaggcgcg tcacctgacg atgattgcca ttggcggttc
     7201 catcggtaca ggtctttttg ttgcctctgg cgcaacgatt tctcaggcag gtccgggcgg
     7261 ggcattgctc tcgtatatgc tgattggcct gatggtttac ttcctgatga ccagtctcgg
     7321 tgaactggct gcatatatgc cggtttccgg ttcgtttgcc acttacggtc agaactatgt
     7381 tgaagaaggc tttggcttcg cgctgggctg gaactactgg tacaactggg cggtgactat
     7441 cgccgttgac ctggttgcag ctcagctggt catgagctgg tggttcccgg atacaccggg
     7501 ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg ctgaactaca tctcagttcg
     7561 tggctttggt gaagcggaat actggttctc actgatcaaa gtcacgacag ttattgtctt
     7621 tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa ggcgcgcagc ctgcgggctg
     7681 gagcaactgg acaatcggcg aagcgccgtt tgctggtggt tttgcggcga tgatcggcgt
     7741 agctatgatt gtcggcttct ctttccaggg aaccgagctg atcggtattg ctgcaggcga
     7801 gtccgaagat ccggcgaaaa acattccacg cgcggtacgt caggtgttct ggcgaatcct
     7861 gttgttctat gtgttcgcga tcctgattat cagcctgatt attccgtaca ccgatccgag
     7921 cctgctgcgt aacgatgtta aagacatcag cgttagtccg ttcaccctgg tgttccagca
     7981 cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt attctgacgg cggtgctgtc
     8041 agcgggtaac tccggtatgt atgcgtctac tcgtatgctg tacaccctgg cgtgtgacgg
     8101 taaagcgccg cgcattttcg ctaaactgtc gcgtggtggc gtgccgcgta atgcgctgta
     8161 tgcgacgacg gtgattgccg gtctgtgctt cctgacctcc atgtttggca accagacggt
     8221 atacctgtgg ctgctgaaca cctccgggat gacgggtttt atcgcctggc tggggattgc
     8281 cattagccac tatcgcttcc gtcgcggtta cgtattgcag ggacacgaca ttaacgatct
     8341 gccgtaccgt tcaggtttct tcccactggg gccgatcttc gcattcattc tgtgtctgat
     8401 tatcactttg ggccagaact acgaagcgtt cctgaaagat accattgact ggggcggcgt
     8461 agcggcaacg tatattggta tcccgctgtt cctgattatt tggttcggct acaagctgat
     8521 taaaggaact cacttcgtac gctacagcga aatgaagttc ccgcagaacg ataagaaata
     8581 agtttcctcc cttccttgct aagccctctc aaccgagagg gctttttcaa ttccatttcc
     8641 ctgacaaatc atgcggatat aaaatttaac atttggattg ataattgtta tcgtttgcat
     8701 tatcgttacg ccgcaatcaa aaaaggctga caaatcagag gctgttccgg ctttctggga
     8761 tgatcacctg catacaaata agtccaccgc gatgctgccg tacgcaaggg gacgtgaaga
     8821 agatgtgagc gataacccat tttattttcg tagttacctc atggagatat ggaatgttta
     8881 ggttgaaccc tttcgtacgg gtcgggctgt gtttgtccgc tatttcttgt gcatggcctg
     8941 tgttagcggt cgatgatgat ggcgaaacga tggttgtcac tgcatcttcc gttgaacaaa
     9001 acctcaaaga tgcacccgcc agtatcagcg tcattaccca ggaagacctg cagcgaaaac
     9061 cggtacagaa tctgaaggat gtcctcaaag aagtgcctgg cgtacaactg acgaacgaag
     9121 gggataaccg taagggcgtt agtattcgtg gtctggacag cagctacacc ctgattctcg
     9181 tcgacggtaa acgcgttaac tcccgcaatg ccgtcttccg ccacaatgat ttcgatctga
     9241 actggatccc ggtcgattcc atcgaacgta ttgaagtggt tcgtggcccg atgtcgtcgc
     9301 tatacggctc cgatgcgctc ggcggtgtag tgaatatcat caccaaaaaa atcggtcaga
     9361 aatggtcggg taccgttacc gtcgatacca ccattcagga acatcgcgat cgcggtgata
     9421 cctataacgg tcagttcttt accagtggac cattaattga tggtgtgctg ggaatgaaag
     9481 cttacggcag cctggcaaaa cgtgaaaagg atgacccgca aaactcaacg accaccgata
     9541 ccggagaaac gccgcgtatt gaaggattct ccagccgcga cggcaatgtc gaatttgcct
     9601 ggacaccgaa tcaaaatcac gattttactg ccggatacgg tttcgaccgt caggatcgtg
     9661 attccgactc gctggacaaa aaccgcctgg aacgccagaa ctactccgtc agccataatg
     9721 ggcgttggga ttacggcacc agcgaactga aatactacgg tgagaaagtc gagaacaaaa
     9781 accctggcaa cagcagcccg ataacttccg aaagcaatac ggtcgacggc aaatacacgt
     9841 tgccgctgac ggcgattaat cagtttctca cggttggcgg tgaatggcgt cacgacaaac
     9901 ttagcgatgc ggtgaacctg accgggggaa ccagctccaa aacgtctgcc agccagtacg
     9961 cgctgtttgt ggaagatgaa tggcggatct tcgagccgct ggcgctgacg accggcgtgc
    10021 gtatggacga tcacgaaacc tacggtgaac actggagtcc gcgtgcctac ctggtttata
    10081 acgccaccga caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa gcgccttcgc
    10141 tgttgcaact tagccctgac tggacgagca attcctgccg tggcgcatgt aagattgtgg
    10201 gtagcccgga tctgaaacca gaaaccagcg aaagttggga gctggggctt tactacatgg
    10261 gtgaagaagg ctggctggaa ggggttgaat ccagcgttac cgttttccgt aacgatgtga
    10321 aagatcgtat cagcatcagc cgtacgtctg acgtcaacgc tgcaccgggc taccaaaact
    10381 ttgttggttt tgagacgggc gctaacggac ggcgcatacc ggtatttagc tactacaatg
    10441 ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat
    10501 ggaaactgtc gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca
    10561 aaccgctatc cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg
    10621 cgctggaaga ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgcgccgaca
    10681 gcgcgacggc taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg
    10741 tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc
    10801 gtgacgacta cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct
    10861 tctgatgaga agatgcccgg cgaaccgggc ggactttcac ttcagtaaat actgcgcatg
    10921 gaagcgcagg tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg
    10981 ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca gtaccgcagg
    11041 ttgcaactgg tcggcaagaa actgatcatt atcgccctga tcgataagcg tcgggatcgc
    11101 atcctgcgcg ttactggcat acatcagcgc gcaactgtcc cattccagcc atgcattttt
    11161 gtcctcacct aaatagctgc taaacgcttt gattccccac gggacgctgc acggattcac
    11221 aattggcgca aaggccgaaa cgctggtgta tttacccgga tttttcagcg ccataatcag
    11281 cgcaccgtga ccacccattg agtgaccgct aatggcgcag cggtcgctga cattaaattg
    11341 cgactgaacc agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc
    11401 ccacggcggt tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc
    11461 gtcgttggca accttttcgc cgcgcgggct ggtgtctggc atcaccagta caatccccag
    11521 ttctgccgcc acgcgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa
    11581 tccggaaagc cagtacagca ctggtggcgg ggtgtgatca cgagggggtg ggagaaagat
    11641 actgaacgtc atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc
    11701 ttcaaaacag cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca
    11761 atttcttcat cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca
    11821 taacgatgtt ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg
    11881 aatactggat tatgtgcgcc gcctcacgca caataatcag gctgtaaatc agcttaataa
    11941 ctttgccccc acgcagggcg gaggcgtcac acctgcagga gaaatcataa atgccatcac
    12001 tcagtaaaga agcggccctg gttcatgaag cgttagttgc gcgaggactg gaaacaccgc
    12061 tgcgcccgcc cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata
    12121 tgaccgaaat catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc
    12181 cgcatcgcat cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaact
    12241 tcccgaaaat caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg
    12301 atatcactct gaccagtacc tgtgaacacc attttgttac catcgatggc aaagcgacgg
    12361 tggcttatat cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt atcgtgcagt
    12421 tctttgccca gcgtccgcag gtgcaggaac gtctgacgca gcaaattctt attgcgctac
    12481 aaacgctgct gggcaccaat aacgtggctg tctcgatcga cgcggtgcat tactgcgtga
    12541 aggcgcgtgg catccgcgat gcaaccagtg ccacaacaac gacctctctt ggtggattgt
    12601 tcaaatccag tcagaatacg cgccacgagt ttctgcgcgc tgtgcgtcat cacaactgat
    12661 taaaaggcag gaaccatgga gcgcaacgtc acgctcgatt ttgttcgcgg cgtcgccatt
    12721 ctggggatcc tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat
    12781 cccgcctggt acggcgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc
    12841 atcggccagg tgaaattcct cacgcttttt gcgctgctgt ttggtgcggg cctgcaaatg
    12901 ttgctgcccc gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttattgggc
    12961 tttattcacg gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggc
    13021 ttaatctgct ggcggctggt gcgcgatgcg ccatcggtaa aaagcctgtt taataccggc
    13081 gtcatgcttt atctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    13141 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    13201 cttcacggcg gcgttgatgc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    13261 gcactgggcg cacagtatgg ctggcaactg gctgggatga tgctcattgg tgcggcgctg
    13321 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    13381 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    13441 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    13501 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt aagccgcttt
    13561 aagctggtgc tggcaatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    13621 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgatcgcctg
    13681 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    13741 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    13801 gccggaacgg caatatctaa aacatcaaga taacgatctg gatcacattc gttaacaaaa
    13861 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    13921 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    13981 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    14041 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    14101 ctggattatc ggccaaatgc caatgcccag gcgctggcaa ctcaggttag cgataccatt
    14161 ggcgtggtgg tgatggacgt ttctgatgct tttttcggcg cgctggtaaa agcggtggat
    14221 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    14281 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    14341 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    14401 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    14461 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    14521 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    14581 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    14641 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    14701 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    14761 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    14821 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg cgaaattagc caccgaactg
    14881 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    14941 ttagtgcgtc gccattctgt cgcaacacgc cagaatgcgg cgacgatcac taactcaaca
    15001 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    15061 tagctatgat tgcaccgttt taacgttgta acccgtatgt aacagtgaat aatcactttc
    15121 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    15181 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    15241 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    15301 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    15361 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    15421 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    15481 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    15541 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    15601 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    15661 gatagctacg acaaagccta ctacgttggc actgattcca aagagtccgg cattattcag
    15721 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    15781 cagattcaat tcgtattgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    15841 acttacgtga ttaaagagct gaacgacaaa ggcattaaaa ctgaacagtt acagttagat
    15901 accgctatgt gggataccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    15961 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    16021 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    16081 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    16141 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    16201 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    16261 aaagacaacc tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    16321 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    16381 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    16441 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    16501 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    16561 attttattcc agggtaaaga gatcgatttc cattctgcca aagaagcact ggaaaatggt
    16621 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    16681 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    16741 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    16801 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    16861 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    16921 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    16981 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    17041 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    17101 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    17161 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    17221 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    17281 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    17341 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    17401 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    17461 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    17521 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    17581 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    17641 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    17701 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    17761 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    17821 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    17881 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    17941 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    18001 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    18061 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    18121 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    18181 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    18241 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    18301 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    18361 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    18421 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    18481 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    18541 gaagcggcaa aagtatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    18601 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    18661 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    18721 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    18781 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    18841 ggggcgatta ttatcttcgc cgtagcgttg gattcactga aatacgcgcg taagaagtga
    18901 tgtgattgtg gatattgccg gatgcgctgc atccggcaat cggtgccaaa tgccggatgc
    18961 gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc tttcttaaac ttcacttccc
    19021 cgagctcaat acaacccacc gggcagacgt gaccacaaag cagacaaccc acacatttct
    19081 cggtattaca atgcggtgtg cgggttttct cgctccattc catcgcctgg tgaccgccgt
    19141 cgtaacagga aatataacag cgtccacagc caacacattt atcaagattg atacggggat
    19201 agacaatata actgcggtct aaatcttccg ccgggacaat attgttattc gccagaccta
    19261 ccatctcctg cagcgaatca aatccctgat cggcgagata atgcgacaac ccgcttgcca
    19321 tatcttccac tatccgatac ccgtactgca tgatgccggt ggtcacctgt aacgttgctg
    19381 cgccgagcaa taaaaactca gccgcatcct cccaggtttc aatgccgccg ataccgctga
    19441 ttgggaaatc gcacagttct ggatgggtgc gcatctgctg aatgaagcgc agggcgatcg
    19501 gttttaccgc tttaccggaa tatccggaaa tactcgattt tccgttaacg atcggcatac
    19561 cgattttctg attaagatcg atattggtga tggatttaac ggtgttaatc gccgtaatgc
    19621 catctgcgcc gccgcgcttc gccgccagcg ccacttcgca catatcaccg atattcggcg
    19681 tcatcttcgc cagcattggc agcgtggaac cccgtttcac tgcccgacaa tatttttcta
    19741 ccagctccgg gctttgcccg acatcgctac ccatcgcatg agaagtcatt tgcggacagg
    19801 agaagttaca ctcgatcata tccgcgccag cttcttgcac caggcgcgcc agctcctccc
    19861 attgctgctc attttccccc atgatcgaag cgatcaatac tttgtccggg taatcttcct
    19921 tcagctgacg cagggcggcc agattctctt ccaacggatg ttcagcaatc tgctccatat
    19981 ttttgaagcc gataaaaccg gtatcttctt tcaccagatg atcaaaacgc ggcgagactt
    20041 cgttggcgat aaaaaagccg atcgttttaa acaccacgcc gccccaacct gtgtcgtagg
    20101 ctttggcaca catctcatag cagttgccta caggcgaaga agagaggcag aacgggttgg
    20161 gaaacttcac gccgcaaaaa gtaatcgaaa gatctttcgt taacatgagc aagctccctc
    20221 taaatagtga tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt
    20281 tttgtcaccc tcaacaatat cgccagcagc aaagacttgc ggatcgcggg tctgataatt
    20341 ttgtgttttg atggtgttac gctgcggctc taactccgca aaggcatcca gtctggcatg
    20401 ctgaccgacg gcgagaataa ttttatctgc cgccatcgtc agttcgcccg gtaaccgtac
    20461 atgcttaaac gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga
    20521 aacgcccagc tcccgggcgc tggcaaactc tttttcgctt gccggaaact catctaactc
    20581 ttcacgcgct acgcaggtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc
    20641 catcgcgaca tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg
    20701 cgcctgtcgt acacgttgca ggaagtcgac ggcaatctca acgtcactat gctcaaacag
    20761 cggtagcccg gaaccgcttg ataaccccac ggtgaccagt accgcgcggt tttctgcttt
    20821 cagctgctca agggtgagtg tgttaccgac ttcgttgttg cacttaatgg tcacgcccat
    20881 tttttcaata cgggcgatct ctgcatccag cactgattgc ggtaaccgga attgcggaat
    20941 accgttacgc aaccagccac cggggtgcgc ttctttctca taaatcgtga cgtcataacc
    21001 ctggtttgtc agtgttacac tggcctgcaa tccggcagga cctgcgccaa taatcgcgac
    21061 tttgccgagc gttttagtac cgggctgata aatttccatc ccagtttgtt gttcaaaatc
    21121 agtaacaaaa cgctgtaagc gaccgatatc aatgggagta tcaacaccgg cacgggtaca
    21181 accgctttga cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt
    21241 attttcgcga attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa
    21301 tttccccgga tcggtctgtg ccgggcaagc ctgactacag ggagcgtcat gacataataa
    21361 acagcgagag gcttctttaa tcgccagtaa aggcgtaaaa gccggagtga gttcatccag
    21421 ataattttgt tgcggcatgt gatcttcctt aatgctatga gtaattaagg cgaacgctaa
    21481 atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gttcgctcac cccagtcact
    21541 tacttatgta agctcctggg gactcactca cttgtcgcct tcctgcaact cgaattattt
    21601 agagtataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt tatactgtga
    21661 aaggattcac aaatagctgc ggttcag
//
LOCUS       DAPPNJ010000067        21379 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000067 DAPPNJ010000000
VERSION     DAPPNJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.067"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(194..1222)
                     /gene="epmB"
                     /locus_tag="RPM73_002066"
     CDS             complement(194..1222)
                     /gene="epmB"
                     /locus_tag="RPM73_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDX6520429.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1264..1830
                     /gene="efp"
                     /locus_tag="RPM73_002067"
     CDS             1264..1830
                     /gene="efp"
                     /locus_tag="RPM73_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDX6520430.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1882..2007
                     /gene="ecnA"
                     /locus_tag="RPM73_002068"
     CDS             1882..2007
                     /gene="ecnA"
                     /locus_tag="RPM73_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDX6520431.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2118..2264
                     /gene="ecnB"
                     /locus_tag="RPM73_002069"
     CDS             2118..2264
                     /gene="ecnB"
                     /locus_tag="RPM73_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDX6520432.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2439..2756
                     /gene="sugE"
                     /locus_tag="RPM73_002070"
     CDS             2439..2756
                     /gene="sugE"
                     /locus_tag="RPM73_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDX6520433.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2753..3286)
                     /gene="blc"
                     /locus_tag="RPM73_002071"
     CDS             complement(2753..3286)
                     /gene="blc"
                     /locus_tag="RPM73_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDX6520434.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3375..4508)
                     /locus_tag="RPM73_002072"
     CDS             complement(3375..4508)
                     /locus_tag="RPM73_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDX6520435.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4571..4930)
                     /gene="frdD"
                     /locus_tag="RPM73_002073"
     CDS             complement(4571..4930)
                     /gene="frdD"
                     /locus_tag="RPM73_002073"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDX6520436.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(4941..5336)
                     /gene="frdC"
                     /locus_tag="RPM73_002074"
     CDS             complement(4941..5336)
                     /gene="frdC"
                     /locus_tag="RPM73_002074"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDX6520437.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5347..6081)
                     /gene="frdB"
                     /locus_tag="RPM73_002075"
     CDS             complement(5347..6081)
                     /gene="frdB"
                     /locus_tag="RPM73_002075"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDX6520438.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6074..7882)
                     /gene="frdA"
                     /locus_tag="RPM73_002076"
     CDS             complement(6074..7882)
                     /gene="frdA"
                     /locus_tag="RPM73_002076"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDX6520439.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8207..9184
                     /gene="epmA"
                     /locus_tag="RPM73_002077"
     CDS             8207..9184
                     /gene="epmA"
                     /locus_tag="RPM73_002077"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDX6520440.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9403..10905
                     /gene="yjeM"
                     /locus_tag="RPM73_002078"
     CDS             9403..10905
                     /gene="yjeM"
                     /locus_tag="RPM73_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDX6520441.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            10957..11271
                     /locus_tag="RPM73_002079"
     CDS             10957..11271
                     /locus_tag="RPM73_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520442.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            11268..11582
                     /locus_tag="RPM73_002080"
     CDS             11268..11582
                     /locus_tag="RPM73_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDX6520443.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11611..14934)
                     /gene="mscM"
                     /locus_tag="RPM73_002081"
     CDS             complement(11611..14934)
                     /gene="mscM"
                     /locus_tag="RPM73_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDX6520444.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14956..15924)
                     /gene="psd"
                     /locus_tag="RPM73_002082"
     CDS             complement(14956..15924)
                     /gene="psd"
                     /locus_tag="RPM73_002082"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDX6520445.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGEKDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16021..17073)
                     /gene="rsgA"
                     /locus_tag="RPM73_002083"
     CDS             complement(16021..17073)
                     /gene="rsgA"
                     /locus_tag="RPM73_002083"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDX6520446.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17168..17713
                     /gene="orn"
                     /locus_tag="RPM73_002084"
     CDS             17168..17713
                     /gene="orn"
                     /locus_tag="RPM73_002084"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDX6520447.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17924..17999
                     /locus_tag="RPM73_002085"
     tRNA            17924..17999
                     /locus_tag="RPM73_002085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17957..17959,aa:Gly,seq:gcc)
     gene            18054..18127
                     /locus_tag="RPM73_002086"
     tRNA            18054..18127
                     /locus_tag="RPM73_002086"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18086..18088,aa:Cys,seq:gca)
     gene            18140..18226
                     /locus_tag="RPM73_002087"
     tRNA            18140..18226
                     /locus_tag="RPM73_002087"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18174..18176,aa:Leu,seq:taa)
     gene            18422..19087
                     /gene="yecA"
                     /locus_tag="RPM73_002088"
     CDS             18422..19087
                     /gene="yecA"
                     /locus_tag="RPM73_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDX6520448.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(19149..20360)
                     /gene="tyrP"
                     /locus_tag="RPM73_002089"
     CDS             complement(19149..20360)
                     /gene="tyrP"
                     /locus_tag="RPM73_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDX6520449.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            20551..20790
                     /locus_tag="RPM73_002090"
     CDS             20551..20790
                     /locus_tag="RPM73_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDX6520450.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            complement(20828..21325)
                     /gene="ftnA"
                     /locus_tag="RPM73_002091"
     CDS             complement(20828..21325)
                     /gene="ftnA"
                     /locus_tag="RPM73_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDX6520451.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
BASE COUNT         5051 a         5451 c         5606 g         5271 t
ORIGIN      
        1 atgcaacggg caaagattaa atttcccagt ttaatgacag gttgctgtac ttgcgtttgt
       61 tgctcttgcc ggatgcggcg taaacgcctt atccggccta cagttcattg caaatccatt
      121 caatttcaat gcacaatgta ggcctgataa gcgtagcgca tcaggctaat gtttaacttc
      181 cctgtttaat cagttactgc tggcgtagct ggagatccag cggcgttttg ctgggttcgc
      241 cgccaatttc tcgcgccagt ttcggcacca gatatcccga caccaatgtc agcaactcac
      301 gcataatctg ccgtgcttcg tcatcactca ccataaaatg cgccgcgccc tgtactttat
      361 ctagcacatg caggtaatac ggcatcacgc cggcatcgaa caacgcatta ctcaggtttg
      421 ccaacgtttg tgcgttatca ttcacaccac gtaacagaac gctctggtta agcagggtga
      481 caccgacacg gcgcaattta gccatcgcct gacggaacgt ttcatctacc tcattggcat
      541 gattgatgtg attcaccagc aagatttgca gcgtagaacg ggcaaagcgt tcaaccagcg
      601 cctcggtgat acgtgccggg atcacaatcg gcagacggct gtgaatccgc agacgtttga
      661 tatgcgggat ggcttccagt tgtgtgagca accagtccag ctcgtgatct ttcgccatca
      721 gcggatcgcc gccggagaaa atcatctcgt ccagttccgg atgcgcagca acatactcaa
      781 gagcagtctg ccagttacgc ttgttgccct gattttcggc ataggggaag tggcgacgga
      841 agcaatagcg gcaatttacc gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt
      901 atttatgcaa caaaccaggc actacgctgt gctgttcttc cagcgggtcg gtggagaatc
      961 cggacgcgac gacaaactca tcttgcgagg taagtacctg acgcaaaaga ggatcgtccg
     1021 gattgccttt ctccatgcga tcgataaatg agcggggcac acgcagggca aacagctttt
     1081 tggcgctgcg tccggccaac agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt
     1141 catcaggatc ggtcacaaca tcggcaagtt gcgttaacca atcttctctg gatggggtat
     1201 ttagggttac aatatgcgcc attttgtggc ttagctacca attaacaaat ttcagagggc
     1261 cttatggcaa cgtactatag caacgatttt cgtgctggtc ttaaaatcat gttagacggc
     1321 gaaccttacg cggttgaagc gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct
     1381 cgcgttaaac tgcgtcgtct gctgaccggt actcgcgtag aaaaaacctt caaatctact
     1441 gattccgctg aaggcgctga tgttgtcgat atgaacctga cttacctgta caacgacggt
     1501 gagttctggc acttcatgaa caacgaaact ttcgagcagc tgtctgctga tgcaaaagca
     1561 attggcgaca acgctaaatg gctgctggat caggcagagt gtatcgtaac tctgtggaat
     1621 ggtcagccga tctccgttac tccgccgaac ttcgttgaac tggaaatcgt tgataccgat
     1681 ccgggcctga aaggtgatac cgcaggtact ggcggcaaac cggctaccct gtctactggc
     1741 gctgtggtta aagttccgct gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc
     1801 tctggtgaat acgtctctcg cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac
     1861 catcacttat tcaggtcaga gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc
     1921 acgctgctca cgggctgtaa caccgctcgc ggtttcggcg aagacatcaa acatctcggc
     1981 aactccatct ctcgcgctgc cagctaattt ttcttctctt ccgaaaaatc atcagatttc
     2041 catcattttt ggcgatgttg tctattatta atttgctata ggcaaacata aataacatta
     2101 cctaaaagga agacgttatg gtgaagaaga caattgcagc gatcttttct gttctggtgc
     2161 tttcaacagt attaactgcc tgcaacacca cgcgtggcgt tggtgaagac atttctgatg
     2221 gcggtaacgc gatttctggt gcagcaacga aagcgcagca ataagcaata acggtacgac
     2281 agttatgtcg tgccgtttgt ttttttgcga tagtcacaaa ggtaatagtt gaaattcccc
     2341 tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg caaacgcctc
     2401 ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt atcttagtta
     2461 ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac ggctttagtc
     2521 gtttgacgcc aagtgttatt actgtgacgg cgatgatagt cagtatggcg ctacttgcct
     2581 gggcgatgaa atcgttaccg gtagggacgg cttatgccgt gtggacgggt attggcgcag
     2641 tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg atgcgcctgg
     2701 cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact cactaactac
     2761 cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc gcgactgcca
     2821 gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc cacaggtagt
     2881 cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca ataacgttat
     2941 aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt ggtgcgccgg
     3001 taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta tagcctttat
     3061 taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact ttttccagtc
     3121 cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga taacgtttgg
     3181 cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg caggcaacga
     3241 ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat gtttccttac
     3301 tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc cgaaaattcg
     3361 gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc gactctcgct
     3421 ggattgggat agtttttgtt agccagcatc acgataccca gctctttttc tggaataaac
     3481 gcgacatagc taccaaatcc gccggtcgcc cccgttttat gcacccatga tgcgcgtact
     3541 gcaggagttg ggggcgtaat cgcttttacg gggcgtgctg ccagtgcaat tttattgtca
     3601 ctgccgttaa cgatgatgtc aggatttacc ggccagtcca gcatttccca gcccagaccc
     3661 tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc ttgttgaagt
     3721 gttttgtcgt tgatatcacg gtgattcata ttgcttcgta cccagcaggc catatcttca
     3781 atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac atgaactgcc
     3841 ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac attaatccac
     3901 gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc aaaactcaaa
     3961 ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc atacagacgt
     4021 tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa gtcgcttgag
     4081 gatttcacct catccggcac ctgcaatggc aggccgccag tagtgtaggt tgcgagatgt
     4141 aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt tgttgtggga
     4201 tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc cgtaaatgtt
     4261 ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt tttggcgatg
     4321 tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc caccgccata
     4381 ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat atcgttgatt
     4441 tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca gagcgtcgtt
     4501 ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc ggatttacat
     4561 tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg atagcagcca
     4621 gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc atcgcgtggt
     4681 gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg aatacgcgac
     4741 caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca cccggaaaca
     4801 gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata attgcgctcc
     4861 acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc tttggatttg
     4921 gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac gatggtggca
     4981 accacagtta ccgcccagag acttttgata attggctctg gtcccatttt ttcgtctttt
     5041 acaatgatat tggctgcttt cggtgccagt tcaaaccagg ttttggtatg cagcagagct
     5101 gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa gtcgacgaat
     5161 cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag ttcaatgctg
     5221 aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata aaacggcaat
     5281 tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt cgtcatgttg
     5341 cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt ctactttgcc
     5401 ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc aatagcccac
     5461 gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct ccttcttacc
     5521 gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg gaccgatgaa
     5581 ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt tgatgcaacc
     5641 ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac cctgatccgc
     5701 ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga tgaagtgggt
     5761 catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa ccttcatacc
     5821 gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt taaccatcat
     5881 gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt ccggtgccag
     5941 gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat aaggcacttc
     6001 atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca cctcaatttt
     6061 caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc gccttatcgg
     6121 ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta atcttcacgt
     6181 cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg tgtttgagga
     6241 agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg ccgcgggact
     6301 ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg tggcccagtt
     6361 caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg cgcacgcgct
     6421 tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt tccggcgtac
     6481 ggtagatacc gcaaccttct tccatagcca gacccatttc gtcgcggatc ttcgcccagt
     6541 tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca gctgcctgcg
     6601 cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc gcttgttcac
     6661 cggccagacg gccgaagacc accagttccg ccagggagtt ggagcccaga cggtttgcac
     6721 cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg cgggtttcac
     6781 agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt accggaatcg
     6841 gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg aacggcagac
     6901 gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca tcgccacgcg
     6961 gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa actttgtcgc
     7021 gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc gggcccatgc
     7081 cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct tcaccgcggc
     7141 agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac tgaacgaatt
     7201 ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca ccggtaacga
     7261 tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc ataacgaccg
     7321 cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc aggccgcgaa
     7381 catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc tggatctgcg
     7441 ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc ttatcggcgg
     7501 cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg ctaccatccg
     7561 ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt gggcagtggt
     7621 ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct actgtatcgt
     7681 gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc ccttctgcag
     7741 caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt gcatttggat
     7801 ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct acaatggcaa
     7861 gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca cagccccgta
     7921 cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc tcctttatag
     7981 gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt tttttagtgc
     8041 gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca tcagatcgtg
     8101 ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc tcgccgtcgc
     8161 gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga gcgaaacggc
     8221 atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga ttatggcgga
     8281 gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt gtatgagcca
     8341 ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg gccccgggca
     8401 ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga aacgcctgct
     8461 ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg aagagatggg
     8521 gcgttatcac aaccctgagt tcactatgct tgagtggtat cgaccgcact atgatatgta
     8581 ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc cggcagcaga
     8641 aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc tctctgccga
     8701 caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agtaacgttg ctgataccga
     8761 agaagaccgc gacacgttgc tacaattgct gtttaccttt ggcgtagagc caaatattgg
     8821 taaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac tggcgcaaat
     8881 cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag gtattgagct
     8941 ggcgaatggt ttccatgaat taacggatgc ccgtgagcag caacaacgct ttgaacaaga
     9001 taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga atctgattga
     9061 agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg atcgtctggt
     9121 gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg ttgaccgggc
     9181 ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg ccattctgta
     9241 gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt tgatatcatc
     9301 caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca gtgcagttca
     9361 gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca cacgataaaa
     9421 aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg atttgccaat
     9481 agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat attttctgca
     9541 ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc ttatcgcaaa
     9601 gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt tgccttcatt
     9661 ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc cgcgaaagtt
     9721 tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg gcgtattgct
     9781 ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat tctggtcacc
     9841 gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg cggtattgca
     9901 gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt attaaatggt
     9961 gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta tcagtccggt
    10021 ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat tgaagcggtt
    10081 ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg tattgttttt
    10141 gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg tgtcagcaca
    10201 aactggcagc aggtattaag taatggttcc gttaacctcg gcaatattac ctatgtgctg
    10261 atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga agcgtcattg
    10321 tcgctgggtg tatggtttgc gcgtattacc ggactttcga tgttcctcgc ttataccggg
    10381 gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac gccgaaagcg
    10441 ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat cgccatgtgg
    10501 atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg cggtaccgca
    10561 tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct tccttacctg
    10621 ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag accatttgtg
    10681 attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact ggtggtgaca
    10741 tttgcgaacg tcttcaccat tattcagcct gtggttgaag ccggagactg ggacagcaca
    10801 ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat ttaccagaac
    10861 tattgcagca gagtggcaaa aaacccgcag tgggcggtgg aataacccga tgccctatcg
    10921 tctgcctgac gatagggcgt aaagacagga aatgttatgg atgacaccag ccgggacccg
    10981 gcaataaccg aagatgaaat cagggcgctg caattttctg ccggggatgt agcggaaata
    11041 gagcagacga ttctctcttt tgtcgatgcc tgccacaccc gtaaagtggc gatggtggtg
    11101 ggtagcacca ttaacaccct caaagatcgc gatggtaaac ggtggggaaa cctgccggat
    11161 atttattgcg cttatcttat ccgatgtctg gtttttcgcg gggaattagt gggatacggc
    11221 gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg ttaccttatg agtgcgcgca
    11281 tgtttgtctt gtgctgcatc tggtttattg tggcgttcct ctggataacc atcacttccg
    11341 cgctggacaa agagtggatg attgatggac gcgggattaa caacgtctgc gatgtgttga
    11401 tgtatctcga agaagacgat acccgtgatg ttggtgtgat aatgacgtta ccgctctttt
    11461 tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg ctggtttatg tacgccaccg
    11521 cgctggctat tttcggctac tggctgtggc agttttttct gcgctatcag ttttgtttgt
    11581 gagccggatt ggttcatccg gcacacaaac ttacaaactt cccgcctgac gacctttgcc
    11641 cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac gcatctggaa
    11701 gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca gctggtggat
    11761 ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca gctcgaaaat
    11821 ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt cgatcaccag
    11881 cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcgctat tggcatcggc
    11941 aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc agttgataaa
    12001 ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc agtcgctgat
    12061 ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg agatcgcgaa ttgtcaccgt
    12121 atcgccaatg cgaatcggtt tttcgaacag gataatcaga ccagagataa agttggcgaa
    12181 aatttcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc actgcaattt
    12241 cgaccactca ataccaatca ttgagaagcc gaccagcccg ccaatcagca tcagcagata
    12301 tttggtgatg gtggtgatgg cgtaacccgt acccggcgtt aaatccaggt gctgcaaaat
    12361 cgccagttcc agcagcgctg gcaggttgcg caccagctgc gtggtgatga taaataccag
    12421 aatggcaatt agcactgcac cgagggtaat cggctccagg ctttctacgc cctgtacagt
    12481 ggaagtgaca tcccacagag aaatattttc gaggaagccg aaagcggaat ggatttctga
    12541 ccacagcaca atgaccgaaa gcagggcgat caacatcaaa attgagcgca ccagccgcaa
    12601 ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa ttgccccttc
    12661 cgggctactg tgatgatgcg cttcctcttc gccacgcgcg cgttgcgcta acatctctgc
    12721 ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca tccagcggcg
    12781 gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt caagcctcgc
    12841 taacagcgcc tgtgccgttg ccagataacc cacagccgac gccagaatgg caaccaacgg
    12901 cgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc tgccctcttt
    12961 gttgagatac agtgggatcc cggccttttt caggctgagg gtgaccaccg ccagcgcacc
    13021 gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt cgtcgaggtt
    13081 atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca tcaggtagta
    13141 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
    13201 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
    13261 gccatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
    13321 cgtcatccac agcaccggca gcggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
    13381 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
    13441 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
    13501 cggcaaaatc gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
    13561 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagtggcc aggcgatggt
    13621 catcgggcgc acgtcagagg tccagaacag atagcgatgc gtcgcttcat tcacctcttt
    13681 cagcgcatcc tcaagttgcc cgttggagac tttcagcttg gtcagttcca gcagtagcgt
    13741 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct gtgcttccag
    13801 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatgaattt gccgtagcag
    13861 cggctgttta ttaagcagat cctcataacg taaccgttgc acacgcaact gcgccatttc
    13921 ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt gccacctgcg cccgcaacgc
    13981 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcagcgc
    14041 ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt tgcgaggcaa cgagatccat
    14101 ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg ttaattttga attgcgcgac
    14161 gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat tcggtacttt ccagcgcccg
    14221 ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca aatacgcatc
    14281 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctgacggtt
    14341 attggcagac agctgcgcca gttccagttc atcaacgagc gctttcaggc gtgcggagtc
    14401 agactgcaac gcgaaatttt gtgcctgatt gagcggggta ttgccagtaa gcgttcccag
    14461 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
    14521 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
    14581 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acatccccgg
    14641 cgacacgctg cgcggttcgt cacgcatgtt gtttaattgt gcgcgcagag tagcggaaag
    14701 tttcgggtaa ttatcaatga cttcttgata ttgcttgatg cgctcaaggg aaccttttcg
    14761 ttcctcaagc gcatttaagg cggactggag cgcctctacg acttccggct gcgcgggttt
    14821 cgccgctttt gcctgctcca gttcttgtga gatttgtttg ctatcggggg ccgtcgcggc
    14881 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
    14941 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
    15001 ttctgcttcg atctcttcag caggaagcgg ggccggttca gcgtctggcg taacaaaggt
    15061 ttcggtagat actgccagcg gctgaccaat tttcgtaaca gacaggcttt ccagttgctc
    15121 aaccaggttc actttacctg gtgcaaacag gttgataacg gtagagccga gtttaaagcg
    15181 ccccatttcc tggcctttca gcagtgccac agaaccgtct ttttccccgg caggccaggt
    15241 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
    15301 gctgcctaca atcgtcgccc cgaccagaat ctgcgccatc gggccaaatt cggtatcgaa
    15361 aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag ccgtgagatg
    15421 gttaacggag aagagatcgc ccggtacgta gatcatctca cgcagaatac cgttgcacgg
    15481 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
    15541 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
    15601 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
    15661 aggcatgacc aggacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
    15721 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
    15781 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
    15841 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
    15901 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
    15961 cggcctacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
    16021 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
    16081 acggtgatag ttttcgaaac gggtttccgc gatttttcct tcctcaaccg cttcccggat
    16141 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
    16201 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
    16261 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gtcgtgcggc
    16321 ggtcgtggtg tgctgaccga gacccgagtt gtcagagaca tcgttggtca ggatctcttt
    16381 ttgcagcccc agcaacgcgt tcagcaggct ggatttgccg acgccagact ggccggcaaa
    16441 aatgctgatg cgcccggtca acgcctcttc cagcggtttt agcccatcct gagtatggct
    16501 ggaaaccatc aatacgcgat aaccaatgtt gcggtagata tccatctgct cgttgacgaa
    16561 cgccatgcct tcgtcgtcca gcaggtctat cttattgagc acaataatcg gctcaatctg
    16621 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
    16681 ggcggagaca atgacaatct ggtcgatgtt ggcggcaata ggtttcacgc cgtcgtagaa
    16741 atccggacgc gtcaacactg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
    16801 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
    16861 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
    16921 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
    16981 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
    17041 ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt cgcccctgat
    17101 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
    17161 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
    17221 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaacctgaa
    17281 tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc tggcgctgat
    17341 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
    17401 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa tttttaaaac agtgggtgcc
    17461 tgcgggaaaa tcgccgattt gcggtaacag catcggtcag gaccgtcgtt tcctgtttaa
    17521 atacatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca gcaccctgaa
    17581 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
    17641 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
    17701 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
    17761 attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
    17821 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
    17881 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
    17941 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccag
    18001 tttaaaagac atcggcgtca agcggatgtc tggctgaaag gcctgaagaa tttggcgcgt
    18061 taacaaagcg gttatgtagc ggattgcaaa tccgtctagt ccggttcgac tccggaacgc
    18121 gcctccactt tcttcccgag cccggatggt ggaatcggta gacacaaggg atttaaaatc
    18181 cctcggcgtt cgcgctgtgc gggttcaagt cccgctccgg gtaccatggg aaagataaga
    18241 ataaaatcaa agcaataagc agtgtcgtga aaccaccttc gggtggtttt tttgtgcctg
    18301 caacttgtcg ttacaccctc cttaattttt aatcaccagc aaagccactc aaaaagtatc
    18361 tctgatacgg accggcatgt aagataggtg ctggcgagtt gagatccaca aggaaaagcg
    18421 tatgaaaacg ggaccgttaa acgaaagtga gttggaatgg ctggacgata ttctgaccaa
    18481 atacaacact gaccacgcca tccttgatgt ggcggagctg gacggtttat tgacggcggt
    18541 gttgagttct ccgcaagaga ttgaaccgga acagtggctg gttgccgtgt ggggtggggc
    18601 tgactatgtg ccgcgctggg cgtcagagaa agagatgacg cgctttatga atctggcttt
    18661 tcaacatatg gccgataccg cagagcgtct gaacgaattc ccggagcagt ttgagccgtt
    18721 attcggcttg cgagaagttg atggcagcga gctgacgatt gttgaggagt ggtgctttgg
    18781 ctatatgcgg ggcgtggcac tttctgactg gtcaacgttg cccgattcgt taaaaccagc
    18841 gctggaggcg attgcgctgc acggtactga ggaaaacttc gagcgggtag aaaagatgtc
    18901 gccagaagcg tttgaagaga gcgtagatgc cattcgactg gcggcgcttg atctacatgc
    18961 gtactggatg gcgcatccgc aggaaaaagc tgttcagcaa ccgatcaaag cagaagagaa
    19021 accggggcgt aacgatcctt gcccgtgcgg tagtggtaag aaatttaagc agtgctgcct
    19081 gcattaagag gccaaattgg gtgtcagcgt taattctggc acccaataag gaatttgagg
    19141 ctatctgatc accccacttc tggtaacaac cctgccgcaa tcaaaaattg cacgccaatc
    19201 acagcaatac cacagagaaa caccaccacc agcgccggac gaccaccttt gacccggtaa
    19261 cccgcctgag gattgtgctt tctgctttgc caggtcaaca gcgaagggat aatcaatgcc
    19321 agtaccgcca gcgccacacc ggcgtaaccc agcgccatca caaatcctcg tggataaaac
    19381 agtgcaaacg ccaacggcgg cagaaacgta attgcaccag tttgcaaccg tccaccaacg
    19441 gtatttgaac gctgaaataa atcagccaga taatcaaata agcctaacgc aacgccgaga
    19501 aatgacgtgg cgagggctaa atcagcaaat aaatgcactg ccagctcaac atgcggagag
    19561 gccaccattt cgcgtaacgc ctgtaacagc ccgtttaatc cagcatgatt agccagcaat
    19621 cccataaagg ttgttgaatc aatgctgcca agcgtcgcca cctgccagaa aatatatgcc
    19681 accaggggga tcgcactacc gattataaac acccagcgta gcttacgaat gttgccatcc
    19741 atatagctga caatactcgg cacgctaccg tgaaaaccaa acgacgtaaa aatcaccggg
    19801 attgcagaca gagccagccc ctgttgcaac ggcagggtta aaagattcac tttgtgaata
    19861 tgcggcagca gtagtaccag cattaccacc agaaaaataa tcttggcgct gaacagaaaa
    19921 cggttaaata aatcgaccag tgaagttccg acacaaacca cgccaccggc aacaaaagtg
    19981 aacaacagca cgccagcggt tgccgacata gaaatacctg tccagtcgct gatgctggag
    20041 gccaacaatt caccggcacc gctgatgtat gccgcagtca gagcatacat taagaacatc
    20101 atactgaagc ccgtcagcca ttgaccgtag cgtcccagat agcgttttgc cagcgtgccc
    20161 agaccggtat ctgccggaac atgctggtac acctccagca gtaatagcgc cgtgtagcac
    20221 atcaacgccc aaagcccaat caacaagatt aacgtaacgc taaaaccaac accggccgca
    20281 gccagcggca ttgccagcat gcctgcgcca attgtggttc ccgccacgat aaaaacactt
    20341 cccagggttc tgtttttcac gctttcttct gtcctgacga tctttatgag tgatatctgc
    20401 ggcgcaggtt aaggcataac ggctgcttcg tcaaaccgac gttacatatg gtgtaaatat
    20461 aaatgtacaa taaaaagcat tcagtcctgc gctggcgcaa agtcttcata agcggcaatc
    20521 gctacgctag gctttttttg aggagggacg atggactcta ttcacggtca tgaagtgtta
    20581 aatatgatga ttgaatcagg cgagcaatat acgcatgcca gtctggaagc tgcgattaaa
    20641 gcgcgttttg gtgaacaggc acgttttcac acctgctcgg cagaagggat gacagcggga
    20701 gagctggtag cgtttctggc agcaaaaggc aaatttatac cttcgaaaga cggtttttcg
    20761 accgatcaga gtaagatttg ccgtcactga tttgaaacgg cggcagtaaa cctgccgccg
    20821 gagagcatta gttttgtgtg tcgagggtag agagttcttt gtcgataaaa tacagacctt
    20881 cgccgctttt gcctgccagg cttaatttat caataatcga tttgaacagt ttctcttctt
    20941 catgctgctc agaaacatac cattgcagga aattaaatgt tgggtagtcc tgattggtca
    21001 ttgcagcatg agccagttcg ttaattttct gggtgattaa ttgttcgtgt ttataggttt
    21061 cctggaataa ttcatcaagt gaggaatatt cagcaaacgg agattcaacg gtattaatac
    21121 gcggtaaatt gccggtatca gtcaggtaat caaacagacg ctgcatatgc gtcatctctt
    21181 cctgggcgtg acggcgcagg aacgcggcag caccttcgaa ggtatgatag ctgcaccagg
    21241 cgctcatttg ctgataaagc agtgaagagt acagttccag gttcatctgc tcattaagtt
    21301 tttcaatcat ttctggtttc agcatgatag tgctccacaa aggttatatt tgttgctgtg
    21361 gcgtcactat aatttgtta
//
LOCUS       DAPPNJ010000068        21022 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000068 DAPPNJ010000000
VERSION     DAPPNJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.068"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            113..1810
                     /gene="ypdA"
                     /locus_tag="RPM73_002092"
     CDS             113..1810
                     /gene="ypdA"
                     /locus_tag="RPM73_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDX6520452.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1825..2559
                     /gene="ypdB"
                     /locus_tag="RPM73_002093"
     CDS             1825..2559
                     /gene="ypdB"
                     /locus_tag="RPM73_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDX6520453.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2572..3429
                     /locus_tag="RPM73_002094"
     CDS             2572..3429
                     /locus_tag="RPM73_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6520454.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3432..5927)
                     /gene="ptsP"
                     /locus_tag="RPM73_002095"
     CDS             complement(3432..5927)
                     /gene="ptsP"
                     /locus_tag="RPM73_002095"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX6520455.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYVGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGINHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5952..6989)
                     /gene="ypdE"
                     /locus_tag="RPM73_002096"
     CDS             complement(5952..6989)
                     /gene="ypdE"
                     /locus_tag="RPM73_002096"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX6520456.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRTVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6989..8074)
                     /gene="ypdF"
                     /locus_tag="RPM73_002097"
     CDS             complement(6989..8074)
                     /gene="ypdF"
                     /locus_tag="RPM73_002097"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX6520457.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8089..9336)
                     /locus_tag="RPM73_002098"
     CDS             complement(8089..9336)
                     /locus_tag="RPM73_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDX6520458.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9358..9684)
                     /locus_tag="RPM73_002099"
     CDS             complement(9358..9684)
                     /locus_tag="RPM73_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDX6520459.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9903..10868)
                     /gene="glk"
                     /locus_tag="RPM73_002100"
     CDS             complement(9903..10868)
                     /gene="glk"
                     /locus_tag="RPM73_002100"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDX6520460.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            11072..12328
                     /locus_tag="RPM73_002101"
     CDS             11072..12328
                     /locus_tag="RPM73_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDX6520461.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12443..12769
                     /locus_tag="RPM73_002102"
     CDS             12443..12769
                     /locus_tag="RPM73_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDX6520462.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12910..14148)
                     /locus_tag="RPM73_002103"
     CDS             complement(12910..14148)
                     /locus_tag="RPM73_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDX6520463.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGVSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14484..15686
                     /gene="nupC"
                     /locus_tag="RPM73_002104"
     CDS             14484..15686
                     /gene="nupC"
                     /locus_tag="RPM73_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDX6520464.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15736..17925)
                     /locus_tag="RPM73_002105"
     CDS             complement(15736..17925)
                     /locus_tag="RPM73_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDX6520465.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18134..18209)
                     /locus_tag="RPM73_002106"
     tRNA            complement(18134..18209)
                     /locus_tag="RPM73_002106"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18174..18176),aa:Ala,seq:ggc)
     gene            complement(18249..18324)
                     /locus_tag="RPM73_002107"
     tRNA            complement(18249..18324)
                     /locus_tag="RPM73_002107"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18289..18291),aa:Ala,seq:ggc)
     gene            18545..18904
                     /locus_tag="RPM73_002108"
     CDS             18545..18904
                     /locus_tag="RPM73_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDX6520466.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18906..19298
                     /locus_tag="RPM73_002109"
     CDS             18906..19298
                     /locus_tag="RPM73_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDX6520467.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19350..20765)
                     /gene="gltX"
                     /locus_tag="RPM73_002110"
     CDS             complement(19350..20765)
                     /gene="gltX"
                     /locus_tag="RPM73_002110"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDX6520468.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         5188 a         5614 c         5222 g         4998 t
ORIGIN      
        1 atttatcttt caatcccctt ttaaatttat ggtttttcct caaaaacggc ctgctggtca
       61 tcagccttca ggttacctat catagaggtt taatccttat tcagagtcac ccgtgcacga
      121 aatattcaac atgctgctgg cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt
      181 ctttctcatc cgtatccgcc tgtttcgcga actgttgcac aagtcggcgc actccccaaa
      241 agaattgctc gccgttaccg ccattttctc gctgttcgcc ctgttcagca cctggtccgg
      301 cgttcccgta gaaggctcgc tggtgaacgt acgtattatc gcggtgatgt ccggcgggat
      361 tctgtttggc ccgtgggtag gcatcattac cggcgtgatt gcgggtattc accggtattt
      421 aattgatatc ggcggcgtga cggcgatccc ctgctttatc accagcattc tggcgggttg
      481 tatatcaggc tggataaacc tgaaaatccc caaagcacag cgctggcgcg tcggtattct
      541 cggcggcatg ttgtgtgaga cgttgaccat gattctggta attgtctggg caccaactac
      601 cgcgttgggg atcgatatcg tctctaaaat cggcattcca atgatcctcg gtagcgtctg
      661 tatcggcttt attgtgcttc tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc
      721 gcggcaggcc aagctggcgc tggatatcgc caacaaaacg ctacctctgt ttcgccatgt
      781 caatagcgag tcattacgca aagtttgtga aattattcgc gatgacattc acgccgatgc
      841 ggtggcgatt accaataccg atcatgtgct ggcctatgtt ggcgtgggtg aacataacta
      901 tcagaatggc gatgacttca ttagcccgac tacccgtcag gcgatgaatt acggaaaaat
      961 catcattaaa aacaatgatg aagcccaccg cacaccagag attcattcca tgctggtgat
     1021 cccattgtgg gagaaaggcg ttgtgaccgg aacgctgaaa atttactact gccacgcgca
     1081 tcagatcacc tcgtcattac aggaaatggc ggtcggtctg tcgcaaatca tctccaccca
     1141 actggaggtt tcacgcgccg agcagctacg tgaaatggca aataaggcag agcttcgcgc
     1201 cctgcaaagc aaaattaatc cccatttcct gtttaacgct ttgaacgcta tttcatcatc
     1261 aatccgtctg aatccggata ctgctcgcca gctgatcttt aatctgtcgc gttatctgcg
     1321 ctataacatt gaattaaaag acgatgagca aatcgatatc aaaaaagagc tgtatcaaat
     1381 taaagactat attgccattg agcaggcccg ctttggcgac aagctgacgg ttatctatga
     1441 tattgatgaa gaggtgaatt gctgcattcc cagcctgctg atccagccgt tggtggagaa
     1501 tgccattgtc cacggtattc agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc
     1561 agagtgcggc aatcgggtac gcattgcggt gcgggatacc gggcacggca tcgatccaaa
     1621 ggtgattgag cgggtcgaag cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt
     1681 tcatcatcgc gtgaagttat tgtatggcga ggggctgcat atccgccgcc tggaaccagg
     1741 aacggaaatt gcgttttaca ttcctaacca acgcacccca gtcgcctcac aggctacgtt
     1801 attgctttga gccggagtga tattgtgaaa gtcatcattg ttgaagacga attcctggca
     1861 caacaggaac tgagctggct aattaaagag cacagccaga tggagattgt cggcaccttt
     1921 gacgacggtc tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc catttttctg
     1981 gatatcaata ttccgtcgct ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc
     2041 cataaaccgt ttattgtgtt catcaccgcg tggaaagaac atgcggtaga agcgtttgaa
     2101 ctggaggcgt ttgactacat tctcaaaccg tatcaggagt cacgtattac cgggatgctg
     2161 caaaaactgg aagcggcctg gcaacaacag cagaccagca gtacgcctgc cgcgacggta
     2221 acgcgtgaga atgacaccat taatctggtg aaagatgagc gaataatcgt cacgccaatt
     2281 aacgatatct attacgccga agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa
     2341 tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca aactgccgcc gtcgcatttt
     2401 ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg
     2461 tttaataaca cctacattct gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc
     2521 agcaaagtga aagaatttcg ccagttaatg catctttaat cgggaacttt catgaaagca
     2581 ccaggcttgc ccgccgatca acaatttttc gccgatctgt tcagcggcct ggtgcttaac
     2641 ccgcaactac tggggcgcgt ctggtttgcc agccagcctg cctcgttacc ggtgggcagt
     2701 ttatgtattg attttccccg tctggatatc gtgctgcgcg gcgaatacgg caacctgctg
     2761 gaagcaaagc agcagcgaat ggtggaagga gaaatgctgt ttattccggc acgcgcggct
     2821 aatttaccga tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc accgacctgg
     2881 cttgggttat cgttttacga tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc
     2941 cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc ttaccgccct cacccatctt
     3001 agccgttcgc cgctggagca gaatatcatt cagccactgg tgttaagttt gctgcatctt
     3061 tgccgtaacg tggtgaatat gccgccgggc aattcgcagc cgcgcggcga ttttctctat
     3121 cacagcattt gtaactgggt tcaggataat tatgcccagc cgctcacccg cgagagcgtg
     3181 gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg
     3241 atgcgtttta tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat gattttgcag
     3301 aaatatcatc tgtcaattca tgaagtggca cagcgttgcg gttttccgga tagcgactat
     3361 ttttgtcgcg ttttccggcg tcagtttggt ctgacgccgg gagagtacag cgcccgtttt
     3421 cagggctaac gtcagaaggt taattccgtt tccagcagcg tcaggatgct ttgcgcatct
     3481 tgcgcggcaa acaacgactg gcggaagttt ttattcacca gtttacgcgc cagctgcgag
     3541 aagactttca catgattaat cccttcgtta gcacccagcg tcagcatgat caccagttcg
     3601 acttcgccca tttctgactg ccagtcaacc ggtttcgcca gccgggcaat gctgatactg
     3661 gaatgacgga tccactgaga tttggtgtgc gggatcgcca cgccaaaacc aacgccagtg
     3721 gtgacaatct cttcccgctg ccagacatcc tcttccagct cgaacggatg ttcagtgcgc
     3781 ccgttaacgc cgaggttacc gcacaggaac tggatcgcct gctctttatt gctaaaggcc
     3841 tgatcaacga agatattctc cagcgccaac aatgggcgaa cgtcttcttc cggcgtaaag
     3901 gcggtgagta acgcttcaat ttcctgcgcg ctgcgacatt cacacgcctg acgtgccagt
     3961 tcccgacacg cctcgctatc cagttgacgc agctggcttt tcaccgccgg aatgcgcggg
     4021 ctactcatac tcagctcatc caggcccagc ccaagcagta gcggcagata acgactttca
     4081 ccgcccagtt caccgcaaat gcctacccat ttgccccgct gatgcgcagt ggtaactatt
     4141 tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat acagcggcga tacgcgcggg
     4201 ttattacgat cgaccgcata cagatactgg gtcatatcgt tagaaccgat actgaagaaa
     4261 tcgacctcat cgcagaagtg gtcgatgatg taacacaccg acggcacttc caccatgata
     4321 cccagagtaa tcgtctctgc atgacgcagg ccatcgcgct taagctcaac gatcgctttt
     4381 tggatctcgc ccttcaccca taagatctga tcgaggctgt gaaccatcgg gatcatcaac
     4441 tgggcgttgc caaaactggc agcgcgcaaa atcgcccgta gttgagtgcg gaacaggcca
     4501 gcaaattccg ggtaaatacg taccgcgcga tagccgagga acgggttctc ttcctgggga
     4561 atattcagat aaggaatgct tttatcgcct ccgatgtcca ttgtgcggaa gataatcggc
     4621 ttgtcgcccg ctgccagtag cacctgctgg taggcttcaa attgctcctg ctcgtccggc
     4681 gcgctgtcac gatccatata cagcatttcg gtacggaaca gcccgacgcc ttccgcgccg
     4741 ttggcaaacg cgcctggtgc ttccagagcg gtgccaatat tcgccgcgat atcaatacgc
     4801 ttgttatcac gggaataggc cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta
     4861 tccgccagcg tctgcgcaac ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc
     4921 agcacgccgc actgggcgtc aagcaccgcc ggttgcccga cataacgggc aatcgcatcc
     4981 agcggcaggc cgctcagtac cgggatcgcc gaggcgcggg ccagaatcag ggtatgcgag
     5041 gtgcgcccgg ttttctccag aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac
     5101 tggcttgggg ttaaatcttc agccaccaga atggtcggtt tttccagcac cagattgttg
     5161 cgcggcttca gttccggcca ggtgatatgc agcaactgtt cgctaatatc gcgaatgtcg
     5221 ctaacacgtt cgcgcagata atcgctggca gaagcagaaa gcttggcgca aacctgctcc
     5281 atattgctga tgatcgccgc ccccagtccc tgatgctgtt ctgtcatcag gcgacggata
     5341 ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg cgctgaggat agttttgctt
     5401 tcgccgtcac gctcacgcaa ttgctggttc agttgctcgg caagcgttgc caggctgtgc
     5461 tccagccggg tggaatcttg cgcactggcg gggattgccc gataactgtc gaggctgtcg
     5521 ctttgtaaca gggtcagcgt acccacgccg acgccgcttg ccagcacatt gccgtacagt
     5581 aaatccgggt taaggcggct taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc
     5641 gtcggctgaa cgctgtcgct gtcgataaag cgcaccagga tgtactcttc cagcacgcgc
     5701 cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc aactgtcatt aaataaggtg
     5761 ccagttccaa tcaacgccag cgagctttta gcatcggcct ttgcgttctg gcgatggttg
     5821 aaaaaggtga tttcgctttg ccactggctg cactgttctt taagttccca cgccggacga
     5881 gcatgtagac cgttaggcaa aggacagaga aattgaatcg ttaacataac cgctccgggt
     5941 taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgcgt aagacgttga
     6001 ataagggcag ataaaagttg ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc
     6061 gcgcaatgtc cgtggcgggt ggcaggcccc attaccactg tgggtacgcc agtaccggtt
     6121 aagtgcaccg ctccgccgtc cgtgccgccg ttgctgaaca tatctgcctg caacggtacg
     6181 ccaatttcag ccgccacggt ttcgatccag gcggtaagtt ttggcggcgc aatcagtgac
     6241 ttgtcgctta acaccagcat cgggccgtta ccaatctggc gatggttagc cgcgccataa
     6301 tcaaagtttt tcgcccagca ggcggtatcc agcacaatgg cgacgtccgg cgacaccgtg
     6361 cgggtagcgg tttgcccacc gcgtaatccc acctcttcgc tggaacttgc caccagccac
     6421 acctccgcag gcagttcggc atcgtgcagc tcacgtagta acgtcaccag cagatagcaa
     6481 ccgaggcgat catcaaaggc tttccccatc acccgctggt gagggagaac ctgaaaagtt
     6541 gtatcaaagg tgacgcgatc gcctggacga attcccgcct gcatcacttc gtcatacgag
     6601 cgcgcaccaa tgtccacgcg catggcgctg acgtcattcc cctgccggtc gccgtcaagc
     6661 aggcctggaa ttttgcactc ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca
     6721 gccatgcgca cgttgccaac cggcagcaca tcaatcgccc cttcgcggga gatgctacgc
     6781 accataaatc ccacttcgtc catatgggcg cagatcatca ccttcggacc tgtcgattca
     6841 ttgaggcgga tcagcaccga tcccagacca tcaaatcgca cttctttttg caggcgatcc
     6901 gcttcttcca gcaggatctg ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg
     6961 ctcaacgctt ttaatagcga taaatccatt atgcctctcc cgtgagcaac actgttttcg
     7021 gcatggcgta gagcacttct gcgccttgcg gggtaaccag cacaacatct tcgatgcgca
     7081 cgcccccttg ccctggcaaa taaatccccg gctccacggt cagtaacatg cctggctgta
     7141 gcgtcgtggt gtcccgcggt gaaaaacgcg gatcttcatg gacttcaatg ccgatagcgt
     7201 gaccagtgtt atgaccgaaa tagtcgccaa atccagcctc ggtaatgacc cggcgcgcgg
     7261 cgtcgtcaat ctgctggcag cgcacgccgg ggcgaattgc agagattgcc gcgagctgtg
     7321 cctgcaggac aatctgatag acgtcaaaca gcgggtgaga ttcggcgctc accccttcgc
     7381 cattcaccag caaggtgcgc gtcatatcag agcagtagcc ctgatacagc gcaccaaaat
     7441 cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg
     7501 ccccgcgcca gccgctggcg acaatggtgt caaaagaggc tttttctgcg ccctgctggc
     7561 gcataaacca ctccagttca gcggctatct cgcgctcgct catccccgcc tgaataaagc
     7621 ggcgaatatg ctccgctccg cgatcggcaa tcccacaggc gaggcggatt atctccacct
     7681 cctctggcgt tttgatttgc cgcagcacat ccggcgtggc gctaaccagt ttcgcattga
     7741 gttcagactt ccagcggtgc gcggtttccc aactcacctg ctggccttca aaaccgagcg
     7801 tttgcaactg ctcatcggca atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt
     7861 caagcaaatg cagctggtag ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat
     7921 ctaccagaat gtgcgcactt tcacggctaa taaccacata gcctgatccg gtggagatcc
     7981 ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac tgcatccagt tgttgcgcct
     8041 taagccagtc gcgcagcgaa gcgagtaatg tcatctggat tttccttctt acaggctatc
     8101 gattaacaat ttgcctttac ggaacatcat cagacgcagg aacaccacca tcagcgcagt
     8161 tatgactgcc cccagtgcga tccccgccat atagacgccc aggttagtga ccagcggcca
     8221 tgcccagata gcggattccg ggaaccattg cactgcgccc agccagacag cggcggttga
     8281 gccgacaatc gcgccgacca tatacgacgg aatggcggtg atggggcttt ccagcgcaaa
     8341 cggaatcgca ccctcactaa tccccataaa ggcaaggaac atcgcggtct taccctgcgg
     8401 ataaagttga gcgttgaaca ggcgtttgcc ggttaaacga cggtcaataa tggtcgccag
     8461 acccagacca atcggaggaa taacgatagc gatagaacga gcggtgaccg gtaatacgtg
     8521 gtcggtggta aagctgaagg caacgaaacc tgccgctttg ttgattgggc caccaaggtc
     8581 gattgcggtc gccgcggcaa tccccattgc atacatcagc gcgcccttct cacctgcggc
     8641 agtcagcacg gtacggatac cgccgttgat ccagccacca aatggggtga tgacatagta
     8701 catcgccagc atgacaaaaa tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc
     8761 taacaggaaa tctggtaact ggattttctg gttcatccat ttcacaaggt aaccagcaac
     8821 gatagatata atcagcgcac caatgaaggt ggacggcacc ggcgaagagg tcgcccattg
     8881 catagtgctg ggatcgaagt tcagcagttg cgtcggctgg gtagacatca agccgccgat
     8941 aaaaccagcc gggaacgcca gtttgccgcc gatagagttc gccacaaaag cggcaaacat
     9001 cggaatagca aagccaaaca acacgccgcc gaaggactgc gacagccagg caaatttcag
     9061 taaggagagg tcgaaaccgg agaatttccc gctattaaga gcatccatga tgccgatctc
     9121 agcaggaatt ttaagccagc tataagcaat cagctggctg aaagcgagga tcacaccgcc
     9181 cataattaag gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc
     9241 ccagaagctg cttttagagg cctgcggatt cttaactgcc gctgccgcac cggatgcgcc
     9301 aggcacaacg gttgcactgc gttttttaat ggccataaca attatccctg tcgaatatta
     9361 ctgttgttca gaggcaatca tctcttcgat ttctttgatg atgcccgcag cgtttttaat
     9421 tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg
     9481 ggtaactgcc acggagtgga tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg
     9541 attctggata ccgtccgcgc cctgggtttc aattttcact tcataaccgg cttctaccgc
     9601 cgcttcttcc aacgcctgag cggccataaa ggtgtgagcc aggcccattg ggcaggcaca
     9661 taaggcaatc agtttcttac tcatcgttaa ttcctcattc agtgatagtt gacgagtgaa
     9721 attatgaacg tgcaaaacaa atcgccgtta ccagacaaat aatgcattta ctggagaatt
     9781 aatcccgctg tacggaagtg tgacgcaggt cggccttgtg aggggacaca aagaaaaatt
     9841 gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat aacaggaagg
     9901 atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac cggaaccgag aagacccgga
     9961 ttgttatgga cgatgagata caccggaata tcatggacat attctttaaa gcgtccttta
    10021 tcttcaaatg cggcacggaa accagaggct ttgaagaact caaggaagcg cggcacgata
    10081 ccgccagcaa tataaacccc accaaaggtt cccagcgtta atgccagatt gccgccaaaa
    10141 cggcccataa tgacgcaaaa cagcgacaac gcacggcggc aatcggtgca gctgtcagcc
    10201 agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt gtcggcttta
    10261 acaattgcgc gatacaaatt caccagccca ggaccagaaa gcacgcgttc cgccgaaaca
    10321 tgaccaattt ccgcacgcaa tatttcgagg ataatggcct cttcttcact attcggcgca
    10381 aaatcaacgt gaccgccttc gcccggcaag cttacccaac gcttatcgac atggaccaga
    10441 tgcgcaaccc caagccctgt tccggcaccg taaaccgcaa taggtttacc ttcgaccggt
    10501 tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc catcgataca
    10561 gcggtaaaat cgttaataat ttccagatgg ctaaaaccga gattcttttt catttcggca
    10621 attgagaacg cccaggtatg gttggtcatt gccacccagt caccggtaat tgggcaagcg
    10681 atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac gcgaatgacc
    10741 gcttcgaggc tggggtaatc aagccctgaa taggtcttag cctgcgagat ttcaccactg
    10801 gcaatatcac acagagcaag acgtgcgttg gtgccgccca catcaccgac taatgcatac
    10861 tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca ctgtaaatac
    10921 ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga tctgggtcac
    10981 acatttactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac
    11041 taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca gaaccatgtt
    11101 gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc tgattgtggt
    11161 gatgaaaatc gcctcggtat tacagaattt gctctggcaa cgactgccgg gaactctggg
    11221 gatagcccag gattcacctc tctggatcat cggtgtatta acgctaacgg gtattgcggt
    11281 ggggttggtt atccgtttca gccagggtca tgccggacca gaccccgcct gtgaaccgct
    11341 gatcggcgca ccagttccgc cctctgcgct accaggactt atcgtagcat taattctcgg
    11401 tcttgctggc ggcgtcagcc tggggccgga acatccgatc atgaccgtca atatcgccct
    11461 tgcggttgcc attggcgctc gtctgttacc gcgcgtcaac cgaatggagt ggactatttt
    11521 agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt
    11581 ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtcttt ttgcgccatt
    11641 aatggcggca gcagctggtg cacttactac cggattattt ttccatcccc atttttcact
    11701 gcccattgcg cattacggac agatggaaat gaccgatatt ctcagcggtg caattgtcgc
    11761 ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc ttaccacggt tgcacgcgat
    11821 gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta ttctcggtat
    11881 tctgggggtt attggtggac cagtttcgct gtttaaaggg ctggatgaga tgcagcagat
    11941 ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtaa ttaaacttgc
    12001 cgccctggtc gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt
    12061 tgtcggcgtg gcattagggt tgatgctgca tgagcacgta cccgccgtac cagcggcaat
    12121 aaccgtttct tgcgctattc tcggcatcgt gctggtggta acacgcgatg gctggttaag
    12181 tctttttatg gcggcggtcg ttgtacccaa taccacattg ctaccgctgc tctgtatcgt
    12241 catgcttccg gcatggctgt tattagcagg taagccgatg atgatggtca atcgtccgaa
    12301 gcaacagcca ccccacgata acgtttagca aaaaatgctc tcctttatga tttaagagtt
    12361 atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc gctgacggct
    12421 tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg ccctgatggc
    12481 atttaccccg cttgcagcaa acgcaggtga aatcacccta ctgccatcaa tcaaattaca
    12541 aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc gcgaccgtga
    12601 ctactggcat cgcaattatg agtggcgtaa aaaccgctgg tggcgtcatg ataatggcta
    12661 tcatcgtggc tgggataagc gtaaagcgta tgagcgtggc tatcgtgaag gctggcgcga
    12721 tcgtgacgat catcgcggaa aaggccgcgg acatggacac cgccattaaa cacattcgcc
    12781 aatggaacac aatgcctgat gcgacgctag cgagtcttat caggcctaca aaccggctct
    12841 ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg
    12901 ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca gcgccacgac
    12961 cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat
    13021 caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga taccaaaact
    13081 taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca gaatgacaat
    13141 aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga agcgaatgaa
    13201 tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca gtccggcagc
    13261 aaccagactt accccaaaga ccgttgccgc agcatggctt aacagcggtt gcagcgtcag
    13321 ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg ccgccgcagc
    13381 tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag ccacatccca
    13441 tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag tgagcgaaga
    13501 gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg ccaggaagac
    13561 cgcttccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg ccaggttagg
    13621 ctgggaaaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta acccaccaat
    13681 cactttttcc agcggttttt gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat
    13741 ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa aaccgatcgc
    13801 cgcgccaata aattccgcca ggtcggttgc catcgcaata atttctgcct gaacccaata
    13861 gaaccacact acgggacgcg gatagtgatc gcgaatctgc tccgccagat ttttaccggt
    13921 ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt tggcccaaac
    13981 gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg tcgcaaagtt
    14041 accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg cgagcctcat
    14101 cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct tgtgcctcta
    14161 aaacatagcc tttgctatgt ttcatgctat gccaaacgag aatgattatc aaattcattt
    14221 aaatgggttg tggtgatttc tctgatagac ctggattatg actacgaaaa gattgatgac
    14281 ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc ttccatacac
    14341 aacatagcag aaatgtatga cagatcacta tttttgaagc ttgtcacagg acgtcattat
    14401 agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac ggaaatttaa
    14461 cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg gcacttgccg
    14521 ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg
    14581 ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac tccgacgttg
    14641 gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg
    14701 aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc ttcttcctga
    14761 aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag cacattcgcg
    14821 tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac ggcatgggca
    14881 aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa aactttattg
    14941 cctataaaga tatcctcggc aaaatctccc gcaatcgtat gtacaccatg gcagcaacgg
    15001 cgatgtccac cgtatctatg tccatcgttg gtgcatacat gaccatgctg gagccgaaat
    15061 atgtcgttgc ggcgctggta ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca
    15121 acccttaccg tgttgatgcc agtgaagaaa acattcagat gtccaacctg cacgaaggtc
    15181 agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaggtt gccattatcg
    15241 ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc actgaacgca ctgtttgcta
    15301 ctgtgactgg ctggtttggc tacagcatct ccttccaggg catcctgggc tacatcttct
    15361 atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca
    15421 tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag aaaattgctt
    15481 ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc ttcgctaact
    15541 tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg
    15601 tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt gtgctgtctg
    15661 cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga tcattccccg
    15721 gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat acccttgcag
    15781 atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga caaactccgc
    15841 gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa tcatcgcatc
    15901 cagcgtgttc gtgacaatat ctttcacaaa gacgccatca attttgatga tatcagcctg
    15961 caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa tcgcaatccg
    16021 gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc
    16081 ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac gcttaaacag
    16141 acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta aattgactga
    16201 aaagcgcggt ccttttttgt cgcaagggtg tgttgccaac cacttcaaca aggattccag
    16261 cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca gaaacttatc
    16321 cggggtcata atgccgccgt catacttcag tcgcgcgagg atctcatcat aaccttcacc
    16381 ctctttgttg cgaattggct gggcgtagag cagtaaatca ccctgatcta acgccgtgcg
    16441 aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct cctctctgct
    16501 atccagcgcc agcacatggt gatgtgcgca ggattgctcc gccagccagc ttaactgccc
    16561 caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag caccatagcc
    16621 catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt taaccatatg
    16681 ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac tacccggcaa
    16741 ttgatataac ttttcgtttt cctgcatcaa cggcagcaga gtacggcaaa ttgagcggat
    16801 acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat tatcaatgcg
    16861 caggcagcaa aaactcttgc ccgcctcttg ctccggcgct tgttccaacg cacggaagtt
    16921 gggcaggagc gttaacggat cggtcagcgc ctgcaaatgc cagcggcgat taagccattc
    16981 actgcgatga taaatgcgca ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc
    17041 gagaataaat gccagcgaat attcggtttc caccccttgc aaaaagttct ggttgtaatt
    17101 cagcaggcaa agcgttgaaa cagcccaggt gagatttaaa aacggatagc gaagcttacc
    17161 gaccccgagg gtaaagatga tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt
    17221 ttcataaggt gtgcacaaca gaagtagcag cacgcttaac gctgccagcc agcttaaggt
    17281 aaatgcgcgt ttctctttgc ccaacgacgg agcgatatcc ctgcgccaca atatctgcgc
    17341 aaagtgggga cttacaatca tgcgagtgag atagtagaaa agcatgttgt aaatcagcac
    17401 agcggtgaaa aggcttagta aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa
    17461 ggtggatatc tttagcggga aatcaaagaa gctccccaca agatacatgc tgcatttgat
    17521 gccgatcggc gtcaccaaac caagccagaa cagacgttgc cagacatatc ggctggtcaa
    17581 tccataacgc caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac acaacacgac
    17641 aaacgtctgg ctaccgagca tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag
    17701 attggtgcaa aacatcccgc ataaaacggg aacgacaccg cgccagccaa agataaacaa
    17761 gattgacagc attacgcaca gtggcatcca cgccagaaag atataactgg aatggataat
    17821 ggcaagtggc gaaataaaac gggatagctg aataagtacc acggtgagcg taaacgctag
    17881 tgtgaatatc ttgatatttt ttatcaggtt atgctccaca aacatgagta cactactctc
    17941 actgatcggg gttttcaggc acacaaataa taagtttatt cgcatcgcga agcaaacagg
    18001 gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc actaacccgg
    18061 acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg
    18121 agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc
    18181 aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa tttgctgggt
    18241 gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
    18301 ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca
    18361 tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa
    18421 aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg
    18481 tttcctgtaa agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat
    18541 tgttatgttc aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg
    18601 ccagaccatt aataaatggg tacgcaagga aggctggacg acgtcaccaa aaccaggcgt
    18661 ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgtaa
    18721 tgctgaacgt ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga
    18781 agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc
    18841 cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa
    18901 ataatatgaa aagattacgc aataaaatga ccaccgaaga actggctgaa tgcctcggtg
    18961 tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc
    19021 ccggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta
    19081 ttcagaacac gcctgcgttc cataacacac cgatgctcct ggaagccgaa gaaccccttg
    19141 ccgaatacgc accggggatt cgcactcctg cttatcggca aatcatcagc gcaatagaca
    19201 acatgactca cagtgagcag gaaaaagttg cgcaattttt gtcacgcgaa ggaattcgta
    19261 attttcttgc tcgtctcgac atagacgaat cagcataaat aaaaaacggc aggatattat
    19321 ctcctgccgt ttatcttttt acacgctaat tactgctgat tttcgcgttc agcaataaaa
    19381 tccagcgctt tgttgatacg ctcgatactg cgggtcttac caattgcgtg aacggtaaca
    19441 tccagtgctg gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta
    19501 cccataccca cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg
    19561 gtccagtcag taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc
    19621 ggacgcagat gttttttcgc cgcatcggca tcgaactcag caaaatcttc gtagaaataa
    19681 cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga
    19741 tcagccagct gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccactgtaag
    19801 tgagtagcaa catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc
    19861 ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc
    19921 atctcttcac gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag
    19981 ttcagcagtg cttctggcaa ataaccgtca tcacgatact gcattacgct gactgccccg
    20041 tgacgtttgg acagtttttt accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc
    20101 ggcaccggcg ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg
    20161 tcttcgccac ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag
    20221 ttataggttg gggaaccatc ggtacggcgg ataatcagat catccagctc ctggttgctg
    20281 aactcgatcg gaccacggat ctgatcgtca aaaacaacag aaccttcctg cggattagca
    20341 aaacgcacga cacacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg
    20401 tcataacgcg gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct
    20461 ttagagcaat agcatttata agcagtgccc tcttccagca tctgatcgat caccgcattg
    20521 tagcgatcaa aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag
    20581 ttcatgccat ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct
    20641 tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca
    20701 gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt
    20761 ttcatgaaat ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg
    20821 agtgggcgat attctatcac tcccgcctga ttcctcaatg tagttcgggt ttctacctta
    20881 cgcattattg ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt
    20941 aaatttacga cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc
    21001 tataatgcga ctccacacag cg
//
LOCUS       DAPPNJ010000069        21018 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000069 DAPPNJ010000000
VERSION     DAPPNJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.069"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            49..1026
                     /gene="pspF"
                     /locus_tag="RPM73_002111"
     CDS             49..1026
                     /gene="pspF"
                     /locus_tag="RPM73_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDX6520469.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDTIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(1146..2411)
                     /gene="puuE"
                     /locus_tag="RPM73_002112"
     CDS             complement(1146..2411)
                     /gene="puuE"
                     /locus_tag="RPM73_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDX6520470.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(2449..3729)
                     /gene="puuB"
                     /locus_tag="RPM73_002113"
     CDS             complement(2449..3729)
                     /gene="puuB"
                     /locus_tag="RPM73_002113"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDX6520471.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(3731..5218)
                     /gene="puuC"
                     /locus_tag="RPM73_002114"
     CDS             complement(3731..5218)
                     /gene="puuC"
                     /locus_tag="RPM73_002114"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDX6520472.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(5493..6050)
                     /gene="puuR"
                     /locus_tag="RPM73_002115"
     CDS             complement(5493..6050)
                     /gene="puuR"
                     /locus_tag="RPM73_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDX6520473.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(6077..6841)
                     /gene="puuD"
                     /locus_tag="RPM73_002116"
     CDS             complement(6077..6841)
                     /gene="puuD"
                     /locus_tag="RPM73_002116"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDX6520474.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            7053..8471
                     /gene="puuA"
                     /locus_tag="RPM73_002117"
     CDS             7053..8471
                     /gene="puuA"
                     /locus_tag="RPM73_002117"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDX6520475.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            8620..8784
                     /locus_tag="RPM73_002118"
     CDS             8620..8784
                     /locus_tag="RPM73_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520476.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            8774..10159
                     /gene="puuP"
                     /locus_tag="RPM73_002119"
     CDS             8774..10159
                     /gene="puuP"
                     /locus_tag="RPM73_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDX6520477.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            10293..10538
                     /locus_tag="RPM73_002120"
     CDS             10293..10538
                     /locus_tag="RPM73_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDX6520478.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            10851..12494
                     /gene="sapA"
                     /locus_tag="RPM73_002121"
     CDS             10851..12494
                     /gene="sapA"
                     /locus_tag="RPM73_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDX6520479.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            12491..13456
                     /gene="sapB"
                     /locus_tag="RPM73_002122"
     CDS             12491..13456
                     /gene="sapB"
                     /locus_tag="RPM73_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDX6520480.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            13443..14333
                     /gene="sapC"
                     /locus_tag="RPM73_002123"
     CDS             13443..14333
                     /gene="sapC"
                     /locus_tag="RPM73_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDX6520481.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            14333..15325
                     /gene="sapD"
                     /locus_tag="RPM73_002124"
     CDS             14333..15325
                     /gene="sapD"
                     /locus_tag="RPM73_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDX6520482.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            15327..16133
                     /gene="sapF"
                     /locus_tag="RPM73_002125"
     CDS             15327..16133
                     /gene="sapF"
                     /locus_tag="RPM73_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDX6520483.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            16201..16554
                     /locus_tag="RPM73_002126"
     CDS             16201..16554
                     /locus_tag="RPM73_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDX6520484.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            16922..17710
                     /gene="fabI"
                     /locus_tag="RPM73_002127"
     CDS             16922..17710
                     /gene="fabI"
                     /locus_tag="RPM73_002127"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDX6520485.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            17855..18982
                     /locus_tag="RPM73_002128"
     CDS             17855..18982
                     /locus_tag="RPM73_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDX6520486.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            19050..20984
                     /gene="rnb"
                     /locus_tag="RPM73_002129"
     CDS             19050..20984
                     /gene="rnb"
                     /locus_tag="RPM73_002129"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDX6520487.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         4685 a         5592 c         5597 g         5144 t
ORIGIN      
        1 atgccaggat gagttagcga attacactaa caagtggcga atttcatcat ggcagaatac
       61 aaagataatt tacttggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat
      121 ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg taaagagctg
      181 attgccagcc gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac
      241 tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg
      301 tttaccggtg cgcaaaaacg tcatcctggg agatttgaac gtgccgacgg cggtacgcta
      361 tttcttgatg aactcgctac ggcaccgatg atggtgcagg agaaattatt gcgcgtgatt
      421 gagtacggtg aactggagcg cgttggcggc agccagccat tgcaggtgaa tgtgcggttg
      481 gtatgcgcga cgaatgccga tctcccggcg atggtcaatg aaggcacttt tcgcgctgac
      541 ctgctcgacc gactggcttt tgatgttgta caactgccac cactgcgcga gcgcgaaagc
      601 gacataatgt tgatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct
      661 ctgttcccgg ggtttacgga gcgcgccaga gaaacattgc tgaattatcg ttggccggga
      721 aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat
      781 tatccgcttg atgacatcat tattgatccc tttaaacggc gtccgcctga agacactatc
      841 gccgtttcag aaaccacctc gcttccaaca ctgccgctgg atttacgtga gtttcagatg
      901 cagcaggaaa aagagttgct gcaactcagt ttgcaacaag ggaaatataa ccagaagcgc
      961 gcggctgaat tactggggtt aacttatcat cagttccgcg cgttgttgaa aaagcaccag
     1021 atttagcgaa gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt attcggcata
     1081 cataatcaat acgtcgtttg caggcctgat aagcgcagcg catcagacat tattgcgttg
     1141 ggctattaat cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcgcatcc
     1201 gggatggtca gcggatacag gaagcgaatc acgttgccgt atgcgccaca ggtcagcagg
     1261 agcagcccct gcgccagcgc gcgttgctgg attttctgtg caatcgccgc tgacggctcg
     1321 cccgtttgcg gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg taccgcagca
     1381 atggccggaa cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg gcccagttga
     1441 ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga gcaccgcgtg cgcggcagcc
     1501 accgccagcg ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg cgcgtccata
     1561 atattcgcgt taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct tttcgccatc
     1621 gtcattaagt ccggcttatc ggcgtaatga tccatggcaa acagtttacc ggtacgcgca
     1681 aagccgcttt gcacttcatc ggcaatcatc acaataccgt gctcgtcgca caggcggcga
     1741 atggcggcaa ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg caccggttcg
     1801 aaaataatgg ccgcgacctg cttcgcttcg atgtctgatt taaacaagcg ttcgatggcg
     1861 tcgagggagt cctgtgttga aatgccgtgt aaatctgacg gataaggtac gtgatacacc
     1921 gaaccaggga acgggccgaa gccgattttg tacggcgcaa cttttccggt cagcgccatg
     1981 gtcatatatg tacgaccgtg gaagccaccg ctaaacgcaa tcacgccagg tcgtccggta
     2041 tgggcgcgag caattttcac cgcgttttcc accgcttccg caccggtagt gaagaatgcg
     2101 gttttagcct gcccgctcac tggggcaagg gcgttaattt tctccgccag cgtgacgtag
     2161 ctttcgtacg gcacaatctg atacgcggtg tgggtaaact gttgcaattg ctgctccacc
     2221 gccgcgacca gatcagggtg gcgatgcccg gtattcagca ccgcaatgcc tgcggcgaaa
     2281 tcgatgtatt cgttgccctc tacgtccttc agcgtagcgt tttctgccga ctgggcgaag
     2341 aagttacaca tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg atggaattca
     2401 ttgttgctca tgtttcacac cttttgactc ttcactgtta atcggaaatt aaacgcccag
     2461 acgatcgcgc aggctgtaat aagccgcgcc catcgcggta aatggcacgc gcagcgtgcg
     2521 cccgccgggg aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt cggcgtcgcc
     2581 gcgcagcagt tcggcaatca gacgcccggc aagatgggta caggtgacgc cgtggccgct
     2641 gtagccctgc atgtaataga tgttggtatc aaggcgaccg aactgcggca tgcgcgacaa
     2701 ggtcagcagg aagttgcccg tccagcggta atcaattttc acgcccttca gctgcgggaa
     2761 ggttttcagc agtttcggca ccacaaggcg ctcaacgtca tccgggtcgc gcgcgccgta
     2821 gaccacgccg ccgccgtaca gcaggcggtt gtcggcggta agacggtagt aatcaagcag
     2881 atagttacag tcttccacac agtagttttt cgggatcagc gaacgggcta aatcttccga
     2941 cagccgttcg gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg ccagttccgg
     3001 ctctacttta tcgcccagat acgcattccc ggcgacaatc acatacttcg ccgtcacctg
     3061 acctttggca gttcgcacaa cggctggcgt ggtgtgctgg atctgcgtca cggcagaaag
     3121 ttcatacacg cgcccgccgt tgaggcggat ggcgtccgct tcgccaatgg cgaggtttag
     3181 cggatgaata tgcccaccgc tgtgatccag cagcgcaccg gtatagcgat cgctggctac
     3241 ttcacggcga atggcgttgg cgtccagcaa ttccagctgt ttattgccgt agcgttccca
     3301 gttctctttc tgctcttcaa gtgtggcgag ctgtttatca ttcatcgcca caaacaggcc
     3361 gccggggcgg tagtcgcagt caatctgata acgtttgata cgttcgcgga tgatctcacc
     3421 accttcgaac atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc ttttttcgat
     3481 cacgtcgatg tcgcggctgt aggagttcac aagctgcccg ccattgcgcc cgcttgcgcc
     3541 aaagccgatg cgtgaggctt cgagaaccac tacgtcaaag cccgcttccg ccagatgcag
     3601 cgcggaagag agcccggtat agccgccgcc gaccacgcaa acgtcgcagg aaatcgactc
     3661 attcagcgtg tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac tgctggtatg
     3721 ttcggtcatt tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa
     3781 gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg
     3841 tcatatcgcc gtcgttgtag ttattgacga agacggaacc agccttcagg cggcggctca
     3901 tgcggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg tactggctgt
     3961 cgttggcaag ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc agcaccggac
     4021 cgaaaatctc ttcgcggctt aaggacgcat tcgggtctac atccacaaag atggtcgggc
     4081 cgatggcggc agccagctca gcgttacgac catccagcag cagttgccct ttgctttcgc
     4141 cttcccgaat aaagctatgg accgagtcgg cgtgggcgca gtcgattaag gtgcccatgg
     4201 tggttgcggg atcaagtgga tggcccggct gccagttttg cgcctgctgt tttaacaggg
     4261 ctaagaattc atcggcgatg ctctcttcca gcaacaaacg cgttccggcg atgcacacct
     4321 ggccctggtt gtagaagatg cctgcggcgg tggcgcttgc cgctttttgc agatccggac
     4381 aatcggcgaa aacgaggttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt
     4441 tgctgtcgcc cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa
     4501 tggcgtcgat atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca
     4561 ccacgttcaa cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg
     4621 cactgagcgg tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg
     4681 ggccgagttt ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca
     4741 cgccgaccgg ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc
     4801 catacacttt gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat
     4861 catcacgcag actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct
     4921 cttcggcgtg ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg
     4981 gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt
     5041 cgatatcgac gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg accggatcaa
     5101 cggtttcaaa ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt
     5161 tttcaatggc gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta
     5221 tgactcctgt ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc
     5281 cccgggccgt atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag
     5341 ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg
     5401 attcgctcac ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc
     5461 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
     5521 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcggtatgcc ggtattaatg
     5581 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
     5641 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
     5701 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
     5761 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
     5821 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
     5881 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
     5941 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
     6001 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
     6061 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
     6121 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
     6181 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
     6241 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgc ccatgcagag
     6301 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
     6361 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
     6421 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
     6481 ccagttcctg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
     6541 taattaacgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
     6601 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
     6661 tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc atgtggtagc gcaattggca
     6721 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
     6781 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
     6841 tgctcaatct cacaaagtgg actaaattat cgccattact gcggtttaaa tgacctttat
     6901 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
     6961 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
     7021 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
     7081 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
     7141 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
     7201 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa gggtgttatt
     7261 tcccggcctc ggtgtttgca atggatattc tgggcaacgt agtagaagaa gcgggtctgg
     7321 gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt tctcggttcc ttaacccctt
     7381 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
     7441 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctacgccagc
     7501 gcggattgtt cccggttgta gcggtagagc tggagttcta tttactggat cgtcagcgcg
     7561 acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa
     7621 gccaggttta ctccgttgat aacctcaacc acttcgccga tgtgctcaat gatattgatg
     7681 aactggcgca gttacagtta attcccgccg acggtgcggt cgccgaagcc tcgccgggtc
     7741 agtttgaaat caacctttac cataccgata acgtgctgga agcctgcgat gatgcgctgg
     7801 cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg
     7861 cgaagccgta tgaagagcac gcgggcagcg gaatgcatat ccatatcagt atgcaaaata
     7921 accgtggcga gaacgtgctt tctgacgcgg aaggcgaaga ttcgccgctg ctgaaaaaga
     7981 tgctcgccgg gatgattgac ctgatgccgt catcaatggc gttgctggca ccaaacgtga
     8041 actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata
     8101 acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg tcataatcac cgcgtggaat
     8161 atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta
     8221 ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg
     8281 aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga
     8341 atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa
     8401 atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga
     8461 aaaacgcgta attccccatg cggggttgtt tcatcggcct gagcggagcc aggcctgaca
     8521 tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc
     8581 ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg
     8641 catggcgagg gtggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg
     8701 cttcccttgc taaacaggtt tgatttcagg ttgacacgac cgcaaacctt attacgcagg
     8761 ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac
     8821 ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac
     8881 gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc
     8941 ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact
     9001 ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaatcc
     9061 gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat
     9121 caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt
     9181 gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt
     9241 cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt
     9301 cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc
     9361 gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt
     9421 ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg
     9481 cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc
     9541 gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc
     9601 actgcctgaa attgcgctgt acgtcggcgg taagctgttc cagtcgattt tcctttgcac
     9661 cacgtttgtg aacacgttag cgtctggcct ggcctcacat gccagcgtgt cacgtctgct
     9721 gtatgtgatg gggcgcgaca atgtgtttcc ggagcgcgtg tttggctatg tgcatccgaa
     9781 atggcggact ccggcactga acgtcattat ggtcgggatt gtcgcgatgt cggcgctgtt
     9841 cttcgattta gttaccgcca cagcgttaat taacttcggc gcgttggtag cgtttacctt
     9901 cgttaacctg tcggtgttca accacttctg gcggcgcaaa ggaatgaata aaagctggaa
     9961 ggaacacttc cactatttat taatgccgct ggttggcgcg ctgacggtgg gcgtgctgtg
    10021 gattaacctc gaagccacct cgctgacgct gggtctggta tgggcttcgt taggcggcgc
    10081 atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac
    10141 gccagtgagc gaaacgtaat agtcggatgc gcgccgcatt atcctgcctg agggtaatgc
    10201 gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat catccgccct
    10261 tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt
    10321 ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca
    10381 caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca
    10441 gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg
    10501 agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg
    10561 tcagtatcgt tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg
    10621 cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg
    10681 tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg
    10741 ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat
    10801 gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg
    10861 tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg
    10921 aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag
    10981 tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc
    11041 agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg
    11101 ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg
    11161 ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg
    11221 tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca
    11281 acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg
    11341 ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg
    11401 caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc
    11461 gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc
    11521 gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa
    11581 tgccgcaggt agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga
    11641 ccggggaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg
    11701 acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca
    11761 ccgccaaacc gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata
    11821 accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac
    11881 cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat
    11941 cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca
    12001 cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata
    12061 tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc
    12121 ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc
    12181 cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg
    12241 cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc
    12301 tggcagcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta
    12361 ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg
    12421 tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg
    12481 tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt
    12541 cctgctgacc ttcgttggct tcagtttgag ctatttcact ccacacgcgc cgttacaagg
    12601 cgcgtcactg tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg
    12661 tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct tccctgcgac
    12721 gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat
    12781 gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttgct
    12841 gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac
    12901 gctaggttgg ttgcccgttt cagggcgttt cgatctgctg tacgaagtga aaccgattac
    12961 cgggtttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat
    13021 gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga
    13081 agtgatccga ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgttaaagc
    13141 ggcggctacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc
    13201 gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga cgctggcaat
    13261 gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg
    13321 ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat
    13381 tgttaacgtg atttctgata ttttgggtgc tatggctaac cctctgaaac ataaggaatg
    13441 gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct
    13501 ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg
    13561 ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg
    13621 gttaccaatt attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga
    13681 ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg
    13741 gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg
    13801 ccggggcgac gcacggccta cgctcagcgg tgcttaacca tattctggat accttactgg
    13861 cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc
    13921 acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca
    13981 tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa
    14041 cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga
    14101 tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg
    14161 gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt
    14221 atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg
    14281 ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt
    14341 actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt
    14401 cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg
    14461 ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt
    14521 tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg
    14581 gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga
    14641 cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg
    14701 ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt
    14761 ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga
    14821 atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga
    14881 cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg
    14941 tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag
    15001 ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag
    15061 taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc
    15121 agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc
    15181 actgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg
    15241 agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt
    15301 cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa
    15361 gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa gcggtaaaac
    15421 ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg
    15481 gtaaatccac gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat
    15541 tgattgacga tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca
    15601 tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg
    15661 attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg
    15721 aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg
    15781 cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca
    15841 ttattgccga tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc
    15901 tgatgctgga attacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg
    15961 gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgaac
    16021 gagggagcac cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg
    16081 ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg
    16141 aatcctcctt ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag
    16201 gtgatggata aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa
    16261 cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa
    16321 catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag
    16381 ctggatggtg ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt
    16441 gagtcgcaga gctggaccgt gctgcgtttc tggcataacg agattgattg taatgaggag
    16501 gcggtgctgg agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc
    16561 gagggggccg accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa
    16621 tattcggcaa ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag
    16681 cctgtttgtt gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag
    16741 agggttaggg taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctggc
    16801 aactatagct actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta
    16861 ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc
    16921 tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat
    16981 cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca
    17041 gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt
    17101 tctgcagtgc gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa
    17161 agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct
    17221 ggatggtgac tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag
    17281 ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc
    17341 cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg
    17401 tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga
    17461 aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat
    17521 caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt
    17581 tactattgaa gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat
    17641 ctccggtgaa gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga
    17701 attgaaataa tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt
    17761 tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca
    17821 ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa
    17881 aagccactgg tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt
    17941 cccggaagcc gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac
    18001 gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc
    18061 tgaccagctt accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc
    18121 cctgacggtt gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact
    18181 tacgccgctc cccgggcctg attcctccag ggaaagcaat cataggttgg cacaaatcac
    18241 gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca
    18301 tctgaatgac gtcggtctta ctgccgggga ctgtgtgatc attaaccaaa tcattggttt
    18361 tattggcttt caggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg
    18421 ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc
    18481 aatacgctgg cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc
    18541 aacagcagaa ctttgccaac tggccgaaac actctctctc caccctattt cactttccct
    18601 tctcgaaagg ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc
    18661 gttgttatgc gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc
    18721 aaatgaatat aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg
    18781 ggctgaccgt cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc
    18841 acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac
    18901 gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat
    18961 cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc
    19021 cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc
    19081 ttaaacagca actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa
    19141 aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga
    19201 tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg
    19261 aatccgcaga gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc
    19321 agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc
    19381 cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg
    19441 aaatgcgccg tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca
    19501 tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg
    19561 aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag
    19621 atctgaccgc gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg
    19681 cccttttcgc taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc
    19741 caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca
    19801 ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct
    19861 gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg
    19921 atggcaccat tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc
    19981 tggtgtatga ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg
    20041 aagcaattgc cgaacaagtc cgtttgttag cgcaaatttg ccaacgccgc ggcgagtggc
    20101 gtcataacca cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa
    20161 aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag
    20221 aagcgatgat tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg
    20281 gcatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc
    20341 tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca
    20401 aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct
    20461 tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag
    20521 catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc
    20581 tgaaagcggt tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa
    20641 tggccgagcg tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg
    20701 cacgcttcct gaaagacaaa gccgggaccg acacccgttt cgcggcggaa attgtcgata
    20761 tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg
    20821 cacctttctt acacgctatg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac
    20881 aaattaaagg tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag
    20941 tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc
    21001 attcctcatg gatgggcc
//
LOCUS       DAPPNJ010000070        20376 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000070 DAPPNJ010000000
VERSION     DAPPNJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.070"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            82..897
                     /gene="mepH"
                     /locus_tag="RPM73_002130"
     CDS             82..897
                     /gene="mepH"
                     /locus_tag="RPM73_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDX6520488.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1025..1606
                     /gene="sodB"
                     /locus_tag="RPM73_002131"
     CDS             1025..1606
                     /gene="sodB"
                     /locus_tag="RPM73_002131"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDX6520489.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1841..3010)
                     /locus_tag="RPM73_002132"
     CDS             complement(1841..3010)
                     /locus_tag="RPM73_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6520490.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3176..3265)
                     /gene="ynhF"
                     /locus_tag="RPM73_002133"
     CDS             complement(3176..3265)
                     /gene="ynhF"
                     /locus_tag="RPM73_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDX6520491.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3564..4589
                     /gene="purR"
                     /locus_tag="RPM73_002134"
     CDS             3564..4589
                     /gene="purR"
                     /locus_tag="RPM73_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDX6520492.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4586..5518)
                     /locus_tag="RPM73_002135"
     CDS             complement(4586..5518)
                     /locus_tag="RPM73_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520493.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5631..6842
                     /locus_tag="RPM73_002136"
     CDS             5631..6842
                     /locus_tag="RPM73_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDX6520494.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            7133..8281
                     /gene="cfa"
                     /locus_tag="RPM73_002137"
     CDS             7133..8281
                     /gene="cfa"
                     /locus_tag="RPM73_002137"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDX6520495.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8321..8962)
                     /gene="ribE"
                     /locus_tag="RPM73_002138"
     CDS             complement(8321..8962)
                     /gene="ribE"
                     /locus_tag="RPM73_002138"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDX6520496.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9177..10550
                     /gene="mdtK"
                     /locus_tag="RPM73_002139"
     CDS             9177..10550
                     /gene="mdtK"
                     /locus_tag="RPM73_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDX6520497.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10591..11847)
                     /locus_tag="RPM73_002140"
     CDS             complement(10591..11847)
                     /locus_tag="RPM73_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX6520498.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12155..12231
                     /locus_tag="RPM73_002141"
     tRNA            12155..12231
                     /locus_tag="RPM73_002141"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12189..12191,aa:Val,seq:gac)
     gene            12236..12312
                     /locus_tag="RPM73_002142"
     tRNA            12236..12312
                     /locus_tag="RPM73_002142"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12270..12272,aa:Val,seq:gac)
     gene            12420..12725
                     /locus_tag="RPM73_002143"
     CDS             12420..12725
                     /locus_tag="RPM73_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDX6520499.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12851..14455
                     /locus_tag="RPM73_002144"
     CDS             12851..14455
                     /locus_tag="RPM73_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDX6520500.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14467..15279)
                     /locus_tag="RPM73_002145"
     CDS             complement(14467..15279)
                     /locus_tag="RPM73_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520501.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15283..16068)
                     /locus_tag="RPM73_002146"
     CDS             complement(15283..16068)
                     /locus_tag="RPM73_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDX6520502.1"
                     /translation="MNPSQHAEQFQRQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16065..16733)
                     /locus_tag="RPM73_002147"
     CDS             complement(16065..16733)
                     /locus_tag="RPM73_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDX6520503.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVRACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16797..17435)
                     /locus_tag="RPM73_002148"
     CDS             complement(16797..17435)
                     /locus_tag="RPM73_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDX6520504.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17448..19550)
                     /locus_tag="RPM73_002149"
     CDS             complement(17448..19550)
                     /locus_tag="RPM73_002149"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDX6520505.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19571..20197)
                     /locus_tag="RPM73_002150"
     CDS             complement(19571..20197)
                     /locus_tag="RPM73_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDX6520506.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         5079 a         5326 c         4777 g         5194 t
ORIGIN      
        1 tcttcttcta tgcattagaa tcatcaagtt ttgtaaatca gacgcaggca tgatagacct
       61 gccttaacag agggacgctc agtggcgcgg ataaaccgta tttcgatcac gctctgtgct
      121 ttacttttta ccaccctgcc tttaacgcct atggcccatg cttcaaagca agccagggag
      181 agttctgcta ccactcatat caccaaaaaa gcagataaaa agaaaagcac ggcaaccacc
      241 aaaaagaccc agaaaacagc gaaaaaagcc gccagtaaaa gtacgaccaa aagcaaaacc
      301 gcttcttccg ttaaaaaatc ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa
      361 cacgccgtca ataaaacggc ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc
      421 tataaatcgc attgtgtgaa agtcaaaaat gccgcgtcag gaactcttgc cgacgcgcac
      481 aaagcgaagg tgcaaaaagc tacgaaagtg gcaatgaata aactgatgca gcaaattggt
      541 aagccatatc gttggggtgg cagctcaccg cgtaccggtt ttgattgcag cggcctggtt
      601 tattacgctt ataaagattt ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat
      661 cacctgcgtg atgcagcgcc aatcgaacgt agtgaactga aaaacggcga cctggtcttt
      721 ttccgtactc agggacgcgg cacagccgat catgtcggcg tgtatgtcgg caacggcaaa
      781 tttattcagt caccgcgcac aggtcaggaa attcaaatca cttctctcag tgaagactac
      841 tggcagcgcc actatgttgg cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac
      901 tttaccctgt tgttacggca acagggtaag ttcatctttt gtctcacctt ttaatttgct
      961 accctatcca tacgcacaat aaggctattg tacgtatgca aattaataat aaaggagagt
     1021 agcaatgtca ttcgaattac ctgcactacc atatgctaaa gatgctctgg caccgcacat
     1081 ttctgcggaa accatcgagt atcactacgg caagcaccat cagacttatg tcactaacct
     1141 gaacaacctg attaaaggta ccgcgtttga aggtaaatca ctggaagaga ttattcgcag
     1201 ctctgaaggt ggcgtattca acaacgcagc tcaggtctgg aaccatactt tctactggaa
     1261 ctgcctggca ccgaacgccg gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc
     1321 atcttttggc agctttgccg atttcaaagc gcagtttact gatgcagcga tcaaaaactt
     1381 tggttctggc tggacctggc tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac
     1441 ctctaacgcg ggtactccgc tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg
     1501 ggaacacgct tattacatcg actatcgcaa tgcacgtcct ggctatctgg agcacttctg
     1561 ggcgctggtg aactgggaat tcgtagcgaa aaatctcgct gcataataac tgatggcaaa
     1621 tgcagcattg cctgaagcgc tacgcttatc aggcctacgc ggatcatcga tgtaggtcgg
     1681 ataaggcact cgccgcatcc gacaagcgct gcatgatacg acgcctaacg cgtcttttca
     1741 tgtcaacgcg gatgagtcgt taacaccgca tccggcaaga taaatcgcac gttgtcagca
     1801 actgtaacgc agaaggttat ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa
     1861 ctgtttcagg ttgttttctg gctgacataa acaccagcaa taatgccagt cccgcgacaa
     1921 tcgctcccat caccggcaca aagctgtatc ccagcccagc ggaaattacc gcaccaccag
     1981 cagctgctcc cagcgcattt ccaagattaa aggcaccaat attgactgat gaagacagac
     2041 ccggcgcttc actggcgaca cgcatcacgc gcatctgtaa cggcggtacg accgcaaagg
     2101 ttgctgcgcc ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg
     2161 ggattgccag cataatcacc atcagcagca acaaaaagcc tttcaacgtg ccgttaactg
     2221 aacgatctgc cagtttgccg ccgagatagt taccgataga gaatccgaca ccaatcagca
     2281 ccagcattgc cgtgacgaac actggtgttg cgtgggtaat actttgcagt accggagaga
     2341 tataggtgta gagagtaaac attgcaccag ctcccagtac cgtcgtcagc aatgcagaca
     2401 gcacctgcgg acgcattaat accgccagct cttttttcac ttcaggtcgt gcccctgcac
     2461 cacctttagg taatgagaag aacagactta ccattgaaat cactcccagc cccgccgttg
     2521 ccagaaatga catccgccag ccgatggttt cacccaacca ggtcgccgcc ggcacgccac
     2581 cgatatttgc cagggttaac cccataaaca tagtggcaac tgcgctggcc tgtttatgtt
     2641 ttggcaccac gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg tgattcaggc
     2701 tggtcaaaat gcgtgaaagc atcagggtca tataatccgg cgcgatggcg gaaagaacgt
     2761 tgcctagcgt gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa
     2821 gtagaagcgt catcagcggc gcgcccacca ttacgccaac tgcataggca ctgattaaca
     2881 ttccggcagc gggaatcgag acatccacac cgcgcgcaat gacgggcaac aagcccattg
     2941 gcgagaactc cgttgtcccg ataccaaacg cgccaatcgc cagcgccagc aacggatagt
     3001 taattttcat gccttatctc cacctcttcg cgtcattacg cgatattcat taaagtggcg
     3061 aaagcatgac agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca cttttgccat
     3121 tttgctaaca aacaggaagg agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt
     3181 gcagcgcggc agtcaaaccc acggctacga tcaaaccgag gacgataatc gttgttacca
     3241 gtgaaaattt aaggtcggtg ctcatcaagt tttctccttt tttattacca cacaaaaagt
     3301 gatattacgc atttttacac actgtgatga aaaaatctcc cgtcatttat aatgataagt
     3361 gtttttacca cttccccttt tcgtcaagat cggccaaaat tccacgctta cactatttgc
     3421 gtactggcca ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta ccgcaacact
     3481 tttgttgtgc gtaaggtgtg taaaggcaaa cgtttacctt gcgattttgc aggagctgaa
     3541 gttagggtct ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa cgagcaaacg
     3601 tttccactac aactgtgtca cacgtgatca acaaaacacg tttcgtcgct gaagaaacgc
     3661 gcaacgccgt gtgggcagcg attaaagaat tacactactc ccctagcgcg gtggcgcgta
     3721 gcctgaaggt taaccacacc aagtctatcg gtttgctggc gaccagcagc gaagcggcct
     3781 attttgccga gatcattgaa gcggttgaaa aaaattgctt ccagaaaggt tacaccctga
     3841 ttctgggtaa tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg atgatggcgc
     3901 aaaaacgcgt cgatggtctg ctggtgatgt gttctgagta cccagagccg ttgctggcga
     3961 tgctggaaga gtatcgccat atcccaatgg tggtgatgga ctggggtgaa gcaaaagctg
     4021 acttcaccga tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc gggcgttatc
     4081 tgattgaacg cggtcaccgc gaaatcggcg ttatccccgg cccgctggaa cgtaacactg
     4141 gcgcaggccg ccttgccggt tttatgaagg cgatggaaga agcaatgatc aaggtgccgg
     4201 aaagctggat tgtgcagggt gattttgaac ctgaatccgg ttatcgcgcc atgcagcaaa
     4261 tcctgtcgca gccgcatcgc cctactgccg tcttctgtgg tggcgatatc atggcaatgg
     4321 gcgcactttg tgctgctgat gaaatgggcc tgcgcgtccc gcaggatgtt tcgctgatcg
     4381 gttatgataa cgtgcgcaac gcccgctatt ttacgccggc gctgaccacg atccatcagc
     4441 caaaagactc gctgggtgaa acagcgttca acatgctgtt ggatcgtatc gtcaacaaac
     4501 gtgaagaacc gcagtccatt gaagtgcatc cgcgcttgat tgaacgccgc tccgtggctg
     4561 acggcccgtt ccgcgactat cgtcgttaat cacccgttgc gggagtctct tccggctccc
     4621 gcagccactc cttattcagc gtctcactat cgccgagata ctcaagcaac caggttaacg
     4681 caggcgacat atcattttgc tgccatgtca gacaacatgc cgaatccgga aaggggtttt
     4741 ccagttctaa tgctacccac ttcccctcat taagccacgg tttggcgaaa tgtgttggca
     4801 ccatccctat gcataatcct gccgagatgc aggttgccga tgattcccag tcaggcacga
     4861 caagtctttt ttggttatcc agcaaccagg taatacgctt aggtagcgtt cgcgaggtgt
     4921 cttcgcgcac caacgacggc cagttgcgca acgtatcatc gctgaacggg ccatccatca
     4981 acgccagcgg gtgatggctg gcaacaacgc aactccagct tagcatcccc atatcccgga
     5041 aggcataacg accgcctacc ggaatcgcgc gtgttgcgcc aatcgccagt tccacgcgcc
     5101 cgtcggaaag cgcatcccag acaccgttga acacttcctg aaagacaaga agttcgacat
     5161 catcaaaatg gcgataaaaa tcaacgatca tctgccgtgt acgttctggc ctgacaatat
     5221 tatccactgc gatagctaac tgaccgcgcc agccgttcgc tatctgctga cattgctggc
     5281 gggtgatctg catttttttg acaacagagc gcccttcttt gagaaaccac gctccggcag
     5341 cggtcagttc cacatcacgg tgccgtcttt caaagagcgg caccgccagc cactcttcca
     5401 gctgacgcac ggtatagctg accgcagaag gaacgcgatg caactcctgt gccgcagcgc
     5461 taaaactacc attacgcgct accgcatcaa caacttcgag tgaatattct gaccacatag
     5521 tctgcctgca aaatttttga aaccagtcat caaatattac cgtttcacaa cactaatttc
     5581 actccctaca ctttgcggcg gtgtttaatt gagagattta gagaatatac atgcaacctg
     5641 ggaaaagatt tttagtctgg ctggcgggtt tgagcgtact cggttttctg gcaaccgata
     5701 tgtatctgcc tgctttcgcc gccatacagg ccgacctgca aacgcctgcg tctgctgtca
     5761 gtgccagcct tagtctgttc cttgccggtt ttgccgcagc ccagcttctg tgggggccac
     5821 tctccgaccg ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt tttgcgttag
     5881 gtagtctggg gatgctgtgg gtagaaaacg ccgctacgct gctggtattg cgttttgtac
     5941 aggctgtggg ggtctgcgcc gcggcggtta tctggcaagc gttagtgacg gattattatc
     6001 cttcacagaa agttaaccga atttttgcga ccatcatgcc gctggtgggt ctatctccgg
     6061 ccctggctcc tctgttagga agctggctgc tggtccattt ttcctggcag gcgattttcg
     6121 ccaccctgtt tgccattacc gtggtgctga ttctgcctat tttctggctc aaacccacga
     6181 cgaaggcccg taacaatagt caggatggtc tgacctttac cgacctgcta cgttctaaaa
     6241 cctatcgcgg caacgtgctg atatacgcgg cctgttcagc cagttttttt gcatggctga
     6301 ccggttcacc gttcatcctt agtgaaatgg gctacagccc ggcagttatt ggtttaagtt
     6361 atgtcccgca aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc gcgctgcaga
     6421 aatggcaagg caagcagtta ttaccgtggt tgctggtgct gtttgcagtc agcgtcattg
     6481 cgacctgggc tgcaggcttc attagccatg tgtcgctggt cgaaatcctg atcccattct
     6541 gtgtgatggc gattgccaat ggcgcgatct accctattgt tgtcgcccag gcgctgcgtc
     6601 ccttcccaca cgcaactggt cgcgccgcag cgttgcagaa cactctacaa ctgggtctgt
     6661 gcttcctcgc aagtctggta gtttcctggc ttattagtat cagcacgcca ttgctcacca
     6721 ccaccagcgt gatgttatca acagtagtgc tggtcgcgct gggttacatg atgcaacgtt
     6781 gtgaagaagt tggctgccag aatcatggca atgccgaagt cgctcatagc gaatcacact
     6841 gatctatatc gatatactta tacttaggct gctaacaaaa ttttgttgta tgattgaaat
     6901 tagcggccta tactaatttc gagttgttaa agctacgata aatattatgt ttttacgggg
     6961 acaggatcgt tcccgactca ctatggatgg tcatttcggc aagggttcct cctttccctc
     7021 tgttctacgt cggattatag actcgcggtt ttttctgcga gatttctcac aaagcccaaa
     7081 aagcgtctac gctgttttaa ggttctgatc accgaccagt gatggagaaa ctatgagttc
     7141 atcgtgtata gaagaagtca gtgtaccgga tgacaactgg taccgtatcg ccaacgaatt
     7201 acttagccgt gccggtatag ccattaacgg ttctgccccg gcggatattc gtgtgaaaaa
     7261 ccccgatttt tttaaacgcg ttctgcaaga aggctctttg gggttaggcg aaagttatat
     7321 ggatggctgg tgggaatgtg accgactgga tatgtttttt agcaaagtct tacgcgcagg
     7381 tctcgagaac caactccccc atcatttcaa agacacgctg cgtattgccg gcgctcgtct
     7441 cttcaatctg cagagtaaaa aacgtgcctg gatagtcggc aaagagcatt acgatttggg
     7501 taatgacttg ttcagccgca tgcttgatcc cttcatgcaa tattcctgcg cttactggaa
     7561 agatgccgat aatctggaat ctgcccagca ggcgaagctc aaaatgattt gtgaaaaatt
     7621 gcagttaaaa ccagggatgc gcgtactgga tattggctgc ggctggggcg gactggcgca
     7681 ctacatggca tctaactatg acgtaagcgt ggtcggcgtc accatttctg ccgaacagca
     7741 aaaaatggct caggaacgct gtgaaggcct ggatgtcacc attttgctgc aagattatcg
     7801 tgacctgaac gaccagtttg atcgtattgt ttctgtgggg atgttcgagc acgtcggacc
     7861 gaaaaattac gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg aaggtatatt
     7921 cctgctccat actatcggtt cgaaaaaaac cgatctgaat gttgatccct ggattaataa
     7981 atatattttt ccgaacggtt gcctgccctc tgtacgccag attgctcaat ccagcgaacc
     8041 ccacttcgtg atggaagact ggcataactt cggtgctgat tacgatacta cgttgatggc
     8101 gtggtatgaa cgattcctcg ccgcatggcc agaaattgcg gataactaca gtgaacgctt
     8161 taaacgaatg tttacctatt atctgaatgc ctgtgcaggt gctttccgcg cccgtgatat
     8221 tcagctctgg caggtcgtgt tctcacgcgg tgttgaaaac ggccttcgag tcgctcgcta
     8281 aaggctattc tatcgccccc tctccggggg cgatttcaga tcaggcttct gtacctggtt
     8341 gattcatggc attttctcgt gccgccagca cacgttctac cgtatctacc actgcctgag
     8401 tttgtggatc aatttcaatg ttgacgcgtg cgccgagttt tttcttccca agagtcgtgc
     8461 gttccagagt ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact tcgccgacgg
     8521 tcaggctaat accgtcgatg ccaataaatc ctttgtacag gatatatttc atcaactgac
     8581 tatcctggac tttaaaccag atctggcgat tattttccga ggttaatatt ttcgccactt
     8641 cagctgtggt cataatatga cctgacatta agtgtccgcc aatttcatca ctgaatttcg
     8701 ccgcacgctc aacgtttacc caatccccca cttttaaatc gccaagattg gtaatgcgta
     8761 acgtttcttt catcaggtca aaactgacat ggttgccgtt aatttccgtc acggtcaggc
     8821 agcaaccgtt atgcgccacg gaagcacctg tttccaggcc gtccagcatg tggtcgggta
     8881 actccaccac atgcgtacga aaatttggtt tctcgtcaat cgacaccagt tttgcggtgc
     8941 cctgtacaat ccccgtaaac atacttacaa ctcctgaaat cagttaagac attctgttca
     9001 gcacaatagc aggtggcaaa cgcccttacc agtgaagggg taagaatgac tattttttca
     9061 ctggagaatt aataaatcct cgctacaata gactgaattt cccctgcttc ttctttttgc
     9121 tgcccattca ggcggctttt tagtctctca tataactaca aataaaaggt gttcacgtgc
     9181 agaagtatat cagtgaagcg cgtctgttat tagcattagc aatcccggtg attctcgcgc
     9241 aaatcgccca aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc tatagtgcca
     9301 ccgacatggc ggcggtcgct atcggtactt ctatctggct tccggcgatc ctctttggtc
     9361 acggactgct gctggcatta acgccggtta tcgcgcaatt aaatggttcc ggtcgacgtg
     9421 agcgcattgc gcatcaggtg cgacaaggtt tctggctggc aggttttgtt tccgttctca
     9481 ttatgcttgt gctgtggaat gcaggttaca ttatccgctc catggaaaac atcgatccgg
     9541 ctctggcgga caaagccgtg ggttatctgc gtgcgttgtt gtggggcgcg ccgggctatc
     9601 tgttcttcca ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag ccgggtatgg
     9661 taatgggctt tatcggcctg ctggtgaaca ttccggtgaa ctatatcttt atttatggtc
     9721 atttcggtat gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg gcggtgtatt
     9781 gggtcatgtt ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg cgcgatattc
     9841 gtaacgaaaa aggcaccgcg aaacccgatc ctgcggttat gaaacgactg attcaactcg
     9901 gtttgccgat tgcgctggca ctattctttg aagtgacact gtttgccgtc gtggctctgt
     9961 tagtgtctcc gctcggtatt gttgatgtcg caggacacca gattgccctg aactttagtt
    10021 cactgatgtt tgtgcttcca atgtcgctgg cggcagcggt aactatccgc gtaggttatc
    10081 gtctgggtca gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg cttatggtgg
    10141 gtgtctgtat ggcaaccctg acggccatat tcacggtttc actgcgggag caaatcgccc
    10201 tgttgtacaa cgacaatccc gaggttgtaa cgctggctgc gcatttgatg ttgctggcgg
    10261 cggtatatca gatttctgac tcaatccagg tgattggcag tgggattttg cgtggttata
    10321 aagatacgcg ttccattttc tatatcacct ttacggctta ctgggtgctg ggcttgccaa
    10381 gcggctatat tctggcactg accgatctgg tcgttgaacc tatggggcca gcaggcttct
    10441 ggataggctt tattattggc ctgacgtcgg cagccattat gatgatgctg cgtatgcggt
    10501 tcctgcaacg tctgccgtca gttatcattc tgcaacgagc atcccgctaa taaagacaag
    10561 gcgcaacctt cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg cgccaatccc
    10621 gtcttcgtct ggctgtaatt tcagagcgtt gcacagagtt tgccgactgg catctgttat
    10681 ctcaacgtgt atatccccgg taacttcccc tttcacattg atgaagtcag gtgagatttc
    10741 tttcgcactg gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca ctttcagttg
    10801 cacatgcgtt gccgcaccag tgatcagcca cagttttccg ccattcagat tgatgttacc
    10861 aacaataaaa gtgacaccgt caccttttac cgtactgccg tcgagaatca acacaccgcc
    10921 ctgctgtaca gtaatcgttg attgttgcag atctgcgcca tagccaataa tcattgaagc
    10981 gccgtccagt aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt cctctacgac
    11041 aaaattttcg tccgcgctgg tgggtgacaa caggatcact gttccacctt gtattaccgc
    11101 attttccagc gtaccggcat gcacttcagc ccgaccaccc acattcacgg acaggttttg
    11161 cgtcttaccg gaactgtaga gctgaaggcc atccgtcccc agacgataaa tggatccatt
    11221 ctcaacaata gtgtcagagt cttcaccacc gagattattc atggtgccac cagcaagcaa
    11281 cgtatcactg gctttcccgg ccagcataaa aacgccgccc tgattaatct cacaattgct
    11341 ggcttcccca cctggataca ccaacaattc accaccttca tctaccacga ccgctgaggt
    11401 tgtcccatta accaacaggc cgccctcttg atcgagaatg attttttccg cgcgatgtcc
    11461 ttccagtaca cgcaggttac cgccattttc cagcaacaaa ccgcaggcat aaccctgatc
    11521 aacgctgaat tcaccttcgg gatggcggcc attaaccgta gcgagcgtag agagactaat
    11581 tgcgcctccc tcggactgaa caatatctgt ggcaatggca ccgtcgctga ccgatagcgt
    11641 gccattgtta ccgtactgtg tgcatttggc gatcccaccg gttgccactt tcaaccagcc
    11701 agcccccgtg accacggtat tttcggctct tccgccagct tcaacaatca actcgccttc
    11761 agtaagttgc gtcgcgccaa tgacctcgcc ggtcttaaca atttgcacat tcccgtcgct
    11821 catcaagttt ttcgcatcag atcccatgat ttattccttt gctgcatctg tgtgccttta
    11881 ttgctaccta agtgtaaagg ctacggagga tttatccacg acagatttga gatggtggca
    11941 aacaactctg tttaaactct gatacacgaa ttattgggtt gtatcagatg taaatgcgat
    12001 cctgaataaa aatcgccctt gcaaatcaac aaaatatagc caatttgctt aaaagctctg
    12061 caaacggtcg aaatagcgca gaaaattacg ttttgcctct tgccaccttc ccactctgcc
    12121 gctaatattc gtccccgttg tcacctacaa cgttgcgttc atagctcagt tggttagagc
    12181 accaccttga catggtgggg gtcgttggtt cgagtccaat tgaacgcacc atcctgcgtc
    12241 cgtagctcag ttggttagag caccaccttg acatggtggg ggtcggtggt tcgagtccac
    12301 tcggacgcac cagattttct taatctggtc ttctcctttt tccctctgtt tcttctctgt
    12361 atccaatacg ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg agaatcgcta
    12421 tggcaacttt gttacaactt cattttgctt ttaatggccc atttggcgac gcaatggctg
    12481 agcagcttaa accgcttgct gagtcgatta atcaggaacc tggttttctg tggaaggtat
    12541 ggacagaaag tgaaaagaac cacgaagccg gtgggatcta tctttttact gatgaaaaaa
    12601 gcgcccttgc ctatctggaa aaacatactg cccgacttaa aaatctcggc gttgaggaag
    12661 ttgtcgccaa agttttcgat gtcaatgagc cactgagtca aatcaatcag gcaaaactcg
    12721 cctgacagaa tttaatcaag ggcggttagc gcccttttca tccctgtctg aaatttctca
    12781 aattctaaaa atctcaacca aacttatctg ataacactaa attcgaaaga atgcgtacag
    12841 gtaagtaaca atgaaaaaaa ttgctattgt gggtgccggg cctacgggga tctacacctt
    12901 attctcgctt ctacagcaac aaactccact ttctatttct atcttcgagc aggctgacga
    12961 ggccggtgtc gggatgccat acagtgatga ggaaaactca aaaatgatgc tggcaaatat
    13021 tgccagtatt gaaataccgc cgattaattg tacgtatctc gaatggctac aaaagcaaga
    13081 agccagccat ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc gtcagttttt
    13141 accgcgaatt ctgctgggcg aatatttccg cgatcaattt ttacgattag tagaccaggc
    13201 acgacagcaa aaatttgcag tggctgttta tgaatcatgc caggttaccg atctgcaaat
    13261 tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga cgtttgattt
    13321 agcggtgatt gccacgggtc acgtctggcc tgatgaagaa gaagcaacca gaacgtattt
    13381 tcccagtccg tggtcaggcc tgatggaagc aaaggtcgat gcgtgtaacg tgggtattat
    13441 gggaacatcc ttgagcggac tggatgcggc aatggcagtg gctattcagc atggttcgtt
    13501 cattgaagat gataaacaac acgtcgtttt taaccgcgat aacgcaagtg aaaagctaaa
    13561 tattacgttg atgtcgcgca cgggtatttt acccgaagcc gatttctatt gccctattcc
    13621 ctacgagccc ttacacatcg tcaccgatca ggcattaaat gctgagattc aaaaaggtga
    13681 agagggcctt ttggatcggg tatttagatt gatagtagag gaaatcaagt ttgctgatcc
    13741 agactggagc caacgcatag ccttagagag cctgaatgtc gattcctttg ctcaagcctg
    13801 gtttgccgag cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc tccaggaagt
    13861 cgaacgcaat aaacgagaaa aacatactgt tccctggcgt tatgtcattc tgcgcctgca
    13921 tgaagccgta caggaaattg ttcctcatct gaatgaacac gaccataaac ggttcagtaa
    13981 aggccttgcc agggttttca tcgataatta tgcggcaatc ccttcagagt ctattcgtcg
    14041 cctacttgcc ttacgtgaag cgggaatcat tcatattctc gccctcggtg aagactacaa
    14101 aatggaaatt aatgagtcgc gcaccgtcct gaaaacggaa gacaacagct actcgtttga
    14161 cgtttttatt gatgcccgcg gacagcgtcc gcttaaagtg aaagatattc ctttccctgg
    14221 gctacgcgag caattacaga aaacagggga tgaaatccct gatgttggcg aagattatac
    14281 gttacagcaa cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc cctggttgat
    14341 gcacgaccag cctttcgttc agggacttac ggcatgtgca gaaattggtg aggcgatggc
    14401 tcgggcggtc gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg attaaaggta
    14461 aagctatcag cactcaacaa actgatgctg ttcattaaga cgatagcgga ctccggttcg
    14521 aatattgttt tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt catgatatgc
    14581 cagtgcggcg cttccccctg gcccgagaat aattgagtcg accacgcccg tgctcactac
    14641 cgttgaattt tcaccgctgg caatgatgtg tacattgtcg ccgctgttgg caattctggc
    14701 gtttgcgcca aagctggcga tttgcaccag gtcaccatta ctggcgacat gacaccgttc
    14761 acccaatgta caaacgcgta ctcgcattcc ggtattcgca atccgactac tatctccagc
    14821 gctgcttatc cgcgaagaat taccggttac agcaacccgt gcgcgttcac cggagctgcc
    14881 aatgtgactg ttaaagccga cacttccaat ccgcacgcta taacccatac tggcaatttg
    14941 cgcggcatac ccagcggttg cgattcgggc gttatcctca caacagccga tgcgagcatg
    15001 atcgtcgctg cgggcaaact gactgcaaaa tggtatctct ccggggtctg tggccagttt
    15061 ttgcatagag gaaacttcct gatgcgcgaa gttttcatcc gccagccatt ttttccacgc
    15121 atactcaacc agactctccg cccagtccgt atatccttcc tcaatcagtg catgatgaat
    15181 atcggcataa ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca tcaccgctcc
    15241 gatacgccat tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc ggtgatagcc
    15301 atcgaccatg cttttaaagg tttcgtgtgg cgtacgcccc gtggtgcaaa gataaagatg
    15361 accgaagata aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca ataaccagta
    15421 tcttacgcca ggaaacacat ctcccacggc ttgcggatag agacacaaca gccccgtcaa
    15481 tagtaacaac ggcagcaatc catacatgac accaacatag gcgacctgct gtaagggatt
    15541 aaatttagac tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa tgccaaacaa
    15601 ataaaatcgc gtttgttttg ccgctcgttc cagccacccc tgacgacgaa tgcgatagtg
    15661 gtgaccatta tccccaacgg cattgatcag cacaaagccg agccagcatg ccagtaacaa
    15721 aaatccgcaa acttcatgca ccgcaaccag acttttaacc gcagttgcgc ccaccatcgc
    15781 aaagtgattt atcagcccac ttgccagcaa caatacaaag agtaacgcat tcgaccagtg
    15841 ccataatctg accgctttgc tgtaaagata aaccttctca ccatgatcgg tggctgattt
    15901 cttcacgcca cgagcacgaa gcaaggcgtg cagccccagc accaaccaca tcccaaccag
    15961 caatactccg gcaatgatca accacaccgg ccaaaattcg ggagtgaact gtggcacata
    16021 gttcgctaac tgtctctgaa actgttcagc atgttgcgac gggttcatac attttccttt
    16081 ttaatcaaat gttgaccgaa ccggcgatac agatgcggtt tcccggcacc aggtagctga
    16141 tattgatagt atttattatc ctgcaaccac gcctgaattt ctgggctatc ttcgcgacca
    16201 aagatcaatg cgtgttccgg acaggcgcgg acgcaaatgg gcggaaagcc ttttgccagt
    16261 cgggactcag cgcagaaatc acatttatct gccactttag tgacagggtt aaggtagcga
    16321 acctggtacg gacaagcgcc aatgcaataa ctgcagccga tgcactgcga tttttctacc
    16381 cgcacgatcc cctgctcatc acgccacgac gcccctgtcg ggcatacgtc gatgcacgga
    16441 gcatcttcgc agtgctgaca tgactggcga aaaaagtgat actgcgtttc gttgtcgtta
    16501 tcggtgactg gaatgtgtgc tatcgataaa cggcttccct gagcggggac gtggttagtt
    16561 ttacggcatg cgcgggcgca aatattacag ccattgcacc gtgactcgtc atgaatcatg
    16621 gcataccgaa cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc agaaccagta
    16681 aaaaagatta ctgttcccat ccccagaaca aatttgcgtc gagtgaagga catctgatta
    16741 tcccctaatg cagtggctgc aactgtccac cctatccatg acatctccct gaaatttcag
    16801 gggttctgaa actgctctac ctcgaaccat tcgcataagc cataagcctt gccataggtc
    16861 ataaatcgct cgctaaacaa gtacaacacc gccgtcgatg actccacctg ccgtatatcg
    16921 gcatattcag gtgcaacatc catcgcggcg atcgcggctt caatctgcgc ttcctggaag
    16981 taatatggcg cttgcatcag catggcgact ttgtaaggac gaggatatgt ctggcactca
    17041 aaacgaacgg cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg caaagacatc
    17101 gccgcgtagt tttcactcat ggcctgcgtt gagtaatagt aatcatcctg gcttcccgtc
    17161 atacaagcga tatcagcgta gtctccgttc tgcttaatgc tttccagcaa aactgacagc
    17221 tcctcttctg caaccgaata gggtggcgag agaaacacgg cgcgcgccac cagctgccca
    17281 gccgcagaat gctggcgaat gaactctgcc aggatttcag ccggttctgg ttcggttacc
    17341 gtatcaacgg ggtgacgcat cccctgtaac cattgccgct tcgcctcgtc aacttcgctg
    17401 actttcgcta acggcaattc atcccgatgg ttcatctcac ccattcctta cacaggcagt
    17461 agattgtgtg ctgccagatc ggcggcaata tcttccagcc ccagacgctg caatgtttcg
    17521 cgggtgggac aaccaagctg tgggtcccag cccatttctt tgtagaacat cgtgagcgaa
    17581 gcatgcatat cgtcacgatc cattttgtca gtaccttcag taaagaccgg gatctgcgga
    17641 tccttgtcga atacccagga acagataaga tcgtgttcat tacgcatatc tttggtttgc
    17701 ataagtttta ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc taaatctaat
    17761 ttttcctgag tcatctcttc gccggtgatc gctttgaaga atttggcttc aagcgccaga
    17821 tcgccccggt aattacggct tttcagcggc gaaacggtca ttggccagac ccagttacac
    17881 agggtgacgg cgttatgcaa acagacccgc aacagcgacc acttagcata tttaattttt
    17941 gcgtcattga ttggcgtgta gtttttggtt tcatcgtagg catcttcaga accaaaaagc
    18001 tctttcgcca cttcacgttg cagtttcaat ggcaagccgg aaccaataaa gttgatatgg
    18061 gtatgcgtca tgcagtcacg gttgaacata cagttaacaa tggaaccgac ctgcgctgac
    18121 gcttcattgg catggtgaac cggatagcca aacggcgacc agagtttatt tttcgcgtag
    18181 ccccagtact cttcacccaa attccagcgt tctgcgatgg catatgaacc atcagccagg
    18241 tgactcagct cacccacacg atgcgccaga cggtagtaaa aatctttaat gaagttaacg
    18301 tcacccgctt ccagttgatc ccagcgaatt tccgcatact cttcagcagg cagaacacgc
    18361 ttgaacaccc ctttgctgta acaataggta aagtcgcgat gcaactgccc gtagttacac
    18421 cataggccgt agtcatcgaa caaattcaga cccaccaggt taccaatcac acggccatcg
    18481 tctttatctt caaaatcttt cggaccgttc gggaagatgg tggtatggac aaagttggct
    18541 acacaagtgt taccacctgt gctgggcacg ccaaactctt tcacccgagg aatattcatt
    18601 tgggtcatac aacggatcgg gcaagagtgg cagccactca tttttactgt gtatttctct
    18661 gccgccggac ctaagtcaaa aacggattta taggtacgaa agccgaccgt gttctgattg
    18721 cctggcggaa tttcccccgt ttcaatcgga ccaccttcag ccgcgcccca aaacagccct
    18781 ttacgtgctg tccaacgtga cttgggatct gaatactctg cccacgattg cggcgtactt
    18841 ggcacgacat ggttgttatt cgcaccaata agttcagtca tcatgtaatc gttgagacgc
    18901 ttcatctcct gacgatcggc aatgttgacc cctttcgtcc cttcaaccgc aatcgctttc
    18961 aggtttttcg aacccataat tgcgccagtt cccgcaccgc cgctgtggtt acggctattc
    19021 aacatgccag agagaggaac aaggttttcc ccagcctgac caatagccgc cacacaggtt
    19081 tccggactgg tcaatcgaca aatttcttcc gtcgttgcgc gcgtcccttt tccccataag
    19141 aaatcggctt tttccaggct aactttgtca tctttaatct tcagccatac cggtgatttc
    19201 gccttccctt cgataataat gacgtcgtat ccagcgaatt tcatttgcgc tgcaaaaaag
    19261 ccacccatat gggcatcgac aactaaattt cctttggtaa aagtagaaag tgaggtgata
    19321 tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc aaagactaat
    19381 ttattcgctt catcgaaagg tttcgtgcct ggcggtactt cgtcatacat aattttgtag
    19441 ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag ggtaatattt
    19501 cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat gattttttcc
    19561 tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc cctgttgggc
    19621 aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt tcggtattta
    19681 cggtggccat catccacggg catgccgtag tacaggcgct acagccaata cagcgtttat
    19741 gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc gggcagacgt
    19801 tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg agatcgccat
    19861 acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcgg tggattttga
    19921 tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag atttcacagc
    19981 ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt gtatttatca
    20041 accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac agtaacgcag
    20101 gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct aatcgcgtta
    20161 agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc tcttcgcaaa
    20221 cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc tgctgactct
    20281 gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat ttaacgccga
    20341 taaactaatc ctttatggtt aggagaaaaa ataatg
//
LOCUS       DAPPNJ010000071        20219 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000071 DAPPNJ010000000
VERSION     DAPPNJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.071"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(33..914)
                     /gene="prmA"
                     /locus_tag="RPM73_002151"
     CDS             complement(33..914)
                     /gene="prmA"
                     /locus_tag="RPM73_002151"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDX6520507.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(926..2377)
                     /gene="panF"
                     /locus_tag="RPM73_002152"
     CDS             complement(926..2377)
                     /gene="panF"
                     /locus_tag="RPM73_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDX6520508.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2367..2609)
                     /locus_tag="RPM73_002153"
     CDS             complement(2367..2609)
                     /locus_tag="RPM73_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDX6520509.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2718..4067)
                     /gene="accC"
                     /locus_tag="RPM73_002154"
     CDS             complement(2718..4067)
                     /gene="accC"
                     /locus_tag="RPM73_002154"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDX6520510.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4078..4548)
                     /gene="accB"
                     /locus_tag="RPM73_002155"
                     /gene_synonym="fabE"
     CDS             complement(4078..4548)
                     /gene="accB"
                     /locus_tag="RPM73_002155"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDX6520511.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5526..6500)
                     /locus_tag="RPM73_002156"
     CDS             complement(5526..6500)
                     /locus_tag="RPM73_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX6520512.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6652..8592
                     /gene="csrD"
                     /locus_tag="RPM73_002157"
     CDS             6652..8592
                     /gene="csrD"
                     /locus_tag="RPM73_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDX6520513.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8897..9940
                     /gene="mreB"
                     /locus_tag="RPM73_002158"
     CDS             8897..9940
                     /gene="mreB"
                     /locus_tag="RPM73_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDX6520514.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10006..11109
                     /gene="mreC"
                     /locus_tag="RPM73_002159"
     CDS             10006..11109
                     /gene="mreC"
                     /locus_tag="RPM73_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDX6520515.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11109..11597
                     /gene="mreD"
                     /locus_tag="RPM73_002160"
     CDS             11109..11597
                     /gene="mreD"
                     /locus_tag="RPM73_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDX6520516.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11606..12199
                     /gene="yhdE"
                     /locus_tag="RPM73_002161"
     CDS             11606..12199
                     /gene="yhdE"
                     /locus_tag="RPM73_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDX6520517.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12189..13658
                     /gene="rng"
                     /locus_tag="RPM73_002162"
     CDS             12189..13658
                     /gene="rng"
                     /locus_tag="RPM73_002162"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDX6520518.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13726..17526
                     /gene="yhdP"
                     /locus_tag="RPM73_002163"
     CDS             13726..17526
                     /gene="yhdP"
                     /locus_tag="RPM73_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDX6520519.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17682..19127
                     /gene="tldD"
                     /locus_tag="RPM73_002164"
     CDS             17682..19127
                     /gene="tldD"
                     /locus_tag="RPM73_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDX6520520.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19255..20184)
                     /gene="aaeR"
                     /locus_tag="RPM73_002165"
     CDS             complement(19255..20184)
                     /gene="aaeR"
                     /locus_tag="RPM73_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDX6520521.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
BASE COUNT         4811 a         5138 c         5508 g         4762 t
ORIGIN      
        1 tcgccccgcg tcaccctacg gcgatgcgaa ggttaattct tacgaccggt aatacggcac
       61 cactcttctt tttccacgac cgggtccagt gcgaagctgt cggcataagc ttcacaaacg
      121 ctctctgcct ggcttgccag aataccggaa aggcccagca aaccgcctga aaccggcagg
      181 acgctgatta acggtgccag ttcacgtaat gggcctgcaa ggatgttagc gaccaccacg
      241 tcggctttca tttcttctgg ctgatctttc ggtaagtaga gttccagacg gtcagaaacg
      301 ccattacgtt cggcgttgtc gcggctggcc tgaatcgcct gcggatcgat atcaatacca
      361 atggcttttg ctgcacccag tttcagcgcc gcgatcgcca gaatgccgga accacagcca
      421 aagtcgatga ctgttttacc ggttaaatcg aggctgtcga gccattgcag gcacagagag
      481 gtggttggat gggtacccgt accaaacgcc agccctggat ctaacatcac gttgacggcg
      541 ttttcgtccg gcacatcacg ccagctaggg cagatccaca gtcgttcacc aaagcgcatc
      601 gggtggaaat tatccatcca ttcgcgctcc cagtctttat cttctagttg ttcgatttta
      661 tgcgcgaagc ctgcgccgag cagcggatgg ttttccagaa tcgccaccac gtcgttcata
      721 tcggtttcag cgtcaaacag accaatcaca tcggtgtcgc cccacaggcg cgtttcgccc
      781 ggcagcggct caaatactgg cgtatcgtgg gtatcctgaa aagtgataga aacggcaccc
      841 gcttccatca gcgcatcgct aagatcttcc gcgttcgcgc cggtggtgtt cagtttcagt
      901 tggatccaag gcatggcaaa actctttatt tatcagtagt caaaacggta gcttgcggga
      961 cggatgtacc gaaacggttt ccgaccagga aagccagcaa acttagtagt aacgagggca
     1021 cgatagggtg gaagcccagg tactgaatat tcagcgtcgc gagtacggca tacagcacgc
     1081 cgccaacgat catcgcactt agcgcgcctt tggcgttggc gcgttcccag taaagaccca
     1141 gcaccagcgg ccacaggaaa acggcttcca gcccaccgaa ggccagcaaa ttcagccaga
     1201 tgatcatttc tggcggcttc caggcggcaa gcagcagcaa cgcgccgaga actaacgtaa
     1261 ttaccgccga catccgcttc agacgcgtct cgttttgcat ttgatccgga cggatattca
     1321 gatagagatc tttaatgatc gtagcggaac tttgcagcag ttgggcgtta attgtcgaca
     1381 tgatcgcagc cataggtgca gccagaaaga tcccggcagc aaaaggtggc agcactttta
     1441 ccattaacgt cgggatcacc agatccggta cggtgagatc ggggatcact gcacgaccta
     1501 acgctccggc caggtgcata ccgaacatca ggattgcgac tacaattgta ccgatgatga
     1561 tcccccggtg tactgctttg ctgtctttat aagagataca gcgcaccgca gtatgcggca
     1621 ggccaatcac gccaaagcac accagtaccc agaacgacgt cataaaggca ggcgacagaa
     1681 tatcgtcagc gccttgtggc gtaaccagtt gcggatcgat ggtttgcaag gtctgtactg
     1741 cgttgcttaa gccgccagcg gcatgtacca cgccaataag cagcacaacg gtgccaatca
     1801 gcatcacaag cccttgcatg gtgtcgttca gcacgctggc gcgaaagcca ccaaaggcgg
     1861 tatataacgc aatgctgata ccaaaaatca gcagcccggt ttcataagga atacccgccg
     1921 cggtttccag caggcgcgca ccgccgataa actgcacggt catcgcacca acgaacgcaa
     1981 ccagcaaact caaactcgcc agccacacca gaagacgact ctggtagcgg gcaaacagca
     2041 tgtcgttcag cgtcactgca ttgtagcggc gcgcaagaat cgcaaacttc ttgccgagaa
     2101 taccgagtga aagccagact gcaggaagct gaatcatcgc cagcaatacc cagcccagcc
     2161 cgtatttata agcagctcct ggcccgccga taaacgaact ggcactgata taggtcgcgg
     2221 tgagcgtcat cgccagcaca ataccgccca tagagcggct gccgaggaaa tactcattaa
     2281 ggaaggtgcc ggtgctccgt ttacgcatcg cataaaccga gataccgaac accaccacca
     2341 gataggcgac cagcggtaga attacttcaa gctgcatcgt catcctccag tgggatatcg
     2401 cgatagataa atttcaccat cgcccagcac agtccaataa acagtagcgg cgtcaggatg
     2461 caggccatct caaaccagcg cggaaagccg gtaaaaccgg gggcaacgcc aggtaagtaa
     2521 gcggctacta accaaactgc cagatacaaa agggtcagcc ccagcgccca gcgcgcctct
     2581 ttatgggcct gaacaaaacg agtgtccatt ttttgtccct gatgggtgaa gaaagcgagg
     2641 attgtacctt atgggggttg tcgatcccca gtaataaaaa aggccggaaa atccggcctt
     2701 ttgacgcttt tgcagtctta tttttcctga agaccgagtt ttttctccag atagtggatg
     2761 ttagtgccac catgctggaa gttctcgtca ttcatgatgc ggatctgcag atcaacgttg
     2821 gttttgatac cgtcgatgat cagctcctgc agcgcattct tcatgcgagc aatcgccacg
     2881 tcacggtttt caccgtagca aatcagctta ccgatcattg agtcatagta cggcggtacg
     2941 gtgtagcccg cgtagatatg agactcccaa cgtacgccaa aaccgccagg tgcgtggaaa
     3001 cgggtgattt tgcctgggct tggcaggaag gtgttcggat cttcggcgtt gatacgacat
     3061 tccaccgcat ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg ttgaccggca
     3121 gcgatacgca gctgttcttt gatcaggtca acgccggtga tcatttctgt aaccgggtgt
     3181 tctacctgaa tacgggtgtt catttcgatg aaatagaact cgccgttttc gaacaggaac
     3241 tcgaaagtac ctgcaccgcg atagccgata tcaacacacg ctttagcgca acgttcgccg
     3301 atgtagcgac gcagttccgg ggtaatgccc ggtgctggtg cttcttcgac cactttctgg
     3361 tggcggcgct gcatggagca gtcacgttcc gccagataga tagcgttgcc ctgaccgtca
     3421 gccagtacct gaatctcgac gtggcgagga ttttccaggt atttctccat gtaaaccata
     3481 tcgttgctga aagcagcttt cgcttccgca cgggtcatgg agatggattg tgccagttca
     3541 gcgtcgccgc gcactacgcg cataccgcga ccaccgccgc cgccggaggc tttgataatc
     3601 accggataac caatgcgttt agcaatggca cggtttttat ccatatcgtc gcccaacggg
     3661 ccgtcagaac ccggtacgca agggacgccc gcttttttca tcgccgcgat tgcggatact
     3721 ttgtcgccca tcaggcgaat ggtttctgct ttcgggccga tgaagataaa gccggagcgt
     3781 tcaacctgct cggcaaagtt ggcgttctcg gagaggaagc cgtaacccgg atggattgct
     3841 actgcgccgg tgatttcagc ggcgctgatg attgccggga tgttcagata actttttact
     3901 gacggagcag ggccaataca gaccgtttca tctgccagta atacgtgttt tagatcgcga
     3961 tccgcgctgg agtgcacagc gacagtcttg atgcccagtt ctttacaggc acgaagaata
     4021 cgcaatgcaa tctcgccgcg gttggcaata acaattttat ccagcatgtt cgcctcgtta
     4081 ctcgatgacg accagcggct cgtcaaattc taccggttgt ccactttcga ccagaattgc
     4141 tttcacggta ccggatttgt ccgcttcgat ctggttcatc attttcatgg cttcaacgat
     4201 gcacagggta tcgcccacgt tgactttctg acccacttcg atgaacgctt ttgcgtccgg
     4261 gcttggggtg cggtagaaag taccaaccat cggggaacgt acgatgtgac cactgatttc
     4321 cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt tagattgagc
     4381 tggctgctgc atcattggtg cagcgtaagc ttgttgcatc acagggaaac ttgcggcagg
     4441 agctgcacgg ctaatgcgta ctgactcttc gccttcagaa atttccagtt cggagatgcc
     4501 tgattcttca accagctcga tcagtttttt aatcttacga atatccatga gtgggttccg
     4561 tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa gcgtatgccc
     4621 cgacagctgt atgcatagcg ataaattcca gcaggccggg acgccggtct attttgcccc
     4681 agagtgtcaa tgttagactt gacggacatt gtgcagcgat ctgctgctat cctccggcaa
     4741 aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa aaactgcgtc
     4801 agggaggacg aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta aatactggtc
     4861 ttatcaggga agataatcaa cagctaacat gtaaataacc ttcaacaccg tgtaatttgc
     4921 aacaagccgc acaattcacg aaattagcgc catcatcgac ggaacttctt ataacgtaag
     4981 gtgaaaagct gtaaagccag ccctgcgaag atgaggggct gcaatgagat aatcttcaca
     5041 gaccacaaat aatgtaggga tgccaggatc gcgacaagat agacgacatt acgcagcggt
     5101 tgctggggat tacccaattt tcactgcatt gtctggatag atgttaatgc taaagcacgc
     5161 aaaattaacc gattgacaat gctcagcatt aaataggttc gagtgattaa tttgtagtca
     5221 aataaaatca gattattcac cccatggtac cagaaatcag taacaggtta agcgtagtgt
     5281 cgcccaggca aagcaccata aacctaacag gcgacggagg catatcaata atggctgttt
     5341 agaggagtat caatgcgcat caatttgtgg tcagtcgaaa tcatgacttt ccagtttgga
     5401 tgatgttgta caacttcctg accaaggtgt tcgacgaaag ctgtccggct aaccgctttt
     5461 cccgttaacc gattgaataa tgatgtcaaa atgagttccc tctcttatta ttccttgcta
     5521 aatggttagt taaccttcac cagcgtgcga ccctggatct ggttattaat gatggcctcg
     5581 gcaaagttcg gtgcctctga cagagatatc tcttttgccg cctgggtata gaatgattcc
     5641 ggtaaatcgg cgaccagtcg ctgccaggct tgtgcgcggc gttctggtgg cgtcattact
     5701 gaatccaccc cttgcaaacg gacattacgc agaataaatg gcatgaccgt ggttggcaga
     5761 gtaaaaccac ccgccagacc acaggcagcc acgcagccgc cgtaattcat ttgcgccagc
     5821 acttttgcca gcactttgtc gccaacagtg tcaattgccc cagcccagac ttgtttttcc
     5881 agaggacggg attcggcaaa ctcatcacga gggagaatac gactggcacc taaacttttc
     5941 agatattcat gggtactttc gcgaccggaa acggcaacga cctgataacc caacttatgc
     6001 agcagcgcca cggcggtact gccgacgcca ccactggcac ccgtcacgac aatctccccg
     6061 tcctgcgggc gaacaccggc atcttccagc gccatcacac acagcatggc ggtaaaaccg
     6121 gcagtaccga taatcattgc tttacgcgcg tccagccctt gcggcatggc aaccagccag
     6181 tcacctttca ctcgcgcctg ctccgccagc ccaccccagt ggttttcacc aacgccccag
     6241 ccagtgagta acacctcctg accggcatga aaacgcggat cttcgctggt gcgtacagtt
     6301 ccggcaaaat cgatcccagg aatcatcgga aaattacgga tgatttttcc cttaccggta
     6361 atcgccagcg catctttata gttcaggctc gaccagtgaa catcaaccgt gacatcgccc
     6421 tccggcaggc gactttcgtc aagtgtctgt accgatgcga gagttttgcc gtcctgctgt
     6481 tctaaaagta acgcctgcat aagtggtcct catgtgcatg atggattgga aaataatttc
     6541 tgaagactat actcgctaat ggaaaagcaa agccgatgaa gcgcaataaa ttgcgagata
     6601 aatctgattt gctagtatgc ccgcttcctc attatcggag ttaacacaag gatgagatta
     6661 acgacgaaat tttcggcctt tgttacgctg ctcaccgggt taacaatttt tgtgactttg
     6721 ctgggctgtt cgctaagttt ctacaacgcc attcagtata agtttagtca tcgcgttcag
     6781 gcggtggcga cggcgattga tacccacctt gtgtcgaatg acttcagcac attaaggcca
     6841 caaattaccg aattaatgat gtcggcagat atcgttcgtg tagacctgct ccatggtgat
     6901 aaacaggttt ataccctggc cagaaatggt agttatcgtc cggttggctc cagcgatctg
     6961 tttcgcgaac tgagtgttcc gttgataaag catccgggga tgtcgctgcg tctggtttat
     7021 caggatccga tgggcaacta tttccattct ttaatgacta ccgcgccgct cacgggggcg
     7081 attggcttta tcattgttat gctcttcctg gcggtacgct ggttacaacg gcaacttgcc
     7141 gggcaagaat tgctggaaac ccgggctact cgtatcttaa acggtgagcg tggctctaat
     7201 gtgttgggaa ccatctatga atggccgccc agaaccagca gtgcgctaga tacgctgctt
     7261 cgtgaaattc agaacgcacg cgaacaacac agccgtcttg atacgctgat ccgctcttat
     7321 gccgcccagg acgtgaaaac cggcctcaat aaccgactct ttttcgataa tcagttagca
     7381 acgttactgg aagatcagga gaaagtaggt acccacggga tcgtgatgat gattcgtctg
     7441 ccggatttca atatgttgag cgatacttgg gggcacagcc aggttgaaga acagttcttc
     7501 actctgacga atctgctgtc gacatttatg atgcgctacc ctggcgcact gctggcgcgt
     7561 taccaccgca gtgattttgc tgcgctgtta ccgcaccgga cgttaaaaga ggcagagagc
     7621 atcgccggtc agttaatcaa agccgtcgat accttgccga acaataaaat gctcgatcgc
     7681 gacgatatga tccacattgg tatctgcgcc tggcgtagtg gtcaggatac cgagcaggta
     7741 atggaacatg cagagtctgc cacgcgtaat gcgggattgc agggcggcaa tagctgggct
     7801 atttacgatg actcgttgcc tgaaaaagga cgcggtaatg ttcgctggcg tacgcttatc
     7861 gagcaaatgc tcagtcgcgg cggcccgcgc ctttatcaaa aaccggcggt tactcgcgaa
     7921 ggtcaggttc atcatcgcga actcatgtgc cgcatcttcg atggtaatga agaggttagc
     7981 tcggcggagt atatgccgat ggtcttgcag tttggcttat cggaagagta tgaccgtctg
     8041 caaatcagcc gtcttattcc actattgcgt tactggccag aggaaaatct ggcgattcag
     8101 gttaccgttg agtcgctgat tcgcccgcgt tttcagcgtt ggctgcgcga tacgttaatg
     8161 caatgtgaaa aatcacaacg aaaacgcata attattgaac ttgcagaggc cgatgtaggt
     8221 caacatatca gtcgtttaca acctgttatt cgtttagtga atgctttagg ggtacgggta
     8281 gccgtcaacc aggctggttt gacgctggta agcaccagtt ggatcaaaga acttaatgtt
     8341 gagttactca agctccatcc ggggctggtc agaaacattg agaagcgaac ggagaaccag
     8401 ctgctggttc aaagcctggt ggaagcctgc tccgggacca gcacccaagt ttacgccacc
     8461 ggtgtgcgtt cgcgaagcga gtggcagacc ctgattcagc gcggtgttac aggcgggcaa
     8521 ggggattttt tcgcgtcctc acagccactt gatactaacg tgaaaaaata ttcacaaaga
     8581 tactcggttt aacctgccgt ttaatccgtt ttcacgtaga ataacgcgcg ctgcgtctca
     8641 tgggagtgtg cttgtctgct cgccagattg ttgcagcaca tatgcagatg aatgacctta
     8701 cgcggttgca aacaggcgag gaatgctgct gatgcattaa gcctttctgg actcaggcag
     8761 agatttgtaa caaaggaaac gaactgcact aattttcacc gtagcagatg atttttgcgc
     8821 cttgtcgctg ctgcgtgtgg ttggtaaagt aagcggattt tcttttccgc cccagctttc
     8881 aggattatcc cttagtatgt tgaaaaaatt tcgtggcatg ttttccaatg acttgtccat
     8941 tgacctgggt actgcgaata ccctcattta tgtaaaagga caaggcatcg tattgaatga
     9001 gccttccgtg gtggccattc gtcaggatcg tgccggttca ccgaaaagcg tagctgcagt
     9061 aggtcatgac gcgaagcaga tgctgggccg tacgccgggc aatattgctg ccattcgccc
     9121 aatgaaagac ggcgttatcg ccgacttctt cgtgactgaa aaaatgctcc agcacttcat
     9181 caaacaagtg cacagcaaca gctttatgcg tccaagcccg cgcgttctgg tttgtgtacc
     9241 ggttggcgcg acccaggttg aacgccgcgc aattcgtgaa tccgcgcagg gcgctggtgc
     9301 ccgtgaagtc ttcctgattg aagaaccgat ggctgccgca attggtgctg gcctgccggt
     9361 ttctgaagcg accggttcta tggtggttga tatcggtggt ggtaccactg aagttgctgt
     9421 tatctccttg aacggcgtgg tttactcctc ttccgtgcgc attggtggtg atcgtttcga
     9481 cgaagctatc atcaactatg tgcgtcgtaa ttacggttct ctgatcggtg aagccaccgc
     9541 agaacgtatc aagcacgaaa tcggttcggc ttacccgggc gatgaagtcc gtgaaatcga
     9601 agttcgtggc cgtaacctgg cagaaggtgt tccacgcggt tttaccctga actccaacga
     9661 aatcctcgaa gcactgcagg aaccgctgac cggtattgtg agcgcggtaa tggttgcact
     9721 ggaacagtgc ccgccggaac tggcttccga catctccgag cgcggcatgg tgctcaccgg
     9781 tggtggcgca ctgctgcgta accttgaccg tttgttaatg gaagaaaccg gcattccagt
     9841 cgttgttgct gaagacccgc tgacctgtgt ggcgcgcggt ggcggcaaag cgctggaaat
     9901 gatcgacatg cacggcggcg acctgttcag cgaagagtaa tcggatgcag gcaggggaaa
     9961 tgtctgttta ccctgcctgg tctgatacga gaatacgcat aacttatgaa gccaattttt
    10021 agccgtggcc cgtcgctaca gattcgcctt attctggcgg tgctggtggc gctcggcatt
    10081 attattgccg acagccgcct ggggacgttc agtcaaatcc gtacttatat ggataccgcc
    10141 gtcagtcctt tctactttgt ttccaatgct cctcgtgaat tgctggatgg cgtatcgcag
    10201 acgctggcct cgcgtgacca actagaactt gaaaaccggg cgttacgtca ggaactgttg
    10261 ctgaaaaaca gtgaactgct gatgcttgga caatacaaac aggagaacgc gcgtctgcgc
    10321 gagctgctgg gttccccgct gcgtcaggat gagcagaaaa tggtgactca ggttatctcc
    10381 acggttaacg atccttatag cgatcaagtt gttatcgata aaggtagcgt taatggcgtt
    10441 tatgaaggcc agccggtcat cagcgacaaa ggtgttgttg gtcaggtggt ggccgtcgct
    10501 aaactgacca gtcgcgtgct gctgatttgt gatgcgaccc acgcgctgcc aatccaggtg
    10561 ctgcgcaacg atatccgcgt aattgcagcc ggtaacggtt gtacggatga tttgcagctt
    10621 gagcatctac cagcgaatac ggatattcgt gttggtgatg tgctggtgac ttccggtctg
    10681 ggcggtcgtt tcccggaagg ctatccggtc gcggttgtct cttccgtaaa actcgatacc
    10741 cagcgcgctt atactgtgat tcaggcgcgt ccgacagcag ggctgcaacg tttgcgttat
    10801 ctgctgctgc tgtggggggc agatcgtaac ggcgctaacc cgatgacgcc ggaagaggtg
    10861 catcgtgttg ctaatgaacg tctgatgcag atgatgccgc aggtattgcc ttcgccagac
    10921 gcgatggggc caaagttacc tgaaccggca acggggatcg ctcagccgac tccgcagcaa
    10981 ccgacgacag gaaatgcagc tactgcgcct gctgcgccga cacagcctgc tgctaaccgc
    11041 tctccacaaa gggctacgcc gccgcaaagt ggtgctcaac cgcctgcgcg tgcgccggga
    11101 gggcaatagt ggcgagctat cgtagccagg gacgttgggt aatctggctc tctttcctca
    11161 ttgcgctgtt gctgcaaatc atgccctggc cggataacct gattgttttc cggccaaact
    11221 gggtgttact catcttgttg tattggatcc tggccttgcc tcatcgcgta aatgtgggca
    11281 caggttttgt gatgggtgcc atactggatc tgatcagcgg ctcgacgctt ggcgtacgcg
    11341 tattggcgat gagcatcatt gcttacctgg tggcgctgaa ataccagctt ttccgcaacc
    11401 tcgcattatg gcagcaggcg ctggtcgtca tgttgctttc gctggtggtg gatattattg
    11461 ttttctgggc agagttttta gtgattaacg tctctttcag accggaagtg ttctggagta
    11521 gtgtagtcaa tggggtgctc tggccgtgga ttttcttgct gatgcgtaaa gtccgtcagc
    11581 agtttgcagt gcaataaagg tttctatgac ttctctgtat ttagcttccg gttctccgcg
    11641 tcgtcaggag ttacttgcgc aacttggcgt gacctttgaa cgtattgtta caggcattga
    11701 ggagcagcgt cagccgcagg agagcgcgca gcagtatgtt gtgcgtctgg cgcgcgagaa
    11761 agcacaggcg ggtgtcgcgc aaacggcgca ggatctcccg gtgctgggtg cggatactat
    11821 cgttatcctg aatggagaag tgctggagaa accgcgcgac gcagagcatg cggcgcagat
    11881 gttgcgcaaa ttatcgggtc agacccatca ggtgatgacg gcagtggcgt tggccgacag
    11941 ccagcacatt ctcgattgcc tggtggtcac cgatgtgact ttcagaacgt taacagacga
    12001 agacatcgcg ggctatgtcg ccagcggtga accgttagat aaagcaggtg catacggtat
    12061 tcaggggctg ggtggctgtt ttgtcaggaa gataaatggc agctatcacg ccgtagtcgg
    12121 cttaccgctg gttgaaacgt atgaattatt aagtaatttt aacgcactgc gtgagaaaag
    12181 ggataaacat gacggctgaa ttgttagtaa acgtaacgcc ttcggaaacg cgagtggcgt
    12241 atattgatgg cggtattctg caggaaattc atattgaacg tgaggcgcga cgcggaatag
    12301 taggcaatat ctacaagggt cgtgtaagtc gtgtacttcc gggtatgcag gcggcttttg
    12361 tagatattgg gctggataaa gccgcgtttc ttcatgcatc cgatatcatg ccgcacaccg
    12421 aatgtgtggc gggtgaagaa caaaagcaat tcacggtgcg cgacatctcg gaactggttc
    12481 gtcaggggca agatctgatg gtgcaggtgg tgaaagatcc gcttggcact aaaggtgcgc
    12541 gcctgaccac cgatatcacg ctgccttctc gctatctggt ctttatgcca ggggcttctc
    12601 acgttggtgt ttcccaacgt attgaaagcg aatcagaacg tgaacgcctg aaaaaagtgg
    12661 tcgcagagta ttgcgacgag cagggcgggt ttatcatccg taccgcagcg gaaggggttg
    12721 gcgaggctga actggcctcc gatgccgctt atctgaaacg cgtctggacc aaagttatgg
    12781 agcgtaaaaa acgcccgcag acccgttatc agctgtacgg cgaactggcg ctggcgcagc
    12841 gtgttctgcg tgatttcgcc gacgccgaac tggaccgcat tcgcgttgac tcacgcctga
    12901 cttacgaagc attgctggag tttacctcgg agtacattcc cgagatgacc agcaagctgg
    12961 agcattacac cggacgccag ccgattttcg atctctttga tgtcgaaaac gaaatccagc
    13021 gagcgctgga acgcaaagta gaactgaaat ccggtggtta tctcattatc gaccagaccg
    13081 aagcgatgac caccgtggac atcaataccg gagcgtttgt cggtcatcgc aatctggacg
    13141 acaccatttt caataccaat attgaagcga cgcaggctat cgctcgccag ttacggttgc
    13201 gtaatctggg tgggattatc attattgatt tcatcgatat gaataatgaa gatcaccgcc
    13261 gccgcgtgct gcactcgctg gagcaggcgt tgagcaaaga ccgggtgaaa accagcgtta
    13321 atggtttttc ggcgctgggg ctggtggaga tgacgcgtaa acgcacccgc gaaagcattg
    13381 agcacgtact gtgtaacgaa tgcccaacct gccatggtcg cggaacggtg aaaaccgtgg
    13441 aaacggtatg ctatgaaatc atgcgcgaga ttgttcgtgt ccaccatgct tacgactccg
    13501 accgtttcct ggtctatgct tctccggcag tagctgaagc cttgaaaggc gaagagtcac
    13561 actcgctggc ggaagtggaa attttcgttg gcaaacaggt taaagtacaa attgaaccgc
    13621 tctataacca ggagcagttt gacgtcgtaa tgatgtaaac agatgctggg ccgccatccg
    13681 gcaaagggtt tttgagtcac atttttagca gacaaggagt gacgggtgag gcgattgccg
    13741 gggattttac tgcttactgg agccgcgctc gttgtgatcg ctgccctgct ggttagcggc
    13801 ctgcgtattg ctttaccgca tcttgacgcc tggcgtccgg aaatcctcaa caaaatagaa
    13861 tccgcgactg gcatgccggt agaagccagt cagctctcag ccagctggca gaattttggc
    13921 ccgacgcttg aagcacacga catccgtgca gaactaaaag atggcggcga attttcggtt
    13981 aaacgcgtta ctctggcgct ggatgtctgg cagagcttgt tacatatgcg ctggcagttt
    14041 cgcgacctca ctttctggca gctgcgcttt cgcaccaaca ctcctatcac cagcggtggt
    14101 ggtaatgata gcctggaagc cagtcacatc agcgatctgt ttcttcgtca atttgaccat
    14161 ttcgatctcc gcgacagtga agtcagtttt ctgacgccat ccggtcagcg cgccgagctg
    14221 gcgatcccac aactcacctg gctgaacgat ccacgtcgac accgtgcgga aggcctggta
    14281 agcctctcca gccttaccgg acagcacggc gtgatgcagg tgcgcatgga tttgcgcgat
    14341 gatgaggggt tgttaagcaa tggtcgcgtc tggctacagg cggatgacat cgacctgaag
    14401 ccgtggctcg gtaaatggat gcaggacaat attgctctgg aaacggcaca gttctccctt
    14461 gaaggctgga tgacgatcga caaaggcgat gtaaccggcg gtgacgtctg gctgaaacag
    14521 ggcggtgcca gctggttggg cgagaagcaa acgcatacgc tgtcggtgga taatctgacc
    14581 gcgcatatta cgcgtgaaaa tccgggctgg cagttctcta ttcccgatac acggatcacg
    14641 atggacggca aaccctggcc gagcggagca ttgacgctgg cctggatacc ggaacaggac
    14701 gttggcggca aagacaataa acgcagtgac gaactccgga ttcgcgccag taatctggag
    14761 ctggcaggcc tggagggcat acgcccgctg gccgcgaaac tttcacctgc actgggtgat
    14821 gtttggcgct ccacacaacc gagcggcaag attaacactc tggcgctgga tatcccgctt
    14881 caggcggcag acaagacccg ttttcaggca tcgtggagcg atctggcctg gaagcaatgg
    14941 aaattattac cgggtgcgga acacttctcc gggacgcttt ccggcagcgt tgaaaatggt
    15001 ttgcttaccg cgtcgatgaa gcaggcaaag atgccttacg aaacggtatt ccgtgcgcca
    15061 ctggaaatcg ccgacggcca ggcaactata agctggctga acaatgacaa aggtttccag
    15121 ctggatgggc gtaatattga cgttaaagcc aaagccgtcc atgcgcgcgg cggttttcgt
    15181 tacctgcaac ctgctaacga tgaaccctgg ctgggtattc tggctggcat cagtaccgat
    15241 gatggttcac aagcctggcg ctatttcccg gaaaacttga tgggtaaaga cctggttgat
    15301 tacttaagtg gcgcgattca gggcggtgaa gcggataacg cgacgctggt ttatggtggc
    15361 aatccgcaac tcttccccta taaacacaac gaaggtcagt ttgaagtgct ggtgccgctg
    15421 cgcaacgcga agtttgcctt ccagccggac tggcctgcat taactaacct tgatattgaa
    15481 ctggacttta ttaacgacgg tttatggatg aaaaccgatg gcgttaatct gggcggcgtg
    15541 cgcgcgagta atctcaccgc agtgatccct gactactcga aagaaaaact gctgattgac
    15601 gctgacatta aaggtccggg taaagccgtt ggcccttact ttgatgagac accgctgaaa
    15661 gattctctgg gtgcgaccct gcaagaactc cagctcgacg gcgatgtgaa tgctcgctta
    15721 catcttgata tcccgctgaa cggcgaactg gtaaccgcga aaggtgaagt gacgctgcgt
    15781 aataacagtc tgtttatcaa accactcgac agcaccctga aaaatttgag cggtaaattc
    15841 agctttatca atggcgatct gcaaagtgaa ccactgacag caagctggtt taatcagccg
    15901 ttgaacgtgg atttttccac caaagaaggg gcaaaagcct accaggttgc ggtgaatctc
    15961 aacggtaact ggcaaccggc gaaaaccagc gttctgcctg aagcggtgaa cgaagcattg
    16021 agtggcagcg tggcgtggga tggtaaagtg ggcattgatc tgccttatca tgctggcgcg
    16081 acctataacg tagagatgaa cggcgatctg aataatgtga gtagtcactt accttcaccg
    16141 ttagccaaac ctgcgggtga accactagcg gtaaacgtta aggttgatgg caatctcaac
    16201 agctttgaat taaccggaca ggctggtgcg gataaccatt tcaatagccg ctggttgctt
    16261 ggtcaaaagc tgacgctcga tcgtgctatt tgggcggcag acagtaaaac gctcccgccg
    16321 ttgccggaac aaagtggcgt tgaactcaat atgccgccga tgaatggtgc cgagtggctg
    16381 gccctgttcc agaaaggcgc tgcggagagt gtcggtggtg cagcgagttt cccacaacac
    16441 ataacgttac gtacgcctat gttgtcactg ggaaatcagc aatggaataa cctgagtatt
    16501 gtttcgcaac cgacggcaaa tggcaccctg gttgaggcgc aagggcgtga aatcaacgcc
    16561 acgctagcga tgcgtaataa cgcgccgtgg ctggcgaata tcaaatatct ttattacaac
    16621 ccgagcgtgg cgaaaactcg tggtgattca acgccgtcat cacctttccc gacaacggag
    16681 cgcattaact tccgtggctg gccggacgca caaatacgat gcacagagtg ctggttctgg
    16741 gggcaaaaat tcggtcgcat tgacagtgat atcaccattt ctggcgatac gttaacgctg
    16801 accaatggac tgattgatac tggtttctcg cggcttactg ccgatggtga atgggttaat
    16861 aatccggaga atgaacgtac ctcgctgaaa ggaaaactgc gcgggcagaa aattgatgcc
    16921 gccgcagaat tttttggtgt cacgacgccc atacgccagt cgtcatttaa tgtggattac
    16981 gatttacact ggcgcaaagc accctggcaa ccagatgagg cgacgttgaa tggcatcatt
    17041 catactcaac tgggtaaagg cgaaattacc gaaatcaata ccggacatgc cgggcaattg
    17101 ctgcgcttat tgagcgttga tgccctgatg cgtaagctgc gttttgattt cagagacact
    17161 tttggcgaag ggttctattt tgactccatt cgcagcaccg cgtggattaa agacggggtt
    17221 atgcacaccg acgacacgct ggtggatggc ctggaggcgg atatcgccat gaaagggtcg
    17281 gtaaatctgg tacgtcgcga cctgaatatg gaagcggttg tcgcaccaga gatttctgcg
    17341 acggtgggcg tggctgcggc ttttgcggtt aaccccattg ttggcgcggc agtatttgca
    17401 gccagtaaag tgctggggcc gctgtggagc aaagtctcca ttttgcgcta tcacatttcg
    17461 ggtccgctgg acgatccgca aatcaacgaa gtgttgcgcc aaccgcgtaa agaaaaagcg
    17521 caatgatttg acgagggcgc gtaattgccc caatctcata tgataatcgt tgccaaaggc
    17581 caacgagccg gaacataact gtaggccgga tagggcgttc acgccgcatc cggcagccgt
    17641 aaaaaatcct ctactgcagt aactaacgag tagcaaaaac gatgagtctg aacctggtaa
    17701 gtgaacaatt gctagcggcg aacggcctga aacatcagga cttgttcgcg atcctcggtc
    17761 aactggccga acgtcgcctt gattatggcg atctctattt tcagtcgagc tatcacgaat
    17821 cctgggtttt agaagaccgc attattaaag atggttctta caacatcgat cagggcgttg
    17881 gtgtgcgtgc aatcagtggt gaaaaaaccg gatttgctta cgctgaccaa atcagcctgc
    17941 tggcgctgga acagagtgcg caagcggcgc gcaccatcgt ccgtgatagt ggtgatggca
    18001 aagtacagac gctgggcgcg gtagagcata gcccgttgta tacctcggta gatccgctgc
    18061 aaagcatgag ccgtgaagag aagctggata tcctgcgtcg cgtcgataag gttgcccgcg
    18121 aagcggacaa gcgcgtacaa gaagtgactg ccagcctcag cggcgtttat gaattaattc
    18181 tggttgcggc caccgacggc acgctggcgg cggatgttcg tccgctggtg cgtctttccg
    18241 tgagcgttct ggtcgaagaa gatggcaaac gcgaacgcgg tgccagtggc ggcggcggtc
    18301 gttttggtta tgagttcttc cttgccgatc ttgacggcga agtccgtgcg gatgcatggg
    18361 caaaagaagc agtgcgtatg gcgctggtca atctttctgc cgttgctgca ccagcgggca
    18421 ccatgccggt agtacttggc gcaggttggc cgggcgtgct gttgcatgaa gcggtaggtc
    18481 acggtctgga aggcgacttc aaccgccgcg gcacttcagt atttagtggg caggtcgggg
    18541 agctggtggc ttcagaactg tgtaccgtgg ttgatgacgg cacgatggtc gatcgccgtg
    18601 gttcggtggc gattgatgac gaaggtacgc caggccagta caacgtgctg attgagaacg
    18661 gcattctgaa aggctacatg caggataaac tcaacgcgcg tttgatgggg atgacgccga
    18721 ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat gccgcgtatg accaacacct
    18781 atatgctgcc gggtaaatcg accccgcagg aaattattga atccgttgag tacggtatct
    18841 atgcaccgaa ctttggtggc ggtcaggtgg atattacatc cggcaaattc gttttctcca
    18901 cttcagaagc gtatctgatt gaaaacggta aagtaacgaa gccggtgaaa ggcgcgacgt
    18961 tgattggttc cggtatcgaa accatgcagc agatttcgat ggttggcaac gacctgaaac
    19021 tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag tttgccggtt ggcgtgggcc
    19081 agccaacgct gaaagttgat aacctgactg ttggcggtac tgcgtaataa tttcattatt
    19141 ttcagaagga taattaatct ttctacgtgc acgaacggtc ccctcgctcc tctggggtta
    19201 gggtgagggg aacccgttgg cacaggtttg aacaacgtaa cagtacaata tgaattactt
    19261 ctctttcccg cgcccatgca tctcctgaaa caatttaccg acctcaacaa aataatccgt
    19321 cagcgagttg atcacgacct gtaccttcag cggcagttta tctttttcgg tatacaacgc
    19381 ataaaccggg cgtggatctg actggtaacg cggcagcagg atctccagct ccccacgatt
    19441 gatctcgttg atcacccaca tcagcggcac gtaggcgatc ccggcacccg ccgtcagcca
    19501 gcgcaccagc gtcatcggat cattagtcac aaatcttcct tgtgggatca ggcgagtcga
    19561 gatcccttcc ggtgcgatca gttcaaattc attgtcgggc cgcacgctgt attcaagcca
    19621 tgaatgacta ctcaaatcgg cgggtttttc cggtatgccg tattgtgtga gatagctttt
    19681 cgcggcgcac accaccatcg gcatcgcgcc cagacggcgg gaaaacaggc tggaatcctg
    19741 caacgcgccg acgcggatta ccacatccag accatcggca atcaggtcgg gggcaggaat
    19801 tccggtaacc agattgacgc tcaaacctgg gtattctttc agcatttttg ctgtcagccc
    19861 ggcgagaaca ttttgtgcca tagttgaaga acagccaatg cgcagcgtcc cgatgggggt
    19921 gttattgaag gcatacagtt gctcatgaac atcctgcact tcatggagca tccgacggca
    19981 gccctggtag taaattctgc cggcttcagt cagaccaatg ctgcgtgtac tacggtttaa
    20041 cagctttacc tgcaactcat cttccagttt tgacaccgtc tgactgatgg acgaaacgct
    20101 catctgtagc tgtctggcgg cggcggtaaa agagccaaat tcaactactt tggcaaacac
    20161 cgacatgcgt tttagtcgtt ccattattca ctctgactta aaagtgattt agatcacat
//
LOCUS       DAPPNJ010000072        20145 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000072 DAPPNJ010000000
VERSION     DAPPNJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.072"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1397
                     /locus_tag="RPM73_002166"
     CDS             <1..1397
                     /locus_tag="RPM73_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDX6520522.1"
                     /translation="TQSQRTWKTFNYFTLWMGSVHNVPNYVMVGGFFILGLSTFSIML
                     AIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVRGALFPGLLRGGIAAIMWFGL
                     QCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLITFLLFWLVNVGIGFGGGKVL
                     NKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSGIQKAENSGFLFLVVINAVVA
                     VWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWN
                     VLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIA
                     SIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMMAHYFVVMRGQINLDELYTAP
                     GDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLSRVSWFVGVIVAFAAYALLKK
                     RTTVEKTGEQKTIG"
     gene            1457..2818
                     /gene="allB"
                     /locus_tag="RPM73_002167"
     CDS             1457..2818
                     /gene="allB"
                     /locus_tag="RPM73_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDX6520523.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDSLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMDAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2875..4176
                     /locus_tag="RPM73_002168"
     CDS             2875..4176
                     /locus_tag="RPM73_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDX6520524.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4198..5343
                     /gene="glxK"
                     /locus_tag="RPM73_002169"
     CDS             4198..5343
                     /gene="glxK"
                     /locus_tag="RPM73_002169"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDX6520525.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5472..6257)
                     /gene="allE"
                     /locus_tag="RPM73_002170"
     CDS             complement(5472..6257)
                     /gene="allE"
                     /locus_tag="RPM73_002170"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDX6520526.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6268..7503)
                     /gene="allC"
                     /locus_tag="RPM73_002171"
     CDS             complement(6268..7503)
                     /gene="allC"
                     /locus_tag="RPM73_002171"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDX6520527.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7525..8574)
                     /gene="allD"
                     /locus_tag="RPM73_002172"
     CDS             complement(7525..8574)
                     /gene="allD"
                     /locus_tag="RPM73_002172"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDX6520528.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8891..10558
                     /locus_tag="RPM73_002173"
     CDS             8891..10558
                     /locus_tag="RPM73_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDX6520529.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLM
                     ENVAVINIGLRSFALELQSASKSVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10568..11827
                     /locus_tag="RPM73_002174"
     CDS             10568..11827
                     /locus_tag="RPM73_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDX6520530.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNPGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11838..12653
                     /locus_tag="RPM73_002175"
     CDS             11838..12653
                     /locus_tag="RPM73_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDX6520531.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12650..13543
                     /locus_tag="RPM73_002176"
     CDS             12650..13543
                     /locus_tag="RPM73_002176"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDX6520532.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13738..14805)
                     /gene="purK"
                     /locus_tag="RPM73_002177"
     CDS             complement(13738..14805)
                     /gene="purK"
                     /locus_tag="RPM73_002177"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDX6520533.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(14802..15311)
                     /gene="purE"
                     /locus_tag="RPM73_002178"
     CDS             complement(14802..15311)
                     /gene="purE"
                     /locus_tag="RPM73_002178"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDX6520534.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15429..16151)
                     /gene="lpxH"
                     /locus_tag="RPM73_002179"
     CDS             complement(15429..16151)
                     /gene="lpxH"
                     /locus_tag="RPM73_002179"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDX6520535.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16154..16648)
                     /gene="ppiB"
                     /locus_tag="RPM73_002180"
     CDS             complement(16154..16648)
                     /gene="ppiB"
                     /locus_tag="RPM73_002180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDX6520536.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16822..18207
                     /gene="cysS"
                     /locus_tag="RPM73_002181"
     CDS             16822..18207
                     /gene="cysS"
                     /locus_tag="RPM73_002181"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDX6520537.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18243..18764)
                     /locus_tag="RPM73_002182"
     CDS             complement(18243..18764)
                     /locus_tag="RPM73_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX6520538.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18872..19084)
                     /gene="ybcJ"
                     /locus_tag="RPM73_002183"
     CDS             complement(18872..19084)
                     /gene="ybcJ"
                     /locus_tag="RPM73_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDX6520539.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19086..19952)
                     /gene="folD"
                     /locus_tag="RPM73_002184"
     CDS             complement(19086..19952)
                     /gene="folD"
                     /locus_tag="RPM73_002184"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDX6520540.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         4582 a         5130 c         5547 g         4886 t
ORIGIN      
        1 aaacgcaaag ccagcggacc tggaaaacat ttaactattt taccttatgg atgggttcgg
       61 ttcataacgt tcccaattat gtgatggtcg gcggcttttt tattctcggc ttgtctacct
      121 ttagtattat gctggcaatt atcctcagcg cctttttcat tgccgcggta atggtattaa
      181 acggtgctgc gggcagtaaa tacggcgtac cgtttgccat gatcctgcgt gcttcttacg
      241 gcgtacgtgg cgcactgttt cccggattat taagaggtgg tattgctgcc attatgtggt
      301 ttggcctgca atgttacgcc ggatcactgg cctgcttgat cctgattggc aaaatctggc
      361 cgggattttt aactctcggt ggtgatttta ccttgttagg cctttcgcta ccgggcttaa
      421 ttactttctt actcttctgg ctggttaacg ttggtattgg ttttggcggt ggcaaagttt
      481 taaataaatt cactgccatt cttaacccgt gcatctatat cgttttcggc ggcatggcga
      541 tttgggcgat ttcactggtc gggatcggtc caatctttga ctacattccg agcggtattc
      601 agaaagcaga aaacagtgga ttcttgttcc tggtggtgat taacgcggta gttgcggtct
      661 gggcggcacc ggcggtgagc gcatccgact ttacgcaaaa cgcccactcg tttcgtgagc
      721 aagcgctggg gcaaacgctg ggtttagttg tggcctatat tctgtttgcg gtcgctgggg
      781 tatgtattat tgccggagcc agtattcact acggcgctga cacctggaac gtgctggata
      841 ttgttcagcg ttgggacagc ctgtttgcct cgttctttgc ggtactggtt attctgatga
      901 cgactatttc cactaacgcc accggtaata ttattccggc cggttatcag attgctgcca
      961 ttgcaccgac aaaactgacc tataaaaacg gcgtactgat tgccagtatt atcagcctgc
     1021 tgatctgccc gtggaaatta atggaaaatc aggacagtat ttatctgttc ctcgatatca
     1081 tcggcggaat gctgggtccg gtaattggtg tcatgatggc acattatttt gtggtgatgc
     1141 gtgggcaaat taatcttgat gaactgtaca ccgcacctgg cgattataaa tattacgata
     1201 acggttttaa cctcaccgcg ttttcagtaa ctctggtggc cgttatttta tctcttggcg
     1261 gtaagtttat tccctttatg gaaccgttat cgcgtgtttc atggtttgtc ggcgtcatcg
     1321 tcgcctttgc ggcctacgcc ttattaaaga aacgtacaac agtagaaaaa acaggagagc
     1381 aaaaaaccat aggttaatta atcccgatat tgaacattga gttaaaaacc aatctgtatt
     1441 ttacaaggag tttgttatgt cttttgattt aatcattaaa aacggcaccg ttattttaga
     1501 aaacgaagct cgcgttgtag atatcgccgt taaaggcggg aaaattgctg ctatcggtca
     1561 ggatctgggc gatgcaaaag aagttatgga tgcgtctggt ctggtggttt cgccgggcat
     1621 ggttgatgcg cacacccata tttctgaacc gggtcgtagc cactgggaag gttatgaaac
     1681 cggtactcgc gcagcggcaa aaggtggtat caccaccatg atcgaaatgc cgctcaacca
     1741 gctgcctgca acggttgacc gcgcttcaat tgaactgaag ttcgatgccg ctaaaggcaa
     1801 gctgactatc gatgcggcac aactcggtgg cctggtgtct tacaacatcg acagtctgca
     1861 tgagctggat gaagtgggcg ttgtcggctt caaatgcttc gttgcgacct gtggcgatcg
     1921 cggtatcgac aacgacttcc gtgatgtaaa cgactggcag ttcttcaaag gtgcgcagaa
     1981 gctgggcgaa ctggggcagc cggtgctggt gcactgcgaa aacgcgctga tctgtgacgc
     2041 actgggcgaa gaagcgaaac gtgaaggtcg cgtaactgcc catgactatg tggcttcgcg
     2101 tccggtattt accgaagtgg aagcgattcg ccgcgtgctg tatctggcga aagttgccgg
     2161 ttgccgtctg cacgtttgcc atatcagcag cccggaaggc gttgaagaag tgactcgtgc
     2221 acgtcaggaa ggtcaggacg ttacttgtga atcctgcccg cattactttg tactggatac
     2281 cgatcagttc gaagaaatcg gcactctggc gaagtgttca ccgccgatcc gcgatctgga
     2341 aaaccagaaa ggcatgtggg aaaaactgtt taacggtgaa atcgactgcc tggtttccga
     2401 ccactcacca tgccctccgg aaatgaaagc cggcaacatc atggatgcat ggggcggtat
     2461 tgccggtctg caaaactgta tggacgtgat gttcgatgaa gcggtacaga aacgcggaat
     2521 gtctctgcca atgttcggca aattaatggc gactaacgca gcagatattt tcggtctgca
     2581 gcaaaaaggc cgtatcgccc caggaaaaga tgccgacttc gtcttcattc agccgaatag
     2641 cagctatgtt cttaccaatg acgatctgga atatcgccac aaagtcagcc cgtatgttgg
     2701 ccgtaccatt ggcgcgcgta tcacgaaaac catcttacgt ggtgatgtga tttacgacat
     2761 tgaacagggc ttccctgttg cgccgaaagg tcaatttatc cttaaacatc agcagtaatc
     2821 tggcccctgc aatgcccgtc cttgcggcgg gcattctccg gttaaggtgt gtttatgttc
     2881 aattttgcag tcagccgcga aagcctgtta tcaggatttc agtggttttt ctttattttt
     2941 tgcaacacgg ttgtggttcc tcctacgcta ctttctgctt ttcagttgcc gcaaagtagc
     3001 ctgcttacgc tcacgcaata tgcttttctt gctaccgcac tggcctgctt cgctcaggcg
     3061 ttctgcggtc atcgtcgcgc tattatggaa gggccgggtg gcctgtggtg gggaaccatc
     3121 cttactatca cccttggcga agcatcgcgc gggacaccga tcaacgatat cgctaccagc
     3181 ctggcggtgg ggattgcgct ctccggcgtg ctgacgatgt tgattggttt tagcggatta
     3241 ggccatcgcc tggcacggtt atttacgccg tcggtgatgg tcttgtttat gttgatgctg
     3301 ggcgcgcagc tgaccactat ctttttcaaa ggtatgctcg ggctgccgtt tggcatagcc
     3361 gacccaaatt ttaaaattca gttaccgccg ttcgcgctct cggtggcggt gatgtgcctg
     3421 gtactgggga tgattatctt cctgccgcaa cgttttgccc gttatggcct gctggtcggc
     3481 accataaccg gctggttgtt gtggtacttt tgctttcctt cttcgcactc gctctccggt
     3541 gagttgcact ggcagtggtt cccgctcggc agtggaggtg ctttgtctcc gggaattatt
     3601 ctgacggcgg tgattacagg tctggtgaat atcagcaata cctacggtgc gattcggggc
     3661 acggatgttt tttatccgca gcagggcgcg gggaatacgc gttatcgtcg cagctttgtg
     3721 gcgaccggat ttatgacgct gataaccgta ccgctggcgg taattccatt ttcaccgttt
     3781 gtttcatcca ttggtttatt aacccagaca ggcgattaca cgcggcgttc gtttatttat
     3841 ggcagcgtta tttgcctgct ggtggcgctg gttccggcac tcacacgact gttttgcagt
     3901 atccctttac ccgtgagtag cgcggtcatg ctggtttctt atctgccttt actcttttcc
     3961 gcgctggtgt ttagccagca aataaccttt accgctcgca atatttatcg actcgcattg
     4021 ccgttatttg tcggcatatt tttaatggca ttaccgcctg tgtatctgca agaccttcca
     4081 ttaacgctgc gtcctctgct cagtaacggc ttactggtcg gaattttact ggctgttctt
     4141 atggataatc ttattccgtg ggaacgcatc gaataatttg ttgaaaaagg attgataatg
     4201 aagattgtca ttgcgccaga ctcttttaaa gagagcttaa gtgcagaaaa atgttgtcag
     4261 gcaattaaag ccgggttttc gaccctcttt cccgatgcga actatatctg tttgccgata
     4321 gcggatggcg gcgaagggac ggtggatgcg atggtcgccg cgacgggcgg caacatcgtg
     4381 acgcttgaag tctgcgggcc gatgggcgaa aaagtgaatg ctttttatgg ccttaccggc
     4441 gacgggaaaa cggcggtgat tgagatggcg gcagcaagtg gcctgatgct ggtcgcgcct
     4501 gaaaagcgta atccgttgct ggcctccagt tttggtacgg gggagttaat tcgtcatgcg
     4561 ctggataacg gcattcgcca tattattctc ggcattggcg gcagtgcgac ggtcgacggt
     4621 ggtatgggca tggcgcaggc gctcggtgtg cgtttccttg atgccgacgg tcaggtgctg
     4681 gtggctaacg gcggtaattt agcgcgcgtg gcaagcattg agatggatga atgcgatccg
     4741 cgtctggcga attgccatat tgaagtagca tgtgacgttg ataacccgct ggtaggggca
     4801 cgcggcgcgg cggcggtgtt tggcccgcaa aaaggggcaa cgccggaaat ggtcgaagag
     4861 ctggaacagg ggctgcaaaa ttacgcccgt gttttacaac agcaaactga aatcaacgtc
     4921 tgccagatgg cgggcggcgg cgctgcgggc ggtatgggta ttgcggcggc ggtatttctc
     4981 aatgcggata ttaaaccagg tattgagatt gtgttgaatg cggtcaatct tgcgcaggca
     5041 gtgcagggcg cagcactggt gattaccggg gaagggcgca tcgactcgca aacggcaggc
     5101 ggtaaagcgc cgctgggtgt ggcgtcggtg gcgaagcagt ttaatgtccc ggtgattggg
     5161 attgctggcg tattgggtga tggcgtggaa gtggtgcacc agtacggcat tgacgcggta
     5221 ttcagcattt tgcctcgtct ggcaccttta gccgaagtgc tcgccagcgg tgaaaccaat
     5281 ctcttcaaca gcgcgcgaaa tattgcctgc gccattaaaa taggtcaggg aattaaaaac
     5341 tgacccttac ctttaaagcg gatgcgattt atttcgcatc tttttttacc tgaatttact
     5401 acaaaaaaag ccacggtata aaccgtggca aaatccaaca tagctaaaaa taatcaggcg
     5461 agtggtatga cttaaatctc tacgtcgcgg ttgcaatctt tcgagtaaat atagctgaac
     5521 gcttcaccac gccctacacc ataaccagcc tgtaaagaat aagcgcccat aaagatgtaa
     5581 tcgccttttt tcaccgggat ccagttattg tcgaggttat aaaccccctg accggaaaga
     5641 atataggcac catgttcctg aacgtgtgtt tcgatataac cgtggctggc acctggcgca
     5701 aaagagagga tatgcatgtt catatcaaaa cctaactctt tgggcaggaa atccagcagg
     5761 atcacatcat ccataccttc atagtgaatg cgttccagtt cgctggcatt accagaaacc
     5821 agccacggtg catggccttc taccggaaca tagcggcgct tatataaaaa gatttggctg
     5881 tcttcggcct gggcgttaac aaacgtcatt aaggagcctg gtgggcaata aagatagcca
     5941 ccttcgctta aggcaaatgt tttgccttcg gctttggcag tgatatttcc agagatcaca
     6001 tacaggaacg tttcaatgcc ttcgccaccg aagccctgtt ggttgccacc gttttgatgc
     6061 agtgtgacca gataatcaac aaaagaggca cccagctttg gcgtggagag gattgtcgcg
     6121 tcacaatttt caaagcccgg aataatattt tttaccagac catccggggt taacagtgcg
     6181 aaattaccgt gtttaacaat cgcacggtta gccagtaaat catcgcggta accggtgacg
     6241 ttatttaaat atcccattta tgactcctta tttctgccag gcaagttgat aaagcatgag
     6301 tgccaacgtt ttgacccctt cggcaaggtc ggtaatattg gtgcgttccg ccgggttatg
     6361 gctgatcccg ttgatgctgg gaatgaaaat catgcaggtt ggtacgcgag gcgcgaaaat
     6421 ttgcgcgtcg tgcccggcac cactgtgcat cacccggtaa ttcagttttt ctctttcaca
     6481 caattctgtc agggtggcga ccagctcctt attcatcggc acgggttctt cgtccatcca
     6541 taaatcgata tcaataccaa tgtccatttc atcgcaaatc gcccgcatgt cgttttctaa
     6601 ctgttgggtg aaatcgcgca gcacggcagc gtcggtatga cgacaatcaa tggtgaacgt
     6661 ggttttaccc ggcaccacat ttaccgtatt cgggcgcggc tctactttgc caaaggtcag
     6721 aaccagcgga tcgcccatcc ttttcgcttt ttcgaccgac tgatggcaaa tgcgactgaa
     6781 agcgtaaact gtatcacgac gataacccat cggcgtggtg cctgcatggt ttgattcgcc
     6841 gttcagcgtt accgtataac gacgctgccc gacaattgca ttcaccacgc caattgattg
     6901 cccattactt tccagcacac agccctgttc aatatgcagt tcaacaaagg ctttaatatc
     6961 ctgacgcgga gttagtgggg cgttcggaag agtaaatccg caagccttca tcgcatcgac
     7021 aaaactattt cctttggcat cacagatatt ccgcacgtcg tcaggattcg ccagcccaaa
     7081 gatattttta ctgccccaga agacatacgg gaagcggctg ccttcttctt ctgccatcgc
     7141 caccacttcg accgtacgta gcggcgcgcc gtattgcgtt ttcagccagt caattgccag
     7201 ccacgccgcc agcgcgccga attgcccgtc gaggttaccg ccgttaacca cggtatcgat
     7261 atgcgaaccg ctcagaacca cttcttgtgg atattcggtg ccactcaggc gaccatataa
     7321 attccccact tcatcgaaac gtgtttccag cccgcttgct gccattcttt ttttaaattg
     7381 ctgctgagtt tccagccatt ccggcgaata aagtaaacgg gtcataccac ccgttgggtc
     7441 agcgccaaaa gaggaaagcc agggcagcgt ttcttctatg gcttgacgaa aatgtgtaat
     7501 cataagaaag tcctgtctca ataattattg cgcaaaggga tttttcgttt cgtatgacgt
     7561 gttataaagc gcgtcggaaa ttaaatactg gtaaatatca tcaacaattt cgatgccttc
     7621 gacggcggct ttgcgttgtt taatatcctg atcctgtccg ggataataaa cctgattaaa
     7681 accgggcgcg ggggtaatgg catttaattc gcgcatggtc tggctaagat gttgacgaaa
     7741 taatttgctg gagaagaaaa agttcgggtt aataacgata tgtaattgcc ccaaattacg
     7801 cccggcgtgt aaatcgtcat acatcgaact aacctgtcgc ccgaacggta agccgagtaa
     7861 gacgcctgag aggacgtcaa tcatcatcat caggccatac ccttttggcc cggcggcggg
     7921 gagcagagca tgtaccgcga acggatcggt tgttggtgca ccgtttttat cgaccgccca
     7981 ggtatccggg atagacatat tacgcgagcg ggcgtcgagc acttttcccc atgcctgtac
     8041 ggtagtcgcc atatcaaagg taaggatctc gtcgccttct cccggcgcgg caaaggccag
     8101 tgggttagta ccgtagtaaa tttccgcgcc gccaaacggc accaccattg gatcggactg
     8161 gcacatcgaa ataccaatta atccggcgcg ggctgcctgc tgcacaaaat aagagattgc
     8221 gccgctgtga cccatccggc tgataccgac caccgcaacg ccattttgct gggcggtttt
     8281 gatggcatgt tccataccca ttttcgccgc gacctgtccg gcggcattgt cggcatgtaa
     8341 aattgccgag cacggcccgg tttcctcaag acgaaactcc ggttcgcggt tggtgccgcc
     8401 ttttgaaatg cgttccgcgt agtattccac gcgcaccgcg ccatgagagt ggatccctct
     8461 ggcatcggcg taaaccaata cttcagccac ggttgcagcg tgctcacgtt ttaacccagc
     8521 ctggcagagt ttattctcaa ttagctggtg gagtgtttcc cgactgattt tcatctgtct
     8581 tcctttttaa cgacggtgtg aagcatgact gcaattaaca tacagggaaa atatctggat
     8641 tatgtgatcc agacaggcaa aaaaatatag ttagaattta tttgataatc cgctcacttt
     8701 taacctgatt tttaaaacaa ccacgcttat taaaaaataa tgcgtaatag cctggcggtt
     8761 atttgaattc ttttgttaat aattcctgtg tgatattcat caccttattt acttgttgtc
     8821 atcgataccg taatcgccac attaacactg ctcgtgcaat tgccatgggt gcaattttta
     8881 aggagttgtt atgatccacg cctttattaa aaaagggtgt tttcaggatt cggtcagttt
     8941 aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt gatgatgttt ccgtaatgat
     9001 gggtacgccc gccaataaag cgttattaga taccacaggt ttctggcatg acgattttaa
     9061 taacgccacg ccgaacgata tttgcgtggc aattcgtagc gaagcggcgg atgcggggat
     9121 cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa caactggcgc aggggtcagg
     9181 cagcagccag tccttgacgc aggtgcgtcg ctgggacagc gcctgtcaga aattacccga
     9241 tgccagtctg gcgctgattt cagtggctgg cgagtatgcg gcggagctgg cgaaccaggc
     9301 gctggatcgc aacctcaacg tgatgatgtt ctccgataac gtcacgctgg aagatgaaat
     9361 ccaactcaaa acccgcgcgc gggaaaaagg cttgctggtg atggggccgg actgcggtac
     9421 atcgatgatt gccgccacgc cgctggcttt tgctaacgtg atgccggaag gcaacattgg
     9481 cgtcattggc gcttccggta ccgggattca ggagctgtgt tcgcagattg cgctggcagg
     9541 ggagggaatt actcacgcga ttggccttgg cgggcgcgac ctcagccgtg aagtgggcgg
     9601 catcagtgcg ctgacggcgc tggaaatgct cagtgcagac gagaaaagcg aagtgctggc
     9661 atttgtttca aaaccacctg ccgaagctgt gcgtctgaaa attgttaatg ccatgaaagc
     9721 aaccggcaaa ccgacggtgg cgctgttttt aggttatacc ccggcagtgg cccgcgacga
     9781 gaatgtctgg tttgcctcct cgctggatga ggccgcacgc ctggcttgcc tgctttcacg
     9841 cgttacggcg cgacgtaacg caatagcgcc tgtcagcagc ggatttattt gcggtttgta
     9901 taccggcggt acgctggctg ccgaagcggc gggattactt gccggacacc ttggcgtgga
     9961 agccgacgat acccatcatc acggcatgat gctcgacgcc gatggtcacc agattattga
    10021 cctcggcgat gatttctaca ccgtcgggcg tccccatccg atgatcgacc cagccttacg
    10081 caaccagtta attgccgatc tcggcgctaa accgcaagtg cgcgtattgc tgcttgatgt
    10141 cgtgattggc ttcggtgcga ccgccgatcc tgccgcctcg ctggtaagcg cctggcaaaa
    10201 agcctgtgcc gcgcgttcag acaatcaacc actgtatgcc attgccacgg tgacaggcac
    10261 tgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg ctggaagatg cggggatcgc
    10321 ggtagtgagt tcgctgccgg aagccacctt gctggcggcg gcactgattc gtccgctttc
    10381 gcctgccact cagcaacaca caccgtcatt aatggaaaac gtcgccgtga ttaacatcgg
    10441 attgcgcagc tttgcgctgg agctacaaag cgccagcaaa tcggttgtgc attaccaatg
    10501 gtcgccagtc gccggcggca ataaaaaact ggctcgttta ttagaacgtt tgcaataagg
    10561 ggttcccatg tttacatcag tggcgcaagc caatgctgcg gttatcgaac aaattcgtcg
    10621 cgctcgtcca cactggctgg atgtgcaacc ggcttcttca cttatcagcg aactaaaccc
    10681 gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg caggagatga ccggacccat
    10741 gaaaggggcg tgcgtgggcg catgtctgtt cgaaggttgg gcgaaagatg aagcgcaggc
    10801 gctggcaatt ctggagcagg gggaagtgaa cttcattcct tgtcaccatg tgaatgccgt
    10861 cgggccaatg ggcggtatta cttctgccag tatgccgatg ctggtggtcg agaacgtgac
    10921 cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc ggcaaagtga tgcgttttgg
    10981 cgcttacggc gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt taatgccagt
    11041 attaagcgcg gcgctggggc gcatggagca cggtatcgat ctcacggcga tgatggcgca
    11101 gggcattacc atgggcgatg agttccatca acgcaatatt gcttcctcag cactgttaat
    11161 gcgtgcgctg gccccacaaa ttgctcgcct cgatcatgat aaacagcaca tcgcggaagt
    11221 gatggatttc ctcagcgtga ccgatcagtt cttcctcaac cttgcgatgg cttactgcaa
    11281 ggcggcgatg gatgctggcg cgatgatccg cgcaggcagc atcgtcacgg caatgacccg
    11341 caacggcaat atgttcggga ttcgggtaag cgggctgggc gaacgctggt ttactgcgcc
    11401 tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg caggagcagg cgaacccgga
    11461 tatgggcgat agcgcgatta ccgaaacctt tggtatcgga ggtgcggcaa tgatcgcagc
    11521 gcctggcgta acgcgctttg tcggtgcggg tggcatggaa gcggccagag cggtatctga
    11581 agagatggcg gaaatttacc ttgagcgcaa tatgcagttg cagatcccag gctgggattt
    11641 tcagggcgcg tgcctgggac tggacattcg tcgcgtggta gaaaccggca ttacgccact
    11701 catcaatacc ggtatcgccc ataaagaggc ggggatcggg cagattggcg caggcactgt
    11761 gcgggcaccg ctggcgtgtt ttgaacaggc gctggaagca ctggctgaaa gcatggggat
    11821 tggttgagga acgcgcaatg acgatcatcc atcctctgct tgccagccgt agtgcaccga
    11881 attatcgcca gtcctggcgg ttagcgggag tgtggcggcg ggcgattaac ctgatgacgg
    11941 aaagcggcga actgttaacg ctgcatcgtc agggtagtgg tttcggcccc ggaggatggg
    12001 tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt atgcggcaat gaacgaccac
    12061 aggttgtggc tcaagggatt cgcctcgggc gtttcacggt taaacagcca cagcgttatt
    12121 gtttgctgcg tattacgccg cctgcgcatc ctcaaccgct tgcagctgca tggatgcaac
    12181 gcgcggagga aaccgggctt ttcgggccac tggcgttggc ggcaagcgat ccgctgaccg
    12241 ctgaattacg ccagtttcgt cactgttttc aggccgcgct caatggcgtt aagaccgact
    12301 ggcggcactg gctgggtaaa ggccccggat taacgccgag tcatgatgac acgctgagcg
    12361 gaatgctgct ggcggcctgg tattatggcg ctttagatgc gcgctccggt cgccagtttt
    12421 ttgcctgttc cgacaatctg caactcgtta ccacagcggt gagcgtcagt tatttacgtt
    12481 atgccgcgca aggatatttc gcctcgccac tcctgcactt tgttcatgct ctgagttgcc
    12541 cgaaacgtac cgctgttgcg attgattcgc tgctggcgct ggggcatacg tcaggggcag
    12601 atacactgct ggggttctgg cttggtcaac aattattaca aggaaaacca tgaaaacact
    12661 ggttgtagct cttgggggca acgccttact ccagcgcggt gaggcgctga cggcggaaaa
    12721 tcaatatcgc aatatcgcca gtgctgtacc cgcgctggca cgcctggccc gttcttatcg
    12781 gctggcgatt gttcacggca acgggccgca ggtggggctg ctggcattgc agaatctggc
    12841 gtggaaagag gtagaaccgt atccgctgga tgtgctggtg gcagaaagtc aggggatgat
    12901 tggctatatg ctggcgcaga gtttgagcgc acagccgcag atgccgcccg tgacgacggt
    12961 gctgacgcgc attgaggttt cgcctgatga tccggcgttt ttgcagccag agaaatttat
    13021 tggtccggtt tatcagccag aagaacaaga ggcactggaa gcggcttacg gctggcagat
    13081 gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct ccgcaaccgc gtaaaattct
    13141 cgacagcgaa gccatcgagt tgttgctcaa agaggggcat gtggtgattt gcagtggcgg
    13201 cggcggtgtg cctgtgacgg aagatggggc agggagtgaa gcggtgattg ataaagatct
    13261 cgctgctgcg ttgctcgctg agcagattaa cgccgatggg ctggtgatcc tcactgatgc
    13321 tgatgcggta tatgaaaact ggggaacgcc gcagcaacgc gccattcgcc atgccacacc
    13381 ggatgagtta gcgccatttg ccaaagccga tggttcgatg gggccgaagg taacggcggt
    13441 gagtggttat gtcagaagcc gtggtaaacc cgcgtggatt ggggcgttat cgcgaattga
    13501 agagacgctg gcgggcgaag cggggacctg tatttcgctg tagtcgtagg cattagacat
    13561 ttgtgcctga tgcgacgctt gacgcgtctt atcaggccta caaccggtgc cgcatccggc
    13621 aattggtgca caatgcctga tgcgatgctt gacgcatctt atcaggccta caatgggtac
    13681 cggatcggta ggccggataa ggcgtttacg ccgcatccgg caagaataga gcaccagtta
    13741 accgaactta ctctgcgccc aaatcacgcc gctggcatat tccggcggca gcagcgggat
    13801 taaggcttcc agcgtcgcag tcagacgcga tgtgtcgctg tcggtcaaat tcagatgccc
    13861 cactttacgc cccggacgga cttctttgtc gtaccagtgc agatgcacca gcggcagttt
    13921 cagccagtca taattcgcat cgctaccaat cagattgatc atcaccgacg gattattcac
    13981 cactggttgc ggtaacggca gatcggtaat cgcccgcaga tgcagctcaa actggctgat
    14041 gctggcaccg ttttgtgtcc agtgaccgct gttatgcaca cgcggtgcca gttcgttgat
    14101 caacagacct tgcggggtga caaaacactc catcgccatc acgcccacat agcccagctc
    14161 ctgcataatc gccgaaagca tctcttcggc ttgcgcttgc tgctgcgcat tggcctgcgg
    14221 gaaagcgacg ctggtgcgca aaataccgtc ctgatgcagg ttatgcgtca gcggataaaa
    14281 cacggtactg ccatcaaagc cgcgcgcgcc aaccagcgac acttcaccag agaagtttat
    14341 gccctgctcg acaatacatt cgccgtagca ttcagccggt aactgctcgg tttcatcggc
    14401 gcgtaaacgc cattgaccgc gaccgtcata gccaccagtg cgacgcttaa caatcgccag
    14461 ctcacctaaa cgatcaaaca ccgcaggcca ctcgctgcga tcggcaagta actgccacgg
    14521 cgcggtcggc aggtggagct tatcgaaaag ctgcttctga gtcagacggt cagcaataat
    14581 cgggaacaca tcgcggttca caaaggccgg atgacgcgcc agctcgcggg ttaatgcggt
    14641 ttccggccag cgttcgatct cagcggtaat cacgctttgt tgaaaaggca ccgccgccgg
    14701 ttcagcgtcc agcccgaccg gccagacagc aatgcctaac ggttcgcctg cctgacgcag
    14761 catacggcct aactgcccgt taccgaggac gcaaacctgt ttcatgccgc acctcgcggg
    14821 tccgggtttt ccagcacttc gtcggtctgg gctttgcgcc agtcattcag acgctggtgt
    14881 agctctttat cgtgagtcgc gagaatttgt gctgccagta atgccgcgtt tgccgcgcca
    14941 gctttaccaa tcgccagcgt acccaccgga atgccgcgcg gcatttgtac gatggagtag
    15001 aggctatcga caccgctcag tgcggcgctc tgtactggca cgcccagcac cggcaccagc
    15061 gttttggcgg caatcatgcc tggcaggtgc gctgcgccac ctgcgcccgc aataatcacc
    15121 tgataaccgt tctcttcggc gctttcggcg aagctgaaca gtttatcggg ggtgcggtga
    15181 gcagaaacca cttcaacgtg gtgcgggaca ttcaggattt cgaagatttc ggcggcgaac
    15241 tgcatggtag cccagtcgct tttggacccc atcacgatgg cgacacgcgc cggattattg
    15301 cgggaagaca tgcgtcttaa aactcctgtg gtgcacaact ctcggcttta gagggcacag
    15361 agaatagcac ggaaagagag caaggaaaac ggttgcgtgg ctgtgaaatc agcaaagttg
    15421 tgggtttttt aaaacggaaa atgaatcagc tcaacgtcat ccgccgtgac tttcaccatc
    15481 gaaccttccg tatgccaggc accgagtacc acgcgaaaag caggttgctg gttggcgata
    15541 agttcatgta ccgccgggcg atgggtatgt ccgtggatca gccattgcac ctgatgtttt
    15601 tccatcgcac tgactaccgc gttttggtta acgtccatga tcgccagcga tttactgctg
    15661 ttggcttctt tactgttcgc tcgcattcgc gcggcaatgc gtttgcgcac aaacaacggc
    15721 agggcgagga atagcatttg cagccagggt ttgtggactt tggcgcgaaa agcctgataa
    15781 cccgcgtcat cggtgcacag cgtgtcgcca tgcataatca acacccggcg accataaagt
    15841 tcgagcacct tttcttccgg caataatgtc atgccgcttt cacgggcaaa gcgtttgccg
    15901 agcagaaaat cacggttgcc atgaatgaaa taacagggaa cgccggaatc ggacaccgct
    15961 ttgatcgctg ccgccatctg gcgatggagt gggttgggat cgtcgtcgcc aatccatgct
    16021 tcaaacagat cgccaagaat atacagcgcg tcggccttgc gggcttcccc cgctaaaaaa
    16081 cgcagaaaac cggcggtgat cgccggttct tccacgcaga gatgaagatc tgcaataaag
    16141 agtgtcgcca cgattactcg ctaacggtca cgctttcaat gataacgtct tcttttggca
    16201 cgtcctggtg cataccgcta cgaccggttg caacaccttt gattttgtct accacgtcca
    16261 tgccttcaac cacttcagca aacacgcagt agccccaacc ttgcaggctt tcgccagaga
    16321 agttcaggaa gtcgttatca accacgttga tgaagaactg tgcagttgca gagtgcggag
    16381 cctgagtacg ggccattgcc agcgtaccac gggtattttt cagaccgttg ttggcttcgt
    16441 ttttgatcgg ttctttggtg gctttttgtt tcatgcccgg ttcaaaaccg ccgccctgaa
    16501 tcataaagcc gttgataaca cggtggaaaa tggtgttgtt gtaaaaacct tcgcggcagt
    16561 agtccaggaa gtttttaact gtttcaggtg ctttatcgtc aaaagttttg atgacaatat
    16621 cgccgtgatt ggtgtggaaa gtaaccattt ttgcatcctg ttccgtttga ttggtgcttc
    16681 aacccagttc gggtcatata tagggtggtg ttatagcata accgcacgat cggatcatca
    16741 cgcaatgtat gctgattcgc gcgggaaata tgggtattat acgcaactca attacccaca
    16801 catgtctaaa cggaatcttc gatgctaaaa atcttcaata ctctgacacg ccaaaaagag
    16861 gaatttaagc ctattcacgc cggggaagtc ggcatgtatg tgtgtggaat caccgtttac
    16921 gatctctgtc atatcggtca cgggcgtacc tttgttgctt ttgacgtggt tgcgcgctat
    16981 ctgcgtttcc tcggctataa actgaagtat gtgcgcaaca ttaccgatat cgacgacaaa
    17041 atcatcaaac gcgccaatga aaatggcgaa agctttgtgg cgctggtgga tcgcatgatc
    17101 gccgaaatgc acaaagattt tgatgctttg aacattctgc gcccggatat ggagccgcgc
    17161 gcgacgcacc atatcgcaga aattattgaa ctcactgaac aactgatcgc caaaggtcac
    17221 gcttatgtgg cggacaacgg cgacgtgatg ttcgacgtcc cgaccgatcc gacatatggc
    17281 gtgctgtcgc gtcaggatct cgaccagctg caggcaggtg cgcgcgttga cgtggtcgac
    17341 gacaaacgca acccgatgga cttcgttctg tggaagatgt cgaaagaggg cgaaccgagc
    17401 tggccgtctc cgtggggcgc gggccgtcca ggctggcaca ttgaatgttc ggcaatgaac
    17461 tgcaagcagc tgggtaacca ctttgatatc cacggcggcg gttcagacct gatgttcccg
    17521 caccacgaga acgaaatcgc gcagtccacc tgtgcccatg atggccagta tgtgaactac
    17581 tggatgcact cggggatggt gatggttgac cgcgagaaga tgtccaaatc gctgggtaac
    17641 ttctttaccg tgcgcgatgt gctgaaatac tacgacgcgg aaaccgtgcg ttacttcctg
    17701 atgtcgggcc actatcgcag ccagttgaac tacagcgaag agaacctgaa gcaggcgcgt
    17761 gcggcgctgg agcgtctcta cactgcgctg cgcggcacag ataaaaccgt tgcgcctgcc
    17821 ggtggcgaag cgtttgaagc gcgctttatt gaagcgatgg acgacgattt caacaccccg
    17881 gaagcctatt ccgtactgtt tgatatggcg cgtgaagtaa accgtctgaa agcagaagat
    17941 atggcagcgg cgaatgcaat ggcatctcac ctgcgtaaac tttccgctgt attgggcctg
    18001 ctggagcaag aaccggaagc gttcctgcaa agcggcgcgc aggcagacga cagcgaagtg
    18061 gctgagattg aagcgttaat tcaacagcgt ctggatgccc gtaaagcgaa agactgggcg
    18121 gcggcagatg cggcgcgtga ccgtcttaac gagatgggga tcgtgctgga agatggcccg
    18181 caagggacca cctggcgtcg taagtaattg cgctgttgcc ggatgcgagt tttcgcatcc
    18241 ggttatcgcc tgcgccacca caacattccc ataagcacca tccccggcaa ccacacccac
    18301 atcaattcag aaattatcac ctgatgcccg tacggcgtgg tgtaacgaga caatgcaaac
    18361 ggcgcgactt taatcacctg ccagggagcg aaaaagcgtt catctgacca cggccacagc
    18421 cagccaacgc ccttaccgcc agtggttacc gaatccagca agctgtgcga tagcaacgag
    18481 acggttaaaa acagccagca gcgaatcagc ccagccctga accatcggcg tccaatcagc
    18541 acacataaca gcgggacaac aaacgcaaac accagcgaat gggtaaaccc gcgatgacca
    18601 aaaacattgc cgtaagcaac gccaaattta aacgacaata cgtcggcgtc gggcagcatc
    18661 gccaggatga ttccggcaaa taacagacgc ggagggatga ctttcgaacc caaccctaaa
    18721 ccaatgcata ggggaacggc ggcgtgcgta ataacggttg gcatgatggt cgcttcggca
    18781 aaatgtcgat gctatcagca tggatgaacg gggcgtagag ggcaaaagtc tgaaaagaga
    18841 accggcctgt tgatacaggc cgggaaaggg atcaggcaac aacctgtacg ctgtgacctg
    18901 caaaactcac tgtctgaccg gcgacgattt tgcagcgttt gcgcgtttca accgcaccgt
    18961 cgactttcac ctggccttcg gcaatcgcga ttttcgcctg cgcgccgctt tcgctccagc
    19021 cttccagttt cagcaagtcg cacagctcaa cgtgcggatg tttacctaaa gaaaatgtcg
    19081 ccatgttact catcctgtgg atcatgatat tcaacgcacg cctgtagcgt gttttcaatc
    19141 agcgtggcaa ccgtcatcgg gccaacgccg ccgggaacag gcgtaatgta tgaggcgcgt
    19201 ttagccgcgt cttcaaacac gacgtcgccc acaactttgc cattttccag acggttgatg
    19261 ccgacatcaa tcacaattgc gccttctttg atccagtcac caggaataaa gcccggcttg
    19321 ccgacggcaa cgatcaacag atcggcattt tctacgtggt gacgcagatt tttagtgaaa
    19381 cggtgagtca cggtagtggt gcaacctgcg agcaacagtt ccatgctcat tggacgaccg
    19441 acgatattcg atgcgccaat caccacggcg ttgaggccga aggtatcaat gttgtaacgc
    19501 tcaagcagcg tgacgatacc gcgcggggtg cagggacgca gacgcggcgc gcgctggcac
    19561 agacgaccga cgttataagg atggaaaccg tccacgtctt tgtccggatg aatacgttcc
    19621 agcactttga cgttatcaat acccgccggt aacggcagtt gaaccagaat gccatcgatg
    19681 gtgttgtcgg cattcagcgc atcgataagc tccaacagct ccgcttcgct ggtggtttcc
    19741 gggaggtcat aagagcggga gacgaacccg acttcttcac aagccttgcg tttgcttgcg
    19801 acataaattt gcgatgcagg gttactaccc accagcacaa cggccagtcc tggtgcccgc
    19861 agtccggctg caatacgcgc ctgaactttt tgagcaactt cagagcgcac ctgctgcgca
    19921 atcgttttac cgtcaataat ctttgctgcc atcagagaga ggattccatc tgttacgtag
    19981 gtcgaagggg atgcgcctat tttgtcagaa gcggggcgcg ctgtcaggtt tcgtttcaga
    20041 tttatcgcgt gaagcgacct cttgcgaagg ttaggcgcac cgtcgctgag gtcgctgaga
    20101 ttgaaagctt catttttcgt ccatgatggc gttgtaaatc tggaa
//
LOCUS       DAPPNJ010000073        20056 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000073 DAPPNJ010000000
VERSION     DAPPNJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.073"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(136..882)
                     /gene="cutC"
                     /locus_tag="RPM73_002185"
     CDS             complement(136..882)
                     /gene="cutC"
                     /locus_tag="RPM73_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDX6520541.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAKMKGIIERHQAK"
     gene            complement(896..1462)
                     /locus_tag="RPM73_002186"
     CDS             complement(896..1462)
                     /locus_tag="RPM73_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX6520542.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1678..3411
                     /gene="argS"
                     /locus_tag="RPM73_002187"
     CDS             1678..3411
                     /gene="argS"
                     /locus_tag="RPM73_002187"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDX6520543.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3588..4076
                     /locus_tag="RPM73_002188"
     CDS             3588..4076
                     /locus_tag="RPM73_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDX6520544.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4196..4588)
                     /gene="flhe"
                     /locus_tag="RPM73_002189"
     CDS             complement(4196..4588)
                     /gene="flhe"
                     /locus_tag="RPM73_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDX6520545.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4588..6666)
                     /gene="flhA"
                     /locus_tag="RPM73_002190"
     CDS             complement(4588..6666)
                     /gene="flhA"
                     /locus_tag="RPM73_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDX6520546.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGIVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6659..7807)
                     /gene="flhB"
                     /locus_tag="RPM73_002191"
     CDS             complement(6659..7807)
                     /gene="flhB"
                     /locus_tag="RPM73_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDX6520547.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8009..8653)
                     /gene="cheZ"
                     /locus_tag="RPM73_002192"
     CDS             complement(8009..8653)
                     /gene="cheZ"
                     /locus_tag="RPM73_002192"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDX6520548.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8664..9053)
                     /gene="cheY"
                     /locus_tag="RPM73_002193"
     CDS             complement(8664..9053)
                     /gene="cheY"
                     /locus_tag="RPM73_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDX6520549.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9068..10117)
                     /gene="cheB"
                     /locus_tag="RPM73_002194"
     CDS             complement(9068..10117)
                     /gene="cheB"
                     /locus_tag="RPM73_002194"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDX6520550.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10120..10980)
                     /gene="cheR"
                     /locus_tag="RPM73_002195"
     CDS             complement(10120..10980)
                     /gene="cheR"
                     /locus_tag="RPM73_002195"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDX6520551.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10999..12600)
                     /gene="tap"
                     /locus_tag="RPM73_002196"
     CDS             complement(10999..12600)
                     /gene="tap"
                     /locus_tag="RPM73_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDX6520552.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12646..14307)
                     /gene="tar"
                     /locus_tag="RPM73_002197"
     CDS             complement(12646..14307)
                     /gene="tar"
                     /locus_tag="RPM73_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDX6520553.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="RPM73_002198"
     CDS             complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="RPM73_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDX6520554.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="RPM73_002199"
     CDS             complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="RPM73_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDX6520555.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16945..17871)
                     /gene="motB"
                     /locus_tag="RPM73_002200"
     CDS             complement(16945..17871)
                     /gene="motB"
                     /locus_tag="RPM73_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDX6520556.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17868..18755)
                     /gene="motA"
                     /locus_tag="RPM73_002201"
     CDS             complement(17868..18755)
                     /gene="motA"
                     /locus_tag="RPM73_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDX6520557.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="RPM73_002202"
     CDS             complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="RPM73_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDX6520558.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="RPM73_002203"
     CDS             complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="RPM73_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDX6520559.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4788 a         5535 c         4900 g         4833 t
ORIGIN      
        1 ctgtcacccg ctcaacagat cgcggttatc gttcattttt tatactgttc agggataaaa
       61 aaaggcccct gttgaaattg caggggcctg gtacgagcaa gcatcatatt gggcgacatg
      121 atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg attcctttca ttttagcaac
      181 cgccgccccg tctacgacat aacgcgaata ctcgtccgcg tgtgcatctg atgacatgga
      241 caatccttga ttacgataac gcatcggtga agcttgccac gctcccgcgg agctatggac
      301 ttccagcact ccggcatcga ggaagtggtg caggttttct gcacggactc ctgctccggc
      361 cataatgatt ggagcatcac gatgggcaat aagttccata atttttgata aaccttgcag
      421 tgcgtctgat ttttgccctg atgtcagtac ccgggtaatg cccaattccg caagattatt
      481 gagtgtattt agagggttag cgcacatatc gaaggcgcga tgaaaagtca ctgccagcgg
      541 accggcagca gccattattt tttccattcg tggcatatcg acattcccgt caacatcgag
      601 aacgcccgtc accagtccag gaaaacctaa ttcgcggacc gtgcgcacat cctcaagaat
      661 ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg cgtgggcgaa ttatcggatg
      721 cacagggatc gtcacccgct ggcgcacgga tttcagtaca cccaacgacg gcgttaagcc
      781 cccctctttt ggggctgcgc ataattcaac tctgtctgcg ccgttttgct gcgccgttag
      841 cgcacattcc atgctgtaac agcaaatttc cagtaatgcc atttttgctc cttaattacg
      901 ctgactgctc gctggcaacg atctcttcaa tggaccacgg atgaaactta atagttgttt
      961 tgccatcggt gaccgccagc gtcggattcg gtaatcgctc atgctcacct tttggtgaac
     1021 tggttttaac tgagatcccc ggtaggctga gtccttcatc agaaagcaat gccagcgcac
     1081 gggcattcag cgtttctgga tcacccggca gaacgatttc aatatgttcc caaccttcgt
     1141 gtgggtaacg tttttccccg ggccacggta gctccacaat ggaaaactgc cagtgcgcaa
     1201 cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct gccattgatc atattttctg
     1261 acaaaagctc gccacactgt tcaaaaccgc gacgccagcg ctcagcggtg acgttttgat
     1321 ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat atccaacccc agacggcggg
     1381 aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga tgcaatatcc tgcagctcgt
     1441 cgatagattg ccagttcgcc ataatcactc ttcgtctttc agtaaaagcg ttaatttacc
     1501 ctgttgccct gtgccaacca accgctgatt tcacgccgct tctgatgcaa tagtgaaaac
     1561 ggcaatacgc cacgcgcacg ttgctgacga aaacagccat ttgcagtata ctcccgccct
     1621 aatttcttta actggtgcgg gcaatttttg ctcgcttcat caatgtaagg tattccggtg
     1681 aatattcagg ctcttctctc agaaaaagtc cgtcaggcca tgattgcggc aggcgcgcct
     1741 gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc agttcggcga ctatcaggct
     1801 aacggcatga tggcagttgc taaaaaactg ggtatggcac cgcgacaatt agcagagcag
     1861 gtgctgactc atctggatct taacggtatc gccagcaaag ttgagatcgc cggtccaggc
     1921 tttatcaaca ttttccttga tccggcattc ctggctgaac atgttcagca ggcgctggcg
     1981 tccgatcgtc tcggtgtttc tacgccagaa aaacagacca ttgtggttga ctactctgcg
     2041 ccaaacgtgg cgaaagagat gcatgtcggt cacctgcgct ctaccattat tggtgacgca
     2101 gcagtgcgta ctctggagtt cctcggtcac aaagtgattc gcgcaaacca cgtcggcgac
     2161 tggggcactc agttcggtat gctgattgca tggctggaaa agcagcagca ggaaaacgcc
     2221 ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac
     2281 gaagatgaag agtttgccga gcgtgcacgt aactacgtgg taaaactgca aagcggtgac
     2341 gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca ccatgacgca gaaccagatc
     2401 acctatgatc gtctcaacgt gacgctgacc cgtgatgacg tgatgggcga aagcctctac
     2461 aacccaatgc tgccaggaat tgtggcggat ctcaaagcca aaggtctggc agtagaaagc
     2521 gaaggggcga ccgtcgtatt ccttgatgaa tttaaaaaca aggaaggcga accgatgggc
     2581 gtgatcattc agaagaaaga tggcggctat ctctacacca ccactgatat cgcctgtgcg
     2641 aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt attacatcga ctcccgtcag
     2701 catcaacacc tgatgcaggc atgggcgatc gtccgtaaag caggctatgt accggaatcc
     2761 gtaccgctgg aacaccacat gttcggcatg atgctgggta aagacggcaa accgttcaaa
     2821 acccgcgcgg gtggtacagt gaaactggcc gatctgctgg atgaagccct ggaacgtgca
     2881 cgccgtctgg tggcagaaaa gaacccggat atgccagctg acgagctgga aaaactggct
     2941 aacgcggttg gtattggtgc ggtgaaatat gcggatctct ccaaaaaccg caccacggac
     3001 tacatcttcg actgggacaa catgctggcg tttgagggta ataccgcgcc atacatgcag
     3061 tatgcttaca cgcgtgtatt gtccgtgttc cgtaaggcag aaattaacga agagcaactg
     3121 gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc aactggcagc tcgcctgctg
     3181 cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca cgccgcatgt gatgtgtgct
     3241 tacctgtacg atctggctgg tctgttctct ggcttctacg agcactgccc gatcctcagc
     3301 gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg cacaactgac agcgaagacg
     3361 ctaaagctgg gtctggatac gctgggtatt gagacagtag agcgtatgta atcgattttt
     3421 cgtgagagta aagcctgatc agggttacct gatcaggctt ttttattgcc atctaaatgt
     3481 attctgaaaa tggacatgcc attgttttct gactgttgga taagaggcca gaagcgtaat
     3541 atccggcccc agggaaacga taacggttga atttaaggaa taccgcagtg tttaaatttc
     3601 ttgtattaac attaggcatt atctcttgcc aggcttacgc agaagatacg gttatagtaa
     3661 acgaccatga catttcagcc atcaaagatt gttggcaaaa aaattcagat gatgatactg
     3721 acattaacgt gatcaaatca tgcctgcgac aagaatacaa tctcgtcgat gcgcaattaa
     3781 ataaagccta tggtgaagct tatcgttata tagaacaagt gccacgcaca ggtgtaaaaa
     3841 aacctgatac cgaacaactt aacttgctta aaaaatcaca gcgagcctgg ctggatttta
     3901 gggacaaaga atgtgaatta atcctttcaa atgaggacgt tcaggattta agtgaccctt
     3961 attctgaatc agaatggctc tcatgtatga tcatacagac caatacgcga actcgccagt
     4021 tgcagctata ccataactct gaagattttt atccaagccc tttgacaaga ggataattca
     4081 catctttttg gcatgttttg ttgcaagcta ttcctgataa ataattgcaa caagacatcg
     4141 agcctttttc actgagttat taaacatact cgcgagcgcg taattttttt gtccttcagc
     4201 gataattcac aatcacttca ttacgttgta cctttagcgg tggaattaac cgcccaccgc
     4261 ctggcacttc ccagataaat cgcaacggtt ctgctgccgc tataccggaa aaggccacgg
     4321 tggttccgct ctgcccctct aattcgacac aacgagattg cgaacacaaa cgaacccgca
     4381 gtcctgaagg tgtcgggccg ataagctgat aacgccacgc taccagcgtc atcaatcctg
     4441 aagcaggttg tcgcgtagaa agaggcgcag acgacatcga ctcaccgcga tgatttagcg
     4501 taattcctac actacttgcc tgccacatcc cctccccggc ggcttgcacc agcagcggaa
     4561 acaataatat tgctaataag gttctcatta tttgccgcca attgtcgccg tcatgcggat
     4621 atgtcggtta tcagacagtt ccagattcga caggaccact aactgcggca agctgcggcg
     4681 caggaagcga gacaataatg gtcgcagcgc gtggttcacc aacaatactg gcggcgcacc
     4741 cagcatctcc tgacgggata gcgcttcctg agtttgcgcc agtaaacgat ccgccagccc
     4801 tggctccagt cctcccccgc cctgcagcgc ctgtagtaac aaacgttcca gcggtgtatc
     4861 gaggccaata acatggactt catctttgcc aggaaaccac tgctgggtaa tcgcccgtcc
     4921 caacgccacg cgcacgacgg cggttaattc atgtggatcg ctttggatgg gcgcatgttc
     4981 cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt actttttcat cgaggagatt
     5041 ttgcagcact ttatgcagtg tggtgagcgt gacgatgcca ggaacgagat cttccgtcag
     5101 ctttggcatc tcctgggcga cgcgatccaa cagctgttgc gcctcctgac gaccaaacag
     5161 ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg tgctggcctc
     5221 aaccactgtg tacccctgaa tctgcgcctg ttcttttagc gcactttcaa tccagatagc
     5281 attcaggcca aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg cagcggttcc
     5341 agggttaatc gccagccagc gccccggata agcatcacca ctgccaatct ccacgccttt
     5401 catcaaaatg cgatagcggg caggttgcag atccatattg tcgcgaatgt gcaccactgg
     5461 cggcagaaat cccatctcct gggcaaattt cttgcggata ctgcgtatac ggcccaacaa
     5521 ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga taacccactt ccattcccag
     5581 agaatcttcc agttgtacat cgttccacgt cgcttcgaca acggtattat tctctgccat
     5641 ttttaccggt ttgggttcgg caggcgcttt ttgttcgcgt ccgcgtatcc accaggccag
     5701 ccccagcaat ccggcagtga acagcaaaaa taccaggttc ggcattccag gcaccaggcc
     5761 gagtaaaccg agcacggcgg cgcttaacaa cataacgctt gggttactga aaagctgatt
     5821 caccatctgc tcgccaacat cctgatcggt gctgacacgc gtaacgatga cccccgcggc
     5881 ggtagaaatc accagcgccg gaatttgtgc caccagaccg tcgccaatgg tcaatagcgt
     5941 ataactttcc gccgcgtgtc ccatgctcat gccatgttgc agcacgccga ccagcaaccc
     6001 gccgacaacg ttaatgacca tgatgaggat cccggcgatg gcatcgccgc gaacaaactt
     6061 acttgccccg tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg agcggcgttt
     6121 tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg gcgtcaatcg ccatctgctt
     6181 acccggcata ccatcgagaa caaagcgcgc acccacttct gcgatacgcc cggcaccttt
     6241 ggtaatgacc ataaagttga tgatcacgag aatgacaaac accacgatac ctatagcgaa
     6301 attgccacca acgaggaagt gaccgaacgc ttcgaccacc ttccctgccg ccgccgcgcc
     6361 ggtatgccct tccatcaaaa tgatacgggt tgaagccacg ttaagtgcca gacgcaacaa
     6421 cgtagtaaac aacagaatgg tcggaaacgc agcaaactca agcgtgcgct gggtaaacat
     6481 cgccaccagc aacaccatga tcgacaaggc aatattgaag gtaaacaaca ggtcgagtat
     6541 gaatgcgggc agtggcagca ccatcatcga caagatcaac aggatcaaaa tcggtccggc
     6601 aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg cgcagcatcg cggccagatt
     6661 actcatgggt cggtttctcg ttaataaaat ccagggcttc cggcaccgga agatgagtag
     6721 gttgtacagg gcgctgtcca ccagccagac gccagcgttt cagttgccag acccaggcca
     6781 gcacttccgc caccgcggcg tacagttgac ccgggatttg ttgaccaatc tccgcatgtc
     6841 gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg gacgttattt tcagcgccaa
     6901 tttcacgaat gcgcagcgcg accagccctg cacctttagc gaccactttc ggtgcgctca
     6961 ttttgttttc gtcatactgc aacgctaccg aatagtgggt cgggttattg acaatgacat
     7021 ccgctttcgg cacatcggcc atcatccgac gccgtgcagc agctcgctgc atctgacgga
     7081 tccgcccttt aacatgaggg tcaccttcgc tttgtttgaa ctcatcacga atatcctgcc
     7141 gtgacatacg cagctttttc aggtggctga atatttggaa aaagacgtca aatcccacca
     7201 ttggaatgac accaagcacc accagcagtg cgcatagccc taccaaatcc atcgcattac
     7261 ccatggcggt aatcggagac tcggccatca agcgcatcat ctgcggccag tgatgccaga
     7321 gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa aattgcttta agcaactccg
     7381 cgccagtctg agccgagaac atccgtttaa tgcccggtag cgggttgagt ttggaaaact
     7441 tcggctgcaa ggatttgccg ctaaatacca gccctcccag catgaccgga gaaatgagcg
     7501 ccaccagcac cacgccgcta atcagcggca gcagcgccag catggcttct ctgatcagca
     7561 gaataatctg cccgaggatc agattcgggt cattgataat actgtgatca aaatgcagcc
     7621 cagcggagag catgcccgac aatcgacggg ccagcgacac accgccaaac cagataacac
     7681 taacgcccac cagcaaaatc agcagtgagg tcagttcacg ggaacgcggg atttgcccct
     7741 cttcccgcgc tttttctagt cggtgaggtg tgggggcttc tgttttgtcg tcgctctcgt
     7801 cagacacgtc gccaatcctg gaaagagtat taagcgtgaa tgatgccaga gcgcaaagcg
     7861 ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag ggcttttgct gccacacatc
     7921 aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt atcagaccgc ctgatatgac
     7981 gtggtcacgc cacatcaggc aatacaaatc aaaatccaag actatccaac aaatcgtcca
     8041 cctgatcctg actggctacc acaccggctt tgctggtatc gacctgaggt ccattaagca
     8101 aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc aacagcacca
     8161 tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaatg acctgcccgg
     8221 tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta gtaaagctgg
     8281 tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca cgggcgtcgg
     8341 caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct tttgctgatt
     8401 tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc cgctccgcag
     8461 cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg atggcttccg
     8521 ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc agcatacgcg
     8581 tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca ggtttgattg
     8641 atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt gttgagtttt
     8701 tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc cgcttgcgcc
     8761 gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg caatgccgac
     8821 atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt gggcatgttc
     8881 cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc gtcgacgcca
     8941 tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg cactatgcgt
     9001 cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc catttcacac
     9061 tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccaacatt tgctggctta
     9121 cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg cgcggcatgc
     9181 cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc tgacgcatcg
     9241 ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca accgcattac
     9301 gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga tgacggttaa
     9361 ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc actacgcgac agctccatat
     9421 gccgatcgcc cggcgcgata taggcatgtc ccggcaaaac acgttctccg tcttcggctt
     9481 ctttaacccc gatctgacaa agcttattaa gtctgtcggc aaaagagcgg gtgaaaccag
     9541 gcggcatatg ctgggtaatt aacagtgccg ggctggaaag cggcaacggt tgcagtacgt
     9601 gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt tcagaactca
     9661 acaacggccc tgccttcagc gttgtcggtg ccgacaatgg cttatgtgct gcaaggctcg
     9721 cctttgctgc tgtacgcacc ttttcagcaa tcatttcgct atacgccagc attccttcgc
     9781 gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc gcgcgcagcg
     9841 tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt ggacgcaaac
     9901 gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca tccagcgtca
     9961 gcacatcggg attgaatttc ttaatcaagt cacgcgcgac cagcggatca ggcgcggtcg
    10021 ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg cgcatcagtg
    10081 ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac ttagcgcata
    10141 caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt cagagtgacc
    10201 cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa tctcctgctg
    10261 ggtattttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa acggccccgg
    10321 cacggtgtac tgtttcgcca gtagattcag cggggcaaaa tcaacatagt tcgccagctc
    10381 ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat accgttgcag
    10441 ttgctgcggc gtcaggtttt tcaactcttc atggcgataa ataccgcttc tggctttttc
    10501 cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggcg cggtgcccaa
    10561 tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg ccggtcgaag ccgccgcgct
    10621 ccatacgcga tactcgccag aacggcgacg tgcatgatcc gcgagcagag ggaaatggtg
    10681 tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg cctgccactc
    10741 accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg tcagtcccag
    10801 cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt cagccagaac
    10861 gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt cggaaagcgc
    10921 caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag atgaagtcat
    10981 agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg ccgactcatg
    11041 tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa gatggtcggc
    11101 ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg cgttctgctg
    11161 agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc gctgttgttc
    11221 ttccgacgca gaggcaattt ctcccataat gtcgttcacg cgggtcaccg aactgacaat
    11281 atcgatcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga cacgattgac
    11341 tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc tggcaagatt
    11401 gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc gagccgcttc
    11461 cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga taacgctgat
    11521 aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag tcatggtact
    11581 gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt ccgacgcctg
    11641 tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac tggcggccgt
    11701 ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat tgcctgcgac
    11761 aatctccgcg ataccaatgt gcatctcctg gcttccctta cgcacatcac tcaccgtccc
    11821 acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct cattacgacc
    11881 atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat ggctaccgat
    11941 aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc tgatgtagat
    12001 cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt tacgctggct
    12061 ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct caaagttaac
    12121 gtcaaacgca ttctgtgatt cctgcaccgg cgcagtgagg aaatccgaaa gttgattact
    12181 ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac ttttctccgt
    12241 ctctttttgc aacgccctga cgtgctcgtt gcccgcagtc atcgccataa aacttttaaa
    12301 cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt taatgtcatc
    12361 cgccggatag ctaagtgccg tcagagtgcc cgctttgttc agcgcggtac tggcctgtaa
    12421 cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct cgacctgatt
    12481 cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc caatctgcaa
    12541 gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat
    12601 caacgctccc catcaggcgg caatgaccgc gttagtaaat actcgtcaaa atgtttccca
    12661 gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg
    12721 acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga acgcggaaac
    12781 cgcttgcgtt aaacgactcg cctgttcttc cagcgcagcg gcggcggcag ctgattcctg
    12841 caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg
    12901 atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa tgtcagtcac
    12961 gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga ccagcaccga
    13021 accggtatca acgcgtgaga cggagtcttc aatgagggct ttgatctctt ttgccgcctg
    13081 ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac cacggccctg
    13141 ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag tctggaaggc
    13201 aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg cgatctcatg
    13261 catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact
    13321 ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggtgag
    13381 ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag tacgggagga
    13441 aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc
    13501 gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag
    13561 gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac caccggcgat
    13621 ttcgcgaatg tgagcaataa tttttgccag cggagtaagc aacatacggc gaatgccgta
    13681 ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc
    13741 aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag
    13801 ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg gctgagcgaa
    13861 ataagctcca gtattgccat aatctagata atcaatcagt tcagttaacg ctgtgtaata
    13921 gtttttatat ttttcatcaa tattacgact ggtagcgacc atttcaggta acggtgccat
    13981 gcttttgaat tttttataat gcgtcgctgc ctgcgccaat gttttcctgg cgctatcgag
    14041 caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc gtaccgctga
    14101 acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca gctcgccctg
    14161 ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa gggaagaaaa
    14221 aaacagactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag
    14281 cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt
    14341 atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat tgaaataaaa
    14401 aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga attacgccac
    14461 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    14521 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
    14581 cgtcacggca aattctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    14641 aacgccgtca accacgatgc caaccacccg ttgtccgaga ttcaggacga taactaccgt
    14701 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    14761 aataaccccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    14821 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
    14881 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    14941 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    15001 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    15061 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    15121 accacaacct ggtgctgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
    15181 ctttgtaaga tcacaacaat tccctgggtg gcttcggttt tcgcgcccgc gacgttgaac
    15241 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
    15301 ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac agcattcagc
    15361 ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccaacgtc
    15421 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcgacatga
    15481 ccgcccatct cctggatatt acgtttaacg acatccatgc cgacgccgcg cccggagacg
    15541 tcggtgacct gctctgccgt ggagaagcca ggtgcaaata tcagcatcgc gacttcgtcg
    15601 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccaa aattcgctca
    15661 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    15721 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
    15781 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct
    15841 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
    15901 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    15961 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    16021 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    16081 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
    16141 gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc cacgcggccg
    16201 gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt tggcgagact
    16261 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    16321 tcatcttcgg cgatgtcgcc cggtaatatt gccgagagcg aatccgcccc tttcaccacg
    16381 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gccggccttc
    16441 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tctgctcatc ttgcggttca
    16501 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcaccttt cgcttctaat
    16561 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    16621 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    16681 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    16741 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
    16801 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    16861 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    16921 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
    16981 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    17041 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    17101 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgacgac
    17161 acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc
    17221 ccgatcggca gaaagctccc agttgctgta acctttctca ccactggcgt atgggaaatc
    17281 atcggtatga cccgaaaggc taatacggtt gggaatacca ttcagaacgg gcgcgatggc
    17341 gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttttaa acatcgggcg
    17401 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    17461 tggacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat caccccgcaa
    17521 tttccgcaat cgactttgct ccatgcgttt tttcaactct tcgatgttcg gctgcttatt
    17581 cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc tttcactatt
    17641 agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat
    17701 ctgaatcagc tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
    17761 catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    17821 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    17881 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    17941 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    18001 taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt gctggtttcg
    18061 gcgcttttct gacgtaaaac agtcgctaat ggggaaataa atccgtaagc caataaaatg
    18121 ccgaggaaag tccccaccat cgcatgtgcg ataagcgcac ccagctcggc ggcaggacga
    18181 tcggctgaac ctaacgcgtg aacgaccccc attacagccg caacaatacc aaacgccgga
    18241 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    18301 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    18361 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat gcgtgggtag
    18421 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    18481 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    18541 ttggtgtatt tggagcgacg aaacagcaac ggcagcgcct tcagcgtgcc tttaatcgct
    18601 ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat caccagttca
    18661 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    18721 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    18781 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa agcagcggta
    18841 cgtcgttacc gctgctggaa tgttgcgcct caccgtatca gttaaacagc ctgtactctc
    18901 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    18961 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    19021 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    19081 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc ttctgcttgt
    19141 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    19201 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    19261 caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg gcttccgcgc
    19321 agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct ttccagcatc
    19381 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    19441 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    19501 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    19561 caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    19621 aaaccagttg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaacg
    19681 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    19741 tcaaacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    19801 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtttcaccgc
    19861 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    19921 aagctgcaat aagcagaacc atctttttgg tttaatatgt ccttacaaat agaaatgggt
    19981 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    20041 atacaacgga gggggg
//
LOCUS       DAPPNJ010000074        20028 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000074 DAPPNJ010000000
VERSION     DAPPNJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.074"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            551..1267
                     /gene="nanC"
                     /locus_tag="RPM73_002204"
     CDS             551..1267
                     /gene="nanC"
                     /locus_tag="RPM73_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDX6520560.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1287..2393
                     /gene="nanM"
                     /locus_tag="RPM73_002205"
     CDS             1287..2393
                     /gene="nanM"
                     /locus_tag="RPM73_002205"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDX6520561.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2458..3438
                     /locus_tag="RPM73_002206"
     CDS             2458..3438
                     /locus_tag="RPM73_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDX6520562.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDKFVEAILQFWREK"
     gene            complement(3446..3568)
                     /locus_tag="RPM73_002207"
     CDS             complement(3446..3568)
                     /locus_tag="RPM73_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6520563.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKPN"
     gene            3650..4522
                     /locus_tag="RPM73_002208"
     CDS             3650..4522
                     /locus_tag="RPM73_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6520564.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVEWHRKEVFRG"
     gene            5456..6595
                     /locus_tag="RPM73_002209"
     CDS             5456..6595
                     /locus_tag="RPM73_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDX6520565.1"
                     /translation="MSTLKPAVFSFFSGSGFLDLGFENSGYEVVFVNEFHPPFMDAYK
                     HSRAVMNKPIPRFGYAEESIEEIDHKKLKDNIKALKKEKRLIGFIGGPPCPDFSVAGK
                     NKGRDGENGKLSQSYIDIIVEMKPDFFLFENVKGLYRTLKHREFFEHLKEQLMDVGYV
                     LHERLINSIEYGAPQDRERIILLGFQNKNLTKSFNWSGRLYPDRSAFGYNWPTTNEFV
                     EDSTLVMPESLPEELTVDYWFRKNNVSEHVNAKHVFTARAGLVRFKAVAEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKEFELPSYMTLSNMFKT
                     VGNGVPYLAAFGIAKSILEHLGIKDEFNSNESCECDSKSSQKQAV"
     gene            6546..7607
                     /locus_tag="RPM73_002210"
     CDS             6546..7607
                     /locus_tag="RPM73_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDX6520566.1"
                     /translation="MNLVNAIANLPKNRQFDYVNESNAGKIVIDSIKLPEGPIRFKRF
                     NPTKGETLRDAKIDSISSQMLWRLANAIQEDAPINLDRVFGASYNSRSVIESLLAHTP
                     QFYWCKLDRLEVINTKQTIKKGHKHLIYLPNSPHENGKLSEYKTNIVISEMNTEVVYQ
                     SVDSETIRPVEGMSIEESRRHAQIQIALVKIGHHLGYRTWVAANDRGLKYNGKAIAQM
                     DGVIDSLSNEKVLSSYSEAIDGARLIDCIWFRNGKLMPAVMEIEHSTGIISGLTRMKN
                     FCDYAPRLQDIRWTIVAPDEYREKVIEFANRPQFRELDTKFFPYSAVEELYSLCERRK
                     PQGITDKFLDSFMERCIKH"
     gene            complement(7637..8288)
                     /locus_tag="RPM73_002211"
                     /pseudo
     CDS             complement(7637..8288)
                     /locus_tag="RPM73_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8386..8470)
                     /locus_tag="RPM73_002212"
     tRNA            complement(8386..8470)
                     /locus_tag="RPM73_002212"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8434..8436),aa:Leu,seq:caa)
     gene            8666..9685
                     /gene="ahr"
                     /locus_tag="RPM73_002213"
     CDS             8666..9685
                     /gene="ahr"
                     /locus_tag="RPM73_002213"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDX6520567.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            9815..11317
                     /locus_tag="RPM73_002214"
     CDS             9815..11317
                     /locus_tag="RPM73_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDX6520568.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(11436..12518)
                     /gene="lptG"
                     /locus_tag="RPM73_002215"
     CDS             complement(11436..12518)
                     /gene="lptG"
                     /locus_tag="RPM73_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDX6520569.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(12518..13618)
                     /gene="lptF"
                     /locus_tag="RPM73_002216"
     CDS             complement(12518..13618)
                     /gene="lptF"
                     /locus_tag="RPM73_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDX6520570.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            13885..15396
                     /gene="pepA"
                     /locus_tag="RPM73_002217"
     CDS             13885..15396
                     /gene="pepA"
                     /locus_tag="RPM73_002217"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDX6520571.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            15530..15973
                     /gene="holC"
                     /locus_tag="RPM73_002218"
     CDS             15530..15973
                     /gene="holC"
                     /locus_tag="RPM73_002218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDX6520572.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            15973..18828
                     /gene="valS"
                     /locus_tag="RPM73_002219"
     CDS             15973..18828
                     /gene="valS"
                     /locus_tag="RPM73_002219"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDX6520573.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(18884..>20028)
                     /locus_tag="RPM73_002220"
     CDS             complement(18884..>20028)
                     /locus_tag="RPM73_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDX6520574.1"
                     /translation="GTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANMEVNG
                     QRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLR
                     YQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVII
                     SVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAFLA
                     LLPFLAVACYIIIDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVST
                     SYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIW
                     MIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
BASE COUNT         5477 a         4737 c         4981 g         4833 t
ORIGIN      
        1 gtgttcgtct ggcttcacat ggcatcttct tcttagaaaa atatcgacat attttgtgac
       61 acgaattgca aatctggttt tgttgtatgg attgcgtgat ttttgatctg gtataacagg
      121 tataaaggtg caccaagata gtcaatgaga cagggcctct cgcaatctat ggcaaacatc
      181 acttcagttc tttctcatcg ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa
      241 tgagatcaac agacattcta ttttatgact ctgggtaaaa tggattgagt aagtgatata
      301 gcttacgaac attcaaatca attaaacatc agaagagatt ttatactcag gtatttaatc
      361 tggatctctg tttatttaaa taatgtgaaa agagattttt cacaggagac attatacaaa
      421 aaaatataaa atacagctac cggttgccaa agacactata agcctggcaa aaaaatatta
      481 cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg
      541 agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc
      601 cccattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca
      661 cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat
      721 ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga
      781 tgttcaggtt gaagttaatt acgcgattaa acttgatgat caatggacgg tgcgcccggg
      841 aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg
      901 ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg
      961 acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt
     1021 cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca
     1081 gaacgattat cgctatgcaa accataagaa atgggcgacg gaaaatgcat ttgttctaca
     1141 ataccatatg acgcccgata ttacgccata catagaatat gactacctgg accgtcaggg
     1201 tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa
     1261 actgtagtag acaggagaca gtcacaatga ataaaacaat aacggcgctt gctatcatga
     1321 tggcttcatt tgccgcaaac gcgtctgtat tacctgaaac tcctgtgcca tttaaaagtg
     1381 gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca ggtacggcat
     1441 ggtacaagct ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg
     1501 gtggaccaag agatcaagca acctcggcat ttattgatgg caatctgtat gtgtttggcg
     1561 gcattggcaa aaatagcaag ggcttgactc aggtatttaa tgacgtacac aaatacaacc
     1621 ccaaaaccaa tagttgggtt aaattgatgt cgcatgcacc gatgggcatg gcgggtcatg
     1681 tgacttttgt acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca
     1741 atggctattt tgaagatctc aacgaggctg gaaaagattc agcaactata gacaagatca
     1801 atgcccatta ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt
     1861 ttgatccctc aacacagcaa tggagttacg ctggcgaatc tccctggtac ggaacggctg
     1921 gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag
     1981 gattgcgaac ggatgccgta tttgaacttg atttcaccgg taataattta aaatggaata
     2041 ggcttgctcc cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg
     2101 attctcttat atttgccgga ggggccggat tcaaaggttc acgagaaaat taccagaacg
     2161 gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cgctgatatt catctttggc
     2221 ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat
     2281 tgccctggaa taatagtcta ttgattattg gcggtgaaac tgcaggcggc aaagcggtga
     2341 cggattcagt tttgatctct gtgaaggata ataaagttac agtacaaaac taacgcttca
     2401 gggccccagt aaaggggccc tgctatcaat ttaccatttc aattacgcga aaattatatg
     2461 gacgcaataa tatcgccaga ttattactat gttcttaccg ttgctggtca gtctaatgcc
     2521 atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca tcccagaatt
     2581 aaacaattag cgagatttgc gcatacgcat cccggaggcc cgtcatgtca ctttaacgac
     2641 attattccac tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct
     2701 ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg
     2761 aaattactgc cctctattcc tgataatgca ggggttctca tcgttccatg ttgtcgtggc
     2821 ggctcggctt ttatcgcggg cagcgaaggg acatattcag aacggcacgg agccagccat
     2881 gatgcttgtc gttggggatc ggatactccg ctataccagg atttagtcag cagaacgcga
     2941 gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa
     3001 tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca tatggttgaa
     3061 gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac tgacgcgccg
     3121 tggttttgcg gcgataccac ctggtactgg aaagaaaatt tcccgcattc gtatgaagct
     3181 atttatggca attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa
     3241 caaggtgaaa gaggactgac gaacgcgcct gatgaagacc cggacgattt aagcacggga
     3301 tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat
     3361 ttcagcgcgg cagcccgtcg ggggattatt tctgacaagt ttgtagaagc aattttgcag
     3421 ttttggcgcg aaaagtgagc gttcattaat ttggtttatt cgcatccatc agcgctttta
     3481 actcctccgg cgtaaacggt ggtttgcgcc ggtgcagcat cgcatggcaa ttagggtaca
     3541 caggcagcaa aaaaacaaat gagatcaatg taatatatct tcacttgcag gcttgaacag
     3601 cagaaaattt gcgagcatga tcgcaattat ttcactgcca gggatatcaa tggcttccag
     3661 taaatcgcta cagcaggcaa ttgccaatat aaaaatctgg cataagggtg aacagcgcgc
     3721 accgcataaa ccattgttat tgctatatgt attagcggga tacctgaatg gacatccacg
     3781 ccttttcgat tatggatcgg aaatctacga acctctgcac agtttactgg aacgttttgg
     3841 gccacagcgt tcacagtacc gacctgatat gccgttctgg cgattacaag gcgacggatt
     3901 ctggcaatta cacaatgccg agctttgctc aacagcaggg agcagccgac aaccgccagt
     3961 gaaggaactg actgaatacc acgttgcagg cggtttcgat gaacagcact acgccctggt
     4021 aaccggcaat aaaaaactca tcaatactct ggcgcaacag atactcgaag cgcacttcac
     4081 cgagagtatt caggaagaaa ttgctgacga gctgggtttt gatctccagc aaatccgtaa
     4141 acagcgcgac ccgttatttc gcaaaaatgt attacgcgct tataactacc agtgcgcgat
     4201 atgtggcttt aatatgcgcc acgacgacac caccgtcgcg ctggaagcgg cgcatataaa
     4261 atggaaacag cacggcggtc cttgtgaaat tcctaatggc ctggcgcttt gcgcaattca
     4321 ccataaagcc tttgataaag gatcgattgg cctggacgaa aatatgcggg tactggtatc
     4381 agatgcggta aacggcggag ggatcgttga gagattattc tgggattttg acgggaaaac
     4441 tattgcgttg ccgcaggtgc gtaaaaatta cccttatgag gggtttgtag agtggcatcg
     4501 gaaagaggtt tttagggggt gaggttgata actatcgcag tatgttcaat attgctgaca
     4561 taatcagtgc aggatcttgc cgctagttta caactcgcct tcgatagcgg cgcaaaacga
     4621 gttctgttgc cgatatcctc ggctatggat attccaacag ttccggcaga gttgtttacc
     4681 aagtttcagg taagttttta ttcataaccg gttgatgcgg tttataaggc gctggatatg
     4741 aattaacgtc gtaactattt tagtgaaccg gaggggacaa tggctcaaga gcgttctgta
     4801 attaaattca tcaggccttg cctctccatt catgaagata agaaaatact tacatctgaa
     4861 tattgttggc agaacttgaa tgaagctctt gggtaaaata gagagaaaat acagcgacga
     4921 tgataccaac tgatgcaaga atgatatatc tatactccct gaaaaaatcc ttcactaaaa
     4981 tctccgctct gattcatcaa tcatcaaccc gatttgctat cttatccaga tatttttcca
     5041 tgttgtgttt gcgcaaataa ctattcacct ctgccgccca agattcacct ttaacgagaa
     5101 tagcccggct gtaacttgat gcgtacatgt aacgggtatc gttaagatta atcagcgttt
     5161 gatttacacc atcaacgcta caaccagaaa tctgactctg tcgctccagc gcccagtaat
     5221 acgcgccagg tgttctgggt tagcaggaaa atccctttca ttaagttcac taagtaccag
     5281 aaatgagggg acatgttgaa tacgtgaaat tttcggtgtc aaattgaaga ttagatccat
     5341 ataaaatcac aacggatgac cgttacaagc acctgctatc cgttgcaatg acctcttcat
     5401 ctgttaagat gcaggggcgt ttttgcatga ctcaatgatc accaataggt aaaaaatgtc
     5461 gacacttaaa cccgccgtct tctctttctt ttccggctca ggctttctag atcttggctt
     5521 cgaaaactcg ggctacgagg tggtttttgt gaatgaattt cacccaccgt ttatggatgc
     5581 ttacaagcac tcacgggcgg tgatgaacaa acctatccct cgttttggct atgcagaaga
     5641 gagcatcgag gaaattgatc acaagaaatt gaaagataac attaaagctc tcaagaaaga
     5701 aaaaagacta attggcttca tcggtggccc accatgccct gacttctccg ttgcaggtaa
     5761 gaacaaaggt cgcgacggag agaacggtaa gctgtctcaa agttacatcg atataattgt
     5821 tgaaatgaag cctgacttct tcttgtttga gaacgtcaaa ggtctttatc gcacactcaa
     5881 acatcgtgag ttttttgagc atctcaaaga acagttaatg gacgttggtt acgttcttca
     5941 tgaaaggttg ataaactcga ttgaatatgg cgccccgcaa gaccgtgaac gcattattct
     6001 gttgggtttc caaaataaaa atttaaccaa gtcctttaac tggagtggaa gattataccc
     6061 ggatcgtagt gcctttggat ataattggcc aactacgaat gaatttgttg aagactcaac
     6121 actggtaatg cctgagtcac ttcctgaaga gttaactgtt gactactggt ttaggaaaaa
     6181 caacgtttct gaacatgtga acgcaaagca tgtgtttact gcaagggccg ggttagttcg
     6241 ctttaaagct gtggctgaag gagacgatag caaaaaatct tacaaacgtc ttcatcgctg
     6301 gagatattct ccaacggcag cttacggtaa taatgaagta catttgcacc catataagcc
     6361 acgtagaata actgttgcag aagcacttgc cattcagtct ttaccgaaag aatttgaatt
     6421 gccatcttac atgactcttt caaatatgtt taaaactgtt ggtaacggtg ttccttacct
     6481 tgcagcattc ggaatagcaa aatccatatt agagcattta gggataaaag atgaatttaa
     6541 cagcaatgaa tcttgtgaat gcgatagcaa atcttcccaa aaacaggcag tttgattatg
     6601 tgaatgaaag taatgcaggg aagattgtga tcgactcgat taagttgcca gaaggtccaa
     6661 tacgctttaa gcgtttcaat ccaaccaaag gtgaaacgct cagagatgcc aaaattgata
     6721 gcatatctag ccaaatgcta tggcgtttgg ctaatgcaat tcaagaggat gcacctatca
     6781 atttagatag agtattcggt gcaagctata actctcgttc agttattgag agtttacttg
     6841 ctcatacgcc tcaattttat tggtgtaaac tcgaccgctt agaggttatt aacactaagc
     6901 agacaattaa aaaaggccac aagcatctta tatacttacc gaattcacca cacgaaaatg
     6961 gcaagctctc tgaatacaaa actaatattg taatatcaga gatgaacaca gaagttgtat
     7021 atcaaagtgt tgattcagaa acaatccgtc ccgttgaagg catgtcaatt gaagaatcaa
     7081 gaaggcatgc acaaatccag atagcattag taaaaatcgg ccatcatctg ggatacagaa
     7141 catgggttgc agctaatgac cgaggattaa aatacaatgg taaagcaatc gcccaaatgg
     7201 atggcgtcat tgacagtctg tcgaatgaaa aagtcctaag ttcctacagc gaagccattg
     7261 atggcgcaag attgatagat tgcatctggt ttagaaatgg taaattgatg cctgccgtga
     7321 tggagatcga gcatagtacc gggataataa gcggtttaac acgtatgaag aacttctgtg
     7381 attatgcccc tcgtctgcaa gatatacgat ggacaatcgt agccccagac gaatatcgag
     7441 aaaaagtaat tgagtttgca aatagaccac aatttcgtga gctagacaca aaattcttcc
     7501 cttactctgc cgtagaagag ttgtattctc tttgtgagcg tcgaaagcct caaggaataa
     7561 ccgataaatt cttagattct ttcatggaaa gatgcatcaa acattaaaaa agccggaatt
     7621 aaccaagttc ttatgactag tactgatata cgttgacact tttctgtctg taaaacgcct
     7681 gatgaacgtc ctcgggcgtt ttgtatttta gagctagaca tggcctctca tggttataaa
     7741 ttgttacgga ctcctttacc atatcgtggg tcggagcaag gtctgtagga cgcggaagta
     7801 aaaactcatt tttcaggatc ccgtttattc gatcagccag tgcattctgg tagcagtcat
     7861 gaccatcagt cattgaacag gttattccgt ttctctcatg gattgactga taaagcgcta
     7921 aacattattg cacccctctg tcagagtgac tgtaccagag aacctgtcgt ttttcttctc
     7981 ctcaatgcct gtctgaaggc ttttaccaca ttttctgtct gcagattttt cccaatatgg
     8041 taatcggcgc atatcaaggt agttgtctga gtcacaggca gttccctctt caccaggtca
     8101 ggaagtcagg gactctagtc aagtcggcca tatttacgca accagacaag aacggtgaag
     8161 cgcacctgaa tgctataccg ctgctgggcc tttttatagg tcatctcgcc tttttcaacc
     8221 tgctcaacaa cagctgtttt aaaggataga ggataatcac gttgggtgcg tttgaactca
     8281 gtcatcatca cgttctccgg atttaagtca gcaacgtgtc aacgctattc aggacgggtc
     8341 acaggcataa aaaaagacct cagttgaggt ctttttacat acttttggtg ccgaaggccg
     8401 gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct accgatttcg
     8461 ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt cgcacgccac
     8521 catgcaagcg ccagcttgct cgcacctcgc taagtgctga aattttcagc attgcatacc
     8581 gcgatgtgta acctttgtca cactccaggc accccgccct gccatgctct acacttccca
     8641 gacaacacca gagaaggacc aaaaaatgtc gatgataaaa agctatgccg caaaagaagc
     8701 gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac aagatattga
     8761 agtgcaggtg gattactgcg ggatctgcca ttccgatctg tcgatgatcg ataacgaatg
     8821 gggattttca caatatccgc tggttgccgg gcatgaggtg attggtcgcg tggtggcgct
     8881 cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga ttggctggac
     8941 ggcgcgtagc tgcgggcatt gcgacgcctg tattagcggt aatcagatca actgcgagca
     9001 aggtgcggtg ccaacaatta tgaatcgcgg tggctttgcc gagaagttgc gtgcagactg
     9061 gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc cgctgctgtg
     9121 tggtggtatc acggtcttta aaccactgtt gatgcaccat atcacagcca ccagccgcgt
     9181 tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc acgcaatggg
     9241 atgcgaggtg acagccttta gttcgaatcc ggcgaaagag caggaagtac tggcgatggg
     9301 tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagcactgg cggggcagtt
     9361 tgatctcatt atcaacaccg tcaacgtcag cctcgactgg cagccttatt ttgaggcgct
     9421 gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt ctgttccggc
     9481 ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca cgccttatga
     9541 gctgcgtaag ctgatgcgct ttgccgcccg cagcaaggtt gcaccgacca ccgaattgtt
     9601 cccgatgtcg aaaattaacg acgccatcaa gcatgtgcgc gacggtaagg cgcgttaccg
     9661 cgtggttctg aaagccgact tctgatccct ttgccgggtg cctgtcgccc ggcactaaaa
     9721 tctctttgga aggcatctcg caatctgctg aatttagcgg tgaagagtgc gcagccgttg
     9781 cctatactcc aggcaggaaa ctggaggaaa tctcatgagc gaacccctgt taattgcccg
     9841 cacgccagac acagaactgt ttttactgcc gggaatggct aaccgtcacg ggctgattac
     9901 tggcgcaacg gggacgggta aaaccgttac gctgcaaaaa ctggcagagt cattgtcgga
     9961 aatcggcgtg ccggtgttta tggctgatgt gaaaggcgat ctgaccggta tcgcgcaggc
    10021 aggaacggca tcggaaaaac tactcgcaag gcttaaaaat atcggcgtca atgactggca
    10081 accgcatgct aatccggtgg tggtgtggga tatctttggc gagaaaggcc atccggtgcg
    10141 ggcgacggtt tcggatctgg ggccgctgtt gctggcgcgg ctgttgaatc tcaacgatgt
    10201 gcaatctggc gtgctgaata tcatcttccg tattgctgac gatcagggat tgttgctgct
    10261 cgactttaaa gatctgcggg cgattaccca gtacatcggc gataacgcca aatctttcca
    10321 gaatcagtac ggtaatatca gtagcgcatc ggttggtgcc atccagcgcg gactgttgtc
    10381 gctggaacag caaggcgcag cacacttctt tggcgagccg atgctggata tcaaagactg
    10441 gatgcgcacc gataccaacg gtaaaggcgt tatcaatatc ctcagcgccg agaagcttta
    10501 tcagatgccg aaactgtacg ccgccagcct gctgtggatg ctttcagagt tgtatgaaca
    10561 attgccggaa gcaggcgatc tggagaaacc aaaactggtg tttttcttcg acgaggcaca
    10621 tctgctgttt aacgatgcac cgcaggtact gctggataag attgagcagg tgatacggct
    10681 tattcgctca aaaggcgtag gcgtctggtt cgtttcgcaa aacccgtctg atattccgga
    10741 taatgtgctc gggcagctcg gtaatcgcgt tcaacacgct ttgcgtgctt ttacgcccaa
    10801 agatcagaaa gcggtaaaag ctgcggcgca aaccatgcgg gccaatccgg catttgatac
    10861 cgaaaaggca atccaggaac tggggaccgg cgaggcgtta atctcgtttc ttgatgtgaa
    10921 aggaagtcct tcagtggtgg aacgggcgat ggtgattgcg ccttgttcgc ggatgggacc
    10981 ggtgacggaa gatgagcgta atggcttgat taatcactct ccggtgtatg gcaagtacga
    11041 ggatgaggtg gaccgggaat ccgcctatga gatgttgcaa aaaggcgtac aggcgagtat
    11101 tgaacagcaa aataatcctc cagcgaaagg gaaagagata gcggtagatg acggcattct
    11161 tggtggattg aaggatattt tgttcggcac taccggacca cgcggcggga agaaagatgg
    11221 tgtggtgcaa acaatggcca aaagcgccgc tcgccaggtg acgaatcaga ttgtgcgtgg
    11281 aatgttgggg agtttgctgg gggggagaag aaggtaagtc aggcgcgatc tgtccccctc
    11341 accctaaccc tctccccaaa ggggcgagag gaccgatcga actcgtattt gactttatca
    11401 acaatgtgaa agggagcttt cgctcccttg cttggttacg attttctcat taacagccac
    11461 aggctgatta agaagaagct ggcgcttggc aacagtgcgc cgatgatcgg cgggatgcca
    11521 taaaccaacg tcaacgggcc gaagatctgg tccagtacgt agaagacaaa accgaagctg
    11581 ataccggtga ccacacgcac gcccatcggt acgctacgca gtgggccaaa gatgaacgac
    11641 agcgccatca gcatcatcac cgccacggat agcggctgga agattttgct ccacatgttg
    11701 agctgataac gtccagcgtc ctgaccgctc gacttcagat acttcacgta gttgtgcaaa
    11761 ccgctgattg agagtgcatc cgggtccagc gccaccacgc ccagtttgtc tggcgtgagg
    11821 ttggttttcc aggtgccgct caccgtctgc gaaccggtaa tctgtttcgg attggtcaga
    11881 tcagattcat caacctgcga cagacgccag actttatgtt ccgggtcaaa cttcgcagta
    11941 gcggcatagc gtacggattg cagacgacga ttctcgttaa aggcataaat gctgatgcca
    12001 cctaactctt cgtcaccttt aacccgctca atgtagacga agttgttgcc atctttcgcc
    12061 cataagcctt gctgggtaga gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta
    12121 cgcgccatct gctcgccctg cggcgcgacc cattcgccaa tcgccatcgt cagcaagacc
    12181 agcggaatgg cggttttcat caccgacagc gccacctgca tacgggtaaa accagaagcc
    12241 tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc
    12301 agagccgcca tcgggaagaa gatttgcaca tctttcggca cgctcagcaa ggtatacatt
    12361 cctgcgccta acgcgtcgta actcccctgc ccggcttttt tcagctgatc gacaaacttg
    12421 ataatgcccg acagcgacac cagcatgaac agcgtcatca tgatggtggt gaaaatagtt
    12481 ttaccgatat agcggtcaag tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa
    12541 aactggcgcg caggcggcgg accggcacgg tgtcccaaag gttgagaaca atcgccaaag
    12601 ccagataaat caggttaacg gtccacatcc acagcgtcgg gtccagctta cctttaccgc
    12661 cgttcgattt cagggaggtc tggatcagga agaacaacag atacagcagc atggctggca
    12721 gcatcgatag tacgcgcccc tgacgcgggt taaccacgct cagcggtacg accataagtg
    12781 ccatcataaa cacggtgaac accaacgtga tacgccagtt cagttctgcg cgagcacgat
    12841 cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc ggtatcgttc gggtcgagcg
    12901 ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa atccgtaatg cggaaatcac
    12961 gtaacagtgc agtgccttcg aagcgcgttc cctggttgag agtgacgacc tgggagccgt
    13021 cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt gcattacctt
    13081 ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct gccgtcaacg ctttcgatga
    13141 acagcaccga actgccatta gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc
    13201 cagggttcgc tttcgcttct gctaacactt catcctgatg acgcgatgac cacggtcccg
    13261 cccacatcac gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc attgccgctt
    13321 tcaccagaac cgctttgctc aggccgcagg catgcattac cgtaatttca ctttcggtat
    13381 acagtttgcc cagcgtcatc agcagcccga ggaacaggct taatggcagg ataagctgcg
    13441 ccatttccgg cacgcccaac ccgagaaggg agagcaccag attcgccgga atatcgccgt
    13501 caaccgctgc gccgaggatc ctcactaact tttgacagaa gaagatcaaa agcaagatga
    13561 agagtatcgc cagctggctt ttgagcgtct cccgcaccag atatcttatg attatcactt
    13621 taaatacgcc cgtaaaaact cgtcttttgc aggattttag cttgtttcat ggcttaaacg
    13681 tcatttattc tcttgagtcg tcgaaatcgt cgctaagata attatactca acggattcac
    13741 ctctcagatt ttgttctgac gtgccaatgc cgtaataacg ttaagattaa cacgaagtca
    13801 tcgcaacagc ggacatgagt tacgaaagct tgcaattcta tctgtagcca ccgccgttgt
    13861 ctttaagatt caggagcgta gtgcatggag tttagtgtaa aaagcggtag cccggagaaa
    13921 cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca
    13981 gaacagctcg ataaaatcag cgatgggtac atcagcgccc tgctacgtcg gggcgaactg
    14041 gaaggaaaac cggggcagac attgttgctg caccatgttc cgaatgtact ttccgagcga
    14101 attctcctta ttggttgcgg caaagaacgt gagctggatg agcgtcagta caagcaggtt
    14161 attcagaaaa ccattaatac gctgaatgat actggctcaa tggaagcggt ctgctttctg
    14221 actgaactgc acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc tgtcgagacg
    14281 gcaaaagaga cgctctacag tttcgatcag ctgaaaacga acaagagcga accgcgtcgt
    14341 ccgctgcgta aaatggtgtt caacgtgccg acccgccgtg aactgaccag cggtgagcgc
    14401 gcgatccagc acggtctggc gattgccgcc gggattaaag cagcaaaaga tctcggcaat
    14461 atgccgccga atatctgtaa cgccgcttac ctcgcctctc aggcgcgcca actggctgac
    14521 agctacagca agaatgtcat tacccgcgtt atcggcgaac aacagatgaa agagctgggg
    14581 atgcattcct atctggcggt cggtcagggt tcgcagaacg aatcgctgat gtcggtgatt
    14641 gagtacaaag gcaacgcgtc ggaagatgca cgcccaatcg tgctggtggg taaaggttta
    14701 accttcgact ccggcggtat ctcgatcaag ccttcagaag gcatggatga gatgaagtac
    14761 gatatgtgcg gtgcggcagc ggtttacggc gtgatgcgga tggtcgcgga gctacaactg
    14821 ccgattaacg ttatcggcgt gctggctggc tgcgaaaaca tgcctggcgg gcgtgcctat
    14881 cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg ttgaagtgct gaacaccgac
    14941 gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg ttgagcgttt tgagccggaa
    15001 gcggtgattg acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg tcatcacatt
    15061 actggtctga tggcgaacca caatccgctg gcccatgaac tgattgccgc gtctgaacaa
    15121 tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca actggagtcc
    15181 aattttgccg atatggcgaa cattggcggt cgtcctggtg gggcgattac cgcaggttgc
    15241 ttcctgtcac gctttacccg taagtacaac tgggcgcacc tggatatcgc cggtaccgcc
    15301 tggcgttctg gtaaagcgaa aggcgcaacc ggtcgtccgg tagcgttgct ggcacagttc
    15361 ctgttaaacc gcgccgggtt taacggcgaa gagtaagttt gcgttttacc ctcaccctaa
    15421 ccctctccct aagggagagg gaaccgatcg agcgcaatag aaattcattg ctcatctttt
    15481 agggtgagga atgcagcaat atttaaatcc accacaagaa gccccattta tgaaaaacgc
    15541 gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcagtt
    15601 ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca tcgcctgtga
    15661 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
    15721 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
    15781 gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
    15841 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
    15901 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
    15961 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
    16021 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc
    16081 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
    16141 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
    16201 gtcggtactg accacgcagg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
    16261 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
    16321 tggaaagcgg aatccggcgg caccattacc cgtcagatgc gccgtctcgg caactccgtg
    16381 gactgggagc gtgaacgctt caccatggac gaaggcctgt ccaatgcggt gaaagaagtt
    16441 ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt aaactgggat
    16501 ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
    16561 atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa ccgcagatgg taaagattat
    16621 ctggtggttg cgactactcg tccggaaacc ctgctgggcg ataccggcgt ggccgttaac
    16681 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
    16741 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
    16801 aagatcaccc cggcgcacga ctttaacgac tatgaagtcg gtaaacgtca cgccctgccg
    16861 atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gtgcccaggt gttcgatacc
    16921 aaaggtaacg aatctgacgt ttattccagc gagatccccg cagaattcca gaaactggag
    16981 cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
    17041 attaaaccac atgacctgac cgttccttat ggcgaccgtg gtggcgtggt catcgaaccg
    17101 atgctgaccg accagtggta cgtgcgtgcc gatgtcctgg cgaaaccggc agttgaagcg
    17161 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
    17221 atgcgcgata ttcaggactg gtgtatctct cgtcagttgt ggtggggtca ccgtatcccg
    17281 gcatggtatg acgaagcggg taacgtttat gttggccgca acgaagacga agtgcgtaaa
    17341 gaaaataacc tcggtgctga tgttgtcctg cgtcaggacg aagacgttct cgacacctgg
    17401 ttctcttctg cgctgtggac cttctctacc cttggctggc cggaaaatac cgacgccctg
    17461 cgtcagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt cttctggatt
    17521 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
    17581 ccgttccaca ctgtttacat gaccggcctg attcgtgacg acgaaggcca gaagatgtcc
    17641 aaatccaagg gtaacgttat tgacccactg gatatggttg acggtatttc gctgccagaa
    17701 ctgctggaaa aacgcaccgg caatatgatg cagccacagc tggcggacaa aatccgtaag
    17761 cgcaccgaga agcagttccc gaacggtatt gaaccgcacg gtactgacgc gctgcgcttc
    17821 accctggcgg cgctggcttc taccggtcgc gatatcaact gggatatgaa acgcctggaa
    17881 ggttaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacaca
    17941 gaaggtcagg attgcggctt caacggcggc gaaatgacgc tgtcgctggc ggatcgctgg
    18001 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
    18061 ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga accagttctg tgactggtat
    18121 ctggaactga ctaagccggt aatgaacggt ggcaccgaag ccgaactgcg cggtactcgc
    18181 catacgctgg tgaccgtgct ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
    18241 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
    18301 atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac
    18361 acagagtggc tgaagcaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc
    18421 gcgccgggca aaccgctgga gctgctactg cgtggttgca gcgcggatgc agaacgtcgc
    18481 gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc tggaaagcat caccgtgctg
    18541 cctgccgatg acaaaggtcc ggtttccgtt actaagatca tcgacggcgc agagctgctg
    18601 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg
    18661 gcgaagatcg aaggcgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc
    18721 gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaaa agctggaagg ctatgcagaa
    18781 gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt
    18841 aatgaaaacg aaggccggag catgctccgg ccttttttat ctcttacaga aatggtaaag
    18901 aaggcataat tccccgtgaa atcctggcta agaagccttt atctggttgt tcttctttat
    18961 tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa
    19021 tcatccatat tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca
    19081 acgcacacat gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc
    19141 catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg
    19201 tgctaacagc aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt
    19261 ccataaattg ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat
    19321 ttaatatttg atcgatgata atataacaag caacagcgag aaaaggtaat aaagctaaaa
    19381 atgcgagaat ggcatatttt atacaatacg tagtatctaa tttcaccttg aaacgatgta
    19441 taccgaaact ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa
    19501 aaataccaat ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac
    19561 ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg
    19621 ccattaaaat cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac
    19681 taaatcttac gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca
    19741 taaatataag caaaaccaac agtaacaaag tcaaaacaca gccaacaagt ggcatatctg
    19801 ctgacactgt cattaagatt gcgaaatagc aaaaaacaaa aacaagacaa ctaacaaaaa
    19861 cattcccacc agtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga
    19921 gataacgctt acatttcatt aatgcccatg gcaaatagat acctagcgtt ataatcgtta
    19981 acaacacatt caccacacaa ataagaaaat atctctcacc tgtaccat
//
LOCUS       DAPPNJ010000075        19983 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000075 DAPPNJ010000000
VERSION     DAPPNJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.075"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            53..439
                     /locus_tag="RPM73_002221"
     CDS             53..439
                     /locus_tag="RPM73_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDX6520575.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLANLTGYSDKPLDAIGAVIVSALAVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            426..707
                     /locus_tag="RPM73_002222"
     CDS             426..707
                     /locus_tag="RPM73_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDX6520576.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            707..1321
                     /locus_tag="RPM73_002223"
     CDS             707..1321
                     /locus_tag="RPM73_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDX6520577.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAPADQAMFIA
                     QVGHESMGFSAVVENLNYTPSALVATFGKRITQQQADALGRTSGHAARQDAIANLVYS
                     NRLGNKAPGDGWKYRGRGLIQITGLHNYRICGAALKLDLVTSPEQLEQELQAARSAAW
                     FYTSKGCMIYGADINRVTRIINGGLNGIEDRKVRYNKARAALLV"
     gene            1318..1710
                     /locus_tag="RPM73_002224"
     CDS             1318..1710
                     /locus_tag="RPM73_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDX6520578.1"
                     /translation="MKMSYWALILTFIACVAGGLVWSANHYHGKFLDEQKRADAAEQR
                     ADSTEAITANVLRTMAITNIIQEANQHAKQQIALESQRTQEDIKVAVADDDCASRPVP
                     AAAADRLRKYANSLRPGSGSSVTSQPDG"
     gene            1957..2277
                     /locus_tag="RPM73_002225"
     CDS             1957..2277
                     /locus_tag="RPM73_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="negative regulator GrlR"
                     /protein_id="HDX6520579.1"
                     /translation="MKDGIYFVVFRSNQRDFGNGTVVVKNNAVNGGDFGFTYQGKIDG
                     SQLILRVSQHDLNVTSVFPGVKNFELSLSLQERGRDYLLNGSVVGMPQMQISISAKYI
                     GDLI"
     gene            2370..2720
                     /locus_tag="RPM73_002226"
     CDS             2370..2720
                     /locus_tag="RPM73_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6520580.1"
                     /translation="MPPRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            2846..3340
                     /locus_tag="RPM73_002227"
     CDS             2846..3340
                     /locus_tag="RPM73_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDX6520581.1"
                     /translation="MAGTAGRSGRRPKPTARKALSGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            3574..5070
                     /locus_tag="RPM73_002228"
     CDS             3574..5070
                     /locus_tag="RPM73_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDX6520582.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGSRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLLSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKTIPGND
                     DVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            5082..5264
                     /locus_tag="RPM73_002229"
     CDS             5082..5264
                     /locus_tag="RPM73_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520583.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            5264..6505
                     /locus_tag="RPM73_002230"
     CDS             5264..6505
                     /locus_tag="RPM73_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDX6520584.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGNLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            6483..7133
                     /locus_tag="RPM73_002231"
     CDS             6483..7133
                     /locus_tag="RPM73_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDX6520585.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            7148..8353
                     /locus_tag="RPM73_002232"
     CDS             7148..8353
                     /locus_tag="RPM73_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDX6520586.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAEA"
     gene            8403..8603
                     /locus_tag="RPM73_002233"
     CDS             8403..8603
                     /locus_tag="RPM73_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520587.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            8606..8929
                     /locus_tag="RPM73_002234"
     CDS             8606..8929
                     /locus_tag="RPM73_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDX6520588.1"
                     /translation="MLLTMEEIKAQLRLDEDFDTDDRHLQLLACAAQKRTETYLNRTL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            8926..9336
                     /locus_tag="RPM73_002235"
     CDS             8926..9336
                     /locus_tag="RPM73_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDX6520589.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADTFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGEFTQSHGGSNGDSLFSR"
     gene            9311..9817
                     /locus_tag="RPM73_002236"
     CDS             9311..9817
                     /locus_tag="RPM73_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520590.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFITIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQHNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            9814..10374
                     /locus_tag="RPM73_002237"
     CDS             9814..10374
                     /locus_tag="RPM73_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520591.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEIPLPS"
     gene            10383..10553
                     /locus_tag="RPM73_002238"
     CDS             10383..10553
                     /locus_tag="RPM73_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDX6520592.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            10537..12033
                     /locus_tag="RPM73_002239"
     CDS             10537..12033
                     /locus_tag="RPM73_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDX6520593.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTMIASSIQDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            12033..12389
                     /locus_tag="RPM73_002240"
     CDS             12033..12389
                     /locus_tag="RPM73_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDX6520594.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            12389..12658
                     /locus_tag="RPM73_002241"
     CDS             12389..12658
                     /locus_tag="RPM73_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDX6520595.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQNSVDQMSPADLNAAAWLVAGFFLQA"
     gene            12800..14635
                     /locus_tag="RPM73_002242"
     CDS             12800..14635
                     /locus_tag="RPM73_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX6520596.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            14696..16024
                     /locus_tag="RPM73_002243"
     CDS             14696..16024
                     /locus_tag="RPM73_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HDX6520597.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            16021..17100
                     /locus_tag="RPM73_002244"
     CDS             16021..17100
                     /locus_tag="RPM73_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDX6520598.1"
                     /translation="MNDNVTLRVDGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGMLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            17100..17648
                     /locus_tag="RPM73_002245"
     CDS             17100..17648
                     /locus_tag="RPM73_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HDX6520599.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            17648..18073
                     /locus_tag="RPM73_002246"
     CDS             17648..18073
                     /locus_tag="RPM73_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDX6520600.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVLTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            18060..19118
                     /locus_tag="RPM73_002247"
     CDS             18060..19118
                     /locus_tag="RPM73_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDX6520601.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            19109..19693
                     /locus_tag="RPM73_002248"
     CDS             19109..19693
                     /locus_tag="RPM73_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDX6520602.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPTGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDANITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            19697..>19983
                     /locus_tag="RPM73_002249"
     CDS             19697..>19983
                     /locus_tag="RPM73_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520603.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALE"
BASE COUNT         4619 a         4986 c         5967 g         4411 t
ORIGIN      
        1 tttttgtttc ccctcgttct gagaggacca acagcaatta agagggggct aaatgtccga
       61 tccgatttcc ggtactgggc tggctggtgg tgccctgacg ggtgccagtg tttatggact
      121 gctgaccgga actgattacg gcgttgtatt tggcgcattt gcaggggctg tattctacat
      181 agcaacagct gcagatctga gtgcatcgcg ccgactggca tattttatcg tgtcatatat
      241 tgccgggatt ctttgctctg ggttggttgg ctccaagctg gcgaacttga ccggatacag
      301 tgataaacct ctggatgcta ttggtgccgt aatcgtctct gctttagccg ttaaaatcct
      361 gacgttcctg aataatcagg atatcggctc gctggtggcg ctcataacgc gccggggagg
      421 ttcaggtgga gctaaatgac ccgacagcaa ctataaatgc gctgttatgt gcttgtgttg
      481 ttattactct gatgttttat cgtcgtggtg attcgcggca tcgtccttgg gtttcacgtt
      541 tagcctggct gattactgtt acatacagtg ctgttccgtt ggcctatctc tgtgggattt
      601 atccccattc ctcatggccc attatcgtgg cgaacactat ttttctttcc gtgctggtgg
      661 ctgtcagagg caacgttgca cgtctggttg atcatctgag gcactaatga accaacaatt
      721 atttcaaaag gcggctggta ttagcgccgg gctggctgcg cgctggtttc cgcacattga
      781 tgcggcgatg aaggaattcg gcattacagc accagcggat caggcaatgt ttatcgctca
      841 ggtaggccat gagtcgatgg ggtttagcgc cgtagttgaa aatcttaact acacaccatc
      901 tgcgcttgtg gcgacgttcg gaaagaggat cacacagcag caggctgatg cccttggcag
      961 aacatccgga catgcagctc gtcaggatgc tattgccaat ctggtgtata gcaaccgact
     1021 gggtaacaaa gcaccaggtg atggctggaa atatcgtggt agaggattaa ttcaaatcac
     1081 tggcctccat aattatcgca tctgtggcgc ggcgctgaag ttagatctgg tgacttcacc
     1141 tgaacaactg gaacaggaac tacaggctgc gcgctcagct gcatggttct acacctctaa
     1201 aggttgcatg atctacggtg ccgatattaa ccgtgttacg cgcatcatta acggcggttt
     1261 gaacggtatt gaggatcgta aggtccgata caacaaggcg cgggcggcgc tgctggtatg
     1321 aagatgagtt attgggcgct cattttaacg tttattgctt gtgtcgctgg tggtcttgtc
     1381 tggtcagcga atcactatca tggaaagttt ctggatgagc agaagcgtgc tgatgctgcg
     1441 gaacagcgag ctgattctac tgaggctatc accgcgaatg ttctgcgtac tatggcaata
     1501 acgaacatca ttcaggaggc gaatcaacat gcaaaacagc agatcgcact ggagtcacag
     1561 agaacccagg aagatatcaa agtggctgtt gcggatgatg attgtgcttc acgtcctgtg
     1621 cctgctgccg ctgctgaccg gttgcggaag tacgcgaaca gtttacgtcc aggttccggt
     1681 agttccgtta ccagccagcc tgacggctga aacccctcag cctgatttac ctgatcattt
     1741 tacgtggggc tcgagcttag atctgaatgt cgccttgttg tctgcattgg cgcagtgtaa
     1801 taccgataaa gctgacatca gaaggattga agttgagcgt ggtcacatca tgcaaaaaaa
     1861 atgatgttaa ctttgttttg ttccttgatt tgatatgtga tggcccaata gatacaaagc
     1921 acctgatttt ggtgactctt ttaaagggct ttacacatga aagatggtat ctattttgtt
     1981 gttttcagaa gcaatcaacg tgattttggt aatggtaccg tagttgtcaa aaacaatgca
     2041 gtaaacggcg gagattttgg ttttacgtat cagggaaaaa ttgacggtag ccaacttatt
     2101 ctgcgcgtat cgcagcatga tttaaatgtc acctcggttt tccctggggt aaagaacttt
     2161 gaattgagtc tttctttgca ggaacgagga cgtgattacc tgttaaatgg atctgtggtc
     2221 ggaatgcctc agatgcaaat ttcaattagt gcaaaataca ttggtgatct gatttagttt
     2281 atcgagatga taattgaacc gcctccgggc ggttttttat tgccatttct atggtctgtt
     2341 ccatcgtaat aacttaaagg gaagcattaa tgccgccacg aaccccgaaa gcctgccgtg
     2401 ttcgcggctg ccgccatacc actactgacc cttcaggcta ctgcgaaagc cacaaaagcg
     2461 aaggctggaa gcaatacaaa cctggacaat cccgtcatca gcgcggctac ggttcgaagt
     2521 gggacagtat ccgcgcgcgt gttctgaagc gtgacaaagg cctgtgtcaa ttatgtctgc
     2581 gtgctggtgt ggtgcgtgag gcgaaaactg ttgaccacat catccctaaa gcgcatggcg
     2641 gcactgatgc tgacagtaat ctgcagagtc tgtgctggcc gtgtcataag gcgaagacgg
     2701 cccgtgaacg gttaaagtga taataattct caactgtctg aggggagggg cgggtcaaat
     2761 ccctgcagcc tgacgtcttc cggactgccc gccccatcgt ttttttatac ccgcgaaaaa
     2821 tgaaatttaa ccaggagtgc cgcatatggc tggaacggcg gggcgttccg ggcgtcgccc
     2881 caagccaacg gcgcgcaagg cgctgtccgg aaaccccggc aagcgagccc tgaacaaaga
     2941 tgaacctgtt tttacgccca tcaaaggtgt tgagccaccg gagtggttcg ctgaagagga
     3001 tctccctctc gccacgatca tgtggcaact gacaaccaaa gaactctgcg gtcagggcct
     3061 gctgtgcgtg actgacctag cggtacttga gcggtggtgc gtggcctatg agttctggcg
     3121 acgtgccgtg aaaaatattg ccagacaggg caacaccatc accggtgcaa tgggcggtat
     3181 ggtcaaaaat ccggagctga ccgctaagaa agaacaggag tccgagatga gcagcacggg
     3241 ggcaatgctc ggactcgacc ccagcagccg ccagcgtctg attggcctgg cggggcagaa
     3301 gaaagccact aacccgtttc tgaaaatcat cgaatcatga gccggaaatc ttaccccaac
     3361 gtaaatgctg ccaatcagta tgcccgtgat gtcgtgcgcg gaaagattgt ggcctgccag
     3421 tttgtgattc aggcctgcca gcgccatctt gatgacctga tggcggaaaa aaataagtcg
     3481 tttcgttacc gcttcgacaa ggacctggct gaacgggccg cgaaatttat tcagctgttg
     3541 ccacacacca agggggagtg ggcattcaag aggatgccca tcacgctgga accgtggcag
     3601 ctctttgtga tctgctgtgc gtttggctgg gtcaataaag gctcccggct gcgccgcttc
     3661 agggaggtgt ataccgaaat cccccgtaag aacggcaaat cggcaatctc tgccggtgtt
     3721 gccctgtatt gttttgcctg tgataacgag tttggcgcgg aagtgtattc cggtgccacg
     3781 acagagaaac aggcgtggga agtctttcgc ccggcgcgac tgatgtgtaa acgcacaccc
     3841 atgctgacgg aagcgttcgg gattgaggtt aacgcctcaa acatgaaccg tccggaggat
     3901 ggcgcgcggt ttgaaccgct gatcggtaac cccggtgatg gttcatcacc ccactgtgcc
     3961 gtggtggatg aatatcacga gcacgccacc gatgcgcttt ataccacgat gcttaccggg
     4021 atgggcgcgc gacgtcagcc actgatgtgg gccatcacca ccgccgggta caacattgag
     4081 gggccgtgct acgacaagcg gcgggaagtt atcgagatgc tcaacggttc ggtacccaac
     4141 gatgaattgt tcgggatcat ctataccgtt gacgaaggcg atgactggac cgacccgcag
     4201 gtgctggaaa aagctaaccc gaatattggc gtgtcggttt atcgcgaatt tttgttaagt
     4261 cagcagcagc gtgcgaaaaa taacgcccgt ctggcaaacg tctttaaaac aaaacacctc
     4321 aatatctggg tgtcggcgcg ttcggcgtat ttcaacctgt tgagctggca gagctgcgag
     4381 gataaatcac tgacccttga gcagttcgag gggcaaccgt gcattctggc ctttgacctg
     4441 gcgcgtaagc tggatatgaa cagcatggcg cgactttata cccgcgagat tgacggtaaa
     4501 actcattact acagtgtggc cccgcgtttc tgggtaccgt atgacacggt gtacagcgtc
     4561 gagaaaaatg aagatcgacg gacagccgaa cgctttcaga aatgggtgga aatgggcgtt
     4621 ctgaccgtta ccgatggtgc ggaggtggat tatcgctaca tcctcgaaga ggccaaagcg
     4681 gcgaacaaaa tcagcccggt cagtgagtca cccattgacc ccttcggggc gaccgggttg
     4741 tcacatgacc ttgctgatga agacctgaac cccatcacta tcattcagaa ctacaccaac
     4801 atgtccgacc cgatgaaaga gctggaagcg gcaattgaat cggggcgctt tcatcatgat
     4861 ggcaatccca tcatgacctg gtgtatcggc aacgtggtcg gcaaaaccat tccgggtaac
     4921 gatgatgtgg tgaagcccgt caaagagcag gcggaaaaca aaatcgatgg tgcagttgcg
     4981 ctgattatgg cggttggcag agccatgctg tacgagaaag aagacacgct gtctgaccac
     5041 attgagtcct atgggatccg ctcgctttaa ctgaggtaat tatgatcatg ctgattctcg
     5101 cgcctctggt gggcgtgctg ggggcgcttt tgctggcgta tggtgcctgg ctgatttatc
     5161 ccccggcggg gtttgttgtt gccggggcgt tgtgcctgtt ctggtcgtgg ctggtggcgc
     5221 gatatctcga ccgtacacag tcgtctgtcg gcggaggtaa atagtgttct tttcgggatt
     5281 atttcaacga aaaagtgacg caccggtgac cacgccagca gagctggcgg acgctatcgg
     5341 gctgtcctac gacacctata ccggaaagca gatcagcagt cagcgggcca tgcgactgac
     5401 ggcggttttt tcctgcgtca gggtgctggc agagtcggtc gggatgttgc cctgcaacct
     5461 gtatcacctg aacggcaacc tgaaacagag agccaccggc gaacgtctgc ataagctgat
     5521 ttccacgcat cccaatggct atatgacgcc gcaggagttt tgggagctgg tggtcacctg
     5581 tctgtgcctg cggggaaact tttacgccta caaagtgaaa gcatttggcg aagtggctga
     5641 actgctgccc gtcgatcccg gctgtgtggt accgaagctt aacagtagct gggagccggt
     5701 ctatcaggtc acattcccgg acggctccac ggatgtgctg agccaggaag atatctggca
     5761 tgtgcgcaca ctgacgctgg acggactggt ggggctgaat cccatcgcct atgcccgcga
     5821 ggcaatatcg ctggcggcag cgaccgaaga gcacggggcc agactgttca gcaatggtgc
     5881 ggtgacgtcg ggtgtgttgc gtacagagca gacgctgtca gatcaggctt acgagcgcct
     5941 gaagaaagat tttgaggagc gtcacaccgg gcttggcaat gctcaccgcc cgatgatcct
     6001 tgagatgggg ctggactgga agtcgatggc gctgaacgcc gaggacagcc agttcctgga
     6061 aacccgcaag tttcagcttg aagaaatctg tcgtctgttc cgggtgccat tgcacatggt
     6121 gcagaacacc gatcgcgcca ccttcaacaa tatcgaagag ctggggctgg gatttatcaa
     6181 ctattcactg gtgccgtatc tgacccgcat tgagcagcgg atcaacaccg gactggtacg
     6241 aaaaagtaag cagggcgttt attacgccaa atttaacgcc ggggccttac tgcgcgggga
     6301 tatgaagtcc cgttttgaag cctacgctac cgggatcaat tggggaattt actctcccaa
     6361 tgactgccgc gacctggaag atatgaatcc gcgtcccggt ggtgatgtct atctcacacc
     6421 gatgaacatg accacgaaac cctccgatgg cagtaaggcc ggtaagcaga aggataacgc
     6481 caatgcagac gaaacaacgt cttgatgtac cgctgagtct gaaatctgtc agtgactccg
     6541 gtgagtttga agggtatggc tccgtctttg gtgtaaagga cagccacgat gatgtggtga
     6601 tgtccggggc atttgctgct tccctgcggg cgtggagtga cagaaaagcg ttacctgcgc
     6661 tgctctggca gcaccgcatg gatgaaccca tcggtgttta caccgaaatg aaggaagacg
     6721 atgtcgggct ttacgtcagg ggacggttgc ttattgatga tgatcccctc gcaaaacgcg
     6781 cacatgcaca catgaaggcc ggttcgttaa ctggcctttc tattgggtac gtcctgaaag
     6841 actgggaata cgaccggacg aaagaagcct ttctgctgaa agaaatcgac ctctgggaag
     6901 tcagtctggt gacgttcccg tctaacgacg aggcgcggat cagcgacgtc aagaacgcac
     6961 tggcccgcgg tgaaatcccc gaacagaaaa aaatcgaaag agtcctgcgt gatgtcggac
     7021 tctcccgtac ccaggccaaa gcattcatgg ccgggggcta tggcgcactg tccctgcgcg
     7081 acgctgagga tgtgggctct gcactgaatg cactgaaaaa tctgaacttc taatcaggag
     7141 aaatacgatg gcggttgata ttaaagatgt ggaacaggtc gcgcaggagc tgcagcagaa
     7201 gtttgacgac ttcaaggcaa agaacgacaa gcgcgtggat gcgattgagc aggaaaaagg
     7261 caaacttgcc ggacaggtgg aaaccctgaa cgggaaactc agcgagctgg aaaatctcaa
     7321 aagcgacctt gaaaaagagc tgcttgagct gaaacgtccg gcaggtggtg cgcaaaataa
     7381 actggccaca gagcacaaag aggcgtttgt gggcttcctg cgtaaaggcc gtgaagacgg
     7441 tctgcgcgat ctggagcgca aggcattgca ggtgggcacc gatgaagacg gtggctatgc
     7501 cgtgccggaa gcactggatc gcaacattct caccctgctg aaagatgaag tggtgatgcg
     7561 ccaggaagcc acggtgatca ccgttggcgg ttccgactac aaaaaactgg tgaatctggg
     7621 cggcacggct tccggatggg ttggcgagac tgatgcacgc tcccagaccg ccacctcaaa
     7681 actgggactg attgaacctt tcatggggga aatctacggc aacccgcagg ctacccagaa
     7741 aatgctggac gatgccttct tcaacgtgga ggcctggatc aacagcgagc tggcaaccga
     7801 atttgccgaa caggaagaaa ttgcctttac ctcaggcgat ggcaccaaga agccgaaagg
     7861 gttcctggcg tatgaatcca ctgatgaaac cgataaggtc cgggcgttcg gcaaacttca
     7921 gcatattgta tccggcgaag cgactgcggt gaccgcagac gccattatca aactgattta
     7981 cacgctgcgt aaggcacacc gtactggcgc gaagttcatg atgaacaaca acagcctgtt
     8041 tgccatccgt ctgctgaaag acagcgaggg taactatctg tggcgtccgg ggctggagct
     8101 ggggcagccg tcctctctgg cgggttacgg tatcgctgaa aacgaacaga tgccggatat
     8161 cgcagctgat gcgaaagcca ttgcatttgg taacttcaaa cggggttaca ccatcgttga
     8221 ccgtatcggc acccgcattc tgcgtgaccc gtacaccaat aaaccgtttg tcggttttta
     8281 taccaccaag cgcaccggcg ggatgctggt cgattcgcag gccatcaaac tgctgaagat
     8341 tgcagaggcg taatcattca tgggcgcgga accgcgcccc ctgttctgac gggtgaagaa
     8401 tcatgatcct gaaacaagat ctgaaatggt caccggacgg tatgcgtgtt gaggtcattc
     8461 gggccggtga gtatgacgac ggggcgcttc ctgcccgtgt gcaggagatt gcacttcagg
     8521 ccgggttagc agagcgcgga atcagtgcaa aaagcagtaa agcggcaaaa gagaaaaaag
     8581 ccacgaccag taaagagggc tgagcatgct tctgacaatg gaagagatta aagcccaact
     8641 ccggctggat gaggatttcg atactgatga ccgccatctg caactgctgg cctgtgcggc
     8701 acaaaagcgg acggaaacgt atctgaaccg gacgctctat gcaccggatg aaaccattcc
     8761 ggacagcgat ccggacggtc tgcacctgcc ggatgatatt cgtctgggga tgctgatgct
     8821 tatcagccat ttttacgaaa accgctcgtc ggttacggaa gtggagaaac tcgacatgcc
     8881 gcagagtttt ggctggcttg ttggcccgta caggtacttt ccgcaatgaa aattcgtcag
     8941 gcgcagacca gcgcaaccta cattctgccg gaccccggcg aactgaataa acgcgtcctg
     9001 atccgccagc gggtggatat gcccgcggat acctttggcg tggagcctca atacccggtt
     9061 acgttccgga catgggcgaa ggttatccag accagtgcca ccacctggca ggaaaccgcg
     9121 cagaccgggg acgccatcac ccattacatc accattcgtt accgccgggg gatcaccgct
     9181 gattatgagg tggtctgcgg tgacagtgtg taccgggtga aacgtcagcg cgatctgaac
     9241 ggggcgcggc gctttctgct gctggagtgt acggagctgg gcgagtttac gcagagtcac
     9301 ggaggcagca atggcgactc ccttttttca cgttgatgtt cagcagcccg cggaaatgcg
     9361 ctttaaccgc gcccgtgtcc ggcgggcgtt tatcacgatt gggcagcgtc atatgcgtga
     9421 tgcccgtcgg ctggtgatgc gccgtgcgcg gtcggcaccg ggtgaaaacc ccggttatca
     9481 gaccggacgc ctggctcgtt cgattggtta catggtgccg agagccagta aaaagcgagc
     9541 cggttttatg acacgcattg cccctaacca gcacaacggg aaggggaacc ggatgatctc
     9601 tggtgacttc tatccggcgt ttctgttttt tggtgtccgg ggaggagcaa agcgtcgtcg
     9661 cagccatcat cgtggtgcat ccggtggcag cggctggcga ctggctccac gtaataactt
     9721 catggtggaa acgcttgaaa agaaccgcag ctggacacgc tattttctgg cgcgggaatt
     9781 gcgtaaatca ctgaagccgg agcgacgaca cagatgaaac tgacgcctgt tattgctgca
     9841 ctgcgtgccc gctgtccgta ttttgaaaac cgggttgcag gcgcggccca gttcaaaaat
     9901 ctgccggagg tcggaaagct gaaactcccg gcggcatatg ttgtaccggg tgatgattct
     9961 ccgggagaaa acaaaagcca gaccgactac tggcaggagc tgaaagaggg tttctccgtg
    10021 gttgtcatac tgagtaacgg gcgtgatgag cgcggtcagt ttgcctcgta tgatgtggtg
    10081 gacgatgtcc ggcagatgct ctttaaggct ctgctgggct ggaacccgga ggcgtgcggt
    10141 aacccgatta cctatgacgg cggcacgctg ctggatctga atcgtcatga gctgatttat
    10201 cagttcgatt tttcggtcat cagcgagctg actgaagacg atacccgcca gcaggatgat
    10261 ctgaacagtc tggatgaact gcaaacgctg gcgattgatg ttgattatct cgagcccggt
    10321 aacgggcctg acggcgatat cgaacatcac accgaaatac cccttccttc ctgaggatcc
    10381 tcatgtttgt caaacctgtt aaagggcggt cagttcctga ccctgcccgc ggcgaccttt
    10441 tgcccgccga agggcgaaat gttgacgaga acaactactg gctgcgccgt gaagcagcgg
    10501 gtgatatccg gcgcgtgaat aaaaaggtga ataccgatga cgataagctt taacaccatt
    10561 ccgtcgaata cgctggttcc gttgttttat gcggaaatgg ataaccaggc ggcgaatact
    10621 gcacaggaca gcggagcatc gctgctgatt ggtcatgcca ataacggtgc agagattgtt
    10681 gccaacagtc tggtactgat gccgtcggca gactatgcac gccagatttg tggtgcggga
    10741 agtcagctgg cgcgtatggt cgaggcttat cgccagactg acccgtttgg cgagctgtat
    10801 gtgattgccg ttcctgaatc cacaggcgcg gcggcaacgg ttacgctgac ggtgaccggg
    10861 gcggcaaccg aaaccggcac ggtgaatgtc tatgtgggac gtacccgcgt gcaggcaccg
    10921 gtgactaacg gcgataacgt cacgatgatt gccagcagta tccaggatgc catcaatgcc
    10981 gttccggccc tgccgtttac ggcttcatct tcggcaggcg tggtcacact gaccgcgcgt
    11041 cataaggggc tttgcgggaa tgaaattcct gtcagcctca attactacgg ctttggtggg
    11101 ggcgaagtgc tgccagcggg cgtacagatt gccgtggcga cgggtaccgc cggaacgggt
    11161 gctccggttc tcaccggcgc ggtggctgca atggcggatg agccgtttga ttatatcggc
    11221 ctgccgttca acgacacggc ctccgttaac acgctggtga ccgagatgaa cgataccagc
    11281 ggtcgctgga gctatgcgcg tcagctgtat ggtcatgtgt atacggcaaa gatcggcacg
    11341 ctgtcagaac tggtgaccgc aggtgaccag tttaaccagc agcacattac cctggcgggg
    11401 tacgaaaaag agacccagac gcctgccgac gagctggcgg caagccgtac cgcccgcgca
    11461 gcggtgttta tccgcaacga tccagcacgt cccacgcaga ccggtgagct ggtgggtatg
    11521 ctgcctgcgc cgaaggggaa acggttcacg atgaccgaac aacagaccct gctgtctcat
    11581 ggcgtggcaa cggcgtatgt cgaaagcggg gtactgcgca ttcagcgtga tgtcaccacg
    11641 tacaggaaaa acgcttacgg ggttgcggat aacagctacc tcgacagcga gacgctgcat
    11701 accagtgcgt atgtactgcg caaactgaaa tccgtcatta ccagtaagta cgggcgtcac
    11761 aagcttgcca gcgacggtac ccgctttggt cccggtcagg cgattgtcac cccggcggtg
    11821 atcaaagggg aactgctggc aacctaccgt cagcttgagc gtgcggggat cgtggaaaac
    11881 tacgaactgt ttaagcagta cctggttgtg gagcgtgatg ccagcgatcc gaaccgcctg
    11941 aacacgctgt tcccgcctga ctatgttaac cagttgcgtg tctttgccgt ggttaaccag
    12001 ttccgtcttc agtattcaga ggagtctgca taatggcccg tatcggggga acctgttatt
    12061 tcaaaattga cggtcagcag ctatcgctga ccggcggcat tgaggtgccc atgaacagga
    12121 cggtcaatga tgacatcatc ggcctggacg gttcagtgga ccgcaaggaa actcaccgtg
    12181 cgccttatgt taaagggacc ttcaaggtgc cgaagaattt tccggtgagc aaaatcacct
    12241 cgtctgatga gatgaccatc actgccgagc tggcgaacgg tcaggtctat gtactgtcgt
    12301 ctgcctggct gcacggcgaa gcgaaccata atgccgaaga agggacggtt gatcttgagt
    12361 tccacggtga agaaggggat taccagtaat gaaagagctt gagttaaaga aaccgattac
    12421 cgctcatggc gagacactct ccgtactgga gtttgatgag cccaccggga aagatgtccg
    12481 cgagctgggg tatccctacc agatgaatca ggatgagtcc gtcagacttc tggcgcatgt
    12541 ggtatcgaaa tacattgtgc ggctggcgaa agtgccgcaa aactctgtcg accagatgtc
    12601 tccggcagac ctgaatgctg cggcgtggct tgtggctggt tttttcctcc aggcctgacg
    12661 gctgaatacc tcactgatcg cttctttgac tgcgccagct actggcgcat taatcccttc
    12721 gaattgctga atatgccgat cagtgaaatt cccttgctgg tcagtcaggc aaacaggata
    12781 gagcaggaga aacgcacaca tggctgaatt tgagcttaag gcgttgatca ccggtgtcga
    12841 caggctttct cccgcgctgt cgaaaatgca aaagaaaatc cggggattta aacgccaggc
    12901 ggaagaagcg tcacagggtg ggttggcgct tggtggcgga ctggctgcgg gtctgacgct
    12961 ttccctgaaa tcttatgccg atcaggaaaa cgccgccacc gggctgaaag ttgccatgat
    13021 ggatgcgaac ggtgaggtcg gaaagcgctt tcaggacatc aataaactgg ctattggtct
    13081 gggtaaccag ctacccggta caacggctga tttccagaac atgatgcaga tgctggtgcg
    13141 tcaggggatc ccggcagaaa acattcttgg cggtgtgggt aaagcgacag cttatcttgc
    13201 ggtacaactg aaaaaaacac cggaagcggc tgctgagttt gctgcaaaga tgcaggatgc
    13261 taccggaacg gcgtcagaag acatgatggg gctgttcgac actatccaga aggcgtttta
    13321 tctgggcgtt gacgatacca acatgttgtc cttcttcact aaaaccagtt ctgttctgaa
    13381 gatggtgaac aaggacggtc ttcaggctgc acagagcctt gcccccatca gcgtcatgat
    13441 ggatcagatg gggatgaacg gggagtcggc aggtaatgcc ctgcgaaaag ttatccagtc
    13501 cggattaagc gttaagaaaa tcagggacgt caataaagtc atggcccgcc agaaactcgg
    13561 ggtacagctc gattttactg acggcaaagg aagttttggc ggtcttgata acatgttcag
    13621 gcaactggca aagctgcgaa aactgaccga cgttaagcga acaggtgtac ttaaggcaat
    13681 atttggtgat gatgccgaaa cccttcaggt ggtcaatgca ctaatcgata aaggaaagga
    13741 tggctacgat cagatccagc agaagatgaa taaacaggcc agcctgaata aacgtgttca
    13801 ggcccagctt ggtacgctgt ccaacctgtg ggaggcaatg acggggaccg caactaacgg
    13861 ccttgcggct attggtggcg cattttctgg tgacgccaaa aatatcacgc aatggctggg
    13921 ggagttaggg gaaaaattca cgaagtttgc ggatgaaaat ccccgggtta ttcgcggcgt
    13981 cgtcgggctt gctgccggtc ttgcgattct gaaactggga ttgatgggcg ttggcggtgc
    14041 catcagtatt gtcagcagga tcatgtcgat gacgccgatt ggaatgattg cgacggcgat
    14101 agccctggct gcgggattaa ttatcactaa ctgggatgtt gtcggacctt atttcaagaa
    14161 gctctgggaa accattggtc cttattttga ggctggctgg gaacttctga agaaggtttt
    14221 tgcctggtcg ccgctgggga tggtaatcaa taactgggga ccggttgtta agtggtttca
    14281 ggatatgtgg gacaagctga agccaattat tgagtggttt accgacagtt ccggtgacac
    14341 ggtcgatgcc attaactctg cgcagtgggg cgcgggtgct tatgatgctt atgggacggg
    14401 aataccggcg cggggataca caccttatcc ggcggtggat ccggctcagt caaacaacgc
    14461 ctccggtgcc acaggcccga atcccttcat gattaacaaa gcttctgcgc caaaagttga
    14521 tggtgagatc aaggtctctt ttgtgaattc gcctccgggt atgcgggtta tggaaacgcg
    14581 atccagcggt tttgatgtca gccatgatgt tggctatacg cgctttggca ggtaatgaca
    14641 aattaatctg ttaatgagtc ccactccggt gggatttttt atgtacggag tttatatgac
    14701 gtggaaagac agacttcagg acgcgtcatt tcgcggtgtg ccgtttaagg ttgaagaaga
    14761 aagtgcggga accggtcgtc gtgtggaaac gcacgaatac ccgaaccgcg acaaacccta
    14821 taccgaagac ctggggaaaa tcactttccg cccgtccatc acagcttatg tggtgggaga
    14881 tgactgcttt gaccagcgcg atcgcctgat tgacgcgctg aataaacccg gtcccggcac
    14941 gcttgtccat ccgacttacg gtgagctgaa agtctgtgtt gatggggaag ttcgggtcag
    15001 cacatcgaag agtgaagggc gtattgtccg ctttgacctg aagtttgtcg aagcgggaga
    15061 actctcttac cccacttcag gtgcggcgac ggcgcagacg ctgatgtcat cctgttctgc
    15121 actggatgac tgcatcagtg acagcttcag tggtttcagt atcgatggcg tggcagattt
    15181 tgtgcagaac gacgtcgtcg gtaatgccag cacaatgctt gggtatgttt ctgatgcgat
    15241 gaaagtggtg gattctgccg tatcggatgc cgccagactg ttgcaggggg atatctcggt
    15301 acttctgccg ccgccatcgt caggcaaaaa tttcgttgag caggtgcaga aaatgtggcg
    15361 taccgggaaa cgcctttatg gtaacgccag cgacctggtc accatgatca aaacgctttc
    15421 cggtgtcagc ctcggcagcg atctgcaacc gcgcggcgtc tggaaaacgg acagtaaaac
    15481 caccgccacg gcgacgcagc agcgtaacgt ggttgccagc acccttcgta cgaccgcaat
    15541 cagcgaagcg gcgtatgccg tcacacgatt gcctgcgccc acaacttccg cggtgatgca
    15601 gaatgccgca gtggggcagg caacaacacc cgcgcagagc accggctggc cttctgtcac
    15661 gcatccggca ctgaacaatg caccggcggt gaaaaacacg gttgacctgc caacgtggga
    15721 agaactgacc gacattcgcg acacactgaa tacggcaatt gataaggagt tgtcccgtac
    15781 aaccagtgat gcgctgtttc tggcgctgcg ccgggtgaaa gcagatctga atgcggatat
    15841 caacacgcgc cttgaacagt ctgctcggat cattcagcgc acgccggatg aggttttacc
    15901 cgcgctggtg ctggctgcga cctggtttga taacgcggcg cgtgacgcgg acattatccg
    15961 gcgtaatgcc attacgcatc ccggctttgt gccggtgatc cctctgaagg tgccagtgca
    16021 atgaacgaca atgtcacgct acgggtagat ggccgggagt ggaatggctg gacatcggtg
    16081 cgcatcggtg ccggtattga acggctggcg cgggatttca gtgtggagat cactcgccag
    16141 tggccgggag atgagggtat caccacgctt cagccgcgca ttaaaaacgg ttcaaaagtg
    16201 gaagtgctga ttggtgatga gctggtgatc accggctggg tggaggcgac tcccgttcgt
    16261 tacgatgccc gttcggtcag caccggtatt gccggacgta gtctgacggc tgacctgatt
    16321 gactgtgcag ccgaaccgac acagtttaac ggacgctcgc tggtgcagat tgcgcaggcg
    16381 cttgctgcgc ctttcggcat tgaggtggtg aacagcggtg cgccgtcggg tgttattcct
    16441 gatgttcagc ctgatcacgg tgaaacggtg attgaggtaa tcaacaaaat actcggtcag
    16501 cagcaggcgc tggcttacga cgacccgcac ggcaggctgg tgattggcgg tattggctca
    16561 acgcgggcac ataccgcgct ggtacttggg gaaaacatcc tttcctgtga tacggagaag
    16621 agtatccggg agcggttttc tgtttaccag gtggcggggc agcgtgccgg aaacgacgat
    16681 gatttcggtg aggccaccac caccgcgctg cgggcccgca cagaggacgc atttattgcc
    16741 cgttaccgtc cgatgtatat caggcagaca gggcaggcca cgggggcagg ctgtattgcg
    16801 cgtgctgact ttgaagcccg aaaacgggcg gcgcggacgg atgaaaccac ctatgtggtg
    16861 cagggctggc gacagggtaa cggtatgctg tggcagccca accagcgggt gattgtcttc
    16921 gatccggtct gtggtttcga caataccgaa ctgcttgtct cggaagtcac gtttactcag
    16981 gaccagaacg gcaccctgac ggaaatccgt gtcggcccgc ctgatgctta tctgcctgaa
    17041 cccgaagccc ccggcgcgcg gaaaaagaaa aaagccagag tacaggagga cccgttctga
    17101 tgaggacgat tgaagccatg cagcgacaac ttctcggcct gattgggcgg gccgtggtga
    17161 aaagcatcag tgccgccacg aaatgtcaga ccgtggatgt gtccctgatt gccggtgaac
    17221 ccaaagctgg ggttgaacat cttgaaccct acggttttac cgcaagggca aacagcggtg
    17281 cggaagcggt ggtgttgttt ccggatggcg accgttctca tgcggtggtt gttacggtgt
    17341 cggaccgacg ctaccgccta aaagggctgc agacgggtga ggtggctgtc tatgacgatc
    17401 aggggcagtc tgtgacgctg acccgggagg ggatcgtggt ggacggtgca gggaaaacga
    17461 tcacgtttcg caatgcacct gaagcacgtt ttgaaatgga tctggaagtg accggacagg
    17521 tgaaagacct gtgcgactcc ggcggcacca ccatgtcagc gatgcggctt gcctataacg
    17581 ggcatcgtca cagagagaac ggtcagggca gtaacaccga caaacctgat aaagcgatgg
    17641 aggcatgatg gaactgtggc tgacggtgaa cggtaaacgc acctgcgcca gcgcaccgct
    17701 ggatccgctg acccgcgccg tggtgatttc cctgttcacc tggcggcggg cggagcctga
    17761 tgacaatgcc gacgtcccga tgggatggtg gggggatacc tggcctgcgg tacagaatga
    17821 ccgttacggc tcccgactgt ggctgcttca gcgcagcaaa ctgaccaatc agctggtgct
    17881 gacggtaagg gggtatatcc gcgaatgcct gcaatggatg attgatgacg gcgtggtgtc
    17941 ccgtattgat ctggatatcc gccgcaccgg gattaatgaa ctgggtaaca gtatcactct
    18001 ctggcgtcgt gacggaccgg taatgatttc ttttgatgat ctgtggagtg cgataacgca
    18061 tggcggacag tgaatttcag cgcccgacgc tggcagaaaa tatcagtatg ctccgtaacg
    18121 atttattcgc caggctggac gtcagcgaca cgctccggcg catggatgaa gacgtgcggg
    18181 caaaggtgta tgcggcggcg ctgcatacgg tttacgggta catcgattat ctggcaatga
    18241 acatgctgcc tgacctgtgc gatgagtcct ggctggcgcg acatgctgcg atgaaacggt
    18301 gtccgcgcaa gggggccacg gctgccagcg ggtatatgcg ctgggaaggt gtcagcgatg
    18361 gcctgaaagt gaccgccggg agcgtgattc agcgcgatga cctggttcag tacacggcaa
    18421 ctgccgatgc aaccagctcc ggtggtgtcc tgcgcgtgcc gatcgcctgc tcaagtgcag
    18481 gcgcggtcgg taacgctgac gacggtacgg cattaatcct ggtcacgccg gtgaatggtc
    18541 tgccgtcttc cggtgtggct gacactctga caggcggatt tgatactgaa gatctggaaa
    18601 cgtggcgcgc ccgcgtcatt gagcggtatt actggacgcc gcagggcggg gctgacgggg
    18661 actatgtcgt ctgggctaaa gaagtacccg gcattacccg cgcatggaca taccgtcact
    18721 ggatgggaac gggaactgtc ggtgtgatga ttgccagcag tgacctgatt aatcccattc
    18781 cggaagaatc aacggaaacg gcggcaagac aacatatcgg gccactggcc ccggtggcag
    18841 gctctgattt gtatgtgttc aggccggtgg cacatacggt ggattttcat atccgtgtga
    18901 cgccggacac accggaaata cgggctgcca tcaccgcgga gttgcgttcg ttcctgctgc
    18961 gtgatggtta tccgcaggga gaactgaagg tgtcacgtat cagtgaagcg atttccggtg
    19021 cgaacgggga atacagccat cagttgcttg caccggcgga caatatcacc attgcgaaaa
    19081 atgaactggc ggttctgggg gcgatttcat ggacgtgaca aacgatgatt acatccgtct
    19141 gttgtcagca ctgttgccgc ccggtccggc gtggtcagcc agcgatccgg cgattgccgg
    19201 tgcggcaccg tcattaaccc gcgttcatca gcgtgcggat gccctgatgc gggagctgga
    19261 tccgcgcacc accaccgaac tgataaatcg ctgggagcgt ctgtgcggcc tgccggatga
    19321 atgtattcct acagggacac agacccttcg ccagcgtcag caacggctgg atgcgaaggt
    19381 taacctggcg ggcggcatca acgagaattt ttatcttgca cagcttgctg ccctgggcag
    19441 accggatgcc aacatcacgc gatacgacaa aagcacgttc acctgctcat cggcctgtac
    19501 tgacgcggtg aatgcgcctg aatggcggta ttactggcag gtcaacatgc cagccgccac
    19561 caacaccacc tggatgacat gtggcgatcc ctgtgattcc gcgctgcgct tctgggggga
    19621 caccgttgtc gagtgtgttc ttaacaaact ctgcccgtcg catacctatg tgatttttaa
    19681 atatccggag taatccatgc atcgtataga cacgaaaacc gcgcagaagg ataagttcgg
    19741 cgcgggtaag aacggtttta cccgtggtaa cccccagacc ggcacgcctg ccaccgatct
    19801 ggatgatgac tactttgaca tgttgcagga ggagctttgc agcgttgtgg aggcatccgg
    19861 tgccagcctg gagaaggggc ggcatgacca gctgcttacc gcgcttcgtg cgctgctgtt
    19921 aagccgcaag aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cagctctcga
    19981 aaa
//
LOCUS       DAPPNJ010000076        19878 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000076 DAPPNJ010000000
VERSION     DAPPNJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.076"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(74..745)
                     /gene="queE"
                     /locus_tag="RPM73_002250"
     CDS             complement(74..745)
                     /gene="queE"
                     /locus_tag="RPM73_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDX6520604.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            884..1024
                     /locus_tag="RPM73_002251"
     CDS             884..1024
                     /locus_tag="RPM73_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520605.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1038..1910
                     /locus_tag="RPM73_002252"
     CDS             1038..1910
                     /locus_tag="RPM73_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDX6520606.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNSQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1970..3268)
                     /gene="eno"
                     /locus_tag="RPM73_002253"
     CDS             complement(1970..3268)
                     /gene="eno"
                     /locus_tag="RPM73_002253"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDX6520607.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3356..4993)
                     /gene="pyrG"
                     /locus_tag="RPM73_002254"
     CDS             complement(3356..4993)
                     /gene="pyrG"
                     /locus_tag="RPM73_002254"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDX6520608.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5221..6012)
                     /gene="mazG"
                     /locus_tag="RPM73_002255"
     CDS             complement(5221..6012)
                     /gene="mazG"
                     /locus_tag="RPM73_002255"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDX6520609.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6083..6418)
                     /gene="mazF"
                     /locus_tag="RPM73_002256"
     CDS             complement(6083..6418)
                     /gene="mazF"
                     /locus_tag="RPM73_002256"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDX6520610.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(6418..6666)
                     /gene="mazE"
                     /locus_tag="RPM73_002257"
     CDS             complement(6418..6666)
                     /gene="mazE"
                     /locus_tag="RPM73_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDX6520611.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(6744..8978)
                     /locus_tag="RPM73_002258"
     CDS             complement(6744..8978)
                     /locus_tag="RPM73_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDX6520612.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(9026..10327)
                     /gene="rlmD"
                     /locus_tag="RPM73_002259"
     CDS             complement(9026..10327)
                     /gene="rlmD"
                     /locus_tag="RPM73_002259"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDX6520613.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPKLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10384..13140
                     /gene="barA"
                     /locus_tag="RPM73_002260"
     CDS             10384..13140
                     /gene="barA"
                     /locus_tag="RPM73_002260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDX6520614.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(13372..14712)
                     /gene="gudD"
                     /locus_tag="RPM73_002261"
     CDS             complement(13372..14712)
                     /gene="gudD"
                     /locus_tag="RPM73_002261"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDX6520615.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14733..16073)
                     /locus_tag="RPM73_002262"
     CDS             complement(14733..16073)
                     /locus_tag="RPM73_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDX6520616.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(16075..17427)
                     /gene="gudP"
                     /locus_tag="RPM73_002263"
     CDS             complement(16075..17427)
                     /gene="gudP"
                     /locus_tag="RPM73_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDX6520617.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17862..18311)
                     /locus_tag="RPM73_002264"
     CDS             complement(17862..18311)
                     /locus_tag="RPM73_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDX6520618.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(18329..19111)
                     /gene="truC"
                     /locus_tag="RPM73_002265"
     CDS             complement(18329..19111)
                     /gene="truC"
                     /locus_tag="RPM73_002265"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDX6520619.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(19111..19440)
                     /locus_tag="RPM73_002266"
     CDS             complement(19111..19440)
                     /locus_tag="RPM73_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDX6520620.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4901 a         5334 c         4684 g         4959 t
ORIGIN      
        1 gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct
       61 tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat
      121 tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct
      181 gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc cagtagttca tccagtgctt
      241 caatatcacg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg
      301 acaacacttc atagccgccg cgcatgttca gctttggtga tacggtaacc caggtattcg
      361 gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa ccgttctttt
      421 cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca
      481 catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca ctgctcgccg
      541 ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat
      601 cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct
      661 gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga
      721 acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt
      781 gcagatcctg ccgcaacaat catgtcttat taacattctg ttacatgcag atttaaagaa
      841 aaatgcgaaa catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa
      901 agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt
      961 agtttttaat gataacgaat ctaaaattac cgatgccgcc gtcaatttat ttatccagat
     1021 ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgta tgttcctttg
     1081 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacggat cttacgagta
     1141 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
     1201 cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca attgaacaat
     1261 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
     1321 tgatcattgt tgcctggtca gatcgtactg tccgcatcca ggtaggttat gggctggaag
     1381 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
     1441 aacaacaaaa atttgctcag ggattagagc tagctataaa cgctttgaat agccaactca
     1501 cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct tccagtgatc
     1561 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
     1621 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatctatc
     1681 ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt tccttctttt
     1741 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatcag
     1801 gtaaaagtta tcattcagac aacagcggtt ccggtggagg ctcaagtggc ggcggattta
     1861 gcggcggggg cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa ctcaaccata
     1921 aaaaatgcca gcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc
     1981 ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa
     2041 tcagctggtt gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc
     2101 ctgcagcagt accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt
     2161 gagagataac tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg
     2221 tcagagaacc gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac
     2281 cttctttcag gatcttggtg ttagttacga acaggtcgtc accaaccagc tggattttgt
     2341 cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt
     2401 cgatagatac gatcggatac tgtttggtca gttcttccag gaagtgagtg aattcttcag
     2461 aggtgaaagc tttgttgcct tcgccagcca gaacgtattt accgtctttg tagaattcag
     2521 aagctgcgca gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa
     2581 cagcttcagc gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac
     2641 cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga
     2701 aaacttcaga acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca
     2761 tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca
     2821 tcggaaccgg catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca
     2881 gcggcatacc tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg
     2941 cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa
     3001 tgccagcctg atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg
     3061 cagcaacagc tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt
     3121 ccagagcttc acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac
     3181 caccctccag atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac
     3241 gaccgatgat ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa
     3301 actccagaca aacaacgcgt acccagggta cgcgttgccg ttctaacttt tttacttact
     3361 tcgcctgacg cttctggaac tcgctggcgg cttttacaaa gccagcaaac agcgggtgac
     3421 catcacgtgg agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat
     3481 tcggaacttc gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac
     3541 ctgcatcttc aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct
     3601 caacaattgt cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact
     3661 gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt
     3721 tgccgttttc atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca
     3781 caaattccgt agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg
     3841 ccacctgcat acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg
     3901 cggtcgtaat catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt
     3961 ccagaccttt aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga
     4021 tgttgacgct gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat
     4081 aagcatccgg cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt
     4141 tcgcttcttc gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc
     4201 tgaatcgttt acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat
     4261 agatggaatc gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg
     4321 caatcttcgc acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag
     4381 gctggatacc gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt
     4441 caccagacgc tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc
     4501 caatttcaac agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta
     4561 ctgtaccgcc gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct
     4621 ctttgattgc gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc
     4681 ggcgttcttt acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc
     4741 tcattttggt acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt
     4801 cttcagtaac gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga
     4861 tgtaaggatc cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga
     4921 gggaggctgc ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat
     4981 agttcgttgt catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac
     5041 gggaaagcag tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg
     5101 ccagcgcact attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac
     5161 gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc
     5221 ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc
     5281 aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg
     5341 ctcaaatttt tcgttcgctt tttgcaatgc gatttccgct tttgtcccta aatgacgagc
     5401 cagattgacc gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac
     5461 aacagcctgc cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcgactac
     5521 cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc
     5581 acgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg
     5641 cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc
     5701 agaactatca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca
     5761 aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg
     5821 gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc
     5881 cagcacttcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt
     5941 atcccacggg cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc
     6001 gatttgattc attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca
     6061 gaatagaagt gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt
     6121 gtaattcctc tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgatac
     6181 tttttacctg atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga
     6241 acggatatcc ttttgattgc gttgtataag gaacacacag acacatacct gttttgttgt
     6301 tgtacatgaa aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg
     6361 tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta
     6421 ccagacttcc ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt
     6481 gatgtcgttg accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat
     6541 taatttgcca tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg
     6601 cattaacgta gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg
     6661 gatcataacc ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat
     6721 tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc gacgcgcgtc gataacatcc
     6781 ggcacctggt tgagtttacc cagcacgcgc cccagcactt gcaggttgta aatctcaatg
     6841 gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg
     6901 ttcaccttct cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta
     6961 gccaccacgc ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca
     7021 acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt
     7081 actgaaatac cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg
     7141 cagcagcgcg cgatgtggtg catcaggttg ccaacacctt cgaccaccac gcgaccgtta
     7201 tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg
     7261 tcctgctctt cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg
     7321 agacggatat ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa
     7381 cgcggcagca gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg
     7441 tcaaggattt gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg
     7501 tgaattttcg aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca
     7561 cggctggggt tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg
     7621 taggtgaacg gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca
     7681 ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca
     7741 cctttcggcg taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc
     7801 atttcgccgg aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc
     7861 cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc
     7921 gcagcaacac ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg
     7981 gttttgccac ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg
     8041 ttagcgacat aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc
     8101 agtgcggcat aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac
     8161 agctcatcga aggcgaggtt ctttttctgc attttgcgcc agatactgta gatgtgtttc
     8221 ggacgaccat acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatggccg
     8281 acaaactctt cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg
     8341 cgtttgtatt cggtcgggtg gagataacgg aagcagtaat cttccagctc ccatttcagt
     8401 tgcccaatcc caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc
     8461 agtacgcgtt catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc
     8521 agtttgatga ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta
     8581 tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc
     8641 atatcacgca cgccgtgaat aaggttaacg accgacttac cgacgctctc acgcagcaca
     8701 tcttcgctga ctacgttggc atcagccaga gggaacagca gcgccgcccg cagcgtgtca
     8761 atgtccatac ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga
     8821 ctggcatccg gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac
     8881 tcacacgact tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca
     8941 ccagccttat tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag
     9001 acctgccgaa atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca
     9061 gatgtcccgt gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt
     9121 ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac
     9181 gaataggttc cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt
     9241 ccagcaacac tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa
     9301 gattttcgtg ataaaacgtc acattctgta aaccattaag acgcgcattc tgctggcctt
     9361 tttccaccag cgccggaaca ccttctacac cgaccacact ggctgcttgt ttcgccaatg
     9421 gcagtgtaaa gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat
     9481 ccagccattc caacgcacgc gctaccattt tttggttcac acccgcattg acctgaataa
     9541 aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc atctcaccag
     9601 agacggtttc gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg
     9661 aaaagcgttc cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca
     9721 tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc aagatggcgc ataacttgta
     9781 agctgcccag gcatgccctg actttgggca gcaatgcttc aagtttgggc actaaaatgg
     9841 ggcattgttt aacgtcgaca atgtcactgg agcccgcttt gcgaaagccc atctgaagtt
     9901 gctgtgtttt cggtaagtag ttcaaactta aacgcgcgcg acggcgatag ccccagggaa
     9961 catcggcgat cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc
    10021 ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca gccaccgcat acgccaaaat
    10081 gagggcagcg tggcgtttcg cgttccgggc tatcgcttaa ccggcgcacg actttagcgc
    10141 gggcgtactg ttttttatct tcagtaacag taacttccgc gttttcctgt ggcagtaatc
    10201 caggaataaa taacgctttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt
    10261 cgttgactga aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt
    10321 gcgccattgg cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac
    10381 tccatgacca actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc
    10441 cttattggtt tattgctgag tatctttttc gtcgtgcatc gctataacga cttgcagcgt
    10501 caactggaag atgccggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc
    10561 atgagcctgc aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat
    10621 tccgatattg ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc
    10681 aattttcatc ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag
    10741 ctcactgtca ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt
    10801 tactcccccg acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat
    10861 attgcgcttg agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt
    10921 tccagcgtga tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg
    10981 cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg
    11041 caactcgaca gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt
    11101 atcaactcga tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac
    11161 caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg
    11221 gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat
    11281 atgtcacacg agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg
    11341 aaaacagaat taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat
    11401 aatttgctgg caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg
    11461 attctggaaa gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg
    11521 gcacattctt ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct
    11581 gataacgtga tcggcgaccc gctgcgatta cagcaaatca tcactaacct ggtggggaat
    11641 gcaattaaat tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt
    11701 aataccaaag tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc
    11761 gatcaatcgc gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat
    11821 ggtggcaccg ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat
    11881 atttcgttcc atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat
    11941 ctgaacccga acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg
    12001 gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg
    12061 ccgctggaag tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg
    12121 atgttgttag gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta
    12181 gcgaaagcgg tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc
    12241 aatgctgaaa aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct
    12301 acacgcctgt tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct
    12361 gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac
    12421 ctgaaactta tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc
    12481 gggcatcagg cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt
    12541 caaatgcctg acatggatgg cattcgggcc tgcgagctca tccaccagct cccccatcag
    12601 caacaaacgc cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg
    12661 cttggcgcag ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat
    12721 ttgttgttgc gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc
    12781 aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca
    12841 gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa
    12901 gttcgcaaca aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg
    12961 attcataaac tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc
    13021 caactgatcg aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg
    13081 ctggaactgt tggacgagat ggataatgta gcgcgcgaag ccagcaaaat tctcgggtaa
    13141 cggacgtctt gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa
    13201 gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa
    13261 attcaatgaa ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt
    13321 tagcatcagt ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac
    13381 catgcacggg cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc
    13441 atcgtcacgc gcgccaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc
    13501 catatcgatt tctacaccca gccccggttt ttctggcacc tgtaccagcc cgcctttgat
    13561 ctcaaacggt tctttggtca ggcgctgatt accttcctgc cagatccagt gcgtatcaat
    13621 agcagtaatt ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc
    13681 gaagtggttg tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac
    13741 acgtaccgaa ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg
    13801 cagggagagc gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg
    13861 caggccagtt gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc
    13921 accacacgga tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc
    13981 ttcgttcagc gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg
    14041 cgccagtgcc acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt
    14101 gaaatcgttg aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg
    14161 cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg
    14221 atacggcagc ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac
    14281 ttcgctacgc tgttgaccat cgcccagcag ggatgccacg tttaccccca gatgctgccc
    14341 cagcagatcc agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc
    14401 aaatgtctgc aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt
    14461 cagaacgttt ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag
    14521 cgttttacgg attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc
    14581 tttgataatc acaatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag
    14641 catactgtca tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa
    14701 ttgagaactc atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta
    14761 cggtcaaagg tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg
    14821 acaggcagac gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg
    14881 cccagaccgg gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg
    14941 gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc
    15001 ggtgccgccg cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa
    15061 tggcagcccc aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa
    15121 agcgtccaga agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga
    15181 cccatttcgc gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc
    15241 cgaaactccg ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg
    15301 gcataggtaa gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag
    15361 gcaccgttgg gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca
    15421 gtgtcgattt cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca
    15481 tagcgatcct gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga
    15541 tggcgcaact gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt
    15601 ttggtccgat caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg
    15661 cctggcccca acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag
    15721 gcggcttcca gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg
    15781 aaggtatcaa aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg
    15841 cgcgcaactt cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc
    15901 tctccgcccg gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata
    15961 ttgcgggtga aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg
    16021 gcgaccggaa tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg
    16081 ccccgcgaca ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag
    16141 taccgcgatt aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt
    16201 gccaacgata taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt
    16261 gaacaggcca ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca
    16321 acccagcgcc ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca
    16381 ctcaacgttg acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat
    16441 cggtgtttta cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat
    16501 cccacccagc acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc
    16561 tttcagaatc gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa
    16621 gtaagtcagg gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc
    16681 gagcagttgt ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg
    16741 ctgatccata ttgatcagcg cgccacccgc ggcgatatac tccagctctt tctgatttac
    16801 ccccggatgt tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc
    16861 cagaccgccc ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat
    16921 aatcggcgcg aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt
    16981 tcccctttcc tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg
    17041 cgcttcagca agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc
    17101 actaaagata tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca
    17161 gaagtagacg cgttttgaac caaaacggtc cagcaaccag ccaccaggga tctgcccgat
    17221 aacataagcc catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat
    17281 atctttggcc atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga
    17341 tgtgacgata aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact
    17401 gctcgcagcc tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc
    17461 tcactctgga ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt
    17521 tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag
    17581 cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt
    17641 ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag
    17701 gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa
    17761 cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga
    17821 ttcagcaccg ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc
    17881 cactgttcga cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc
    17941 aacagtattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg
    18001 ccgccattgc agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga
    18061 ttcggctgga agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga
    18121 aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc
    18181 cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt
    18241 gcttctgctt cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca
    18301 atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg
    18361 gcgcactgaa ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata
    18421 acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag
    18481 gatgcgtcaa tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag
    18541 caccgctgcg attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca
    18601 aatgggcaag atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca
    18661 ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac
    18721 cgcgataatg cgtcactgct ggctgcgagc ctttatcttc gcgggcaaat ttatcagcga
    18781 ttttgtccag ttcttccacc agtggataat ccagaactgc ttcttccatc aaccagccgc
    18841 gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc
    18901 gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat
    18961 gagcggtaaa aacgtgctgg ccaatctgat cacgcacggt ttgcatgacc actactttct
    19021 cgtcgcgatc cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca
    19081 gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc
    19141 cagtttttcc agctcagcca gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat
    19201 ttcataatag ggtgcaacgg caaaagcgcc cggtaacggc tgtttgttat ctaacaaatc
    19261 gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa
    19321 gaacggttgg gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg
    19381 ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat
    19441 aaagcaacct caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac
    19501 tgtattcaca gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc
    19561 tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt
    19621 cctgctcaca ccaccccgat gtgatacttc atcctgaagt gtccctggcc ttcctgaccc
    19681 accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg
    19741 cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg
    19801 cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga
    19861 atcgtctttt tcgcgaag
//
LOCUS       DAPPNJ010000077        19558 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000077 DAPPNJ010000000
VERSION     DAPPNJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.077"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(217..1488)
                     /gene="efeB"
                     /locus_tag="RPM73_002267"
     CDS             complement(217..1488)
                     /gene="efeB"
                     /locus_tag="RPM73_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDX6520621.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(1494..2621)
                     /gene="efeO"
                     /locus_tag="RPM73_002268"
     CDS             complement(1494..2621)
                     /gene="efeO"
                     /locus_tag="RPM73_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDX6520622.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2679..3509)
                     /locus_tag="RPM73_002269"
     CDS             complement(2679..3509)
                     /locus_tag="RPM73_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDX6520623.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(4051..5559)
                     /gene="putP"
                     /locus_tag="RPM73_002270"
     CDS             complement(4051..5559)
                     /gene="putP"
                     /locus_tag="RPM73_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDX6520624.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(5718..5927)
                     /locus_tag="RPM73_002271"
     CDS             complement(5718..5927)
                     /locus_tag="RPM73_002271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520625.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            5982..9944
                     /gene="putA"
                     /locus_tag="RPM73_002272"
     CDS             5982..9944
                     /gene="putA"
                     /locus_tag="RPM73_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDX6520626.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELTEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(9984..10622)
                     /gene="rutR"
                     /locus_tag="RPM73_002273"
     CDS             complement(9984..10622)
                     /gene="rutR"
                     /locus_tag="RPM73_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDX6520627.1"
                     /translation="MTQGAVKTKGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            10910..12001
                     /gene="rutA"
                     /locus_tag="RPM73_002274"
     CDS             10910..12001
                     /gene="rutA"
                     /locus_tag="RPM73_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDX6520628.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            12001..12693
                     /gene="rutB"
                     /locus_tag="RPM73_002275"
     CDS             12001..12693
                     /gene="rutB"
                     /locus_tag="RPM73_002275"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDX6520629.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            12705..13091
                     /gene="rutC"
                     /locus_tag="RPM73_002276"
     CDS             12705..13091
                     /gene="rutC"
                     /locus_tag="RPM73_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDX6520630.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            13099..13899
                     /gene="rutD"
                     /locus_tag="RPM73_002277"
     CDS             13099..13899
                     /gene="rutD"
                     /locus_tag="RPM73_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDX6520631.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            13909..14499
                     /locus_tag="RPM73_002278"
     CDS             13909..14499
                     /locus_tag="RPM73_002278"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDX6520632.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            14510..15004
                     /gene="rutF"
                     /locus_tag="RPM73_002279"
     CDS             14510..15004
                     /gene="rutF"
                     /locus_tag="RPM73_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDX6520633.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            15025..16353
                     /gene="rutG"
                     /locus_tag="RPM73_002280"
     CDS             15025..16353
                     /gene="rutG"
                     /locus_tag="RPM73_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDX6520634.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(16436..16609)
                     /locus_tag="RPM73_002281"
     CDS             complement(16436..16609)
                     /locus_tag="RPM73_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDX6520635.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            16982..17578
                     /gene="wrbA"
                     /locus_tag="RPM73_002282"
     CDS             16982..17578
                     /gene="wrbA"
                     /locus_tag="RPM73_002282"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDX6520636.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            17599..17826
                     /locus_tag="RPM73_002283"
     CDS             17599..17826
                     /locus_tag="RPM73_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDX6520637.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(17864..19105)
                     /gene="agp"
                     /locus_tag="RPM73_002284"
     CDS             complement(17864..19105)
                     /gene="agp"
                     /locus_tag="RPM73_002284"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDX6520638.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         4552 a         5283 c         5110 g         4613 t
ORIGIN      
        1 gacattagtt tctactggaa agatgacaga gtgatgacag atatgaaaaa agctgtgtgc
       61 tttcagcagg attcgcatca ggcatctgcg cacgaacgcc ggatgcggcg tgaacgcctt
      121 atccgcctac cgaacccgca ccgattgtag gcataagaag acgcgcaagc atcgcatcag
      181 gcatctgcgc aagaacgcct tatccgtcta taaacattaa acccgcaata acgcgcttcc
      241 gagataatcg ttcgcgtcct tcactcccgg cagcgcaaaa aaataaccgc cgccgatagg
      301 tttaacgtat tcctccagcg cttcgccatt gagccttttt tgtacagtca ggaagccttt
      361 ttccagatcg tgttggtaac agacaaacag tagccccata tccagttgcc cggagttggt
      421 gacgcccagt gaataactgt agccacgacg cagcatcagg ctggactcac tctccgccgt
      481 gcggggattc gccagccgga tatggctgtc cagcgcaatc accttccctt ccggatcgct
      541 ggcgtaatcg ggaacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc
      601 acgaccaaaa atcgtctgct gttctttcag cggagttctg tcccaaaatt ccactcgaaa
      661 ctgaatcaag cgtactgcct gatagctgcc accgattgtc cacgctggct cctgctgatc
      721 tgccgttacc cacaccactt tttgcatcaa cttatcattc tggctatcag gattggcagt
      781 gccgtctttg aaacccagca aattaatcgg cgtctctttg cctttactac gcgccgcgtg
      841 atcggaaata aacccttccc gcttccagcg cacgctgagc aaatctggcg tatgtttgat
      901 gatatcgcgc agcgcatgga tcaccgtgtc ctgggtgttg gcgcaaattt gtagcaacac
      961 atcaccatga cataacgccg catccagcga gtcgttggga aaacgcgtca tcttctgcaa
     1021 ctttttcggc atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga
     1081 taacgtgatg gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg
     1141 tggcaggcgc ggatttggcg tttctggtgc cgctccgccc tgagtcagaa aagcaaaacg
     1201 ctgagtcaac aagcgaaaca accgttcaag atcgggttta tcgctggcaa gcacatcaaa
     1261 cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata
     1321 aaacggctgt ttctcattgc gtgcatccgg tgaaagcgta cccggcgcac tttgcgtttt
     1381 ttgtgcatga gcgaccggac aacttcccgc cagcgccagc gcaccaatgc ctttcagtaa
     1441 acgtcggcgt gacggttcat tcacgccgtt tttatcttca tactgcataa tgcttaatcc
     1501 aatcccagca caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc
     1561 agtgcattcc ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg
     1621 tatttcgcca gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc
     1681 gggttggctt tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg
     1741 ttagcctgga aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg
     1801 ctggctgcca cttcctcaat cagtccggct gcaccgccga ccacttttga aggtgggaaa
     1861 gccagttcac tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac
     1921 tgatccatgc ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaaccg
     1981 gtgaatttcg gatcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca
     2041 tccagatcgg agaacagttc agcaatcggt tcgatgcgct catagtgctg gcgcgtcggc
     2101 gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa tagcgtcagt aaaggctttc
     2161 gtgtcggtca ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca
     2221 ccaccaagac ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg
     2281 atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt
     2341 aaattcgccg tcattttctg gctaaagcca ggggcgatat tttcccgctc ttccaccacc
     2401 atcacgcctt tgaggatctc ccactccagc gccttctggc tgtggttctg aataatgaac
     2461 tgtgttttcc cggcgttaac cgtaatggtc atcggttcgc actgcttatc ggtcacggtc
     2521 accttgacct gcggcacatt agcggcgtta gccataaaag cagaagagaa cagcgcagcc
     2581 acgctcaact gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa
     2641 agtatgttgt aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc
     2701 gcccctgcgc gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag
     2761 acggcgactt cgctgacgct cggcgcttcc tgatagccaa aaatcccttc catcagcgtg
     2821 ccaaacagcg agtgagttga gagcaccgca ctcatatcga aggcgatttc ctgaaagtgg
     2881 ttccacaatc cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg
     2941 aagagaataa acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc
     3001 cagtagagca ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc
     3061 ggccagatcc cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct
     3121 tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc
     3181 tgcaatgcgc tatcgactgc ctgctccagt tgtactttga cgttgcgcga cactttgcgc
     3241 atccagaaaa ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac
     3301 agttcctgct ctttttgcgg aaattcgccg gtggtttcgt taatgaagat ccccaagccc
     3361 aggcacaacg cagcggcaag caacacgcca atccacatca caccaatcca tcggcctcgc
     3421 tgggtacgct taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg
     3481 cgcaacataa tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca
     3541 atctatgcaa agaaaagtaa atcgcagtga tagcgattat cattacggta aagaaaaata
     3601 caagcggaat gtttataaaa tgatgttgaa atgtaaacaa agagaggtaa tgccagggga
     3661 tataaacgat tattccaggt acaaagatgg gattgtttac aacgagccat gtgttcttgc
     3721 cggatgccgc gtaaacgccg tatccagccg acaaaaccgt cctgactcaa tacatcaaaa
     3781 cttccatgta aaactgataa gcgggtgtat caagcaattt cgtccatcct cgattatcaa
     3841 atctaacttt ttgcccggaa cataataaca gctcgggtgt tggtggcgtc aaaatcgctg
     3901 aagcgttccc cgaaggccgg gacagcaggg ggccgcggcg attggccgcc ccctgcgcgc
     3961 tccctgcacc attcaattta tatggcgagg cacattaagg gagcgcaacc cgctgctgaa
     4021 gccaaacaaa accgccgcag gctaagtccc ttagctttcc tgcaaccgtg acggcggagc
     4081 cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt
     4141 acccagcaaa ctaaacacta caatcccgat actgccgaag atgaagcccg gaataatttc
     4201 gtagagtccc agccagccga actgcttcca gacgataacc gtcagcgcac cgatgatcat
     4261 ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg
     4321 accaaacgcc gcgccaaagc ctgcccacgc gtagctcact aagcccagca cgcggttttc
     4381 cgggtttgcc gccagcgcaa tcgccaccag cgccaccacc agcaccatca cacgccctac
     4441 ccacaccagc tctttctggc tggcatgttt acgcagaaac gctttgtata aatcttcggt
     4501 aatcgcactg gagcacacca gcagctggca acttaaggtt gacattaccg ccgccagaat
     4561 tgccgacagc agaatcccgg caatccacgg gttaaacaga atttgcgcca gttcgataaa
     4621 cacgcgctca gcattctggt ttaccgcacc tgctaccgcc gggtgctcat taaagtaagc
     4681 aataccaaag aagccgacag ctaccgcccc tgccaggcag aggatcatcc aggtcatact
     4741 gatacgacgc gcatggacaa tgctgtggtg agagtctgcc gccataaaac gcgccaggat
     4801 gtgcggctgc ccgaagtaac ccagccccca acccatcagt gagataatgg cgacaaagtt
     4861 cagccctttg agcatatcaa cgttttcgat gctcttttgt ttgatcactt ccagcgagtc
     4921 accaaagcca ccgacactga taatgacgat aaccggcgtc aggatcaggg caaaaatcat
     4981 caggctggcc tgtacagtgt cagtccagct caccgcgagg aaaccgccaa taaaggtgta
     5041 aaggatcgtc gccgcagctc ccgcccacag agccgtttcg tagctcatgc caaaggtact
     5101 ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa
     5161 aatcaccagc gcggagataa tgcgcaaaat gcggctttta tcttcaaagc gcccggtgaa
     5221 ataatccggc aatgttaagg cgttattgtt gtattcggta tgcacacgca accgccccgc
     5281 caccagcttc cagttgatcc acgcgcctaa tgtcaggcca atggcgatcc aactttcgga
     5341 aatcccggaa agaaaaaccg cgcccggcaa ccccattaac agccagccgc tcatatccga
     5401 cgcacccgcc gataatgccg tcacgaatgg cccaaggcta cgaccgccca gaatatagtc
     5461 gtcaaagttt ttcgttgatc gccaggcgat aaacccaatc aatatcatgc caaagatata
     5521 gacacaaaat gtcaccaaca tcggtgtgct aatagccatc taaagtctcc aaaaaattat
     5581 tatcggcaat gtcgaaactt gccgttatat ctgccaccgg aacggggtaa cagagtttat
     5641 gttttaccag ggcgaccgta tcctgccgga agcgctggtt attcacaatc gatttaacac
     5701 accatttaca ttaaatttta gtgctcagcg acactatttt tcatcaggtt gcactctctc
     5761 acattttttg cggttgcacc tttcaaaaat gttaactgcc gcagagaaaa agtctgagtt
     5821 atttttttaa tccctgtcat atcgatttct tttattaaca tttcattcat ttttaagctt
     5881 gctacgcatg tcacatttaa catggttgca caaagttgca acatcatgga tatttcacga
     5941 taacgttaag ttgcaccttt cagaacaaca ggagtaatgg catgggaacc accaccatgg
     6001 gggttaagct ggacgacgcg acgcgtgagc gtattaagtc tgccgcgaca cgtatcgatc
     6061 gcacgccaca ctggttaatt aagcaggcga ttttttctta tctcgaacaa ctggaaaaca
     6121 gcgatactct gccggagcta cctgcgctgc tttctggcgc tgccaatgag agcgatgaag
     6181 caccgactcc ggcagaggaa ccacaccagc cattcctcga ctttgccgag caaattttgc
     6241 cccagtcggt ttcccgcgcc gcgatcacag cggcctatcg ccgcccggaa accgaagcgg
     6301 tttctatgct gctggaacaa gcccgcctgc cgcagccagt tgctgaacag gcgcacaaac
     6361 tggcgtatca actggccgat aaactgcgta atcaaaaaaa cgccagtggt cgcgcaggta
     6421 tggttcaggg gttattgcag gagttttcgt tgtcatcgca ggaaggcgtg gcgctgatgt
     6481 gtctggcgga agcgttgttg cgtattcccg acaaagccac ccgtgatgcg ttaattcgcg
     6541 acaaaatcag caacggtaac tggcaatcac acattggtcg tagcccgtct ttgtttgtta
     6601 atgccgccac ctgggggctg ctgtttactg gcaaactggt ttccacccat aacgaagcca
     6661 gcctctcccg ctcgctgaac cgcattatcg gtaaaagcgg tgaaccgctg atccgcaaag
     6721 gtgtggatat ggcgatgcgc ctgatgggcg agcagttcgt cactggcgaa accatcgcgg
     6781 aagcgttagc caatgcccgc aagctggaag agaaaggttt ccgttactct tacgatatgc
     6841 ttggcgaagc tgcactgact gccgcagatg cacaagcgta tatggtttcc tatcagcagg
     6901 cgattcacgc cattggtaaa gcgtccaatg gtcgtggcat ctatgaaggt ccgggcattt
     6961 caatcaagct gtcggcgctg catccgcgtt acagccgcgc ccagtatgac cgggtaatgg
     7021 aagagcttta cccacgtctg aaatcactca ccctgctggc gcgtcagtac gatattggta
     7081 tcaacattga cgccgaagag gccgatcgtc tggagatctc cctcgatctg ctggaaaaac
     7141 tctgtttcga gcctgagctg gcaggctgga acggtatcgg ttttgttatt caggcttatc
     7201 aaaaacgctg cccactggtg attgattacc tgattgatct cgccacccgc agccgtcgcc
     7261 gtctgatgat tcgcctggtg aaaggcgcgt actgggatag tgaaatcaag cgtgcgcaga
     7321 tggacggcct tgaaggctat ccggtttata cccgcaaggt gtataccgac gtttcatatc
     7381 tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat ctacccgcag ttcgcgacgc
     7441 acaacgccca tacgctggca gcgatttatc aactggcagg tcagaactac tatccgggtc
     7501 agtacgagtt ccagtgcctg catggtatgg gcgagccact gtatgagcag gtcaccggga
     7561 aagttgccga cggcaaactt aaccgtccgt gtcgtattta tgctccggtc ggtacccatg
     7621 aaacgctgtt ggcgtatctg gtgcgtcgcc tgctggaaaa cggtgctaac acctcgtttg
     7681 ttaaccgtat tgccgacacc tctttgccac tggatgaact ggtcgccgat ccggtcactg
     7741 ctgtagaaaa actggcgcaa caggaagggc aaactggatt accgcatccg aaaattcccc
     7801 tgccgcgcga tctttacggt cacgggcgcg acaactcggc agggctggat ctcgctaacg
     7861 aacaccggct ggcctcgctc tcttctgccc tgctcaatag tgcactgcaa aaatggcagg
     7921 ccttgccaat gctggaacaa ccggtagcgg caggtgagat gtcgcccgtt attaaccctg
     7981 cggaaccgaa agatattgtg ggctttgtgc gtgaagccac gccgcgtgag gtagaacagg
     8041 cgctggaaag tgcggttaat aacgcgccaa tctggtttgc cactcctccg gttgaacgcg
     8101 cggcgatttt gcatcgcgct gccgtgctaa tggaaagcca gatgcagcaa ctgattggta
     8161 ttctggtgcg tgaagccgga aaaaccttca gtaacgccat tgccgaagtg cgcgaagcgg
     8221 tcgattttct ccactactat gccggacagg tgcgggatga tttcgctaac gaaacccacc
     8281 gtccattagg gcctgtggtg tgtatcagtc cgtggaactt cccgctggct attttcaccg
     8341 ggcagattgc tgccgcactg gcggcaggta acagcgtgct ggcaaaaccg gcagaacaaa
     8401 cgccgctgat tgccgcgcaa gggatcgcca ttttgctgga agcgggtgta ccgccaggcg
     8461 tggtgcagtt gctgccaggt cagggggaaa ccgtgggcgc gcaactgacg ggtgatgatc
     8521 gcgtacgcgg ggtgatgttt accggttcaa ccgaagtcgc tacgttactg cagcgcaata
     8581 tcgccagccg cctggacgct cagggtcgcc ctattccgct catcgctgaa accggcggca
     8641 tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca ggtcgtcgtg gatgtactgg
     8701 cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcgct gcgcgtgctg tgcctgcaag
     8761 atgagattgc cgaccacacg ttgaaaatgc tgcgcggcgc aatggccgaa tgccggatgg
     8821 gtaatccggg tcgcctgacc accgatatcg gtccggtgat tgatagcgaa gcgaaagcca
     8881 atattgagcg ccatattcag accatgcgta gcaaaggccg tccagtgttc caggcggtgc
     8941 gggaaaacag tgaagatgcc cgtgaatggc aaagcggcac ctttgtcgcc ccgacgctga
     9001 tcgaactgga tgactttgcc gaattgcaaa aagaggtctt tggtccggtg ctgcatgtgg
     9061 tgcgttataa ccgtaatcag ctgccggagc tgaccgagca gattaacgct tccggctatg
     9121 gtctgacgct tggcgtccat acacgtattg atgaaaccat cgcccaggtc actggctcgg
     9181 cccatgtcgg taacctgtac gttaaccgta atatggtggg cgcagtggtt ggtgtgcagc
     9241 cgttcggcgg cgaagggttg tccggtaccg ggccgaaagc aggcggtccg ctctatctct
     9301 accgtctgct ggcgaatcgc ccggaaagtg cgctggcagt aacgctcgcg cgtcaggatg
     9361 cagagtatcc ggtcgatgcg cagttgaaag ccgcattgac tcagccgcta aatgcactgc
     9421 gggaatgggc agcaaatcgt ccagaattgc aggcgttatg tacgcaatat ggcgagctgg
     9481 cgcaggcagg aacacaacga ttgctgccag ggccgacggg tgaacgcaac acctggacgc
     9541 tgctgccgcg tgagcgcgtg ttgtgtattg ccgatgatga gcaggatgcg ctgactcagc
     9601 tcgccgccgt gctggcggtg ggcagccagg tactgtggcc ggatgacgcg ctgcatcgtc
     9661 agctagtaaa ggcattgcca tcggcagtca gcgaacgtat tcaactggcg aaagcggaaa
     9721 atataaccgc tcaacccttt gatgcggtga tcttccacgg tgattcggat cagcttcgcg
     9781 cattgtgtga agccgttgct gcgcgggatg gcgcaattgt ttcggtgcag ggttttgccc
     9841 gtggcgaaag caatatcctt ctggaacggc tgtatatcga acgttcgctg agtgtgaata
     9901 ccgctgccgc tggcggtaac gccagtttaa tgacgatcgg ttaaacaggt agccggagga
     9961 tgttacaacc tcctccggca tctttaacgt ggtcgaatcc cctcaataat aatccgctgc
    10021 acgttttcaa ccgtttgatt gaaaaatacc tcatcgcgca acgtcgcgcc tgtcaccgcc
    10081 tccacctgag gggcaaaatc ggcgtaatgt tgagtggaag cccaaatcat aaaaatcaaa
    10141 tgctgcggat caatcggcgc gagtttgccg cttttgaccc aaccggcaat cagcgccgat
    10201 ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat ccattaacag cggcgcgcct
    10261 gccagcatct ccatacagaa caggcgcgaa gcctgcggat aatcgcggga gacttccagc
    10321 ttcagacgga tgtactcttt gatcgccgcc agcggggcga aatcttctcg aaacgctttt
    10381 aacggtgcca gccagatatc gagaatctgc cgcagcacgg caatatacag cgcctctttt
    10441 gacggaaagt aatacagcag attggttttt gaaacacccg ccaactctgc gatctgctcc
    10501 agccttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca gtgctgcgct aagaatcgct
    10561 tttttcttcg cgcttactgc gcgcgaacgt ttaccctttg ttttcactgc gccttgcgtc
    10621 atgcgctctc ccctcttgta gattggcaac aggatagcaa aacccccgcc cggcctcgac
    10681 cttctgcact ctcatcgcgc acagatgcat gttttatgtg caactgtttt gaccgtttag
    10741 tccacttttt accagatatt ttagataacg cattgttaac atattaacaa cattaacttt
    10801 ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg gctgaaaagg atatgcagga
    10861 tgcagcgccc cgtctgactt tcactttacg agacgaagag aggttgatga tgaaaattgg
    10921 cgtattcgta cctattggca acaacggctg gctcatttcg acccacgcgc cgcagtacat
    10981 gccgaccttt gaactgaata aagccattgt gcaaaaagcg gagcactacc atttcgattt
    11041 cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa actgagttct gggatcacaa
    11101 ccttgagtcg ttcaccttga tggcggggct ggcggccgtg acctcgcgca ttcagattta
    11161 cgccactgct gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat
    11221 cgactccatc tctggcgggc gttttggcgt caacctcgtg actggctggc aaaagcccga
    11281 gtatgagcag atgggtatct ggcctggcga tgactatttc tcccgtcgtt acgactatct
    11341 caccgaatat gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg attttaaagg
    11401 cgattttttc accatgaatg attgtcgcgt cagtccgcaa ccgagtgtcc ccatgaaagt
    11461 gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc gcccagtatg ccgatttcaa
    11521 cttctgtttc ggcaaaggcg taaatacgcc cacggctttc gccccgaccg ctgcgcggat
    11581 gaaacaggcc gcagagcaaa ccgggcgcga cgttggttct tatgtgttgt ttatggtgat
    11641 tgccgatgaa accgacgatg ccgctcgcgc caaatgggaa cactacaaag cgggcgcgga
    11701 tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa gatacccgct ccggtactga
    11761 taccaacgtc cgtcagatgg ccgatcccac ttcggcggta aacatcaata tggggacgtt
    11821 agtcggttct tacgccagtg tcgcgcgcat gttagatgaa gtcgcaagcg tgcctggtgc
    11881 cgaaggcgtg ctgttgacct tcgatgattt tctgtcggga atcgaaacct tcggcgagcg
    11941 cattcaacca ctgatgcagt gccgcgccca tctacctgcg ctgactcagg aggtggcatg
    12001 atgacaacct taaccgctcg accggaagcc attaccttcg atccgcagca aagtgcgctg
    12061 atcgtggtgg atatgcaaaa cgcctatgcc acgccaggcg gctacttaga tctcgccggg
    12121 tttgatgtct caaccactcg cccggtcatt gccaacattc aaaccgccgt gaccgcagcg
    12181 cgagcggcag ggatgctgat catctggttt caaaatggct gggatgaaca gtatgtcgaa
    12241 gctggcggcc ccggctcacc gaattttcat aaatcgaacg ccctgaaaac catgcgtaag
    12301 cagccgcagc tgcaggggaa attgctggcg aaaggctcct gggattatca actggtggat
    12361 gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc cgcgctacag cggtttcttc
    12421 aatacaccgc tggacagcat tttgcgcagc cgtggaatac gccatctggt tttcaccggt
    12481 atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg gcttttttct ggagtatttc
    12541 ggcgtggtgc tggaagacgc aacacaccag gcggggccgg aatttgcaca gaaagccgcg
    12601 ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg tcgaaacgtt ctgcgacgcg
    12661 ctttctccca catcctttgc tcgtatcgct taaggagttt aacgatgcca aaatccgtaa
    12721 ttattcccgc tggcagcagc gcaccgctgg cccccttcgt tcccggcacg ctggctgatg
    12781 gcgtggtgta tgtctccggt acgctggctt ttgatcaaca taataacgtg ctgtttgccg
    12841 atgacccaaa ggcgcaaacc cgccacgttc tggaaattat ccgcaaggtg atcgagacgg
    12901 cgggtggcac gatggcggat gtgaccttca acagcatctt tattaccgac tggaaaaatt
    12961 acgccgcgat taacgaaatc tacgccgagt tttttccggg tgataaaccg gcgcgattct
    13021 gcattcagtg cggactggta aaacctgacg cgctggtgga aatcgccaca attgcgcata
    13081 tcgccaagtg aggccgcgat gaaactttca ctctcacctc ccccttatgc tgatgcgccc
    13141 gtagtggtgt tgatttcggg tcttggcggt agcggcagtt actggttacc gcaactggcg
    13201 gtgctggagc aggagtatca ggtagtctgt tacgaccagc gcggcaccgg caataatccc
    13261 gacacgctgg cagaagatta cagtatcgcc cagatggcag cggaactgca tcaggcgctg
    13321 gtagccgcag ggattgagca ttacgcggtg gtcggccatg cgctcggtgc gctggtggga
    13381 atgcagctgg cgctggatta tcccgcgtcg gtaactgtgc tgatcagcgt taacggctgg
    13441 ctacgaataa acgcccatac gcgccgctgt tttcaggttc gcgaacgatt actgtatagc
    13501 ggcggcgcgc aggcatgggt ggaagcgcag ccgttgttcc tctatcccgc cgactggatg
    13561 gcggcccgcg cacctcgcct ggaggcagaa gacgcgctgg cactggcgca ttttcagggc
    13621 aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg ctgactttag tcaccatgcg
    13681 gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg atgatctgct ggtgccatca
    13741 gcatgttcca gtgagcttca tgccgccctg cccgatagcc agaaaatggt gatgccctat
    13801 ggcggacacg cctgcaacgt gaccgatccc gaaacgttta atgctctgtt actcaacggg
    13861 cttgccagcc tgttacatca ccgtgaagcc gccctgtaag gaattgctat gaacgaagcc
    13921 gttagcccag gtgcgcttag caccttgttc accgatgccc gcactcacaa cggctggcgg
    13981 gagacacccg tcagcgatga gacgttacgg gagatttatg ccctgatgaa atgggggccg
    14041 acatcagcta actgttctcc ggcacggatc gtgtttatcc gcacggcaga aggaaaagaa
    14101 cgtctgcgcc cggcactttc cagcggcaat ctgcaaaaaa ccctgaccgc gcccgtcacc
    14161 gctatcgtcg cctgggacag tgaattttat gaacggttac cgcaactgtt tcctcacggc
    14221 gatgcccgca gttggtttac ctccagccca caacttgccg aagaaacagc gtttcgcaac
    14281 agttccatgc aggcggccta tctgatcgtc gcctgccgtg cgctgggact ggataccggc
    14341 ccgatgtcgg gttttgaccg tcaacacgtt gacgacgcct tttttgcggg cagcacgctg
    14401 aagagcaatc tgctgattaa tatcggctat ggcgatagca gcaaactttt tgcgcgcctg
    14461 ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag gagcaaacca tgaacattgt
    14521 cgatcaacaa acctttcgcg atgcgatgtc ctgtatgggc gcggcagtca atatcatcac
    14581 cacggatggt ccagccgggc gcgccgggtt caccgccagc gcggtctgca gcgtgaccga
    14641 cacgccgcct acattactgg tgtgcctgaa tcgtggggcg tccgtctggc cggtattcaa
    14701 tgaaaatcga acgctgtgtg taaatacact gagcgccggg caggagccgc tttcaaatct
    14761 ttttggcggc aaaacgccca tggaacaccg ctttgccgcc gcccgctggc agacgggtgt
    14821 gaccggatgc ccgcaactgg aagaggctct ggtttcgttt gactgccgta tcagccaggt
    14881 ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc gaagcgattc atcgtcacgc
    14941 cacaccctac gggctggtgt ggttcgatcg cagttatcac gcgctgatgc gccctgcttg
    15001 ttaacctcct aaaggagaca gctgatggca atgttcggtt ttcctcactg gcagttaaaa
    15061 tcgacctcta cagagagcgg cgtggtcgcg ccgtatgaac gattgccgtt tgctcagacg
    15121 gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg cgacggtgtt aatgccgatt
    15181 ctgatggggc tggatcccaa tctttccatt ttaatgtcgg ggatcggcac gttactgttt
    15241 ttctttatta ccggcgggcg cgtccccagt tatctcggtt ccagcgcggc ttttgtcggc
    15301 gtggtgattg ccgcgacggg ctttaacggt cagggcatta acccgaatat cagcattgcg
    15361 ctgggcggga ttatcgcctg tgggctggtt tataccgtca ttggcctggt ggtgatgaaa
    15421 attggtacac gctggattga acgactaatg ccgccggtgg tgacgggtgc ggtggtgatg
    15481 gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg tatcggcttc ggcctttgat
    15541 agctggatgg cggtaatgac ggtgctgtgt attgggctgg tggcggtgtt tacccgtggc
    15601 atgatccagc ggttattgat tctcgtaggt ttaattgtcg cctgtctgct gtatggcgtg
    15661 atgaccaacc ttttagggct gggaaaagcc gtcgatttta ctctcgtcag ccatgctgcc
    15721 tggttcggtt tgccacattt ttccactcct gcttttaata gtcaggcaat gatgctgatt
    15781 gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc atctcaaggc ggtcgccggg
    15841 atgaccggac gcaatatgga cccgtatatg gggcgggcat tcgtcggtga tgggctggcg
    15901 acgatgcttt ctggttctgt cggtggcagc ggcgtaacca cctacgccga gaatatcggt
    15961 gtaatggccg tgacaaaagt ctactcgacg ctggtgtttg tcgctgctgc cgtcatcgcc
    16021 atgctgttgg gcttttcacc gaaatttggc gcgctgattc ataccattcc ggccgcggtg
    16081 attggtggtg catcaattgt tgtgttcggg ctgattgccg tcgcaggcgc aaggatatgg
    16141 gtacaaaacc gtgtcgattt aagccagaac ggtaatttga ttatggtcgc agtaacctta
    16201 gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt ttacgttggg agggattggt
    16261 acagcaacct ttggcgcgat tttactcaat gcgttgttaa gcagaaagtt ggttgacgtt
    16321 ccaccgccag aagttgttca tcaggagcca taaacagaaa accgccggga ttaccggcgg
    16381 tgatgacttt ttgacgagcc taagaaaaaa atgcattcgt cattgattag cccggctagt
    16441 tgtcgctttt gccgtgactg ctcttgccac cttttttacc tgcttcagat gcgcgctgcg
    16501 ggtcattctt gaaattaccc ccgctgtgct gtccaccttt tttacctgct tctgatgctc
    16561 tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc
    16621 gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc tgccatattt
    16681 tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt ggtaatatcc
    16741 tgcaacagct tattagcgag aatagatatc atctccaata attatccata agccgctctt
    16801 atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg
    16861 tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt
    16921 atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga
    16981 aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg
    17041 cgcagtcgct gagggtgcaa gcaaagtcga tggcgcagaa gttgtcgtta agcgcgtacc
    17101 ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt
    17161 tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt
    17221 tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc
    17281 cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca
    17341 ggaacaaact attacttcaa cctggacgac ccttgcgcat cacggcatgg taattgtccc
    17401 cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta
    17461 cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc
    17521 tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc
    17581 ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg
    17641 gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac
    17701 gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc
    17761 tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac
    17821 gtttaaccag cgactccccc acttctcgcg ggggagtttt ctgttatttc accgcctcat
    17881 tcaacacgct atcaaactta tccattgggc agaaaccgtt ggcatctatc gggcaaccgc
    17941 ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta
    18001 actgttccgc actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt
    18061 catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca
    18121 actgatacgg tttgaaatcc agcgccgtta acagagaggc aatgttggag tcgtgcccaa
    18181 ccaacactgt aattttcggt gcgctggtgc gatcggtgac cagagctttg tcgatataac
    18241 tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt
    18301 agccgttttt aagcttcgac aacaccttcc actgctggtc agatttgatt tctccccagg
    18361 ccacctgatc catcggaaaa ccttcgtaat attgcaaagt aaacgcatct accagcgagt
    18421 tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat
    18481 ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt
    18541 taacgatttt ttccagtagt tggtagctgt cggtaagctg gagtttgctg agctctttct
    18601 ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc accgggttaa
    18661 aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca caccccggga
    18721 atgcgccggt aataaagaac tgtgcggtcg caacggtacg ttgcagacta ttggcatagg
    18781 cataaacggt gtccggcggc gggcattccc ccgatttcac catcccctgc tgtgccagcc
    18841 attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc
    18901 caccggggac gtcccattcg ggccatttat tcggcgtaga ctgctccagc acactgccat
    18961 tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat
    19021 agccttccgg tacggtttgt gcctgagcgt ttgaagcgag taaaactatc cctgccacag
    19081 ctgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat cactaaagag
    19141 tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct
    19201 gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc
    19261 ccctgcctcg cgtacgccgc tgataatttc acctgtggct aaacagaata tctgcggcgt
    19321 taccaataca gagacaatat ttaccaataa aaagtgtgag actaataaca atggttttta
    19381 gcatatgcct ttgccattaa attgcataac gatttaaata gtcgagttgc gcattcattt
    19441 ccttattttt taacttataa gcctgagcaa aaagttcagg agggaggtaa acatcataat
    19501 gattgccagc gtttattaaa taaccatact caaggtcgtc atctgttgaa aaggattc
//
LOCUS       DAPPNJ010000078        19466 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000078 DAPPNJ010000000
VERSION     DAPPNJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.078"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            10..912
                     /locus_tag="RPM73_002285"
     CDS             10..912
                     /locus_tag="RPM73_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520639.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1059..2279
                     /locus_tag="RPM73_002286"
     CDS             1059..2279
                     /locus_tag="RPM73_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDX6520640.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2252..2881
                     /locus_tag="RPM73_002287"
     CDS             2252..2881
                     /locus_tag="RPM73_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDX6520641.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2882..4042)
                     /gene="ybdL"
                     /locus_tag="RPM73_002288"
     CDS             complement(2882..4042)
                     /gene="ybdL"
                     /locus_tag="RPM73_002288"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDX6520642.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4151..5239
                     /gene="hcxA"
                     /locus_tag="RPM73_002289"
     CDS             4151..5239
                     /gene="hcxA"
                     /locus_tag="RPM73_002289"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDX6520643.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5249..5446)
                     /locus_tag="RPM73_002290"
     CDS             complement(5249..5446)
                     /locus_tag="RPM73_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDX6520644.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5622..7727)
                     /gene="cstA"
                     /locus_tag="RPM73_002291"
     CDS             complement(5622..7727)
                     /gene="cstA"
                     /locus_tag="RPM73_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDX6520645.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7908..8321)
                     /gene="entH"
                     /locus_tag="RPM73_002292"
     CDS             complement(7908..8321)
                     /gene="entH"
                     /locus_tag="RPM73_002292"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDX6520646.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8324..9070)
                     /gene="entA"
                     /locus_tag="RPM73_002293"
     CDS             complement(8324..9070)
                     /gene="entA"
                     /locus_tag="RPM73_002293"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDX6520647.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9070..9927)
                     /gene="entB"
                     /locus_tag="RPM73_002294"
     CDS             complement(9070..9927)
                     /gene="entB"
                     /locus_tag="RPM73_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDX6520648.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9941..11551)
                     /gene="entE"
                     /locus_tag="RPM73_002295"
     CDS             complement(9941..11551)
                     /gene="entE"
                     /locus_tag="RPM73_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDX6520649.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11561..12736)
                     /gene="entC"
                     /locus_tag="RPM73_002296"
     CDS             complement(11561..12736)
                     /gene="entC"
                     /locus_tag="RPM73_002296"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDX6520650.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13111..14067
                     /gene="fepB"
                     /locus_tag="RPM73_002297"
     CDS             13111..14067
                     /gene="fepB"
                     /locus_tag="RPM73_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDX6520651.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(14071..15321)
                     /gene="entS"
                     /locus_tag="RPM73_002298"
     CDS             complement(14071..15321)
                     /gene="entS"
                     /locus_tag="RPM73_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDX6520652.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15432..16436
                     /gene="fepD"
                     /locus_tag="RPM73_002299"
     CDS             15432..16436
                     /gene="fepD"
                     /locus_tag="RPM73_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDX6520653.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16433..17425
                     /gene="fepG"
                     /locus_tag="RPM73_002300"
     CDS             16433..17425
                     /gene="fepG"
                     /locus_tag="RPM73_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDX6520654.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17422..18237
                     /gene="fepC"
                     /locus_tag="RPM73_002301"
     CDS             17422..18237
                     /gene="fepC"
                     /locus_tag="RPM73_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDX6520655.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18234..19367)
                     /gene="fepE"
                     /locus_tag="RPM73_002302"
     CDS             complement(18234..19367)
                     /gene="fepE"
                     /locus_tag="RPM73_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDX6520656.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAIMADHLV"
BASE COUNT         4640 a         5444 c         4953 g         4429 t
ORIGIN      
        1 gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaacct tcttgtcata
       61 tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact atatatcacc
      121 ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga ccctttattc
      181 attcgctcag gaaaaggtat cgcgccgacg acaacagggt tgaatctgca tcaccatctt
      241 gagaaaaacc tcaaaggcct tgaacaaaca attaatatag tcaataagtc cgaactaaag
      301 aaaaacttta ttatctacgg ccctcagctc atttcttgct ctaataatag tatgctgatc
      361 cgctgcctac ggcaagatgc ctctgtcgag attgagtgcc atgatatcct catgtcagca
      421 gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca
      481 gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa ttcgcaatac tctaatttgt
      541 agtaataagc atccgcgaat tactgacaat agcacttatg aacaaattat ggcagaagag
      601 ttcacccaat taatatccaa atcagcagga gttgatgata ttcaaatgga aatagacgag
      661 aagtttatga atcgcaaaat ttcatttcgt ggttcctctt tgctgacaat cattaatagc
      721 attgcagtta cagatttact gggaatagtc ccctatgaac tttataattc ctaccgtgac
      781 tttcttaatt taaaagagat aaaactagaa catccgttac cttcaataaa gctttacatt
      841 tcctataata aatcttcttt aaataatttg gtgttctccc gatttattga ccgtctaaat
      901 gagagttttt gatatataac tacagacagc tactataact ttcatctatt tattcacagc
      961 gcgaatgaaa aataaaacac tctgctttgc agtgtttact ttataattaa cagacttatc
     1021 tcactgatca ccctgtaacg ttcagagagc gtcttcagat gtctatttat aaaattcctc
     1081 ttccgctcaa tattctggaa gctgcacgag agcgtattac ctggacactc aacaccctac
     1141 cccgcgtttg tgtctcattt tctggcggca aagactctgg tttaatgctg catctgacag
     1201 ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac tgggaggcgc
     1261 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
     1321 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
     1381 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
     1441 taaccgatcc tgactttttc tgcttttacc agcctggcat gaccttcgaa caatttgtac
     1501 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
     1561 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
     1621 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
     1681 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaagt ctctgcaacc
     1741 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
     1801 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
     1861 gggcagctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
     1921 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
     1981 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
     2041 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
     2101 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
     2161 acgattactg gtgtcgagca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
     2221 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     2281 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     2341 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
     2401 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
     2461 acccgaaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc aacccattgt
     2521 ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc gccatgaaat
     2581 tggtaaaggc tcatcatcat taaaattacg cttaaaaggt tatttgccgg tgacctgtct
     2641 ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg ctcgtggacg
     2701 ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg gatgggacga
     2761 taataaaatt ggcaaagagc taggaatgga cagcgacgaa gtgctgcgcc tgaagcaaat
     2821 taacggcctg caagagctgt ttgccgaccg tcaatattcc cgcgcctgga cggttaaata
     2881 actaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc ttggcaaaac
     2941 agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc ggaatcgccg
     3001 ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg gtagaaacct
     3061 cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc agccggcttt
     3121 catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt aacgcaagat
     3181 aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg gtattcaccg
     3241 aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct ggcgcaacac
     3301 aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa accgccactg
     3361 cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt gaaaagttga
     3421 tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc gcctgccaca
     3481 aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcgga gtgttgagga
     3541 tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca acgcgaaaat
     3601 gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggagt
     3661 aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa
     3721 tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg
     3781 gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc
     3841 cggtcatggg cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta
     3901 aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct
     3961 gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg
     4021 gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact
     4081 ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg
     4141 acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc
     4201 acatccagga agtttcaatc acctgcacga ttttttcact gatgaacaac tttctcgcgc
     4261 ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt
     4321 tggacttcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca
     4381 gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc
     4441 actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccgtttg ttgccgttcc
     4501 gacgatcgcc gccacctgcg ccgcctggac accgctctcc gtctggtata acgatgccgg
     4561 acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga
     4621 gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa
     4681 atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcgact
     4741 ggggatcaat aatgcgcagg ccattcgcga cgtattgtta aacagtagcg aacaggcgct
     4801 gagcgatcag caaaatcaac agttaacgca atcattttgc gatgtggtgg atgctattat
     4861 tgctggtggt gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca
     4921 tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag aaatttctcc acggcaccaa
     4981 agtcgcttat ggcattctgg tacaaagcgc cttgctgggt caggatgatg tgctggcgca
     5041 attaaccggg gcgtatcagc gttttcatct gccgaccacg ctggcggagc tggaagtgga
     5101 tatcaataat catgcggaga tcgacaaagt gattacccac accctgcgtc cggtggaatc
     5161 cattcattac ctgccggtca cgctgacacc agatacgtta cgtgcagcgt tcgaaaaagt
     5221 ggaatcgttt aaagcctgac gtacatattt agcagcagcg cgcgccgcct tttccaccgt
     5281 agcgcgcgtc ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat
     5341 ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg
     5401 ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta
     5461 accatgttgc tgaaagagtg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatgct
     5521 atcaacgcgc tcggtcagtc ccctcgcccc accggggaga gggtgagggg gaaccctcgg
     5581 cgccatgcca acattcgcca acattccccc tcactctgac tttagtgtgc gccttttgcc
     5641 tgcgccacga tctcctcgac attttccggc attggctcat acggcgtttc tttcgccgtt
     5701 ggcttcggct ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc cagaaccacc
     5761 acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa caccagttgc
     5821 gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac
     5881 ttgttagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt ctgccagcct
     5941 gcggtcaggg tacaaatcag tagccaggcc gttggtacca gcgccaccca ggcgtaacgt
     6001 tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc tgccagcatc
     6061 tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg atcgaccacg
     6121 ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ccgttgccag caggttagca
     6181 ggcagtgaat cggtccgctt caggccagga gacaccacgc ccagcagatc ctgcaacata
     6241 aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc ttcaaacaga
     6301 atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc gtgcaggatg
     6361 taagccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat cgactgttca
     6421 cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc ccagctactc
     6481 accacctgcg cggcagaagc gactacatcc gccgtccctg ccggagccag caccgccatt
     6541 gggctgttca tggcaaaata cacgcccgga tcgatgatac aggcagaaac cagcgccata
     6601 atcgccacga aggattccat caacatccca ccgtagccga taaagcacgc ctgcccttcg
     6661 ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc agacaccgca
     6721 ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac cgggccagtg
     6781 ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat caaaatgcct
     6841 accgccagac cgacgatagt cccgattttc aggaaggtcg agagatagtc acgcggggcc
     6901 agcagcaacc acaccggcag caccgccgcc acaaaaccgt agcccaccag catccaggtc
     6961 agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc cacccagccg
     7021 ccagagataa tggcgaaaat caggaacacc aggccaatca ctgacacttc gccaatgcgg
     7081 cccggacgca gatagcgcag gtagattccc ataaaaatcg ccagcggaat ggtgaacgca
     7141 acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat cgccagtact
     7201 gcaaggataa tgaccatgat cataaagcag gctaccagcg caatcacccc ggcagttggc
     7261 cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt agaaacaaac
     7321 agcaccatga aatcctgcac cgcaccagcg agaaccaccc cggcgagcag ccagatcatc
     7381 cctggcaggt aacccatttg cgccgccagt accggcccaa ccaacggacc tgctccggca
     7441 atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata gtccagcccg
     7501 tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac atttttggcg
     7561 atatacagcc cataaaaacg gtaagcgatc agatagatac agaccgacgc caccacaatc
     7621 cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc aaatgctccc
     7681 attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt tgttatccgt
     7741 gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtacg atttaacaac
     7801 acggtaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt taactcgatc
     7861 actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca tcccaaaact
     7921 gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac gacgatttcc
     7981 cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac cttgccttca
     8041 gacaccgggc gatgatgggt ggcgttaagt tccgtaccca ccacacactg cccgtcacgg
     8101 gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga cgcgccgcca
     8161 tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc ttccagcaca
     8221 tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt atcgctggtg
     8281 gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc cccagcgttg
     8341 agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca gaggcgagga
     8401 acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga atgccgagtt
     8461 taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc acccacagcg
     8521 tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc acgccgctac
     8581 ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc gatgcgccat
     8641 aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg acaatcgccc
     8701 cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac gcaccgccga
     8761 cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc gcgcccatgc
     8821 gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct aacagtcgct
     8881 gacatacctg cgccacctgc ccggcgtcgg caacatccat tacttcggtc gcaaagggat
     8941 attgctcctg agtgaacgct tgatcaaaac ctgtaacttt cgctcccgcc tcaacaaacg
     9001 ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca tttttaccgc
     9061 tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga tggtcgggtt
     9121 tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg ccgccagcgc
     9181 catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga acggttcatc
     9241 ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg cggggacagg
     9301 tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca catatttcag
     9361 cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca taaacggttt
     9421 aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg catatacccc
     9481 ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg gagaacgatg
     9541 aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg tcaggcgatc
     9601 caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat cattcaacag
     9661 cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaaccg ggatattgtg
     9721 ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca tcatcgggca
     9781 gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca acgcggcacg
     9841 ttgcggttca aaggcccagt cgactttatt cttagggata tcgtgagact ccggcagtgc
     9901 gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat gcgcgtgacg
     9961 ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc ggaagtgaat
    10021 ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca cgcaggaaac
    10081 gacgcacctg caccgcgcgc agcggttctt tcaccaccag ataagcgcag cttttttcgc
    10141 ccatcagctc atcttccatg ctcaccagtg cggcgtagat caccgccggg tggcgcagca
    10201 gcaggttttc gatctcttcg gcagcgatct tctcaccgcc acggttgatc tgatctttct
    10261 cgcgcccctg cacggtgatg tacccctctg gatcaataga gatcagatcg ccggaacagt
    10321 aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa tagccacgga
    10381 aggtgtacgg accgcgggtc atcaggcgtc caacttcccc ttgcggcagt ggatttcctt
    10441 cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg gtatggataa
    10501 ttttctccgc gctatcatca agtcgggtgt agttcaccag cccttccgcc atgccaaaca
    10561 cctgctgcaa ctgacagcca atctcagcgg gaatacgcgc cgcaagggtg gcagaaagac
    10621 gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt tcgccttcga
    10681 tcagcgcctg caaccacagg ctgactgcgg gtggcaccag cgcggtaacg ttaacctgat
    10741 gtttttcaat caatgggaaa cagagcgtgg cgctgggatc ggccgccaga acaaccgttc
    10801 ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta tgagccgccg
    10861 ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca aatctcgacg ctacgacgca
    10921 cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg gtggtgccgc
    10981 cggaaagctg gaaataggcc acttcatcag caggtgatgg cgtggcagta aaatcctcag
    11041 ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc agttgcacca
    11101 cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg ctaaacagcg
    11161 catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag gcgttcagtt
    11221 cactacgctg atggctgaac agcgccagca ccggcgcaac gcccagtttc agcagcgcga
    11281 aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt tcaccaggtt
    11341 taatgccctg acggcgtaaa ctacacgcga ggttatccgc cgcctgattc agctcccggt
    11401 aactcaactg tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg tggcgagtca
    11461 gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga cgggcaaact
    11521 cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt
    11581 tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt gacgacgcag
    11641 gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc gcgcagcgga
    11701 tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg ccgccaaaca
    11761 gttcgcggtc gaatggctcc agttcagcaa taacctgggt cgcggcctga tgcgggaaac
    11821 cgctcagcgc gggggttgga tgcagcagac aggccagagt cagtgcgttt tcttgcgaat
    11881 tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta atcaattgtg
    11941 gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc atcgcctgag
    12001 tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta cccgcttcgc
    12061 gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg gagctaaaac
    12121 gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg ccaccatcag
    12181 ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt tccagcaata
    12241 cgccactatc aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac accactttgt
    12301 cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca aacgtggttt
    12361 gttcaggaat tgcctggcgt tccaccacgt tcagcgactg gctgcgggta aaacggcggg
    12421 ctgaggtctg tttttcctgg cgggagaacg actgccagga ttcgggaata tacagcgacg
    12481 aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg atgccctgcg
    12541 ctttggcatc ggcaaacagt gcggcgagtt tttgctggaa gggactgtcg ggcgaatccc
    12601 cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg
    12661 gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag
    12721 ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa tgattatcat
    12781 ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg
    12841 caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga agcattattg
    12901 atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc tgccccctct cccttccagg
    12961 gtgagggctg gggtgagggt taatgttcgc accagtgctg gctgttcccc tcaccctaac
    13021 cctctcccca aaggggcgag gggacggatt gtgcgctttg tcgaatttgt cattacgccc
    13081 ttaaccttat taataacagg aagctgattt gtgagactcg ccccgctcta ccgcaacgcc
    13141 cttctattaa caggactttt gctttcagga atagccgcag ttcaggccgc tgactggccg
    13201 cgtcagatta ctgacagccg tggcacacat acactggaaa gccagccgca gcgtattgtt
    13261 tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt gatcgccagc
    13321 ggcgcgacca cgccgaataa ccgcgtcgcg gatgaccagg gctttttacg ccagtggagc
    13381 aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag cgccgaagcc
    13441 gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga ttcggcgctg
    13501 gcactgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta cgacgacaaa
    13561 agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa acaagcggca
    13621 gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat caaattaccg
    13681 ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa tctctggacg
    13741 ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc gaagttgccc
    13801 gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca gcttggtggg
    13861 gaaaatctgg ctgctgggtt aaatggcgag tcactattcc ttttcgccgg tgatcagaaa
    13921 gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcccgcagt acaaaacaag
    13981 caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc catgcaagtg
    14041 ctggaacgac ttaaggcgct gttttaagca ttaactgtca gacgctgtca cctgcggcgg
    14101 cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc cgataatcaa
    14161 caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc ccaaaccacc
    14221 cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca aaccgttaat
    14281 ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca gcaacgaact
    14341 aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc acatcggcat
    14401 caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca tcaataaccc
    14461 tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg cgccgagcgg
    14521 gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt cagccagcgc
    14581 cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca gcgcaatccc
    14641 tcctaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt tcaacggatg
    14701 ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta gcaaggtaat
    14761 aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccgccggtcg ccagcaataa
    14821 accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg tgatcgcccc
    14881 ggcctgcatt aagttttcac gccctaccag tgcaggtgtt gccgccagta gcgccgtaac
    14941 accaagtgat gcgaaaaaac catcccataa accaagtaaa tagattgcca gcaatgacgg
    15001 ctccggcagc agcgcgttca ggcacagtcc aatgaagcca atgccacagg tgccgcgcgc
    15061 cagcaaaatc acttttttgc gttcatagcg atccgccagc acaccgccaa ccatcaggcc
    15121 aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg tagaatgcgt
    15181 catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaga caattgagat
    15241 aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc tgaggttaag
    15301 cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa tacctattct
    15361 taggctgcac atgctaacat atccaaataa gatcgataac gataattaat ttcattatca
    15421 tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg cccggattgc
    15481 tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa tcactccccg
    15541 cttccgtagt gctggaggcc ctctccggca cctgccagag cgccgactgc accatcgtgc
    15601 tcgacgcgcg actgccgcgc acccttgccg gtttacttgc aggcggcgcg cttggccttg
    15661 ccggggcgtt gatgcaaacc ctcacccgaa atccacttgc cgaccccggc ttgcttggcg
    15721 tgaatgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt tactcatccg
    15781 cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg attgttgcct
    15841 ttaccggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg gcgggcgtgg
    15901 cgctggcggc ggtgctggaa gggctgacca gcggcatcgc cctgcttaat cctgatgtct
    15961 acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta cataccttaa
    16021 aagtggtgct gatcccggtg ctgatcgccg gagcaaccgc gctattactg agccgcgcgc
    16081 tgaacagttt gagcctcggc agtgacaccg cgacggcgct gggcagtcgc gtggcgcgca
    16141 cacagttaat tggtctgctg gcgattaccg tgctttgtgg tagtgcgacg gcggtagttg
    16201 gcccgattgc ctttatcggc ctgatgatgc cgcacatggc gcgctggctg gtaggtgccg
    16261 atcatcgctg gtcgctgccc gtcacgctac ttgctacccc tgccctgctg ctgtttgccg
    16321 atatcatcgg gcgggtgatt gtgcccggcg agttgcgcgt ttctgtggtc agtgcgttta
    16381 ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt gcatgattta
    16441 cgtctctcgc cgattactca tcacctgttt gctgcttatt tccgcctgtg tggttgcagg
    16501 tatctgggga ttacgcagcg gtgccgtcac gctggaaacc tcgcaggtat tcgccgcgct
    16561 gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt taccacgcgt
    16621 gctgatggcg ctgttgattg gcgcggcact gggcgtcagc ggtgcgattt ttcagtcgct
    16681 gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg cgtggagcgg
    16741 cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc tggcagcaat
    16801 ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta acggtatcga
    16861 cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg cctttaatac
    16921 ctggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt ggaatgccgg
    16981 atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca ttatattgat
    17041 gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg gcgatgatac
    17101 cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc tggttgcagt
    17161 ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg ctttagtcgc
    17221 gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc aggcggcgct
    17281 gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt ttatgccgta
    17341 tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta tcgtcttgtt
    17401 aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg cgaacagtta
    17461 accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat tcctgatggt
    17521 cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact gcgtacctta
    17581 agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca cattcaacat
    17641 tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc taccacgccg
    17701 ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca accgctgttt
    17761 acccgctggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc cacgggaata
    17821 actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca gcgagcgtgg
    17881 atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgacgaacc cacgacctgg
    17941 ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa ccgcgagaaa
    18001 ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta cgccagccat
    18061 ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga gattgtcact
    18121 gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga tccggtagcc
    18181 ggaacgccgc ttgtggtgcc gctcggacga acggcacctt caaccgcaaa tagttaaact
    18241 aagtggtctg ccattatggc atcctgtttt ctggatgcca tcgcatagcg caataacacg
    18301 ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat cgccttaccc
    18361 ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg cgtaaaattc
    18421 acatcgttga tatttgcttt tgttaactgc tcaacaagat actgacgatt acgtaattca
    18481 ccgttcagtt ccgcaacgtc agtgaccgct ttttcaattt ccagtttgcg ttcaataccg
    18541 tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc attactgtat
    18601 acgggctttt taattcccgc cgcgttggca atgtcgagtg aataattgag gcgctgaatg
    18661 tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc tttttcaaac
    18721 tgggttttga tctccagttt attacggacg ttttctatcg actctttcac caccaacgca
    18781 gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt tggcgcggta
    18841 aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact ggcattgtca
    18901 tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc cagttcatcg
    18961 attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta ctcttccagc
    19021 aagctaaccg actgaaactt cttgataaac aggttaaatg cttctgtacg atcaattttg
    19081 atatccagat ccagcactcg aagcttagtg aatgttttct ccagctcttg ccactgaaca
    19141 ggttctggag gcgtgacaac cgccgcgctg gtccattttt gcggcaggat gaaagagatc
    19201 agcaagcctg cgcaggcaaa cgcaaaaatg accgccatga ccgttttttt ggcccgccat
    19261 aaaactgcga ttagattaag taaatcaatt tcattattac tgggcgacgc cagaggatac
    19321 tcagaaaaat gagcgtcact tccctgttta atattcaatg atgacatggg gtataacctg
    19381 aaaatgagtc caatagggga aaatctccac ggcgctcact ttaccagttg gtgagaatta
    19441 tccgaagctg aagtttgtaa attcct
//
LOCUS       DAPPNJ010000079        19420 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000079 DAPPNJ010000000
VERSION     DAPPNJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.079"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            67..1044
                     /locus_tag="RPM73_002303"
     CDS             67..1044
                     /locus_tag="RPM73_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDX6520657.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLSAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1114..1596)
                     /locus_tag="RPM73_002304"
     CDS             complement(1114..1596)
                     /locus_tag="RPM73_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDX6520658.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1831..4413
                     /gene="leuS"
                     /locus_tag="RPM73_002305"
     CDS             1831..4413
                     /gene="leuS"
                     /locus_tag="RPM73_002305"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDX6520659.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4428..5009
                     /gene="lptE"
                     /locus_tag="RPM73_002306"
     CDS             4428..5009
                     /gene="lptE"
                     /locus_tag="RPM73_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDX6520660.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5009..6040
                     /gene="holA"
                     /locus_tag="RPM73_002307"
     CDS             5009..6040
                     /gene="holA"
                     /locus_tag="RPM73_002307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDX6520661.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6042..6683
                     /gene="nadD"
                     /locus_tag="RPM73_002308"
     CDS             6042..6683
                     /gene="nadD"
                     /locus_tag="RPM73_002308"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDX6520662.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6707..7318
                     /locus_tag="RPM73_002309"
     CDS             6707..7318
                     /locus_tag="RPM73_002309"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDX6520663.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7578..7895
                     /gene="rsfS"
                     /locus_tag="RPM73_002310"
                     /gene_synonym="rsfA"
     CDS             7578..7895
                     /gene="rsfS"
                     /locus_tag="RPM73_002310"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDX6520664.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7899..8366
                     /gene="rlmH"
                     /locus_tag="RPM73_002311"
     CDS             7899..8366
                     /gene="rlmH"
                     /locus_tag="RPM73_002311"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDX6520665.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8397..10298
                     /gene="mrdA"
                     /locus_tag="RPM73_002312"
     CDS             8397..10298
                     /gene="mrdA"
                     /locus_tag="RPM73_002312"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDX6520666.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10301..11413
                     /gene="mrdB"
                     /locus_tag="RPM73_002313"
                     /gene_synonym="rodA"
     CDS             10301..11413
                     /gene="mrdB"
                     /locus_tag="RPM73_002313"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDX6520667.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11424..12512
                     /gene="rlpA"
                     /locus_tag="RPM73_002314"
     CDS             11424..12512
                     /gene="rlpA"
                     /locus_tag="RPM73_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDX6520668.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12652..13863
                     /gene="dacA"
                     /locus_tag="RPM73_002315"
     CDS             12652..13863
                     /gene="dacA"
                     /locus_tag="RPM73_002315"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDX6520669.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13974..14237
                     /locus_tag="RPM73_002316"
     CDS             13974..14237
                     /locus_tag="RPM73_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDX6520670.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14338..14979
                     /gene="lipB"
                     /locus_tag="RPM73_002317"
     CDS             14338..14979
                     /gene="lipB"
                     /locus_tag="RPM73_002317"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDX6520671.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDLEYITA"
     gene            15238..16191
                     /locus_tag="RPM73_002318"
     CDS             15238..16191
                     /locus_tag="RPM73_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDX6520672.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTISEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16400..17365
                     /gene="lipA"
                     /locus_tag="RPM73_002319"
     CDS             16400..17365
                     /gene="lipA"
                     /locus_tag="RPM73_002319"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDX6520673.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17466..17669)
                     /gene="tatE"
                     /locus_tag="RPM73_002320"
     CDS             complement(17466..17669)
                     /gene="tatE"
                     /locus_tag="RPM73_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDX6520674.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17798..18586)
                     /locus_tag="RPM73_002321"
     CDS             complement(17798..18586)
                     /locus_tag="RPM73_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDX6520675.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18679..19062
                     /gene="crcB"
                     /locus_tag="RPM73_002322"
     CDS             18679..19062
                     /gene="crcB"
                     /locus_tag="RPM73_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDX6520676.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19117..19326)
                     /gene="cspE"
                     /locus_tag="RPM73_002323"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19117..19326)
                     /gene="cspE"
                     /locus_tag="RPM73_002323"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDX6520677.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         4869 a         4928 c         4926 g         4697 t
ORIGIN      
        1 ggcttacgca aaaatggaac acataaaaat ataatatgtt ttatctgcaa ccataaggaa
       61 atgataatga ttttcacgtc aagttgctgc gataatttat caatagatga gattatcgaa
      121 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
      181 agcgcaattg attctccgga cgacgaaaaa gccttttact ggctgaagct gtccgctgag
      241 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
      301 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
      361 ttcgcttcca acgcccttgg ctggacactg gatcgtggag aagcccccaa ttataaagaa
      421 gcggttgtct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
      481 gggtggatgt acagaaatgg caacggagtc gcaaaagact atgcgctggc atttttctgg
      541 tacaaacaag ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat
      601 gaagacggaa aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
      661 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
      721 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
      781 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
      841 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
      901 tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
      961 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
     1021 cgtgagactg aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc
     1081 atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
     1141 ctggtcatga ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
     1201 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
     1261 cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat
     1321 atctgccagc tcctgccata agctttcttt aatcacccgc ataaagacgc tgtcagattc
     1381 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc ttacagctcg
     1441 cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
     1501 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc
     1561 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
     1621 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
     1681 aattaatgta tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
     1741 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
     1801 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     1861 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     1921 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     1981 ggccacgtac gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc
     2041 aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     2101 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     2161 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     2221 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     2281 aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     2341 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     2401 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     2461 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     2521 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     2581 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     2641 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     2701 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagcggtt
     2761 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     2821 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     2881 aaatacggcc tgaacatcaa accggttatc ctggcagctg acggctctga gccagatctt
     2941 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt
     3001 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     3061 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg
     3121 ccgattccga tggtgacgct ggaagacggt accgtaatgc cgaccccgga cgaccagctg
     3181 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     3241 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     3301 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
     3361 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     3421 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     3481 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     3541 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     3601 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     3661 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     3721 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     3781 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     3841 aaacgtgtct ggaaactggt ttacgagcac acagcaaaag gtgatgttgc ggcactgaac
     3901 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct
     3961 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     4021 gagctgatga acaaactggc gaaagcacca accgatggcg agcaggatcg cgctctgatg
     4081 caggaagcac tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc
     4141 acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     4201 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     4261 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     4321 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
     4381 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     4441 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     4501 ataccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     4561 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     4621 aagaaaccac gcgtaaggac gttccatcct tgcgtttggg taaagtgagc atcgcgaaag
     4681 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtta
     4741 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagtgccaaa gtcttccgtt
     4801 cgttctttga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     4861 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     4921 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     4981 cacgcgtctc caccacgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     5041 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     5101 gcaggaaagc caggacgctg ttcgtcaggt agctgcggca caaggattcg aagaacacca
     5161 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
     5221 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
     5281 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     5341 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
     5401 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
     5461 tgctgcgcgc gcaaaacagc tcaacttaga actggatgac gctgcaaatc aggtgctctg
     5521 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
     5581 ctggccagac ggcaaattga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     5641 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
     5701 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
     5761 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
     5821 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     5881 tcgcttaagc cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     5941 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     6001 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     6061 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
     6121 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     6181 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
     6241 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc
     6301 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     6361 gtcaggattc actgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     6421 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     6481 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
     6541 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     6601 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtactg acttacatta
     6661 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     6721 aattcgtcat ggtgaaacgc aagcgaatat cgatggtcta tacagcggtc atgcgcccac
     6781 ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg catacgctgc tacatggtgt
     6841 ttcctttgat ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
     6901 tctcagtgac cgccagctcc ccgtgcaaat catccctgaa ctcaacgaaa tgttttttgg
     6961 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
     7021 gtggtgcaat gactggcagc atgcaatccc cacgaacggt gaaggatttc aggcattttc
     7081 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt cagcactatc agaatatttt
     7141 agtcgtcagc catcagggtg tactgagtct gttaatcgcc cgtttaattg gcatgcctgc
     7201 cgaggctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
     7261 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact
     7321 tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt
     7381 ctccgcagcc agactttttc cgccagacac gactttatag aaattgtttt acaaaaatgg
     7441 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
     7501 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
     7561 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
     7621 atgacctcaa aggtcaggac atcatcgcct tagacgttca gggcaaatcc agcatcaccg
     7681 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
     7741 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
     7801 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
     7861 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
     7921 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
     7981 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg
     8041 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
     8101 gcattgtcac cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
     8161 tggaacgctg gaagctggat ggtcgcgacg tcagtctact gattggcggg cctgaagggt
     8221 tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc gctgtcggcg cttaccctcc
     8281 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
     8341 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga
     8401 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
     8461 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
     8521 tgcaaattgt tcgctttacc gattaccaga cccgctctaa tgaaaaccgc attaagctgg
     8581 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggcactca
     8641 accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc
     8701 tggacgcttt gcgcagcgtg gttgatctga ccgatgacga tattgctgca ttccgaaaag
     8761 agcgcgcacg ttcacaccgt ttcacctcta ttcccgtgaa aactaacctg accgaagtac
     8821 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
     8881 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa
     8941 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
     9001 cgcatgatat cggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
     9061 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
     9121 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
     9181 ttgaaacgct gctggcgggt agccgcgcag ctgtggtagt caccgatccg cgtacaggtg
     9241 gggtgctggc actggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
     9301 ccagcaaaga ttattccgcc ttgttgaacg acccgaatac accgctggtg aaccgcgcca
     9361 cacagggggt ttatcctcct gcgtctacag ttaaacccta tgtggcggtt tcggcattaa
     9421 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag
     9481 ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
     9541 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
     9601 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
     9661 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
     9721 cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc
     9781 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc
     9841 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac
     9901 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
     9961 ttgctaaccg ccctaacggt acggcgcata aatactttgc cagcgcaccg tacaaaattg
    10021 cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    10081 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    10141 atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta
    10201 cgctgatgcg tcagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    10261 cggaaaatcc tgcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    10321 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt
    10381 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    10441 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    10501 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    10561 ggcggtagat gctttcggtg ccatctctaa aggtgctcaa cgctggctgg acctcggtat
    10621 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    10681 tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat
    10741 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    10801 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    10861 agtagtgctg gtggcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    10921 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    10981 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    11041 cggcactcag tcacagcttg aatttctccc cgaacgccat accgacttta tcttcgcggt
    11101 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    11161 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    11221 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    11281 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    11341 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    11401 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    11461 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    11521 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    11581 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    11641 atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta
    11701 acctgacggc ctctggcgaa gctttcgatc cgacacagct gacggcggcc catccaacgc
    11761 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    11821 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    11881 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    11941 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg
    12001 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    12061 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    12121 cggtaaccag tagcggtttc ctcggcgcac caacgacctt agcgcctggt gtactggaag
    12181 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa
    12241 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    12301 tgcaagtcgg ggccgttagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    12361 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    12421 gccccttcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    12481 aattacagtc atttattacc accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt
    12541 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    12601 ctatagtagg gcactttttt taattccatc acggatgtcg tagttcagac catgaatacc
    12661 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    12721 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    12781 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    12841 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    12901 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    12961 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    13021 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    13081 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    13141 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    13201 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    13261 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    13321 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    13381 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    13441 ttgatttctg cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    13501 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    13561 ttcgcctctg aaccggtttg gtttggtgat tctgatcgcg cttcgttagg ggttgataaa
    13621 gacgtgtacc tgaccattcc acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    13681 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    13741 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    13801 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    13861 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    13921 actcccgcct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    13981 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    14041 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    14101 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    14161 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    14221 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc
    14281 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    14341 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag
    14401 gctatgcatg aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc
    14461 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    14521 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    14581 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    14641 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    14701 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    14761 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    14821 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    14881 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    14941 ctactaaaca atccggactt agaatatatt accgcttaat tccatacatc aatggcccaa
    15001 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    15061 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgcaac tcaccccatt
    15121 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    15181 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    15241 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    15301 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttctga agctgtatat
    15361 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    15421 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    15481 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    15541 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    15601 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    15661 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    15721 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    15781 catgttctgt tcaccgacaa tatggtgtta atttgccgcg agggaaatcc actactctct
    15841 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaagag
    15901 caaaatttca gcggtctgcg gcagagagtt caagaaatgt ttccggaccg gcaaatcaat
    15961 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    16021 attattccca gccgttttta taacctgttt agccgctgct ggccgctgga aaaactgcct
    16081 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    16141 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    16201 gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat
    16261 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    16321 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    16381 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    16441 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    16501 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    16561 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    16621 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    16681 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    16741 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    16801 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg attgcattac
    16861 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgtgg
    16921 tcgtatggat cgtgctctgg atattctgac tgcaacgcca ccagatgtgt tcaaccataa
    16981 cctggaaaac gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt acaactggtc
    17041 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    17101 gatggtggga ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    17161 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    17221 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    17281 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    17341 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    17401 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    17461 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    17521 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    17581 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    17641 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    17701 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    17761 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    17821 agcgacggtt gtttaagacg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    17881 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    17941 ctataatccg gctttgaccg atatttttgt tcccgcactc ccccgccgcc accatataac
    18001 aggtggtatc cagcgcacga gcggcaagca acgttgacca atgatgctct ttgagcggcc
    18061 cgcgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg
    18121 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccctccacct
    18181 cgagtaacgg agcgatttca ttaccagcat caacacggcg tgattcctga atggcaaatg
    18241 catcatagag atgcaatttg gcataacggg cgacgatgtt tcctgcctgt agcgccacca
    18301 gcatattcca tgcgcgcccc ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca
    18361 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    18421 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacga
    18481 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    18541 tttcccacac agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc acctcctgtc
    18601 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    18661 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    18721 ggaagcgtgg cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    18781 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    18841 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    18901 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    18961 cgctttggct gggcgttact gaacgttttc gtcaaccttc tggggtcttt tgccatgaca
    19021 gcactggctt tctggctgtt ttcggcctca accgcacact aaagactaaa aaaaaacccg
    19081 ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc
    19141 agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt
    19201 tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc
    19261 cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt
    19321 agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg ttatcagtac
    19381 accaattgtg gtacgctttg tccagtgcaa ctttactaaa
//
LOCUS       DAPPNJ010000080        19291 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000080 DAPPNJ010000000
VERSION     DAPPNJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.080"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(60..1280)
                     /gene="fsr"
                     /locus_tag="RPM73_002324"
     CDS             complement(60..1280)
                     /gene="fsr"
                     /locus_tag="RPM73_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDX6520678.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1498..3149
                     /gene="ushA"
                     /locus_tag="RPM73_002325"
                     /pseudo
     CDS             1498..3149
                     /gene="ushA"
                     /locus_tag="RPM73_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
     gene            complement(3186..3665)
                     /gene="ybaK"
                     /locus_tag="RPM73_002326"
     CDS             complement(3186..3665)
                     /gene="ybaK"
                     /locus_tag="RPM73_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDX6520679.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3869..4663)
                     /locus_tag="RPM73_002327"
     CDS             complement(3869..4663)
                     /locus_tag="RPM73_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDX6520680.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQTAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4801..5142
                     /locus_tag="RPM73_002328"
     CDS             4801..5142
                     /locus_tag="RPM73_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDX6520681.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5256..7760)
                     /gene="copA"
                     /locus_tag="RPM73_002329"
     CDS             complement(5256..7760)
                     /gene="copA"
                     /locus_tag="RPM73_002329"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDX6520682.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8022..8954
                     /gene="glsA"
                     /locus_tag="RPM73_002330"
     CDS             8022..8954
                     /gene="glsA"
                     /locus_tag="RPM73_002330"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDX6520683.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8957..10249
                     /locus_tag="RPM73_002331"
     CDS             8957..10249
                     /locus_tag="RPM73_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6520684.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10374..10781
                     /gene="cueR"
                     /locus_tag="RPM73_002332"
     CDS             10374..10781
                     /gene="cueR"
                     /locus_tag="RPM73_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDX6520685.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10782..11240)
                     /locus_tag="RPM73_002333"
     CDS             complement(10782..11240)
                     /locus_tag="RPM73_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDX6520686.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWEWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11237..12154)
                     /locus_tag="RPM73_002334"
     CDS             complement(11237..12154)
                     /locus_tag="RPM73_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDX6520687.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12300..12977
                     /gene="fetA"
                     /locus_tag="RPM73_002335"
     CDS             12300..12977
                     /gene="fetA"
                     /locus_tag="RPM73_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDX6520688.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12964..13743
                     /gene="fetB"
                     /locus_tag="RPM73_002336"
     CDS             12964..13743
                     /gene="fetB"
                     /locus_tag="RPM73_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDX6520689.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13806..14660)
                     /gene="cnoX"
                     /locus_tag="RPM73_002337"
     CDS             complement(13806..14660)
                     /gene="cnoX"
                     /locus_tag="RPM73_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDX6520690.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14721..15530)
                     /gene="ybbO"
                     /locus_tag="RPM73_002338"
     CDS             complement(14721..15530)
                     /gene="ybbO"
                     /locus_tag="RPM73_002338"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDX6520691.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15520..16143)
                     /gene="tesA"
                     /locus_tag="RPM73_002339"
     CDS             complement(15520..16143)
                     /gene="tesA"
                     /locus_tag="RPM73_002339"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDX6520692.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16114..16800
                     /locus_tag="RPM73_002340"
     CDS             16114..16800
                     /locus_tag="RPM73_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6520693.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16797..19211
                     /locus_tag="RPM73_002341"
     CDS             16797..19211
                     /locus_tag="RPM73_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6520694.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLKVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         4486 a         5025 c         5245 g         4535 t
ORIGIN      
        1 aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct gcaatgaaat
       61 cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag tggcaggaaa
      121 gcacagattt tatagactaa ctcgatactg gtgtgatcgg cgataagccc cagaactgcc
      181 gctcccagac ctcccatacc aaaagcaaaa ccgaaaaaga gtccagaaac cataccgata
      241 cgtcccggaa gcagctcctg agcgtaaacc agaatggcag agaatgccga agcgaggata
      301 aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc
      361 gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgtcc aattttatcc
      421 cctacaggcc cgccgatcac cgtacctgcc gcaacggcaa acaggaaggc aaacagatga
      481 agctgggcat tctggataga taatccgaat ttttgcatca gataaaaggt gtaatagctg
      541 ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat gctgactgcc
      601 agtaccactt tgttgcgcgg cagtggattg attatcgtcg ctttggattt tcctttattc
      661 attcggtgct gtgccgagta ccaacggcta atttgcgcca acaccacgat cgccagcagt
      721 gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat caccgccgcc
      781 agcaagggtc ccagggaact gccaaagttg ccgccgacct gaaagataga ttgcgccagg
      841 ccatgccgcc cgccggaagc catacgggct acgcgagaag attccggatg aaagaccgat
      901 gaaccggtac cgaccagcgc cgccgccagc agaactgcgc caaaactgcc cgccagcgca
      961 agcagcacca gaccacttaa ggtaaagcac atgccaattg gcaacgacca cggcatcgga
     1021 tatttatcgg tccagtagcc gaccactggt tgcagtagcg aagaggcgag ctggaaggtg
     1081 agggttatca tgccaatctg cataaatgtc agagaaaatt ctgactgaag cagcggataa
     1141 atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct gatagcacct
     1201 aaaataccaa acgatgttcg ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg
     1261 ggttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat
     1321 cttatttgtg attattaccc gactaacata cctgtatgcg tcgcctgaag gaagtctcaa
     1381 cgccgaatac agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtc
     1441 tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga gagaagtatg
     1501 aaattattgc agcggggcgt ggcgttagcg ctgttaacca catttacact ggcgagtgaa
     1561 actgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca taccaatgat
     1621 catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg
     1681 ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc
     1741 ggtggcgaca ttaacactgg cgtgcccgag tctgacttac aggatgccga acctgatttt
     1801 cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataat
     1861 ccgctcaccg tattacgcca gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat
     1921 atctaccaga aaagtactgg cgagcgcctg tttaaaccgt gggcgctgtt taagcgtcag
     1981 gatctgaaaa ttgccgtcat tgggctgaca accgatgaca cagcaaaaat tggtaacccg
     2041 gaatacttca ctgatgtcga atttcgtaag cccgccgatg aagcgaagct ggtgattcag
     2101 gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac
     2161 gataatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct
     2221 gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg catggcggca
     2281 gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaagccaga tcaacaaaac
     2341 ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgattttgag
     2401 tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag
     2461 aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa
     2521 accagcaaat gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg cagctggaag
     2581 tgaaaatagg cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac
     2641 agactaatat ggggcggttg attctggcag cccaaatgga tcgcactggt gccgactttg
     2701 cggtgatgag cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa
     2761 acgtgctgaa agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag
     2821 aggtgattga ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc
     2881 aatttgccaa cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag
     2941 gcgaaccggt cgatccggcg aaaacttacc gtatggcgac attaaacttc aatgccaccg
     3001 gcggtgatgg atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg
     3061 atgccgaagt gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg
     3121 aaccgaaagg tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg catccggcac
     3181 aatgcttaat cgcggcgggc gatatcagca aatttggcat cgaggatctt tgccagatcg
     3241 cctgccgcca gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat
     3301 tcttgtgcag gggcgtcgat aattgttggc agacgttttt tctgccccag tgggctaatc
     3361 ccccctacca gataccccgt cgaacgctgc gcggccatcg gatcggccat ctcaactttc
     3421 ttggcaccca gcgcttttgc tactttttta agatccagtt gacctgcgac cggcgtaacg
     3481 gccacggcaa ggtgtttcat atcaccgttc actgccacca gcagcgtttt gtagacctga
     3541 tccggattca aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg
     3601 tgctcgtagg tatggatttg aaacgaaatc ttgttttttt cgagtaattt aactgcgggt
     3661 gtcatggtaa ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt
     3721 ccaaaaaaaa gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat
     3781 cgagttacac cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga
     3841 cgggccaata gcgatattgg ccattttttt agcgcaacat ctgcggcaaa ttcccttctc
     3901 catacagatg tagtgcaccg accgcgacta catagcgccc tggcggcatg gcgcgtaatt
     3961 tatcccgcca tgcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg
     4021 tattgggcag cgttatttca ttattttgcg gcggtgcatt cagccaccag ctcatcattt
     4081 gttgcagcaa ccgtgcattg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc
     4141 ctttgtcagg gagctggagc aacatggcaa tctggttttc agccccttcc agttcaatca
     4201 cgggtttatg ttgttgcttc gccgtctgca ataactggta atcgataccg tattctgccc
     4261 gcagccccag cttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt
     4321 gggtagaaaa gagcgacgga gaaatgccca tctcctggct aatgtgctgc aggttttgga
     4381 gttgctcctc gctaatacgc tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg
     4441 tatcgctggt ggaaacatca gcctcgacga tcagcgcatc ggcgtttttg agctttttga
     4501 gcaaacgagt gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat
     4561 gaaaatggcg attgccggga agggtgatat cgatggcagg ccaggtgtaa tgattaccgc
     4621 gcagcgcggc ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga
     4681 aatatcatgc tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt
     4741 gtttgtatga ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc
     4801 atgaaacagg caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg
     4861 gaaccgctcg atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatagc
     4921 gtcagtgcac tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg
     4981 aaagtttttg ataccacagt cgattttagg ctaaacctcc aggcggcagt tgatctttgg
     5041 gaagttgaaa acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa
     5101 tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca
     5161 tctcgtaggc cggataaggc gtttacgccg catccggcag tcatgcgtcg atgccaaatg
     5221 cgccgcccta aagcagcgca tccgcaatga cgtacttatt ccttcggttt aaaccgcagc
     5281 aaccggttgg cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaact
     5341 accgggttaa gcagtgttcc agtaaacggc cacaaaatac cggcggcgac cggaataccg
     5401 atgctgttgt agataaaggc accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg
     5461 gaaatcgcga gcgcatccgc aacgcccatc aggctatggc gcatcagggt aatcgccgcg
     5521 gtttcaatgg caacatcact gccgccaccc atcgcaatgc cgacatccgc ctgggccagc
     5581 gctggcgcgt cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcaga
     5641 cgtttgatcg cttcggcttt accgtccggc agcactccgg caatcacttc atcaattcca
     5701 gcttctttgg cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga
     5761 tatcccgctt tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt
     5821 acagccagca gggctactgc tttcccgtca atcgccagca gcacaggcgt tgctccttgc
     5881 gatgcctgag cagtaatctc cgcttcgata gctttggtac caacctgttg ctcatttaac
     5941 agcgcctgat tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg
     6001 cgcaatgtgc ggaaaccgtt gacctgcggc agctgcatat cacctgcttt atcgaggatc
     6061 gcccgtgcca gcggatggct ggaaccttgc tccagtgccg ccgccagacg caatgcctgc
     6121 gcttcatcaa catcagcaaa cgttttcact gcgacaacct gcggcttccc ttcggtcagc
     6181 gtcccggttt tatcgaacac gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg
     6241 tcccgcacca gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc
     6301 gtcgccagcc ccagcgcaca cggacaggcg ataatcagta ccgtggtggc aatcaccagg
     6361 gtataaacaa tttgcggtgc cggaccaaag aagtaccaga ttgccgcact gaccagcgca
     6421 atcaccacca ccacaggtac aaatacggct gagattttat ccgccagctg accgatttct
     6481 ggcttgctgc tctgggcctg gcgcaccatg cgaatgatcc gtgacagcgt ggtatggctg
     6541 ccaaccgcac tggcacgaaa cagcacgcta ccgtcctgta ccactgtccc ggcatggacg
     6601 ctttcgcctt cgcctttttg ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc
     6661 catgcttcgc cctgggtaat ctcgccatct accggcacgc gatcgccggt cgtcaggcgc
     6721 agcaacatac ctggctgcac ttccgccaga ggcacgcttt tttcaccttc gtcagtaacc
     6781 aggcgcgccg tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga
     6841 cgtgcgcgtg cttccagcat atggccgaga ttgatcagac cgataatcat cgcgctggct
     6901 tcgtaataaa gatgtcgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc
     6961 gaatagagcc acgccacgcc agtacccagc gccaccagcg tatccatcgt cgctgcaccg
     7021 ttcagcaggc ttttccatgc actgcggtaa aaatggccgc cggcgaaaac catcactgcc
     7081 agggttatca ggccgataac taaccacaag ctgcggttgt cagcggtgac catcatgtta
     7141 tcgccgatca tcccccagac cattaccggg atacccacag ccagtgcaac aattgcctgc
     7201 cagcggaagc gcttcatcgt agctacggcg gtttcttgct ggcgctcgcg gcgtttagcg
     7261 tcatcttcaa tcgcttccgc accgtagccc gctttttcca ccgcctgcac taaatcttgt
     7321 ggggaggcac tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg
     7381 acgcccggta cgctttgcag cgcattttgt acgcgggtga cacagctggc gcagctcatg
     7441 ccgctcagca gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca
     7501 gaaaccgctg tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg
     7561 tggcttacag atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg
     7621 gcagtcccgg taacgtgcgc ttcagtgata gacacatccg cttgttccac atccgggcgc
     7681 tgttcgagac tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc
     7741 aggtcgataa tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa
     7801 ggttaaacct tccagcaagg ggaaggtcaa gaaattaata aaccaggcga gtaaaagtcc
     7861 gtaaagatta aaaaattggc tcaatttgca tcaggattag acatttatct ctttgttttc
     7921 ctgaagttaa gttgcgggtg ctaagttaaa tcaagatgcc tgaaaatcgg caccggggtg
     7981 aggaattacc tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat
     8041 tacagcaggc agtggatcag gcttacaccc aatttcactc acttaacggc ggacaaaatg
     8101 ccgactacat tccctttctg gcgaatgtac ctagtcaact ggcagcagtg gctattgtga
     8161 cctgcgatgg caacatctat cgcgcgggtg atagtgatta ccgctttgcg ctggaatcca
     8221 tctcgaaagt ctgtacgtta gcccttgcgt tagaggatgt cggcccgcag gcggtacagg
     8281 acaaaattgg cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc
     8341 atggcggcaa accgctttcg ccactggtaa atgctggcgc cattgccacc accagcctga
     8401 ttaacgctga aaatgttgaa caacgctggc agcgaatttt acatatccaa cagcaactgg
     8461 ctggtgagca ggtagcgctc tctgacgaag tcaaccagtc ggaacaaaca accaacttcc
     8521 ataaccgggc cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg
     8581 aagcctgtga cgtgtacacc cgtcagtgct ccacgctcct caatactgtt gaactggcaa
     8641 cacttggcgc gacgctggcg gcggggggtg tgaatccgct gacgcatgaa cgcgttcttc
     8701 aggccgacaa cgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct
     8761 ccggtgactg ggcgtatcgc gttggtttac cgggcaaaag cggtgtaggt ggcggtattc
     8821 tggcggtcgt ccctggcgtg atgggaattg ccgcgttctc accaccgctg gacgaagaag
     8881 gcaacagtgt tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg
     8941 tgtttaaggg ctgatcatga tgaacacgga aggtaataac ggtaacaaac ctctcggtct
     9001 atggaacgtc gtttccatcg gcattggggc aatggtgggg gcggggatct tcgcgctgct
     9061 ggggcaggct gcattgctaa tggaagcctc gacctgggtc gcctttgctt ttggcggtat
     9121 tgtggcgatg ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg
     9181 cggcattatc gacttctttc gtcgcggatt aggcaacggc gtcttttcgc tagcgctctc
     9241 gttactgtac ctgttgacgc tggcggtgag catcgccatg gtcgcccgtg cttttggcgc
     9301 ttatgccgtg cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc
     9361 gttggggatc attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg
     9421 gctggaagtg atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg
     9481 tgtctggtcg ttgcaaccgg cgcatatttc tgtctctgcg ccccccagct ccggtgcgtt
     9541 cttctcctgt atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc
     9601 ggcggataaa gtgaaagatc cgcaggtcat tatgccacgg gcgtttctgg tggcaattgg
     9661 cgttaccacg ttgctttata tctcgctggc gctggttttg cttagcgatg tatcggcatt
     9721 agagttagaa aaatatgccg ataccgccgt agcgcaggca gcttctccgc tgctcgggca
     9781 tgtgggttat gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccattaacgc
     9841 gaacctgttc gccgtgttta acatcatgga caacatgggc agcgaacgcg aactaccgaa
     9901 gctaatgaat aaatccctgt ggcggcagag tacctggggc aacatcattg tcgtggtgtt
     9961 gattatgctg atgacggcgg cactgaattt aggctcactc gccagcgttg ccagcgccac
    10021 ctttttgatt tgctacctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca
    10081 cgcctcgttg ccgattctta tcgttggtac gttggtgatg ttgttggtga tcgttggctt
    10141 tatctacagt ctgtggtccc agggtagccg tgcgttgata tggattattg gctcactctt
    10201 actcagcctt attgtggcaa tggtcatgaa gcgcaataaa accgtataac atctctctgt
    10261 gcgcagtact tcctgtatta ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg
    10321 cttgaatatc tgttgaaacc ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca
    10381 tcagcgatgt agcaaaaatt accggcctga ccagcaaagc cattcgcttc tatgaagaga
    10441 aggggctggt gacgccgccg atgcgcagcg aaaacggtta tcgcacctac acgcagcagc
    10501 atctcaacga actgacctta ctgcgccagg cacggcaggt gggctttaac ctggaagaga
    10561 gcggcgagct ggtgaatctg tttaacgacc cgcagcggca cagcgccgac gtcaaacggc
    10621 gcacgctgga gaaggtggcg gagatcgaac gacacattga ggagctgcaa tccatgcgcg
    10681 accagctgct ggcactggcg aatgcctgcc ctggcgatga cagcgccgac tgcccgatta
    10741 tcgaaaatct ctccggctgc tgtcatcatc gggcagggtg attaagacga gacggctcgg
    10801 atatgtaggg ttatcccttc tatcgcaatg acttcaacat gcgtacctgc accgagatcc
    10861 tcgctggcgc tgacaggcca tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc
    10921 agcggagatt ccagcacaaa acgtcggcca atcagctgct gcccgcgctg gtttaaatga
    10981 ctatcgctgt gcttttgttc gcgcacccgc cgcgacaacc attcccacca cagccaggcg
    11041 gcgagcagcg tcaggatggc aaacatcacc ccttgccact cccaacccag cggcaccagc
    11101 cagaccacca ggccggtaat caccgctgac acgccgctcc acagcaaata accatttccg
    11161 cccagcatct cggctgccag cagcaacccg ccgagactga gccagaaaat atgtggatga
    11221 acgaccatta actccatcat ggctgagtcc gtttgttggc gctgtctttc accagctcgg
    11281 caatcccggc aatcgacccc atcaggctgc tggcctctaa tggcatcatc actactttgc
    11341 tgttactgga ggaaccgatc tgctgtaacg cttcggtgta tttctgcgct acgaagtagt
    11401 tcaccgcctg aatatcaccg gaggcaatgg cttcagatac cattttggtg gcgcgggctt
    11461 ctgcttcggc agaacgttca cgcgcttcgg cctgtaagaa tgccgactga cgttcacctt
    11521 ccgctttcag aatttgcgac tgtttttcac cttccgcttt gagtatttcc gcctgacgga
    11581 tcccttccgc ttcaaggatg taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca
    11641 ttgaagagat aagctctgcc ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa
    11701 tcccccacgg gttggtggct tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc
    11761 gctgagagag catttcgtca agttccattg aacccaacac ggtacggatg ttagtcatgg
    11821 tcaggttgat gatcgccagc tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa
    11881 tcacctgaat aaaacagact gcgtcgatgg taacgttggc gttatctttc gagataactt
    11941 cctgggaagg gatatcgagc acttgctcca tcatattgat cttgcgacca atgcgatcca
    12001 taaacggcac caccagactg agccccggct gtaacgtttt ggtatagcga ccaaaacgtt
    12061 ctaccgtcca ttgatagccc tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg
    12121 cgacaaaaat gagaatcggg ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc
    12181 ataatcagca aaattgctgc ttgattaaac aaattatacc tgattactga aagagagttc
    12241 ccccttattc ctgcgaagga taaactgttt ttagtaaaaa tcagaaaaag ggaacagtga
    12301 tgcaggaaaa tagtcctttg cttcagctac aaaacgtagg atatctggcg ggtgatgcga
    12361 agattcttaa taacatcaat ttttcgctgc gtgctggcga atttaagtta attaccggtc
    12421 cttccggttg tggcaaaagt acgctgctaa aaatagttgc ttcattgatc agcccaacca
    12481 gcggaacgtt actgtttgaa ggtgaggatg tcagcacact aaagccagaa atctaccgcc
    12541 agcaagtctc ttactgcgcc cagacgccga cgctgtttgg cgatacggta tacgataatc
    12601 tgatctttcc ctggcagatc cgtaaccagc agcctgaccc ggccattttt ctcgattttc
    12661 tcgaacgctt cgctttgccg gacagcattt tgacgaaaaa tatcgccgag ctatctggtg
    12721 gtgaaaaaca acgcatctca ctgattcgta acctgcaatt tatgccgaag gttttattgc
    12781 tggatgaaat caccagtgcg ctggatgaaa gtaataaaca taacgttaat gagatgatcc
    12841 atcgttatgt gcgcgagcaa aatattgccg tgctgtgggt gacacacgat aaagacgaaa
    12901 ttaatcatgc ggataaagtg attacactgc aaccgcatgc cggagaaatg caggaagcac
    12961 gctatgaact cgcataatat tactaacgaa tcattggcac tggcattaat gctggtggtg
    13021 gtggcaatct taattagcca taaagaaaaa ctggcgctgg agaaagatat tttctggagc
    13081 gtcgggcgag cgataattca gctgattatt gtcggctatg tgctgaagta tattttcagc
    13141 gtggatgatg ccagcctgac attattgatg gtgttattta tctgctttaa tgcggcgtgg
    13201 aacgcgcaaa aacgcagtaa atatattgct aaagctttta tctcatcgtt tattgccatt
    13261 acggtcgggg cgggaattac cctggcggtg ctgattctct ctgggtcgat tgaatttatc
    13321 ccgatgcagg tgatccctat cgccgggatg attgccggta acgccatggt agcggtgggg
    13381 ttgtgttaca acaatttagg gcaacgggtc attagcgaac agcaacagat tcaggagaaa
    13441 ctgagtcttg gtgcgacgcc gaagcaggct tcagcgatat tgattcgcga cagtattcgc
    13501 gcggctttaa ttccgacggt cgattcagca aaaacggttg gcttagtgag tttaccagga
    13561 atgatgtccg ggctgatatt tgccgggatt gatccggtga aggcgattaa atatcagatt
    13621 atggtgacct ttatgctgct ctcaaccgcc agcttgtcga ccattattgc ctgctattta
    13681 acctatcgta agttttataa ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa
    13741 tgatgcgatg ccggatgcgc agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg
    13801 gaatatcaat acaacaatgc atacagctgg cggcgatact tcgacgccag tgcatcaccc
    13861 gtacccagcg cagcgaggat ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg
    13921 agatctttac gcagatgccc gaacagcaac tccagcgcct cttcattgcg cccaacctgg
    13981 tgcagttgca gagccagttg cgtcgccagt gcggcatctt ctggattctc cgccacctgc
    14041 tgttgcaact gttgaatttc cggcgtatca gccgcctgct tcagcagttc gatttgcgcc
    14101 accagcccct ggtagcgggt gtcctgatcc tgcaacggaa tggttttcag caccgcttcc
    14161 gcatcttcag aacggttcag cgcaatcagc gtttctgcca gcagcaggcc gatctccccg
    14221 ttctgattcg acaactgcca ggcgtctttc agcaatggca gggcatcggt gtaattgcct
    14281 tcctgcatca gttgcatcgc ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta
    14341 tccagcaggg cgcggatcgc ctcttccggt tgcggcccct ggaagccatc taccggttgc
    14401 ccgttctgga acagatacac ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc
    14461 tgctccgcgt cacagtccag cttcgccaga ataaattgcc cgttgtactg cgccgcgagg
    14521 ctttccagga tcggggttaa ctgcaaacag tgctggctac gttcagacca aaaatagaac
    14581 agcaccggag tggtcatcga ctgttcaaga acctgttgca ggttagattc gttaatgttg
    14641 acaatatttt ctacggacat ggagtcgctc tctgttgtcg atattttctt tgacatgggg
    14701 gcttaagcgc gcgcttcaac tcacccctgc aatattttgt ccatcacgcg ccccggcagc
    14761 aggcgcttga gcaccattac tgcccaggtc accagcgtta ccggatagcg catcttcggc
    14821 ttctcgctaa taaaagcatg gcgtactttg tccaccaccg cttccggtcc caacgtaaag
    14881 cgggcggcga tgccgggatt ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg
    14941 gtgaagcgag tacgaatggg accgggttcg atcaggctga ctttaattcc gctgtggcgc
    15001 agctccatgc gcagtgcgtc tgaccacgcc tccagcgcat atttactggc cgcgtaagcg
    15061 ccacgacccg gcgtggagat taatcccatc accgatgatg tcatcacaat acgcccttca
    15121 ccgtgcggta acatcgcggg taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg
    15181 gcggaaaact gctgttccat ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg
    15241 aatccggcat tgttaaagat cccatacaga caattatcgg tcagggcgat cacctcgtct
    15301 gctgcgcgat caacactttc tggtgaatcc agatcgatca acacgccggt aaatcccatg
    15361 ttgttcatac gctcaacatc atccggtttt cggcaacctg ccagcacatg aaaaccctgg
    15421 cgttttaatt cgagcgcgct ttccaggcca attccactgg aacatccggt aattaagacc
    15481 gatttttgca taactttacc tgtcaggatc tccgttgctt tatgagtcat gatttactaa
    15541 aggctgcaac tgcttcgcca tccagtcggc aataaacggc tgggcgtcgc ggttgggatg
    15601 aataccgtca tcctgcatcc attgtggctt gaggtagacc tcttccataa aaaagggcag
    15661 cagcggaaca tcaaactctt tggcgagttt ggggtaaatg gcgctaaagg cttcattata
    15721 acggcgacca tagtttgcag gcagacgtat ttgcattaac aatggttcag cgttggcggc
    15781 tttgacatcc tgcaaaatct ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg
    15841 caaaccgtca ttgccgccca gttcaaccag cacccaacgc ggctgatgct gtttcagcag
    15901 agccggaagg cgtgccagtc cttgttgcga ggtgtcgccg ctgatgctgg cattaactac
    15961 cgacgtttta ctctgccact tatcattcaa caaggcaggc caggccgcgc tggcagacat
    16021 tcgatacccg gcgctcaggc tatcacccag aatcaataac gtgtccgctg cggcggcacg
    16081 gaaggttaac aggaccagga acaggaaggg caaatgccag cggaaaacat tgttgaagtt
    16141 catcatctta agaagtccgt cggtcagggg gagcatgaac tctccatcct caccggagtt
    16201 gagctggttg tcaaacgtgg cgagaccatc gcactggtgg gcgagtcggg atcgggtaag
    16261 tcaaccttgc tggcgatcct cgccgggctt gatgacggca gcagtggcga agtgagtctg
    16321 gtgggacaac cgctacataa tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac
    16381 gtcggctttg tttttcagtc atttatgtta attcctaccc ttaacgcgct ggaaaacgtc
    16441 gagcttccgg ctctgctgcg cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg
    16501 ttgctcgaac agttagggct gggtaaacgt ctggatcatc ttccggcaca gctttccggc
    16561 ggtgaacagc aacgagtggc gctggcacga gcctttaatg gtcgacctga tgtgctgttt
    16621 gccgacgaac ccaccggcaa ccttgaccgc cagacgggcg ataaaattgc cgacctgctg
    16681 ttttccctca accgtgaaca tggcaccacg ttgattatgg tgacccacga cctgcaactg
    16741 gcggcacgct gcgaccgctg cttacggctg gtgaacgggc agttgcagga ggaagcatga
    16801 ttgcacgttg gttctggcgc gaatggcgtt cgccgtcgct attaattgtc tggctggcgc
    16861 taagcctggc ggtggcctgc gtgctggcgc tgggcaatat cagcgatcgc atggagaagg
    16921 gcttaagcca acaaagccgg gagtttatgg cgggcgatcg ggcgttgcgc agttcgcgcg
    16981 aagtgccgca agcatggctg gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc
    17041 tgactttcgc caccatgaca tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg
    17101 tggatgatat ctacccgatg tatggcgatc tgcaaactaa tccccctggc ctgaaaccgc
    17161 aggcgggcag cgtattgctg gccccacgcc tgatggcact gcttaacctg aaaacgggcg
    17221 acaccattga cgtgggcgat gccaccttgc ggattgccgg agaagtgatt caggaaccgg
    17281 attccggttt taaccccttc cagatggctc cgcgtctgat gatgaatctg gcggatgtcg
    17341 ataaaaccgg agccgtgcag ccggggagtc gggtcacctg gcgctataaa ttcggcggca
    17401 gtgagaacca gctcgacagc tatgagaaat ggttgttacc tcagcttaaa cccgaacaac
    17461 gctggtacgg tctggaacag gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac
    17521 agttcctgct gctttcggcg cttctgacct tgctgctggc agtggcagca gtggcggtag
    17581 cgatgaatca ttactgtcgc agtcgctacg atctggtggc gatcctcaaa acgctggggg
    17641 cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg ctgacgcttt
    17701 cagccgttac cggtggggcg ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca
    17761 agcccgttct gcctgctgca ctaccgccag ccagcctctg gccgtggcta tgggcgctgg
    17821 gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg ttgctggcaa
    17881 cgcagccttt acgcgtatta cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt
    17941 atctgccgat tgtcagtgta gtggttgtgc tgctgctcgc cggattaatg gggggcagta
    18001 tgctgctctg ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg
    18061 gctggatgct gctgaatgta cttcgccgca tgacgctgaa atcgctgcct ctgcgcctgg
    18121 cggtcagccg cctgttacgt cagccgtggt caacgttaag ccagctttcg gcattttcgc
    18181 tctcctttat gctgctggca ctgctgctgg tgttgcgtgg tgatctgctc gaccgctggc
    18241 agcagcagct acctccagaa agcccgaact actttttaat taacatcgcc acagaacagg
    18301 tagcaccgct aaaagcgttc ctcgcggaac atcagatagt cccggaatcg ttttatccgg
    18361 tggtgcgggc gcggctgacg gcgattaacg ataagccgac agaaggtaat gaagatgagg
    18421 cgcttaaccg cgaactcaat cttacctggc aaaatacgcg gcccgatcat aatccgattg
    18481 tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac
    18541 gcttaaaggt tgccctcggt gataccgtga cttttatggg cgatacccag gagttccgcg
    18601 ctaaagtgac cagcctgcgc aaagtggact gggaaagtct gcggcctaat ttctatttta
    18661 ttttcccgga aggggcatta gacgggcaac cgcagagctg gcttaccagt ttccgctggg
    18721 agaatggcaa cggcatgttg acgcaactca accgccagtt cccgaccatt agcctgttag
    18781 acattggcgc gattttaaaa caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg
    18841 aggtgatggt ggtgctggtc accgcctgcg gtatgttgct gttgctggca caggtgcagg
    18901 tgggtatgcg tcagcgccac caggagctgg tggtgtggcg cacactcggt gcggggaaaa
    18961 aactgctgcg taccacgttg tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg
    19021 tggccgcaat tggtgcggaa acggcgctgg cagtgttgca ggcgaaagtg tttgattttc
    19081 cgtgggagcc agactggcga ttgtggattg ttctgccgtg cagcggagcg ctgctgctgt
    19141 cgcttttcgg cggctggctg ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt
    19201 ttgcggggtg atgagagtaa ataaggggcg ttctggttga atggaacgcc ttgtttaaat
    19261 taattctaca atatgtttca ttgatgatgt t
//
LOCUS       DAPPNJ010000081        19107 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000081 DAPPNJ010000000
VERSION     DAPPNJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.081"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(155..802)
                     /gene="ychE"
                     /locus_tag="RPM73_002342"
     CDS             complement(155..802)
                     /gene="ychE"
                     /locus_tag="RPM73_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDX6520695.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1279..3954
                     /gene="adhE"
                     /locus_tag="RPM73_002343"
                     /gene_synonym="adhC"
     CDS             1279..3954
                     /gene="adhE"
                     /locus_tag="RPM73_002343"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDX6520696.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4078..4978
                     /locus_tag="RPM73_002344"
                     /pseudo
     CDS             4078..4978
                     /locus_tag="RPM73_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5260..5877)
                     /gene="tdk"
                     /locus_tag="RPM73_002345"
     CDS             complement(5260..5877)
                     /gene="tdk"
                     /locus_tag="RPM73_002345"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDX6520697.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6483..6896
                     /gene="hns"
                     /locus_tag="RPM73_002346"
     CDS             6483..6896
                     /gene="hns"
                     /locus_tag="RPM73_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDX6520698.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7040..7948)
                     /gene="galU"
                     /locus_tag="RPM73_002347"
     CDS             complement(7040..7948)
                     /gene="galU"
                     /locus_tag="RPM73_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDX6520699.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8150..9163)
                     /gene="rssB"
                     /locus_tag="RPM73_002348"
     CDS             complement(8150..9163)
                     /gene="rssB"
                     /locus_tag="RPM73_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDX6520700.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9255..10160)
                     /gene="rssA"
                     /locus_tag="RPM73_002349"
     CDS             complement(9255..10160)
                     /gene="rssA"
                     /locus_tag="RPM73_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDX6520701.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10273..10731
                     /locus_tag="RPM73_002350"
     CDS             10273..10731
                     /locus_tag="RPM73_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDX6520702.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10781..11623
                     /gene="purU"
                     /locus_tag="RPM73_002351"
     CDS             10781..11623
                     /gene="purU"
                     /locus_tag="RPM73_002351"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDX6520703.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11783..11867
                     /locus_tag="RPM73_002352"
     tRNA            11783..11867
                     /locus_tag="RPM73_002352"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11817..11819,aa:Tyr,seq:gta)
     gene            11915..12046
                     /locus_tag="RPM73_002353"
     ncRNA           11915..12046
                     /ncRNA_class="other"
                     /locus_tag="RPM73_002353"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12230..12907)
                     /gene="narI"
                     /locus_tag="RPM73_002354"
     CDS             complement(12230..12907)
                     /gene="narI"
                     /locus_tag="RPM73_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDX6520704.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12907..13617)
                     /gene="narJ"
                     /locus_tag="RPM73_002355"
     CDS             complement(12907..13617)
                     /gene="narJ"
                     /locus_tag="RPM73_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDX6520705.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13614..15152)
                     /gene="narH"
                     /locus_tag="RPM73_002356"
     CDS             complement(13614..15152)
                     /gene="narH"
                     /locus_tag="RPM73_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDX6520706.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15149..18892)
                     /locus_tag="RPM73_002357"
     CDS             complement(15149..18892)
                     /locus_tag="RPM73_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDX6520707.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNDNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4820 a         5008 c         4576 g         4703 t
ORIGIN      
        1 tgaggagaag cagttaagga aatccttata actaactgta atttgagaag aatattttta
       61 cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg acgaagtaaa
      121 cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg gggaaaatcc
      181 ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc cccataatac
      241 gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt gccatgcgga
      301 acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca aacagatagc
      361 taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct ggccccgcca
      421 tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt tctgattttt
      481 cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc gctatcgtca
      541 ccaccaggat gcccccggcg atacggaacg aatcaattga tataccaaaa agttgcagaa
      601 tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg ttggctgtaa
      661 ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata aagacgggaa
      721 taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag taaacgggaa
      781 aatcaaaaaa ggtctgaatc acggttagct ccgaagcaaa agccggataa tgttagccat
      841 aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt cacctcctaa
      901 ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat gctaatgtag
      961 ccaccaaatc atactacaat ttattaactg ttagctataa tggcgaaaag cgatgctgaa
     1021 aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat cagtacccag aagtgagtaa
     1081 tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg tttaaactct
     1141 tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gattttcata ggttaagcaa
     1201 atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa aaaagtttaa
     1261 cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc actcgtagag
     1321 cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt agacaaaatc
     1381 ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa aatggccgtt
     1441 gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt tgcttctgaa
     1501 tatatctaca acgcctataa agatgaaaaa acctgtggtg ttctgtctga agacgacact
     1561 tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt tccgaccact
     1621 aacccgactt caactgctat cttcaaatcg ctgatcagtc tgaagacccg taacgccatt
     1681 atcttctccc cgcacccgcg tgcaaaagat gccaccaaca aagcggctga tatcgttctg
     1741 caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga tcaaccttct
     1801 gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct cgcgactggt
     1861 ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg tgtaggcgcg
     1921 ggcaacactc cagttgttat cgatgaaact gctgatatca aacgtgcagt tgcatctgta
     1981 ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc tgttgttgtt
     2041 gttgactctg tttatgacgc tgtacgtgaa cgttttgcaa cccacggcgg ctatctgttg
     2101 cagggtaaag agctgaaagc tgttcaggat gttatcctga aaaacggtgc gctgaacgcg
     2161 gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc tgtaccagaa
     2221 aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc gttcgcacat
     2281 gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga cgcggtagaa
     2341 aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct gtacactgac
     2401 caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac ggctcgtatc
     2461 ctgattaaca ccccagcgtc tcagggtggt atcggtgacc tgtataactt caaactcgca
     2521 ccttccctga ctctgggttg tggttcttgg ggtggtaact ccatctctga aaacgttggt
     2581 ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat gttgtggcac
     2641 aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct ggatgaagtg
     2701 attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt caacaatggt
     2761 tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga agtcttcttc
     2821 gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact ggcaaactcc
     2881 ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc cgcgaagatc
     2941 atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct gcgctttatg
     3001 gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa aatgatcgct
     3061 gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt aactgacgac
     3121 gctactggtc agaaataccc gctggcggac tatgcgctga ccccggatat ggcgattgtc
     3181 gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg tctggacgca
     3241 gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc tgatggtcag
     3301 gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca cgaagggtct
     3361 aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg tatcgcgttt
     3421 gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc ccagttccat
     3481 attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta caatgcgaac
     3541 gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc tcgccgtcgt
     3601 tatgctgaaa ttgccgacca cctgggtctg agcgcaccgg gcgaccgtac tgctgctaag
     3661 atcgagaaac tgctggcatg gctggaaacg ctgaaagctg aactgggtat tccgaaatct
     3721 atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa actgtctgaa
     3781 gatgcattcg atgaccagtg caccggcgct aacccgcgtt acccgctgat ctccgagctg
     3841 aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggtga aactgcagcg
     3901 aagaaagaag ctgctccggc taaagctgag aaaaaagcga aaaaatccgc ttaatcagta
     3961 gcgctgtctg gcaacataaa cggccccttc tgggcaatgc cgatcagtta aggattagtt
     4021 gaccgatcct taaactgagg cactataacg gcttccacaa cagggagccg ttttcttatg
     4081 ccacttctca atgatctgct cgatttcagt gaccatccgc ttatgcctcc gccctctgca
     4141 caactatttg cagaacacct tcccaccgag tggatacaac actgcctgac gctttctgct
     4201 catgcgaccg ttcgccgccg tcgtttaccg ggggacatgg ttatctggat ggtggtgcaa
     4261 tgagccaatt accgatgttg ttcgccgtct gaacctgagc gcggatggcg aagcggggat
     4321 gaacctgctg gcccgcagcg ctgtcaccca ggcgcgtcag cgcgtggggg ccgccccagt
     4381 ggaatggctc ttccgccaga ccgcacagac aggggcgcgg aacgttacct gaaggatgac
     4441 tggcacggcc tgcaactttt tgccattgat ggcgcacagt tcaggacacc tgactgataa
     4501 acccgagctg cgtgaatatt atgggtctgc caacacatcc actaagcggc agaacgccta
     4561 cccggttatg cgtctggtgg ccttaatgaa cctgggaagt cacattctgc tgaatgccgt
     4621 gaccgcacct taccgacaga gcgaaactgt gctggcccac tccatgctcg ccaccatccc
     4681 ggataactct attacgttgt ttgataaact tttttacagc gaagacctgc tgctgacgct
     4741 gaaccagaag ggttgtaacc gccactggct actgcctgcg tggaagaata tcgcatcaga
     4801 aatgatagag ctgggaaaca cggcgtcacc agggaccatc ccgaaacgac tggagcatct
     4861 tcggggtgct ctggaagtgg tgttcataac aaaacgcccc cgaccatcaa ggccgaggtc
     4921 ggttaagata tcaaaaaccc gttatcctgt gaaacatagt gccgctcctc ttaagtgaac
     4981 ggcattgccc ttctggggcc gtattttttg tttacccaaa gcaacttttc cataaaccga
     5041 cagcattagc cttcatcata tttgcgacga tgtataacgc ctaaacacag ggatattgta
     5101 ctttacaggt cacaagtcaa cgtcggtgct taagagccct gtgaggcgta tagcggcgtt
     5161 aaaaaactgc cgagaagggt atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat
     5221 aaaagtgccc tgctcaccct gtcagtaaag aaattcttat taatcgtggc gatgcctttc
     5281 ctgaatagcc gttaatgagc cgacttgtaa cgcctcttta tagtgtttac ggcatacaga
     5341 aacgtatcgt tcattaccac caattaccac ctgctcacct tcgttataag gtctgcctgc
     5401 ttgatcaaga cgcagcacca tgcttgcttt acggccacaa aaacagatgg tttttaattc
     5461 aaccagtttg tcggaccatg ccagtaagta ttggctgcca ataaataatt cacctcgaaa
     5521 atcggtacgt aaaccataac aaagtacggg tatatcgagt tgatcgacaa cctccgataa
     5581 ttcatatact tgttgtctgg ttaaaaactg gcattcatca accagtacgc aatgaattgc
     5641 ctgctgttca tgttccgcac gaatctcatc aaataatgat gaattttggt taaataattt
     5701 tgcaggcgat gacaaaccta tacgcgaact gactttcccg gcaccaaagc gatcatcaat
     5761 ttctgccgta tatacgacag tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa
     5821 caatgctgta gacttacccg cattcattgc ggaatagtag aaatatagct gtgccatcag
     5881 ccacaggccc tcaatgatat gaaataaagt tggctggagt ttatcataat tcgtcagttt
     5941 ttcagctcaa cagtatgcac agaagtgggt atatgcgttc tcccttacga agccttgcat
     6001 aatccttctg agctatcatt acaactgcct cgcttgttat aagcgggtaa atgactgctg
     6061 gtaactattc acaatcttta acctgttgcg caagtaatag ccctctgttg acctccagga
     6121 gatagtgcaa tactaagtcc atgctcttat tgcgactgtt ctacttttca tcattcgctt
     6181 aatagggaat tctcgtaaac acaactaata cagaagactg aaaggtcgtc agcctacgat
     6241 aatctcccca taaaatgtga catgaatcag gaagttttaa cctcacgtgc tgcgaaatca
     6301 tcggtgtaaa tagggctata tgccgcgtct tttctggcta attttatgaa aagatattta
     6361 ttggcggcac aaaataaaga acaattttga attccttaca ttcctggcta ttgcacaact
     6421 gaatttaagg ctctattatt acctcaacaa accaccccca atataagttt gagattacta
     6481 caatgagcga agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag
     6541 aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac
     6601 gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc
     6661 aatatcgcga aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg
     6721 ctgccgttaa atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg
     6781 ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca
     6841 aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt
     6901 ttgtagattg cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa
     6961 gacaatttaa taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg
     7021 gataacaccg atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa
     7081 attccgtgcc aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta
     7141 atttattacc gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt
     7201 cgatcagcat atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt
     7261 ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag
     7321 acggcgcaac atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg
     7381 gcgctaattc aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt
     7441 caaccatgat ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat
     7501 cctgtgacaa atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg
     7561 gttcatcacc cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac
     7621 cctgacgaac ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca
     7681 gttgacgttt tacacgtttt tccagcattg cttccagttc aaaactggta tcaaagtggt
     7741 tttcaataga gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa
     7801 tacattcatt cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg
     7861 ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt
     7921 ttttgacttt cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac
     7981 gtgttcatat ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga
     8041 aaaggaggcg agttagcata attaagacaa ttacttatac gaatggcgac gatagtacca
     8101 ccagccgaca ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag
     8161 acaacatcaa gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc
     8221 gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga
     8281 tttgcacctg gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga
     8341 gatttttcag ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg
     8401 cctgacgaag taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat
     8461 tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag
     8521 caagtacgcc attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt
     8581 ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacgat
     8641 aattaacccg gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt
     8701 tcgccgctgc ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt
     8761 cctcaacgcg cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc
     8821 gattcagatc tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg
     8881 caatatctgc catattttca gtggcagata tcaccagaac cggggtctgg tcgcctctgt
     8941 tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca
     9001 tcaggtctgg agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta
     9061 ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat
     9121 cttcaacaat gagaatctgt tttccgacca atggctgcgt catgttctct cccctgactg
     9181 gctttactca atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta
     9241 gataaaaatt ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt
     9301 tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat
     9361 atttgcgggc aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt
     9421 ttaaggcggt tctccagcac ctggatagaa gtggtcataa tctctgttgc cgttggcgct
     9481 gtcaccgccc gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggc
     9541 agagaatcac cattctcgct attttcttca ctgacattaa aggagagcaa atcttgttgc
     9601 atcaaatgag catcgtgctg caggtcaacc gctatcacaa tatcagcccc caatgcacgc
     9661 gtgagggaaa taggaattgg gttaacgact gctccatcaa ccagccagta gccgttatgt
     9721 gcaacaggtg ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg
     9781 ccttcagtaa accataattc acgtcccgta cttaaattgg tggcaacagc cgcaaagcga
     9841 cgggaacaat tttcgatctc tgtttccggc attatttcgc ggtattgatt gaagacacgc
     9901 tcgccgcgca gtaacccacc gcgctgccag gagagatcca tcaggcgtaa aacatcccaa
     9961 taactgaaag aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg
    10021 cccaccagcg aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt
    10081 agcgcattaa taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc
    10141 cctattttta tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca
    10201 tcgctactgc tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca
    10261 gggagattat ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt
    10321 tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg
    10381 cgttactgcg cttttgtgat gcaagacgca gattatttaa taaagacctg gcatccatct
    10441 tgtggagcag cggcgttacg tgccgaattg attgccggat ttgcgcatac tgaatggttg
    10501 ggattgaccg tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt
    10561 gttgctcgat ttaccgaagg tggtaaaaca ggtgcaataa ttgaacgttc gcgatttttg
    10621 aaagaaaacg gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat
    10681 ccctgccctt gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg
    10741 gtacggttta gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa
    10801 agttctgcgt actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg
    10861 ctacaagcac gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg
    10921 cttttttatg cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct
    10981 cgatagcgca ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat
    11041 agtgattctg gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta
    11101 cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct
    11161 ggttgagcgt tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga
    11221 gcacgatcaa aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc
    11281 gaagtatatg cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa
    11341 tattcaccat tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga
    11401 acgtggtgtg aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg
    11461 cccaatcatc atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat
    11521 gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc
    11581 acagcgcgtc tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac
    11641 tggttacctt taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta
    11701 acactttaca gcggcgcgtc atttgatatg atgcgccccg cttcccgata agggagcagg
    11761 ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa
    11821 atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca cttttcaaaa
    11881 gtccgtaaac tctcaaacga atccgcaatt aaatattctg cccaagcggg gaaggatgag
    11941 aagcttcgac caaggttcga cttgagcgcc agcgagagag cgttgccgca ggcaacgacc
    12001 cgaagggcga agcgcgcagc gctgagtaat ccttccccca ccaccatcgc ttccttaaat
    12061 aatcaaaacc tgttactccg agctaatttt gcccgatggc accacgttta ccgggcctac
    12121 aatatcctcc cctgtaggtc ggataagcga agcgcatccg acaactcacc ccgcaccgtg
    12181 cccataaaaa aaccccgccg aagcagggtc tatgtgaact aaaattcgct tagtgacgag
    12241 cgcgcaccag ctggtactta cgtgtcagat actccaccgg tacgctccag atgtgtacca
    12301 gacgcgagaa cgggaacagc aggaataacg tcatccccag caccaggtgc agacggaaga
    12361 taaacgccac accatcgagg tgttgagaag cgccaccgtg gaaggtcacc accgactgcg
    12421 cccagccaac cagtttcatc atctcgctac cgtccatatg ctgagcggag aacggaatgg
    12481 tcaacaggcc cagcgcgcac tggataacga gcagcgacag gatcaggata tccgctccgg
    12541 tagtggttgc acgcacgcgt gggctgaaca gacgacgctt cagcaacagc acgccgccaa
    12601 tcagacacag cacaccgctg gcaccaccag caaacatcgc cattttctgt ttcacttcaa
    12661 tcggcagcca ggcttcatac atccagtgcg gcgtcagcat accgaagaag tgaccaacaa
    12721 aaatccccag aataccgata tggaacaggt tcgacgccag gttcatccct ttgcgatcca
    12781 gcatctggct ggacgccgcg cgccaggtgt actgcccgta gtcataacgc agccagctac
    12841 caatcaggaa gaccgccccg gctatgtacg ggtagatatc aaagaagaac atattcagga
    12901 attgcattag tgctgtcctc cggtggtgat attcagatat tgcggcgcga cggcaccggc
    12961 aaagcgacgc tgatgagcag tgattgctga atcaccgcag cctttgtcag caaagaattt
    13021 aacctgctct tcttcccaga cagcatccag cgcctgcggc gtatcatcgc gcgcttcgtc
    13081 ggcaattttt tccgccactt tgtcgctgtc gatagcggta ttggccaatt tcagcagcag
    13141 atcaaacatc acggcataac ggctttcacg ctgttgcaga cgcgcgctca gcaatgccag
    13201 aatcggcgcg atatctttca aaccttccac ggcttcgctt tgcggcagct gcgacaggta
    13261 ctccagatac agcggcagat ggtccggtaa ttcgcggctg tttaactgca agccgtgctg
    13321 ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg tcacgggatt caccgtgaac
    13381 atgttcaaat agcaacagtg acgtggcgcg gccacggtcg aacagttcgc tgtactgggc
    13441 ctgggcatcg agcggatcca tcgccgttaa atcgcgcagg aaaatgccca gcgcatgggc
    13501 atcttctttc gacagatttt tcgacgcagc aatcgcctca aacatctctt gttgatgctg
    13561 ccataaggca gcatccggat attcaaggag acgcgataca atcacgagtt cgatcatgga
    13621 tgcggctccg ttttgctggt cacatcgatg gcatcgatac gacggctgtt gaacagattg
    13681 aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa agccgcagcc atttttctcc
    13741 gggaaggctt cccgtgccag ttcacgatga ctactcggca ccacaaagcg atcttcgtag
    13801 ttagcaatcg ccagataacg gtacatctcc tgtgcctggg cttcggtcag accgacctct
    13861 tccagcgcac gggtatcaac tttaccgtca acggtttcag cacgtttgta atgacgcatc
    13921 gccaacatac gtttcagtgc gcgcagtacc ggtttggtat caccggcggt cagcagattc
    13981 gccagatact gtaccggaat acgcaagctt tcgacgtctg gcagaatgcc gttgctaccc
    14041 agctcacccg cgtctgctgc agactgaatc ggagacagag gcggcacgta ccagaccatc
    14101 ggcagtgtgc gatattctgg atgcagcggc agcgccagtt tccattccat tgccatttta
    14161 taaaccggcg actgctgtgc ggcttcaata acgctcagcg gaataccgtc tttaatcgcc
    14221 tgctcgatga ctttcggatc gttcggatcg aggaacacct ccagctgacg ctggtaaaga
    14281 tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta cagcagcacg
    14341 ccaagataac ggatacgacc gacacaggtt tctgagcaca cggtcggctg acccgcttca
    14401 atacgcggat agcagaagat gcacttctca gacttaccgc tcttccagtt gaagtagatt
    14461 tttttgtacg ggcatccggt gatgcacata cgccagccac ggcatttatc ctggtcgatc
    14521 aggacgatgc catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat
    14581 gccgggttca ggcagtgttc gcacaggcgc ggcaaataca tcataaaggt gttttcgaac
    14641 tggctataca tcgccttctg gatgttgtcg aagttcttgt ctttcgccag tttgtcaaac
    14701 tcaccaccca ggtcatcttc ccagttcggc cctttttcga ttttcgccat ccgttccccg
    14761 gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg cgcggtatgc
    14821 aggttctgat agtcaaaatc gaacggctcg taataatcgt cgatccccgg cagatgcggg
    14881 ttagcgaaga ttttacccag caacatggca cggttaccca tgcgcggctg cagtttgccg
    14941 ttgattttac ggatccagcc gcctttgtat ttttcctggt tttcccagtc agtcgggaag
    15001 ccctgacccg gcttggtttc cacgttgttg aaccacgcgt attccacgcc ttcacggctg
    15061 gtccagacgt ttttacaggt aactgaacag gtgtggcagc cgatgcactt atcgagattc
    15121 agcaccatgc cgacttgtga acgaattttc attttacgct ctcctgtacc tggtcattgc
    15181 cttcgccatc taaccagtca atgttcttca tcttacgcac tacaacaaac tcatcgcggt
    15241 tagaacctac ggtgccgtag tagttaaagc cgtatgccag atgggcatag ccgccgatca
    15301 tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg gataccacca cgctgttggg
    15361 taatttccga accaggcagg ttaacgatac gttcctgcgc gtggtacatc atggtcatcc
    15421 ctgccggaac acgctggctg acaaccgcac gggcagtcag agcaccgttg ctgttgaaga
    15481 cttcaatcca gtcgttatcg gcgataccca gatctttggc atcggcttca ctcaaccaga
    15541 ccaccggacc accgcgacct aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga
    15601 taccccactt ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg tcgttggatt
    15661 tctggcccat cacttctttc accgaacggg tgtcgatcgg cggacgataa accagcaggc
    15721 tttcaccgaa atcacgcatc cattggtgat cctgatacag ctgctgacgg ccagagagcg
    15781 tacgccacgg gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat
    15841 cttcaaggcc agaccaggtc gggctggaga tgattttgcg cggctgtgcc tgaatatcgc
    15901 ggaagcgaat cttctcgtct tctttattca gcgccagatg cgtatggtca cgaccggtaa
    15961 actcgctcag ggcagcccag gctttcacgg ctacctgacc gttggtttcc ggtgccagcg
    16021 tcaggatcat ctctgccgca tcaattgcgg tgttcagcat cggctggcct ttcgccggac
    16081 cttccgcttt ggtgtagttg agcttacgca gcagatccat ctcgctctgg gtgttccagg
    16141 caatcccttt accgccatta ccgattttct ccatcagcgg gccgatagag gtaaagcgtt
    16201 cgtaagtcgc cggataatcg cgctctacga ccataatgtg tggcgcggtt ttaccaggga
    16261 tcaggtcgca ctcgcctttt ttccagtctt tcacatccag cggctgcgcc agttcagcgg
    16321 cagagtcgtg ctggataggc agcgtgacga tgtcggtttc tttacccaga tgaccgacgc
    16381 acacttcgga gaatttcttc gcgatggctt tgtaaatttc ccagtcgctt ttcgcttccc
    16441 aggccggatc gaccgccgca gacagcgggt gaataaacgg atgcatatcc gaagtattca
    16501 tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc
    16561 tcgacagacg gaagtccagc gtaaccacca gatccagctt gccttccaga ccattgtcct
    16621 gccagtccac ttcttccggc ttcacgccgc cctgttgccc cagatcttta ccctggatac
    16681 cgtgctccgt ccccagcagg tacttgagca taaactcatg acctttaccg gaagaaccga
    16741 gcaggttaga acgccagatg aacaggttgc gcgggtggtt tttaccgttt tctggttgtt
    16801 ctgccgcaaa acggatggaa ccctctttca gggatttcac cgtatagtcc accggattca
    16861 tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac tgcggtgcag
    16921 acggcagcca gcccatgcgt tccgcacgga cgttaaagtc gatcaagtgt ccggtatagc
    16981 gggatttgtc cgccatcggt gacagcaact cttccgccgt gacggtttca taacgccact
    17041 ggctggagtg gttatagaaa taagaagtgc tgttcatgtg acgcgccgga cgctgccagt
    17101 caagggcaaa cgccagcggc tgccagccgg tttgcggacg cagtttttcc tgacctacat
    17161 agtgcgccca gccgcccccg ctctgaccga cacagccgca gaaaatcagc atgttgatca
    17221 gaccacgata gttcatatcg aggtgatacc agtggttcag ccccgcaccg acgataatca
    17281 tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc acgggcgatg cgaataatct
    17341 ggctgcgaga aacgccggta atctgctcgg cccaggccgg ggtataagct ttcacatcgt
    17401 catagctggt tgcacagtta acgtcgttca ggccacgttc cagaccgtag tttgccagcg
    17461 tcagatcata aacggtggtc accagggcgg tgctgccatc agccagttgc aggcgtttca
    17521 ccggcagttt gtgcagcagc acgttttcca gttccacttt gttgaagtgt tccgtgccgt
    17581 cgccaccaaa gtacgggaaa cctacctcag cgatctcatc ctggctaccc agcaggctca
    17641 gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc catttgccct
    17701 tctcgcccca gcggaagcca atagaaccgt tcggcgcaac catttcgcca ttggtattaa
    17761 aggcgacagt tttccattcc ggattgtttt cctggcccag cgcatcaacc agatcagcag
    17821 cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc ttccagcatc accagcatcg
    17881 gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg gcttgggttg tcgaggtgga
    17941 attcacgcag cattacgtgg cccatcgcca gcgccattgc cgcatcggtg ccctgtttcg
    18001 gtgccagcca cagatcgcac agtttggcga tttcagcgta gtctggtgtg acggcaacag
    18061 ttttggtccc tttgtaacgc acttcagtaa agaagtgagc atccggggta cgcgtctgcg
    18121 gcacgtttga cccccaggcg atgatgtagc tggagttgta ccagtcagca gattccggta
    18181 cgtcagtttg ctcgccccag gtttgcggag acgcaggagg caagtcgcag taccagtcgt
    18241 agaagcttaa gcaagtaccg ccaatcagcg agagatagcg tgcacccgat gcgtaagaaa
    18301 ccatcgacat tgccggaatt ggcgagaagc cagcgacacg gtccgggccg tagtttttga
    18361 tggtgtaaac gttagatgcg gcgatcagtt cgttcacttc ctgccaggaa gaacgaacaa
    18421 aaccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag
    18481 aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac attttcatca
    18541 ggcgtttgcg catcatcggg tatttcaggc ggttggcgct gtaaagatac caggagtagc
    18601 tggcaccgcg cgggcagccg cgaggttcat ggtttggcag atccggacgg gtacgcggat
    18661 agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt ttccagctgc
    18721 aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc tgccaacgct
    18781 ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc ccatcggcaa
    18841 aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc atcggttttc
    18901 tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta agggcaaggt
    18961 aacgctctga aacagcgtgg gaattgataa cgatcaagtg tgatggggat gaaaaataaa
    19021 gtaaattcct aagatacccc tttaggataa ttaacagtta aagcataaca tgctgtaaat
    19081 taagacgttt tatacctgta gttattt
//
LOCUS       DAPPNJ010000082        19079 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000082 DAPPNJ010000000
VERSION     DAPPNJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.082"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            5..2389
                     /gene="gcd"
                     /locus_tag="RPM73_002358"
     CDS             5..2389
                     /gene="gcd"
                     /locus_tag="RPM73_002358"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDX6520708.1"
                     /translation="MNNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIA
                     GLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGIWL
                     ILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAISP
                     VADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTP
                     IKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETA
                     SPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYEPT
                     SPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEH
                     TFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTGK
                     LAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELVVP
                     APEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQMRY
                     EGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNPM
                     EQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEVVW
                     KKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSNGE
                     KLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2436..3986)
                     /gene="cueO"
                     /locus_tag="RPM73_002359"
     CDS             complement(2436..3986)
                     /gene="cueO"
                     /locus_tag="RPM73_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDX6520709.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4152..4499
                     /locus_tag="RPM73_002360"
     CDS             4152..4499
                     /locus_tag="RPM73_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDX6520710.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4605..5471
                     /gene="speE"
                     /locus_tag="RPM73_002361"
     CDS             4605..5471
                     /gene="speE"
                     /locus_tag="RPM73_002361"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDX6520711.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5487..6281
                     /gene="speD"
                     /locus_tag="RPM73_002362"
     CDS             5487..6281
                     /gene="speD"
                     /locus_tag="RPM73_002362"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDX6520712.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6319..6681)
                     /locus_tag="RPM73_002363"
     CDS             complement(6319..6681)
                     /locus_tag="RPM73_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDX6520713.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6856..9453)
                     /gene="acnB"
                     /locus_tag="RPM73_002364"
     CDS             complement(6856..9453)
                     /gene="acnB"
                     /locus_tag="RPM73_002364"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDX6520714.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9808..11565
                     /locus_tag="RPM73_002365"
     CDS             9808..11565
                     /locus_tag="RPM73_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDX6520715.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(11636..13060)
                     /gene="lpdA"
                     /locus_tag="RPM73_002366"
     CDS             complement(11636..13060)
                     /gene="lpdA"
                     /locus_tag="RPM73_002366"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDX6520716.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13385..15277)
                     /gene="aceF"
                     /locus_tag="RPM73_002367"
     CDS             complement(13385..15277)
                     /gene="aceF"
                     /locus_tag="RPM73_002367"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDX6520717.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15292..17955)
                     /gene="aceE"
                     /locus_tag="RPM73_002368"
     CDS             complement(15292..17955)
                     /gene="aceE"
                     /locus_tag="RPM73_002368"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDX6520718.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18116..18880)
                     /gene="pdhR"
                     /locus_tag="RPM73_002369"
     CDS             complement(18116..18880)
                     /gene="pdhR"
                     /locus_tag="RPM73_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDX6520719.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4378 a         5303 c         4789 g         4609 t
ORIGIN      
        1 ggcaattaac aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc
       61 gctttgcggg ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg
      121 gtactaccct atcgctggtc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa
      181 acgtgccgcg ctttggctgt acgcagccct gctgctcggc accatgattt ggggcgtctg
      241 ggaagttggt ttcgacttct gggcgctgac tccgcgcagc gacattctgg tcttcttcgg
      301 catctggctg atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt
      361 tgccgcactg gtggtggcac tactgattag cggtggtatc ctgacctggg ccggatttaa
      421 cgatccgcag gaaatcaacg gcaccttaag cgccaatgcc acacctgcgg aagctatctc
      481 acccgttgcc gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc
      541 acctctgaaa caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg
      601 tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat
      661 taaagtgggc gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc
      721 cgccagcggc aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca
      781 gcacgtaacc tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga
      841 agtgatggcg gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc
      901 gattaacgct gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct
      961 gcaaagcaat atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat
     1021 caccgataaa accatcgtga tggccggttc agttaccgat aacttttcaa cccgcgaaac
     1081 gtctggcgtg atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc
     1141 cggcgcgaaa gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc
     1201 aaactcgtgg gcaccagcgg cctatgacgc gaagctggat ctggtgtatc tgccgatggg
     1261 cgtgaccacg ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag
     1321 ctcgattctg gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca
     1381 ccacgacctg tgggatatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa
     1441 tggtcagaaa gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga
     1501 tcgtcgtaat ggcgaactgg tggttccggc accggaaaaa ccggttcccc aaggtgctgc
     1561 caaaggcgat tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa
     1621 agatttgagc ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt
     1681 gatgttccac cagatgcgct atgaaggcat tttcaccccg ccatctgaac agggtacgct
     1741 ggtcttcccg ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg
     1801 tgaagtggcg attgccaacc caatggcact gccgtttgtt tcgaaactga tcccgcgtgg
     1861 tcctggtaac ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat
     1921 tcagccacag tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg
     1981 tctgccgtgt aaacagccag catggggtta tatctcggcg ctggatctga aaaccaatga
     2041 agtggtgtgg aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt
     2101 accggtgccg ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa
     2161 cgtgctgttt atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg
     2221 tgaaaaactg tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga
     2281 agtgaatggc aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa
     2341 gatgggcgac tatattgtgg cttatgcgct gccggatgat gtgaagtaac cataatgccc
     2401 ggagagatcc gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc
     2461 cccgtatctt catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgca
     2521 tcgtgattaa acttcaccag cacttcgctg acattacctt ctaccttaac ggtatctttc
     2581 cagcccgcgc gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg
     2641 ccgtggatat ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg
     2701 tattgccctt tcgccgccgc aaacatcggc ttgttcatat caaacgcctg accgttgatt
     2761 ttattggcat ggtggaaatc gaacttcccg ccgtggttca tatgattcat attaccgtgc
     2821 cccatatggc ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc
     2881 tccattagca tctgcatacc catcatatcg agcatcgggt ccatagaaag ttgcagcttg
     2941 cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa
     3001 gcaccggagg cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac
     3061 ggcgcaatcg ccatccccat ctggctgacc ggtagcgtca ccaggtcaaa gggtttgttg
     3121 tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc
     3181 ttcaccggtt caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg
     3241 ctggtggcga aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa
     3301 ccacgcgggg cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac
     3361 cagcccacgg cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat
     3421 tttttatcct gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat
     3481 ttcaggatct cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc
     3541 ccggtcttgc catgttgatg tggatggaac cagcaagtag cggcaggttg atcaacgttc
     3601 aacgtcaccg agcgcttgcc acctggcgga ataattccct gcgggccgcc gtcgacttca
     3661 cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt ccgtcagttg gttatagata
     3721 tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta
     3781 tagccccagg tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac
     3841 tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc
     3901 gccgcaaaga ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa
     3961 tattttaaga aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa
     4021 atcaaacgtt gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa
     4081 tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc
     4141 tggtagaagc aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt
     4201 gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag
     4261 tttttgtctt tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc
     4321 gcgcactggt ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg
     4381 acatgaaagc tctcggtgaa gacagctacc gcgatctcag cggcattggc attcctgtcg
     4441 ctaaaaaatg caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat
     4501 ccctgatacc tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg
     4561 ttgcgccctt tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac
     4621 agtggcatga aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt
     4681 atcatgaaaa gaccgatcac caggatctga tcatttttga gaacgctgca tttggtcgcg
     4741 taatggcgct ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga
     4801 tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg
     4861 gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga
     4921 tggtggaaat cgatgcgggt gtcgtatcgt tctgccgtca gtatctaccc aaccataacg
     4981 ccggtagcta cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta
     5041 atcaaaccag ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg
     5101 gcgaaagcct tttcacttcg gcattttatg aaggctgcaa acgttgcctg aatcctggcg
     5161 gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc
     5221 atcgcaaact cagccattac ttcagcgacg ttggctttta tcaggcggcg attccgacct
     5281 attacggcgg tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct
     5341 caaccgaaat tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc
     5401 cggcaatcca tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac
     5461 agccgtccta aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc
     5521 tgaccaaaag tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc
     5581 gcgacggtta tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc
     5641 tgtcagaaac ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg
     5701 aaccacaggg tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca
     5761 tcgacaaaac agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa
     5821 gtcatatttg cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc
     5881 gcgccgatat tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc
     5941 tgatccacca gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc
     6001 gcgacattaa cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta
     6061 tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata
     6121 tcttccatac caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca
     6181 aaccggaaga tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa
     6241 tgcgcgagat ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc
     6301 aggctccgcc agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg
     6361 cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc
     6421 ttccatttca tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc
     6481 catccacagg gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc
     6541 gtgcgctgct tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa
     6601 ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc
     6661 gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca
     6721 tcgcgccatt gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt
     6781 ttagaaataa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
     6841 gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta
     6901 ctgagaaagc tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg
     6961 cgcaacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
     7021 cgccagttct gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt
     7081 acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca
     7141 cagagaacag cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc
     7201 ttcttcagtc aactgtgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg
     7261 caactggcct ttatgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt
     7321 catgcaagaa ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg
     7381 cgcgtcatcc gggtcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat
     7441 gtcaatcact gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag
     7501 ccatttttcc atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc
     7561 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
     7621 cagcttgata gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc
     7681 ctgctcaact ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt
     7741 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc
     7801 gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
     7861 ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga
     7921 acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcacctc cggtaccgac
     7981 ggtatccggc agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag
     8041 cgacacaccg ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
     8101 tggcttcgga tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc
     8161 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc
     8221 tacagaagtc attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc
     8281 acggcctacc attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
     8341 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc
     8401 acgcccgata atcagcggga tacggccgcc agcacgcact tcatcaatca gcacgtcggt
     8461 tttcagttcg aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta
     8521 cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
     8581 tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa
     8641 accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga
     8701 gttagtggcg gatttacgcg aagaacccgt acccacaacg tcaccgacgt acgccagcgg
     8761 gaaacctttc tgttgcagag cttcgatttg cttgatcgga ccaacaacac caggctggtc
     8821 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
     8881 gcgtgaccac gcatccggtg ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt
     8941 gaagacggta acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc
     9001 cgcccaggac tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac
     9061 gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc
     9121 cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt
     9181 gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttcgc
     9241 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg
     9301 gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
     9361 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
     9421 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
     9481 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
     9541 agtaccgcaa cgggcagaat agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag
     9601 caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
     9661 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
     9721 tttaaactac cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac
     9781 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
     9841 ctggcgctaa tttgcagtgc cgggctttgt gccgcctcta ccgggctata tataaaaagc
     9901 cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc
     9961 gcagttacgc ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc
    10021 gcattcagca ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct
    10081 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa
    10141 tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcagt atctgaccag
    10201 ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc
    10261 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    10321 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca
    10381 accgaacaga aagttattac cacaacgaag aaagctgtac cggtaacaca gacagtcacg
    10441 gctcccgtca taccatccaa taccgtttca actgccagcc ccatcattac agagcctgca
    10501 acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac
    10561 ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca
    10621 ccagccggag aaccgtttat tgacagcttt gtgcgcggat tcggctatag catgggtgtt
    10681 gctaccacgt acgcactatt cagcagcatc gactgggacg acgacgatca tgaccatcat
    10741 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg
    10801 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    10861 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    10921 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt
    10981 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    11041 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    11101 cagcggttta atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt
    11161 cgccaacccc tgacccagca acaaaaggac gccgctcgtc agcgttatca gtccgcctcg
    11221 cctgagcagc gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga
    11281 agagaggcag cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtatttaag
    11341 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata
    11401 caatcggcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    11461 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta
    11521 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    11581 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact
    11641 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    11701 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    11761 cacaacccat ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga
    11821 caatcgcacc accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    11881 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    11941 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    12001 cggtataggc gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa
    12061 cttcagcggc aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga
    12121 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    12181 aaccacggtc gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    12241 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    12301 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    12361 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    12421 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    12481 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    12541 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    12601 acggcagttg gatcgggcga gaacccgctg caatgatcgc gttgtcgaag ttgatcacgg
    12661 ttttaccgtt ctcaccttca acttccaggg tgttagcccc ggtaaattta cccagaccgt
    12721 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    12781 tcactttctc tttccaggta cgaatcttgt caatatcggt tttcggttcg ccgaagacga
    12841 taccgtgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    12901 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    12961 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    13021 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    13081 ctccggcggt catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc
    13141 ccagtcactt acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca
    13201 gaaatccata gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg
    13261 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    13321 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    13381 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    13441 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    13501 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaaccccga
    13561 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    13621 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    13681 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    13741 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    13801 atttcttcag ggtcagacgc tgaccgtctt ccgacaacga actgttgaag cgaggcatct
    13861 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    13921 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    13981 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    14041 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    14101 ccaccttcgg ccacggcagc atgcctggga taccgccgcc agtcgccgcc ggagctgctt
    14161 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    14221 tacggccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    14281 tcagcggagt cgcgtgaacg taagcgtcgt tttctgcaaa ttcagatttg ccttccgctt
    14341 tcgcagccgg tgctgctgcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    14401 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    14461 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    14521 gagccggaac ttccatagaa gctttgtcgc cttctacggt aatcagtgac tgttcagcgg
    14581 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    14641 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    14701 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    14761 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    14821 acggagccgg aacttccata gacgctttat cgccttctac ggtgatcagc gactgttcag
    14881 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    14941 tatccggaac gttaacgtct tttgccgctg cagccgctgg tgctgctgcc ggagctgctt
    15001 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    15061 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    15121 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    15181 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    15241 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    15301 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    15361 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gctacaacca cgtaggaagc
    15421 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    15481 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    15541 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    15601 gcgcggagtt tccagcgggt gcagcatgtt ccagcgttca caatcctgac catctcgcgc
    15661 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    15721 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
    15781 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    15841 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    15901 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    15961 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    16021 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    16081 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    16141 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
    16201 agaggtcgcc gctgccagcc aggaacaacc tgcgcccaac tcgttgatcc cttcctgcag
    16261 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    16321 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    16381 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    16441 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    16501 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    16561 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
    16621 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    16681 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    16741 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    16801 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    16861 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    16921 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    16981 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    17041 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    17101 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    17161 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    17221 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    17281 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    17341 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
    17401 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    17461 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
    17521 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    17581 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    17641 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    17701 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    17761 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    17821 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    17881 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    17941 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    18001 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    18061 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    18121 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    18181 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    18241 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    18301 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    18361 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    18421 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    18481 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    18541 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
    18601 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    18661 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    18721 cctcacgcaa ggagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
    18781 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    18841 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    18901 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    18961 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    19021 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaag
//
LOCUS       DAPPNJ010000083        19035 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000083 DAPPNJ010000000
VERSION     DAPPNJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.083"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            89..1075
                     /locus_tag="RPM73_002370"
     CDS             89..1075
                     /locus_tag="RPM73_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDX6520720.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            1090..1431
                     /locus_tag="RPM73_002371"
     CDS             1090..1431
                     /locus_tag="RPM73_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDX6520721.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            1436..3082
                     /gene="fliF"
                     /locus_tag="RPM73_002372"
     CDS             1436..3082
                     /gene="fliF"
                     /locus_tag="RPM73_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDX6520722.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            3060..4070
                     /locus_tag="RPM73_002373"
     CDS             3060..4070
                     /locus_tag="RPM73_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDX6520723.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            4073..4783
                     /locus_tag="RPM73_002374"
     CDS             4073..4783
                     /locus_tag="RPM73_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HDX6520724.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPV
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            4776..6113
                     /gene="fliI"
                     /locus_tag="RPM73_002375"
     CDS             4776..6113
                     /gene="fliI"
                     /locus_tag="RPM73_002375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDX6520725.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPCAKKAEQ"
     gene            6116..6550
                     /gene="fliJ"
                     /locus_tag="RPM73_002376"
     CDS             6116..6550
                     /gene="fliJ"
                     /locus_tag="RPM73_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDX6520726.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLVE"
     gene            6553..6948
                     /locus_tag="RPM73_002377"
     CDS             6553..6948
                     /locus_tag="RPM73_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HDX6520727.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(7066..9510)
                     /locus_tag="RPM73_002378"
     CDS             complement(7066..9510)
                     /locus_tag="RPM73_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX6520728.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQIVLLNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRCYPQLAQQMSNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKITDN
                     ISYYFNKLPVATKPSTLPIADTIGFFMETSFHYTLYRPIITALQAQGQSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(9689..10618)
                     /locus_tag="RPM73_002379"
     CDS             complement(9689..10618)
                     /locus_tag="RPM73_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HDX6520729.1"
                     /translation="MSFIECYEPEYVRNFMTQHPGSPLYVRKVWKNEIRLSLMLTEPE
                     SCKAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSRASKMPGRDGDILARLTTLPLALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDVIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            complement(10745..11173)
                     /locus_tag="RPM73_002380"
     CDS             complement(10745..11173)
                     /locus_tag="RPM73_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDX6520730.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRERLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQCEFIQSFLGSEADFLYQE"
     gene            complement(11186..11464)
                     /gene="flgM"
                     /locus_tag="RPM73_002381"
     CDS             complement(11186..11464)
                     /gene="flgM"
                     /locus_tag="RPM73_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDX6520731.1"
                     /translation="MKITPTMPGNRPTATTAGSNQPRKTTATTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            complement(11545..12282)
                     /gene="flgA"
                     /locus_tag="RPM73_002382"
     CDS             complement(11545..12282)
                     /gene="flgA"
                     /locus_tag="RPM73_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDX6520732.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMVLDDAI
                     GFSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            12364..12699
                     /gene="flgB"
                     /locus_tag="RPM73_002383"
     CDS             12364..12699
                     /gene="flgB"
                     /locus_tag="RPM73_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDX6520733.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            12702..13133
                     /gene="flgC"
                     /locus_tag="RPM73_002384"
     CDS             12702..13133
                     /gene="flgC"
                     /locus_tag="RPM73_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDX6520734.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDSASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            13133..13846
                     /locus_tag="RPM73_002385"
     CDS             13133..13846
                     /locus_tag="RPM73_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HDX6520735.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMGVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPQGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            13931..15133
                     /gene="flgE"
                     /locus_tag="RPM73_002386"
     CDS             13931..15133
                     /gene="flgE"
                     /locus_tag="RPM73_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HDX6520736.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENCLEAVSGTAWVQT
                     GESGTPLIGVPGSGACGTLTSGVLESSNVDITSELVNLMTAQRNYQANTKVISTSTQL
                     DDALFQAM"
     gene            15133..15870
                     /locus_tag="RPM73_002387"
     CDS             15133..15870
                     /locus_tag="RPM73_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HDX6520737.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            16049..16834
                     /gene="flgG"
                     /locus_tag="RPM73_002388"
     CDS             16049..16834
                     /gene="flgG"
                     /locus_tag="RPM73_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDX6520738.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            16917..17582
                     /gene="flgH"
                     /locus_tag="RPM73_002389"
     CDS             16917..17582
                     /gene="flgH"
                     /locus_tag="RPM73_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDX6520739.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGESTVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            17597..18697
                     /locus_tag="RPM73_002390"
     CDS             17597..18697
                     /locus_tag="RPM73_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDX6520740.1"
                     /translation="MQNWIKAAAIAVSLMLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVVLHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            18697..18996
                     /locus_tag="RPM73_002391"
     CDS             18697..18996
                     /locus_tag="RPM73_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HDX6520741.1"
                     /translation="MKLNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
BASE COUNT         4360 a         5201 c         5614 g         3860 t
ORIGIN      
        1 tattatcgtt taattcgaac ttgttaatcc cgccggtgat acgcttcaga acatcctgat
       61 tattgtcgct acttatatcg ttcaccctat gtctgaactt attgcaactg ccgcttccag
      121 cattaacgct tttactctgg caaagcgtgt cgccgcattt aacgtgccgg tgctcattca
      181 gggcgaaacc ggcgcgggca aagagtgcgt ggcgaaatat attcacacag tcgcctttgg
      241 tgaaaatgaa aacgcgccct atatcggcgt gaactgcgct gcgatcccgg aaaacatgct
      301 ggaagcaacc ttatttggct acgacaaagg cgcatttacc ggcgcaattg ccagtgtgcc
      361 aggaaaaatg gaactggcga ataacggcac cttattactc gatgaaattg gcgatatgcc
      421 gctggcgtta caggccaaaa tattacgcgt attgcaggaa caacaggttg agcgattagg
      481 cagcaaccga caaattaaac tcaattttcg cctgattgcc tgcaccaata aaaaccttga
      541 gcaggaagtc gccgccgggc gttttcgtga agatctctat tatcgtctgg cggttattcc
      601 tattaccatg ccgccgctgc gtgaacgtct gaacgatatt attccgctgg cagagtcatt
      661 tataaaaaaa tactccacgg tgctggtgaa aaatatcacc ctttcagaat ctacccgccg
      721 tgcgctgctg aattaccgct ggcccggcaa tgtgcgccag ctggagaacg ccattcagcg
      781 gggaatgatt ttaaatcgcg acggcgtgat ttacccggac gccttaggcc tgccggaaaa
      841 tgacattgcc gaccacagcg aactgcaatg gcctgctcag cccgccgtac acattgccga
      901 aaccagcgat ttaggccagc acggacgaag cgcccagtat caatatatcg ctgacctgat
      961 gcgtaaatat cagggcaacc gcagcaaaat cgccgacctg ttaggtatta ccccgcgcgc
     1021 actgcgctat cgactggcct ccatgcgcaa gcacggtatc gaagttttct cctgacccgg
     1081 aagttaaaaa tgagcattac cacaatcaac agcaccatgc aggcgcagat catgcaggac
     1141 gtgcagcgga tgcaggccaa cgcccaggcg ccggtgctgc ccgccatgac cttcagttcc
     1201 accgacccgg acgtttcgtt tcaccgcatt atgcagggcg cgctcggtca cgttgaccag
     1261 ttccagcagg tggcggagca gcagcaaacg gctatcgaca tgggcaaaag cgatgatctg
     1321 gcaggtgcga tgatcgccag ccagcaggcg tcgctctctt tctccgcgct ggtgcaggtg
     1381 cgcaacaaaa tcgccaccgg atttaacgat ctgatgagta tgtcgatcta accctatgaa
     1441 tgcacaaata aagaagttaa cccaggcgtt tccggcgttt cgactgcgtc tcgcagataa
     1501 caaacgctgg gcgctgatgg cgggtgtggg gcttgccgtg gcggcgacgg cgattatcgt
     1561 tagcgtgctg tggaccggca atcgcggcta cgtctcgctc tacggacgcc aggaaaatct
     1621 gcccgtgtcg cagattgtca ccgtgctgga cggcgaaaag ctcacctacc gcattgaccc
     1681 gcaaagcggg cagatactgg tgccggaaga ggcgctgtcg aaaacgcgca tgacccttgc
     1741 cgccaaaggt gtgcaggcga ttttgcccag cggctacgag ctgatggaca aagacgaagt
     1801 gcttggcagc agccagttcg tgcagaacgt gcgctataaa cgcagcctcg aaggggagct
     1861 ggcgcagagc attatgtcgc tggacgcggt ggagagcgcg cgggtgcatc tggcgctcaa
     1921 cgaagaaagc tcgtttgtgg tcagcgatga gccgcaaaac agcgcctcgg tggtggtgcg
     1981 tctgcactac ggcgcgaagc tgaatatgga ccaggtgaac gccattgtgc atctggtttc
     2041 cggcagcatt ccggggctgc acgccagcaa agttagcgtg gttgatcagg cgggaaatct
     2101 gctgaccgac ggcatcggcg cgggcgaggc ggtctctgcc gctacccgta aacgcgatca
     2161 gatcctcaaa gacattcagg acaaaaccca cgccagcgtg gcgaacgtgc tggattcgct
     2221 ggtcggcagc gggaattacc gtgtcagcgt gatgccggat ctcgacctca gcaccatcga
     2281 cgaaactcag gaacactacg gcgacgcgcc gaaaataaac cgcgaagaga gcgtgctgga
     2341 cagcgacacc aatcaggtgg cgatgggtgt gcccggctct ctcagcaacc gtccgccggt
     2401 tgcggcgaat caaatgacta acggtacgga agaaaatcgc tcgcctgaag cgttatccaa
     2461 acacagcgaa agcaagcgcg attactctta cgaccgtagc gtccagcata ttcagcatcc
     2521 cggctttgcg gtgaaacgcc tcaatgtggc ggtggtgctc aatcaaaacg ctccggcgct
     2581 gaaaaactgg aagccggagc agaccgcgca gcttaccgcg ttgctgaaca atgccgccgg
     2641 tattgacgcg caacgtgggg ataacctgac gctttcactg cttaactttg tgccgcaggc
     2701 ggtgccggtc gaaccaatta ttccgctgtg gaaagatgac agcgtgctgg cctgggtgcg
     2761 cctgattggc tgcggcctgc tggcgctgtt gttgctgttc ttcgtggtgc gcccggtaat
     2821 gaaacggctg acggcggtac gtgcaccgat tatcacacca gaatcggaag ccgtcagcga
     2881 accgtggatt gccatgccgg aagaggagcg caaaaacgtc gatctgccgt cgctgcccgg
     2941 cgatgacagc ctgccgtctc agagttccgg cctggaagta aaactcgagt tcctgcaaaa
     3001 actggccatg agcgacaccg atcgcgtagc cgaagttctc agacaatgga tcaccagcaa
     3061 tgagcgaatt gacaacaaat aagagcaaca gctacctgga acaggcggcg atcctgctgc
     3121 tgtgtctggg cgaagaggcg gcggcgacgg tgatgcaaaa actcagccgc gaagaagtgg
     3181 tgcgcctgag cgaaaacatg gcacggcttt ccggcgtgaa aaccagcatg gcgcgcaaag
     3241 tgatcaataa cttctttgat gagttccgcg aacagagcgg gatcaacggc gcatcgcgat
     3301 ccatgttgca gggtattctc aacaaagccc tcggcaccga gatcgccagc agtgtgatca
     3361 acggcatcta cggcgatgag atccgctcgc ggatggcgcg cctgcaatgg gtggaaccac
     3421 gccagctggc gatgctgatc tccgaagagc atttgcaatt acaggcggtg ttcctcgcgt
     3481 ttctgacgcc ggaaatctcg gcggcggtgc tctcgtatct caatgaatcg gtgcaaaacg
     3541 aaattctcta ccgggtggcg aaacttaacg acgttaaccg cgacgtggtg gatgaactgg
     3601 acaggctgat cgaacgtggc ctgtcggtac tttcggaaca tggctcgaag gtgaaaggca
     3661 ttaaacaggc ggcggatatc gtcaaccgct tccagggcaa ccagcaggtg atcctcgacc
     3721 agatgcgcga gcgcgacgaa gaagtgctgg agcagctcca ggatgagatg tacgacttct
     3781 ttatcctcag ccgccagaac gaagaggtgc gtcgacgtct gcttgacgaa gtgccgatgg
     3841 aagactgggc ggtggcgctg aaaggcaccg aagccctgct gcgccgttcg atttacgcgg
     3901 taatgccaaa acggcaggcg cagcaactgg aagccatcac cgcgcgtctg gggccggtgc
     3961 cggtcagccg tatcgaacag atccgccgcg aaatcatggg catcgcccgt gaactggaag
     4021 aggcgggcga aattcagcta cagctctttg cggaacagac ggcggagtaa ccatgaccat
     4081 tgaaacactg cgcgggcgct atcgcctgca ccgtttcccg ccgcgccagc gtgcggtgac
     4141 cggagaaccg ctcactacca gtattagcgc cgccgattac cagcgccagt tgatggacgg
     4201 atttcaggaa gggctgcaaa aagggtttgc tcagggaatg accgaaggcc aggagcaggg
     4261 ctttagcgaa ggtcatcagc aaggttttgc cgaggggcga cgccagggtt acaccgaagg
     4321 cagcctcgcc gggcagcagg aagggcgcaa acagtttgtt gaggcggcgc agccgctgga
     4381 agccatcacc ggtaaagtga acgacttcct cgctcatatc gaacgtaaac agcgggaaga
     4441 tttgctgcaa ctggtggaaa aagtcacccg ccaggtgatc cgttgtgaac tggcgctgca
     4501 acccacgcag ttgctggcgc tggtggaaga ggcgctggcg gccttcccgg tgatgccgga
     4561 aaccctgcaa gtgatgctga gtacggaaga gtttaaccgc ctgcgcgacg cggtgccgga
     4621 aaaggtcagc gaatgggggc tgacgccttc gccggatctg ccaccgggcg aatgccgggt
     4681 gatcaccgac aaatccgaac tggatatcgg ctgcgagcat cgccttgaac aatgcatgac
     4741 cgcgctgaaa gaaacactca cgccggagtc gcagggtgag tgatttttct gcgttcgata
     4801 acgccctgcg ctcgctggag tcgatcccgc tggcccgcgt cgccgggcgg ctggtgcgcc
     4861 ttaacggcat cctgctggag agtgtcggct gcccgctaat gaccgggcag ctgtgccgca
     4921 tcgaaagtgc caatcacacg ctgatcgacg cgcaggccgt gggctttaat cgcgacatca
     4981 cctatttaat gccgtttaaa cagccggtcg gcctgatggc aggcgcacgc gtcttcccgg
     5041 aagaaaaagc ccacgacatc ctgataggcg aaagctggtt aggccgcgtg gtgaatggcc
     5101 tcggcgaacc gctggatggc aaaggccgcc tgaacggcaa cgaccttctg ccgccgttgc
     5161 cgccctcggt taatccctta actcgtcgtt cggtagacga accgctggac gtgggcgtga
     5221 aagcgatcaa cggcctgctg accatcggca aaggccagcg cgtcggacta atggcaggca
     5281 gcggcgtcgg gaaaagcgtg ctgctgggga tgatcacccg ccagaccaaa gccgatatcg
     5341 tggtggttgg gctgattggg gaacgcgggc gcgaagtgaa agagtttatc gaccactcgc
     5401 tgggcgcgga cgggctggca aagtctatcg tcgtcgtcgc gcctgccgat gaatcgccgc
     5461 tgatgcgtct gaaagccacc gaactgtgcc actccatcgc cgcctggttt cgcgaccgcg
     5521 ggcatcacgt cttactgctg gtggattccc tgacccgcta cgcgatggcg cagcgggaaa
     5581 tcgccctctc gctcggcgaa ccgcccgcca ccaaaggtta tccgccatcg gcattcggca
     5641 tgatccccaa actggtcgaa agcgcaggca acagcgaaag cgcgggatcg atgaccgcca
     5701 tctacaccgt gctggcggaa ggtgacgacc agcaagatcc gattgtcgac tgcgcgcgcg
     5761 cggtgctgga cgggcatatc gtattaaccc gcaaactggc ggaagcgggg cactatccgg
     5821 ccattgatat cggccagtcg atcagccgct gcatgaacca ggtcaccagc ctggaacatc
     5881 agcaatccgc ccgcgcgtta aagcaaaact atgccgccta tatggagatc aaacctctga
     5941 tcccgctcgg cggctatgtg gcaggtgccg acgccagcgt cgacaaagcg gtgaaaatgt
     6001 ttccggccat tgagcgattt ttgcgccagg agatgcgcga acccgcctcc ctcgaactgg
     6061 tgcaaagccg cctgcaaatc ctctttcctt gtgccaaaaa ggcagaacag taactatgcg
     6121 acaaatcatc gacaccctgg cgcagttgca gcgcctgcgg gataaatcgg tcaaagacaa
     6181 aaccgtcgag ctggcaaagc aaaaacagat ttgcgcgggt tatgacaaca atatcaaggc
     6241 gttaggctac cttgtcgaga aaacatctgc gggtgcggcg gtgtcggtgg aatcgctgaa
     6301 aaacgtcagc ggctacaaag gcaccctgcg caaggtaatc gcctggcagg agcaggaaaa
     6361 aacgctggcc aatatcaaag ccacgcgaat gcagaaaaac ctcacggcgg cggcgtgcga
     6421 agagaaagta gtcgccctga cgctggacga caaacgccgc gagcaacaag aaagcgccac
     6481 ggcgaaagcg caaaaagcgg tggacgacat cgccgtgcag tgctggctgc ggcacaaatt
     6541 agtggagtag ccatgaaaac ggtcatcacc ttcggcacct tcgatgtgtt tcatgtcggt
     6601 catctgcgcc ttttacaacg cgcccgcgca ttgggcgagc ggctgctggt cggcgtatcg
     6661 tccgacgccc tgaacatcgc caaaaagggc agggcaccgg tttaccatca ggatgaccgc
     6721 atggcgatca tcgccgggct ggcgtgcgtg gatggcgtat ttctggaaga gtcgctggag
     6781 caaaaagcgg agtatttgcg cgggtacagt gcggatattt tagtgatggg ggatgactgg
     6841 gcagggaagt ttgacagctt tgcgtatatt tgtgaggtgg tctatttccc ccgcacgccg
     6901 tcggtgtcga cgacggggat tattgaggtg attagagggc ttttataaag tactaaaaca
     6961 gagatgtatt ttcttgccgg atgcggcgta aacgccttat ccggcctaca ggacgtgatc
     7021 gatccgtagg cctgataagc gaagcgcatc aggcagttgc gcggtttacc ccagcgcctc
     7081 cacaatcaca atcgccgccc gttctcccgc cttgccatcc ataaacgcat cgcaatagtg
     7141 atggcgaatc tccgtaagcc gcgcctgcac cgacccccag tcaaacgcct ctgacaacaa
     7201 ataacgcagt tctgcaacat catgagcaac cggcaatatt tcgcggattc gctggtcggc
     7261 gctctctgcc gtggaataac ttttctcacc gtcgagcaaa tcattcatgc ccggaaagtc
     7321 gagcaggatc acccgcttat caacgtggat agcatcgaaa ataaacccgc tgttatcggt
     7381 cagcacgtaa tcggcttttg ccagcagcgc cagcgtgtgg cgggcggagt cggtgcgctg
     7441 ttttaaatgc ctgcgcacca gggccagcga tgctgcttct tccgggcgtg agcaggtgcc
     7501 atggtgcagt ttgcaaatca ggttcacatc accgctcaag cggcccaatt tttcagccca
     7561 gtggggcagg gagcttaatg cgccaaacgt tggggcatag agcaccgtag gtttacggta
     7621 atctgattgg atgttttccg gcaacgcgcg atcgaacgtg ccgttatgcc aggcgtcgaa
     7681 gcgcgggttg ccgaccactt ttgcgctgcc gaaatgcgcc agcgcctgct gactgtagtg
     7741 gctataacac aaaatgcttt ggtaaaaacg attccagtcg gcgtggttcc acgtctcttt
     7801 ggctaagccg tacatagcgc ggatgttcac cgccgccagg ccattgagcg caggcgtgtg
     7861 gtaggggctg accaggcatt tcacccgctg accatgtgtc tgcatttctg acagacgcat
     7921 acctttaagc tgcggatcgt cgatgttttt cagtgtttcc agcatctcat caagaaacgg
     7981 tttaaacacg cggtcattga tcactagtgt gcagctctgc ccctgcgcct gtagagcggt
     8041 gatgattggg cggtacagcg tgtagtggaa tgaggtttcc ataaaaaagc cgatggtatc
     8101 ggcgatgggt aatgtagatg gcttggtggc tacgggtaac ttattgaaat agtaactaat
     8161 attgtcggta atttttttct cgatatcact aaagcccggt cgctggtaac aaaggctcgg
     8221 gtaacaggcc agccatttgt cggtgcgtag cagcggctgc cagccgtctt ccagcgagtg
     8281 ctcatcgttg ctcatctgct gcgccagttg cgggtagcag cggctgttca ccagataagc
     8341 gcataccttg cggcagtccc gcgcgtgcac cagcccaggc aaacttttta gcatcgttcc
     8401 ctgcgaaatt tcgccaccga ggatcatcac ctgccaggga attttcgcca ggctcgccag
     8461 cagcgtgttc agtacctgaa tatgtttctc ctgcttgaga ataaccgcgt catcttccag
     8521 cagcagatag ttttgccagc cgtgctgttg cgccaggcgc agcgcctgta agtgcgactg
     8581 acggcggccc cgttgaccgt tttcatcttc actggcggca aggcgggtga tcttctcagg
     8641 tggaatatgc agcaatgcca gttcttcacg gatctgttgt aagcgatccg ggcgtttatc
     8701 cagattcagc agaacgatct gatcgatcgc ctgatggttg atcgcctggt gatgagtggc
     8761 gttatgcaga ctcagataag cattgcgcag cagcggatcg cggacgatat ccgtaagcgc
     8821 cgtgtttact ggcgtgctgg ccccgataat aaagtcttta tcaaaggtgt tgtggctatg
     8881 cgagatacac aaaatcgtgc gctcgccggg cagttgcagc gggttaacgc tgaaattgtc
     8941 ggtgaaactt ttctcttcgc ctaaattgca atcatcgtcg taacggtgct ttttcaggta
     9001 gttacggtga tagcaaaagg tgccgttgag gatattatgt gggccaaagc tgcggctttg
     9061 aaagatacgg ttgatatggc tgtaccagat ggggatctgg tcggaaccgg agatgagcgc
     9121 ccggtgtttt tgcatcatag cgatggtgta agagatttta tctgccggat agtagtcgtc
     9181 atcatccatg cagaggatgt attcgccgcg cgccagttcg ttgagcatgt tgcgtttttt
     9241 gcccagcggc agtttttccg ggtgatggat gtagcggatg ttaaaggctt ccggcgtgcc
     9301 attggtcagg cggtcgatga tgtgttgatg gctttgtggg gaatcgtcga ggatgatgag
     9361 ttcgcggcgg tctgccgggt aatcctgata gcggtacata tagagcaggt acggcaggaa
     9421 tgcgccgcgg ttccaggttg gcgtgacgac gctgacaaac ggtttttcgc ggtgggtggc
     9481 gagtgttttg atgatggttg gtgttggcat ggtgtttggg tgagttaatg tgtgaatgtg
     9541 atgaacgtgc gtgctgatgc cctcaccccg accctctccc acagggagag ggagaaaatc
     9601 agcgccgatt ttaacaccgt gctcgatcgg tcccctcgcc cctttgggga gagggttagg
     9661 gtgaggggca aagcgcgcgc agagcgactc acgttagcag cgaaaacgct gaaagcagcg
     9721 ggtcggcggc acataacgac tgaatttgct ggcgaaccaa cagcgcattt tcactggctc
     9781 gccagcgttc aaacatggca aagagggcga aaatatcctc ctgccgcagc ggagaaccat
     9841 ccagcgtcca taaactgatt aacgtgcgga ggagataaaa atcgcacacc agcgggaaat
     9901 aatccgcccc gtcagtctgc ccaatcacat cgtggtaaac gcggtaaatc agccagttac
     9961 gcatcatcca gggcgcggtg gcaaaatgct gttgccaggt ggtttgccac tgcgtctgaa
    10021 gctgctgttg taacagggct tcgctgttgg cgtcatgcag cgtcagtaaa gtaacctgca
    10081 acggcagcga ggctttgcgc gggctttccg gcaaaatcga ccagtcaaac gcacaaccgc
    10141 cgagcagcgt catcaactgg cgcgccagct gtggcacggg cgggagctgg gcgtactgtg
    10201 cctgtagcgt accttttttc gcatgatcgg ccagcgccag tggcagcgtc gtcaaacggg
    10261 ccaggatgtc tccgtctctg cccggcattt tgctggcgcg ggagagcaaa atcccgacag
    10321 catacaggga aagctccggg gttaaatcac ggagcgtgag ggtactcaaa atcacctggt
    10381 ttactatcgc atcgcgcgcg gaaagctctg cggaattatc ttccacaggg cggtaggtga
    10441 gctgtaaatc aaaggccctg acatcgttga gcaccgcttt gcaactctct ggctctgtca
    10501 gcatcaggct caggcgaatc tcgtttttcc acaccttgcg gacgtataaa ggcgaaccgg
    10561 ggtgttgcgt cataaaattg cgcacgtatt ccggttcgta acattcaata aaactcatag
    10621 gtggcttcca gtctttaatg ctgtgcagga tgtgcaataa gcgtgccggg attttttgta
    10681 gtcaaaaaca agggattggc aatcaggcgg aaaatccatt tccgctatgg ggaaggtatg
    10741 cgcgttattc ctgatagaga aaatctgctt cgctacctaa aaacgactga ataaattcac
    10801 actggcgaat caacagctcg ccgtttttgt cgttgtaggc tttgcagcgt tccgcttttt
    10861 gctcaaggcg ttgccacaac tgttgtgcag ccgctttttg aacagccgga agccaggtga
    10921 aaacctgcgc cagaccgtca cgatttacgc taacaccgag cattttcagc aggctatgcc
    10981 gctgatggct atgtccatgc aactgctcat agtgatgggt tatctcatcg tttaccgcca
    11041 gcagctcctc gctggcgcgg cgaatcatgc agatccgctg gcgctggaga cgttcgcgca
    11101 gggaatcata cagcgcgtcg tcgtcacgaa tgccctgcag caacgccttc acctgttgta
    11161 accggatact cacttcatcg gcctcttagt tctggaaaaa actcagcatg tcgctggcta
    11221 actgttcggt gtccacttgc aggctgccgg aggccagcat cgcctgcatt tgcgccactt
    11281 tgtcgctgtc gatatcgctc tcttgctggt cgttcagcgt ttgctgggcg gactgcaatc
    11341 ccgcctgagt aatgtcgtcg gcgctcagcg tcgtggtggt ggtcgccgtg gttttgcgcg
    11401 gctggttgga gcctgccgtc gtggctgtcg ggcgattgcc tggcatagtc ggggtgattt
    11461 tcatcgcaac ctcgtctcgg taattgggtg ggttactccg gtaaagcggt taacaggcgg
    11521 caaaacttta atcgcctgcg caaattactc cgcgcttacc gtcgtcacca cgccgctttc
    11581 ggccaccatt gcgctgatga cccgctggct gctggcgtta cgcactttca ccatctcgcc
    11641 tttgcggcca tttttcagcg ccacgccggg catcgaggcg gtgatccccg cgctctggta
    11701 aacaatcatc accggttgat cgcgcccgac cagcaccgga gacacgagtt cttctttggt
    11761 gattggcctg ccgggttgca gggcgtgttt actgctgaaa ccaatcgcgt catcgagaac
    11821 catcaacaca tcgcggcgct gggcgctgac gttgtagggc ttaagctcca catcattcgc
    11881 ggtgagcggc atatcgcggg caaccagtga cttcgtcatt accaccggca ccagtacatc
    11941 cgggcgcacc gccacgttca tctgccagct ttgcgggcaa ctaacggtga agttcatccg
    12001 gttgagggcg atttccgcag gcgaagtcag cgtgacgccg ggtgttttag tacagggcgc
    12061 ggcggtcgca atctgcgagg gaatgtagat cttaaaggtg tagcgatagt catgccactg
    12121 tcgctgctgc gccagcgatt caattttttg gctcgcggca ttcagcacct gggcgttgac
    12181 ttgttcccgc gcgctgtgtt gcacgggatg gacggcggcg tagcccggta aggccagcgc
    12241 caggaggaag taaaacttcc gcctgcaaaa ttgaaaactc ataaaccctt tctcatcagt
    12301 gacttaaaaa atggcatggc gattgcttag cgtatgcgga ttgaagcagg agtgatgcag
    12361 gaaatgggga tcagttttca gcaggcattg ggagtacatc cgcaggcagt gaagttacgt
    12421 cttgagagga ccgagctact gactgcgaat ctggcgaacg tcgatacgcc aaatttcaag
    12481 gctaaagata ttgattttgc cagggagatg caacgggcaa ataacgcggc ggtggatgtc
    12541 cagtaccgcg tgccgatgca gccgtcggaa gatggcaaca ccgtggaact gaacagcgaa
    12601 caggcgcggt tttcacaaaa tagtatggat tatcaaagca gtttgacctt tctgaatctg
    12661 caaatcagcg gcatcagaga ggccattgaa gggaaataag catgtcattt actgatatct
    12721 atcagatttc cggctcggcg atgacggcgc aaacgctacg tctgaatacc gttgccagta
    12781 acctggcgaa tgccaatgcg cccgccagca gcgaggcgca ggcgtataaa gcgcgcagcc
    12841 cggtgtttgc cgcggtgtat caccacagcc tgctggcggg tacacatcgt catgccattg
    12901 acagtgccag cgtgcaggta caggacgtgc tgcaaaccgg cggggcggtg aaacgctatg
    12961 aaccgcattc tccgctcgcc gacgccaacg gtgacgtctg gtatcccgac gtcaacgtgg
    13021 tggagcagat ggcggacatg atgtcggcct cgcgcgattt cgaaaccaat gtcgatgtgc
    13081 tcaacaacgt gaaaagtatg cagcaaagtt tgctgaaact gggagaagcc tgatgaatac
    13141 cctggcgctg aatgcgcaat cgccgcagcc gaccgccacg gcggaaagca ataatgtggc
    13201 ggccagcgac acgcaaagca gcagcgatag ctcgcctgtc gataccttcc tgacgctgtt
    13261 tgtcgccgag atccaaaacc aggacccgac cgacccgacc gacgctaccg aatacatcga
    13321 ccagctgtcg tcgatggcgc aggtggcgat gatggaagag atgggcgtgc aggccaacac
    13381 caacgcggtg ctgatgagca acattcaggt gatggcgctc ggcaacctgg tgggcgacga
    13441 cattatggtg cagaccaccg ccttgcaggt gagcgaccag accattaatg gtcgcgctac
    13501 gctggacgac gcctgtacca ccgccgatct ccactttacc gatgccgcag gcgatgacta
    13561 caccgtctcg ctgatcccgc aagggagttc gtccgtcggg ccaggccagg tggatttctc
    13621 ggtgaatccc gcggattacg gtatcccgcc gggcgattac gacgtctcgg tggtcaccaa
    13681 caccggggaa gaagaggtgc cgattgaagt ctccggcgaa gtggaagatg tgcgcatccc
    13741 gctcgacggc agctctccgg tgctcaacgt tgcaggcgtg ggcgaagtgc cgttcaccat
    13801 gattagccag tttggcgtac ccgatgccga cagtgatgtg gcctgatttt gtaggccgga
    13861 taaggcgttc acgccgcatc cggcaaaaca acgggcacgc tgtcaaaatt tgaattaaca
    13921 ggaagacaca atgagttatg aaattgccgc gacggggctg aatgccgtta acgaacagct
    13981 cgacgggatc agtaacaaca tcgccaacgc cggaacggtg ggctataagt cgatgactac
    14041 ccagttttca gctatgtatg ccggaagcca ggcgatgggt gtcagcgtgg cgggcacggc
    14101 gcaaagtatt tcgcgcggcg gttcgctggt ctccaccggc aacgcgctgg atctggcgat
    14161 taacgacgac ggcttttttg ttacctgcga cagcgcgggc aacatttcct acacccgcgc
    14221 cggatcgttt gaaaccgaca aaaacggcta tattgtcaac gcctcgggcg cttatttgca
    14281 gggctatccg gtcgatgaca ctggcactct gcaaaccggt acggtcaccg atatccagat
    14341 caaaaccggc aatatcccgg cgcaggccag cagcagcctg acctttaccg ctaacttcaa
    14401 cgccagcgat gaagctatcg atcgcaccac cgttccgttc gacgcgacta acagcagttc
    14461 gtacaccgac agctacacca ccacggtgta cgactcatta ggcaacgaac attcggtatg
    14521 ccagtacttc accaaaacca gcgataacac ctgggaagtg cagtacacct tcgacggtca
    14581 gcagcagacc ggcgttccgg cgacgacgtt aaccttcgac ccgaacaccg ggaagctgac
    14641 ctctccaacc acgccgcaga ccattgaatt tcaaaccgac gctgccgcac ccatcgactt
    14701 aaccgttgat tactccacct gtacgcaata cggctctgaa ttttcggtca ccaccaacgc
    14761 cgccaacggc tacgcctccg caacgcaaaa cggtgtacag gttgatgacg atggcaaagt
    14821 ttacgccacc tacagcaacg gcgagcgtat gttgcagggc caggtggtgc tggcgacctt
    14881 cccgaatgaa aactgcctgg aggcggtgag cggcactgcg tgggtacaaa ccggcgaatc
    14941 cggcaccccg ctgattggcg ttcccggctc cggcgcctgc ggcacgctca cgtccggcgt
    15001 gctcgaaagc tctaacgtcg atatcaccag cgaactggtt aacctgatga ccgcccagcg
    15061 taactatcag gccaacacca aagttatctc caccagcacg cagctcgatg acgcgctgtt
    15121 ccaggcaatg taatggatcg cttaatttat accgccctga gcggggcttc tcagaccctg
    15181 tacgagcagc aaattagcgc caacaacctg gcgaacgtca acaccaacgg ttttcgcgcc
    15241 gatatggcga tggcgaccaa cgacaaggtg aaaggcggtg gcttcgacac gcgctatatg
    15301 gcgcaggaag gggccagcgg cgtgaacgac agcacgggcg tggcggagaa aaccgaacgc
    15361 ccgctggatg tggcgattca gggggcaggt tacattgcgg tacaggataa aaacggcaac
    15421 gaagtttata cccgcaacgg caacattcag caggacgacc aggggcaact gaccatcgac
    15481 ggcaatctgg tgctgggaga taacgggccg atcattctgc cgccgaatgc cattgcttcg
    15541 tttggcagcg acggcacgct gtcagtgacg cccgatgacg gcgacgtgac cgcgacgatg
    15601 gatatcgatc gcctgaagct ggtggatatc ccggtcgcta acctggagaa aaatgctgaa
    15661 gggatgctgg tgactgccga cggcgttccg gcccagcgcg atgaaaacat caaagtcagc
    15721 ggcggttttc tcgagggcag caacgtctcg gcggtaagcg aaatgatgtc gtccatcgcc
    15781 atgaaccgcc agttcgaagc gcagataaaa atgatgaaaa ccgccgaaga tatcagcgac
    15841 gcgggcaacc gcttattgcg cggctcgtaa ccgcacatcg taggccggat aaggcgttta
    15901 cgccgcatcc ggcaactatg cggcggtgcc tgatgcgacg ctttgcgtct tatcaggcct
    15961 acaagcctgt gcccaaaccg taggccggat aaggcgttca cgccgcatcc ggcaaccgca
    16021 gcccaaaaat aatacggaat ttaccaccat gaacgcagca ttatggatca gcaaaaccgg
    16081 cctctcggcg caggatgctg aaatgagcgc gattgccaac aacatcgcca acgttaacac
    16141 cactggcttc aagcgcgacc gggtgatgtt tcaggatctg ttttaccaga cccaggaagc
    16201 gccgggagcg atgctcgacc agaacaacat catgccgacc gggctgcaat tcgggagcgg
    16261 cgtgcgcatt gtcggcaccc agaaaacctt taccgaaggc aacgttgaaa ccaccgacaa
    16321 cgcgatgaat gtcgccatta tggggcaggg gtttttacag gtgcaaaagg ctaatggcga
    16381 tattgcctat acccgcgacg gcaatttgca ggtgaacgcc gatggcgtac tgaccaactc
    16441 acagggcttg ccattgcagc cggaaatcga cgttccggcg ggggcgacga atgttgcgtt
    16501 tggtgaggac ggcacggtga cggcgattct tcctggcgac agcgacgtga ctgagttagg
    16561 gcaactgaca ctggttaact tcgccaaccc ggcggggctt tccgctgagg gtgacaacct
    16621 ctatctggaa accgccgcca gcggtcagcc gaccgaaggc gtaccgggtg aagatgggct
    16681 tggcactttg caggacaacg cgctggaagg ctcgaatgtg gatatcgtca acgaaatggt
    16741 ggcgatgatt acggtgcaac gtgcctatga gatgaacgcc aaaatggtgt cggcagcgga
    16801 cgatatgttg cagtacatca gccagacgct gtaaggcgaa atgtcggatg cgacgctgac
    16861 gcgtcttatc cgacctacaa aagcgcataa ctcactgtat ttaaaacagg cagaacgtga
    16921 aaaattatct ctggctggtg gcactgcttc ccctgcttag cgggtgtgaa tcgcaggcca
    16981 ttctggtcaa aaaagacgac gcatactttg cgccaccgaa aaccgaagcg ccgcctccgg
    17041 cagacgggcg tgccgggggc gtgtttgaaa ctggctacaa ctggtcgctg accgccgacc
    17101 ggcgggctta ccgcgtgggc gatatcctga cggtgatcct cgaagaatcc acccagtcga
    17161 gcaaacaggc gaaaaccaac ttcggcaaaa gcaacaccgt cgatatcggc gcgccgacta
    17221 tttttggaca cactaaagat aagctttccg gctccatcga cgccaaccgc gactttgacg
    17281 gcagcgccac ttcccagcag cagaacagcc tgcgcgggga gagcactgtt tccgtccatg
    17341 cggtgcagcc caacggcatc ctggaaattc gcggcgagaa atggctgacc ctcaaccagg
    17401 gcgatgagta catccgttta agcggccttg tgcgcgccga cgacatccag aacgacaact
    17461 cggtttcctc ccagcggatc gccgacgccc gtatctccta tgccggacgc ggcgcgttaa
    17521 gcgacgccaa cgccgcaggc tggctgacgc ggctgtttaa ccatcctctg ttcccgattt
    17581 aatgccggag agtgcaatgc aaaactggat aaaggccgca gcgatcgctg taagtctgat
    17641 gctgcccggc gtggcgctgg cgcaatcgct ggagtcgttg gtcaacgtac aaggtgtgcg
    17701 tgaaaaccaa ctggtcggct acagcctggt ggtcggcctc gacggcaccg gtgataaaaa
    17761 ccaggtcaaa ttcaccaacc agaccatcac caatatgctg cgccagttcg gcgtgcagct
    17821 gccgaataaa atcgacccga aggtgaaaaa cgtcgccgcg gtggcggtga gtgccacgtt
    17881 gccgccaatg tactcgcgcg ggcagaccat tgatgtcacc gtttcgtcga ttggcgatgc
    17941 gaaaagtatt cgtggcggca ccttgctgtt gacccagttg cacggcgcag atggcgaagt
    18001 ttatgccctg gcgcagggca gcgtggtagt cggcgggatg aacgccaccg gggccagcgg
    18061 ttcgagcgta acggttaata cgccaaccgc tgggttaatt cccaacggcg cgacggtgga
    18121 gcgcgagatc cccagcgatt ttcagatggg cgacaccatc acgcttaatc tgaaacgtcc
    18181 gtcgtttaaa gacgctaaca acattgcggc ggcgatcaac gcctcgtttg gcggcattgc
    18241 caccgcgcag agctccacca acgtcaccgt gcgcgcaccc accagccccg gcgcgcgggt
    18301 ggcgtttatg tcgcaactgg atgatgtgca ggtgcaggcg gagaaaatcc gcgctcgcgt
    18361 ggtgtttaac tcgcgcaccg gcacggtggt gatgggcgat ggcgttgtgc tgcacgcggc
    18421 ggcggtgtcg cacggtagtt tgaccgtctc gattaacgaa accagcaacg tcagccagcc
    18481 gaatgccttt gcgggcgggc gcacggcggt aacgccgcag agcaatattg cggtgaatca
    18541 tgcccgtcct ggcgtggtga gtttgccgga gtcgagcagc ctgaaaacgc tggtcaatgc
    18601 gttgaacagt ttaggcgcga cgccggatga cattatgtcg attttgcagg cgctgcatga
    18661 agcgggtgcg ctggatgccg atctggaggt gatctgatga aattgaacgc cagcggcggg
    18721 attgacggca gcgacgcgtt aatggggccg aaggttcagg ccaatgatat taaacaggcc
    18781 gcggaacagt ttgaggcgat ttttttacgc aacatgctga aagagatgcg gaaaaccaac
    18841 gagttgtttg attcaaaaga taacccgttt aacagcgatt cggtgcgcat gatgcagggg
    18901 ttttatgacg atgagttgtg taacaccctg gcgcagcagc acgggattgg cattgcggcg
    18961 atgattgtga agcagttgtc gccgaagcat aaatgaggct ccgacgttcg gtgcacgatg
    19021 cctgatgcga cgctg
//
LOCUS       DAPPNJ010000084        18828 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000084 DAPPNJ010000000
VERSION     DAPPNJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.084"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..612)
                     /gene="rsmG"
                     /locus_tag="RPM73_002392"
     CDS             complement(<1..612)
                     /gene="rsmG"
                     /locus_tag="RPM73_002392"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDX6520742.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVCPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKA"
     gene            complement(676..2565)
                     /gene="mnmG"
                     /locus_tag="RPM73_002393"
     CDS             complement(676..2565)
                     /gene="mnmG"
                     /locus_tag="RPM73_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDX6520743.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELSLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2944..3387)
                     /gene="mioC"
                     /locus_tag="RPM73_002394"
     CDS             complement(2944..3387)
                     /gene="mioC"
                     /locus_tag="RPM73_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDX6520744.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3477..3935)
                     /gene="asnC"
                     /locus_tag="RPM73_002395"
     CDS             complement(3477..3935)
                     /gene="asnC"
                     /locus_tag="RPM73_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDX6520745.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4087..5079
                     /gene="asnA"
                     /locus_tag="RPM73_002396"
     CDS             4087..5079
                     /gene="asnA"
                     /locus_tag="RPM73_002396"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDX6520746.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5084..6535)
                     /gene="viaA"
                     /locus_tag="RPM73_002397"
     CDS             complement(5084..6535)
                     /gene="viaA"
                     /locus_tag="RPM73_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDX6520747.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6529..8025)
                     /gene="ravA"
                     /locus_tag="RPM73_002398"
     CDS             complement(6529..8025)
                     /gene="ravA"
                     /locus_tag="RPM73_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDX6520748.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8248..10116
                     /gene="kup"
                     /locus_tag="RPM73_002399"
     CDS             8248..10116
                     /gene="kup"
                     /locus_tag="RPM73_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDX6520749.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10283..10702
                     /gene="rbsD"
                     /locus_tag="RPM73_002400"
     CDS             10283..10702
                     /gene="rbsD"
                     /locus_tag="RPM73_002400"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDX6520750.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10710..12215
                     /gene="rbsA"
                     /locus_tag="RPM73_002401"
     CDS             10710..12215
                     /gene="rbsA"
                     /locus_tag="RPM73_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDX6520751.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12220..13185
                     /gene="rbsC"
                     /locus_tag="RPM73_002402"
     CDS             12220..13185
                     /gene="rbsC"
                     /locus_tag="RPM73_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDX6520752.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13210..14100
                     /gene="rbsB"
                     /locus_tag="RPM73_002403"
     CDS             13210..14100
                     /gene="rbsB"
                     /locus_tag="RPM73_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDX6520753.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14226..15155
                     /gene="rbsK"
                     /locus_tag="RPM73_002404"
     CDS             14226..15155
                     /gene="rbsK"
                     /locus_tag="RPM73_002404"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDX6520754.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKEAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15159..16151
                     /gene="rbsR"
                     /locus_tag="RPM73_002405"
     CDS             15159..16151
                     /gene="rbsR"
                     /locus_tag="RPM73_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDX6520755.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16117..17544)
                     /gene="mdtD"
                     /locus_tag="RPM73_002406"
     CDS             complement(16117..17544)
                     /gene="mdtD"
                     /locus_tag="RPM73_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDX6520756.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17567..18259)
                     /locus_tag="RPM73_002407"
     CDS             complement(17567..18259)
                     /locus_tag="RPM73_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDX6520757.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            18741..>18828
                     /locus_tag="RPM73_002408"
     rRNA            18741..>18828
                     /locus_tag="RPM73_002408"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4561 a         4657 c         4913 g         4697 t
ORIGIN      
        1 tgctttaatc accaccagat gacgttcgcc atccagggct ggaacctgaa gtttaaccac
       61 tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt
      121 cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt
      181 cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc
      241 taccctgctc tgtactggtt caatattctc cagtttaagc tcatgttgca cctgacgaag
      301 gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacacacgat
      361 agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg
      421 cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc
      481 gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat
      541 aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca gtaaggagag
      601 tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca
      661 ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag
      721 cagaatggag atggccgcag gcgtgacgcc agaaatacgc gaagcttggc cgatagaggc
      781 tggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta
      841 atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg
      901 ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc
      961 gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg
     1021 acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt
     1081 cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc
     1141 acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc
     1201 cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgta gcatcagacg
     1261 atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc
     1321 atcaactagt acgccgagat acgcctgaga acgtgccgga gcccaacctt ctttgtcagc
     1381 agacagacgg gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc
     1441 ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt
     1501 cggtttcagg tcgcgaggat cgaagaagtc atactcaatg gcataacccg gacgcacgat
     1561 cttcgcgttt tccatcccct gcatagagcg gacgatttgc atctgcacat cgaacggcag
     1621 gctggtggag ataccgttcg gataaatttc gttagaggtc agtccttccg gttcaaggaa
     1681 gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg acgggcagta
     1741 gcgtgggccg acaccttcga tcacccctgc atacattggg ctacgatcga ggttactgcg
     1801 gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg
     1861 ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc
     1921 cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg
     1981 accaacgcgc agcgacagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc
     2041 agcacggcca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga ggaacgtccc
     2101 aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac
     2161 cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg
     2221 ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg
     2281 ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga tacccgcttg
     2341 atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt
     2401 cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt
     2461 caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg
     2521 acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca
     2581 taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct
     2641 tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc
     2701 tattgtgatc tcttattagg atcgcactgc cctgtggata acaaggatcc ggcttttaag
     2761 atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc
     2821 tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg ttccttggat
     2881 aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta
     2941 tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat
     3001 gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt
     3061 gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat
     3121 accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata
     3181 gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taatcagcca
     3241 gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc
     3301 cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct
     3361 gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt
     3421 acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag
     3481 ggcttgatag tacgcatgat cgggttttgc aggacgatca atgtctcggt ggactgaatt
     3541 tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac
     3601 attactttta taaagatgct gtagtggccg gttgtgtaat aggcttcagt gacttcatca
     3661 aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa tataatgccg
     3721 ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta
     3781 atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca
     3841 aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg
     3901 ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc agattgatga
     3961 ttcattctat tttagccttc ttttttaatg aatcaaaagt gtgttaggct ttttattgaa
     4021 tgattattgc atgtgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta
     4081 taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac
     4141 ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc
     4201 cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa
     4261 gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt
     4321 cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc
     4381 cttcgccccg atgaagagcg tctttctccg ttgcactcgg tctatgttga ccagtgggac
     4441 tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag
     4501 gcgatctggg cgggaattaa agcaaccgaa gctgcagtta gcgaagagtt tggtttggca
     4561 ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg
     4621 gatctcgatg ccaaagggcg tgagcgggcg attgcgaaag atcttggcgc ggtattcctc
     4681 gtcgggattg gcggcaagct gagtgatggt catcgtcacg acgtgcgcgc accggattat
     4741 gatgactgga gcaccccgtc agagctgggc catgcgggtc tgaacggcga tattctggta
     4801 tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc
     4861 gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgga gctggagtgg
     4921 catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct
     4981 cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg
     5041 ccagctgctg ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc
     5101 tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga
     5161 tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct
     5221 gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa
     5281 tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa
     5341 aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt
     5401 cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat
     5461 agcagcgccg gttttctgcg agagcaatgc gcatcaaggc caggcagaac gctttcgcac
     5521 actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata aacggcccgc
     5581 gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt tcacgccacg
     5641 actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat
     5701 actccagttc cgttatccct agtgtcgcca gttctggcgg cagaagacgt aaaatatcat
     5761 cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc accatggtgc
     5821 ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccga gaacgcccca
     5881 gctgctctgc caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa
     5941 tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga cgaccagctg
     6001 cagtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa
     6061 cttcactcaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac gtcgttgctt
     6121 gcacgatcag acttaaacgc caacgctgga gaaaaagcgt gtgtaacgct gaagtgatcg
     6181 tgctgttagc atcaaccaac tgtcgggctt gttctgccca tggagaattc agacgatgca
     6241 gtaagtccag gatctgtggt agctgcacaa taaactgtgg cgtggagagg agctggcttt
     6301 gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgac
     6361 tgcgcagcgc ttcacgccag cggggaacat catcagtgat tgccgccttc aggcgtggga
     6421 atttttcaaa gaagaccgcc agctgcggtg aggccagcag tgcgatgatc atctcttcga
     6481 tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg
     6541 tgcctggcga atctgtgcac cgacatcctg taggctggct tcaatgcgac ctaaccagtc
     6601 gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct tgcgccagtc
     6661 gctttcaagc tcttccagtt gttgttttat ttcaccaggc agaccttcag ccgatgaacc
     6721 gggaagtgcc agcgtactgc cttgtaaact tacatcgcga acgacaagat gttgggcgct
     6781 atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttac cgcgaatttc
     6841 accacctttg ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc
     6901 catatcatgc agttttaacg gtttttgcag cagtagagtc agagtggaag cagtaacgtt
     6961 aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa
     7021 ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgccaag
     7081 gcgagtcaac atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg
     7141 ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc
     7201 taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata agcggatcgc
     7261 ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg
     7321 cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg
     7381 ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc
     7441 atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca
     7501 cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc
     7561 gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac cgttgcggaa
     7621 ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat
     7681 ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg
     7741 cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa
     7801 gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc
     7861 gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc
     7921 cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact
     7981 gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc
     8041 tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata
     8101 gtctcaaagg gggggtattt ttctttgagc caggttaatg tggccgcatt taggagtacg
     8161 attttgccgt taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca
     8221 tttcatattt caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg
     8281 attaccctcg cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca
     8341 cttcgtgaat gtttgtccgg ccagtttggt tttggcgttg aacgcgatgc cgtgtttggc
     8401 tttttatcgc tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc
     8461 gtgatgcggg cagataacgc cggtgagggg gggatcctga cgttgatgtc gcttgccggg
     8521 cgtaatacgt cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagc
     8581 tttttctatg gtgaagtcgt cataacaccc gctatttcgg tgatgtcagc cattgaaggt
     8641 ctggaaatcg tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc
     8701 actttattat ttatgattca aaaacatggc acggctatgg tcggtaagct gtttgcgccg
     8761 atcatgctga cctggttttt gattctggca ggactggggt tacgtagcat tattgctaac
     8821 ccggaagtgc tgcatgcgct gaatccaatg tgggcggtgc atttcttcct tgaatacaaa
     8881 acggtttctt ttattgcatt aggggcagtg gtgttgtcga ttacgggggt cgaggcgctg
     8941 tatgctgata tggggcactt tggtaagttc cctattcgcc tggcgtggtt caccgtcgta
     9001 ttaccttcct tgacccttaa ttacttcggt cagggagcgc tgttgttaaa gaacccggaa
     9061 gcgattaaga acccgttctt cctgttggca ccggactggg cgctgatccc gctgctgatc
     9121 atcgccgcac tggcgacggt aattgcctcg caggcggtta tctctggcgt cttctcattg
     9181 acgcgtcagg cggtacgtct gggatatttg tcgccgatgc gcattattca cacctccgaa
     9241 atggagtcag ggcaaatcta tattccgttt gtgaactgga tgctctatgt cgcggtcgtg
     9301 attgtgattg tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg
     9361 accggaacca tggtgctgac gtctattctc tcgactaccg tggcacgtca gaactggcac
     9421 tggaataagt attttgttgc gctgatcctg attgctttcc tttgtgtcga tattccattg
     9481 ttcaccgcta acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtact
     9541 gtgatgttta tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg
     9601 catgaacatg gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcctgtt
     9661 cgcgtgcccg ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg
     9721 atgcataacc ttaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgt
     9781 accgaagacg cgccatatgt ccataatgtc cgtcgggtac agattgaaca actgtcgccc
     9841 actttctggc gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt
     9901 ttccaccgct gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg
     9961 tcgcatgagt cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg
    10021 tacttgctgc tgcaacgtaa tgcgctgcgt gcaccagatc aatttgaaat tccgccaaac
    10081 agggttatcg aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttctccata
    10141 agggggcgtt tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat
    10201 ttccgtaacg tcacgatggt tttcccaact cagtcaggat taaactgcgg gtcagcgaaa
    10261 cgtttcgctg atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc
    10321 ggtgatctcc cgtctgggac ataccgatac gctggtggtg tgtgatgctg gtttacccat
    10381 ccccaaaagt acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca
    10441 ggtgctgggc gtcgtcacaa atgaaatgca ggtcgaggct gccattatcg cggaagagat
    10501 caaacaacat aatccgcaac tccacgaaac gttgctcact caccttgagc agctgcaaaa
    10561 acaccaggga aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac
    10621 cgcagaaagt caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct
    10681 ctgtgctggc gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg
    10741 ataaagcctt cccgggcgta aaagccctct cgggcgcagc gttaaatgtc tatccgggcc
    10801 gcgtgatggc gctggtgggc gaaaacggcg cgggtaaatc caccatgatg aaagtgctta
    10861 ctggtatcta tgctcgcgat gccggcacgc ttttatggct ggggaaagaa acgacattta
    10921 ccgggccgaa atcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga
    10981 tcccgcagtt gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg
    11041 gcaaaattga ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc
    11101 tgcgctttaa aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg
    11161 aaatcgccaa agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg
    11221 cgctgaccga taccgaaacc gaatccctgt tccgcgtcat acgcgagctg aaatcgcaag
    11281 gccgcggtat tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg
    11341 ttaccgtttt tcgtgacggg caatttattg ctgagcgcga agtggcatca ctgaccgaag
    11401 attcgctgat tgagatgatg gtgggccgca agctggaaga tcaatatcca cacctggaca
    11461 aagcgccggg agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg
    11521 tctcttttac tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc
    11581 gtaccgaact gatgaaagtg ctctacggcg cactaccgcg caccagcggt tacgtcaccc
    11641 tggatgggca tgaagtcgtt acccgttcac cgcaggatgg cctggcaaac ggcattgtgt
    11701 atatctccga agaccgtaaa cgtgacggtt tagtgttggg catgtcagta aaagagaaca
    11761 tgtcgctgac agcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg
    11821 aacagcaggc tgtgagtgat ttcattcgtc tgtttaatgt gaaaacaccg tcgatggaac
    11881 aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga
    11941 tgacacgccc caaagtgttg atccttgatg aacctacccg tggcgtagat gtcggcgcga
    12001 aaaaagagat ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg
    12061 tgtcatcgga gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag
    12121 ggcatctcag cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg
    12181 ctgtgggcaa gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct
    12241 ggtcgccgtt atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg
    12301 gtgctgatcg cgattgtctc gacgttaagc ccgaactttt tcaccatcaa taacttattc
    12361 aatattcttc agcaaacctc ggtgaacgcc attatggcgg tcgggatgac gctggtgatc
    12421 ctgacgtcgg gtatcgactt gtcggtaggt tctctgttgg cgctgaccgg cgcagttgct
    12481 gcatctatcg tcggcattga agtcaatgcg ctggtggctg tcgctgccgc tctcgcgtta
    12541 ggtgccgcaa ttggtgcggt aaccggggtg attgtagcga aaggtcgcgt ccaggcgttt
    12601 atcgctacgc tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt
    12661 agcccagtga ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt
    12721 cgtccgctgg gcgtaccgac gccagtctgg atcatgggga ttgtcttcct cgcggcctgg
    12781 tacatgctgc atcacacgcg tctggggcgt tacatctacg cgctgggcgg caacgaagcg
    12841 gcaacgcgtc tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt
    12901 ggtctgctgg catcgctggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc
    12961 acggcgggga ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt
    13021 ctggcgggcg gaaaaggtcg cattgttggg acgttgatcg gcgcattaat tcttggcttc
    13081 cttaataatg gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg
    13141 gtggtgattt tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac
    13201 atcttgaata tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc
    13261 accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc cacgcttaac
    13321 aacccgttct ttgtatcgct gaaagatggc gcgcagaaag aggcggataa gcttggctat
    13381 aacctggtgg tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtgcaggac
    13441 ttaaccgttc gcggcaccaa aattctgctg attaacccga ccgactccga cgcagtaggt
    13501 aatgctgtga agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca
    13561 acgaaaggtg aagtggtgag ccacattgct tctgataacg tactgggtgg caaaatcgcc
    13621 ggtgattaca tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct gcaaggcatt
    13681 gctggtacat ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt tgctgctcac
    13741 aagtttaatg ttcttgccag ccagccagca gattttgatc gcactaaagg tttgaacgta
    13801 atgcagaacc tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa
    13861 atggcgctgg gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc
    13921 ggatttgacg gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact
    13981 atcgctcagc tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg
    14041 aaaggcgaga aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag
    14101 ttttaatcag gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt
    14161 aatataaaga aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc
    14221 cgaatatgca aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc
    14281 ttaatcttca atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg
    14341 catttggcgg caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg
    14401 cgtttattgc ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca
    14461 ctgataacat tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc
    14521 tgatttttgt taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg
    14581 ccctttcccc ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat
    14641 taatgcagct ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa
    14701 ataagactat cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg
    14761 cgctggtgga cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg
    14821 ttgaaaatga tgaagatgca gcgaaggagg cgcaggtact gcatgaaaaa ggtatccgta
    14881 ctgtactgat tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc
    14941 gcgttcctgg attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg
    15001 gcgcgttaat cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc
    15061 atgctgccgc tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg
    15121 aagagatcga cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg
    15181 cccgcctggc gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg
    15241 tcagtgaagc gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat
    15301 cagccctggc gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg
    15361 ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac
    15421 gcggttatag tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc
    15481 tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc
    15541 aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg
    15601 ctccgttcga tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag
    15661 caacgcaata tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg
    15721 ataaaactcc ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc
    15781 tcaacattcc tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg
    15841 ccatgcgcca actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg
    15901 ctatggctgt tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata
    15961 tcgcggtgat tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca
    16021 ctatccacca accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga
    16081 taacccagcc gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg
    16141 gttcggctta gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag
    16201 catgaacatt gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa
    16261 ttgttcgaca gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac
    16321 agcaacgcct aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct
    16381 ggcgttgtca tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga
    16441 catagccatc cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga
    16501 ttgcaaagag aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg
    16561 gcgatagccc agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa
    16621 cgctgtcggt gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa
    16681 catcaatggc ataaggaacg gtacacaacc ggtccccaga cgggttgcaa tattgcctac
    16741 gataccgatc gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt
    16801 gtgtcgcgca tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa
    16861 ggcaatccag ctggcgacaa tcctttcccc gaatagctct attccgcttg agaagagaac
    16921 aaggctgagg ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa
    16981 attgggcata tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa
    17041 aaatatccag tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat
    17101 tggccccacc agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg
    17161 ataagcgcgc agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg
    17221 aataacccgg aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc
    17281 caatgtgaac agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag
    17341 ccatccgctt accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg
    17401 catcgcgaga ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat
    17461 agtggcatca agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat
    17521 actgcgcttc tttttatcgc tcattcaatg tcctgttatc gggttgtcac ttatcaggtg
    17581 agcgtagcag cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat
    17641 cgacaatcgc ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt
    17701 aagtgtgata aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca
    17761 tttcatagat atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt
    17821 tctcttttaa tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg
    17881 tgccaaccgt tgcagccagt aggcaagctt gcggctccag acagatacgc ataaccagaa
    17941 agtgatcgat gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat
    18001 caagaaaatt ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa
    18061 ccatcccttt tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa
    18121 attgctcgcc cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta
    18181 agatgcgttg cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact
    18241 gttgtgcgct taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc
    18301 accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac
    18361 gggggctttt gctgaaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    18421 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
    18481 ggtcagcggg gttctcctga gaattccgac agagaaagca aaaataaatg cttgactctg
    18541 tagcgggaaa gcgtattatg cacaccccgc gctgctgaga aaaagcgaag cggcactgct
    18601 ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc
    18661 aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat
    18721 tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc
    18781 ggcaggccta acacatgcaa gtcgaacggt aacaggaagc agcttgct
//
LOCUS       DAPPNJ010000085        18330 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000085 DAPPNJ010000000
VERSION     DAPPNJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.085"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            14..88
                     /gene="tnaC"
                     /locus_tag="RPM73_002409"
     CDS             14..88
                     /gene="tnaC"
                     /locus_tag="RPM73_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDX6520758.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            309..1724
                     /gene="tnaA"
                     /locus_tag="RPM73_002410"
     CDS             309..1724
                     /gene="tnaA"
                     /locus_tag="RPM73_002410"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDX6520759.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1815..3062
                     /gene="tnaB"
                     /locus_tag="RPM73_002411"
     CDS             1815..3062
                     /gene="tnaB"
                     /locus_tag="RPM73_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDX6520760.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3194..4369
                     /gene="mdtL"
                     /locus_tag="RPM73_002412"
     CDS             3194..4369
                     /gene="mdtL"
                     /locus_tag="RPM73_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDX6520761.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4344..5303
                     /gene="yidZ"
                     /locus_tag="RPM73_002413"
     CDS             4344..5303
                     /gene="yidZ"
                     /locus_tag="RPM73_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDX6520762.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5460..6209
                     /locus_tag="RPM73_002414"
     CDS             5460..6209
                     /locus_tag="RPM73_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDX6520763.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6231..6797
                     /gene="yieF"
                     /locus_tag="RPM73_002415"
                     /gene_synonym="chrR"
     CDS             6231..6797
                     /gene="yieF"
                     /locus_tag="RPM73_002415"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDX6520764.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6851..8188)
                     /gene="adeP"
                     /locus_tag="RPM73_002416"
     CDS             complement(6851..8188)
                     /gene="adeP"
                     /locus_tag="RPM73_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDX6520765.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8355..9020
                     /gene="yieH"
                     /locus_tag="RPM73_002417"
     CDS             8355..9020
                     /gene="yieH"
                     /locus_tag="RPM73_002417"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDX6520766.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9087..9560
                     /locus_tag="RPM73_002418"
     CDS             9087..9560
                     /locus_tag="RPM73_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520767.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLILYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9609..10196
                     /locus_tag="RPM73_002419"
     CDS             9609..10196
                     /locus_tag="RPM73_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDX6520768.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10258..10980)
                     /locus_tag="RPM73_002420"
     CDS             complement(10258..10980)
                     /locus_tag="RPM73_002420"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDX6520769.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10995..12164)
                     /locus_tag="RPM73_002421"
     CDS             complement(10995..12164)
                     /locus_tag="RPM73_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDX6520770.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12191..13807)
                     /gene="bglH"
                     /locus_tag="RPM73_002422"
     CDS             complement(12191..13807)
                     /gene="bglH"
                     /locus_tag="RPM73_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDX6520771.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDVALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWIFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13893..15287)
                     /gene="bglB"
                     /locus_tag="RPM73_002423"
     CDS             complement(13893..15287)
                     /gene="bglB"
                     /locus_tag="RPM73_002423"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDX6520772.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15306..17183)
                     /gene="bglF"
                     /locus_tag="RPM73_002424"
     CDS             complement(15306..17183)
                     /gene="bglF"
                     /locus_tag="RPM73_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDX6520773.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17317..18153)
                     /gene="bglG"
                     /locus_tag="RPM73_002425"
     CDS             complement(17317..18153)
                     /gene="bglG"
                     /locus_tag="RPM73_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDX6520774.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4631 a         4569 c         4322 g         4808 t
ORIGIN      
        1 ggataagtgc attatgaata tcttacatat atgtgtgacc tcaaaatggt tcaatatcga
       61 caataagatt gtcgatcacc gcccttaatt tacccttctg tagccatcac cagagccaaa
      121 ccgattagat tcaatgtgat ctatttgttt gctatatctt aattttgcct tttgcaaagg
      181 ccatctctcg tttatttact agttttagta aatgatggtg cttgcatata tatatggcga
      241 attcatggat attgcaaatg taatattcac agggatcact gtaattaaaa taaatgaagg
      301 attatgtaat ggaaaacttt aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc
      361 cagtaaaacg taccactcgc gcttatcgtg aagaggcaat tattaaatcc ggtatgaacc
      421 cgttcttgct ggatagcgaa gatgtgttta tcgatttact gaccgacagc ggcaccgggg
      481 cggtgacgca gagcatgcag gctgcgatga tgcgcggcga cgaagcttac agcggcagcc
      541 gcagctacta tgcgttagct gagtcagtta aaaatatctt tggttatcaa tacactattc
      601 caactcacca gggccgtggc gcagagcaaa tctatattcc ggtactgatt aaaaaacgcg
      661 agcaggaaaa aggcctggat cgcagcaaaa tggtggcatt ctctaactat ttctttgata
      721 ccacccaggg ccatagccag attaacggct gtaccgtgcg taacgtctat atcaaagaag
      781 ccttcgatac tggcgtgcgt tacgacttta aaggcaactt tgacctcgaa ggattagagc
      841 gcggtattga agaagttggc ccgaataacg tgccgtatat cgttgcaacc atcaccagta
      901 actctgcagg cggtcagccg gtttcactgg caaacttaaa agcgatgtac agcatcgcga
      961 agaaatacga tattccggta gtaatggact ccgcgcgctt tgctgaaaac gcctatttca
     1021 tcaagcagcg tgaagcagaa tacaaagact ggaccatcga gcagatcacc cgcgaaacct
     1081 acaaatatgc cgatatgctg gcgatgtccg ccaagaaaga tgcgatggtg ccgatgggcg
     1141 gcttgctgtg catgaaagac gacagcttct ttgatgtgta caccgagtgc agaacccttt
     1201 gcgtggtgca ggaaggcttc ccgacatatg gcggcctgga aggcggcgcg atggagcgtc
     1261 tggcggtagg tctgtatgac ggcatgaatc tcgactggct ggcttatcgt atcgcgcagg
     1321 tacagtatct ggtcgatggt ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc
     1381 acgcggcatt cgttgatgcc ggtaaactgc taccgcatat cccggcagac cagttcccgg
     1441 cacaggcgct ggcctgcgag ctgtataaag tcgccggtat ccgtgcggta gaaattggct
     1501 ctttcctgtt aggccgcgat ccgaaaaccg gtaaacaact gccatgcccg gctgaactgc
     1561 tgcgtttaac cattccgcgt gcaacatata ctcaaacaca tatggacttc attattgaag
     1621 ccttcaaaca tgtgaaagag aacgcggcga atattaaagg gttaaccttt acctacgaac
     1681 cgaaagtatt gcgtcacttc accgcaaaac tgaaagaagt ttaattaata ctactgagtg
     1741 gctataagga tgttagccac tctcttaccc tacatcctca ataacaaaaa tagccttcct
     1801 ctaaaggtgg catcatgact gatcaagctg aaaaaaagca ctctgcattt tggggtgtta
     1861 tggttatagc aggtacagta attggtggag gtatgtttgc tttacctgtt gatcttgccg
     1921 gtgcctggtt tttctggggt gcctttatcc ttatcattgc ctggttttca atgcttcatt
     1981 ccgggttatt gttattagaa gcaaatttaa attatcctgt cggctccagt tttaacacca
     2041 tcaccaaaga tttaatcggt aacacctgga acattatcag cggtattacc gttgccttcg
     2101 ttctctatat cctcacttat gcctatatct ctgctaatgg tgcgatcatt agtgaaacga
     2161 tatcaatgaa tttgggctat cacgctaatc cgcgtattgt cgggatctgc acagccattt
     2221 tcgttgccag cgtattgtgg ataagttcgt tagccgccag tcgtattacc tcattgttcc
     2281 tcgggctgaa gattatctcc tttgtgatcg tgtttggttc tttcttcttc caggtcgatt
     2341 actccattct gcgcgatgcc accagcacca ctgcgggaac gtcttacttc ccgtatatct
     2401 ttatggcttt gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt cccagcctga
     2461 ttatttgcta tggcaaacgc aaagataagc taatcaaaag cgtggtcttc ggttcgctgc
     2521 tggcgctggt gatttatctc ttctggctct attgcacgat ggggaatatt ccgcgggaaa
     2581 gctttaaggc gataatctcc tcaggcggca acgttgattc gctggtcaaa tcgttcctcg
     2641 gcaccaaaca gcacgggatt atcgagtttt gcctgctggt gttctccaac ttagctgttg
     2701 ccagttcgtt ctttggtgtc acgctggggt tgttcgatta tctggcggat ctgtttaaga
     2761 ttgataactc ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg ccacccgcgt
     2821 tgttgtatct gatcttcccg aacggtttta tttacgggat cggcggtgcc gggctgtgcg
     2881 ccaccatctg ggcggtcatt attcccgcag tgcttgcaat caaagctcgc aagaagtttc
     2941 ccaatcagat gttcacggtc tggggcggca atcttattcc ggcgattgtc attctctttg
     3001 gtataaccgt gattttgtgc tggttcggca acgtctttaa cgtgttacct aaatttggct
     3061 aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat cacttatgtc
     3121 caaatggcaa ctcgcctgat tctccttcac cacgtatgct ttgcgtcacc ttactatcag
     3181 gacgctttag cccatgtccc gctttttaat ttgtagtttt gccctggttt tactttatcc
     3241 cgccgggatt gatatgtacc tcgttggttt accgcgcatc gccgccgatc tcaatgccag
     3301 cgaagcgcag ttgcatattg cgttctccgt ttatctggcg ggcatggcag ctgcgatgtt
     3361 atttgccggt aaagtggccg atcgttcagg gagaaagcct gtcgccatcc ccggcgcggc
     3421 gctatttatt atcgcttcgg tgttctgttc actggctgaa accagcacgt tatttcttgc
     3481 agggcgcttt ctacaggggt tgggcgcagg ctgttgttac gtggtggcgt tcgccatttt
     3541 gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcgttactca acggtattac
     3601 ctgcatcatt ccggtgttag cgccggtgct cggacacctg attatgctta aattcccttg
     3661 gcagagtctg ttctggacga tggcaatgat gggcatcgcg gtactgatgt tatctttgtt
     3721 tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac gagaaaatag
     3781 cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc tcagcgtttc
     3841 ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca tgggttttga
     3901 gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcatga ccgtttcatt
     3961 ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca cctcgcaggt
     4021 gttattcctt gcggcgggga tcacccttgc cgtttcacct tcccatgcgg tttctctgtt
     4081 tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga tgagtcaggc
     4141 gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta ttgcgcaggt
     4201 ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtatcggcg catggaatat
     4261 gctgatcggg attctgattg cctgtagcat agtgagcctg ttactgatta tgttcgtcgc
     4321 gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat ctcaatctgc
     4381 tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg aagcggatga
     4441 acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg tttgacgacc
     4501 cgctctttgt gaactcaccg ctggggctgt cgcccacacc gctaatggtc agcatggagc
     4561 aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg caccaccaga
     4621 caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg atcatgctta
     4681 atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa ctacgtaact
     4741 gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt ttttccggtc
     4801 gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc attgattatg
     4861 aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg gcactgcatg
     4921 aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc tgggaacaga
     4981 gcgacacctg ggcgctggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta
     5041 tgagcctgcc ggaatttgag cagtcactgt ttatggcagc gcaacccgac aatctgctac
     5101 tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg ccgttggttg
     5161 ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct tttaccctgc
     5221 tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa accattaaaa
     5281 acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt tcacaataaa
     5341 atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca
     5401 tcattccagt aaatgattac tcttatattc ataatggacc attaagcatg gagcgaaaaa
     5461 tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc
     5521 cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg
     5581 cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg
     5641 aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt
     5701 ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc
     5761 tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc
     5821 acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct aacgaagcgc
     5881 gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg
     5941 acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa
     6001 atcatgtaga agcgttatgc gacgcggagg acgtgctagt gtggtccgtg gcggtcacgc
     6061 ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg aaaagcctgc
     6121 cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct
     6181 ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt atgtctgaaa
     6241 aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg
     6301 cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg
     6361 ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg
     6421 aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata
     6481 actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc
     6541 aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg
     6601 cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg gtgatgaaca
     6661 agccggaatt tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga
     6721 ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt gagtttattc
     6781 agcgagttaa gatctaaata aaaaacccgc cagcaatcat gcatggcggg tttttaacgc
     6841 gctatcgatt ttagtgagcg tcgataaaga caatcttcag gataaacagc agcgcaacga
     6901 tgattacgca cgggcttaga tcacgcagac gtccggtacc gattttcatc acgcagtagg
     6961 agataaagcc cagcgcgata ccttcggtaa tcgagaagct gaacggcatc atcacggcgg
     7021 taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgtgcc agactggaag
     7081 tcatcagcac gccaacgtaa atcagcgcac cagctgcagc gtagcctggc accatccccg
     7141 ccagcggcga cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac
     7201 cggtacgacc gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag
     7261 taccgataaa cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc
     7321 gcgggaattt acccttctca tccgccaggc ctgctttatc ggtcacgcca atcagcgtac
     7381 cggaggagtc aaacaggttg accagcatga aagagaatat cacccctgcc agcccgaggt
     7441 taaacgaccc ggctaaatct acatgaccca caactgtcat tacgctcggc ggcgcagaaa
     7501 cgatgccatt gtagtgcaca tcacccagca tccagcccag cagcgtcgtc accacgatag
     7561 aaaccagcac cgctgcgtga atgttgcgcg aggccagaat agcaatgatg aagaagccga
     7621 ggatacccag aagtacgctg tgagaagtca gattaccgat gctcaccagc gtttccgggt
     7681 tagcgacaat cacacctgcg tttttcagcc ccatcatgcc aatgaacaga ccgataccgc
     7741 tggtaatacc cacacgcaga ctcaccggaa tgttggcaat catccagtag cgaacgcgga
     7801 aaatcgtcag taacagcaga cctatcgcgc cccagaagat tgcgcccatc ccgacctgcc
     7861 acggcaagcc catcgcctgt acaacgacaa aagcgaagaa cgcattcagg cccatagcgg
     7921 gtgccagtgc aactggcagg ttagcaaaca gtcccatcat aatactgccg aatgcagcga
     7981 tcagacaggt agtgacgaag acggcgctgg tatccatgcc agcaacgcca agaatttgcg
     8041 ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt aaaaccggcg atcacttcgg
     8101 tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg ttccagcatc ccctggccag
     8161 aagcctgggt tgtgtgttgt tgactcatta tctatttccg aacaaggagg gaaaatccgt
     8221 cgctatcgta taccaaaatg agacaatatg gacggctgaa gataactttt tttcattgta
     8281 tttgcttata cggcaacgat tgcgtcatgc gaattggcat gacgaaaacg ttaaactaac
     8341 gataaaggaa agacatgtcc cagatagaag cggtattttt cgactgcgac ggtacgctgg
     8401 tcgacagtga agtcatttgc tcccgcgcat atgtaacgat gtttcaggaa tttggtatta
     8461 cgctcgatcc tgaagaggta ttcaaacgtt tcaaaggtgt aaaactgtac gaaattatcg
     8521 atattgtttc ccttgaacat ggtgttacgt tagcgaaaac agaagctgaa cacgtttacc
     8581 gtgcagaagt cgctcggctg ttcgattcag aactggaagc catcgaaggg gctggagcgc
     8641 tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa cggcccaaat aacaaaatgc
     8701 agcattctat gggcaagctg aatatgttgc actacttccc ggataaactg ttcagcggct
     8761 acgatattca gcgctggaag ccagacccgg cgttaatgtt ccatgcggca aaagcgatga
     8821 atgtaaatgt agaaaactgc attctggttg atgactcagt tgccggtgcg caatctggta
     8881 tcgacgcagg tatggaagtg ttctacttct gcgccgaccc gcacaataag ccgatcgttc
     8941 acccgaaagt caccaccttt acccatcttt cgcagttacc ggaactgtgg aaagcgcgtg
     9001 gttgggatat tacggcatag ttcttcacac tcccttcact taccccgctt aaattggcgc
     9061 tcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg tcgggtaata catcacggac
     9121 tttattttgc ggttttagga ccgttaattg gtgttctgtt tcttatcctc tacatattct
     9181 tcgcaaaaga accgctggtt cttttgcgaa gaatacaggt acttccattg tttctcttat
     9241 tgtcgattac tacgggagtt attcctgcat tgttaaccgg cgtgatggtc gcctgtctgc
     9301 cggaaaagat cgggtcacag aaaaattatc gttgtctggc tggtggcata ggtggcgtcg
     9361 ttatcaccga gatctattgt gcagttattg tacatattaa gggcatggct tcctcggagt
     9421 tgtatgaaaa cattctttct ggtgacagtc tcgttgtccg catcattcct gcattgctgg
     9481 caggtgtggt gatgagcaga atcattaccc gtctgcccgg attggatatt tcatgtcctg
     9541 aaacagactc tttaagttaa gcgggatact ttatctttgg gctactcaaa agcagacagg
     9601 atgttactat gactcaaaat atcaggccgt taccccaatt caaatatcat cccaagccac
     9661 tggaaacagg cgcatttgaa caggataaaa ccgtagagtg cgattgctgt gaacaacaga
     9721 cgtcagttta ttactcgggt cccttttatt gcgttgatga agttgaacat ctctgtccgt
     9781 ggtgtattgc ggacggttct gctgctgaaa agtttgcagg tagttttcag gatgatgcca
     9841 gcatagaagg tgttgaattt gagtatgatg aagaggacga atttgccggt attaagaaca
     9901 catatcctga tgaaatgctg aaagagttgg ttgaacgcac gccaggttat catggatggc
     9961 aacaggaatt ctggcttgcg cattgtggcg atttctgtgc ttttatcggc tatgtgggct
    10021 ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct tgaagaagat tgtgagaatt
    10081 tcggtattag aaattctgat ctagctaaat gcctgcaaaa gcgtggtgat tgtcagggtt
    10141 atctcttccg ctgtctccac tgcggcaagc tgagactgtg gggtgatttt tcgtagttat
    10201 ttaaataatg agaacaggcc ggagcgtaat tcatacatcc ggccttattt cttcagctta
    10261 accgagcgct aattctgcgg cagcggcttt atcagcgatt accatcagcg aaggatgcag
    10321 ctgcaacacg gaagctggaa catcttcggt aaccggacct tgcagcacat ttttcagtgc
    10381 ctgcgcttta cctgcgccac tgacaattat cagcaagttt ttcgccgcca tgatgctttt
    10441 cggccccatc gtcacgtagc tgtcaggcac taacgaaaag tcgccgccca actcaccatg
    10501 cgccacaata tcaaccatct ctccctggat cgggaactcc acggtttgct catggaaatg
    10561 ggtcgtattc ggcaaattgc cacaaaaatg accatctgca cctaatccca gcaccaccaa
    10621 atccaggccg ccttcacgcg ccagtttctg atcatgctcg cggtagttat caatagtgag
    10681 cttctggata ttctcttctt tgatccccgc aggggtgaaa aacagattac gcagattggt
    10741 aatcgttaca ccttctccct ctttgccgcg aaagggaatt tcatcgaaat tatagaaata
    10801 gcagttatca taccagggct tacctttaac cagggtggtg aggtattcat acatgccttt
    10861 gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc gtcttcgaca tataaccaag
    10921 cagatggtgt gccgcgacac ggctcatttc ctggtaatct tcggtaatga ttaatttcat
    10981 cttagtgcct tattttattt aaataatttc tgtacgaagg ctgcataggc aggccgccag
    11041 acatccattt catggttcag gccgggatat tcctggtaat caaacttaat ttttttctgc
    11101 tcaagctcag ttttcagccc ggcgatatcc ttgccggtta cgacatcttt atcaccaacg
    11161 accacagtaa aattacgtag ttgctggtta atagctgccg gatcgttcag ccgggccgcg
    11221 acaccttcat ccggtacggt tgtcgtggta acaccactga atgtggccag ccagccaaag
    11281 ctttccagat gattcattcc ggaaaccagc gcctggtacc cgccttgtga aagccctgcc
    11341 agcgcgcgac catcggcatc tttacggaca ttaaaacgct tgctaatcag cgggataata
    11401 tcgttcatca gttcgcgatc tgccgcttta gcattcagcg gataaaagac tttacgtctt
    11461 tcctgaggca cgaaatcttc gggaataatg cccttcgcat cggtttcggt atccgggatc
    11521 accaccagca tcggtttaat tttcccttca gccagcaggt tatccatgat ttgcgggata
    11581 cgcccctgat cgatagcgga acgtccggta tcaccaaaac cgtgatagaa atagagcact
    11641 ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc agacatacat ctgacgttca
    11701 gattgcaatg cgttggagtg gtaagttatg gcgatcaaat caccatgcgc aacagaacgc
    11761 gtatccagat aactgcctgg caccagaatc atactggagt taacctggcg ctgaggctta
    11821 gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa cattgaaaaa atactcgtac
    11881 aggttgcctt tcaggatagg tgtgcgccac gaccagactc ctgcttcgtc tttggtcatc
    11941 gggtgaatat tgtccggaac cggaacaccc accactacag agacattttt tgccccaggg
    12001 gcaaagtagc gaaaggtcac gctgttatcg gcattgacct gagtcacata ctgtttcacc
    12061 gggatagtgg gtgccgggct ggctggcata tccgctgcta tggcccactg agcagaaata
    12121 ccgctggcaa tagccagcgt taaagcagca atttttatat tcatcgtaat attcctttca
    12181 ttaagttcca ttaccaccag atttcagcct gggcaccgac agcaaactgg tcgtctttat
    12241 tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc tttgatatac gtggcgtaga
    12301 aacgaatttc cggacgcgag gtcaacatac tggtattgac tttaaaggta tggaagagcg
    12361 tggttttata accagactca ttaaatttat tactattcgc atctttgttt tgctgggtga
    12421 aatagcccag ctcaacacct gtctggttat attggtccca aatataggcc gggcgaacaa
    12481 ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc gtagctataa atatcgttac
    12541 cgaaggagta aacaatcgcg ttagccacaa tgaaatgatc gccgatatag gcttcgccct
    12601 gcgaagtcag acgaactgct gttccgccgg tgtgatcacc ataataacga ccattaaagg
    12661 tagtaaatgg acttgcgcca gcataacggc caaagttact ggcgatagaa ttattcgcga
    12721 ccaggaagga gaattcgttg aagccacctt tatcaaattt ctgcgttaaa gatgtgccaa
    12781 atatccaggt atctttccag tcataatacc cgttattatc ctgattatct ttttccgatg
    12841 cactttcgtt tgcagtgaca taacgaccgc tcaccattaa ggtggcttta tcccataacg
    12901 ggatatcttt atagcgtaaa tcaatggtat tagtatttat ctgctgtttg ttttgcaggc
    12961 tgcgatcgta gtcatcgaca tcttcacgaa ccagcgcgac atcaatttta cccggaccga
    13021 ctttccagtt ttccagacct acacctgcgg cagcatcggt acgctgggtt ttccagtcaa
    13081 gcatctggat ttcaattttc ggcgcaccat gtttacccac ccagaaatca gcctctggcg
    13141 caaagggcag gaaacctttg gtggtaacgt acatatcgga gaactgcata aagttctcgc
    13201 caccggcgtt atcgccaaac cagccggtag agtactgctg accaacgtta ccatccatca
    13261 tcacaacggc atcaacccgt ttgccgtttt cgttgtagac acgttgtttt aactgcaaat
    13321 caaaccagcc ggagtattcg ttaccaaagc ggcccagaga accaatcgcc catgatttag
    13381 gtgaaccatg agaggcggtc ccccaaccag aacggtaata gccgttgtaa ctaaagccaa
    13441 tttcatcttt cacaaactta ctgaaatctt tcagagtcat ggaagaataa atcgagcctg
    13501 tctcactggc atttttctct tcatttttga ccagtacagc atcaggtttt gcggcactac
    13561 tggtcgggaa cggattggcg gcataccctt gatcattcgt acttacgcta cgattcaccg
    13621 tcgctggcgt atattttttc ttctcttcat ctttatactt tttgagttcg ctttgcgttt
    13681 ctcttaacgc tttttctaat aactcaaggc gatgttccac cgttaatgat tcagccaatg
    13741 attgtgcaga aaaggctaac ggtgccatta ataagatggc agaggtaata agatttcgtc
    13801 taaacataat atccctttat ggtgcaaaga aagaattaac gtatcgcatc cagactgttc
    13861 tgaatgcgac gataattaag gtgctttatt ggttattttt ttaatgacag cccccgcgtt
    13921 ttgatcactt ctgcatacca tccgaagctt ttcttacgcg ttcttgtgag gcttccttcg
    13981 ccattatcat cacgatccac ataaataaag ccgtagcgct tagacatttg tgaatgagat
    14041 gcactgacta aatcaattgg cccccaactg gtgtacccca taatatccac accatcggca
    14101 atcgcttcat ttacctgtac caggtgatcg tttaaatagg caattcgata atcgtcctgt
    14161 atcgaaccat ccgcttcaac gctgtctttt gcgcctaatc cgttctcgac aataaataac
    14221 ggtttttgat aacgatccca aagcgtattt aacagaaccc gtaatccaac cgaatcaatt
    14281 tgccaccccc actctgaact tttcagatgc ggattgggga tcatattcag tatgttgccc
    14341 tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac cagtcatgta ataactaaaa
    14401 gagatgaaat cgacggtatg ttttaaatct tctgcgtcac tttcagtcat ctcaatggtg
    14461 atattgtggt cgcggaagaa acgctgcata tagccgggat actggccacg cgcctgaaca
    14521 tcaccaaaga acatccagcg ccggttctct tccatggcct gcaacatatc ctgtggctgg
    14581 caggtgaggg ggtaaaccag cccaccgaga agcatattgc cgattttcgc ttcggggagc
    14641 aggctatgac aggctttaac tgcccgcgca ctggcaacca gttgatggtg gatagcctga
    14701 taaacttccg cctcgccact ctcttctgcc agccccacgc ccgtgaatgg ctcgtgtaac
    14761 gacatgttga tttcattaaa cgtcagccat aacgccactt tatgttggta gcgagtaaag
    14821 accgtgcggg cgtaatgctc gaagtgatcg atgaccgctc gattagccca accgccgtag
    14881 tttttcacca gcccatatgg catttcgtaa tgggataacg ttaccagcgg cttgatcccc
    14941 gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta accccgcttc attcggttcg
    15001 acttcgtcgc cctgagggaa aattcgcgcc caggcaatgg aaatacgcag acaggtgaag
    15061 cccatctcgg caaataacgc gatatcttcc gggtaacggt gataaaaatc gatggcgaca
    15121 tctttgatat tctctttccc caggatgcgc ggttccattt ttcccattac gccatgaggc
    15181 tgtaaatctg aggtcgagat ccctttgcca tcttcctgcc aggcaccttc cacctgattg
    15241 gcagctgttg cgccacccca aagaaatgtt tctggaaatg ctttcataat taactccttt
    15301 tatcgttagc gaatgatgga taacagcggt tcacctgcgc ttatctgcgc cgtgccgtgg
    15361 ggtaatacgt ccgtaaaatc atcgctatta ctgattaata ccggcgtcgt cagatcaaat
    15421 ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc gatcgcctgt attgaccttg
    15481 tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca gttttacggt gtcgataccg
    15541 acatgaatca ggagctccac accatcatct gactcaatgc caatggcgtg taatgtggcg
    15601 aacaacgaag caattcgacc cgcaaccgga gaacgcactt caccaaccga gggcagaatg
    15661 gcgatacctt tacccaacag gccactggca aacgtggtat cagcgacgtg aatgagcggc
    15721 acaatctctc ccgtcatcgg tgaacagata ccgccctgct caggtggtgt aataacctct
    15781 ggtgttttct cttgcggggc accctgcgct ggctgacgtt tagcggtgat gaaatgaagc
    15841 atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac cgccaataac gctggcccag
    15901 acggtgaaat caattcccgt tgacgggatg gtttgcatga aggtgaaaat acttggcaaa
    15961 ccaaaggagt agactttcgt ttgcgcgtag ccaatgatgg tggcccccaa agccccactg
    16021 atacaggcga taacaaaggg gtacttacgc ggcagattaa cgccatatac cgctggttcg
    16081 gtgataccaa acaggcccgt caacgccgct gatcccgcca ccactttttt ctgcgcatcg
    16141 cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg ccataatggc gggcattaac
    16201 agcgggatca tggtgtcgta gcccagcacg gtgaagttat tgatacatag cggcaccagg
    16261 ccccagtgca gtccgaacat gacgaagatt tgccagaagc cgcccattac cgcgcctgca
    16321 aatgcaggaa ccgcctgata aagccagaga taaccggcgg caatcagttc gcttatccag
    16381 gttgatagcg gccccaccag cagaaaggtg acgggtgtga taaccatcag acatagcaat
    16441 ggtgtgaaga aatttttgat tgccgacggt aaccacgcat taagtcggcg ttccagaatg
    16501 ctgcacaacc aggcagaaaa aataatggga ataaccgatg acgagtaatt caacaatgtg
    16561 accggaatac ccaggaaatc cagccccagc tcatccgctt tttgcccgtt ctcgaaagca
    16621 gtcagaatta atggatgcac taacgctcca ccaatcacca tggcagtaaa tggattaccg
    16681 ccgaagcgtt tccccgcggt gtatcccagg attatcggga agaaccaaaa caaggcatca
    16741 ctggcgctga ataaaattaa ataagtacca ctttgttcgg tcgtccactg aaaagtgagc
    16801 gccagagcca gcataccttt caggatcccg gttgccgcca tcaaaccgat cagaggcgta
    16861 aaaatacctg aaataacata aacaaagcgg tttagcagat tacctttctc atcattttcc
    16921 ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt tgaccgccag gaagacatcg
    16981 gccacatggt tacctatgac cacctgaaac tgaccaccgc tttccaccac cataataata
    17041 ccgggggtct ttttcagtac ctcagcttgc gctttgcttt catcctttaa cttaaaacgt
    17101 aatcgcgttg cgcaatgcat cagactcaca atgttatctg cgcccccgac tcctgcgact
    17161 atttttctgg ctaactccgt cataacttgc cctctaccgc tttgcggcaa aactccaaaa
    17221 aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt tttttcaggt tttgcccgct
    17281 tagtgcggta acaatccttt actcagtaat aatatttcag tgttctttgc gcacgcgctc
    17341 tatatttatg gctaaaaaca taatctctgc gggtgaaatt ttacgttgat actgcaaacc
    17401 aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc gggtaatttt gttttactgc
    17461 ttgttgtaat gattcatcac tatcgttaat tgaagcatgt tcaagaatac gccaggataa
    17521 aaacttcaga tgtgtaacca gtcgctgata actcaagctt tcttcctggt aattaaggct
    17581 gaactgaaat tttattaatt gcagcatttc gcgcattaac tgcgtgacac ctgcaacatc
    17641 ctccatattt ccgctcattt gggcactgac cagatgcatg gcaataaagc ccacttcatc
    17701 tttcggtaac tgcacgccca accgtttatc aataatggtt aacgcttctt cccctagctg
    17761 gaactctttc gggtaaagcc gctggatatc ccacagcaac gggttgggca gcaacacgtt
    17821 ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat aaatactgtc
    17881 ctgtaatttt cccaagcgct cctgcgctaa agagataata cgatcacagg ttgccatcac
    17941 ctcaagagga atatgactta agagttcgct taatcgcccg ttcagttcat gactgctcaa
    18001 ggcatactct ttttctattc cacttgagtt aattctttcg ccagcgcgtt tttgaaagcc
    18061 aattcctcgc cccatgacga ctttttcccg ctgttgatca tcaataacca ccacaacatt
    18121 attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta taaacaaaaa
    18181 aacccgactt caccagtatt ctctggttat gtcaggtttt gcctgcgaat gcagtaacaa
    18241 tccagtcatt tattaatggt ttttataacg aacatccagg ttcggaaatt aatttaattg
    18301 cgtgcagaga aaatagcaat gcgctattga
//
LOCUS       DAPPNJ010000086        18249 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000086 DAPPNJ010000000
VERSION     DAPPNJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.086"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..548
                     /locus_tag="RPM73_002426"
                     /pseudo
     CDS             1..548
                     /locus_tag="RPM73_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            651..1154
                     /locus_tag="RPM73_002427"
     CDS             651..1154
                     /locus_tag="RPM73_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDX6520775.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1221..2678)
                     /gene="nuoN"
                     /locus_tag="RPM73_002428"
     CDS             complement(1221..2678)
                     /gene="nuoN"
                     /locus_tag="RPM73_002428"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDX6520776.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2685..4214)
                     /gene="nuoM"
                     /locus_tag="RPM73_002429"
     CDS             complement(2685..4214)
                     /gene="nuoM"
                     /locus_tag="RPM73_002429"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDX6520777.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQTSGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4378..6219)
                     /gene="nuoL"
                     /locus_tag="RPM73_002430"
     CDS             complement(4378..6219)
                     /gene="nuoL"
                     /locus_tag="RPM73_002430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDX6520778.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6216..6518)
                     /gene="nuoK"
                     /locus_tag="RPM73_002431"
     CDS             complement(6216..6518)
                     /gene="nuoK"
                     /locus_tag="RPM73_002431"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDX6520779.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6515..7069)
                     /gene="nuoJ"
                     /locus_tag="RPM73_002432"
     CDS             complement(6515..7069)
                     /gene="nuoJ"
                     /locus_tag="RPM73_002432"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDX6520780.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7081..7623)
                     /gene="nuoI"
                     /locus_tag="RPM73_002433"
     CDS             complement(7081..7623)
                     /gene="nuoI"
                     /locus_tag="RPM73_002433"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDX6520781.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7638..8615)
                     /gene="nuoH"
                     /locus_tag="RPM73_002434"
     CDS             complement(7638..8615)
                     /gene="nuoH"
                     /locus_tag="RPM73_002434"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDX6520782.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8612..11338)
                     /gene="nuoG"
                     /locus_tag="RPM73_002435"
     CDS             complement(8612..11338)
                     /gene="nuoG"
                     /locus_tag="RPM73_002435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDX6520783.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11391..12728)
                     /gene="nuoF"
                     /locus_tag="RPM73_002436"
     CDS             complement(11391..12728)
                     /gene="nuoF"
                     /locus_tag="RPM73_002436"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDX6520784.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12725..13225)
                     /gene="nuoE"
                     /locus_tag="RPM73_002437"
     CDS             complement(12725..13225)
                     /gene="nuoE"
                     /locus_tag="RPM73_002437"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDX6520785.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13228..15030)
                     /gene="nuoC"
                     /locus_tag="RPM73_002438"
     CDS             complement(13228..15030)
                     /gene="nuoC"
                     /locus_tag="RPM73_002438"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDX6520786.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15124..15786)
                     /gene="nuoB"
                     /locus_tag="RPM73_002439"
     CDS             complement(15124..15786)
                     /gene="nuoB"
                     /locus_tag="RPM73_002439"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDX6520787.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15802..16245)
                     /gene="nuoA"
                     /locus_tag="RPM73_002440"
     CDS             complement(15802..16245)
                     /gene="nuoA"
                     /locus_tag="RPM73_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDX6520788.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16876..17814)
                     /gene="lrhA"
                     /locus_tag="RPM73_002441"
     CDS             complement(16876..17814)
                     /gene="lrhA"
                     /locus_tag="RPM73_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDX6520789.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4562 a         5081 c         4705 g         3901 t
ORIGIN      
        1 gccaacgccc cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca
       61 aactgtgcat ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat
      121 aaaagagttc tttgtcagta acggtgccag agttaccgcg cagggcgtcc ccgattggct
      181 attacgactc tgcgcctggt agccaggatt acccttaccc gcaatgtcct ggctttatcc
      241 cgatcggggg gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag
      301 gtaaaaacgg cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg
      361 tttattcccg gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc
      421 gtaatgatta ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca
      481 gcagacagat tgaattatca gaaaatggct cagatggcgt tataaccttg ttacacaaca
      541 gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc
      601 cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga
      661 ttggttattt tgcagaaatt gactcagaaa agattaacca actgcttgag tctactgaaa
      721 aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg gatattgata
      781 aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat cccgcgaaga
      841 atgatccgct gagtcgtgcc gtgcttggtg aacacagcct cgaagatggc atagatggct
      901 tcctgggatt gacctggaat caggagttag ccgcgacaat cgaccgactg gaatcactgg
      961 atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg gagatttatc
     1021 cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcttcgatc atgcttgata
     1081 tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat gcgctgacgg
     1141 ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg ccgaacatgt cggcggcttt
     1201 ctgacttaca aactgatagc ttacatcagc ggcattgcta accgcacaat gctaatcagc
     1261 ggttgtggcc atacacccag caccagtacc aacagtgcag agatcagcac cacgataccg
     1321 cctgcgctgt actgccagtt tgacggtgca tcgcgacccg gttgttccgg ggcgtgcagg
     1381 tacaggctca ccgccacgcg caggtagtag taaaggccga ttgccgaacc gacaaccacg
     1441 gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttacca
     1501 ataaagccca gcgtcatcgg aataccggcc agagacagca tcatcaccgt catcaccgcc
     1561 gcgaggatcg ggcgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg
     1621 ccacggtacg ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga
     1681 taaccggcca ggtaaactcc taccgcttcc atcgacatct cgcccgtttg cagcgcaatc
     1741 agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag acgtttgata
     1801 ttggtctggc tcagcgccat caggttaccg aagatgatag aggcaaaggc gataatcgcc
     1861 agcactaccc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag acgcatcacc
     1921 acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg
     1981 ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg
     2041 ccaacaatca tcagtccgaa acctgccagc aacagcggct cgttgagcat accgtcgccg
     2101 aggtttttac ccagcgcgac aaacgacagg tcgccagact gtgcatacac cagcgccata
     2161 ccaaacagca ggaaagaaga cgctgcggca gaaagaatgg tgtatttgat actggcttcc
     2221 agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt
     2281 tcaataccga ggaacaaaga cgccagatgg ttggcattcg ccagcaggat cccgcccagc
     2341 gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc ttcaagccac
     2401 gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg
     2461 gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg gccgacaaac
     2521 cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga
     2581 ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac gatcagcaac
     2641 ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta
     2701 gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga
     2761 atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac
     2821 agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg
     2881 aagtaagcgc gatgtaacat cgccagcgaa taaacagatg caaagaccag cccaaaggta
     2941 gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg
     3001 acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag cgacagtgct
     3061 ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata
     3121 cgttcataaa gctgaccaca aagaataaac agacccgccg ccgacaagcc gtgcgcaatc
     3181 atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagatagc aatcagcacg
     3241 aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag
     3301 gccatccacg caccgtagaa gatgccgata acacccagcc acatagcaat tggcgcgaac
     3361 tctgccgacg cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta agcggcagtt
     3421 ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg gagcctggga gtgcgcatcc
     3481 ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat gaagaagccc
     3541 agcattaaca ggtattccac accactggac attggcgtat tcagcagctc ttcatagttg
     3601 aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcaatcaac
     3661 atcaccagac cactcgtctg ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt
     3721 ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc
     3781 atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg
     3841 cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac
     3901 gaacagagta ccgccagcac accgagcaga ccggtcagca cgaccatcag cagcgacagc
     3961 ccgtcaatgg cgagatggat agagatacca aaacgcggga tccacggcat gtcgaattca
     4021 gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt
     4081 tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg cggcaccttg
     4141 acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat taatattagc
     4201 cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca
     4261 acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat gcgccgcgaa
     4321 ggcagcttat ccgacctacg ttcttatatg cccaggggca atcccacaat ccttaactca
     4381 acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata
     4441 ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc gggaaagaac
     4501 cgccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag
     4561 gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca
     4621 ggtactcagc aggcggcccg gcgcactgtt ggcgatggag gtcaccagag tacgtttacc
     4681 cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc
     4741 cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc cctgcagcgg
     4801 cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct
     4861 gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa
     4921 aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg ccaccatcag
     4981 attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc
     5041 agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga gataaaccag
     5101 cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag
     5161 aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg
     5221 gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct
     5281 catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg caaaaccggc
     5341 cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg gcgtcatcag
     5401 gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg ttgcggcgtg
     5461 gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg tctgcaacgg
     5521 caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat
     5581 gttattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt
     5641 gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac ccacacgggt
     5701 cacgacgaac gctttcattg ccgctgcgcc attcttcgga tcggtgtaat agaaaccgat
     5761 cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttatc
     5821 ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga agaagcgaga
     5881 gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga aacccacacc
     5941 agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt
     6001 aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct cgccgttagc
     6061 gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag agccaacgcc
     6121 gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc
     6181 aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa
     6241 tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag gccgatactc
     6301 gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctgaccc
     6361 cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca
     6421 atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag aacgaataag
     6481 attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt
     6541 tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt
     6601 ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt
     6661 aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct
     6721 gatcattaac accaaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg
     6781 ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc
     6841 ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga
     6901 taatttccag cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg gaaatcgcca
     6961 gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca
     7021 aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc
     7081 ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc
     7141 tttgccgtcg attgccatac ctgccatccg gtagaagtta tattctgggt atttgcccgg
     7201 accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt attcccccat
     7261 ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca
     7321 gaaaatgcag cgtgagaagt tgatgcggaa aaattccggg taccagcgac cgtctttggt
     7381 ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcgcaga ggttacaggc
     7441 tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg
     7501 cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag
     7561 gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt cttttaaggt
     7621 catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag
     7681 ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc
     7741 ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcagc
     7801 gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcacc
     7861 atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag accgaacttc
     7921 ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca
     7981 aacgggtgac ggtgacatac cgccacgccc gcgatggcaa aggtaataaa accaaagaat
     8041 tgcgggataa cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa
     8101 ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagggtc
     8161 tgcgcagaag cacgcatcgc acccagcaac gagtatttgt tgttacttga ccagcccgca
     8221 aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat cccgatgttc
     8281 aggtcggcaa ccacccaacc cggactgact ggcacaatcg caaaggccag cagcagcgag
     8341 gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag
     8401 tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc gccccagcca
     8461 acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc gccaaagctc
     8521 atgaatgccc cgcaggtgac aaccaccagc aggatcacca ccgctttgag gatggtcagc
     8581 aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa
     8641 gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaaa cccacctgcc
     8701 ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa
     8761 aggagacgcg agcgcctgcg ttcacgccca acttcgcggc atccgctggg ttgagtttga
     8821 tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc
     8881 caaacaggtg gtaatacggc gcgatacgcc atttcccgtc ctgcggctgg aagcgtgccg
     8941 gtacgctggt gaagtaatcc agaccatttt cgctggtttc aaacagacgc acgcccggat
     9001 cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc tgcggggagt
     9061 tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcagtcggc tggttgttac
     9121 cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc
     9181 tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct
     9241 gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacctgcc agttccggga
     9301 ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact tcacggctca
     9361 gcagggtgct gtgcagcgag tgtaaccagc gccagctttc cagcatgaca gttttgctgt
     9421 cgtaataagc aggatcgtaa acctggaaga aacgttgggc gcggccttcg ttgttgatca
     9481 ccgtaccgtc gctttcagca aagctggcag cagaaagtac cagatgggcg ttttccataa
     9541 tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc gcagcattca
     9601 cgcgggtagc ggaagcgtga cgatgcagat cgttttccag caccaccacc gcgtcggcgc
     9661 gtccggtttc cagttcggtc aacgcttctt caagcgaacc gccacccata atgcccagcc
     9721 ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg cgacctttca
     9781 gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg ttcgtcccgg
     9841 agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg attttgcttt
     9901 gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt atccagcgca tgggcgatgg
     9961 caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat
    10021 ccagacgggt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt
    10081 tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt
    10141 tacctttcac agcctgacgc actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt
    10201 cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga gtataaatgc
    10261 cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac
    10321 cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt tccacgctgg
    10381 cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct gctgcaccct
    10441 gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt actggctgac
    10501 gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt
    10561 agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgcca taacgctcgc
    10621 cgggactgat gttacagccg atagaacatt gctggcagat gctcggcgca aactgcatat
    10681 cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa
    10741 tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt
    10801 agacgttgtc gtgcgcaccg taaacgccca gatctgtacc gtcagcgtaa tctttgtagt
    10861 aacgcacgca acggtagcag gcgatgcagc ggttcatttc gtgagagatg aatggcccca
    10921 aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca
    10981 tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg
    11041 ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgcc tcttcgtcgt
    11101 caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac
    11161 gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc
    11221 ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca
    11281 gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta
    11341 gcatgcttcc agttttctca gtcagagact gagcgttaat cgaaattcgg ttaccagcgc
    11401 tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg
    11461 attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca
    11521 ccaggcgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt
    11581 gtttcgatat cgcccggctg accttcacca cgctccagcg cacgcagaat tttcacgctc
    11641 cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc gaaaaactct
    11701 tccagattac gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc
    11761 gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag gtcaaggtgc
    11821 gcttcagtca ggaaatcagt ccccgcgccg cctggctgcc aggctttgaa tttcagaccg
    11881 tcacgcatac caccggcgta atcttcaagg atttcacgcg cggtagtacc gaacggcagt
    11941 tcccacaggc ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct
    12001 ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta
    12061 cacagggttt cgacgttgtt gacacaggtc ggtttacccc atgcgccgga ggtcgccggg
    12121 aaggggggct tcgagcgtgg gttagcacga cgtccttcca gggagttgat taacgctgtt
    12181 tcttcgccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcaaaacct
    12241 gttcccataa tatttttgcc aagcagacct gcttcggtgg cttcggcaat ggcacggcgt
    12301 agattcactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc
    12361 agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg ctccatcaac
    12421 aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag gtaacggatg
    12481 ttcatggatt cgtctttcgg catcaggctc catttcaggc cggtagaaaa gcccgcgcca
    12541 ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg agacagcccg
    12601 gtcagcgcct tacgcgcgcc ttcgtaaccg tttttgctgc gatattcgtc cagccacact
    12661 ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg
    12721 tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag
    12781 tatcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca
    12841 gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt gatgtttagc tttttctcga
    12901 gcgccgcctg aataccctgg taaccgttga tatgacagac cacgctgtcg caataacgga
    12961 tcacatggcg gccaaccggc tggcggaaga tctggctgta gaacgttgcc acaccttcga
    13021 cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc
    13081 agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt
    13141 agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg
    13201 tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat
    13261 actgcccaga taaacaatca ggtcagacac caggctaccg cggatcgccg ccggaatttg
    13321 ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg tgctaccgtc
    13381 gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt
    13441 ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg
    13501 ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc
    13561 ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca gctcttccac
    13621 tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga tttcaaagtc
    13681 gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc
    13741 acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac
    13801 gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt aagacgccag
    13861 acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccgc gcggcaggtc
    13921 gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc
    13981 caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc
    14041 gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga acagttcgga
    14101 gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac
    14161 cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac ggtcggtata
    14221 cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac ggtggtggta
    14281 gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa
    14341 agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt cctcgttttc
    14401 ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc
    14461 ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt
    14521 acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc
    14581 gtcgaaagta atgccaaaca gatcccaggt ttcacgctca taccagttag cgttcgggaa
    14641 cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcagcatgat
    14701 gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa
    14761 cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta
    14821 aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca
    14881 cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt
    14941 gcgcagttcg ccaatcaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg
    15001 cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca
    15061 gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc
    15121 aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg
    15181 ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag
    15241 cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc
    15301 aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat cgacgccctg
    15361 cacaacggaa taaatatcgt acataccacc agagttggca caggcaccca ttgagataac
    15421 ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa
    15481 gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc
    15541 gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca
    15601 ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt
    15661 gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc
    15721 ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata
    15781 atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt
    15841 catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata
    15901 aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc
    15961 gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac
    16021 gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga
    16081 gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa
    16141 ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa agatagcgaa
    16201 tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa
    16261 agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg
    16321 aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct
    16381 tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca
    16441 taatatcaac accattagca tttaatgccc tttcacccca gatccttggc gactccagga
    16501 taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt
    16561 tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac
    16621 gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg
    16681 tagcaggtga tttttttcag gcgattattt gtgcgttcgg gatgtgaatc tctggtgatt
    16741 gaaaaatgaa cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa
    16801 gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta
    16861 tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg
    16921 agcagacatt gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac
    16981 ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag
    17041 cggcggtaag ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg
    17101 ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc
    17161 gacataagcc agtcgccatg gaatatctgc tttattaagc gtcgccagca ccatatcgcg
    17221 aaacggactt ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta
    17281 ttccgctgcg cagtaccagt gtgttggcga ggttcgcagg ttcaatgcct taaacgctga
    17341 aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata
    17401 ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt
    17461 taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc
    17521 ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct
    17581 ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc
    17641 gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg
    17701 agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa
    17761 tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta
    17821 tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc
    17881 catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat
    17941 atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca
    18001 gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat
    18061 gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg
    18121 tcatacgcga acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca
    18181 tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc gaattatctg
    18241 atgcaaagt
//
LOCUS       DAPPNJ010000087        18207 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000087 DAPPNJ010000000
VERSION     DAPPNJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.087"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..147)
                     /locus_tag="RPM73_002442"
     CDS             complement(<1..147)
                     /locus_tag="RPM73_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="member of the SPOUT superfamily of
                     methyltransferases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDX6520790.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAH"
     gene            complement(547..687)
                     /locus_tag="RPM73_002443"
     CDS             complement(547..687)
                     /locus_tag="RPM73_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520791.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            783..1499
                     /gene="arcA"
                     /locus_tag="RPM73_002444"
     CDS             783..1499
                     /gene="arcA"
                     /locus_tag="RPM73_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDX6520792.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1559..2911)
                     /gene="creD"
                     /locus_tag="RPM73_002445"
     CDS             complement(1559..2911)
                     /gene="creD"
                     /locus_tag="RPM73_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDX6520793.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2969..4393)
                     /gene="creC"
                     /locus_tag="RPM73_002446"
     CDS             complement(2969..4393)
                     /gene="creC"
                     /locus_tag="RPM73_002446"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDX6520794.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4393..5082)
                     /gene="creB"
                     /locus_tag="RPM73_002447"
     CDS             complement(4393..5082)
                     /gene="creB"
                     /locus_tag="RPM73_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDX6520795.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(5095..5568)
                     /gene="creA"
                     /locus_tag="RPM73_002448"
     CDS             complement(5095..5568)
                     /gene="creA"
                     /locus_tag="RPM73_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDX6520796.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5779..6648
                     /gene="robA"
                     /locus_tag="RPM73_002449"
     CDS             5779..6648
                     /gene="robA"
                     /locus_tag="RPM73_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDX6520797.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6645..7292)
                     /gene="gpmB"
                     /locus_tag="RPM73_002450"
     CDS             complement(6645..7292)
                     /gene="gpmB"
                     /locus_tag="RPM73_002450"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDX6520798.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            7344..7865
                     /gene="yjjX"
                     /locus_tag="RPM73_002451"
     CDS             7344..7865
                     /gene="yjjX"
                     /locus_tag="RPM73_002451"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDX6520799.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7950..8276)
                     /gene="trpR"
                     /locus_tag="RPM73_002452"
     CDS             complement(7950..8276)
                     /gene="trpR"
                     /locus_tag="RPM73_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDX6520800.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8366..10303)
                     /gene="sltY"
                     /locus_tag="RPM73_002453"
     CDS             complement(8366..10303)
                     /gene="sltY"
                     /locus_tag="RPM73_002453"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDX6520801.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10514..12181
                     /gene="ettA"
                     /locus_tag="RPM73_002454"
     CDS             10514..12181
                     /gene="ettA"
                     /locus_tag="RPM73_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDX6520802.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12488..13720)
                     /gene="nadR"
                     /locus_tag="RPM73_002455"
     CDS             complement(12488..13720)
                     /gene="nadR"
                     /locus_tag="RPM73_002455"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDX6520803.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13741..15123)
                     /gene="radA"
                     /locus_tag="RPM73_002456"
     CDS             complement(13741..15123)
                     /gene="radA"
                     /locus_tag="RPM73_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDX6520804.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15172..16140)
                     /gene="serB"
                     /locus_tag="RPM73_002457"
     CDS             complement(15172..16140)
                     /gene="serB"
                     /locus_tag="RPM73_002457"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDX6520805.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            16246..16890
                     /locus_tag="RPM73_002458"
     CDS             16246..16890
                     /locus_tag="RPM73_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDX6520806.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16918..17934
                     /gene="lplA"
                     /locus_tag="RPM73_002459"
     CDS             16918..17934
                     /gene="lplA"
                     /locus_tag="RPM73_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDX6520807.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            17966..>18207
                     /locus_tag="RPM73_002460"
     CDS             17966..>18207
                     /locus_tag="RPM73_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6520808.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPS"
BASE COUNT         4356 a         5052 c         4559 g         4240 t
ORIGIN      
        1 atgtgcgacc cagcgggtgg ctggctccag atgtgcctga ctatcgacaa tccgcagttc
       61 gctaaaaccc atcgttttca ttgcccgcgc cgctgcccca atattttctg ctctggcggg
      121 tgcgaccaga ataatcgtta tacgcatatt gccactcttc ttgaccaaat aaccgcgaac
      181 agggtgatca ctgtcaactt attacgcggt gcgaatttac aaattcttaa cgtaagtcgc
      241 agaaaaagcc ctttacttag cttaaaaaag gctaaactat ttcctgactg tactaacggt
      301 tgaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt ataaaaaata
      361 aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag ttggattatt
      421 aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact gttaacgtgc
      481 tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc ggcccctctt
      541 agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca ctgtcgggtc
      601 ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg aaagtgcatc
      661 aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg cagcatgcat
      721 caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt taggtagcaa
      781 acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc aacacgttga
      841 aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc gcggaaatgc
      901 atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat ctgccgggta
      961 agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg ttgatgttcc
     1021 tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt gcagatgact
     1081 acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac ctgctgtccc
     1141 gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc tacaagttca
     1201 atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc gagcagtaca
     1261 agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca ggcaaaattc
     1321 agtctcgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaacca cacgaccgta
     1381 ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg gatacgccgg
     1441 aaatcatcgc caccatccac ggtgaaggtt atcgcttctg tggtgatctg gaagattaat
     1501 cggctttacc accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt
     1561 atttccagat acgtaattga tcgtccattg taacttcttt actggctttc attttcggca
     1621 gtgaaaacgc ataccagtcg atattacgag tcacaaacat catgccggcc agcgccacca
     1681 ccagcacact ggttcccaac aacagcgcgc tatcggcaga gttgagcagt ccccacatca
     1741 caccatccag caacaacagc gcgagggtaa acaacatgct gttgcgccag cctttcaata
     1801 ccgcctgcaa ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca
     1861 cggtaaaacc gatatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa
     1921 gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa
     1981 agaacgccat aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc cggtccgtta
     2041 attggtattg atcggctggc gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc
     2101 cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag ctgctttgcc
     2161 attgcgcctg aaaacctgac tcgctaactt cccgtttggc tggtagaaaa tcacctaaaa
     2221 aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg gcaggcacca
     2281 cagaaagatc gccggtaccg cttaaattca gtgccatatt cagctgcagg ttctgcttcc
     2341 gccagtcccc ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtac
     2401 cgggttcaat ggtcagtgcc gttccgttaa cttcaggcgc tttcaccaca ccaataccac
     2461 gcgcatcccc gacgctaatc acaataaagg gcttgcctaa gatgatattt ggcgcgttga
     2521 gctcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct
     2581 gaccggtata aatccctatc ttgcgttctt ccacgttctg attgccatca accatcaatg
     2641 actcaggtaa ccaaaaatgg ataaaacttc gtttccgctc cacggtttta tcctcttcct
     2701 gcaccgtata aagctcggtc acaggaatag cgataagcgg cccaacgagt ttttgcggcc
     2761 cgctggtact ttggcgaatc gcatcttcca catcgctacg gtaatcggca cgttcgacaa
     2821 tcacttgccg aatcagcatt atcggaatca acaacagcaa tactgcacca aacagggtgg
     2881 tcattttcca gaacaggggg gatttcaaca tagtcttctc ctttgcaatg gcagcaggat
     2941 acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt cgaagcgagg
     3001 ccagcacgcc accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga
     3061 cctcactgac gaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag
     3121 agtaaaagcg ttcaaaaata cgtgacagcg cgtagtcagg aataccacta ccggtatcca
     3181 gcaccttaag cgtgacgtgt tcctgatcca cttcggcgct tagcgttata cgaccgctct
     3241 cgggggtaaa atcgatggcg ttatccagta aattccccag cgcctgatcc agtaacgccg
     3301 gttcagcagc aacattaacc tcggtgggcg taacatgtag agtgatgttt ttttctgcca
     3361 actgcacggt gcgcgcttcg ctgacgcggc ggaataatgc cgccacatca acagcagtca
     3421 aaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttcc accagtgcct
     3481 gcattcgcgc attttgcgtc agaatgttgt cagtaaaacg agccactact tcaggcggcg
     3541 gaccttcgcg taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat
     3601 gggttaacgc ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac
     3661 tttccagcgc ctgcgcgagt ttacgcagct cgctactacc gagatcggga agaggaacgg
     3721 gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatggag cggttgatcc
     3781 accaaaccat gcctgcgcca atcaccagtg cgatccccaa caaaatggcg ctggcccata
     3841 aaattcgccg ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc
     3901 tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac atcaccgaac
     3961 tttcgggatc ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta
     4021 gccagacgtc attccagcgc gaataatcct gcccaacggc tttatttgcc gaatcgaaca
     4081 atactttacc ctgcgaatcg gtcatataga catgatattc attgcgcact ttgttaatgc
     4141 caccgatatt ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc
     4201 catgcgttgg gtccccagag agcaaatcgg gacgcgccag ctccgccagc aacgttgcgg
     4261 tgtcgatcaa cgtcccctcc gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa
     4321 tagccagtac gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc agcaaccgca
     4381 tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag tattaatcgg
     4441 tgaaagatcg ggattgatgg cgcgcagctt ggcacgcaac gttttaatgt gggtatcaac
     4501 ggtgcgatca taggtgtcct gcgcatcttc ccacacgcta tccatcagtt gctggcggga
     4561 ccagacgcgg cctggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag
     4621 cgttaatggc gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt caaaatgacc
     4681 aatacggatg acgggagacg gcgacgagaa cttcttcacc cgacgcagta aggtgcgcac
     4741 cctggcgcac acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc
     4801 cagcccaagc aggcgatcga cctcttcact tcgggccgtc aggaacaata caggtaacgc
     4861 cggatggagc gccagtaact ggcggcacaa ttcaaagccg ctgatatcag gcaaaccaac
     4921 gtcgagaatc atgacgtcgg gaaccagctg gcgagcttta tccagcaccg gcaagcctcg
     4981 ctcaaagacc tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg tgtcggctat
     5041 cccttgctca tcttccacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg
     5101 ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt
     5161 gtcggagtaa gccagataag cgagcgcgtt gcgtttagca tcataaaagc gcacgacctg
     5221 taacgactta aagaccaggg acgtacgttt tttgaatact acctcgccct gagctttgcc
     5281 gtttttaata cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa tggccgcatc
     5341 ggaggtatct tccgccaggc ccaatccccc tttaataccc ccggttttcg cccggctcac
     5401 ataacaggtg acatttttca catcgggatc gtcaaaggct tccacaacaa ttttgtgatc
     5461 cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg
     5521 ccccagcatt atcaggctta aagacaggat caaatgcttg tatttcatat tgttaccatt
     5581 tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat taagaacaaa
     5641 atctcaatac ttttatttcc gtcaagccct aaaacatact ctactaagaa aaaaacactg
     5701 aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg ctcgttgttg
     5761 aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt aatctggctg
     5821 gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc aggttattcc
     5881 aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg cgcgtatatt
     5941 cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg tccgattctg
     6001 gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc attcaagaag
     6061 cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc ctttggtatt
     6121 cgcccgccgc tacgtctggg tgaattcaca atgccagagc acaaatttgt caccctggaa
     6181 gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca aatctctgat
     6241 ttccgccatg aaatgcgtta tcagttctgg cacgattttc tcggcaacgc gccgaccatt
     6301 ccgccggtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga cgacgaacaa
     6361 gaggtattct ataccaccgc gttagcccag gatcaggcag atggctatgt actgacgggg
     6421 catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg tctgggaacc
     6481 ggcgtgcagg agtttatcct gacggtatac ggaacgtgca tgccaatgct caacctgacg
     6541 cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagacgccaa agcgggagat
     6601 cgcccaatta atctgcgctg tgaactgctg attcctatcc gccgttaacg ctgcagctca
     6661 tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaacgac ccagccagac
     6721 gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg cagacgtaag
     6781 cggcgctctg cccatgctgg taatccgaga atcgtactca ccagacatcc cagtgcaata
     6841 ccatggctca ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc
     6901 gcattgacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg cccgtcaacg
     6961 gtgccattga ccagctgccg acgccagttc tcttcttctt cggtcagaga atcgatatgt
     7021 cttttttcca gcacacccat gtttaattca cgcaggcgag aatcaaagat gatgtcacag
     7081 ccgcaggcct gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc gctactgata
     7141 atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg ttgctcacct
     7201 ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt ccactgcgtt
     7261 tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact gctgtaacgt
     7321 taattttctg agtaatgctg attatgcacc aagttgtctg tgcgaccacc aatcccgcta
     7381 aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc tgccatattg
     7441 catccgttgc cgtcgagagc ggtgtaccgg aacagccctt tggcagtgag gaaacgcgcg
     7501 ctggcgcacg aaatcgggta gcaaatgccc gccgtttact tccagaggct gatttttggg
     7561 tggcgattga agctggcatc gatggtgaca gcactttcag ttgggtggtg attgaaaaca
     7621 ccagccagcg cggcgaggcg cgttcggcta ctttgccgtt acctgcggtg attctgcaga
     7681 aagtgcgtga aggtgaggcg cttggtcccg tgatgtcgcg ttataccggt attgatgaga
     7741 ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact cgcgccagcg
     7801 tctatcacca ggcggtgatc ctggcgctga gtccgtttca caatgccgtg tatcaggcat
     7861 tgtaggacgg ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg cctgatgcga
     7921 cgctgccgcg tcttatcagg cctacaaaat caatcgcttt tcagcaacac ctcttccagc
     7981 cactggcgca gctccacggg cgcggctttc aggctgttag atccacgcgt aatcgtcgcg
     8041 atgcccgcgc cgagttcatt ttttaactca cgctggctca tttcgccgcg caacagctct
     8101 tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag catcagattt
     8161 aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac aaaacgtaac
     8221 cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg ggccataata
     8281 tgtcgccatt gttagcgggg gaagcaaaat gcctcccccg gttgtactcg ctaaagagta
     8341 cgatagcata tcataaacgt gcggatcagt aacgacgtcc ccattccgtg gcgctcatca
     8401 acgtcggttt atcccccatg aaatagcggt agtaagcgtc ataagccagc acgttcttca
     8461 cataaccgcg cgtctcggag aacggaatac tctcgacaaa tgccactgcg tcgatacgcc
     8521 cggcgctgtt gccaagccag gttcgcaccc gccctggtcc ggcgttataa gctgctgagg
     8581 agaaaatacg gttattgcca aactgctgat aaacatattg cagataactg gtgccaatgt
     8641 tgatattcgt ttccggatcc agcaattgcc cagggctact ataaccggga atagagaaca
     8701 tcttcaccgt atgggtcgct gtaccaggca taatctgcat caggccgctg gcccctaccg
     8761 gtgatttcac ttttggattc caggcgctct cctgacgggc aatcgccatc gcatagcttt
     8821 gcgggatctc cttaccgctg gtgtagcgtt tgaaaagatc gttgtaagcc agcgggaatc
     8881 gctcttccag atgatcccat agcttcccgg cgatcgttgc ctgaacgcta agatcccacc
     8941 attggttgtt gaaagcatac cgcgccagtt gagcctgctc tgtttttgac ttgctcttca
     9001 ccagattggc ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc aactcgcgca
     9061 cgcgcgccat ctccggcccc tgagtcaggg cgctgtcaac attctgcggc gctttatcaa
     9121 tcttcagctc atactcttct ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct
     9181 gttgcatgag ttgatgcaaa atctctttcg cttcagcttc acgtccgcgt tccagcaata
     9241 aatccgcctg ccagtaacgc cattcatctt tctcttttgc ttccatcggc agacgcgcca
     9301 gccaggtatt caggccgcga cgatcgccgg tgccaagtgc cattcgcaca cggcgttcaa
     9361 tcaacgacgt ggattgcgag cgcataatgg cgtcatcgcg ccatttcgcc tgctcgtcgg
     9421 tgacatcgtt gcccatcaaa cgccaggcga cgatatcgcg cagctcctga atctgatctt
     9481 cattaagctg ctgcgcctgg gcaagcgacg ggatcatcag ccgtgcattt tcagcatcct
     9541 gccgcgccac actggcaaac gccaccgctg ccatttgacg ggtaaaatcg gtcgctccgg
     9601 ttgtacgcgc gaaggtcagt accgtattag ggttgttcgc cagtgaaatg attgccgagg
     9661 cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg ccagtgttac
     9721 ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt ttacctgacg
     9781 cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg gttagccaca
     9841 gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc gcatagtagt
     9901 aattacattg cgcttcggta gttccgggct tttccgggct aaaggctaac aagccacgcc
     9961 agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga gcgggaggaa
    10021 gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc atcagatcat
    10081 cggtgatctg gcggtattcc aggtagggat aaagcggata atccttcagt ccaggcatca
    10141 tttgttcgac cacatccatt tgtcgattat cccaggcctg cttgatttgc gcgtaacggc
    10201 tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc agacagacac
    10261 cggcagccaa cagccgccag gtaacttgtt tggctttttc cacaagcgca tcctctaagt
    10321 gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca gtcattgccg
    10381 tttacgccac gttacggaca cttttttact tttactgcga aggtgatcga ccaactggga
    10441 ttaggcgggg aaaaaggcta cacttcgcct ttgaaaactc atcatcacga taaacataga
    10501 ggcgaagtcc aacgtggctc aattcgttta taccatgcat cgtgtcggca aagttgttcc
    10561 gccgaaacgt catattttga aaaacatctc tctgagtttc ttccctgggg caaaaattgg
    10621 tgtcctgggt ctgaacggcg cgggtaagtc taccctgctg cgcattatgg cgggcattga
    10681 taaagacatc gaaggtgaag cgcgtccgca gccagacatc aagattggtt acctgccgca
    10741 ggaaccgcag ctgaacccgg aacacaccgt gcgtgagtcc attgaagaag cggtttctga
    10801 agtggttaac gccctgaaac gcctggatga agtgtatgcg ctgtacgccg atccggatgc
    10861 cgattttgac aagctggccg ctgaacaagg ccgtctggaa gagatcattc aggctcacga
    10921 cggtcataac ctgaacgtac agctggagcg tgcggcggat gcgctgcgtc tgccagattg
    10981 ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct
    11041 gcttctggaa aaaccagaca tgctgctgct cgacgaaccg accaaccacc tggatgccga
    11101 atccgtggcc tggctggaac gcttcctgca cgacttcgaa ggcaccgttg tggcgattac
    11161 tcacgaccgt tacttcctcg ataacgttgc aggctggatc ctcgaacttg accgcggtga
    11221 aggtattccg tgggaaggca actactcctc ctggctggag cagaaagatc agcgcctggc
    11281 gcaggaagct tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt
    11341 acgtcaggga actaaaggcc gccagtcgaa aggtaaagca cgtctggcac gctttgaaga
    11401 gctgaacagc accgaatatc agaaacgtaa cgaaaccaac gaactgttta ttccacctgg
    11461 accgcgtctg ggcgataaag tgctggaagt cagcaacctg cgtaaatcct acggtgatcg
    11521 cctgctgatt gatgacctga gcttctcgat cccgaaaggg gcaatcgtcg ggatcatcgg
    11581 tccgaacggc gcgggtaaat cgaccctgtt ccgtatgatc tctggtcagg aacagccgga
    11641 cagcggcacc atcactttag gtgaaacggt aaaactggca tcggttgatc agttccgtga
    11701 ctcaatggat aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg atattatgaa
    11761 gatcggcaac accgagatgc caagccgcgc ctacgttggc cgctttaact ttaaaggggt
    11821 tgatcagggt aaacgcgttg gtgaactttc cggtggtgag cgcggtcgtc tgcatctggc
    11881 gaagctgctg caggttggcg gcaacatgct gctgctcgac gaaccaacca atgacctgga
    11941 tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct gcgcgatggt
    12001 tatctcgcac gaccgttggt tcctcgaccg tatcgccacg cacattctgg attaccagga
    12061 tgaaggtaaa gttgagttct tcgaaggtaa ctttaccgag tacgaagagt acaagaaacg
    12121 cacgctgggc gcagacgcac tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta
    12181 atgcgtaaaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc aagaaatttc
    12241 aatacgttgt gaatctccag gcctgataag acgcggtaag cgtcgcatca ggcattgagc
    12301 accgaatgcc ggatacggcg tgaacgcctt ttccggccta caacagcctt agtatccgca
    12361 aattcgtagc gggtcacccg tgctgattct tttaaacctt gtttaatctc gacagcctct
    12421 tccagagagg ttttcaactc atcaaaaaac ttattttata cctcgccttt gagccgtttc
    12481 atcgcggtta tctctgctcc cccatcatct cccgcaccag ctccacgcag cgcaggaaac
    12541 ggctgtcata atcgtcctct tcaacccgca cgaattcgat attgttctct tccagcatct
    12601 ccaccagcaa gttctggaac tctttgcgat ccaccgaact gccgaggctg cgtaaaccat
    12661 ccgccaccca cggcgtgttg ttctccagca ggatcaccag atcgaaacgg tattcatcaa
    12721 tcaacgcctg tacgaacgga tgctcacgcc cttcgtactt tttgcagaac gcctgggtag
    12781 tgacaaaatc agtgtcaata aacgccactt tattggcata tttcactgca aaatcaatgt
    12841 attgtgcgtg gcccagcgcg attttatcgt aatcggaata ctgcaatgcg atctcatcac
    12901 cgccgaggtg tgaaaagaca taatcgcgac catattccca cgcactggtg gtattgaaga
    12961 tattggcaag tttgtttacc agggtggatt taccgctcga ctcgccacca aggatcgcca
    13021 cggtacgtac aaagaacggc ttcacttccg taggaatata ttcccagtag cggaacgggt
    13081 tttcgcggat ctgcgcaccg ctgatactca taaaggtacg tttcggatcg accagcaccg
    13141 tatcgatccc cagatgttcc atatactgtg gcgcatcggc ttcttccgag gtgtagatca
    13201 ggtccggctg aatccccttt tcagccataa actttttgat gccgttgctc cacacatccc
    13261 agccgtgcgg atacggctcc atgccctctt cgttgaaagc atgaatgcga atatttttct
    13321 gatatttaaa agtttgtaat aaccagcgca gacgatccgg caccgtgggc tgctgcgaca
    13381 tggcgctgtc ttcaaacaac gcgcggtcgc gggtatcgtc aaaacccata atgatatgca
    13441 gctcgtcaac ctggctacag gcgcgctgga taaggtagat atgtccggta tgcagcgggt
    13501 agaacttacc gaatacgaca ccaatggttt tcttctgtcg gggaaattca agccccaaaa
    13561 aacggtgcaa cgcctccagc ttttgcgcgc tggggctttt aattttggca ttcagtaact
    13621 ggcttaaata ccctttggtc ataccgctgg catcagctac ctgctgtagc gtgcagccct
    13681 gttgcttgat ggcagttttc aggtaatcaa atgacgacat atctccctcc gtatctctca
    13741 ttataagtcg tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat
    13801 cccttccggc gcttttttcg gtacgttagc cgccggaaca atcgcccggc gaaaaccgtg
    13861 tttcgccgct tctgaaattc gttcctgacc gctgggcacc gggcggatct cccctgccag
    13921 cccgacttca ccaaacacca ccagatcctg tggcagcggt ctgtcacgca ggctggaaac
    13981 catcgccagc agtaacgcta aatcggcact ggtttcggtt accttcacgc cgccgaccac
    14041 gttcacaaat acatcctgat cggccatttg cagaccaccg tgacggtgca agacagccag
    14101 caggattgcc agacggtttt gttccagccc cactgccacg cggcgcggat tcgccatcat
    14161 cgagtgatcg accagcgcct gaatctccac cagcaacgga cgcgttcctt cccacaccac
    14221 catcacggag ctgccggagg tcacttcatc tccgcgactt aagaaaattg ccgaagggtt
    14281 gctgacttca cgcagcccct gttcggtcat cgcgaagacg cccagctcat tcaccgcgcc
    14341 gaagcggttt ttatggctgc gcaaggtgcg aaaacgggag tcggcatcac catccagaag
    14401 caccgaacag tcgatacagt gttccagcac tttagggcca gccagcgagc catctttggt
    14461 tacgtgccca accatgacaa tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc
    14521 cgccgtttca cgcacctgcg ccacgctacc aggcgatgat tgtacatccg ccatatgcat
    14581 cacctggatc gagtcaatta ccatcagttt cggttgctct tcttcggcaa tcaggcagat
    14641 ctgttcgatg ctggtttccg acaacatatt gagattgtca gtcggcaggc caaggcgatg
    14701 agcgcgcatt gccacctgtt gcagcgactc ttcgccagtg acatacagcg ttttcatctg
    14761 ctgggccagt ttgcacagcg tttgcagtag cagcgtggat ttccccgcac ctgggttacc
    14821 gccaatcaga atggcgcttc ctggcaccac gccgccgcct agtacgcggt cgaactcttt
    14881 aaatccggtg gaaaaacgcg gcagctcttc aaggctgata tcggagagtt tctggacttt
    14941 tgccaccccg gcgctaccgg catagccgct gagacgctcg ttacgcgcca ccgttggcga
    15001 cgcagcaaga cgcacctcgg tgatggtgtt ccaggcatga caggcactgc actgcccctg
    15061 ccagcgcgga taatcggccc cgcattcatt acaaacaaag gcgcgttttg gagcttttgc
    15121 cacggtgtac ctcgttaatg ctgtgccgcc cgcaggatgg tgggcgagca attacttctg
    15181 attcaggctg ccagagagga tgcagaatac ccccatcagg tcagcgtgac ggatggtgac
    15241 ttccgccttt tcattcactt ttggcttggc atggtaggca atccccagcc ctgctgcttt
    15301 gatcatcggc aggtcattag ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc
    15361 atactcctgc gcgaggcgag tcaaagtttt cgctttgtac tgcgcgtcta cgatgtcgcc
    15421 gatcacattg ccggtaaatt taccgtccat gatctccagt tcattggcta ccacggcggt
    15481 caggcgcagc ttgtcgcgca gatattcagc aaagaaagta aagccgccgg aggcaatcgc
    15541 cactttccag cccagcgttt ccagcttgag caccagttgc gttaagcctg gcatcagcgg
    15601 cagattttca cgcacctgtt gcagaatatt ggcgtcagcg cctttcagcg tcgccacacg
    15661 gctgcgcagg ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg ttacttccgc
    15721 caccatctcg cccgttccgg ccagtttggc aatttcatca atacattcaa tctggatggc
    15781 ggtggaatcc atgtccatca ccagcaaacc cggcgtgcgc aggtgcggga ttttccccag
    15841 cggggcgaca tccagttgcg cttcatgggc caggcgcgta gcccgtgcgg tgagtgaacc
    15901 tgccagacga atcacctgat aatcttccac gcaccaggcg gcaacaatca ccatcgccgc
    15961 acccagtttg ctctggtatt gggtcagacg ctgtttatcc agcccacgac catacagcag
    16021 ccagccgcta cgacctgcgt ggtaatccag tggcatcact tcatcaccac ttaatgaaag
    16081 aggcagacca ggccataaag agacatcttc aggcaggtcg caccaggtaa tgttaggcat
    16141 taaggctcct gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta ccttgtatcc
    16201 attgcttctg gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa
    16261 ctgaaattcc ggctgcatcg ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg
    16321 ctgatgcagg gagcgtcatg gtttagtcaa aaccaccagc gacaacgtaa tccacagctg
    16381 gaagaactgg cccgcaccct ggcgcgtcag gtaacgctga acgttgcacc gctgatgcgt
    16441 accgactcac cagatgaaaa acgcattcag gcgatcctcg atcagttaac ggatgaaagc
    16501 cgtatcctcg acgcgggtgt gtatgacgaa caaggcgatc ttatcgcacg ttctggcgaa
    16561 agcgtcgaag tgcgcgaccg gctggcgctc gacggtaaaa aagcgggcgg ctattttaac
    16621 cagcagattg tcgaaccaat tgccgggaaa aacggaccgc tcggctatct gcgcctgacg
    16681 ctcgacaccc atacgctcgc caccgaagcc caacaggtgg ataacaccac taacatttta
    16741 cgcctgatgt tgctgctttc actggcaatc ggcgtagtgc tgacccgcac gctgctacag
    16801 ggtaaacgta cccgctggca gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg
    16861 gaagaggaag aaagcgagaa aaaagagtga cccattacta caagaaagga aatcgttatg
    16921 tccacattac gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtggag
    16981 gagtgtattt ttcgccagat gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc
    17041 gacacggtag tgattggtcg cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg
    17101 gaagaagata acgtccgcct ggcacggcgc agtagcggtg gcggcgcggt gttccacgat
    17161 ctcggcaata cctgctttac ctttatggct ggcaagccgg agtacgataa aaccatctcc
    17221 acgtcgattg tgctcaatgc gctgaacgcg cttggcgtca gcgccgaagc gtccgggcgt
    17281 aacgatctgg tggtgaaaac cgccgaaggc gaccgcaaag tctcaggatc ggcctatcgc
    17341 gaaaccaaag atcgtggctt ccaccacggc accttgctgc tcaatgccga ccttagccgc
    17401 ctggcaaact atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacctcagtg
    17461 cgttcccgcg tgaccaacct caccgagctg ttgccgggga tcacccatga gcaggtttgc
    17521 gaggccataa cagaggcctt tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc
    17581 tccccggaca aaacgccaga cttgccaaac ttcgccgaaa cctttgcccg ccagagtagc
    17641 tgggaatgga acttcggtca ggctccggca ttctcgcatc tgctggatga acgctttacc
    17701 tggggcggcg tggaactgca tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg
    17761 tttaccgaca gcctcaaccc cgcgccgctg gaagccctcg ccggacgact gcaaggctgc
    17821 ctgtaccgcg cagatatgct gcaacaggag tgcgaagcgc tgttggttga cttcccggaa
    17881 caggaaaaag agctacggga gttgtcggcg tggatggcag gggcggtaag gtaactcgct
    17941 gattaaaata tccagggaag gaaatatgat cgatctggaa aaccaggaac gggagattat
    18001 caatttaatg ttcagccagc gtatttcctg gctagccgcc gtgcgtatca ggcataaatt
    18061 atcgcttgct gaagtaagta aaatgttagg tatctcgatt aattctctaa aacagatcga
    18121 aaagacggaa cggcttagct caaacatcaa aagcaaaatg gcagaaattt atggttgtcc
    18181 accagagttg ctgatatgcc cttcctg
//
LOCUS       DAPPNJ010000088        18139 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000088 DAPPNJ010000000
VERSION     DAPPNJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.088"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            77..1474
                     /gene="ulaA"
                     /locus_tag="RPM73_002461"
     CDS             77..1474
                     /gene="ulaA"
                     /locus_tag="RPM73_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDX6520809.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1490..1795
                     /gene="ulaB"
                     /locus_tag="RPM73_002462"
     CDS             1490..1795
                     /gene="ulaB"
                     /locus_tag="RPM73_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDX6520810.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1805..2269
                     /gene="ulaC"
                     /locus_tag="RPM73_002463"
     CDS             1805..2269
                     /gene="ulaC"
                     /locus_tag="RPM73_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDX6520811.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2283..2933
                     /gene="ulaD"
                     /locus_tag="RPM73_002464"
     CDS             2283..2933
                     /gene="ulaD"
                     /locus_tag="RPM73_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDX6520812.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2943..3797
                     /gene="ulaE"
                     /locus_tag="RPM73_002465"
     CDS             2943..3797
                     /gene="ulaE"
                     /locus_tag="RPM73_002465"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDX6520813.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNVIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3797..4483
                     /gene="ulaF"
                     /locus_tag="RPM73_002466"
     CDS             3797..4483
                     /gene="ulaF"
                     /locus_tag="RPM73_002466"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDX6520814.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4612..4887)
                     /locus_tag="RPM73_002467"
     CDS             complement(4612..4887)
                     /locus_tag="RPM73_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6520815.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5214..5609
                     /gene="rpsF"
                     /locus_tag="RPM73_002468"
     CDS             5214..5609
                     /gene="rpsF"
                     /locus_tag="RPM73_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDX6520816.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5616..5930
                     /gene="priB"
                     /locus_tag="RPM73_002469"
     CDS             5616..5930
                     /gene="priB"
                     /locus_tag="RPM73_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDX6520817.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5935..6162
                     /gene="rpsR"
                     /locus_tag="RPM73_002470"
     CDS             5935..6162
                     /gene="rpsR"
                     /locus_tag="RPM73_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDX6520818.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6204..6653
                     /gene="rplI"
                     /locus_tag="RPM73_002471"
     CDS             6204..6653
                     /gene="rplI"
                     /locus_tag="RPM73_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDX6520819.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6765..7736)
                     /locus_tag="RPM73_002472"
     CDS             complement(6765..7736)
                     /locus_tag="RPM73_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDX6520820.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(7789..9090)
                     /locus_tag="RPM73_002473"
     CDS             complement(7789..9090)
                     /locus_tag="RPM73_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDX6520821.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIADIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(9133..9609)
                     /locus_tag="RPM73_002474"
     CDS             complement(9133..9609)
                     /locus_tag="RPM73_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDX6520822.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(9630..11711)
                     /locus_tag="RPM73_002475"
     CDS             complement(9630..11711)
                     /locus_tag="RPM73_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDX6520823.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     LTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(11708..13249)
                     /locus_tag="RPM73_002476"
     CDS             complement(11708..13249)
                     /locus_tag="RPM73_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDX6520824.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(13259..14035)
                     /locus_tag="RPM73_002477"
     CDS             complement(13259..14035)
                     /locus_tag="RPM73_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDX6520825.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(14045..14887)
                     /locus_tag="RPM73_002478"
     CDS             complement(14045..14887)
                     /locus_tag="RPM73_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDX6520826.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASKMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(14897..15688)
                     /locus_tag="RPM73_002479"
     CDS             complement(14897..15688)
                     /locus_tag="RPM73_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDX6520827.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            15901..16548
                     /locus_tag="RPM73_002480"
     CDS             15901..16548
                     /locus_tag="RPM73_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDX6520828.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(16532..17170)
                     /gene="ytfB"
                     /locus_tag="RPM73_002481"
     CDS             complement(16532..17170)
                     /gene="ytfB"
                     /locus_tag="RPM73_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDX6520829.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            17389..18009
                     /gene="fklB"
                     /locus_tag="RPM73_002482"
     CDS             17389..18009
                     /gene="fklB"
                     /locus_tag="RPM73_002482"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX6520830.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         4509 a         4583 c         4618 g         4429 t
ORIGIN      
        1 cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg tggaataccc ggttacctct
       61 tcttcaggag atcgttatgg agatcctcta caacatcttt accgtgtttt ttaaccaggt
      121 catgaccaat gccccgttgt tgctgggtat tgtgacctgt ctgggctaca tcctactgcg
      181 caaaagtgtc agcgttatta ttaaaggcac gattaaaacc ataattggtt tcatgttgtt
      241 gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg gtggtggcga aaatgtccga
      301 agtctacggc attaacggcg caatttccga taattacgcg tcgatgatgg caaccatcga
      361 ccgcatgggc gatgcctata gctgggtagg ttacgcggtg ttgttagcgc tggcgctgaa
      421 catctgttac gtgctgctgc gtcgcattac cggtattcgt accatcatgc tgactggtca
      481 tatcatgttc cagcaggcgg ggctgattgc cgttacgttg tttattttcg gctactccat
      541 gtggacaacc attatctgca cggcgattct ggtttcgctc tactggggca ttacctccaa
      601 catgatgtac aagccgactc aggaagtgac ggacggctgc ggtttctcca tcggtcacca
      661 gcaacagttt gcatcatgga ttgcctataa agttgcgccg ttcctcggca aaaaagagga
      721 aagcgttgaa gacctcaaac tgccaggctg gctgaatatt ttccacgaca acatcgtctc
      781 cacggcgatt gtgatgacca tcttctttgg tgccattctg ctctccttcg gtatcgacac
      841 cgtgcaggcg atggcaggca aagtgaactg gacggtgtac atcctgcaaa ctggtttctc
      901 cttcgcggtg gcgatcttca tcatcactca gggcgtgcgc atgtttgtgg cggaactctc
      961 tgaagcattt aacggtatct cccagcgcct gatccctggt gcggttctgg cgattgactg
     1021 tgcggctatc tatagcttcg cgccgaacgc cgtggtttgg ggctttatgt ggggcaccat
     1081 cggtcagctg attgcggttg gcatcctggt cgcctgcggt tcctcgatcc tgattattcc
     1141 cggctttatc ccgatgttct tctccaacgc caccatcggc gtgttcgcta accacttcgg
     1201 cggctggcgt gcggcgctga agatatgtct ggtgatgggg atgattgaaa tctttggctg
     1261 cgtctgggcg gtgaaactca ccggtatgag cgcctggatg ggcatggcgg actggtcgat
     1321 tctggcaccg ccgatgatgc agggcttctt ctccatcggt atcgccttta tggccgtcat
     1381 cattgtaatt gcactggctt atatgttctt cgctggccgc gcactgcgcg cagaagaaga
     1441 tgcagaaaaa caactggcag aacagtctgc ttaataagga gttttgatta tgaccgtacg
     1501 tattctggct gtgtgtggca acggacaagg cagttccatg atcatgaaga tgaaagtgga
     1561 ccagttttta acccaatcaa acattgacca cacggtaaat agctgcgcgg ttggcgaata
     1621 caaaagcgag ttgagcggcg cggatatcat catcgcttct acgcacattg cgggcgaaat
     1681 caccgtgacc ggcaacaaat acgtggttgg cgtgcgcaac atgctctctc ctgccgactt
     1741 tggcccgaaa ctgctggaag tgatcaaagc gcatttcccg caggatgtga agtaaggacg
     1801 cgccatgaaa ttacgtgatt cgctggcgga gaataaatcc atccgcctgc aggctgaagc
     1861 tgagacatgg caggacgcag taaaaatcgg cgttgacctg ctggtggcgg cggatgttgt
     1921 cgagccgcgt tactaccagg cgattctgga tgcggttgaa cagcacggtc cgtactttgt
     1981 gctcgcacca ggcctggcaa tgccgcacgg tcgcccggaa gagggcgtta agaaaaccgg
     2041 ttttgcgctg gtgacgctga aaaagccgct ggagttcaac cacgaagata acgacccggt
     2101 ggatatcctc atcaccatgg cggcggtcga tgccaacacg caccaggaag tgggcatcat
     2161 gcagatcgtc aacctgtttg aagatgaaga aaatttcgac cgtttacgtg cctgccgtac
     2221 cgagcaggaa gtactggatc tcattgaccg caccaacgcg gcagcttaag aaggaattga
     2281 acatgtcatt accgatgttg caagtcgcgc tggacaacca gacaatggat agcgcctacg
     2341 aaaccactcg cctgattgcc gaagaagtcg acattatcga agtgggtacc attctgtgcg
     2401 tgggcgaagg cgtgcgtgcg gttcgtgacc tgaaagcgct ctacccgcac aaaatcgtac
     2461 tggcagacgc taaaattgct gatgcgggca aaatcctttc gcgtatgtgc ttcgaagcca
     2521 acgctgactg ggtgacggta atttgctgtg cggatatcaa caccgccaaa ggcgcgctgg
     2581 acgtagcaaa agagtttaac ggcgacgtgc agatcgaatt gaccggctac tggacctggg
     2641 aacaggcgca gcagtggcgt gacgcaggca ttcagcaggt ggtttatcac cgcagccgtg
     2701 acgcgcaggc cgcaggcgta gcgtggggcg aagcggacat taccgcgatc aaacgtcttt
     2761 ccgatatggg cttcaaagtc accgtcaccg gtggcctggc gctggaagat ctgccgctgt
     2821 tcaaaggtat tccgattcat gtgtttatcg ccggtcgcag catccgtgat gccgcttctc
     2881 cggttgaagc cgcgcgtcag ttcaaacgtt ccatcgccga actgtggggc taaggagcgg
     2941 atatgttgtc caaacaaatc ccgcttggca tctatgaaaa agcgctcccc gccggggagt
     3001 gctggctgga acgcctgcga ctggcaaaaa cgttgggctt cgattttgtc gagatgtcgg
     3061 tagatgaaac tgacgagcgc ctgtcgcgcc tcgactggag ccgcgagcag cgtctggcgc
     3121 tggtgaatgt gattgttgaa accggcgtgc gcgtgccgtc catgtgcctt tctgctcatc
     3181 gtcgtttccc gctgggcagt gaagatgacg cggtgcgggc gcaggggctg gagattatgc
     3241 gtaaagctat ccagttcgcc caggacgtcg gcattcgcgt gatccagctg gcgggctatg
     3301 acgtttacta tcaggaagcc aataacgaaa cgcgtcgtcg tttccgtgac ggcctgaaag
     3361 agagcgttga gatggcgagc cgcgcgcagg tgacgctggc gatggagatc atggattatc
     3421 cgttgatgaa ctccatcagc aaggcgctgg gttacgcgca ctatctcaac aacccgtggt
     3481 tccagctcta cccggatatc ggcaacctgt cggcgtggga caacgatgtg cagatggagt
     3541 tgcaggccgg aatcgggcat atcgtcgcgg tacatgtgaa agacaccaaa cctggcgtct
     3601 tcaaaaacgt gccgtttggc gaaggtgtag tggatttcga acgttgtttc gaaacgctca
     3661 aacagagtgg ctattgcggg ccgtacctga ttgagatgtg gagcgaaacg gcggaagacc
     3721 cggcggcaga agtggcgaaa gcgcgtgatt gggtgaaagc gcgcatggcg aaagcgggca
     3781 tggtggaggc ggcataatgc aaaagctaaa acagcaggta tttgaagcca acatggatct
     3841 gccgcgctac gggctggtga cctttacctg gggcaacgtc agcgctatcg accgcgaacg
     3901 cgggctggtg gtgatcaagc ccagcggcgt cgcctacgaa accatgaaag cagacgatat
     3961 ggtggtggtc gatatgagcg gtaaggtggt ggaaggggag tatcgcccat cttccgacac
     4021 cgcgacgcat cttgaactct accgtcgtta tccgtcgctt ggcggcattg tccataccca
     4081 ctccactcat gctaccgcat gggcgcaggc ggggctggcg atcccggcgt taggcaccac
     4141 gcacgctgac tacttctttg gtgacatccc gtgtacgcgt ggattaagcg aagaagaggt
     4201 gcagggtgag tatgaactga acactggcaa agtgattatc gaaacgctgg gcaacgccga
     4261 gccgctgcat acgccgggaa ttgtggtgta tcagcacgga ccgttcgcct gggggaaaga
     4321 tgcccacgac gcggtgcata acgcggtagt gatggaagaa gtggcgaaaa tggcgtggat
     4381 tgcgcgcagt attaatccac aactcaatca catcgacagc ttcctgatga ataaacactt
     4441 catgcgtaag catggtccta acgcttatta cgggcagaag taaaacacgc gctgcggaaa
     4501 tttccttctt cgggagataa ctggtctaat tccgcagccg tttttcaaaa aaagccccct
     4561 gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt gctagcgata
     4621 aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca gaattacgtg
     4681 gtagtacgtt gcgccctgcg catctgtttc ttcatttaat gcctgacgtg cttcactttc
     4741 agtggcgaaa ttatgattga tataaatcac gcctaagctt tgcacatcgt ccatatttct
     4801 ggcctggtgg ttattgattt caatggctgc ccatgtattt gcacttagca aaagcacagc
     4861 cagaagggct aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc
     4921 tcctctgtga tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca
     4981 tttctgatgc agttgttcaa aaaaacacca tgatgaagtg tgatgaactc caaatcagcg
     5041 gtttagaggt taattgcgaa aggggagatt tatttcggct ctgcccttga gtttagcggg
     5101 gcatacaagt actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc
     5161 cgggattcgg ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc
     5221 attacgaaat cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg
     5281 agcgctacac tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg
     5341 gccgccgtca gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga
     5401 acgttgaagc tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg
     5461 ccgttatccg cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg
     5521 ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg
     5581 atgctgaagc tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg
     5641 gcaccgtgtg caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt
     5701 tcgtgcttga gcatcgttct gtgcaggagg aagccggctt tcaccggcag gcgtggtgcc
     5761 aaatgcccgt tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg
     5821 gcagtcgcat aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca
     5881 aaatggtttt gcatgccgag cagattgaat tgatagattc tggagactag ccatatggca
     5941 cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac
     6001 tataaagata tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc
     6061 cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct
     6121 cgctacctgt ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa
     6181 tacgactttg agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg
     6241 gtagcctggg tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc
     6301 agggtaaagc tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg
     6361 aactggaagc taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca
     6421 atgcactgga aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt
     6481 ccatcggtac tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga
     6541 gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg cgaacacgaa gtgagcttcc
     6601 aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta
     6661 ttcaacgaga cgtaaaaacg ccgaccattg gtcggcgttt tgcttttgta gcgagcactt
     6721 gcagatatcg ataagggcca caataagcag ccctacgaag ataattactt agccgcctga
     6781 atatcagcca gcattggggc aagggctggg ttatccttag cgaagtttgc gtacattggc
     6841 tgtactttct cgataaacgg agctttatct acctggtaga ctttcacgcc cattttatcc
     6901 agttttgcaa attcttgttt ctcttcctcg ctccacaagt ctttcatgta gctcattgat
     6961 tctttagctg cttttaatat aactgcctga ttttccgggc taagtttatc aaacgcagca
     7021 ttgctcatca gaaccacatc gggaaccata gtgtgttcgt caaggctgaa ggattttgcc
     7081 acttcaccgt gacgcatggt ggtcagcgcc atcacgctgt tttctgccat atcgaccaca
     7141 ccctgctgta aggctgtata caactcgccg taatccagtg gcgtcgggtt accgcccatg
     7201 actttgatca tttcaactgc gccagggctt ggctggacac ggactttcat gcctttcaga
     7261 tcgtctggtt tcagtactgg tttattgcca taaaaactgc gggcgcctcc atcataaaaa
     7321 gtcagcccga aataaccttt gcttttcgtt gagtcgagga tttttctccc gatatcgccc
     7381 tgcatgacct gataataatg atcacgatcg cggaacaaat aaggcaggct aaataattta
     7441 taggaatttt caaatgcttc catttctgcc gcattggttt ttaccagtgg aatagcgcca
     7501 gaacgaatca gctccattgt ttctcgttga gtacccaatg ttccatttgg ataaatacga
     7561 atagtaatat caccgttaga gtactctttg ctctttttcg caaagaactc catcgcttta
     7621 tgaacaggat gagacttatc ctgattatga ctcagtttta atgtcactgc ccaggactgg
     7681 cttgcggata acataagaac agcaccgata atgccagcgg atacttttat tttcatagat
     7741 gttccttttc taataaatat ggcggcaggt gccgccagtg aatgcgcatc agtagcccag
     7801 tgctcttggc aatgccatac tgatttgcgg aatataggtc accattgcca gcaccaatac
     7861 catcacggca tagaatggca gcataggttt gataatttta ttaatcggta atttagatac
     7921 actacaaccg acaaataaca cactgcctac aggcggagta caaataccga tacacatgtt
     7981 cagcaccatg acaataccaa agtgaatcgg gtccatgccc agttcggtga caaccggtaa
     8041 aaatatcggc gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa
     8101 caggataata ttgataataa gcaggattat tattgggttg tcagaaatag tgttaagcaa
     8161 atcgagaatc aggaagggaa cgtcagcgtt tgacattgcc catgacattc ccatcgaaga
     8221 accaattaat aacaagacaa ttgcagtggt aataacggat tccagaaata ttttaggtaa
     8281 atctttaact gagatttcac gataaagaac cagtgcaagg aaaagggtat aaattaccgc
     8341 aattgcggag gcttcggttg gagtaaaaat accgcttagt acgccaccca taattaacac
     8401 aatcaggaaa atactgggga tagccatcca tatcgcaacg ccagcctggc gcaaagtcgg
     8461 acgttctggt tttggataac cgcgacgtac agcgataata tcggctatta ccatcagtgc
     8521 cagacccagc aaaatccccg gaacataacc cgccaggaac agtgcggcaa tagaagtacc
     8581 accactgacc agtgaataga cgattaacgt attacttggt ggaataagca aacccgtcgg
     8641 ggcagaggca acgttaaccg ctgtactgaa tgccgggtcg tagccctctt taacctgttg
     8701 cggatgcatg acgccgccca tcgcagccgc cgaagccacg gctgaaccag aaattgcccc
     8761 gaacagcata ttggcgagaa tattacagtg ggcaagtgag cctggcagac gaccgccgag
     8821 aatacgggca aagttgatta atcgtcgcgc tattcctcca tgattcataa tgttgccaga
     8881 caagataaaa aatgggattg ccaacaacgt aaaactatct aacccggagg ctaacttttg
     8941 ggaaaccaca ctcaatgctg agttgaaatc gagttgtgtg gaaattgatg ccagtgtcga
     9001 taaaccaatg gcataaacaa taggtacacc taatgccaac ataaaaaaga aggcaccaaa
     9061 taacacaata atcggtaacc agtaatccat aatttctcca ttcctactat ggttggttgt
     9121 tgtttgttat ctttagagtg gttgtttaag ttcggttaaa accatatcta tggtgtatat
     9181 aataataagt acaccactaa tcgggatact catatatata tatcccatct gccagcctaa
     9241 agcgggcgag gtttgacctg aatcgaatgt atccatcgtt aatagcccac caccatatac
     9301 cataatgatg gtagcgaaga taattacgat aatctgaata cacaaaaata aatgacgtcg
     9361 ggcttttcct ttcagcatca taggtagcag gtcaatcgcc agatgccggt tttgccctgc
     9421 ggtgtatgcc ccaccaaaaa gtccaagaca aataaagagc aaacggctga gttcatctgt
     9481 aaaaatactg ggactagtaa atataaatcg tgcagccacc tgccacgtca cacatgccac
     9541 gatgatcgcc aaaatggtaa tgttcaatgc tgcaagcaga gcattaatag atctaattat
     9601 atttcgcata gtgattaacc tttgcaatat cattcatcct gtggctggta aattgtccca
     9661 ttaagaaaac caggccagtt tttttcaaac caaatacctc ggataatagg aaactcacga
     9721 acaggtgttt ctctgacgtg aaagataccg ttgcaataat gaataagtag tttatctttt
     9781 ttatcctcag tacgctctga acgaaagcgc caggcatcac aaaaaccatt tcgtgttagc
     9841 ggaatagaat ttccatccct gtcgagaata atacgtgctc cccggctacc accacctgcg
     9901 tcaatataat gcgttaattg cgttaatacg gcggcagaag tcagtgccat atgccgccac
     9961 ataaacagct cggcaacttc acccacatgt ttgatagcca atccatgccg ttggacaaat
    10021 tcgttcaata acagggcatc acgagtggca cgtcggactt tatcggcatg gcaaataaat
    10081 cccgcatggt cagacattcg tgcctgaatt ttttctctca caaccgacaa cggcatcccg
    10141 gtcccgttat cgtgcgcctg agtaatgatc tctcttatag aagccagtgc cggagctacg
    10201 agctgtgcta tatctccatc aatattccgc ttttgcgtgc aaccaataaa acgtgccaga
    10261 cgaacagcaa aaacctgccc ggcattcagc gccgcaccgc ctggtcgggt aacgccgtga
    10321 gttccggcta cttctcccac cgcaaaacaa ccgggcaggg atgtttgtcc ccagatatcc
    10381 acttcaatgc cgccattcat atgctgatta ttcatggcaa actgcaaagg ctgtgtggtg
    10441 agatcgtaac cgtgcatctt atacagcgag atagacagcg gattcattcg ttgcagccgt
    10501 tcgatgggcg atggtgccag agcgtcgtta ttttccagat acgcgcgaac gtcgtcgtcc
    10561 agtcgatcta atgagaaggg caaatcacca ggcaccgctt caggattgcg attgaaatcc
    10621 ataaacaccc gacgaccatg ttgctgctct tgcgctactg ccatatctaa caagctggag
    10681 ccaaaatcca tcacccgagt ggcgtggaac ggccactgat agcctttacg gaaaatgttt
    10741 gaagccagct cctgggtggt gcgatagtaa tccgcgagga agttatactc gttaccctca
    10801 gcatccacgg aatagatata cgggatcacc tgtacatagg tgccggataa attccacgga
    10861 aacgtgctac gcggcgtgcc tataccaaac tggctttcgg tcagattggt tagcgtcaaa
    10921 ccttcctcca gcgccagccc cagcgagcca aaacatttgt gtgggtacac actgtcgcga
    10981 taaagctcgc caggccctcc tgttgccagt accacattgg gcgcagtcac aattgccagc
    11041 ccccatgggt tatgggcgcg atgaccggtc gcgaggattg cccccgccac acggtcttcg
    11101 ccattttcgt cacgctgatg cagcagttta atcactgttg cactggtcaa cactggaatg
    11161 gcgaggcgct gtacttcttc caacagcact ttcaccatta accttgaggt acgcggccca
    11221 cacgaggttg cacgcccggc ttcgtcatgg tcggtttgat aacgaagaat cgcgccatag
    11281 cgatcttccg gtaattccag accaagatat tgcaacccgc caagagtgtg cagagaacct
    11341 accgcttcga cataagcggt gtcgtgatcc atcgccccac cgctcgccag tgcctctgcc
    11401 agtttggtga agttgtcgcc attgcctgcg gtagcggcgg taaacagcgt ctgtttatcg
    11461 gaaccagaac atgccgacgt ccccatatat aacccggcgg tggcgatcag cacattttgc
    11521 tggcggcgtt taagttccac agctgcacgc agcccggctg caccactgcc gactaccagg
    11581 gcatctgttt gccagagtgg gtaatcaaca ccgtctaccg tgacaaccca tgcgggctct
    11641 gcctgtgcaa aaccctttgg gaaagtaacg tgcgtcagcg catcaaggat ggtgtcatca
    11701 agctgactca tgcatcctcc atcggcgtaa acaggctact ttccatcagg cgtgggtgct
    11761 tgataattgg acggaaagcc atatacggca gaatgtcgcg ctcaatctcc acacccggcg
    11821 cgatttcaat taattcaacg ccttctgccg ttaactggaa tactgcgcgt tcagtgatat
    11881 acaacacttc ctgaccactt tgctgtgcgt aggcggcgct aaaagtgatt ttttccacct
    11941 gggtaaccag tttagggatt tgacctgact gggtgatatg caagccatct ggcgttacat
    12001 caatcttgct acctttggcg tcgaacgtgc cgcagaacac cactttacgg gcgttttgcg
    12061 caatttcgag aaatccgccg gggccaatca gattgccatt gagatgggag acgttgacgt
    12121 taccgtactg atccatctct cccatgccga ggaaggtgat atcgatccct ccgccactgt
    12181 agaactcaaa ttgatcgagt gatggaataa tggcatcggc gttgcgggca caaccaaaca
    12241 gagcatcatc aagcatcatg ccgttatgta cgccttgctc gacgctcagc cataaacttt
    12301 ggtaaggaac gccttcgcgc agcgctacgc ctggaatacc gccaggaata ccaaagccaa
    12361 agttggttga agcgccagcc accagttcgc gtgctgcccg acgggcaatc aggcggcgca
    12421 ccggatgact aaccagttca atagcgtcgt tagaactcag acggcgatgt tgaccgctaa
    12481 tggtcaggtc gtaaccgcca agataggttt gcggttgttc gcggtgttca acgatgccat
    12541 caaccagaat gcccggtaat ttgacccggc gtggttcaat cgcgccagct tccagcacat
    12601 cgcgtacctg tacgaacact ttgccgccgc tgttgtgtgc tgccagcgcc atggaatagc
    12661 tatccatatc aatcgcttct tcttcaaggc tgacgttgcc tcgaggatcg gcataagtgc
    12721 cacgcaaaat cgcgtaatcc actttgaaag ggcgataacg aagtttggtt tcaccatcga
    12781 tttcaatcag ttctaccagg tcgtcggtag tgcattcatt cgacttaccg ccgccattgc
    12841 gtggatcaac aaacgatccc agcccaacgt gggtaaaaag ccccggacgt ccggcgccga
    12901 tctcccgtag cagtgcctga atcacgccac caggaaagca gtaggcttca atcgtgttat
    12961 tttttaccag cgcctgcatt ttgggcgacc aggtaaagtg cccggcaata atgcgtttga
    13021 gcatctctgc gtgcgcaaaa cgattagtgc ctttgcagtc gcgatcgcca atacccagcg
    13081 agtggattag cgtcaggtcg cgagggtgtc cggtttgcag gaaacgcgct tcaatagcag
    13141 ccagaatatg gtccgcttcc accataccgc cgccgttacc gctaatagca atagtcgcac
    13201 cgtcctgaat ttgcgccgcc agtgcctcgg cggttgttat cttacgcata caatttcctt
    13261 attgattatg gaagcttacg ctactttttt cggcaaaggc gcgcgtcgcc tgctggcaat
    13321 cctgagtggc ggacattaat ccggccagta gctggtgcgg gtaatcggca tgtttatttt
    13381 ccagtgccag aatcagttgc ttaatatgtg caagcgccac cggaccgcat ttttccagct
    13441 gcgctaactg gttcgccatc cagctttcca cctgttcttt ttctaccact tcatcaatta
    13501 aattcgccgc ctgggcttcc tgcgccgtca ggcgtttacc cagcatcagc atctggcgac
    13561 cgaccaccgg accaacctga ttaagcacac gttcaatgcc catccagcca ggcaccatgc
    13621 cgagcatcac ttccgggtta gagaatttcg cgccggggcg ggcaatacga atatcgcagc
    13681 acaaggcaag ttcaatacca ccgccgatgg tatgaccgtt gagattcgca acggttaatt
    13741 ggggtaagtt gcgcaggcga ttaaaaacgt cattaccgcg tttaatccat tttctgccca
    13801 tatctaatgg cgaataggct gaccacgatt taatatcgcc acccgcacaa aagaatttat
    13861 cgccggttgc cgttaattcg actaatcgta atgtggtgtc gttttcaatt tcatttaaat
    13921 aatggtcgag agattcgatc atctcttcgt taatagcatg acatttatct tcacggttca
    13981 atgtcaggcg gcaggaagct gccgctctgc tgaacagaac gggttggtca ctcatgatca
    14041 ttccttaaca ataatgcgcg atgcgatcga cgctaatatc cagcgtgctg ttaatatctt
    14101 tctgccacca gtaaggcgag aatatttcca gttcaatagc accattaaat ccggcgtttt
    14161 caaccagccg acgaattgac ggaatattga tgacgccatc tcccggcata ccacggtcat
    14221 tgaccagatc agtggtcggc actaaccagt cggaaacatg aaacgcgagg atgcgttttc
    14281 cggcacgcag gatctgactg gcgagatccg ggtcccacca aacatggtag acatctaccg
    14341 ccacgccgag gccaaattcg ccatcgggat cgagttcatc acaccagtcc agcgcctgac
    14401 gtaacgtgca cagacaggaa cgatcggctg cggtcatcgg gtgcagcggc tccagtgcaa
    14461 ttggcacgcc gacatctttc gaatgtggca gcaactggcg aatcccttgt ttcacttgtt
    14521 cccgcgcttc atatagatct ttagtgcccg ttggcagacc gccaaccacc tgcatataac
    14581 aggcggcatt cagtacagcg gcgtcgtcca gcgcccggcg gttatcgtca atcgccagtt
    14641 tgcgctccgc cagcgtcggt gcggtgtagt aggtgctacg gcacaaaccg gaaacgttca
    14701 tcttactggc ggcaagctga cgggcgatct gctggagatc ttcgccctgc aactccctgc
    14761 gccagggggc aattgcgcca ataccgcgtg cagcgcaggc atcaataatg gcgggtagcg
    14821 gcgttttata gcccagtgtt gcggtattga tactgaataa atccgggcgt tgtaacagat
    14881 ctctcatgat tcagcctcag aagcgcggca tacttaagcc gccgtcgatg gcgaccgcct
    14941 gaccacaggt gtaaatcagt ttaccttccg ccatcgcgcg gacggtggaa gcaatatcag
    15001 ccggataacc ccaacgtccc cacggcacca gacctttggc aatcagttcg tcgtaataag
    15061 ccgttgccgg aatagtcata tcagttttga tgagacctgg gcgcacttca taaacgccaa
    15121 tttgttcatt acaaaggcga gcagcaaata gacgagcagc ggcagaaacg gcggttttgg
    15181 caatggtgta ttcgccgcga ttcatcgcca gcatgatggc gttgatggaa ctgacgaaaa
    15241 taatcgagcg gtgcggcagt tccgcttccg gtttcggctg tgccagtaag cgcttactga
    15301 atgcctgagc aaggaagaac ggcgcgcggg tgttaatggc aatgttttga tcgaagctgt
    15361 ctggttcgag gtcgaggagg tcaccgcgtt ttttcactga aatcccggcg ttattgagca
    15421 ggcagtccag acgcccccac tggttttgcg ccgcgtcgag catctcttca tgcaaggaga
    15481 gatcgccgac atcggcaggg aagcaaatga cttccacgcc ttctgccaaa cattcttgtt
    15541 gtgtgatatg cagtttttct acgctgtcgg catcgggacg atcgttaatc agcaggttaa
    15601 acccgccacg agcaagctcc agcgcacagc cctttccaat accacgagcg gctccggtaa
    15661 tcacggcaac aggacgagtg gatttcatac aatagctctc tttttttgaa tattgttttc
    15721 taagtgacaa ttagcttgcg acgtgccgca agaaagatca accatctggt tggttgatat
    15781 gcgatagtga tcacaggaca tttgataact tgatgaaagt acaagttttt atccttttta
    15841 ctggagcttc taattaatcg ggttaagata gttatcttga agaggtcgtg gggaagcgtt
    15901 atgtatcttg ataccagcaa cgtacagagc ctgaaggaaa agctgctgtt atgcgcggtt
    15961 aatgaatttg ccgaatatgg ttatgaaggg gccagggtcg ataatattgt taaggctgcg
    16021 gggtgtagta aacagacggt ttatcatcac tttggtaata aagaaaattt atttattgaa
    16081 gtactggaat atacctggaa cgatattcga caaaaagaaa aggcccttga tttctcagat
    16141 cttccgccac aaaaagctat tgagaaaatt attgatttta catgggatta ttatattgct
    16201 aacccctggt ttttaaaaat tgtgcatagt gaaaatcaaa gtaaaggtgt gcattatgca
    16261 aaatcacagc gtctcttaga aattaaccat gcgcacttgc agttaatgga atccttattg
    16321 gatgaaggga agaaacacaa tatttttaag ccagatatcg acccattgca ggtgaatatt
    16381 aatattgctg cgcttggggg atattatttg atcaaccagc atacgcttgg cctggtttat
    16441 catatcagta tggtttcccc ccaggcactg gaagccagac gtaaggtcat caaagagaca
    16501 atccttagct ggcttttggt tgacccttca tctaccgcgc acgaataaaa ctgccatccg
    16561 gctggcgggt aaacaggacc tgttgattat tccccgtatc aatggttaag cccgtcacca
    16621 cgccgctggc gttctggcgg attttcacca tttgcccgtt ttgcaaatta ctcagcggct
    16681 tacccgcgcc ttctacctgc gccattgcat agacatcggt agcgggcagt ccgtggtcgc
    16741 ggaacagctg cgccatcgtt ttaccgggtt cgacacggta agagcgccat tgattatcaa
    16801 ttccgctatc tggctggaat ggttgcgttt gggtagtttg cggctgctct tctggctggc
    16861 cttcctgtat aggttctgga gcgaccggag ccacctgatc aggatcattt tgtggggtga
    16921 ctaattgcgc ccgcagctgt tcttctgtcg gcggttgtga ttgcgactga atatctaact
    16981 gcgcttcacg cgtcaccacc ggcgcgttgg gcgtatcatc agatggaagc agaaatccga
    17041 ccaccaatac aatggcggca ataatgatcc cccgacgatg catcggcggc agcgggtcca
    17101 tgaagcgaaa attgtccggc gcgtgccaga ctttctccag ggttggtttt agttcaaagc
    17161 gcccgggcat ggtttcctcc tgctccgcgt cttgttcctc aatcatagcc tatgaataag
    17221 ctaacgctat gatgtccgtg gtaaacccgc ctttattata ttcatacggg atattgctat
    17281 tgtttctttt tccccgggat ttgtcatcat tcctgcgaca aagttttacc caaagaagtg
    17341 tggctgatat gctgcccgct acttttatac cctaagaaag gaaatacgat gaccacccca
    17401 acttttgaca ccatcgaagc gcaagcaagc tacggcattg gtttgcaggt agggcaacaa
    17461 ctgagtgaat ctggcctgga agggctgctg ccagaagcac tggttgcagg tattgccgat
    17521 gcgctggaag gcaaacatcc ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa
    17581 atccacgagc gcgccgatgc cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt
    17641 gtgaaatacc tggaagaaaa cgccaaaaaa gaaggtgtga atagcaccga atctggcctg
    17701 caattccgcg tgatcaacca gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt
    17761 gttcattaca ccggtaaact gatcgacggc accgtgtttg acagctccgt tgctcgtggt
    17821 gaacccgctg aatttccggt taatggcgtg attcctggct ggattgaagc actgactctg
    17881 atgccggtag gttctaaatg ggaactgact atcccgcagg aactggcata tggcgagcgc
    17941 ggcgcaggcg catccatccc tccgttcagt accctggtgt ttgaagtcga actgctggaa
    18001 attctctaag cagcgcattc tgttcccctc gaaccagagg ggagcaggca ttcagcaata
    18061 aacccttcag tttgccaaac ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt
    18121 tttgtgagct gtttcgcgt
//
LOCUS       DAPPNJ010000089        17823 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000089 DAPPNJ010000000
VERSION     DAPPNJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.089"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            138..863
                     /locus_tag="RPM73_002483"
     CDS             138..863
                     /locus_tag="RPM73_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX6520831.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            821..1498
                     /gene="bglJ"
                     /locus_tag="RPM73_002484"
     CDS             821..1498
                     /gene="bglJ"
                     /locus_tag="RPM73_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDX6520832.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1536..2324)
                     /gene="fhuF"
                     /locus_tag="RPM73_002485"
     CDS             complement(1536..2324)
                     /gene="fhuF"
                     /locus_tag="RPM73_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDX6520833.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLSTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2465..2701
                     /locus_tag="RPM73_002486"
     CDS             2465..2701
                     /locus_tag="RPM73_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDX6520834.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLLSCLALIAAIMLMIMNLNQG"
     gene            complement(2740..2826)
                     /locus_tag="RPM73_002487"
     tRNA            complement(2740..2826)
                     /locus_tag="RPM73_002487"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2790..2792),aa:Leu,seq:cag)
     gene            complement(3094..4125)
                     /gene="rsmC"
                     /locus_tag="RPM73_002488"
     CDS             complement(3094..4125)
                     /gene="rsmC"
                     /locus_tag="RPM73_002488"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDX6520835.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4228..4641
                     /gene="holD"
                     /locus_tag="RPM73_002489"
     CDS             4228..4641
                     /gene="holD"
                     /locus_tag="RPM73_002489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDX6520836.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4610..5056
                     /gene="rimI"
                     /locus_tag="RPM73_002490"
     CDS             4610..5056
                     /gene="rimI"
                     /locus_tag="RPM73_002490"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDX6520837.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5071..5748
                     /gene="yjjG"
                     /locus_tag="RPM73_002491"
     CDS             5071..5748
                     /gene="yjjG"
                     /locus_tag="RPM73_002491"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDX6520838.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5839..7428
                     /gene="prfC"
                     /locus_tag="RPM73_002492"
     CDS             5839..7428
                     /gene="prfC"
                     /locus_tag="RPM73_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDX6520839.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7821..8426
                     /gene="osmY"
                     /locus_tag="RPM73_002493"
     CDS             7821..8426
                     /gene="osmY"
                     /locus_tag="RPM73_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDX6520840.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8553..8714
                     /locus_tag="RPM73_002494"
     CDS             8553..8714
                     /locus_tag="RPM73_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDX6520841.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8836..9909
                     /locus_tag="RPM73_002495"
     CDS             8836..9909
                     /locus_tag="RPM73_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDX6520842.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9906..10688
                     /locus_tag="RPM73_002496"
     CDS             9906..10688
                     /locus_tag="RPM73_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX6520843.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(10801..11664)
                     /locus_tag="RPM73_002497"
     CDS             complement(10801..11664)
                     /locus_tag="RPM73_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDX6520844.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11636..13186)
                     /locus_tag="RPM73_002498"
     CDS             complement(11636..13186)
                     /locus_tag="RPM73_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDX6520845.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13444..14223
                     /gene="deoC"
                     /locus_tag="RPM73_002499"
     CDS             13444..14223
                     /gene="deoC"
                     /locus_tag="RPM73_002499"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDX6520846.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14350..15672
                     /gene="deoA"
                     /locus_tag="RPM73_002500"
     CDS             14350..15672
                     /gene="deoA"
                     /locus_tag="RPM73_002500"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDX6520847.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15724..16947
                     /gene="deoB"
                     /locus_tag="RPM73_002501"
     CDS             15724..16947
                     /gene="deoB"
                     /locus_tag="RPM73_002501"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDX6520848.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17027..17746
                     /gene="deoD"
                     /locus_tag="RPM73_002502"
     CDS             17027..17746
                     /gene="deoD"
                     /locus_tag="RPM73_002502"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDX6520849.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4306 a         4524 c         4787 g         4206 t
ORIGIN      
        1 cagcaggagt atgctgatat gaaagtgaat gctaaggata atttattcgc ttaatctatt
       61 aatttgctgg aaacatttaa ggaatgaata tcctgaataa accatcatga tttttagaca
      121 gtggatgtgg aggaaatatg ttgccaggat gctgcaaaaa tggaattgtt atcagtaaaa
      181 tacctgttat gcaagcaggg ttaaaagagg tcatgaggac tcacttccct gaatatgaaa
      241 taatatccag cgcctctgcg gaggacctta ccttattaca attacgtcgt tccggattag
      301 tcattgctga tttagccggt gaaagtgaag atcctcgttc tgtttgtgaa cattattatt
      361 ctttaatctc acaatatcgg gaaattcact gggttttcat ggtgtcacgc tcctggtatt
      421 cccaggcagt agaattgctc atgtgcccta cggcgacgtt attgtctgat gttgaaccca
      481 ttgagaatct ggtcaagacc gtacgttccg gcaatacgca cgcagagcgt atcagcgcca
      541 tgctgacctc tccggcaatg aatgaaactc atgattttag ctatcgctcc gtcattctca
      601 ctctttcaga gcgcaaggta ctgcggctat taggtaaagg atggggcatc aaccagatag
      661 cttcgttgct taagaaaagt aataaaacta tcagcgccca aaaaaacagt gcgatgcgtc
      721 gactggcaat tcacagcaac gctgaaatgt atgcatggat aaatagcgcg cagggtgcaa
      781 gagaacttaa cttgccttct gtttatggag atgccgcaga atggaacaca gccgaattaa
      841 gaagagaaat gtcgcactca tagaaaaatg cgtcatgagc agtatcggta ttgagagttt
      901 attcagaaag tttgcgggta acccttataa gctccatacc tataccagtc aggagtcatt
      961 tcaggatgcc atgtcgcgga tctcatttgc ggcggtcatt ttttcttttt ctgccatgag
     1021 aagtgagcgc agagagggat tatcttgcct gactgaactg gcgattaagt ttccgcgtac
     1081 ccggcgttta gttattgcgg atgatgatat tgaagctcgg ctgattggtt cattgtcgcc
     1141 atcaccgctg gacggtgtat taagtaaagc gtcaacgctg gagatttttc atcaggaact
     1201 ctttttgtca ttaaatggtg tacgtcaggc gaccgaccga ctgaacaatc agtggtacat
     1261 taaccaaagc cggacgctaa gcccgacgga gagagaaata ttgcgcttta tgtcgcgtgg
     1321 ctactcaatg acacaaattg ccgagcagct taaacgcaat atcaaaacga tccgtgcaca
     1381 taaatttaat gtgatgtcga aactgggcgt cagttctgat gcagggttgt tggaggccgc
     1441 agatattctg ttatgtatgc ggcattgcga agcaagtaat gtgttgcatc cctattaatc
     1501 tgcatgatgc cgggtttact tccccggcag tgctttcatt tcagcgtaca atcgccacat
     1561 tgctgcacat ccggtaagcg ataacgctgg cagcaggtcc ggcgcaccag caggccgtcg
     1621 cgtagcacca cggtacgcca gagtggatta tcttcaccgg tagaaagcgt tttctcaaaa
     1681 aagagggcat ggcgcagcga ttcaaccgtc gcctcgccga gcagttgctt catctcagtg
     1741 agataccagt tgatcaaata accggtatta ctccagataa gtttgccgtt gatctctccg
     1801 gtcgcttcta gtgcctgcac aaccggaacc agcgcctggc tgattaacgt ttccattcgc
     1861 tgctgcggcg aatgtggtgt tgcgttttta tcttcacaca catcgaccca gaaacaggcg
     1921 gcacggccgg tttcgtgaaa ctcagcatgg aaatgttccg gcgacacatc taatgccttt
     1981 tcctgcgtca gtagcgccag cattaatggt ggcaccatca ggccgatata ccattgtgcc
     2041 catagcgaga tcagcggttt gttctcgcgg atcatcgttg gttggttgcg atagatatga
     2101 tcggaataga ccgcgagtag agaacttagc gcattcggtg atgaccattg cgccagcgtc
     2161 atggcgttaa gcggagcggg ttcatccagg cggataaact ccagcaaatg ttcacgatgt
     2221 ttggcgatcg tcgcacgtac ggcttgtgca agcccagcat cctgtggctg gagatgcgtt
     2281 cgccagataa catcttcata gagcggtgcg gaacgatagg ccataatcgg gatagtaatc
     2341 taaatgataa tgattgctaa tcctagcgat aggtttatcc gatagcaagg gatttctctg
     2401 gcttgcaaat gataaaaatt atcatatgat attggttatc attatcaatg aaagagatga
     2461 aaccatgttg caacgtacgc tgggcagtgg ctggggagtg ttgctgccag gattgctgat
     2521 tgcagggctg atgtatgcgg atttatcacc ggatcagtgg cggattgtca ttctgatggg
     2581 attagtattg acgtcggtga tgctgtatca caaacagtta cggcattacg ttttgctact
     2641 atcgtgcctg gcgcttattg ctgccatcat gctgatgata atgaatttga atcagggatg
     2701 aaaaatcaag gaagaaacaa gaaaggaagt aaagataatt ggtgcgaggg gggggacttg
     2761 aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac
     2821 cttcgcatac catcaattct taaaaagaat tgctaccacg gaggcgcatt ctagtggttt
     2881 tcagcttttc gtcaatagtt aattatcgcc ggaggtgtaa ttgctggaaa aatgtccatc
     2941 aggaaactag cgtgcaggtt tggtaggcat gcgggggcag atgccagatg cgacgctggc
     3001 gcgtcttatc tggcctacga agggctaacg tgcaggtttt gtaggtcgga taaggcgttc
     3061 acgccgcatc cgacacggta ttcggcgaga taattaacct ttcttcgcct ggcgggtcat
     3121 aatggcgcga tacaccttga aacgcccggt ttgcgcaatc acttcatgga agccaaatgt
     3181 ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc
     3241 gctattaaga tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcca ggctggtttg
     3301 catcccatcg tggaacggcg ggttggagat gatcatatca aaacgacctt tcacctcgga
     3361 aaagacgttg ctggcaaaga cttcaccttc aacacagttg gccgcaagtg ttgcgcggct
     3421 ggcttctacc gctggcgcag agacatcgca caaggtgaga cgaattttcg gcgaatggcg
     3481 cgcaaaggca actgaaagca cccccgcgcc acagccgaca tccagcactt tacctttcgt
     3541 gtgcggggtt aacgtcgaga gtaacaactg gctaccgaca tccagaccgt cgcggctaaa
     3601 cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc
     3661 cgcatcaaat actggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct
     3721 gtcgacttta ttcaacggcg cataatctgc cagcatctgc tcggcgctgc gcacgccgct
     3781 gcggttctcg ccaacgacaa aaatatccgt ccccaccggc agcagagaaa gtaaattcat
     3841 caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc
     3901 tgcgacgtca tccaccgtgg cgaccagact gaaacgggcg ttatccccca tctggcggct
     3961 taatacctgc cagtggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg
     4021 cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc
     4081 actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat
     4141 tgatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg
     4201 cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa
     4261 ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt
     4321 gcgatcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgaccgat
     4381 cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt gctgcaactg
     4441 acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt
     4501 actgacgaac cgctatcact ggaaggcgct caggtggcat caccggcgct caccgaatta
     4561 cgggcaaacc caacggcacg cgccgcgtta tggcaacaga tttgcacata tgaacacgat
     4621 ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc
     4681 ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca
     4741 gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc
     4801 tacattgttc aatattgcgg tcgatcctga ctatcagcgt cagggattgg gaagggtgct
     4861 gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgactctct ggctggaagt
     4921 ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac
     4981 gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt
     5041 gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc
     5101 gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac
     5161 agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa accactgtgg
     5221 gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg tttcgagagc
     5281 tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat taatgcgatg
     5341 gcggaaatct gcacgccgct gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac
     5401 gccaaaatcg gcatcatcac caacggcttt agcgccttgc agcaagtgcg tctggaacgc
     5461 acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa
     5521 ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc
     5581 gtgctgatgg ttggcgacac tgctgagtcc gatattctcg gtggcatcaa cgccgggctt
     5641 gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcccacctgg
     5701 accgtttcat cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc
     5761 cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt
     5821 actcaagaag aaagaattat gacgttgtct ccttatttgc aagaggtggc gaagcgccgc
     5881 acttttgcca ttatttctca cccggacgcc ggtaaaacta ccatcaccga gaaggtgctg
     5941 ctgttcggac aggccattca gaccgctggt acagtaaaag gccgtggttc caaccagcac
     6001 gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg
     6061 atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac
     6121 ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac
     6181 gctgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac
     6241 acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg
     6301 ctcgatgaag ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg gccgattggc
     6361 tgcggcaagc tgtttaaagg cgtttaccac ctttataaag acgaaaccta tctctatcag
     6421 agcggtaaag gccacacgat tcaggaagtc cgtattgtta aagggctgaa taacccggat
     6481 ctcgacgctg cggttggtga agatctggca cagcagctac gtgacgaact ggaactggtg
     6541 aaaggcgcgt ctaacgagtt cgacaaagaa ttgttccttg cgggcgaaat cactccggta
     6601 ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctggatgg cctggtggag
     6661 tgggctcctg cgccgatgcc gcgtcagact gatacccgta ccgtagaggc gagcgaagac
     6721 aaatttaccg gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc
     6781 gtggcgttca tgcgcgtagt atccggtaaa tatgaaaaag gcatgaagct gcgccaggtg
     6841 cgtactgcga aagatgtggt tatctccgac gcgctgacct ttatggcggg cgaccgttcg
     6901 cacgttgaag aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg cactattcag
     6961 atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca
     7021 ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgctcaaa
     7081 gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccgat ctccaacaac
     7141 gatttgatcg ttggtgctgt tggtgtgctg cagtttgatg tggtggtatc gcgcctgaag
     7201 agcgaataca acgttgaagc agtatatgaa tcagtcaacg ttgccactgc ccgctgggta
     7261 gaatgtgcgg acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg
     7321 cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggcg
     7381 caggaacgtt atccggacgt tcagttccac cagacccgcg agcattaatt cttgcctcca
     7441 gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt
     7501 tcttaaaaca ttcacttttg cttatgtttt cgctgatatc acgagcggtt tcaaaattgt
     7561 gatctatatt taacaaagta atgacatttc tgacggcgtt aaataccgtt caatgcgtag
     7621 atatcagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc
     7681 tgtaatggtc gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga
     7741 gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gccaggctca aattacgagc
     7801 aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg
     7861 ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcacaga
     7921 ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca
     7981 tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca
     8041 agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg
     8101 aaagccaggc tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggggtgacct
     8161 ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctctgtgaag ggctacgcgg
     8221 gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt
     8281 cccgtcatgt gaaagttgaa accaccgacg gcgtggttca gctctccggt accgtcgatt
     8341 cccaggcaca gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg
     8401 ttaaaaatga tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg
     8461 aggcgcgatg tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag
     8521 cggtgacatt aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg
     8581 ttatcgcgtt aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg
     8641 cagctaaaat tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg ttcatgggcc
     8701 gaaaacgacc ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac
     8761 tggcttttgc ggttttagcg aataataaat tgcgttactt tgtcattctt tcacaacaaa
     8821 aacaggaagg cagaggtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg
     8881 tcgctaaggc cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt
     8941 ggaattttca cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat
     9001 ctttatctcg gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc
     9061 ggttacgcgc gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg
     9121 cgctttgtcc gtggaggaaa tcttatcgat ctcgactggc tggtggaggc caccgcaagc
     9181 cagatgccgt tgcaaatgga taccgccgcg cggttgtttg acagcggcaa atcgttttat
     9241 atgtgcgcct gtcgtcagga tgactacgcg cctaattact ttttaccaac caaacaaaac
     9301 tggctggatg tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg
     9361 ctggaaggca ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg
     9421 gcaaggcagg gcgctaaaac gttggtcgtc atccgcactg tgccgtcaca aatgtactac
     9481 acgccgcagt ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg
     9541 gtcaatctgg tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa
     9601 ccaccgggca agctgcggat attcgaaatt tatccaccga agccattaca tagtatcgcg
     9661 cttggcagtc ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt
     9721 tatttcctcg ccacggttgg caagctatta actgaaaaag cgccgcttac ccgccatctg
     9781 gtgccagtgg tgacgccgga atcgatcgtt attccgcctg cgccagtcgc caacgatacg
     9841 ctggttgccg aagtgagcga tgctccgcag gcgaacgacc cgacatttaa caatgaggat
     9901 ctggcttgat ttgccgtttt atcgataccc actgccattt tgatttcccg ccgtttagtg
     9961 gcgatgaaga ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag atcattgttc
    10021 cggcgacaga ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa tatcaaccgc
    10081 tttatgccgc attggggttg catccgggga tgttggaaaa acatagcgat gtgtctcttg
    10141 atcagctaca gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg ggggagatcg
    10201 gtctggatct cttcggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac
    10261 agctgaaact ggcgaaacgc tacgatctgc cggtgatcct gcattcacgg cgcacacacg
    10321 acaaactggc gatgcatctt aaacgccacg atttatcgcg cactggcgtg gttcacggtt
    10381 tttccggcag cctgcaacag gccgaacggt ttgtacagct gggctacaaa atcggcgtag
    10441 gcggtactat cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc
    10501 tggcatcgtt gttgctggaa accgacgcgc cggatatgcc gctcaatggt tttcaggggc
    10561 aggctaaccg cccggaacag gccgcccggg tatttgccgt gctgtgcgag ttgcgctcgg
    10621 aaccggcgga tgagattgcc gaagtgttgc ttaataatac gtatgcggtg tttagcgtta
    10681 gtgggtagtt tgcccttcgt aggcagcatc cggcagtcgg cgcataatgc ctgatgcgac
    10741 gcttgtcgcg tcttatcatg cctacaggtt tgtgccgaac cgtaggccgt atccggcatg
    10801 tcacaaatag agcgccggaa atatcaaccg gctcaccccg cgcaccttta acgcatcagc
    10861 caacggctca acgtcttccg gcgtggcgct cgcccagctt tgcgcctcgc catacacgcc
    10921 gtgggcatga aacgcattca ggcgaaccgg aacatcgcca agtctcttga taaacgtcgc
    10981 cagctcatcg atgtgatgca aataatccac ctggtctgga attaccagca aacgcagttc
    11041 cgccagctta ccgcgctctg ccagcaaaca gatgctgcgc ttaatctgct gattatcgcg
    11101 tccggttaat tgctgatgac attcgctccc ccacgctttg agatcgagca tcgcgccgtc
    11161 gcacaccggg agtaattttt cccagccggt ttcgctcaac ataccgttac tgtccacgag
    11221 gcaggtgagg tggcgcagtt gcggatcgtt tttgatggct gtaaacagcg ccaccacaaa
    11281 cggcagttgg gtcgtggctt cgccgccgct taccgttatc ccttcaataa acagcaccgc
    11341 tttgcggaca tggctaagca cttcgtccac gctcatggat tgcgccattg gcgtggcatg
    11401 ttgcggacac ctcttcaggc aggtatcaca ctgctcgcaa accacagcgt tccacaccac
    11461 tttgccgtca acaatctgca acgcctgatg cggacactgt ggcacgcact ccccacagtc
    11521 attgcaacgt cccatcgtcc acggattgtg acagtttttg cagcgcagat tgcagccctg
    11581 caaaaacaga gccagacgac tgcctggccc gtcaacgcag gagaagggga taatcttact
    11641 gactaaagcg catctgctgt tcatggctta tcacgcgcgg ctggcgttcc agaatacgag
    11701 tgttgcgtgc ggcttcttcg cccagccagg tggtgttggt gcgtgaacct tcggcgcgat
    11761 atttttctaa gtccgacaaa cgcaccatat aaccggtaac gcgaaccaga tcgttaccgc
    11821 tgacattggc ggtaaattca cgcattccgg ctttaaaggc accgaggcaa agctgtacca
    11881 gtgcctgcgg gttacgtttg atggtttcgt cgagcgtcag aatgtcgctg atgccggaat
    11941 aataataagc atgatgcggg gcgacagttt gcagatgggt gatcggatct ggctcatcgc
    12001 catacggcaa acgcgcgccc ggcgtggtgc cgatatcgga actgatcccc gactgtgcgt
    12061 gtaacatggc gcgtttttgc cagccatatt tcacgggggt attggcgaca aactccgcca
    12121 gttgcgcgct gatgcgataa cccacttcat ttgcggcggc ttctttaccg tagcgcgcgg
    12181 caatcccttc tttcttacac agcaagttaa ccgcttccgc cagcccatac atgccaaaca
    12241 ttggcacaaa acgttcaggg ttaatcagcc cttctttcac caggaagcta ttctcaaaga
    12301 agtgtgattg ttgatagagg aattcacacc gcgcatcgat gatggcgatc tgctgctggc
    12361 agtagtgcgg tagagtgcgc gtaaagaagt catccagcga ttcgctgcgc tcggcaatgg
    12421 ctttcaggtt aaggcgtacc agcgtgctgc caccacccgc cagcggcagt gagttgtaac
    12481 agctcacaat cccgtagccc ccttttgtga aaattttatc atgcaccgga ccgttggcga
    12541 tgtgcggttt gctacattca cagatgttct tcgccacttc cagcagcagg tcatcagggg
    12601 tgatttcagg atcgtagata aaggtcaggt tcggtgaaac ctgcttcaac tctgcgtctg
    12661 cacgtaagat cgcgcgggta atgggcgaat cagacgggcc gatattggcg tgcataaagg
    12721 cgtctggcag ggttctgtcg aggtaacgcc agaaacgttt tattcgaaca tcgatctcgt
    12781 cttgtgttag aattctaaca tacggttgca acaacgcatc cagttgcccc aggtagaccg
    12841 gcatcgatgt gaccgacggt acgtggtggt aaagaatggt cagcagagag agtgcgtcat
    12901 caagatcttt cgcgccttcc agctccagcc attcggaacc gttcgccaga aaacgggcgt
    12961 aatcgggtaa gacatagcgc ggtttgtacg gcgcatgacc ttcaaacata tcgcagatta
    13021 caccctcatc cagcgcgcgg cgggcttcgg caggaagctg cggataaggc agattgtttt
    13081 ctgcttccag agccagaaaa tggcgcttct gctccgggct aagcactggg ctggtgacaa
    13141 tttgctggca acgttgttgc agtgcatttt catgagaagt gggcatcttc ttttcctttt
    13201 atgccgaagg tgatgcgcca ttgtaagaag tttcgtgatg ttcactttga tcctgatgcg
    13261 tttgccacca ctgacgcatt catttgaaag tgaattattt gaaccagatc gcattacagt
    13321 gatgcaaact tgtaagtaga tttccttaat tgtgatgtat atcgaagtgt gttgcggagt
    13381 agatgttaga atactaacaa actcgcaagg tgaattttat tggcgacaag ccaggagaat
    13441 gaaatgactg atctgaaagc aagcagcctg cgtgcactga aattgatgga cctgaccacc
    13501 ctgaatgacg acgacaccga cgagaaagta attgctctgt gtcatcaggc caaaactccg
    13561 gtcggcaata ccgccgctat ctgtatctat cctcgcttta tcccgattgc tcgcaaaacg
    13621 ctgaaagagc agggcacccc ggaaatccga atcgctacgg taaccaactt cccacacggt
    13681 aacgatgaca tcgaaatcgc gctggcagaa acccgtgcgg caatcgccta cggtgctgat
    13741 gaagttgacg ttgtgttccc gtaccgcgcg ctgatggcgg gtaacgagca ggttggtttt
    13801 gacctggtga aagcctgtaa agaggcttgc gcggcagcga atgtactgct gaaagtgatc
    13861 atcgaaaccg gcgaactgaa agacgaagcg ctgatccgta aagcgtctga aatctccatc
    13921 aaagcgggtg cggacttcat caaaacctct accggtaaag tggctgtgaa cgcgacgccg
    13981 gaaagcgcgc gcatcatgat ggaagtgatc cgtgatatgg gcgtagaaaa aaccgttggt
    14041 ttcaaaccag cgggcggcgt gcgtactgcg gaagatgcgc agaaatatct cgccatcgca
    14101 gatgaactgt tcggtgctga ctgggcagat gcgcgtcact accgctttgg tgcttccagc
    14161 ctgctggcaa gcctgttgaa agcgctgggc cacggtgacg gtaagagcgc cagcagctac
    14221 taagtaagat gctttacgcc tgatgcgctg cgcttatcag gcctacgaga cgtatctacc
    14281 cgtaggccgg ataaggcgta gacgcatccg gcaaaagccg cctcatactc ttttcctcgg
    14341 gaggttacct tgtttctcgc acaagaaatt attcgtaaaa aacgtgatgg tcatgcgcta
    14401 agcgatgaag aaattcgttt ctttatcaac ggtattcgcg acaacactat ctccgaaggg
    14461 cagattgccg ccctcgcgat gaccattttc ttccacgata tgacaatgcc tgagcgtgtc
    14521 tcgctgacca tggcgatgcg agattcagga accgttctcg actggaaaag cctgcatctg
    14581 aatggcccga ttgttgataa acactccacc ggcggcgtcg gcgatgtgac ttcgctgatg
    14641 ttggggccga tggtcgcagc ctgcggcggc tatattccga tgatctccgg tcgcggcctc
    14701 ggtcatactg gcggtacgct cgacaaactg gaatccatcc ctggcttcga cattttcccg
    14761 gatgacaacc gtttccgcga aattattaaa gacgtcggcg tagcgattat cggccagacc
    14821 agctcactgg ctccggcgga taaacgtttc tacgcgaccc gtgatattac cgcaaccgtg
    14881 gactccatcc cgctgatcac tgcctcgatc ctggcgaaga aactggcgga aggtctggat
    14941 gcgctggtga tggacgtgaa agtgggtagc ggcgcgttta tgccgaccta cgaactctct
    15001 gaagcccttg ccgaagcgat tgttggcgtg gctaacggcg ctggcgtgcg caccaccgcg
    15061 ctgctcaccg atatgaatca ggtactggcc tccagtgcag gtaacgcggt tgaagttcgt
    15121 gaagcggtgc agttcctgac gggtgaatat cgtaacccgc gtctgtttga tgtcacgatg
    15181 gcgctgtgcg tggagatgct tatctccggc aaactggcga aagatgacgc cgaagcgcgc
    15241 gcgaaattgc aagcagtgct ggacaacggt aaagcggcag aagtctttgg tcgtatggta
    15301 gcggcacaaa aaggcccaac cgacttcgtt gagaactacg cgaagtatct gccgacagcg
    15361 atgctgacga aagcagtcta tgctgatacc gaaggttttg tcagtgaaat ggatacccgc
    15421 gcgctgggga tggcagtggt tgcaatgggc ggcggtcgtc gtcaggcatc tgacaccatt
    15481 gattacagcg tcggctttac tgatatggcg cgtctgggcg accaggtaga cggtcagcgt
    15541 ccgctggcgg ttatccacgc gaaagacgaa aacagctggc aggaagcggc gaaagcggtg
    15601 aaagcggcaa ttaaacttgc cgataaagca ccggaaagca caccaactgt ctatcgccgt
    15661 atcagcgaat aacggtatac tgatctgatc atttaaattt gaagcactga gtacggagaa
    15721 catatgaaac gtgcatttat tatggtgctg gactcattcg gcatcggcgc tacagaagat
    15781 gcagaacgct ttggtgacgt cggggctgac accctgggtc atatcgcaga agcttgtgct
    15841 aaaggcgaag ctgataacgg tcgtaaaggc ccgctcaatc tgccaaatct gacccgtctg
    15901 gggctggcga aagcacacga aggttctacc ggtttcattc cggcgggaat ggacggcaac
    15961 gctgaagtta tcggcgcgta cgcatgggcg cacgaaatgt catccggtaa agataccccg
    16021 tctggtcact gggaaatcgc cggtgtcccg gttctgtttg agtggggata cttctccgat
    16081 cacgaaaaca gcttcccgca agagctgctg gataaactgg ttgaacgcgc taacctgcct
    16141 ggttatctcg gtaactgcca ctcttccggt acggtcattc tggatcagct gggcgaagag
    16201 cacatgaaaa ccggcaagcc gattttctat acctccgctg actccgtgtt ccagattgcc
    16261 tgccacgaag aaacctttgg cctggataaa ctctacgaac tgtgcgaaat cgcccgtgaa
    16321 gagctgacca acggcggcta caacatcggt cgtgttatcg ctcgtccgtt tattggcgac
    16381 aaagccggta acttccagcg taccggtaac cgtcacgacc tggctgttga gccgccagca
    16441 ccgaccgtgc tgcagaaact ggttgatgaa aaacacggtc aggtggtttc tgtcggtaaa
    16501 attgcagaca tctacgccaa ctgcggtatc accaagaaag tgaaagcaac tggcctggac
    16561 gcgctgtttg acgccaccat caaagagatg aaagaagcgg gtgataacac catcgtcttc
    16621 accaacttcg ttgacttcga ctcttcctgg ggccaccgtc gcgacgtcgc aggttatgcg
    16681 gcgggtctgg aactgttcga ccgccgtctg ccggagctga tgtctttgct gcgcgatgac
    16741 gacatcctga tcctcactgc tgaccacggt tgtgatccga cctggaccgg tactgatcac
    16801 acgcgtgaac acattccggt actggtttac ggcccgaaag taaaaccggg ttcactgggt
    16861 caccgtgaaa ccttcgcgga tatcggccag actctggcaa aatattttgg tacttctgat
    16921 atggaatatg gcaaagccat gttctgataa tgggtgcggc ttgatgccct caccccggcc
    16981 ctctcccaca gggagagggt gaaaacatgt tgataaggat aaaacaatgg ctaccccaca
    17041 cattaatgca gaaatgggcg atttcgctga cgtagttttg atgccaggcg acccgctgcg
    17101 tgcgaagtat attgctgaaa ctttccttga agatgcccgt gaagtgaaca acgttcgcgg
    17161 tatgctgggc ttcaccggta cttacaaagg ccgcaaaatt tccgtaatgg gtcacggtat
    17221 gggtatcccg tcctgctcca tctacaccaa agaactgatc accgatttcg gcgtgaagaa
    17281 aattatccgc gtgggttcct gtggcgcagt tctgccgcac gtaaaactgc gcgacgtcgt
    17341 tatcggtatg ggtgcctgca ccgattccaa agttaaccgc atccgtttta aagatcatga
    17401 ctttgccgct atcgctgact ttgacatggt acgtaacgcg gtagacgcgg ctaaagcact
    17461 gggcgttgat gctcgcgtgg gtaacctgtt ctccgctgac ctgttctact ctccggacgg
    17521 cgaaatgttc gacgtgatgg aaaaatacgg catcctcggc gtggaaatgg aagcagctgg
    17581 tatctacggc gtcgctgcag aatttggcgc gaaagccctg accatctgca ccgtgtctga
    17641 ccacatccgc actcacgagc agaccactgc cgctgagcgt cagaccacct tcaacgacat
    17701 gatcaaaatc gcactggaat ccgttctgct gggcgataaa gagtaattgt gtttcgctga
    17761 aaagcgattg tcgtgtgaag ccggagtggg agactgctcc ggctttttag tatctattca
    17821 tca
//
LOCUS       DAPPNJ010000090        17808 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000090 DAPPNJ010000000
VERSION     DAPPNJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.090"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(50..1642)
                     /gene="lsrK"
                     /locus_tag="RPM73_002503"
     CDS             complement(50..1642)
                     /gene="lsrK"
                     /locus_tag="RPM73_002503"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDX6520850.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1721..2674)
                     /gene="lsrR"
                     /locus_tag="RPM73_002504"
     CDS             complement(1721..2674)
                     /gene="lsrR"
                     /locus_tag="RPM73_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDX6520851.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2923..4458
                     /gene="lsrA"
                     /locus_tag="RPM73_002505"
     CDS             2923..4458
                     /gene="lsrA"
                     /locus_tag="RPM73_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDX6520852.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4452..5480
                     /gene="lsrC"
                     /locus_tag="RPM73_002506"
     CDS             4452..5480
                     /gene="lsrC"
                     /locus_tag="RPM73_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDX6520853.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5480..6472
                     /gene="lsrD"
                     /locus_tag="RPM73_002507"
     CDS             5480..6472
                     /gene="lsrD"
                     /locus_tag="RPM73_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDX6520854.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6484..7506
                     /gene="lsrB"
                     /locus_tag="RPM73_002508"
     CDS             6484..7506
                     /gene="lsrB"
                     /locus_tag="RPM73_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDX6520855.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGILVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7533..8408
                     /gene="lsrF"
                     /locus_tag="RPM73_002509"
     CDS             7533..8408
                     /gene="lsrF"
                     /locus_tag="RPM73_002509"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDX6520856.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8432..8722
                     /gene="lsrG"
                     /locus_tag="RPM73_002510"
     CDS             8432..8722
                     /gene="lsrG"
                     /locus_tag="RPM73_002510"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDX6520857.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8779..9537
                     /gene="tam"
                     /locus_tag="RPM73_002511"
     CDS             8779..9537
                     /gene="tam"
                     /locus_tag="RPM73_002511"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDX6520858.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLIESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9541..10455)
                     /locus_tag="RPM73_002512"
     CDS             complement(9541..10455)
                     /locus_tag="RPM73_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDX6520859.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10662..12113)
                     /gene="uxaB"
                     /locus_tag="RPM73_002513"
     CDS             complement(10662..12113)
                     /gene="uxaB"
                     /locus_tag="RPM73_002513"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDX6520860.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVMAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12340..13758)
                     /locus_tag="RPM73_002514"
     CDS             complement(12340..13758)
                     /locus_tag="RPM73_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX6520861.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13897..14256)
                     /locus_tag="RPM73_002515"
     CDS             complement(13897..14256)
                     /locus_tag="RPM73_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDX6520862.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14256..15182)
                     /gene="glsB"
                     /locus_tag="RPM73_002516"
     CDS             complement(14256..15182)
                     /gene="glsB"
                     /locus_tag="RPM73_002516"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDX6520863.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15246..16634)
                     /gene="sad"
                     /locus_tag="RPM73_002517"
     CDS             complement(15246..16634)
                     /gene="sad"
                     /locus_tag="RPM73_002517"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDX6520864.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16735..17616
                     /locus_tag="RPM73_002518"
     CDS             16735..17616
                     /locus_tag="RPM73_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520865.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17694..>17808
                     /gene="yneK"
                     /locus_tag="RPM73_002519"
     CDS             17694..>17808
                     /gene="yneK"
                     /locus_tag="RPM73_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDX6520866.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALS"
BASE COUNT         4442 a         4573 c         4452 g         4341 t
ORIGIN      
        1 tactcaaaaa ctaacagcca cggtcatcat gatgtggctg tcaatgaaac tataacccag
       61 gcgctttcca taacgacgtc gtcagtccat gatcaaccag ccccagctga tcctgataaa
      121 ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg cttttccggg tctggtgtgt
      181 gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc cattgatgaa aaaattccgg
      241 caccgacgcc agctgcaatg gcacatccta atgcagtggc ttctttgacc accggaatat
      301 tgacgggtaa tcccgagaca tcagcgagaa tttgactcca taatttccct tttgaacctc
      361 cgcctgcaaa gactaacgat gaaggatgaa tattcgagaa atcagcaatt tgctgcaagt
      421 tacacgctga tacaatcgcc gcattttctt ccagcgcacg gaacaatgtc gctttgttac
      481 atttatccgg gtcaatggac aagttaataa aggaaggcgc agcgtgatac caggttttaa
      541 agcgcattct gtcggagaag atcggcatta cgccccacga cccaggcggc acccgactgg
      601 ccatctcttc cagcagcgta taggtgtcga tgcctaaacg ttcggcaatc agtttttctt
      661 cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc ggtaaaaaag cttatagatt
      721 cagcttgtac catgccagga ataacatgag gattaacgcg cacgttcatt tctgggtctg
      781 tcaccggcgc ggctaaattt acaacttgct gccagaatgt gccgccaaga accgcggttt
      841 gtgccggacg cacaacgcct aacccaaggc aaccaagctg cacgtcgcct cctccaacga
      901 ccaccggagt gcccgccttc agaccgcaga gttccgccgc ttgtgaactt accacgccca
      961 gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc acgcaggcca gccatatcca
     1021 gcaatgcagg tttccagtca cgggtggtta gatcaagaag tcccgtggtg ccagcgttag
     1081 agggatccac cgccagttcg ccgctgagca tataggccag ccagtcgctg atcatggtga
     1141 tggttgatgc ctgacggtaa atatcggaac gatggtgcgc cagccaaagt aatctgggga
     1201 tggcacttaa agccagtgtt tgtccggtcg cgcgataaac ttcgttttca aaggtattgt
     1261 tgtgcagttc tttaagttcg ctaacttcgc gtgccgctct ggcatccaca ttggcgcagg
     1321 cccagatcgg ggctccttca ttattatata aaacaatgcc ttcacgcatc gaacatgccg
     1381 aaacggcagc gatatactcc ggggctatgc cggcgttgtg cagcgcctgg cgcatacact
     1441 cacacgccag ttgccagttt ttgttgagat caaattccat agaaccagga acgtccggta
     1501 ctgccaggtg ccgccactcc gcctgtccca ctgctatttg attgccttcc aggtcgaata
     1561 tcacagcccg aatacttccg gtacctgcat ccagcgccat caggtaatac tttgattctg
     1621 aaggggtaaa gagtcgagcc attagattat cctcggctta tgcgttctgt ctgttcctct
     1681 atacgttctc catcattccc ggtaataagg tcatgcaaat ttaactacgt aaaatcgccg
     1741 ctgctgtgtc ctgatcggta accagtgcgt tgatataacc gcctttcatt gcagcggcaa
     1801 ttgcttcggc tttattttct ccccctgcca cgccaacccg gacgggtatg gtcttcagcg
     1861 agcttaaagg taagccaatc agttcgttat gtattttaat atccgtgaca acgtcacctt
     1921 ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc ttttcgacca atcattaact
     1981 gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc gtcgtcctgt tgactgacgg
     2041 ccccaatgcc gacaatcgcc acatcggctg cttgcgcggc taacagaaca tctttgacgc
     2101 aattttcatt ttttagcgta cgggcaatgt cagcggagga tgcccgcaac ggagccggaa
     2161 taatattcac actgcacgcc gcgttaagct gcccgatacc cgtcatataa gaaccgacgc
     2221 caccggagag cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt aagcgttgca
     2281 gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt tgaagtaaac
     2341 tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc gacatcagca tccgcaagcc
     2401 cagggatcac ccggacatgt tgcagcgaaa actgacgacg taattgagtt tcatattcca
     2461 gacagccttc aaagcgagaa ttaatctgta cgcgaataat gccggactga tgccctttct
     2521 ccagcaagcg cgacactttc aaacgggtca ggccgagacg atcgctgatc tcgctctggg
     2581 tcagcccgtc gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct tcacacattc
     2641 cctgttctga aattgccgaa tcgttgattg tcataattca ttcttcactt tgaacatatt
     2701 taaatcttta atgcaattgt tcagttcttg cgcatttata tctgtgatgg cagccacagt
     2761 ttgactctac gagcatgaac aaacgcaacc gtgaaaatca aaatagcata aattgtgatc
     2821 tattcgtcgg aaatatgtgc aatgtccacc taaggttatg aacaaattaa aagcagaaat
     2881 acatttgttc aaaactcacc tgcaaaactg aacgggggaa atatgcaaac gagtgatacc
     2941 cgcgcgttac cgctactttg cgcccgctcg gtttataaac agtattcagg ggtcaatgtc
     3001 ctgaaaggca tcgattttac gttgcatcag ggggaggtcc acgccctgct cggcggcaat
     3061 ggtgccggta aatcgacgtt aatgaagatt attgccggta ttacccctgc tgatagcggt
     3121 acgctggaga ttgggggcaa caactacgcc agattaacgc cagttcatgc tcatcagctg
     3181 ggcatttatc tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat aaaagaaaac
     3241 atcctgtttg ggatggcaaa aaaacagctc tccatgcaaa aaatgaagaa cttgctggcg
     3301 gcgctgggct gccagtttga tctgcatagt ctggcaggat cgctggatgt cgccgatcgc
     3361 caaatggtgg aaatcctccg cgggctaatg cgcgactcgc ggattctgat cctcgatgaa
     3421 cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt ttactcgctt gcaagagctg
     3481 cttgctactg gcgtgggtat tgtttttatc tcgcataagc tgccggaaat tcgccagatt
     3541 gccgatcgaa ttagcgtgat gcgcgacgga accatcgcct taagcggcaa aaccagcgaa
     3601 ctgtctaccg acgacattat tcaggccatc acgccagtgg tacgggaaaa atcgctctct
     3661 gccagccaaa aattatggct ggagttacct ggtaaccgcc cacaacatgc tgccggaacg
     3721 ccggtgctga cactggaaaa tctgaccggc gaaggtttca ggaatgtcag catgacgctc
     3781 aatgccggag aaattctggg cctggctggg ctggtggggg ccggacgcac agaactggcc
     3841 gagacgctct atggtctgcg tactttgcgt ggcggacgca ttatgctgaa tggtaaagag
     3901 atcaataaat tatccactgg agaacgttta ctgcgcggtc tggtttatct gccggaagat
     3961 cgccagtcat ccggactgaa tctcgatgct tcgcttgcct ggaacgtctg cgcccttact
     4021 cataaccttc gtggattctg ggcgaaaacc gcgaaagata atgccaccct ggaacgttat
     4081 cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag ctgcacggac attatccggt
     4141 ggcaaccagc aaaaaatcct cattgccaaa tgcctggaag cttcgccgca agcattgatt
     4201 gtcgatgagc cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta ccagctgttg
     4261 cgcagcatcg ccgcacaaaa tgtggcagtg ctgcttatct cctccgatct ggaagagatc
     4321 gaactgatgg cagatcgcgt gtatgtgatg catcagggcg aaattgccca ctctgcactg
     4381 accgggcgcg atattaatgt cgagactatt atgcgcgttg ccttcggcga tagtcagcgt
     4441 caggaggtgt catgctgaag tttattcaga acaaccgtga aatcacggca ctgctggcgg
     4501 tggtgctgct gtttgcatta cccggttttc tcgaccgcca gtatttaagt gtgcaaacgc
     4561 tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc gcgacgctgg
     4621 taatgcttac gcgcaatatt gatgtttcag tgggttcgat taccggaatg tgcgcagtgc
     4681 tgttggggat gttactgaac gcggggtatt cactacctgt tgcttgtgtc gcgactttac
     4741 tgcttggttt gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta aagatccctg
     4801 ccattgttgc cacccttggc acgttagggt tgtacagagg catcatgttg ctgtggactg
     4861 gcggtaaatg gattgaaggg ttacccgccg aactgaaaca gctctccgca ccgctgctgt
     4921 ttggcgtttc cgcgattggt tggttgacga taattctggt ggcatttatg gcctggctgc
     4981 tggcaaagac ggcgtttgga cgcagttttt atgccacggg cgataattta cagggcgctc
     5041 gtcaactggg tgttcgtacg gaagccattc gcattgtggc attttcgttg aacggctgca
     5101 tggcggcact ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc aaccagaccg
     5161 gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt agtttgctcg
     5221 gcggttccgg tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg cagatcgata
     5281 gcgtactggt gctgttgcgc attccggcat ggtggaacga ttttatcgcg ggcctcgtgc
     5341 tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt aatctacggc
     5401 ggcaaaaata tgcccgcttt atgacgccac cgccatccgt taaacccgct tcgtcaggta
     5461 aaaaacggga ggccgcataa tgcgtattcg ctacggttgg gaactggctc ttgccgcact
     5521 gctcgttatt gagattgtcg catttggtgc aattaacccg cgaatgttag atctcaatat
     5581 gttgctgttc agcaccagtg actttatctg cattggcatt gtcgccctac cgctgacgat
     5641 ggtgattgtc agtggcggga tcgatatttc gtttggttcg accatcggcc tctgcgccat
     5701 tgcattgggc gtactgtttc aaagtggtgt gccgatgccg ctggcgatac tcctgacctt
     5761 actgctcggc gcattgtgcg ggctgatcaa cgccggatta attatctata ccaaagttaa
     5821 cccgctggtg attacgcttg gcacgctgta tctgtttgcc ggaagcgctc tgctgctttc
     5881 cggtatggcc ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg cgtttacaga
     5941 tttcgctaac ctggatgtgc tgggactccc cgttccgctg attatcttcc tgatatgtct
     6001 cctcgttttc tggctctggc tgcataaaac ccatgccgga cgtaatgtgt ttttgattgg
     6061 gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt aaccgcacct tatgtgcgct
     6121 ctatgccatg acggggctgg cgtctgcggt cgccgctgtg ctgctggtat cgtattttgg
     6181 ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg ccgtggtgct
     6241 tggcggtgcc aatatttatg gtggttccgg ttccattatc ggcaccgcca ttgcggtttt
     6301 attagtggga tatttgcaac aaggtttgca aatggcagga gtgccaaatc aggtgtccag
     6361 cgccctttcc ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta gcctgcatcg
     6421 ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac ccattgccat aaaggatatc
     6481 ttcatgacac ttcatcgctt taagaaaatc gccttactta gcgctcttgg cattgccgca
     6541 atctctatga atgtgcaggc cgcagagcgt attgcattta ttcccaaact ggttggcgtg
     6601 ggatttttta ccagcggtgg caacggcgca caacaagcgg gtaaagagct gagcgttgat
     6661 gtgacctacg acgggccgac agaacccagt gtttctggtc aggtacagtt gattaataac
     6721 ttcgtcaatc aaggttataa cgccattatc gtttctgcgg tttcgcctga tggcttgtgt
     6781 ccggcactga aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgatact
     6841 aaaccggagt gccgctctta ctacattaat cagggaacgc ccgcccagtt gggaggtata
     6901 ttggtggata tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac
     6961 tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc
     7021 aaagagcatc ctggctggga aattgtcact acgcagtttg gctataacga tgccactaaa
     7081 tcattacaaa ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc
     7141 cccgacgcca acgccctgcc cgctgccgca caagccgcag aaaacctgaa aaatgacaaa
     7201 gttgcgattg tcggattcag tacgccaaac gtgatgcgtc catatgtgga acgcggcacg
     7261 gtgaaagaat ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt gtatgtcgcg
     7321 gatgcattat tgaaaaaagg atcaatgaaa acgggcgaca agctggatat ccagggcgta
     7381 ggtcaggtgg aagtctcgcc aaacagcgtt cagggctatg actacgaagc ggatggtaat
     7441 ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat
     7501 ttctgatgtg cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg
     7561 gtaaagattt tcgtaccgat caaccgcaac aaaatatccc ttttaccctg aaaggttgcg
     7621 gtgcgctgga ttggggaatg cagtcacgct tatcgcggat atttaatccg aaaacgggta
     7681 ataccgtgat gctggccttt gatcatggtt attttcaggg gccgactacc ggacttgaac
     7741 gcattgatat aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg
     7801 gcattttgcg cagcgtagtt cccccagcga ccaataagcc ggtggtactg cgggcgtcag
     7861 gtgcgaactc tattctggcg gaattaagta atgaagccgt ggcgttatcg atggatgatg
     7921 ccgtgcgcct gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac
     7981 atcagtcgat caaaaatatt attcagctgg ttgatgccgg aatgaaagtg ggaatgccga
     8041 ccatggccgt gactggcgtg ggcaaagata tggtgcgcga tcagcgttat ttctcgctcg
     8101 cgactcgaat cgccgctgaa atgggggcgc aaattatcaa aacctattat gtcgaaaaag
     8161 gttttgaacg gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat
     8221 taccggagcg cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg
     8281 tggatatggg gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac
     8341 aggcggtggt tcaccataac gaaacggctg atcgggcata tgaactctat ctgagtgaaa
     8401 aacagtaact gcggatttaa ggagaagaat tatgcacgtc acactggttg aaattaacgt
     8461 tcatgaagac aagattgacg agtttatcga agtttttcgc cagaaccacc tgggctctgt
     8521 acaggaagaa ggcaatttgc gcttcgatgt cttacaggac ccagaagtga attcgcgctt
     8581 ttatatttac gaagcctata aagatgaaga cgcagtggca ttccataaaa ccacgcctca
     8641 ctacaaaacc tgtgtcgcga aactggaatc tttaatgacc gggccgcgta aaaaacgtct
     8701 gttcaatggg ttgatgccgt gaggcgaatt tatcattttt atctacaatt ggggtaacgc
     8761 gctgacggga gtaaaaatat gtctgactgg aacccctctt tatatctgca ctttgccgct
     8821 gaacgatcgc gtccggcggt ggagctgctt gccagagtgc cgctggagaa tatcaaatac
     8881 gttgccgatc ttggctgtgg cccaggtaac agcaccgccc tgctgcatca acgttggcct
     8941 gcggccagga taacaggcat cgactcgtct ccggcgatga ttgctgaagc gcgcagtgct
     9001 ttgccagact gccagttcgt ggaagcagac atccgcaact ggcaaccgga acaggcactc
     9061 gatctgattt ttgctaatgc ctcactgcaa tggctgcccg accactacga attgtttcct
     9121 catctggttt ctttacttaa tccgcagggc gtgctggcag tacagatgcc agataactgg
     9181 ctggagccga cccatgtgct catgcgcgaa gttgcctggg aacaaaacta cccggatcgc
     9241 gggcgtgagc cgttggctgg cgttcatgct tactacgata ttttgagcga agccggatgt
     9301 gaggtcgata tctggcgaac cacctactat caccagatgc cgtcacacca ggcgattatc
     9361 gattgggtga ctgccactgg attacgtccg tggttacagg atctgatcga gagcgaacag
     9421 cagctttttc ttaagcgcta ccatcagatg ctggaagagc agtatccact gcaagagaac
     9481 ggacagatac tgctggcatt tccgcgtctt tttattgttg cccggcgtat ggagtaaata
     9541 tcacgtcagc tggtaatgac gatcgggaag aatttttgct ggaatttcgg cttcatcgtt
     9601 catctgtaac aggtcaattt caatagcgtt gcagatggca tccagcggta aatcattgtt
     9661 ttcagtaccg aacggatctt ccagttcttc cgccagacaa tccagcgaaa taaaagtgta
     9721 ggaaatcagc acagagataa aaggcgtcat gtaatgcagg tccacgacca gcgcgaacgg
     9781 cagcatgata caaaacagat aaacggtacg atgcaaaatc agggtgtagg caaagggaat
     9841 tggcgtatag gcaatgcgct cgcatcctgc caggaccgct gaaatatcat taagtcgatc
     9901 gttgaggcta ataaacagga tatctgaaag ctgcccattg cggcgctgaa ccgccaacca
     9961 ctcccccatt attaacaaga tacggttagc tggagagttc gaagccagta caggctgaag
    10021 atcttcagtc ttgagataat gagccagcac ttccgcctgt ggctgtttgc gtaatgtcat
    10081 gcgtaaacag tgggcgaagg cgatttgcaa ccgggcaaac tcccttacac ttgccgaatc
    10141 cggcaatgtc gtttttacct cgcgcagtaa cgaccgtgag gcaatcatca actgccccca
    10201 aagttttcgc gcttcaacgt aacgggcgta cccggcatta ttacgaaaac caagaaaaat
    10261 ggcgatggcg acaccgagaa tgctgaacgg tgcgagggtg aatttgatgc ccagatgcgt
    10321 gtaccagggc agcatgaaaa taacagcgat agaaaagaga aaattgagta gtaagcgcga
    10381 tgatatcttg gataatactg agccgtgcca gacaaaaata cggcgcagcc agtgttgttg
    10441 tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc gccctattaa acgtgattac
    10501 gatcacattc tcaagacgct acttacaaat tacctacaag cttacaacat taacaggcgc
    10561 tgcatgtggc atctgttaat gataaaggca cataggatgt tgtaactaat atagtgaaat
    10621 aagaaacccg gtcgaaaccg ggttcagaag taacggtgtt attagcacaa cggacgtaca
    10681 gcttcgcgca tccctttttc aagaatcgca tccaggtcat tagcaacctg ctcgaccaga
    10741 ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt ctgccatcac aatcgctacc
    10801 agttcctggg taccgataac acggtcacga tgctggctcc agagttgctg ataacgttcc
    10861 agccagtgtg catcgtcctg taccggatat gtttctccgt tacgctcacc acgatagaag
    10921 gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccttt tgccttctgc
    10981 cctgccagca gctgcggcag gatgcgagtg cggaacttgg tcataccgtt gagcgcgata
    11041 gacagcagct gatgcttaat gtacgggtta cggaaacgcc cggtgactgc actggcgaaa
    11101 gattccagtt catcacgagg caaatccagt accggaataa tttcttcgta gatagctttt
    11161 tcaacgaatg cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc
    11221 tggaacgcca ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct
    11281 ttgtacggtt taatatcgtc aacaatcagc acgttgagcg gatatttgtc cagacgcagt
    11341 tcagtcgcta aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg
    11401 aggaaaccat cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa
    11461 ccggtaacga tacggtccac cagcgtagaa cagaagctgt tagcctgatc caaccattga
    11521 ataaatgctt ctggcagtgc ccactcttga gcatagcgca gcaccagttc acgcaacgcg
    11581 tcgccattgt agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg
    11641 ttgaaatggc taaaacgttc gaacagcaga cgggtcagtt ttgcgggata gcttaccgct
    11701 ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac ccgcttcggt ggtgttagag
    11761 aaaacaaagc gcatttccgg attgtgcgcc agtttcagga attcatcgta ttcactgtag
    11821 acgctgattt cacgattaac agagcgaatc agacgcgcgt cgctgaccgc ttcccccttc
    11881 tcattcaggc cacggataat ggtggtgtac agaccatcct gcgtgctcag tgacggcggg
    11941 aatgaagttt caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc
    12001 aggagatcga tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg
    12061 atacgttctg gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt
    12121 tcccttctga ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat
    12181 acgccgaacc cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt
    12241 gtggcgcgga tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag
    12301 aacacattgc attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta
    12361 acgcatagtg ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc
    12421 ttcgaccatc aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc
    12481 gacgccaata cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc
    12541 aacgccttta cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac
    12601 tgcaacagca aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt
    12661 ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc
    12721 gttaatgctt ttgaaatagt caatatcaag cagcatgacc gtcagatgtt gtgtctgttt
    12781 cagcgatgga cttttcagcg cctcataaag accggaccgt gagtaaacct gagtcagaaa
    12841 atcaaagtcg gctcgcagcg caacttgctt cattagcgaa ttgatcgctg ccacgctaaa
    12901 agaaaccata attgggcata tcgccatcgt ggcaataccg agacgtgcgg agaacatttc
    12961 cggaatggag aacggcgaac cgaccgagat atcaatcacc gaatttgcca ccagtacgat
    13021 ttccaccgca ccggtgacaa aggtcaacag acatgttacc tgcggcgtat agcgcactgc
    13081 acaccagatt aatgcaggca gcgggaacgc cagactcccc gctccgccaa tgactaccga
    13141 ggcaataacc gacacaataa gcgcgatggc gggcatcatc tgttctgctt taaagcgcgg
    13201 cagcactcca ggaatagcca acgtcagcat acaaggcacg atcaacacgc ccgttgagaa
    13261 ttgctcactg aaccagtccg caagcaaagg ccagaaatcc agactatcaa tactgaccga
    13321 accaatcgcc ccgacaatag cgcataataa tgcggcaatc agacagtaat taaatagccg
    13381 taaggcactt actggctcat acttattttt ccccagacgc ttatcccgca cgaccagtaa
    13441 ggcgacggta acaataaaca tcatattgga gaaattgata accagtgaaa ccagtcccca
    13501 ttcggtggtt atggcatcat aaaccagcat cgccacataa ctgatcgcat agtaatgcag
    13561 tcgattaagc cagacatagc gggcaaacac ccccgccatt acgccattca gcggccagaa
    13621 aagagaaagt tcgtgaacca gccgtaactc tgcaccaata aaatagaaca gcgtggttag
    13681 cgcaaaaata gcgatgctat tacgtagcag atgaccttcc tggaacaaac ggaaagttga
    13741 aatggggtgt acatgcatta attatctgtc catacaattg cgggtggcaa ttatctcaac
    13801 gtaataagta aattcacata aaggatagca tgttcgctaa cagcggcggc aaataaaggt
    13861 accctgggaa tgctatttta tcgttttccg caattctcat ggctgattca tctgtacaac
    13921 cagccagtga tagattaccg ccacgatata acgttgttgt tcttcgctta acgaaacacc
    13981 ctgcggaatg ttatgccact tagccagaca accgcgacag caggtcgcgg tggcgtgctg
    14041 agcgataaac accggatgcc cgcgcatggg ggtttgctta ccgtcattag ccggtaatgc
    14101 cggagcaaga cgtttagcga caaaatccgc cgcatgttgt tcgatgactg gcgcgccttt
    14161 ctccaggcaa tactgacgct ctttcatgcc cagacgaaag cgagagcgaa attttgaacg
    14221 ggataaacgc gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg ccctaactgt
    14281 ttcgtcaatt gttcaaggac ggcgataccc gcaagcgagt tacctgcatc atccagttcc
    14341 ggactccaga ctgcgatggc catttcatgc ggaacaatcg ccacaatacc gccaccaacg
    14401 ccagatttcg ccggtagtcc cacccgccag gcaaactccc ccgcgttctg gtacatacca
    14461 ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcaccac aggttcatca
    14521 atatgaatag ctttcccctg attagccaga aagacaaacg tccgggccag ctctacacag
    14581 ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac gtcatgatgg
    14641 aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga atgttcaaat
    14701 tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca caccgcttaa gccgcgcacg
    14761 acttccagca tacgttgccg tggtgcgctt agtcgccctt gcaacatatc gcagaccacc
    14821 agcgcaccgg cattaatgaa cggattacgc ggaataccct gttccatttc cagttgcact
    14881 aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat ttcctcttcg
    14941 gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg aatggaaaaa
    15001 cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat agcaatcccc
    15061 aatcgggaac cgtctactgt agccagcgcc ggaatataat ccgcgacttt accctgacca
    15121 atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat ggcgactgcc
    15181 actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga gctgaatatg
    15241 tcgcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg taagccgaaa
    15301 tgggaaagct cacgaccaaa gccactcttt ttcacgccac caaaggccac tcgcgcgtcg
    15361 ctggcacaat aaccattgat aaacacccca ccgcattcca gacgtgccgc catctgtctg
    15421 gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact atcattagcc
    15481 agttccagcg catgttctgc atctttcgca acggtgattg ccgcaacagg gccaaacatt
    15541 tcttcccgaa acgcggtcat ttctggggta acattcgcca gaaccgttgg cggatagtag
    15601 ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg cgccagggtt
    15661 ttctccacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg tccgagagcg
    15721 ttctcttcat cacggggatc gcccattttc aaggctgccg cagctgccac aaaacgttcg
    15781 gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc acaaacctgt
    15841 ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag atcggcatcg
    15901 ttaagcacaa taaatggatc cgaaccgccc agttccagta cgcatttttt cagtgccgct
    15961 ccagcctgtg cgccaatagc cgctcccgca cgaacacttc cggtcaccgt gacagcagca
    16021 atgcgcgagt ctttaatcat ctgactgaca ccgtcgttgt cggcattcag ccagccatat
    16081 acgccttgtg ggattcccgc atctttaaac acctgggcaa tgagctgagc acagcccatc
    16141 acattcggcg catgtttaag taagtaaccg ttacctgcaa gaatgatggg aaccgcgcca
    16201 cgcatcacct gccataacgg aaagttccac ggcataatcg ccagaatcgt ccccaacggt
    16261 cgatactcaa taactgcctg ctgattttcc accagcgtag gttccgcctt cagcattgcc
    16321 ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc
    16381 tgattgattg gtttgcccat ttcacgggtg atcatttgcg ccatttcttc gctacgggcg
    16441 cgcagagcct taccgatatc acgcagtttt tcagcacgat aatctatatt tgtctcgcgc
    16501 cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt cgatatcgtt agcgccagcc
    16561 cacggcagca cagaaagttg ttcacccgtg gcaggattta tcgaaattgc atgagttgcc
    16621 ggagtaatgg tcatcggggt atctccttta tgagtcatgg tatgaagata cgcagattta
    16681 ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa tattatggat
    16741 ctgacccaac tggagatgtt caacgcggtt gccgaagctg gcagcataac ccaggctgca
    16801 gcaaaagtgc atcgcgtgcc gtcgaatctc actacccgtc tacgccagct ggaaacagaa
    16861 ctgggggtag atctgtttat tcgcgagaat cagcgtttac gtctctctcc tgccgggcat
    16921 aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc
    16981 gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac cgcagcagtg
    17041 cgcattccgg ccacgctggc ggaattcaac catcgttatc ccaaaattca gttttcactt
    17101 tccaccggcc cttccggcac tatgctggaa ggagtgctgg agggaaaact gaatgcggca
    17161 tttattgatg ggcccattaa ccatactgcc attgacggga taccggtata ccgcgaggag
    17221 ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc gcgccagtca ggttaacggc
    17281 agtaacattt atgccttccg cgccaattgt tcgtatcgtc gccacttcga gagctggttt
    17341 catgctgacg gtgccgctcc gggaactatc catgaaatgg agtcttatca cggaatgttg
    17401 gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctctatgct ggaaagtatg
    17461 ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg gttaacaacc
    17521 tggctggtct ggcgtcgcgg tgcgaaaacg cgtccgcttg aggcatttat tcaactactg
    17581 gatgtgcctg actcggcaaa acagggttac caatgagcta tttttgatag ttcttgcagc
    17641 tcaaatgccc tatatagtga tgttccgatg acttatgact atatggggca aatatggtta
    17701 cgccagtaag catcagtaat tacatatctc ttcctgatga ttttcccgta cgtaatattg
    17761 cacctcaggt aaaagaagtt ttaaaggact ttattgatgc gctaagta
//
LOCUS       DAPPNJ010000091        17511 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000091 DAPPNJ010000000
VERSION     DAPPNJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.091"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(40..1323)
                     /locus_tag="RPM73_002520"
     CDS             complement(40..1323)
                     /locus_tag="RPM73_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDX6520867.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1436..3370)
                     /gene="yeaG"
                     /locus_tag="RPM73_002521"
     CDS             complement(1436..3370)
                     /gene="yeaG"
                     /locus_tag="RPM73_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDX6520868.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3806..4552
                     /gene="mipA"
                     /locus_tag="RPM73_002522"
     CDS             3806..4552
                     /gene="mipA"
                     /locus_tag="RPM73_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDX6520869.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4642..5496
                     /gene="yeaE"
                     /locus_tag="RPM73_002523"
     CDS             4642..5496
                     /gene="yeaE"
                     /locus_tag="RPM73_002523"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDX6520870.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5547..6431)
                     /locus_tag="RPM73_002524"
     CDS             complement(5547..6431)
                     /locus_tag="RPM73_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDX6520871.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6515..7510)
                     /gene="gapA"
                     /locus_tag="RPM73_002525"
     CDS             complement(6515..7510)
                     /gene="gapA"
                     /locus_tag="RPM73_002525"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDX6520872.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7852..8265
                     /gene="msrB"
                     /locus_tag="RPM73_002526"
     CDS             7852..8265
                     /gene="msrB"
                     /locus_tag="RPM73_002526"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDX6520873.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8307..8579
                     /locus_tag="RPM73_002527"
     CDS             8307..8579
                     /locus_tag="RPM73_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDX6520874.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8740..9381)
                     /gene="pncA"
                     /locus_tag="RPM73_002528"
     CDS             complement(8740..9381)
                     /gene="pncA"
                     /locus_tag="RPM73_002528"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDX6520875.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(9392..10408)
                     /gene="ansA"
                     /locus_tag="RPM73_002529"
     CDS             complement(9392..10408)
                     /gene="ansA"
                     /locus_tag="RPM73_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDX6520876.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10575..12431)
                     /gene="sppA"
                     /locus_tag="RPM73_002530"
     CDS             complement(10575..12431)
                     /gene="sppA"
                     /locus_tag="RPM73_002530"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDX6520877.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12592..13143
                     /locus_tag="RPM73_002531"
     CDS             12592..13143
                     /locus_tag="RPM73_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDX6520878.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13260..14303
                     /gene="selD"
                     /locus_tag="RPM73_002532"
     CDS             13260..14303
                     /gene="selD"
                     /locus_tag="RPM73_002532"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDX6520879.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14308..16269
                     /gene="topB"
                     /locus_tag="RPM73_002533"
     CDS             14308..16269
                     /gene="topB"
                     /locus_tag="RPM73_002533"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDX6520880.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            16396..17436
                     /locus_tag="RPM73_002534"
     CDS             16396..17436
                     /locus_tag="RPM73_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDX6520881.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHDIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         4385 a         4273 c         4513 g         4340 t
ORIGIN      
        1 ctaaaaatca gccaggcgat aatgacctgg ctgatagttt tagtctttag ctgttgcatt
       61 ttgtttatga aacagttcac ggaacaccgg ataaatatca tcctggtcgc ggatgtgctg
      121 catcgcaaag ttgtcgaaag tagattgcag atgctcatat tctcgccaca atgtctgatg
      181 tgcacgacgg gtaatttcga tatagctgta ataacgaaca acaggtaata attttttcgc
      241 caggatttca tggcaaagcg gagagtcatc ggcccagtta tcgccgtccg atgcttgtgc
      301 agcgtaaata ttccactgtg ccgggttata acgctctttc actacctcat ccatcagttt
      361 cagggcgctg gaaacgatgg tgccgcctgt ttcctgcgag tagaaaaact catgttcatc
      421 gacttctttc gcctgggtat gatggcggat gtatacgact tccacgttct tatacgttct
      481 gctgaggaac agatacagca gaatataaaa acgcttagcc atatctttag tggattgatc
      541 cattgaaccg gaaacgtcca tcaggcaaaa catcactgcc tggctggagg gatcgggccg
      601 cttctcgtag ttcttgtaac gtaaatcgaa ggtgtcaata aaagggacgc gttcaatttt
      661 ggcacgtaat tctgcaattt ctttgcgcag acgttcctct tccagcagct gcgcaggttc
      721 actgttgctg atgatggcca aattctcttc cagtgcatga agttcccgcc gcttgcctgc
      781 cgtcatggct gtgcgtcgcg ccagtgagtt ctgcaaggag cgcacaacgc tgatatttgc
      841 cggaacgccg ttagcggtat aacccgcccg atgcgtttta tattcggtca gctggcgttg
      901 ttggttttgt ttcagattcg gtaaggccaa atcttcaaag agcaaatcaa gatactcatc
      961 tttcgaaatc tgaaagacaa attcatcctg accttcacca tcctggctgg cctggccctg
     1021 accactgccg gaacctccgc cgccaccctg aggacgttca attcggtcgt tctggacgaa
     1081 atggtcattg cccggatgca cgcggtggcg cagaccgcca cgcccctgat gaaacatcgg
     1141 ttcgctaata tcttccgtgg gaatggatac ggattcgccg ctgtcgacgt cagtcaccga
     1201 acgcttatta atggcctcgg agatcgactg tttaatttgc gctttataac ggcgtaaaaa
     1261 acgctggcga ttcaccatgc ttttgttttt gccgttcaga cgccggtcaa taaaccaggt
     1321 catatgcccc ccgtactaca tttgccaact gtatgctacg cctgtgttgc cgcttctcag
     1381 gcccacggtt cgctgtcgtt gtaggcccgg taacatgtgt accgggccag ggttgttaag
     1441 acgatttacg tacgcgcaaa taccattcgc acagtaaacg cacctgttta cgggtgtagc
     1501 ctttttccat catacggtcg acaaagtcgt cgtgtttctt ctgctcgtcg gttgacgttt
     1561 tggcgttaaa cgagataacc ggcaacagct cctcggtatt ggagaacatt ttcttctcga
     1621 taaccgtgcg cagtttttca tagctggtcc agttcggatt gcgtccgctg ttattcgctc
     1681 tggcgcgcag tacgaagtta acaatctcgt tgcggaaatc ttttggatta ctgatccccg
     1741 ccggtttctc gattttctcc agctcggcgt taagagactc gcggtcaaac agctgcccgg
     1801 tatccggatc gcgatactcc tgatcctgaa tccagaaatc cgcgtaggta acataacggt
     1861 cgaaaatgtt ttgcccatat tcggaatagg attcaaggta ggccgtctgg atctctttgc
     1921 cgataaactc ggcatatttc gggatcagat aacctttcag gaactccaga tagcgttctg
     1981 cctgctcttg tgggaactgc tcgcgctcga tctgctgttc cagtacgtag aacagatgga
     2041 ccgggtttgc cgccacttct acatgatcga agttgaacac gcgggagagg atcttaaacg
     2101 caaaacgcgt cgacagaccg ttcatccctt catcgacacc ggcgtagtca cgatattcct
     2161 gatacgactt ggctttggga tcggtgtctt tcagactttc gccatcataa acccgcatct
     2221 ttgaataaat gctggagttt tctggctctt taaggcgcga aagaatggaa aaacgtgaca
     2281 gcgtttccag cgtgccaggg gcgcaggggg cgtgagtcaa ttcactgtga ttaagcaatt
     2341 tctcgtagat tttgatctct tcggaaatgc gtaagcaata cggcaccttc acgatgtaaa
     2401 cacggtcgag gaaggcttcg ttgtttttgt tattacggaa agtgacccat tcggattcgt
     2461 tcgagtgggc gagaataatc ccgttgaacg gcagggcgga gataccttcc gtcccgttgt
     2521 agttaccttc ctgagtggcg gttaacagcg gatgcagcac ttttatcggt gctttaaaca
     2581 tctcgacaaa ttccatgatc ccttggttgg cgcggcacag cgcaccggaa tagccgtagg
     2641 cgtccgggtc attctgcgcg tagtgttcga gtttacgaat atcgactttc ccaaccagcg
     2701 cggagatgtc ctggttgttc tcatcaccgg gttccgtttt ggcgatagca atttgttgca
     2761 gaattgacgg ccagaccttc actacccgga acttagtgat atcgccacca aattcatgca
     2821 ggcgttttgc cgcccatggc gacatgatgg tgccgagata acggcgagga atgccatact
     2881 ctttttccag aatctgagca tcttcctgcg gattgaaaag acagaacgga tgatcgttga
     2941 ccgggctacg ctcaccgttg gcgctcaata cataaatcgg tacgagctgc attaatgatt
     3001 tcagtcgctc agcaagcgat gatttacccc cacccacagg ccccagcaga tacaggattt
     3061 gtttcttctc ttccagcccc tgagccgcgt gtttcagata agagacaatc tgttcaatcg
     3121 cgtcttccat gccgtaaaac tcttcaaatg ccggataacg tgcaatgacc cggttagaaa
     3181 agagtcgaga aagtctgggt tcctgggctg tatcgaccat gacaggctca ccgatagcca
     3241 tcaatagccg ctcagccgcg ttggcataag cactgcgatc ttgccgacaa gtggtaagaa
     3301 actcctgcag tgtgaactct tcgtccttgg cagcttcata tcgctggcga tagtgatcga
     3361 atatattcat ggtatgccgt cctttcgttt tttagcacag gttaagagcc gttcatatga
     3421 atagtagagg ctcccggaag aggtaaactg aacgacgtct cttgctaaaa cacagcaact
     3481 ctcatgccat tttatcgacg atgagaacaa cgtgcccggt gatacacctt caaaattaag
     3541 cgtagatggc aaatggaaaa cttgcctgca cggctttctt aatttcaatg gcatatcaac
     3601 aactcatcca taaattttgc ataattaatg taaagaccag ggtagccagt aacgctaaat
     3661 tcatttggct gtaagcgcgg tgtcatccgc gtcaggaaaa ttaaacagtt actttaaaaa
     3721 atgaaaacgt aaaaaggttg ggtttcgatg tattgacggg taaactttgt cgcccgctaa
     3781 acatttgttt aaggaatgat taattgtgac caaactcaaa cttctggcac ttggagtgct
     3841 tatcgcaacg tctgcaggcg tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt
     3901 aggtgtcgtt gagcacccat ataaagatta cgataccgat gtgtacccag taccggtaat
     3961 caactatgaa ggcgataact tctggttccg tggcttaggt ggtggttact acctgtggaa
     4021 tgacgcaacg gacaaacttt caattaccgc ttattggtcg ccgctttact tcaaagcgaa
     4081 agacagtggc gatcaccaaa tgcgtcacct ggatgaccgt aagagcacca tgatggctgg
     4141 tctgtcttat gctcacttta cccagtacgg ttacctgcgt accaccctgg ctggcgatac
     4201 cctggataac agcaacggca tcgtctggga tatggcctgg ttgtatcgtt acaccaacgg
     4261 cggcctgacc gtgaccccgg gtatcggtgt gcagtggaac agcgaaaacc agaacgaata
     4321 ctattatggc gtatcgcgca aagagtccgc tcgcagcggt ctgcgtggct ataacccgaa
     4381 cgacagctgg agcccttacc tggagctgag cgccagctac aacttcctcg gcgactggag
     4441 tgtttacggt accgcgcgct atacccgtct gtctgatgaa gttactgaca gcccgatggt
     4501 ggataaatcc tggactggcc tgatttctac cgggatcacc tacaaattct gataatgcat
     4561 ctttccaggg cattttgtgc ataaaaacag ggcgtcacgc cctgttttgc attacggtgg
     4621 tgcaatacct gggaggaaac aatgcaacaa aaaatgattc aatttagtgg cgatgtctca
     4681 ctgccagcca tagggcaggg aacatggtat atgggcgaag atgccagtca gcgcaaaaca
     4741 gaagttgctg cactacgcgc gggcattgag ctcggtttaa ccctcattga taccgccgaa
     4801 atgtatgccg atggcggtgc cgaaaaggtg gttggggaag cattaaccgg tctgcgcgat
     4861 aacgtctttc tcgtctctaa agtctatccg tggaatgctg gcgggcaaaa agcgataaat
     4921 gcatgcgaag ccagtttacg ccgtctcaat actgattatc tcgatctcta cttattacac
     4981 tggtctggca gtttcgcttt tgaagagact gtggcagcga tggaaaaatt gatcgcccag
     5041 ggaaaaatcc gccgctgggg cgtttctaac cttgatcatg ctgatatgca ggaactctgg
     5101 cagctgccgg ggggaaatca gtgtgccact aatcaggtgc tttaccatct cggttcacga
     5161 gggattgagt acgatctact cccctggtgc cagcaacagc agatgccggt gatggcttac
     5221 agtccattag cccaggccgg gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac
     5281 gaaattgcac atgctcacaa tatcagcgcg gcacaagtat tgttggcgtg ggtaatcagc
     5341 catcagggtg tgatggcgat tccaaaagcg gccacgattg cccatgtcca acagaatgcg
     5401 gctgtgcttg aggtcgaact ttcgtcagcg gaattaacca tgctggataa agcgtatccg
     5461 gcaccaaaag gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg
     5521 cacccggcac acaacattaa cgcaaattaa cgtttcgcaa cgcgaatgga ttgcgccaga
     5581 tgtgcaggtt tctctttggt cactttttgc gtttctgaag cgtaagccgt ttctacacaa
     5641 acaaatgttt tgtagccatc atccggcatg tcgcccatgc taactgaaag cgccggtccc
     5701 gggttccagc cgacaacgtt cagatgatgc tggtggccta cggcgataat acgattcagc
     5761 gcttcatcat taatcacgct gcaatcttgc ggattcagat acacgcgatc ggtacggtca
     5821 gggaaggtct gaataccgtc ggtcagtaca tcttctttcg cgtcatttac tttatcaatg
     5881 aagcgatcgc ccagcccact gacgcttacc ttagcgatat cacccacgtt aaagtaggta
     5941 tgcagggcag aggtggtttc aaattcgcca tgtgattcaa gatcgatttc acaggtttta
     6001 cccacgcgga aatgcgctaa cagcgtaaag tcgtgcggcc agaatttttt cgtctcttcg
     6061 ctttgcgtca attcaaaagt cagcgctacg ccatcagcat cttcacgatg tgatttcagc
     6121 gtccacggca ggttgcgggc aaaaccgtgc gcaggcagac cttgttgtgc cgccggacca
     6181 aaccacggcc agcaaaccgg tacgccaccg cgaatagcga cgccattttt gaacggtgtg
     6241 ttgttgctca accacagaac ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg
     6301 ccctgtaatg caaaagaggc ttttacctgg ggatgatcga ccacaatgag gtccagttca
     6361 tccagtttac gacgggagag gacaggggag atttgttcga tgaccggaag ggcaaaaatt
     6421 ttcttaatca tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa gagcgaccga
     6481 agtcgctctt tttagatcac agtgtcatct caacttattt ggagatgtga gcgatcaggt
     6541 ccagaacttt gttggagtaa ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt
     6601 tgtcgttcag agcgatacca gctttagcat cgaacacgga agtgcaaact tcgccgttga
     6661 aatcggtaga tactacgtca tcttcggtgt agcccagaac gcctttcatt tcgccttcag
     6721 cagcagcttt aacggcagct ttgatctgct cgtaagttgc agctttttcc agacgaacgg
     6781 tcaggtcaac tacggatacg ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc
     6841 cattcagttc tggcagtact ttacctacag ctttagcagc accggtagag gacgggatga
     6901 tgttctggga agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca tcaacggttt
     6961 tctgagtagc ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat
     7021 cgttgataac tttagccagc ggagccaggc agttggtggt gcaggaagcg ttggaaacga
     7081 tgtcctggcc agcatatttg tcgaagttag cgcctttaac gaacatcgga gtgttgtctt
     7141 tagacggacc agtcataacc actttcttcg caccagcggt gatgtgttta cgagcagttt
     7201 cgtcagtcag gaacagacca gttgcttcag cgacaacgtc aacaccaact tcgtcccatt
     7261 tcaggttagc cggatcacgt tcagcggtaa cacggatttt tttaccgtta acgatcagat
     7321 gaccgtcttt cacttcaacg gtaccgtcga aacggccgtg agtggagtca tatttcagca
     7381 tgtatgccat gtaatcagcg tctaacaggt cgttgattgc aacgatctcg atgtcagaac
     7441 gtttctgagc agcacggaaa acaatgcgac cgatacggcc aaaaccgttg atacctactt
     7501 tgatagtcat atattccacc agctatttgt tagtgaataa aaggttgcct gtaaaattac
     7561 aaaaacctta cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg
     7621 cctaagcatt acgcgactga ctcgcctcac tcttcctttg ggcttgagac cacatggggt
     7681 cggcgccccg aattttaaag ggcaattacg ataaaaatgt gattttcatc acgatttagg
     7741 tgcagccatt tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt
     7801 tgttagaatc agtcacgtaa tgtgagcacg attaaagtga gatgtgagca aatggctaat
     7861 aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag
     7921 aatcatggga cagagccgcc atttacgggt cgtttactgc ataacaagcg tgacggcgta
     7981 tatcactgtt tgatctgcga tgccccgctg tttcattccc aaaccaagta tgattccggc
     8041 tgtggttggc ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac
     8101 ttgtcacatg gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg
     8161 catgtcttcc ctgacgggcc gcagccaacg ggcgaacgtt attgtgttaa ctctgcctct
     8221 ttacgcttta ccgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca
     8281 acttattcca caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc
     8341 ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct gatggcgttg
     8401 cgttgaccga ggaacaaaaa gagaattgcc tgcaactggt gatgctgtgg caagcccgcc
     8461 ataataccga agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca
     8521 aacagcagtt aaaagaagat tttggtatct cagcaaagcc tattgctatg tttaagtgaa
     8581 tgagttatct agtatttaag cctgttatta agggagggca aatatacttg ttaggacgtc
     8641 gtgttttagc tgcaagtagg ttaaatgcaa cactgacaag tattatctgg cctacaatgt
     8701 cattgaggaa atgtaggcca gagatgaatt caactaatat tacccctgcg tctcttccca
     8761 gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg cgtgcgcact
     8821 gtcctggggc tggatattca cgccacgaca accatcggta atcacgttta ctttataacc
     8881 taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca ggcccataac
     8941 gatcaattca tcgatttcat gatcgcgtaa ccagtcatcg agagaggttt tctgccgacg
     9001 gccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt tatggaacac
     9061 cgctgcgatc gctttttggt tcagtaacgg atgtaattgt gcgccttcac tgttctgcac
     9121 acagtgatct ggccagaagg tttgtggcaa accgtcgagt tgacctggca tataaggctc
     9181 tacacagtgc tgactggcaa aactgccgtg attcgccggg tgccagtcct gactggcgat
     9241 aaccgcttca ccgcgcgact ggcaccagtc aataaggcgg ttagcgacat ccaccgtact
     9301 gtcaccttcc ggcacggcga gcgcgccacc agcacagaaa tcattttgta aatcgaccag
     9361 taacagggcg cgagggggca ttgcagtctc cttaatcatc cggcgtcagt tcgccgcgca
     9421 ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc agcaggtaat
     9481 gcagtttggt tagcgtagct tctacagtca tatctgcacc gccaattacg ccggcatggg
     9541 cgagggcgtt accggtggca taaccaccca tgttcacttt accggacata cattgtgtca
     9601 ggttaaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg aaggctttgt
     9661 tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc ggttggcgca
     9721 gaaaattgcg cacgacatca gcagaaatcc ccggataaat cgtcactacg ccaattggtt
     9781 gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt ggcgtattca
     9841 aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag gcaaacgcat
     9901 caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg ttattgaaaa
     9961 agagcgttac ttcgttgatc ggataattcg ccgcaacgta caacgcattc agcagattaa
    10021 tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca atgaccggtt
    10081 taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc gtgtcggtac
    10141 cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct tcagcaatat
    10201 gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat tcatgaatgg
    10261 tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg cgttgtagat
    10321 gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc ccgcccgtgt
    10381 aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg aaagagcaaa
    10441 agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga aatatgggtc
    10501 gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga tctgtcggcc
    10561 actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata acggttttgt
    10621 gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc taccgaggca
    10681 agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac agaaccagac
    10741 atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc cagatgccac
    10801 tgtttcactt ttgccagctc tgctgctttg gcaaccgcat catcgaaatc cccgagacta
    10861 tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg ggcgatttta
    10921 tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa gcgtttatag
    10981 ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc cctggtgata
    11041 gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat cgaatccaga
    11101 ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag ggtgctgggg
    11161 ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc cgccatgccg
    11221 cccatcgata caaccacagg cttacccgct gcccgggctg ctgccagttc agcgcgaatc
    11281 acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat cgctttcact
    11341 ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc aacattcccc
    11401 tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat gctgtcaccg
    11461 gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata atttttatca
    11521 gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact cgatgccagt
    11581 gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt tttggttaaa
    11641 cccacaagca acccttgcgc gccagggaat acctgctgag cagggatcag ccggttagcg
    11701 gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc agcttcgcgc
    11761 gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata cgtacccacg
    11821 cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta cagaccgttg
    11881 gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt attggcgaaa
    11941 ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac cggtttcccg
    12001 ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg gtcgccgcct
    12061 gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt cgcctggcga
    12121 atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact ggcaccaagc
    12181 agctggcggc ttaatttact aaaccgctga gaactgtcgg gtttatcgac gatcacacca
    12241 gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc accactgacc
    12301 tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt aagtaccatt
    12361 tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat aaatcgccaa
    12421 agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca atagatgtgg
    12481 ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc tgtaacaaaa
    12541 tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca aatggatgca
    12601 ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc gccaacgggt
    12661 gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa gtccatgcaa
    12721 ccgtggcatt tttttgtgat tgaaggggaa gggcgcgagc gtttcagcgc cgtactggaa
    12781 cagggggcga ttgctgccgg tagtgatgac aaagctatcg acaaagcccg taatgcgccg
    12841 ttccgcgcac cgctcatcat cacggtggtg gcgaaatgcg aagaaaatca taaagtcccg
    12901 cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat ggcagccgtt
    12961 gcccaaggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc ggtagtgcgt
    13021 gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct cggcacgccg
    13081 cagttgaaag catctacgtc gattaacgtc ccggacccga ctccgtttgt aacttatttc
    13141 tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc agacactctc
    13201 acttatcact tcacggaatg agggttacca tacccggatt gcattgacag gagatgtcca
    13261 tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc ggctgtaaaa
    13321 tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt gttgatccga
    13381 atttgcttgt gggtaatgag acccgcgacg atgcggcggt gtacgatctg ggcaatggca
    13441 ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct ttcgattttg
    13501 gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc aaaccgatta
    13561 tggcgattgc gatcctcggc tggccgatta acaaactttc cccagaaatt gcccgcgaag
    13621 taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct ggcggtcact
    13681 ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta ccgaccgagc
    13741 gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg aaaccgctgg
    13801 ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa catcagggac
    13861 tggcgacgga agtgatgtgc cggatgaaca tcgcaggtgc gtcctttgcc aacatcgaag
    13921 gcgtaaaagc gatgaccgac gttactggtt ttggtctgct gggccatttg agcgaaatgt
    13981 gtcagggcgc aggtgtgcag gcgcgcgttg actatgacgc gatcccaaaa ttgccggggg
    14041 ttgaagagta cattaagttg ggcgcagtgc ctggcggcac cgaacgtaac tttgccagct
    14101 acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga tctgctgtgc gatccgcaaa
    14161 cttctggcgg tttgctgctg gcggtcatgc cggaagcaga aaatgaggtt aaaactacag
    14221 ccgccgagtt tggcattgaa ctgacggcga ttggcgaact ggtgccagcg cgcggcggtc
    14281 gtgccatggt tgagatccgt taattcaatg cggttgttta ttgccgaaaa accgagtctg
    14341 gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgta aaggcgatgg ctttatcgag
    14401 tgcggtaatg gtcaggtggt gacctggtgt atcggtcacc tgcttgagca ggcgcagcca
    14461 gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg atttgccgat tgtcccggaa
    14521 aagtggcaat tacagccacg accctccgtg accaaacaac ttaacgtcat caaacgcttc
    14581 ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg gcaattgctg
    14641 gtggatgaag tgctggacta tctgcaactg gcaccggaaa agcgccagca ggtgcagcgt
    14701 tgcttgataa acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg tcttcgttcc
    14761 aacagtgagt ttgtaccgct gtgcgtttct gcgctggcgc gagcgcgtgc cgactggctg
    14821 tacggcatca atatgacccg tgcttacacc attctcggtc gcaatgccgg ttatcagggc
    14881 gtgctttccg tcggacgcgt gcagacgccc gtgcttgggc tggtggtgcg ccgcgatgaa
    14941 gagattgaaa acttcgtggc gaaagacttc tttgaagtca aagcgcatat cgtgacacct
    15001 gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc gtaccaggat
    15061 gaagaagggc gcttgttaca tcgtccactg gcggagcatg tggttaaccg cattagtggt
    15121 caaccggcta ttgtcaccag ctataacgat aaacgggaat cagaatccgc gccgctgccg
    15181 ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct ttggtctgag cgcgcagaac
    15241 gtgcttgata tctgccagaa gctgtacgaa acacacaagt taatcactta tccgcgttcc
    15301 gattgtcgct atttgccaga agaacatttt gccggacgcc acgcggtgat gaatgccatc
    15361 agcgttcatg caccagatct gttgccgcag ccagtggtag atccagatat acgcaaccgc
    15421 tgttgggatg acaaaaaggt cgatgcgcac cacgccatca ttccgaccgc acggagttct
    15481 gcgatcaacc tgacggagaa cgaagcgaag gtctataacc tgattgcccg tcaatacctg
    15541 atgcagttct gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga cattgccaaa
    15601 ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag gctggcgtac gctgttaggc
    15661 agcaaagagc gcgatgaaga aaacgacggc acgccattgc ctgtggtggc gaaaggcgat
    15721 gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc gcgccatttt
    15781 accgatgcaa cactgctttc ggcgatgacc gggatcgcgc gctttgtgca ggacaaagat
    15841 ctgaaaaaga tccttcgtgc gaccgatggt ctggggacag aagcaacccg tgccgggatt
    15901 attgaactgc tgttcaagcg tggttttctg accaaaaaag ggcgttatat ccactccacc
    15961 gacgccggaa aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc ggacatgacc
    16021 gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta tcaggacttt
    16081 atgcagccgc tggtggggac gctatatcag cttattgatc aagccaaacg tacgccggtg
    16141 cggcagtttc gcggcattgt ggctccgggc agtggtggca gtgctgataa gaaaaaggct
    16201 gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag atgaagtcgg aagcggggcg
    16261 atagcgtaag cgagtgaatc tttcgtgcta ttcgagtcat attctgaaat atccagcgga
    16321 tcaagaaaat tcgttggata tttttttgca tggataaaat tatcgcctct aaagtatgta
    16381 ataacaggga atgtgatgaa aaaagtttta ttacaaaacc atcctgggag cgagaagtat
    16441 tcttttaatg gctgggaaat atttaatagt aattttgaac ggatgattaa agaaaataag
    16501 gccatgctgc tttgtaagtg gggggggtat ttaacatgtg ttgtcgctgt aatgtttgta
    16561 ttcgcagcga taacatccaa cggtttgaat gaaagaggcc tgattactgc gggatgctct
    16621 tttctttatc tattaattat gatggggctt attgttcggg ccggttttaa agcaaaaaaa
    16681 gaacaactgc attattatca ggctaaaggt attgagccgc tcagtatcga gaagttacag
    16741 gcgctacaat tgatcgcacc ttatcggttc tatcataagc aatggtctga aacgctggag
    16801 ttctggccgc gaaagcctga acctggcaaa gataccttcc aatatcatgt gcttcccttt
    16861 gactcgatcg atatcataag taaaagacgc gagtctttag aggatcaatg gggtatcgaa
    16921 gatagcgaaa gttattgtgc cttaatggag cattttcttt ctggcgacca tggagccaat
    16981 acctttaaag caaacatgga ggaagcccca gagcaggtta tcgccttgtt gaataaattt
    17041 gctgtttttc cctcagacta tatctctgat tgcgctaata atcgctccgg taaatcctcg
    17101 gcgaagctaa tatgggcggc ggaattatca tggatgatct cgatatcaag cacagctttt
    17161 caaaacggga caattgaaga agaactggcc tggcatgata taatgcttgc ttctcgaaag
    17221 gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat gggatttctt
    17281 tactggcata tctgctgcta tcgcagaaag ttaacggatg ctgaacttga agcgtgttat
    17341 cgttacgaca agcagttttg ggagcactac agtaagaaat gccgttggcc cataagaaat
    17401 gttccctggg gagcatcatc cgttaaatac tcataaacgc ctgaaatttt gccgggggcg
    17461 ctgcgcttgc ccggcctaca ttagagcgta actgcaatga attgtgccca t
//
LOCUS       DAPPNJ010000092        17481 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000092 DAPPNJ010000000
VERSION     DAPPNJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.092"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            217..1269
                     /locus_tag="RPM73_002535"
     CDS             217..1269
                     /locus_tag="RPM73_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDX6520882.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1585..2787)
                     /locus_tag="RPM73_002536"
     CDS             complement(1585..2787)
                     /locus_tag="RPM73_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDX6520883.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3019..4518
                     /gene="pitA"
                     /locus_tag="RPM73_002537"
     CDS             3019..4518
                     /gene="pitA"
                     /locus_tag="RPM73_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDX6520884.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4589..4924)
                     /gene="uspB"
                     /locus_tag="RPM73_002538"
     CDS             complement(4589..4924)
                     /gene="uspB"
                     /locus_tag="RPM73_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDX6520885.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5315..5749
                     /gene="uspA"
                     /locus_tag="RPM73_002539"
     CDS             5315..5749
                     /gene="uspA"
                     /locus_tag="RPM73_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDX6520886.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            6067..7536
                     /gene="dtpB"
                     /locus_tag="RPM73_002540"
     CDS             6067..7536
                     /gene="dtpB"
                     /locus_tag="RPM73_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDX6520887.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7585..8337)
                     /gene="rsmJ"
                     /locus_tag="RPM73_002541"
     CDS             complement(7585..8337)
                     /gene="rsmJ"
                     /locus_tag="RPM73_002541"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDX6520888.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8345..10387)
                     /gene="prlC"
                     /locus_tag="RPM73_002542"
     CDS             complement(8345..10387)
                     /gene="prlC"
                     /locus_tag="RPM73_002542"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDX6520889.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10590..11432
                     /gene="rlmJ"
                     /locus_tag="RPM73_002543"
     CDS             10590..11432
                     /gene="rlmJ"
                     /locus_tag="RPM73_002543"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDX6520890.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11504..12856
                     /gene="gorA"
                     /locus_tag="RPM73_002544"
     CDS             11504..12856
                     /gene="gorA"
                     /locus_tag="RPM73_002544"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDX6520891.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12910..12993)
                     /locus_tag="RPM73_002545"
     CDS             complement(12910..12993)
                     /locus_tag="RPM73_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDX6520892.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13502..13855
                     /gene="arsR"
                     /locus_tag="RPM73_002546"
     CDS             13502..13855
                     /gene="arsR"
                     /locus_tag="RPM73_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDX6520893.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            13909..15198
                     /gene="arsB"
                     /locus_tag="RPM73_002547"
     CDS             13909..15198
                     /gene="arsB"
                     /locus_tag="RPM73_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDX6520894.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15211..15636
                     /gene="arsC"
                     /locus_tag="RPM73_002548"
     CDS             15211..15636
                     /gene="arsC"
                     /locus_tag="RPM73_002548"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDX6520895.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALMRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15759..16055)
                     /locus_tag="RPM73_002549"
     CDS             complement(15759..16055)
                     /locus_tag="RPM73_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6520896.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16163..17176
                     /locus_tag="RPM73_002550"
     CDS             16163..17176
                     /locus_tag="RPM73_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX6520897.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
BASE COUNT         4039 a         4487 c         4403 g         4552 t
ORIGIN      
        1 taattgtaaa aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg
       61 gccttttaac cggtaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg
      121 catttgtctt taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac
      181 atttcattat tagcagggat acctgaggag tttaaagtga acatatatat cgggtggctt
      241 ttcaaattaa tccctttgat tatgggatta atttgcatcg cgttaggtgg ctttgtgctg
      301 gaaagttcag ggcaaagcga gtatttcgtc gcgggtcatg tgctgatttc tctggcggcc
      361 atatgcctgg cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt
      421 aatacgtttt acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg
      481 atatggggtt gggcactgtt agcaggcaat gatgtgatgg cagacgagtt tgtcgccggc
      541 catgttattt tcggcgttgg tatgattgcc gcctgtgtat cgacggtggc agcgtcatcc
      601 ggtcactttc tgctcattcc caaaaatgca gcggggagca agagcgatgg aacgccggta
      661 caggcttatt cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg
      721 ctcggtttca tttggtctat tacgctgtta cgtagtgctg acataactcc gcattatgtc
      781 gcgggtcacg tattgcttgg gttaaccgca atctgtgcct gtctaattgg ccttgttgcc
      841 acaattgtcc atcaaacacg taatacgttt tcaactaaag aacactggct gtggtgttat
      901 tgggttattt ttctcggctc aatcacggta ctgcagggga tatacgtctt agtcagttcc
      961 gatgcaagcg cccgactggc tcccggcatt attcttattt gcctcggaat gatctgttac
     1021 agcatattct caaaagtctg gctactggca ctggtatgga gacgtacctg ttcgttagcc
     1081 aacagaatac cgatgattcc cgtcttcacc tgcctgtttt gccttttcct ggcatcgttt
     1141 cttgcggaaa tggcgcagac cgacatggga tattttattc cttcgcgggt tttggtcggt
     1201 ttaggagcgg tatgctttac gttgttctca atcgtttcaa tattagaagc aggttctgct
     1261 aaaaaataat tgcaacgtac cggataaaac cagcgttgac catttgcgta acgctggttt
     1321 ttcttaggca tcatgaaata acgcagcatt aatgcatagt ggttaaggat aaaaaaacaa
     1381 caaagttact gtttttttca acctgttcat attttataaa gatctggcca ctgggtgagt
     1441 tttcaatcac ctttccttcc accttatatt aagcctggag ggtttcagtt cacgggctca
     1501 ttagaaaata atcacaagat agccactatc aaaattagct catttttaat gcggctctaa
     1561 taattttcat ctttaggtaa caggtcagga cgacttcgct gcaatcaaat cctgcgcaca
     1621 agcccacgca ctcgaccacg cccactggaa gttatagccc cccagccagc cggtaacgtc
     1681 catcacttcg ccgatgaagt acagcccagg cactttgcgc gcttccatcg tccgtgaaga
     1741 gagttcgttg gtgtccacgc cgccgagcgt cacttcggca gtgcgatagc cttcagtgcc
     1801 gttgggttgt acgcgccagt cggtcaatgt gctaatcagt gcctgttggt cacgcacgtt
     1861 gagctgtttt agcgaaacat ccgggatttg cccgagttgc tgtaagcgtt caaccaaccg
     1921 cttcggtaga tgaaccgcca gtgtgttttt caggctctga ttcggatgtg cgttacgctg
     1981 ctcattcagg aaggtttcga gatccacatc cggtagcaga ttgatactga caaattcacc
     2041 cggttgccag tagctggaaa tctgcaacac cgccggtcca gacaagccgc gatgggtgaa
     2101 gagtaagttc tcacggaaaa cgatgccgtt ttcagcggta atcacggaag gcaccgccac
     2161 gcccgccagc acctgtaact cttcgagcaa cggtttatgc agagtgaatg gcaccagacc
     2221 cgcgcgggtc ggcagcacgt tgaggccaaa ttgttcggca atcttataac caaacggcga
     2281 cgcgcccagc cccggcattg acagcccacc ggtcgcgatg accagctttt cgcaaccgac
     2341 agtcatgccg ttcagttcaa gcgtgaagcc tgtttcatcc ttcgccacac tcagcacttc
     2401 gctacgcaat ctgaaggtta cattgccctt ctcgcactca tccaccagca tgtcgacaat
     2461 ctgctgcgcg gagtcatcgc agaagagttg tcctaacgtt ttctcgtgcc aggcgatgcc
     2521 gtgtttattg accagatcaa tgaaatccca ctgggtaaaa cgcgcgagtg cagacttaca
     2581 aaaatgcgga ttctggctca gataagcgcc tggttcgaca taaaggttgg taaagttgca
     2641 gcgcccaccg ccagacataa ggattttgcg ccctggtttt ttaccattat cgatcagcag
     2701 aacccggcgt cctgcctgac ctgccagcgc agaacagaac atacccgccg caccagcgcc
     2761 tataataatg gcatcaaacc tttccacgtt gcgctcctct tagaaaaaac gggcgtgaat
     2821 tgtaaagatt cctcagtggt cgcaccagca tcaatattac taaaaggaag tatttgcctg
     2881 aattatataa gataattatt ttttgagtga aatccataca ggaggcaaat caaaaaaagt
     2941 ctatatttca ctttgcccgc gccgcgaaag tcactgataa tgcgccgcgt tcatgtcctc
     3001 aaaatggcgt aacgtcctat gctacatttg tttgctggcc tggatttgca taccgggctg
     3061 ttattattgc ttgcactggc ttttgtgctg ttctacgaag ccatcaatgg tttccatgac
     3121 acagccaacg ccgtggcaac cgttatctat acccgcgcga tgcgttctca gctcgccgtg
     3181 gttatggcgg cggtgttcaa ctttttgggt gttttgctgg gtggtctgag tgttgcctat
     3241 gccattgtgc atatgctgcc gacggatctg ctgcttaata tgggatcgtc tcatggcctt
     3301 gccatggtgt tctctatgtt gctggcggcg attatctgga acctgggtac ctggtacttt
     3361 ggtttacctg catccagctc tcatacgctg attggcgcga tcatcgggat tggtttaacc
     3421 aatgcgttga tgaccgggac gtcagtggtg gatgcactca atatcccgaa agtattaagt
     3481 attttcggtt ctctgatcgt ttcccctatt gtcggcctgg tgtttgctgg cggtctgatt
     3541 ttcttgctgc gtcgctactg gagcggcacc aagaaacgcg cccgtatcca cctgacccca
     3601 gcggagcgtg aaaagaaaga cggcaagaaa aagccgccgt tctggacgcg tatcgcgctg
     3661 atcctttccg ctatcggcgt ggcgttttcg cacggcgcga acgatggtca gaaaggcatt
     3721 ggtctggtta tgttggtatt gattggcgtc gcgccagcag gcttcgtggt gaacatgaat
     3781 gccactggct acgaaatcac ccgtacccgt gatgccatca acaacgtcga agcttacttt
     3841 gagcagcatc ctgcgctgct caaacaggct accggtgctg atcagttagt accggctccg
     3901 gaagctggcg caacgcaacc tgcggagttc cactgccatc cgtcgaatac cattaacgcg
     3961 ctcaaccgcc tgaaaggtat gttgaccacc gatgtggaaa gctacgacaa gctgtcgctt
     4021 gatcaacgta gccagatgcg ccgcattatg ctgtgcgttt ctgacactat cgacaaagtg
     4081 gtgaagatgc ctggcgtgag tgctgacgat cagcgcctgt tgaagaaact gaagtccgac
     4141 atgcttagca ccatcgagta tgcaccggtg tggatcatca tggcggtcgc gctggcgtta
     4201 ggtatcggta cgatgattgg ctggcgccgt gtggcaacga ctatcggtga gaaaatcggt
     4261 aagaaaggca tgacctacgc tcaggggatg tctgcccaga tgacggcggc agtgtctatc
     4321 ggcctggcga gttataccgg gatgccggtt tccactactc acgtactctc ctcttctgtc
     4381 gcggggacga tggtggtaga tggtggcggc ttacagcgta aaaccgtgac cagcattctg
     4441 atggcctggg tgtttaccct tccggctgcg gtactgcttt ccggcgggct gtactggctc
     4501 tccttgcagt tcctgtaatc gtacacacca aaacgagcgg gtcagtttac tggcccgctt
     4561 tttttatgcg ttattattgc tgaagtgctt aatgccaaat catcaatgca atcaggctga
     4621 ccaccaccag accacacaat gcgctggtca gaataaactg ccgacgcacc cgctcacagc
     4681 ggcgaataaa ttcatcatcg tgatgatcgc gataacgttg ggcatagata taccaaacga
     4741 gacgcacctg tttgttgggt tggccatgtg aggtaaaaaa gccccctcca tcaacatatt
     4801 gatagagcaa tggatcgcag ttacgcagta ccactaacaa cgcgcgtagt gatgagaaat
     4861 agcgcgccat gttaacaatg caaacgacac ataaagccca aaataatgcg acggtgctta
     4921 tcatacctcc tccccggcga cctgcccgcg gagttccacc ccggggctac cgctcccgat
     4981 acgctgccaa tcagttaaca ccaggtcctg gagaaaccgc ttttgtggtg accaacatcc
     5041 gagcggctct atagatagtg taggagatca ggttgttttt tttccagaag gttaaccact
     5101 atcaatatat tcatgtcgaa aatttgttta tctaacgggt aagcaaggcg gattgacgga
     5161 tcatccgggt cgctataagg taaggatggt cttaacactg aatctttacg gctgggttag
     5221 ccccgcgcac gtagttcgca ggacgcgggt gacgtaacgg cacaagaaac gctagctggc
     5281 cagtcatcga caactttatg gaaggagtaa cactatggct tataaacaca ttctcatcgc
     5341 agtcgatctc tccccggaaa gcaaagttct ggtagagaaa gcagtctcta tggctcgccc
     5401 ctacaatgcg aaagtttctc tgatccacgt agatgtaaac tactctgacc tatacaccgg
     5461 gcttattgat gtgaatctgg gtgatatgca gaaacgcatc tctgaagaga cacatcatgc
     5521 gctgaccgag ctttccacta atgcaggcta cccaatcact gaaaccctga gcggcagcgg
     5581 cgacctgggc caggttctgg tcgatgcaat caagaaatac gatatggatc tggtggtttg
     5641 tggtcaccac caggacttct ggagcaaact gatgtcttcc gcacgtcagc tgatcaacac
     5701 cgttcacgtt gatatgctga ttgttccgct gcgcgacgaa gaagaataat catccctcca
     5761 cgacgtgttc ctgaacgccc gcatatgcgg gcgttttgct ttttggcgcg ccttgttacc
     5821 tgatgcagcg taaacccctt atctggccta cgatctgcgt acgcaatcaa aatctcctgc
     5881 caatacaaca tttaacacca tcatattttc catcattagt gtgatcagct ggttattttc
     5941 tgttgtaata gtgtattaat ctattcaccg catcaatatt aagaatctct gacagatgta
     6001 aactttttcg cgcgttatcc cttacgcgtt catacttttc aggatggtat tggaaggtta
     6061 ataaatatga atacaacaac acccatgggg atgctgcagc aacctcgccc atttttcatg
     6121 atcttttttg tcgagttatg ggagcgattc ggctactacg gcgtgcaggg cgtactggcg
     6181 gttttcttcg ttaaacagct tggattctcg caagagcagg cttttgtcac ttttggtgct
     6241 tttgctgcgc tggtctatgg cctcatttcc attggcggct atgtcggcga ccacctgctg
     6301 gggaccaaac gcaccattgt tctcggagca cttgtgctgg cgattggcta cttcatgacc
     6361 ggcatgtcgc tacttaagcc tgacctgatt ttcatcgccc tggggactat cgctgtcggt
     6421 aacggcctgt ttaaagctaa cccagccagc ttgctttcga agtgctatcc gccgaaagat
     6481 ccgcggcttg atggcgcatt caccctgttc tatatgtcga tcaatatcgg ctcgttgata
     6541 gcgttatcgc tggcccctgt gatcgctgat agattcggtt attcagtcac ctacaacctg
     6601 tgcggggcgg ggttaattat cgcattactg gtttacatcg cctgtcgtgg aatggtgaaa
     6661 gacattggtt ctgaacccga cttcaagccg atgagcttca gcaaactgtt gtacgtatta
     6721 cttggcagcg tggtgatgat cttcgtatgt gcatggctga tgcacaacgt agaagtcgcc
     6781 aatctggtgc tgattgttct ctccatcgtc gtcaccatca tcttctttcg tcaggcattc
     6841 aagctggata aaaccgggcg caataaaatg tttgtcgcct ttgtcctgat gctcgaagcg
     6901 gtggtgtttt acattctcta cgcccagatg ccaacatcgc tgaacttctt tgccatcaac
     6961 aacgtgcatc atgaaattct cggtttttcc atcaacccgg tcagcttcca ggcgcttaac
     7021 ccgttctggg tggtactcgc cagcccaata ctggcaggca tttacacgca tctgggtaac
     7081 aaaggcaaag acctctcgat gccgatgaaa tttactctcg gcatgtttat gtgctcactg
     7141 ggctttttga cggcggcagc tgcgggaatg tggtttgcgg atgcacaagg gctgacatcg
     7201 ccatggttta tcgtgctggt gtacttattc cagagcttag gtgaactgtt tattagcgcc
     7261 cttggcctgg cgatgattgc tgccctggtg ccgcagcatt tgatgggctt tattctcggg
     7321 atgtggttcc tgacgcaggc tgccgcgttc ttgctgggcg gctatgtggc aacatttacc
     7381 gcggtgccgg acaacattac cgatccgctt gagacgttgc ccgtctatac caacgtgttt
     7441 ggtaagattg gtctggtcac gctgggcgtt gcagtagtga tgctgttgat ggtgccgtgg
     7501 ctgaaacgca tgattgcgac gccggaaagc cattaattat tcttgcagaa agcagggtag
     7561 cgttatcgct accctgcttt acttttacac gggcgtacct gcatagatat caaagcgatg
     7621 ccctttagtc acaaccgcgt ttggcgtggc gacattcgct agcggtggcg cgtagtccgg
     7681 gcgctttact accacgcgtt tggtcgccag caagcgtgca ggctccagca atccatcggc
     7741 atcaagatcc ggccccacca gcgactgaaa gacacgcatc tcttttttca ccagcgcgct
     7801 tttctgctta tgcgggaaca tcgggtcgag atagaccacc tgcgggcgtg gggtaatatc
     7861 ggttagcgcc gtcaggctgg aggcgtgaat taactgcaaa cgctcctgca accagccgcc
     7921 gatttccgca tccgcataac cacgcgccag gccgtcgtcg agcaatgcgg caacgactgg
     7981 attacgctcc agcatccgca cgcggcaacc gactgaagcc agcacaaagg catcgcgccc
     8041 cagccctgcg gtggcgtcca ccacatccgg caaataatcg cctttaatgc ccaccgcttt
     8101 cgccaccgcc tcaccgcgac caccgccgaa tttgcgtcgg tgcgccatcg ctccgccaac
     8161 aaaatcaaca aatatgccgc caagttttgg ctcatcacgc ttgcgcaatt ccagatgttc
     8221 cggcgttaac accagcgcca tcaggttgtc ttcatcgtgc tccagccccc agcggaccgc
     8281 cagaacagat aaggcaccgt ctccggtgcc tgtttcatca attaagcaga ttttcactga
     8341 atgatcagcc cttaatgccg taatgctcca gcatcgcatc cagctgcggt tcacgaccac
     8401 ggaagcgttt gaacagatcc atcggctctt ctgaaccgcc acggctcaga atgttgtcga
     8461 ggaacgactg cccggtttca cggttgaaaa tgccctcttc ctcaaagcgc gagaaagcat
     8521 ctgccgccag tacgtcagcc cacaggtagc tgtagtaacc tgcggcataa ccaccggcga
     8581 aaatatggct gaaagcgtgc gggaaacggc cccaggatgg agacggtact acggcaacca
     8641 gtttcttgat ttctgccaga gtttcgagga ttttcgctcc ctgatccggg cggaactcgg
     8701 catggaggcg gaaatcgaac aggccgaact ccagctggcg cagaataaac agcgccgcct
     8761 ggtagttctt cgccgccagc attttatcca gcaactcttt cggcagcggt tcgccggttt
     8821 cgtagtgacc ggagataaac gccagcgcct ccggctccca gcaccagttt tccataaact
     8881 gactcggcag ttcgaccgca tcccacggca caccgctgat accggaaaca ccagcggttt
     8941 cgatgcgggt cagcatatgg tgcaggccgt gaccgaactc gtggaacagg gtgatcactt
     9001 cgtcgtgagt gaacagcgcc ggtttaccat ttaccgggcg gttgaagtta caagtcaaat
     9061 acgcgaccgg tttttgcaga gaaccatcag ctttacgcat ctggcctacg cagtcatcca
     9121 tccacgcccc gccgcgcttg ttttcacggg catacagatc gaggtagaag ctgccgcgca
     9181 gttcgttatt ttcgtcatac agttcgaaga aacgtacatc cggatgccag acatcaacat
     9241 ctttacgctc tttagcggtg atgccgtaaa tacgtttcac cacttcaaac aggccgttaa
     9301 ccgctttgtt ttccgggaag tacggacgca gctgttcgtc actgatgctg tagaggtgct
     9361 gtttttgttt ttcgctgtag tacgcgatat cccacggctg caactcatcg acgccaaatt
     9421 ccgctttggc gaaggcgcgc agttgtgcca gctctttttc gccttgcgga cgcgcgcgtt
     9481 ttgccagatc ggttaagaaa tccagcacct gctgcgggtt ttctgccatt ttagtggcaa
     9541 gggatttaaa ggcgtagttt tcaaagccca gcagttgcgc cagttcgtga cgcagagcga
     9601 ggatctcttc catcaccttg ctgttatccc atttaccggc gttcgggcct tgatcggagg
     9661 cgcgggtgct gtaagcgcga tacatctctt cacgcaaggc ctggttgtcg cagtaggtca
     9721 ttaccggcag gtagcttggg atatccagcg tcagcaggta gccttccagc tctttcgctt
     9781 cggcctgggc ttttgccgca gccagcgcgc tttcaggcat ccccgccagc tccgcttcgt
     9841 cggtaacgag tttggtccag cccatcgtcg catcgaggac gttgttgctg tactggttgc
     9901 ccagctcgga aagacgagtg gcaatctcac cgtagcgttg ttgtttctct attggcagac
     9961 cgatgccaga gagttcgaag tcgcgcagtg cgttatcaac cgcttttttc tgagccgtgt
    10021 tcagcgtggc gtaatgatcg ccatcgcgca ggtcgcgata cgctttatac agcccttcat
    10081 gttgccctac ccaggtgctg tattccgaca gcagcggcag ggtttgttcg tacgcttcac
    10141 gcagttccgg gctatttttc accgagttca ggtggctgac cggggagaag atacgcccca
    10201 gcacatcgtc cacttccgcc aacggctggc agagattttc ccaggtgtac ggtgctcctt
    10261 gcgctactac gcgctccaca ttttcgcggc agtcgttcag cgccttagtc acggctggaa
    10321 cgacatgttc cgggagaatt ttagaaaacg gaggcaattc aaagggagtc agtaacggat
    10381 tcgtcattcg cgcagtcctg gttaaagagg ttaaggaagc gctcaacagg cgctttacat
    10441 aatgtgtgta gcatggggtt aagtgtagtg aatttcaatg agaaacgtta cgctttcgcg
    10501 gcggcggcct cttttcggta tactgtcctg atacgctttt gtgcgccccg aatacgggcc
    10561 gatttttact taccggaaca cctttaccca tgctcagtta tcgccacagc tttcacgctg
    10621 gcaaccacgc cgacgtcctt aaacataccg ttcagagcct gatcatcgag tcgctgaaag
    10681 agaaagataa accgtttctc tatctcgaca cccacgcagg ggccgggcgt tatcagttag
    10741 gcagcgaaca tgccgagcgt accggcgaat atctcgaagg catcgcccgt atctggcagc
    10801 aggacgattt gcccgcagaa ctggaggcgt acatcaatgt ggtaaaacac ttcaaccgta
    10861 gcggtcagtt gcgttactac cccggttcgc cattgattgc tcgccagcta ctgcgcgaac
    10921 aagacagcct gcaactgacc gaactgcacc cgagcgatta cccgttgctg cgttctgaat
    10981 ttcagaaaga cagccgtgcg cgcgtcgaaa aagccgatgg tttccagcag cttaaggcca
    11041 aactgccgcc ggtttcccgc cgtggtttaa tccttatcga cccgccgtat gaaatgaaaa
    11101 ccgactatca ggcggtggtc agcgggatag cagaaggtta caaacgtttc gccactggta
    11161 cttacgcact gtggtatccg gtggtgctgc gtcagcaaat taagcgcatg atccacgatc
    11221 tggaagcgac cggtattcgc aaaattctgc aaattgaact ggcggtactg ccagacagcg
    11281 atcgccgtgg catgaccgct tccggcatga ttgtgattaa cccgccgtgg aaactggaac
    11341 agcagatgaa taacgtgctg ccgtggctgc acagcaaact ggttccggca ggcaccgggc
    11401 acgccaccgt aagctggatc gtgccggagt aattgcagcc attgctggca cctattacgt
    11461 ctcgcgctac aatcgcggta atcaacgata aggacacttt gtcatgacta aacactatga
    11521 ttacatcgcc atcggcggcg gcagcggcgg tatcgcctcc atcaaccgcg cggctatgta
    11581 cggccagaaa tgtgcgctga ttgaagccaa agagctgggc ggcacctgcg taaatgttgg
    11641 ctgtgtgccg aaaaaagtga tgtggcacgc ggcgcaaatc cgtgaagcga tccatatgta
    11701 cggcccggat tatggttttg ataccactat caataaattc aactgggaaa cgttgatcgc
    11761 cagccgtacc gcctatatcg accgtattca tacttcctat gaaaacgtgc tcggtaaaaa
    11821 taacgttgat gtaatcaaag gctttgcccg cttcgttgat gccaaaacgc tggaggtaaa
    11881 cggcgaaacc atcacggccg atcatattct gatcgccaca ggcggtcgtc cgagccaccc
    11941 ggatattccg ggcgtggaat acggtattga ttctgatggc ttcttcgccc ttcctgcttt
    12001 gccagagcgc gtggcggttg ttggcgcggg ttacatcgcc gttgagctgg cgggcgtgat
    12061 taacggcctc ggcgcgaaaa cgcatctgtt tgtgcgtaaa catgcgccgc tgcgcagctt
    12121 cgacccgatg atttccgaaa cgctggtcga agtgatgaac gccgaaggcc cgcagctgca
    12181 caccaacgcc atcccgaaag cggtagtgaa aaatgccgat ggtagcctga cgctggagct
    12241 ggaagatggt cgcagtgaaa cggtggattg cctgatttgg gcgattggtc gcgagcctgc
    12301 caatgacaac atcaacctgg aagccgctgg cgttaaaact aacgaaaaag gctatatcgt
    12361 cgtcgataaa tatcaaaaca ccaatattga aggtatttac gcggtgggcg ataacacggg
    12421 tgcagtggag ctgacaccgg tggcagttgc agcgggtcgc cgtctctctg aacgcctgtt
    12481 taataacaag ccggatgagc atctggatta cagcaacatt ccgaccgtgg tcttcagcca
    12541 tccgccgatt ggtactgttg gtttaacgga accgcaggcg cgcgagcagt atggcgacga
    12601 tcaggtgaaa gtgtataaat cctctttcac cgcgatgtat accgccgtca ccactcaccg
    12661 ccagccgtgc cgcatgaagc tggtgtgcgt tggatcggaa gagaagattg tcggtattca
    12721 cggcattggc tttggtatgg acgaaatgtt gcagggcttc gcggtggcgc tgaagatggg
    12781 tgcaaccaaa aaagacttcg acaataccgt cgccattcac ccaacggcgg cagaagagtt
    12841 cgtgacaatg cgttaaatgt taaagggcta agagtagtgt gctcttagcc cttaattacg
    12901 tttccgctat cagttcagaa gctgaagcag aaagcggatc agttccagca gcgcaattaa
    12961 cgccccaaga acgatgattg ctttatcaat cacccgtttt ctccatgcga tggagtgaga
    13021 atgcatccgc ttactcatcc actgcctgtc acggcgcatg ccctattgtt agataatggc
    13081 attctcactc cggccagagc atcagttaac ggcaccaccc gtacttctga ccaggacttt
    13141 gaaagcgttt atgcgcatta ccagagtgaa aatgcctcag agctaactgg ataatcatac
    13201 agtacattca ggttataaaa ccagcatgtc cttgcaatag tttcagtatg gtattagcat
    13261 tgatgcgtta gataatggca ttctcactcc agccagtgcc accaactcag ggctggaaag
    13321 taaaaaaccg acgcaaagtc ggttttttta cgtcctgatt cagacctcct ttcaaatgaa
    13381 tagccaactc aaaattcaca cctattacct tcctctgcac ttacacattc gttaagtcat
    13441 atatgttttt gacttatccg cttcgaagag agacactacc tgcaacaatc aggagcgcaa
    13501 tatgtcattt ctgttaccca tccaattgtt caaaattctt gctgatgaaa cccgtctggg
    13561 catcgtttta ctgctcagcg aactgggaga gttatgcgtc tgcgatctct gcactgctct
    13621 cgaccagtcg cagcccaaga tctcccgcca cctggcattg ctgcgtgaaa gcgggctatt
    13681 gctggaccgc aagcaaggta agtgggttca ttaccgctta tcaccgcata ttccagcatg
    13741 ggcggcgaaa attattgatg aggcctggcg atgtgaacag gaaaaggttc aggcgattgt
    13801 ccgcaacctg gctcgacaaa actgttccgg ggacagtaag aacatttgca gttaaaaatt
    13861 tagctaaaca catatgaatt ttcagatgtg ttttatccgg gaggcattat gttactggca
    13921 ggcgctatct ttgtcctgac catcgtattg gttatctggc agccgaaagg tttaggcatc
    13981 ggctggagtg caacgctcgg cgcagtactg gcgttagtta cgggcgtggt ccatccgggt
    14041 gatattccgg tggtgtggaa tatcgtctgg aacgcgacgg ctgcgtttat cgccgtcatt
    14101 atcatcagcc tgctgctgga tgagtccggc ttttttgaat gggcggcgct gcacgtctca
    14161 cgctggggta atggtcgtgg tcgcttgctg tttacctgga ttgtcctgct cggtgctgcc
    14221 gttgccgccc tgtttgccaa tgatggcgcg gcgcttattt tgacaccgat tgtcatcgcc
    14281 atgctgctgg ctttagggtt cagtaaaggc actacgctgg cgttcgtgat ggcggccgga
    14341 ttcattgccg ataccgccag cctgccgctt attgtctcca acctggtgaa tatcgtttcc
    14401 gctgatttct ttggcctcgg ctttcgcgaa tacgcctcgg tgatggtgcc ggtggatatc
    14461 gccgcgattg ttgccacgct ggtgatgtta catctctatt ttcgcaaaga tattccgcag
    14521 aactacgata tggcgctgct gaaatctccc gcagaagcga tcaaagatcc tgctacgttc
    14581 aaaactggct gggttgtttt actgcttctg ctggtgggat ttttcgtcct ggaaccgctc
    14641 ggcattccgg tgagcgccat tgcagctgtg ggcgcgctga tattatttgt cgtcgctaaa
    14701 cgcggtcatg cgattaatac gggtaaagtc ctgcgcggtg ccccctggca gattgtcatc
    14761 ttctcgctcg gcatgtatct ggtggtttat ggcctgcgca atgccggatt aacggaatat
    14821 ctttctggcg tactcaacgt gctggcggat aacggcctgt gggccgcgac gctcggcacc
    14881 ggattcctca ccgccttcct ctcttctatt atgaacaata tgccgacggt actggttggc
    14941 gcgttgtcca ttgatggcag cacggcatct ggcgttatca aagaagcgat ggtttatgcc
    15001 aatgtgattg gctgcgattt gggaccgaaa attaccccaa ttggtagcct ggctacgcta
    15061 ctctggctgc acgtactttc gcagaagaat atgactatca gctggggata ttacttccgt
    15121 acagggatta tcatgaccct gcctgtgctg tttgtgacgc tggctgcgct ggcgctacgt
    15181 ctctctttca ctttgtaatg agatactgat atgagcaaca ttaccattta tcacaacccg
    15241 gcctgcggca cgtcgcgtaa tacgctggag atgatccgca acagcggcac agaaccgacc
    15301 attatccatt atctggaaac tccgccaacg cgcgatgaac tggtcaaact cattgccgat
    15361 atggggattt ccgtacgcgc gctgatgcgt aaaaacgtcg aaccgtatga ggagctgggc
    15421 cttgcggaag ataaatttac tgacgatcgg ttaatcgact ttatgcttca gcacccgatt
    15481 ctgattaatc gcccgattgt ggtgacgccg ctgggaactc gcctgtgccg cccttcagaa
    15541 gtggtgctgg aaattctgcc agatgcgcaa aaaggcgcat tctccaagga agatggcgag
    15601 aaagtggttg atgaagcggg taagcgcctg aaataaagcg gcgatatcca ctgcccatag
    15661 ttggtaaagt gcgctttgtt tatgtcggat gcgacgctaa cgcgtcttat ccgacctaca
    15721 aaaccgtgca tggcatgaaa tctgtagcgc gtagcgcatc aggcaatttt gctatttacg
    15781 cagcactcct cgcgtaagaa ttcaattatc gcttccagca ttgcgtattg cgaaacacac
    15841 atcagcgtcc gactctcacg gttttgttta accagcccta ccgataccag tgctgaaata
    15901 tggtgactga gtgtagagcc gggtatccca agctgctttt gcaactcacc gaccggcaaa
    15961 ccttgctccc ccgctttgac caggtgtttg aatatgaaca aacgggtcgg gtgacctagc
    16021 tctttcagcg ctttagcaac ttcttccaac tgcatatgac accccctttg tctggttgat
    16081 atttcgataa taccagaaat atcttgatct gaatttcatt tcgattattc tggaaatata
    16141 gatttatcca atgaggttac gtatgagtag ttggcttgcg atgctgcaag atgccgcaga
    16201 gatgtttgtg tttctcgccg tcgagctttc gttgctgttt atagtgatta gtgccggtgt
    16261 cagcctgata agacaaaagg tgccagacca taaaatccag cagatgatgg gggccagaaa
    16321 agggagaggc tatctcctgg ctgctctgtt gggagccgtt accccgttct gtagttgctc
    16381 gacaataccc atgctacgtg gattgttgtc agcgaaagcc gggtttggtc cgactctcac
    16441 ttttttattt gtttcaccat tacttaatcc cattatcgtc ggactaatgt gggtgacctt
    16501 tggttggaaa gtcaccttgt tgtacgcgat tatcgccacc ggcgtctccg ttcttgccag
    16561 tattatcctg gattctctgg ggtttgaacg tcatatcatt gccagtaaaa gctcatcagc
    16621 aaattgttgt gctccagcca aagcgtcggc aaaaactttg ccggggacga catatacgcc
    16681 aatagaagtg agttgctgta gtccaacggc taaagccatt gagaaacccg tcgttaactg
    16741 ttgcaatacc aaagcagtgg caagtattaa tcccataaaa ctagccacca aagatgcgtt
    16801 gcaacagttt aaagatgtac tgccatatct tttgttaggg gtattaatag gctcttttat
    16861 ttatggcttt attccctcag cgtggattgc cgctcatgca ggagcagata atcccttcgc
    16921 catcccatta agcgccgttg tcggtattcc gctgtatatc cgggctgagg cggttatccc
    16981 tctggcatct gttctgatga caaaaggaat gggtctggga gcattaatgg cattaatcat
    17041 cggcagtgcc ggcgcaagcc tgacggaagt gatattactt aaatcaatgt tcagaatgcc
    17101 gatgatagct gcattcctga cggttatatt aggtatggct atcttgatgg gctatttgac
    17161 tcaattgcta ttttaaatag atgccagcag taaactataa gatgcatagt tagtgccata
    17221 tccttctaag tagaaaatca tagacaggat aatccttaaa aatcacaaat gatttaacta
    17281 atgcattgag aatgatgacg gctcggtcaa caagtcatca acagacaata accacgtaat
    17341 catgcttctc tgacatttta tcggagaagc aatgactctt tgtctcagta ccctcctcct
    17401 ttttaaaagg cagatttcag tcgctaatac aacacatttt taaatacact ttaacctggt
    17461 tcattattac acatctaaaa c
//
LOCUS       DAPPNJ010000093        17452 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000093 DAPPNJ010000000
VERSION     DAPPNJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.093"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            74..1213
                     /gene="arnB"
                     /locus_tag="RPM73_002551"
     CDS             74..1213
                     /gene="arnB"
                     /locus_tag="RPM73_002551"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDX6520898.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1217..2185
                     /gene="arnC"
                     /locus_tag="RPM73_002552"
     CDS             1217..2185
                     /gene="arnC"
                     /locus_tag="RPM73_002552"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDX6520899.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2185..4167
                     /gene="arnA"
                     /locus_tag="RPM73_002553"
     CDS             2185..4167
                     /gene="arnA"
                     /locus_tag="RPM73_002553"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDX6520900.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAHLAPEVIFSFYYRHLICDAILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPDNEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLNFFLRTVDLTDKPS"
     gene            4164..5054
                     /locus_tag="RPM73_002554"
     CDS             4164..5054
                     /locus_tag="RPM73_002554"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDX6520901.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSSNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5054..6706
                     /gene="arnT"
                     /locus_tag="RPM73_002555"
     CDS             5054..6706
                     /gene="arnT"
                     /locus_tag="RPM73_002555"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDX6520902.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFVALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6703..7038
                     /gene="arnE"
                     /locus_tag="RPM73_002556"
     CDS             6703..7038
                     /gene="arnE"
                     /locus_tag="RPM73_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDX6520903.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7038..7424
                     /gene="arnF"
                     /locus_tag="RPM73_002557"
     CDS             7038..7424
                     /gene="arnF"
                     /locus_tag="RPM73_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDX6520904.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="RPM73_002558"
     CDS             complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="RPM73_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDX6520905.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7794..9149)
                     /gene="menE"
                     /locus_tag="RPM73_002559"
     CDS             complement(7794..9149)
                     /gene="menE"
                     /locus_tag="RPM73_002559"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDX6520906.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9146..10108)
                     /gene="menC"
                     /locus_tag="RPM73_002560"
     CDS             complement(9146..10108)
                     /gene="menC"
                     /locus_tag="RPM73_002560"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDX6520907.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10108..10965)
                     /gene="menB"
                     /locus_tag="RPM73_002561"
     CDS             complement(10108..10965)
                     /gene="menB"
                     /locus_tag="RPM73_002561"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDX6520908.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10980..11738)
                     /gene="menH"
                     /locus_tag="RPM73_002562"
     CDS             complement(10980..11738)
                     /gene="menH"
                     /locus_tag="RPM73_002562"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDX6520909.1"
                     /translation="MNLHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11735..13405)
                     /gene="menD"
                     /locus_tag="RPM73_002563"
     CDS             complement(11735..13405)
                     /gene="menD"
                     /locus_tag="RPM73_002563"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDX6520910.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPACWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13494..14789)
                     /gene="menF"
                     /locus_tag="RPM73_002564"
     CDS             complement(13494..14789)
                     /gene="menF"
                     /locus_tag="RPM73_002564"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDX6520911.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="RPM73_002565"
     CDS             complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="RPM73_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDX6520912.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15228..15689)
                     /locus_tag="RPM73_002566"
     CDS             complement(15228..15689)
                     /locus_tag="RPM73_002566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX6520913.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15754..16671
                     /gene="rbn"
                     /locus_tag="RPM73_002567"
     CDS             15754..16671
                     /gene="rbn"
                     /locus_tag="RPM73_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDX6520914.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16859..>17452
                     /locus_tag="RPM73_002568"
     CDS             16859..>17452
                     /locus_tag="RPM73_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDX6520915.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSEDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYV"
BASE COUNT         4102 a         4697 c         4413 g         4240 t
ORIGIN      
        1 aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc
       61 ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga
      121 actcgctgca gttaaagagg ttctcgaatc cggttggatc acaaccggtc cgaagaatca
      181 ggcgcttgag caagcctttt gccagttgac gggaaatcag catgccatcg cggtcagttc
      241 agccaccgcc ggaatgcata tcacgctaat ggcgttgaaa attggcaagg gcgatgaagt
      301 gattacgcct tccctgacct gggtttcaac cctcaatatg atttccttgt tgggtgcaac
      361 gccggtaatg gtggatgtcg accgcgatac gctgatggtc acgcctgaag ctatcgagtc
      421 agccattacg ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga
      481 tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc
      541 ccatgccgtc ggtacgtatt acaaagggcg acatattggc gcaaaaggta ccgctatttt
      601 ttcatttcat gccattaaaa atattacctg tgctgaaggt ggcctgattg taactgataa
      661 tgaaaacctt gcccgccagc tacggatgct gaaatttcac ggtctgggtg tcgatgccta
      721 tgacagacaa acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg gctataagta
      781 caatctgacc gatattaacg ccgcgattgc cctgacacag ttagtcaaat tagagcacct
      841 caacacccgt cggcgcgaaa ttgcccagca atatcagcaa gcactggcag cactcccctt
      901 tcagccatta agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg
      961 tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaaag
     1021 aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg
     1081 ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct gttctttgcc
     1141 gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact
     1201 cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc
     1261 cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacca cagcctgtga
     1321 atcgttgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc
     1381 gcatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa
     1441 ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta ctggcgactt
     1501 aattattacc cttgatgccg atctccagaa tccgccagaa gaaatccccc gcctggtggc
     1561 aaaagccgat gaaggttacg acgtggtagg gactgtacgc cagaaccgcc aggacagctg
     1621 gtttcgtaaa acagcttcga agatgattaa ccggcttatt cagcgcacca ctggcaaagc
     1681 gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt
     1741 gcactgccat gaacgcagca cctttatccc gattctggcg aatatcttcg cccgccgtgc
     1801 aattgaaatt ccagtacatc atgccgagcg tgagtttggt gaatccaaat acagttttat
     1861 gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat
     1921 gctgagtctg ctcggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt
     1981 gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc
     2041 cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattactcg gtgaatatat
     2101 cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg
     2161 tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga
     2221 tgcctcggta ttgaagccct gctggctgcc ggttacgaaa ttagcgctat ttttactcat
     2281 actgataatc ccggtgaaaa agccttttat ggttcggtag ctcgtctggc ggcggaaaga
     2341 ggcattccgg tttatgcgcc ggataacgtt aatcatccgc tgtgggtgga acgcattgcc
     2401 cacctagcac cagaggtgat tttctctttt tattatcgcc atcttatttg cgacgcgatt
     2461 ttgcagctcg ctcccgcggg tgcatttaat ctgcacggtt cgctgttacc aaaatatcgt
     2521 ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa cggaaactgg cgttacattg
     2581 caccgaatgg tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg cattgccatt
     2641 gcgccagacg atatcgccat tacgctgcat cataaattgt gccatgccgc gcgccagttg
     2701 ctggaacaga cattacccac cattaaacac ggtaatattc tggaaattgc ccagcgcgaa
     2761 aacgaagcca cctgttttgg tcgcagaacg ccagatgaca gtttcctcga atggcataaa
     2821 ccggcatccg tgctgcacaa catggtgcgt gccgttgccg atccgtggcc aggtgccttc
     2881 agctatgttg gtaatcagaa atttaccgtc tggtcgtcgc gtgttcatcc tcatgccagc
     2941 aaagcacagc cggggagcgt gatttctatt gcaccgctgt tgattgcctg tggcgatggc
     3001 gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg ctcgcaatta
     3061 gcgcagacgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgcaccgcc
     3121 cgacgccgta cccgggtact catcctcggg gtaaatggct ttattggcaa ccatctgaca
     3181 gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg cagcgatgcg
     3241 ataagccgtt ttctgaatca tccgcatttt cactttgtcg aaggcgatat cagtattcat
     3301 tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata
     3361 gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag
     3421 aatctgcgca ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt cccgtcaact
     3481 tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc taatttaatc
     3541 gtcggcccgg tgaataaacc acgctggatt tattcggtgt caaaacaatt acttgatcgg
     3601 gttatctggg cctatggcga aaaagagggt ttacagttca ccctcttccg cccgtttaac
     3661 tggatgggac cacgactgga taaccttaat gcagcgcgaa ttggcagctc ccgcgcgatt
     3721 acgcaactca ttctcaatct ggtagaaggt tcaccgatta agctgattga tggcggaaaa
     3781 caaaaacgct gctttactga tatccgggat ggtatcgagg cgttgtaccg cattatcgaa
     3841 aatgcgggaa atcgctgcga cggcgaaatt atcaacattg gcaatcctga taacgaagcg
     3901 agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgtcat
     3961 catttcccac cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga
     4021 tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccaccgctg cctggactgg
     4081 gagccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tgaatttctt cctgcgcacc
     4141 gttgatctta cggataaacc atcatgacca aagtaggctt acgcattgat gtcgatacct
     4201 ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag cataatattc
     4261 aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg
     4321 tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt tatggctggg
     4381 atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc aatgccgata
     4441 tcattcgtga agcggctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct
     4501 ggcaagcccg tagcagtaac tgggatcggc aaacaatgat cgacgatatt gcacgcggtc
     4561 ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct gcgggctggc
     4621 gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt
     4681 gtcgtggggc catgccgttc cgtccattgc tcgaatcagg aaaccctggc actgcgcaaa
     4741 ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt
     4801 ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt tataccattc
     4861 atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc aaacgcgcag
     4921 ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg ttgccgctcg
     4981 gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc caacaaattg
     5041 cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg
     5101 ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa cgcgttatgc
     5161 ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccatc tgttagggct
     5221 acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt
     5281 tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc
     5341 gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccac
     5401 agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg tgctcgatcc
     5461 gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca
     5521 gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg gcatgggggt
     5581 gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc catgggtagc
     5641 gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta tcagttgcgt
     5701 actgacggtt ctcccttggg gactggcgat agcgcagcgg gagcctgact tctggcacta
     5761 ttttttctgg gttgagcata ttcaacgctt tgcactggat gatgcccaac atagagctcc
     5821 gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg gattactccc
     5881 cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag
     5941 ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc ccacctatat
     6001 tctttcctgc tttgcatctc tggcaatgct gatggcgcat tacgctttgc tggcagcaaa
     6061 aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg gcgtcactgg
     6121 cattattgcc acatttgtgg tctccccgtg gggaccaatg aacacgccgg tgtggcaaac
     6181 cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg
     6241 ctggtacacc ttaacaaacg tcgaaaagac ctggcctttt gtcgcgcttt gcccgctggg
     6301 gctggcgttg ctggtaggat tttcaattcc tgacagagtt atggaaggaa aacatccgca
     6361 attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag
     6421 cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg
     6481 ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag
     6541 cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttactctcgt
     6601 gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg cagatgccat
     6661 cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta
     6721 gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt
     6781 gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt
     6841 cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc
     6901 tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac
     6961 gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg cattgtgatc
     7021 ctcgggagta cggtgtaatg ggcctgatat ggggattatt cagcgtcatt attgcctcag
     7081 ttgcgcaatt aagcctgggt tttgcggcaa gtcatctgcc gccgatgacg cacttatggg
     7141 attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta ctcgggctgc
     7201 tgggttatct gctttccgtt ttttgttggt ataaaacgct acataaactt gcgctaagca
     7261 aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc
     7321 ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg
     7381 ggttgatgct gattttcctg cccacgacaa aacaacgtta ctgagttttc cctgccgctg
     7441 tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat
     7501 gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca
     7561 ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc
     7621 agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg accagccatt
     7681 ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac
     7741 tctttatcaa cggcaatgga atggcagaaa tgagaagtga aacggagggt taattattgc
     7801 tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagc
     7861 tccggcggta aagtcagcca gcgcaccggt tgttgaaaac gagccagctt atctttcacc
     7921 cattcgccaa gatcaacggt ttgctggtca tactccacca ccgctaccgg ccgatgacca
     7981 aactccttgt cggcaacggg aacgataaac acctgcaaaa ccgcaggatg tgcagcaata
     8041 acacgctcga cttcttccgg ctgaataccc tcaccgccac tgaagaacag attgtctaaa
     8101 cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag
     8161 ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg
     8221 gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa
     8281 ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga
     8341 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg
     8401 atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa
     8461 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
     8521 ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc
     8581 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga
     8641 atcaacgata acaccccttc ggcactggca agatgggcct gataagtatg gacagcggct
     8701 ttcggcaacc cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac
     8761 gtagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaacgc cggaaacgtg
     8821 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
     8881 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccacattg cagtaacgcc
     8941 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
     9001 acaacgccct gcgccgcaaa gcctgaggct aattcatcga cgcgagcaca aagctcgcgc
     9061 cagttaagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag
     9121 tgacgccacg gccagtcaga gaagatcata acaaccgctc cagtgcatca acttccacga
     9181 caggcagcgt gctacccggc cagcgacgta cctgctgcgc ctgcatcaga tccagcgtgt
     9241 ccagccctgg aatggtgtcc ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc
     9301 ctaagctcga ttcaatggaa gaactgatca ccgccgtcag ccccagcgcg tgcgccgcct
     9361 gtacctgctc gcgtactttc tccagactgc ccgtgagcgt gggtttgata actaccgcgc
     9421 gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caggctttca tcccaggcaa
     9481 tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt
     9541 cgagaaacgc gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctggcctt
     9601 tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
     9661 gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta caatcccact ttgaccttcg
     9721 ccactttctc gcctggcata tcagcaagtt tgaggatcag atcgtcagga tcgccattac
     9781 acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt atctgccagc tccgccaatg
     9841 cacagcttac gccaaaggca acggaaggca tctgcggtag ctcgcaatcg cctgccagcc
     9901 agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc
     9961 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca
    10021 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct
    10081 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg
    10141 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag
    10201 catggtggcg ttgcccgcca gctcttgcag ccccgcctgc ccgtcacagt cggcgttcag
    10261 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    10321 gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag
    10381 cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tctcgcgctt ttttctgccc
    10441 gacgatacga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    10501 agtctgaccg aagatggcat tatctgctgc gatagtcagg tcgcacatca tgtgcagaac
    10561 gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    10621 gatctgacgc tggaagtcca gcacgttcaa gtgatgtacg ccggaatcat ctttatagcc
    10681 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    10741 agtcagaatg atcacgccga tattgtcgtc ataacgcgca tctgccagtg cctggatcat
    10801 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    10861 tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg
    10921 ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt
    10981 cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc ccgatgcgcg
    11041 ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc cagggcgcgg
    11101 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    11161 gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg
    11221 ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca
    11281 aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt gcaaaaacgc
    11341 tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc
    11401 ggatgcccgc cttcgacaat aaccccacaa agccccgcca gcccctggca agccgccatc
    11461 atcgccaccc gtccgccaag cgagtacccc accagccaga agtcaaggat gttgtaacta
    11521 accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc
    11581 gaaccaccat gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc
    11641 acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    11701 cctagttttc cgtgttttgc ctgcgcgtgc aggttcataa atggcttacc tgcgccagaa
    11761 gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc accgtggtgg
    11821 ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt tgcggacgat
    11881 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    11941 agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc ccgccgttgt
    12001 tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc agcgcgttga
    12061 gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct
    12121 gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg ttgctgtaca
    12181 ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    12241 caacaaacaa ttgcccctgt tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt
    12301 cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga
    12361 tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat
    12421 tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa
    12481 caatccagta ctcttcgggt tcacagcttg cctgccattg caggagccgt ttccccgtca
    12541 ggctgcttcc cagttgcacc acaatttgcg cctgctgcag ctcgctggtc gctttggcat
    12601 tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc acatcgccaa
    12661 tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct tccgcactca
    12721 tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt
    12781 cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcaccca
    12841 gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg
    12901 ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa
    12961 ccagccaaca tgcggggata tcctgggtcg ggcgcggcaa tgaaatactg tgcgtggggt
    13021 gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg
    13081 gcgggcgatc ggcggttaag agaatcagtt tttctccggt taacccggct tcaatcagtg
    13141 ccggatagag atttgccacc gccgtgccgg aggtcacaat caccgccacc ggctgcttgc
    13201 tgactttcgc cagccccagc gccagatgcc ccagtccacg ctcatcgaaa tgggtgtggt
    13261 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagcctgggg
    13321 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    13381 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    13441 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    13501 atctgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    13561 gggtcggaac cacggacaat gcccgcgcca gcatataatc gcacgacatt gccgctaatt
    13621 tttgctgagc gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa
    13681 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    13741 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc
    13801 gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    13861 acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct
    13921 tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca ctctcctaac
    13981 tgctgcgcct gcttatcatc aggattattt gctactgttc ccgccagcgc ttcagtacgc
    14041 agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg
    14101 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc
    14161 atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact
    14221 ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    14281 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    14341 atactcacca gtgtggcgat aaattctttt gcctgaatcg catcgtgctg aagggagctt
    14401 tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgg
    14461 ggtaaaagta aattgccctg cgacgggtca aaagcattca gcccccaaat gcgtaagtcg
    14521 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    14581 atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg
    14641 tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    14701 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    14761 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    14821 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    14881 agcaacagtc ctagtaccag cccaacggcc gcgcccacac caattccttg ccagggtttt
    14941 tcgtggacgt agtcatcagc acgataaact gcctgcttcg cccgatagta ataactgtct
    15001 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    15061 tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca
    15121 ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    15181 ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta cgcctgaatt
    15241 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    15301 ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc
    15361 ttatcaggcc agtgatgcgt acatgtttcg agtgttttac tcatcagctg ctgccccact
    15421 ttttcgccac gcaacgcttc actgacaatc acccgaccta taacgaccgg ctcaagatca
    15481 tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata
    15541 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg
    15601 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    15661 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    15721 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    15781 gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    15841 acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    15901 accgccttta accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat
    15961 ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    16021 acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg gattagcggc
    16081 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    16141 ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta tggctatcgt
    16201 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    16261 ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga
    16321 aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt
    16381 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    16441 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    16501 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggtaagct aatcattacc
    16561 cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgcaggtca
    16621 attttcccgg cgactgaact agcgaatgat ttcaccgtgt ttaacgttta actctgctta
    16681 ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc
    16741 acacgggcat attgagcgat ttttgatatt tattcatctc ctcacgcact atattcagag
    16801 caataagttg cctctattta tcttgattca gcacaacctt tgcaactaaa aggagcccat
    16861 gatggttaca gttgtcagca attattgtca attatctcaa acgcaactca gtcagacatt
    16921 tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc
    16981 cggagatgaa gaaagcattg agttactgca taatattgcg ttaggctatg atgaatttgg
    17041 aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    17101 gattatccga cttatcaaaa atgcttgttt aaaattatat aatttggcac atgccgcaac
    17161 caactccccc cttaaaccag ctggtccgga taattcagag gatctcctgt tcaaaaagct
    17221 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
    17281 acagtcgctt tcgaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
    17341 cttctgggat aaaaatcgtc aattaaccac agatgaaata gattgctatc ttcagaagat
    17401 cgccgctaat gcaaaaaata ctgaagtcaa ttatcctact ggtctctacg tc
//
LOCUS       DAPPNJ010000094        17378 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000094 DAPPNJ010000000
VERSION     DAPPNJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.094"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(27..1310)
                     /gene="gltA"
                     /locus_tag="RPM73_002569"
     CDS             complement(27..1310)
                     /gene="gltA"
                     /locus_tag="RPM73_002569"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDX6520916.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1516..1623)
                     /locus_tag="RPM73_002570"
     CDS             complement(1516..1623)
                     /locus_tag="RPM73_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520917.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2004..2408
                     /gene="sdhC"
                     /locus_tag="RPM73_002571"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2004..2408
                     /gene="sdhC"
                     /locus_tag="RPM73_002571"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDX6520918.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2402..2749
                     /gene="sdhD"
                     /locus_tag="RPM73_002572"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2402..2749
                     /gene="sdhD"
                     /locus_tag="RPM73_002572"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDX6520919.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2749..4515
                     /gene="sdhA"
                     /locus_tag="RPM73_002573"
     CDS             2749..4515
                     /gene="sdhA"
                     /locus_tag="RPM73_002573"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDX6520920.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4531..5247
                     /gene="sdhB"
                     /locus_tag="RPM73_002574"
     CDS             4531..5247
                     /gene="sdhB"
                     /locus_tag="RPM73_002574"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDX6520921.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5448..8249
                     /gene="sucA"
                     /locus_tag="RPM73_002575"
     CDS             5448..8249
                     /gene="sucA"
                     /locus_tag="RPM73_002575"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDX6520922.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQLHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8264..9481
                     /gene="odhB"
                     /locus_tag="RPM73_002576"
     CDS             8264..9481
                     /gene="odhB"
                     /locus_tag="RPM73_002576"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDX6520923.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9575..10741
                     /gene="sucC"
                     /locus_tag="RPM73_002577"
     CDS             9575..10741
                     /gene="sucC"
                     /locus_tag="RPM73_002577"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDX6520924.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10741..11610
                     /gene="sucD"
                     /locus_tag="RPM73_002578"
     CDS             10741..11610
                     /gene="sucD"
                     /locus_tag="RPM73_002578"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDX6520925.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11714..12436)
                     /gene="mngR"
                     /locus_tag="RPM73_002579"
     CDS             complement(11714..12436)
                     /gene="mngR"
                     /locus_tag="RPM73_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDX6520926.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12605..14521
                     /gene="mngA"
                     /locus_tag="RPM73_002580"
     CDS             12605..14521
                     /gene="mngA"
                     /locus_tag="RPM73_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDX6520927.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14539..17172
                     /gene="mngB"
                     /locus_tag="RPM73_002581"
     CDS             14539..17172
                     /gene="mngB"
                     /locus_tag="RPM73_002581"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDX6520928.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLRDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4191 a         4343 c         4737 g         4107 t
ORIGIN      
        1 aatatttaca acttagcaat caaccattaa cgcttgatat cgcttttaaa gtcgcgtttt
       61 tcatatcctg tatacagctg acgcggacgg gcaatcttca taccgtcact gtgcatttcg
      121 ctccagtggg cgatccagcc aacggtacgt gccattgcga aaatgacggt gaacatggaa
      181 gacggaatac ccatcgcttt caggatgata ccagagtaga aatcgacgtt cgggtacagt
      241 ttcttctcga taaagtacgg gtcgttcagc gcgatgtttt ccagctccat agccacttcc
      301 agcaggtcat ctttggtgcc cagctctttc agaacttcat ggcaggtttc acgcattacg
      361 gtggcgcgcg ggtcgtaatt tttgtacacg cggtgaccga agcccatcag gcggaaagaa
      421 tcatttttat ctttcgcacg acgaacaaat tccggaatgt gtttaacgga gctaatttct
      481 tccagcattt tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca cagtgaagca
      541 atacctgctg cgatacaggc aaacgggttc gcacccgaag agccagcggt acgcacggtg
      601 gaggtagagg cgttctgttc atggtcagcg tgcaggatca gaatacggtc catagcacgt
      661 tccagaatcg gattaacttc atacggttcg cacggcgtgg agaacatcat attcaggaag
      721 ttaccggcat aggagagatc gttgcgcgga taaacaaatg gctgaccaat ggaatacttg
      781 taacacatcg cggccatggt cggcattttc gacagcaggc ggaacgcggc aatttcacga
      841 tgacgaggat tgttaacatc cagcgagtcg tgatagaacg ccgccagcgc gccggtaata
      901 ccacacatga ctgccattgg atgtgagtcg cgacggaaag cgtggaacag acgggtaatc
      961 tgctcgtgga tcatggtatg acgggtcacc gtagttttaa attcgtcata ctgttcctga
     1021 gtcggttttt caccattcag caggatgtaa caaacttcca ggtagttaga atcggtcgcc
     1081 agctgatcga tcgggaaacc gcggtgcagc aaaatacctt catcaccatc aataaaagta
     1141 attttagatt cgcaggatgc ggttgaagtg aagcctgggt caaaggtgaa cacacctttt
     1201 gaaccgagag tacggatatc aataacatct tgacccagcg tgcctttcag cacatccagt
     1261 tcaacagctg tgtccccgtt gagggtgagt tttgcttttg tatcagccat ttaaggtctc
     1321 cttagcgcct tattacgtaa gactgccgga acttaaattt gccttcgcac atcaacctgg
     1381 ctttacccgt ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc tgggtacaga
     1441 gctctgggcg cttgcaggta aaggatccat tgatgacgaa taaatggcga atcaagtact
     1501 tagcaatccg aattattaaa cttgtctacc actaataact gtcccgaatg aattggtcaa
     1561 tacttccaca ctgttacata agttaatctt aggtgaaata ccgacttcat aacttttacg
     1621 cattatatgc ttttcctggt aatgtttgta acaactttgt tgaatgattg tcaaattaga
     1681 tgattaaaaa ttaaataaat gttgttatcg tgacctggat cactgttcag gataaaaccc
     1741 gacaaactat atgtaggtta attgtaatga ttttgtgaac agcctatact gccgccaggt
     1801 ctccggaaca ccctgcaatc ccgagccacc cagcgttgta acgtgtcgtt ttcgcatctg
     1861 gaagcagtgt tttgcatgac gcgcagttat agaaaggacg ctgtctgacc cgcaagcaga
     1921 ccggaggaag gaaatcccga cgtctccagg taacagaaag ttaacctctg tgcccgtagt
     1981 ccccagggaa taataagaac agcatgtggg cgttattcat gataagaaat gtgaaaaaac
     2041 aaagacctgt taatctggac ctacagacca tccggttccc catcacggcg atagcgtcca
     2101 ttctccatcg cgtttccggt gtgatcacct ttgttgcagt gggcatcctg ctgtggcttc
     2161 tgggtaccag cctctcttcc cctgaaggtt tcgagcaagc ttccgcgatt atgggcagct
     2221 tcttcgtcaa atttatcatg tggggcatcc ttaccgctct ggcgtatcac gtcgtcgtag
     2281 gtattcgcca catgatgatg gattttggct atctggaaga aacattcgaa gcgggtaaac
     2341 gctccgccaa aatctccttt gttattactg tcgtgctttc acttctcgca ggagtcctcg
     2401 tatggtaagc aacgcctccg cattaggacg caatggcgta catgatttca tcctcgttcg
     2461 cgctaccgct atcgtcctga cgctctacat catttatatg gtcggttttt tcgccaccag
     2521 tggcgaactg acatatgaag tctggatcgg tttcttcgcc tctgcgttca ccaaagtgtt
     2581 caccctgctg gcgctgtttt ctatcttgat tcatgcctgg atcggcatgt ggcaggtgtt
     2641 gaccgactac gttaaaccgc tggcattgcg cctgatgctg caactggtga ttgtcgttgc
     2701 actggtggtt tacgtgattt atggattcgt tgtggtgtgg ggtgtgtgat gaaattgcca
     2761 gtcagagaat ttgatgcagt tgtgattggt gccggtggcg caggtatgcg cgcggcgctg
     2821 caaatttccc agagcggcca gacctgtgcg ctgctctcta aagtcttccc gacccgttcc
     2881 cataccgttt ctgcgcaagg cggcattacc gttgcgctgg gtaataccca tgaagataac
     2941 tgggaatggc atatgtacga caccgtaaaa gggtcggact atatcggtga ccaggacgcg
     3001 attgaatata tgtgtaaaac cgggccggaa gcgattctgg aactcgaaca catgggcctg
     3061 ccgttctcgc gtcttgatga tggtcgtatc tatcaacgtc cgtttggcgg tcagtcgaaa
     3121 aacttcggcg gcgagcaggc ggcacgcact gcggcggcgg ctgaccgtac cggtcacgca
     3181 ctgttgcaca cgctttatca gcagaacctg aaaaaccaca ccaccatttt ctccgagtgg
     3241 tatgcgctgg atctggtgaa aaaccaggat ggcgcggtgg tgggttgtac cgcactgtgc
     3301 atcgaaaccg gtgaagtggt ttatttcaaa gcccgcgcta ccgtgctggc gactggcggg
     3361 gcagggcgta tttatcagtc caccactaac gcccacatta acactggcga cggcgtcggc
     3421 atggctatcc gcgcaggcgt accggtgcag gatatggaaa tgtggcagtt ccacccgacc
     3481 ggtattgccg gtgcgggcgt actggtcacc gaaggttgcc gtggtgaagg cggttatctg
     3541 ctgaacaaac atggcgaacg ctttatggag cgttatgcgc cgaacgccaa agacctggcg
     3601 ggccgtgacg tggtggcgcg ttccatcatg attgaaatcc gtgaaggtcg cggctgtgat
     3661 ggtccgtggg ggccacatgc gaaactgaaa ctcgaccacc tgggtaaaga agttctcgaa
     3721 tcccgtctgc cgggtattct ggaactctcc cgtaccttcg ctcacgttga tccggtgaaa
     3781 gagccgattc cggttatccc aacctgtcac tacatgatgg gtggtattcc gaccaaagtg
     3841 accggtcagg cgctgacggt gaacgagaaa ggcgaagatg tggttgttcc ggggctgttt
     3901 gccgttggtg aaatcgcttg tgtatcggta cacggcgcta accgtctggg cggcaactcg
     3961 ctgctggacc tggtggtctt tggtcgcgca gcgggtctgc atctgcaaga gtctatcgcc
     4021 gagcagggcg cattgcgcga tgccagcgag tctgatgttg aagcatctct ggatcgtctg
     4081 aaccgctgga acaataatcg taacggtgaa gatccggtgg cgattcgtaa agcgctgcaa
     4141 gaatgtatgc agcataactt ctcggtcttc cgtgaaggtg atgcgatggc gaaagggctt
     4201 gagcagttga aagttatccg cgagcgtctg aaaaatgccc gtctggatga cacttccagc
     4261 gagttcaaca cccagcgcgt tgagtgcctg gaactggata acctgatgga aacggcgtat
     4321 gcaacggctg tttctgccaa cttccgtacc gaaagccgtg gcgcgcatag ccgcttcgac
     4381 ttcccggatc gtgatgatga aaactggctg tgccactccc tgtatctgcc agagtcggaa
     4441 tccatgacgc gccgaagcgt caacatggaa ccgaaactgc gcccggcatt cccgccgaag
     4501 attcgtactt actaatgcgg agacaggaaa atgagactcg agttttcaat ttatcgctat
     4561 aacccggatg ttgatgatgc tccgcgtatg caggattaca ccctggaagc ggaagaaggt
     4621 cgcgacatga tgttgctgga tgcgcttatc cagctgaaag agaaagatcc cagcctgtcg
     4681 ttccgccgct cctgccgtga aggtgtgtgc ggttccgacg gtctgaacat gaacggcaag
     4741 aatggtctgg cctgtattac cccgatttcg gcactcaacc agccgggcaa gaagattgtg
     4801 attcgcccgc tgccaggttt accggtgatc cgcgatttgg tggtagacat gggacaattc
     4861 tatgcgcaat atgagaaaat taagccttac ctgttgaata atggacaaaa tccgccagct
     4921 cgcgagcatt tacagatgcc agagcagcgc gaaaaactcg acgggctgta tgaatgtatt
     4981 ctctgcgcat gttgttcaac ctcttgtccg tctttctggt ggaatcccga taagtttatc
     5041 ggcccggcag gcttgttagc ggcatatcgt ttcctgatcg atagccgtga taccgagact
     5101 gacagccgcc tcgacggttt gagcgatgca ttcagtgtat tccgctgtca cagcatcatg
     5161 aactgcgtca gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg ccatatcaag
     5221 tcgatgttgt tgcaacgtaa tgcgtaatta aatgttgatg ccggggaaat cccccggcta
     5281 ttcaggaaat ctttaaaaac tgcccctgac actaagacag tttttaaagg ttccttcgcg
     5341 agccactacg tagacaagag ctcgcaagtg aaccccggca cgcacataac tgtgcgtggt
     5401 agtatccacg gcgaagtaag cataaaaaag atgcttaagg gatcacgatg cagaacagcg
     5461 ctttgaaagc ctggttggac tcttcttacc tctctggcgc aaaccagagc tggatagaac
     5521 agctctatga agacttctta accgatcctg actcggttga cgctaactgg cgttcgacgt
     5581 tccagcagtt acctggtacg ggagtcaaac cggatcaact ccactctcaa acgcgtgaat
     5641 atttccgccg cctggcgaaa gacgcttcac gttactcttc aacgatctcc gaccctgaca
     5701 ccaatgtaaa gcaggttaaa gtcctgcagc tcattaacgc ataccgcttc cgtggtcacc
     5761 agcatgcgaa tctcgatccg ctgggactgt ggcagcaaga taaagtggcc gatctggatc
     5821 cgtctttcca cgatctgacc gaagcagact tccaggagac cttcaacgtc ggttcatttg
     5881 ccagcggcaa agaaaccatg aaactcggcg agctgctgga agccctcaag caaacctact
     5941 gcggcccgat tggtgccgag tatatgcaca ttaccagcac cgaagaaaaa cgctggatcc
     6001 aacagcgtat cgagtctggt cgcgcgactt tcaatagcga agagaaaaaa cgcttcttaa
     6061 gcgaactgac cgccgctgaa ggccttgaac gttacctcgg cgcaaaattc cctggcgcaa
     6121 aacgcttctc gctggaaggc ggtgacgcgt taatcccgat gcttaaagag atgatccgcc
     6181 acgctggcaa cagcggcacc cgggaagtgg ttctcgggat ggcgcaccgt ggtcgtctga
     6241 acgtgttggt gaacgtgctg ggtaaaaaac cgcaagactt gttcgacgag tttgccggta
     6301 aacataaaga acacctcggc acgggtgacg tgaaatacca catgggcttc tcgtctgact
     6361 tccagaccga tggcggcctg gtgcacctgg cgctggcgtt taacccgtct caccttgaga
     6421 ttgtaagccc ggtcgttatc ggctctgttc gtgcccgtct ggacagactt gatgagccga
     6481 gcagcaacaa agtgctgcca attaccatcc acggtgacgc cgcagtgacc ggacagggcg
     6541 tggttcagga aaccctgaac atgtcgaaag cgcgtggtta tgaagttggc ggtacggtac
     6601 gtatcgttat caacaaccag gttggtttca ccacctctaa tccgctggat gcccgttcta
     6661 cgccgtactg tactgatatc ggcaagatgg ttcaggcacc gattttccac gttaacgcgg
     6721 acgatccgga agccgttgcc tttgtgaccc gtctggcgct cgatttccgt aacaccttta
     6781 aacgtgatgt cttcatcgac ctggtatgct accgccgtca cggccacaac gaagccgacg
     6841 agccgagcgc aacccagccg ctgatgtatc agaaaatcaa aaaacatccg acaccgcgca
     6901 aaatctacgc tgacaagctg gagcaggaaa aagtggcgac gctggaagat gccaccgaga
     6961 tggttaacct gtaccgcgat gcgctggatg ctggcgattg cgtggtagca gagtggcgtc
     7021 cgatgaacat gcactctttc acctggtcgc cgtacctcaa ccacgaatgg gacgaagagt
     7081 acccgaacaa agttgagatg aagcgcctgc aggaactggc taaacgcatc agcacggtgc
     7141 cggaagcggt tgaaatgcag tctcgcgttg ccaagattta tggcgatcgc caggcgatgg
     7201 ctgccggtga gaaactgttc gactggggcg gcgcggaaaa cctcgcttac gccacgctgg
     7261 ttgacgaagg cattccggtt cgcctgtcgg gtgaagactc cggtcgcggt accttcttcc
     7321 accgccacgc ggtgatccac aaccagtcta acggttccac ttacacgccg ctgcaacaca
     7381 tccataacgg ccagggcgcg ttccgtgttt gggactccgt actgtctgaa gaagcagtgc
     7441 tggcgtttga atacggttat gccaccgcag aaccacgcac cctgaccatc tgggaagcgc
     7501 agttcggtga cttcgccaac ggtgcgcagg tggttatcga ccagttcatc tcctctggcg
     7561 agcagaaatg gggccggatg tgtggtctgg tgatgttgct gccgcacggt tacgaagggc
     7621 aggggccgga gcactcctcc gcgcgtctgg aacgttatct gcaactttgt gctgagcaaa
     7681 acatgcaggt ttgcgtaccg tctaccccgg cacaggttta ccacatgctg cgtcgtcagg
     7741 cgctgcgcgg gatgcgtcgt ccgctggtcg tgatgtcgcc gaaatccctg ctgcgtcatc
     7801 cgctggcggt ttccagcctc gaagaactgg cgaacggcac cttcctgcca gccatcggtg
     7861 aaatcgacga gcttgatccg aagggcgtga agcgcgtagt aatgtgttct ggtaaggttt
     7921 attacgacct gctggaacag cgtcgtaaga acaatcaaca cgatgtcgcc attgtgcgta
     7981 tcgagcaact ctacccgttc ccgcataaag cgatgcagga agtgttgcag cagtttgctc
     8041 acgtcaagga ttttgtctgg tgccaggaag agccgctcaa ccagggcgca tggtactgca
     8101 gccagcatca tttccgtgaa gtgattccgt ttggggcttc tctgcgttat gcaggccgcc
     8161 cggcctccgc ctctccggcg gtagggtata tgtccgttca ccagaaacag caacaagatc
     8221 tggttaatga cgcgctgaac gtcgaataaa taaaggatac acaatgagta gcgtagatat
     8281 tctggtccct gacctgcctg aatccgtagc cgatgccacc gtcgcaacct ggcataaaaa
     8341 acccggcgac gcagtcgtac gtgatgaagt gctggtagaa atcgaaactg acaaagtggt
     8401 actggaagta ccggcatcag cagacggcat tctggatgcg gttctggaag atgaaggtac
     8461 aacggtaacg tctcgtcaga tccttggtcg cctgcgtgaa ggcaacagcg ccggtaaaga
     8521 aaccagcgcc aaatctgaag agaaagcgtc cactccggcg caacgccagc aggcgtctct
     8581 ggaagagcaa aacaacgatg cgttaagccc ggcgatccgt cgcctgctgg ctgaacacaa
     8641 tctcgacgcc agcgccatta aaggcaccgg tgtgggtggt cgtctgactc gtgaagatgt
     8701 ggaaaaacat ctggcgaaag ccccggcgaa agagtctgct ccggcagcgg ctgatccggc
     8761 ggcgcaaccg gctctggctg cacgtagtga aaaacgtgtc ccgatgactc gcctgcgtaa
     8821 gcgtgtggca gagcgtctgc tggaagcgaa aaactccacc gccatgctga ccacgttcaa
     8881 cgaagtcaac atgaagccga ttatggatct gcgtaagcag tacggtgaag cgtttgaaaa
     8941 acgccacggc atccgtctgg gctttatgtc cttctacgtg aaagcggtgg ttgaagccct
     9001 gaaacgttac ccggaagtga acgcgtctat cgacggcgat gacgtggttt accacaacta
     9061 tttcgacgtc agcatggcgg tttctacgcc gcgcggcctg gtgacaccgg tactgcgtga
     9121 tgtcgatacc ctcggcatgg cagacatcga gaagaaaatc aaagagctgg cagtcaaagg
     9181 ccgtgacggc aagctgaccg ttgaagatct gaccggtggt aacttcacca tcaccaacgg
     9241 tggtgtgttc ggttccctga tgtctacgcc gatcatcaac ccgccgcaga gcgcaattct
     9301 gggtatgcac gctatcaaag atcgtccgat ggcggtgaat ggtcaggttg agatcctgcc
     9361 gatgatgtac ctggcgctgt cctacgatca ccgtctgatc gatggtcgcg aatccgtggg
     9421 cttcctggta acgatcaaag agttgctgga agatccgacg cgtctgctgc tggacgtgta
     9481 gtagtttaag cttcacctgc actgtagacc gacgtggata gggtgtcggt ctacggttta
     9541 aaagataacg attactgaag gatggacaga acacatgaac ttacatgaat atcaggcaaa
     9601 acaacttttt gcccgctatg gcttaccagc accggtgggt tatgcctgta ctactccgcg
     9661 cgaagcagaa gaagccgctt caaaaatcgg tgccggcccg tgggtagtga aatgtcaggt
     9721 tcacgctggt ggccgcggta aagcgggcgg tgtgaaagtt gtaaacagca aagaagacat
     9781 ccgtgctttt gcagaaaact ggctcggcaa gcgtctggta acgtatcaaa cagatgccaa
     9841 tggccaaccg gttaaccaga ttctggttga agcagcgacc gatatcgcta aagagctgta
     9901 tctcggtgcc gttgtagacc gtagttcccg tcgcgtggtc tttatggcct ccaccgaagg
     9961 cggcgtggaa atcgaaaaag tggcggaaga aactccgcac ctgatccata aagttgcgct
    10021 tgatccgctg acaggcccga tgccgtatca gggacgcgag ctggcgttca aactgggtct
    10081 ggaaggtaaa ctggttcagc agttcaccaa aatcttcatg ggcctggcga ccattttcct
    10141 ggagcgcgac ctggcgttga tcgaaatcaa cccgctggtc atcaccaaac agggcgatct
    10201 gatttgcctc gacggcaaac tgggcgctga cggcaacgca ctgttccgcc agcctgatct
    10261 gcgcgaaatg cgtgaccagt cgcaggaaga tccgcgtgaa gcacaggctg cacagtggga
    10321 actgaactac gttgcgctgg acggtaacat cggttgtatg gttaacggcg caggtctggc
    10381 gatgggtacg atggacatcg ttaaactgca cggcggcgaa ccggctaact tccttgacgt
    10441 tggcggcggc gcaaccaaag aacgtgtaac cgaagcgttc aaaatcatcc tctctgacga
    10501 caaagtgaaa gccgttctgg ttaacatctt cggcggtatc gttcgttgcg acctgatcgc
    10561 tgacggtatc atcggcgcgg tagcagaagt gggtgttaac gtaccggtcg tggtacgtct
    10621 ggaaggtaac aacgccgaac tcggcgcgaa gaaactggct gacagcggcc tgaatattat
    10681 tgcagcaaaa ggtctgacgg atgcagctca gcaggttgtt gccgcagtgg aggggaaata
    10741 atgtccattt taattgataa aaacaccaag gttatctgcc agggctttac tggtagccag
    10801 gggactttcc actcagaaca ggccattgca tacggcacta aaatggttgg cggcgtaacc
    10861 ccaggtaaag gcggtactac ccacctcggc ctgccggtgt tcaacaccgt gcgtgaagct
    10921 gttgctgcta ctggcgctac cgcttctgtt atctacgtac cagcaccgtt ctgcaaagac
    10981 tccattctgg aagctattga tgcaggcatc aaactgatta tcaccatcac tgaaggcatc
    11041 ccgacgctgg atatgctgac tgtgaaagtg aaactggacg aagccggcgt tcgtatgatc
    11101 ggcccgaact gcccgggcgt tatcactccg ggtgaatgca aaatcggtat ccagccgggt
    11161 cacattcaca aaccgggtaa agtgggtatc gtttcccgtt ccggtacact gacctatgaa
    11221 gcggttaaac agaccacgga ttacggtttc ggtcagtcga cctgtgttgg tatcggcggt
    11281 gacccgatcc ctggctctaa ctttatcgac attctcgaaa tgttcgaaaa agatccgcag
    11341 accgaagcga tcgtaatgat cggtgagatc ggcggtagcg ctgaagaaga agcggcagcg
    11401 tacatcaaag agcatgttac caagccagtt gtgggttaca tcgctggtgt gactgcgccg
    11461 aaaggcaaac gtatgggcca cgcgggtgcc atcattgccg gtgggaaagg gactgcggat
    11521 gagaaattcg ctgctctgga agccgcaggc gtgaaaaccg ttcgcagcct ggcggatatc
    11581 ggtgaagcac tgaaaactgt tctgaaataa atatctgtaa taagaaatag ccctcgccgc
    11641 ttccctctac aggaatggcg aagggctgtc ggtttcgaca tggttggcca tcgtatgatg
    11701 gccttttttg tgcttatcgc gatgattttc gttgcgctat cagggtaaat ttatagtcat
    11761 cggtattaaa agcgttgcgg ctatattcaa acacccgacc atcaactaaa tatccacgcg
    11821 atactttttc aagaatcggc tttgtctggc tgatattaag cagacggctc atctcttcgg
    11881 ttggcatcag aggaatgatt tcctgttcgc tacgatcgat aaccattttc ttcacttctt
    11941 caataaagtg atattttgaa ttttccatga cctgccaggt gagatccggg aacaacgcaa
    12001 gcggcatcca ggtttcttcc agcgccattg gcttttgctt gcgatagcgc acgcgcttca
    12061 catgccacac acgatcctgc gcggtgattt gtagctgttg ctgaagaaaa tcgtcagccg
    12121 gaatcacttc aaatatcagg acttcactgt gtgtatcgac gtgacggtcc gacagttttt
    12181 catcaaaact ggttaactga aaaatatcgt aattgacccg ctcttctttg acgtaagtcc
    12241 cgctgccctg aatgctttcg aggatctgtt gctcgactaa ctggcgcaaa gcctgacgca
    12301 ccgtaacccg gctgacgcca aactctgttt gtaatgccga ttcggtaggt aacgcatcgc
    12361 caggtttaag ctcgccacgc gcaatttgtt cacgaatgcg atcggcaatc tgccggtata
    12421 agggcttgtg tcccattttt agtatctcat taatacgaat ttagccatta tgcccgataa
    12481 attcatcctg taaataatac aaatacaata caaataattt caatcaagtg aaattgatca
    12541 cataatggta ttgttttatc gggcacactg gcgcgactat aaaaacgatc aagtgaggat
    12601 catgatgaat ctgacgactc tgacccaccg cgatgcgttg tgtctgaatg cgcgctttac
    12661 cagccgtgaa gaggccatcc acgcgttgac tcaacgtctt gctgctctgg ggaaaatttc
    12721 cagtactgag caatttctgg aagaagtgta tcgccgtgaa agccttggcc cgaccgcctt
    12781 aggtgaaggg ttggctgtgc cgcatggcaa aactgctgcg gtaaaagaag cggcgtttgc
    12841 ggtcgccaca ctcagcgagc cgcttcagtg ggaaggcgtt gatggcccgg aagcggttga
    12901 tttagtggtg ctgctggcga ttccccccaa tgaagcgggt actacgcata tgcaactgct
    12961 gacagcgctg accacgcgcc tggcggatga tgagattcgg gcgcgtatac agtcggcgac
    13021 gacgcctgat gagttgctct cggcgctgga tgacaaggga ggcacgcaac cttctgcctc
    13081 tttttccaac gcgccaacta tcgtctgcgt aacggcctgt ccggcgggta ttgctcacac
    13141 ctatatggct gcggaatatc tggaaaaagc cggacgcaaa ctcggcgtaa atgtttacgt
    13201 tgaaaaacaa ggcgctaacg gcattgaagg gcgtttaacg gcggatcaac tcaatagtgc
    13261 aaccgcctgt atttttgcgg ctgaagtcgc catcaaggag agtgagcgtt ttaatggcat
    13321 tcccgcgctt tcagtgcctg ttgccgagcc gattcgccat gcagaagcgt tgatccaaca
    13381 agcgcttacc ctcaagcgta gcgatgagac gcgtaccgta cagcaagata cgcaaccggt
    13441 gaaaagtgtc aaaacggagc tgaaacaggc actgttgagc ggaatctctt ttgccgtacc
    13501 gttgattgtc gcggggggca cggtgctggc ggtcgcggta ttactgtcgc aaatcttcgg
    13561 gctacaagat ctgtttaatg aagaaaactc ctggctgtgg atgtaccgca agctgggcgg
    13621 cgggctgctc ggaattttga tggtaccggt gctcgcggcc tataccgcct attctctggc
    13681 agataaaccg gcgttagcgc caggctttgc ggctggactt gccgccaaca tgatcggctc
    13741 cgggtttctc ggcgcggtcg ttggcggatt gatagccggt tacttgatgc gctgggtgaa
    13801 aaatcacttg cgtcttagca gtaaattcaa tggattcctg actttttatc tctacccggt
    13861 gctcggtacg ttgggagcgg gcagtctgat gctgtttgtg gtgggagaac ctgtcgcctg
    13921 gatcaataac tcgcttaccg cctggctgaa cggtctgtca ggaagtaacg cgctgttgct
    13981 gggtgccatt ctcggtttta tgtgttcctt tgaccttgga gggccagtga acaaagccgc
    14041 ttatgcattc tgcctgggcg caatggcgaa cggcgtttac ggcccgtatg ccattttcgc
    14101 ctccgtcaaa atggtttcgg catttaccgt aaccgcttcc acgatgctcg caccgcgcct
    14161 gtttaaagag tttgaaattg agaccgggaa atccacctgg ctgttagggc tggcaggtat
    14221 taccgaaggg gcgatcccga tggcgattga agatccgctg cgggttattg gttcgtttgt
    14281 gctgggctct atggtaacgg gcgctattgt cggtgcgatg aatatcggcc tttcgacacc
    14341 cggtgccggc attttctcgc tctttttact tcatgataat ggcgcgggcg gtgttatggc
    14401 ggcaattggc tggtttggtg cggcattggt gggggctgca atctcgactg caattctcct
    14461 gatctggcgg cgtcacgcgg ttaagcatgg caactatctg actgatggcg taatgccata
    14521 aacaaaaagg aaacgacgat gaaagcggta tctcgcgttc acatcacccc gcatatgcat
    14581 tgggatcggg agtggtattt caccaccgaa gagtcacgca ttctgctggt caataatatg
    14641 gaagagatcc tgtgccgact ggaacaggac aacgaataca aatattacgt actcgacggg
    14701 caaacggcga tcctcgaaga ttatttcgcg gtgaaaccgg aaaacaaaga ccgcgtgaag
    14761 aaacaggtag aagccggcaa gttgattatc ggcccctggt atacccagac cgataccacg
    14821 attgtttctg cggaatccat cgtccgtaat ctgatgtacg gaatgcgtga ctgcctcgcg
    14881 tttggcgagc cgatgaaaat aggttattta ccagattcct ttggcatgtc cgggcaactg
    14941 ccgcatatct acaatggatt tggcattacc cgcaccatgt tctggcgcgg atgttcggag
    15001 cgccacggta ctgataaaac cgagtttttg tggcaaagca gtgacggtag cgaagtgacg
    15061 gcgcaggtgc tgccgctggg ctacgccatc ggtaagtact tacctgccga cgaaaacgga
    15121 ttacgtaaac gcctcgacag ttattttgac gtgctggaaa aagcgtctgt aaccaaagag
    15181 attttgctgc cgaatgggca tgaccagatg ccattgcagc aaaatatctt cgaagtgatg
    15241 gataagctac gtgagatcta ccctcaacgt aagtttgtga tgagccgctt tgaagaggta
    15301 tttgagaaga tcgaagcgca gcgtgaaagt ctggccaccc tgaaaggtga atttattgat
    15361 ggcaaatata tgcgcgtgca tcgcaccatc ggttctacgc gtatggatat caaaattgcc
    15421 cacgcgcgta ttgaaaataa gattgttaat ctgctggaac cgctggcaac actggcctgg
    15481 acgttgggtt ttgaatacca ccacggcttg ctggagaaaa tgtggaaaga gatcttaaaa
    15541 aatcatgccc acgacagtat cggttgttgt tgcagtgaca aagttcatcg cgaaatcgtc
    15601 gcccgcttcg aactggctga agacatggcg gataatctga ttcgtttcta catgcgcaaa
    15661 attgccgaca acatgccgca gagcgacgcc gacaaactcg tcctgtttaa cctgatgccc
    15721 tggccgcgtg aagaagtgat caacaccact gtgcgcctgc gcgccagcca gtttaatttg
    15781 cgggacgatc gcggccagcc tgtgccgtat tttattcgcc atgcccgtga gatcgatcca
    15841 ggcctaatcg atcggcaaat tgttcattat ggcaattatg atccctttat ggagttcgac
    15901 atccagatca accagattgt cccttctatg ggctatcgca cgctttatat tgaagcgaat
    15961 cagcctggca acgtgattgc ggcaaaaagt gacgctgaag ggattctgga aaatgctttc
    16021 tggcagattg cgctcaatga ggatggttct ctgcaactgg tagataaaga cagcggtgtg
    16081 cgctatgacc gggtattgca aattgaagaa agctctgatg atggtgatga atatgactat
    16141 tcacccgcaa aagaagagtg ggtaattacc gcggcgaacg cgaaaccgca atgcgatatt
    16201 attcatgaag cctggcagag cagggctgtt atccgttatg aaatggcggt gccgcgtaat
    16261 ctgcaagaac gcagcgcaag acaatccact ggcagagtag gggtggaaat ggttgtcact
    16321 ttgagagata acagcaggcg cattgatgtg gatatcaacc ttgataacca ggctgacgat
    16381 catcgtcttc gcgtcctggt ccctacacct tttaacaccg acagtgttct ggcagatacc
    16441 cagtttggtt cgctaacgcg ccccgtgaac gacagtgcaa tgaacaactg gcagcaagaa
    16501 ggctggaaag aagcgccggt tccggtatgg aatatgctca actatgctgc cttacaggaa
    16561 gggcgtaacg gcatggctgt ctttagcgaa gggttacgtg aatttgaagt catcggtgaa
    16621 gagaagaaaa cctttgccat tacgttgctg cgtggcgtgg gcttactggg gaaagaagat
    16681 ctgcttttaa ggcctgggcg gccttcggga attaaaatgc cagtcccgga ctcacaacta
    16741 cgtggtctgc tttcttgtcg cctaagttta ttgagttata ccggtacgcc aaccgccgct
    16801 ggtgtagctc agcaggcgcg agcatggctg actccagtac agtgttacaa caaaatccca
    16861 tgggatgcga tgaagctcaa caaagccgga ttcaacgtgc cggaaagtta tagtttgttg
    16921 aaaatgcccc cagtgggatg cctgataagc gcacttaaga aagctgaaga ccgacaagaa
    16981 gtgattttac ggctgtttaa tccggctgaa tcagcaacct gtgatgcgac tgttgctttc
    17041 agtcgcgagg tgatttcttg ctcagaaacg atgatggatg aacacattac caccgaggaa
    17101 aatcaaggtt caaatctatc ggggcctttt ttacccggcc agtcacggac gttcagttac
    17161 cggcttgcct gaatagcaat caaaccgaag ccacatatgc gcggccagat tgttgacaaa
    17221 gggcgctttg ttcatgccgg atacggcatg aacgctttat tcggtctaca aaagcaggca
    17281 aattcaatat attgcagaga tgatgtagac actggcaagc gtagcgcatc aggtaatttt
    17341 gcgtttatct tcactctcaa gccacgtata tgtggctt
//
LOCUS       DAPPNJ010000095        17311 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000095 DAPPNJ010000000
VERSION     DAPPNJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.095"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..275
                     /locus_tag="RPM73_002582"
     CDS             <1..275
                     /locus_tag="RPM73_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDX6520929.1"
                     /translation="KVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKP
                     LRAAKVEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            282..695
                     /gene="tssE"
                     /locus_tag="RPM73_002583"
     CDS             282..695
                     /gene="tssE"
                     /locus_tag="RPM73_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDX6520930.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            699..2549
                     /gene="tssF"
                     /locus_tag="RPM73_002584"
     CDS             699..2549
                     /gene="tssF"
                     /locus_tag="RPM73_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDX6520931.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHHGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            2513..3595
                     /gene="tssG"
                     /locus_tag="RPM73_002585"
     CDS             2513..3595
                     /gene="tssG"
                     /locus_tag="RPM73_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDX6520932.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            3620..4900
                     /gene="tagH"
                     /locus_tag="RPM73_002586"
     CDS             3620..4900
                     /gene="tagH"
                     /locus_tag="RPM73_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDX6520933.1"
                     /translation="MPEEKLQTLSLQVINGSELETGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            4897..5421
                     /gene="tssJ"
                     /locus_tag="RPM73_002587"
     CDS             4897..5421
                     /gene="tssJ"
                     /locus_tag="RPM73_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDX6520934.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            5424..6755
                     /gene="tssK"
                     /locus_tag="RPM73_002588"
     CDS             5424..6755
                     /gene="tssK"
                     /locus_tag="RPM73_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDX6520935.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            6760..7521
                     /gene="icmH"
                     /locus_tag="RPM73_002589"
     CDS             6760..7521
                     /gene="icmH"
                     /locus_tag="RPM73_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDX6520936.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            7530..10289
                     /gene="tssH"
                     /locus_tag="RPM73_002590"
     CDS             7530..10289
                     /gene="tssH"
                     /locus_tag="RPM73_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDX6520937.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLTMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESEQDNTGAKLADEAGSEQPEETAETVSPVQRLAQLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLTTIIDGKLARLDNVLRSRFDADVIIESEVTDEIMSRVTRAEN
                     GARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDALDDESVTE
                     DETDL"
     gene            10286..11029
                     /gene="tagO"
                     /locus_tag="RPM73_002591"
     CDS             10286..11029
                     /gene="tagO"
                     /locus_tag="RPM73_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDX6520938.1"
                     /translation="MSRLRVFRQTGWFIAGLVAGLPASAAPAETSSLAGVEVAVATAT
                     PPDATATLHAMQSCRQETAALERLDCYDRLLAPLSPSGFNGALVKASFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLMVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            11034..12449
                     /gene="tssA"
                     /locus_tag="RPM73_002592"
     CDS             11034..12449
                     /gene="tssA"
                     /locus_tag="RPM73_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDX6520939.1"
                     /translation="MAMDLRDPNVWVSHLLENLPEEKLASALKDDNPDWEYIDGGIVK
                     LGSLAHSQLDIPELQHRGLVLLASESKDFRLLAHLLRTLQHAGDPLLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIDAFSQNAATAQRDALLGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPAPTPAASSSSPQTIATSESGVTHTSTPTPQIA
                     IDSHDDKTWRDTLLKVAAILCERQPESPQGYRLRRHALWLTITSTPQAESDRRTPLAA
                     VSADMVADYQSRLASADMELWQQVEKSVLLAPYWLDGHYISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLANQPGNQSAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            12558..15992
                     /gene="tssM"
                     /locus_tag="RPM73_002593"
     CDS             12558..15992
                     /gene="tssM"
                     /locus_tag="RPM73_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDX6520940.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            16003..>17311
                     /locus_tag="RPM73_002594"
     CDS             16003..>17311
                     /locus_tag="RPM73_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDX6520941.1"
                     /translation="MDSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYVPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKN"
BASE COUNT         3972 a         4843 c         4881 g         3615 t
ORIGIN      
        1 tcaaggtgtt gcagcgtgaa caactggggt catggaaaga aaggagcgac ttagaaagag
       61 agcttaatac ctggatccgt cagtatgttg ccgaccagga aaacccgcct gcagacgtgc
      121 gcagccgcaa acctctgcgc gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg
      181 gctggtacca ggttgcgcta agcgtgaggc ctcattttaa atttatgggg gcaaattttg
      241 agctttccct ggttggccgg ttagacaggg agtaacacac catgataccg gatcgggaaa
      301 gaaccacagg ttctcttttt gagcgtatgg aagcctcttc ggccagaaac cggcagggag
      361 gcagtattca ttcgctcagg caatccattc gccagaacct acgaaacatt cttaataccc
      421 ggtccggtag ttgccggggt gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa
      481 acttccgcga atccatgagt cgggccatcg agcaatgtat agaacgctat gaaccccgga
      541 tctcacacgc tgaagtgcag gcagtggttt catccgcctc cagcccgctg gatatgacgt
      601 ttcatattac agcctgggtg acatttaacg aaacgcatga ggtgcttgaa tttgatatgg
      661 caccgaatgg cagccagcat taccgggtgg actgacaaat ggaatttgaa gaacgctatt
      721 tccgggaaga actcgattac ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc
      781 atctggcccg cttcctggcc gaaaaagatg cggatccgga tattgaacgc ctgctggaag
      841 gggtggcttt tcttaccggc aatctccgcc agaaaattga ggatgaattc ccagaactga
      901 cgcacgggct tattaagatg ctatggccta attacctgcg tccggttccg gcaatgaccc
      961 ttattgaata tacgccggat atggataagt cttctgtacc ggtgttaatc ccccgtaatg
     1021 agcagtttac aaccaacgcc ggggaaatca gagttgatga agtgctgccc tctgatgcta
     1081 aaaaggagga gccgcctccc tgtaccttca ctctctgccg ggatatctgg ctgctgcccg
     1141 ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa tggtgttatc aacatcacct
     1201 tttcggtcgc accgggaacg gacttccgca cgctggatct gaacaaactt cgcttctggc
     1261 tcggcaatga cgacaactat acccgtgacc agctttattt atggttctgc gaatacttgc
     1321 agggtgccga cctgactgtg ggtgaacagc atcttcgcct gcctgagttt atgctaaaag
     1381 ctgtcggttt tgagccgcag gatgccatgc tgccctggcc gaaaaacgtc cacagcggct
     1441 accggatcct tcaggagtat ttctgttacc ccgatgcgtt tctctttttt gatctttgtg
     1501 gttgtccggc tttgcctgac ggattgcagg cggaattctt taccctgcaa ctgcgttttt
     1561 cgcgcccttt gcccgtggac atccggctgc gccgcgattc cctgcgcctg tattgcgcac
     1621 ctgccattaa tttatttatc caccatgcag aagccatcac gctggacaac cggcgggcag
     1681 actatccgct ggttcccagc cgccattacc cacaacatta cgatgtattt tccgttaaca
     1741 gtgtggtgag ccaggtccag gatatgttca ggaaaaaaga tctggggcgt cctgtttcga
     1801 cgcaggccgc gcgccagtgg ccagcctttg aaagtttcag ccatcagatg gaatacagcc
     1861 ggaagcggga agtggtgtac tggcatcacc ggaccaaaac atccctgttc catcacggct
     1921 ttgatcatac ccttgccttt atacatgctg atggcagtta tccgtcagac gaatctctgc
     1981 tcagtaatga agtggtttcg gtatcgctga cctgtaccaa ccgtgagctt ccgtcacaaa
     2041 ttcgttccgg cgatatcacc ggcacaaccg gtaaaaatgc agctgttgct tcatttcgca
     2101 acattacccg cccgacgcaa ccactctggc cggtcattga tggcagcctg cactggtccc
     2161 tactctccgc catgaacctg aattatctgt cattactgga tacggacgcg ctgaagcagg
     2221 tcatcgccaa ctttgatcgc cacgcaatcc atcatccgca gacggcacgg ctgtcacaac
     2281 aaaagctgga tgccattgag cgtctggaga cccgccccgt tgatcgcctg tttacgggta
     2341 ttcccgtccg gggactggcc tccacgctgt atctgcaccc ggagccgttt gtctgtgaag
     2401 gggaaatgta tctgctcggt acggtgcttt cgcattttct gtcgctgtac gccagcgtta
     2461 actcattcca catgctgacc gttgtgaaca cagaaagcca ggagacatgg aaatggacgg
     2521 aaagaatcgg gcagcatcct cttatctgag tccgggaaat cccccggcag ataaagagca
     2581 gaatgatccg ctggcacagg ttttccataa tgcctgctct tacaattttt ttgcgatggc
     2641 ggagctgttg caccgcctgg caaagggtga aaaggggacg ccagaattat ccctgcgtga
     2701 cgatccggca caggaaaccc tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag
     2761 tgatatcagt gcgctgaaac gggatacatc aggggcattc aggatgacca ccacctttat
     2821 ggggttacag gggagccagt cgcctctccc cggttattac ctcgatcacc tggcctggaa
     2881 agcggtacat gaacaaagtc cggtaggtga ttttctggat atgttcagcc accgcctgac
     2941 ccagtttgtc tggcatatct ggcgtaagta ccgctatcac atcagttttc gtaacggcgg
     3001 tgtggatgcg ttctcgcagc gcatgtactc ccttgtcggg ctgggccacc gccagcttcg
     3061 cgataagctg gcgattaatc acagcaaaat gctggcgtat tccgggatcc tggcaaatcc
     3121 gggacgatcc ccggaaataa tctgtggcct ggtgtcccac tgctttgatt tgtcagaagt
     3181 gacgctgcaa aactggcaac gtcgcaaggt ggatattgaa ccggatcagc aaaatagtct
     3241 ggggagctat tctcttaaaa atggcgaaaa actggcggga agatcggtac tgggcaactt
     3301 tgttctgggt acacgagtgc ccgatctcag cggaaaattc cagctcagta ttaccagcct
     3361 gacccgaaaa cagttcctct cttttctgcc gtccggcgaa aacttcctgc cactgacgat
     3421 gttcgtgtcc ttcatcctgc gcgatcagct tgcctgggac ttacatctgg ggctggcgcc
     3481 ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt gcgctgctgg gctggaccag
     3541 cttcctcggc acaccggaag aacgaccctc agtcaccatc agggtacggt cgtaaattat
     3601 gataatcacg gagcagaata tgccggaaga gaaactacag acgctttcat tgcaggtcat
     3661 taacggcagt gagctggaaa ccggacgggc ggcgcgctgt ctgttcacac agcagggaaa
     3721 tgtcggccat ggccccgaat gccactggtc ggtacaggat cgtcagcaga gcattccggc
     3781 ccaggctttt accgttatcc tgcacgatgg cacattttgt ctgcgcccgc agacggcaca
     3841 actgtggctg aatcaggcaa aagtcacagc aacatcagac ctaatacagt tgcgccaggg
     3901 cgatgagatc cagatcggac ggctgatggt gagggttcat ctgaaccggg gagatattcc
     3961 ccattacgat gaggaaatgg ccactcccga aaccatcgtt accaatcgcg atatgctcac
     4021 ggataccctg ctatcaacgg agggtacgcc acactatccg ggaatgactc accggcacca
     4081 gcttgcagac accgtggtaa acggtttttc tgccgatcca ctccaggcac ttcagtccga
     4141 aagcctgatt accacgggcg atccgctttc aggcattgcg gctgtccggc catcggcacc
     4201 gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc tgccgcccat
     4261 ttatgccagc cccggcgatc gtaatgacga cgtctctgcg gcagaaatgg cgcaacgcca
     4321 ccttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga gcaattccga
     4381 cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa taaaaggtct
     4441 gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc cgctggaaga
     4501 taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt ttgccgaagg
     4561 taaaagcccg gtacatttgg cggctcccgc cgccgtcagt gaaagcctgc gcaatgtccg
     4621 ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc tgctggatgc
     4681 tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc atgaactgcg
     4741 ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg aagaactggc
     4801 ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc aggtctatga
     4861 ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc gtttctggcc
     4921 tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat cagtgaggtg
     4981 atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc
     5041 accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc cgccgtggat
     5101 gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta tgaccagata
     5161 gccagtacgc cgctaccgga tgtgctgggt aaaaactata tcgaccacca ggatttcaac
     5221 ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa aacgcagttt
     5281 attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca aatcgagacg
     5341 gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa
     5401 atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg tggcaggaag
     5461 ggatgctgat gcgcccacac catttccagc aacagcagcg ttacaacgac tacctggata
     5521 accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc accctcaaca
     5581 atgaactgct ggcgcagggt aagatcatga ttgacagcgc gtcaggcaca ctgcccgacg
     5641 gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac ccgcaaaatt
     5701 ttccggacga aagaagccgc aatatctacc tcgctctgcc ggtcgccagt gatgtgagaa
     5761 atgaaatcag cgacggacgg cgaatcgggc gttaccggct gaattatgcc gatgtccggg
     5821 atttgcattc agaagaaggt gacgcgcgaa cattgacgct gggtcaactg acgccgcgca
     5881 ttatgagcgg tgcagaagat atgagcgcct atattacgct cccgctttgc cgtatcagcg
     5941 atcgccatgc cgatggctcc ctgacgttgg atgacgattt tatcccctcc tgccagaata
     6001 ttcaggtcag taagaaactg cgtgtttatc tcaaagaggt acagggggcc attggcggac
     6061 gggcaagcga tctggcaaac cgtattggct ctccggcgca gagcggcatc gcggatgtgg
     6121 cggaatttat gatgttgcag ttacttaacc gtaaccagac ccggtttacc catcgcgccc
     6181 gccgatccca gctccacccg gaagatttct accttgatct tgctgggttg ctgggtgaac
     6241 tgatgacctt taccgagccg tcgcgcctgc cctgcccgct tgatgtgtat gatcatcatg
     6301 atctgaccaa aacatttaaa acactgttac cggaagtcaa acgggcgcta cataccgtac
     6361 tgtcgccaag agcggtcaat ctgccgctgc atctgcgtga cggtatctgg caggccgata
     6421 tccatgacac agaactgctg caatctgcca cctttgtgct ggccgtggcg gcaaacgtgc
     6481 cggtcgatca gatccagcgt cagtttatcc agcagtcgaa aatttcctcg ccggaaaaaa
     6541 tccgcaatat ggtcagtgtg cagatccccg gtattccatt gcgtgccctt atggtggctc
     6601 cccgccagct tccttatcat tccgggttca gctatttcga actcgacaag agcggacagg
     6661 cctggacaga aatggctgcc gccggggccg ttgcactgca tgtatccggc agtttcccgg
     6721 atctgaacat gcaactgtgg gcgataagag ggtaagacga tggatgaggg gtcactgtct
     6781 ctgccaccat ttaccggata cgacgaaaaa agccagcgca actaccatct cgcgcttcgt
     6841 ggcaacagcc tgaatccgat gatcgacgcg gccacgccgc tactcggtat ggtaatgcgc
     6901 ctttcaacca tgaacagcca gacgatgccg gagcatcttt ttgctcaggt ggtgacggat
     6961 gttcaggccg tggagcaact gcttcaggag cagggctacg aaccgggcgt gattatctcg
     7021 tttcgctaca tcctctgcac gtttattgat gaagcggcgc tcggtaacgg ctggtcaaac
     7081 aagaacgagt ggatcaagca gtcattgctg gtccatttcc acaacgaagc ctggggtgga
     7141 gagaaagttt tcatcctgct ggagcgcctg atccgtgaac ccaaacgtta ccaggatctg
     7201 ctggaatttc tctggatctg tttttctctg ggatttcgcg ggcgctacaa agtggcagca
     7261 caggatcagg gggaatttga gcagatctac cgccgtctgt accacgttct gcataaactg
     7321 cgcggcgatg cccctttccc gctgttacac caggacaaaa aaacccaggg cgggcgttat
     7381 cagctcatca gtcgcctgac tgttaaacat attttttgtg gtggcgttgt cgtgctggcg
     7441 ttgttttacc tgttctatct gttgcggctg gacagccaga cgcaggacat cctgcaccag
     7501 ctaaacaaat tacttcgata aggacgttta tgatccagat tgatcttccc acgctggtaa
     7561 aacggctgaa cctgttctcc cgccaggcgc tggagatggc cgcctctgaa tgtatgagtc
     7621 agcaggcagc ggaaattacc gtcagccatg tgctcattca gatgctcacc atgccacgca
     7681 gtgacctgcg ggttattacc cgccagggcg atattggcat ggaagagttg cgccaggcgc
     7741 tgacggtaga gaactacaca accgcccgtt ctgcggacag ctacccggcg ttttccccga
     7801 tgctggttga gtggctgaaa gagggctggc tgctggcgtc ggctgagatg cagcacagcg
     7861 aactgcgcgg cggcgtgttg ctgctggccc tgctgcattc gccgctgcgt tatataccgc
     7921 ctgctgccgc ccggctgttg accggcatta accgtgaccg tctgcaacag gactttgtgc
     7981 aatggacaca agagtcggcg gaatcagtcg tgccggatgc agacggtaaa ggcgcaggca
     8041 cactgacgga cgctgctgac accctgcttg cccgctacgc caaaaacatg accgcagacg
     8101 cccgtaacgg caggcttgac ccggtactgt gccgcgacca cgaaatcgac ctgatgatcg
     8161 acattctctg ccgccgccgc aagaacaacc cggtggtggt gggcgaagca ggcgtgggta
     8221 aaagtgcctt aatcgaaggg ctggcgctgc gcatcgtggc aggccaggtg ccggacaaac
     8281 tgaaaaacac cgatatcatg acccttgact tgggcgcatt gcaggccgga gcgtcggtga
     8341 agggtgaatt cgaaaaacgt ttcaaagggc tgatggcgga ggtcatttcc tccccggtgc
     8401 cggtcattct gtttatcgac gaagcacata ccctgattgg cgcgggcaac cagcagggcg
     8461 ggctggatat ctccaacctg ctcaaaccgg cgctggcgcg cggcgagctg aaaaccatcg
     8521 ccgccaccac ctggagcgag tacaaaaaat acttcgaaaa agatgccgcc ctgtcgcgcc
     8581 gcttccagtt ggtgaaggtc agcgaaccca acgctgccga agccaccatt attctgcgcg
     8641 gtctgtcggc ggtctatgaa cagtctcacg gcgtgctgat tgatgatgac gccttgcagg
     8701 ccgctgcgac attaagcgag cgttatctct ccgggcgtca gttaccggac aaagcgattg
     8761 atgtgctgga taccgcctgc gcccgtgtgg ccatcaacct gtcgtcgccg ccgaagcaaa
     8821 tctcggcgct gaccactctg agccaccagc aggaggcgga aattcgccag cttgagcgcg
     8881 agcttcgcat cggactgcgt accgacacat cacggatgac cgaggtgctg gtgcagtatg
     8941 atgaaacgct gacggcgctg gatgaactgg aagcggcctg gcaccagcag cagacgctgg
     9001 tccgggagat tattgcgctg cgccagcagt tactgggcgt ggcagaggac gatgcggcgc
     9061 cgttgccgga cgcagatacc gtggaggata cgcagccaga gtcagaacag gataataccg
     9121 gtgctaaact ggctgatgaa gctggcagcg aacagccgga agagaccgca gaaacagttt
     9181 ccccggtgca gcgactggca cagctcactg ccgaactgga cgccctgcat aacgaccggt
     9241 tgctggtctc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc gaatggaccg
     9301 gcgtaccgct taaccgcctg tcacagaatg agatgtcggt catcaccgac ctgccggtat
     9361 ggctgggtga caccatcaaa ggccaggacc tggcgattgc cagcctgcat aaacacctgc
     9421 tgactgcacg cgccgacctg cgtcgtccgg gacgcccact cggcgcgttt ctgctggccg
     9481 gtcccagcgg cgtgggtaaa accgaaaccg tcctgcaact ggcagaactg ctctacggcg
     9541 gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggaaaaacac accgtctcgc
     9601 ggctgattgg ctcccctccg ggctatgtcg gctatggcga aggcggcgta ctgaccgaag
     9661 cgattcgcca gaaaccgtac tcggtggtgc tgcttgatga agtggaaaaa gcgcacccgg
     9721 atgtcctcaa cctgttctac caggcgttcg acaagggcga gatggcagac ggcgaaggcc
     9781 gactgattga ctgtaagaat atcgttttct tcctcacctc caaccttggt taccaggtga
     9841 ttgttgaaca cgcggatgac ccggaaacca tgcaggaagt gctgtatccg gtgctggcgg
     9901 acttctttaa accagccctg ctggcgcgta tggaagtggt gccgtacctg cctctgtcga
     9961 aagagacgct caccaccatt atcgacggga aactggcccg cctggataac gtgctgcgca
    10021 gccgctttga tgcggacgtg attattgagt cggaagtgac ggacgagatc atgagccgcg
    10081 tcacccgcgc ggaaaacggc gcaaggatgc tggagtccgt cattgacggc gacatgctgc
    10141 ccccgctctc gctgctgctg ttgcagaaaa tggcggccaa cacggcgatt gcccgcatcc
    10201 gcctgtcggc ggcagacggc gcgttcacgg cagacgtgga agatgctctg gacgacgagt
    10261 ctgtcacaga ggatgaaacg gatttatgag caggttgcgc gttttccggc agacaggatg
    10321 gtttatcgca ggcctggtgg cgggcttacc cgccagtgcc gcgcccgcag aaacatcgtc
    10381 actggccggt gtggaggtag ccgtcgccac tgcgaccccg ccggatgcga ctgccacgtt
    10441 acatgccatg cagtcctgtc ggcaggagac ggcggcgctg gagcggcttg actgttacga
    10501 ccgcctgctg gcaccactgt ccccgtcagg atttaacggg gcactggtca aagccagctt
    10561 tgtgggcgaa gcctggaccc gcgctacgga gcaggaaaaa cgccgccagg gcaacaccac
    10621 agagctgctg gtgacgcagg tgccggggga acgcccgacg gtggtgataa ccaccccggc
    10681 tatcggccat gttccgccgc gcccggtgct gatgttcagt tgcgtggaca atatcacccg
    10741 gatgcaggtg gcgctgatgc accctctgga tgttcatgat atcgccgtca ccctgaatgc
    10801 cgacagccgg gcattgcgca gccactggtt tgtgcgcgag aacggcactc tgctggaatc
    10861 cagccggggg ctgtccggga ttgatgaaat taagcaactg ttcggcgcaa aaacgctgat
    10921 ggtggatacc ggcgcagaca acgccgccgg aaagctgacc tttaacattg acgggctggc
    10981 aagggcgatt gctcccctgc gcgatgcctg ccactgggcg ggagaataaa aatatggcaa
    11041 tggacttacg cgatccgaat gtctgggtat cgcacctgct ggaaaacctg ccggaagaga
    11101 aactggcatc ggcgctgaag gacgacaacc cggactggga gtatatcgac ggtgggattg
    11161 tcaaactggg atcgctggcg cacagccagc tggatatccc tgaactccag caccggggac
    11221 tggtccttct ggcttcggag agtaaggatt ttcgcctgct ggcgcatctg ctgagaaccc
    11281 tgcaacatgc cggtgatccg ctcctggcct tgcgcctgct ggcgctgtat gtggagcatt
    11341 actgggcggt agccgcgccg cagaatatgg cacataaaaa acgctttgcc agccaggtta
    11401 ttaaacgctt cgaaacaggt attgacgctt tttcacagaa cgccgccaca gcgcagcgcg
    11461 atgcgctgct aggtgaactg gcgaaactgg cgcaatgctg gcagtcacat aatgccccgg
    11521 aactagcaca ggctactgat gatctttttg ccctttacca gcgtgcgttt aatcgtgcgg
    11581 caccggctcc agcccccact ccggcggcct ccagtagttc accacagacg atcgccacgt
    11641 ctgaaagcgg cgtgacgcat accagtactc cgactccaca gatcgccatc gacagtcacg
    11701 acgacaaaac ctggcgcgac acactgttaa aagtggcggc cattttatgc gagcgccaac
    11761 cggaatcgcc gcagggctat cgcctgcgtc gccatgccct gtggctaacc atcaccagta
    11821 cgccccaggc ggaaagcgat agacgtactc cactggctgc ggtctctgcc gatatggtgg
    11881 cggattacca gtcccggctt gccagcgcgg atatggagct gtggcagcag gtggaaaaaa
    11941 gcgtactgct ggcaccctac tggctggacg gtcactacat ttctgcacag acggcgcacc
    12001 gtcttggtta taccgctgta gccgatgcca tccgcgatga agtcgtttcc tttcttggcc
    12061 gtctgcccgc gctcgctgat cttctgttta acgaccacac accgtttgtt tctgaaaaaa
    12121 cgaagcaatg gcttgcaaat cagccaggta accagtccgc gccagtcata caggtgagta
    12181 atgaagatat tcaggctgcg cggttgtgtt ttgatgagca ggggctggaa gccgccttac
    12241 ggtatctgga tacgttaccc gccggtgaac cccgcgacca gttccaccgg cagtattttg
    12301 gcgcgcagct tatggaagag gcggggctgg tacagcttgc gcaacagcag tacaggatgt
    12361 tgttcaggat gggattgcag atgatggtgg cagactggga gccgtccctg cttgagcagc
    12421 tagaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg ttcagattac
    12481 caacaccccg actattcagc ggactcaaat cagccctgcg cccggcgatg ccccgattta
    12541 aagtctctgc attctggctg ctgatactgg cgtggatttt cctgctggtt tggatctggt
    12601 ggaaaggccc gacgtggacg ctgtatgaag agcagtggct caaaccactg gcgaaccgct
    12661 ggctggcaac ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc cgggtaatga
    12721 agcgcctgca acagcttgaa aaacagcaaa aacaacagcg cgaggaagcc attgatccgc
    12781 tcagtgtgga actgaacgcc cagcagcgtt acctcgaccg ctggctgctg cgcctgcaac
    12841 gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc atcggcccgg
    12901 cgggcagtgg caaaacgaca ctgctgcgcg aggggtttcc gtccgacatt atttatgccc
    12961 cggagggcgc acgcggcgca gaacaacgcc tgtacctcac gccccatgtc ggtaaacagg
    13021 cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat atcctgcatc
    13081 gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc cagccgctga
    13141 acgggattat tctgacactc gatttacccg atctgctgac cgcagacaaa cgccgccgcg
    13201 agcatctgtt acagacgctg cgcagccgct tacaggatat acgccagcat cttcactgcc
    13261 agttaccggt ttacgtggta ctgacccggc ttgatttatt acagggtttt gccgccctgt
    13321 tccagtcact caacagacag gatcgcgatg cgattctggg cgtcacgttc acccgccgtg
    13381 cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca tgggtggatc
    13441 gaatgaatct ggcgttgccg gatctgatgg tcgctcagac tcacacccgc gcgtctttat
    13501 tcagtttttc ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg cttgagggtc
    13561 tgcttgatgg cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc tcttcgcttc
    13621 agcgtggaca gatggatgat atattcaccc agtctgccgc ccgccagtac cggctgggca
    13681 ataacccact ggcgtcctgg cccctggtgg acaccgcgcc ttatttcacc cgcagcctgt
    13741 tcccgcaggc attactcgca gagcctaatc tggcaacaga gagccgcgcc tggctgatac
    13801 gttcccgtcg ccgcctgacg gttttctctg ccacaggcgg cgtggcagca ctgctgctca
    13861 tcaccggctg gcatcactat tacaacggta actatcagtc cggcatcacc gtgcttaagc
    13921 aggccaaagc ctttatggac gtgccgcctc cgcaggggga agatgacttt ggcaacctgc
    13981 aactgccgct cctgaacccg gtacgcgatg ccacactggc ctatggcgac tggggcgacc
    14041 gcagccgtct ggccgatatg ggactgtacc agggacgacg tatcgggcct tatgtggaac
    14101 agacctatct gcaactgctg gagcaacgtt acctgccctc gctgtttaac gggctggtca
    14161 aagcgatgaa cgccgcgccg ccggagagtg aagaaaaact cgcggtgctg cgcgtaatgc
    14221 gaatgcttga ggacaaaagc ggacgtaaca atgaggtggt gaagcagtat atggcaaaac
    14281 gctggagcga aaaattccac ggccagcgcg atatccaggc acaactgatg tcccatcttg
    14341 actacgcgct ggctcatact gactggcatg cagagcgtca ggcgggcgac ggtgacgcta
    14401 tcagccgttg gacgccatat gacaagcccg tggtatcagc acagaaagaa ctgagcaaac
    14461 tgcctgtcta ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc gttcttcctg
    14521 ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt acatctgccg
    14581 atgacaacaa actggttgtt ccacagtttc ttacccgtta cggcctgcaa agctattttg
    14641 taaaacagcg cgatgaactg gttgaactga cggcaatgga ttcctgggta ctcaacctca
    14701 cccgcagtgt gaaatacagt gacgccgacc gcgcggaaat ccagcgccag ttgaccgagc
    14761 agtatatcag cgactacacc gctacctggc gagccgggat ggacaatctg aatatccgca
    14821 attttgagtc cattggacaa ctgaccgggg cgctggagca ggttatcagc ggcgaccagc
    14881 ctttgcagcg ggcgctgact gtgttgcgtg acaacacaca gccaggcgtc ttttctgaaa
    14941 aactttctgc caaagaacgg gatgaggctc tggcagaacc ggactaccag ttgctcaccc
    15001 gcctcgggca tgaatttgcc ccggaaaaca gtactctggc agtacagaaa gacaaagaaa
    15061 gcacgatgca ggccgtgtat cagcaactca ccgagttgca ccgctatctg ctggcaattc
    15121 agaacgcgcc tgtaccaggg aaatcggcgc tgaaagccgt gcagttgcgg cttgatcaga
    15181 acagcagcga tccgatattc gccacccgcc agatggcaaa gacactgcct gctccgctca
    15241 accgctgggt tggcagactg gctgaccagg cctggcatgt ggtgatggtg gaggccgttc
    15301 actatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag caactggcaa
    15361 ataactatcc gtttaatccg cgttctgcac aggatgcctc actggatgcc ttcgaacgct
    15421 tctttaaacc ggatggcata ctggatacct tctaccagca gaacctgaag ctgtttatcg
    15481 acaatgacct gagtctggag gatggcgata acagcgtcat tattcgcgaa gatattattg
    15541 cgcaactgaa aaccgcgcag aaaatccgtg acatcttctt cagtaaacag aacggtctgg
    15601 gaacatcctt tgccgtggaa acggtatcgc tctccggcaa caaacgccgc agtgtactga
    15661 accttgacgg ccagttagtc gattacagcc aggggcgcaa ctataccgcc catctggtct
    15721 ggcctaacaa tatgcgcgaa ggcaacgaaa gcaaactgac gctcatcggc accagcggca
    15781 acgcaccgcg cagtatcagc ttcagcgggc cgtgggcgca gttccgcctt ttcggggccg
    15841 gacaactgac cggagttcag gatggcaact ttaccgtgcg ctttagcgtg gacgctggcg
    15901 cgatgaccta ccgtgtgcat accgacactg aggataaccc attcagcggt gggttattca
    15961 gccagtttgg tctgtcagac acactgtact gaggaggcag ggatggatag taacggactg
    16021 acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag cgccatccgc
    16081 gaggaaataa acaaagccag ccacccgtca cagcctgagc tgaactggaa actggtggag
    16141 tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc cacctactat
    16201 acgcttgccc ggacacggac ccagggactg gcgggattct gcgaaggtgc ggaactgctg
    16261 gcggcaatgg taagccacga ctgggataag ttctggccgc agggcggccc ggcgcgtact
    16321 gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca aatctccttt
    16381 gcggaatccg acctaccact gatctaccgc acagagcggg cattgcagct tatctgcgat
    16441 aagctccagc aggtggaact gaagcgcgtt ccgcgcgtgg agaatctgct ctattttatg
    16501 cagaacacgc gtaaacggct tgaaccccag ctgaagagta acactgagaa cgccgcacag
    16561 accacggtca gaacgctgat ttatgtcccg gaaacacagg catcttccac accagaagcg
    16621 gtagtgcctc ccctgcccgg cctacctgag atgaaagtgg aagtgcgcag tctgacagag
    16681 aatcccccac aggccagtgt gataaagcaa ggcagtacgg taagagggtt tatcgcaggg
    16741 atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta tccggtgcag
    16801 cagcaactgt tacaggttaa cgacaccgct cagggcgcag caacggtgtg gatggcctca
    16861 cctgaactcg aaaactatga gcgcaggctg caacaacttc ttgatacctc cccggtacag
    16921 ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc ggaaagcctg
    16981 caacagcaac aggcctcgac acaatggaat gaggcactca aaacccgcgc acagagtagc
    17041 ccgcagttgc gtggctggtt gcagacccgc caggacttac atgcttttgc agatctagtg
    17101 atgcagcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat ctggcaggcg
    17161 gagcggggac tggggcagga aacacccgtt gagtctctgt tgacgcagta ccatgatgcc
    17221 cgtgcgcaga agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg actcgaaggc
    17281 gtgttaagcc gctggctgct gctgaagaat a
//
LOCUS       DAPPNJ010000096        17295 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000096 DAPPNJ010000000
VERSION     DAPPNJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.096"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            226..534
                     /locus_tag="RPM73_002595"
     CDS             226..534
                     /locus_tag="RPM73_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDX6520942.1"
                     /translation="MTVIVYGKSTFAGNAKTRRHERRRKLAIERDAICNIIDSIFGTD
                     SEEPVQEAPRKRLSISEKAISLGIISKSNTDANRGSVCLPEVALYAAGYRRQKLITAR
                     "
     gene            537..815
                     /locus_tag="RPM73_002596"
     CDS             537..815
                     /locus_tag="RPM73_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520943.1"
                     /translation="MKPSITIKPARDNSRNRRREARKAERAEKENKSREERKAEILEK
                     GGLAAVRGEKAAELAVAHEKTEIDNNSGGDAMFKVVNHAHQRNPKKKW"
     gene            873..1238
                     /locus_tag="RPM73_002597"
     CDS             873..1238
                     /locus_tag="RPM73_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520944.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVIHQLLMWHAV
                     SSAGIQAICVLVIIACVYLMIFAWNKGDDADIVLLSLLVTSGIAITSIVVFFNYFDWL
                     KIWLAPKLYLIEYAASLVK"
     gene            1427..2395
                     /locus_tag="RPM73_002598"
     CDS             1427..2395
                     /locus_tag="RPM73_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HDX6520945.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKSKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKEEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            2420..2551
                     /locus_tag="RPM73_002599"
     CDS             2420..2551
                     /locus_tag="RPM73_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDX6520946.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            2536..2688
                     /gene="kil"
                     /locus_tag="RPM73_002600"
     CDS             2536..2688
                     /gene="kil"
                     /locus_tag="RPM73_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDX6520947.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            2764..2934
                     /locus_tag="RPM73_002601"
     CDS             2764..2934
                     /locus_tag="RPM73_002601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520948.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            2943..3650
                     /locus_tag="RPM73_002602"
     CDS             2943..3650
                     /locus_tag="RPM73_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDX6520949.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEDAQA
                     KCVQVMNIRVNELKQAA"
     gene            3651..4166
                     /gene="ssb"
                     /locus_tag="RPM73_002603"
     CDS             3651..4166
                     /gene="ssb"
                     /locus_tag="RPM73_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDX6520950.1"
                     /translation="MASRGVNKVIIIGRLGHDPEIRYSPSGTAFANLTVATSEQWRDK
                     QTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEV
                     IVGVGGTMQMLCGKQGSNEQSSPQRNNGQQQRQQSQQQGNHSEPPMDFDDDIPFAPVT
                     LPFPRHAIHAI"
     gene            4175..4723
                     /locus_tag="RPM73_002604"
     CDS             4175..4723
                     /locus_tag="RPM73_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDX6520951.1"
                     /translation="MNHLMVDLETMGNGPYAPVISIGAVFFDPNTGETGEEFSVNISL
                     ESSMRYRARPDASTILWWMEQSEEARKSLTSNTQELSTALSWLSEFIIKNANHKFVQV
                     WGNGASFDCVILRNSYSLTGQPVPWQWWNDRDVRTIVELGKVIGFDPKRDMPFKGTRH
                     NALDDAIHQAKYVSAIWKKLAK"
     gene            4740..5036
                     /locus_tag="RPM73_002605"
     CDS             4740..5036
                     /locus_tag="RPM73_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDX6520952.1"
                     /translation="MPAPLYGADDPRRCSGNSISEVLENIKNNLDAFLALPPETKEER
                     KYRRDIQLAEKQEKDRINETSIRPFRKSTYTHFPEYIDPRLRNYRSRYGAISND"
     gene            5047..5211
                     /locus_tag="RPM73_002606"
     CDS             5047..5211
                     /locus_tag="RPM73_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDX6520953.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFGSVNDNKY
                     LNAMWRSGKK"
     gene            5208..5684
                     /locus_tag="RPM73_002607"
     CDS             5208..5684
                     /locus_tag="RPM73_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520954.1"
                     /translation="MKQMSLIEMDGFLKGKCIPRDLKVNETNAEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENTSLKQSEKEFNDFCREEFSEWEDDVTETPAT
                     DTFLDEVRAQAFNDLCSAFVKHANLAGMDDGELVRLKYATDALLHCADQLRKGVHS"
     gene            5681..5914
                     /locus_tag="RPM73_002608"
     CDS             5681..5914
                     /locus_tag="RPM73_002608"
                     /inference="COORDINATES: protein motif:HMM:NF025712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HDX6520955.1"
                     /translation="MSNLLPCPFCGGAAHVASEADHPEYGSGGRFYFVRCGTCRAQSG
                     SKYAAPGNDCAIFYSEVRAEWNQRAKEANSEQD"
     gene            5901..6476
                     /locus_tag="RPM73_002609"
     CDS             5901..6476
                     /locus_tag="RPM73_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDX6520956.1"
                     /translation="MSKIDYQALREKAEKATCGEWSIEYGDGSFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLGELEAAKKRIAELEAE
                     LVSQTYKLNELSGNSPVTPDGWISCSDRMPDSKTAVLVAMEFDRKGDWRMKWATYIPG
                     HPDANDGWIIPGASWKPSHWMPLPEPPQEAK"
     gene            6476..6754
                     /locus_tag="RPM73_002610"
     CDS             6476..6754
                     /locus_tag="RPM73_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HDX6520957.1"
                     /translation="MDSFAKYTIIDWMAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSVIVVTTQSGMTIQIHKPKEEK"
     gene            6909..7076
                     /locus_tag="RPM73_002611"
     CDS             6909..7076
                     /locus_tag="RPM73_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520958.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHKNA"
     gene            7134..7334
                     /gene="torI"
                     /locus_tag="RPM73_002612"
     CDS             7134..7334
                     /gene="torI"
                     /locus_tag="RPM73_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HDX6520959.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(7863..9110)
                     /locus_tag="RPM73_002613"
     CDS             complement(7863..9110)
                     /locus_tag="RPM73_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDX6520960.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(9182..10096)
                     /locus_tag="RPM73_002614"
     CDS             complement(9182..10096)
                     /locus_tag="RPM73_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDX6520961.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            10312..11745
                     /gene="cscA"
                     /locus_tag="RPM73_002615"
     CDS             10312..11745
                     /gene="cscA"
                     /locus_tag="RPM73_002615"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDX6520962.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11753..12811)
                     /locus_tag="RPM73_002616"
     CDS             complement(11753..12811)
                     /locus_tag="RPM73_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX6520963.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            12991..13209
                     /locus_tag="RPM73_002617"
                     /pseudo
     CDS             12991..13209
                     /locus_tag="RPM73_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            13227..14555
                     /gene="dsdA"
                     /locus_tag="RPM73_002618"
     CDS             13227..14555
                     /gene="dsdA"
                     /locus_tag="RPM73_002618"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDX6520964.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14663..16201)
                     /gene="emrY"
                     /locus_tag="RPM73_002619"
     CDS             complement(14663..16201)
                     /gene="emrY"
                     /locus_tag="RPM73_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDX6520965.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(16201..>17295)
                     /gene="emrK"
                     /locus_tag="RPM73_002620"
     CDS             complement(16201..>17295)
                     /gene="emrK"
                     /locus_tag="RPM73_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDX6520966.1"
                     /translation="LFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTV
                     VNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASA
                     RIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMN
                     TPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVP
                     ARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQ
                     NATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTS
                     MPAYTSKALVIDTSPIEKEISNIISHNGQF"
BASE COUNT         5135 a         3940 c         4107 g         4113 t
ORIGIN      
        1 actcaataaa gtacacatcg tactatttag ccatcagcag gaagctggaa gccaaacgga
       61 acagattggc aggctcttta acttcgatgg ggcgctgaca aagcgcaaac agataccaaa
      121 cgagatgggt ttggcggtga tgtgaattgc agctgcaacg acagcaacca gaagatcagc
      181 acctggcgca tcaccaccaa agccatttca catgaggaaa atatcatgac ggtaatcgtg
      241 tacggaaaat caacatttgc aggaaatgcc aaaactcgcc gtcatgagcg gcgcagaaag
      301 ctggctatcg agcgtgatgc tatatgcaac atcatcgatt cgatcttcgg cacagacagt
      361 gaggaacctg ttcaggaagc cccgagaaag cgtttaagca tttctgaaaa agcaatatca
      421 ctcggcatca ttagcaaatc aaatacagat gcgaatagag gaagcgtttg tttgcctgaa
      481 gttgcactgt atgctgctgg ttacagaagg caaaaactaa ttacagcgag gtaaaaatga
      541 agcctagcat aacaatcaag ccagcgagag ataatagcag gaaccgccgc agagaggcca
      601 ggaaggcaga aagagcagag aaggaaaaca agagccggga ggagagaaag gcagagattt
      661 tggaaaaagg aggattggct gccgtgaggg gtgaaaaagc agctgaatta gcagtagctc
      721 acgaaaagac agagattgac aataattctg gtggagatgc aatgtttaaa gttgtcaatc
      781 acgcccatca acgaaacccc aaaaagaaat ggtaatcaac ccgtccaaga cgggtttttt
      841 attgcaagca aaccacttat ttgagatgag atatggaaga acaagcaaac aagattctcg
      901 tagaactact gcaaaaagcc agtaatggaa tagacgcggc tgtttcattt agccaggcac
      961 agattcctga tgtcatccat cagcttttga tgtggcacgc cgtatcatca gctggaattc
     1021 aggctatctg tgtattggtg attatagcgt gtgtttatct gatgattttt gcatggaata
     1081 aaggagatga tgcagatatt gttcttttat cactacttgt cacatcagga atagcgatta
     1141 cttctattgt tgttttcttc aattatttcg actggctgaa aatttggctt gctccaaaac
     1201 tttaccttat cgaatatgca gcatcattgg ttaagtaatt tcaggccgca taatcgtcct
     1261 ttatttttgg cataaacaac agaataaaca cagcactgtg tattcattcc aacgagtgaa
     1321 tacacggagc aatgtcgctc gtaactaaac aggagccgac ttgttctgat tattggaaat
     1381 cttctttgcc ctccagtgtg agggcaattt ttttgacgga ggatatatga gtgaagtaac
     1441 agatttagtt gttattgaaa aagcaaatgc aatgactgta tttcagtctg ccgaccagat
     1501 tgaagaaatc cttcaaaagg ttgaacgtga agttatgtcc tttgtgcctg atatcacaac
     1561 ggcaaagggc agaaaggaga tcgcttctct ggcgtataaa gttgcgcaga cgaaaacata
     1621 tctcgatggt cttggaaaag accttgttgc tgaactgaag gaaattccaa agctaattga
     1681 tgccaaccgc aagacagtgc gtgatcgcct tgatgagctg aaatccaagg cacgccagcc
     1741 tcttactgat tatgaggagg aacaggcacg gattaaagcc gaagaagaag ctaaggcagc
     1801 agctgaagct ctcgcaaagc aaattgagtc tgaccatgaa atagctattt tgatggatcg
     1861 cgaatttgac cgccaaagag aagaggcaag actcaaagcg gagcaggaaa agcgagagca
     1921 tgaagaacgc ttaaaaagag aagctgaaga gaaagccaga gcagaagccg aagcaaaggc
     1981 aaaagccgaa attgaagcag cagcaaggcg agaagcagaa gctaaggccg cagcggaacg
     2041 tgcagagcgt gaacgcattg aagccgagca acgagcacag cgcgaagcaa aagaggcagc
     2101 agaacgagct gaaagagaaa agcaggcggc aattgaagca gaacgccgaa aagcacagga
     2161 ggaggctgaa cgaatccggc gcgaggctga agcaaaagag caagccagaa tagcagaaga
     2221 aaaaagaatc aaggaagaag aagagcgtag agcaaaggat aaagctcacc ggaaagaagt
     2281 aaataacaaa atacttgctg accttatcaa ggttggcgca tcagaagatg ttgctaaaaa
     2341 tatcataaca gccatcgtaa aaggcgaagt attcgcaaca aaaataacct actaataaaa
     2401 ccaacataag gaaccaccca tgatttacgc aatcgcggga ggcgctcgca tgggtgcctt
     2461 ccaattaaat gaatctttac ttgaacgaat cacccgtaaa ttacgtgacg gatggaaaag
     2521 agttgaggtc ttattatgcg caatgaaata gccatcaatc accagatgct tcgtgctgca
     2581 cagaacaaag cagtaatagc cagatttatt ggtgattcaa aaatgtggct tgaagcaaat
     2641 aaagcgatga aatcagctat caaccttccg tggtatcgca ggaaatgagt tttacagata
     2701 actggtcaga cgaagaattc attcgtcaga tgaaagaatt aatcggtaac gaaggagata
     2761 ttcatgtcac ttgcaaccac agtgaaggag agcaagttac agagacgcat gtacacgcag
     2821 caggcgttaa tgtatcgcca gaagggagat cgtgaaggtg ttcgcgtatt tttaaatgcg
     2881 gcaaagactg aagtattaaa tcagcgttat ttccttgggc cgtgtccatt ctgaggtgag
     2941 ttatggattt gaataaattc gatgagccat tcagccctga agatatcgaa tggcgaatac
     3001 agcaaagcgg taaaacacgc gatggcaagg tgtgggctat ggtgctggct tatgtcacga
     3061 accgggcaat catgaaacgc ctggacgatg tttgcggcaa agcaggatgg cgcaatgaat
     3121 accgcgatat tcccaacaac ggcggagttg aatgcggcat atcaatcaag attgattccg
     3181 aatgggtaac caaatgggat gctgctgaaa acacgcaggt agaagccgta aaaggtggtc
     3241 gttcaggtgc aatgaagcgt gctgccgttc agtggggaat cggtcggtat ctgtataacc
     3301 ttgaggaagg tttcgcacaa acatctctcg ataaaaagca ggggtggcac agggcaaaac
     3361 tgaaggatgg aacaggattt tactggctcc ctccatcgct gccgggatgg gcaatcccag
     3421 catcagataa caaaccatca ccagaaaata ccaaccagaa atctccatcg gttgactgcg
     3481 aacaaatcct gaaagacttc agcgattatg cgtcaacaga aactgacaag aaaaaactca
     3541 tcgagcgtta tcagcgtgac tggcaattaa tggctggcaa tgaggatgcg caggctaaat
     3601 gcgttcaggt aatgaacatc agagttaacg aactaaaaca ggcggcataa atggcaagca
     3661 gaggcgtaaa taaggtgatc attattggtc gccttgggca tgatccagaa atcagatatt
     3721 caccatcagg aacggcattt gcaaacctta cagttgctac gtcagaacaa tggcgtgata
     3781 agcaaactgg agagcaaaag gagcagacgg agtggcaccg tgtggtaatg agcgggaaat
     3841 tggcagaaat tgccagcgaa tatctgcgaa aaggctctga ggtttatctt gaaggcaaat
     3901 tgcggacaag aaaatggcag gatcaaagcg gacaggatcg gttcactacc gaagttatcg
     3961 tgggcgttgg tggaaccatg caaatgcttt gtggcaagca aggaagcaat gaacagtctt
     4021 cacctcagcg aaataacggc cagcaacaaa gacagcaatc tcagcagcag gggaatcaca
     4081 gcgaaccacc tatggatttt gacgacgata taccctttgc gccagtaact ctccccttcc
     4141 ctcgtcacgc tattcacgca atttaaggac ttacatgaat cacttaatgg ttgaccttga
     4201 aacaatgggc aacgggccat acgcgccagt tatttctatt ggggcggtat tctttgaccc
     4261 gaataccgga gaaacaggag aagagttctc ggtaaatatc tcgcttgagt catcaatgcg
     4321 atatcgagcg cgtcctgacg cttcaacgat tttatggtgg atggaacaga gtgaagaagc
     4381 cagaaaatcg ctaaccagca acactcagga gctttcaacg gctctttcat ggttatctga
     4441 attcatcata aagaacgcta accacaaatt cgttcaggtt tgggggaatg gagcatcatt
     4501 tgactgcgtt attctccgca acagttattc gctgacaggg cagccagttc cgtggcagtg
     4561 gtggaatgac cgcgacgtaa gaacaatcgt cgaacttggg aaggtaatag gattcgatcc
     4621 taagcgagat atgccattca aaggaactcg ccacaacgcg cttgatgatg ccatccacca
     4681 agccaaatac gtttcagcga tctggaaaaa gttagctaaa taatcaacag gagaaaacca
     4741 tgccagcgcc tctatatggt gcggacgacc cgcgccgctg ttccggcaat tccatatcgg
     4801 aggtgctgga aaatatcaag aataatctcg acgcgtttct tgctctgcca ccagaaacaa
     4861 aagaagaacg gaagtaccga cgcgatatac aactcgcaga aaaacaggaa aaagaccgaa
     4921 taaacgaaac atcaatccga ccattccgca aatccacata tacccacttc cctgaatata
     4981 tcgacccgcg cctgcgtaat taccgctcac gctatggcgc tatcagtaat gactgaggaa
     5041 ttttccatga gaggacttgc ctacaatccc ggcattcttc cggcagaaat gattattcgc
     5101 caacgcgtaa agccaatgcc atcgagagag gaattgctta aaagaaatag tttcggttct
     5161 gttaatgaca acaaatatct gaatgcgatg tggcggagtg ggaagaaatg aaacaaatgt
     5221 cactaattga gatggatggt tttctgaaag gtaaatgcat cccacgagat ttaaaggtta
     5281 acgaaacaaa cgctgaatat ctggtgcgta agttcggtga gcttgaatca aaactggaaa
     5341 cggcgttgcg ggagtgtcgt tctgctggaa tcacgattga taaccttgag gctaaatgcg
     5401 cgaagatggc tgcagaaaat acctcgctta agcaatctga gaaggaattt aatgactttt
     5461 gtcgtgagga gtttagcgaa tgggaagatg atgttactga aaccccagcc accgacactt
     5521 tcctggatga agtgcgggcg caggcgttta atgacctttg ctcggcgttc gttaaacacg
     5581 cgaatctcgc gggaatggat gatggtgagc tagtgaggct taaatatgcg acggacgccc
     5641 tgctgcattg tgcagaccaa cttcgcaagg gagtgcattc atgagcaacc tactaccatg
     5701 tccattctgt ggcggcgcag cgcacgttgc cagcgaagcg gatcaccctg aatatggctc
     5761 tggcggtcga ttctatttcg ttcgatgcgg tacgtgtcgc gctcaatctg gtagcaaata
     5821 tgcagcgcct ggaaatgact gcgcgatttt ttattcagag gttagagcag agtggaatca
     5881 gcgagcaaag gaggcaaaca gtgagcaaga ttgactatca ggcactgcgt gaaaaggcag
     5941 agaaagcaac gtgtggtgag tggtcgatcg aatatggaga tggcagcttt gatggtgatg
     6001 atgcactaat tcatcgcgag gctgctggat atattcccat ttgcagaatt gaaggagcgc
     6061 atcctgaaag cggtttcgat gaagatttcc aaatggaaca gcaggccaat gctgaattca
     6121 tcgctgcagc caatccggct accgtcttgg cgctgcttgg cgagctggaa gcagcaaaaa
     6181 agcgcatagc agaactggaa gccgaactcg taagccaaac ttacaagttg aacgagctat
     6241 cgggcaactc tccggtaact ccggatggtt ggataagctg tagtgatcga atgccggata
     6301 gcaaaacagc cgttcttgtt gccatggagt ttgacaggaa aggtgactgg cgaatgaaat
     6361 gggcgactta catcccgggg catcctgacg ctaatgatgg gtggataatt cctggtgcgt
     6421 cgtggaaacc gtcacactgg atgccgctac cagagccgcc gcaggaggcg aaatgatgga
     6481 ctccttcgca aaatatacaa ttattgactg gatggccttc cttcaggttt tgctcatctg
     6541 gttttatatg gcttacagga gtgggcagtg gattgtcagt gtagcctgta gcaagggatg
     6601 gcgttggtgg aaccgaaaga ataaaaaagc actggcattg gattcgtttt acgaagcatt
     6661 caatcttaac agccttcagc ctggttctgt cattgtagtc accactcaaa gcggcatgac
     6721 cattcagatt cataaaccaa aagaggaaaa atgatgtggc ctatatgtgt taattgcgga
     6781 cggatgtgcc tatctggatg gtgccgaaag tgcgacaaat gcacgaagaa aagacaataa
     6841 caatcctcgc actcgcgggg atttctttta tctgaactcg ctacggcggt tttttatgga
     6901 gatgataaat gcacttccga gtcacaggtg aatggaatgg agaaccattc aacagagtta
     6961 tcgaagcaga ggacatcaac gactgctata accactggat gatatgggcg cagatagcac
     7021 atgcagacgt aaccaatatt cgaattgaag aactgaaaga acacaaaaac gcctgatggc
     7081 ggttttttat tgcctgattt gcaggttcga ttccctattc ggagatagca ctcatgcaac
     7141 acgaactaca acctgattca ctggttgatt tgaaattcat catggccgat actggcttcg
     7201 gtaagacctt catctatgac cggattaagt ccggcgacct gcctaaagcc aaagttatcc
     7261 acgggcgagc aagatggtta tatcgtgatc attgtgaatt caaaaataag ctcttaagcc
     7321 gcgccaatgg gtaaaatagc gggtaaaata tttctcacat ctaaaaaaca ccattccaat
     7381 caatcccctg ctgcttcaag tagatgtctg caggggacac cagataccct tcaaacgaaa
     7441 tctaccttca ccccgtaaaa gataggtttg gcagcacact tgccttatat ctactcattt
     7501 ttactgcaac aggttgaaat ctcagcactg tcagaaagcg ctgatgacta aacagccctg
     7561 ggccgggcga tgtaaccatc acacagaatc ctgatagcga aatatggcgt gactcgatac
     7621 ttcactccgc aatgcattcc ttgatgaatt cgcaggaccg tgatacacgg gacaggtcgc
     7681 tgaatgacga caatgtcctg gaaatcagcg aaccgcgcat ctgaagtaca tttgagcgac
     7741 tgtaccagaa catgaatgag gcgtttggat taggtgatta ttagcagggc taagcacttt
     7801 agtattatta ttttccggtt gagggatagg gagatatcga caacaaccgg aaaagtttac
     7861 gtctatattg ctgaaggtac aggcgtttcc ataactattt gctcgcgttt tttactcagg
     7921 aagaaaatgc caaatagcaa catcaggcag acaatacccg aaattgcgaa gaaaactgtc
     7981 tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg aaagcagcac aatcccaagc
     8041 gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg acaggcgctt atcaaaattt
     8101 gccacgctgt atttgaagac ggatatgaca caaagtggaa cctcaatagc atgtaacagc
     8161 ttcactaatg aaataatcca ggggttaacg aacagcgcgc aggaaaggat acgcaacgcc
     8221 ataatcacaa caccgataag taatgcattt tttggcccta cccgattcac aaagaaagga
     8281 ataatcgcca tgcatagcgc ttcgagtacc acctggaatg agttgagata accatacagg
     8341 cgcgttccta catcgtgtga ttcgaataaa cctgcataaa agacaggaaa aagttgttga
     8401 tcaaaaatgt tatagaaaga ccacgtcccc acaataaata tgacgaaaac ccagaagttt
     8461 cgatccttga aaactgcgat aaaatcctct ttttttaccc ctcccgcatc cgccgctacg
     8521 cactggtgat ccttatcttt aaaacgcatg ttgatcatca taaatacagc gccaaatagc
     8581 gagaccaacc agaagttgat atggggactg atactaaaaa atatgccggc aaagaacgcg
     8641 ccaatagcat agccaaaaga tccccaggcg cgcgctgttc catattcgaa atgaaaattt
     8701 cgcgccattt tttcggtgaa gctgtcaagc aaaccgcatc ccgccagata ccccaggcca
     8761 aaaaagagcg cccccagaat tagacctaca gaaaaattgc tttgcagtaa cggttcataa
     8821 acgtaaatca taaacggtcc ggtcaagacc aggatgaaac tcatacacca gatgagcggt
     8881 ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca tcataaatag aatgctggta
     8941 aactggttga ccgaataaag tgtacctaat tccgtccctg tcaaccctag atgtcctttc
     9001 agccaaatag cgtataacga ccaccacagc gaccaggaaa taaaaaagag aaatgagtaa
     9061 ctggatgcaa aacgatagta cgcatttctg aatggaatat tcagtgccat aattacctgc
     9121 ctgtcgttaa aaaattcacg tcctatttag agataagagc gacttcgccg tttacttctc
     9181 actattccag ttcttgtcga catggcagcg ctgtcattgc ccctttcgcc gttactgcaa
     9241 gcgctccgca acgttgagcg agatcgataa ttcgtcgcat ttctctctca tctgtagata
     9301 atcccgtaga ggacagacct gtgagtaacc cggcaacgaa cgcatctccc gcccccgtgc
     9361 tatcgacaca attaacagac attccagcaa aatggtgaac ttgtcctcga taacagacca
     9421 ccacaccttc tgcaccttta gtcaccaaca gcatggcgat ctcatactct tttgccaggg
     9481 cgcatatatc ccgatcgttc tgtgtttttc cactgataag tcgccattct tcttccgaga
     9541 gcttgacgac atccgccagt tgtagcgcct gccgcaaaca caagcggagc aaatgctcgt
     9601 cttgccatag atcttcacga atattaggat cgaagctgac aaaacctccg gcatgccgga
     9661 tcgccgtcat cgcagtaaat gcgctggtac gcgaaggctc ggcagacaac gcaattgaac
     9721 agagatgtaa ccattcgcca tgtcgccagc agggcaagtc tgtcgtctct aaaaaaagat
     9781 cggcactggg gcggaccata aacgtaaatg aacgttcccc ttgatcgttc agatcgacaa
     9841 gcaccgtgga tgtccggtgc cattcatctt gcttcagata cgtgatatcg actccctcag
     9901 ttagcagcgt tctttgcatt aacgcaccaa aaggatcatc ccccacccga cctataaacc
     9961 cacttgttcc gcctaatctg gcgattccca ccgcaacgtt agctggcgcg ccgccaggac
    10021 aaggcagtag ccgcccatct gattctggca agagatctac gaccgcatcc cctaaaaccc
    10081 atactttggc tgacattttt ttcccttaaa ttcatctgag ttacgcatag tgataaacct
    10141 ctttttcgca aaatcgtcat ggatttacta aaacatgcat attcgatcac aaaacgtcat
    10201 agttaacgtt aacatttgtg atattcatcg catttatgaa agtaagggac tttattttta
    10261 taaaagttaa cgttaacaat tcaccaaatt tgcttaacca ggatgattaa aatgacgcaa
    10321 tctcgattgc atgcggcgca aaacgcccta gcaaaacttc atgagcaccg gggtaacact
    10381 ttctatcccc attttcacct cgcgcctcct gccgggtgga tgaacgatcc aaacggcctg
    10441 atctggttta acgatcgtta tcacgcgttt tatcaacatc acccgatgag cgaacactgg
    10501 gggccaatgc actggggaca tgccaccagc gacgatatga tccactggca gcatgagcct
    10561 attgcgctag cgccaggaga cgataatgac aaagacgggt gtttttcagg tagtgctgtc
    10621 gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt
    10681 aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc
    10741 gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa
    10801 gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg
    10861 gggcagatcc tgctttatcg cggcagttca ttgcgtgaat ggaccttcga tcgcgtactg
    10921 gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc
    10981 gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga
    11041 aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa
    11101 tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta
    11161 gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc
    11221 tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat
    11281 ggcaaacttc tacaacgccc ggttcacgaa gctgagtcgt tacgccagca gcatcaatct
    11341 gtctctcccc gcacaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag
    11401 attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc
    11461 actggaatgc ggctgtatat tgataaccaa tctgagcgac ttgttttgtg gcggtattac
    11521 ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc
    11581 ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg
    11641 atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac
    11701 ggagtggctg tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg
    11761 aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa
    11821 taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg
    11881 gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattacg gcgacatcct
    11941 gcgggattcg tacccccttc gccagaagaa cctgataagc cacaaaggct gcgcgatcgt
    12001 taccacatat cagaacatca aaatctggtt tgcccgattt gaagtggtca ttgagtaaac
    12061 ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca
    12121 gatctcgtcc agcatcacgc caggcctgct caaatccctg ccgacgatac cctgttgcca
    12181 acgcactttc cggtagccag aagcataacg gttgacgata gcccgccgcg agcaaatgct
    12241 gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat
    12301 ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat
    12361 gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg
    12421 ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact
    12481 cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt
    12541 cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg
    12601 aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag
    12661 gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca
    12721 ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt
    12781 agcagaaaca gacgtcactg gcgagatcca acgatattct caacgcccta tcacccaaca
    12841 cggcaataca ataaaaaata acaataattg ccggataatt gtactcaatt ctgcctctgt
    12901 tctcgccaac gagtaaccat ataggcaagt aagtaaaaca tgccaactgg catcgccaac
    12961 ctggagaaga aaatataaat atggatcatt gtgctgccgc tgtatcccga catcagcccg
    13021 gaaattattg cgattgctat cggttcaggt gcaattggct gcacgatcgt tacggactcg
    13081 cttttctggc tggtgaagca atattgcggc gctacgctca atgaaacatt taaatactat
    13141 acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt
    13201 atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc
    13261 agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac cacctggttt aatcctggca
    13321 cgacctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg
    13381 cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttcct gaaactgctg
    13441 ccactggggg gattattgaa tcagaactgg ttgccattcc agctatgcaa aaacggctgg
    13501 aaaaagaata tcagcaaccg atcagcgggc aactgttact gaaaaaagat agccatttgc
    13561 ccatttccgg ctccataaaa gcacgcggcg ggatttatga agtcctggca cacgcagaaa
    13621 aactggctct ggaagcgggg ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc
    13681 cggagtttaa acagttcttt agccaataca gcattgctgt gggctcaacc ggaaatctgg
    13741 ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagtt catatgtctg
    13801 ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat
    13861 atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga
    13921 actgtttctt tattgatgac gaaaattccc gcacgttgtt ccttgggtat tccgtcgctg
    13981 gccagcgtct taaagcgcaa tttgcccagc aaggccgtat cgtcgatgct gataaccctc
    14041 tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta
    14101 aactggcgtt tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta
    14161 tgttgttagg cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg
    14221 acaaccttac cgcagcggat ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg
    14281 caatggagcg tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc
    14341 ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc ttcggcactg gcgggtatgg
    14401 ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag
    14461 aacaactgcg taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag
    14521 aagagatgaa tcaatatctg gcaaaaggcc gttaataacg tttcaacgca gcatcgcaat
    14581 cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatccccg gtcttacccg
    14641 ctataacccc ctccttttat catcacccaa tgcctttcgc tgtaaacggc ggtttcgcaa
    14701 accaaaccaa aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg
    14761 cagaaataga aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa
    14821 gcgaaccata atatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat
    14881 cgatggttgc tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg
    14941 tcaacgacgt accaactgat cctgacaagg tacgaaaaaa attactcata ctcgaggcat
    15001 tggcaaattt attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac
    15061 aggcaacggc gaatccctga aaaaactgcg gcaaaatgat gcctgtaaaa tcaatcgttg
    15121 gcataaatgt cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctaaatgtca
    15181 ctaacaaccg catgtctatt ttgttgccat aacgtcctat caaaggtgaa attaatagtg
    15241 gcatgatgcc gatgggcgca taagcaagcc cggcccatat cgcattatac cccatcgttt
    15301 cctggagtaa ctgcggcata aggacgatcg ctccagagta aaataaatac gcgcaggtga
    15361 tactcacaat accaatggtg aaattacgag acttaaacaa actgagatca agaatcggat
    15421 tctctgaggt cgactcccaa atgactaaag agatcagaga aataactgat actactgtta
    15481 atattattat tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt
    15541 gcaagccacc aacaccgagc actaacaggg tcagtcctgg tagattcatt tttaccggtg
    15601 aagtttcagt ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca
    15661 tagggacatt gattaaaaat atccaacccc agctaaagtt atcgcaaata taaccgccca
    15721 atatcggccc acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg
    15781 ttcttttttc tggtggataa ttcctcaata ataaactctg tgacagtgga attaacggcc
    15841 ccgccattaa tccctgaacg actctaaaaa atatcagcac atcaagattg gtcgataggc
    15901 tacacattaa tgaagacagc gaaaaaaagg ttactgaaag taaaaataat cttaattcgc
    15961 ctattctttg tgccaacctg ccagtaacag ggatcgcaat ggcatttgct acaccaaacg
    16021 aggtgataac ccaggtgcct tcgtctgttg atgctcccag aaagccagat attgtcggta
    16081 ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa tgtcgctaat gacaatgcaa
    16141 tagtgacgca ccataacgtc ccaccggtta atggtgccgg agttgattta gtgattgcca
    16201 ttaaaattgt ccattatgcg aaataatgtt gctaatttct ttttctatcg gactggtatc
    16261 gataactaaa gccttactgg tataagccgg catggaggtc acggttgaag ccagctcagg
    16321 catctcggca atgtcttcgt tcttcgtatc aatagttgct gtcatcgata aaccaatacg
    16381 caagggatgt tccatgagtt cttttggatc aagagaaact tcaaccggta cacgctgaac
    16441 gattttgatc cagttccctg tcgcattttg tgcaggtaat aaggagaacg cattgccggt
    16501 tcccatattg atccctgtca cccgaccatg aaacacaaca ttttcaccat aaagatcgct
    16561 gataatattg accgattgac caatccgtac atccgtgagt tgtgtttctt taaagttggc
    16621 attaacccac atttgacgtg ccggtacgac agccattaac gattgtccgg ggctcactgt
    16681 ttcgccgacc tgaacacttc gctgggcaat atagccggta accggactct taatatccgt
    16741 acgtttaagc gccaaccagg cttctttagt tgcatccgcc gcttcaacga cttgtggctg
    16801 acggtttaat ggtgtgttca ttactaaagc tttattcgct ttataagcct ggatagcggc
    16861 attcaatgcc gctttgctac ttattaacgt atctttggta tgctccagcg tttcttttga
    16921 aataaccccc tgcttcgcta acggcactcg acggttataa tcttctaaag attgttgata
    16981 ctgaatacgt gctgaagcga cttcggcact gtattgttta tcctgtaagt atagtttatt
    17041 cgtttgccga acaatatttg ccagattatt tttagcttta ttgagtgcga tagtggcatc
    17101 agttttatcc agtgaaacta aaatgtcacc ttgtcgaacg tagttcgtat ctttatgatt
    17161 aacgacagtg acactacctg agacttgtgc agaaattgga tctgcattcc ccgtgacata
    17221 ggcgtcatct gtactaatca tgtcttctaa ttccattgac caataggcat aggcacctga
    17281 aaacgcaata aataa
//
LOCUS       DAPPNJ010000097        17220 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000097 DAPPNJ010000000
VERSION     DAPPNJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.097"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(60..791)
                     /gene="rlmB"
                     /locus_tag="RPM73_002621"
     CDS             complement(60..791)
                     /gene="rlmB"
                     /locus_tag="RPM73_002621"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDX6520967.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(971..3412)
                     /gene="rnr"
                     /locus_tag="RPM73_002622"
     CDS             complement(971..3412)
                     /gene="rnr"
                     /locus_tag="RPM73_002622"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDX6520968.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3451..3876)
                     /gene="nsrR"
                     /locus_tag="RPM73_002623"
     CDS             complement(3451..3876)
                     /gene="nsrR"
                     /locus_tag="RPM73_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDX6520969.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4081..5379)
                     /gene="purA"
                     /locus_tag="RPM73_002624"
     CDS             complement(4081..5379)
                     /gene="purA"
                     /locus_tag="RPM73_002624"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDX6520970.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5483..5680)
                     /locus_tag="RPM73_002625"
     CDS             complement(5483..5680)
                     /locus_tag="RPM73_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDX6520971.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5762..6766)
                     /gene="hflC"
                     /locus_tag="RPM73_002626"
     CDS             complement(5762..6766)
                     /gene="hflC"
                     /locus_tag="RPM73_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDX6520972.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6769..8028)
                     /gene="hflK"
                     /locus_tag="RPM73_002627"
     CDS             complement(6769..8028)
                     /gene="hflK"
                     /locus_tag="RPM73_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDX6520973.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8114..9394)
                     /gene="hflX"
                     /locus_tag="RPM73_002628"
     CDS             complement(8114..9394)
                     /gene="hflX"
                     /locus_tag="RPM73_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDX6520974.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9470..9778)
                     /gene="hfq"
                     /locus_tag="RPM73_002629"
     CDS             complement(9470..9778)
                     /gene="hfq"
                     /locus_tag="RPM73_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDX6520975.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9864..10814)
                     /gene="miaA"
                     /locus_tag="RPM73_002630"
     CDS             complement(9864..10814)
                     /gene="miaA"
                     /locus_tag="RPM73_002630"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDX6520976.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10807..12654)
                     /gene="mutL"
                     /locus_tag="RPM73_002631"
     CDS             complement(10807..12654)
                     /gene="mutL"
                     /locus_tag="RPM73_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDX6520977.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12664..14001)
                     /gene="amiB"
                     /locus_tag="RPM73_002632"
     CDS             complement(12664..14001)
                     /gene="amiB"
                     /locus_tag="RPM73_002632"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDX6520978.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14020..14481)
                     /gene="tsaE"
                     /locus_tag="RPM73_002633"
     CDS             complement(14020..14481)
                     /gene="tsaE"
                     /locus_tag="RPM73_002633"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDX6520979.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYKLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14453..16000)
                     /gene="nnr"
                     /locus_tag="RPM73_002634"
     CDS             complement(14453..16000)
                     /gene="nnr"
                     /locus_tag="RPM73_002634"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDX6520980.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15999..17138
                     /gene="queG"
                     /locus_tag="RPM73_002635"
     CDS             15999..17138
                     /gene="queG"
                     /locus_tag="RPM73_002635"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDX6520981.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17121..17174)
                     /locus_tag="RPM73_002636"
     CDS             complement(17121..17174)
                     /locus_tag="RPM73_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520982.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3891 a         4934 c         4333 g         4062 t
ORIGIN      
        1 ccagctatag acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagacgaac
       61 tagctgcgct gacgaaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcagg
      121 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
      181 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
      241 tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc
      301 aacatacgca tggtgcgcgc aaggttagtc acccgaatca gtggaacgct ttctgccgcg
      361 ccgcaggcta cttttttcgc cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc
      421 accgcgtgaa cgccagcagc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg
      481 gttacaccgt cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga
      541 tcgttttcct gatactgacg tcctggcttc acgcgggcga taatgccctg atgcacggca
      601 ccgtcgcttt tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac
      661 tcaagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
      721 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcgtggat gccgtaaatc
      781 atttcgctca ttaatgtact cgttgtttac gttttgcctg atgcactacg tttatccggc
      841 ctacatgatc tctgcaatat attgaatttg cgtgattatg taggccggat aaggcgttca
      901 cgccgcatcc ggcatgaaca aagcgcattt tgtcagcaat ctaaccctct tcttttaaag
      961 agggtattga tcactctgcc acttttttct tcgccgcacg cttcgctttg gtcgctgcgg
     1021 ctattttctg cgttttcgcc gatggctttt tcgcttttct cgcgtctttc ttcgccgctt
     1081 tcggcttcgt ttttttctca ccgcggaagg cgctgtctgg ctcaaagttt acctttttac
     1141 cgacctgacg acgcttgccg ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg
     1201 ccgttttacc gacgttgcgc ggtgcgcgtt cactggagat cagactaaag tcgattttgc
     1261 gctcgtccat attaaccgct tcgacgcgaa cttccacgcg atcgcccagg cgataagtct
     1321 ggccgctgga ttcccccatc aggcgttgcc ctacctggtc aaagcgatag tagtcattgt
     1381 ccagcgaaga gacatggacc agaccatcaa tgaacaagtc gtccagacgg acgaagaagc
     1441 caaagccagt gacgctggaa attacgcctt taaagacgtt acctacctgg tcgagcatga
     1501 agtcacactt cagccagtca gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca
     1561 tcgaacagtg ctgacccagt tgcaacatct cttccatcga ataatggtag ccgccggttt
     1621 cagtggtgtt gccctgatgc ccctgctctt tcgccagcag atacttaatg gcgcggtgca
     1681 gcgtcaggtc aggataacga cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca
     1741 ggccaaagtg accacggttt tcaggatcgt aaatcgcctg tttcatcgag cgcagcagca
     1801 tggtttgcag catttctgca tctggacgat cggcaaccga ctccagcagc tccgcgtagt
     1861 cacgcggttc cggcttatta ccgcccggca gttccagccc cagctccgcc agaacagaac
     1921 ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat acggaacagt gccggttctt
     1981 tggctttctc aacaaaacgc gccgccgaga tattcgccag aatcatgcac tcttcaatca
     2041 acttgtgtgc gtcgttacgc tgagtctgtt cgatacgttc aatacggcgt tcagcgttga
     2101 aaatgaactt cgcttcttcg ctctcaaatg agatcccgcc gcgttcttca cgggctttat
     2161 ccagcacttt atagaggtta tgcaactctt cgagatgctt aaccagtggg gcgtactgct
     2221 cgcgcagatc ctgatcgccc tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt
     2281 gagagctcat caccgcttcg tagaatttgt agccagtcag gcggcctttc gacgaaaccg
     2341 tcatctcgca caccatacac aggcggtcta cctgcgggtt gagcgagcac aggccgttag
     2401 agagcacttc cggcagcatc gggataacct gcgaagggaa gtacaccgac gtaccacggt
     2461 tacgcgcttc tctgtccagc ggcgttggcg gacgcacata gtagctgacg tcggcaatcg
     2521 cgacccataa acgccagccg ccgccgcgtt ttttctcgca gtaaaccgca tcgtcaaagt
     2581 cacgggcgtc ttcgccatca atggtgacca gcggtaaatc gcgcaaatcg acacggcccg
     2641 cttttgcttc ttccggcact tcttctttca gcccggcaac ctgttgctca acagcctgcg
     2701 gccagatgta cggaatttca tgggtacgca gagcgatatc aaccgccatg ccggtgccca
     2761 tattatcgcc aaggacttcg acgattttac ccaccgcttt ggtgcggcga gtcggacgct
     2821 gagtcagttc gactacgacc acaaagccca tccgcgcgcc catgatctga tcgggcggga
     2881 ttaagatatc gaagctcaga cggctatcgt caggaaccac aaagccgacg cccgcttcgg
     2941 taaagtagcg accaacaatc tggctggttt ttggcaccag tacgcggaca atacgcgctt
     3001 cacgacgacc tttacggtca gcgcccagcg gctgagccag cacctgatcg ccatgaatgc
     3061 aggttttcat ctgctcgctg gagagataca aatcatcttt acgcccttca acccgcagaa
     3121 agccgtagcc atcacggtgg ccaataacgg tacctttcac caggtcgagg cgttccggca
     3181 gcgcatagca ctgacgacga gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc
     3241 ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg cagttctacc gccagctcat
     3301 cacggctggc cggtttttca cgtttggtta aatgttcgag gataaattcc cgactaggga
     3361 tgggattcgc gtatttttca gcttcgcgtt cctggaaagg atcttgtgac atctcggttc
     3421 ctccgttgtc atctctgatg aagattttcg tcactccacc agcaataatt tataaagcgg
     3481 ttgattctct tcaaccaaat cggcaagcgt gtagttatcc agttccgtaa gaaaactttg
     3541 cacggcctta gaaagcgcct gtttcaaccg acaggcaggt gtaatgtggc aaaactcact
     3601 gctgcaattc accagcgata agggctccag ctcgcgcacc acatcaccaa tacgtatcgc
     3661 actcgccgat ttacccaggc gaatgccgcc attttttcca cgaacagcag tcacgtagcc
     3721 ggcacgacta agttgattga ttattttgac catatgatta cgggagacgc cgtagacgtc
     3781 agtcacttca gaaatactgg tcatccgccc ttctggcaat gacgccatgt agatcagcgc
     3841 acgtaatccg taatcagtga aactcgttaa ctgcacatca acctcaaaag ggaaatcggg
     3901 aaaaaataca tttatattga tgataaacca gccacaagct gtgtcgctaa tttatttcag
     3961 ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg atagcgtaat ggcttaaccg
     4021 acacagcaat gtatcacact gttagcccgg caggcaaaat atctgccagg cgtaccagaa
     4081 ttacgcgtcg aacggatcgc gcagaatcat ggtttcagta cgatccggac cggtagagat
     4141 gatatcgatc ggcacaccag tcagctcttc aatacgcttg atgtagttca gcgccgcctg
     4201 cggcagaccg ctacgatctt tcacgccgaa ggtggattca gaccagcccg gcatggtttc
     4261 gtaaatcggc tctacacctt tccagtcgtc agctgccagc ggagtggtcg tcacttcacg
     4321 accatccggc atacggtaag ccacacagag tttcacctct ttcaggccat ccagaacgtc
     4381 cagtttagtc aggcagaagc cagacaggga gttcagctgt accgcacgac gaacggcaac
     4441 ggtgtccagc cagccggtac gacgacgacg ccccgtagtt gcgccgaatt cgttaccctg
     4501 cttgcagagg aactcgccag tttcatcaaa cagttcggtc gggaacggac ctgcacctac
     4561 acgagtggag taagctttga ggatacccag aacgtaatca acataacgcg ggcccaggcc
     4621 ggaaccggtc gccacgccac cagcagtggt gttggaagaa gttacgtacg gataagtacc
     4681 gtggtcgata tccagcagcg taccctgcgc accttcaaac atgacgaaat cgccacgctg
     4741 acgcgcctgg tcgagcaggt cagaaacgtc aaccaccata gaagtcagga tgtcggcaac
     4801 agccatcgta tcatccagaa ctttctggta atcaaccgct tcagctttgt agtagttaac
     4861 caactggaag ttgtgatatt ccatcacttc tttcagtttt tcagcgaagg tttctttgtc
     4921 gaaaaggtcg ccaacacgca gaccgcgacg tgctacttta tcttcataag caggcccgat
     4981 accacgaccg gtggtgccga tcgctttcgc gccacgcgct ttctcacgcg cgttatccag
     5041 cgcaacgtga taatcaagga tcagcggaca tgcttcagac agcagcagac gctcacgaac
     5101 ggggatgcca cggtcttcca gttctttcat ctctttcatc agcgcggccg gagacagcac
     5161 aacaccgtta ccgatgatgc tggttacatt ctcgcggaga atacctgatg gaataagatg
     5221 gagaacggtt ttttcaccgt tgattacgag agtatggcct gcgttgtgac cgccctggta
     5281 gcgtacaaca tatttagccc gttcagtcag aagatcgacg atcttacctt taccttcgtc
     5341 accccattgg gtgcccagta cgacgacgtt gttacccatt tttcaaaatc accgtttgct
     5401 taaaaatgga ttctaccatc gctttttcag agttacagca ctttttgcac tcaaaaatga
     5461 cctcgatcag tctgtttttt gttcagccaa tcgttttcct caacatgtag tagaccacaa
     5521 cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat attatcgggc aaattggtca
     5581 tcgcagagat catcttcttc catgccttcg ggtaaagcat cggccctaaa ccttccagta
     5641 ccaaaaccag ggcaagcgcc agccagattg tcgaattcat tcattatcct tatagaaaaa
     5701 gaaaaccacc gacatccctg aggatgcggt ggctttattg acctgtaccg cagtcgttat
     5761 attaacgcgt tgcggaagtc ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca
     5821 tgaccatcac gtcctgattg ccagagaagc tgttctcata agcacgcagg ctacggatga
     5881 atgcgtagaa gtccggatct ttactgaatg catcagcaaa cagtttggct gcttcggcat
     5941 cgccttcacc acgcatgatg cggccctgac gctcagcttc tgccagcgtt ctggtcactt
     6001 catagtcggc agtcgcgcgc agtttttccg cttcttcctg accttgtgaa cggtgacgac
     6061 gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat cgcttcagac acttcggtcg
     6121 gcaggttgat ctgcttgata cgcacatcga caacttcaat acccagcgcc gccatactgt
     6181 tcgggttgat gaccggaact ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg
     6241 caatggcgtt atccgctgcc ggggtagtaa cttcatcttc tgtacccgca gaaccggagt
     6301 tcagcgcgtc acgtacttcg agggtcagac gaccacggga atcggtgacg atgtctttca
     6361 cgtccaggcg accaatttca gaacgcagac ggtcagagaa cttacgtttc aacagcactt
     6421 ccgcttgcga aatgtcgcca ccacccgttg ccaggtagta acggctgaaa tcgctgatgc
     6481 gccatttgat gtaagaatcg acgatcaggt ctttcttctc tttggtcaca aagcggtcgg
     6541 cctggttgtc catggtctga atacgtgcgt cgagcatttt caccgtttca atgaacggta
     6601 tcttgaaatg cagacccggc tcataaacca gaggtttgtt gtcatcgtca cgcagtacct
     6661 taccaaaacg cagcgtaata ccgcgctcac cttctttgac gacaaagaca gacatgtaaa
     6721 gcactaccag cacgatgatg ataatcgcga taactgactt acgcatcgtt attccccctg
     6781 acgctggtag tcgttacgct gcgcgttggc gcggcgttgg tccataatat cgccctgact
     6841 ggtggacgac gtgttgcttg ctccactggt tgtggaggaa gaggctggcg gcaaacgcag
     6901 cagattgctg gcaccgttat cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg
     6961 gtctaacggc agaaccatca ggttgccacc tttatcgtta accagcactt tgcgggtgtt
     7021 acccaacact ttttccatcg tctcgatata cagacgctcg cgagtaattt ccggcgcggc
     7081 tttatattcc ggcagaagtt tagcaaagcg cgccacttca ccctgagctt ccaggatggt
     7141 ctgggccttg tacgcacgcg cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg
     7201 ctgaacttcg ttggtatacg cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc
     7261 ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc ggacgagcag cctggaagtt
     7321 gacgtccagc agcgtgatac ccatgtcata cggacggata gtctcttcca gttcgcgctg
     7381 agtatcgcta cgaatcacgg tacgaccttc cgtcagaatg cggtccatgg tgtatttacc
     7441 gataaccccg cgcagggcgc tgtcggtagc ctgacgcaga ctgtcatccg ggctggtcac
     7501 gctatacaga tatttttccg gattggtaac gcggtactgc acgttcatct caacgcgcac
     7561 tacgttctcg tccgacgtca acatcacacc ggaagcggcc agttcacgca cggcttccac
     7621 gtttaccggt ttgacttcgt cgataaacgt cggtttccag ttcagacccg gctcaaccag
     7681 atggctgaat ttaccaaagc gtgttaccac gccgcgttcg gcttctttaa tggtatagaa
     7741 accactggcc gcccagataa tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc
     7801 aagctgcggg cgcgggcctt gcgatgaact gccaccgcca gatccggtgc ctttaccgcc
     7861 gcccagacca ccgagctttt tgctcagttt gcggaagata tcatctaaat caggtggccc
     7921 ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc
     7981 ccacgggtcg cggtcttgtc cgttattacc gggctgattc cacgccatgt ttgtgctcca
     8041 tatttgttat gcggtgatcc ctgttgtctt tcgacgccag aggatatgac tccacgcttc
     8101 agacgctacg ccgttagatc aggtaatcga tcaacgccgg ttcttgttta cagaggcgac
     8161 gccagtcaac gatcggcata cgaacttgca gacttacgct gccgtcctcc tccatccact
     8221 ctttttctat tgcctgaagc tgataaaaac gacttctcag acgcccttcc tgcggtggca
     8281 gacgcaatgt atgctgcgcc acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct
     8341 gtggtatccc cgctccggtc tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt
     8401 cccgatcaat acgcggttcg aaatcttcca gcatatcgat cttgttcatc accagcaggg
     8461 ttgggatctc gtgagcgtcg atctcttcaa gaaccgtatt caccgcttcg atgttttctt
     8521 gtacacgtac atccgccgca tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt
     8581 gtaacgtggc tttaaatgct gcaaccagat cgtgcggcag gtggcgaata aaccctacgg
     8641 tatctgccaa cacggtttca ccgacatctg caacgtcaat acgccgcaac gtcgggtcga
     8701 gggtggcaaa caactggtct gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg
     8761 tagatttacc ggcgttggta tatcccacca gcgaaacagt aggaacgtcg gctttgatac
     8821 gcgattgccg cccctgctca cgctgctttt caactctttc caggcgcgac tgtatctgca
     8881 cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca
     8941 aacctatccc gcctttctgt ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca
     9001 gatggcgcag ctgcgccagc tcaacctgca acttaccctc atgggtacgc gcacgttggg
     9061 cgaaaatatc taaaataagg ccggtgcggt cgataacacg acactcgcac aaacgctcca
     9121 ggttacgctc ttgcgccggg ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg
     9181 ctttgacagc ttccgcaatt tcaactgctt taccttcacc tacaaaatac tttgggtgcg
     9241 gcgctttacg gctaccggta atcacctgca atgcttcgac accggcggaa gagaccagag
     9301 attcaaactc ctggaggtct tccatatctt tgtcttgcgt aaaatagatg tgtaccagta
     9361 ccgcctgctc accagcatca taacggtcaa acaagcgtat aaccctctaa atagatcagc
     9421 ggggaacgca ggatcgctgg ctccccgtgt aaaaaaacag cccgaaacct tattcggttt
     9481 cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct accatgatgg tagttactgc
     9541 tggtaccgcc accggcgttg ttactgtgat gagaaaccgg acgagacggg acaacagtag
     9601 aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt caacaggatc acgaactgat
     9661 caaaagactc gatttgccct tgcagcttaa taccattcac caaataaata gaaactggaa
     9721 cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa agattgcccc ttagccattc
     9781 tctcttttcc ttatatgctt atttgtactt tgaacctttc gattctgaaa aaattgcgca
     9841 cgatacgtct caattgtaca cattcagcct gcgatagcac caacaacctg taatacttcg
     9901 tcacgcgcct gttctggttt ttcactgtca agccagtgaa ccccttccca accacgcagc
     9961 caggttatct gccgcttcgc caactgtctc gtggcgcaaa cacctcgata aaccatttca
    10021 tcgtatgaga tttcgccttc aaggtaagac cacatctggc gataacccac gcaacgaatg
    10081 gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa
    10141 cctgaagcca acatctgatg aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc
    10201 gccggggcga tggcgaactg atgcacctga tacggtagag cgtctcctga cgtttgcgtc
    10261 agttccgtta aagttttacc cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt
    10321 ggatcatttg gatgaatcct tgctgccgca accggatcta cctcctgaag ttgacgatgc
    10381 aatgactccc aaccttgctc tgccgcctgt tgctcaattc tggcccgcac ttccgggtct
    10441 gccgacggta gcggcgacaa cccttccagc aatgccttga aatacaacat cgtaccgccc
    10501 actaacagtg ggatccgccc cgccgcggtg atatcggcca tttccgccag cgcatcgcgg
    10561 cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc
    10621 gcggcgagta actcttcagc gttcggcttc gccgtcccga tatccatccc tttgtaaata
    10681 agggcagaat caacgcttat caactctact ggtaaaattt tacgcagctc aatggctaac
    10741 gccgttttac cggaggccgt cggccccatc aaaaaaatcg ccttaggcag gctcgcctta
    10801 ctgatatcac tcatctttca gggcttttat cgccggatgt aaatcaacag attgtaacag
    10861 accacccggc ggcgttttca caagttgcgg acataaccgt tccacgtccg ccagcagggt
    10921 tatggcctgt gccattgacc actgcgcatg ttcgctcatc agatttcgtg caatccactg
    10981 cgcaatattg ccaggttcga atacggactg cttcgccagg tagcctatca gttcaggaat
    11041 caagatttgt aaattttgtt ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg
    11101 tgcatctgac tggaaatcaa tacccaattc cgccagggca gactgcgctt tttctaatgc
    11161 cgatttttct tcggcagaaa cttttagccg caacggaatc agcagcggct gggcgcaaac
    11221 gggcgcttca cccggcgtca attgtgcctg acgtagccaa cgttctgcca ctggcaagga
    11281 taaaagtgaa atgttgccat cgcgctccag caacgcacag tcggaatgga cgatagtcag
    11341 tacccgacca aaactctgac tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt
    11401 tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga tacacttcac cttgctgttt
    11461 ctggtagcct ggctgcgcat tcggccaggg ggcagccgga cgactgcctg atgctggcgc
    11521 aggagtgtag cgcggagcta ccggctcacg agctgccggt tctgcaaagt gattgcgccc
    11581 cgccgccacg cggttttccg gaatggaacg cggtgcaggt tggggttcat cgtccagcgg
    11641 tagcggcgtt tccagttgct gttgtagcac gctcagcacg ccctgataga taaaatcatg
    11701 caccagacgc gactgatgga aacgcacttc gtgtttggcg gggtgcacgt tgacgtccac
    11761 ctgatgcgga tcgatctcca gatacagcac aaatgccggt tgctgatcgg cccccagttt
    11821 gtcttcgcag gcctggcgga tcgcgtgatt gatcaggcga tcgcgcatca tgcgaccgtt
    11881 cacgtagcaa tactgaattt ctgccagtgc gggcgtggtg tgatttggat cggccaccca
    11941 gccgcgtagc gtgagatcgc cgtgttgcca ttcaatcgcc agcgcttgtt caagaaaagc
    12001 ggtgccgcaa atcgcgccta agcgccgttc tttttgcccg ccttccggca ctgcgcggta
    12061 ctgacgcaca attttaccgt tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc
    12121 aatgcggcgg atgatctcat caatgtggtt aaattcggtt ttctcggtgc gcaggaattt
    12181 gcgccgcgcc ggggtgttgt agaacagatc cagcacctcc agcgtcgtcc ccaccggatg
    12241 cgccgccggt tttaccgtca cgttcatatc gcgcccttcg gcataggcct gccaggcttc
    12301 ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa accgaactga tactcgccag
    12361 cgcctcaccg cgaaagccca ggctgataat ggcttcgaga tcgtccagag aggcgatttt
    12421 actggtggca tgacgagcca gcgccagcgc cagctcatct tttttgatac cgcagccgtt
    12481 atcacgaatg cggataagtt tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc
    12541 acctgcatcg aggctgtttt ccaccagttc tttgactacc gacgcaggtc gctcgaccac
    12601 ctcacctgcg gcaatctggt tcgccagttg tggcggtaag acctgaattg gcatcaatcg
    12661 tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc
    12721 cccctgcggc gcagattgca tcggatgcgc aaggaaataa ttgcgcaggc ctttgtaaat
    12781 ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt
    12841 gctgataaaa ccggtttcga ccagtactga cgggatatcc ggcgaacgca gaacgccgag
    12901 actcgcgtgt tctggtcggc gtttatgtat ttcgccaatg cgttgcaact gactgatcat
    12961 actggtcgct acatcatacc ctacccgttg ggaatgaccg aactgtaaat ccagcaccgc
    13021 ctggcttaaa taggggtcag actgactgtt cgccagcaca tcacccgccc cacccagcag
    13081 ctccgactgt ttctcgtgct gctccagcca gctggccatt tcgctgttgg cgcgacggtt
    13141 agagagcacc catacggaag cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat
    13201 cgacacgagg aaattggcgt tttgcttacg tgccacatca ctgcgcccca tcaccgagat
    13261 aaagtaatcc ccgtcacggg ttaaaacgcc cttaaacatc ggatcgtcat tgagcaaagt
    13321 acgcaattta cgcgcgatgg cgatggtgac atttttctcc cgcgtaccac cggggccgat
    13381 agcgccaggg tcctgaccgc cgtgtccggc atcaatagcg atgataattt tatcgccagt
    13441 gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta ctgctgataa cacccgtagt
    13501 gcggttactt tccgttttaa acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac
    13561 cgcaggcgtt tcaacgcgtt tcgcaaccac gggcggcgga ggaggaggtg gcggcgcatc
    13621 ggcgttaatc gtaaagacga cagtgtaatt gctgccattc tgccgcttca ccgcttcggt
    13681 tttaccgttt tcggtaagat cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc
    13741 agagcgaatc gccttcacca gattattgcc gctgaacaac aacggcagtc cctgaatcac
    13801 gcccgtttgt ttgatatcga gcgccacggt gcgtttgctt tgatggctaa acgcataatc
    13861 aggatcgcca ataaaactca acgttatccg cgcctgttga ttaccgttag aaacctgaat
    13921 atcagagagc gtcgcggcac ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca
    13981 atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag
    14041 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    14101 atcaatgtgt atttcgacat ccgggtcagg aagaacacca gtaccttgtt gtggccactc
    14161 caccaggcag atggcatcgt tggcaaaata atcgcgaatc cccataaact ccagctcctc
    14221 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcttataggg
    14281 ttcgaccagc gtataagtgg ggcttttgac attaccctga tgacccagag cttgtaaaaa
    14341 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    14401 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    14461 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    14521 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    14581 cagctgcagc accgtgcgcg acacagcccg cacaggcggc atcatacggc gacagttttt
    14641 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc
    14701 ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg
    14761 cacctttcag caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc
    14821 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    14881 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    14941 catcccacaa catcggtttg cgaaaattct caactttttg cagtgctttt ttcccccact
    15001 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    15061 gagagtccat cgtcagttca tgcaccatca attccggtcg tgcagtcagc agcggcgcaa
    15121 tgttttcact gcgggtcagt actcggacta aaccagcacc agcacgtagc gccgcttccc
    15181 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataattacc agccgcccgt
    15241 gatcgccttt atgcgaagtc gggcgacgcg gttttagcca gtgagaaagt tgttctgccg
    15301 aaaaccgctg aattttcgtc tcctgacctg ccagccaact atccagcccc agtgagtcaa
    15361 aatgcagttg tccggtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    15421 taaaagtgat ggtgtgatcg gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca
    15481 gcaggccgga agggatatca accgccgcaa tcggcgcagg atgggaatta gcgtggtcga
    15541 ttaactggct aatggattcg cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa
    15601 caatcagatc taccgattcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt
    15661 ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtcg ctctcctggg
    15721 ccagcagcgt gacctcaatg ccgaccgctg tggccagccg cgcgaccacg tagccatcgc
    15781 cgccgttatt accatgaccg cacagcacca gccaatggcg ggcgtcagga tacgccgaac
    15841 gacacacctg gaatgcggct tcgccagcgc gaagcatcag ctcatagagt gtaagcccca
    15901 gcgcatctgc cgcctcacgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg
    15961 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    16021 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    16081 ttaccgatac cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat
    16141 accacggcga aatggactgg atggcacgtc acggtatgct gcgcgctcgc ccccatgaat
    16201 tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    16261 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    16321 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    16381 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt
    16441 tagctgaaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    16501 ccggttcttt cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac
    16561 cagtcgagga aggatgcggt aaatgcgtgg cctgtatgac gatttgcccg accggtgcca
    16621 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaacttg
    16681 aaggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg
    16741 actgccagct tatctgcccg tggaatcgct attcacaact caccacagaa gaggatttca
    16801 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    16861 agtttttaaa agtcacggaa ggatcggcga ttcgtcgtat tggtcacctg cgttggctgc
    16921 gtaatatcgc cgtagcatta ggcaatgccc cttgggatga aacgattttg acagcgctgg
    16981 aaagtcgtaa aggtgagcac ccacttctcg atgagcacat agcgtgggcg attgcgcagc
    17041 aaatagagag acgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg
    17101 ttcgggtgat tgaaaaaggg ttaccgcgtg acgcctgatt catccacagc ttgtgaataa
    17161 aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa
//
LOCUS       DAPPNJ010000098        16900 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000098 DAPPNJ010000000
VERSION     DAPPNJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.098"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1125
                     /gene="ompN"
                     /locus_tag="RPM73_002637"
     CDS             <1..1125
                     /gene="ompN"
                     /locus_tag="RPM73_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDX6520983.1"
                     /translation="KVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDN
                     SAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFA
                     DYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLV
                     NGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSS
                     DRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQ
                     NFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFN
                     KNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1266..1700
                     /gene="uspF"
                     /locus_tag="RPM73_002638"
     CDS             1266..1700
                     /gene="uspF"
                     /locus_tag="RPM73_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDX6520984.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1876..2811
                     /gene="ttcA"
                     /locus_tag="RPM73_002639"
     CDS             1876..2811
                     /gene="ttcA"
                     /locus_tag="RPM73_002639"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDX6520985.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2940..4313)
                     /gene="dbpA"
                     /locus_tag="RPM73_002640"
     CDS             complement(2940..4313)
                     /gene="dbpA"
                     /locus_tag="RPM73_002640"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDX6520986.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4791..5774)
                     /gene="zntB"
                     /locus_tag="RPM73_002641"
     CDS             complement(4791..5774)
                     /gene="zntB"
                     /locus_tag="RPM73_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDX6520987.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6029..7261
                     /gene="dgcM"
                     /locus_tag="RPM73_002642"
     CDS             6029..7261
                     /gene="dgcM"
                     /locus_tag="RPM73_002642"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDX6520988.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7282..7845)
                     /gene="smrA"
                     /locus_tag="RPM73_002643"
     CDS             complement(7282..7845)
                     /gene="smrA"
                     /locus_tag="RPM73_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDX6520989.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8175..9083)
                     /locus_tag="RPM73_002644"
     CDS             complement(8175..9083)
                     /locus_tag="RPM73_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6520990.1"
                     /translation="MTFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9259..10569
                     /gene="abgA"
                     /locus_tag="RPM73_002645"
     CDS             9259..10569
                     /gene="abgA"
                     /locus_tag="RPM73_002645"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDX6520991.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10569..12014
                     /gene="abgB"
                     /locus_tag="RPM73_002646"
     CDS             10569..12014
                     /gene="abgB"
                     /locus_tag="RPM73_002646"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDX6520992.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12051..13577
                     /gene="abgT"
                     /locus_tag="RPM73_002647"
     CDS             12051..13577
                     /gene="abgT"
                     /locus_tag="RPM73_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDX6520993.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13588..14103
                     /gene="ogt"
                     /locus_tag="RPM73_002648"
     CDS             13588..14103
                     /gene="ogt"
                     /locus_tag="RPM73_002648"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDX6520994.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14298..15050
                     /gene="fnr"
                     /locus_tag="RPM73_002649"
     CDS             14298..15050
                     /gene="fnr"
                     /locus_tag="RPM73_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDX6520995.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15202..16152
                     /gene="uspE"
                     /locus_tag="RPM73_002650"
     CDS             15202..16152
                     /gene="uspE"
                     /locus_tag="RPM73_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDX6520996.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16202..16459)
                     /locus_tag="RPM73_002651"
     CDS             complement(16202..16459)
                     /locus_tag="RPM73_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDX6520997.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16703..>16900
                     /locus_tag="RPM73_002652"
     CDS             16703..>16900
                     /locus_tag="RPM73_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HDX6520998.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIE"
BASE COUNT         4297 a         4208 c         4120 g         4275 t
ORIGIN      
        1 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
       61 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
      121 ttttctgata attcggcgaa agatggcgac cagagctatg cgcgtctggg ttttaaaggc
      181 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
      241 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
      301 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
      361 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
      421 atgactggtc gagccaatgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
      481 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
      541 gaaggcacca acaacggacg tgatgttcgc catgaaaacg gtgacggctg gggtctttcc
      601 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc ttctgaccgc
      661 accaatgatc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
      721 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
      781 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
      841 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
      901 ggccgtgacc tgcacgctgc cggtggtgca gacaacccgg caggtgttga tgataaagat
      961 ctggttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
     1021 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caatggcatc
     1081 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
     1141 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
     1201 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
     1261 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
     1321 gcgtgattag ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga
     1381 cggtaatacc ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac
     1441 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
     1501 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
     1561 ttctggaaat ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
     1621 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
     1681 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
     1741 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat
     1801 cagatttctt atgctgggcg ttccggcatg acgcatactc ttctgatgcc atataacgaa
     1861 ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg
     1921 aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg
     1981 attgaagaag gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg
     2041 ctggagattc tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt
     2101 aacctcgatc aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag
     2161 ctgggcgttg agtacaagat tgttgaagag aatacttacg gtatcgtgaa agagaagatt
     2221 ccagagggca aaaccacttg ctcactgtgt tctcgccttc gtcgcggtat cctttatcgt
     2281 accgcaacgg aactgggggc gacgaagatc gcgttgggtc accatcgtga cgatatcctg
     2341 caaacgttgt tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg
     2401 atgagcgatg atggcaaaca tatcgttatt cgtccgctgg cctactgccg cgagaaagat
     2461 attcagcgat ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca
     2521 cagcctaacc tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat
     2581 ccagggcgta tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc
     2641 gataccaacc tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt
     2701 gatctggcgt ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa
     2761 gatgaaaatc agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag
     2821 cgcccgatgc gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg
     2881 tcgcatccgg caatcccgca taagatgttt actcttgcac ccggcaattc aacatttcat
     2941 tattttaata accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc
     3001 catgctttat gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg
     3061 attttgccaa tatctacgcc atcaagcccg atgtctcctg tcagcgcacc taatacatca
     3121 cccgggcgca ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc
     3181 agcggcaaaa tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg
     3241 tcagaaatga tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg
     3301 ctatttcctg cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc
     3361 agctcaaagt tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca
     3421 gtcgcaacca gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgatcg
     3481 cgttgctcca gatcgccgtg taacgacaat gcactttgcc ctacttcatt cagcgcgtcg
     3541 cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga tggctgatgc
     3601 aaacttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc ataaaattgt
     3661 tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg atcgcgttgc
     3721 actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag aagcgtctgt
     3781 cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc catatccagc
     3841 atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac cgtgcctttt
     3901 tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc atgttgcaac
     3961 gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt ggtatttggc
     4021 agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc acgcgtagga
     4081 cacagcacta aagcctgggt ttgaaatagc gacgcatcaa tttgctgtaa caagccgagg
     4141 ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc ttttccggca
     4201 aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc caactcatta
     4261 aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt cacaatctat
     4321 tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta cgctcacgat
     4381 gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc gggcgaggga
     4441 tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga atttgcaaat
     4501 aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg gtagacgctg
     4561 ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga atcaattgtg
     4621 ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc atcgcttgac
     4681 gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta ttgacttcgc
     4741 tcaaactttg cggcgttttt gtatacagac agccggaaaa attgcttttg ttacaaccat
     4801 ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag aatacaaaaa
     4861 attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac gccaaataac
     4921 cctgtcagaa aggtactggg taaaaagacc attgccatca atgacattgt ataggtacga
     4981 cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacacc agtccgtgct
     5041 atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc ctgcatccga
     5101 cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc atagacatca
     5161 cgttgcggtg ccatatagcg acgcatcacg attaattgtt tgcgcagcag agccaggaat
     5221 ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat tttatcgtgc
     5281 agctgctcga taaattcgct ggaatgatcg gtcaacgcat cgcacacatc caccagccac
     5341 cctccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc gtccagcgcc
     5401 agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac acgcatggcg
     5461 accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt aataagcgtg
     5521 ccttcaccga gacggctgac tcggggccgt gtgctctcgc ccgccagcgc atcacgtacg
     5581 ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg tacataatta
     5641 aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag cggtttaacg
     5701 ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac gtccgatccc
     5761 ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta gccttctggc
     5821 cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa ttctgtctaa
     5881 attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg ttaaatagcg
     5941 tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc ggatagcaac
     6001 aaaaattgat aaaaataatg ggatctcaat gattacgcac aacttcaata ccctggactt
     6061 actcaccagt cctgtctgga tcgtttcgcc ctttgaggaa cagttaattt atgccaatag
     6121 cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg gaccctattc
     6181 cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc aacacgatat
     6241 tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct gtcggcttgt
     6301 tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg aagcgccggc
     6361 aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac aaggttttta
     6421 tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccct cacgagatgg
     6481 ccaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc atgaaacgat
     6541 gtgccagaaa catacctggg aaattaatat gctcgggcgt cgcgtcatgc ctatcatgca
     6601 tgaaatttcg catttacccg gcggtcataa acctttgaat tttgttcata aactggcgga
     6661 tggatctact cgtcatgtgc agacctatgc cgggccgatt gaaatttatg gcgacaagct
     6721 catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc agctggaaca
     6781 tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt atcacattac
     6841 cgagccaggc caaatgcagc acctcgccat cgcccaggat tacagcttgt tgctcatcga
     6901 caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg atgaggtgtt
     6961 atgcgccctc gcccgcaccc tcgaaagttg cgctcgcaaa ggcgatttgg tgtttcgttg
     7021 gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg cgctttcgct
     7081 ggctgaaact atccgtgtaa gcgtggcaaa agtgagtatt tcgggcttac cacgttttac
     7141 cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac tgtttaaacg
     7201 cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc tggcggcata
     7261 agccgcggat gcgtctcgag atcaacggct gcgcttagcg tggcgctccc agttttcttg
     7321 cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc catgatgcgg
     7381 tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg ccacatagct
     7441 gcggacaata ttggcatgcg atttatcatc ccgtcctttg ccatgaataa tcagcacgtt
     7501 acgcaaacca tcagccatgg cttgttgaat gaaactgaac atcattttgc ggcactcttc
     7561 caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac tgcgcagctt
     7621 atccagcacc ccatgttgca acccttcccg ccgaaactcc agcggctgac ttagtgggat
     7681 gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc agcgtgtcga tacgctgcgg
     7741 cgcacgttgg ttacgcgttg gatgccagtg gacatcggta gcacgtttca gcggctggac
     7801 atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgttac atcctcccgc
     7861 aattaagagc gcgatatgat aaccagaccg ggtcggtcca acaacgtatt acccaaaatt
     7921 ccagtaataa gttccaaata ttgccgatat tttaagcaaa atgcttatgc atgattattc
     7981 attcacgata ttaataatgt aacttatatt ttcgtgaaat ctgtcactga agaaaattgg
     8041 caactaaagg ttaaaaccgt tataacacag tcaccggcgc agaggagaca atgccggact
     8101 taagacgcgg atgcactgct gtgtgtactg tagagtctgg cggatgtcga cagactctat
     8161 ttttttatgc agttttaact ttgcagatag ccgcattctc gccggaattg cgtgattaat
     8221 gacgcggtca gtggtgtctg gcgactatca cgccgctgaa tcaaataata ggccgctttc
     8281 ggtaaaattt cgctaaccgg caacatcacc agcccctgtc cgtgcaaggg atcgcagccc
     8341 atttcttcag gcagtatgct gagaaaatcg ctttttgcca ccagactgat acaggctgag
     8401 aacgtctcgc agactacacc gacctgtggc gtttgcgcct gatcgtcaag caattcactc
     8461 aactgtttgt agtaactgcc gtgtggcgtc ggcattgtcc agctgtaatc cagtaactgt
     8521 ttgatcgaac gggcaccaat ggcggggtgt cccgggcggc aaaagatcgc gaattgcttt
     8581 tccagtaatt tctcaaaagt aaattcgtgg tcgtacggtc cctgataata ggtattgatg
     8641 gtgaaatcca attctccctg acgcaattca ttaatcatcg acaccagttg cccttccata
     8701 atgcgtactt ttacctgcgg atgctgctga tgaaaacgag atatgacagc tggcatcaga
     8761 ctgcgggaaa tactggcccc catgccgata ttaatctgcc ctgccagttg cccttgtcgt
     8821 tggcgaattt cctcttgcgc tgcgcgcagc tcttcaagaa taagactggc gtgctgataa
     8881 aaactttcac cggcatcagt taacgtcacg cctttactac ggcgaaaaaa gagttgcgcc
     8941 gctaatcctt cttccagctc ctgaatagat ttactcagtg ccggttgcga catattcaac
     9001 attcggctcg ctccgcgaat gctgccctga cgagccactt caacaaaagc acgaatttga
     9061 tgaattttta cctgaaaagt catgacgcca ccgataaccg ttatttatca gaccaaagaa
     9121 actggcatct actttaatgc agatgattgt gtcagggtaa tttatgaacg gttaaaactg
     9181 tgaaaaatca gttagtgata agtaaaaact atcgctacgt gaaccgggtc acacttttta
     9241 ctgatgacgg gaaaggttat ggagtctttg aatcaatttg ttaattcgct tgccccaaaa
     9301 ttatcgcact ggcgacgtga ttttcatcac tatgcagagt ctggctgggt ggaattccgc
     9361 actgccaccc ttgttgcgga agaattgcag cagctcggct attcactggc gctgggccgc
     9421 gaagtagtta atgaaagtag ccggatggga ctacctgatg aactcactct acaacgcgaa
     9481 ttcgagcgcg ctcgtcaaca gggtgcgcta gcacaatgga ttgcggtttt tgaaggtggt
     9541 ttcactggta tcgtcgccac cctggatacc ggtcgccccg gtccggtgat ggctttccgt
     9601 gtcgatatgg acgcgctgga tctcagtgaa gagcaggatg tcagccatcg cccctaccgc
     9661 gacggttttg cgtcatgtaa cgccggaatg atgcatgcct gtggtcatga tggacatacc
     9721 gccattgggc ttgggctggc gcataccctt aaacaattcg agtccggact acatggcgtc
     9781 atcaaactga tttttcagcc tgcagaggaa ggtacgcgtg gcgcgcgggc gatggtcgat
     9841 gcaggtgtcg tagatgatgt tgattatttt actgccgtgc acattggcac tggcgtacct
     9901 gcgggcaccg tggtgtgcgg cagtgataat tttatggcaa ccaccaaatt tgacgcgcac
     9961 ttcaccggga ccgccgctca cgcaggcgca aaaccagaag acggtcacaa tgccttgttg
    10021 gcggcagcac aagcaactct tgcactgcat gcaatcgccc cgcacagcga aggagcttcc
    10081 agagtaaacg tgggcgttat gcaggcagga agcggccgta acgttgttcc tgcctcggcg
    10141 ttgctgaaag tggaaacacg cggggccagc gacgtcatta atcaatatgt ttttgaccgt
    10201 gcacaacaag cgattcaggg cgcagcaacc atgtatggtg tcggcgttga aactcgtctg
    10261 atgggtgcag ctaccgccag ttctccttcg ccgcaatggg tcgcatggtt gcaaagtcag
    10321 gcggctcagg tcgcgggggt caatcaggcc attgaacgtg ttgaagcgcc tgcgggttcc
    10381 gaagatgcca cattaatgat ggcccgcgtg cagcaacatc aagggcaagc ctcctacgtg
    10441 gtgtttggca cacagctggc ggcaggtcat cacaacgaaa aattcgattt tgacgagcag
    10501 gttctcgcta ttgccgtcga aacgctggcg cgcaccgcgc tcaattttcc ctggacgcga
    10561 ggtatctgat gcaggaaatc tatcgtttta tcgacgatgc gattgaagcc gatcgccaac
    10621 gttataccga tattgccgat caaatctggg atcatccaga aacacgtttt gaagagttct
    10681 ggtcagcgga gcatctggct tcggcgctgg aatctgcaag cttcaccgtt acccgcaacg
    10741 taggcaatat cccaaatgca tttattgctt cgtttggtca aggcaaaccg gttatcgccc
    10801 tgctgggaga atatgacgcc ctggcaggtt taagtcagca agctggttgc gcgcaaccta
    10861 catccgcgac gcccggtgaa aatggtcacg gttgcggaca caatttgctg ggaaccgccg
    10921 cctttgccgc tgcaatagcc gtcaagaaat ggctggaaca atatgggcaa ggcggcacag
    10981 tgcgctttta tggttgtcct ggcgaagaag gcggctcggg taaaacgttc atggtccgcg
    11041 agggggtatt tgatgatgtg gatgcggcac tcacctggca cccggaagcc tttgccggca
    11101 tgttcaatac ccgcacgctg gcaaacattc aggcatcatg gtgctttaaa gggatcgcag
    11161 cacatgccgc gaattcccct catttgggac gcagcgccct tgatgccgta acgttgatga
    11221 ccactggcac caacttcctc aacgaacata ttattgaaaa agcgcgcgta cactatgcca
    11281 tcacaaatag cggcgggatc tcgcccaacg tggtccaggc gcaggcagaa gtgctttatc
    11341 ttatccgcgc ccccgaaatg accgacgtgc agcatattta tgatcgggtc gccaaaatcg
    11401 ccgaaggtgc ggcattgatg accgaaacca cggttgaatg ccgcttcgac aaagcctgtt
    11461 ccagttatct cccgaatcgc accttagaaa atgccatgta ccatgcccta tcccattttg
    11521 gtaccccgga atggaactcc gaagaactgg cttttgcgaa acaaattcag gctacgctca
    11581 cccccaacga tcggcaaaac agtctgaata atatcgccgc aaccggtggc gaaaacggca
    11641 aggtttttgc actacgtcat cgtgaaacgg tactggcgaa tgaggtcgct ccatatgccg
    11701 ccaccgataa cgtgcttgcg gcatcgactg atgtcggcga cgtcagttgg aaactgcctg
    11761 ttgcccagtg tttcagcccc tgttttgccg tcggtacacc gctacatacg tggcaactgg
    11821 ttagccaggg gcgaacatct attgctcata aaggaatgct gctggcggcg aaaactatgg
    11881 cagcaaccac agtcaatctc ttcattgatt cagggctatt gcaagaatgc caacaagagc
    11941 atcagcaagt tacggacacg caaccgtatc actgccctat cccgaaaaac gtgacaccgt
    12001 cacctttaaa ataacaacaa caacgcaaac acaacaaccg aggaatgccc atgagtatgt
    12061 catccatacc gtcgtcctcc caatccggga agctctatgg ctgggtcgaa agaattggta
    12121 acaaggttcc ccatcctttt ttgctcttta tctatttgat aatcgtactc atggtgacga
    12181 cggcaatttt gtcagccttt ggcgtcagtg cgaaaaaccc gaccgatggt acgccggtgg
    12241 tggtgaaaaa cctgctcagt gtggaaggat tacactggtt tttacccaat gtgattaaaa
    12301 actttagcgg ttttgctcca cttggagcga tcctggcgct ggttttaggt gccggcctag
    12361 cggagcgcgt cggcttactg ccagcgctaa tggttaaaat ggcatcgcat gttaatgccc
    12421 gctacgccag ttatatggtg ctgtttattg cttttttcag ccacatttct tccgatgcgg
    12481 cgttagtgat catgccaccg atgggtgcgc tgatttttct ggcggtgggc aggcatccag
    12541 ttgcaggttt actggccgcc attgcaggcg taggttgcgg ctttacggct aatttactga
    12601 ttgtcacaac cgacgtgttg ctgtcgggga tcagcacgga agcggcggct gcgttcaatc
    12661 cgcaaatgca cgtcagtgta attgataact ggtattttat ggccagctcc gtagtcgtac
    12721 tgacgattgt tggcggcctg ataaccgaca aaatcatcga gccacgatta ggtcaatggc
    12781 agggaaacag cgatgagaaa ctgcagacat tgaccgaaag tcagcgtttt ggtttacgca
    12841 tagcaggtgt cgtatcgcta ctttttattg ctgcgatcgc gctgatggtg atcccggaaa
    12901 acgggatatt gcgcgatccg attaatcaca ccgtgatgcc atcacccttt attaaaggta
    12961 tcgtgccact gatcattctt tttttctttg ttgtctcgct ggcttatggc atcgctaccc
    13021 gcacaattcg acgtcaggcg gatttaccgc atttaatgat tgaaccgatg aaagagatgg
    13081 cgggatttat cgtgatggtt tttcccctcg cccaatttgt cgccatgttt aactggagca
    13141 acatggggaa attcatcgcc gtggggctga ccgatatact tgaaagttca gggcttagcg
    13201 gcatcccggc gtttgtcggt ctggcgttgc tttcctcttt cttatgcatg tttattgcca
    13261 gcggttccgc aatctggtcg attctggccc ccattttcgt accaatgttt atgctacttg
    13321 gctttcaccc ggcatttgcg caaatcctct ttcgtattgc cgactcatcc gtattgcctt
    13381 tagcgccggt atctcctttt gttccactgt ttcttggatt cctgcaacgc tacaaaccag
    13441 acgcaaaact gggtacttac tattcgttag tcttgcccta tccgcttatc tttttggtgg
    13501 tatggctgct gatgttgctg gcgtggtatc ttgttggtct gccgataggt ccggggattt
    13561 acccacgttt gtcttaagag agaacggatg ctgagattac ttgaagaaaa aattgccacg
    13621 ccactgggtc cactgtgggt gatttgcgat gagcaatttc gcctgcgggc ggttgaatgg
    13681 gaagagtaca gcgaacgcat ggtgcagctg ctggacatcc attatcgcaa agaaggctat
    13741 gagcgcattt ctgccaccaa cccaggcggt ttaagcgaca agcttcgtga ttattttgcc
    13801 ggtaatctta gcattattga tacgcttccc actgccacag gggggacgcc atttcagcgc
    13861 gaagtctgga aaactctacg cactatcccc tgcgggcagg taatgcatta cggccaactg
    13921 gctgaacaat tgggccgccc tggcgcggcg cgtgccgttg gtgcggcaaa cggatcgaat
    13981 cccatcagca tcgtcgtacc ttgccatcgg gttattggcc gaaacggcac catgaccgga
    14041 tatgcaggcg gagttcagcg aaaagagtgg ttattgcgcc acgaaggtta tcttttgctg
    14101 taaacattaa acaatttgtg ccagcttgtt cacactttta tgtaaagtta cccttaacaa
    14161 cttaagggtt ttcaaataga tagacatata tttacatctg atatcggaat tctctgctgt
    14221 taaggtttgc ttagacttac ttgctcccta aaaagatgtt aaaattgaca aatatcaatt
    14281 acggcttgag cagacctatg atcccggaaa agcgaattat acggcgcatt cagtctggcg
    14341 gttgtgctat ccattgccag gattgcagca tcagccagct ttgcatcccg ttcacactca
    14401 acgaacatga gcttgatcag cttgataata tcattgagcg gaagaagcct attcagaaag
    14461 gccagacgct gtttaaggct ggtgatgaac ttaaatcgct ttatgccatc cgctccggta
    14521 cgattaaaag ttataccatc actgagcaag gcgacgagca aatcactggt ttccatttag
    14581 ctggcgacct ggtgggattt gacgccatcg gcagcggcca tcacccgagc ttcgctcagg
    14641 cgctggaaac ctcgatggta tgtgaaatcc cgttcgaaac gctggacgat ttgtccggta
    14701 aaatgccgaa tctgcgtcag cagatgatgc gtctgatgag cggtgaaatc aaaggcgatc
    14761 aggacatgat cctgctgttg tcgaagaaaa atgccgagga acgtctggct gcattcatct
    14821 acaacctgtc ccgtcgtttt gcccaacgcg gcttctcccc tcgtgaattc cgcctgacga
    14881 tgactcgtgg cgatatcggt aactatctgg gcctgacggt agaaaccatc agccgtctgc
    14941 tgggtcgctt ccagaaaagc ggtatgctgg cagtcaaagg taaatacatc accatcgaaa
    15001 ataacgatgc gctggcccag cttgctggtc atacgcgtaa tgttgcctga tttttccgca
    15061 taactcacta tccttctgtc atatcattaa atttttctga tttattgatc tggcagaagg
    15121 ttcatcactg tttcattcac cagatatggg ttaatctttt aattacaaac tgcgttgaca
    15181 gttgttgtaa ggagaccctg tatggctatg tatcagaaca tgctcgttgt tatcgatcct
    15241 aaccaggacg accaaccagc attgcggcga gctgtttatt tacatcaacg gattggtggc
    15301 aaaattaaag cctttttgcc gatctatgac ttctcatacg aaatgaccac cctgctctcc
    15361 ccggacgaac gtacggctat gcgtcagggc gtcatcagcc agcgtacagc ctggatccac
    15421 gagcaggcaa aatattatct caatgctggc gttcccattg aaattaaagt ggtctggcat
    15481 aaccgtcctt tcgaagccat cattcaggaa gtgatcagcg gcgggcacga tttggtgcta
    15541 aaaatggcgc accaacatga ccgtctggaa gcggtgattt ttacgccaac ggactggcat
    15601 ctgttacgca aatgcccaag cccggtgtgg atggtgaaag accagccgtg gccggaagga
    15661 ggcaaggcgc tggtggcggt gaatctcgcc agtgaagagc cgtatcataa tgcgctcaat
    15721 gaaaaactgg tcaaagagac gatcgaactg gcagaacaag tcaaccatac cgaagttcat
    15781 ctggttggcg cttatccggt aacgccaatt aatatcgcga ttgaactgcc ggaatttgat
    15841 ccgagcgttt ataacgatgc cattcgtggg caacatttgc tggcaatgaa agctctgcgg
    15901 cagaaattcg gcattaatga aaacatgacg cacgtagaaa aaggtctgcc agaagaggtg
    15961 attcctgatt tggcggaaca cttacaggcg gggattgtgg ttctgggcac ggtaggacgc
    16021 accggtattt cagcagcatt cctcggcaac acggcggaac aggtgattga tcatcttcgc
    16081 tgcgatctgt tggtcattaa acctgaccag tatcagacac ccgttgaact ggatgacgaa
    16141 gaagacgatt aacgactctt caataactga caaggcccga gttatcgggc ctatttttta
    16201 cttactgctc ttcgccgcca aggaaataaa ttcccaacgg gattgccagc aacacagtaa
    16261 agaccaggct ataaacaaaa atcatcgatg actgaatcac atacattgat gtcgtccact
    16321 cagacagcgg aatattgtac tgttctataa cgccgccgat agtcgcgcga gtcacaaccg
    16381 acgccgcaat aatgaaaacc gccaacaaac caaagagaaa tttcttccct ttcgctgact
    16441 tcagtttcgc cataatcatg atgtaatcct tgtgcagaca aatttcttgt tacctaaacg
    16501 taacaatacc atgacatttc accgttgtct gctctgaaaa cctcacctct tagctggatt
    16561 tacaatcaac gcattgatta gttagctaat gttgctactt ccttccctga ctattgatga
    16621 tctgaatgaa ttgtcgcggt aaaatagccc cgccaggggt ttccggcatg acgacaatta
    16681 tctggaggag cctgataaaa caatgatcgc tgaactgttt acaaataatg cgcttaatct
    16741 ggtcattatt ttcggtagct gcgcagcatt gattctgatg agcttttggt ttcgccgcgg
    16801 aaatcgtaaa agaaaaggtt ttttattcca tgcggtgcaa tttttaatct acaccataat
    16861 tatcagtgct gttggtagca ttattaatta tgttatagaa
//
LOCUS       DAPPNJ010000099        16457 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000099 DAPPNJ010000000
VERSION     DAPPNJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.099"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..292)
                     /locus_tag="RPM73_002653"
     CDS             complement(<1..292)
                     /locus_tag="RPM73_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6520999.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFH"
     gene            complement(331..1560)
                     /locus_tag="RPM73_002654"
     CDS             complement(331..1560)
                     /locus_tag="RPM73_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX6521000.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1726..2088)
                     /gene="ssrA"
                     /locus_tag="RPM73_002655"
     tmRNA           complement(1726..2088)
                     /gene="ssrA"
                     /locus_tag="RPM73_002655"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2303..2785)
                     /gene="smpB"
                     /locus_tag="RPM73_002656"
     CDS             complement(2303..2785)
                     /gene="smpB"
                     /locus_tag="RPM73_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDX6521001.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            2956..3393
                     /gene="ratA"
                     /locus_tag="RPM73_002657"
     CDS             2956..3393
                     /gene="ratA"
                     /locus_tag="RPM73_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDX6521002.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3383..3673
                     /locus_tag="RPM73_002658"
     CDS             3383..3673
                     /locus_tag="RPM73_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDX6521003.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(3735..4076)
                     /gene="bamE"
                     /locus_tag="RPM73_002659"
     CDS             complement(3735..4076)
                     /gene="bamE"
                     /locus_tag="RPM73_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDX6521004.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(4225..5886)
                     /gene="recN"
                     /locus_tag="RPM73_002660"
     CDS             complement(4225..5886)
                     /gene="recN"
                     /locus_tag="RPM73_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDX6521005.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(5972..6850)
                     /gene="nadK"
                     /locus_tag="RPM73_002661"
     CDS             complement(5972..6850)
                     /gene="nadK"
                     /locus_tag="RPM73_002661"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDX6521006.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            6973..7563
                     /gene="grpE"
                     /locus_tag="RPM73_002662"
     CDS             6973..7563
                     /gene="grpE"
                     /locus_tag="RPM73_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDX6521007.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            complement(7598..8203)
                     /locus_tag="RPM73_002663"
     CDS             complement(7598..8203)
                     /locus_tag="RPM73_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDX6521008.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(8326..9612)
                     /locus_tag="RPM73_002664"
     CDS             complement(8326..9612)
                     /locus_tag="RPM73_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDX6521009.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(9633..10424)
                     /locus_tag="RPM73_002665"
     CDS             complement(9633..10424)
                     /locus_tag="RPM73_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDX6521010.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            10591..11952
                     /gene="ffh"
                     /locus_tag="RPM73_002666"
     CDS             10591..11952
                     /gene="ffh"
                     /locus_tag="RPM73_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDX6521011.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            12089..12337
                     /gene="rpsP"
                     /locus_tag="RPM73_002667"
     CDS             12089..12337
                     /gene="rpsP"
                     /locus_tag="RPM73_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDX6521012.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            12356..12904
                     /gene="rimM"
                     /locus_tag="RPM73_002668"
     CDS             12356..12904
                     /gene="rimM"
                     /locus_tag="RPM73_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDX6521013.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            12935..13702
                     /gene="trmD"
                     /locus_tag="RPM73_002669"
     CDS             12935..13702
                     /gene="trmD"
                     /locus_tag="RPM73_002669"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDX6521014.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13744..14091
                     /gene="rplS"
                     /locus_tag="RPM73_002670"
     CDS             13744..14091
                     /gene="rplS"
                     /locus_tag="RPM73_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDX6521015.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14168..14650)
                     /locus_tag="RPM73_002671"
     CDS             complement(14168..14650)
                     /locus_tag="RPM73_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDX6521016.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14666..15892)
                     /gene="dgcN"
                     /locus_tag="RPM73_002672"
     CDS             complement(14666..15892)
                     /gene="dgcN"
                     /locus_tag="RPM73_002672"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDX6521017.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTDCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(15882..16400)
                     /locus_tag="RPM73_002673"
     CDS             complement(15882..16400)
                     /locus_tag="RPM73_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDX6521018.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         4117 a         3860 c         4229 g         4251 t
ORIGIN      
        1 agtgaaaggc ataaactaag tatacagcta aagtagccgt tccaattgtt ggtccaatat
       61 cgccttgatc atgaggttca aggtttacat tgaacttgaa gattagccat tcaaatcccc
      121 aagtaaaaaa aacaactaat atcatggctg cgaataattt cgcgatgtta tctgctgatg
      181 gattcatcag aacagattgt ttctgaaaaa attcacaaag tgagagtccg aaaaaaataa
      241 aaaaaagtgg tctggaacta aatttttcaa aaactttgaa tagggtttcc attttcttta
      301 ccttcaggat tatattagct attctgatac ttaggctacc agagcatttg atctttgagc
      361 ttgaacgtaa tcactccacc attgcatcaa actctgtcgc tctatcagat attctgcacg
      421 attgtatgct gcgataattt catctttttt cgagtgggca agcgctgcct caagaacttc
      481 agctctgaat ttaccagact cctctgccgc tgttcgtgca atagaacgca taccgtgagc
      541 tacaagctcg cctccgaacc ccattcggat gatagctgcg ttggctgttt gttcatgcat
      601 atgattaaga ggcgctttta tgctggggaa aacccattct ctatgcccac ttattgattt
      661 cattaattca aggatgcgca aagcttcttt actcaaagga actttgtgaa gctttttcat
      721 tttcatgaaa tcagcaggaa tgttccaaat gctgttggtt gtatcaatat cagaccacct
      781 tgcgcgaacg gcttcacccg gacgaaccca tgtcaacaat tgccattcaa ttagcatacg
      841 tgtttccaac cggattgacg cattcgtcaa agattccata aaccttggca attcgcttgg
      901 gggaagggca ggcatatttt gcttttttgg tttactgaat ctttgaccaa ggttgtcagc
      961 cgggttgaac tcaataagtt cttcagtagc tgcccaccgg aagatttcat tcagacgtga
     1021 aatgatacgg cgtagagttt ccaatacgcc tcgttgctca ataggatcaa ggtgttgttt
     1081 taagagctta ggtcggatct cattgatagg gacattaccc agaccgggaa agacatttct
     1141 ctctaagctg cgccagatgt ctgctgcatg gtcttgtgag atacctgatg tctttacctt
     1201 ctcatctaac catttccgcg ctacggcttg gagagtgtgc tcagtagcac tctttaatgc
     1261 cttcgcctta tcgttgttat ggatttgggg atcaacacca tttgccagaa aggagagata
     1321 ttcatcacgt aaggctctgg ctcttgcaag ggtaaggtga ggatatgtcc caaggctcat
     1381 tttggttctt ttcttgctca ctggtactgc atacctgaaa taccaatttt tcttgcctcc
     1441 tttcgccaaa ggagcgattc gtagaatcag accatcaccg tcaaacaagt tgatttcttt
     1501 atcggctggc ttggtgcttt tgatttcagt gtcagtgagc ttcttagcga tttttgccat
     1561 tttgggaccc tcggtttttg gacccttctt agtgggtccc attcagggtg ccataactcg
     1621 tagttctcag caattctcac tggacgacaa tagacgtaaa aaagcccgca gagcttgtgc
     1681 tgtgtgggct tagtagactt cattgaactt caaacaacta aaaagtggtg gagctggcgg
     1741 gagttgaacc cgcgtccgaa attcctacat cctcggtact acatgcttag tcagtcttta
     1801 cattcgcttg ccagctgcgg acggacacgc cactaacaaa ctagcctgat taagttttaa
     1861 cgcttcaacc ccaggcaggg cttccacgcg atctcttttg ggtttgacct ctcttgatcc
     1921 ccgtcctaag agcggaggct agggagagag ggctctaagc aggttattaa gctgctaaag
     1981 cgtagttttc gtcgtttgcg actatttttt gcggcttttt acgaggccaa ccgcccctcg
     2041 gcatgcacct tgggtttcgc aaatcccgtc gaatccagaa tcagccccaa tgtgtaaagg
     2101 taagtatacc agatttatga gcgccatgac cagcctcaat ggcgttatcg ttaaagattt
     2161 agcacccatg tagcctgatt tttattcgat taagcaatgg gatggcaaca tttgtgtcgg
     2221 atgtgatagc caataagatg ttcattcgcg ccgccggaga gggaggcgcg gtgaggaact
     2281 ggtcaataat tggagtgcag gtttaacggt gggcgttttt catgatacgt gctttatcca
     2341 cctgccattc gcgttctttg atatctgaac gtttatcgtg ctgtttttta cctttggcga
     2401 cgccgatttt cactttgcac caggcatttt tccagtacag ggagagcgcc actacggtat
     2461 agccttctcg attgacgcga ccgtacaatg agtccagttc gcgctggttg agaagtaact
     2521 tgcgggtacg ggtaggatcg cacaccacat gcgtggaggc cacggccatt ggcgtgatgt
     2581 tagcgccaaa cagaaatgcc tctccgtcac gcagaaggac gtagctgtcg ctgatattgg
     2641 cttttcctgc gcgcagggat ttaacctccc agccttgcag ggcaagtccc gcttcgaact
     2701 cttcttcgat aaagtattcg tgacgggcgc gcttgttaag cgcgatggtc gctgaaccag
     2761 gtttatgtgc ttttttcttc gtcataagcg tcgtgaatca tcggtaatct gaaatcgaaa
     2821 acaccccata tcaatcctgc gggggacaag gctctatctt agcatgaacc cgatgttacc
     2881 cagcgccggg atagcgtttt ttttacagca ggataaatga tattatttgt tggatttttg
     2941 ttgatggaaa ttgttatgcc tcagattagc cggaccgcac tggtacccta cagcgcggag
     3001 caaatgtatc agttagtgaa tgacgttcag tcttatcctc agtttttgcc gggttgtacc
     3061 ggaagtcgga ttctggagtc cactcctggg cagatgactg cggcggtaga tgtctctaag
     3121 gctgggatca gcaaaacgtt tactacccgc aaccagttga ccagtaacca aagtattctt
     3181 atgaatctgg tggatgggcc gttcaagaaa ttgattggtg gatggaagtt tacgccgctg
     3241 agccaggagg cgtgtcgtat cgagtttcat ctcgactttg agtttaccaa taagttgatt
     3301 gaactcgcct ttggtcgcgt gtttaaagag ctggcgtcta atatggtcca ggcttttacg
     3361 gttcgtgcga aagaggttta cagtgccagg taaaattgcc gttgaggtgg cttatgcgct
     3421 acctgagaag cagtacctgc agcgcgtgac gctgcaggag ggcgcgacgg ttgaagaagc
     3481 tattcgcgcc agtggcttgc tggaattgcg taccgatatc gatttaacta aaaacaaagt
     3541 cggcatttac agccgtccgg caaaactaag cgatagtgtg catgatggcg atcgggtgga
     3601 gatttatcgc cctctcattg ccgatccgaa agagcttcgc aggcaacgag cagaaaaatc
     3661 agcgaataaa taacagacag aaaaaaggtg ctcattgagc acctttttta acgtctttga
     3721 gagcaacttt attattagtt accactcagc gcaggtttgt tatcaatatt ggtcaacaca
     3781 ccgctactgt taaaggtcag cgtcagcgtt tgctgcgtta caccttcatg acctggttgc
     3841 tggcggaaga catagaacca ggtattcgta ccaaatggat cggacatcag cggtgtaccc
     3901 aatgcgtacg caacttgttg ttgcgtcatg ccaacacgta ttttggatac gtcgttagcg
     3961 gtcagatagt tcccctggtt gatgtcagga cggtaaacca ctcgctccag agtggaacag
     4021 cctgcggtca acatcaatag tactgctgct gcagcagtca gcgttttaca gcgcatagtg
     4081 atttgattcc ttttcgggcc cgagcagtac gtggctcatc tgtaatatgc cgatgataat
     4141 agacctttca tcactttaaa accttttgct ttccggtctt acggcgtttt gctgtttact
     4201 ctgaccgtga agcaggaaaa aagtttacgc tgcaagcagt tctttcgcat tcgccagtgt
     4261 attacgtgtg acttcactgc caccaagcag gcgcgccagc tcttgtaacc gcgctttttt
     4321 attcagggat tgcatatgcg tttctgtcat cgcaccatcg gtttctttgc tgacaaaata
     4381 gtgttgatga ccacatcccg cgacttgtgg caggtgggta acacacatca cctgagttga
     4441 ttcgccgagt tgacgcagca gtttgccgac aactgccgct gttggaccgc taatccctac
     4501 atccacttca tcaaaaatca gtgccggggt ttccatttta cgcgccgtga tgacctggat
     4561 tgccagtgcg atgcgggaca attcaccacc ggatgcgact ttggcaatag gctgcattgg
     4621 ctgacctggg ttggtggtta cccgaaactc aatacgatcg gcaccgtcag cgcccaggtg
     4681 atgctcgtca aatttaacat cgatcgtaaa ctgcccatgc ggcattgaga gcgcatgcat
     4741 actgtcggtg atcagctgtg caagttcttc tgcatattgc tggcgttgtt ggtgtaatgc
     4801 gcgcgcgatt tccagtgcct gctgatgatg tttcgttacc gccagcgcaa gcgtttcttg
     4861 tgagtcggcc tgatcgtcca gttgctgctg ttcttccagt agcgactggt aatactgtgg
     4921 caatgcctca gggctgacgt gatgtttacg tgccagcgaa atctgttttg agatgcgctg
     4981 ttcaagttca aatagtcggt tgggatcgag atccagacga tcgcagtagt ggcgcagttc
     5041 atcgctggct tcagcaatct ggatggtagc ttcttccagc atatcaagta cgccggacag
     5101 tttgctgtcc atgccaatca attcgctcac cagttgttta gccgtgtaaa gctgactttg
     5161 caggtttgcg tcttcaccgt cggccattaa tgccaatgca ttctggctgg tggtcagcaa
     5221 ttgaccgctg ttcgccagac gtttgtactc ttcgtcgatt tgttcaaact ctccgggctg
     5281 cggattaaat tcgttaagtt cttttaattg gtattgcagc agttccgcac gggcagcgcg
     5341 ttcctgactt aactgttgat gatgcgcgag gtcacggcag ctttgatgcc acaactgata
     5401 acgtgctgtc atttcctgga gtagagaggt ttcattggca tagccatcaa gcaggaactt
     5461 ttggtgctcg ggtttggtga gtaactgatg agcgtgctga ccatggatct gaatcagcaa
     5521 ctgacccagt tcgcgcagtt gtgacagagg aacagctgta ccgttgatga aaccacggga
     5581 gcgaccatcg ctgctgatca cgcgacgaag caaacattca tgcccgtctt caagctggtt
     5641 ttcttccagc cagcgcagag ccgctggcgt atctttcaga gaaaaacggg cgcacaggtc
     5701 agcgcgagca gcgccggtac gcaccatgtc ggcttcagcg cgaccaccga gacaaagacc
     5761 gagggcatct attgcaatag atttacccgc gccggtctcg ccagttatta cggtcatgcc
     5821 gctatgaaaa tcaatctcaa gctcacgaac gatagcaaag ttgctgatgg tcagttgtgc
     5881 caacatagtc gttttcctgt atgaaaaacc attactgtta ttgtgtacag tataaactgg
     5941 ttttatatac agtaaagagg ctggcgtaaa attagaataa tttttttgac cagccgagct
     6001 tggtgcttaa tgtgttgaaa taactgtaat ctttcggatg aatcagattc agatggtaat
     6061 cacagcgacg aatcaggaca tcttcacctt cctgaatcgg cagtgctatc tggctgtcgc
     6121 aactgatttc caggtcgtta cggcgatgcg aaaaacgcag acggatcgtg ctgctgctgt
     6181 ttatgaccag tggtcgtgct gacaacgtat gcgggaacat gggcaccagg gtaatcgcat
     6241 ccagagaggg ggtcagaata ggaccgcctg cagagaggga ataggcggtg gagcctgttg
     6301 gcgtcgaaat aattagtcca tcagatcgct gagaaaacgc aaagatctcg tcgatataca
     6361 cttcgaactc aatcatatgc gccactttgc ctggatgaag caccacttca tttatcgcgg
     6421 tgctgatgcg tttctggcaa tcttgctgac agacttgcgc ttccagcaaa aaacgtttct
     6481 cgctgatgta gtggccttcc agcacatcgg ctaactgttg ctgggcgtta tcggggtcaa
     6541 ggtcagtcaa gaaacccagg ttgccacggt tgattccaat aactttaata tcgtaacggg
     6601 cgagtgtgcg cgccgcgccc agcatattac cgtcgccacc aacgactacc gcgagatcag
     6661 ctagttgccc aatctccgcg agcgtgccag ttttcacatt cttcagttgc agttcgtgag
     6721 cgatttgttg ctcaacgatg acctcgtaac cttttgtgca cagccagcgg tagagcattt
     6781 catgtgttgt cagtgcagta gggtgccgtg ggtgtcccac aatgccaata cacttgaaat
     6841 gattattcat ttttccgagg tccttgttgc gaagattgat gacaatgtga gtgcttccct
     6901 tgaaaccctg aaactgatcc ccataataag cgaagttagc gaggtgaatg cgaaaaaaac
     6961 gcggagaaat tcatgagtag taaagaacag aaaacgcctg aggggcaagc cccggaagaa
     7021 attatcatgg atcagcacga agagattgag gcagttgagc cagaagcttc tgctgagcag
     7081 gtggatccgc gcgatgaaaa aattgcgaat ctcgaagctc agctggctga agcccagacc
     7141 cgtgaacgtg acggcatttt gcgtgtaaaa gccgaaatgg aaaacctgcg tcgtcgtact
     7201 gaactggata ttgaaaaagc ccacaaattc gcgctggaga aattcatcaa cgaattgctg
     7261 ccggtgattg atagcctgga tcgtgcgctg gaagtggctg ataaagctaa cccggatatg
     7321 tctgcgatgg ttgaaggcat tgagctgacg ctgaagtcga tgctggatgt tgtgcgtaag
     7381 tttggtgttg aagtgatcgc cgaaactaac gtgccgctgg acccgaatgt gcatcaggct
     7441 atcgcaatgg tggaatctga tgacgttgcg ccaggtaacg tactcggcat tatgcagaag
     7501 ggttatacgc tgaatggtcg tacgattcgt gcggcgatgg ttactgtagc gaaggcgaag
     7561 taaatatctg atggccgacg cgaagtcggc cattttttta gcttaaaaaa tcctgtactt
     7621 ccagccacgg cgtgttgaaa tcatctattt ttttatattc gttcattggc agaacgacca
     7681 cgttataggt gtcagatcct tcattcagat caaaaatctg aaagcccctt ttattgagtt
     7741 cggtttggat gcgttcaaag atttcgtcaa ggtagcggtc tccggcttca cagacatcgg
     7801 agatttcatc ctcttgggtt atttcgacaa gaagactcct taattcctca gaagctatca
     7861 ggttttttgt gagctcataa aagcgatctg cagcaaaatt agccagctca ccttcttcat
     7921 cttcaccttt ccagtcaacg agttgaatta atccttcacc gcgtagaacc tgtaaaaatg
     7981 cctgcgccag gtcacgattc ccgagatcgc caccactgca atcgagaacg tcctgcaatc
     8041 catattcgtc ttcatcttcg tcttcaaaga tttcaggttg cgtcgaaagt tgagagaaaa
     8101 tagtgggcca taaggttggg gtgtgctgta aaaaaacgct ggcgacgacg ttgagatcgt
     8161 ctgcaaaatc cttatcccat tcaattacat aatcttctga cataacatca atatcctttc
     8221 cgaatgcttg aaaagacatt attacattgc taagtaaatg acaggaattg atggaatcct
     8281 aaaagacggc gataaaaaaa aggcgaaccg gagttcgcca ttgcgttact ccgccacact
     8341 ctcgcgcagc ggtttcacag gaaaaacttt tacctgctta atcatattgt cctgtacgtc
     8401 gagaatatct atatcgtact cgccaatacg cacgcgggtg cctgcgacag ggatctcttc
     8461 cagtgcctca agaatgacgc cattaaccgt gcgggcatca tcttccggta gatgccagtt
     8521 aaaggctttg ttgatttccc gcacgttggc ggtgccatcg ataatcaccg aaccgtcgtt
     8581 ttgcggcgtg acctcttcgg caagtgttgg cgacatcgac gtagtgaaat cgccgacaat
     8641 ctcttccaga atatcttcaa ccgtcaccag cccctgaatg tctccatact cgttgacgac
     8701 caggccgact ttctttttgt tgcgctgaaa ctttaccaac tgcgtgctga gcggcgtacc
     8761 ttccggcaca aaatagatct cgtccgcggc gcgcagcatg gtttctttgg tgaactcttt
     8821 tttctccgac atcaaccgcc aggcttcacg tactcgcagc atactgatgg cgtcgtccag
     8881 cgaatcacgg tagagcacga tgcgcccgtg aggtgagtgg gagagttggc gcagaatcga
     8941 tttccagtca tcgttgatat caataccgat aatttcactg cgcggcacca tgatgtcatc
     9001 aacggtcatt ttttccagat cgagcaccga cagcagcata tcctgattgc gacgggaaat
     9061 ttgtgagcgt gattcgtgca cgatagtgcg caactcttct ttgctcaaag agccgctaac
     9121 cacgatatcg gttttgatac ccatcatgcg catcagcata cgggtgatag cattcagcaa
     9181 ccagaccagc ggcatcatca aaatttgcag cggagccagc agaaaactac tcggataagc
     9241 gactttttcc gggtacagcg cggcaatggt tttcggcaat acctcagcaa ataccagtac
     9301 gacaaaagtc agcacaccag tcgcaattgc cacgcccgca tcgccgtaca aacgcatccc
     9361 aacaatagtg ccgagcgcgg aggcaagaat attgaccagg ttattgccga ttaacaccag
     9421 gcttatcagg cggtctggct tacgcagcaa tttttcgacg cgtttggccg agcgattacc
     9481 ctgtttcgcc atatgtcgca gacgatagcg gttgagggtc atcattccgg tttcggaccc
     9541 ggaaaaatag gctgaaatga ccaccatgat gatcagaata atgatcaacg tagtagtaga
     9601 aatgtgttcc aggggaaact ccttttctgg gtttagctga ttaactgctg gacaattcgg
     9661 ctgccgaagt aggccagtgt cagaattacc gcgcccgcaa cgttaaacca gacgacgcgg
     9721 cgtccacgcc atccttcatg gtaatgtccc cacagcagca caatatagac aaaccacgcc
     9781 acgatagaga gcacagcctt gtcgatattt tccatgctaa ataggttgtg catgtagaac
     9841 aggccagtgc aaagcgtgag cgttagcagc accacgccaa tctgcgtgat gtggaacatt
     9901 ttacgctcga tactcatcaa tggcggcatt tcctggttaa atgccagctt cttgttcttc
     9961 agttggtaat caatccacgc cagttgcagc gcgtacaggg cggcgataat tagcgtggca
    10021 taggaaaaga gcgataagcc aatgtgcacc agcatcccag gcgttgcttc cagatgggtg
    10081 atgtattcat tgggcatgaa ggtcgccagc gccaggttga taagcgcaaa ggcatagaca
    10141 atgggtagca gcaaccagcc acgattgcga gaagccacaa tggtcattac cgtacagatc
    10201 atcaaactga ccaatgaacc aacgttcagc aggctgaggt tttgtccgct atcaccgtcg
    10261 ggcaggatgc gggcttccag agcgattgcg tggcagacca gcgcaatgac cgcagaaata
    10321 atagccatgc gccgccagcc gccgtttttt tgcagcagac cgggaacaat cagcgcaaga
    10381 ctgacggagt aggcgacaag cgcgagcaga gcaaaaacgg gcatagtgat gtcgacagtt
    10441 tgagcaataa gaaagagaag cagtatagcg tcaggtgaac gctgctccaa ccgttgcata
    10501 acaacaaaga cgccttcatg ttatactgcg gcaaaatact gatgatgtgt cgcgattgcg
    10561 gccaaccgtt tccaccccag gcgagagaca atgtttgata atttaaccga tcgtttgtcg
    10621 cgcacgctgc gcaatatcag tggccgtgga cgcctcactg aagacaacgt aaaagatacg
    10681 ctgcgcgaag tgcgcatggc gctgctggag gcggacgtcg ctctgccggt agtgcgtgag
    10741 tttatcaatc gcgtaaaaga gaaagcggtt ggtcatgaag ttaataagag cctgacgccg
    10801 gggcaggagt tcgtcaaaat cgtccgtaac gaactggttg cggcgatggg cgaagagaac
    10861 cagaccctga acctggcggc gcaaccgcct gcggtcgtgc tgatggcggg cctgcaaggt
    10921 gccggtaaaa caaccagcgt tggtaaactc ggtaagttcc tgcgcgagaa gcacaagaag
    10981 aaagtgctgg tggtttctgc cgacgtttat cgcccggcgg caatcaaaca gcttgagacg
    11041 ctggcagagc aggtgggcgt tgatttcttc ccttctgatg ttggtcagaa gccggtagat
    11101 atcgttaacg cggcgctgaa agaagccaaa ctgaaattct acgacgtgct gctggtggat
    11161 accgctggtc gtctgcacgt tgacgaagcg atgatggacg agatcaaaca agtccatgcg
    11221 tcgattaacc cggttgaaac cctgtttgtg gttgacgcca tgaccggtca ggatgcggcc
    11281 aatacggcaa aagcattcaa tgaagcgtta ccgcttaccg gcgtagtgtt gaccaaagtg
    11341 gacggcgatg cccgcggcgg tgcggcgctc tctattcgtc acatcactgg caaaccgatc
    11401 aagtttctcg gtgttggcga gaaaactgag gcgctggagc cgttccatcc ggaccgcatc
    11461 gcgtcgcgta ttctcggcat gggcgacgta ctgtcgctga tcgaagatat cgaaagcaaa
    11521 gttgaccgcg cgcaggcaga gaaattagcc agcaagctga aaaaaggcga cggcttcgat
    11581 ctcaacgatt ttcttgagca actgcgccag atgaaaaata tgggcggcat ggctagcctg
    11641 atgggcaagc tgccgggcat ggggcagatc ccggataatg ttaagtcgca gatggacgat
    11701 aaagtgctgg tgcgtatgga agccatcatc aactcgatga cgatgaaaga gcgcgctaag
    11761 ccagaaatca tcaaaggttc gcgtaaacgc cgtattgctg ccggttgcgg tatgcaggtg
    11821 caggacgtta accgtcttct gaaacagttc gacgacatgc agcgcatgat gaagaaaatg
    11881 aagaagggcg gaatggcgaa gatgatgaga agcatgaagg ggatgatgcc gccgggattt
    11941 cctggtcgct aaatgacctt atgcgacaga gttattaact gctgattgca ttttctccag
    12001 aaatcagtaa aattttcggg cttttaatat gacaccggac tccgttcctc gatggggtcc
    12061 ggttgtttta ttcacacaag aggatgttat ggtaactatt cgtttagcac gtcacggcgc
    12121 taaaaagcgt ccgttctacc aggttgttgt cgctgacagc cgtaatgcac gcaacggtcg
    12181 cttcatcgag cgcgttggtt tcttcaaccc aatcgctagc gaaaaagaag aaggcactcg
    12241 cctggatctg gatcgcatcg ctcactgggt tggccagggc gcaactattt ctgatcgcgt
    12301 tgctgcgctg atcaaagaag taaacaaagc agcttaatct gtcacggtgg tcatgatgag
    12361 caaacaactc accgcgcaag cacctgttga tcccatcgtt ttgggaaaaa tgggttcgtc
    12421 ttacggtatt cgtgggtggc tcagagtgtt ttcttccacc gaagacgccg aaagcatttt
    12481 tgactatcag ccctggttta tccagaaggc gggtcagtgg cagcaagtcc agctggaaag
    12541 ctggaagcac cacaatcagg acatgatcat caagctgaaa ggcgttgacg atcgtgatgc
    12601 ggcgaacctg ctgacgaatt gtgaaattgt cgtggattca tcgcagctgc ctcagcttga
    12661 agagggtgac tactactgga aagacctgat gggctgccag gtagtaacca ctgaaggcta
    12721 cgatctcggt aaagtcgtcg atatgatgga aaccggatct aatgacgttc tcgtcattaa
    12781 ggcaaacctg aaagatgcgt ttggtatcaa ggaacgtctc gtaccgttcc tcgatgggca
    12841 ggttatcaag aaagtcgatc tcactactcg ttcaatcgaa gtagattggg atcctggttt
    12901 ttaaaccacc ggataaacgg taaaagacgg cgctatgtgg attggcataa ttagcctgtt
    12961 tcctgaaatg ttccgcgcaa ttaccgatta cggggtaact ggccgggcag ttaaaaatgg
    13021 cctgctgagc atccagagtt ggagtcctcg cgacttcacg catgaccggc accgtaccgt
    13081 ggacgatcgt ccttacggcg gcggaccggg gatgttaatg atggtgcaac ccttgcggga
    13141 cgccattcat gcagcaaaag ccgcggcggg tgaaggcgca aaggtgattt atctgtcacc
    13201 acagggacgc aagcttgatc aagcgggcgt cagcgaactg gcaacgaatc aaaaattgat
    13261 tctggtgtgc ggtcgctacg aaggtataga tgagcgcgtg atccaaaccg aaattgacga
    13321 agaatggtca atcggcgatt acgttctcag tggtggtgag ttaccagcaa tgacgctgat
    13381 tgactccgtt tcccggttta ttccgggagt actgggacat gaagcctcgg caacggaaga
    13441 ttcctttgct gaaggattgc tggattgccc gcactatacg cggcctgagg tgttagaagg
    13501 gatggaagtt ccgccagtgt tactgtcggg caaccatgcc gagatacgtc gctggcgttt
    13561 gaaacagtcg ctgggccgta cctggcttag aagacctgaa cttctggaaa acctggctct
    13621 gactgaagag caagcaaggt tgctggcgga gttcaaaacg gaacacgcac aacagcaaca
    13681 taaacatgat gggatggcgt aagcccccga gatatcagtt tacccaggat aagagattaa
    13741 attatgagca acattattaa gcaacttgaa caagagcaga tgaagcagga cgtaccttcc
    13801 ttccgtccgg gtgataccgt ggaagtgaaa gtatgggttg ttgaaggttc caaaaaacgt
    13861 ctgcaggcat tcgagggcgt ggttatcgct attcgtaacc gcggtctgca ctctgcattc
    13921 actgttcgta aaatttccaa cggcgaaggc gttgagcgtg tcttccagac tcactctccg
    13981 gtagttgaca gcatttctgt caaacgtcgt ggtgctgttc gtaaagctaa actgtactac
    14041 ctgcgtgagc gtactggtaa ggctgctcgt atcaaagagc gtcttaacta agatttcgct
    14101 taagcgacat cctgttaaga agggctggcc aattggctgg cccttttttt gtctgtttgc
    14161 tcgtgtttta aggggttgta atcaccactg cgacgcggcg gttctcggcg cggccctggg
    14221 cggtcttgtt actggctatg ggatattttt ttcctaaacc ctgtgtggtg agattgctgc
    14281 gtggaatttg tccacccata gcccatgcat cggccacgac attcgcccgt ttcaatgata
    14341 agccttcgtt gtaactgtct tcaccatagt tatcggtgtg tccatccata cgggcatgtg
    14401 ttagccctgt cgaggccaat ttggctgcca tggtttggat ctgttgctgg ctttccggga
    14461 gcaatttgta gtcatttttt gcgaacagaa tggcatctga taagcccagc gaccagtcgc
    14521 cggcggattc agtaaatcca taagattgca tagcggcgac ttgctcagga gtaaactttc
    14581 cctgagggga ctggcagcca gtcagtatta gtgaggtgaa aatcaggggt gctaccaggt
    14641 gctttatcat atcgatatat ccttgttatc tcaccagctt ttcggcacgc tggtgtttgg
    14701 cctgatacat attgtgatcg gcaagctctt gtaatttttc cgcagaggcg tgctcaatgg
    14761 tcatcgcgta accaatgctt aatgtcatgg tggtctgatg gccattatga agatcaaacg
    14821 ggagattaaa gatttgtgtc agtgctgagc atatctgctg cacttcagat tccgactgta
    14881 catcatagag caccatagcg aattcatcgc cgcccaggcg gtatgcttta tgtcgcagcc
    14941 cgccaaattc agctaaccgt tttgcgattt caatcaagac tctatcgccc gtcgcatgac
    15001 cccaggtatc attgatatat ttgaaattat cgccatcaag aaatagtaac gccgacgttt
    15061 ttcgggcatc ggaattgttc atcaacgtat tgatgccgct acgaaacgct gcgcggttag
    15121 ccagcccggt taatgggtca tgtagcgcgg tacgcagaag ctgcgcattt ttggcctgta
    15181 aacgaagctg ccactcttcc atttcatcca gcagactgtt gaagtcgaga gcgaagcggt
    15241 gaaactcagc gatacgttct tccgaaactc gtcgggaaaa attgcggttg gaacgcacat
    15301 catgtacgac atcggtgata tttttcagcg cttctaccag gccattgtgc aaatggcggg
    15361 tgagggtaat tgcgatgcct gatgccagca gaatacaatc ggtcagtacg gcgagcgaaa
    15421 accagataaa atggctgatt gaactgtcgc gagcggttaa gcgtacttcg ccaatggttt
    15481 caccattgtg acgaatcggc tgaatgatgg gggcggggaa gagccagtgg cttatgaaat
    15541 tgctgaaagt atcgcctgga tccttacggg tgtaatgcca ggacgccaga atattttgct
    15601 gcttatcacg tacttctgca gttgaaaatt gcccttgctg gcccagcgct gccagtgttt
    15661 cagttgctgc agggccatcg gcaaaaacga cagctgcttc cagactgtaa gtcattgttg
    15721 ctgctgtcag tgccaggttt ttttgcgcgt actgtttcag ggtcagcacc gaggtcacgg
    15781 aaagcagcaa ccagatcagc atcatagtga taaatatgct ggtcatactg atgttgcgta
    15841 atgctctttt aaacgtgggg cgcttattaa gagaattatc gttatccatc atttttcttc
    15901 cgtgcgagca ttaagacatc cgggttgacc tttacaccgc tacgcgataa ggcatccagg
    15961 tttacggcaa atctgacgtc attattgtgg atgatcagac aaaaggcgct accaataatg
    16021 cactcggtat tttgttcggc aattaataat aacgcctttg acggatattg ttcggttaat
    16081 tccatctgga atgtcggtga ctcgttgcca aaataaaaac cattgcagcc tgaaatcatc
    16141 gcctcttgtt gagtgtgaat gatgacaggc agataaggca aagatgtcgc ggcattctct
    16201 tgtagggcgg tactaaaacg tgaagatgaa aagatacata actttggtgg ccctgatagc
    16261 gcaggccagc gggtgtaact gacaataccg gaaaccatca tacgcacgtt tttcgcaacg
    16321 tcagaaggct cctgagcgag tattggtgct cccgtcagca ggagaatcag aagtaagaac
    16381 agtcggtgag aaaaacgcat tcagggatcc ccaccatctt cgctcgaaat gcatcgatat
    16441 tttttcagaa tgctcat
//
LOCUS       DAPPNJ010000100        16324 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000100 DAPPNJ010000000
VERSION     DAPPNJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.100"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RPM73_002674"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RPM73_002674"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDX6521019.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="RPM73_002675"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="RPM73_002675"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDX6521020.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="RPM73_002676"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="RPM73_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6521021.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="RPM73_002677"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="RPM73_002677"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDX6521022.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="RPM73_002678"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="RPM73_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDX6521023.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="RPM73_002679"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="RPM73_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDX6521024.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /locus_tag="RPM73_002680"
     CDS             complement(8377..9201)
                     /locus_tag="RPM73_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDX6521025.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="RPM73_002681"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="RPM73_002681"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDX6521026.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMT
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="RPM73_002682"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="RPM73_002682"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDX6521027.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13134..13607)
                     /locus_tag="RPM73_002683"
     CDS             complement(13134..13607)
                     /locus_tag="RPM73_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDX6521028.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="RPM73_002684"
     CDS             complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="RPM73_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDX6521029.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14760..15758
                     /gene="yiaK"
                     /locus_tag="RPM73_002685"
     CDS             14760..15758
                     /gene="yiaK"
                     /locus_tag="RPM73_002685"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDX6521030.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15770..16237
                     /locus_tag="RPM73_002686"
     CDS             15770..16237
                     /locus_tag="RPM73_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDX6521031.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         4155 a         4130 c         4015 g         4024 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcaa tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaatgtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga tcgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 tgccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacactg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgtg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg aagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg atcttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacatgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttattataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcatgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat
     9841 tcagaacgcc gccgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcaca gtggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat gacggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat ttgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctt caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaagcac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ctgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 aattcataaa ccagaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcaa ctctaccagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttattag gcctacatga
    13021 tttctgcaat atatttaatt tgcaagaatt tgtgggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaaac gtttcaaaca
    13141 ggctgctgcc gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt
    13201 gtcgggcagg cttcaacaca ggccggtccc gtctcccgat gccagcacag atcgcactta
    13261 atcgccctcg cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc
    13321 atacagcttt tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg
    13381 cttatcgcgt caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct
    13441 actgccgtgg tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt
    13501 gacaacgcag cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaagta
    13561 cggcaaccga tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg
    13621 cactaaaaca gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat
    13681 gttaaagttt tggcacttgt ccaaagcgtt atgtgatcgc gccctggtcg tccctgacag
    13741 taaatcccag ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt
    13801 tctcccccac ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc
    13861 gcccatgaat attaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc
    13921 tgtccatcgc cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct
    13981 cggtaatggt attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact
    14041 tcacgtagtc cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg
    14101 gcatatgctg gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat
    14161 aaatcaaaat agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga
    14221 tgttcagtgc ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct
    14281 tctgcccgac ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg
    14341 tcacatagcc gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac
    14401 cagccagctc cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa
    14461 tcagcatcaa cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca
    14521 tctcgttctc tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg
    14581 caaagtgtgc cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc
    14641 ttttgaaatt catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag
    14701 tttcgcatat tgaaatttta agccaaaaaa gcgatcaaaa aaaaacaagg aagcctggga
    14761 tgaaagtgac atttgagcag ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg
    14821 ttgacagcga aacggctgac gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg
    14881 tttattctca cggcgttaat cgtttccctc gtttcattca acaactggaa aacggcgata
    14941 tcattcctga tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg
    15001 cccagcgttc gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc attgaactgg
    15061 ctgccgatca cggtattggt ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg
    15121 gcagctacgg ctggcaggcg gcggaaaaag gctatattgg catttgctgg accaactcca
    15181 tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg
    15241 tcgccattcc ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg
    15301 gcatgttaga agttaaccgc ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg
    15361 atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa gaatcgccgc attttgccga
    15421 tgggctactg gaaaggttct ggcatgtcga ttgtgctgga tatgatcgct actctccttt
    15481 ccgacggcgc atcggttgcc gaagtcaccc aggacaacag cgacgaatac ggcatttcac
    15541 aaatttttat tgccattgaa gtggacaagc ttatcgacgg tcccacccgc gatgccaagc
    15601 tgcaacgtat catggattac gttactaccg ctgaacgcgc tgacgaaaac caggccatcc
    15661 gcttacccgg ccacgaattt actaccctgc tggccgaaaa ccgccgtaac ggtattaccg
    15721 ttgatgacag cgtgtgggcc aaaatccagg cgttataagg agataagtca tgatttttgg
    15781 acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt
    15841 tctgcgcgct accgatttca acgccctgga gccgggcgtt gtcgaaatcg acggcaaaaa
    15901 tatttatgcg caaattatcg atttaaccac acgcgaagcg gtggaaaacc gtccggaagt
    15961 gcatcgtcgg tatatcgata ttcagtttct ggcgtggggt gaagagaaaa tcggcattgc
    16021 tattgatacg ggaaataata aagtcagcga atcactatta gagcagcgcg atattatttt
    16081 ttatcatgac agtgaacatg aatcatttat agaaatgata ccgggcagct acgcaatttt
    16141 ctttccgcag gatgttcacc gaccaggttg tattttgcaa acagcctctg agatacgaaa
    16201 aattgtggtt aaagtcgcat taacggcgct gaattaatcg aataatcgtc tacgccagaa
    16261 cgactgggta atgttattgc tctaactata ttaataattt aggttgctgc acatgcaact
    16321 tgaa
//
LOCUS       DAPPNJ010000101        16110 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000101 DAPPNJ010000000
VERSION     DAPPNJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.101"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(402..1598)
                     /gene="hemY"
                     /locus_tag="RPM73_002687"
     CDS             complement(402..1598)
                     /gene="hemY"
                     /locus_tag="RPM73_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDX6521032.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1601..2830)
                     /gene="hemX"
                     /locus_tag="RPM73_002688"
     CDS             complement(1601..2830)
                     /gene="hemX"
                     /locus_tag="RPM73_002688"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDX6521033.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2852..3592)
                     /gene="hemD"
                     /locus_tag="RPM73_002689"
     CDS             complement(2852..3592)
                     /gene="hemD"
                     /locus_tag="RPM73_002689"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDX6521034.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3589..4530)
                     /gene="hemC"
                     /locus_tag="RPM73_002690"
     CDS             complement(3589..4530)
                     /gene="hemC"
                     /locus_tag="RPM73_002690"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDX6521035.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4917..7463
                     /gene="cyaA"
                     /locus_tag="RPM73_002691"
     CDS             4917..7463
                     /gene="cyaA"
                     /locus_tag="RPM73_002691"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDX6521036.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7503..7823)
                     /gene="cyaY"
                     /locus_tag="RPM73_002692"
     CDS             complement(7503..7823)
                     /gene="cyaY"
                     /locus_tag="RPM73_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDX6521037.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            8286..8489
                     /locus_tag="RPM73_002693"
     CDS             8286..8489
                     /locus_tag="RPM73_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6521038.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8526..9350
                     /gene="dapF"
                     /locus_tag="RPM73_002694"
     CDS             8526..9350
                     /gene="dapF"
                     /locus_tag="RPM73_002694"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDX6521039.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9347..10054
                     /locus_tag="RPM73_002695"
     CDS             9347..10054
                     /locus_tag="RPM73_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDX6521040.1"
                     /translation="MKQPGKELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10051..10947
                     /gene="xerC"
                     /locus_tag="RPM73_002696"
     CDS             10051..10947
                     /gene="xerC"
                     /locus_tag="RPM73_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDX6521041.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10947..11663
                     /gene="yigB"
                     /locus_tag="RPM73_002697"
     CDS             10947..11663
                     /gene="yigB"
                     /locus_tag="RPM73_002697"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDX6521042.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11747..13909
                     /gene="uvrD"
                     /locus_tag="RPM73_002698"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11747..13909
                     /gene="uvrD"
                     /locus_tag="RPM73_002698"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDX6521043.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPAEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14056..14820)
                     /locus_tag="RPM73_002699"
     CDS             complement(14056..14820)
                     /locus_tag="RPM73_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDX6521044.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15190..>16110
                     /gene="corA"
                     /locus_tag="RPM73_002700"
     CDS             15190..>16110
                     /gene="corA"
                     /locus_tag="RPM73_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDX6521045.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPY"
BASE COUNT         3656 a         4222 c         4350 g         3882 t
ORIGIN      
        1 cattcataat aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagttatt
       61 actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt
      121 cccgattctc tgtggcataa taaacgggta gatgctcatt ccatctctta tgttcgcctt
      181 agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca
      241 gatgtcgctt atgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt
      301 tgtcatacag acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgcat
      361 cagaaaggtc ccggaggtgc ttgcctccgg gtgagaagga actactgtgg tgggttgttc
      421 tgcaacgtta acatcaaacc gtcgcgacgc atcgctgcag cttcttccgg cttgtgcagt
      481 ctgtccagcg cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg tttcagcgcc
      541 gcgcggaagg cgagcgatgc ttcctgccat tctccgtgct tcatcagtga ctggcccagt
      601 gtgctccaca acagcgggcg atcgccgacg tttttgatct gctggcgcag cactttttcc
      661 agctgttccg gattgttggt tttcagtcga ggaatcggca gcagtaggcg atcgtcgtac
      721 tggcgtttca ggccatcgat gataatttgc tgggcagtat catgatcgtc acattcaata
      781 agatgttccg ccattgccac ctgcaacgct acctgatgac gcgttttccg gctttggttt
      841 ttccaccagt tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc catcaggcca
      901 atccatgcct gttgttccag cattgcacga tgttcttcat caccaacatg tgctttcgcc
      961 attgatggga tgatatccag cagcgaactc catgcacctg tgcggatata cgcctgttcc
     1021 gccagacgta atacttccgg atggcgtggc gtaacttcca gcagcttatc cacgccgtgg
     1081 cgtgcagcat ggttttcatt acgggccagt tgcagacgta cacgggtgat ttctaccgga
     1141 atggtgtcat tgccggccag ctctgctgcg cgttccagat gttggttggc gcgtgcttca
     1201 tcaccacgtt gttgcgcggc ttcggcagcc agtagatagt tcaccaccgg ttgttccgcg
     1261 tgatcggcat ttttcgccat cagcttttca acttgctgat aatcgccttc cgccagtttc
     1321 agcagcgcct gttcggtctg cttacgggca cggcgacgct tacgtccgac aaaccaccca
     1381 cgggtgtgtg cgcctgtgcg gaagatccgc cgcagtagcc actcaatggc aaacagcact
     1441 accatcgcca gaatcaaaat aatcgccagg cccgtgacgc tggtttcgat attgtagttg
     1501 tcagtctgga tcagcacgta accctgatga ccggcaatca tcgggccaac cactatcccc
     1561 gcaatcagca aaacaaagag taataacact tttagcatgg ttattctcct tgcggcgcgg
     1621 ctgccggagt atcaccttgc ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     1681 caggtgcagg tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca
     1741 ggttacgcac gcgggtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg
     1801 gaagatccat cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg
     1861 tggtggcatc atcagtatcg tagtaagctc gtacccaggt ggagacgttc tccagcgcct
     1921 ggcgataagt ctcttcctgg tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc
     1981 gaatattttc gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat
     2041 cacgacggcg aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga
     2101 tacgccattc gctgatggaa ctggaaagct cttcaccgtc ggaatccatc ggcgaaccat
     2161 cgctgtcatt atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa
     2221 ggatgatgcc gtcataatcc acctgcgaaa ctgcagaaag gctggcgata tcatcggtaa
     2281 ttgcccgacg aacggtaatc agactcgggt cattcatatc cgccaggctg gcatctgcac
     2341 ttttcagcaa cgctgcagcg gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga
     2401 gtttcaccag aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg
     2461 tggcgacctt ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg
     2521 cctgcttaag ctgtgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct
     2581 cctgggcttt ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat
     2641 tgacggcctg ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga
     2701 tagccaccgc gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag
     2761 gttgtgacgt ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt
     2821 gttccgtcat tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt
     2881 tgtcagcgtt atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca
     2941 aacgctcact gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg
     3001 ggatcagcga ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct
     3061 cgcgggattg ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat
     3121 aacattcaca aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac
     3181 gaccgccatt gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta
     3241 attgtagcaa gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta
     3301 cggtatgtag cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc
     3361 atttacgatc ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg
     3421 caaacaacag atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc
     3481 gacccggaga aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac
     3541 ggctcactaa ctcttctcca gcgggagacg ggcgggtgac aaggatactc atgccggggc
     3601 gtctccgtta tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc
     3661 cagcgaaatc cccatttgtt cggcattttg cggcgcaccg cggcgttcac cgcgaataat
     3721 ctgcgaaccg tccggcgcgc cgaccagcgc acgcagccag atttcaccat caataagctc
     3781 ggcgtagcta ccaattggca cctgacatcc accttcgaga cgggtattca tggcgcgttc
     3841 tgcggtaacg cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg
     3901 cgtatcatca aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat
     3961 ctcgggtggt aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac
     4021 ggcaagaatg atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt
     4081 gccgcgcagg gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg
     4141 taaactggac gtcccgacga tactgcctgc cggtaacgca tccagactgt cataattatt
     4201 ggacacaaag gcatcgcgag gatcttcacg ctcacaaata gtgaccaacc ccagaccttg
     4261 cgggaattca accggcacat ctttcattga atgtacggcg atatcggcgc gattctccag
     4321 gagcgcgact tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt
     4381 atcaagaatc acatcgccgc gcgtcaccat cggtaccagt tcaaccacca ggcccggatg
     4441 gctcgccatc aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg
     4501 tgtggcaatt cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc
     4561 atcctaacat ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa
     4621 accgtaaaat gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga
     4681 atttacagag aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc
     4741 agcaaggtgt taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc
     4801 aggcgcgaaa agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt
     4861 accgttgatg ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt
     4921 acctctatat tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc
     4981 gcgcgcttgc tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt
     5041 tgcactatca ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt
     5101 gcctttacac gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg
     5161 gaatgtcagt acaggaccca ccgaaaggtg agcttccaat tactggtgta tacaccatgg
     5221 gcagcacctc gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc
     5281 aatcctggct cgatagcgaa gagcgccagt tactacaacg taaatgtagt ctgctggaaa
     5341 gctgggccgc ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc
     5401 gtcataatga aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc
     5461 tgcttgacga attttatcgt accgccgtgc gtctcgccgg taagcgtatt ctgtggaata
     5521 tggtgccgtg cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg
     5581 gcgtgctgac gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag
     5641 agtactttgg tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg
     5701 tactgaaaac actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg
     5761 cgaaagatat caaacagcgt ttgcacgacg gcgagattgt atcgtttggt ctcgatccat
     5821 actgcatgat gctggagcgt gttactgaat acctgacggc gattgaagat tttacccgtc
     5881 tggatttagt acgtcgctgc ttctatttaa aagtgtgcga aaagctcagc cgtgaacgcg
     5941 cctgcgtagg ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg
     6001 aagctcgtct ggcaatgctc gataaccgcg ctaactggaa gattgatcag gtgcgtgagg
     6061 cgcacaacga gttgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc
     6121 gtcgcaataa ccttagcgtc tccgccagtc cgcaggatat cggcgtgctg acgcgtaagc
     6181 tgtatgccgc gtttgaagca ttaccaggta aagtgacgct ggtaaacccg cagatttcac
     6241 ccgatctctc ggaaccgaat ctgaccttta tttatgtgcc gccgggccgt gctaaccgtt
     6301 caggttggta tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc
     6361 tggaatataa ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga
     6421 cctcgcgcac ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg
     6481 agatggtcgc cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg
     6541 cgctctacag cccgtgtgag atccgccatc tggcgattat cgttaacctg gaatatgacc
     6601 cgacagcggc gttccgcaat caggtggtgc atttcgattt ccgtaagctg gatgtcttca
     6661 gctttggcga gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt
     6721 ggaacgaagt gcgtacgctg catttcaacg gcgagcaatc gatgatcgaa gccctgaaaa
     6781 ctattctcgg caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt
     6841 atagccagca tctgcgcggc ttaattcgta ctcgcgtgca gcaactggtt tctgagtgta
     6901 ttgaattgcg tctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt
     6961 ctggtcaaac ctgggggttg ttcttcgaac gcctgaatgt atcggtacag aaactggaaa
     7021 acgccatcga gttttatggc gcgatttcgc ataacaaact gcacggcctg tcagtgcagg
     7081 ttgaaaccaa tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca
     7141 tccagttctt tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg
     7201 aaagcaaccg ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg
     7261 acgtcagtcg cttctactcg tcatcgcatg accgttttac ctacggctca agcttcatca
     7321 acttcaacct gccgcagttc tatcagattg tgaaggttga tggtcgtgaa caggtgattc
     7381 cgttccgcac aaaatctatc ggtaacatgc cgcctgccaa tcaggatcac gatacgccgc
     7441 tattacagca gtatttttcg tgatgaacgt gccggaaagc gaggcttatc cggcatgcaa
     7501 tcttagcgga aactgactgt ttcacccgcc tgctgcgtcg ccgcctgttc cagcaaatcc
     7561 cagaaggttt cgccgctgcg atcgcaaatc cactcatcgc cttttaggtc aaaatggtag
     7621 ccgccctgtt tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt
     7681 ttgctgccgt tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg
     7741 ctgtcgccat cccagtcgtc caggcgttct tcaatggtca gccagagttg atcagccagg
     7801 cgatgaaatt cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag
     7861 cggcatgggg tcagggcttc aaagtttgca cctctgcggc tgcgttccgg cacgattcat
     7921 ccgtcaccgg aataatgatg tctcagtgta gcgaaagatt tgtctcttca ttagggcgca
     7981 gttacaccac gcctttccct gtttctggta aacattatga tcaggtttac cgagcgagca
     8041 tcctcacgct gacggaacta aaaaagccaa caaacaaact acattgcgat agtgcataaa
     8101 gccatcctgg cgcgaggtgc cgatcacgaa actaccagca aaacataaat ccccacgagt
     8161 aagcgttata ctcgcagcat ttcctcactt ttcagacttc ataaagagtc gctaaacgct
     8221 tgcttttacg tcttctcctg cgatgataga aagcagaaag cgatgaactt tacaggcaat
     8281 ccataatgaa aaacgtgttt aaggcactca ctgtattact tactctcttc agcctgacgg
     8341 gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga taaaaacgca ccgccgccga
     8401 ccaaaccggt agagacgcaa acgcaatcca cggtgccgga taaaaacgat cgcgccactg
     8461 gcgatggtcc atcccaggtg aattactaaa agtcagtttc tgtacccgcg tgattggagt
     8521 aaatgatgca gttctcgaaa atgcatggcc ttggcaacga ttttatggtc gtcgacgcgg
     8581 taacgcagaa tgtctttttt tcaccggagc tgattcgtcg cctggctgat cggcacctgg
     8641 gggtagggtt tgaccaactg ctggtggttg agccgccgta tgatcctgaa ctggattttc
     8701 actatcgcat tttcaatgct gatggcagtg aagtggcgca gtgcggcaat ggtgcgcgct
     8761 gctttgcccg ttttgtgcgt ctgaaagggc tgaccaataa gcgcgatatc cgcgtcagca
     8821 ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga tctggtccgc gtaaatatgg
     8881 gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc taacaaagcg gaaaagacct
     8941 atattatgcg cgccgccgag cagacaatct tatgcggcgt ggtgtcgatg ggaaatccgc
     9001 attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt agaaacgctt ggtcctgttc
     9061 tggaaagcca cgagcgtttt ccggagcgag ccaatatcgg ttttatgcaa gtggttaagc
     9121 gcgagcatat tcgtttacgc gtttatgagc gtggggcagg agaaacccag gcctgcggca
     9181 gcggcgcatg tgcggcggtt gcagtaggga tccagcaagg tttgctggcc gaagaagtac
     9241 gcgtggaact ccccggcggt cgtcttgata tcgcctggaa aggtccgggt cacccgttat
     9301 atatgactgg cccggcggta catgtctacg acggatttat tcatctatga agcaaccagg
     9361 gaaagaactg caggaaacac tcacggagct tgatgaccgg gcggttgtcg attatctgat
     9421 taaaaatcct gagtttttta tccgcaatgc gcgcgcagta gaagcgatac gtgtgccgca
     9481 tccggtacgc ggcaccgttt cgttggtcga gtggcacatg gcccgcgcac gtaatcatat
     9541 tcatgttctg gaagagaaca tggcgttgtt gatggaacag gctatcgcca acgaaggcct
     9601 gttttatcgc ttactctacc tgcagcgcag tctcaccgcc gccagcagtc tcgacgatat
     9661 gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg gctggtgcga gtctgcgcct
     9721 gtttccggat cgctggcgct taggtgcgcc gtcgaaccac actcatctgg cattaagccg
     9781 tcagtctttc gaaccgctgc gtattcagcg tttggggcag gaacagcact atcttggacc
     9841 gcttaacgga cctgagctgc tggtggtgct acctgaagcg aaagcagtgg gatcggtggc
     9901 gatgtcgatg ctgggaagcg atgctgattt gggtgttgtg ctgtttacca gtcgcgatgc
     9961 cagtcactat caacaagggc aaggaacgca gttacttcat gaaattgcgc tgatgttgcc
    10021 ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt tacacaccga tgtagaacgc
    10081 tacctacgtt atctgagcgt ggagcgccag cttagcccga taaccctgct taactaccag
    10141 cgtcagcttg aggcgatcat caattttgcc agcgaaaacg gcctgcaaag ctggcagcaa
    10201 tgtgatgtga cgatggtgcg caattttgct gtacgcagtc gccgtaaagg gctgggagca
    10261 gcaagtctgg cgttacggct ttctgcgcta cgtagctttt ttgactggct ggtcagccag
    10321 aacgaactca aagctaaccc ggcgaaaggt gtttcggcac cgaaagcgcc gcgtcatctg
    10381 ccgaaaaaca tcgacgtcga cgatatgaat cggctgctgg atattgatat caatgatccc
    10441 ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg gcgcgggtct gcgtctttct
    10501 gagctggtgg ggctggatat taaacacctc gacctggagt ctggtgaagt gtgggttatg
    10561 gggaaaggca gcaaagagcg ccgcctgccg attggtcgca acgctgtggc gtggattgag
    10621 cactggcttg atttgcgcga cctgtttggt agcgaagacg acgcgctttt tctgtcgaaa
    10681 ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct ttgccgaatg gggcataaaa
    10741 caagggctga ataatcacgt tcacccacat aaattacgtc actcgttcgc tacgcatatg
    10801 ctggagtcga gcggcgatct tcgtggtgtg caggagctac tgggtcatgc caacctctcc
    10861 accacgcaaa tctatactca tcttgatttt caacaccttg cctcggtgta cgatgcggcg
    10921 catccacgcg ccaaacgggg gaaataatgc gtttttaccg gcctttgggg cgcatctcgg
    10981 cgctcacctt tgacctggat gatacccttt acgataaccg tccggtgatt ttgcgcaccg
    11041 agcgagaggc gcttaccttt gtgcaaaatt atcatccggc gctgcgcagc ttccagaatg
    11101 aagatctgca acgcctgcgc caggcggtac gggaagcgga acccgagatt tatcacgacg
    11161 tgacgcgctg gcgttttcgt tcgattgaac aagcgatgct cgacgccggg ctgagtgccg
    11221 aagaagccag tgcaggcgca cacgcagcaa tgatcaactt tgccaaatgg cgcagccgaa
    11281 tcgacgtccc gcagcaaact cacgacacct taaaacagct ggcgaagaaa tggccgctgg
    11341 tggcgatcac caacggtaac gcccagccgg aactgtttgg tttgggggat tattttgagt
    11401 ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt cagcgatatg tactttctgg
    11461 ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca tgttggggac gatctcacca
    11521 ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg ttggatcaga ccggaaaatg
    11581 gcgatctgat gcaaacctgg gacagccgtt tactgccgca tctggaaatt tcccggttgg
    11641 catctctgac ctcgctgata taatcagcaa atctgtatat atacccagct ttttggcgga
    11701 gggcgttgcg cttctccgcc caacctattt ttacgcggcg gtgccaatgg acgtttctta
    11761 cctgctcgac agccttaatg acaaacagcg cgaagcggtg gccgcgccac gcagcaacct
    11821 tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta ctggtgcatc gtatcgcctg
    11881 gctgatgagc gtggaaaact gctcgccata ctcgattatg gcggtgacgt ttaccaacaa
    11941 agcggcggcg gagatgcgtc atcgtatcgg gcaactgatg ggcaccagcc agggcggcat
    12001 gtgggtcggc accttccacg ggctggcgca ccgcctgctg cgtgcgcacc atatggacgc
    12061 caatctgccg caggatttcc agatcctcga tagcgaggac cagctgcgcc tgcttaagcg
    12121 tctgatcaaa gccatgaacc tcgacgagaa gcagtggccg ccgcgccagg caatgtggta
    12181 catcaacagc cagaaagatg aaggcctgcg tccgcatcat attcaaagct acggtaatcc
    12241 ggcggagcag acctggcaga aggtgtatca ggcgtatcag gaagcgtgtg accgcgcggg
    12301 cctggtggac ttcgccgagc tgctgctgcg cgctcacgaa ctgtggctta acaagccgca
    12361 tatcctacaa cactaccgcg aacgttttac caatatcctg gtggacgaat tccaggatac
    12421 caacaacatt cagtacgcgt ggatccgcct gctggcgggc gacaccggca aagtgatgat
    12481 cgtcggtgat gacgaccagt caatctacgg ctggcgcggg gcgcaggtgg agaatattca
    12541 gcgtttcctt aatgatttcc ccggtgccga aactattcgt ctggagcaaa actaccgctc
    12601 taccagcaat attctgagcg ccgctaacgc cctgattgaa aacaataacg ggcgtctggg
    12661 taaaaaactg tggaccgatg gcgcggacgg tgagcctatt tccctctatt gcgcttttaa
    12721 cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa acctggcagg acaacggcgg
    12781 ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc cagtcgcgtg tactggaaga
    12841 agcactgttg caggccagta tgccgtaccg tatttacggc gggatgcgct tcttcgaacg
    12901 ccaggaaatc aaagacgcgc tctcgtatct gcgcctgatt gccaaccgta acgacgacgc
    12961 ggcctttgag cgtgtggtga atacgccaac gcggggtatt ggtgaccgga cgctggacgt
    13021 ggtacgtcag acatcgcgcg atcgccagtt aacactctgg caggcatgtc gtgaactgtt
    13081 gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta cagcgattta tggaattaat
    13141 cgatgcctta gcgcaagaaa ctgccgatat gccgctgcat gtacagactg accgggtaat
    13201 taaagactcc ggcctgcgca ccatgtacga gcaggagaag ggcgaaaaag gccagacgcg
    13261 tatcgaaaac ttagaggaac tggtgacggc aacgcgccag ttcagctaca acgaagaaga
    13321 tgaagattta atgccgctgc aggcattcct ctcccatgcg gcgctggaag cgggcgaggg
    13381 gcaggcggat acctggcagg atgcagtgca gttgatgacg ctgcactcgg cgaaaggcct
    13441 ggagttcccg caggtgttta ttgtcggtat ggaagagggt atgttcccaa gccagatgtc
    13501 gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg gcctacgttg gtgtaacccg
    13561 cgcgatgcag aaactgacgc tgacctacgc ggaaacccgc cgtctgtatg gtaaagaggt
    13621 ttaccatcgc ccgtcgcgct ttatcggcga gctgccggaa gagtgtgtgg aagaggtgcg
    13681 cctgcgcgct acggtaagtc gtccggtcag ccatcaacgg atggggacgc cgatggtcga
    13741 gaacgacagc ggctacaagc tcggtcagcg tgtacgccac gctaagtttg gtgaaggcac
    13801 catcgtcaat atggaaggca gcggcgagca tagccgtttg caggtggcat tccagggcca
    13861 gggtattaaa tggctggtgg cggcatacgc ccggctggag tcggtgtaac gttgccggat
    13921 gcggtgctgc gcaccttatt tggcctaaaa aatcattcat attcaataaa ttgcaacgtc
    13981 atgtaggccg gatagggcgt ttacgccgca tccggcatct gcgccatctt cagtatctga
    14041 cacaaaacta tcgttttaac ctttccgctc tacggaaatc atggtgacaa aaggatagcg
    14101 ttgccacggg attgccccgc ctttcatata catatgtgaa atcgtgccat caagataaag
    14161 cagctgctca acgttcagtt tcgctttggc ataacaggca aaatcataaa aatttgtcgc
    14221 ctgctggctc aacaaaaaca cggcattccc atgtttatta atcccaacac cgttacgaat
    14281 tttacgtgag gcgacgttgg gatgaatacg cggattaatt acactgtttt ccatcaacat
    14341 tggccctgac tgcaccgcaa actgaatctc tttactggtt ttgaaggcat ccagacgaac
    14401 gatgccgact ttatctcccg cgacataaaa cacgccgcca ggacggataa agaaattccc
    14461 ttcacctgaa gcgagattta acgccacctt ctgctgaccg ttttcgatgt acaaaccgag
    14521 cggcgcatag ctttcatcat agatgccgcc gttcatcgcc atctgcacct gaccctgact
    14581 attcatatcc gccagcagag catgtaaagt tccccacgct tcgccattgg ctttttgcca
    14641 gtacattttc acccgctctg tttgaggatt aacggtatac gcctgtacgg tcagcgtcgg
    14701 atctgagagt gcgcaatcat cagcggcaac agcaaacaag ggaagtaagg tgagggcgag
    14761 aaaaatccgt ttgagattca aggtgatcat tcctttacca atgagtagcc gatgcgccat
    14821 tataggtcct ggatgttgga tttttttatc ctgttagcga ccttgacgag taccaaaaag
    14881 cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg ttgacggcaa aattttgctg
    14941 gcgtaacatg cgcgcacgat cactctaaga ggacattcgc cttggacaca cccagtagat
    15001 actggctcac tatcctgtca tccaggatca actcctaagg ctatcccttt ttgctgatag
    15061 ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc tgagtcaggc tgtttaatgg
    15121 tcagaaaccc aatttgtttc tgtgtgccca ccgaactgtc cgatatttta agcattggga
    15181 gtcccggtca tgctgagcgc atttcaactg gaaaataacc gactgacccg gctggaagtc
    15241 gaagagtcac aaccccttgt aaatgcagta tggattgatc ttgtcgaacc ggacgacgac
    15301 gagcgactgc gcgtacaatc tgaacttggc cagagcctgg caacccgccc ggaactggaa
    15361 gacatcgaag catcggcacg tttctttgaa gacgacgacg gcctgcatat tcactccttc
    15421 ttcttctttg aagatgcgga agatcacgcc ggtaactcca ctgtggcatt taccatccgt
    15481 gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg cttttcgtct gtatcgtatg
    15541 cgtgcccgta gccagtcgat ggtagacggt aacgcctacg agttgctgct ggatctattc
    15601 gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata tctatagcga cctggagcag
    15661 ttgagccggg tgattatgga agggcatcag ggcgatgagt acgacgaggc gctctccacg
    15721 ctggcggaac tggaagatat cggctggaaa gttcgcctgt gtctgatgga tacccagcgc
    15781 gcgctcaact tcctggtgcg taaagcgcgt ttaccgggtg ggcaactgga gcaggcgcgt
    15841 gaaatcctgc gagatatcga atccctgctg ccgcataacg aatccctgtt ccagaaggtg
    15901 aacttcctga tgcaggcggc aatgggtttt atcaacatcg agcagaaccg catcatcaaa
    15961 atcttctcgg tggtatccgt ggtattcctg ccgccgacgc tcgttgcttc cagctatggg
    16021 atgaactttg agtttatgcc agaactgaag tggagtttcg gctacccagg cgcgattatc
    16081 tttatgatcc tcgcgggcct ggcaccgtat
//
LOCUS       DAPPNJ010000102        16055 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000102 DAPPNJ010000000
VERSION     DAPPNJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.102"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..998
                     /gene="phoE"
                     /locus_tag="RPM73_002701"
     CDS             <1..998
                     /gene="phoE"
                     /locus_tag="RPM73_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDX6521046.1"
                     /translation="AAEIYNKDGNKLDVYGKVKAMHYMSDNASKDGDQSYIRFGFKGE
                     TQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDV
                     EAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRD
                     VKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYD
                     ANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEG
                     IGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
     gene            complement(1037..1438)
                     /gene="crl"
                     /locus_tag="RPM73_002702"
     CDS             complement(1037..1438)
                     /gene="crl"
                     /locus_tag="RPM73_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDX6521047.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1496..2740)
                     /gene="frsA"
                     /locus_tag="RPM73_002703"
     CDS             complement(1496..2740)
                     /gene="frsA"
                     /locus_tag="RPM73_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDX6521048.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2832..3290)
                     /gene="gpt"
                     /locus_tag="RPM73_002704"
     CDS             complement(2832..3290)
                     /gene="gpt"
                     /locus_tag="RPM73_002704"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDX6521049.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3551..5008
                     /gene="pepD"
                     /locus_tag="RPM73_002705"
     CDS             3551..5008
                     /gene="pepD"
                     /locus_tag="RPM73_002705"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDX6521050.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5065..5679)
                     /locus_tag="RPM73_002706"
     CDS             complement(5065..5679)
                     /locus_tag="RPM73_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDX6521051.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5676..6815)
                     /locus_tag="RPM73_002707"
     CDS             complement(5676..6815)
                     /locus_tag="RPM73_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDX6521052.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7061..7513)
                     /locus_tag="RPM73_002708"
     CDS             complement(7061..7513)
                     /locus_tag="RPM73_002708"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX6521053.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7510..8565)
                     /gene="dinB"
                     /locus_tag="RPM73_002709"
     CDS             complement(7510..8565)
                     /gene="dinB"
                     /locus_tag="RPM73_002709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDX6521054.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8636..9559)
                     /gene="lafU"
                     /locus_tag="RPM73_002710"
     CDS             complement(8636..9559)
                     /gene="lafU"
                     /locus_tag="RPM73_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDX6521055.1"
                     /translation="MRKTGNRRDRGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            complement(9562..10425)
                     /gene="motA"
                     /locus_tag="RPM73_002711"
     CDS             complement(9562..10425)
                     /gene="motA"
                     /locus_tag="RPM73_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDX6521056.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10438..11154)
                     /locus_tag="RPM73_002712"
     CDS             complement(10438..11154)
                     /locus_tag="RPM73_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDX6521057.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGILLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11174..11641)
                     /locus_tag="RPM73_002713"
     CDS             complement(11174..11641)
                     /locus_tag="RPM73_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDX6521058.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11649..12710)
                     /locus_tag="RPM73_002714"
     CDS             complement(11649..12710)
                     /locus_tag="RPM73_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDX6521059.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVRDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12707..13018)
                     /locus_tag="RPM73_002715"
     CDS             complement(12707..13018)
                     /locus_tag="RPM73_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDX6521060.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLCAVKARLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13023..13415)
                     /gene="fliS"
                     /locus_tag="RPM73_002716"
     CDS             complement(13023..13415)
                     /gene="fliS"
                     /locus_tag="RPM73_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDX6521061.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13438..14754)
                     /gene="fliD"
                     /locus_tag="RPM73_002717"
     CDS             complement(13438..14754)
                     /gene="fliD"
                     /locus_tag="RPM73_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDX6521062.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(14961..15875)
                     /gene="lafA"
                     /locus_tag="RPM73_002718"
     CDS             complement(14961..15875)
                     /gene="lafA"
                     /locus_tag="RPM73_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HDX6521063.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         3733 a         4285 c         4023 g         4014 t
ORIGIN      
        1 aggctgcaga aatatataat aaagacggta ataaactgga tgtctatggc aaagttaaag
       61 ccatgcatta tatgagtgat aacgccagta aagatggcga ccagagttat atccgttttg
      121 gttttaaagg tgaaacacaa attaacgatc aactgactgg ttatggtcgg tgggaagcgg
      181 agtttgccgg aaataaagcg gagagtgata ccgcacagca aaaaacgcgt ctcgcttttg
      241 ccgggttgaa atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt
      301 atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgattcc tcggcgcaga
      361 ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct
      421 tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg
      481 acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca
      541 gcgatttcgc cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc
      601 aaagccgtgg cacaggcaag cgtgcagaag catgggctac aggtctgaaa tacgatgcca
      661 ataatattta tctggcaact ttttattctg aaacacgcaa aatgacgcca ataactggcg
      721 gctttgccaa taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc
      781 tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgaaggt atcggtgatg
      841 aagatctggt caattatatc gatgtcgggg ctacatatta tttcaacaaa aatatgtcag
      901 cgtttgttga ttataaaatc aaccaactgg atagcgataa caaattgaat attaataatg
      961 atgatattgt cgcggttggc atgacctatc agttttaata gatattgccg gatgtgatgc
     1021 atccggcaca tttcactcac gccgttaact tcaccggctc gtcacgaaaa tcatccgccg
     1081 gttccagctt cagattcagc gtcgtcagca gttcacgcag cttctcgtga aactcacgca
     1141 gggtgtgctc cagtcgttca accacttcag tatcttttac cggaacactc ttccagtcgc
     1201 ctgctttatc gaacagacca aactggtagc tgtaggtaaa acgggattcc tgcgcttcca
     1261 gctccatcca ccagccccag aactcacgca cttccggtgc cggtttcacg ttgacgcata
     1321 cagccagaca atcgaaaaag aatcgattat cttcgcactt accttcacga atatacgggc
     1381 ctagtgcggt aaattttttg atcaatctgc tcttcgggtg tccactcggt aacgtcattg
     1441 caatctcctg ttgtgatgca actgttttac caaattggta aaatttagca aatttttaac
     1501 acaaacgttt ttcgatccag tcggtgattt cctgaagacc tttgtcaaaa ttccgataca
     1561 ccgggttaaa tgggatctct aataatttac cgtcagcaga tgatgaggtg attaagcgtg
     1621 agtcctcttc cgggctgaac ggatcgttct tccagtagcc tgataacatt ggcgttgggc
     1681 agcgacgtcc cagcaatcct tgcactttta atgaatagcg attcaactcc acgcgcaacg
     1741 cttcgtcgga agcatcatgc atccccaaac gactcgccag aacgtcaaga tacatttccg
     1801 gcacctgttg ctggcactta aaatcactca acagggtatg aactaccgga ccaagacagg
     1861 caaccgcttt cagacgcggc gattcaaggt atgccagacg cacggcaacg ttagcgccga
     1921 aacggaaacc aaaggccgcg acgcgagtgt gatccaccca cggtacgtta ggcagcgcct
     1981 ttaagacgtg ctgatgcaac aggctggagt cctgggtgag cttccatttt gaagaaaagc
     2041 ccaccgacgg catatcaata gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat
     2101 acaggctgta atagtccgtc tgcatcgcat ccagaccacc acacattaat actgtcggga
     2161 acgggccatc gcctttcggc atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg
     2221 taaactccat ctgccgcatc gtgcccggca gacgctgggc ggcctcttca taggcgcggt
     2281 ttgataaagc ctgcgcttgc tcggccaggt catctccttt cagatgagga taggcggcaa
     2341 tgttgtacaa cgtagccgca tgcagccagt gtcgaccact gagttgcgga tcttcttccg
     2401 cacaggcttt ttgttgccac accatcgcct gggtggccca ctcataaatc cagttgccac
     2461 cacggtagcc aatcacggta tcgtataaat catcatcggt acgttcagca tcgctcatca
     2521 caatacgtgc ctggacgtcg aggatttcac gtgggtcaat gccgcgccag atccacatca
     2581 gacggttaat catgcgatac cagtgaggga tggttttacc atcaagagcc gactgcacag
     2641 gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc
     2701 ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac
     2761 ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg
     2821 cgttgaaaag attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc
     2881 tgttcgatcc aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca
     2941 gccggttttg cgaagatagt cacaaaatgt gcttttggat acatttcacg aatcgcaacc
     3001 gcagtaccac cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct
     3061 gcgcgtttta gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg
     3121 gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac cagaccgcca
     3181 cggcttacgg caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta
     3241 cgtgcatgga tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc
     3301 ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta
     3361 tagagatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg
     3421 cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg
     3481 ttgacaacat tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag
     3541 gagacttaac gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc
     3601 caaaatctgt tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt
     3661 tggttgggca aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat
     3721 tcgtaaacct gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct
     3781 cgatatggtg ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca
     3841 gccttatatt gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg
     3901 cattggtatg gcctctgcac tggcagttct ggctgacgaa aacgtggttc acggcccgct
     3961 ggaagtgctg ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag
     4021 caactggttg caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta
     4081 catgggttgt gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt
     4141 tccagctggt tttgaaacct tcaagttaac cttaaaaggt ctgaaagggg gtcactccgg
     4201 cggggaaatc cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg
     4261 tcatgcagaa gaactggatc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc
     4321 catcccgcgt gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgtcctgaa
     4381 atctctggtg aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa
     4441 tctggccttg ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg
     4501 cgataccttt attcgtctgc tgaacgccac cccgaacggt gtgatccgca actcagacgt
     4561 ggcaaaaggt gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgataataa
     4621 cgtagaaatt cactgtctga tccgttcact gatcgacagc ggtaaagact acgtcgtgag
     4681 catgctggat tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc
     4741 tggctggcag ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg
     4801 tctgttcaac aaaacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt
     4861 caaaaaaccg tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca
     4921 ctctccggat gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga
     4981 actgctgaaa gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc
     5041 gccttccggc agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaatgtccg
     5101 tctcgggttg ccacgttcaa tctggtgatg gaacattcgc cgctgcgatt tcagcaccgc
     5161 gctattttcc tgttgctgct gctccagctt ccaggcaatc agcaatcgtg ccagccgctt
     5221 gttagcatgc tgactacgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt
     5281 ggcgcgtacc gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcaa
     5341 cgtctcataa cggattgcat ccgattgttc ctgctcatca gcggtaaaac gcccaatgcc
     5401 cagaaaccag tttttgcgcc catgatgagg ccgatacgga ctcggacaaa tccactgaat
     5461 agtgccacac cagctttcgc ttaatgccca tgcgttatcg ccatccagag aaatcagcgc
     5521 cgaacgcagc gtgtcagagt agcggcccgt ttctgtttcc aacaccgtta ccgcgacgtc
     5581 ttgtcgggca gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc
     5641 ttccggcccc tgcgctgagg agagttgtag caagatcatc cttttttccc tccactgttt
     5701 ttgagcgtta gcaccggacg cagccgcgcg acaggaataa ttaaaccggc ctgcaccaga
     5761 cattgcacca cgctttcagc cgatttatag gcttgtggcg cttcttcaaa gatgagttgt
     5821 ttatcgcgac aaattacccg actgccaagt tcagtccgcg agagttgcgt caccgtgtat
     5881 ttcgctgcca gacgcccttt acactcggtg cgcccccatt tacgccctgc cccatgcgcc
     5941 agcgagtgga gcgttttttc gttcgcaaca ggctgtacaa tccaggagta atcaccacgt
     6001 gaaccgggaa taatcaccag accgttgtca tccggcgtgg cacctttacg atgcaaccag
     6061 ccctgttgat cgccgatttg acacgcgcta acaaagttat gcgccacatc cagaaccgga
     6121 cttcccgtgg ccttaacttg ttgcattatg cgcaaagcga tcatctggcg attaatacac
     6181 gcaaacgcca gcgcatcgtc atgttccgca atataccgta gcgcgtcgtc actgccttga
     6241 ggcaaaccat gatgcgagaa cgaggcaata tgccgctgta aaatagactg ccccaccccc
     6301 cgcgagccgc tatgaaccaa cagttgcaga tgttgtgcat ccagacctgc cagtgcaaac
     6361 agttcagcgt tgatgatttg atcaacttgt tgcagttcag cgaaatgatt gccgccaccg
     6421 atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg ctgacggcag gttttcctcc
     6481 agccagcttt cttcagcaac gtcatccaga tctgataatc gcttttcaaa cttatcggcg
     6541 ttgtatttgc gagcgagaat atctgtttgc catagcgcca taccgcagcc gatatcattg
     6601 ccgaccagtg ccggataaaa acgaccaaca gagaagaacg ctgcgccaat cggatagccg
     6661 cgtccgggat gtaaatctgg catccccact acgcgctgca tgttgggtaa atttgccgtg
     6721 gtgtgtaatt gttggatcgc taaactctcg atccacaggt catcagatgc gatggtaaat
     6781 accgcatcgg ataagggacg aatataattg cccatatacc attccgtaaa tagactcgtc
     6841 atacttcaag ttgcatgtgt tgcgacttga attattgaga gtaacaatta aaaaatcagg
     6901 aaatggcaga cgaatccagg gcaagaaaaa aagaattaat cctggaaagg tctgcaaaga
     6961 gtcattgata aaggatttgt catgatttac ctccttgcag tttgtggaat aagaaaacgc
     7021 gcacatacta ggcttgcgtt attttcattg caagctggat ttaatgttgc tgtttataac
     7081 gcatataaaa attaataaac cactctcccc gacattcaac gcgttgctgc ttaactgtct
     7141 gaaaaccata acgttcaaaa aagggttttg cggttatgct tgcgtccacc gtaagttcgg
     7201 attcagactt aatcaaaggt tttaacaaag cgctggcaac cccacggcgg gtgtattcag
     7261 ggtcaacaaa taacatatcg atatagtgtt caacgcaggt aataaagccg accagttttg
     7321 cattaatgac tgcaacccgc acttgtgatt tcgcgagttt ctccttccag cgagattcgt
     7381 caatctgcgc ccaggcggca atttgttgtg gtgaataatg ctgactggca gtcatcgtaa
     7441 ccgctctaat gaaaatagcg catagttgct gaaaatcgcc aggctgatag tctcttattt
     7501 gtatgttatt cataatccca gcaccagttg tctttccatt tgcgggtcaa gcaacgtcac
     7561 atgcagcccc accagtcgca caccgcgccc gccgcggcgt tcatcccagg ttttacgcgc
     7621 ggtggcgatt aagtcagctt tattcagccg cggccagacg tgctcctgag tggtttgctg
     7681 aaaatcgtcg aactttaatt tcaccccctg gcgagcaatc agtaaatcag gtttcacctt
     7741 tgccagacgg cgttcaagtt ccggatacag ccgctcgata atcgcttcac attcagacca
     7801 gtggtggata tcttccgcca tcgtgcgttc cacgccgacg gattttcgca accgttcgct
     7861 gttaacgtcg cgctcgtcaa tcccctgact acgctcccac aaaatgcggc caaatttgcc
     7921 aaagcgttta agcagcatca ccagatcaca cttttgtaca tcaccgcagg ttcgtagccc
     7981 catcgcttcc agttttgccg ccgagacttt gccgacgccg gggatttttg ccagtggtaa
     8041 ggtttgtaaa aatgccggaa cttctgccgg cgtaatcaca aactgaccgt tgggtttatt
     8101 catgtcggag gcgattttgg cgagaaactt tactggtgcc acgcccgcag atgccgtcag
     8161 ttgcagctcg ttgaaaatcg tctggcggat ttcctgggcg atgagggtcg cagaaccgtg
     8221 gcaatggacg ctatcggtga catcgagata agcctcatcc agtgacaacg gttcaatgcg
     8281 cgaggtgtag cgcgagaaga tttcacggat atgatttgag gcttctttgt aggcgtcaaa
     8341 gcgccccgga agcaaggtga gatgtgggca taatttgagc gccatcccag taggcatagc
     8401 gctacgtacg ccaaatttac gcgcgggata attggcggtg ctgatcaccc cccgacgttc
     8461 gcggctgccg ccaatagcaa tagggatatc gcgcagggcg ggattgtcgc gcatctccac
     8521 tgcggcgaaa aagcagtcca tatccacatg aatgatttta cgcattgctc acctctcaat
     8581 actggtaaag tatacagtga tttcagggtt tgagaaatgc gtaaagattc agcatttagc
     8641 gcattcgctg cgaaagcacc tgctgcttat ccagcttttg caccagcggc tgcaccactt
     8701 tatccccatg ctgaccaaag tattgataca gcgtatcgga cgcacttttg gtcagcacca
     8761 taatctcaat acgccgattg cccgcgcttt gcggattttt ggcatccagc agcatctggt
     8821 ccgccattgc gcttacctgc atcactttat cttccggcat tccggcctct tccagcaccc
     8881 gacgagctga aagcgcgcga tcgcccgaaa ggttccagtt gttgtagata ttgtttttgt
     8941 aggccatcgc atcggtatgc ccggtaataa taattttgtt atcgagcgag tcgaacactg
     9001 gcgcgagctc caccagcaaa gttttaaaga acggcataat ctgcgcgctg ccgcgttcga
     9061 acatattgcg gttctggtca tctttaatca gcacgcgcaa tccctgggga acaatctcca
     9121 tctccagatt cgcttccata tgcgcatcac gggcaatggt gttgatactg gttgccagct
     9181 cgccaagctc ggtggcggat ttcttcttca gcagcgctgt tttctcgttg acgtctcgcg
     9241 cttttttctc cgtctcttcc ggcaccgcca cggtggcagg ttttggcgga tgagacgggc
     9301 tgattttatt taacggcgac aatccgccgc cactgaaaat cgactgcccg tgcagcgcgg
     9361 cgataatgct ttcacgctcg gatttactga cgctgttgac aatccacagc gtcataaaca
     9421 gcgccatcat tgccaaagta aagtcggcga acgccacctt ccacgccccg ccgtggtgcc
     9481 cggcatgatt ctttttgatc tgccgccgga cgatggtggt ttttgcccca cgatcgcggc
     9541 ggttacccgt ctttctcatg cttattcttg ctccagcatc gcattcaccc acgcgtcgag
     9601 gttggcgaag gtcggtttgg acgccagatg cagtagctta cgcccggcgt ccaccgccag
     9661 cagcgtcggt ttgccgcctg cctgcgccac cagcacggta cgcacgcatt ccagcaaaga
     9721 atgttctgcc cgcgcctgtt gttccatggc gttggcgagt gggtccatca ggcagtagca
     9781 gataaagacg cctaaaaatg tccccaccag cgctgccgcc actttcaggc caatcagggc
     9841 gatagagccg tcaatcgact gcatggtgat gatgatgccc aacaccgccg cacaaatgcc
     9901 aaaccccggc atcgcttcgg cggtacgttg cagcgagcgc gacggcgtga gcagcgactc
     9961 ttccgcagtg tccagttcct gatcgagaat gccttccagt tcatgggcgt cgattttacc
    10021 catcgccatc aggcgaaagt tatcggcaat aaaggtcacc agccgcttct gggttaacac
    10081 cagcgggtat ttctggaaaa tggtgctctc ttccggttgc tcaatatgtt gatccagcat
    10141 gcgcagacca ccgttttgca ccatttcgag taactcatac aggcacatta atagctggcg
    10201 ctgaaactcc ggcccgagct gttttttact gattacgcct ttaatttgat gggcaatctc
    10261 tttcagcaca tgtttcgggt taccaataat catcgccccg aaaccggcac ccaaaataat
    10321 gatcatttcc gccggttgcc agatagccac taattgcccg cccgcttcaa aataaccgcc
    10381 aaatacgcag ccgagcacca ccagtaaacc taatattttt tgcatgattt cttcacttta
    10441 attcggatag acaaaatcac gaattttctg cgcgatctgt ttattcagtt gacaaattcg
    10501 ggcctcggtt agccctaata ccagcgcaat ctctttgaga ttcatctcat gctggtaata
    10561 catggagagg atcagttgct ctttttcgct taattgtgaa agcgcctgtt gcagcatttg
    10621 ctgggtgaca aactgatctt ccagctccct gccagcaagc agaatgccag gcccttcgcc
    10681 gttcagcagt tcgtccaggc tggcgagcgt ttccgcccct tccagttgct gatattcgag
    10741 ataatcttca tgtgagatcc cttccacggc cagttctgac cactccggtt cgtgccccag
    10801 ttttttgcgc gtttcgcgaa tcagatcttt catctgatga tatttctgtc gcagctggcg
    10861 cggtcgccag tcgagagcgc gcagctcatc caggatcgcg ccgcgaatac ggtgcacggc
    10921 atagcccgca aaaccttcgt ccggcagccc gtagcggcgg atggcattaa gcagccccat
    10981 taacgccgtt tgttccatat cctgacgatc aataatgcaa ttacactgcg gggcaagttg
    11041 ccgcaccact ttacgcacca acggtaaata ggcctgcaag tagtggctct cctgctgcgg
    11101 ggttaatacc ggcgcggcag caaattcttc tgactcgaat acatcctgta gcatgtttat
    11161 atccgcctgg tgattactgg aagaccactt ttttcaccag caaatcctta tacggtgcgc
    11221 tcattttgcg acgctgaaga tctttcttta acgcatccga aatgagtttg cggatggcgg
    11281 atattttcat gccacgcaaa tcttcaaatt tcatttccgt caggcattca accgcaatac
    11341 tttgatacag cggctcctga tgttttattt tttccgcttc tttgtcactg gcgaccacca
    11401 tcactaattc cgccgacaaa taatggtccg cgccgttatt gtcatgcagc gtgacaatgg
    11461 tttccggcaa cgtcacaaac acgcttttgc tctcatccag ggtttccacc gtttccgtct
    11521 gcgcggcggc agtcggtttt ccttttccgg cataatgatg ccagacaccc catccagctc
    11581 cggcccccac caccaacgcc agaacggcgc tgaccacgcc cgccaccacg attttcttca
    11641 tattgccttt acactttgat caaaacggat tcgttctgtt gaaattgagg cgcatcatca
    11701 agctgtaagg cagcactcac ctcttcctgc tgatggcccg actgttgttg ttgctgcgac
    11761 tgcccttcgg aggaaacctg tacgttcacc tccataaaat tttgcgccgt cagatgctgg
    11821 cggagatcgt cgctaaactg ctgtaacgcg cggcaaactt cactttggtt cgcgccgatg
    11881 tgcaccatca gtttgccgtt gtcgagttgt acggcgatct cgagcttgcc aagcgacggt
    11941 ggatcgagac ggatggtgga gatctgctgt tgtttgttga gctgaaagtg gatctgatct
    12001 ttcaacagcg tcgtcagttt ttcgcccagt tcatccatcg aaagcgtggc tggtttggcg
    12061 acaatctcct gatgatcgcg gaccagctcc ggcgtcttaa cctgcaacac cgcacgcgca
    12121 ataactggtt cgctgtccgg cactttttcc gttttacgct cgacctgcgg gcgcgcggtc
    12181 acacgggtta gttcaggttt cgggcgagct ggctgtgttg agacgcgcgg ctgcgtcggc
    12241 gcaatggcat gtaaatcttc actggcgtaa gtcgctaatc tggcctgctg ttcgggcttc
    12301 gcctgcggca actgactgat cagttcctgc aactgcggcg gcaacggcgt gagttcctgc
    12361 tgttgcgtcg ggcgttgcgg cgcgttttgc accaccgctt tcgtcaattg ctggataacc
    12421 ggcgatgttg cgacgtttcg cggctgcggc gtgtcctgat gcggcgcggc aggttgcgga
    12481 agtaacagcg ccattagcgc ctgcatcgcc accggatcgt tctcctgcgg gcgcgtcgct
    12541 tgttgctgac tggttttagc gctatgaacg cgctccgcca ccgccgcgac cgccattttc
    12601 ggcagggtaa acgcgggcgc gttttcaccg ctcaccggcg gtaaaatatc cgccttcaac
    12661 gaagcggttt ccgcaagcgt tccgagcgtg gcaagcaatg ccggattcat gacttctccg
    12721 aaagtaaatt aacaattgag taggcgagca caccttcctg atgccgctga tgctgttcca
    12781 tacgcgcctt caacacttct gcgcgctgct cgcgccgttt aatcagggca ttaaacgcgc
    12841 cgtacagtcg cgccttcacc gcacacaact gcgccgcgtc ggtttccgcc gccgcgtaat
    12901 cgtgaaactg gcgtatcaac tgctgatgca gtgctggaag gcgtcgccag cgttgcttgt
    12961 cgagagcttc attcatcgcc tcgaccagtg cgaaaagttg cctgcgttgt ttttccatcg
    13021 tcttaaccca gcttgcccga aagcccttcc cagccttcgc gcaggttgct taagatcagc
    13081 atcacttcat cgattttctc tgccgacagt tcgccgctgg cctcatacaa tcggtaaacg
    13141 cagtggtcat agagtcgcga aaggttgacc accagctcgc cgccagtttc aaactccagc
    13201 gaactggtga gcgcattgag aatgtcgatg catttattga tgctctgggc tttcttctcg
    13261 aaacgccgcc cttcaatatg gcttttggcg cgttccagtt cgtccatcag gccagaaaac
    13321 agcaccagca ccagttccag aggcgatgcc gccgcagtac gcgccgcgag atcgatctct
    13381 ttatattgcg aatagccgtc ctgtacttgg tacatcttta gggatattcc ttctgagtta
    13441 cgcgaatgcc gccatgctgg ctttcatggt gtacacctcg accagcgtgt tggtgtactc
    13501 ctcgagataa cggtcgtagt tggcgttata ggtatcagta agctgatcgc cttcgtcctg
    13561 aattttgctc atctgatcgt caatgttctg ctggcgcagg gtgatgatgc cgttgctgga
    13621 gtccgtgtag gtgttaataa ggtcgtccat ctgcgccacc atgctgttgt cgccaacgaa
    13681 aatagaggtc agaccgtcag gatttttcgc catttcgtcg ttgaactggt cggagtcaat
    13741 ctgtaagtgg ccgtgagaat cgagggtgat gccatagtca acaatcgaca cgccgttgta
    13801 gctggcatgg gcgatgtcat cgagctggtt cgccagtgaa ctgagtccgg cgtcgcctgc
    13861 aaataccccg gcgctggtac tgtcatcacc gtgggtggtt agcgaatcaa ccgtatcaat
    13921 caaggtgtta taggcatcga caaaggtctg tactttctcc tggctggcgc tggaatcttc
    13981 gctgatgtta aaggtagtga gatcgctgtc agaatcgctg acttcggtga aagtcatggt
    14041 gacaccgggg atcacatcat caaacgtatt gctgctgttg gtgatctcag gccccgtggc
    14101 gctgccaaga tgaataatcg cgtcctgagc ggaggaaaca tccgtggcaa cgggcgcgct
    14161 ggtgctatcg ttgctggcat catgccccgt tacgctcacc gaaaacgcgc tctgcgcccc
    14221 ggtgctatcc gaggtgagca taatcgttgt ggtgccgtcg gtttttacca gcgccgccga
    14281 cacgcccgga ttatcatcgg agtcgttaat cgcattcacc agttcactgg cgtcgataaa
    14341 accgtcgccg tcaccattct gatctgccgc cgacagatcg atatccatgg tgctgtcgcc
    14401 catcgtcagt tcaaaagtgc cggtggcgga aaaggcgtcg tcgcccatac tgaacgtggt
    14461 ttgctgcccc tgtgccagtt gctcaacgaa aaatgagtag cttcccgcct gcgcctggga
    14521 attggcggaa acggtcgccg agtcattatt gctggtggcg gcaaacgtca ccgggccgtc
    14581 ggtatcgctg ttcagcgcat cgacggcgct ctggaaatcg ctcaacgctg agctgagcga
    14641 gtccaggccg ctgctttcag catccagctc tgtttgcttc tcctgcaagt ttgccgcctg
    14701 agtggcgaca tccgcatacg cgatttcttg cgcaatggtt cttgggttga tcattgatga
    14761 ctccggataa cgatgtcagg ttattggcaa aaggcgtgcc aatatttttt atgtgaataa
    14821 tcaatagatt aaaaaacagg cggaaatggc gtttccggtc tggcggaaaa aagcctgccg
    14881 cagaaagcac aacaccgccc gcaggcggtg ttgttcgtag gccgaataag gcgtttacgc
    14941 cgcatccggc aagtccgcca ttactgcagc aggctggaaa ccatgctgga catgctgttg
    15001 gactgtttca gcatggtgat gccagtctgc atcagcacct gctgtttggt catattggac
    15061 gcttccgtcg cgtagtcggt atccataatg ttaccgatag cgacttcggt gttgtcctgc
    15121 atactggtca ggttgttggc ggtgtcgttc agacggttga tagacgcacc cagcttggac
    15181 tggatctgcg aaacgtcatc catcgcggta gagatgctgg tgatcatctg gttagccgag
    15241 ccagacgcgg tcagctcagt accgccagaa acacctgcag tacccgtggt gtctgcctga
    15301 tccgcgctaa aggagttgct gatagcagaa agatcggtca ccagcgtgtt cagttggctg
    15361 gagatgttca cggtcatggt gtcggaagat tccgcgccca cctggaaagt caccgcgctc
    15421 tggaacagac cgtcagtacc ggtgttgctg gtgccggaaa caccgaacag gttggtgccg
    15481 ccgtaggtgg tgttttgcag catgtcagac atctgctgac ccagttcgtc gtattcgtcc
    15541 tgcatcgcct gcagatcgtc gtcgctgtag gtgccgttcg ctgcctgagt tgacaggtct
    15601 ttcatacgac ccagcacatc agacatttca tcgaacatac tatcggcggt ttgcagcatc
    15661 gcggttgcgt cattgatgtt gctcagcgcc acgcccatac cgctggactg tgctgtcaga
    15721 cggttagcaa tttgtttgcc tgcggcgtca tcggcagaag agttaatccg gttacctgtc
    15781 gccaggcgtt ccatagatgt agacaaagaa gagctgcttt tgctgattgc attaacggcg
    15841 gccattgagg cgttattggt gttaatagat aacatgatac tgctccttgg gattaatcgt
    15901 tttcgtttcg ataccctgta aaaacgacac ctaaggagca aacttaaaac cgccgttaaa
    15961 aaaatttcac tgtgatttat ctgatgccac ttttagctaa aaaaagcaga gtttaaattc
    16021 agataaaaag attattagca atatctgcca catgg
//
LOCUS       DAPPNJ010000103        15747 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000103 DAPPNJ010000000
VERSION     DAPPNJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.104"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            122..664
                     /locus_tag="RPM73_002719"
     CDS             122..664
                     /locus_tag="RPM73_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521064.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            764..1966
                     /locus_tag="RPM73_002720"
     CDS             764..1966
                     /locus_tag="RPM73_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDX6521065.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2021..2491
                     /locus_tag="RPM73_002721"
     CDS             2021..2491
                     /locus_tag="RPM73_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDX6521066.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2532..3434)
                     /locus_tag="RPM73_002722"
     CDS             complement(2532..3434)
                     /locus_tag="RPM73_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDX6521067.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHGRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3628..4818)
                     /gene="glpC"
                     /locus_tag="RPM73_002723"
     CDS             complement(3628..4818)
                     /gene="glpC"
                     /locus_tag="RPM73_002723"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDX6521068.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4815..6074)
                     /gene="glpB"
                     /locus_tag="RPM73_002724"
     CDS             complement(4815..6074)
                     /gene="glpB"
                     /locus_tag="RPM73_002724"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDX6521069.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADNKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6064..7692)
                     /gene="glpA"
                     /locus_tag="RPM73_002725"
     CDS             complement(6064..7692)
                     /gene="glpA"
                     /locus_tag="RPM73_002725"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDX6521070.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7965..9323
                     /gene="glpT"
                     /locus_tag="RPM73_002726"
     CDS             7965..9323
                     /gene="glpT"
                     /locus_tag="RPM73_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDX6521071.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9328..10404
                     /gene="glpQ"
                     /locus_tag="RPM73_002727"
     CDS             9328..10404
                     /gene="glpQ"
                     /locus_tag="RPM73_002727"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDX6521072.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10446..10685)
                     /locus_tag="RPM73_002728"
                     /pseudo
     CDS             complement(10446..10685)
                     /locus_tag="RPM73_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10867..11517
                     /gene="inaA"
                     /locus_tag="RPM73_002729"
     CDS             10867..11517
                     /gene="inaA"
                     /locus_tag="RPM73_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDX6521073.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11571..11825)
                     /gene="yfaE"
                     /locus_tag="RPM73_002730"
     CDS             complement(11571..11825)
                     /gene="yfaE"
                     /locus_tag="RPM73_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDX6521074.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11825..12955)
                     /gene="nrdB"
                     /locus_tag="RPM73_002731"
     CDS             complement(11825..12955)
                     /gene="nrdB"
                     /locus_tag="RPM73_002731"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDX6521075.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13102..15387)
                     /gene="nrdA"
                     /locus_tag="RPM73_002732"
     CDS             complement(13102..15387)
                     /gene="nrdA"
                     /locus_tag="RPM73_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDX6521076.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3656 a         3903 c         4289 g         3899 t
ORIGIN      
        1 agaagtattt agttacaata agtgactaga tccctttttt cgctcattga atcctctgtg
       61 ccaggcagta caatcccgca gctttgggaa ttttgattaa ttgcttgtgg gaaaggggat
      121 aatgaaaaaa attgcgctag caggtctggc cggaatgctg ttggtttctg catcggtcaa
      181 tgcaatgagc atcagcggcc aggcgggtaa agaatacacc aatattggtg tcggttttgg
      241 gactgaatcg acgggcctgg ctttaagcgg taactggaca cataacgacg acgacggtga
      301 cgtcgcgggc gtggggctgg ggttgaatct gcctctcggg ccgttaatgg cgaccgttgg
      361 cggaaaaggc gtttacacca acccgaatta cggcgatgaa ggttatgccg cagcggtagg
      421 aggtggtttg cagtggaaaa ttggcaacag cttccgtttg tttggcgagt attactactc
      481 tccggattcg ctctccagcg gtattcaaag ttatgaggaa gcgaatgctg gcgcgcgtta
      541 caccattatg cgtccagtca gtattgaggc gggttatcgc tacctgaatc tgtcgggtaa
      601 agacggtaac cgcgacaacg ccgtggctga cggcccgtat gttggggtta acgccagttt
      661 ctgatccctt ccggcgcggc attctgtcgc gccattcagt ttcttactac gccttatctc
      721 cctttgcgtt atagtgattc gataacaaaa gcaggagata accatgttaa aagtggaaat
      781 gctatccacc ggggatgaag tgttacacgg gcaaatcgtt gacactaacg ctgcctggct
      841 ggccgatttt ttctttcatc aggggctgcc attatctcgc cgcaatacgg tgggggataa
      901 ccttgatgac ttagtcacca ttcttcgcga acgtagtcag cacgccgatg tgctgatcgt
      961 taacggcggg ctgggaccga ccagcgatga tttaagcgca ctcgccgctg cgacagcaaa
     1021 aggtgaaggc atggtgctgc atgaagcctg gctcaaagag atggaacgct atttccacga
     1081 acgtggacga gtaatggcac cgagcaaccg taaacaagcg gagctgcctg ccagtgctga
     1141 atttatcaat aacccggtag gcaccgcctg tggttttgcc gtgcagctta atcgttgcct
     1201 gatgttcttt actcccggcg taccgtcaga atttaaggtg atggtcgagc acgaaatcct
     1261 gccgcgtctg cgcgagcgtt tttctttacc acagccgccg gtttgtctgc gtttaactac
     1321 ctttggtcgt tcggaaagcg atctggcaca aagcctggac actctacaac tgccgccggg
     1381 cgtaacaatg ggctatcgct cttcaatgcc gattatcgaa ctgaaactca ccggaccggc
     1441 aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt aaacgtgttg ccggacagag
     1501 cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc agtcgcgaat tgcaaaaccg
     1561 ccagttcagc ctgacgttga gcgagcaatt caccggcggt ttattggctt tgcaactttc
     1621 tcgcgcaggt gctccattgc tggcgtgtga agtggttcct tcacaggaag aaaccctggc
     1681 gcaaactgcg cactggatta cagaacggcg ggccaaccat tttgccgggc tggcactggc
     1741 tgtttcgggt ttcgagaacg agcatctcaa ctttgcgcta gccacgccag acggcacttt
     1801 cgctctgcgt gtgcgtttca gcactacacg ctacagcctg gctatccgtc aggaagtgtg
     1861 cgcaatgatg gcactgaata tgctgcgccg ttggttaaac ggccaggata tcgccagtga
     1921 gcatggctgg attgaggtta ttgagtccat gaccttatct gtctgattcc gccactctgt
     1981 gccggtttac tgcaccggca cattccccct ccggcaaaac ttgtccaacc ctccctacgg
     2041 tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta
     2101 catggcgcaa gttaacgatt cactttgtct gttggtgcag gtggaaagta aaactgcact
     2161 ggataacctg gacgaaatcc tcgacgtcga agggattgat ggcgtgttta ttggacctgc
     2221 ggatctttct gcgtcgttgg gctacccgga taacgcaggg cacccggaag tgcagcgaat
     2281 tattgaaacc agtattcggc ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt
     2341 ggctcctgat atggcgcagc aatgcctggc gtggggagcg aactttgtcg ctgttggcgt
     2401 tgacacgatg ctctacagcg atgccctgga tcaacgactg gcgatgttta aatcaggcaa
     2461 aaatgggcca cgcataaaag gtagttattg atatcaaagg cccatgggga tcggctgtgg
     2521 gcctgtgtta attagtggtt attcgctgcc agatcggctt cgcttagctg ggtggccgcg
     2581 agcacctggt cacgatcaat gccctgttcc agcatgcgca aagcaatttt aatggcttgt
     2641 tcatgcatac cttcctgcca gccaatttga tgcccttcct gccgtagccg ttcagcaata
     2701 gtcattaatc tctccttttg tagtggtgaa cgttcggcaa tctcctcaat aaaacgggtg
     2761 aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctggctgtca
     2821 ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga ccatgccgat taaatcgcgg
     2881 tcgcgaatat gcttttgaat cagttccagc agagctatac gcccatgttg catgatctca
     2941 tcgtcaggta cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca
     3001 agttgcggat cgtcaaactc atccagccag ttaagtgagt acgggtaggg cgatgtttcg
     3061 ccatgataaa acagcaatgg caccaccagc ggaactctgt catagccttt atccaggtga
     3121 cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt ttctgcagag
     3181 ctttgatgtt caatcacgca gtagatatag ccgtcaccgt cgctggtttc cacggaccac
     3241 aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactagtggg ttccaggcgt
     3301 aaggtttgca ggttgcaaag cttgcgcagt ggttctggta aatgtatttc gagaaaatcc
     3361 cgtgcggttt cgggtgtgaa cataaaggtt ttaaacaccg catcatgcgg ggaggatgtt
     3421 gttgattcgg tcatggttgt ccgtcacctg atatttatga tgacgcgaca ttacgtctat
     3481 cccgggcctt tgacatctgt cagtttcagc ctttgcgaga cattttcaga ggaattttca
     3541 aagcgttgca acgcatacat ggtgcgtgga atcgcgctcg caaaaatgcc cgataagcaa
     3601 tgtgcttacc gggcatcaaa aggtcggtta agccagcgcc tgggccagta gcgtaatcgg
     3661 atgttcgcag cgaaggctcg tggacatctc aatctgccat ttgcaggttt cgcagtcggt
     3721 gatgaccaga tccgcgccgc tctcttctat ctggcggaac agtggcgcac cgatggcttg
     3781 tgaggtgggg tagttctctt ttttgaaacc gtaagtaccc gcaataccgc agcactggga
     3841 atccagcacc gttaactcaa gccccgggat tttacgcaac agctccaggg tgtagagcgt
     3901 ccagcccatt ttttccatat ggcacggagt gtgataaacc actttcagcg gcagcggttt
     3961 cagcggtaac gttttgcctt cgtccagctt gcgccacagc cagcgggttg ccagttcgat
     4021 atgatcgcgc aagcctttgt tgtcgacatt cagcacttcc gggtattcgt cgcgcagggc
     4081 aaatgtacag gttgaggagg tggcaatcac cggaatgcct tttactccca cagcttcgcg
     4141 gatcgactct acattcgtaa ttgcctgttt gcgtgcttta tcggtaaagc cgttggcgat
     4201 tagcggtacg ccgcagcatt tttctttgct gagcagttgt acaccggtac ccattgcgtt
     4261 gagcacttta attaaatctt tacctaactg cggatggttg tagttaacga agcagccgtg
     4321 aaagaaagcg acctggtctt tatattgtgc ttgctgagcc gctatgctgc gataccagcg
     4381 acggaacgtg ccgaaggagt atttcggtag cgtgcggcga tgatcgattt ttaacgccgc
     4441 atcaagcagc tgccgcaccg gtttcagcga ggtagcggtg ttgacgattg gtgcgaacgg
     4501 cgtggaaacg ctacccatca ggtcggtatg actcaacaca aaattacgca gcgacgggcg
     4561 cgtggtgtca tattttgccc gcgcgcgctg gataatatcg ccaatcttca catcggacgg
     4621 gcaggcgact tcacaacgtt tgcagttgat gcaatatttc agcgcctcgt catacagtgc
     4681 gccatctttc aaacgcagac gctcgccatc cggcccggct tgttttggcc ctggataacc
     4741 gggattcacc cggctcaccg ggcaggcggt ggtgcagacg gtgcacttaa tgcagttttc
     4801 gaagctggtg tcgttcattg ttggcctcct gcgcgttggg caatctgttg agcggcatgt
     4861 aaagcactga cggcacaaac accgccgccg catccctggg cgatgggatc aaatccgccc
     4921 agcaccgaac cgatggcaaa caggttctca atggtttgcc ctgcctgcga cgggcgtagc
     4981 gtctcatcag tggttacacc gaactgctgc cacggttgcg gcgcaaaaaa atcgccctta
     5041 taccattcac cccgcgtggc ggtttgtagc acatcaaggc cgagaatcgg ctcgcgaatg
     5101 ccgttacgtt ccgctaccag tccgccacta aagaaactgc cgctggcgag aaccgcgaaa
     5161 cgtggacgta gcggaatatc ggcgtgattg cgggtccaga tttcattcac tacgccattt
     5221 ttacaggtca cttttttcac ttcatcgccc ggcatccaca cgccaccctg gcgcacaaac
     5281 tggcgctgta actggttttg cagacgaatg cccagcacgg aaggcggcag cgttggcaaa
     5341 agcatcagtg aacaaggtag tttttcattc aaccaacgcc acagtttgtt atcggccagg
     5401 ccgaagcagg cgggcatcag gatcatttcg caggtattgg cgacaggaat aagcgcatca
     5461 agtaacagcg gccagttttc ttcattatca aggaaacggg cgatattcac agcgcgaaat
     5521 tcggtggcgt tattgcgcag gacatccagt tccggcagct ctatttctgc ggtttcaacc
     5581 gaaagatcca gttcgcgcaa cgaagccgcc gcaagatgcg cctggaaatc catcagcccg
     5641 cttatcccca ctacacatat cttcttcgcg ggtagcggcc agacgggaac ttctggcgaa
     5701 cttaaccagg ttgcacgcaa agtgccgagc ggggtaacgc gctgatgggc cagttccacg
     5761 ctgccctgca attgcgcacc gctttcagcg atcaatgcct gcgcctggca agcgagatcg
     5821 agcacgcgtt gtggttcgag aagactgtaa ggatgggctg gtgcctgctg acgcaaagat
     5881 tccagtccac tgtgaatgtc tgtcaccggt tgaccatcag gcagatggct cagcaaatcc
     5941 agcgaaccgg atgagaaatg cagcgcgctt tgaccacgcg tgacaatggc acagcgcagg
     6001 ccgtgttttt gcagttgcag gccacagagt aatccggcga ggccgccgcc cataatgaca
     6061 gtatcaaagc gcatctttct gctccttctc cagaccacac agtccctgat aaacccagcg
     6121 ggtaaattcg ctttcgcgca gtgcgtctcc ccaggcgatg ggttgcacgc ctttccagcg
     6181 ttcgttaagg aaggtggaaa gttgctcgat agattgcgcg gacgtcgtga cgttaaaccg
     6241 ttgcagcagt ccggcagcgc ggcaggcgca gagttcgccc tggcaggtac ccatccccac
     6301 acgggtacga cggcgtaaat ccagcaggct attaacgttt aaattttcta ccgcgtactg
     6361 aacttcaccc gcagttaccg cttcgcactc acataccagg ctacgatgca gacggccttc
     6421 gctcagccag gcaggcgtgc gatcgccatg acgataaacc gcagaaccgc gcagcggggc
     6481 aggcagggag atgactttac gcaaggtaac ttcagcgggt tcttgtgaac caggcagtgc
     6541 cagatcggca gtcgtacagg ggcgcgtgtt gcccagtttg cggcataccg cgtcggtagc
     6601 ccattcagcc atcagccgat aggtcatcag tttgccaccg gtgatggtga taaatccgtc
     6661 cagaccatcg cgttcagcat ggtcgagcag cacgatgcca cggctgacgt tacgtccgct
     6721 cgggtcgtca tcgctggcaa ccagcgggcg cacgccagaa taggcccgca aaatgcgcgt
     6781 tttcgccatc acgggggcca gtttttcccc ttcacgcagc agaatatcaa cctcttctgc
     6841 cgtcactcga ttatcgtcaa tctcgttgta atcaatacgt aaagaggtgg tgccaatcag
     6901 cgaaatggta tcgccaggca ccagaatatc ggcgtcggaa ggtttacggc agcggttgat
     6961 cacatgctgg ttaatgcggt gatccatgat cagcagcgat cctttcgccg ggaacatgcg
     7021 aatgcgcaga tcggcatatt cggcaatgtg ttgcccccag atcccagcgg cattaaccac
     7081 gacaggtgca tgaagggcct gagtttcgcc ggtgagatgg ttacgtacac gaacaccgca
     7141 caccgtcgcg ccttcacgaa tcagccccgt gacttcatga gcggtaagga taacggcacc
     7201 gtgttctttg gcatccagca tgtttgctgc ggtcagacga aatggatcaa cggtgccatc
     7261 cggaactttc accgcgccaa tcagtgccgg gttaacggca ggttcgataa tgcgcgcttg
     7321 ctgcgggtct atagcttctg cgctgatccc tgcttcttcg caggcgcgaa taaaagtggc
     7381 ctggaatgag aggtcatctt ccggcagggt gataaacagg ccgttggttg gttcaacgca
     7441 gtgacgtgca atgcgtttca ggatctggtt ttcactaatg cattcgcggg ccgattccgc
     7501 atcggttacc gcatagcgcg caccgctgtg cagcaggccg tggttacgcc cggtggcacc
     7561 ggttgcgatg tcgtggcgct caaccaaaat cacgcgcagc ccgcgcaggg cacagtcgcg
     7621 ggcaatcccg gctcccgttg cgccgccacc aatgataatc acgtcacttg attgcgagtc
     7681 gcgagttttc attgttatcc ctctgaagtt cgttttttac catttagcca tagtaaaaac
     7741 atgaattgtt tgatttcgcg catattcgct cataattcga aagtgaaacg tgatttcatg
     7801 cgtcattctg aacattttgt aaatcttatt taataatgtg tgcggcaatt cacatttaat
     7861 ttctgaatgt tttcttaaca tcgcggcaac tcaagaaacg gcaggttctc ccactgaatc
     7921 agtctgttaa tcataaataa gaccacgggc cacggaggct ttcaatgttg agtattttta
     7981 aaccagcgcc acacaaagcg cgcttacctg ctgcggagat cgatccgact tatcgtcgat
     8041 tgcgctggca aattttcctg gggatattct ttggctatgc ggcttactat ttggttcgta
     8101 agaactttgc gcttgctatg ccttatctgg tcgagcaggg attctctcgc ggtgatttag
     8161 gtttcgcgct ttcggggatc tcgattgctt atggattttc gaaattcatc atgggttcgg
     8221 tatcagatcg ctcgaatccg cgcgttttcc tccccgcagg tttgattctg gcggcggcag
     8281 tgatgttgtt tatgggcttt gtgccatggg cgacgtcgag cattgcggtg atgtttgtac
     8341 ttttgttcct ctgcggttgg ttccagggga tggggtggcc gccgtgtggt cgtactatgg
     8401 tgcactggtg gtcgcagaaa gaacgtggcg gcattgtgtc agtgtggaac tgtgcgcaca
     8461 acgttggtgg tggtattccg ccgctgctgt tcctgctggg gatggcctgg ttcaatgact
     8521 ggcacgcggc gctctatatg cccgctttct gcgccattct ggtggcatta ttcgcctttg
     8581 cgatgatgcg cgataccccg caatcctgtg gcttgccgcc gatcgaagag tacaaaaatg
     8641 attatccgga cgactataac gaaaaagcgg aacaggagct gacggcgaag caaatcttca
     8701 tgcagtacgt actgccgaac aaactgctgt ggtatatcgc catcgccaac gtgttcgttt
     8761 atctgctgcg ttacggcatc ctcgactggt caccgactta tctgaaagag gttaagcatt
     8821 tcgcgctgga taaatcctcc tgggcctact tcctttatga atatgcaggt attccgggca
     8881 ctctgctgtg cggctggatg tcggataaag tcttccgtgg caaccgtggg gcaaccggcg
     8941 ttttctttat gacactggtg accatcgcga ctatcgttta ctggatgaac ccggcaggta
     9001 acccaaccgt cgatatgatt tgtatgattg ttatcggctt cctgatctac ggtcctgtga
     9061 tgctgatcgg tctgcatgcg ctggaactgg caccgaaaaa agcggcaggt acggcagcgg
     9121 gctttaccgg gctgtttggt tacctgggcg gttcggtggc ggcgagcgcg attgttggct
     9181 acaccgtgga cttcttcggc tgggatggcg gctttatggt aatgattggc ggcagcattc
     9241 tggcggttat cttgttgatt gttgtgatga ttggcgaaaa acgtcgccat gaacaattac
     9301 tgcaaaaacg caacggaggc taatggcatg aaattgacgc tgaaaaacct tagcatggcg
     9361 atcatgatga gcactatagt catgggaagc agtgcaatgg cggcggacag caacgaaaaa
     9421 atagtcatcg cccatcgcgg tgccagtgga tatttgccgg agcatacgct gccagcaaaa
     9481 gcgatggcgt atgcgcaggg agcggattat ctggaacagg atttggtgat gaccaaagac
     9541 gaccatctgg ttgttctgca tgaccattat ctcgatcgtg ttactgatgt tgccgatcgt
     9601 ttcccggatc gggcgcgcaa agacggtcgt tactacgcga tagatttcac gctggatgaa
     9661 attaagtcgc tgaaatttac cgaaggtttc gatattgaaa acggtaaaaa agtgcagact
     9721 tatccggggc gtttcccaat gggtaagtcc gacttccggg tgcacacctt tgaagaagag
     9781 attgaatttg ttcaggggtt aaatcactct accgggaaaa atatcggtat ttatcctgaa
     9841 atcaaagcgc cgtggttcca tcatcaggaa gggaaggata ttgcggcaaa aacgctggaa
     9901 gtgctgaaga aatatggtta caccggtaag gacgacaaag tttatttgca atgttttgat
     9961 gctgatgagc tgaagcgtat taagaatgag ctggaaccta aaatgggcat ggatctcaat
    10021 ctggtacagc tgattgccta taccgactgg aatgaaacgc agcagaaaca gccggatgga
    10081 agctgggtta attacaacta cgactggatg tttaagccgg gtgctatgaa acaggtggcg
    10141 gaatatgccg acggtatcgg gccggattac catatgttga ttgaggaaac atcgcagccg
    10201 ggtaatatca aactcactgg catggtgcaa gatgctcagc agaataaact ggtagtgcat
    10261 ccttataccg tgcggtcaga taaactgcct gaatacacta ctgatgtgaa tcagttatat
    10321 gatgctctgt ataacaaagc gggtgtaaat gggctgttta ctgatttccc tgataaggca
    10381 gtaaaattcc ttaataaaga gtaatgctac tgcaggatgg cgttcatgcg ccatcctgtc
    10441 tcacatcaaa tttttccatt caccacgagt tgttccgtac acgtttgtag taaaaacagt
    10501 ggactgcctt ctaccggctt ttctttagcc aacaacgaaa tctgtcgata gaaggttggt
    10561 tcgtgaggaa cggaacacaa ttcacctgca atgcttttca gcgttaactc tggttgtaag
    10621 gcgatgccta atccctgtcg aataaacttc atcgctgtat ccgactgatt aaattcacat
    10681 tttatctact gggcgcacta ttagtggtag tggctggtgg aatctatgcg ctgcaaatga
    10741 agggtaaaag cggtgtcacg ctagtagtgg caaaagaaat tcattaatac gacacgtttc
    10801 attaagattt tcctcaggta accacctata gtcattgtcg ttaataaaaa tgacaggcga
    10861 tagggtatgg cagtttcagc aaagtacgac gaatttaatc actggtgggc aacagagggc
    10921 gactgggttg aagagcctaa ctatcgccgt aatggtatga gcggggtgca atgcgtcgag
    10981 cgcaacggca aaaagctgta tgtaaagcgc atgacgcatc atctgtttca ttccgtacgt
    11041 tatccgttcg gccgaccaac gattgtccgt gaggttgcgg taattaaaga actggaacgg
    11101 gccggtgtca tcgtgccgaa gatcgttttt ggtgaagcgg tgaaaattga gggtgaatgg
    11161 cgagcgttgc tggtgactga agatatggcg gggttcatca gcattgctga ctggtatgcc
    11221 cagcatgcag tatcgcctta ttctgacgaa gtacggcaag ccatgttgaa agcagtggcg
    11281 ctggcgttta agaaaatgca tagcattaat cgtcagcatg gctgttgtta tgttcgccat
    11341 atctatgtga aaacagaagg taatgcagaa gctggttttc ttgacctgga gaaaagtcgt
    11401 cgccgcttgc gtcgcgataa agcgattaat catgacttcc gccagctgga aaaatatctg
    11461 gagccaatcc ccaaagcgga ctgggagcag gtgaaagcgt attactacgc catgtaatcg
    11521 ttgtaggcca gataagacgc ataagcgttg tatctggcac tacaaccaat tcacatctcg
    11581 atttcaatat cgccttttgc ccggcaacaa cagggcaaaa tttcccccgg ctgaataaag
    11641 gctaacggtt cggcaatcca gtcaacctga cctgcaacca gacgggtgcg acaggagccg
    11701 cagtaacctt cgcgacactg atactcaacc gccacattgt gggattccag cgccgccaga
    11761 agggaagggt gttcatcctg gcacagcagt tgtgtgccag tgatgcgcag ggtaacgcgg
    11821 gccatcagag ttggaagtta ctcaaatcgt cggtgtccac ttccgcgtca atctgcccga
    11881 ccagataaga actgacttcc acttcctgcg gagcaacctg cacgttatca gacaccagcc
    11941 aggtgttgat ccatgggatc gggttggagc gcgtctggaa cggcagatcc aaaccgactg
    12001 cctgcatacg gatattggtg atgtattcaa cgtactggca gagaatgtct ttattcagac
    12061 caatcatcga accgtcgcgg aacagataat ccgcccagtc tttctcctgt tgagctgcct
    12121 gaacaaacag gtcatagcac tcctgcttac actcttcggc aatttccgcc atctcaggat
    12181 cgtccgcgcc gctgcgcagc agattcagca tatgctgggt gccggtcagg tgcagggctt
    12241 cgtcgcgggc aatcaggcga ataattttgg cgttgccttc catcaattcg cgttctgcaa
    12301 atgcgaagga acaagcaaag ctgacgtaga aacgaatcgc ttccagcgcg ttaacgctca
    12361 tcaggcagag atacagtttt ttcttcagct cgcgcaggct aacggtcaca gttttaccgt
    12421 taacggtgtg ggtaccttcg cccagcagat gccagtagct ggtcatttcg atcagctcat
    12481 cgtaatagct ggagatccct tccgcacgtt tctggatctg ctcgttggtg acgatatcgt
    12541 caaacacaac agacggatcg ttaacgatat tacgaatgat atgagtatag gaacgggaat
    12601 gaatcgtttc tgagaacgcc caggtttcga cccaggtctc cagttccggg atcgagatca
    12661 gcggcagcag tgccacgttc gggctacggc cctgaatgga atccagcagc gtctgatatt
    12721 tcaggttgct gataaagatg tgtttttcgt gttcaggcag tgcctggtaa tcgatacggt
    12781 cgcgagaaac gtcaacttct tccggacgcc agaagaaaga gagctgcttt tcgatcagct
    12841 tttcgaagat gtcatatttt tgctgatcgt agcgagccac gttgaccggc tgaccaaaga
    12901 acatcggttc tttgagctga tcatttttcg tctgtgaaaa ggtggtatat gccatgagtg
    12961 tgtcctgttg ggagtttaat gccggataag gcattttacg ccgcatcctg tgcctgatgc
    13021 gacgctgacg cgtcttatca ggcctacaaa tccgagccgt aggccggata aggcgttcac
    13081 gctgcatccg gcatctcaat atcagatctt acatgcgccg ctttcgcagc catcgtcctg
    13141 gattgacggt accagatcgt cctgcgcgtc ttcagcgccg tcacgggtgt tctgataata
    13201 cagcgttttg accccgaatt tgtaggcggt gagcaggtct ttcagcaact gctgcatcgg
    13261 cacttttcct gacgggaaac gtgacggatc gtagttggtg ttagcagaga tcgactgatc
    13321 gataaatttc tgcatgatac ctaccagttg caggtagcca tcgttacccg gcatttccca
    13381 cagcagctca taggcgtcgt gcaggtgctc gtagtccggc accacctggc gcaaaatacc
    13441 gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt tcaataccgt tagtggcgtt
    13501 agagatctgt gaagaagtct ctgacggcat cagggcagaa agcgtggagt tacgcaggcc
    13561 gtgcgttttg attgattcac gcaatgcttc ccagtcgtaa tgaagcggct cattagcgat
    13621 ggtatccaga tctttcttat aggtatcgat cggcaggatc cctttcgcgt aagtggtttc
    13681 gttaaaccac gggcacgcgc cttgctcttt tgccagctcg ttagacgctt tcagcaggta
    13741 atactgaatc gcttcgaagg ttttatgcgt caggttgttg gcgctgccgt cggagtaacg
    13801 cacaccgtgc ttcgccaggt agtaagcgaa gttgatcaca ccaataccca gcgttcgacg
    13861 acccatcgct ccacgtttgg cggccgggat cgggtaatcc tgataatcca gcagcgcgtc
    13921 aagtgcacga accgccagaa ttgccagctc ttccagttca tccaggctat taattgcgcc
    13981 caggttgaaa gcagacagcg tacacagcgc gatttcaccg ttctcgtcgt tgacgtcgtt
    14041 cagcggtttg gtcggcaggg ctatctccag gcacaggtta gactgacgca ctggcgcgat
    14101 ggccggatca aacgggctat gggtattgca gtggtcaacg ttctgaatat agatacgacc
    14161 ggtagacgca cgttcctgca tcatcagcga gaacagctca acggctttca cacgctgctt
    14221 gcggatgctg tcgtctttct catatttggt atacaggcgt tcaaactctt cctgatcggc
    14281 gaagaatgcg tcgtacagcc ccggtacgtc ggacgggctg aacagggtga tatcttcacc
    14341 tttcagcagg cgggtataca tcagtttgtt gatctgtacc ccgtagtcca tatggcgcac
    14401 gcggttgcct tccacaccac ggttgttttt caacaccagc aggctttcca cttccagatg
    14461 ccacatcggg tagaacagcg ttgccgcacc gccgcgcacg ccgccctgag agcaggattt
    14521 caccgctgtc tggaaatgtt tgtagaacgg aatacagccg gtatggaacg cttcaccacc
    14581 gcgaatcggg ctacccagcg cacgaatacg cccggcgttg atgccgatcc cggcacgctg
    14641 ggaaacgtat ttcacaatcg cgctggaggt agcgttgatg gaatccagac tgtcaccgca
    14701 ctcgatcagt acgcaggagc tgaactgacg agtcggggta cgcacgccgg acatgattgg
    14761 cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa aaacgcttca catattgcag
    14821 gcgcgtttca cgcgggtagt tcgagaacag gcacgcggca accagaatat aaaggaactg
    14881 ggcgctttcg tagatttcgc cggtcacgcg gttctgtacc agatatttgc cttccagctg
    14941 cttaacggca gcataagaga aggtcatatc acggtcgtga tcgataaacg tgtccatctg
    15001 cttgaactct tcttccgtgt agtcttccag cagatgatta tcgtatttgc ccatctcgac
    15061 cattttcacc acgtggtcgt acagcgcagg cggctcaaac tggccgtagg cttttttacg
    15121 caggtggaag atcgccaggc gcgcggcgag atactgataa tccggcgcat cacgggagat
    15181 caggtctgcg gccgccttga taatggtttc gtggatgtca gaggtcttga taccgtcata
    15241 aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa acgttatgca gtccttctgc
    15301 cgcccaatcc agaacgcgat ggattttgtc gagattgatg cgctctgtgc taccgtcgcg
    15361 ctttgtcacc agcagattct gattcatgta tgtcgtacct gtttttggaa ttctttccgc
    15421 aatagtttca tgcagactac ctgtagtacg ggaagctgct tagaacacta tatatagggg
    15481 gtatgtttgg gattcactgc aagatagtgt gaaaatggcc ctcttgcaag tgcataactt
    15541 tgtggataac tcaggaagga aaaagtggct ttcgcgcacc ttaggtcaga caaggtgtcc
    15601 gggaaagtca atgggaagag aaaaatttgt taaaaataac tgttcggcga tttcttgaac
    15661 ggcagaatag gttggataaa ttgatataga tggtttatta atctttttgc taatgattgc
    15721 aaaaattgat ctaagcacga gtcatca
//
LOCUS       DAPPNJ010000104        15734 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000104 DAPPNJ010000000
VERSION     DAPPNJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.105"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            118..702
                     /locus_tag="RPM73_002733"
     CDS             118..702
                     /locus_tag="RPM73_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6521077.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            807..1547
                     /locus_tag="RPM73_002734"
     CDS             807..1547
                     /locus_tag="RPM73_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDX6521078.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1582..4182
                     /gene="htrE"
                     /locus_tag="RPM73_002735"
     CDS             1582..4182
                     /gene="htrE"
                     /locus_tag="RPM73_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDX6521079.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4199..4768
                     /locus_tag="RPM73_002736"
     CDS             4199..4768
                     /locus_tag="RPM73_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6521080.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4783..5385
                     /gene="yadL"
                     /locus_tag="RPM73_002737"
     CDS             4783..5385
                     /gene="yadL"
                     /locus_tag="RPM73_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDX6521081.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5412..6008
                     /locus_tag="RPM73_002738"
     CDS             5412..6008
                     /locus_tag="RPM73_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6521082.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6061..7335
                     /locus_tag="RPM73_002739"
     CDS             6061..7335
                     /locus_tag="RPM73_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDX6521083.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7448..8242
                     /gene="panB"
                     /locus_tag="RPM73_002740"
     CDS             7448..8242
                     /gene="panB"
                     /locus_tag="RPM73_002740"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDX6521084.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8254..9105
                     /gene="panC"
                     /locus_tag="RPM73_002741"
     CDS             8254..9105
                     /gene="panC"
                     /locus_tag="RPM73_002741"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDX6521085.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9179..10081)
                     /gene="rpnC"
                     /locus_tag="RPM73_002742"
                     /pseudo
     CDS             complement(9179..10081)
                     /gene="rpnC"
                     /locus_tag="RPM73_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
     gene            10355..10735
                     /gene="panD"
                     /locus_tag="RPM73_002743"
     CDS             10355..10735
                     /gene="panD"
                     /locus_tag="RPM73_002743"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDX6521086.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10739..11968)
                     /locus_tag="RPM73_002744"
     CDS             complement(10739..11968)
                     /locus_tag="RPM73_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDX6521087.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTLVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(12032..12472)
                     /locus_tag="RPM73_002745"
     CDS             complement(12032..12472)
                     /locus_tag="RPM73_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX6521088.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12577..13347)
                     /locus_tag="RPM73_002746"
     CDS             complement(12577..13347)
                     /locus_tag="RPM73_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521089.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13344..14270)
                     /locus_tag="RPM73_002747"
     CDS             complement(13344..14270)
                     /locus_tag="RPM73_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6521090.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14379..15041
                     /gene="can"
                     /locus_tag="RPM73_002748"
     CDS             14379..15041
                     /gene="can"
                     /locus_tag="RPM73_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDX6521091.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15082..15618)
                     /gene="hpt"
                     /locus_tag="RPM73_002749"
     CDS             complement(15082..15618)
                     /gene="hpt"
                     /locus_tag="RPM73_002749"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDX6521092.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
BASE COUNT         4355 a         3723 c         3581 g         4075 t
ORIGIN      
        1 tttggtaaca aatatcattg ctcattaata tctagcatct gcttataaca tttgtgtcat
       61 gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag gatttttatg
      121 tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc aggtactgct
      181 tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga taacacctgt
      241 gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac tgcaactgtt
      301 ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa acctttcagc
      361 atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa aatgaccttc
      421 ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc tattaacaac
      481 ccttctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat caaacaggtt
      541 caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac aaaatctgct
      601 gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc aactgctggt
      661 tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac caattaaatt
      721 gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt attccctgtt
      781 ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca acagctttat
      841 gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc atttcgggta
      901 ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga
      961 ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct gagcctggca
     1021 gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc
     1081 aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga gagagcgtgt
     1141 tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa
     1201 gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg gatggattga
     1261 agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta
     1321 aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc agtggtgatt
     1381 tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca tttagtgatg
     1441 aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta catttttacg
     1501 ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca ggcaaggata
     1561 taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca taatcatctg
     1621 acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg tgctgaactt
     1681 gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga tctcagccgg
     1741 tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta tgtcaacgat
     1801 caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa aaacgcacaa
     1861 gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga tataaataac
     1921 gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa tttgacggaa
     1981 attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga tatagacgtt
     2041 cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt atgggaaaat
     2101 ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga aactcctggt
     2161 cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg agcatggcga
     2221 ctgcgtgcct caggcaacta caactggatg accgattctg gcagtaatta tgattttaag
     2281 aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct tggtgagtct
     2341 tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt atacagcgac
     2401 agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg cgttgctaat
     2461 accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac gactgtgccg
     2521 ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga tcttattgtt
     2581 actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc atcggtcgtt
     2641 caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt cttaaaagat
     2701 gatattcagg atgagcctaa tttatttcag gccagctact actacggtct gaataactat
     2761 ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg tttgttaggt
     2821 cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc caatgttcgt
     2881 ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa caagttattc
     2941 gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca gaattacctt
     3001 ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca agatcttgaa
     3061 ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacggtcag tattaaccaa
     3121 ccgctaaaat ttgagaaaaa agattacggt tcattttatc tttccggaag ttggtccgat
     3181 tactgggctt ccggacaaaa tcgtagcaat tactctattg gctacagtaa cagtgcatcc
     3241 tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga cactgacgat
     3301 agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga acaacggact
     3361 tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa taaccaactc
     3421 aacgtcagta gcagtggcta tagtgataac gctcgcctca gttatagcgt aaatactggc
     3481 tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc cagctatgaa
     3541 tcaccgtggg gaaccctggc aggttcagtt tctgcaaata gcgataacag tcgtcaagtg
     3601 tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt cagtaatgac
     3661 agttttagcg actccgatac actggcggta gttcaggctc caggtgctca aggagcgcga
     3721 ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag cgctctttct
     3781 ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga tgttgaatta
     3841 aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc tgattttgaa
     3901 accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa aaatattcca
     3961 tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg acagggtgga
     4021 caagcatttg ttcgtggcat tgagcagcag ggaaatatca gcattaaatg gctcgaagaa
     4081 agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga aaaaatagcg
     4141 caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg gacgtcaaat
     4201 gataaaaata acgccacata aaatagcgat actgatggga atattattat caccatcagt
     4261 atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa cctgtaacat
     4321 cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat tgagaatccc
     4381 taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg attttaaact
     4441 ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga ccggaaatgc
     4501 atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt cgacgacaag
     4561 taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc ttaaacctaa
     4621 cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg gtcttgaaat
     4681 gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt ttcgtgcact
     4741 ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt taaaaattta
     4801 cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt accctcttat
     4861 gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg tacttgtacc
     4921 gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg cgatgtatat
     4981 atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt caaagactgt
     5041 gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac atgcgaaggt
     5101 actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa agcaagtgcc
     5161 gtcgccgttg aagtctggag caccgaaact ccggcaacag gtagcgcaac acaatttagt
     5221 tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc ggttgtttat
     5281 tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa tgtcactgcg
     5341 ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac tgacagatcc
     5401 aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt ctgtttctgt
     5461 cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac cttgttgcca
     5521 atgtaaaaaa cagcacttgc aaaagcggaa tcagtaacca gggtaatatt gatcttggcg
     5581 tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa ccaggtggaa
     5641 aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat gtgcttaatc
     5701 agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca aggcaaatat
     5761 ttgccaatga agtcgcaacc ggggctaaaa atgtcggtgt cgttctcttt tccattcagg
     5821 acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc gtgtatccag
     5881 taatgacttc tgccctgcat aattcttcat ggaaatttta tgccagaatg caaaaaatcg
     5941 atcccgcgct ggatgtcata tctggccagg taatgagtca tatattagtt gatgtgtact
     6001 acgaataata gatcaaaaca aataactgcg actgttacag cagtagatgg tggagtgata
     6061 atgaagaaat tcttcagaca ttttcttttt ttaattctgt gcttgagttg ttataccgcg
     6121 agtgctggca ctgatgataa cgtaggatat attgttggga atagttacgg cgttggtccg
     6181 tcagatcaaa aatggagaga aacggggccg aatggcgatg ctactgtcat attcagatat
     6241 gcaacaagta caaataattt agttttctac aaaccgacgc agcttggacc tacaggtgta
     6301 aaattacagt ggagtcagtt agataccgct tctggtggtg gttttcttta ttgcaacaga
     6361 agtgatagca caagtggtag cgcaatgcgt attgaaaatg caatggttga ctcaggtaaa
     6421 atgtatggct cccataaatt atttaataca tcagttcctg gtttgtatta cacattatta
     6481 atttcaaaca tgtggtcagc ttacggtacc gtaactaacg ttagttcacc tgggatatac
     6541 attggtgact ctgcagaaca atatttttcg tggtataatc caagcgaaga cgtgttatat
     6601 tggagttgca ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg tatttatcag
     6661 acccttacaa ttgaattcta tacagataca aactttgatc caacagtaac tcaacagatc
     6721 aaattatcca gctcatcaaa ttatctgtat tcatttaaag cctatggcgc aggccaaggt
     6781 ataaatgagc atagttattt tatcaaaatc gatttcgatc tgctcaatgt caagttgact
     6841 aatcccactt gttttaccgc tatgcttagt ggaacttctg tgactggttc tacggtaaaa
     6901 atgggtgaat atagtgcaga acaaatcaga aacggtgcca caccggttcc ttttgatatt
     6961 tcacttcaaa actgcgttcg tgtgactaat attgagacaa aattagtttc aacaaaggtt
     7021 ggtactgaaa acgggcaact ccttggtaat actctcacag gtaatgacgc agcaaaagga
     7081 gtcggtgtac tcatagaagg tttagcaact agtaaaaatc ctctaatgac attgaaacct
     7141 aatgattcaa attctgttta taaagattac gacccgagag gcaaagacga tacaacagga
     7201 ggagtttacc cggatcaaga tacaggtata acataccctc tccatttcca ggccacgcta
     7261 caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag tactttccag
     7321 gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg taattgataa
     7381 atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc atttatcagg
     7441 acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg
     7501 tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg atgaaggact
     7561 taacgtcatg ctggtaggcg attcgctggg catgacggtt cagggccacg actccaccct
     7621 tcccgttacc gtcgaggata tcgcctatca taccgccgcc gtacgtcgcg gcgcgccaaa
     7681 ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac aagcctttga
     7741 aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg gcggtgagtg
     7801 gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg
     7861 tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc gcggcgatga
     7921 agcaggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg cacagctgct
     7981 ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac tggcgatccc
     8041 ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc
     8101 cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg ccgaaacggg
     8161 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg
     8221 cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg
     8281 ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact
     8341 atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
     8401 gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga agatctggca
     8461 cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa agtggattta
     8521 gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca tacttacgtt
     8581 gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca ttttcgcggc
     8641 gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc ctgcttcggt
     8701 gaaaaggact ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat gggcttcgat
     8761 attgagattg tcggtgtgcc aattatgcgc gccaaagacg gtctggcgct aagttcccgt
     8821 aacggttatc tgacggcgga acaacgcaaa attgcgcctg gtctgtacaa agttttaagt
     8881 tcgattgctg acaaattgca ggctggggaa cgggatctcg atgaaattat taccattgcg
     8941 gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg cgatgccgac
     9001 acattgctgg aagtttctga aaccagcaaa cgggcagtaa ttctggtagc cgcctggctt
     9061 ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg cgtaatactt aactggcgct
     9121 acggctgatg gcgccagtta ttaatttacc ccacgtcatc tgtcataaca caggtaactt
     9181 aaataaggtt aattacctta tcaatctcag caagagataa atttgtcatc tctgcaacgt
     9241 cttcccgaga cattccttta cttagaagac gcagggcgaa ttcttgcctt tcctcctgtc
     9301 ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca
     9361 tagactcccc tcccgtttcc ctgtttctca gcacaccata aaacaaatcc gcttattcag
     9421 tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc
     9481 cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat
     9541 gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg
     9601 tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgtgcc agctccggcg
     9661 agtaaaatat atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa
     9721 acagtatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg
     9781 cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttggtgtt
     9841 caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag agcacgtccg
     9901 tgctgtgtcc tttcaggctc tcttcaatga aactccccga ctctaaatga agcgtgttga
     9961 ggtcacaaag ttctcgtaat tccactggca aatgtatctc cagaaagtcg cgagccgtct
    10021 ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca
    10081 tcttccctgt cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgcgccgtcg
    10141 tcagccgcct ctttagtgtt taccgagcag cgttcagaga aatttcaatt cgttacaggc
    10201 gatacatggc acgcttcggc gcggtacgca aactgctgac gcaaatgcca tcagatgagt
    10261 agacactcat caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcaccgcct
    10321 gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa
    10381 actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga
    10441 ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccatcgata tctggaatgt
    10501 caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat
    10561 ttctgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag
    10621 cttcgttacc atgccagatg aagaagctcg cacctggcga cccaatgtcg cctattttga
    10681 aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt
    10741 atccctgcgg ctggttactc atcagccgcg acatcgtctc cagcgaatcc gttcttaaga
    10801 tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg
    10861 tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc
    10921 caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac
    10981 gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgatagc
    11041 catccacccg atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg cgaatttcgt
    11101 taagctcaga aacgctcata aattgcagcg attttgggtt ccacttctgc gggtgacgtt
    11161 tgatgcgcga ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc aatacaggat
    11221 acgcatagcg gctcaccgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag
    11281 ggagattgat cttattcttc acgtaacctt ccagctgcgc catgctcagt gtcgcgtatc
    11341 ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag
    11401 tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg gtgataggtt agcaccggca
    11461 ggccattatc gggttgggca tccagcgcgc tgatataggc gagtcgatcg ccaatacgga
    11521 tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat
    11581 tgtccgccag taccccaaat ggcgcactgc ccgcactcgg cgcgttatag accagcgtat
    11641 ctttccaggt aattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt
    11701 caactttttg tcgcccctga actggctcga gatgcccttt atcgataaac cctttaccaa
    11761 agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg
    11821 cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagatc gcagcatttt
    11881 cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca
    11941 gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt
    12001 attgtagcaa aagcgctata agaatcgtgg gttaatactc atacaaatta tgtttgagga
    12061 cgaaaggcgg atttttttgt tgttggtgcc aaagactact cacctccggc ccacccagtt
    12121 cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc atcatctgtt
    12181 caattaacgg taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg tggcttaata
    12241 atgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac
    12301 cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaacc
    12361 cacgccagaa attcacggcc cggcactgaa gaagcgcccc atgttttttc tccagcgcat
    12421 ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc
    12481 tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa
    12541 ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg
    12601 ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa
    12661 ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat
    12721 ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga
    12781 gtaaaagacc ccacccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat
    12841 gtcatcgaac gttttggcaa acacaccgtt cagcaaaccc gcaagggaga acaacaccgc
    12901 cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa
    12961 cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac
    13021 atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg
    13081 ctggaacttg gcaccaaaaa atgatgacgc aacgttggcg taggcattgg tgatcaccga
    13141 catcatgatc agccccggta cgatgaactg catatagctg aagccatgca tatcgccaat
    13201 acgcgaacca atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg
    13261 caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt
    13321 tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa
    13381 acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc
    13441 tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc
    13501 gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt
    13561 gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta
    13621 ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct
    13681 tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag
    13741 ccccacattg agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt
    13801 agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat
    13861 aacatacgcg cacgttcgtt gcgttttccc catagatcga gttgtttaag atatttttcg
    13921 ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt taccacaatt
    13981 tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt
    14041 ttagcgttca cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt
    14101 ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg
    14161 agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg
    14221 ccgcctggat aagttttttt aagctgttga agttccagtg caatggtcat aaatttttac
    14281 ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta
    14341 gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata gatacactca
    14401 tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga
    14461 aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc
    14521 ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta
    14581 acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac
    14641 tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag
    14701 ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt
    14761 tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg
    14821 aactgaacgt catggaacag gtttataacc tgggccactc caccattatg caatcagcgt
    14881 ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc
    14941 tgcgtgatct ggatgtcaca gcgaccaacc gcgaaaccct tgagcaacgt taccgtcacg
    15001 ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca
    15061 acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag
    15121 atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga
    15181 gaaaccgata aattcaaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt
    15241 acaaatcgcc agcgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt
    15301 attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc
    15361 cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc
    15421 ggtcataaag tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat
    15481 aaatgagcca cgcagcagac caaccagcac catatcgctg ccgctgtctt tgtaacgctc
    15541 agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac
    15601 ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg
    15661 caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc
    15721 gcggaatctg ttaa
//
LOCUS       DAPPNJ010000105        15682 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000105 DAPPNJ010000000
VERSION     DAPPNJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.106"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1255)
                     /gene="uidB"
                     /locus_tag="RPM73_002750"
     CDS             complement(<1..1255)
                     /gene="uidB"
                     /locus_tag="RPM73_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDX6521093.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFML"
     gene            complement(1252..3063)
                     /gene="uidA"
                     /locus_tag="RPM73_002751"
     CDS             complement(1252..3063)
                     /gene="uidA"
                     /locus_tag="RPM73_002751"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDX6521094.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3452..4042)
                     /gene="uidR"
                     /locus_tag="RPM73_002752"
     CDS             complement(3452..4042)
                     /gene="uidR"
                     /locus_tag="RPM73_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDX6521095.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4271..5038)
                     /gene="hdhA"
                     /locus_tag="RPM73_002753"
     CDS             complement(4271..5038)
                     /gene="hdhA"
                     /locus_tag="RPM73_002753"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDX6521096.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5150..6178)
                     /gene="malI"
                     /locus_tag="RPM73_002754"
     CDS             complement(5150..6178)
                     /gene="malI"
                     /locus_tag="RPM73_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDX6521097.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6353..7945
                     /gene="malX"
                     /locus_tag="RPM73_002755"
     CDS             6353..7945
                     /gene="malX"
                     /locus_tag="RPM73_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDX6521098.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            7955..9127
                     /gene="malY"
                     /locus_tag="RPM73_002756"
     CDS             7955..9127
                     /gene="malY"
                     /locus_tag="RPM73_002756"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDX6521099.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9231..10232
                     /gene="add"
                     /locus_tag="RPM73_002757"
     CDS             9231..10232
                     /gene="add"
                     /locus_tag="RPM73_002757"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDX6521100.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGHQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10267..11307)
                     /locus_tag="RPM73_002758"
     CDS             complement(10267..11307)
                     /locus_tag="RPM73_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX6521101.1"
                     /translation="MSDNIRVGWIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11550..11675
                     /gene="blr"
                     /locus_tag="RPM73_002759"
     CDS             11550..11675
                     /gene="blr"
                     /locus_tag="RPM73_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDX6521102.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            11948..12163
                     /gene="ydgT"
                     /locus_tag="RPM73_002760"
     CDS             11948..12163
                     /gene="ydgT"
                     /locus_tag="RPM73_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDX6521103.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12249..12689
                     /locus_tag="RPM73_002761"
     CDS             12249..12689
                     /locus_tag="RPM73_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDX6521104.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12766..13347
                     /gene="rsxA"
                     /locus_tag="RPM73_002762"
     CDS             12766..13347
                     /gene="rsxA"
                     /locus_tag="RPM73_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDX6521105.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13347..13925
                     /gene="rsxB"
                     /locus_tag="RPM73_002763"
     CDS             13347..13925
                     /gene="rsxB"
                     /locus_tag="RPM73_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDX6521106.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            13918..>15682
                     /gene="rsxC"
                     /locus_tag="RPM73_002764"
     CDS             13918..>15682
                     /gene="rsxC"
                     /locus_tag="RPM73_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDX6521107.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRK"
BASE COUNT         3727 a         4081 c         3925 g         3949 t
ORIGIN      
        1 gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg gatgcccata ataacttccg
       61 gcgtttgcac ctgattggcg atatatccgc ttaatcccaa aataaaggca ggaattgaac
      121 ctccgattgc ctgaccgcat ttacgggtaa atgagaatag tgaataggtg agcccttcaa
      181 ttcgcacgcc ggtcaggtat tcaccgtatt ctacggtatc agcttccagt gcccacatca
      241 cagtcatagt aacgccctga ccaattgaag cgatggccaa cgcaaccaac gccaccggca
      301 gtgaccagac ggaaacccag aagaatagca gataaccgca ggttcccagc aaagcgccaa
      361 tcaggaaggt attcttttta ccgatcctcg cgaccatccc cggcaccagc ggtgccgatg
      421 ccacagtacc aaccaggttt tgcaccagta ccagcacagt gaacagcccg gtatcattta
      481 acacatagcg cacgtagaac aacgacgagg cgctgaccgc gaaggtcgaa atcagcacac
      541 acagcgcacc gatgcacaac ataaacagcg ggcgattccg tttcagggtt tgcagactga
      601 tcttcaatga cggctgcgcc acgatacgta ccacattctc acgcgtcgat ttgaagcaga
      661 tgaagtaaag caccattccg gcaatcgcca gcacaatcgt ccagaaatgg tataccgaca
      721 ccatctcttc cgggctggag ttcttaatgc tcggtcctat cagaaatgcc aggcagacaa
      781 aggttaatga agcggcaatc ccgcgagccg cgcccagacg ggcgcgggat tgtggttgtt
      841 gggtcatcgc ggtagcaagt gaaccataag gaatattcac caggctgtag caaagcccga
      901 ggcccatgta ggtcaaatat gcatacacca ctttgctacc atggctccag tcggtcggca
      961 cccagaatac cagcacgctg aagatcatta acggcgcagt accgaagagt aaaaacgggc
     1021 ggaattttcc ccagcgggta ttcacactgt ccaccactcg tccggcaaag acgtcggcga
     1081 aggcatcgaa tacccgcacc agtaacagca tggtgcccgc cgcagcggca ccgacgccag
     1141 cgacgtcggt gtagtaactc aacaggaaga gcgcccccat tgcgaaggcg aagttattgg
     1201 cgacgtcacc gaggctgtag ccgacgatgg tgcgccagga gagttgttga ttcattgttt
     1261 gcctccctgc tgcggttttt caccgaagtt catgccagtc cagcgttttt gcagcagaaa
     1321 agccgccgac ttcggtttgc ggtcgcgagt gaagatccct ttcttgttac ctccaacgcg
     1381 caatatgcct tgcgaggtcg caaaatcggc gaaattccat acctgttcac cgacgacggc
     1441 gctgacgcga tcaaagacgc ggtgatacat atccagccat gcacactgat actcttcact
     1501 ccacatgtcg gtgtacattg agtgcagccc ggctaacgta tccacgccgt attcggtgat
     1561 gataatcggc tgatgcagtt tctcctgcca ggccagaagt tctttttcca gtaccttctc
     1621 tgccgtttcc aaatcgccgc tttggacata ccaaccgtaa taacggttaa ggcacagcac
     1681 atcaaagaga tcgctgatgg tatcggtgtg agcgtcgcag aacattacat tgacgcaggt
     1741 gatcggacgc gtcgggtcga gtttacgcgt tgcttccgcc agtggcgcga aatattctcg
     1801 tgcaccttgc ggacgggtat ccggttcgtt ggcaatactc cacatcacca cgcttgggtg
     1861 gtttttgtca cgcgctatca gctctttaat cgcctgtaag tgcgcctgct gagtttcccc
     1921 gttgactgcc tcttcgctgt acagttcttt cggcttgttg cccgcttcga aaccaatgcc
     1981 taaagagagg ttaaagccga cagcagcagt ttcatcaatc accacgatgc catgttcatc
     2041 tgcccagtcg agcatctctt cagcgtaagg gtaatgcgag gtacggtagg agttggcccc
     2101 aatccagtcc attaatgcgt ggtcgtgcac catcagcacg ttatcgaatc ctttgccacg
     2161 caagtccgca tcttcatgac gaccaaagcc agtaaagtag aacggtttgt ggttaatcag
     2221 gaactgttcg cccttcactg ccactgaccg gatgccgacg cgaagcgggt agatatcaca
     2281 ctctgtctgg cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca
     2341 gaggtgcgga ttcaccactt gcaaagtccc gctagtgcct tgtccagttg caaccacctg
     2401 ttgatccgca tcacgcagtt caacgctgac atcaccattg gccaccacct gccagtcaac
     2461 agacgcgtgg ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt
     2521 gttcggcgtg gtgtagagca ttacgctgcg atggatcccg gcatagttaa agaaatcatg
     2581 gaagtaagac tgctttttct tgccgttttc gtcggtaatc accattcccg gcgggatagt
     2641 ctgccagttc agttcgttgt tcacacaaac ggtgatacgt acacttttcc cggcaataac
     2701 atacggcgtg acatcggctt caaatggcgt atagccgccc tgatgctcca tcacttcctg
     2761 attattgacc cacactttgc cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg
     2821 gcctgcccaa cctttcggta taaagacttc gcgctgatac cagacgttgc ccgcataatt
     2881 acgaatatct gcatcggcga actgatcgtt aaaactgcct ggcacagcaa ttgcccggct
     2941 ttcttgtaac gcgctttccc accaacgctg atcaattcca cagttttcgc gatccagact
     3001 gaatgcccac aggccgtcga gttttttgat ttcacgggtt ggggtttcta caggacgtaa
     3061 cataagggac tcctcattaa gataaaaata ctggtcaacc tttaatctga ttagttaacc
     3121 aattctgtgt tgcacacagt tatctgtgag aaacccatgg aagagaaatg gaaataaaaa
     3181 gaactgacac cgcgatcaaa aaaccaaaaa ctgcaatacc cacagcgtga tattgatcgc
     3241 attaatcttt aaaacggcgt tgacagaagc cataaaataa gcaaccgaac gttcgttcga
     3301 gtatattggg aacgataaac tctcatgaca tatggctacg gtgaatattt ttggggggga
     3361 attctccgat cccggctaga ttgtattttg tacagggaaa ttaaagtatg aaacgcgggc
     3421 acagttatca ccagcccgcg aaatgagagg atcaggatgc ggttaagata ccgccaatca
     3481 tcgcgcgtaa tccctgagca aacgacgctt cacgcgccag atcttccgct tcgatatcgg
     3541 ccagtacacc gtaggtcaga tccagcagta aacgtgaagt cattgacgtg ttgatgtctg
     3601 ggcttatctc gcctttttgc tgggcatcac gcatccgctg ggcaacaaat tcagtgatcg
     3661 tcatatgctt atttttaagc atggcggcga cctgtgggtt acgcatccct tccgccataa
     3721 tttcgaccac cagcgcccgt tgcccaaagg cttcatgggt gagagagaca atagactcga
     3781 ccatatagtc aacaaaatga atcccttcaa tcggttcccg gaaacgggcc agcgccctct
     3841 cctggtcctg taagataatc gcctgaatca aggcttcttt ggagatgaaa tggtgataga
     3901 gcgtcccggg actaatggcg caggatttac agatggcttt catcgaggca ctgtgaaatc
     3961 cattttctga aaaaatctct ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt
     4021 cagtctgcat gttatccatc attaaaccat tatagttagc gtgaataact agtttaccgc
     4081 gttgtcaggt gagcgcctat cattgctgtt gaatagtgcg aaggcacact ctattcgtat
     4141 gcttttcatc cagctattgg ttaaccaata gccgtgattt ggctgccagt tgtttagcag
     4201 agacgttgat gaattgcaca cgctatcccg gtaaacaaca catacggctg tttaccggtt
     4261 cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc
     4321 ttacccagct ggctgcaggc gagcaaagga acagcgccgc gttagcaata tcttgcggtt
     4381 ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa
     4441 cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccattcacc cgaatatttt
     4501 tttcacccag gtcaaatgcc atatttctga ccagatgact ggccgcagct ttagatgatg
     4561 cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa
     4621 cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa
     4681 acacattcag ttcataagca cggcgaaaat ctgccattgg catatcaaac ggtttaggac
     4741 caccgccacc ggcgttgtta accaggatat caactttacc cagcttactg acggcaaagt
     4801 ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgac
     4861 cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga
     4921 ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac
     4981 ccgcacctgt gatgatggcg cattttccgt cgagtctcag gttgtcagaa ttaaacacat
     5041 aaacctcctg tgaacttcag taaaaataaa aggcaccgcc tgtgtgccac attttttatc
     5101 attaaccttg aggaaaggac actaccaggt atagcagata aagagaggat tatttcgccg
     5161 caatgagccg ggcgggaata ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt
     5221 gcatcatgcg atccgcaagt gtggtaccaa gttcccgcgc aggcgtgctg gcccaggtaa
     5281 cgggtatatc atcaagtgtg gttggtgtcg catcggtaaa tgccgccagc gaaacttgtt
     5341 gctcaaagta acgatcgact ccgctttccc cgctttgccg ccctgctttg agcaaaccaa
     5401 accatgcccc catcgcaata gtttcgttat agcaaaccac agcactgatg gtcgggttat
     5461 gacgtaaaag cgccgtgata gcttccgcgg cttgcttctg gctggatgtg cactccagca
     5521 cccaatcgct gtgaaacggc aggccaaatt ttagtagagt tgcacaatag ccccccaccc
     5581 gttctgcacg ggttaatgag gaactttgcc ctcccagcca ggcgatccgc tgatgcccat
     5641 tgcgaatgag atgctccgtc aacaactgtg cagcctgcat gttgtccggg cgaaccgtat
     5701 caacatcatc aagataactg gcacgggaag cgaaaatcac cgggatagct ttttcttctg
     5761 ccatccgtcg caggtcatcg ctgcttcctg cagccccggc aattaccaca ccatccacac
     5821 cctgattcag taacagtgaa aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa
     5881 gcaaaaaaac catccgtccc tgcgcttcca gagcttccgt caatccggcc gtcaattcgg
     5941 cgtaaaacgg cgcagataaa tcacggacga tcaaaccaat gacgccgctt tgcccgccgc
     6001 gcagcgccga cgcctggcga ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc
     6061 gttctcctgt ggcggtagag attcgccctt tgccactaag caccagcgaa acggtactta
     6121 ccgacacgcc cgcagccagc gcaacatcat gaatggttat ttttttggcg gtagccatgc
     6181 agatgaccta ctccctgatt atgtgacaga taaaacgttt taccttttat ttatcttata
     6241 cccgctatta tcgttgcgta atgtaattta tgcctcacta aaatttgata aaacgtttta
     6301 tcttctcgcg caatttactg aatccagatt gttctctacg aggagtcgtt ttatgacggc
     6361 gaaaacagca ccgaaagtca cgctgtggga gttcttccag cagttaggca aaaccttcat
     6421 gttacccgtg gcattattgt cgttctgcgg cattatgctc ggcattggta gttctcttag
     6481 cagccatgat gtcataaccc tgatcccggt cctgggcaac cccgtgttgc aggctatctt
     6541 tacctggatg agtaagattg gctcgtttgc ttttagtttc ctgcctgtca tgttctgtat
     6601 cgccatcccg ctgggcctgg cacgcgaaaa taaaggcgta gcggcattcg ctggcttcgt
     6661 cggttatgcg gtaatgaacc tcgcggtaaa cttctggttg accaataaag gcattctgcc
     6721 aaccacggat gccgcggttc tgaaagccaa taacatccag agcattcttg ggatccagtc
     6781 gatcgatacc gggatcctcg gtgcggtgat cgccggtatt atcgtctgga tgctgcatga
     6841 gcgtttccat aatatccgcc tgccggatgc gctggcattc ttcggcggta cgcgcttcgt
     6901 accaattatc tcctcgctgg tgatgggcct tatcggcctg gtgattccat tagtctggcc
     6961 gattttcgcc atgggtatta gcggcttggg ccatatgata aacagcgcgg gtgatttcgg
     7021 accgatgctg tttggtaccg gtgaacgtct gctgttgccg tttggtctgc atcacattct
     7081 ggtggcatta attcgcttta ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt
     7141 cagcggcgca ctgaccatct tccaggcgca attgagttgc ccgaccactc acggtttttc
     7201 tgaaagcgcc acgcgtttcc tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc
     7261 aggtgcagcg ttagctatgt atcactgcgc gcgcccggaa aatcgccata aaattaaagg
     7321 tctgctgatt tctggcctga tcgcctgtgt cgttggcggc acgaccgaac cgctggaatt
     7381 cctgttcctg ttcgtggcgc cagttctgta tgtcatccac gcgctgttaa ccggcctcgg
     7441 cttcactgtc atgtctgtgc tcggcgtcac catcggtaat accgacggca atatcatcga
     7501 cttcgtggtg ttcggtattt tgcatggtct gtcaaccaag tggtacatgg tgccagtggt
     7561 ggcggcaatc tggtttgtcg tttactacgt catcttccgt ttcgctatca cccgcttcaa
     7621 tctgaaaacc ccggggcgcg atagcgaagt tgccagctca atcgaaaaag ccgttgccgg
     7681 tgcgccgggt aaatcaggtt acaacgttcc tgcaatcctc gaagcattag gcggtgccga
     7741 caatattgtc agcctcgata actgcattac ccgtctgcgt ttgtctgtga aagatatgtc
     7801 gcttgttaat gtgcaggcac tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca
     7861 acataacctg caggttgtta tcgggccaca agttcagtca gtaaaagatg aaatggccgg
     7921 tctgatgcat actgtccggg cataaggata agatatgttc gatttttcaa aggtcgtgga
     7981 tcgtcatggc acatggtgta cacagtggga ttatgtcgct gaccgtttcg gcactgctga
     8041 cctgttaccg ttcacgattt cagacatgga ttttgccact gccccctgca ttatcgaggc
     8101 gctgaatcag cgcctgatgc acggcgtatt tggctacagc cgctggaaaa acgatgagtt
     8161 tctcgcggct attgcccact ggttttccac ccagcattac accgcaattg atacgcagac
     8221 ggtggtgtat ggcccttctg tcatctatat ggtttcagaa ctgattcgtc agtggtctga
     8281 aacaggtgaa ggcgtggtga tccacacgcc cgcctatgac gcattttaca aggccattga
     8341 aggtaaccag cgcacagtaa tgcccgttgc tttagagaag caggctgacg actggttttg
     8401 cgatatgggc aagctggaag ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg
     8461 tagcccacag aatcctaccg ggaaagtgtg gacgtgcgat gagctggaga tcatggctga
     8521 cctgtgcgag cgtcatggtg tgcgggttat ttccgatgaa atccatatgg atatggtctg
     8581 gggcgagcag ccgcatattc cctggagtaa tgtggcgcgg ggagactggg cgttgctgac
     8641 gtcgggctcg aaaagtttca atattcccgc gctgaccggt gcttacggga ttatagaaaa
     8701 tagcagcagc cgtgatgcct atttatcggc actgaaagga cgtgatgggc tttcttcccc
     8761 ttcggtactg gcgttaactg cccatatcgc cgcctatcag caaggtgcac cgtggctgga
     8821 tgccttacgc gtctatctga aagataacct gacgtatatc tcagataaaa tgaacgccgc
     8881 gtttcctgaa ctcaactggc agatcccgca atccacttat ctggcctggc ttgatctacg
     8941 tccgttgaat attgacgaca acgcgttgca aaaagcgctt atcgagcaag aaaaagtcgc
     9001 gatcatgccg gggtatacct atggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg
     9061 ctgcccacgt tcgaaactgg aaaaaggtgt ggctggatta ataaacgcca tccgcgctgt
     9121 tcgctaaccc caattgcgca acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc
     9181 tttgttttta taatggtgcg cacttttata ttcagaaaaa gagtgcgacc atgattgata
     9241 ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt ccccagacca
     9301 ttcttgaact tggccaccag tataatatct cgcttcctgc acaatccctg gaaacactga
     9361 ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg accaaacttg
     9421 actggggcgt taaagttctc gcctctcttg atgcctgtcg ccgcgtggca tttgaaaaca
     9481 ttgaagatgc agcccgtcac ggcctgcact atgtcgagct gcgtttttca ccaggctaca
     9541 tggcaatggc acatcagctg cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac
     9601 gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg agccggacct
     9661 tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt gaccagatta
     9721 ccgcacttga tttagccggt gatgaacttg gtttcccggg aagtctgttc ctttctcact
     9781 tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc
     9841 cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga catggcgtaa
     9901 aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt ggtattgaat
     9961 cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca catccgctga
    10021 aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc ggcgtacagg
    10081 gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta tcccgcgagc
    10141 aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgct gaggaaaaac
    10201 gcgcactgcg agaaaaagtc gccgcgaagt aacaaaatgg atggtgcaaa ttgcaccatc
    10261 catttttcat gcaaggcaca aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat
    10321 caactccatt acctggattg cctggcttgc cggaaccgga ttttcaccat cgccatttaa
    10381 cgcatcacga atagccgcat agtaagccgg ataattccca ggcaccgtca acagcgtttc
    10441 ttcgacacgt tcctcacctt ccacgcgggt aagtacgcca tcacgcatat cgtagcccca
    10501 gtcttcctgc ggtagacgct cgccattttt cagacgttct tcctgtggat cgaggccata
    10561 tttcacataa ctgcctcggg atccatgcac gatataccgt gctgactcag cggctgccag
    10621 catggtaccg tgtaaaatga ctcgccgctg tggataggac aagatggcgt ggaaataatc
    10681 ggtcgactgc gctccgggcc gtaactgtgc caaatctacc gtcatgctga ccggtaaacc
    10741 aaaaagcgta atggcctgat caagaagatg tggtgctaaa tcgtaccaga taccgctgcc
    10801 tgggccgccc tgttcacgcc aacgatcgcg cacctgcgga cggaagcggt caaaatgaga
    10861 ctcaaagtaa gcaacttcac ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa
    10921 atcgctatcc cagcgacggt tatggaatac agacagcaca cgccccaggc tttttgccag
    10981 tgcatccagc tctcgcgctt gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg
    11041 tttacccgcc tcaagcgccg ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat
    11101 gacaatcagg tctatgttgg gatcgttaaa cagatgcttc ggctcagaga caaccgtaac
    11161 cgttggccag tcggctttta cttttgtttc atcactgctg gagattaccg ccagttccag
    11221 cccgggcgtg cccgcaatca ggggcgcatg gaaggttttg ctcgcataac catacccaat
    11281 ccacccaaca cggatgttgt cgctcatgtc attgcctctc attgaagtat gatggctatt
    11341 tgacactatc ctttacccac gctcaacagt ttaataacct gccagcaata agggatgttg
    11401 tttaacttaa gtcaaaaaaa tagcgaattt tccaacgaca aaagctaaat atcgcaaaaa
    11461 cctcagtaaa aatcttgctg gagctattat tgctaagtaa catttacccc ctgaagttaa
    11521 tggatcaatc aagagagatg tgggctgtaa tgaatcgtct tattgaatta acaggttgga
    11581 tcgttcttgt cgtttcagtc attcttcttg gcgtggcgag tcacattgac aactatcagc
    11641 cacctgaaca gagtgcttcg gtacaacaca agtaagctct gcacttgtgg agcgacatgc
    11701 tgcccgtccg ggtgcatgtt ttcacttgtc ggatattaaa ccaggaattt attatcttgt
    11761 tcgatgttgt tggtgattgt cagggatagt aaagcgcgtt actcttttca gagcagtttt
    11821 tcatccctga atatttgcca tcagcgcatt cttattattg taataacaca agcgttcact
    11881 tttggtgaaa ttttatcatc actaataata cctacccctt tggtattaat tattttatgg
    11941 accttttatg actgttcagg actacttatt aaaatttcgc aaaatcagtt cactcgaaag
    12001 tctggaaaaa ctctacgacc atcttaatta caccctgacg gacgatcagg aactgatcaa
    12061 tatgtatcgt gctgccgatc accgtcgcgc agagctggtt tctggcgggc gtttgtttga
    12121 cctcggccag gtaccgaagt ccgtctggca ctatgtccaa taaagaaagt agcgatcggt
    12181 tataaaagcc ttataataac gcccctaaaa ttagatttgc ctgtttgcgt cgctattctg
    12241 gagaacctat gaccaccaca acgccacaac gtattggagg ctggttgctt ggcccgttgg
    12301 cctggctttt agtcgctctg ttgagtacga cgctggcgtt gttgctgtat accgctgcgt
    12361 tatcttctcc gcaaacattt caaacgctcg gcggacaagc actgacgaca caaatcttat
    12421 ggggcgtttc ttttattacc gctatcgcca tgtggtatta cacactgtgg ttgaccatcg
    12481 ctttctttaa acgtcggcgt tgtgttccta aacactatat tatctggctg cttatttccg
    12541 tattgctggc agtaaaagca ttcgcttttt cacccgtgga agatggcata gccgttcgcc
    12601 agttgctgtt tactttgttg gcaaccgcat tgatcgtccc ctatttcaaa cgctcgtcgc
    12661 gggtaaaagc gacgtttgtg aatccgtaat aaccttacag ttaacctgtt gtcgcctgct
    12721 ctggattaac ggataatagg cggctttttt atttcaggcc gaaaaatgac tgactacctg
    12781 ttactgtttg tcggaactgt actggtcaat aactttgtac tggtcaagtt tctcggtctc
    12841 tgtccgttta tgggggtttc caaaaaactg gaaaccgcga tgggcatggg gctggcaaca
    12901 acgtttgtga tgacgctggc gtctatttgc gcctggctta tcgatacgtg gattttgatc
    12961 ccacttaatc tgatttacct gcgcaccctg gcatttattc tggtgattgc tgtggtcgtg
    13021 cagttcaccg agatggtggt gcgcaaaacc agcccggtgc tttaccgtct gctggggatt
    13081 tttttgccgc ttatcaccac caactgtgca gtgctcggcg tggcgttgct gaatatcaat
    13141 ctcgggcaca atttcttgca gtcggcgctg tacggttttt ccgccgctgt cggtttctcg
    13201 ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg ctgtggctga tgtcccggca
    13261 ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc ttatgtctct ggcctttatg
    13321 ggctttagtg gtttggtgaa gttgtaatga atgctatctg gattgccgtt gccgccgtga
    13381 gcctgctggg cctggcgttt ggcgccattc tgggttatgc ctcccgccgt tttgcggtgg
    13441 aagacgatcc ggtcgttgag aaaattgacg aaatcttacc gcagagccag tgtggtcagt
    13501 gcggttatcc cggctgtcgc ccctacgcgg aagccatcag ctgtaacggt gaaaaaatca
    13561 accgttgcgc cccaggtggc gaagctgtga tgctaaaaat tgccgagttg cttaatgtcg
    13621 agccgcagcc gctggatggc gaagcgcaag agataacgcc tgcgcggatg gtggcggtta
    13681 ttgatgaaaa taactgtatt ggctgcacta aatgtattca ggcgtgtccg gtagacgcca
    13741 tcgttggcgc tacccgtgcc atgcatacgg taatgagtga tctctgtacg ggctgcaatt
    13801 tatgtgttga tccgtgcccg acgcactgca tctcgttgca accggtcgca gaaacacctg
    13861 actcctggaa atgggatctg aacaccattc ccgtgcgtat cattcccgtg gaacaccatg
    13921 cttaagttat tctctgcatt cagaaaaaat aaaatctggg atttcaacgg cggcatccat
    13981 ccaccggaga tgaaaaccca gtccaacggt acacccctgc gccaggtacc actggcgcag
    14041 cgttttgtta ttccactgaa acagcatatt ggcgctgaag gtgagttgtg cgttagcgtc
    14101 ggcgataaag tattgcgcgg ccagccgctt acccgtggtc gcggcaaaat gctgcctgtt
    14161 cacgcgccca cctcgggtac cgttacggct attgcgcccc actctacggc tcatccttca
    14221 gctttagctg aattaagcgt gattattgat gccgatggtg aagactgctg gatcccgcgc
    14281 gacggctggg ccgattatcg cactcgcagt cgcgaagagt taatcgagcg catacatcag
    14341 tttggtgttg ccgggctggg cggtgcagga ttcccgacag gcgttaaatt gcagggtggc
    14401 ggagataaga ttgaaacgtt gattatcaac gcggctgagt gcgagccgta cattaccgcc
    14461 gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag agggtattcg cattcttgcg
    14521 catattctgc agccacgcga aattcttatc ggcattgaag ataacaaacc gcaggcgatt
    14581 tccatgctgc gcgcggtgct ggcggactct aacgatattt ctctgcgggt gattccaacc
    14641 aaatatcctt ctggcggtgc taaacaatta acctacattc tgaccgggaa gcaggttcca
    14701 catggcgggc gttcatccga tatcggcgta ttaatgcaaa acgtcggcac tgcttatgca
    14761 gtgaaacgtg ccgttattga tggcgagccg attaccgagc gtgttgtaac cctgactggc
    14821 gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg ggacgccagt gcgtcattta
    14881 ttgaatgatg ccggattctg cccctctgcc gatcaaatgg tgattatggg tggcccgcta
    14941 atgggcttta ccttgccatg gctggatgtc ccggtcgtaa agattaccaa ctgtctgttg
    15001 gctccctctg ccaatgaact tggcgaacca caggaagaac aaagctgcat ccggtgtagc
    15061 gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac agttgtactg gttcagcaaa
    15121 ggtcagcaac acgataaagc taccacgcat aacattgctg attgcattga atgtggggct
    15181 tgcgcgtggg tttgcccgag caatattccc ctggtgcaat atttccgtca ggaaaaagct
    15241 gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag aagccaaagc gcgtttcgaa
    15301 gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc ttgaacgaca taagagcgca
    15361 gccgttcaac ctgcagccaa agataaagat gcgattgctg ccgctctggc gcgggtgaaa
    15421 gagaaacagg cccaggctac acagcctatt gtgattaaag cgggcgaacg cccggataac
    15481 agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag ccagagcgaa acaggcagaa
    15541 ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac gtaaaactgc cgttgaagca
    15601 gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac aggctaatgc ggaaccagaa
    15661 gaacaggtcg atccgcgcaa ag
//
LOCUS       DAPPNJ010000106        15638 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000106 DAPPNJ010000000
VERSION     DAPPNJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.107"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(442..1248)
                     /gene="trpA"
                     /locus_tag="RPM73_002765"
     CDS             complement(442..1248)
                     /gene="trpA"
                     /locus_tag="RPM73_002765"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDX6521108.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1248..2441)
                     /gene="trpB"
                     /locus_tag="RPM73_002766"
     CDS             complement(1248..2441)
                     /gene="trpB"
                     /locus_tag="RPM73_002766"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDX6521109.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2453..3814)
                     /gene="trpCF"
                     /locus_tag="RPM73_002767"
     CDS             complement(2453..3814)
                     /gene="trpCF"
                     /locus_tag="RPM73_002767"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDX6521110.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3815..5410)
                     /gene="trpD"
                     /locus_tag="RPM73_002768"
     CDS             complement(3815..5410)
                     /gene="trpD"
                     /locus_tag="RPM73_002768"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDX6521111.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5410..6972)
                     /gene="trpE"
                     /locus_tag="RPM73_002769"
     CDS             complement(5410..6972)
                     /gene="trpE"
                     /locus_tag="RPM73_002769"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDX6521112.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6994..7087)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7064..7108)
                     /gene="trpL"
                     /locus_tag="RPM73_002770"
     CDS             complement(7064..7108)
                     /gene="trpL"
                     /locus_tag="RPM73_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDX6521113.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7246..8127
                     /gene="yciV"
                     /locus_tag="RPM73_002771"
     CDS             7246..8127
                     /gene="yciV"
                     /locus_tag="RPM73_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDX6521114.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8124..8744
                     /locus_tag="RPM73_002772"
     CDS             8124..8744
                     /locus_tag="RPM73_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDX6521115.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8845..9717
                     /gene="rluB"
                     /locus_tag="RPM73_002773"
     CDS             8845..9717
                     /gene="rluB"
                     /locus_tag="RPM73_002773"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDX6521116.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9757..10347)
                     /gene="cobO"
                     /locus_tag="RPM73_002774"
     CDS             complement(9757..10347)
                     /gene="cobO"
                     /locus_tag="RPM73_002774"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDX6521117.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(10344..11102)
                     /locus_tag="RPM73_002775"
     CDS             complement(10344..11102)
                     /locus_tag="RPM73_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDX6521118.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11322..12371
                     /gene="sohB"
                     /locus_tag="RPM73_002776"
     CDS             11322..12371
                     /gene="sohB"
                     /locus_tag="RPM73_002776"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDX6521119.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12407..12658)
                     /locus_tag="RPM73_002777"
     CDS             complement(12407..12658)
                     /locus_tag="RPM73_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDX6521120.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13038..15635
                     /gene="topA"
                     /locus_tag="RPM73_002778"
     CDS             13038..15635
                     /gene="topA"
                     /locus_tag="RPM73_002778"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDX6521121.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3808 a         3956 c         4186 g         3688 t
ORIGIN      
        1 cgtcggatgc cttctcacgg tcttcggcga aatttcctga accaccacga tgttcggcca
       61 tgttatttct cccgttgcgt tgcattgttt cattaatatg agtgttgtgt gtcgacactc
      121 attaaaatta gtcgctaatg agaattagtc aaattaagcg caacgagaag atagagggaa
      181 aatatatttt gaggaacatt ctggatatat taacaattac ctgaggaata agtgacttag
      241 agaaaattat tgattttact ggtgttatgt tgcgggatta atttgtttga ttatattact
      301 gttgggcgga aaaatgacgt aagttgacgt tcgaccgggg taagcgaaac ggtaaaaaga
      361 taaatattaa atgaatttag gatttttccg gcttcattaa agaaagttaa aatgccgcca
      421 gcggaactgg cggctgtggg attaactgcg cgtcgccgct ttcatcggtt gtacaaaaac
      481 tttcagtgcc gccagcattt tctctggctc attaatatgt tgctcgatga ttttaacaat
      541 ggccgaacca gaaatcgcgc ccgcagctcc tgcatcaatc gctgctttta cctgatccgg
      601 ggcggaaata ccaaacccct gcaatggagg tgcagcgttg tactctttca gcttcgcaac
      661 cagatgatta aggggtaacg cggcgcggtt ttctgcgccg gtcacgcccg ctcgtgacag
      721 caaataggtg taaccacgac cgtaagaggc tatctggcgc agcaggtcgt catcggcatt
      781 tggcgggcag atgaagatag gtgcgacatt atggcgcaac gcggcctggc ggaagggcgc
      841 ggactcttca attggcacat cggcaaccag caccgaatcg acgccgactt tctcgcactg
      901 ggcataaaac tcatcaatgc ctttgttaaa caccagattg gcatacatca acaggccaat
      961 gggaatggtc gggtgtttct ggcgaatcag tgccagcatt tcaaaacatt gtgccggagt
     1021 cacacctgcc gcaaaggcgc gcagagtggc gttttgaatc gtcgggccat ccgccagtgg
     1081 gtcggagaag gggataccta actccagcgc gtcagcaccg gcttcaatta gcgtatcgat
     1141 aattttcaat gactgctcaa tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc
     1201 ttctttgcgc tccttcaact gggcaaacag agattcgtag cgttccatca gatttcccct
     1261 cgtgctttca aaatatcgtg aacggtgaag atgtctttat cgccgcgacc ggaaaggtta
     1321 accaccagta gctgctcttt atccgggttt tcgcgcatca ttttcaacgc atgggccagg
     1381 gcgtgggagg attccagcgc cgggatgatc ccttcgtgca ggcacagcgt tttgaaggct
     1441 tcaagggctt catcatcggt aatagacacg taatcagcgc gtccagtgct gttaagatac
     1501 gcgtgttgtg ggccgacaga cgggaaatcc agtccggcgg agatggagta agattcttca
     1561 atctgcccgt cttcggtttg catcatcggc gctttcatac cgaaatagat gcccacgcga
     1621 ccatgtttta acggtgcgcc gtgctcgcca gtttcgatac cgtgaccacc tggctccaca
     1681 ccaatcaggc cgacgttggt ttcattgatg aaatcagcaa acatgccgat ggcattcgaa
     1741 ccgccgccaa cacaggcgat aacggcatcc ggcaggcgac cttctctttc cagaatctgc
     1801 gctttggttt cttcgccaat catccgctga aactcacgca caatggtcgg ataaggatgc
     1861 gggccagctg cggtgcccag catatagtgc gcggtttcgt aactaccgga ccagtcgcgc
     1921 agcgcctcgt tacaggcatc tttcagcgtc gcggaaccgc tatgcaccgg gatcacttcc
     1981 gcacccatta agcgcatacg aaaaacgtta ggcgactggc gttctacgtc tttggcaccc
     2041 atataaatac ggcatttcag gccgagcagg gcgctggcaa gggccgacgc cacgccatgc
     2101 tgaccggcac cggtttcggc gatgatttcg gttttaccca tccgcttcgc cagtaaagcc
     2161 tgaccgagca cctggttagt tttatgcgcg ccgccgtgca gcaaatcttc acgcttgaga
     2221 tacagagtgg tgttcgtccc ggctgtaatg ttctggcatt tggtcagcgc ggttggacgc
     2281 ccggcatagt ttttcagcag gtcgttgaac tgagcctgaa attcaggatc tttttgcgca
     2341 ctgacaaaag cttcttccag ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg
     2401 ccgccaaact caccaaaata ggggttaagt aatgttgtca ttgttccttt ccttaatatg
     2461 cgcgcagcgt ctggaaaacc gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact
     2521 ctacagcaga attaaaatca agtccggcgc agccggtttg tgccgcttcc acgcagttat
     2581 ctgcgcctaa gccccccgcc agcagaacgt tgccaagcga ttgaccattt aatagtgacc
     2641 agtcgaaacg ttgcccgctt ccaccctggc cgttgtctaa aacatattta tcaacgtgct
     2701 gaagctcgcg ggcgggcagg gtttcaccga cgcttaatgc tttccagatg gcaacgtgtg
     2761 ctggcagagc ttcacgcagc gtatcgatat acagctgatc ttcattacca tgcagttgca
     2821 ctgccgccag cgataacacc ttagctttgt ccaccacatc ggcaatatcg tgattgcgga
     2881 acacgccaac atactgcaac ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga
     2941 cgcaacgcgg tgatgtcgca acaaaaatca acccaccgta aatcgcgccc gcgtcataag
     3001 ctgctttagc atcttgccca cgcgtcaggc cacatacttt attctcaccc agcaacaccc
     3061 ggcgcacggc ggcgtgcaaa tcgtcatggg ccatcaacgc cgaaccaatc agaaaaccgt
     3121 tagcgaagtg gcttaactcg cgcacctgag cgtaagtatt gatgccggat tcgctgatta
     3181 ccgttacgtt gtgccccagt ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca
     3241 aatcccgcag atcgcggttg ttgatgccaa cgacctttgc ccccaatgca atggcacgct
     3301 ccagttcctc ttcattactg acttcggtca gcacacccat ctccagactg tgggcgacgg
     3361 cggcaagctg gcgatattgt tcgtcatcca gtactgaaag cattaataag caggcatcag
     3421 cctggtaata gcgcgccaga tagatctggt aaggatcgat aatgaagtct ttacataaaa
     3481 tcggctgcgg ggcgatttgg ctgacgatgg ggaggaaatc aaagctcccc tgaaaatatt
     3541 tctcatcagt cagcaccgaa attgccgaag cgtaatgttt ataaatggcg gcaatgcgtg
     3601 ctggatcgaa atcatcacgg atcacgcctt ttgacggcga cgctttcttg cactccagaa
     3661 taaacgccgt gcgcgcaccc tgtagcgcat cataaaaatg tcgcgtgctc ggctgaacct
     3721 cattctgaaa actggccagc ggttgctgct gtttgcgggt ttctacccaa atcgccttgt
     3781 ctgcgacgat tttcgctaaa acggtttgca tcatttaccc tcgtgccgcc agcgcggtaa
     3841 ctctgtcgta agcggaacca ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca
     3901 gatcttcatg gccatgcagg cgcattaaca tggcgacgtt cgcagcgacg gctgcttcat
     3961 gggcggcgtc gcctttacct tgtaacaaac gtgttaaaat gtcacggttt tcttccggtg
     4021 ttccgcctgc cagttgctcc tggtggtagg gtgtcaggcc aaaatcttca gcggtcaatt
     4081 gatagctctt aatttcgccg tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa
     4141 cttcatccat cccgccgctg tgcaccaccg ccgcgcgttg ataccccagc acgcgcaagg
     4201 tttcggcaat cggcagcacc agttccggac tataaacacc aattaacgcc agcggcggat
     4261 gcgccgggtt aatcaatggc cccagcacat tgaacagggt gcgggttttt agttgctggc
     4321 gaaccggcat tgcatggcga aatccggtgt gatatttcgg tgcaaagagg aaacatacgc
     4381 ctaactcatc cagcgcctgg cgcgatttat cggcgttcat atcaagatta ataccgaacg
     4441 ccgccagcag atcggacgaa ccagatttac tggagacgct acggttgccg tgtttcgcca
     4501 ctttcagccc acaggccgcg gcgacaaacg cactggcggt agaaatattg atactgttgc
     4561 tgccgtcacc gccagtaccg acgatatcag caaacagata atccgggcgc gggaacggcg
     4621 ctgcgttttc cagtagcgcg gttgctgccc cggcgatctc gttcgggtgc tcaccgcgaa
     4681 ttttcatgct caccagcgcc gccgccagtt gttccggctt cagctcgcca cgcaccaccg
     4741 ctgaaaacag ctggtggctt tcttgttggc taagcgtctg cgcctgatac agtttttcca
     4801 gaatcggttg cagcgtgttg gctggctcta gtttctgctg cgcccaggcc agcgtttgtt
     4861 ccagcaggcg agcgccctgg gtggtgagaa tggattccgg atggaactgg aatccacaaa
     4921 cgcgatccgc atcgtgacgt actgccatca ccatgccatt aaaatgggcg ttgatggtta
     4981 aaccggccgg aatgttactg ccaaccagcg agtgataacg cgccaccggc agcgggtttg
     5041 ttaatccggc aaacatcgcc tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa
     5101 tttcgcccgc ctgaccgaca tagcccccgt aagcttcgac aattgcctga tgtccgaggc
     5161 aaatgccaat aatgggcagc ttgccacgca agcgggtgag gagttccggc atacaaccgg
     5221 cttcgctcgg cacaccgggg ccaggagaaa gcatcagtac cggattgctc atggtcgcca
     5281 ggcgttcaat taaggtctgc gccggaatat ggttgcggta aatcaccacg ttatgcccat
     5341 tgctgcgcaa ctgatctgcc aggttgtacg taaaagagtc gatattatcg agcagcagta
     5401 tgtcagccat cagaaagtct cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc
     5461 gcgggcttta ttacgggttt cgtcggcttc cgactgcgga atagaatcaa ggactacgcc
     5521 agcaccggct tgcacggtgg cgataccgtt ttccaccagc gccgagcgga tcacaatgca
     5581 ggtgtcgaga tcgccgtgcg cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg
     5641 acgaccttcc gcctcggcaa ttaactgcat ggcgcgcact ttcggcgcac cgcttaacgt
     5701 ccccatattc atacaggcgc ggtaagcgtg tagggcgtcg agatcgtggc gcagctcacc
     5761 gaccacgcgg gagactaggt gcatcacgta agagtaacgg tcaactttgg tgaggtcggc
     5821 gacgtagcgg ctgccggggg tgcaaatgcg tgccagatca ttacgggcaa gatccaccag
     5881 catcagatgt tcagaaagct ctttatgatc ggtacgcatt tccagttcga tgcggctgtc
     5941 gaggtctctg tccagcgagc catcggcacg acgaccgcgt ggacgtgttc cggcaatcgg
     6001 gtagatctca atctggcggc tggtggcgtc atacttgagc gaactttccg gcgacgcgcc
     6061 aaacagggtg aaatcattat cctgcataaa aaacatgtac gggctgggat tactcttttt
     6121 cagcacgtaa taggccgcca gcggtgacgg gcagggcaga gagaaacggc gagatggcac
     6181 cacctggaaa atttctccag cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa
     6241 ctcttcatcg ctctgattac attcacaacg catatgcggc acggaaacca ccggcagcgg
     6301 cggcgcggct tcggtcagtt gctgacgtag ttcgttcagg cgagcagtga gacgttgttt
     6361 ttcttcttca ttcggagcaa acaggctggc ctgaatacgg gtgctttttt tctgatggtc
     6421 aatcaccatc agagtttcag cgagataaaa acagaaatca gggcagttat tttccgctga
     6481 cagttgcggt aaatcttcaa atcccgccac aaggtcataa gagaacaggc cgccgaagaa
     6541 cattgcttct cgttcttcct tcggtacatt caacagattc tgcaataaac ggaaagcgtc
     6601 aaaaaccgaa agggagcata agcgagcgtc ttcatccagc agtggactga caggggggaa
     6661 gcgcagcaca cggcagtttg gtaattgttc attttccaca cccgcaggca gggcgttatc
     6721 cagtagtgtc agcagggctt cgccgttgcc ggaaagtgcc tggattgtga cagtgtcacc
     6781 taaagctgta atgcgcagcg cactgtctac cagcagcagg ctttttaaat catctttgct
     6841 gtcgatatct gcggattcca gcagcagcgt tgccggacga tccccacaca actggtgaaa
     6901 aagcgcggtg ggattgtcgc gataagcgcc ttcgcaggtt agcagttcga gagtcggttt
     6961 ttgtgtttgc attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc
     7021 tgggtatctg attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc
     7081 aacctttcag tacgaaaatt gctttcattg tcgataccct ttttacgtga acttgcgtac
     7141 tagttaacta gttcgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg
     7201 ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat
     7261 tacgcagtga tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca
     7321 gaagcattgg tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat
     7381 gacaccacag ctgcaatcgc gccagcaaga gaggaaattt cacgttcggg actggcgctg
     7441 aatcttattc ccggcgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc
     7501 gggctgaata ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa
     7561 cggcgcaatc agcgggcgca gctgattgcc gaaagacttg aaaaagcgca aatacctggg
     7621 gcgctggaag gcgcacaacg actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg
     7681 cgtttccttg ttgagtgcgg caaagccagt tcaatggcgg atgtctttaa aaagtatctg
     7741 gcgcgcggga aaaccggata cgttccgcca cagtggtgta caatagaaca agctattgat
     7801 gtcattcatc attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt
     7861 gctaaatggc tgaaaagatt ggttgcgcat tttgccgaac accacggtga cgcgatggaa
     7921 gtcgcgcagt gccagcaatc gcccaatgaa cgtacccagc tggcggccct tgcgcgtcag
     7981 catcatttat gggcatcaca aggatctgat tttcatcagc catgcccgtg gatcgaactg
     8041 ggtcgtaaac tctggctgcc cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg
     8101 cagaacacca cagagaggga attatgagcc agttttttta tattcatcct gataacccac
     8161 agcaacgtct gatcaaccag gcggtggaga tcgtgcgtaa aggtggggtg attgtttatc
     8221 caactgattc cggctatgcg ctcggctgta aaattgaaga taaaaacgcg atggagcgta
     8281 tttgtcgtat tcgccagctg ccggacggtc acaactttac cctgatgtgt cgcgatcttt
     8341 ctgaactctc gacctattca tttgttgata acgttgcgtt tcgtttaatg aaaaacaaca
     8401 cgccgggcaa ctataccttc atcttgaagg ggacgaagga agtgccacgc cgtctgttgc
     8461 aggaaaaacg caaaaccatc ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc
     8521 ttgaagcact gggcgaaccg atgctttcca cttcgctaat gctgccaggc agcgaattta
     8581 ccgaatccga tcccgaagaa attaaagacc gtctggaaaa acaggtggat ttgattatcc
     8641 acggcggcta tctcggacag aaaccgacaa cggttattga tcttaccgat gatacgccgg
     8701 tcgtggtgcg tgaaggcgta ggtgatgtga agcctttctt ataagtgcgt aactctgtat
     8761 actacgcggc cttaaaatgg cgctatacgg cgccattgat tctcctgttc gacgcctgtg
     8821 aaggcgacac caaaaggaag ctctatgagc gaaaaattac agaaagtgct ggcccgcgct
     8881 ggccacggct cccgccgtga aatcgaatct attattgaag ccggtcgtgt gagtgttgat
     8941 ggcaaaattg ctaaactcgg cgatcgcgtt gaagttaccc ctgggctgaa aattcgtatc
     9001 gatggtcatc tgatttcggt acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat
     9061 tacaaaccgg aaggtgagtt gtgtacccgt aacgatccgg aagggcgtcc gacggtgttt
     9121 gaccgtctgc caaagctgcg tggcgcacgc tggattgccg taggtcgtct ggacgttaac
     9181 acctgtggtc tgctgctgtt caccaccgat ggtgaactgg caaaccgttt aatgcatcca
     9241 agccgtgaag ttgaacgtga atacgccgtg cgtgtatttg gtcaggttga cgacgcgaaa
     9301 ctgcgtgatt tgagtcgtgg cgtgcaactg gaagatggtc cggcagcttt taaaaccatc
     9361 aagttcagcg gcggcgaagg gatcaaccag tggtacaacg tgactctgac cgaaggccgt
     9421 aaccgtgaag ttcgtcgtct gtgggaagcg gttggtgtgc aggtgagccg cctgatccgt
     9481 gttcgttacg gtgatatccc actgccaaaa ggtctgccac gcggtggctg gacggagctg
     9541 gacctcgccc agactaacta tctgcgcgaa ctggtggagc ttccgccaga aaccagctct
     9601 aaagtcgctg tagaaaaaga ccgtcgtcgc atgaaggcta atcagattcg tcgtgcggtg
     9661 aaacgtcaca gtcaggtgag cggcggccgt cgttctggcg ggcgtaataa cggttaatca
     9721 aagtatgccg gacgtcatat ccggcatttt tacagattaa taatcaatcc ctatctgcgc
     9781 ttttacaccg gcatcaaacg catgtttgac ggggcgtaat tcacttaccg tatctgccag
     9841 ttcaagaata tcccgatgac aaccacgacc cgtgataatc accgtctgtt gatgtggacg
     9901 ttcatttaac gcctgcacca cttcttccag tggcaaatag tcatacgcca ccatatacgt
     9961 cagttcatca agcaaaacca tatccagtga ggaatcagca agcatccgct ttgcatgttg
    10021 ccagacttcg cggcaggcgg cggtatcaga ctcgcggttt tgtgtatccc aggtaaagcc
    10081 cgttgccatc acctgaaact caacgccatg tggctccagc agattgcgtt cgccattagg
    10141 ccaggtgcct ttaataaact gcacgacgcc tacttttttt ccgtgaccaa ctgcgcgtgt
    10201 tgccgtacca aatgccgcgg tggttttgcc ttttccattg ccggtaaaga cgatgataat
    10261 accgcgttca tcctgggcct gggccacacg agcatctact ttttctttca ctcgctgctg
    10321 acgctgttgg tagcgttcat cactcattgg gaaattcctg gtttacggcc cggttgggcg
    10381 tcaaaggtca tgccggtttt acggcggctg tcatcgccca tcagccagag gtagagcggc
    10441 atgatatcag cgggtgtttt aagtttctgt ggatcttcgg tcgggaaggc gctggcacgc
    10501 attgcggtgc gcgtaccgcc tgggttaatg cagttgacgc gcaggcgctg ctgatattca
    10561 tcggccagta cctgcatcat cccttcggtg gcaaatttcg acgctgcata tgcgccccag
    10621 ttggctcgtc cctgacgtcc aacgcttgat gaagtaaaga ccagtgaacc ggcgtccgat
    10681 ttgagtaata aaggaagcag tgcctgggtg agcataaagg tggcattaac gttgacctgt
    10741 atgacgtcct gccagacctg tggattttgt tcgctcattg ggcaaacatc gccgagcaat
    10801 ccggcattat gcaaaacacc atccagacgc ggataattaa cggtaatgcg ctgtgccagt
    10861 tgttggcaat cttcggacgt gcaggtcagc aaatcgagaa taaaccactg tggctgacgt
    10921 ccagtttctt cgtttatgtg gctggctacc tgacgtaatt tttcttcatt acggcccaac
    10981 agaatcactg tcgcaccata gcgtgcatac gtcatcgcgg cttcacggcc aatcccatcg
    11041 ctggctcccg tcaccaggat aatgcgatca ttgagtaaat cttgttttgg ctggtaatgc
    11101 atggctactc ctcaacgacg ttgtctgtcg cgctgccagt gtactttatg actttggggc
    11161 tttatgcctg aaacagaagg gcatttcaat cagcctgggg cgataacacc gcaaaatgga
    11221 tactttgtca tactttcgct gcaataacat ctctgcgaga cggcttaaca tgcctgttgt
    11281 aaactgtgag ccaaagcgtt gtttaaccaa ggtggggact cgtggaattg ttgtctgaat
    11341 atggtttgtt tttggcgaaa atcgttaccg ttgtgctagc gattgcggcg attgccgcca
    11401 ttattgtcaa tgttgctcaa cgtaataaac gccagcgtgg cgagttacgg gtcaacaatc
    11461 tcagcgaaca gtataaggag atgaaagaag aactggccgc ggcgctgatg gactcacatc
    11521 agcaaaaaca gtggcacaaa gcgcagaaga aaaagcacaa gcaagaagcg aaagcagcaa
    11581 aagcgaaagc caaactgggc gaggtggcaa ctgacagtaa acctcgcgtc tgggtgctgg
    11641 attttaaagg cagcatggac gcccatgaag tgaactcgct acgtgaagag ataacggctg
    11701 tactcgcagc attcaaaccg caggatcagg ttgtgctacg tctggaaagc cctggtggca
    11761 tggtgcatgg ttacgggttg gcggcttcgc agctgcagcg tctgcgtgat aaaaacattc
    11821 ctttaactgt tacggtagac aaagtcgctg ccagcggcgg ttacatgatg gcctgtgtgg
    11881 cggacaaaat tgtttccgca ccgtttgcta ttgtgggttc cattggggtg gtggcgcaaa
    11941 tgcccaactt taaccgcttc ctgaaaagca aagatattga tatcgaactg cacactgccg
    12001 ggcagtataa gcgtactctg accttgctgg gtgaaaatac cgaagaaggg cgggagaaat
    12061 tccgcgaaga gttgaacgaa acgcatcagt tgtttaaaga ttttgtgaag cgtatgcgtc
    12121 cgtctctgga tattgaacag gtggcaacgg gtgaacactg gtacggacaa caggcggtag
    12181 agaaaggcct ggttgatgaa atcaatacca gtgatgaagt tattcttagc ctgatggaag
    12241 gccgtgaagt ggttaatgta cgctatatgc agcgtaaacg actcattgac cgattcaccg
    12301 gcagcgcggc agagagcgcc gatcgattgt tgctacgctg gtggcagcgg ggtcaaaagc
    12361 cattgatgta aaagacaaac gcgaggctaa gacctcgcgt tttgctttaa tcaaccagat
    12421 gatatttttc tgaaagcaca tgggccaggt gtttgaacat attaaacacc gcggtgcttt
    12481 tggctgttgg caatccttgt tcatctaaaa agtagtcgcc ggtaaatacc agcacgccat
    12541 tacgctgcgt gacgccggtg gcttcaatcc ctgcgagcgt atcctcatgc tcacgaatga
    12601 ttttgttggc ctctttcaac agcgtttcgc ggtcgatggg ttgtgtctct ttgttcatta
    12661 tttactcctt aaacaaggac attagtctac gccaggcatg gcttgcagac aaatatacca
    12721 cgctggtggc aagagcgcct tactggcaac tttggatttt gcatgctaat aaagttgcgt
    12781 atcggatttt atcaggtaca gtgtgacgct ttcgtcaatc tggcaataga tttgcttgac
    12841 attcgaccaa aattccgtcg tgctatagcg cctgtaggcc aagacctgtt aactcagtca
    12901 cctgaatttt cgtgaacaga gtcacgacaa ggggttgata tccgcagaga acgagtccat
    12961 atcggtaact cgttgccagt ggaaggttta tcaacgtgcg acgcattcct ggaagaatca
    13021 aattaggtaa ggtgaatatg ggtaaagctc ttgtcatcgt tgagtccccg gcaaaagcca
    13081 aaacgatcaa caagtatctg ggtagtgact acgtggtgaa atccagcgtc ggtcacatcc
    13141 gcgatttgcc gaccagtggc tcagctgcca aaaagagtgc cgactctacc tccaccaaga
    13201 cggctaaaaa gcctaaaaag gatgaacgtg gcgctctcgt caaccgtatg ggggttgacc
    13261 cgtggcacaa ttgggaggcg cactatgaag tgttgcctgg taaagagaag gtcgtctctg
    13321 aactgaaaca actggctgaa aaagccgacc acatctatct cgcaaccgac cttgaccgcg
    13381 aaggggaagc cattgcatgg cacctgcggg aagtgattgg gggtgatgat gcgcgctata
    13441 gccgagtggt gtttaacgaa attactaaaa acgcgatccg ccaggcattt aataaaccgg
    13501 gtgagctgaa tattgatcgt gttaatgccc agcaggcgcg tcgctttatg gaccgcgtgg
    13561 tggggtatat ggtttcgccg ctgctatgga aaaagatcgc tcgtggtctg tctgccggtc
    13621 gtgtgcagtc ggtggcggtt cgcctggtgg tcgagcgtga gcgtgaaatt aaagcgttcg
    13681 tgccggaaga gttctgggaa gtcgatgcca gcacgaccac gccatctggt gaagcgttgg
    13741 cgttacaggt gactcatcag aacgacaaac cgttccgtcc ggtcaacaaa gaacaaactc
    13801 aggctgcggt aagtctgctg gaaaaagcgc gctacagcgt gctggaacgt gaagacaaac
    13861 cgacaaccag taaacctggc gctcctttta ttacctctac gctgcaacaa gctgccagca
    13921 cccgtcttgg atttggcgtg aaaaaaacca tgatgatggc gcagcgtttg tatgaagcag
    13981 gctatatcac ttacatgcgt accgactcca ctaacctgag tcaggacgcg gtaaatatgg
    14041 ttcgcggtta tatcagcgat aattttggta agaaatatct gccggaaagt ccgaatcagt
    14101 acgccagcaa agaaaactca caggaagcgc acgaagcgat tcgtccttct gacgtcaatg
    14161 tgatggcgga atcgctgaag gatatggaag cagatgcgca gaaactgtac cagttaatct
    14221 ggcgtcagtt cgtagcctgc cagatgaccc cagcgaaata tgactccacg acgctgaccg
    14281 ttggtgcggg cgatttccgc ctgaaagcac gcggtcgtat tttgcgtttt gatggctgga
    14341 caaaagtgat gcctgcgttg cgtaaaggcg atgaagatcg catcttacca gcagttaata
    14401 aaggcgatgc tctgacgctc gttgaactta caccagccca gcactttacc aagccgccag
    14461 cccgtttcag tgaagcatcg ctggttaaag agctggaaaa acgcggtatc ggtcgtccgt
    14521 ctacctatgc gtcgatcatt tcgaccattc aagatcgtgg ctacgtgcga gtagaaaatc
    14581 gtcgtttcta tgcggaaaaa atgggcgaaa tcgtcaccga tcgccttgaa gaaaatttcc
    14641 gcgagttaat gaactacgac ttcaccgcac agatggaaaa cagcctcgac caggtggcaa
    14701 atcacgaagc agagtggaaa gctgtactgg atcacttctt ctcggatttc acccagcagt
    14761 tagataaagc tgaaaaagat ccggaagagg gtggtatgcg cccgaaccag atggttctga
    14821 ccagcattga ctgcccgact tgtggtcgca aaatggggat tcgcacagcg agcaccgggg
    14881 tattccttgg ctgttctggt tatgcgctgc cgccgaaaga gcgttgcaaa accaccatta
    14941 acctggtgcc ggaaaacgaa gtgctgaacg tactggaagg cgaagacgct gaaaccaacg
    15001 cgctgcgcgc aaaacgtcgt tgcccgaaat gcggcacggc gatggacagc tatctcatcg
    15061 atccgaaacg taagttgcat gtctgtggta ataacccaac ctgcgacggt tacgagatcg
    15121 aagagggcga attccgcatt aaaggttatg acggcccgat cgttgagtgt gaaaaatgtg
    15181 gctctgaaat gcacctgaaa atggggcgtt tcggtaaata catggcctgc accaacgaag
    15241 agtgtaaaaa cacacgtaag attttacgta acggcgaagt ggcaccaccg aaagaagatc
    15301 cggtgccatt acctgagctg ccgtgcgaaa aatcagatgc ttatttcgtg ctgcgtgacg
    15361 gtgctgccgg tgtgttcctg gctgccaaca ctttcccgaa atcgcgtgaa acgcgtgcgc
    15421 cactggtgga agagctttat cgcttccgcg accgtctgcc ggaaaaactg cgttatctgg
    15481 ccgatgcgcc acagcaggat ccggaaggta ataagaccat ggttcgcttt agccgtaaaa
    15541 ccaaacagca atatgtctct tcggaaaaag acggaaaggc gactggctgg tcagcatttt
    15601 atgttgatgg caaatgggtt gaaggaaaaa aataacct
//
LOCUS       DAPPNJ010000107        15310 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000107 DAPPNJ010000000
VERSION     DAPPNJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.108"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(164..1075)
                     /gene="gsiD"
                     /locus_tag="RPM73_002779"
     CDS             complement(164..1075)
                     /gene="gsiD"
                     /locus_tag="RPM73_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDX6521122.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1078..1998)
                     /gene="gsiC"
                     /locus_tag="RPM73_002780"
     CDS             complement(1078..1998)
                     /gene="gsiC"
                     /locus_tag="RPM73_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDX6521123.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2016..3554)
                     /gene="gsiB"
                     /locus_tag="RPM73_002781"
     CDS             complement(2016..3554)
                     /gene="gsiB"
                     /locus_tag="RPM73_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDX6521124.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRVSDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3574..5445)
                     /gene="gsiA"
                     /locus_tag="RPM73_002782"
     CDS             complement(3574..5445)
                     /gene="gsiA"
                     /locus_tag="RPM73_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDX6521125.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQASPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5432..6397)
                     /gene="iaaA"
                     /locus_tag="RPM73_002783"
     CDS             complement(5432..6397)
                     /gene="iaaA"
                     /locus_tag="RPM73_002783"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDX6521126.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6601..7836
                     /gene="moeA"
                     /locus_tag="RPM73_002784"
     CDS             6601..7836
                     /gene="moeA"
                     /locus_tag="RPM73_002784"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDX6521127.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7836..8585
                     /gene="moeB"
                     /locus_tag="RPM73_002785"
     CDS             7836..8585
                     /gene="moeB"
                     /locus_tag="RPM73_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDX6521128.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8661..9323)
                     /gene="fsa"
                     /locus_tag="RPM73_002786"
     CDS             complement(8661..9323)
                     /gene="fsa"
                     /locus_tag="RPM73_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDX6521129.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAHQIISYPAVDAAVAKFKQDWQGAFGRT
                     SI"
     gene            9454..10353
                     /locus_tag="RPM73_002787"
     CDS             9454..10353
                     /locus_tag="RPM73_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDX6521130.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMLPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10359..12791
                     /locus_tag="RPM73_002788"
     CDS             10359..12791
                     /locus_tag="RPM73_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDX6521131.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12937..13752
                     /gene="ybiV"
                     /locus_tag="RPM73_002789"
     CDS             12937..13752
                     /gene="ybiV"
                     /locus_tag="RPM73_002789"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDX6521132.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13903..15168
                     /locus_tag="RPM73_002790"
     CDS             13903..15168
                     /locus_tag="RPM73_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDX6521133.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHYDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3700 a         4237 c         3915 g         3458 t
ORIGIN      
        1 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
       61 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
      121 tgcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
      181 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
      241 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
      301 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
      361 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagacgaga
      421 cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
      481 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa
      541 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
      601 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
      661 gcatgatcag ccgatcccac cagccttcat aatatccagc cagcaagccc agcaacgtcc
      721 ctatcgccgc accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
      781 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
      841 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
      901 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
      961 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     1021 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
     1081 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     1141 aataaattcc agtgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     1201 tacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     1261 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
     1321 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
     1381 acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac
     1441 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
     1501 tcccacggta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa gcatccccag
     1561 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
     1621 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
     1681 catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac
     1741 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
     1801 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
     1861 ctgcgcatca gcttcaggcc cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac
     1921 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt
     1981 gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
     2041 ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
     2101 agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc ctgcgccgcc
     2161 ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc caggaagtca
     2221 tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag
     2281 gcaaacaacg gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag
     2341 aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
     2401 gcatccatcg cagtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
     2461 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
     2521 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
     2581 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag
     2641 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
     2701 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
     2761 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
     2821 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
     2881 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
     2941 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
     3001 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc cggtgaaatc
     3061 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagagta
     3121 atctttactg ttgttggatc gatcgcctcc gttttggcaa tattcttata caggttatag
     3181 cgtttaagat gattcgccgg atcgctgacc cggtccagat tcgcttttac cgccgctgcg
     3241 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt gtatgtaagg
     3301 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
     3361 agaccaaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcatttgcg
     3421 tcatacggat cgagcgtggt gaagttcgat cctaccgcca ccaccacatc tttggcagcg
     3481 aatgcacaag atgccatcaa cgctgtcgca atgcccaacg ccactaaccc actacggtgt
     3541 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
     3601 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
     3661 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
     3721 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
     3781 cgggttttcg aagaccgcgc gccgtggacc aatttcaaca atttgtccga gatacatcac
     3841 cgccacacga tgactaatcc gctctaccac ggccatatcg tgggagataa acagatacgc
     3901 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
     3961 atccagcgcc gagacggctt cgtcggcaat gatcactttc ggattcaatg ccaacgcgcg
     4021 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
     4081 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cttctttacc
     4141 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
     4201 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
     4261 tttgccgggt gacaaggtat cgattctctg accgttaaag ataatttcgc cgccctgcga
     4321 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
     4381 cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc
     4441 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
     4501 tcgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atgggggagg cctgtttcgc
     4561 tggatgttca agcgatatca acgggaaacg tcggggataa tctaaccctt tcatcgcacc
     4621 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
     4681 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
     4741 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
     4801 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
     4861 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
     4921 ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg
     4981 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
     5041 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaataccg ggttcagcga
     5101 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgaacatggc gcatttgtgc
     5161 agcgctctgc tcgctaagtt caatcacttc gcgactgcgc cgctgcaaca gcattttatc
     5221 gcactgtact aaaccgcccg cctgttccaa caggcgcatc aatgccaacg cagtcactga
     5281 cttaccggag ccggattcgc caacaattgc cagcgtctca ccgcgttgca gactaaaaga
     5341 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat tcagattttc
     5401 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
     5461 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
     5521 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
     5581 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
     5641 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
     5701 ccgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
     5761 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
     5821 ccgccaaatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
     5881 catccagtgg cgcaccgcta tggtcgagga cggttgcccc ttccttgcgc gctgccagta
     5941 gttgttcata acgcaaaggc gtggagaaaa tctccggcga gacgcgctcc atgccacgag
     6001 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
     6061 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
     6121 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
     6181 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
     6241 ccacatccag cgcactttcg cccgcttcca gcattttctg cccggtttca acaatggcag
     6301 acaacgcctc aatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
     6361 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
     6421 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
     6481 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
     6541 tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
     6601 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
     6661 gtcaccccac tgaccgccca ggaaacgctg ccactggtac agtgttttgg tcgtattctg
     6721 gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg ataactccgc aatggacggc
     6781 tacgcggtgc gtttagccga tattgcctcc aggcaaccgc tgcccgttgc cggtaaatcc
     6841 tttgccggtc agccgtacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
     6901 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
     6961 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
     7021 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgctgaa
     7081 ctgccagtga ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
     7141 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccactggg cgatggccaa
     7201 atctacgata ccaaccgtct cgccgtacac ctgatgttag aacagttggg atgcgaggta
     7261 atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc
     7321 gacagccagg cggatgtggt gatcagttcc ggcggtgttt cagtgggtga agcggattac
     7381 accaaaacga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
     7441 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
     7501 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
     7561 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
     7621 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
     7681 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
     7741 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa
     7801 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga
     7861 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
     7921 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
     7981 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
     8041 cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat
     8101 ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
     8161 tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta
     8221 cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaagtgccgc
     8281 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
     8341 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
     8401 aagcaggcgt gatggcaccg ttgatcggcg taattggttc gttgcaggca atggaagcga
     8461 tcaagctgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga
     8521 tgacctgtca gtttcgcgaa atgaaactga tgcgtaatcc ggggtgtgag gtgtgcgggc
     8581 agtaattgcc acctccaggc cagataagac gcgtcactcc gccacgtcat cagcaattta
     8641 tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg ccagtcctgc
     8701 ttaaacttcg ccacagcggc atcgacagcc ggatagctaa tgatctgatg tgcgacatcc
     8761 agcggcagag taattgattc acatcctgcc agtaagcagt ccagcgcctg acgcggggtt
     8821 ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag
     8881 tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa tacgattaac gtaaggcgca
     8941 acatattccg cacctgccag cgccgacagc agcccttgtg ctgcgccata taccgcggtt
     9001 cccagcgttg gaatcccttc cgcttttaac atcttaatag ctgccagccc ctcggcggtc
     9061 accggaactt tcaccacgat atccgcaata atagaacgca atttacgtgc gtcattcacc
     9121 atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc gcccatcgct
     9181 tcatgaagtt gcggaagcac aacatccagc ggttttttac ccgcggcgat aatgcttggg
     9241 ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct
     9301 gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc tgaatgtaga
     9361 ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc attcgaaagt
     9421 attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca gcgctactcg
     9481 acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc gctgggctgc
     9541 cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta tgacgcacga
     9601 ctgtgtctgg aaggctgcga gctgtgcgct aaggcagcgc cggaagtgat tgagcgcgcg
     9661 ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcattttac ggcgctaact
     9721 gactgctgcc cgacacaggc gttaaccgtg tgtggggaag tgaaaagcgt tgaggagatc
     9781 atgacgaccg ttctgcgcga taaaccgttt tacgatcgca gcggcggcgg tttaacgctt
     9841 tcgggtggtg agccctttat gctgccggaa atggcgatgg cgctactgca agccagccac
     9901 gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc
     9961 ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc cgacgcgccg
    10021 tttaaacagt ggaccgacgg taacgccgcc agagtgctgg ataacctgaa aaaactcgcc
    10081 gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa tgccgacgaa
    10141 acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg cgaaattcat
    10201 tttctgccct accacacgct gggcatcaac aaatatcact tacttaatct gccctatgac
    10261 gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca gtatgcctgc
    10321 cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg aaactggaca
    10381 cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg aaaccgccag
    10441 tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc gataagccga
    10501 tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc atctggatca
    10561 aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc
    10621 cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat cgtcccggtg
    10681 ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc ccgtggtggc
    10741 gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa aaaggtctgc
    10801 tggcgaccgg aatcattaaa gcggaaggca atatgacctc cggcgatgcg cacctggcgg
    10861 tgaatttccc gctgctgctg gaaaaagggc ttgatggtct gcgcgagaaa gtagcggaac
    10921 gtcgctcgcg catcaacctg acggtgctgg aagatttaca cggtgagcaa ttcctgaaag
    10981 cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct gccctggcgc
    11041 gtgaaatggc cgcgaccgaa acccgcgaaa gccgtcgcga tgaactgctg gcgatggcag
    11101 aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg ctgcaactgt
    11161 gttacttcat ccagttgatt ttgcagatcg aatctaacgg tcactcagta tcgtttggtc
    11221 gtatggacca gtatctctac ccgtactatc gccgcgacgt tgaactcaac cagacgctgg
    11281 atcgcgaaca cgccatcgaa ttgctgcata gctgctggct gaaactgctg gaagtgaaca
    11341 aaatccgctc aggctcacac tcaaaagcct ctgcgggaag tcctctgtat cagaacgtca
    11401 ccattggcgg acaaaatctg gttgatggtc aaccaatgga cgcggtgaat ccactctctt
    11461 acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt
    11521 accacgcggg aatgagcaac gatttcctcg acgcctgcgt acaggtgatc cgttgcggct
    11581 tcgggatgcc ggcgttcaac aacgacgaaa tcgtcatccc ggaatttatt aaactcggta
    11641 ttgaaccgca ggacgcttac gactatgcag cgattggttg tatcgaaacc gccgtcggtg
    11701 gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc gtgatgctgg
    11761 cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca caagaaaaag
    11821 cgttgtcggc aggtaacttc aacaacttcg atgaagtgat ggacgcgtgg gatacgcaaa
    11881 tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag
    11941 agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa
    12001 gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt ggcattgcca
    12061 acctcggcaa cagcctggcg gcagtgaaga aactggtgtt tgaacagggc gcgattggtc
    12121 agcaacagct ggctgccgca ctggcggatg acttcgacgg cctgactcat gagcaactgc
    12181 gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact gtcgatacgc
    12241 tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat aacccgcgct
    12301 acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcgtctatc tccgctaacg
    12361 taccgtttgg cgcgcagact atggcaacgc cggacggacg taaagcgcat accccgctgg
    12421 cagaaggcgc aagcccggct tccggtactg accatctcgg cccaacggca gtcattggat
    12481 cggtgggtaa actgcctaca gcagcgattc tcggcggcgt gttgctcaac cagaaactga
    12541 atccggcgac gctggagaac gaatctgaca agcagaaact gatgatcctg ctccgtacct
    12601 tcttcgaggt gcataaaggc tggcatattc agtacaacat cgtttcccgc gaaacgctgc
    12661 tggaggcgaa aaaacatccc gatcagtatc gcgatctggt agtgcgtgtc gcgggctatt
    12721 ccgccttctt caccgcgctc tctccagacg ctcaagacga tatcatcgcc cgtactgaac
    12781 atatgctgta atctcctcgg cccggcggcg tcgtcgccgg gctaaatcat tcacatcatc
    12841 aatttcatcc ttactttcat tcgaaatata atttgtgctc tacgtcacat tgttattaca
    12901 ctgttacggt ttcattttcg agccgggagc acacctatga gcgtaaaagt tatcgtcaca
    12961 gatatggacg gtacttttct taacgacgtc aaaacgtaca accaaccacg ttttatggcg
    13021 caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg taatcagtat
    13081 taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt cgcggaaaac
    13141 ggcgcactgg tttacgaaca tggcaagcag ttgttccacg gcgaactgac ccgacatgaa
    13201 tcgcggattg tgattggcga gttgctaaaa gataagcaac tcaattttgt cgcctgcggt
    13261 ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa
    13321 cactaccatc gcctgaaacc tgtaaaagat tatcaggaaa ttgacgacgt actgttcaag
    13381 ttttcgctca acctgccgga tgaacaaatc ccgttagtga tcgacaaact gcacatagcg
    13441 ctggatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct gattattccc
    13501 ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa
    13561 aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat
    13621 tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat
    13681 gataataatc atgaaggcgc gctgaatgtg attcaggctg tgctggataa cacatcccct
    13741 tttaacagct gacctctcta ccggagccat gcgctccggt tttcccttct ttttacatcc
    13801 tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttat attgtcacat
    13861 gcaaatcatc atagtttcga atgaaagtta tgcgaggtgg ttatggcttc tacttttacc
    13921 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    13981 gcgcagcttg gcgacgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    14041 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    14101 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    14161 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    14221 ctggactatc tggaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    14281 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    14341 atgcaggctc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    14401 acatttgaaa gtgatggaaa gcaatggttt aacccggatg taagcgtcat ctaccctgac
    14461 cgtatccgcc gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    14521 ggggcactgg aacgctggct gcttccagcg tatcagcgcg ttttcgccaa cgtctttaac
    14581 ggcaatctgg cgcaatatga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    14641 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gacagcgctc
    14701 tctgatatgc tgcctggtca ggggctgctg cacgtcgtgc ccattcctga agccatggcg
    14761 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    14821 cccggaagag tattgccggt atcagagcaa tggcatccac tgttgattga ggcgttaacc
    14881 agcattccaa aactcgaagc cggagactcc gtctggtggc actacgacgt catccattcc
    14941 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    15001 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    15061 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    15121 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    15181 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    15241 ctttcttaat gcctgatgcg ctacgcttat ctggcctaca aagtcctgca gattcaatat
    15301 attacaggta
//
LOCUS       DAPPNJ010000108        15284 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000108 DAPPNJ010000000
VERSION     DAPPNJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.109"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..2916
                     /gene="ehaG"
                     /locus_tag="RPM73_002791"
     CDS             <1..2916
                     /gene="ehaG"
                     /locus_tag="RPM73_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDX6521134.1"
                     /translation="AAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTT
                     NIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSS
                     DAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFS
                     TSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVV
                     DALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFS
                     AAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITA
                     PTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSIS
                     EDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFN
                     DASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTS
                     ITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVA
                     TTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGS
                     NAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAG
                     SADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKY
                     FKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSSTNQRRIT
                     NVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAG
                     VNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAY
                     TPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3285..4940
                     /gene="lldP"
                     /locus_tag="RPM73_002792"
     CDS             3285..4940
                     /gene="lldP"
                     /locus_tag="RPM73_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDX6521135.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4940..5716
                     /gene="lldR"
                     /locus_tag="RPM73_002793"
     CDS             4940..5716
                     /gene="lldR"
                     /locus_tag="RPM73_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDX6521136.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            5713..6903
                     /gene="lldD"
                     /locus_tag="RPM73_002794"
     CDS             5713..6903
                     /gene="lldD"
                     /locus_tag="RPM73_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDX6521137.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            7089..7562
                     /gene="trmL"
                     /locus_tag="RPM73_002795"
     CDS             7089..7562
                     /gene="trmL"
                     /locus_tag="RPM73_002795"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDX6521138.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7615..8436)
                     /gene="cysE"
                     /locus_tag="RPM73_002796"
     CDS             complement(7615..8436)
                     /gene="cysE"
                     /locus_tag="RPM73_002796"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDX6521139.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8516..9535)
                     /gene="gpsA"
                     /locus_tag="RPM73_002797"
     CDS             complement(8516..9535)
                     /gene="gpsA"
                     /locus_tag="RPM73_002797"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDX6521140.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9535..10002)
                     /gene="secB"
                     /locus_tag="RPM73_002798"
     CDS             complement(9535..10002)
                     /gene="secB"
                     /locus_tag="RPM73_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDX6521141.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10065..10316)
                     /gene="grxC"
                     /locus_tag="RPM73_002799"
     CDS             complement(10065..10316)
                     /gene="grxC"
                     /locus_tag="RPM73_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDX6521142.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10458..10889)
                     /locus_tag="RPM73_002800"
     CDS             complement(10458..10889)
                     /locus_tag="RPM73_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDX6521143.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11134..12678
                     /gene="gpmM"
                     /locus_tag="RPM73_002801"
                     /gene_synonym="pgmI"
     CDS             11134..12678
                     /gene="gpmM"
                     /locus_tag="RPM73_002801"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDX6521144.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12688..13971
                     /gene="envC"
                     /locus_tag="RPM73_002802"
     CDS             12688..13971
                     /gene="envC"
                     /locus_tag="RPM73_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDX6521145.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13975..14934
                     /locus_tag="RPM73_002803"
     CDS             13975..14934
                     /locus_tag="RPM73_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDX6521146.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(14921..>15284)
                     /gene="waaH"
                     /locus_tag="RPM73_002804"
     CDS             complement(14921..>15284)
                     /gene="waaH"
                     /locus_tag="RPM73_002804"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDX6521147.1"
                     /translation="LHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQIT
                     YEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQW
                     RDKTRSHHRITRSAFNLR"
BASE COUNT         3666 a         4055 c         4071 g         3492 t
ORIGIN      
        1 gcagctcatg gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg
       61 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
      121 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
      181 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
      241 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
      301 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
      361 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
      421 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
      481 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
      541 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
      601 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
      661 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
      721 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac
      781 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
      841 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
      901 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
      961 ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
     1021 aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg
     1081 tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
     1141 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
     1201 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
     1261 ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat taactatgtg
     1321 cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt tggcgctaca
     1381 gctataggtt ataactctgt cgccaaaggc gatagcagcg tagctattgg tcagggcagc
     1441 tacagcgacg ttgatacggg tatcgccctg ggtagcagct ctgtttccag ccgagtgatt
     1501 gccaaaggct cccgtgacac cagcataacg gaaaatggcg ttgttattgg ttacgacacc
     1561 acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata tcgtcaaatc
     1621 atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca attgcagaat
     1681 gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc aacggaagaa
     1741 gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc
     1801 gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact taacggcatt
     1861 gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg taatggttct
     1921 acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc accgcagaac
     1981 tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac taacgtcgca
     2041 gcaggttcgg ctgataccga tgcggtcaac gtgggtcagt tgaaagtaac ggatgcgcag
     2101 gtttcccaga atacccagag cattactaac ctggataatc gggtaacgaa tcttgattca
     2161 cgcgtcacca atatcgaaaa cggtattggc gatatcgtca ccaccggtag caccaagtac
     2221 ttcaagacca ataccgatgg tgtagatgcc agcgcgcagg gtaaagatag cgtcgcgatt
     2281 ggttctggct ccattgctgc cgcggataac agcgtcgcac tgggtacagg gtctgtggca
     2341 accgaagaaa atacgatctc tgtaggctca tctactaatc aacgtcgtat caccaacgta
     2401 gccgcaggta aaaacgacac cgatgctgtt aacgtggcac agttgaagtc ttccgaagct
     2461 ggcggtgtgc gttacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
     2521 ggtggcggca acggcggtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
     2581 gacgcggtga attacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat
     2641 cagcgaatgg ttgaaatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
     2701 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
     2761 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
     2821 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
     2881 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
     2941 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
     3001 tcaacaatct aagggcaatt ctctgatgag gattgccctt tttctttacc agacatctcc
     3061 ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
     3121 aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt
     3181 atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc
     3241 aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac
     3301 aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc
     3361 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcgtgga
     3421 cggtggcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
     3481 tggcctcggt ggtttatggt ttcttctacg ggttgtggcc catcgcgtgg atcattattg
     3541 cagcggtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt
     3601 ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg
     3661 gcgcgttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
     3721 tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg
     3781 cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca
     3841 tcgacagctt tgagattggc cagatggtgg ggcggcagct accgtttatg accattatcg
     3901 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaggag acgtggcctg
     3961 cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctct aacttcattg
     4021 ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc
     4081 tcaaacgctg gcagccagtg cgcgtattcc gttttggtga tttgggggcg tcacaggttg
     4141 atatgacgct ggcccacacc ggttacactg cgggtcaggt gttacgtgcc tggacaccgt
     4201 tcctgttcct gacagctacc gtaacactgt ggagtatccc gccgtttaaa gccctgttcg
     4261 catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac
     4321 tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt
     4381 ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct
     4441 ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
     4501 ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
     4561 gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct
     4621 cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc
     4681 tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg
     4741 ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta
     4801 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
     4861 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct
     4921 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     4981 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     5041 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctacgcgagg cgctggcaaa
     5101 actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     5161 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga
     5221 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     5281 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     5341 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     5401 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     5461 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     5521 actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc
     5581 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     5641 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcattatg agcattcgag
     5701 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
     5761 ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc
     5821 cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtct
     5881 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca
     5941 ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg
     6001 gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
     6061 gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt
     6121 atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg
     6181 gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat
     6241 gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
     6301 ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga
     6361 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
     6421 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg
     6481 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
     6541 ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg
     6601 gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg
     6661 cgtatgattg cgctcggtgc cgacaccgta cttctgggtc gtgctttcct gtatgcgctg
     6721 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgagaa agagatgaaa
     6781 gtggcaatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg
     6841 caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca
     6901 tagtcgtttg agccccctca ccctaaccct ctccctcagg gagtggggac cgttcggcgc
     6961 tgtatgtact ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg
     7021 gggtgagggg aaaaggccgc actgctcacc aattttctgc tatcctgccc ccgcactaag
     7081 ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaata
     7141 tcatccgtct ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg
     7201 cctgggacga taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta
     7261 cgcgtcatca tgactttcgc gcattcctcg aagcagaaaa tccccagcgc ctgtttgccc
     7321 tcaccacgaa aggtacgcct gctcacagcg ccgtaaacta tcaggatggc gattatctga
     7381 tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac
     7441 aaaaaattcg cattccgatg gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt
     7501 cagttgtggt gtatgaagcc tggaggcagc tgggatatcc gggagcgttg ttgagagatt
     7561 agttactgta tgccggatag atgattacat cgcatccggc acgatcacag gacatcagat
     7621 cccatcccca tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga
     7681 tggcttatcg ctgtctggtt taccgacaat acgagccgga acgccagcgg cggttgtatg
     7741 cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc
     7801 aatattgccg aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg
     7861 acggtcacca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc
     7921 gttttcaatt accgccgttt cacccacaac gatgcccgtg gcgtggtcga gcataatgcc
     7981 acggccaatt tttgctgccg gatgaatatc gacctggaac gtcacagaaa cctggttttg
     8041 cagaaagatt gccaatgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc
     8101 ctgcaaggca tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg
     8161 gtcgcgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc
     8221 ggcgtaggct tcttccacca cttcacggat agcaatagca ggcataattg gcgatgacag
     8281 cttgttcgcc agcatgtagc tcagtgcact accaaggttt tcgtgcttga gtagcgtcgc
     8341 gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat
     8401 attgttccag acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa
     8461 tgaccggccc gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt
     8521 ggctgctgcg ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt
     8581 tttttccgca atataatact tgataaattt cctcggttat tggcatttca acgccgaagc
     8641 gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct
     8701 tctcctgcgc gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac
     8761 gcgactggtt gtcggtacag gtaagcacca gatcgccaag ccccgccatg cccataaagg
     8821 tggcaggatc ggcacccagc gccgcaccaa gacgcgacat ttcagccagc ccacgggtga
     8881 tcagcgccgt acgcgcattc gcaccaaaac cgataccgtc ggacatcccc gcgccaatgg
     8941 caatgacgtt tttcaccgca ccgccaagct gcacaccgat gaaatccggg ttgctgtaaa
     9001 cgcggaaact tttgccacag tgcagcaatt gttggagatc atcggcaaag gtctggtcgg
     9061 tagaggccaa cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc
     9121 cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca
     9181 gacgtccggt ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca
     9241 tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg acgacaagaa
     9301 tattacggct ggctgccagt gcggtggcga gatcgctttc aagatggagc gtatcgggga
     9361 aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt
     9421 cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag
     9481 cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc
     9541 atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc
     9601 atcgaagtta accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt
     9661 gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc
     9721 catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag
     9781 gaacgcggtt tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtaaac
     9841 gtcatctgcc agttgagtag atgccgtatc cagatcaagt ttaacttctg gctgccaatc
     9901 tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagaa atatccttgg tataaatacg
     9961 ttggatctgg aaagtcattt cggtgttgtt ttgttctgac atgtgtagaa aacccttaag
    10021 tgttgtcctt aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga
    10081 tccagtccac cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg
    10141 tcaataaaaa tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg
    10201 gcggcgttgc catcgatcgg cagctcctgg aaactcacgc ccttgctgct cagcagtgct
    10261 tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataaat
    10321 tagctcctgt ttttttaccc tgaaatttca tgttgcaggg cggcagcaaa atggagtgtc
    10381 cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat
    10441 gaaagacgac aggtaaatta tttgccgcgc accaaaggca agttttcgcc agcccagcca
    10501 gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca
    10561 ggctcctggc actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc
    10621 tcaagctcac caacattgtt ggctttgatt tcgctcggca acagattaat agaacctgcg
    10681 atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg
    10741 ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta
    10801 aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga
    10861 cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa
    10921 aaaaaccagg aaaggagtat acctgcgcgg tgcggcaaat acagccagcg tgttaactgg
    10981 aatgcaattt tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga
    11041 gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg
    11101 caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta
    11161 ctggtgattc tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt
    11221 gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct
    11281 tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt
    11341 aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa
    11401 gatcgcgctt tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca
    11461 ggtaaagcag tacacattat gggtctgctc tccgtaggcg gcgtacacag ccacgaagat
    11521 cacatcatgg cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac
    11581 gcattccttg acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt
    11641 gaagagaaat ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac
    11701 gcgatggacc gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg
    11761 gcgcagggtg agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc
    11821 gacgaaaacg acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg
    11881 gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa
    11941 atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa agtggttaac
    12001 gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca
    12061 cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg
    12121 cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa
    12181 gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat
    12241 ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc aatcaaaagc
    12301 ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg
    12361 gtgatggaag cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg
    12421 aaagcggttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa
    12481 cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg
    12541 ctgatttacg ttggtgataa gaacgtgaaa gcggttgcag gcggcaaact ttctgacatc
    12601 gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg
    12661 ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg
    12721 gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct
    12781 ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt
    12841 caggccgata tcgccgcaaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc
    12901 ctgctcgcac aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc
    12961 gaaacgcaaa acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt
    13021 gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcgca actggatgcc
    13081 gcattccgtc agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag
    13141 cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt
    13201 gcccagttga agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa
    13261 caaagcgagc aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag
    13321 gcgctgaacg agcgtaaaaa gacgctggca gggctggagt cttccatcca gcaaggtcag
    13381 caacagttga gcgagctgcg cgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg
    13441 gaagccgcgg cgaaagcgcg tgcagaacga gaagcacgtg aggcccaggc ggttcgcgac
    13501 cgccagaaag aagcgacgcg taaaggcacc acctacaaac cgactgaaag cgaaaaatcg
    13561 ctgatgtccc gtaccggtgg tctgggcgcg ccgcgtggtc aggcattctg gccggttcgc
    13621 gggccgacgc tgcatcgcta tggcgaacag ctacagggtg aattacgctg gaaagggatg
    13681 gttatcggtg cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg
    13741 gctgactggc tgcaaggtta cggtctggtg gtggtggttg agcacggtaa aggcgacatg
    13801 agtctttacg gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc
    13861 cagccaattg cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa
    13921 attcgccgcc agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt
    13981 ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt
    14041 gctggcaaac ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag
    14101 gtgctggcga tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga
    14161 gagatggcga ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggca
    14221 ccactgagta aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa
    14281 attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac
    14341 cacatgggta gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcaggcactg
    14401 gagcgttaca atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc
    14461 gccgcgcaag gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag
    14521 aacgaagcgg acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt
    14581 tcaaccattg ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt
    14641 tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag
    14701 gttgatactt ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt
    14761 ggcgtgaagc tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt
    14821 gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg
    14881 caaggctggg gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc
    14941 tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc
    15001 acagtaacgc ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca
    15061 taccggaagt aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc
    15121 gcaccgcatg gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa
    15181 tcgtaatttt gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat
    15241 agtgacgttg ataattaagg tttttattcc cttgtctatg caac
//
LOCUS       DAPPNJ010000109        15107 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000109 DAPPNJ010000000
VERSION     DAPPNJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.110"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            104..1687
                     /locus_tag="RPM73_002805"
     CDS             104..1687
                     /locus_tag="RPM73_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDX6521148.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1961..3814)
                     /gene="asmA"
                     /locus_tag="RPM73_002806"
     CDS             complement(1961..3814)
                     /gene="asmA"
                     /locus_tag="RPM73_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDX6521149.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3836..4417)
                     /gene="dcd"
                     /locus_tag="RPM73_002807"
     CDS             complement(3836..4417)
                     /gene="dcd"
                     /locus_tag="RPM73_002807"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDX6521150.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4509..5150)
                     /gene="udk"
                     /locus_tag="RPM73_002808"
     CDS             complement(4509..5150)
                     /gene="udk"
                     /locus_tag="RPM73_002808"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDX6521151.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5468..8785
                     /locus_tag="RPM73_002809"
     CDS             5468..8785
                     /locus_tag="RPM73_002809"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX6521152.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGGLVVLLTAGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERTA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8824..9681)
                     /gene="alkA"
                     /locus_tag="RPM73_002810"
     CDS             complement(8824..9681)
                     /gene="alkA"
                     /locus_tag="RPM73_002810"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDX6521153.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9815..11167
                     /gene="yegD"
                     /locus_tag="RPM73_002811"
     CDS             9815..11167
                     /gene="yegD"
                     /locus_tag="RPM73_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX6521154.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11180..13120)
                     /gene="yegI"
                     /locus_tag="RPM73_002812"
     CDS             complement(11180..13120)
                     /gene="yegI"
                     /locus_tag="RPM73_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDX6521155.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISEAGNALETDIAHFRYAYASDNQLRGLKPPPRSIPLAMLPGHVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQHLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKFWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13117..13878)
                     /locus_tag="RPM73_002813"
     CDS             complement(13117..13878)
                     /locus_tag="RPM73_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDX6521156.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVCQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13875..14534)
                     /locus_tag="RPM73_002814"
     CDS             complement(13875..14534)
                     /locus_tag="RPM73_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDX6521157.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14755..14814)
                     /gene="ibsA"
                     /locus_tag="RPM73_002815"
     CDS             complement(14755..14814)
                     /gene="ibsA"
                     /locus_tag="RPM73_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDX6521158.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         3578 a         4009 c         3907 g         3613 t
ORIGIN      
        1 aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta tccatgatcg
       61 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
      121 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
      181 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc
      241 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
      301 ctggctggtc accctgacgc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
      361 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
      421 cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg cgaagttctg
      481 gggggtggtg acacaaattg tggtactgga cgccatcttc tcactcgact cagtgattac
      541 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
      601 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgaccatcgt
      661 tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
      721 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
      781 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacgctacg
      841 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag atgcggaact
      901 ggatgccgaa accgcgtcta tgctggtgga tcatggtaac cagcagatct ttaatccgca
      961 ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca gcagcattat
     1021 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagacg agatccgcca
     1081 gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg acgcagaaga
     1141 tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg gcgaaccgct
     1201 caacctgcgg gtgttgattc gccagccact ggtgttcccg gaaaccttgc cgttgttacc
     1261 tgccctggag cagttccgta atgcccgcac gcactttgct tttgtagtgg atgagtttgg
     1321 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg gtaacttacc
     1381 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aaaaatgcgg acggttcctg
     1441 gacggcgaat ggtcatatgc cgctggagga tctggtgcaa tatgtgccgc tgccgctgga
     1501 tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gagtatttgc aacgtattcc
     1561 aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc aggtggaaag
     1621 ccatcgcgtg cagaaggtgc agattattcc actgcgtaag gatggcgaga tggagtacga
     1681 ggtgtgatgg tatcggtcat tttacccgat agccgaaagg aataagatga gggaaccgcg
     1741 ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt gagccaccct
     1801 ggcacgttca ccggcttgag cgttccatag tagtgaggaa catcaagtga acggaacagc
     1861 cttcgatctt acatattcag aatataatgg tgccgccgcg aacgcctgat gcgacgcttt
     1921 acgcgtctta tcaggcctac aggacaggtc acctggtaac ctacatcttc tccagcaact
     1981 tcttcacatc tttgccattg cgggaatctt tattccgctc ggcccagtca ttcaggcgac
     2041 gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcaga ctgtaattga
     2101 gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttgagg aaatcaatca
     2161 gtttgctttc cccgttccag ccacccacga cccgaatatc aaactgggtg tcgcaggttt
     2221 gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat tgaccttgca
     2281 tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag cggtcaagac
     2341 gcgttacgtt atcgaagttt tcagcggcct tcacatcacc accattacgt tctaccgctt
     2401 gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt tcgacatgtg
     2461 cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg gagaaatcac
     2521 cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc agaatggtac
     2581 caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt gatgtcgcgt
     2641 ccagcgtgcc cggcagtgaa acctgtccac cgttaagctt gccctgcagt tgagtaattt
     2701 ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc
     2761 agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt ccctgatatg
     2821 ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa tgttgattgg ctctgcccct
     2881 gctgcgcggc accgttttca ccattcgctg tttcattaag cgggatgagg ttgtcaagat
     2941 tcagttgcgg aaattgcagt ctcagctgcc attcaggttt ctctgtcagc gtgacctgtg
     3001 cttgcccgct cagcgtgctg tcattggcgg tcaagctgat ttggttaaat gaaaggcgtt
     3061 tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct tgttttggta
     3121 aatctgcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa tcatgcggat
     3181 aatcagaagc atctaccgta ccgttaaggg atatcgtgag atcgcgctga tcgcgattaa
     3241 cccgcccgga gaactcaaat gaaccacgat gttggggatc ttgttccatt tgcaggcgaa
     3301 tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg ctgtccgcca
     3361 ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat
     3421 tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt
     3481 ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc agtagtggta
     3541 aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt gcaccctggg
     3601 cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc caacgcagtg
     3661 gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc atatagtcgc
     3721 ggaaatcatt cggattcacc agcaacacta acgcagataa cccggccacc agcacgacca
     3781 ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt
     3841 ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca tcttcacggc
     3901 ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca ccaattaaca
     3961 tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc acaatgcaac
     4021 cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca
     4081 aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc gactccagcg
     4141 tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg tcgagaacga
     4201 tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga ccgctcagat
     4261 cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg cgtacatcca
     4321 ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa cggccttcat
     4381 caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct ttcgcgcatc
     4441 actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg gtacacaatt
     4501 tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat cgcgatgcgg
     4561 tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc aatgaattgc
     4621 aggaacatcg ggcgcacggt tttttgatat tgcgccatca ctgaatccat tgaacgccca
     4681 cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg
     4741 aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa aatgccttcg
     4801 agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca
     4861 tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa atgctcaagc
     4921 agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc
     4981 gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat gtgttcatca
     5041 ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt gccggaagcc
     5101 gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat atatttagcg
     5161 acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg caattatagg
     5221 gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt taattgttga
     5281 caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa aaatgttata
     5341 cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca tataaaatgt
     5401 aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc ttgcggataa
     5461 agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc cgctgcttca
     5521 cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg agctttcgca
     5581 gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg tggcgtttta
     5641 tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg gaaatatcgc
     5701 cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga ccatcaatat
     5761 tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt ataacccctt
     5821 gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc cgcctctgtt
     5881 gggggggggt ctggttgtcc tgctgacggc cggagacgat cctctcaggg catttttgat
     5941 atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat tgttatttaa
     6001 accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt
     6061 agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc cttttacttt
     6121 cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag cctttttgat
     6181 cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct ccctgcttgc
     6241 tacgccgcgt acatacctga tgagccatat gccgtggcta ccgtttttgc tgatcctgct
     6301 gcccgccaac atcatgacga tggtgatgta tgcctttcgt gcggaacgca aacacatttc
     6361 cgaaagcgaa acccgttttc ggaacgccat ggaatattcc gctatcggta tggcgttagt
     6421 gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc tcgggtacag
     6481 tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg atctcaataa
     6541 agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt caatggaaaa
     6601 acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg
     6661 ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta acgagctaaa
     6721 acgcaccgaa caggtgaatc agcaactgat ggagcgcatc acgctggcta acgaagcggg
     6781 cgggattggc atctgggagt gggagttgaa gccgaatatt tttagctggg ataagcggat
     6841 gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt ggtacgagtg
     6901 cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt tgcaatcacg
     6961 ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc atatccgcgc
     7021 cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgcctgctcg gcattaatat
     7081 ggatatgact gaggttaaac agcttaacga ggcattgttt caggaaaaag agcgcctgca
     7141 cataacgctg gactccatcg gcgaagccgt ggtctgtatt gatatggcaa tgaaaattac
     7201 ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag cattaggtgt
     7261 tccgctcctg acggtgttgc atattacctt tggcgacaat ggtcccctga tggagaacat
     7321 ttacagtgcc gacacctcac gttccgcgat tgaacaagat gtggtgttgc actgccggag
     7381 cggcggcagc tacgacgtgc attacagtat tacgccgtta agtactctgg acggcagcaa
     7441 tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc tacgccagct
     7501 gagctacagc gcctcccatg atgcactaac gcatctcgcc aatcgcgcca gttttgagaa
     7561 gcaactgcgt atcctgctgc aaacggtaaa cagtacacat cagcgacatg ccctggtgtt
     7621 tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcacgccg ctggcgacgc
     7681 tttactgcgc gaactggcgt cgttgatgct gagcatgctg cgctccagcg acgtgctggc
     7741 gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgtcg aaagtgcgcg
     7801 ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatct gggaaggacg
     7861 tgtacatcgg gtaggtgcca gtgccgggat taccttgatt gatgacaaca atcatcaggc
     7921 ggctgaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg gtggccgtgg
     7981 ccgggtgacg atttacgaac cgcagcaagc tgccgcacat agcgaacgga cggcgatgtc
     8041 gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg cccacggtgt
     8101 cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta aactctggag
     8161 ttgcgaaggc gagattattg atgaacaaaa atttcgtcgt agcttcagcg atccggcact
     8221 tagccatgct cttgaccgac gggtattcca cgaatttttc cagctggccg caaaagcggt
     8281 tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga gtagcgccac
     8341 gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac ggttattaca
     8401 cctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa aactgcggct
     8461 ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct tcaactcgct
     8521 gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca acgtgcaggg
     8581 taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc gactcgggat
     8641 gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt ctggcatcgg
     8701 cgtcgatctg atttatggtg atgtgattgc tgatgcccaa ccgctggatt tgctggtgaa
     8761 tagcagttat ttcgcgatta actgagggag cgtttgccca ttgcctgatg cgacgtatca
     8821 ggcctacaat tcgtccgttc cgtctggttg ccagccttcc gtataccaga tatgcaacag
     8881 cgcataagaa cgccagggct tccagcgctc ggcataacgg cggatttgtg ccggtgtcat
     8941 ccccggaaat cgctgtttaa tcagataatc atccggcaga aaaacatctt tcgcctgcca
     9001 gccacgcaaa gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt
     9061 tttcatcgcc tgctccacat cgcccggaat tgtcattggt aagctgccct ccagcgccgc
     9121 atttgccaga tgaatcagtg cctctgcccg tttcaacggc atgcctaacg cttttaatgc
     9181 ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag cagatatact ccggaaaatc
     9241 atccagccgt tcgccataaa gctgtaccac tttggcagtc aattttgccg ccatcgccac
     9301 gctcaccagt tggcctaaaa tcgcccgcac accctgctca aaagcatcaa cacagccggg
     9361 taaacgcaat ccgggccgcg ccgcgcctaa cttgcccaac gcaccgttaa caatctgcgg
     9421 gttacattgc agatcaagca ggcggctcat tttcgccaga cactcagcgg caacaggttc
     9481 taaacctgca cttaaattta tgtgcagagt atgacgggcc atatccggaa tagcagtcac
     9541 tacgccgcga tattcgccca ccgccagact acgggcataa taatcgtcag cgaccgtttc
     9601 cacgccactc actgcacggg cggcgagaaa tcccaacatc cacgaccagt catacggcgg
     9661 ctgccagttc agggtataca tcgcatctcc ttattcatcc gctttcagca taaacgttat
     9721 tcagacgcta cgctttgctt tcatattccg gttgtcgcga cggcaacatt tcgctaaagt
     9781 cacgcccctt cttcaccggc atggggatta attcgtgttt attggttttg attacggtac
     9841 agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg caattactga aaatggaaaa
     9901 cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaagcag taagtgaatg
     9961 gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc tgttacgtcg
    10021 ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg
    10081 tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg tgaaatcacc
    10141 aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt ttgaggatct
    10201 ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc cagaagcgat
    10261 tactcaggcg gtgattggtc gcccgatcaa cttccagggg ctgggcggcg atgaagcaaa
    10321 cgcccaggcg caagggattc tggaacgcgc ggcgaagcgt gccggattca gggacgtggt
    10381 attccagtac gagccggtcg cggctgggct ggattacgaa gccaccttgc aggaagaaaa
    10441 acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg
    10501 gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca gtggttgccg
    10561 tattggcggt aacgatctgg acatcgcact ggcgtttaaa aacctgatgc cgttactggg
    10621 catgggtggc gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt ggaatgcggt
    10681 tgccatcaac gacgtacctg cgcagagtga tttctacagt agtgccaacg gtcgtctgct
    10741 taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgctac agaaagtctg
    10801 gcgtcagcgt ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa ttgctctttc
    10861 aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc gatgaactgg ctacgctgat
    10921 tagccagcaa gggctggaaa gcgccctcag ccagccactg gcgcggattc tggaacaggt
    10981 gcaactggcc ctggataacg cccaggaaaa accggacgtt atctacctga ccggcggtag
    11041 cgcccgttct ccgctgatta aaaaagcgct ggcagaacag ttgccgggca ttccgattgc
    11101 aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt
    11161 tcgttaattg aaaaatggct cagcagcggc ttaaatctgc ctgcgcctga gccagttttt
    11221 ctgctgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca tgaaggcgga
    11281 atcgctgcaa ctctgtcgcg ccgactgtca acgccgattc cagccgatgt cgtttggcgg
    11341 tcattttcgc cattaccgct tgtctgtctg ctggcgttat cgcttcattc ggcctgaaca
    11401 caaaacggcg ttcacagctc gctttccagt cgataaccgc ctgggtcaga tggtcgccaa
    11461 accctttcac ttgcttaacg ccacgacggg taacatctgc ggctgtttca ataccaaaag
    11521 accgtaacgc cgctttacgc gcagggccaa cgccgggaat agaggcaaca tcaataaaaa
    11581 atccctccag aaacttctgc ttctgccgtt cccttgcagt gtcatgaagt gctgccagag
    11641 cgcgtttctc ttcttccggt aacccgagaa tttcgtcctt cattttttcg agcatcgtcc
    11701 gtttggcgat aaaaccttcc aggccgccta cctgctggat ctggcggact aaatggtcat
    11761 agtccatttt cgcacgatta aatgcctcac ggcgttgctg aacttctgct ttgtacgctt
    11821 tgcttgtcag actgccaata atccagatag ccgccagcac aggaaccaaa ataatataac
    11881 gcggttctgc ctgaaggccg caaagcagca gcgataatgc tgacagtgcg atctcaatca
    11941 gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg ccggccagtc ggttggaaat
    12001 gatcgggtaa tggcaattgc aatgctggcg gtgctactga cgccatcacc atcgcccaga
    12061 atttcgccag cacaaaatca ccgctggtgg taatgacctc ttcgccgaga tcaataaaat
    12121 agataacgcc ttgattatcc agcgcacacc acgggcagtc agtcaaatga gcggggtaaa
    12181 catgcattgc tgaaacggta catttcttta aatgttggcg tagagagtcc agtgccgcta
    12241 cccacgcttt agccgtcgga cgcccggttg ccacgccact ttccgtgaat gcctgctgaa
    12301 acatggcttc aacatggccc ggtaacatcg ccagcggaat cgatcgtggc ggcggtttta
    12361 aaccacgtag ctggttgtct gacgcgtagg cataacggaa atgggcaata tccgtctcca
    12421 gtgcattacc cgcttcagag ataagcggta caccggaata aggatgccga ccaccaaaca
    12481 agacgtgaaa tatcagcaag gcaaggccaa aattatcgtg attcgcggtg cgttcaaagc
    12541 cgacaaatga cgacaaggtt tgtagctctg gcggcgtaaa atgcgacaca ccgacttcgc
    12601 ataaatgcaa cgtgccatta gcgttaatct gaaaggagtc gctatcaatc aataccactt
    12661 tgctgtcgcg acctaccata aaactgttct ggtttacgtc acccacgacg tgcccgtgct
    12721 cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg
    12781 cacaatgagg gtaacgttga cgacgatgtg ccgggctata gatcatatga atcggttctt
    12841 taccagaaac ttttggcatc ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg
    12901 ttgcctgcgg ccaggcgaca taattcaaca attgcgcgtc agctgtcgca gccataaagg
    12961 caagtttgtc ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg acgctatcga
    13021 caaactcatt gatatcataa accgcgcctt caccgccttt gcccagttca cggcctggcg
    13081 taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc
    13141 caacgtttta tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa
    13201 caattcaggt aataaatcga gttgttcctg cgttgctgac gccagtccat tgaaaaacgg
    13261 ggtaaagaaa ggtacatgag gagaattatc cagcatattt aacgccagtc gctggatacc
    13321 atccgtaaat gctgcaactt tatgcacacg ctcagtgctg gtgaaagttt ccagtctgga
    13381 aacggcatct tcatcggtaa tgaagtgcgt catattggca tattcaccca ccatcggcgt
    13441 aagcggcaat tgcagaccgt gaccaaaatc caccaccacg ccgccatcgc caatctgcat
    13501 aatcaaggtg ccgtcaggcg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac
    13561 tgctaattct ttggcttccg cctcggcaaa aagtcgctga caaacggtca gcacaatatc
    13621 cgtggcgagg atatcattaa gccccaattc cccaccctgc actttttgcg acatataagc
    13681 catcgcttca ttgacggcga gcgtcgcgcc ttctcctccc tgcgaaacgc tacctgcgcc
    13741 atcagcaagg aacatcacca gtaatggctg ctggtcattt agccaggcaa cctgcatctg
    13801 gcaggcatcc tggcaaggca agtccgcgct gatgtgcgat gttccaacag cggatgcata
    13861 aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac aacttccgtt
    13921 cccggcgtgg agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg
    13981 aattgtaatc cttgcagagg caaaggctga cgaacgctga tttgggccag cgtcttcata
    14041 tccgccccct gaacaccgat ggaaaagaaa gcaaaccttt tatcttcttc cccccgaaac
    14101 actttgttgg ctgcagcctg ccactcatcg gttggtgcac catcggtaat caggaaaatc
    14161 cacgggcgat aatacgaaat accatttgca cgatactcac gtttccgctc ctctaccata
    14221 tccagggctt tggtaatggc cgcacccatt ggtgtatcgc cctgggcaaa caggatgggc
    14281 ggaaaaaaat tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca
    14341 atgccgagtt ccactctttt taaagccagc ggatcggcaa gcagttcatc acgaaaggtc
    14401 accaatccgg cgttaagttc attaataggt ctgccgctca tcgagccaga aacatccagc
    14461 agcaaaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg
    14521 atttgttcgc tcataagaaa tcctttctta gagaattatc gaggtccggt atcataccga
    14581 ctgcaataat gaaataagag caaacgaaga aactttcaga attaggcccg gcggcgcaga
    14641 aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg
    14701 acataatttc aggagtgagg gttagggaga ggtttccccc tccccctggt gttcttagta
    14761 agcctggaag ctaatcacta agagtatcac cagtatgatg acgtgcttca tcataaccct
    14821 ttccttatta aaagccctct tctccgggag aggctttccc gtttcagcgt cccgctgaaa
    14881 tcatcggctt acctcctttc gccctgaatg cagtctatcg ctaacgcgtg aataaaatag
    14941 tttcctctgt gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac
    15001 tacaaagcct gcggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga
    15061 gcggggtttc ccccgccctg gtagtcttag taagcgggga agcttat
//
LOCUS       DAPPNJ010000110        15032 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000110 DAPPNJ010000000
VERSION     DAPPNJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.111"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..390)
                     /locus_tag="RPM73_002816"
     CDS             complement(<1..390)
                     /locus_tag="RPM73_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDX6521159.1"
                     /translation="MWLLGAGTNAGMQYADSGEINPVNSVAAGWINVITMGQGWKGTI
                     AWNAAGGALTNAINGDDPLTGAITNGAGAGFGYGVGNYVVKPVANTAGKWITGGWNPK
                     FDPTLLKYTEVKGQLGISKEMVPSKIPG"
     gene            complement(622..1077)
                     /locus_tag="RPM73_002817"
     CDS             complement(622..1077)
                     /locus_tag="RPM73_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521160.1"
                     /translation="MMYLLLGIALSAFTVGTVMFYRFSKVTGRSVQSPFTPMFFFDAL
                     FFGLGSRRDMNIAACAFMVFLLSVWGLTYIKLRTNFWGSPESYFGLGIVGIFFLIMHC
                     RYGAKDVVIKNDGLAPIRELINVHQSGLTSPFLWLSRAGYLGCLIGMIG"
     gene            complement(1082..1591)
                     /locus_tag="RPM73_002818"
     CDS             complement(1082..1591)
                     /locus_tag="RPM73_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521161.1"
                     /translation="MPPRVQQDASLAFDLSGKGVTNDEIIKAVDKSHIGPSVGDTAKI
                     HGDVKGQVAGGYVAGGYLEGVLSEDKFSINGGVTKVLGWRADVSAGLTFGPYPIKDFN
                     PTYDYSGAISTGIFSVEGSIGKDGFGGSFRVGGGIGASIRQSENSNNQPEINGSGSTE
                     LISWPIKKD"
     gene            complement(1614..1871)
                     /locus_tag="RPM73_002819"
     CDS             complement(1614..1871)
                     /locus_tag="RPM73_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521162.1"
                     /translation="MKYSSRVVIGKFIICSSILLLCFIGMILAAIITKLTFVALAWFQ
                     SGIFEVLWSDIVYAAQLGGLGGGILGFCWVLLYLFRAKGFS"
     gene            complement(1868..2473)
                     /locus_tag="RPM73_002820"
     CDS             complement(1868..2473)
                     /locus_tag="RPM73_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence"
                     /protein_id="HDX6521163.1"
                     /translation="MATCAENPSGAACQKAMNERDAVGLALATGTVALLPGSAQAMWG
                     LGASANAGISYLADGTVDPANAAIAGWVNVFSMGNGLAGTVGWNAAGGALGNWIDNKD
                     PLSGAISNGIGSAAGYGIGKGVTWGTDKITNAVGKWVTNGWDPKYDPTWIKYAEVNRN
                     DIIREGMKPSNVPGTAGDIGGSIGSELGGKVTDKVSEELKK"
     gene            complement(3651..12770)
                     /gene="cdiA"
                     /locus_tag="RPM73_002821"
                     /pseudo
     CDS             complement(3651..12770)
                     /gene="cdiA"
                     /locus_tag="RPM73_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
     gene            complement(12783..14555)
                     /locus_tag="RPM73_002822"
     CDS             complement(12783..14555)
                     /locus_tag="RPM73_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDX6521164.1"
                     /translation="MQRRQNNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGILHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVTALDGGWLHSDRDDPYSSGTLWGAAAGLSTTSGHVSGSFTAG
                     LPLVYPDWLAPDHLTVYWRVAVAF"
BASE COUNT         3304 a         4399 c         3593 g         3736 t
ORIGIN      
        1 ccccggaatc ttgctcggca ccatttcttt ggagatacct aactgccctt taacttcagt
       61 atattttagt agagtcggat cgaatttagg attccagcca ccggttatcc acttgcctgc
      121 tgtatttgca acaggcttca ccacataatt gccaacccca tatccaaacc cggcccccgc
      181 tccatttgta atcgccccag taagaggatc atctccgttt atagcgttgg ttaatgctcc
      241 acctgccgca ttccaggcta ttgtcccttt ccagccctgc cccatggtta tcacatttat
      301 ccagcctgct gccactgagt tgaccgggtt tatttcacca ctatctgcat actgcatccc
      361 ggcatttgtc ccggcaccaa gtaaccacat agcctgggca ctccccggta acagcgaaac
      421 actacttgtc gctaatgcgg ctgcataagc tttattttca gcctgccccc gcttacaggc
      481 ttcaccggaa ggattatcac tacaggagag aacatcagcg ccatgctctc tggctttttc
      541 cgtctggagc cattcactgc cacccagcag gttattctca acaactaatt ggcatttatc
      601 acaaatttca ttaacactgc atcagcctat catgccaatt aaacatccga ggtaccctgc
      661 cctacttaac cacaaaaatg ggctggtgag gccagactga tgaacattaa ttaactccct
      721 tattggtgca agaccatcat ttttgataac aacatccttt gcaccatagc gacaatgcat
      781 tatcagaaag aaaattccga caatccctaa accaaaataa ctttctgggc ttccccaaaa
      841 attagtccgt aattttatat aggtgagccc ccaaacactc agtaaaaaga ccataaaagc
      901 acaggcagca atattcatat cgcgtctgct gcctagccca aaaaatagag catcaaaaaa
      961 aaacattggg gtaaaaggtg attgcacgct tcttcctgtc actttactaa atcgataaaa
     1021 catgaccgtt ccgacagtaa aagcagaaag tgcaatacct aacagcaagt acatcatttg
     1081 gttagtcctt ttttataggc caagaaatta gttcggtact accagaaccg ttaatttccg
     1141 gctgattatt actgttttca ctttgtcgaa tactcgcacc aattcctcca ccaactctga
     1201 aagagccgcc aaagccgtct ttaccaatac tcccttcaac tgaaaaaata cctgtcgata
     1261 tagcaccaga ataatcataa gtaggattaa aatctttaat cgggtaaggc ccaaaagtca
     1321 acccggcaga cacatctgca cgccagccta ataccttcgt gaccccgcca ttaattgaaa
     1381 atttatcctc tgacagtaca ccttccagat accctcctgc gacataacct ccagcgacct
     1441 ggccttttac atctccatgt atttttgccg tatcacctac tgacgggccg atatgtgatt
     1501 tgtctaccgc tttgataatt tcatcgttag tgactccttt accactgaga tcaaaagcca
     1561 gggaagcatc ctgctgtacg cgaggcggca caagctcaga cagcgcatta ttctcaacta
     1621 aagccctttg ccctaaaaag atataacaaa acccaacaaa aacctaatat gccccccccc
     1681 agaccaccca actgagcagc ataaacaata tcgctccaca aaacttcaaa aatcccgctt
     1741 tgaaaccatg ctaacgctac aaacgttagt ttagttatta tggccgctaa gatcatgcca
     1801 ataaaacaca ataagagaat tgaactgcat ataataaact tacctattac cactcgagaa
     1861 gaatatttca tttcttgagt tcctcagata ctttatctgt tacttttcca cctagctcag
     1921 atcctataga accgccaata tctccagcag ttccaggaac attgctcggt ttcatgcctt
     1981 ctcgaatgat atcgttacga tttacttcag catattttat ccatgttgga tcgtacttag
     2041 gatcccagcc atttgtcacc cattttccta cagcattcgt aatcttatct gtcccccaag
     2101 taacaccttt accgatacca tacccggcag cagaaccgat accatttgaa attgcaccag
     2161 acagaggatc tttgttgtcg atccagttac ccagcgcacc acctgccgca ttccagccca
     2221 cagtccccgc caggccatta cccatgctga acacattcac ccagcctgca atggctgcgt
     2281 ttgccggatc tacagtacca tcagccagat aactgatacc agcattcgca cttgctccca
     2341 gtccccacat agcctgagca ctaccgggga gtagtgccac tgtacctgtt gccagagcca
     2401 gccctacagc atccctctca ttcattgctt tctggcaggc ggcaccggaa gggttttctg
     2461 cacatgtcgc catgtcgata ccatgctggc gtatccacgc agcctgagca tcttcactac
     2521 cgccaagtaa attattctca acaacaacta tttacgatag tatcttcgca tttgtagata
     2581 gagttgtaac caaatcccag cacctcccca taagccaact actgcgccaa ttattccgta
     2641 ttttacatac acctcagaag gtattggttc ccgagctata agccagatac taaccactat
     2701 tccaatagag caagtagcta tagatataag taacaactta ataagaagta gtagatgttt
     2761 tttcattttt tgtctccatc aacttctttt tgcgttgcgc tgcttgcacc ttctgatacg
     2821 aatgaaccaa caaatccacc aaatgttcca ggtatagagc tcatttggcc gccctgatac
     2881 ataccgtatc cgatagactt ttccgaatat tgcagtgaat atttactggc gtccattccc
     2941 agttgatttt tgatgtattg agcttctaac ttattagtaa ttgaagcacc aatgttatag
     3001 ccaaggcctg taccagccat gctccccatc actgacgacg tgctatcctg gccttcaatc
     3061 tggctgctca tgtaagcgct accggcaccc aacaactggt tagcgtaata tcctttcccg
     3121 gctgtaccta caccagtcaa acctgacatc atgaaactga gataatcgaa cttatcaccg
     3181 atatttcccg taccaatttg taccgcacca ttagccccat agctcagccc ggcactaata
     3241 aatttttcag tcagcgtggc tgatgatgtt aacaatgaac ccgctccggc aaccgtaccc
     3301 cagtaatttt ccggttcccc aaagaaagcc agagccctgt cacccttcga cgtattactg
     3361 ttcagatagt tcaggtcgta gctgttgcct tcagccacgt aatccttcac ttcctgatta
     3421 gtcagtgaac catataacca gccaggtcga cctgccgcac tgagtccgcc ctcaatattc
     3481 catttatctt caccagacaa ggccgtcaga ggatctgcgg ccacatcaat gtgtaatttc
     3541 tgtcggttct ctgcgcttaa tacagcatat ttattctgaa tatcgccgca gtctccaccc
     3601 gctttacgac aggccgtcat ttctctgtca aaagcatgcg cctcgtcata gctgaggtag
     3661 ttattctcaa cagcattctt cccgctctgt gctcccactg ctactgatgc agtgctgttt
     3721 ccggtaagac cgccggccag ccctgctgaa acggttgcca gcgtacttat cgtctgcttc
     3781 tgctcttcac tgaggtcaga ttgcttcacg ccaggataca gcattccggc aattgctctg
     3841 gctgccaatt ctcctgttgc ggcgcctgct gcaccggcag caacattatg accctgcatt
     3901 gccgctgctg caccgcccag aatggcgtga gcaatggcgt ttactgccgg gtatttttct
     3961 gttgatttca gcagatgcac cagttccggt gcagaagctc cggccagagc acccgctaaa
     4021 ttaccaccca ccagtccctg aagcgcggcc gtcgcggcct ggataccccg ctgaatttca
     4081 ctaccagtgc catacttcgc catttcattc ctgtaagcct gcgtgtcacg cagtttcgca
     4141 agatattcct gacgttgttt ctctgttgca ttagcaggaa gtgtttcgcc ggttgcttcc
     4201 ttcgcggcct tcaacgcatt cagttctccc tgtgtacgcg caatatccgc cacctgactg
     4261 cctatgtcac tgataagccc cactgtctgc agacgcctct gctctttctc cttgtcaaat
     4321 atcgggctga tactgtcatt agcgtgcgca gggtcacggc tcaggttcgc cagattctgc
     4381 ttctgattgt ccctgtcccg gatggtgatt gtgccatctg ccactgcggc ctgagtcgtt
     4441 ccttccgcat gtccgctgtg acctccggcg gatatcatgc cacccggcat gttaccctga
     4501 aatttatccc cgaagctgcc accaccgctc agactgattc cactgtgact gactttataa
     4561 tccgcttcgt tgtgaaggtc actgaacccc agcgttccgg tatccaggtg gtttttatcc
     4621 ggtgtggcag tggaggcaat caccgcacca tccagttggg tatgtctgcc cactgtaatg
     4681 tcgaaaccac catcaccggc aaacattccg gtttgttcag caacggagtc aaagcggctc
     4741 ttcatcttat cccgggaggc agcgatgtaa cctgagccgg tcatggagcc aaaggtaaaa
     4801 ctgccgccgg cagccacgct ggtctgttta ctgtcgtact tactggtgtc ctgctggctg
     4861 cttatcagca ggtcgtggcc cacatcggcg ataatcctgt tgccgttgac ctgagcaccg
     4921 ttcagtaccg tatcccgacc actgttgatg gtgacggttt taccgctatc tattgtggtt
     4981 tcagtccact cagtaccgtt acctttctcg ctgccttttg ccgcattaac gctggcaaag
     5041 acgctgatac cggcaccgtt accaccggca ccgatactga cacccacgcc accgccactg
     5101 ctgctgttcc tgcccgttgt tttttgtgtg tttgcagcgc cactcaacag aacatcattc
     5161 gcggcatcca ggcttgtgtt accaccggcc ttaagctggc ttcctgcaat cacaatatct
     5221 ccgcggttat cgcccctgtt tttgccggtt gcgacaacag acagattatt cccggcattc
     5281 agcgtactgc cggatactgt gtcactttca gaatgttgtt gtgatttcga tttctgggtg
     5341 gtaagcgaca ggctgactcc cgtcgcattc gggtcaccgg ttgcggtggc cattgccgca
     5401 gcctgtccgg cctgcacacc agacagcgct gtctttgtgg cctgcagggc tttcagacgg
     5461 ctgtcactgc tctccttcgt ctcctgtgca ctggtgaccg cattattgat ggcactgccc
     5521 accgtgccgg aaagggcaac cgtcagcccg cttttcttct gctcaaattt ttcgtccaca
     5581 gtacgacggt catgccccgg gtcaaccact acactgtcac cggtaatgct gatatcccgg
     5641 ttcgcaatca catccgaacc gctgatatga gcctgtttgc ccgcggtaat actgacatta
     5701 ccggcagtgg agccgatggt gctggcactc tgactctgcg ttgtcccggc ctcgcggcgg
     5761 tcgtgcgttg tcttactgct gccaatggtg aaaccaatac cgccagtacc catcagaccg
     5821 gatttcttcg tttccttaaa gcgccaggac gtatctgtac tggtggcagc aagaacatca
     5881 acatggttac ccgccgccag tgacacatcc tggtcagcca ccacatccga accctctacc
     5941 gtcaggttat caccggcgtt aacggtcacg cggttccccg acagcaggga gcctgcttca
     6001 cgggaggcac tgtcctcact gatggtgtgg gtggttttct tactgagaaa acctccgctt
     6061 tttttcttcg tttccagata gtgatagtca ctttctgtcg ccgtggtcag ggcaacatca
     6121 cgaccggcat tcacgctgat attgccggtt gcggtaacgg atgacgcaac agcggtgata
     6181 tcccgtcctg cggtgacggt ggtgtcacca ccgctggcga tttccgttcc ctgctgacgg
     6241 actgtctcgt taatctcttc ctttttcttc gacgtatagc tgtcacctgc gccggcagac
     6301 tctgccacca ggttcacatc acgcccggcc cggatggaca cgttattttc cgcagccaca
     6361 ccggcagcct gactggcaat atcacgaccg gcaacaagga ggaggttatc gcccgccgtc
     6421 accgtggaca ctgctgcgtg gctatcatga ctttctgacc tgccgttgcg actgtttttg
     6481 ctttccctga ctgcattcag actcaggtcg ttacctgcag aaagcagggc tctgtgcccg
     6541 gcagaaacag aggatgccgt gacatccaga ttatggcctg cagccatcgc caggttaccg
     6601 ccggcgctga tgctgctgcc ctgtgaggtg gtggatgatg cactgttgtc atcagtgtgc
     6661 cagaaaccgg actgactttt gctcccgctt atcaggtttg cggcaatgtt gatatcatta
     6721 cccgcagaca ttccaagggt tccaccggac gagaccgttg ccccggtaat atcaatgttt
     6781 ttccctgcat ccagtgaaag tgaatcagtg cctttaatgg tcgcaaccgg accggtgtcc
     6841 gtaccgctga gatgcacacc accatatcgg ctgtcactgc ccgcattcca ttgctgacgc
     6901 cgggtgatat tgctgatgtt gccactcacg ctttccagtt gtacggtttt accgctgatg
     6961 actgagctga tattgctgat atccccgatg gcgctcaggt ccaggctacc gcccgcgctt
     7021 atcagccccg cattcaggtt gtcgatatag ccggtactgt cgagcgagag gccgttttgt
     7081 gcgttgatgc tgccgccgct gttggtgata ttgccacccg caagctgcac gttgttcccg
     7141 ctgataacgc tgccgttatg cagggtgatc tcttccggcg acagatacag tttcgggacc
     7201 atgactgtct gtccgttgat ggtggctgac tcccaccaca gtatgctgcc gtcaagctga
     7261 gcaatctgtt cagctgtcag cgccacacca aactctaatc ccagtccttt ctgttgtctg
     7321 gccgcgttat ccatcagata ccgcatctgt tccgtgtctg aacccagtcc gttgatataa
     7381 cgtgaacccg tccggctcag caccgcgtta ctgacatacc gggtatcaaa gaccgcatcc
     7441 cccaggaaac gataatcttt ttccggtttc agcccgaggc ggtcaagaaa atacgatgag
     7501 cccagaaact gtttttcatc ggtatacgac ggagccgttt cacgtggcgc ctgacccggc
     7561 ttcgctccag gaagctcata cagtccggca aacaaatggc tgtccacctg tccgagacca
     7621 tccagtttcg ggtttaccgt aatcagatac ggactgtccg ggtccgtgga cggaaccagg
     7681 tatccattgt tgccggaagg cagtggccag tcatcactga taccggtctg accggtcagt
     7741 ggcgaacctc cggcaatatt tttcagggca cctgccagtt catcgtgcca ttgcggagag
     7801 ccaaccacca ccggctcata ctgctgcagc gctgtctgtg tcagactgtc tccgccggtc
     7861 tgctgactta acgtattcag tacaggtgca gagaccaccg gactgacact acctgcatgt
     7921 gcagtggttg ttccgttatt gatactgctg gtaaaacggg tcttaacatc cccgcccgcc
     7981 tgaataacgg aataatacgt cttaccgggc gtgtaatctt tttcccggcc atccagtgaa
     8041 aatctgatgg tattgttttc aaattccggt gacagcaggg gcagtttatc cagagagcct
     8101 gtggcatagc taccgtaaaa cgttttcggg tcgtagcggt ataccagata ttcattctct
     8161 gtccccgtct gccagctctg attgcttaac tctctgcccg aaagtgcgat atccccattc
     8221 gcaaggataa atgacgcccg gttttccagt cgttcagcct cagcagaaag attacgccct
     8281 gacgcaatgc ggcctgccgg attatcagca ccggttactg ttgtgatgtt ctggctgctg
     8341 agaaagcgct gtgtggcact gtcagcaaac ggcgcgtaat aataaagcgt atccattgtg
     8401 atattgcatg ccccgtgccc gttgcagggc gtaccgtgct gattttcaac ttcacgggtg
     8461 aaatagccat agctgccgtc aggaagaagg gaaaggggaa tatcaaccag agcatttccc
     8521 attccctgaa tggatgaggg gttagtccgg gttgttgttg tggcagaaaa tccctcccgc
     8581 tggttcagaa gatgcccggt tcttacaaca atatcgccct gatgcgtctc aatattcccg
     8641 gaggtattga taatctctgt gtttgcaccg ccggaagcat ccttctgtat ccacagactg
     8701 ttgccggcca ggatatcacc atgctggttg tgcagacggt ctgtaaacag cttcaggtta
     8761 ttccccgcat aaatcagcgc actgttcagc agggtaccgg ccacattcat tgtcagactg
     8821 cctgccgtgc cggtaaaacc actgatggtg atatcactcc ggctgttcag actcacatcg
     8881 ccaccggcct gaagtgaacc cggtgcgtta aggaaaagac gctgtgcgct gaaaacactg
     8941 ttgcctttac cggcagtcag cattccattg ttggtgaatg cctctccggc accgagcacc
     9001 atggcatcac cctgcatcac accgccgttg gtgatggcat tttgcgacgt gacggaaagg
     9061 gttttccctg cggccagggt accatgattc gtgagggcag caatcagttt cagtgtgaca
     9121 tcaccggtgg ccaccacctg accctgacca ctgaagtcct gagcgtcaag cagcaggttg
     9181 cctgcactgt acagccgccc tgtaccattt tgcagcagtg aactgccctt gacgccaagc
     9241 ccggaggttc ccagcagggt accgctgttg ctgaatgtgt ggtaattcac cagcaggtcc
     9301 gcaccctgaa gcgtaccggt attattcagc gtggttcctt taacgtcggc actgccggtg
     9361 gcaagtacgc gtccgccgtt gacagtattc accacatcca gcagcagggt ggcagcctgt
     9421 accagtccgc tgccggtgtt cgccagcacc tgcgccgtca gcgtgaggtt actgccggag
     9481 aggattttgc cgtcgttctg cagacggtca gtggcgttca gggaaacccc gccaccgccc
     9541 tgtatcgtgc cctggttact cagggtcgca gtactgacat tcagtgcatt ccggctcatc
     9601 agaacaacac cggaacggtt gttcacgcca ccggaggcgg ccagcgtcag cgtttcgccc
     9661 tgcagatgcc cgccgtttgt gagttgtcct gccgtgatgg tggtggcatt tccctgtaat
     9721 tgcccgtcgt ttgtgacact gtctgccttc agcgtcagca cacctgcact gagcagtttt
     9781 ccgctcgcgt gattgttcag cgtctgattc accgtgagcg tgagagcatc cacaccggtg
     9841 atgtcacccg cactggtcag tgagttcgcc ttcagagtca gattttttgc aatccattgt
     9901 ccgctgttgc ttaaattcag tgcactgagc gccatttcac cgttcgaggt gactttgctg
     9961 cccgcggcgc tgacaagctc acccgtcaga cgtgcagtca ggctgtcagc cgcctggatc
    10021 gccccgctgt ttgccagact gtctgcggtg atcagcaccc gtttgccctg ccagtgtccg
    10081 gaactggtaa tactgcctgc ggtgattgtc agatcgccgc tggtcagcag tgaacctccg
    10141 ttattcatca gcgcaggttg aggggatacc atacgggcgg caagcgtcag cgcggcgatc
    10201 ccggtgaacg tgccactgtt gatgacactg ttctggcgaa tcgtgacatg gtcaccctgg
    10261 acagtgccgc ggttatccag tgaatttcca tcaagggaga gcgtgccggc cgaaagcaga
    10321 ctgccccggt tgtccgtggt ggctgcgttc agcgtggtgt caccctggct catgatatcg
    10381 ccggtactgg tcaactgacc ggttgccgaa gcagtaaggt taccggttgc cagcacggag
    10441 ccactgttcg cccagttgtc cgcctgcacg gtgagattct gtccctgcgt ggtccctgcg
    10501 gtatgcagtg ttttaccccg gagggagagg tcgcccgccg tcagccagcg tccgttacgg
    10561 ccctgtgaga gggtgtcgcc tgcaagcgcc agtgcaccgg cgccctgcaa cagaccatca
    10621 ccatccagcg tggtcgccct gacgttcagc gtgtcagcga tgatttttcc cggattgctg
    10681 agggaggcag tgtttaacgt taaaccatta tcaccggtga taagcccgct gttgcggatg
    10741 tccggtatat ccagcgtcag gtctgcagca ctgtacagcg tgccgttctg ctgattatca
    10801 agcctctgtg tgttaacggt gagtgaggcc tccccctgca acagaccact gttggtcagg
    10861 gtctgtgact gtgtattcag ggcggaacca acaagcacac cgctgctggt cagttccggc
    10921 gcactgaggc tgagcgacgg ggcgctgctt ttcccgctgt gggtgagctt tttactggcg
    10981 ttcaccacca tggtctgttg tgctgcctgc gtacctgcaa gacgtgcatc tctggcgttg
    11041 atgctgagat ttttgccgct ctgaagttgt gcgcccgctg cggtactcag tttgtctgcc
    11101 tgaaccagga gggtgtcacc ggcactgttt tccccgtcca gcgccactgt tgtcacattc
    11161 agcgtcatcg cagcatcgct gtgggtgacc gattttttac cggagctcag cgtctgcgca
    11221 ctgaccgtca gccctttgcc gccggacagc acaccgttct gtgtcacatc ctgcgccttc
    11281 agcaccagtg catcatcgct caccagcgaa cctgtactgg tcagtttccc actggccgtg
    11341 atatccactt tgcccttcgc gccagtgcgg ccgctctggg taaagtcgcg ggtattcacg
    11401 gtcaggggac cgccactgag cagggagcca ctgttgctga gcgttgtact gccgagcgtc
    11461 agggaagccc cctgaacagc accactgtta ttcagcgtgc cggcatcgag tcccgcatga
    11521 cctttcgcca gcaatattcc gtcctgtgtc agcgtggtgg cgctggccgt gagattctgt
    11581 ccggcggtta tctgtccctg tgttgtcagc gtgtcactgg cgacagtcac gatatcgcgg
    11641 gccgcgttaa tctggctggc ggtatcctgt gtgatgtttt tcgcggcaag cgttacatcc
    11701 cggccggcag tcagtttttc attctgttga gtgattctgc cgccggcggt caggctgagg
    11761 ttcttgtcgc tgttaagcgt tccattgctg agaacgatat cgctccggct gctgacgctc
    11821 aggttacctc ccgctttatg gtcgccggtt aaggtgacgc cctgaccttt tgcagtgacg
    11881 gcccccgtgg cgagactgtt gttgacagtc agtctgccgc tggcatccag ggtgatatcg
    11941 ccatcgcggg cataaaggtt accaagatta accccgacac cactttcagt ggaggtcaga
    12001 tgaatacgcc tggcgtacat tccaccgaga gcgccggtat caacggcaac tttcggcaca
    12061 ttaccttcgc ccttcagggc actgacgcga ccatctgcag ttatccggtt agcgcctgca
    12121 gtgacagtta aatccttcgc atgaagcgcg gcattcactt ccgttgcacg ggcaataatg
    12181 gatacggcat cgctccgggt gccgtccagg cccgcgccat tgatggtgat actgccttca
    12241 gtcacctcca gcgcctgcag gctgccgtcg gcattcatca caggtttgcc tgtggtgagc
    12301 gtcgcgtgcg gcgtgttgat aaaaccacag ccgtcacagg tgataccata cgggttggca
    12361 accatcacat tcgccgcttt gccggccact tccgtatagc cctgcagcag tgaacggtta
    12421 ccgccggtca cttcgttgat gatacccttc gcttccccgc ccgctttcag gttcgggtta
    12481 ttctgtatca gtccaccaag ctgcgtcgga ttaagcttac cggtggcatt attgagaatc
    12541 agcccttcct tcccgacgtt gtaatccgta aaccggttat gcgaaatccc ggccccgttc
    12601 ggcgtggcaa tgttcacgac cggcacacca tttgccgctt tatccattcc ggccccgttt
    12661 tgtggggtga tgacggcccc cacagccggt aacaacggct gcccggcgat aatcgtactg
    12721 ataaggtaac tcagcaggcg gtaagtgaag cgaacgggag gctgatgcat ggtaataatc
    12781 ccttaaaacg cgacggcaac gcgccagtaa accgtgagat ggtcaggggc aagccagtcc
    12841 gggtaaacaa gaggcagtcc ggcagtgaac gaaccggaaa catggccact ggtggtgctg
    12901 agcccggcag cagcccccca cagcgtgccg gacgagtacg ggtcatctct gtcagagtgc
    12961 agccagccac cgtccagtgc agtcactgca cggacagttc ccacatatgg cagggagaac
    13021 agagaccagg acagctcatt tcgcagataa ccaccgttat taccggagat atactgctcc
    13081 ttaaagccac gcactgaact ctcgcccccg aggctcagtt gttccacacc atgaagacgg
    13141 tccggtgacc actgggcata agcgctggtc agccaccaca ccctgtccgt gacggggcgc
    13201 tgaaaactgg cactcaccga ccatttccgg aactgattta cgggcaggtc tccccttttc
    13261 ccgtggtcgc tttctgcgcc gaaccagggc atcccccgtg tgaataccgg attcagtgtt
    13321 ccgacacccc ccagaaactt gtgtgtgtga ttcagcccga cagaaaatga agtgagttta
    13381 cggctgctgc cctgaagcag aacatcatcc agataattgt gaataatgcg gtgctgcaga
    13441 cctccggtca gtgctgtctt catgtccccg ttacggaaca ggacatgcga cagtcccagc
    13501 cggtgagtct gcaggtctcc cgtggaacgc caccgccagc cccggttatc aatggtgctg
    13561 aggtagtcac tccatgaata cgtataatcc accagggtat agccatacgg cagactgaca
    13621 ccggcggcaa aattcctcgc atcatgtgac accgaaaagt cactgctccg tcccccgctg
    13681 acaaaccagt tgtcagccag ccccagcgga ttattaaagg aaaggacacc atttaactgc
    13741 cccgtaccgg tattcttctg cccgctgttg tcgataccaa cgctccctgt gacgggccat
    13801 tccggcaatg ccgtcagtgt caccaccgac catccctcac ggtcaccggg cgatatttca
    13861 atctgtaccg gctccgtacg cagacgatta atctgctcca tcccctgctc aatatcccgc
    13921 aggttcagaa ccttcccctc cattcccggg aaaaccatct tcagggtgcg ggcaggaagg
    13981 tcagcgcctt ccgcccggat ttgctgcagc ctgccttcca tgaccgttat gtgcagtatg
    14041 ccccctgaaa ggtcctgctc tgtcagaaag gcccggctgg tgatatatcc ccggcgtata
    14101 tagccgtccg tcacggcatc cgtgaccgcg gtcagccccg tgatattcag acactgattc
    14161 acccacggtg ccaccagtct gtcggtttct gcagacgtca gtcgggtggc cccactgaca
    14221 acaatgcgtg acaccgtaaa gcaggggcct tcagtaccgg cagacgtttc cggagacggc
    14281 gtgatagtca gcggcgcact gcgctccagc gcatcacgct gacgctggtt ttcatccagc
    14341 aactgctgct gttgctgctg aattgcactg cggtcacccg gagacagcat ggctgccgga
    14401 gacgaaaatg cggggataaa aaaaccagaa aaaacggaga gggagagata gcatatccac
    14461 ggagagccgc gccggaacat atcacaacag gggacagaca acgcacggga taaaagggac
    14521 gttttcgtca gaatgttgtt ctgccggcgc tgcatgttct ccccgtattc tggcctgtgt
    14581 gatgccagtg taaattcatg tcaaaaagtg tcagcgagaa aatttacacc tgaaaaagtc
    14641 tgtcatgaaa cagttttatg acaagacatc aaaaaaatgt caaaaacgtg atctttcttc
    14701 agcttttcag aatattgagt accaggaagt caatatcaat tctgggacac atatgcattt
    14761 accattaaaa ccgggcacaa caagctgttc agtctcaatc cataccccgt gttttatcaa
    14821 tatcagaata agaatatctg cggataaata aaaaaggaat atcgagggag cttaccgcag
    14881 agggctctcc acctcacaaa ccatttacaa aaatgatacc ggcatatctt tggtccctgt
    14941 cacaaccaga cattgacaat ccaatataac cccaaatata tttcgggtaa gaagtctggg
    15001 atttatacag atatactgtc tgttattact at
//
LOCUS       DAPPNJ010000111        15027 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000111 DAPPNJ010000000
VERSION     DAPPNJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.112"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..411
                     /gene="tnpA"
                     /locus_tag="RPM73_002823"
     CDS             <1..411
                     /gene="tnpA"
                     /locus_tag="RPM73_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDX6521165.1"
                     /translation="KETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEKLRTYFSN
                     VCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAVRYY
                     YKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(427..615)
                     /locus_tag="RPM73_002824"
     CDS             complement(427..615)
                     /locus_tag="RPM73_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDX6521166.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(619..978)
                     /gene="yeaR"
                     /locus_tag="RPM73_002825"
     CDS             complement(619..978)
                     /gene="yeaR"
                     /locus_tag="RPM73_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDX6521167.1"
                     /translation="MLQILQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1151..1789)
                     /gene="leuE"
                     /locus_tag="RPM73_002826"
     CDS             complement(1151..1789)
                     /gene="leuE"
                     /locus_tag="RPM73_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDX6521168.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1916..2839)
                     /gene="dmlR"
                     /locus_tag="RPM73_002827"
     CDS             complement(1916..2839)
                     /gene="dmlR"
                     /locus_tag="RPM73_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDX6521169.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2942..4027
                     /gene="dmlA"
                     /locus_tag="RPM73_002828"
     CDS             2942..4027
                     /gene="dmlA"
                     /locus_tag="RPM73_002828"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDX6521170.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4278..5888
                     /gene="yeaV"
                     /locus_tag="RPM73_002829"
     CDS             4278..5888
                     /gene="yeaV"
                     /locus_tag="RPM73_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDX6521171.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5920..7044
                     /gene="yeaW"
                     /locus_tag="RPM73_002830"
     CDS             5920..7044
                     /gene="yeaW"
                     /locus_tag="RPM73_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDX6521172.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7100..8065
                     /gene="yeaX"
                     /locus_tag="RPM73_002831"
     CDS             7100..8065
                     /gene="yeaX"
                     /locus_tag="RPM73_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDX6521173.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8119..9234)
                     /gene="rnd"
                     /locus_tag="RPM73_002832"
     CDS             complement(8119..9234)
                     /gene="rnd"
                     /locus_tag="RPM73_002832"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDX6521174.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9316..11001)
                     /gene="fadD"
                     /locus_tag="RPM73_002833"
     CDS             complement(9316..11001)
                     /gene="fadD"
                     /locus_tag="RPM73_002833"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDX6521175.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11206..11787)
                     /gene="yeaY"
                     /locus_tag="RPM73_002834"
     CDS             complement(11206..11787)
                     /gene="yeaY"
                     /locus_tag="RPM73_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDX6521176.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11827..12522)
                     /gene="tsaB"
                     /locus_tag="RPM73_002835"
     CDS             complement(11827..12522)
                     /gene="tsaB"
                     /locus_tag="RPM73_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDX6521177.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12580..14490)
                     /locus_tag="RPM73_002836"
     CDS             complement(12580..14490)
                     /locus_tag="RPM73_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDX6521178.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSMADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14622..14966
                     /locus_tag="RPM73_002837"
     CDS             14622..14966
                     /locus_tag="RPM73_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDX6521179.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3643 a         3970 c         3721 g         3693 t
ORIGIN      
        1 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
       61 tttgtcacca gataccgact ccagattttt gaccatgacg cgacagaaaa actacgcact
      121 tacttttcaa atgtatgtgc tgattttgaa gctgaacttg ttgaaatgga tggcgaacca
      181 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac
      241 agcctcaaag gcgtatcggg taggttactg cgacgagatc gaccagatat tgcagtcagg
      301 tattactaca aaggcgtttt gtggagtcct ggctattttg ccagtagctg cggaggtgcg
      361 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtta ggtggaaaac
      421 cgcgccttat atccccgccc tgaaggacgg ggttttacgg cgcaccggat aagaacgcac
      481 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
      541 cgtctctgtt tcataatcga cagaaacgcc attgctgtta ttggtgacgg tcacggtata
      601 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
      661 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
      721 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
      781 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
      841 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
      901 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
      961 ctgtaggatt tgcagcatcc tattccctcc ttcggggaat gcgctgaaaa cggtttattc
     1021 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     1081 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     1141 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     1201 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     1261 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     1321 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     1381 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     1441 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     1501 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     1561 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     1621 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     1681 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     1741 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     1801 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     1861 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaatc attaatcagg
     1921 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
     1981 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     2041 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     2101 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     2161 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     2221 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     2281 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     2341 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     2401 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     2461 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     2521 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     2581 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     2641 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     2701 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     2761 ccgccacggc ggcaaaacca gcccgacgag ccaccagcat aaagacgcgc aaatcattca
     2821 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     2881 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     2941 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
     3001 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg ccttgagttt
     3061 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
     3121 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
     3181 ggacaccgtt cctgaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
     3241 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
     3301 gggaaaacag ccaggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
     3361 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
     3421 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
     3481 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
     3541 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
     3601 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
     3661 ggtggcgtcc aatttatttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
     3721 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
     3781 gccagtccac ggttccgcgc cggatatcta cgggaaaaac attgctaacc ctatcgccac
     3841 gatttgggcc ggtgcaatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
     3901 agcgcataac ggtattctgg cagcaattga agaagtgatt gctcacgggc cgaaaacacc
     3961 tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
     4021 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
     4081 tttgcagtaa ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
     4141 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
     4201 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
     4261 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
     4321 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
     4381 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
     4441 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
     4501 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
     4561 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
     4621 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
     4681 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
     4741 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
     4801 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
     4861 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
     4921 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
     4981 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
     5041 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggcc
     5101 caaccgaatt tattaccaac agcatcatca atgccatcgg tctgaccacg caaaacttcc
     5161 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
     5221 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
     5281 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
     5341 gttgctggtt cttctttggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
     5401 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgtg cagcaagttc
     5461 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggtgtg atgattattt
     5521 tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt acgcgtaatc
     5581 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
     5641 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
     5701 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
     5761 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
     5821 caccggttga agccctggaa aaaacgccag tgctccctgc gggaaccgta ttcaaaggcg
     5881 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
     5941 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
     6001 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
     6061 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
     6121 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
     6181 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
     6241 acctgcccgt atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
     6301 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
     6361 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
     6421 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
     6481 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
     6541 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
     6601 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
     6661 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
     6721 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
     6781 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
     6841 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
     6901 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
     6961 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
     7021 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
     7081 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
     7141 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
     7201 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
     7261 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
     7321 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
     7381 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
     7441 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagct
     7501 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
     7561 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcatc
     7621 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
     7681 ttatacctgt ggccccgagg cgctaattga agcggtaaga agtgaagctg cgcgtctgga
     7741 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
     7801 atttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
     7861 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
     7921 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcaatattt
     7981 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
     8041 taaacgcctg gtgttggatt tgtagtttgc cgaagccgga tgtggcgctg agcgcgccca
     8101 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
     8161 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
     8221 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
     8281 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
     8341 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
     8401 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
     8461 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgctaccg accacaaatg
     8521 ctcttcacgc acgacaaagt tcaccgccag atcgcgctct cgcgccttgc gcagtcgcca
     8581 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
     8641 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
     8701 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
     8761 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
     8821 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
     8881 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
     8941 gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
     9001 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
     9061 gtcggtgatc ccgagaggat caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
     9121 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
     9181 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
     9241 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
     9301 cgactgactt aacactcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
     9361 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
     9421 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
     9481 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
     9541 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
     9601 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
     9661 gaacccttct tcatccatca ccgcaatgtc gccggtgtgt aaccagccat ttttgatgat
     9721 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
     9781 aagctcaccc ggttgacccg gtggtacttc attatcatca tcatccacca gtttggcttc
     9841 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
     9901 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
     9961 tttcacccaa cgctctgcca ccacttgctg cactggcatc cctccgcctg cggaaagatg
    10021 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    10081 gttaacgccc gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    10141 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    10201 aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc accaccagct ctttgcccgg
    10261 atgcaacagc ggaccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    10321 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    10381 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    10441 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    10501 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    10561 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    10621 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    10681 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    10741 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    10801 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    10861 ctcccccata ttcacaaacg caggctgatc ggcgtagcgc gcgaccgact gctcaaacat
    10921 atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg cgggataacg
    10981 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    11041 cccaaatgat atacatgccg ttaacataat attaactcat cataccagct tgataattac
    11101 ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt aagaatgcag
    11161 aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt cagttacaac
    11221 tgtttgtact ctcgcggggc cgggattata ccagccccat ccgccatagc catagggcca
    11281 gccacgaccg ccataaaacc atggatcaat cggctgaggc ggcataatca cctgctgggt
    11341 taaatgccaa cgtttgtaac ccgttacttg catcaccata aatttatagg gcgtgttgcc
    11401 gatttttccg tcaaccgcac cagtgattgg cccgactacc gtaaccagtt gtccacggaa
    11461 atccaccggg tccagaaaac cgttcatatc ggcataaatg cgaccgcgag aaggttctcc
    11521 cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    11581 ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac caacgtacag
    11641 ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc tgcctttaat
    11701 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    11761 tttgataaca ttcttttgaa ccgccatggt gcgactcctt tttttctcag ggcatactct
    11821 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    11881 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    11941 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    12001 accgagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    12061 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    12121 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    12181 ggctgacagc acgcgggttg cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag
    12241 cgtggagacg ccaatcatcg gtaattccgc gccaagcgcc agcccttgcg cgatgccaat
    12301 accaatgcgc acgccggtaa agctaccggg gccgcgcccg taagccagag cgtttatgtc
    12361 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    12421 atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc acagggcgac
    12481 agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgctttttt
    12541 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    12601 aaggaaacga accgcacggg caatgtcacg agtgcgtggc gcgggcggca gactggcgag
    12661 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaatcacca gcacgccacg
    12721 atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg agagtaatga cggcatctgg
    12781 tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    12841 taacagtgga tcatccggcg aggtaaacgg caacttgtcg ataatcacca atgacaatgt
    12901 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    12961 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    13021 aagcgtcatg gtagcgcgga actgctcggc cagatcacgc atcatggcgt gcgaggtaca
    13081 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgctaactg
    13141 gcgagcagaa cctggctggt tggtttgcgg cagattgcgc ggcacacaga gtaacgcctg
    13201 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    13261 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    13321 accaggcttt tgcgccatta actctttgaa tttatccgcc atgctgagcg gcgtgagagc
    13381 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    13441 ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc tcaaatgccg catccagcaa
    13501 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    13561 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    13621 acctggctca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca
    13681 cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa gcgatggtga tgtcttttgc
    13741 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    13801 tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcagtt cgccaaatcc
    13861 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    13921 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    13981 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    14041 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    14101 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    14161 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    14221 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    14281 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    14341 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    14401 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    14461 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    14521 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    14581 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    14641 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    14701 taccggaaaa cctcgacgcc gatgcttttg agcaaaccgc caacacgctg gcgcagattg
    14761 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    14821 tggctgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtggcgg
    14881 gtcatgcgcc ggtgcgctgc acggtgcaag cggggctgat gaacccgaag tataaagttg
    14941 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    15001 tatcgataat actggtcggt tttacat
//
LOCUS       DAPPNJ010000112        14996 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000112 DAPPNJ010000000
VERSION     DAPPNJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.113"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(75..2354)
                     /gene="maeB"
                     /locus_tag="RPM73_002838"
     CDS             complement(75..2354)
                     /gene="maeB"
                     /locus_tag="RPM73_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDX6521180.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2643..3593
                     /gene="tal"
                     /locus_tag="RPM73_002839"
     CDS             2643..3593
                     /gene="tal"
                     /locus_tag="RPM73_002839"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDX6521181.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3613..5616
                     /gene="tkt"
                     /locus_tag="RPM73_002840"
     CDS             3613..5616
                     /gene="tkt"
                     /locus_tag="RPM73_002840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDX6521182.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5712..6755)
                     /locus_tag="RPM73_002841"
     CDS             complement(5712..6755)
                     /locus_tag="RPM73_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDX6521183.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6881..7456)
                     /gene="nudK"
                     /locus_tag="RPM73_002842"
     CDS             complement(6881..7456)
                     /gene="nudK"
                     /locus_tag="RPM73_002842"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDX6521184.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7524..9503)
                     /gene="aegA"
                     /locus_tag="RPM73_002843"
     CDS             complement(7524..9503)
                     /gene="aegA"
                     /locus_tag="RPM73_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDX6521185.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9709..11409
                     /gene="narQ"
                     /locus_tag="RPM73_002844"
     CDS             9709..11409
                     /gene="narQ"
                     /locus_tag="RPM73_002844"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDX6521186.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11573..14686
                     /gene="acrD"
                     /locus_tag="RPM73_002845"
     CDS             11573..14686
                     /gene="acrD"
                     /locus_tag="RPM73_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDX6521187.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLNPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14785..14844)
                     /gene="ypfM"
                     /locus_tag="RPM73_002846"
     CDS             complement(14785..14844)
                     /gene="ypfM"
                     /locus_tag="RPM73_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDX6521188.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3513 a         3988 c         3917 g         3578 t
ORIGIN      
        1 taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg gtaagcgtga
       61 gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag cgccaccatg
      121 ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt cgccacgccc
      181 atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa gttgtaacta
      241 atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt caaagggctg
      301 tccggcatac ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg catttcacca
      361 tcaatcatca gatctggcgc acgatccctg accagttcca gcgcctgacg cattttgctc
      421 gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac gcgcggctca
      481 ataccaaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag ctcttctgcg
      541 tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg cagcagcagc
      601 gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt tttcaccacg
      661 ctaaaatgtt catgataatc gcccaccgta ccgcagatca ttgcatcggc ttccccacgc
      721 tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg cgcctgttcc
      781 tgagtgacac cgcgacgctt catgatctgg aagtattcgg tccagtactc tttaaagcgc
      841 ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg caagcccagt
      901 ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt cgccagtccc
      961 agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc tggcagaaca
     1021 acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag gttggttttg
     1081 taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg acgcgtcgcc
     1141 acgcccgact ccatcgcggc tttagcgacc gcaggagcga tcttaacgat caagcgcgga
     1201 tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc gccatacgct
     1261 gaagccacca cttcgctctg ttcggcgtgg gccagttccg caatcgcgcg taccgccgcc
     1321 agtttcatct cttcattgat agccgttgcg ccaacatcca gcgcgccacg gaagataaac
     1381 gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt gcaaatgatg
     1441 gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg gttcgccagc
     1501 gccaggatca ttggcgcacg agccattttc ttcaccattt cctgggtcag cactttcggg
     1561 ccggaacatc caaggaaaat atccgcgcct tcaatcacat catcgagggt acgtttgccg
     1621 tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg gccctgatag
     1681 ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag cgctaccagc
     1741 aggttcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg cacgtcggag
     1801 atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat gattgccgtg
     1861 ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt ctgttcaata
     1921 tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt cggttcgagc
     1981 gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat gtcaaataca
     2041 tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg tttgccagcc
     2101 agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac cgccaccagg
     2161 ttaccgcgcg cggtatattt gtaggctttt aacgggtctt tttcgatttc aagacaaggt
     2221 gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct gcgttgccag aggcttggtt
     2281 ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc actttgtttt
     2341 aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag tgtgtgggca
     2401 acagcccctg cccacaacgt ggcgcacatt attaccctgc cggagtcttc agactttgag
     2461 caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa cactgatgtt
     2521 acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc gtttagcgaa
     2581 atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa ggagtatttc
     2641 ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac agcggcgata
     2701 ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg ctgttactca
     2761 aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc tggggtaaaa
     2821 aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg gcggtcaatt
     2881 tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt gatgcacgcc
     2941 tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac ttgtatcagc
     3001 aacaaggcgt tgagaaatca cgcattttga tcaaactggc ttcgacctgg gaaggcattc
     3061 gcgcggccga agagctggaa aaagaaggta ttaactgcaa cctgacgctg ctgttttctt
     3121 ttgcccaggc gcgggcctgt gcagaagcgg gcgtttttct gatttcgccg tttgtcgggc
     3181 gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg gaagaagatc
     3241 cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac tatgaaacca
     3301 ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc ggctgcgatc
     3361 gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg ccagtggtac
     3421 gtaaattaat cccgccttct cagacgttcc cacgcccagc acccatgagc gaagcggagt
     3481 tccgttggga gcacaatcag gatgcgatgg cggtagaaaa actgtctgaa ggcattcgtc
     3541 tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta taaaccagcc
     3601 acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact cagtatggat
     3661 gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc tgatattgcc
     3721 gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg gtatgatcgc
     3781 gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt gctacatctg
     3841 accggttacg acctgccgct ggaagaactg aagaacttcc gtcagttgca ttcgaaaacc
     3901 ccaggccacc cggagattgg ctatacgcca ggcgttgaaa ccaccaccgg cccgcttgga
     3961 caaggtttgg cgaacgccgt cgggctggcg atagcggaac gtacattggc ggcgcagttt
     4021 aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg cgacggctgc
     4081 ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg actgggcaag
     4141 ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga aggctggttt
     4201 accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca tgaaatcgac
     4261 ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt gaaagataag
     4321 ccgtcgctga ttatctgccg tacggtgatt ggctttggtt cgccgaataa agcaggtaag
     4381 gaagaggcgc acggcgcacc actgggggaa gaagaagtgg cgctggcacg gcaaaaactg
     4441 ggctggcacc atccgccatt tgagatccct aaaaagattt atcacgcctg ggatgcccgt
     4501 gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt ttgccgccta taaaaaggct
     4561 catccgcaac tggcagaaga gtttacccga cggatgagcg gtggtttacc gaaggactgg
     4621 gagaaaacga ctcagaaata tatcaatgag ttacaggcaa atccggcgaa aatcgctacc
     4681 cgtaaggctt cgcaaaatac gcttaacgct tacgggccga tgctgcctga gttgctcggc
     4741 ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt ttcgctgaag
     4801 gaagatccag cgggcaacta cattcactac ggggtgcgtg aatttggcat gaccgctatc
     4861 gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt cctgatgttt
     4921 gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg gcagattatg
     4981 gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca ggctgttgag
     5041 caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg cgatcaggtg
     5101 gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac ggcactgatc
     5161 ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa agagattgct
     5221 cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattatttt gattgccacc
     5281 ggttcagaga tggaaattac cctgcaagcg gcagagaaat tagcaggaga aggtcgcaat
     5341 gtacgcgtag tttccctgcc ctcgaccgat attttcgacg cccaggatga ggaatatcgg
     5401 gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg tattgccgat
     5461 tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg ttacggggaa
     5521 tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa tattgtggca
     5581 aaagcgcata aggtgctggg agtgaaaggt gcctgatggt gatcgccgga tgctgattgc
     5641 cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc tacatgtccc
     5701 cgcaattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc atgccagtta
     5761 tcgcaggtgg gttctgcggc ataacgcacc aggcgaaaac gctgaccgtc aaagcgccag
     5821 cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac cagttcacgc
     5881 gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg accgttgttg
     5941 aacggcaaac gcaaccgaac cgggcgcgag gctagtggct ttttacgcga cacaatccat
     6001 gccaaatcaa tggtgttata ggcccctgcc tcacagctaa tcatcatcag cgctttatca
     6061 tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca gcgcagacca
     6121 ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga gagtggcgtc
     6181 ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac gctgagcggc
     6241 ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca
     6301 ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc gtcgcgtaag
     6361 gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc cgtatcatcg
     6421 gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa cggctcacca
     6481 tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt caacccgccg
     6541 cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag ggtcatcacc
     6601 agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt acaggtgacc
     6661 tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt cggcaacaaa
     6721 gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa
     6781 acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca gatttgtcgg
     6841 attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt aaatgtgacg
     6901 tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca
     6961 tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc
     7021 caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag
     7081 tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc
     7141 ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc
     7201 cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga
     7261 actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc gtcgcaccat
     7321 taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat
     7381 cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg
     7441 tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt acgctgtgaa
     7501 gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtca
     7561 ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca
     7621 cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg gtactggctt
     7681 tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac
     7741 ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg catgacaaat
     7801 tcactgccct caatcggtac aggtcgccga cgcccctggg catccggctc gccaagacgc
     7861 gtgcgcagga aacgaatccc gcagacgtga ccttgttcat tcagctcaag cgccaccggc
     7921 tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcctcacctc tttcttcgag
     7981 cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc
     8041 agcgcagtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt
     8101 ccggcggtat tgataaacgg ctcttccggt agctcttcga gccccatcac ctgtttagtg
     8161 ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc
     8221 gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc
     8281 gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg
     8341 ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg
     8401 aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcacccc cacgccattg
     8461 cgggtcagaa catccgcaca ggccagccct gccggacctg caccgataat cgccacccgc
     8521 ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa cgcctgatct
     8581 gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg aatagtacag
     8641 gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg
     8701 gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac
     8761 tgcggtatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgcactta
     8821 aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca
     8881 aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc
     8941 tgcatttgtt cgactttact cattaccggc atttcttgcg ccgcggtact ggcatgccac
     9001 ggttgatgtt cctgacgcgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg
     9061 acgtcagtga ccagttgcag cgcgtccgcc gggcaattct caacacaggc aggaccgttt
     9121 tcgcgccccg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg
     9181 ggtgtcagga cgatttgcat cgtaccaaaa ggacaggcca ccacgcagga tttacagcca
     9241 atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg
     9301 cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgt
     9361 tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca
     9421 tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg
     9481 ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt
     9541 tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat gtgcccagaa
     9601 taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact gactaaattg
     9661 tgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa
     9721 cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt
     9781 tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat
     9841 attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca aagtggcagt
     9901 ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc
     9961 aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc
    10021 aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat
    10081 attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa
    10141 cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg
    10201 gtctttttta ctttgcgccg catacgccat caggtggttg ccccgctgaa tcagctggtt
    10261 accgccagtc agcgtattga acacggacag ttcgactcgc cgccgctgga taccagtctg
    10321 ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga gctgcataaa
    10381 ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgaggccaag
    10441 cgtcgtctgg aggtgttgta tcaatgttcc caggcgctga acaccagcca gattgatgtg
    10501 cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga atatctggag
    10561 ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga attgccgatg
    10621 cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg gcaaaatagt
    10681 cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg acgcggtttg
    10741 tactttaatc aggcgcagaa gcattttcag cagttattgt tgatggaaga acgtgcaacc
    10801 atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg tatccagttg
    10861 acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat catggccgat
    10921 ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac tactttccgc
    10981 ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga tacgttacaa
    11041 aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc gctggatgcg
    11101 caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc gatgaagcac
    11161 gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa tcacacggtt
    11221 tatatccgtg ataacgggat tggcatcggt gaaccgaaag agcccgaagg tcattatggt
    11281 ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt ttcacaacct
    11341 tccggcggcg gcacgttagt gagtattagc tttcgctccg cggagggtga ggaaagtcag
    11401 ctgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag tataaaaggg
    11461 catgataatt tacattaact cctttttttc tccacgattg gctcgtacct tgccgctaca
    11521 gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt taatggcgaa
    11581 tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt gtctgacagg
    11641 taccctggcg attttctcat tgcccgttga acaatacccc gatctcgcgc caccgaatgt
    11701 gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca ccgtgaccca
    11761 ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat ctcagagcag
    11821 tggcaccggt caggcatctg tcactttaag ttttaaagca ggcaccgatc cggacgaagc
    11881 cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc aggcggtgca
    11941 aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca ttgctttcgt
    12001 ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca gtaatattca
    12061 ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt cgcaatattc
    12121 catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg ctaaagatgt
    12181 cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg gtggtacacc
    12241 ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc tgcaaacacc
    12301 agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg taaggctggg
    12361 cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc gcttcaatgg
    12421 taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa tggcgacagc
    12481 ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg gactggaata
    12541 caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg tggtgaaaac
    12601 gctgctggaa gctatcgctc tggttttcct cgttatgtat ctgttcctgc aaaacttccg
    12661 cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct tctccgtact
    12721 ttacgccttt ggttacagcg tcaacacctt aaccatgttc gcgatggtgc tggcgatcgg
    12781 tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga
    12841 aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt
    12901 cgggattgcg atggttcttt cggcggtatt tgtaccaatg gccttcttcg gcggcaccac
    12961 cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc tgtcagtact
    13021 ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaatccac tgaaaaaagg
    13081 tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta accgcaacgc
    13141 cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat
    13201 ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt
    13261 accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg gttcaaccca
    13321 acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc atgaaaaaga
    13381 caacatcatg tcggtgtttg ccactgttgg ttctggcccc gggggtaacg ggcaaaacgt
    13441 ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga ccggcacctc
    13501 gtttgccatt atcgagcgtg caacaaaggc gtttaacaag attaaagaag ctcgcgttat
    13561 cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg atatggagtt
    13621 gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc agttgctggc
    13681 gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg acgacagtcc
    13741 gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg atatcgacga
    13801 tattaacgac acactgcaaa ccgcctgggg ttcgagctat gttaatgact ttatggatcg
    13861 cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc tgccagatga
    13921 cattaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct ctgctttcgc
    13981 gacctcacgc tgggagacag gctcgccgcg tctggaacgc tataacggtt attctgcggt
    14041 tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg atattatgga
    14101 atcgttagtg aagcagttgc caaacggctt tggtctggag tggacggcga tgtcgtatca
    14161 ggagcggctt tccggcgcgc aggctccggc gctgtacgcc atttccttgc tggtagtatt
    14221 cctgtgtctg gcggcattgt atgaaagctg gtcggtaccg ttctcggtaa tgctggtcgt
    14281 gccgctgggg gttatcggcg cgctgctggc aacctggatg cgcgggctgg aaaacgacgt
    14341 ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg ccatcctgat
    14401 cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag cgacgctcca
    14461 cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta tcttcggcgt
    14521 attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg tgggtactgg
    14581 cgtaatgggc gggatgattt cagctactat tctggctatt tacttcgtgc cgctgttctt
    14641 tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg gaataagcaa taaaaaaggc
    14701 gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca gcgggttctt
    14761 gctgtttata ctttgtctcg ggaattactt acgaagcata acttcgataa agtctttcca
    14821 gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta ttctacggaa
    14881 acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct ccgggggaca
    14941 aaccttatgt aaatactatg gtcctgcggt gataatttgt atgtgataca cagcaa
//
LOCUS       DAPPNJ010000113        14939 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000113 DAPPNJ010000000
VERSION     DAPPNJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.114"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(218..274)
                     /locus_tag="RPM73_002847"
     CDS             complement(218..274)
                     /locus_tag="RPM73_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX6521189.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            553..1800
                     /gene="mdtA"
                     /locus_tag="RPM73_002848"
     CDS             553..1800
                     /gene="mdtA"
                     /locus_tag="RPM73_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDX6521190.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLIGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            1800..4922
                     /gene="mdtB"
                     /locus_tag="RPM73_002849"
     CDS             1800..4922
                     /gene="mdtB"
                     /locus_tag="RPM73_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDX6521191.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            4923..8000
                     /gene="mdtC"
                     /locus_tag="RPM73_002850"
     CDS             4923..8000
                     /gene="mdtC"
                     /locus_tag="RPM73_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDX6521192.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8001..9416
                     /locus_tag="RPM73_002851"
     CDS             8001..9416
                     /locus_tag="RPM73_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6521193.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIDGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9413..10816
                     /gene="baeS"
                     /locus_tag="RPM73_002852"
     CDS             9413..10816
                     /gene="baeS"
                     /locus_tag="RPM73_002852"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDX6521194.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10813..11535
                     /gene="baeR"
                     /locus_tag="RPM73_002853"
     CDS             10813..11535
                     /gene="baeR"
                     /locus_tag="RPM73_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDX6521195.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGIDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11726..12058
                     /locus_tag="RPM73_002854"
     CDS             11726..12058
                     /locus_tag="RPM73_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDX6521196.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12267..12563
                     /locus_tag="RPM73_002855"
     CDS             12267..12563
                     /locus_tag="RPM73_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDX6521197.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            12565..12861
                     /locus_tag="RPM73_002856"
     CDS             12565..12861
                     /locus_tag="RPM73_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX6521198.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            12964..14325
                     /gene="yegQ"
                     /locus_tag="RPM73_002857"
     CDS             12964..14325
                     /gene="yegQ"
                     /locus_tag="RPM73_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDX6521199.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14655..>14939)
                     /locus_tag="RPM73_002858"
     CDS             complement(14655..>14939)
                     /locus_tag="RPM73_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521200.1"
                     /translation="IFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLA
                     RGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         3359 a         3987 c         4035 g         3558 t
ORIGIN      
        1 tcccgctgaa atcgtcggct tacctccttt cgccatgcac ttagtctatc gctgacttgc
       61 agataaaata gtttccagtg tgattattgc gtaacgtctc gcaaagaatt tttgctggca
      121 ggatcgcaga cggcaaagcc tgcggattga caatcttatc gtgaaggcat actttcagga
      181 gtgagggtag agcggggttt cccccgccct ggtagtctta gtatgctggg tagctaatca
      241 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttattt aaggcccctt
      301 cctcgggagg ggctttcccg tttcagcatc cggctgaaat ctgcggctta cctcctttcg
      361 ccctgtatgt agtctatcgc taacgcgtag ataaaatagt ttcctgtgtt attactggat
      421 gcgtgctcgc aaatgtgccc gccattcaga cgattcccga tagtgtttca taattcctcc
      481 atttttctcc cttattggct ggctacacta gtatcattcc gcgaaacgtt tcaggaagag
      541 aaactcttaa cgatgaaagg cagttataaa tcccgttggg taatcgtaat cgtggtggtt
      601 atcgccgcca tcgccgcatt ctggttctgg caaggccgca atgactccca gagtgcagcc
      661 ccaggggcga cgaaacaagc gcagcaatcg ccagcgggtg gtcgccgtgg tatgcgttcc
      721 ggcccattag ccccggttca ggcggcgacc gccgtagaac aggcagttcc gcgttacctc
      781 atcgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtggacggt
      841 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      901 gaaattgacc ccagccagtt caaagttgca ttagcacaag cccagggcca actggcaaaa
      961 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
     1021 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
     1081 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agctgcaact cgactggagc
     1141 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aggttgatgt tggtaaccaa
     1201 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
     1261 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
     1321 ccgctggtgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
     1381 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
     1441 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
     1501 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
     1561 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
     1621 caggacagtc agaaagtggt gatccgtgca ggtatttctg cgggcgatcg cgtggtgaca
     1681 gacggcattg atcgcctgac cgaaggggcg aaagtggaag tggtggaagc ccagagcgcc
     1741 accactccgg aagagaaagc caccagccgc gaatacgcga aaaaaggagc acgctcctga
     1801 tgcaggtgtt acccccgagc agcacaggcg gcccgtcgcg cctttttatt atgcgtcctg
     1861 tcgccaccac gctgctgatg gtggcgatct tactcgccgg gattatcggt tatcgcgccc
     1921 tgcccgtttc ggcgctgccg gaagtggact atccgaccat tcaggtggtc acgctctacc
     1981 caggtgccag cccggatgtc atgacctctg ccgttaccgc gccgctagaa cgccagttcg
     2041 ggcagatgtc tggcctgaaa cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca
     2101 ctttgcagtt ccagctaaca ttaccgctcg atgtcgccga gcaggaagtg caggccgcga
     2161 ttaacgctgc gaccaacttg ttgccgagcg atctgcctaa cccgccggtt tacagcaaag
     2221 tgaacccggc agatccgccg atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga
     2281 cgcaagtgga agatatggtg gaaacccgcg tcgcgcagaa aatctcgcag atttccggtg
     2341 tcggactggt gacgctttcc ggcggtcagc gtccggctgt tcgcgtcaaa cttaacgctc
     2401 aggcgattgc cgccctcggc ctgaccagcg aaaccgtgcg caccgccatt accggcgcta
     2461 acgttaactc ggcaaaaggt agcctcgacg gcccttcccg tgcggttacg ctttccgcta
     2521 acgaccagat gcaatccgcc gaagagtatc gccagctaat catcgcctac cagaacggcg
     2581 cgccaattcg tctgggcgat gtcgcaactg tagagcaagg tgcagaaaac agctggctcg
     2641 gcgcgtgggc gaacaaagaa caggccattg tgatgaatgt tcagcgccag cccggtgcta
     2701 acattatctc caccgccgac agcattcggc agatgctgcc acagctcact gagagtctgc
     2761 cgaaatcggt gaaggtgaca gtgctttccg atcgcaccac caatatccgc gcatccgtcg
     2821 atgatactca gtttgaattg atgatggcta tcgcgctggt agtcatgatt atctacctgt
     2881 ttttgcgcaa tattccggcg accatcattc ccggtgttgc tgtaccgctg tcgttaatcg
     2941 gcactttcgc ggttatggtg tttctcgatt tttcaatcaa taacctgaca ctgatggcgt
     3001 taactatcgc caccggattc gtggtcgatg acgccatcgt agtgatcgaa aacatttccc
     3061 gctatatcga aaaaggcgaa aaaccgttgg cggcggcgct caaaggcgcg ggtgaaatcg
     3121 gctttaccat tatctcgctg accttctcgc tgattgcggt gttgattcca ctgctgttta
     3181 tgggcgatat cgtcgggcga ctgttccgcg aatttgctat taccctggcg gtagcgattt
     3241 tgatctcagc ggtggtgtcg ctgaccctga caccgatgat gtgcgcgcgg atgctcagcc
     3301 aggagtcgct gcgtaaacag aaccgattct cccgtgcctc ggaaaaaatg ttcgacagga
     3361 taatcgccgc ctatggtcgt ggactggcga aggtgctgaa tcatccgtgg ctgaccttaa
     3421 gcgtggcgct cagcacgctg ctgcttagcg ttctcttatg ggtgttcatt ccgaaaggtt
     3481 tcttcccggt acaggacaac ggcattattc agggcacttt gcaggcaccg caatccagct
     3541 cctttgccaa tatggcccag cgacaacgcc aggtcgcgga cgtgattttg caggatccgg
     3601 cagtgcaaag cctgacctca tttgttggcg ttgatggcac taacccgtcg ctgaatagtg
     3661 cacgtttaca aatcaacctc aaaccgctgg atgaacgtga tgaccgagtg caaaaagtca
     3721 ttgcccgtct gcaaacggcg gtggataaag tgccgggcgt cgatctcttc ctgcaaccaa
     3781 cgcaggatct gactattgat actcaggtca gccgcaccca gtaccagttt accttgcagg
     3841 ccacctcact ggatgcgctc agtacctggg tgccacagtt gatggaaaaa ctccagcaac
     3901 tgccacagct ttctgatgtc tccagcgact ggcaggacaa agggctggtg gcgtatgtca
     3961 atgttgatcg cgacagcgcc agccgtctgg ggatcagcat ggcggatgtc gataacgccc
     4021 tgtacaacgc gtttggtcag cgactgattt ccactattta tactcaggct aaccagtatc
     4081 gcgtagtgct ggagcacaac accgaaatta ccccgggcct cgcggcgctg gataccattc
     4141 gcctgaccag tagcgacggc ggcgtggtgc cgttaagctc aattgccaaa gttgaacagc
     4201 gttttgcgcc gctttccatc aaccatctgg atcagttccc ggtaacgacc atctccttta
     4261 acgtgccgga taactattcg ctgggcgatg cggtgcaggc gattatggac accgaaaaga
     4321 cgctgaatct gccggtggat atcaccacgc agttccaggg cagcaccctc gccttccagt
     4381 cggcgctggg cagcactgtc tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc
     4441 tcggcattct gtacgagagc tttattcacc cgatcaccat tctctcgacg ctacccaccg
     4501 caggggttgg cgcactgctg gcgttgctga ttgctggtag cgaactggat gtgattgcga
     4561 ttatcggcat tattttgctg atcggtatcg tgaagaagaa cgccatcatg atgatcgact
     4621 tcgcgctggc tgccgagcgc gagcaaggca tgtcgccgcg cgaggcaatc taccaggctt
     4681 gtctgttgcg ttttcgtccg atcctgatga ccactctggc ggctctgctt ggcgcgctgc
     4741 cgctgatgtt gagtaccggg gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg
     4801 tcggcggcct gattgtcagc caggtgctga cgctgtttac cacgccggtg atttatctgc
     4861 tgttcgaccg cctggcattg tggaccaaaa gtcgctttgc tcgccatgaa gaggaggcgt
     4921 aagtgaagtt ttttgccctc ttcatttacc gtccggtggc gacgatttta ctgtcggttg
     4981 ccattaccct gtgcggcata ctgggcttcc gtatgctgcc ggtcgccccg ctgccgcagg
     5041 tcgattttcc ggtgattatg gtcagcgcct cgctgcccgg tgcgtcacca gaaacaatgg
     5101 cgtcttccgt tgccacgccg ctggagcgct cacttgggcg cattgccgga gtcagtgaaa
     5161 tgacctccag cagttcgctc ggcagcacgc gtattatttt gcagtttgat tttgaccggg
     5221 atatcaacgg cgcggcgcgc gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc
     5281 ccagcgggat gcccagccgt ccgacctatc gcaaagctaa cccgtcggat gcgccaatta
     5341 tgatcctcac gctgacatcc gatacttatt cgcagggtga actgtacgat ttcgcctcga
     5401 cgcagctggc tccgacgatt tcgcaaatcg acggtgttgg tgatgtcgat gtcggcggca
     5461 gctcactgcc cgccgtacgc gtcgggctga atccgcaggc gctgtttaat cagggcgtct
     5521 cgctggacga cgtacgcact gccatcagca atgccaacgt gcgtaaaccg cagggggcgc
     5581 tggaagatgg cactcaccgc tggcagatcc agaccaatga tgagctaaaa accgccgccg
     5641 aatatcagcc gctgattatt cattacaaca acggcggcgc ggttcgtctg ggcgatgtgg
     5701 cgacagtgac cgactctgta caggatgtac gtaacgccgg gatgaccaat gccaaaccgg
     5761 ctattttgct gatgatccgc aagctgccgg aagccaatat tatccagacg gttgacagca
     5821 tccgggcaaa attaccggag ttgcaggaaa ccattccggc ggcgattgat ctgcaaattg
     5881 ctcaggatcg ctcccccacc attcgcgcct cgctggaaga agtcgagcaa acgctgatta
     5941 tctcggtggc gctggtgatt ctggtggtgt ttttattcct gcgctcgggt cgcgcaacta
     6001 ttattcccgc cgttgcggtg ccggtttcgc tgattggtac gtttgcggcg atgtacctgt
     6061 gcggtttcag tctcaataat ctttcgttaa tggcgctcac catcgctact ggtttcgtgg
     6121 tggatgacgc catcgtggtg ctggaaaaca ttgcccgtca tctggaagcg ggaatgaaac
     6181 cgttgcaagc cgcactgcaa ggtactcgcg aagtcggttt tacggtgctg tcgatgagtc
     6241 tgtcactggt ggcggtgttc ctgccgctgc tgttgatggg cggattgccg ggccgactgt
     6301 tacgcgaatt tgccgtgacg ctttctgtcg ccattggtat ttcgttgctg gtttctctga
     6361 cattaacgcc aatgatgtgt ggctggatgc tgaaagccag caagccgcgc gagcaaaagc
     6421 gactgcgtgg ttttggtcgc atgttggtag ccctgcaaca aggctacggc aagtcactga
     6481 aatgggtgct caatcatacc cgtctggtgg gcgtggtgct gcttggcacc attgcgctga
     6541 atatctggct gtatatctcg atcccgaaaa ccttcttccc ggagcaggac actggcgtgt
     6601 tgatgggcgg gattcaggcg gatcagagta tttcgtttca ggcgatgcgc ggtaagttgc
     6661 aggatttcat gaaaattatc cgtgacgatc cggcagtgga taatgtcacc ggctttacag
     6721 gcggttcgcg agtgaacagc gggatgatgt ttatcaccct caagccacgc gacgaacgca
     6781 gcgaaacggc gcagcaaatt atcgaccgtc tgcgcgtaaa actggcgaaa gaaccggggg
     6841 cgaatctgtt cctgatggcg gtacaggata ttcgcgttgg tgggcgtcag tcgaacgcca
     6901 gctaccagta cacgttgtta tccgacgacc tggcggcact gcgcgaatgg gagccgaaaa
     6961 tccgcaaaaa actggcgacg ttgccggaac tggcggacgt gaactccgat cagcaggata
     7021 acggcgcgga gatgaatctg gtttacgacc gcgacaccat ggcacggctg ggaatcgacg
     7081 tacaagccgc caacagtctg ttaaataacg ccttcggtca gcggcaaatc tcgaccattt
     7141 accagccgat gaaccagtat aaagtggtga tggaagtgga tccgcgctat acccaggaca
     7201 tcagtgcgct ggaaaaaatg ttcgttatca ataacgaagg caaagcgatc ccgctgtcgt
     7261 atttcgctaa atggcaaccg gcgaatgccc cactatcggt gaatcatcag ggattatcgg
     7321 cggcctcgac catttcgttt aacctgccga ccggaaaatc gctctcggac gccagtgcgg
     7381 cgatcgatcg cgcaatgacc cagcttgggg tgccttcgac ggtgcgcggc agttttgccg
     7441 gcacggcgca ggtgttccag gagacgatga actcgcaggt gatcctgatt atcgccgcca
     7501 tcgccacggt gtatatcgtg ctgggtatcc tttacgagag ttacgtacat ccgctgacga
     7561 ttctctccac cctgccctcg gcgggcgttg gagcgctgtt ggcgctggag ctgttcaatg
     7621 ccccgttcag cctaatcgcc ctgataggga tcatgctatt aatcggcatc gtgaagaaaa
     7681 acgccattat gatggtcgat tttgcgcttg aagcccaacg gcacggtaac ctgacgccgc
     7741 aggaagctat tttccaggcc tgtctgctgc gttttcgccc gattatgatg actaccctgg
     7801 cggcgctgtt tggtgcgctg ccgctggtat tgtcgggcgg cgacggctcg gagctgcggc
     7861 aacccctggg gatcaccatt gtcggcggac tggtaatgag ccagctcctt acgctgtata
     7921 ccacgccggt ggtgtatctc tttttcgacc gtctgcggct gcgtttttcg cgtaaaccta
     7981 aacaaacggt aaccgagtaa atgacagatc ttcccgacag cacccgttgg caattgtgga
     8041 ttgtggcttt cggcttcttt atgcagtcgc tggacaccac catcgtaaac accgcccttc
     8101 cctcaatggc gcaaagcctc ggggaaagtc cgttgcatat gcacatggtc attgtctctt
     8161 atgtgctgac cgtggcggtg atgctgcccg ccagcggctg gctggcggac aaagtcggcg
     8221 tgcgcaatat tttctttacc gccatcgtgc tgtttactct cggttcactg ttttgcgcgc
     8281 tttccggcac gctgaacgaa ctgttgctgg cacgcgcgtt acagggcgtt ggcggcgcga
     8341 tgatggtgcc ggtcggcaga ttgacggtga tgaaaatcgt accgcgcgag caatatatgg
     8401 cggcgatgac ctttgtcacg ttacccggtc aggtcggtcc gctgctcggt ccggcgctcg
     8461 gcggtctgct ggtggagtac gcgtcgtggc actggatctt tttgatcaac attccggtgg
     8521 ggattatcgg tgcgatcgcc acattgatgt taatgccgaa ctataccatg cagacgcggc
     8581 gcttcgatct ctccggcttt ttattgctag cggttggcat ggcggtatta accctggcgc
     8641 tggacggcag taaaggtaca ggtttatcgc cgctgacgat tgatggcctg gtcgcagttg
     8701 gcgtggtggc actggtgctt tatttgctgc acgccagaaa taacaaccgc gctcttttca
     8761 gtctgaaact gttccgcact cgtacctttt cgctgggcct ggcggggagc tttgccggac
     8821 gtattggcag tggcatgttg ccctttatga caccggtttt cctgcaaatt ggcctcggtt
     8881 tctcgccgtt tcatgccgga ctgatgatga tcccgatggt gcttggcagc atgggaatga
     8941 agcgaattgt tgtacaggtg gtgaatcgct ttggttatcg tcgggtactg gtagcgacca
     9001 cgctgggtct gtcgctggtc accctgttgt ttatgactac cgctctgctg ggctggtact
     9061 acgttttgcc gttcgtcctg tttttacaag ggatggtcaa ctcgacgcgt ttctcctcca
     9121 tgaacaccct gacgctgaaa gatctcccgg acaatctggc gagcagcgga aacagcctgc
     9181 tgtcgatgat tatgcaattg tcgatgagta tcggcgtcac catcgccggg ctgttgctgg
     9241 gactttttgg ttcgcagcat gtcagcgtcg acagcggcac cacacaaacc gtctttatgt
     9301 acacctggct tagcatggcg tttatcatcg cccttccggc gttcatcttt gccagagtgc
     9361 cgaacgatac gcatcaaaat gtagctattt cgcggcgaaa aaggagcgcg caatgaagtt
     9421 ctggcgacct ggtattaccg gcaaactgtt tctggcgatt ttcgccacct gcattgtctt
     9481 gctgatcagt atgcactggg cggtgcgtat cagttttgag cgtggcttta ttgattacat
     9541 caagcatggt aatgaacagc gattacaact gttaagtgat gcgcttggcg agcagtatgc
     9601 gcagcatggc aactggcgct tcctgcgcaa caatgatcgc tttgtctttc agatcctgcg
     9661 ttcatttgaa cacgataatt cggaagataa acccggcccg ggtatgccac cgcacggctg
     9721 gcgtacccag ttctgggtgg ttgatcaaaa caacaaagtg ctggttggtc cgcgagcgcc
     9781 gattccacct gacggtacac ggcgacccat tctggtcaac ggtgcggaag ttggcgcggt
     9841 gatcgcctcc cccgttgagc ggttaacgcg caatactgat atcaatttcg ataaacaaca
     9901 gcggcaaacc agctggttga ttgtcgccct ggcaacgtta ctcgcggcac ttgccacttt
     9961 tctgctggcg cgcggtttac tggcaccggt aaaacgactt gtcgatggca cgcacaaact
    10021 ggcggcgggc gatttcacta cccgcgtaac gcccaccagt gaagatgaac tgggcaaact
    10081 ggcgcaagac ttcaaccagc ttgccagcac actggagaaa aaccagcaaa tgcggcgcga
    10141 ttttatggcc gatatttctc acgaactgcg tacgccatta gcggtgctgc gcggtgaact
    10201 ggaagccatt caggatggcg tgcgtaaatt cacgccggag acggtggcgt ctttacaggc
    10261 ggaggtcggt acactgacca aactggttga cgatctccat cagttgtcga tgtctgatga
    10321 aggcgctctc gcctatcaaa aagcaccggt agatttgatc ccactgctgg aagtggcggg
    10381 cggcgcattt cgcgaacgat tcgccagtcg tggcctgaaa ctgcaatttt ccctgccaga
    10441 cagtattacc gtatttggcg atcgcgaccg tttaatgcag ttattcaata acttactgga
    10501 aaacagcctg cgctacactg acagcggcgg cagcctgcaa atctctgccg ggcagcgcga
    10561 caaaacggtg cgcctgacct ttgccgacag tgcgccaggt gtcagtgacg atcagctaca
    10621 aaaattgttt gaacgttttt atcgcaccga aggttcccgc aaccgtgcca gcggcggttc
    10681 cgggctgggg ctggcgattt gcctgaacat tgttgaagca cataatggtc gcattattgc
    10741 tgcccattcg ccttttggcg gggtaagcat tacagtagag ttaccgctgg aacgggattt
    10801 acagagagaa gtatgaccga gttaccaatc gacgaaaaca caccgcgtat tttgatcgtg
    10861 gaagatgaac cgaagctggg gcagttgctc attgattatc tgcgtgctgc gagctatgcg
    10921 ccgacgctta tcagccacgg cgatcaggta ctgccgtatg tgcgccagac accaccggat
    10981 ctgatcctgt tggatctgat gctccctggc atcgatggct tgacgctgtg ccgggaaatt
    11041 cgtcgttttt ctgacattcc tatcgtgatg gtgacggcaa aaatcgaaga gatcgaccgc
    11101 ctgctcggac tggagattgg cgcagatgac tacatctgca agccgtacag cccacgggaa
    11161 gtggtagcgc gcgtcaaaac cattttgcgc cgttgcaaac cgcagcgcga gttgcagcaa
    11221 caggatgctg aaagcccgtt gattatcgac gaaggtcgtt ttcaggcttc atggcgcggt
    11281 aaaatgcttg acctgacacc tgcggaattt cgtctgctga aaacgctctc tcatgaacca
    11341 ggaaaagtgt tctcccgcga gcatttgctc aatcatcttt atgacgacta ccgcgtagta
    11401 accgaccgca ccatcgacag ccacattaaa aacctgcgcc gcaagctgga atctctcgac
    11461 gccgaacagt catttatccg cgccgtttac ggcgtcggtt accgctggga agccgacgcc
    11521 tgccgcatcg tttagtttta gcgacatgac tttgttagcc ggagatggcg ttcagaccag
    11581 atccggcaac attatcccac gcatggtcag caaactgaca ttatctctcc gccctggcag
    11641 gcatcagaaa atcgctcata ctttaatcgg taaacagcac ctttagatgc tgttttcgat
    11701 acgcaatttc aatcaaggag tcattatggc tggttggttt gaactcagta agagcagtga
    11761 taatcagttc cggtttgtgc taaaagcggg caatggtgag actatcctca ccagcgagct
    11821 gtatacctca aaagcctctg cggaaaaggg catcgcgtcg gtgcgtagca acagcccgca
    11881 agaagaacgc tatgagaaaa aaacggcaag taacggcaaa ttctatttca atctgaaagc
    11941 cgctaatcat caaattatcg gctccagcca gatgtacgcc accgcgcaat ctcgtgaaac
    12001 cggaattgcc tccgttaaag ccaatggcac aagccagacg gtgaaagaca atacgtaatt
    12061 acgatgccgg gcgtgggttg ttggcgtccg gctttatatc caaataacgc ttagttatta
    12121 ataattaaga gtaaccgccg ccattaaaat agtgggcact gacgagcact cgttgtgctt
    12181 tctttgttta ccatcccata cttatcctcc tgaacatgtt catgtgccaa aatatcttct
    12241 aatactttcg tgacgctatc cggtttatga catacactga tgtaagattt ttacttacag
    12301 gcgggcgtaa aatggccaga acgatgacgg ttgatctcgg cgatgagttg cgtgaattta
    12361 tcgagtcctt aattgaatcc ggcgactatc gcacccaaag cgaagtgatc cgtgagtcac
    12421 tgcgattact tcgagaaaag caagcagagt ctcgcctgca agcgttacgt gacatgctcg
    12481 cagaagggtt gagtagtggt gaagctcagc cgtgggaaaa ggacgcgttc ttacggaagg
    12541 tgaaagcagg gataaggaaa taatgtgcgt attattaaac tcatgccaaa agccaatgag
    12601 gatttagaag gcatctggta ttacagctat caccatttcg gcgaaccaca agccgacaga
    12661 tatgtggaac atctttcaga cgttctccag attctgagta acaataatat aggtacgccc
    12721 agacctgaat taggcgaagg tatttttgtt cttccttttg aacgccacgt catttatttc
    12781 ctgcaatcac caggggaaat tatcgtcatt cgtatcctta accaaaacca ggacgccacc
    12841 agacatttac actggagcta aacccgccga ttgcgctaca atgcccgccc ttaaagtggg
    12901 ggcactcccc taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga
    12961 attatgttta aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct
    13021 ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc gttattccct gcgtgtgcgc
    13081 aacaacgaat tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg
    13141 aaaaagtttt atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc
    13201 cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg
    13261 ctgattatgc tggtgcgtga gcacttccct gaaatgccga tccacctttc ggtgcaggct
    13321 aacgccgtga actgggcgac ggtgaaattc tggcagcaaa tgggcctgac ccgcgtgatc
    13381 ctctctcgcg agctgtcgct ggaagagatt gaagagatcc gcaatcaggt gccggatatg
    13441 gagatcgaga tcttcgttca cggcgcgctg tgcatggcct actccggtcg ctgcctgctc
    13501 tctggctata tcaacaagcg cgacccgaac cagggcacct gcaccaacgc ctgccgctgg
    13561 gagtacaacg tccaggaagg gaaagaagat gatgttggca acatcgtaca caagtacgag
    13621 ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg gcgcaccaac cgacaaagtg
    13681 tttatgatcg aagaggccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac
    13741 ggcacttaca tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc
    13801 aaaatgggcg tgcattcgct gaaaatcgaa ggtcgtacca aatctttcta ctattgtgca
    13861 cgcaccgcac aggtttaccg caaagctatc gatgacgccg ctgcgggaaa accgttcgat
    13921 accagcctgc tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg
    13981 cgtcgtcata ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc
    14041 cagcagtttg ttggtgagtt taccggtgag cgcaaggggg acctcgcggc ggtagcggtg
    14101 aaaaataaat tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaat
    14161 tttacccttg agcacatgga aaacgccaaa ggcgaagcta tgccgatagc accaggcgat
    14221 ggttatactg tgtggctccc ggtcccgcag gatcttgagc tcaattacgc gctgctgatg
    14281 cgtaatttct ccggggaaac cacgcgtaat ccccacggta agtgattaat ttcgattatt
    14341 tttcccggat ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc
    14401 atccgtttcg ctgaaaaaca taacccataa aatgctagct gtaccaggaa ccacctcctt
    14461 agcctgtgta atctccctta cacgggctta ttttttatgc ataagcccta tccctggtca
    14521 ccgtcttcca ttgaccacat cgatagaatc tcccttcata gcacgatgcc tttcacgtaa
    14581 cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag aacgcgcatg tttgatgctt
    14641 accaaaaaat attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct
    14701 gcaccttctg tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg
    14761 gctgcatcat tttttactat attttctgca ccagatattc tgcccctggc taaagaagct
    14821 tcggcttcgg tgtagtattg attatcgagt ttacgctgaa tattactttt atatgcaaga
    14881 ccaaatttac cgatacttgt ctcatcatta tgcacagcac aaccagacat aataaaaat
//
LOCUS       DAPPNJ010000114        14920 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000114 DAPPNJ010000000
VERSION     DAPPNJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.115"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..326)
                     /locus_tag="RPM73_002859"
     CDS             complement(<1..326)
                     /locus_tag="RPM73_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521201.1"
                     /translation="MTWQNVPYAFEKTTGELTLVIEKLPPIEISSSFPFETLITALAG
                     VIAAGITGWVAYRAIKENFALATLQAHLNTNKELAQQIRFAGAEHVTDVIMLASTFEQ
                     WHLVGN"
     gene            complement(433..1011)
                     /locus_tag="RPM73_002860"
     CDS             complement(433..1011)
                     /locus_tag="RPM73_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDX6521202.1"
                     /translation="MINPSEVGKSGEMVRLRTLESIWIQGKLRMWGRWSYIGGGSGGN
                     MFNQLLASGKITKTAINEALRRMKIAGITKPELEAYLREILNSKNKSGLAFCSDEEGL
                     LVDGVIASVLMNDDYRSLYSVIVDRYRLRKSKLQMANELQAKHPDWPLITCRRRIDTW
                     LSLAESILYAPVCDAFGTNSDRFKLQSEQESA"
     gene            complement(1026..2015)
                     /locus_tag="RPM73_002861"
     CDS             complement(1026..2015)
                     /locus_tag="RPM73_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDX6521203.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADAPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVQSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2023..2832)
                     /locus_tag="RPM73_002862"
     CDS             complement(2023..2832)
                     /locus_tag="RPM73_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDX6521204.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKNRPKYWLSNK
                     QTCELIEQLFTEGGITPLEQNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2852..3241)
                     /locus_tag="RPM73_002863"
     CDS             complement(2852..3241)
                     /locus_tag="RPM73_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDX6521205.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSVLASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(3238..3564)
                     /locus_tag="RPM73_002864"
     CDS             complement(3238..3564)
                     /locus_tag="RPM73_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDX6521206.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERGLKV"
     gene            complement(3564..4058)
                     /locus_tag="RPM73_002865"
     CDS             complement(3564..4058)
                     /locus_tag="RPM73_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDX6521207.1"
                     /translation="MIMSLLNEVQKYIEAHPGCTSRDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSSDEWYLSGN
                     YVGA"
     gene            complement(4055..4996)
                     /locus_tag="RPM73_002866"
     CDS             complement(4055..4996)
                     /locus_tag="RPM73_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX6521208.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(4986..5165)
                     /locus_tag="RPM73_002867"
     CDS             complement(4986..5165)
                     /locus_tag="RPM73_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDX6521209.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(5341..5892)
                     /locus_tag="RPM73_002868"
     CDS             complement(5341..5892)
                     /locus_tag="RPM73_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521210.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(5936..6136)
                     /locus_tag="RPM73_002869"
     CDS             complement(5936..6136)
                     /locus_tag="RPM73_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX6521211.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            6227..6901
                     /locus_tag="RPM73_002870"
     CDS             6227..6901
                     /locus_tag="RPM73_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HDX6521212.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            7136..7342
                     /locus_tag="RPM73_002871"
     CDS             7136..7342
                     /locus_tag="RPM73_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521213.1"
                     /translation="MHKTEPTIVAPGYTNEEIYEWMAKKLAAINQLREVLSYRQETID
                     SLKKLDQEITVLSQDVTLDIVQTN"
     gene            complement(7314..7748)
                     /locus_tag="RPM73_002872"
     CDS             complement(7314..7748)
                     /locus_tag="RPM73_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521214.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGVVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            7892..8202
                     /locus_tag="RPM73_002873"
                     /pseudo
     CDS             7892..8202
                     /locus_tag="RPM73_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8217..8579
                     /locus_tag="RPM73_002874"
     CDS             8217..8579
                     /locus_tag="RPM73_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521215.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            8645..9469
                     /locus_tag="RPM73_002875"
     CDS             8645..9469
                     /locus_tag="RPM73_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HDX6521216.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            9597..10133
                     /gene="yfdR"
                     /locus_tag="RPM73_002876"
     CDS             9597..10133
                     /gene="yfdR"
                     /locus_tag="RPM73_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDX6521217.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            10124..10486
                     /locus_tag="RPM73_002877"
     CDS             10124..10486
                     /locus_tag="RPM73_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521218.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            10486..10791
                     /locus_tag="RPM73_002878"
     CDS             10486..10791
                     /locus_tag="RPM73_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521219.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     RETYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            11018..12181
                     /locus_tag="RPM73_002879"
     CDS             11018..12181
                     /locus_tag="RPM73_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDX6521220.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            complement(12196..12271)
                     /locus_tag="RPM73_002880"
     tRNA            complement(12196..12271)
                     /locus_tag="RPM73_002880"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12236..12238),aa:Thr,seq:cgt)
     gene            complement(12386..13639)
                     /gene="proA"
                     /locus_tag="RPM73_002881"
     CDS             complement(12386..13639)
                     /gene="proA"
                     /locus_tag="RPM73_002881"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDX6521221.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(13651..14754)
                     /gene="proB"
                     /locus_tag="RPM73_002882"
     CDS             complement(13651..14754)
                     /gene="proB"
                     /locus_tag="RPM73_002882"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDX6521222.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         3816 a         3836 c         3559 g         3709 t
ORIGIN      
        1 ttatttccga ccagatgcca ttgctcaaat gtgctggcca acataattac gtctgtcaca
       61 tgctcagcgc cagcaaaacg tatttgttgc gccaattctt tattagtatt caagtgagcc
      121 tgtaatgtgg ctaaggcaaa attttctttg attgccctgt atgcaaccca accagttatt
      181 cctgcggcaa taacgcctgc aagagcagta atgagtgttt caaaaggaaa ggaagaactg
      241 atttcaattg gcggtaattt ttctattacc aaggtcaact cgccagtagt tttttcgaaa
      301 gcgtacggta cgttttgcca agtcatgaag cctcctgagt ttcagaggaa tcataacaaa
      361 aaaacccgct caatggcggg ctcttaatgt tgtgttgctc agttcgcttt aacgtcccga
      421 gcctaccaca atttaagcac tttcttgctc actctgcaac ttaaatctgt cgctatttgt
      481 gccgaatgcg tcacaaactg gagcgtacag gatcgattct gcaagactta gccaagtgtc
      541 aatgcgtcga cgacaggtga tcagcggcca gtcaggatgt ttagcctgca gttcattggc
      601 catctgcaac ttgctcttac gaagacgata ccggtcaaca atcacgctat agagcgatcg
      661 gtagtcatca ttcatcagta ctgaagcaat gacaccgtcc actaacaacc cttcttcatc
      721 tgaacagaac gccaggccgc ttttattttt gctgttgaga atttcacgca ggtacgcttc
      781 aagttcaggc ttggtgatac ccgctatctt catccggcgt agcgcttcat tgatagctgt
      841 cttggtgatt ttcccggatg ccagaagctg gttaaacatg tttccaccac taccaccgcc
      901 gatataagac cagcggcccc acatgcgcaa cttgccttgt atccagatac tttccagagt
      961 acgaaggcga accatttcac ctgatttgcc aacttcagaa ggattaatca tttagcattc
     1021 tccacttacg ccagtacgcc tattgccagc gcacgatcga taaaacgaaa tatcagctcc
     1081 agctgggagc catacttctc ttcaaatgcc acggtatccg catgcagctc gtcgtgatgc
     1141 tttctgcaca aaggcaacac aaagaggtca tgcgcttttg tacccattcc accctgaccg
     1201 tgacctatca ggtggtgggg atcatcagca ggctttccac aacatgcaca cggctgtgtc
     1261 ttaacccagc gcgtgtactt ttcattaacc cagcggcgac gtttggggcg taacataaaa
     1321 gactccggcg actccggatc cactttcagc gccagcacct ttttcgcctt atcctggatg
     1381 atgctggtgg caggaaccga aggcacaagg tcactttccc gggtgacaga ctgcacaaca
     1441 ggcttcggta atctcagtgc cttacgggct gcactttccg gtaaggcatc tgccagatca
     1501 ttacgaatca gccaccagca cagttccggc attgtcacaa cgtgactatc atcaaaaccg
     1561 agatcccgac gcacaacaga caacacccag cgggcacagt tatccgttgc cattgattcc
     1621 agccgttccg tgaactgatc gcgcagctgg ttatcgcagt gccagcacag acggattgcg
     1681 cccggagcgt gtcgcattgt ggtcatgttc tcgctgtgcc agtcggaatg aggccactgg
     1741 cagccttttt cacgaagtaa ccagctttca agacattcca cgccaccagc acgacggatc
     1801 actgcctcat tgcggaacac ggcccgaacg gcaggagcat ccgccagcgg ttgtgatgcc
     1861 gccggaacag caccactggc gaaagatgaa taacgttccg gctcaggctc cagcaggaca
     1921 cgcccctgca taaacaaggg catcagctct gaacctggcc tgaacaatac gatccccata
     1981 cgcggggcaa tttcaggggt cagtagtgct ctcacggtca cctcaatgaa cggtatcgag
     2041 cagctttaac agctcaggga atcgggattc gaagaaatgc ggctgcgtct cgcgcggatt
     2101 tgcgggactg gtgatgttct tgccgaacat gcagcctttc gctgtcagcg accagaattt
     2161 tttgatgttg ttaatcgcgg tacggctgta tcgttcgcgc tgctcgacga tccccagctt
     2221 caccatctgg tgatatgcct gattagccgt caggcggata ccatactgtt tcagcagtgc
     2281 actcagtgac agtgtcgggc gacttgagcc atcaggcgcg tcagcaggag catcaatggc
     2341 atagcgcggt gccagattcg gtaagccaac agcctcctgg agtttctgac aggccccaag
     2401 cacagatgag ttagacaggt ttaactcccg acgcataaag tccagcagaa tcacaccagc
     2461 ctgcatcttg tcagcagcct gtccggataa tttttccggt gcactggtta ccatatcgaa
     2521 agtacggatc accttcagat ggaatgacgg gctgatccac attgcatagg catacaccag
     2581 ttctttgcag acatacgtcc cctggttatt tccgccatta atgacgctaa ctggttgatt
     2641 ttgttccaga ggcgtaattc caccctcggt gaaaagttgt tcaatcaatt cacaggtttg
     2701 cttattggag agccagtatt tcgggcggtt tttttgttct cccccggctg ccctgtgcag
     2761 atcgttcagg ctgtaacgcc cataagcatc acgacgaact tcaataccat caatgaccat
     2821 cagattattc atacttcgtt tctcctctta atcaggcggc tgcacccgcc gttttctcgt
     2881 acttactgat agtgatctcg accttccctt ccgggataac cggtccccac tccaccagca
     2941 ttcttttcac ctggctgtcg tcttcccaca cacccgcgtg ggtcagggcg tcaaacagcg
     3001 ccttgttata gttgtccaga tcgcggatcc ggttatccgg aggaaacaac acgatctcca
     3061 ctgaagcaag taccgacgtt ggttttggca gacgacgtaa ctgctcaact attgctgcgc
     3121 acgccgcgct ctggaatttt cgccccgccg cgcttatcag gctcttacca gcaaacgccc
     3181 ctttgttggg gtgtcgccag tacgtgttca cgctgggcgg aaaaggcagg atcagcttca
     3241 tactttcagg cccctctcat gtaaccagtg ggttgcacgc agccttgcgt tttcctcacc
     3301 ggcaagcagt gcgcggataa tcccgaccgc ctcgctgtcg tcgtccttca ccgcggtatg
     3361 aagcgttatc ccccgggcca cgccacgctt tatcgtgatg acgccttttt tctccagtgc
     3421 gcgaagatgc tctaccgctg cattcactga acggtatccc agcatggttg ccacctcctg
     3481 attggttggc ggaaagccac gctctttctg ataagaaatc agcatatcca gcacctgctg
     3541 ctggcattga gttaacgtcg tcattacgcc cccacgtaat tccctgacag ataccactca
     3601 tcactcgata cagcgcgctt gctgcttttc cgtaaacact gctcacgacg cgccagaaaa
     3661 ttgtttcgtt ctggctggga gtggctttca cggaatgccg ccatccacac cgttgcagca
     3721 cgacggtata agcccctgga ctccagttct tcagcctggc gggtcaggca caaaatctcc
     3781 cgcgggtcgt tagtgccgac atagaaattg cgcacaggtc tggtttcacg aactggttgc
     3841 ggttctgcct cctgcgctct ctcagtcagg cgcgggaaat gtctgcgtgt atccccttca
     3901 caacggtgag ccacacgacc actctgacgt aacttgcttg ctgactgcag aacgcgctgc
     3961 cgtgagtaac ctgcaaaagc atccgcaatg tctctggaag tacaccccgg atgggcttca
     4021 atgtatttct gaacttcatt caaaagactc atgatcaccc cctgaatcct gctgggatct
     4081 ggctgtagtc cacgttgtcg taactggctt tgaagtacgg gtcttcgcgt ttttcggtgt
     4141 acgtgctgac ggacggcgat aagcgcaggg aaagctcatc ccatttttcc cgcagcttcg
     4201 acgggctgag cacgttacgg caccagaacg gatcgcggct gacgcggctg tacatctcgc
     4261 agatttgttt gtgagtacga ccatcctgca cacacatcag gcgaatttcg tttgcccagg
     4321 ctgtccagtt cggttctttg ggacgaacca cctcgccgtc acattcggcg gcatgctcgt
     4381 acagggcgat gatttttttc cagagccact gtgcgcaggt caaatcatcc tgcgttcccc
     4441 actggcgctt tttagggctg aatacaaccg catcaggatg gcgagttaaa aaatcctgtt
     4501 catccgtctg cgtgtccggt tgcgaagcgt ccggacgaga aggtttttta tctgacggat
     4561 catgttttga ttttactgac ggatccccgc cagattctga cgggtgaaaa cccgcttttt
     4621 tgccagattt cgacgcatca aattttgacg ggtcagattt tgatgcgtca gattttgacg
     4681 ggtcagaatc tgacagttga gaaaaagccg cagcctgaag cttcgcaacg ttaagctgat
     4741 aaacattcga cgcattgcgg ttaccctggc gacgcgcctt acgcgttaac cagccttctg
     4801 cttccagccg tgcgatagcc gttctgacgg tactcatccc cgcgccaatc tggcgggcaa
     4861 tggtttcaat tgatggccag cacacacctt cgtcattact gaaatcagcc aggcgtgcca
     4921 taattgccac gctggataat ttcatgcctg acgctgcgca accatcccat acatagccgg
     4981 ttaatttagt gctcatgacc gacctctatt tccctgaatt tacgacgaaa ctgttcgagc
     5041 ggactgaagc attcatgctc atagccttcg cggaggtaga taacccgttg tgtttccggc
     5101 tcccaacgaa tgactctgac gggtactccg tagtgatctt tgaaccagcg gttaacttgt
     5161 cgcaaaggac tgtctccttt tgccggttaa aatcacccac agcccactct gcaaagctgt
     5221 gggttacaat taccctgcca cctggtacat ttactgcata gcaatactcc accttcgctt
     5281 ttccacccgg tacaggaagc gcaatcagtt gcgagcgacg gtagtgtgtt gttaaactat
     5341 tcatgcgtta gtttctccac agtcacgaca cgccacggcg cccggagctg cacactcgcg
     5401 ggcgtcatta ctttctgaaa tgcaaaaaat tttgtagacc agtgctgcat gctcctgcag
     5461 cttcgaaatt gagaggtaca gctcgtcgtt aattgctgtc ttctcatgcg gttccactac
     5521 accgtcttca attgctgaac gaatctgttt tgaataactg ccgatctgtt caatgacctc
     5581 cagcaggcgt tggttgatat cggcgttgtc cacatcctcg acatcaggaa gagacacaaa
     5641 gacgccattt gcagactgcg ccacagcgtc agcaatgaag tgagttccac cagcacgttg
     5701 caaaatcatt gcccatccca gcgggaaaat ctgatcgcca tcggcacgaa ggcggttaaa
     5761 taatgcgttc tctgttacat ccagccagtc agctgcttca gcgtaaccac ccggcaactt
     5821 tgcgatagtt tttctgacag ctttcacgta ccactcaggc tgtttttcta ctttccagtg
     5881 atgcttaccc acggttagcc tcatcgttct gtggttaaaa attgaaagtg ttctgctaat
     5941 ctttcggata gatatccggt cttaagtcag atttcgtaat tgcacctgac gtgcattgct
     6001 caagtttttt agccagcaca aaactggctt ttttatagcc attgaaaacc agccgtaagt
     6061 agccaggtgt tgagccaact tttccggcca actcgccctg ctgttctttg gttaaagagt
     6121 cccaatacgc tttcatacaa tatgtacctc cagtgtacat attacatgat taaaatgaac
     6181 cttcaagata cttgtacctt aacggtacaa gggttttaat ttcgttatga aaacaatcca
     6241 tgacatccgg cggtctaacg ccagaaaact gagagatggt gttggcggga attcttcctt
     6301 tgccactatg attgatcgcg agccaaccca gaccagcagg tttatgggag atggtgctac
     6361 taaaaatatc ggtgacagca tggcacgaca catcgaaaaa tgtttcgacc tgcctgttgg
     6421 atggctcgat caagaacacc agacaacgaa catcacaaaa aaacctgatg tttcaatcac
     6481 taataaacaa atcacattag tccctgtcat atcatgggta caggccggag catggaaaga
     6541 agttggatat tctgaggttg atttgagcac agcagaaacg tatccctgcc ctgtaccctg
     6601 tggggaaatg acttatatct tgcgggtgat aggtgattca atgattgatg agtaccgccc
     6661 gggagacatg atttttgtcg atcctgaagt accagcctgc cacggtgacg acgttattgc
     6721 attgatgcac gatacaggtg aaaccacctt caaaaggttg atagaagatg ggacacagcg
     6781 ttatctcaaa gcgttaaacc caaactggcc tgagccttac attaagatca acggtaattg
     6841 ctctataatt ggaactgtga ttttctcagg aaaaccaaga agatacaaaa tcaaagccta
     6901 atcaatgttt atgaacctgc ttcggcaggt ttttttatac ttgacaatgt acccatgaga
     6961 tacataatgt acccaagaga aacaacgaac aggcaggacg cccacgaagt agccgcctgg
     7021 ggcatataaa gtctagggtg attcgttagc aacaaaaaag cgccctacag gacgcttagc
     7081 tctttaacaa tctggtcccc atcaacaagt aactgataac ttgaggaggt gtgaaatgca
     7141 caaaacagaa cccacaatcg tcgcgcccgg atacacaaat gaggaaattt acgagtggat
     7201 ggcaaagaag ctggcagcta taaaccagct tcgtgaagtg ctgtcttatc gacaggaaac
     7261 aatagactcc ttaaaaaaac tggatcagga aatcacggtt ttatcacagg atgttacttt
     7321 agatattgtg cagacaaatt aggatcccat tcattttcgt caaaatcatc aaagtgatga
     7381 atttgtgatc tccagtctcg ataatctaaa aatttctggg cggttacgct tattttatca
     7441 agtgtgagtt catcctgaat tgaaagaaga agttcatcaa atttcatctc attaatctgt
     7501 tttggcatcc agtgatgctt catcagaata aggtgaacca gagccttttt cccattcaac
     7561 tgattatagg gagtgccgaa tttcttccgg tgctcatgta agacaaggtc cagaagagta
     7621 agtaatgttg cccttgattc cactttgctt atttcgactg atgacactac cccactgatt
     7681 tcaatgcccc gatactttcc aacattttca cagtgggatt tgtacagcgt gtagatatta
     7741 ccggacattt cttttccttt tgcgttgttg gggataacca gattaaccga atccttgttg
     7801 ttggggaata accaggtcca cctcgcctga tgtggctaaa agcaggcaca taacagctaa
     7861 gtatttttaa ccagagagaa tccttagcgt tgtggtgaat gcggctcagc gcacgcgggt
     7921 taaggttgag gctgacagtc gaccttctgt ggatacccac ccgtctggtg tgcaaccttc
     7981 gccaggcacc gggaggcacc cggcaccaca actttatgct gtgtgtagtc ctggaggtac
     8041 cagtttgtac ccttgcttcc ggctggtacc gtccttttta caaaacagag aagagcatca
     8101 ccggacgacg ggctcataac ccaatccatc cgggcggctg ccaccgcagg tgttcttctc
     8161 tgttttgtgg agaaactaat cggccttgca gggtcgatat gatgaggagc agcaaaatgg
     8221 ctagcgaacg cagtactgat gtgcaggcat ttatcgggga gctggacggc ggcgtatttg
     8281 aaaccaaaat cggcgcagtt ctcagtgaag tcgcttccgg tgtgatgaac acgaaaacca
     8341 aaggtaaggt ctcactcaac ctggaaatcg aaccatttga tgagaaccgt gtgaaaatca
     8401 aacacaaact ctcatatgtt cgcccgacta accgcgggaa aatttccgaa gaagacacca
     8461 ccgaaacgcc gatgtatgtc aatcgcggtg gtcgcctgac tattctgcag gaagaccagg
     8521 gacagttact gactcttgcc ggtgaacctg acggaaaact ccgcgcagca ggtcgttaat
     8581 atcgttttta attaactgat tatttatctc atcactgaat atctttatat agtgaggact
     8641 tattatgtct cagaacttag acgcaaccgc aattaatcaa atccatgccc ttatttctgc
     8701 tcagggtgtt aatgaaatta tcagtaagat tggtgccgat gctgtggcat tgcctgagaa
     8761 tttccgcatt catgatctgg aaaaatttaa tttaaatcgc ttccgtttcc gtggtgcgct
     8821 ttccactgcc agcatcgatg actttacccg ttattctaaa gatcttgcag atgaaggcac
     8881 ccgctgcttt atcgatgctg ataatatgcg tgccgtcagt gtgcttaacc tgggtactat
     8941 tgatgaacca ggtcacgcag ataacaccgc cacactcaaa ctgaaaaaga cagcaccgtt
     9001 ctctgctctg ttgtctgtta acggcgagcg taactcccag aagtcactgg cagaatggat
     9061 tgaagactgg gccgactatc ttgtgggctt tgatgctaat ggtgacgcta ttcaggcaac
     9121 aaaagcggct gcggctgtcc gtaaaatcac gattgaagca aaccagaccg ctgattttga
     9181 agataatgac ttcagcggca aacgctccct gatggagtct gtcgaagcga agaccaaaga
     9241 cattatgcca gtggcatttg aatttaaatg cgttccgttt gaaggtctga aagaacgtcc
     9301 gtttaaatta cgcctcagta ttatcactgg cgatcgtcct gtactggttc tgcgcattat
     9361 tcagctggag gcggtgcagg aagaaatggc taacgaattt cgtgatctgc ttgttgagaa
     9421 attcaaggac agcaaagtag aaacctttat tggtactttc accgcctgat ttcattactg
     9481 caaatgccct tgcgggggca tttatggaaa cgtaatttac tcaataatcg ccggatggtg
     9541 agggattctt tttaccagaa ttcagcgcgg tgcagcgcat atacgtggag aacaaaatgt
     9601 catttattaa aactttttcc gggaagcatt tttattatga caggataaat aaagacgaca
     9661 tcgatattaa cgatatcgcg gtttcccttt caaatatctg tcgctttgcc ggtcatcttt
     9721 cgcacttcta cagcgtcgcc caacatgcgg ttctttgcag ccagttggtg ccgcaggaat
     9781 ttgcttttga agcgttaatg catgatgcaa cagaagcgta ttgccaggac attcccgcac
     9841 cactgaaacg ccttcttcct gactataaac aaatggaaga aaaaatagac gccgtaatcc
     9901 gtgagaaata cgggttaccc ccagttatga gtacgcccgt gaaatatgcc gatctcatca
     9961 tgctggcaac cgaacgccgc gatctcgggc ttgatgatgg ctctttctgg cctgtactgg
    10021 aaggtatccc ggcaacagag atgttcaacg tgattccact ggcaccgggc catgcctacg
    10081 ggatgtttat ggaacgcttt aacgagttat cggagttacg caaatgcgca tgaatgtttt
    10141 cgaaatggaa gggtttcttc gtgggagatg tgtaccgcga gatctgaaag taaatgaaac
    10201 agatgctgaa tacctggtgc gtaaattcga tgcgcttgaa gctaaatgtg cagcacagga
    10261 aaacaaagta ataccagtgt caactgaact gccaccagca aatgaaagtg ttttgttatt
    10321 cgatgctaac ggagaaggct ggctaattgg ctggcgttct ctctggtaca cctggggaca
    10381 aaaagaaacc ggagaatggc agtggacatt tcaggtcggg gaccttgaaa acgtcaatat
    10441 cactcactgg gcagtaatgc caaaagcacc ggaggctgga gcataatgac cacttttacc
    10501 gacaaagaac tgattaaaga aattaaagag cgtatcagca gccttgacgt gcgagacgat
    10561 attgagcgcc gtgcttatga aatcgcactc ctatctctgg aagtagaacc agatgaacgc
    10621 gaaacttatg aattattcat ggaaaagcgt ttcggtgact tagtagatcg tcggagagca
    10681 aaaaacggcg ataacgaata catggcatgg gatatgactc tcggttggat cgtctggcag
    10741 caacgagctg gtatccattt ttcaacaatg tcacagcaag aggtgaaata atggagccat
    10801 acagcctcac actcgatgag gcctgtcatt ttctcaagat atccagaccg actgccatta
    10861 actggatacg cacagggcgt cttcaggcaa cacgcaaaga tcccactaag aataaatctc
    10921 cttacctcac aacacgacaa gcctgcattg cggctcttca gtctccgctg catactgtcc
    10981 aggtgagcgc gggtgatggc ataacagagg aaagaaaatg tcactcttcc gcagaggtga
    11041 aatatggtac gccagtttca cattgccgaa cggtaaaaga tttaaacagt ctcttggaac
    11101 aaaggacaaa aggcaggcga cagaactcca tgacaagcta aaggctgaag catggcgggt
    11161 cagcaaactt ggtgaaatac ctgatataac gttcgaggaa gcgtgtgtca ggtggcttga
    11221 agagaaagca cataaaaaat cactggacga tgacaaaagc cggatcggat tctggcttca
    11281 acatttcgca ggaatgcaac taagagacat tactgaatca aaaatttatt cagcaatgca
    11341 gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc agggcagaag catgcagaaa
    11401 aaaagggaaa cctgttccag aatacacgcc aaaaccagcg tccgttgcaa cgaaggctac
    11461 gcatctttca tttataaagg ccctactaag agccgcagag cgtgaatgga aaatgctgga
    11521 taaggcacca attattaaag tgcctcaacc aaagaataaa cggatccgct ggctggagcc
    11581 ccatgaagca caaaggctga ttgatgaatg tccggagcca ttaaagtctg ttgttgaatt
    11641 tgcactggca acaggcttaa gacgctcgaa catcatcaac cttgaatggc aacaaataga
    11701 tatgcagcgc cgggtggcat ggataaaccc ggaagagagt aaatcaaacc gcgcaattgg
    11761 cgttgcgctg aatgatactg catgtcgcgt tttgaaaaaa caaatcggga atcatcaccg
    11821 ttgggtattt gtgtacaagg aaagctgtac caaaccagac ggaacgaaag cgccaacagt
    11881 aaggaagatg cggtatgacg caaacacagc ctggaaagcg gcgctgagac gggctggtat
    11941 tgatgatttc agatttcacg acttgagaca cacctgggca agttggctgg ttcaagccgg
    12001 agtcccgttg tcagtgttac aggaaatggg aggctgggag tctatcgaaa tggttcgtcg
    12061 atatgctcac cttgcaccta atcaccttac cgaacacgca cggcaaatag actcgatcct
    12121 gaacccatcg gtcccaaatt cgtcccagtc aaaaaataag gaaggtacta atgatgtgta
    12181 acttattgat ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa
    12241 tgcgctgctc taccaactga gctatatcgg ccctgaaagg acatgttcac gaacgtgaat
    12301 cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa tggccttgtg aatcaaatgg
    12361 ctacttttgc atcacccggt tttatttacg cacgaatggt gtaatcacca atgccgatcc
    12421 acttgtaagt ggtcagtgct tccagcccca ttgggccacg cgcgtggagt ttttgtgtgc
    12481 ttaccgccac ttccgcaccc agaccaaact ggccgccgtc ggtaaaacgc gtagaggcgt
    12541 taacgtaaac agcggacgaa tccacttcgt taacaaaacg ctgggcgttg cgcatatcgc
    12601 gggtcaggat cgcatcggag tgttgtgtgc cgtgttcacg aatatgggcg atggcatcgt
    12661 caagatcgct gacgattttg acgttcaaat ctaatgacag aaactcatcg tcatactctt
    12721 cggctttaac agcaaccacc ttcgcagggc ctgcctgcaa ctgcgccagt gcagctgcat
    12781 ctgcgtgtaa tgtcacgccg ctttccgcca tttgtttgct taatgcgggc aggaagctat
    12841 cggcgatgtt tttattcacc agcaacgttt caaccgtatt acatgtgctc ggacgctgag
    12901 ttttcgcgtt gacgatcact tttaatgctt cagcgatctc tacactttca tcaacgtaaa
    12961 tatggcatac gcctatacca cctgtgatca ccgggattgt cgactgttca cggcacagtt
    13021 tatgcaaacc agcgccacca cgcgggatca gcatgtcgat gtatttatcc atacgcagca
    13081 tttcactgac cagcgcacgg tcaggattat caatcgcctg cacggcaccc gccggtaagc
    13141 cgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc cgcgttagtg cgacacgttt
    13201 ctttgccacc gcgcaggatc accgcattac cggttttcag gcacagcgaa gcgacatcaa
    13261 ccgtcacgtt cgggcgcgct tcataaatca cgccaataac ccccagcggt acgcgacgac
    13321 gctcaagacg caggccgctg tccagtacgc tgccatcgat tacctgcccc accggatcgg
    13381 cgaggttaca cacctggcgc acatcatcgg caatgccttt cagccgtgcg ggcgtcagtg
    13441 ccagacggtc aagcatcgct tcgccaaggc cattggcacg cgcgtcagca acatcctggg
    13501 cgttagcgtt gaggatgatt tcgctttgtg cttccagttc atcggcgatt ttttccagca
    13561 cgcgattttt ttcgcggctg gagagttgcg ctaatttata cgaggcttgc ttcgcggcaa
    13621 tgcccatttg ttccagcatc agcctgctcc ttaacgggta atcatgtcat cacggtgaac
    13681 ggcaaccggg ccgtattcat atcccagtat tgcatcaatt tcttgcgagt ggtgcccggc
    13741 aatacggcgt aatgcatcgc tgttgtaacg actgacgccg tgggcgatat cgcgaccttc
    13801 gaggttgcaa atgcggatga cttcaccacg cgagaaattg ccagtcacgc ttttaatgcc
    13861 tttcggcaac agggagctgc cgcgttcaag aatggcggca gttgcccctt catctaccgt
    13921 gatttcaccc gccggcggcg caccgaaaat ccagcgttta cggttttcaa gcggagtcgc
    13981 ctgggcatgg aacagcgtac cgacggaaat gccttccatc acatcaccaa taacgcccgg
    14041 cttgctgccc gcggcaataa tggtgtcgat acccgcacgg caagccacgt cagcggcctg
    14101 caatttggta ctcatgccgc cagttccgag gcctgaaacg ctgtcaccgg caatcgcgcg
    14161 cagtgcgtca tcaatgccgt aaacatcttt aatcagttct gcctgcggat tgctgcgcgg
    14221 atcagcggta tacaaacctt tttgatcggt cagcagcaac agtttatcgg cacccgccag
    14281 aatcgccgcc agcgcagaaa ggttatcgtt atcgccgacc ttaatctctg ccgtagcgac
    14341 agcatcgttc tcattgatta ccggaacgat attgttatcg agcaacgcac gcagggtgtc
    14401 gcgggcgttc aggaagcgtt cacggtcttc catatcagca cgggtcagca gcatctgccc
    14461 gacgtgaatg ccataaatcg aaaacagctg ttcccacagt tgaatcagtc gactctgccc
    14521 taccgccgcc agcagttgtt tcgaggcgat agtcgctggc agttccgggt accccaggtg
    14581 ctcacgtccg gcggcgatcg cgcccgacgt cacaataaca atccgatgcc cggcggcatg
    14641 taactgcgcg cactggcgaa caagttcaac gatatgggca cggttcagac ggcgcgatcc
    14701 gcctgttagc acactggtgc cgagttttac caccagcgtc tggctgtcac tcatgattct
    14761 ctgccattca attttaggaa aaatgatatc aaacgaacgt tttagcagga ctgtcgtcgg
    14821 ttgccaacca tctgcaagca aagcatggcg ttttgttgcg cgggatcagc aagcctagcg
    14881 gcagttgttt acgcttttat tacagattta ataaattacc
//
LOCUS       DAPPNJ010000115        14631 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000115 DAPPNJ010000000
VERSION     DAPPNJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.116"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(170..424)
                     /locus_tag="RPM73_002883"
     CDS             complement(170..424)
                     /locus_tag="RPM73_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDX6521223.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            625..1359
                     /locus_tag="RPM73_002884"
     CDS             625..1359
                     /locus_tag="RPM73_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDX6521224.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1361..1972)
                     /gene="emtA"
                     /locus_tag="RPM73_002885"
     CDS             complement(1361..1972)
                     /gene="emtA"
                     /locus_tag="RPM73_002885"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDX6521225.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2072..2986
                     /gene="ldcA"
                     /locus_tag="RPM73_002886"
     CDS             2072..2986
                     /gene="ldcA"
                     /locus_tag="RPM73_002886"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDX6521226.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLTMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            3081..4817
                     /locus_tag="RPM73_002887"
     CDS             3081..4817
                     /locus_tag="RPM73_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDX6521227.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5203..6273)
                     /gene="dadX"
                     /locus_tag="RPM73_002888"
     CDS             complement(5203..6273)
                     /gene="dadX"
                     /locus_tag="RPM73_002888"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDX6521228.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6283..7581)
                     /gene="dadA"
                     /locus_tag="RPM73_002889"
     CDS             complement(6283..7581)
                     /gene="dadA"
                     /locus_tag="RPM73_002889"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDX6521229.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7911..9443
                     /locus_tag="RPM73_002890"
     CDS             7911..9443
                     /locus_tag="RPM73_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDX6521230.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIADSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(9495..10214)
                     /gene="fadR"
                     /locus_tag="RPM73_002891"
     CDS             complement(9495..10214)
                     /gene="fadR"
                     /locus_tag="RPM73_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDX6521231.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10436..11977
                     /gene="nhaB"
                     /locus_tag="RPM73_002892"
     CDS             10436..11977
                     /gene="nhaB"
                     /locus_tag="RPM73_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDX6521232.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12123..12653
                     /gene="dsbB"
                     /locus_tag="RPM73_002893"
     CDS             12123..12653
                     /gene="dsbB"
                     /locus_tag="RPM73_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDX6521233.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12699..13967)
                     /gene="umuC"
                     /locus_tag="RPM73_002894"
     CDS             complement(12699..13967)
                     /gene="umuC"
                     /locus_tag="RPM73_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDX6521234.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLQVK"
     gene            complement(13967..14386)
                     /locus_tag="RPM73_002895"
     CDS             complement(13967..14386)
                     /locus_tag="RPM73_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDX6521235.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         3426 a         3829 c         3660 g         3716 t
ORIGIN      
        1 caacgaaaca ataattaaaa aaggattctg ttgttgttac cgggaaccga ctttatcgga
       61 tatcgaagtg ctatattgat ttttttcacc cgtaaagcag gtgtcgagta tacgttctga
      121 tgtgaaagcg gatagcggca tctgtatcgc tatccgcctt atgaaaatct tattctcttc
      181 gcaggaggcg gaatatgccc agaacgagaa tagctcccac caccgccacc aggaagctgt
      241 gcagattaaa accactgatg gagccgccaa tgccaaacat ggtcgccagc cagccgccga
      301 ccaccgcacc gactatcccg agaatacagg tcaggaaaaa tccaccacca tcacgccccg
      361 gcatgattag cttggcgata atgccggcta tcaggccaaa aataatccag gcaataattc
      421 ccatgttcag gccctcgttt cgatgaatcg gacgcaagaa atgcttacat caacgctcta
      481 agtataggca agggcgggat cgatgctggg cgtatgtcgg ctgatttaag aacagcatgt
      541 aaaaaatatt cacacatttg tattaagttt tgttaactgt gaccgataaa ccaaagacag
      601 tttgtcagtc aggagttttt ccgcgtgagt cattaccatg agcagttcct gaaacaaaat
      661 ccgttagccg tcctgggcgt gttacgcgat ttgcacaaag ccgcaattcc tttgcggctc
      721 agttggaatg gcgggcagct gatcagcaaa atactggcaa taaccccgga taaactggtg
      781 ctggatttcg gcagtcaagc cgaagacaac atcgccgtgc taaaggcgca gcacattacc
      841 attaccgccg aaactcaggg tgcgaaagtc gagtttactg ttgaacaact acagcagagt
      901 gaatacttgc agcttccggc atttattacc gtaccgcctc ccaccttatg gtttgtacaa
      961 cgacgccgat atttccgcat ctccgcccca ctccatccgc cttatttttg ccagaccaaa
     1021 ctggcggata acagtacgtt acgtttccgc ctgtatgatt tgtcgttagg cggcatgggc
     1081 gcattactgg aaacagcaaa gcctgccgaa ttacaagaag gcatgcgctt cgctcagatt
     1141 gaagtcaaca tggggcaatg gggtgttttt cactttgacg cccagttaat ctccatcagc
     1201 gagcgcaaag tgattgatgg caagaatgaa accatcacca ctccccgtct gagcttccgt
     1261 tttcttaacg tcagcccgac ggtggagcgg caattacaac ggattatttt ctctctcgag
     1321 cgggaagccc gggagaaagc ggacaaagta cgcgactgat ttacatcgcg tccagtgcct
     1381 gctcaagttt gtagatatag cgcggagcct gcggcgcagg gtgatttcgc gctacgtgtt
     1441 cgaggaactc gtcagcatcc aaatcgttga ttttgctgat cgcctttttc cgatctgacg
     1501 agaaagtccg tagcagcgca cctgccccgt tagcgtatga caccaccagc gcatattgca
     1561 gtaccttcgg atcttcaatg cctgccagcg ggccggtttc cagaatattc aggtaagccg
     1621 cccccattga aatattacgc tctggatttt tcagttcgct ggtcgtcggc tcaccactcc
     1681 agcccatacg gcgataaaca tcacgtccgg aggttgaagc ttttaactgc atcaaaccaa
     1741 tggcattcga tttactcacc gcgttcggat taccacccga ttcgatagcg ataatcgccg
     1801 tgatcaattg tggatcgacg ccccaggctg caccggcttt ctggcttatt ggcatccact
     1861 gcatcgcacg ttgcaccgga actttcgcgt tccacggcgg gttcgtatag tcatgctttg
     1921 atgaacaacc cgctaataac acaatcaaaa aggcaaacca tctcaatttc acttcttcta
     1981 tccttatagc ctggaatcgc cgttgaggtg acaacggcac acctaagcgg catgatatac
     2041 atttcgtttc tgttgtcagc aaggaattgc catgtctctg tttcacttaa ttgccccatc
     2101 gggttactgc attaaacagc acgccgcgct gcgtggtatc cagcgcctta ccgacgaggg
     2161 gcatcaggtc aataacgtcg aggttattgc ccgtcgttgt gagcgttttg cgggtacgga
     2221 aacggagcgt ctggaagatc tcaattccct tgccagactg actaccccca acaccatcgt
     2281 gctggctgta cgcggcggtt acggtgccag tcgtttactg gcagatattg actggcaggc
     2341 actggtggct cgccaacaac atgacccgtt gctcatttgc gggcatagcg attttaccgc
     2401 cattcagtgc ggtcttctgg cgcaaggcaa tgtcatcacc tttagcggcc ccatgctggt
     2461 agcaaacttt ggcgcggatg aactgaacgc ctttaccgag caccatttct ggctggcgtt
     2521 acgcaataaa accttcacca ttgaatggca aggggaaggc ccgacatgtc agaccgaagg
     2581 cacgttgtgg ggaggcaatc ttacgatgct gatttcactg attggtacac cgtggatgcc
     2641 aaaaattgag aacggtattc tggtgctgga agatattaac gagcaccctt tccgggtcga
     2701 acgtatgctg ttgcagctct atcatgcggg aattttgcca cgtcagaagg cgatcattct
     2761 cggtagcttt agcggcagca cgcccaatga ttatgacgca ggttacaacc tggagtcagt
     2821 gtacgcgttt ttgcgttccc gcctgtcgat tccactcatt accggcctcg attttggtca
     2881 tgaacaacgc acggttacgc tgccgttggg cgcacatgcc attctgaata acacccggga
     2941 aggcactcag ttgacgatta gcggtcatcc tgttcttaaa atgtaaaaaa atcacgcttc
     3001 agggctaagt aaaacgctgt ctctgccgct aatatggtag ggtttatata agagacagcg
     3061 taatcaggag taaccgaccg ttggatgcca caacaataat tagccttttc atcttaggat
     3121 ccatccttgt caccagcagt atattactta gttcattttc ttcccgtctt ggcattccta
     3181 ttctggttat ttttctggcg atcggcatgc tggcaggagt cgatggcgtc ggcggtatcc
     3241 cgtttgataa ttaccccttc gcctacatgg taagtaacct ggcactggcg attattttgc
     3301 tcgacggcgg gatgcgcact caggccagtt cctttcgtgt ggcgttagga ccggcactgt
     3361 cgctggcgac gctgggcgtg cttatcacct ctggtttaac cggcatgatg gcggcgtggc
     3421 tgtttaatct tgatttgatt gaaggcttat taatcggcgc tatcgtcggc tctaccgatg
     3481 ctgcagcggt cttttctctg ctgggtggta aggggcttaa cgaacgtgtt ggctcgacgc
     3541 tggaaattga atccggcagt aatgatccaa tggcggtctt tctgacgatt accctgattg
     3601 cgatgatcca gcaacatgaa agcagtgtca gttggatgtt cgtggtcgat attctgcagc
     3661 aatttggcct cgggattgtc atcggacttg gcggtggtta tttactgctg caaatgatta
     3721 atcgcatcgc cctgcctgcc ggattatatc cattgctggc attaagcggc ggtattttaa
     3781 tttttgcttt aaccacggcg ctggaaggca gcggtattct ggctgtttat ctgtgcggtt
     3841 ttctgctggg taatcggccg attcgcaacc gctacggcat cctgcaaaat ttcgacggcc
     3901 tcgcctggct ggcgcaaatc gccatgttcc tggtgctggg gctattggtt aacccaagcg
     3961 atctgctgcc tattgccatt ccggcgctca ttttgtcggc atggatgata ttcttcgccc
     4021 gtcccctttc ggtatttgct ggattgctcc ccttccgcgg tttcaatctg cgtgagcgcg
     4081 tgtttatcag ctgggtagga ttacgcggcg cggtgccaat catcctggcc gtgttcccga
     4141 tgatggcggg gctggagaat gcacgtctgt tctttaatgt cgccttcttt gtggttctgg
     4201 tttcactgct cttgcaggga acatcactct cgtgggcggc taaaaaagcc aaagtggtgg
     4261 ttccgccagt gggacgtccg gtgtcacgcg ttggcctgga tattcatccg gaaaatccgt
     4321 gggagcagtt tgtttatcaa ttgagtgccg ataaatggtg cgtgggcgcg gcactgcgcg
     4381 atttgcatat gccaaaagag acgcgtattg cggcactgtt tcgtgataac cagttgcttc
     4441 atcctaccgg cagcacccga ctgcgcgaag gcgatgtgtt gtgtgtaatt ggtcgggaac
     4501 gcgatcttcc ggcgctcggt aaactgttca gccagtcgcc gccggtcgcg ctggatcaac
     4561 gcttctttgg tgacttcatt ctcgaagcca gcgccaaata tgctgatgtt gcgctgatat
     4621 atggcctgga agacgggcga gaatatcgtg ataagcagca aacgctgggt gaaattgtcc
     4681 agcagttgtt aggcgcggca ccggttgtcg gtgaccaggt agagtttgcc gggatgatct
     4741 ggacggtggc cgagaaagaa gataatgaag tgttgaagat tggcgtacgg gtggctgaag
     4801 aggaagctga atcttaaatt taatgaagca tctatcgccg gttgcgatgc tttgctgaac
     4861 gcagcctgag cctgcaattg tttgtcgtcc ataatttaac cttcatttga tgttgaattg
     4921 aacatatcaa aatcaggcaa ttacacaaat ctatgtacag gctccatcaa gggtaaagcg
     4981 tgatttatct gaagtcgact ccgagtcaac aatgaagcgt aagcgtttta tgtagaacgc
     5041 ctgtgcggtg agttcaggtt ccggggaaat agtgtccatc cttttaatgg acactatcat
     5101 gggcaatggc tctgtcacat taacaactac agttgctgac cagccggatg gatgattttt
     5161 tcgcaaccag aagacgctgc ctccgatccg gcttacaaca agttacactg tcacaaccgg
     5221 gacgcgtaac gccagcgcgc acatcaactc atagcccacc gttccggcag cggcggcgac
     5281 atcatcaatt ttgatctcct tgccccacag ctcaaccggc gtaccaatac ccgcctgcgg
     5341 gcaaggcgtt aaatcgaccg ccagcatatc catcgagacg gtccccaccg tcatggtgcg
     5401 cacgccgtcc actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta
     5461 ccctgcggcg acaatgccga ttcgctgttc atcgcgcgca gtatagcgac cgccgtagcc
     5521 cacacgctca cccgctttta gcgtctggac accaataatc tcactgctta gagtcatcac
     5581 cggacgtaat ccggtattgg cgatatcacg ccactgaccg gacggcgaag cgccatacaa
     5641 aataatgcca ggccgaaccc agtcaaaatg cgcttccggg tgccacagag tcgccgccga
     5701 attggacaac gaacgccgac actccagccc ttccgccgcc tgctcaatac gcgccatcgc
     5761 gctggaaatt ccatcaggat gttccgcttc ggcaaaatgc gacatcaggg tcatctcgcc
     5821 aacattcgcc attgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc
     5881 caaccgattc atcccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc
     5941 attttgcagt gctttgagct gccagttgct gtgtacgcag gtggtcaggc ggtgctggtc
     6001 ataaatctcc agatcctgag catggaaaaa tccttccagc atcaggatcg gccccttcca
     6061 gccgcgctca cgtaacgtta ttgcctcttc caggttaagt aatgcaaagc catcggtggc
     6121 cccgagcgcg ctccagatac gctcaatacc atgcccgtaa gcgttcgctt ttaccaccga
     6181 ccagacgcgc gcgtgcggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg
     6241 cagatcgagg ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg
     6301 taaatggccc ggacgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagatcctc
     6361 atatgggatc gctggcgtgc gaccagagag cagatcgctt aacaactgac cggaaccgca
     6421 agccatcgtc cagccgagcg tgccgtgacc ggtattcagc cacagatttt taaagcgtgt
     6481 acgcccgaca accggcgtac cgtctggcgt catcgggcgc aaacccgtcc agaaagtcgc
     6541 ctgctcgaca tgaccgccgc gtggatagag atcgcgaacc accatttcca gcgtttcacg
     6601 acgcggttgc aacagctcgg tgttaaaacc aacaatttcc gccattccgc caacacgaat
     6661 gcggttatcg aaacgggtaa tggcgatttt gtaggtttca tcaagaatag tggatactgg
     6721 cgcaccatct tcctgcgcaa ttggaatagt cagcgagtag cctttcagcg gatacaccgg
     6781 aatatcaaca atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc
     6841 atcggcctta atcacttcat cgccacactt cacgccgtag atttgctcgc cgtcgcaaag
     6901 cagttggtca acgggcgtat taaagcggaa tttaaccccc gcctgctctg ccatccgcgc
     6961 cagattctgg gtaaacagct ggcagtctcc ggtttcatca ttgggcagtt gcagaccacc
     7021 cgtcagtttg tgcgctactt ctgccagcgc gggctccact tccgccaggc ggctggattc
     7081 cagcagctga tacggtacgc cggcatcttc cagcacggcg atatcgcggg tcgcattttc
     7141 atactgttgt tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat
     7201 attggtttcg gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgcaccat
     7261 ccgccctttg ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca
     7321 tttcaactga aactgcgtac cgtcgagacg gaccgccagc ggcgcatggc gctggaacat
     7381 ccatttgatc gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga
     7441 gatttgcccg gcattcgcgg cgctggtttc cagggcggcc cccggctccc gatcaatgac
     7501 ggtgacctca tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact
     7561 tcccagtatg acaactcgca tagccactcc gataacggta gatacagtaa aagaataata
     7621 atctaattac atcttgataa ctcagttgaa aatattattc aacatacggc ttttttatgg
     7681 tgagctggct cacatctccc tgactctgcg gcaatatggc gtctttagta tctcctgctg
     7741 aacgcccttt ttatccagca taaaattccg ttcagaagcg gattagtggc actctgataa
     7801 taagaaatcg caaagaaaaa aattctccgg cgacgcggtt tttaacaggt gttctatgct
     7861 tgaaatgagg tgctccacac agagagcgcc gacaacaatg aggtgcgcgt atggcgacga
     7921 tcgattctat gaataaggac accacacgtt tgagcgatgg acccgactgg acgttcgacc
     7981 tgctggatgt ttatctggca gagatagacc gggtggcgaa actctaccgg ctggatacct
     8041 acccgcacca gattgaagtg ataacctcag aacagatgat ggatgcctac tccagcgtcg
     8101 gcatgccaat taactatccg cactggtcat tcggtaaaaa gtttatcgag actgaacggc
     8161 tgtataagca cggtcagcaa ggactggcct atgaaatcgt cattaactct aacccgtgta
     8221 tcgcttacct gatggaagag aacaccatta ccatgcaggc gctggtgatg gcacacgcct
     8281 gctatgggca taactctttc tttaaaaaca attacttatt ccgcagttgg accgacgcca
     8341 gttcgattgt cgattatctg atttttgccc gtaaatatat taccgagtgc gaagaacgtt
     8401 atggcgttga tgaagtagaa cggcttctgg actcgtgcca cgcgctgatg aactacggcg
     8461 tggaccggta caaacgcccg caaaaaatct cgctgcaaga agagaaagcc cggcagaaaa
     8521 gtcgcgaaga gtatctacaa agtcaggtca atatgctctg gcgtaccctg ccgaagcgcg
     8581 aggaagagaa aacggttgct gaagcgcgcc gctatccgtc cgaaccacaa gaaaacctgc
     8641 tctattttat ggagaaaaat gcgccactgc tggaatcatg gcagcgtgaa atcctgcgta
     8701 ttgtgcgtaa ggtgagccag tatttttatc cgcaaaaaca gactcaggtg atgaacgaag
     8761 gctgggcgac cttctggcac tataccatcc ttaaccatct gtatgatgaa gggaaagtaa
     8821 cggaacgttt tatgctggag tttttgcaca gccacaccaa tgtggtcttc cagcccccct
     8881 ataacagccc gtggtacagc ggcatcaacc cgtatgccct cgggttcgcc atgttccagg
     8941 atattaaacg gatttgtcag tcgccaacgg aagaagacaa atactggttc ccggatatcg
     9001 ccgattccga ctggctggaa acgctgcatt tcgcgatgcg tgatttcaaa gatgagagtt
     9061 ttatcagcca gtttctgtca ccgaaagtga tgcgtgattt ccgcttcttc accgtgctgg
     9121 atgatgatcg gcataattat ctggagattt ccgctattca taatgaagaa ggttatcggg
     9181 agatccgtaa ccggttatcg tcgcaatata acttaagtaa tctggagccg aatattcaga
     9241 tctggaacgt ggatttgcgc ggcgaccgtt cgctgacgct gcgttacatt ccacataatc
     9301 gcgcaccgct ggatcggggg cgcaaagaag tgctgaagca tgtgcatcgc ctgtggggat
     9361 ttgatgtgat gctcgaacag caaaacgaag acggcagcat cgagttgctg gaacgttgcc
     9421 cgccaagaat gggaaatctg taaaaacaac aaaaaacccc tcgtttgagg ggtttgctct
     9481 ttaaacggaa gggattatcg cccctgaatg gctaaatcac ccggcagatt tttctgcatc
     9541 cggtgccaaa tctcgccact ctcatgccca tagcgacgca ctgtttcgta cacctgatcg
     9601 tgcgcgcctt cactgcacaa cgccgacagt ttgtggtaga agcccagcgc cagactgcgc
     9661 gcttccggat tggcgaaata gtgacgacca atgcgcgtat acagcccttt catcccgtta
     9721 aggatcagac cgtaaatcgg gttgccggaa gcaaacgcca ggccgcggaa tatgttgtaa
     9781 tccagctcgg caaaggcatc ggcgtgatcg gccacttcat tagcggtagc cagcacttcc
     9841 tgcgctttat cgggatgctg acgaaacgcg gtgcgaataa aaatagtgga aatattggta
     9901 cgcaccgaca gcaaattatc aataagctgc ggcacacttt cgtgatccag tcgcgccagt
     9961 gtttcaagga tatttaaacc agaggtttcc cagaaattat tcaccttcgt cggcttgcca
    10021 tgttgaatgg tcaaccagcc atctcgtgcc agacgctgta acacttcacg taacgtagta
    10081 cgcgttacgc caattaattc tgaaagttca cgttctgcgg gcaaaatagt cccgggaggg
    10141 aagcggttat tccagatact ttcaataatg tactcttccg cgaaacccgc cgggctttgc
    10201 gccttaatga ccatagtgag atttccataa cacagcaaaa caaagttgga ctcatcatac
    10261 cagagggcta actacgctga tagcagacaa aaagaagaaa aaagggatca aggcttcatt
    10321 ttccgcatct ctttcccctt gactatcaag acttgcctca ctcctgcctg accgttaagc
    10381 tttgcgttta aacataaaaa catcattgat tatttgtaag gtaagggaaa ccatcatgga
    10441 gatctcctgg ggccgcgcgc tatggcgcaa ttttttgggc cagtcccccg actggtacaa
    10501 actcgccctc atcattttct taatcgtaaa cccgttaatt ttcctcatca gccctttcgt
    10561 cgcgggctgg ttgctggtag cggaatttat tttcactctg gcgatggccc tgaaatgcta
    10621 cccgctgctc cccggtggtc tgttggctat cgaagcggta ttcatcggca tgaccagcgc
    10681 ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc ttgctgttat tgatgtttat
    10741 ggtggcgggt atctatttta tgaaacagct gttgctgttc atatttaccc gtttgctgtt
    10801 aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc gtggcggcag cgttcctctc
    10861 cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc agcgttgctg tcggttttta
    10921 tggtatttat caccgcgtag cctcttcccg taccgaagat accgacctgc aagacgatag
    10981 tcatatcgac aagcattaca aagtggttct ggaacagttc cgtggctttc tgcgtagcct
    11041 gatgatgcat gccggtgtcg gcactgcatt aggcggcgtg atgaccatgg tgggcgaacc
    11101 acagaacctg atcatcgcta aagcggctgg ctggcatttt ggcgatttct tcctgcgcat
    11161 gtcgccggtg accgttccgg ttctgatttg tggcctgtta acctgcctgc tggtagagaa
    11221 gctgcgttgg tttggctacg gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca
    11281 gtttgacgat caaagccgcc accagcgtac ccgtcaggat aaaatccgtc tgattgtcca
    11341 ggcgattatt ggcgtctggc tggtgactgc gctggcgttg catctggcgg aagttggctt
    11401 gattggtttg tcagtcatta ttctggcgac atcattgacc ggtgtcaccg atgaacatgc
    11461 tatcggtaaa gccttcaccg aatctctgcc attcaccgca ctgttgacgg tgtttttctc
    11521 ggtagtcgcg gtgattatcg accaacaact gttttcgcca attattcagt ttgtgttgca
    11581 ggcatcggaa catgctcagc tgtcgctgtt ctatattttc aacggtctgc tgtcatccat
    11641 ttcggataac gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg caatggaaag
    11701 tggcgctatc acgttgaagc aatacgagct gctggcggtc gccattaata ccggtaccaa
    11761 tctgccctcc gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc
    11821 actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg tggatggccc tgccttacac
    11881 cctcgtactg acactcgtcg gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga
    11941 atggtttatg caaatgggct ggatagcaac gctttgataa caacttaccg ggcatataaa
    12001 tgcccggttt gccttttcgc ccgataattg tccaattgct tcctattttt gctgcctcct
    12061 ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta tgcatattgc agggaaatga
    12121 ttatgttgcg atttttgaac caatgttcac aaggccgggg cgcgtggctg ttgatggcgt
    12181 ttactgctct ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac
    12241 cttgcgtgct ctgtatttat gaacgttgtg cgttattcgg cgttctgggt gctgcgctga
    12301 ttggcgcgat cgccccgaaa actccgctgc gttatgtagc gatggttatc tggttgtata
    12361 gtgcgttccg cggtgtgcag ttaacttacg agcacaccat gcttcagctc tatccttcgc
    12421 cgtttgccac ctgtgatttt atggttcgtt tcccggaatg gctgccgctg gataaatggg
    12481 tgccgcaagt gtttgtcgcc tctggcgatt gtgccgagcg tcagtgggat tttttaggta
    12541 tggaaatgcc gcagtggctg ctcggtattt ttatcgctta cctgattgtc gcggtgctgg
    12601 tggtgatttc ccagccgttt aaagcgaaaa aacgtgacct gttcggtcgc taatcccttc
    12661 agcgctcctg cgggagcgct tttttcctgc cgctatattt atttgacctg cagtaaatca
    12721 gaacttcgcg ttgtataacg tggtgaaagc atggcacgct tcatctgcca ttgttgctgg
    12781 atcccctgcc cggcaaaata gagtgttcct ctgccctctt tagcattcag tgtatccatt
    12841 accgtcatca attgctcact cccggggcgc ggtgcgttgt catcgaataa attgagctgc
    12901 gcgactccct gactgaagaa atcccccagc atcacgcccg ctttttggta gcgatggccc
    12961 gcttgccaga tggcatccag agatcgcgta gcagcgttaa tgatatccct gctgtcctga
    13021 gtgggcgtca gcagttttac cgacgcgcta ttgccgtaat aaggttcatt gagcgcaaat
    13081 ggtgacgtct taataaatgt ggagataaac cgacaatatt gatgctcgct gcgaagtttt
    13141 tccgccgccc gggcagcgta actacaaatg gcctgccgca tcgatggata atccgtgatg
    13201 cgttcaccaa acgagcggga acagataatt tcctgcttcg tcggtgcaaa ctcttccagt
    13261 tgcaaacagg gttcgccgcg cagttcacgc accgttcttt cgagcacgac attaaaatgt
    13321 ttacggataa accggatatc tgtatccgcc aaatcgagaa cggttttgat ccccatcgcg
    13381 tccagttttt tgctgatccg ccgtccaatc ccccagacgt catccacggg gagagcagac
    13441 attaatttac gctggcgttc cagatttgat aaatccacca ccccacccgt ctgccgctgc
    13501 catttttttg ccgcatgatt ggcaagctta gccaacgttt tggtctgggc gatccccaca
    13561 ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc aaaatcagtc
    13621 agatcgcgac aattacgcac acctgtcaga tcgcagaatg cctcatcaat actgtaaatc
    13681 tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat gtctgcgtaa
    13741 agctcataat tgctgctaaa gcaaaccacg ccacagcgac gaaacagatc tttttgtttg
    13801 aaccagggat cgcccatttt aacacccagc gcctttgcct cagcgtttcg ggcgataacg
    13861 caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg gcgaaacacc
    13921 gtctcacagc tggcataaaa cgcgtttaca tcacagagag caaacatcag cgcatcgcct
    13981 taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg atggtaatgg
    14041 gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc aattttttca
    14101 ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg gtaatagcgc
    14161 tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa tcaccacttg
    14221 ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga tcgatgcgct
    14281 gttcaacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg ctaaatagcg
    14341 gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata atctgcctga
    14401 agttatactg tttttatata cagtagtcta ttcttgccag cagatcaata ctgattcagg
    14461 ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc tgtgctcacg
    14521 tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg gcaaaaaaat
    14581 agaatagaag cattcgccat aatgacatta aacattgttt gatatttatc a
//
LOCUS       DAPPNJ010000116        14429 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000116 DAPPNJ010000000
VERSION     DAPPNJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.117"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..457)
                     /gene="hscC"
                     /locus_tag="RPM73_002896"
     CDS             complement(<1..457)
                     /gene="hscC"
                     /locus_tag="RPM73_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDX6521236.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPT"
     gene            complement(541..1476)
                     /gene="rihA"
                     /locus_tag="RPM73_002897"
     CDS             complement(541..1476)
                     /gene="rihA"
                     /locus_tag="RPM73_002897"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDX6521237.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(1594..2319)
                     /gene="gltL"
                     /locus_tag="RPM73_002898"
     CDS             complement(1594..2319)
                     /gene="gltL"
                     /locus_tag="RPM73_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDX6521238.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(2319..2993)
                     /gene="gltK"
                     /locus_tag="RPM73_002899"
     CDS             complement(2319..2993)
                     /gene="gltK"
                     /locus_tag="RPM73_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDX6521239.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(2993..3733)
                     /gene="gltJ"
                     /locus_tag="RPM73_002900"
     CDS             complement(2993..3733)
                     /gene="gltJ"
                     /locus_tag="RPM73_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDX6521240.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(3903..4811)
                     /gene="gltI"
                     /locus_tag="RPM73_002901"
     CDS             complement(3903..4811)
                     /gene="gltI"
                     /locus_tag="RPM73_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDX6521241.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            5266..7143
                     /locus_tag="RPM73_002902"
     CDS             5266..7143
                     /locus_tag="RPM73_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDX6521242.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQKIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(7221..8759)
                     /gene="lnt"
                     /locus_tag="RPM73_002903"
     CDS             complement(7221..8759)
                     /gene="lnt"
                     /locus_tag="RPM73_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDX6521243.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(8784..9662)
                     /gene="corC"
                     /locus_tag="RPM73_002904"
     CDS             complement(8784..9662)
                     /gene="corC"
                     /locus_tag="RPM73_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDX6521244.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(9752..10219)
                     /gene="ybeY"
                     /locus_tag="RPM73_002905"
     CDS             complement(9752..10219)
                     /gene="ybeY"
                     /locus_tag="RPM73_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDX6521245.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(10216..11256)
                     /locus_tag="RPM73_002906"
     CDS             complement(10216..11256)
                     /locus_tag="RPM73_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDX6521246.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(11409..12833)
                     /gene="miaB"
                     /locus_tag="RPM73_002907"
     CDS             complement(11409..12833)
                     /gene="miaB"
                     /locus_tag="RPM73_002907"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDX6521247.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            12979..14154
                     /gene="ubiF"
                     /locus_tag="RPM73_002908"
     CDS             12979..14154
                     /gene="ubiF"
                     /locus_tag="RPM73_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDX6521248.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(14308..14382)
                     /locus_tag="RPM73_002909"
     tRNA            complement(14308..14382)
                     /locus_tag="RPM73_002909"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14348..14350),aa:Gln,seq:ctg)
BASE COUNT         3477 a         3657 c         3770 g         3525 t
ORIGIN      
        1 ctgtgggttc attaattaag cgtaccgcat ttaacccggc taactccgct gctaaacggg
       61 tatgcttgcg ttgttcatcg ctgaaataag ccggaacgga gatcaccaca tctttaatcg
      121 gacgttgcag aaattcttcg gcatcttctt ttaatgagcg taataccaaa gaggacagtt
      181 ctggcgcgtt aaatgtgtcg ctgcctaacc gccagttggt attactgccc attgcacgtt
      241 taaataacgc tgccgtttta tccggatgcg aagtacgccg tgatacagcc ggttttccga
      301 ctaaaatatg attattttca tccatgctaa ttatggatgg tgttaaatat tcaccgaact
      361 tatttggaat taattgcgcg gcaccgtctt tccagacggc aattaaacta ttggtagtac
      421 cgagatcgat accaatggcg agttctgcat tatccattgc atttatatct tgaaagaaaa
      481 agagataaac agattaagac cacccaggta acgcgtggtg atcttaatca atgacgtgtg
      541 ttaagcgtaa aatttcagac gatcagccag taaatcaaca aagccctgac gatcaacatc
      601 gaccattacg gtggcattcg gtttattgcc agtcagataa taataatcaa caaccgtcat
      661 accctgagta tatttcccct gtgtttccac gccaacccag cgctcaacag aggtaaacaa
      721 ctcgggtttc aacagccagg cgatggtgca tgggtcatgc agcggtgcac cgacaaagcc
      781 ccatttttcg tctttatgat attcgaggaa gaaatccagc agttcggcaa caatggttga
      841 aacagggttg ccaatcgcgc ggaaacgctc ggtgtcttca acgtggattt gtgctttgtg
      901 agtaacatcc agaccggcca tcaccactgg gatccctgac tggaagacaa tttctgccgc
      961 ttccgggtca acgtaaatat taaattcagc cgcaggcgtc cagttaccaa gccccattgc
     1021 gccgcccata atcacgatac gggcaatttt gctatgcagt tccggatggc tattgagcag
     1081 caaggcaacg ttagtttgcg gtccggtaga cacaatcgtg acaggttccg cactttcgcg
     1141 cagcgttttc gccatcagct ctaccgccgt acagttttgc ggtgcgaacg tcggttccgg
     1201 taatgccggg ccatccagac cgctttcgcc gtgcacattg tccgcgataa tcaactcacg
     1261 cattaacggt tttaccgcgc cgcctgctac cggaatatcg gtgcgattaa gcaaggtcag
     1321 catacgaaga acattgcgta aggttttttc tggtgtctgg tttccggcgg aagacgtaat
     1381 tgctttgaca tcaagctctg gtgaggcgag ggcgagaact attgcgatag cgtcgtcatg
     1441 acctgggtcg caatctaaca gaattggcag tgccattgtt gctccttgtt gtgtgcttct
     1501 ttgcgacaag ggtaacgcca ggatgtaaca gatacgaggg gcgaaacgat aaagcgtgag
     1561 atggcgcgca cttgggtatg cgcgccagag tgattaatgc aggattttcg cgaggaagtc
     1621 tttcgcgcgg tccgatttcg gatcatcgaa gaaagcgtct ttcggcgagt cttcgacaat
     1681 tttaccctcg tccataaaga tcacccgatt cgccacttta cgggcaaaac ccatttcgtg
     1741 ggtcaccacc atcatggtca ttccttcgtt tgccagttcc accatcacgt ccagcacttc
     1801 gttgatcatc tccggatcca gcgccgatgt cggttcgtcg aacagcatcg caataggatc
     1861 catacacaac gcgcgagcga ttgccacacg ctgctgctga ccgccggaaa gctgcgccgg
     1921 aaatttattg gcatgtgcgg aaagtcccac gcgctccagc aatttcaatg ctttttcacg
     1981 agcgggcgct ttatcgcgtt taagcacttt cacctgcgcc agggtcaggt tttcgataat
     2041 cgacagatga gggaacagct cgaaatgctg gaataccatc ccgacgcggg aacgcagctt
     2101 tgccagatcg gttttcttgt cattaaccac gataccatcg acggtgattt caccttgctg
     2161 cactggttcg aggccgttga cggttttaat cagcgttgat ttgccggaac cagacgggcc
     2221 gcaaaccacc accacttcgc cttttttcac ttcggttgag cagtcggtca gcacctgaaa
     2281 gtgaccatac cattttgaaa catttttcag ggtaatcatt atgctgtcct tcttttcaag
     2341 tagctgacca acaacgacgc gctaagacta ataacgaaat aaacaaatcc ggcaaacagg
     2401 atcatctcaa cctgcgtacc atcacgctca ccaatggttg aggcggtacg gaagaaatcg
     2461 gccagagata acacatacac cagtgaggta tcctggaaca gtacgatgcc ctgagtgagc
     2521 agaagcggca ccatcgcgcg gaacgcctgc ggcagaataa tcagtttcat cgactgccag
     2581 tgagtcattc ccaacgccag cgcggcgctc gactgaccac gagaaatact ttgaatacca
     2641 gcacggataa tctctgaata gtaggccgct tcaaacatcg aaaacgccac catcgccgaa
     2701 attaaacgga tatcattttt tggtgataat cccagcacgt tttgcagaaa acctggcacg
     2761 atcaggtaaa accacagcaa aaccataact aaaggaatcg agcggaatac gttaacgtag
     2821 gctttggcaa accacgctac gggcgcaaaa ctggacaagc gcatcaccgc cagcatggtg
     2881 ccccacaaaa taccaatcac gactgccgtg acggtgattt tcagggtgat caccagcccg
     2941 tcgagcagat atggcaggga agggacaatg gaactccagt caaactcgta cattatttgc
     3001 cccccatgtt gccaggcagg cgaactttac gttcaaccag cgtcattacc agcatgataa
     3061 ccgcgttaat caacacatac gccagcgtaa tagcggtaaa cgactcccag gcgtgggctg
     3121 agtaatcaag caatttaccc gcctgcgccg ccatatccac cagaccaata gtcgaggcga
     3181 tggcggagtt tttcaccagg ttcatcatct cggaagtcat cggcgggacg ataacgcgat
     3241 aagcattagg cagcagtacg tatcgataag cctgcggtag cgtcaggccc atcgccagcg
     3301 cggcattttt ttgcccacgc ggcaacgact gaatggcggc gcgtacctgt tcgcaaacac
     3361 gggcggcggt aaacagcccc aggcagagca tggatgaaag gaaaaactga atattgggat
     3421 ccagctcggc cttaaaccac atgccgattt tctccggcaa cagctccggg atcaccagat
     3481 accaggtaaa gaattgcaca atcaacggca cgttgcggaa cagttcgaca tacaacgtac
     3541 ccagaccgga gagaaaacgg tttggaacgg tacgtaaaat gccgaaaaat gaaccgacga
     3601 ggaaagcgat aatccaggcg cagatcgaca aagcgatcgt cacctgaaaa ccactccaga
     3661 tccaaccaag ataggtggtg ttgccgaacg gggcttgttg taaaaaaata ccccagttcc
     3721 agtctataga cataaatcta ctccagaaaa aagagggtag cagcgttaac tgctaccctc
     3781 gaagattgtt acccagcgta ttgcggtttt caggcccgat ggggaacgac catcaggcgt
     3841 atagtctgtc cgtgctacgt aacaatcgag agggctggaa tttccgcccc tggttcttgt
     3901 aattagttca gtgctttgtc attcggttct ttgaacagtg ctttcatttc gtctgacagt
     3961 tcgaaattca tgttcaggtt tttcggcgga attggatttt tgaaccattt atcaaaccat
     4021 ttttccgctt caccggaagt ctgcacctga gcgatagtgt catccatcag ctttttgaac
     4081 tgcggatcat ctttacgcaa catgcaaccg taggcttcct gagactgcgg cttgccgacg
     4141 atatcccagt tgtctggttt cttcgctttc gcacgttcac cggccagcag agcgtcatcc
     4201 atcataaagg caacggcacg accgctttcc agggtgcgga aagagtcacc gtgatctttg
     4261 gcgctgatga tgcgcatatt cattttttgc tcttcattca gtttgttgag caaaacttca
     4321 gaggtagtgc cggaagtgac gactacggct ttgcctttca ggtcggcaaa atctttgata
     4381 tcgccgccct ttttggtcag caggcgcgta ccgaccacga aaatagtgtc agagaaagcc
     4441 gcctgttttt ggcgttcgac gttgttggtg gtagaaccac attcaaaatc aaaagtgccg
     4501 ttttgcagca gcggaatacg gttttgtgag gtaatcggaa tcagttttac ctgcaagtcc
     4561 ggtttgttga gtttcttttt cactgcttca acaatggcgt tggagtaatc ctgcgagtaa
     4621 cccaccactt tttgctgatt gtcgtaataa gagaaaggta ctgaagattc acgatgaccg
     4681 acgacaatca caccgttttt ggcgattttg tccagcgtac tgcccgctac cggggcggcg
     4741 tcatctgcct gtgccagtcc tgcggaaagc gccagggcga ggattgctgt ggcaggttta
     4801 cgtaattgca tatccaactc ctttatcttc tgcgttaaga acgcatggat acccgttgtg
     4861 agtgtttgtg ttgttatcgt ctgcaacttt attgtgcagt gttgtgcctg ttaggtaaca
     4921 tttagtttgg ctaaatgtaa agatattgct gttttattgt ttgtttttgc gagatgcgcc
     4981 gcaccattcc gaagcaaaat tcttaaaccg cacctttttg gtgctaccgc tgatttgtgg
     5041 tggtgcaacc tggttgcgtc gcggctgatt tcatcgctta cacagtaaat aaagcaactt
     5101 acatgccaaa aaatacttag gggcgaatta attacgattg taattttagg ctgaaaaagt
     5161 catatttcgg acagaattat cttttcggca tgggacgttt gccgggatga taactgcaca
     5221 cgggaaaaat gctttttacc tgtcttttca aggaagaaac accttatgtc tgcatcttcg
     5281 gttaagccgt taaatgttca attacccgca ataaccctta tcctttttgc actctgtatt
     5341 gggatatttt gttatctcgc acaatggatg agttatgaag aggtcgatca atccgcactt
     5401 atccatctcg gcgcaaacgt tgcgcctctt actttgtcgg gtgaaccctg gcgcttattg
     5461 agcagtatct ttctgcacag tagtgtttct catctgctga tgaatatgtt tgcattcctg
     5521 gtcgtgggag gcgtggcgga acagattctg gggaaatggc gactcctgat tacctggtta
     5581 ttctccggcg tctttggtgg gctcatcagc gcctgttatg cgttacgcga aagtgagcag
     5641 atagtcatca gcgttggggc atccggggcg attctgggaa tagctggtgc agcgatagca
     5701 acacagtttg cttcaggtgc gggcacatac cataaaaacc agcggcgagt attttctctg
     5761 ttgggtatgg tggcgctgac actgttgtat ggtgcccggc aaacaggaat agataatgct
     5821 tgtcacattg gcggcctgat tgcgggtggc gcgttgggtt ggctgagcgc gcgtttatct
     5881 gggcaaaacc gactcgttac ggaaggcggg attattgttg cgggcagtct tcttctgacc
     5941 ggggctatct ggcttgcgca gcagcagatg gatgagtcag ttttacaagt caggcaaagc
     6001 ctgcgtgaag cgttttatcc acagaagatt gaacaagagc gacggcaaaa aaagcagcag
     6061 ttagcggagg aacgcaacgc cctcagggaa acattatccg ctccggtaag tcgtgaacag
     6121 gccagtggtg atttgctcgc tgagattgcc gatatccatg atatggcgat cagtcgggat
     6181 ggtaatatgt tgtatgccgc aattgaaaac acgaacagca ttgttgtttt cgacctcgga
     6241 caaaagaaaa tcctgcatac ctttacagcg cccatagcga aagaaaagtc agtcaaacat
     6301 tgtggtggct gtaaagatca gggcgtcaga tcgctggcat taagcccgga tgaaaagttg
     6361 atttatgcga cttcatttga agcgaatgcg ttatcggtca ttaacgtggc gacaggggag
     6421 attattcagt cgattaccac cggtgcacat cctgacagtc ttatcctctc gcgtgatggc
     6481 acaaaagcct gggtgatgaa tcgcaccagt aatagtgtgt cagccattga tctggtggct
     6541 tatcagcatg tggcggatat cccgctggag aaatacgacg ggacggggac gagcggtaaa
     6601 ccaggcgcct gggttatggc actttccccg gatgaaagaa cattactggt tccgggagaa
     6661 ggcagaggta acatcgtgcg gatcaatacc atcacgcatc agaaagaaga ctttcccgca
     6721 ggtgatgcgc gtggaacgat atcggcgatg cgttttcgac ctgaaaacgg cgaggttatt
     6781 tttgccgata gtcaggggat ttcacgtata agcatagggg atcaacaaac cagcattatg
     6841 acgcaatggt gtagcaggag cgtttattcc gttgagggta ttagcccgga cggtcaatat
     6901 ttagcgttgg tgtcatatgg actgcaaggt tatgtcatcc tgctcaatat taatgccggg
     6961 cagattattg gcgtttatcc tgccagctac gttaatcacc ttcgtttttc agcggatggt
     7021 agaaaaatat ttgttatggc gaagaacggg ttgatccaac tggacaggac gcgctcgctt
     7081 gatccgcagg caattattcg tcatccccaa tatggcaatg tggcttgtat ccctgaaccg
     7141 taagaaaaag gggcatgata accatgcccc aaagctcaat gagaaagtac gttttgctca
     7201 tgcaggatac atcaggcaca tcaacgttta cgtgctcgca gactcatcag cacagcggca
     7261 aaaccaaaca gtgccgtcag cacccacagc ggccagttgc cggttcgtgc gtacggtgtg
     7321 agtccggtgg tcggcgtcac gttagtggtc aacacctcgc gggtgaactg cgggatcatc
     7381 gcctgaatct caccctgcgg gccaatcacc gccgtaatgc cgttgttggt gctgcgcaac
     7441 agtgggcgcg ccagctccag cgcacgcatt cgcgccatct ggaagtgttg ccatggacca
     7501 atagatttac caaaccacgc atcgttggag atagtcagca gatagtcggt atccgggcgg
     7561 aagttatcgc gcacttgctc gccaagaatg atctcgtagc aaatggccgc agtaagctca
     7621 ataccattta ccgacagcgg cggctggata tatggcccac ggctgaacga cgacatcggc
     7681 agatcaaaga acggtgctaa cggacgcaga atcgactcca gcgggacaaa ctcgccaaac
     7741 ggcaccagat ggtttttgtt atagcgatcg gctgattcgt agctgtacgg tgcaccttta
     7801 cccagtgtga tgatggtgtt gtaggtatcg tagcggttct gcttattgag acgcgcgtcg
     7861 acaatcccgg ttaccagcga gctaccttta tcgcgcaact caccgtccag tgctttgagg
     7921 aacggttgct gattaatttc cagatcggtt atcgccgact ccggccagat aatcaacgat
     7981 gatttgccca tcagcggagc cgttgcgttg tagtaaatct tcagcgtatt aagaagctga
     8041 tctccatccc atttcagtga ttgcggaata tcgccctgaa ccatcgaaac ctgaatggtt
     8101 ttctccggtt gtggggtaaa ccattggatg taacgcagcg ggaagggtag ggcaaacagc
     8161 acgacggcca ccaccagcgg acgccagttg cgtttgacca acgccagtgc cagcaggcca
     8221 ctaaccatca tcagcaggaa gttaatggct tccacgccca ttatcggtgc cagccctttt
     8281 aacgggccat caatctggct atagccgaac tgtaaccacg ggaagcctgt cagtacccaa
     8341 ccgcgcagaa actcggtcac ttgccagaga gaaggggcgg caatcgccac gcgcagccag
     8401 gtggttttcg gccacagacg cgacagcacg ccagcaaaca gtccggtata cagcgacaaa
     8461 tacgccgcca gcagcaccac caggaagatg ttaaccgggc caggcatccc gccaaaggtc
     8521 gcgatgctga catagaccca gttaataccg ctgccaaaga ggccaaatcc ccagcaaaag
     8581 ccaatagcgg cagactggag tggacggcgg ttaaaggtca acgcctgaag ccccatcagc
     8641 gaaataatcg ccgcaggcca gacatcgtaa ggagagaagg ccagcgttcc gcaggcaccg
     8701 aataataacg ccagcagcag gcgaatgcgc tggcgttcaa ttaatgaggc aaaagccatg
     8761 tagttatcta tccagtttcg gttttattca tccagcttcg gctggggtga gtcatccggg
     8821 attttgacat gaacctgaat aatacgccga ctgtcggcca tcgccacttt gaactggtaa
     8881 ccgtcgatgt cgatagtttc gccacgcgcc ggaagatgcc caaatgcctg catcaccaga
     8941 ccaccgatag tgtcaacctc ttcatcgcta aagtgggtgc cgaacgcttc gttgaagtct
     9001 tcaatggaag ccagtgcgcg cacggtccag gtatgacgac tcagctgacg gaagtcgata
     9061 tcatcttctt cgtcatactc gtcttcaatc tcaccaacaa ttagttccag gatgtcttca
     9121 atggtcacca gaccggaaac cccaccgaat tcgtcaataa cgatcgccat gtggtaacgc
     9181 tgagagcgaa actctttcag catccggtct acgcgcttac tttcaggaac gacaaccgcc
     9241 tgacgtaaca ctttgtccat gctgaaggct tcagcatcgc tgcgcataaa cggcagcaag
     9301 tctttcgcca tcagaatccc ttcaatgtga tctttgtctt cgctaatcac cgggaaacgt
     9361 gagtgggcgg actcgatgat gacatcaagg cattcgtcca gcgtctggtt gcgtttcagg
     9421 gtaatcatct gggagcgggg gatcatgatg tcgcgaacgc gttggtctgc gatgtccatc
     9481 accccttcga gcatatcgcg cgtatcttcg tcgataaggt cgttctgccc ggaatcacgg
     9541 atcagcgcca gcagttcgtc acggtttttc ggttcaccgt ggaaaagttg gctgagtaac
     9601 agggagaaaa atcccttctt gttgcttatc gtgtcactac tgtgtgaatt gtcgtcgctc
     9661 atggcgtcgt atgggttctc atgttagtta atcaaaacgc cgtcgttaat caccaacggc
     9721 ggggacgtct gccagtcaaa tgcctggcaa attattcttt ctcggcaatg tacggatcct
     9781 catagcccag agcaagcata atctctgttt cgagggcttc catttcttct gcttcgtcat
     9841 cttcgatgtg atcgtaacct aacaaatgca gactgccgtg caccaccata tgcgcccagt
     9901 gcgcctccag tggtttgcct tgctcctgag cttccttctc aaccacctga cggcagataa
     9961 ccagatcgcc cagtagcgac atttccatgc caggcggcac ttcaaacggg aaggagagca
    10021 cgttggtcgg cttatcctta ccgcgatagg tcagattcag actgtggctt tcggcggtat
    10081 cgaccacgcg aatagtcact tccgattctt cctgaaactg cgggatcacc gcattcagcc
    10141 atgtttgaaa ctggctctct tccggtaacc cggaattatc ttcacatgcc agttgtaaat
    10201 cgaggatcac ctgactcatt tttgttcctg ttcttcgcgc ttgcgttctg ccgccagcgc
    10261 cgcttttcgt ttttgttcgg cttcttccca ggcttcatag gcgttaacga tacgcgccac
    10321 cacagggtga cgaaccacgt cttcgctgtg gaagaagtta aagctgatct cttcgacatc
    10381 ggccagcact tcgatggcgt gacgtaagcc tgatttagta ttacgcggca ggtcgatctg
    10441 tgtgacgtcg ccggtgataa ccgcttttga gttaaaaccg atacgggtca ggaacatctt
    10501 catctgttcg atggtggtgt tctggctctc atcgagaatg ataaacgcgt cgttcagcgt
    10561 acgaccacgc atataggcca gcggcgcgac ttcaataacg ttgcgctcaa tcagtttctc
    10621 aactttctca aagcccagca tttcaaacag cgcgtcgtac agtgggcgca gatacgggtc
    10681 tactttctgg cttaaatcgc caggcaggaa gcccagtttc tcaccggctt cgaccgccgg
    10741 acgagtcagc agaatacggc gaatttcctg acgctccagg gcatcaactg ctgcagcgac
    10801 tgccaggtag gttttacccg tacccgccgg gccaacgccg aaggtaatgt catggtcgag
    10861 aatattggcg atgtactgcg cctggtttgg cgtgcgcggc ttaattacgc cgcgtttggt
    10921 tttgatattg accgctttgc cgtactccgg cacgctctcc gcgctttgct ccagtaccct
    10981 tgcttcttta atcgcaaggt ggatctgttc cggttcgata tcctgaatct gaccgcgcat
    11041 cggggcagta tcgacataca ggctacgcag aatgtctgcc gcagcggtga cgcaaatcgg
    11101 acggccggtc agtttaaagt ggttatcgcg gcgattgatc tcgatgccga gacggcgttc
    11161 gagctgcttg atgttgtcat caaacgggcc gcacaggctc aacagacgcg cgttgtctgc
    11221 tggctccagg gtgatttcgc gagtgtctat gttcaaaccg ttcctcttat ctgtatgccg
    11281 ccggaagctg aacattcacc ggcctataag gaaattattc acgccacagg aaaaaggcgc
    11341 aagcattgcg ataaagatgg ggataaagag agaaaaaaca aggcccaccg gaacggcggg
    11401 cctgagaatt acggctgata ataacccacg ccaaggtcgt tttctttgcg ggtacgggca
    11461 atcaccgatt ccggtgtttc tgccacgcgc agacccattt catcttcagt acgcaccact
    11521 ttaccgcgca gagagttcgg gtagacgtcg gtaatttcta catcgacgaa tttaccgatc
    11581 atatccggtg tgccctcgaa gttgaccacg cggttatttt cggtacgccc ggaaagctcc
    11641 atgatgctct tacgcgaagt cccttctacc agaatacgct gggtggtgcc gagcatccgg
    11701 cggctccatg ccatcgcttg ctgattgata cgttcttgca gaatatacag acgttgcttc
    11761 ttctcttctt ccggaacatc atcaaccata tcggcggctg gtgtacccgg acgtgcagag
    11821 aagataaagc tgtagctcat gtcgaaattg acgtcggcaa tcagcttcat cgttttctcg
    11881 aagtcttcgg tggtttcgcc agggaagcca acaatgaagt cagaactgat ctgaatatcc
    11941 ggacgcgccg cacgcagttt acggatgatc gctttgtact ccagtgccgt atgagtacgg
    12001 cccatcaggt tcagaatgcg atcggaaccg ctctgtaccg gcagatgcag gaagctcacc
    12061 agctccggcg tgtcgcgata cacttcgatg atatcgtcgg tgaattcgat cggatggctg
    12121 gtggtaaagc gaatacgatc gatcccgtcg atcgcagcaa ccagacgcaa cagatcggca
    12181 aacgatccgg tggtgccgtc gtagttctca ccacgccagg cattcacatt ctgaccgagc
    12241 aggttgactt cacgcacgcc ctgagccgca agctgggcga tctcaaacag aatatcgtcg
    12301 gacggacggc ttacctcttc accacgggtg taaggcacca cgcagtaggt gcaatattta
    12361 ttgcagcctt ccatgatgga gacaaacgcg gtcggccctt cggcgcgcgg ttccggcaga
    12421 cggtcaaact tctcgatttc cgggaagctg atatctacaa ccgggctgcg gtcgccgcgc
    12481 acggagttga tcatctccgg cagacggtgc agcgtttgcg gcccaaaaat aatatcgaca
    12541 tagtgggcgc gctggcgaat gtgctcgcct tcttgcgatg ccacgcagcc accgacgccg
    12601 ataatcaggt ctggattctt ctcttttaac agtttccagc gacccaactg atggaagact
    12661 ttttcctgag ccttctcgcg gattgagcag gtgttcagca gcagcacatc cgcttcttcc
    12721 gccacgtcgg tcagttgata gccgtgggtg gcatccagca gatcggccat cttcgatgaa
    12781 tcgtactcgt tcatctgaca gccccaggtt ttaatatgga gttttttggt catcgacttg
    12841 ctcttgcgaa atagtagcca ggaatgcagg gcgcatagtg taatgctttg ctgccgttgt
    12901 gaccagtatg agcgttatca gcccttgggg gtaaaaatcc tgtaaactta aagcatattg
    12961 ctaacaggat gattgaccat gacaaatcaa ccaacggaaa ttgccattgt cggcggagga
    13021 atggtcggcg gcgcactggc gctggggctg gcacagcacg gattttcggt aacggtgatc
    13081 gagcacgcag aacctgcacc gtttgtcgct gatagccaac cggacgtgcg gatctcggcg
    13141 atcagcgccg catcggtatc attgcttaaa gggttagggg tctgggatgc agtacaggct
    13201 atgcgttgcc atccttaccg cagactggaa acgtgggagt gggaaacggc gcatgtgatg
    13261 tttgacgccg ctgaacttaa gctgccgctg cttggctata tggtggaaaa caccgtcctg
    13321 caacaggcat tgtggcaggc gctggaagcg catccgaaag taacgttacg tgtgccaggc
    13381 tcgctgattg cattacatcg agataatgat cttcaggagc tggaactgaa gggcggtgaa
    13441 gtgattcgcg cgaagctggt gattggtgct gacggcgcaa attcgcaggt gcggcagatg
    13501 gcgggaattg gcgttcatgc ctggcagtat gcgcagtcgt gtatgttgat tagcgtacag
    13561 tgtgagaacg atcccggcga cagcacctgg cagcaattta ccccggacgg accgcgtgcg
    13621 tttctgccgt tgtttgataa ctgggcatcg ctggtgtggt atgactctcc ggtgcgtatt
    13681 cgccagttgc agaatatgaa tatggcgcaa ctccaggcgg aaatcgcgaa gcatttcccg
    13741 tcgcgtctgg gttacgttac accgcttgcc gctggtgcgt ttccgctgac gcgccgacat
    13801 gcgttgcagt atgtgcagcc ggggcttgcg ctggtgggcg atgccgcgca taccatccat
    13861 ccgctggcgg ggcagggggt gaatcttggt tatcgtgatg tcgatgccct gattgatgtt
    13921 ctggtgaatg cccgcagcta cggtgaagcg tgggccagtt atcctgtcct caagcgttac
    13981 cagatgcggc gcatggcgga taacttcatt atgcaaagtg gtatggatct gttttatgcc
    14041 ggattcagta ataatctgcc gccgctgcgt tttgtgcgta atcttggttt gatggcggcg
    14101 gagcgtgctg gcgtgttgaa acgtcaggcg ctgaaatatg cgttagggtt gtagccttac
    14161 aacattgccg gatgcgtgcc aaccgtaggt cggataagac gcggcagcgt cgcatccgac
    14221 attgaaggat aagacgtgtc aacatcgcat tcgacattga atgaacgcag aaaagcaaaa
    14281 agctcgccga agcgagcttt tttaatgtgg ctggggtacg aggattcgaa cctcggaatg
    14341 ccggaatcag aatccggtgc cttaccgctt ggcgataccc caacaaattg gtttttgaat
    14401 ttgccgaacg tattcgacac attcagaat
//
LOCUS       DAPPNJ010000117        14362 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000117 DAPPNJ010000000
VERSION     DAPPNJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.118"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            16..582
                     /locus_tag="RPM73_002910"
     CDS             16..582
                     /locus_tag="RPM73_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6521249.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /locus_tag="RPM73_002911"
     CDS             633..3092
                     /locus_tag="RPM73_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6521250.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKIIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPVISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEKGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /locus_tag="RPM73_002912"
     CDS             3107..3838
                     /locus_tag="RPM73_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX6521251.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPTALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3835..4896
                     /locus_tag="RPM73_002913"
     CDS             3835..4896
                     /locus_tag="RPM73_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6521252.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /locus_tag="RPM73_002914"
     CDS             5048..6094
                     /locus_tag="RPM73_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDX6521253.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="RPM73_002915"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="RPM73_002915"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDX6521254.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="RPM73_002916"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="RPM73_002916"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDX6521255.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="RPM73_002917"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="RPM73_002917"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDX6521256.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="RPM73_002918"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="RPM73_002918"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDX6521257.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="RPM73_002919"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="RPM73_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDX6521258.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLVADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="RPM73_002920"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="RPM73_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDX6521259.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="RPM73_002921"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="RPM73_002921"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDX6521260.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGDYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /locus_tag="RPM73_002922"
     CDS             complement(13199..13708)
                     /locus_tag="RPM73_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDX6521261.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13859..14362)
                     /locus_tag="RPM73_002923"
                     /pseudo
     CDS             complement(13859..14362)
                     /locus_tag="RPM73_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3465 a         3840 c         3493 g         3564 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaatcat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
     1201 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
     1321 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacagccg gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccggt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggagt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgagaaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggtgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccgactgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac tgcccaaaac
     4441 ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg gtatggcgtg
     5881 ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca ccagtcccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa
     6121 cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc
     6181 atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa tgtagagcgt
     6961 agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac tgtttgcgcc
     7021 gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctctagc
     7081 gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac caatgctgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcagctgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aatcgcgaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc tgcatgaaat
     7561 ccacaagcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta attgttcgaa
     7681 ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatag gctgcccgtt
     7861 atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg ggctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcccctgtca gagcaaacca cccatcagtt tcgaactcaa ccgttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca ggttagcaat
     8461 gcgcagcgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaac ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgaccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa
    10861 aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc cgggttctgc
    11341 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    11401 gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    11461 caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg
    11521 ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctagcg ctggagtcat aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag cttcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga
    12001 ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca
    12061 ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    12181 tgcatccagc cagtgctgtt aagctgacgc atagcggcat caacaatcgg gtatcccgtt
    12241 ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc
    12301 caggcaataa atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttcag ccaacaagcg atgcaagcac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggccgaca aacggctggt gccttccact gccggaaaat ctcgttgttg ctcataatct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccgga
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct
    12661 atcgatccgc tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc
    12781 atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata taacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtaatc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg
    13381 tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc
    13441 gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg
    13681 tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg
    13741 tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa
    13801 agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt
    13861 caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt
    13921 ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt
    13981 tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga
    14041 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    14101 catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttca gggatagctg
    14161 tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg tgcattgttg
    14221 agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa gagtgccgga
    14281 gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa tcacgcatcc
    14341 agttaataaa gcactcgtga aa
//
LOCUS       DAPPNJ010000118        14360 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000118 DAPPNJ010000000
VERSION     DAPPNJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.119"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(16..513)
                     /locus_tag="RPM73_002924"
     CDS             complement(16..513)
                     /locus_tag="RPM73_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDX6521262.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            771..1376
                     /locus_tag="RPM73_002925"
     CDS             771..1376
                     /locus_tag="RPM73_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDX6521263.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1416..2279
                     /locus_tag="RPM73_002926"
     CDS             1416..2279
                     /locus_tag="RPM73_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDX6521264.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2269..3816
                     /gene="fdrA"
                     /locus_tag="RPM73_002927"
     CDS             2269..3816
                     /gene="fdrA"
                     /locus_tag="RPM73_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDX6521265.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3816..5234
                     /locus_tag="RPM73_002928"
     CDS             3816..5234
                     /locus_tag="RPM73_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDX6521266.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5377..6327
                     /locus_tag="RPM73_002929"
     CDS             5377..6327
                     /locus_tag="RPM73_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDX6521267.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            6337..7719
                     /locus_tag="RPM73_002930"
     CDS             6337..7719
                     /locus_tag="RPM73_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDX6521268.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            8018..8428
                     /locus_tag="RPM73_002931"
     CDS             8018..8428
                     /locus_tag="RPM73_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDX6521269.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            8682..9665
                     /locus_tag="RPM73_002932"
     CDS             8682..9665
                     /locus_tag="RPM73_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6521270.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            9714..11198
                     /locus_tag="RPM73_002933"
     CDS             9714..11198
                     /locus_tag="RPM73_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX6521271.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGWKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVEGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            11191..12162
                     /locus_tag="RPM73_002934"
     CDS             11191..12162
                     /locus_tag="RPM73_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521272.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            12159..13115
                     /locus_tag="RPM73_002935"
     CDS             12159..13115
                     /locus_tag="RPM73_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521273.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            13202..14251
                     /gene="yahK"
                     /locus_tag="RPM73_002936"
     CDS             13202..14251
                     /gene="yahK"
                     /locus_tag="RPM73_002936"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDX6521274.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         3499 a         3444 c         3964 g         3453 t
ORIGIN      
        1 atttttgaat aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc
       61 gccatattct gaaattatcc ccacgcagtt acccgccaga aaacccagta acgtcaccgt
      121 ccagttcatc ccgcaggcaa aaaacagcgt catgccgaga aagccaccgg gaatgaatga
      181 cagtaaccag aaacgtcctt gccagaccac taccgctgag aaaggcacgg tagtaatcac
      241 gctcgcccag aacccagaaa tcccactcac cgaggccagc caaccggcag tcagcgcggc
      301 gaaaaacgcc cacaccatcc cggaataatt aaccgccaga cttctgataa accccgattt
      361 accgccgcct gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc
      421 ccatccttga gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc
      481 gacggtgaca cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac
      541 gaaagctact cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta
      601 tcgggtcaaa gaaacaaata ttgcactacc gcacactgcg aaaagattgt tgaacatcat
      661 tcaacaaaaa ggcgaaatac agcgaattaa atttaagcgg gttcttttat cagattatcg
      721 ttgctattct caattcatcg aaaacagcca caaaaaccag gggaatgtga atgagtataa
      781 aaaatctacc tgccgactat ttattggctg cacaacaggg tgatatcgat aaagtaaaaa
      841 cctgccttgc gctgggtgtc gatattaata cctgcgatcg tcaggggaaa acggcaatta
      901 cgctggcaag tttatatcag caatatgctt gcgttcaggc attaattgat gccggagcgg
      961 atattaataa gcaagatcat acctgtttaa atcctttttt aattagctgt ctgaacgatg
     1021 atttaacgct actacgaatt attttaccgg ctaaacccga tcttaattgc gtaacccgtt
     1081 ttggcggtgt cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc
     1141 ttttggcgca tacggagatt aacgttaacc agaccaacca tgtaggctgg acgccgctac
     1201 tggaagcgat tgtgcttaat gatggtggta ttaaacagca ggcgattgtg cagttattgc
     1261 tggaacacgg tgccagcccg catctgaccg ataaatatgg caaaacgcca ctggaactgg
     1321 cgcgggaacg gggctttgaa gagattgcgc agttactgat tgccgcaggt gcataaaccg
     1381 ggaggcttgc tatcaacaca ccagaaagac ggtgtgtgtg ggcgctaact gcggatgcgg
     1441 attttctggc gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata
     1501 tcgcactccc ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc
     1561 caaacagttg tcggttggca ctcactcact ttgatgatct gttccggcat ggtgataagg
     1621 ttcagtttga cgatcaaggt attacggttg gtcaacatct tcatatagag atgagtcgtt
     1681 gtcggcgttg gctgtcccca accttgcaaa tgaccgctgt gaattttcac cttatcgcct
     1741 ggctacagtg gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt
     1801 atcgcggcga taatccgttt tatcaggcgt taaataaaga actacatatt aaacgacggg
     1861 cagttattca ggcagtaaac gaaaaacaaa atatcgccgc agcggtcgcc agtatgattg
     1921 ggttagggat tggccttacg ccatcagccg acgattattt aacaggtctg gcgcttattt
     1981 tatttattcc cgggcatccg tcggaaaaat acaaagagga attttatctc ggtatgcagc
     2041 gcggcagaaa taacaccaca ttattaagtg ctataacgct ggaagccgca ttacaacaac
     2101 gttgccggga aaatattcat cgttttattc acaacattat ttatgacatc cctgggaacg
     2161 caactcaggc aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt
     2221 atggcatggc cgatggttgt gcgctgagcc aaacctacgg agggaattat gtcagttaaa
     2281 atagtcatta aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt
     2341 gcaaataaac tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaacaaa
     2401 ggtgtactga aaaatttagg actgctgacg ccggagctgg aacaggcgaa aaacggcgac
     2461 ctgatgattg tcatcaacgg caaagcgggc gcggacaacg aacagttgct ggtggagatt
     2521 gaagaactgt tcaacaccaa agcgcaaagc ggctcgcatg aggcgcgtta cgccactatt
     2581 gccagcgcca aaaagcatat cccggaaagt aacctggcgg tgatttcggt caacggtctg
     2641 tttgccgctc gcgaagcgcg tcaggcgctg caaaacgatc tcaacgtgat gctgttttcc
     2701 gataacgtct cagttgaaga tgaattggcg ctcaagcaac tggcccacga aaaagggctg
     2761 ctgatgatgg ggccagactg tggcacggcg attattaacg gcgcggcgct ctgttttggt
     2821 aacgccgtgc gtcgcggcaa catcggtatt gttggcgcat ccggcaccgg cagccaggag
     2881 ctgagcgtcc gcattcatga atttggcggc ggcgtttcgc aactgattgg caccggcggg
     2941 cgcgacctga gcgagaaaat cggcggcctg atgatgctcg acgccatcgg catgctggaa
     3001 aacgatccgc aaactgaaat cattgtgctt atctccaaac cgcccgcgcc tgcggtggcc
     3061 cgcaaagtgc tggaacgcgc gcgcgcctgc cgcaagccgg tggtggtctg tttcctcggt
     3121 cgtggcgaaa cgccagtgga tgagcagggg ctacagtttg cccgcggcag caaagaggcg
     3181 gcattgaaag cggtgatgct ctccggcgtg aaacaggaac atctcgacct gcatacgctg
     3241 aaccagccgt tgattgctga cgtacgtgct cgcctgcaac cgcagcagaa gtacattcgt
     3301 ggcctgttct gtggcggcac gctgtgtgac gaaaccatgt tcgcggtgat ggaaaaacac
     3361 ggcgatgtct acagcaacat tcagcccgat ccggaattcc gcctgcaaga tatcaaccgt
     3421 agcatcaaac acaccttcct cgactttggc gatgacgact tcaccaacgg caagccgcat
     3481 ccaatgattg accccaccaa ccgcatcagt cgcttgatcg aagaggcgcg cgatccagaa
     3541 gtggcggtga tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggc
     3601 tccaccatcg aggcgatcaa agaagcgaaa gcgatcgccg ccgccgaagg acgcgagttg
     3661 atcattctcg cctatgtgct gggcaccgat ctcgatacgc catcattaga acaacaaagc
     3721 cagatgctgc ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg
     3781 gcgcgtgaat ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac
     3841 cgttgaacgt tattaacgtc ggtatcgcca tgtttagcga tgacctgaaa aagcagcatg
     3901 tagaagtgac tcaactcgac tggacgccgc cggggcaggg caatatgcag gtggtgcagg
     3961 cgctggataa cattgccgat tcgccgctgg cagacaaaat cgccgccgct aaccagcagg
     4021 cgctggagcg cattatccag tcgcatccgg tgctgattgg ttttgatcag gcgattaacg
     4081 tggtgccggg catgacgccg aaaaccattc ttcacgccgg gccgccgatc acctgggaaa
     4141 aaatgtgcgg cgcgatgaaa ggcgcggtca ccggggcgtt ggtgttcgaa gggctggcga
     4201 aagatctcga tgaggcgact gaactggcgg cctccgggga gatcaccttc tcgccgtgcc
     4261 acgaacacga ctgcgtggga tcgatggcgg gcgttacctc ggcctcgatg tttatgcaca
     4321 tcgtgaaaaa caaaacctac ggcaacatcg cttataccaa tatgagcgag cagatggcga
     4381 agattttgcg tatgggtgcc aacgatcaga gcgtgatcga ccgcctcaac tggatgcgcg
     4441 atgtgcaggg gccaatgctg cgcgacgcga tgaaaattat cggtgagatc gatctgcgct
     4501 taatgctggc gcaggcgctg catatgggcg atgagtgcca taaccgcaat aacgccggga
     4561 cgacgctgct gattcaggca ctgacgccgg ggatcattca ggcaggctat tccgtcgagc
     4621 aacagcgcga agtgttcgag tttgtcgcca gcagcgacta cttctccggc ccgacgtgga
     4681 tggcgatgtg taaagcggcg atggatgcgg cgcacggcat cgaatacagc accgtggtca
     4741 ccaccatggc gcgtaacggc gtcgagttcg gcctgcgggt cagcggcctg ccggggcagt
     4801 ggtttaccgg cccagcgcag caggtcatcg gcccgatgtt tgctggttat aagccggaag
     4861 attcggggct ggatatcggc gacagcgcca tcacagaaac ctacggtatt ggcgggtttg
     4921 ccatggcgac cgcgcctgca atcgtcgcac tggtgggcgg cacggtggaa gaggctatcg
     4981 atttctcccg tcagatgcgc gaaatcaccc ttggcgaaaa ccccaacgtc accattccgc
     5041 tgctcggttt tatgggcgtg ccgtcggcaa tcgacatcac ccgcgtgggt agcagcggca
     5101 ttctgccggt gatcaacacc gccatcgccc ataaagatgc gggcgtcggc atgattggcg
     5161 cgggcattgt gcatccacca ttcgcctgct tcgagaaagc cattctcggc tggtgcgaac
     5221 gttacggcgt ctgacttaca tgtttatgcc ggatgcggtg tgaacgcctt atccggccta
     5281 cggatggccc gagaattgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt
     5341 cataacctaa tcaccctctg tagcggacat aacaacatga aagagcttgt ggtcgttgcc
     5401 attggtggca acagcattat caaagataac gccagccagt cgattgagca tcaggcggag
     5461 gcggtgaaag ccgtggccga tacggtgctg gaaatgctgg cttccgatta cgacattgtg
     5521 ctgacccacg gtaacgggcc gcaggtcggg ctggatttac gccgtgcgga gattgcccac
     5581 gagcgcgaag ggctgccctt aacgccgctg gcgaactgtg tggcagatac gcagggcggc
     5641 atcggctatc tgatccaaca ggcgctgaat aaccggctgg cgcgtcacgg cgagaaaaaa
     5701 gccgtcaccg tggtaactca ggtggaagtg gataaaaacg atccaggttt tgcccatccc
     5761 accaagccca ttggtgcatt cttcagtgaa agccagcgtg acaaactaca aaaggcaaac
     5821 cctgactggt gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg
     5881 gaaccgaaac gtattgtcga agcacccgcc attaaggcgc tgatccaaca aggttttgtc
     5941 gtaattggcg cgggcggcgg tggaattccg gtagtgcgta ctgaagcggg ggattaccaa
     6001 agcgtggacg cggttattga caaagatctc tctaccgcgc tgctggcccg tgaaattcac
     6061 gccgacattc tggtgatcac caccggcgtc gaaaaagtct gtatccactt tggtaaaccg
     6121 cagcagcagg cgctggatcg ggtggatatt gccaccatga ctcgctatat gcaggaaggg
     6181 cattttccgc ccggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggagcag
     6241 ggcggcaaag aagtgattat caccacgccg gaatgcctgc ctgcggcgct gcgcggtgaa
     6301 acgggcaccc atattattaa aacgtaagga cgtaggatga aagaaagcaa tagccgccgt
     6361 gaatttctga gccagagcgg taagatggtc accgccgccg cgctgtttgg tacctctgtg
     6421 ccgctcgccc atgcggcggt agctggcacc ctaaactgcg aagcgaacaa caccatgaaa
     6481 atcactgacc cgcattacta tctcgataac gtgctgctgg aaaccggttt tgactacgaa
     6541 aacggtgtgg cggtgcagac ccgcacggcg cgccagaccg tggagattca ggacggcaaa
     6601 attgtcgccc tgcgcgagaa caagcagcat ccggacgcca cgctgccgca ctatgacgct
     6661 ggcggtaagt tgatgctacc caccactcgc gacatgcata ttcatctcga caaaaccttc
     6721 tacggcgggc cgtggcgctc gctcaatcgt ccggcaggca ccactatcca ggacatgatc
     6781 aaactcgagc agaaaatgct gccggagtta caaccgtata cgcaggagcg ggcggaaaaa
     6841 ctgatcgatc tattgcagtc gaaaggcacc accattgccc gcagccactg caatatcgaa
     6901 ccggtttccg gcctaaaaaa tctgcaaaat ttgcaggcgg tgctggcgcg gcgtcaggcg
     6961 ggcttcgagt gtgaaatcgt cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa
     7021 cccttaatgc gcgaagcgat gcaggcgggt gcgcattacg tcggcgggct ggacccgacc
     7081 agtgttgatg gcgcgatgga aaaatccctc gacaccatgt ttcagattgc gctggactac
     7141 gacaaaggtg tcgatattca cctgcacgaa accactccgt cgggcgtggc agccatcaat
     7201 tatatggttg aaacggtaga gaaaacgccg caactgaaag gtaagttgac catcagccac
     7261 gcctttgcgc tggctacgct caacgaacaa caggtcgatg cgctggcgaa tcggatggcg
     7321 gcgcagcaaa tttctatcgc ctcgacggtg ccgattggca cgctgcatat gccgctcaaa
     7381 cagttgcacg acaaaggcgt aaaagtgatg actggcactg acagcgttat cgaccactgg
     7441 tcgccttatg gtctgggcga catgctggaa aaagccaatc tctacgcgca gctctatatt
     7501 cgtcctaacg aacagaacct ctcccgttcg ctgtttctag ccactggcga tgtattgccg
     7561 ctgaatgaaa aaggcgagcg tgtatggcca aaagcgcagg atgatgccag ctttgtgctg
     7621 gtggacgcct cctgttccgc cgaggcggtg gcgcgtatct cgccgagaac cgcaacgttc
     7681 cataaagggc aactggtgtg ggggagtgtg gcaggttgat gcgcatttga aacgcctgca
     7741 acggtgaatt gtaagagatt taagccccag ggagtggggc gggaatggtt tgctaatgaa
     7801 tgatcctgaa ctttataatc tcgcggcata atttccaggg gattatttga gagaaataca
     7861 caaaagtgag ttttaacaaa ggtatttatc aggccataaa tgtgacctgc ttcggataaa
     7921 aaagattcag tatcgaaaaa ccaatgatac tcttgctgat ggttaccaac agtaattaag
     7981 atgtgatttg tttttccgta atcgcaaaaa tattgccatg accataaaag aaaaattctc
     8041 acaaaaatat ccccatgctt ccttttgtac ttttggcgat tcagcagcgt tggccgatca
     8101 cctcgcaacg ttgattgcga ctggtgttaa aacagcttcc tgtggttcgc tggcgggatg
     8161 tattgaagac aacgcatttc cgatgattgg tgagtataag attgtagaaa atagccgtgg
     8221 tgagcctgta tgtgttatca gagtgattgg cctgcattta ctgcgctttt ctgatgtcac
     8281 cgtggagctt gcccgtaagg aaggtgaagg tgacctcagt cttgaatatt ggcgtaatga
     8341 acaccgtcgt tttttccagg cagaaggaag ctattcacca gaaatggacg ttatatttga
     8401 agaatatgcc cttattgacg ttgtgtagat aactatttat ctacgcgaaa taaactcaca
     8461 cacataaaat atcgctaatt ttgtttattt tgctattttt tacgtgatga atgtcacact
     8521 tattgttgct gcccgttttg tccatattta taaccatatt tttaatagct gcctgaaata
     8581 gggctgttta ttctgcataa aaatattcga aaattcttat tctctccagc gcgctaattt
     8641 gggcgaacac ttcctgacta ccctgcaatg aggctgaaat gatgaataaa cgttttgtta
     8701 tcaatatggt gtcttcgctg ttattaggcg ctgcgctaat ttctgcgccg ctccaggcag
     8761 cggaaaaagt cgtggtgaat atttctaaag tcgatggtat gccgtggttt aatcgcatgg
     8821 gcgaaggtgt ggttgaggcg ggtaaagcct ttggtgttaa tgcctcacag gtggggcctt
     8881 caagtactga tgctccgcaa caagttaaaa ttattgagga tttaatcgcc cgtaaggtta
     8941 atgcaattac tatcgtgcca aacgatgcca acgtgctgga accggtattc aaaaaagcgc
     9001 gagatgccgg aattgtggtt ctgaccaacg aatcccccgg tcagccaagt gctaactggg
     9061 atattgaaat cattgataac gaaaaatttg ccgctgaata tgttgagcat atggcgaaac
     9121 gcatgggggg caagggcggc tatgtgattt acgtgggtag tttgaccgtg ccgcagcata
     9181 acttatgggc tgacttgctg gtgaaatacc agaaagagca ttacccggat atgcatgaag
     9241 ttacccgccg catgccggtt gccgagagtg tggatgactc acgtcgtacc acactggacc
     9301 tgatgaaaac ctatcctgac ttgaaagcgg ttgtctcctt tggttcaaac ggtccgattg
     9361 gcgcgggacg tgcggtgaaa gagaaacggg cgaaaaataa agtcgcagtc tatggcatga
     9421 tgatcccttc acaggcggca tcactgatta aaagtggtga tatcaccgaa gggattactt
     9481 acgatccggc ctctgcaggt tacgcactcg cagccgtcgc cagcacgttg ctgaagggtg
     9541 aagagatcaa acctggatta gaaatgcaga atctgggtaa ggccgacgta gatatggata
     9601 agcgaattat ccgtttccat aaggtattac tggtcaacaa agacaatatc gactcacttt
     9661 attaatccat ttcttcgggg cgaaagcccc gattcttatt gccaggggat gttatggaaa
     9721 ctttcctttc ccttcgtcat atcaacaaaa cgtttcatgc cacgcgagcg ctgcgcgatg
     9781 tttctctcga ctttatgtcg ggtgaagtgc actgccttgc cgggcaaaac ggctgtggta
     9841 aatcgacgtt gattaaaatt atgtccggtg tttatcgccc ggatgaggga gcggaaatta
     9901 cgcttggttg gaaaaactgg tcaaagctga cacccgccgc ttcggtggcg caggggattc
     9961 aggtgattta tcaggacctc tctttatttc ctaacctgag tgtctgggag aatatcgccg
    10021 tgaatcacta tcaccacggt ctgtttgtta accgccgtcg tctgcgtgag gtggcgcagg
    10081 cggcaatgac cagtattaac gtcacattgc cactggatac actcgtttct gagctttcaa
    10141 ttgcccgctg tcagctggtg gctatttgcc gtgcactggc gcaggacgca cggttgatcg
    10201 tgatggatga accaaccgct tcgctgaccc atcaggaagt ggaagggtta ttacaggtgg
    10261 tgcatcaatt gcgtgaacgc gggatctgcg tggtcttcgt cagtcatcgt ctggaagaag
    10321 tgatggaagt ttccgaccgt atttcagtgc tgaaagatgg tgagctggtc gggacttttc
    10381 cggctgcaga gatgaccaca aaacaactcg gtttcctgat gaccggccag gagtttgaat
    10441 atcaggtgcg agagttgtgg cagggaaaat ccagcacccc ggtgctggag gtgcgtaatt
    10501 taagtcgtca tggggaatat ctaaatatta acttgcgggt ggaagcgggc gaagtggtat
    10561 cgattgttgg cctgcttggt gcggggcgca cggaattatg tctgagtctg tttggtatga
    10621 cccgaccgga tgctggcgag atcctcatca acggccagcc ggttacactg catagtaacc
    10681 aggacgctat ccgccacggt attggttatg tttcggaaga tcgcatgtcg cgcggtttgg
    10741 tgatggcgca atctattgaa gacaacatca ttagcactgt ttttcacaag gtaaaagatc
    10801 gcttcggctt tctgagtgaa actaaagttc gtgacctggt ggacaggcta atcaaggcat
    10861 tgaccatcaa agtctctgat cctcatcttc cggtgaatac gctttctggt ggtaacgcgc
    10921 agcgagtatc gatcgccaaa tggctggcga ttgggccacg actgttgatt ctcgatagcc
    10981 cgacagtcgg tgttgatatc gccaacaaag caggaatata cggcattatc agcgatctgg
    11041 cggctcatgg tattgcggta ttgatgatct gcgatgaaat tgaagaagcg tggtatcaaa
    11101 gccaccggat tttggtaatg caaaaaggtc agatcaccca tagcttcctg cctgacagca
    11161 gttctcaggc caggattgcg gaggtggtaa atggctgaac tgaaaaaacg ccatgaattc
    11221 tggctggcat tactgattgt ggtgctgttt gttggccttg cctggcgcag cgacgagttt
    11281 ctgacattcg gtaatttgta cgatctcgcc aataactatg ccatgttgac tattctcgcc
    11341 tgtggtttgt ttgtggtgtt gatttccggt ggaattgata tttcgtttcc agcaatgacc
    11401 atcattgcgc aatacggcat ggtgctgttg ctgcaaaaaa ttggtggcaa cttcgctgtc
    11461 gcgtttgcac tggcgggctg catcggcatt ttacttggct taattaacgc cttactggtt
    11521 aatcgcctac gggtgccttc tatcatcatc actatctcga cgctgaatat tttctatggc
    11581 ctgctgttat ggttgagtaa aggtgtgtgg ctgtacgact ttccgccgtg gtttgagcag
    11641 ggggttatgt tgttcaagta caccgatgct gatggctatg actatggcct tggtctgccg
    11701 ctgatcgcca tgattacggt ggtgctgcta acagcgttta tcatgaattt caccagtgta
    11761 gggcgcaaaa tttatgccct tggcgggaac cgcgaatcag ccagtcgcat cggctttagc
    11821 gtgctgaaac tgcaactttt cgtctatggc tatatgggat tgatgtctgg cgctgcgggt
    11881 gtagtgcagt cgtggacggt gatgactgtc gcccccgatt ctcttctggg ttatgagctg
    11941 acagtactgg ctgcggtggt gcttggcggc actagtttgc tcggcgggcg cggcacgtta
    12001 accggtactt tgctcggcgt ggtgttgttg gcagtgatgc aaaacgggct aaatttattg
    12061 ggagtctcgt cttactggca aacattgatc accggcatca tcatcgttgc cagcattagt
    12121 gccacggcgt ggagtcagca tcagaaccgg agtctgctat gaaaaaatcg tggcgtaata
    12181 acgttgagtt ttacttgatc ggcctgctgg tgctaactgt tgccgcgttc agcattacta
    12241 tgccggagat tttctggtcg ataagtaatt tccagtccgt cgcttcgcaa atgcccgtgc
    12301 tgggcattct ggcgttggct atggcggtga ccatgctttg cggtggcatt aacttgtcaa
    12361 ttatagccac tgctaacgcc tgttcactgg tgatggcgtg ggtggcgact cagtatccac
    12421 cgggtattgc tacggttgtc gctacgttac tggcgggcgc tggcgcggcg gtgataatcg
    12481 gtttatgtaa cggcgtattg attgctggta ttcgagtgtc gccaattctt gcaacgcttg
    12541 ggatgatgac gttgctcaaa gggataaata ttctggtgac aggtggtagc gccattgcca
    12601 attatccctc atgggtgctg tggttgaatc atgcgcaatg gttcggtatt ccgctaccga
    12661 tgtggttgtt tgccgcagtg gcactggggc tatggatatt gctggagaaa acgccgctcg
    12721 gtaaagccat ttacctgatt gggtctaacg aacgagcaac actttactcc ggcattaaca
    12781 ctcgtcgggt gctgatttgg gtttatgtca tttccgcctt gctttgtgcg gtagcggcat
    12841 ttttgatgat gtcgaaactc aactcggcta aggcctctta tggcgaatcg tacttgctgg
    12901 tgtcaatcct cgcggcagta ctcggtgggg ttaatccgga cggcggcagc gggcggataa
    12961 ttggtatggt gctggcgctg tttcttctgc aaattattga aagcggcttc aatattctcg
    13021 gtataagccc ctacctgacg atggccttat ggggaacgtt gctgctctgt ttcattcagg
    13081 ttcgcggcat gttggggctg gatcgggtgg tttaaatatt ggctatattc aatggacgcg
    13141 ttttgccgcg atgacatatc aggcgtcgcc aaatacacat agctaatcag gagtaaacac
    13201 aatgaagatc aaagctgttg gtgcatattc cgctaaacaa ccacttgaac cgatggatat
    13261 cacacggcgt gaaccgggac cgaatgatgt caaaatcgaa atcgcttact gtggcgtctg
    13321 ccattccgat ctccaccagg tccgttccga gtgggcgggg acggtttacc cctgcgtacc
    13381 gggtcatgaa attgtggggc gtgtggtagc cgttggtgat caggtagaaa aacatgcgcc
    13441 gggcgatctg gtcggtgtcg gctgcattgt cgacagttgt aaacattgcg aagagtgtga
    13501 agacgggctg gaaaactact gtgatcacat gaccggcacc tataactcgc cgacgccgga
    13561 cgaaccgggc catactctgg gcggctactc acaacagatc gtcgttcatg agcgatatgt
    13621 tctgcgtatt cgtcacccgc aagagcagct ggcggcggtg gctcctttgt tgtgtgcagg
    13681 gatcaccacg tattcgccgc tacgtcactg gcaggccggg ccgggtaaaa aagtgggcgt
    13741 ggtcggcatc ggcggtctgg gacatatggg gatcaagctg gcccacgcga tgggggcgca
    13801 tgtggtggca tttaccactt ctgaggcaaa acgcgaagcg gcaaaagccc tgggggccga
    13861 tgaagttgtt aactcacgca atgccgatga gatggcggct catctgaaga gtttcgattt
    13921 cattttgaat acagtagctg cgccacataa tctcgacgat tttaccacct tgctgaagcg
    13981 tgatggcacc atgacgctgg ttggtgcgcc tgcgacaccg cataaatcgc cggaagtttt
    14041 caacctgatc atgaaacgcc gtgcgatagc cgattctatg attggcggca ttccagaaac
    14101 tcaggagatg ctcgattttt gcgccgaaca tggcatcgtg gctgatatag agatgattcg
    14161 ggccgatcaa attaatgaag cctatgagcg aatgctgcgt ggtgatgtga aatatcgttt
    14221 tgttatcgat aatcgcacac taacagactg aaaaaattaa taaatacgct gtggtttaac
    14281 atattaactt cgctctccac ttaacttttt agttaaggag agcgaaataa tatcaaagta
    14341 gcagtaaaac ctataacgta
//
LOCUS       DAPPNJ010000119        14047 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000119 DAPPNJ010000000
VERSION     DAPPNJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.120"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..297)
                     /locus_tag="RPM73_002937"
     CDS             complement(<1..297)
                     /locus_tag="RPM73_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDX6521275.1"
                     /translation="MSAPATILDMCCGSRMFWFDKSDKRAIFSDIRKEGYTLRNGRRL
                     IISPDIIADFRALSFADASFSMVVLDPPHLERVGDNAWMGKKYGRLNKDAWRDDL"
     gene            complement(294..716)
                     /locus_tag="RPM73_002938"
     CDS             complement(294..716)
                     /locus_tag="RPM73_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDX6521276.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            complement(757..1722)
                     /locus_tag="RPM73_002939"
     CDS             complement(757..1722)
                     /locus_tag="RPM73_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521277.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDSLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(1703..2224)
                     /locus_tag="RPM73_002940"
     CDS             complement(1703..2224)
                     /locus_tag="RPM73_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521278.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(2208..2438)
                     /gene="dicC"
                     /locus_tag="RPM73_002941"
     CDS             complement(2208..2438)
                     /gene="dicC"
                     /locus_tag="RPM73_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HDX6521279.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGDLVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKTKRAGWLNNENHS"
     gene            2522..2929
                     /gene="dicA"
                     /locus_tag="RPM73_002942"
     CDS             2522..2929
                     /gene="dicA"
                     /locus_tag="RPM73_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional dual regulator DicA"
                     /protein_id="HDX6521280.1"
                     /translation="METKNLTIGERIRYRRKNLKHTQRSLAKALKISHVSVSQWERGD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPTPPVEKPVALSPKELELLELFNALPES
                     EQDTQLAEMRARVKNFNKLFEELLKARQRTNKR"
     gene            3096..3251
                     /locus_tag="RPM73_002943"
     CDS             3096..3251
                     /locus_tag="RPM73_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDX6521281.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3411..3629
                     /locus_tag="RPM73_002944"
     CDS             3411..3629
                     /locus_tag="RPM73_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521282.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            4197..4385
                     /gene="dicB"
                     /locus_tag="RPM73_002945"
                     /gene_synonym="ftsT"
     CDS             4197..4385
                     /gene="dicB"
                     /locus_tag="RPM73_002945"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDX6521283.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            4382..4573
                     /gene="ydfD"
                     /locus_tag="RPM73_002946"
     CDS             4382..4573
                     /gene="ydfD"
                     /locus_tag="RPM73_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDX6521284.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            4666..7137
                     /locus_tag="RPM73_002947"
     CDS             4666..7137
                     /locus_tag="RPM73_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDX6521285.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRRA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAKPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANIIQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPESVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7210..7461
                     /locus_tag="RPM73_002948"
     CDS             7210..7461
                     /locus_tag="RPM73_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDX6521286.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            7496..8776
                     /locus_tag="RPM73_002949"
     CDS             7496..8776
                     /locus_tag="RPM73_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDX6521287.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGETRQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(8778..8906)
                     /locus_tag="RPM73_002950"
     CDS             complement(8778..8906)
                     /locus_tag="RPM73_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDX6521288.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(8964..9983)
                     /locus_tag="RPM73_002951"
     CDS             complement(8964..9983)
                     /locus_tag="RPM73_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDX6521289.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(9995..11209)
                     /gene="rspA"
                     /locus_tag="RPM73_002952"
     CDS             complement(9995..11209)
                     /gene="rspA"
                     /locus_tag="RPM73_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDX6521290.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(11415..11741)
                     /locus_tag="RPM73_002953"
     CDS             complement(11415..11741)
                     /locus_tag="RPM73_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDX6521291.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            11876..12217
                     /locus_tag="RPM73_002954"
     CDS             11876..12217
                     /locus_tag="RPM73_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDX6521292.1"
                     /translation="MKITLSKRIGLLAILLSCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12252..12812
                     /gene="speG"
                     /locus_tag="RPM73_002955"
     CDS             12252..12812
                     /gene="speG"
                     /locus_tag="RPM73_002955"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDX6521293.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(12815..13525)
                     /locus_tag="RPM73_002956"
     CDS             complement(12815..13525)
                     /locus_tag="RPM73_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDX6521294.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            13633..13938
                     /locus_tag="RPM73_002957"
     CDS             13633..13938
                     /locus_tag="RPM73_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDX6521295.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         3982 a         3394 c         3154 g         3517 t
ORIGIN      
        1 caaatcatca cgccaggcat ctttattcag ccgtccatat ttctttccca tccaggcgtt
       61 atcaccaaca cgctcaagat gcggagggtc taatacaacc atcgaaaaag atgcgtctgc
      121 aaatgataat gcacgaaaat ctgcgataat gtcagggctg ataatcaagc gtctcccatt
      181 gcgtaatgtg tatccttctt ttctgatatc gctgaatatc gcccgtttgt cagacttatc
      241 gaaccagaac atgcggctgc cacagcacat atcaagaatg gttgccggtg cactcactgc
      301 gccacgtcct gaaaattacc ctgatagaaa gccagtatgc gctgcataac ttcgctcttc
      361 cggcactcgc gacagattat gtttaggcga ctgtcgtagc gacgtatttc tccgtcaggt
      421 gatgaccaga taaggtccgg atcaaccaca gcaggtttct tcacctttgc cctcgagagt
      481 tttttgcggg cgttttgcca gtccttacgc gcctgttcag aggggaataa cccgtagccg
      541 gagttgtata catcgccact ggctaccagc tctctggcaa gaacgctcat cagatatcta
      601 gtcgcacctg ttttagcttc cagttgccgt aacgtctcac ggccgctctg gcgcacgagt
      661 tccaccacct gccatttaat tttttcccgc tcttcttgtg taaaaacttt tgccaccagt
      721 cccccttaaa attacctcat gacctgaaat caacacttat cctctgaaac caggcggaat
      781 ttctgtatcc ggttcagaaa tatgattaac acaacgctgt acaggtgaac gtcccaggcg
      841 gatgaccagt tcgtcccatt tttcgcggag ctttgacggg ctcatgatgt tttttaccca
      901 gaatggatct cgctgaaccc gaccaaacat ttcgcaaatt tgtctgtggc ttctgccatc
      961 cagcatccgc attgtgcgca cgtcattggc ccaggcagtc cagttaggct cttttggtcg
     1021 catgatctcg ccatcatcac tggctgcctg ttcgtagaga cccacgatcc gcccccagat
     1081 ccactgcgca cacgccaaat cctccctgct accccactgg cgttttttcg cactaaacac
     1141 aaccgcgtcg gagttccggg ttaaagaatc ctgttcagtc gtctgcgggt ccggttgcga
     1201 agcttccgga cgaaaagtgt ttttattctc tgtagtaatc tctgttgtat tctctgtaag
     1261 atcatcaggc cattttgacc cgatgacatt gggtcgtttt gaaccaatgg agcgtttcat
     1321 tttgacctct tccatcgtgt cattttgacc tgatggagcg gcgcattttg aaccgatgga
     1381 ttcgctcaat ttgccaccat ctaaaagctc gctcccatag ttgatcgtgt agaaattggt
     1441 catatcgcgc tttgatttat tgagcttttc acaacgcaaa agccccagcg ttttcagact
     1501 tgcaaacgcg cgctttaacg ttgactctga ccagaatggg aactgttcca gccattgttc
     1561 cgttgtgtta taaatccagc gaacaccatc acattccatg ccggagttgg tatctctcaa
     1621 ccagtagtgc agttgttgca aaacaatggc ttcgtttaag ccaattttca ttgccagctg
     1681 cgtgtttata accagtgggc gttcagcaaa aagaaggctc ataattccat ccagcttttt
     1741 gttggtattg ctgtcgatac gcaagcttga aagcaattgc tttttctata agttcgtcag
     1801 tttcacgatc cactacggca ggatcagcaa aaagcagtcc ggactccacc acatcgccat
     1861 attctttgtt taacccggcg atcatgcacg tgatgctttt tccgtcagta atttcacgat
     1921 acaacctgaa atcactaatc cggatagcct ccataatcgc aggcactagc gccgtgaact
     1981 tttcacgctt atccctggtg tcgatagcct tccagcgttc gaatatcttc actcgattaa
     2041 caccaagcgc tcgctgatca accgcgccac cttcatctgt gacacgctga acatcgatgt
     2101 tcgggcgctc tttcaaagcc cagaatgctt cagtgattaa tatcgtcgcc tgctcctgtg
     2161 tcattcctgg tcgacatatc caggcatcca gagcctcacg agcctgttca ggagtgattt
     2221 tcattgttca accaccccgc ccgcttcgtc ttacgatatt catcataaac tttgggatca
     2281 tactgaagct ccccgccaga tgcctcctgt agacgcatcg cgcgaccttc gggaactaaa
     2341 tcccctttcc agctataaag cgaagccaaa cgaatacctg ctgcttgtgc aagttttgtt
     2401 tttgaaccga aatacaaaag agcgtcagtt ttaagcattt aaaacacctt tattgttagc
     2461 cataactaac aagatagatg ttaacaaaaa catagtcaat acgatttagc attagctaac
     2521 tatggaaaca aaaaatttaa ctatcggcga acgcatcagg tatcgtcgga aaaacctcaa
     2581 acacacccaa aggtctcttg ctaaagccct gaaaatctcc catgtgtctg tatcacaatg
     2641 ggaacggggt gatagtgaac ctacagggaa gaaccttttt gccctcagca aagtattgca
     2701 atgctcacca acatggattc tatttggcga tgaagacaag caaccaacac cacctgttga
     2761 gaagccagtt gccttatccc ccaaagaact agagctcctt gagctgttta atgcactgcc
     2821 agaatcagaa caggataccc agctcgccga aatgcgagct cgagtaaaaa acttcaataa
     2881 actctttgaa gaattactaa aagcccgtca gcggacaaat aaaagataac atcatcaatg
     2941 agttatcttt taccacatca attatgttag ctatagcata caaaatcact tgaccgatat
     3001 gttagtcatg gctaatcttg tttgcatcaa cacaccgcac ggtgttctca gcaaacagtt
     3061 ccgctacccc agcgttaagg ggaaatgagg tccgcatgga tactatcgat cttggcaaca
     3121 acgaatctct ggtgtacggc gtgtttccaa accaggacgg tacgttcacc gcgatgacgt
     3181 ataccaaaag caaaacgttt aaaaccgaaa atggtgcccg tcgctggctg gaaagaaact
     3241 caggtgagtg atatggattt cgacacaatc atggaaaaag cttacgaaga atacttcgaa
     3301 ggtcttgccg acggcgaaga agctctcagc ttcaacgaat ttaaacaggc gctttccagt
     3361 tcggcaaaat ctaacggctg ataagcgaaa cagcaccgcg aggaatcagt atgcagaaac
     3421 gagaacccgt catcatcgcg ccagactata ccgatgatga actttatgag tggatgcgcc
     3481 agaaaattaa tgcagcgcag gatctgaaat gggccaatga agccagggct aagcaggctg
     3541 aaaatctgtc cgctctggag caggatatca ccaatctgga aaaagcagcg gcattaagca
     3601 ttgccagaat gattacatac ccgcgttaat agctaaccaa cgaagctaag gttggtaatt
     3661 aaggagttct ccacgggtga ggtggagtgc gtgcgccgga cacgggtgag catccggcac
     3721 tgacagttta ctgaaaggat atttccctga aaagtcagac cataacgcga aagcgcacgg
     3781 cgaggtagct ggttcataga tagcctgtcg ttaaattttc gtcgaccgtg cgcttccggt
     3841 tgtggcaacc cgcgaaatgg tgcggcggta agtatggcgg ggttatttct tccccgttga
     3901 ggacaccggg ttgtcaggtt gaccatacgc ttaagtgaca accccgctgc aacgccctct
     3961 gttatcaatt ttctggtgac gtttggcggt atcagtttta ctccgtgact gctctgccgc
     4021 cctttttaaa gtgaattttg tgatgtggtg aatgcggctg agcgcacgcg gaacagttaa
     4081 aaccaaaaac agtgttatgg gtggattctc tgtatccggc gttaattgtt aactggttaa
     4141 cgtcacctgg aggtaccagg cactgcatca caaaattcat tgttgaggac gcgataatga
     4201 aaacgttatt accaaacgtt aatacgtctg aaggttgttt tgaaattggt gtcactatca
     4261 gtaacccagt atttactgaa gatgccatta acaagagaaa acaagaacgg gagctattaa
     4321 ataaaatatg cattgtttca atgctggctc gtttacgtct gatgccaaaa ggatgtgcac
     4381 aatgaattca gcatttgtgc ttgttctgac agtttttctt gtttccggag agccagttga
     4441 tattgcagtc agtgttcaca ggacaatgca ggagtgtatg actgcagcaa ccgaacagaa
     4501 aattcccggt aactgttacc cggtcgataa agttattcac caggataata tcgaaatccc
     4561 ggcaggtctt taaaacagtt ccgtaataaa tatccggttt cattcttata tgccagcaat
     4621 ggcagggatt tgttcatcct taaatctgtc atgaggttaa aacaaatgag taaagtcttt
     4681 atttgcgccg ctattcctga cgaactggca acaagggaag aaggcgctgt ggctgtagcc
     4741 acagccattg aagctggcga cgaacgccgt gctcgagcaa aatttcactg gcaattcctg
     4801 gaacattatc cggctgctca ggactgcgct tataaattta ttgtctgcga ggataaacct
     4861 ggcatacccc gccctgccct cgattcatgg gatgctgaat atatgcagga aaaccgctgg
     4921 gatgaggagt ctgcttcttt tgtcccggtt gagactgaat ccgatccgat gaacgtcact
     4981 tttgacaagc tggcccctga agtacagaac gctgtcatgg ttaagttcga cacatgtgaa
     5041 aacatcaccg ttgatatggt tattagcgca caggaattgt tgcaggaaga catggcaaca
     5101 ttcgacggac atatcgttga agcgttgatg aaaatgccag aagttaacgc catgtatccg
     5161 gagcttaagt tgcacgccat tgggtgggtt aagcataaat gtattcctgg tgctaaatgg
     5221 cccgaaattc aggcagagat gcgcatctgg aaaaaacgtc gcgaaggtga acgcaaggaa
     5281 accggaaaat acacgtctgt tgttgatctc gcccgcgcca gagccaatca acagtacact
     5341 gaaaattcaa caggaaaaat cagcccggtc attgctgcca ttcatcgcga atacaagcag
     5401 acatggaaaa cactggatga cgaactggcc tacgctctct ggcctggtga tgtggatgcc
     5461 ggaaacattg acggcagcat ccatcgccgg gcaaaaaatg aagttatcga caacgaccgc
     5521 gaagactgga agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct tcgctacgac
     5581 cgccaaacta tttttggcct tgtccgtgag cgtccgatcg acattcacaa agatcccgta
     5641 gcactgaaca aatatatctg cgaatacctg acgacaaagg gcgtgtttga gaatgaagaa
     5701 acagacctgg gcactgttga tgttctccag tcatcagaaa cacaaactga tgcagtggaa
     5761 actgaggtat ctgatatccc aaaaaatgaa accgcgccgg aagctaaacc atctgtagag
     5821 cgtgaggggc cgttctattt cctcttcgca gataaggacg gagaaaaata cggtcgcgca
     5881 aacaaacttt ctggtctgga taaggcactg gctgctggcg ccactgaaat cacaaaagaa
     5941 gaatattttg cccgaaaaaa tggcacatac acgggcttac cgcaaaatgt agataccgct
     6001 gaagattcag aacaaccaga gccgataaaa gttaccgctg acgaagtaaa caaaattatg
     6061 caggcagcca atatcatcca gcctgacgcc gataagttgc ttgctgcatc acgtggtgaa
     6121 tttgttgaag ggattagtga cccgaatgat ccgaaatggg ttaaggggat ccagacccgc
     6181 gattctgtga accagaacca gcatgaatcg gaacggaact accaaaaagc ggaacaaaac
     6241 agcccaaatg cgttacaaaa cgagccagaa acgaaacagc ctgaatcagt ggcgcaacag
     6301 gaagtggaaa aagtctgcac cgcctgcggt cagaccggcg gcggcaactg ccctgattgt
     6361 ggcgcggtaa tgggcgacgc aacataccag gaaacattcg atgaagagta tcaggttgaa
     6421 gttcaggaag atgatccgga ggaaatggaa ggcgctgaac atccacacaa ggagaacact
     6481 ggcggcaatc agcatcacga tagcgataat gaaactggcg agacggcaga tcactcaatt
     6541 aaggtgaacg gtcatcaaga aatcacatcc accagcagga cgtgtgacca tctaatgatc
     6601 gaccttgaaa ccatgggaaa aaatcctgat gccccgatca tctcaatagg tgcaatattt
     6661 ttcgatccgc aaaccggaga tatgggaccg gaatttagta agactatcga tctggaaact
     6721 gctggcggag tcattgatcg ggacaccatt aaatggtggc ttaagcaatc acgcgaagcg
     6781 caatctgcca ttatgaccga tgaaatcccg ttagatgatg cactgttaca attgcgggaa
     6841 tttatcgacg aaaactccgg tgaatttttt gttcaggtct ggggaaatgg agctaacttc
     6901 gacaacacga ttttgcgccg ttcatacgaa cggcagggga tcccctgccc gtggcgttac
     6961 tacaacgatc gcgatgtacg cacaatcgtt gagctgggga aagccataga cttcgatgcc
     7021 agaacggcta ttccattcga aggtgagcgc cataatgcac ttgatgacgc ccgttaccag
     7081 gcaaaatacg tttcagttat ctggcaaaaa ctgatcccga gtcaggctga ttcttaatgt
     7141 tcaactgtcg ccggttgtga ctggtattct gcaaccggcg ctcgtctgat gtaagagata
     7201 aagaaatcga tgagcgaagt aatcatgatt gtctctcccg gcaaatgggt atccgaagag
     7261 cagttaattg cgctgaaagg aataaaaaaa ggtacgttaa aaaaggcccg ggaaaaatcg
     7321 tttatggaag gaagggaata taagcatgtc gctcatgacg gtatgccatg ggataacagt
     7381 ccatgctttt acaacctgga agaaattgat cgctggattg agcgccaggc atcagcaaga
     7441 ccaagacgtc atcttacttg actaaaagcc acactaacta atgagagaaa ttgaaatgaa
     7501 atatccgaca ggcgtggaaa accatggagg gaaattacgt atctggtttg tttataaagg
     7561 cgtaagagtc agggaaaacc tgggggttcc tgacacagca aaaaacaggc gcgttgcagg
     7621 tgagctacgc gcctctgttt gttacgcaat aaaaactggt gttttcgact atgcaaaaca
     7681 gtttccctcc tcacgcaatc tggaaaaatt tggtgagacc cgacaagatt taaccataaa
     7741 agaactggct gaaaaatttc tggcactgaa agaaactgaa gtcgcaaaaa catcactcaa
     7801 cacataccgt gccgtcatca aaaatatcct gagcataatc ggtgaaaaaa atcttgcctc
     7861 atcgattaat aaagaaaaat tactggaggt tcgtaaagag ctactgactg gataccagat
     7921 ccccaaaagt aactatattg ttacacaacc agggagatcg gctgtaactg taaataatta
     7981 catgacaaat cttaacgccg tgttccagtt tggtgttgat aacggttacc tggcagataa
     8041 cccgtttaag gggatctcgc cattaaagga atcaagaacc attccggatc ctctttcgcg
     8101 ggaggaattc atccgtctta tcgatgcgtg cagaaatcag caagccaaaa atttatggtg
     8161 tgtttctgtt tatactggcg ttcgccctgg tgagctgtgt gcacttggat gggaggacat
     8221 agatctgaaa aatggaacaa tgatgatcag gagaaattta gcaaaagacc gtttcacggt
     8281 accaaaaaca caggcgggaa ccaatcgggt cattcatctt attaagccag caatcgacgc
     8341 tctccggagt cagatgacat taacgagact gagcaaagag catatcattg atgttcacct
     8401 cagagagtat ggcagaacag aaaaacataa atgcaccttt gtttttcaac ctgaagtgtc
     8461 agcgagagta aaaaattatg gtgaccattt taccgttgac tcaataaggc agatgtggga
     8521 cgcagcgata aaacgtgccg gactccgcca tcgaaaatca tatcagtcga gacatactta
     8581 tgcctgctgg tcgctgacag ctggtgctaa cccggcattt atagcaaacc agatgggcca
     8641 tgcagatgcg caaatggtat ttcaggtata cggaaaatgg atgtctgaaa acaataatgc
     8701 acaggtagct ttgttaaata cacagttaag cgagtttgcc ccaaccatgc cccataacga
     8761 agcaatgaaa aattaattta atatttatca aatagttgac acgtatgact cttgaaatcc
     8821 ataaattcaa gcgcagtgcc cagccatccc gatactgctg ctttcaccaa atccttagtg
     8881 cttctttcat gtttttctat tgtcataatg gttatctcta aaaaagaggt aagatgcgta
     8941 ctacttactc gccgttattg gtattattca gaaaaagtga gtaagacttt gcagcaatgt
     9001 ttttgatcct gttcaaataa actaatggca tcagcaacat gctggaaatc aaacgtatgg
     9061 gtaattaatt tttctggttt aattaaccct ttacttaacc agtcgataac aaccggaaat
     9121 ttatttgcat ttaagcgtga agagaaaata gagagttctt ttccggtaat tccttgctga
     9181 atcacttcag acggttcact ggagaagccc atcaatacaa tacgtgccgc tggagaagcc
     9241 agcgttacgg cctctttcag gatagaagga tgacaagccg catcgataat taatgtcggc
     9301 ttgatgcctt tttcagcgaa aatctcgcca agcggtgtct ggctgttatt aatcgccctg
     9361 tctgccccgc tctctttcgc tttttccagt cgttcatcaa tgcgatcggc aacaatcaca
     9421 tttttaacgt tatagacgcc ttttaatacc tgaacgatcg tcaggccgat tggaccggca
     9481 ccataaacca gaacggtatc attttcagtc ggttgaccat gtccggttac gttagccgca
     9541 atggtaaaag gttcgatcat caccgcatat tgatcggcca ctgcttcagg aattttccac
     9601 gcattttttg ccggaaccac ggcatattca ctgaaaccac cgtcagcgtg cacacctaat
     9661 acagcaagtg tcgtacaaac gttcggttta cctatagagc acggatagca atgcccacag
     9721 ctgaccaccg gatcgacagc aacacgttca ccgactctgg cgctttccac gccgtcaccc
     9781 accgcatcaa tgacgccaaa gaattcatgt ccaatgacgc gcggatattt cgcaaaagga
     9841 ttatgcccac gataaatatg gctatcagaa ccacaaattc cggcaagttt cacttttact
     9901 cgtacttcac ccgctgacgg ggtgggtatt tcacgttcga caatcgccag ttgattcggt
     9961 ttttcaatta atatgctttt cattatctta ctccttacca gttccacagc gtgccatctt
    10021 ccagacgtgc gactggtaga taagcaggtt catagggata tttcgccgcc agcttttcat
    10081 cgaattcgat gccaagaccc ggtttgtctc ccggatgcat atagccgtta tcgaaagtcc
    10141 agttgtgcgg gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga
    10201 aattggggac ccacagatca aagtgcagcg ctgcagccat gcagactggt gacaaatcgg
    10261 aaggaccgtg tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca
    10321 taccggtaat tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt
    10381 caatcagttg tttgcagtcc cagatgctgt tgaagacttc acccactgcg atgggtgtga
    10441 cggtatgttg gcgaatgaga cggaagcatt cctggttttc tgcaggcgtc gggtcttcca
    10501 tccagaacat gcgataatct tcaatgcttt taccaaagcg cgccgcttca ataggcgtta
    10561 agcgatggtg catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt
    10621 caaacaattt cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc
    10681 actgtccttt ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg
    10741 ttttcatacc agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac
    10801 gggcataatc atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca
    10861 ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg catgttggca gctttggctt
    10921 taatatccca cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac
    10981 gccagtaagc acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac
    11041 caataagctg cggacaaagg tgatcctgca aataagaggc cacggaaagc tcacgtccat
    11101 tgagggtggc atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga
    11161 aattacgccc cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct
    11221 tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag ctactaccat acaagtataa
    11281 agatcgaaaa aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg
    11341 acaaaagatg aaatagaata ccttttgtca gctgacactt cctcttatct tattgataaa
    11401 atggatttat gttcctacgt gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc
    11461 gcaatcaacg cacccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc
    11521 cacatcaacg ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc
    11581 gctggatgca acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac
    11641 agccagatac tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca
    11701 cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca tctttgtctc ttattgacat
    11761 catgtatagt tatagggcga cataatatca tcaatataaa caccctcctg gtacgttttg
    11821 cgtccgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
    11881 aatcactctc agcaaacgaa tcggcctgct cgctattctg ctgtcttgcg cactggcatt
    11941 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
    12001 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
    12061 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
    12121 cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg gagcccaata ctttgcgctg
    12181 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
    12241 aaggacacgt tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt
    12301 tacgctatgt acatcaactc gacaacaacg ccagtgtgat gcgttactgg tttgaggaac
    12361 cctacgaagc ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg
    12421 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
    12481 ttaaccatgt tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga
    12541 aaggtctggc aacccgtgcc gccaaattag caatggacta tggctttacc gttctcaatc
    12601 tctataagct gtatctgatc gttgataaag agaatgaaaa agcgattcac atttaccgca
    12661 agcttggctt ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc
    12721 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
    12781 gtcagactct cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga
    12841 tggtgtaaac ccgttcgacg gcgggtttta ctccttcatc aacaatgatt agctgacagt
    12901 ccaccggatt agcgtgactg tcatattcac agctctgttt tacattacca acccgttgat
    12961 tattcagtaa agtaaccgct gtgtaatcta attttttgat cggatccgtt gatggcgtgg
    13021 cgctgacaga taatgttttg tcgttacttt tcgtggtttt acccagcgga taaccctgat
    13081 catcatagcg atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat
    13141 catcggtttc ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta
    13201 aacgtactct cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag
    13261 cgaccacggt attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg
    13321 acaaagtccc agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga
    13381 aatcttttac cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct
    13441 caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta
    13501 agcatggtag taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca
    13561 tagtagcaaa tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt
    13621 aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca
    13681 cccgcggtac tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc
    13741 attatcaata atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag
    13801 gttgatcagc ctgattccca ggtcgtcggg cattgtgcta atgatacgca taaaattctc
    13861 tacacccgca ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg
    13921 cctgccgaac cgcagtaata cattatcgac tgaacgccgg atacgacaca tccggcgatt
    13981 tgaacataca acataatccc accttattac tcataccctt ctattgatat ggattaataa
    14041 ttcttaa
//
LOCUS       DAPPNJ010000120        13907 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000120 DAPPNJ010000000
VERSION     DAPPNJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.121"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..883)
                     /gene="yhaJ"
                     /locus_tag="RPM73_002958"
     CDS             complement(<1..883)
                     /gene="yhaJ"
                     /locus_tag="RPM73_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDX6521296.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKL"
     gene            988..1689
                     /locus_tag="RPM73_002959"
     CDS             988..1689
                     /locus_tag="RPM73_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDX6521297.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1712..1876
                     /locus_tag="RPM73_002960"
     CDS             1712..1876
                     /locus_tag="RPM73_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521298.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2088..3398)
                     /locus_tag="RPM73_002961"
     CDS             complement(2088..3398)
                     /locus_tag="RPM73_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDX6521299.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3426..4757)
                     /locus_tag="RPM73_002962"
     CDS             complement(3426..4757)
                     /locus_tag="RPM73_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDX6521300.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5032..6396)
                     /gene="tdcG"
                     /locus_tag="RPM73_002963"
     CDS             complement(5032..6396)
                     /gene="tdcG"
                     /locus_tag="RPM73_002963"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDX6521301.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6468..6857)
                     /locus_tag="RPM73_002964"
     CDS             complement(6468..6857)
                     /locus_tag="RPM73_002964"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDX6521302.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6871..9165)
                     /gene="tdcE"
                     /locus_tag="RPM73_002965"
     CDS             complement(6871..9165)
                     /gene="tdcE"
                     /locus_tag="RPM73_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDX6521303.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9199..10407)
                     /gene="tdcD"
                     /locus_tag="RPM73_002966"
     CDS             complement(9199..10407)
                     /gene="tdcD"
                     /locus_tag="RPM73_002966"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDX6521304.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10433..11764)
                     /gene="tdcC"
                     /locus_tag="RPM73_002967"
     CDS             complement(10433..11764)
                     /gene="tdcC"
                     /locus_tag="RPM73_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDX6521305.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11786..12775)
                     /gene="tdcB"
                     /locus_tag="RPM73_002968"
     CDS             complement(11786..12775)
                     /gene="tdcB"
                     /locus_tag="RPM73_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDX6521306.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12874..13812)
                     /gene="tdcA"
                     /locus_tag="RPM73_002969"
     CDS             complement(12874..13812)
                     /gene="tdcA"
                     /locus_tag="RPM73_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDX6521307.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPTVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3610 a         3769 c         3269 g         3259 t
ORIGIN      
        1 aaagtttggg aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc
       61 aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt
      121 ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta
      181 atgcctgacg tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct
      241 gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca
      301 cgcgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa
      361 cgtagacgtt cattaaggta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat
      421 gcatatccgg cgcgataacg atatccgccc gcccctgctc cagtcgttcc cacgcccccg
      481 ccagcacttc ggtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta
      541 acgggaaaaa ggcaggtgtc ggtaccagcg cctcggtcac aatggtgaga tgcgtttccc
      601 aaccgcgcgc gagggcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc
      661 gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt
      721 caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag
      781 gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca
      841 taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga
      901 aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata
      961 aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag acagtgtgga
     1021 caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac
     1081 ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt
     1141 gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga
     1201 gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg tgaggcgttg
     1261 ctgctctcta cccagccggg tgtcagctac agcgaacaca atctcagcaa agacaaaccg
     1321 ttaacacgaa tgcagctttg gctggacgcc tgcccgcagc gagagaatcc gctgattcaa
     1381 aaactggcgc ttaatatggg caagcagcaa ttaatcgcct cgccagaggg tacgatggga
     1441 agcctgcaat tacgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt
     1501 gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat
     1561 gcgcttacgc atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt
     1621 gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg
     1681 cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca
     1741 gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga
     1801 agagatgttg agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga
     1861 tgaagctacc gcgtaaagac aggttgccgg atgcggcgaa aacgccttat ccggcctaca
     1921 aaatccaaca acattaacga attaaacaac ccgtaggccg gataagatgc gccagcatcg
     1981 catccggcat caggtacaaa acacaacaac atcaacaagt tgagcacccc gtaggccgga
     2041 taaggtgcgc cagcatcgca tccggcacga tacccaaaac ctggcgttta tctggccttg
     2101 ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca
     2161 cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt gccggtcacg
     2221 gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc
     2281 ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc cgctgacatc gccgatcata
     2341 ctgctgatcg ccatcgagat ggtttcataa cgcccatcca ccagccatgc catcccggcg
     2401 gcggccccca ttgctgcggt cgttgcggca cacagcgcag acaaacgcgg taactggtta
     2461 tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac gcgccagccg ttcatcatcc
     2521 gctccgaagt gttctgctac caccaccaca ggcattgttg cggtgatccc ctggttaccc
     2581 gagccggagt tactcatagc cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg
     2641 ctggtacgaa tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt
     2701 ttttccagcg tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc ctgcgataac
     2761 gcacaattta gcttcgcgga atcgagaata aagcggatcg ccgcaaacgg gacttcattg
     2821 acgaacttca ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc
     2881 tcgccctctg ccacacacgc ctgctgggta aacaccacac cattgtgcgt ctcaatatgc
     2941 acaatgttgg tatgcccgcc gacgatggtg acacacgccc acttctcacc gttccagact
     3001 ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga tcttaacgga gactttcccc
     3061 gccgccagca gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc
     3121 agcccggcgt tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg cagccccacc
     3181 attcccgtgc cgggaacggt gacgcccaga ccgttcttca tcagatttgg cgaaacccag
     3241 gcttctacac gttcaaccgg accttccagt tctgccgcag caaccgccgc cgccagcgcc
     3301 agtgaaatcg gttcagtaca tcccagcgcc ggttttactt cctcctgaac ggcgaggatg
     3361 taacgctgcc ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag
     3421 gtaaatcaag aaaatgccag gaacggagaa acacaaagca acaaaccagt gacgataatc
     3481 aggtacagag acatcccttt gtatttgtgc aatgccggta ctttgtaaac cagccacgcc
     3541 gggatcaggc accctaccat gccgaaaatc gggctacaga tagaggtgaa gctcaacacc
     3601 ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc
     3661 tgaacaagat tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg
     3721 atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt catgacagca
     3781 aagatattga gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac
     3841 tgtgcggcaa tcgccagcgc ggaaatattc tgctcatagg cttttaccgc ttcgtcatgt
     3901 cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac
     3961 gcgatattca ttgcccgcaa tgctttatgc cgcgccactt caattgattt ttcccgcgag
     4021 cgataagaga tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt cagggtaaac
     4081 ggcagcgtaa taatggcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta
     4141 tacagatgcc acattcccac catcgacacg cccagcgccg cgaccaccag cagcttggtc
     4201 agcaccatgc cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga gatcgccacc
     4261 agaatgcaaa tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg
     4321 ccgaaggtat gcagatagga agcactatcg ttggtgatgg cggtggaata gacgaacatc
     4381 caaatcacca gcattacgaa atagagcgca cctaacagga tgccccagtt tttacctaaa
     4441 taaccgctaa tgacactcgg gtaatcttta cattctggtg attctgccag cgtattaata
     4501 aacaaccgct gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc
     4561 cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc
     4621 ccgatactca taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac
     4681 tcgctctcac tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta
     4741 ttcgatgcaa tttccataat ttctcgctca atattttgta gggcttatta tttttttccg
     4801 agccgcatca aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa
     4861 tgatacgcgc gaggtcagag aaaatcttca caatcaagac tttgaaataa cggagatgga
     4921 taagaatttt ctactaaatt aatcgcaaga gaatattcat tctctatttg cggcagatca
     4981 caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca
     5041 gaccacttta atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt
     5101 gccggtttca tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg
     5161 gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat
     5221 gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt tatgctccat
     5281 cgcgatttcc gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc
     5341 tgccgccgcc attgaacagg ccacgccaat ctccccctga cagccgacct ccgcgccaga
     5401 gatggaggcg ttcattttat acagcgcgcc aatagccccc gctgccagaa aatagcgggc
     5461 aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa taagccagca ctgccggaat
     5521 aatgccgcac gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc
     5581 actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat
     5641 gttatcgcta gaaaccagct gacgacgcag cgctacggca cgacgcggca cattgagcgg
     5701 accaggcagc acgccttcag tgttcatccc gcgttcaata ccgtcatgca tcacttgcca
     5761 gatacgggta aaccctgcgt caatttccgc tttactgcgc agcgctagct cgttgtgcat
     5821 catcaggcca gatatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga
     5881 gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac aggccgaagt gttcttcttc
     5941 gacaataaac ccgccgccga cagagtaata ggttttactt aatagcgctt cctgtccctt
     6001 ccaggcagtg atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat
     6061 gttctttgct acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct
     6121 gcgcgttact aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt
     6181 tcctgccagt cccatgataa tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa
     6241 cgacccatac agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag
     6301 ccgatcaata aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc
     6361 aatcccaatt ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc
     6421 agtgaagtgg agcggaacga ccttgcgacc gtcccgctca cgaggcttta cgcactacgt
     6481 actgcgatag cttcaatttc cagcttcaca tctttcggca aacgcgcaac ctgcacacag
     6541 ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact gcttatagac ttcattgatg
     6601 gtggcaaaat cattcagatc ggtgatgaac acggtcatct tgatgatatc gcccacgctc
     6661 agcccggcag caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc
     6721 acatcagccg ggatctcacc ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc
     6781 atgctgccta aatcaacgcc ctgaacataa gggccgattg cgcctggggc acgttgcgtt
     6841 tcgataatct ttttcataca tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat
     6901 aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg
     6961 gattgtcagg ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt
     7021 cattacgttg acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc
     7081 atccagcagg ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg
     7141 aacgattgag aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac
     7201 cgacgtcaat gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg
     7261 cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta ccggttttct ggccgtacac
     7321 cacgttagaa gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag
     7381 cgctttaatt ttcttcataa agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc
     7441 gtcgttgttg ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt
     7501 ttcgtcacgg attggtttca cgcgggcata tttgatggca gacagggagt ccgtcgccac
     7561 cgacaggccc gcgatgccgc atgccatagt gcgatagaca tcacgatcgt gcagcgccat
     7621 cagcgaagct tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat
     7681 gtactgcacc gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc
     7741 cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc
     7801 caccccgccg ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa
     7861 ctgcatttgc ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct
     7921 gttgaagtca gtacgcatca gatcgtcatt ttcatactgc aaggaagagg tgacgatcga
     7981 cacctgcgcg gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag
     8041 gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt
     8101 ggtcaccagc gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg
     8161 gtcgccggag aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt
     8221 catgatgaag tgatcgatca gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa
     8281 gtcgcgctca atgtagatat cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc
     8341 attttgcgat ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgcgc
     8401 attctgcgcc gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa
     8461 cagcgcatga cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc
     8521 gcctttttcc agacgagact ggagatcggc aaattgcagt tcgcgttcac gtaccagata
     8581 actgatgcca tacagcgcta cgcggcgata gtcaccgata atgcgcccac ggccatagcc
     8641 atctggtaaa ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac
     8701 atcaaatacg ccctggttat gggttttacg cagatcggta aacagatatt caaattcact
     8761 gtccatttca cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg
     8821 gtgtagcgca cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg
     8881 gttaatatat cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac
     8941 cggcgcgtgg gttgcgtttt cgatacggat accttccatt actttttccc acaattccgt
     9001 ggtggcaggc gtcgcttcgg cgaggaaaga ttcatcgcct tcatacggtg tatagttatg
     9061 ttgaataaaa tcgcggacat taatttcgtt tttccagtcc gtacctttaa agccaagcca
     9121 tgcgtcggcg tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc
     9181 tacaatactt caactaaatt atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat
     9241 ggcatccaaa gcaatcattt tttcttcgtt agtcggaata acggcacaaa tgacacgcgc
     9301 attttcactg gaaacaattc gctctccaaa agagttagat cgattattca tttctgtatc
     9361 aatcactacg cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt
     9421 ctctcctatt ccgccggtga atataattcc atccaggcga cgtaatgaag ctgcgtgtcc
     9481 ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg
     9541 accttcatgc caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa
     9601 taatcccgac tctttattca ctacgcgttc caggtcaccc aagctctggt tggtttggct
     9661 ggcgacccag gacatcgcac caaagtcgac atcgccactg cgggttccca tcatcaagcc
     9721 ttccagcggc gtcattccca ttgaagtatc aacactctga ccgttgcgaa ccgcgcagat
     9781 tgacgcgcca ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag
     9841 caaagaatgt gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg
     9901 tacacctaac tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat
     9961 cgtctggtgg aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg
    10021 cgccgattca ataccactta aattggcgta attatgcagg ggtgccagtg gagaaacgcg
    10081 acggatatta tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc
    10141 gtgagcgatg cggtggccaa ttaaggccac actgtcattt aaattccgtt tttccagttc
    10201 aaatgcaatt gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc
    10261 cccatttacg gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa
    10321 tacttcacag tcgctggcat cgagcacgga gaacttaatc gaagacgaac cacagttaat
    10381 aaccaaaaca accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca
    10441 gtttgtatac gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca
    10501 gacgaccacg gtatttcgcc agagacggcg ctttacggat ggcatacatc ggcaacaggc
    10561 acagcaggga tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt
    10621 tggcgtaggc aacaacccag gtggagccca tgatgaagat catgctgata gtgttcagtt
    10681 tacccagcga cactttagtt ttgtcgcctt tataaccaaa cttcaggacc aggccattca
    10741 gaccttccag cgttcccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga
    10801 tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg
    10861 caaagtggtt agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt
    10921 tggccggaga caacgtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca
    10981 gcatgctggc acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt
    11041 ctttctcata ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa
    11101 aggagaaaac catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg
    11161 ttaacgacag cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca
    11221 gcgacagaga aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt
    11281 taaccatcag atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg
    11341 ccacaaagcc gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg
    11401 tattggtaat agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga
    11461 acgtgataac cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc
    11521 cggaagggtt agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga
    11581 tggggtatgc caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga
    11641 tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg
    11701 tatctgattt acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac
    11761 tcatatccta tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc
    11821 gagaaagatc gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt
    11881 ggtctaattt accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga
    11941 ctttattgcg ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca
    12001 cgatgtcatc gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat
    12061 cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag
    12121 aagccgccat gccgtgaacg ttttcagact gtacgccaat aacacgaatg gtcgggttaa
    12181 tagatttaat tgccaccgca ataccagcaa ttaaaccgcc accaccaatt ggcacaatca
    12241 cgttatcgac atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa
    12301 tcactttcgg atcatcgtaa ggtgggataa aaatacggcc ttccatttcg acaatttcgc
    12361 tcactttagc gatagtgtcg ttgaaattat caccatgcag aacgacttct gcggagtagt
    12421 cgcacgttgc cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga
    12481 tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca
    12541 ccacgccttt gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa
    12601 ttttaaatga acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac
    12661 gttcactaaa atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt
    12721 gtttcgcttc aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa
    12781 aacctcttcg cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag
    12841 gtggattatt tatgtttaaa taaaaacaaa taactaacca acttctatta attgccttcg
    12901 tctacaccca ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt
    12961 tttaatacga taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc
    13021 aaccggaata gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac
    13081 agttaagaaa tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac
    13141 gatgttttca atactgatgc catttctttg taacgtagta agaagctcgc tgtagtaccc
    13201 catattggtt tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc
    13261 ggtgcatgtt cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac
    13321 atgtaaatcc tgaagcttca tttctgcgct taacgtacca atcgcaaaat ccaatcgacc
    13381 gtcgcggatt gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt
    13441 cggaaacacc tctttgaact tgttgatcat cccggacata aaagtaaaac caatcaatga
    13501 aggaaaacca aatgagactt ccaccaccgc ctcagaagac ataccgctta tctcattaac
    13561 catatttttc atttcacggg taatggattc ggaacgagag agtaacagtt gaccggctgg
    13621 tgttaatgtt acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat
    13681 atcgttaatg attttactga cggtcggttg agttaaccct aattcttttg cagccgagcc
    13741 gatagagcca cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag
    13801 aatagtgctc ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat
    13861 gaaatgaaca ggatatgtgc gaccactcac aaattaactt tcaatac
//
LOCUS       DAPPNJ010000121        13605 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000121 DAPPNJ010000000
VERSION     DAPPNJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.122"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            681..2609
                     /gene="thrS"
                     /locus_tag="RPM73_002970"
     CDS             681..2609
                     /gene="thrS"
                     /locus_tag="RPM73_002970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDX6521308.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2613..3155
                     /gene="infC"
                     /locus_tag="RPM73_002971"
     CDS             2613..3155
                     /gene="infC"
                     /locus_tag="RPM73_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDX6521309.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3252..3449
                     /gene="rpmI"
                     /locus_tag="RPM73_002972"
     CDS             3252..3449
                     /gene="rpmI"
                     /locus_tag="RPM73_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDX6521310.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3502..3858
                     /gene="rplT"
                     /locus_tag="RPM73_002973"
     CDS             3502..3858
                     /gene="rplT"
                     /locus_tag="RPM73_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDX6521311.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3975..4099
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3981..4025
                     /gene="pheM"
                     /locus_tag="RPM73_002974"
     CDS             3981..4025
                     /gene="pheM"
                     /locus_tag="RPM73_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDX6521312.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4308..5291
                     /gene="pheS"
                     /locus_tag="RPM73_002975"
     CDS             4308..5291
                     /gene="pheS"
                     /locus_tag="RPM73_002975"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDX6521313.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5306..7693
                     /gene="pheT"
                     /locus_tag="RPM73_002976"
     CDS             5306..7693
                     /gene="pheT"
                     /locus_tag="RPM73_002976"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDX6521314.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7698..7997
                     /gene="ihfA"
                     /locus_tag="RPM73_002977"
     CDS             7698..7997
                     /gene="ihfA"
                     /locus_tag="RPM73_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDX6521315.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8098..9078
                     /gene="btuC"
                     /locus_tag="RPM73_002978"
     CDS             8098..9078
                     /gene="btuC"
                     /locus_tag="RPM73_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDX6521316.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9141..9692
                     /gene="btuE"
                     /locus_tag="RPM73_002979"
     CDS             9141..9692
                     /gene="btuE"
                     /locus_tag="RPM73_002979"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDX6521317.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9692..10441
                     /gene="btuD"
                     /locus_tag="RPM73_002980"
     CDS             9692..10441
                     /gene="btuD"
                     /locus_tag="RPM73_002980"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDX6521318.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISII"
     gene            10519..10983
                     /locus_tag="RPM73_002981"
     CDS             10519..10983
                     /locus_tag="RPM73_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6521319.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11230..11943
                     /gene="ydiV"
                     /locus_tag="RPM73_002982"
     CDS             11230..11943
                     /gene="ydiV"
                     /locus_tag="RPM73_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDX6521320.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12006..13442
                     /locus_tag="RPM73_002983"
     CDS             12006..13442
                     /locus_tag="RPM73_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDX6521321.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWSLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(13446..13565)
                     /gene="hemP"
                     /locus_tag="RPM73_002984"
     CDS             complement(13446..13565)
                     /gene="hemP"
                     /locus_tag="RPM73_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDX6521322.1"
                     /translation="MRRISSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
BASE COUNT         3396 a         3176 c         3643 g         3390 t
ORIGIN      
        1 acagcatcga acttaagtca gaggtatcaa gtagggtgat gagctggcgc gcaaatttat
       61 taataggctc tgatgaaggt gttggtatgt cgctatcgat gtgcaatcta tgaggagact
      121 tataatgagt tctgataatg atatcgtttt gactcataat ctattcgcct tatatatatc
      181 tggaggtttt gaggatgatg attaattatt atcgccagaa cgaaatgtgc acgaaaaatt
      241 gttataagaa ttgtgtcact tttatcattg tgatatgacc acttcgtcat taatctcatg
      301 agcgtttaaa atactaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg
      361 ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg atgttatcag tggttcttta
      421 agcgttttgc tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa
      481 aggtacaatc tccctttttt tagtgttaac atcgctcaac cgggtggaag gtgagaggtt
      541 aatgtctttc atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt
      601 tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc
      661 actgcaaata aggatataaa atgcctgtta taactcttcc tgatggcagc caacgccatt
      721 acgaccacgc tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag
      781 cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg
      841 acgcacaact gtccatcatt accgccaaag acgaagacgg tctggagatc attcgtcact
      901 cctgtgcgca cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa
      961 tcggcccggt tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc
     1021 aggaagatgt cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg
     1081 tcattaagaa gaaagtcagc tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga
     1141 gctacaaagt ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact
     1201 tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc
     1261 atcatttcaa actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa
     1321 tgttgcaacg tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc
     1381 agcgcctgga agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt
     1441 accatatgca ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct
     1501 tccgtgaact ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta
     1561 aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca
     1621 aagatgcaat gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact
     1681 gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc
     1741 gtatggccga gtttggtagc tgccaccgta atgagccgtc aggttcgctg catggcctga
     1801 tgcgcgtgcg tggatttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc
     1861 gcgatgaagt taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg
     1921 agaagatcgt cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt
     1981 gggatcgtgc tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat
     2041 atcaactggg tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc
     2101 tcgatcgtgc atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga
     2161 gcgcctccta tgtgggcgaa gacaacgagc gtaaggtacc ggtaatgatt caccgcgcaa
     2221 ttcttgggtc gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc
     2281 cgacctggct tgcgccggtt caggttgtta tcatgaatat taccgattca cagtctgatt
     2341 acgttaacga attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga
     2401 gaaatgagaa gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc
     2461 tggtctgtgg tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta
     2521 aagacctggg aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca
     2581 gccgcagtct taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg
     2641 cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg
     2701 aaggcgagca gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg
     2761 gagtagactt agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt
     2821 acggcaaatt cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaaaag
     2881 ttatccaggt taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa
     2941 aactccgcag cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt
     3001 tccgcggtcg tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag
     3061 acgatttgca agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga
     3121 tgatcatggt gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg
     3181 agccttcggg cttcacaggc tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg
     3241 aagttattaa aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa
     3301 ccggtaaagg tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag
     3361 cgaccaaacg taaacgtcac ctgcgtccaa aagccatggt ttccaaaggc gatctgggcc
     3421 tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa
     3481 tatagataca ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt
     3541 cacaagaaaa ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc
     3601 gttgccttcc aggctgttat caaagctggt cagtatgctt accgtgaccg tcgtcaacgt
     3661 aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaacggt
     3721 atttcttaca gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag
     3781 atcctggctg atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg
     3841 aaagcagctc tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa
     3901 cgccatcaaa acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt
     3961 accaaaaggt aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca
     4021 cctgaatcca ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc
     4081 cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa aagccagtct atcaggcgta
     4141 attgtcgcag ccagtctgga cacggccagc gcggagaaac cggagcgtat ttaagtacgt
     4201 gagaatttcg agcactgccc gggaccaaaa tggcaagtaa aatagcctga tgggataggc
     4261 tctaagtcca acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg
     4321 cagaactggt tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag
     4381 ataatgtgcg cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc
     4441 tgcgtgagct gccgccagaa gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag
     4501 agcaggttca gcaggcgctg aatgcgcgta aagcggaact ggaaagcgca gcgctgaatg
     4561 cgcgtctggc ggcggaaacg attgatgtct ccctgccagg tcgtcgcatt gaaaacggcg
     4621 gtctgcatcc ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct
     4681 ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca
     4741 ttcctggtca ccacccggcg cgcgctgacc acgacacttt ctggtttgac actacccgcc
     4801 tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga
     4861 ttcgtatcat cgcgcctggc cgcgtttatc gtaacgacta cgaccagact cacacgccga
     4921 tgttccatca gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag
     4981 gcactctgca cgatttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc
     5041 cttcctactt cccgttcacc gagccttctg cagaagttga cgtcatgggt aaaaacggta
     5101 aatggctgga agtgctgggt tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca
     5161 tcgacccgga agtttactct ggtttcgcct tcgggatggg gatggagcgt ctgactatgt
     5221 tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa tgatctgcgt ttcctcaaac
     5281 agtttaaata aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt
     5341 gaacccggcg attgatagcg atgcgctggc gaatcaaatc actatggcgg gcctggaagt
     5401 tgacggtgta gaaccggttg ccggtagctt ccacggcgtg gtcgttggtg aagtggttga
     5461 gtgtgcgcag catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg ttggcggcga
     5521 tcgcctgctg gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt
     5581 agctaccatt ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg
     5641 tgaaccgtct gaagggatgc tgtgctcctt ttctgagctg ggaatttctg acgatcatag
     5701 cggcattatc gagctgcctg cggatgcgcc gattggcacc gacatccgtg aatacctgaa
     5761 acttgatgac aacaccatcg aaatcagcgt gacgccaaac cgtgccgact gtttaggtat
     5821 cattggtgtt gcgcgtgacg ttgccgtgct gaatcagctg ccgctggttg aaccggaaat
     5881 cgttccggtt ggtgcgacca tcgacgacac gctgccgatt acagtcgaag cgccggaagc
     5941 ctgcccgcgt tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct
     6001 gtggatgaaa gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt
     6061 caccaactat gtactgctcg aactgggcca gccgatgcac gctttcgata aagatcgcat
     6121 tgaaggcggc attgtggtgc ggatggcgaa agagggcgaa acgttggtac tgcttgatgg
     6181 cactgaagcg aagctgaatg ctgacactct ggtcatcgcc gaccacaaca aggcgctggc
     6241 gatgggcggc atctttggtg gcgaacactc tggcgtgaat gacgaaacgc aaaacgtctt
     6301 gctggaatgc gctttcttca gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct
     6361 gcatactgat gcgtctcacc gttatgagcg tggcgttgat ccggcactgc agcacaaagc
     6421 gatggaacgt gcgacccgtc tgctgatcga catctgcggt ggtgaggctg gtccggtaat
     6481 tgatatcacc aacgaagcaa cgctgccgaa gcgtgcaacc atcactttac gtcgtagcaa
     6541 actggatcgc ctgatcggcc atcatattgc ggatgagcag gtaactgaca ttctgcgtcg
     6601 tctcggctgc gaagtgaccg aaggcaaaga cgagtggcag gcagttgcgc cgagctggcg
     6661 tttcgatatg gagattgaag aagatctggt cgaagaagtc gcgcgtgttt acggctacaa
     6721 caacatcccg gatgagccgg tacaggcaag cctgattatg ggtactcacc gtgaagctga
     6781 cctgtcgctc aagcgcgtga aaacgctgct caacgacaaa ggctatcagg aagtgatcac
     6841 ctacagcttc gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact
     6901 gctgccaagc ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct
     6961 gctggcaacc gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag
     7021 cggtctgcgt ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt
     7081 agccggtgtg atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt
     7141 tgatttctat gatttgaaag gcgatcttga atccgttctc gacctgaccg gtaaactgaa
     7201 tgaggttgag ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat
     7261 ttatctgaaa ggtgaacgta ttggttttgt tggggttgtt catcctgaac tggaacgtaa
     7321 actggatctt aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg
     7381 cgtggtgcct caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt
     7441 ggtggtcgca gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt
     7501 aaatcaggta gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg
     7561 gtataagagc ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga
     7621 ggagattgcc gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc
     7681 attgagggat tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata
     7741 agcttgggct tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc
     7801 gtcgcgctct ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc
     7861 gtgataagaa tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag
     7921 cacggcgcgt ggtgaccttc agacccgggc agaagttaaa aagccgggtc gaaaacgctt
     7981 cgcccaaaga cgagtaatct gatctaacta aaaaggccgc tctgcggcct tttttctttt
     8041 cactgtcgaa gagtcaccgt aaaatcaacg ccatgacact tcagcagaac ggataccatg
     8101 ctgacacttg cccgccaaca acagcgacaa aatattcgct ggttattatg cctgtcagtt
     8161 ttgatgctgc tggcgcttct cttaagcctt tgcgccggtg aacaatggat ctcgccaggt
     8221 gactggtttt ctcctcgtgg cgaactgttc gtctggcaga ttcgcctgcc acgtacgctg
     8281 gctgtattgc tggttggtgc ggcgctggct atatccggcg cagtaatgca ggcgttgttt
     8341 gaaaatcctc tggcagaacc tggactactt ggcgtctcta acggcgcagg cgtagggctt
     8401 atcgccgcgg tattgcttgg gcaagggcaa ctcccaaact gggcactagg gctgtgtgcg
     8461 attgctggcg cgcttatcat cactttaata ctcttacgtt tcgcccgtcg tcatctttcg
     8521 accagtcggt tattgctggc tggcgttgca ttagggatta tctgtagcgc actaatgacg
     8581 tgggctatct acttttccac ctctgttgat ttacgtcagc tgatgtactg gatgatgggc
     8641 ggttttggcg gcgtagactg gcggcaaagc tggctgatgc tggcattgat ccccgtgttg
     8701 ttgtggatct gttgtcagtc caggccgatg aatatgttag cacttggcga gatctcggcg
     8761 cggcaactgg gtttacccct gtggttctgg cgcaatgtgc tggtggcagc gaccggctgg
     8821 atggttggcg tcagtgtggc gctggcgggt gctatcggct ttattggtct ggtgatccca
     8881 catattcttc ggttgtgtgg tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg
     8941 gcaggggcga gcgcattgct gctggccgat gttgtagcgc gcttggcatt agctgccgca
     9001 gagctgccta ttggcgtggt caccgcaacg ttgggtgcgc cggtgtttat ctggttattg
     9061 ttaaaagcag gacgttagcc gcaaaaagac ggtctatgat taaaagctaa gatttttact
     9121 gaccacaccc aggagaaacg atgcaagatt ccattctgac gaccgtagtg aaagatatcg
     9181 acggtgaagt gaccacgctg gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg
     9241 cctcaaagtg tggcttaacg ccgcaatatg agcagttgga gaatattcag aaagcctggg
     9301 ccgatcgagg ttttgtggtg ctgggattcc cgtgcaacca gtttctggaa caagaaccgg
     9361 gcagcgatga agagattaaa acttactgta ccaccacatg gggggtgacg ttcccgatgt
     9421 tcagtaagat tgaagttaat ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg
     9481 cagcgccgac cgcagtcgcg ccggaagaga gcggattcta tgcccgtatg gtcagcaaag
     9541 gtcgtgcacc gctgtaccct gatgatattt tatggaattt tgagaaattt ttagttggca
     9601 gggacggatt ggtcatccag cgtttttccc cggatatgac gccggaagac cccattgtga
     9661 tggaaagcat taaactggcg ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt
     9721 tgcggaatct acccgcctgg ggccactttc tggcgaggtt cgggctgggg agatcctgca
     9781 cctggtgggg ccgaatggcg cgggtaagag taccttgctt gcacgaatgg ccggaatgac
     9841 cagcggtaag ggaagcattc agttcgcggg gcaacccctg gaagcatggt ccgcaacaaa
     9901 actcgcgctg catcgcgcct atctttcaca acagcagacg ccgccgtttg caatgccggt
     9961 ctggcactac ctgacactgc atcagcacga taaaacgcgt accgaactac tgaatgatgt
    10021 cgcaggggcg ctggctcttg atgacaaact cggacgtagc accaatcaac tttccggcgg
    10081 tgaatggcaa cgcgtacgtc ttgctgcggt ggtgctgcaa atcacaccac aagccaatcc
    10141 cgcaggtcaa ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag
    10201 tgcgttagac aaaattctga gcgctctgtg tcagcaagga ctggcgattg tgatgagcag
    10261 tcacgatctc aaccacacat tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa
    10321 aatgctggcc agtggacgca gagaagaggt gctcacgccg ccaaatctgg cgcaggccta
    10381 tgggatgaat tttcgccgtc tggatatcga aggtcacaga atgctgattt cgatcatctg
    10441 atttcattag tatcttgaaa atcgggtaat tacgacgcta aattaatgca agaataaaaa
    10501 acagaggaac cgtacggaat gcgtttctgc cttattttga tcacagcact gtttctggcc
    10561 gggtgtagcc accataaagc accgccgcca aatgccagac tttctgattc gattaccgtt
    10621 attgccggtt tgaacgacca gctacaaagc tggcatggca cgccgtatcg ttatggtggc
    10681 atgacgcggc gcggtgtgga ctgttcggga tttgtggttg taacgatgcg cgatcgtttc
    10741 gatttgcagc tgccccgaga aaccaaagaa caagcctcta tcggcacgca aattgataaa
    10801 gacgagttgc tgcctggtga cctggtcttt ttcaaaacgg ggtccggaca aaatggtttg
    10861 catgtaggta tttacgatac caacaaccaa tttatccacg cctctaccag caagggagtg
    10921 atgcgttcct cacttgataa tgtctattgg cagaaaaatt tctggcaggc gagacgaatc
    10981 tagtatgtcg aaaaatggac ggcgatgata catcgccgtc catttactcc tgaatacagt
    11041 agtattttgc agacaaaaat tccgtaaatg gattattcgt ttataatcag gataatctaa
    11101 aaaaaggacc cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat
    11161 tgttatgtct tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg
    11221 acggttacca tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg
    11281 ataagagata accagcaact ccttgtcggc gttgagttaa ttacccattt ctccagtgaa
    11341 gatggcacgg tgcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat
    11401 tggcaattat tttcagagca gttggaatta ctaaaatcat gccaacattt ttttattcaa
    11461 cataaactct ttgcctggct taatttaacg ccacaagtgg ctacattatt attagagagg
    11521 gataattacg caggcgaact attaaaatat ccgtttattg aactgttgat taatgagaat
    11581 tacccgcatc tcaatgaagg aaaagataat cgcggtcttt tatccttatc gcaagtgtat
    11641 ccccttgtgt taggtaattt gggagcaggc aatagcacaa tgaaagcggt ttttgatggt
    11701 cttttcaccc gcgtcatgct ggataaaagt tttattcagc aacaaatcac gcaccgctcc
    11761 ttcgaaccgt ttattcgtgc gatccagtcg caaatttccc cttgttgcaa ttgcatcatt
    11821 gcgggaggca ttgatacggc agaaatattg gcacagatta cccccttcga tttccatgct
    11881 ctccagggat gcctgtggcc tgccgttccc ataaatcaga taacgacgtt ggttcagcga
    11941 taacccttct gtttgctggt gtttaagacg agagtaaccg tctacactat caaacaggag
    12001 gatctatgac cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag
    12061 cactttcccc tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta
    12121 acacgctgag tattccatcg tcgctgttta aaaatgctgc aggtgtctgg ggcggcgaaa
    12181 ccctactgcc tggtatgtca ccactggcgc aggtttacag tggtcatcag ttcggcgtct
    12241 gggcgggcca actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg
    12301 gcactacaat ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg
    12361 atggacgggc ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt
    12421 atctgggcat tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc
    12481 gggaaacggt ggagccaggc gcgatgctga tacgtgtggc accaagtcat ctgcgctttg
    12541 gtcatttcga acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttagctgact
    12601 ttgccattcg tcattactgg tcacatcttg aagatgatga ggacaaatac cgtctctggt
    12661 ttaacgatgt tgtcgcacgt accgcatcgt taattgccca atggcagacg gtcggctttg
    12721 ctcatggggt gatgaatacc gacaacatgt cgctgctggg gctgacgctt gattacgggc
    12781 cgtttggttt tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag
    12841 ggcgttacag ctttgataat caacctgccg tcgcgttgtg gagtttacag cgtctggcgc
    12901 agacattgtc accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc
    12961 aggttttgtt gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc
    13021 aaaaagagga taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg
    13081 attatacccg cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc
    13141 tacgtgatga gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc
    13201 gtttgcaaca agacgaggtt agcgatagtg agcgtcagca gctgatgcaa agcgttaacc
    13261 cagctctggt gttgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg
    13321 atatgacgga attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagctg
    13381 atgactatgt cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt
    13441 aaaggctact tggtcaacag cagcttgcca gcctgagttt tacggagcag atattcttgc
    13501 ccgtcatgat cgataatcag tttgccatcc ggacctaaca gtgtctggct ggaaattcgc
    13561 cgaataggct gcggggaagc ggtcttgtga ttggcatccg tttca
//
LOCUS       DAPPNJ010000122        13401 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000122 DAPPNJ010000000
VERSION     DAPPNJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.123"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            89..919
                     /locus_tag="RPM73_002985"
     CDS             89..919
                     /locus_tag="RPM73_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDX6521323.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1060..1833)
                     /gene="uxuR"
                     /locus_tag="RPM73_002986"
     CDS             complement(1060..1833)
                     /gene="uxuR"
                     /locus_tag="RPM73_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDX6521324.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2048..3508)
                     /locus_tag="RPM73_002987"
     CDS             complement(2048..3508)
                     /locus_tag="RPM73_002987"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDX6521325.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3589..4773)
                     /gene="uxuA"
                     /locus_tag="RPM73_002988"
     CDS             complement(3589..4773)
                     /gene="uxuA"
                     /locus_tag="RPM73_002988"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDX6521326.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5113..6456
                     /gene="gntP"
                     /locus_tag="RPM73_002989"
     CDS             5113..6456
                     /gene="gntP"
                     /locus_tag="RPM73_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDX6521327.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6700..7602)
                     /gene="fimH"
                     /locus_tag="RPM73_002990"
     CDS             complement(6700..7602)
                     /gene="fimH"
                     /locus_tag="RPM73_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDX6521328.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7622..8125)
                     /gene="fimG"
                     /locus_tag="RPM73_002991"
     CDS             complement(7622..8125)
                     /gene="fimG"
                     /locus_tag="RPM73_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDX6521329.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNNGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8138..8668)
                     /gene="fimF"
                     /locus_tag="RPM73_002992"
     CDS             complement(8138..8668)
                     /gene="fimF"
                     /locus_tag="RPM73_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDX6521330.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8678..11314)
                     /gene="fimD"
                     /locus_tag="RPM73_002993"
     CDS             complement(8678..11314)
                     /gene="fimD"
                     /locus_tag="RPM73_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDX6521331.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11381..12106)
                     /gene="fimC"
                     /locus_tag="RPM73_002994"
     CDS             complement(11381..12106)
                     /gene="fimC"
                     /locus_tag="RPM73_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDX6521332.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12143..12682)
                     /locus_tag="RPM73_002995"
     CDS             complement(12143..12682)
                     /locus_tag="RPM73_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6521333.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12747..13295)
                     /gene="fimA"
                     /locus_tag="RPM73_002996"
     CDS             complement(12747..13295)
                     /gene="fimA"
                     /locus_tag="RPM73_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX6521334.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3242 a         3475 c         3248 g         3436 t
ORIGIN      
        1 catgtcattt gtttacttct ctttttacta cagaaaaatt tatctatttt tctaaacatc
       61 agtgaaatac tcctaaaagg taactattat gtctatgcca ttaagcaatg cccttcagag
      121 tcaaattatt acagataatc acttccttca tcacccaaaa gtcgacaatg agttaacacg
      181 taaatatgaa cgagctcgcc tggatacgga aaatatctat cttctgcctc ttgctcgtgg
      241 caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaacttg atattagtaa
      301 agagtcctgg ccatttaatt atgtgacaga agcatgcaga gaatccgatg gtattacaac
      361 aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg aaatatatgg
      421 tggtatatgt aaaaaggcgc atgctgctac agaattagca gaaggtttac ggctaaattt
      481 atttatgaag agccctttcg atcctgttga ggactataca gttcatgaaa taacattagg
      541 ccctggttgc aatgttccag gttacgccgg aaccacgata ggatatattt caacccttcc
      601 agtttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct atattgatca
      661 gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg cattacttaa
      721 tgctaatatt ggaatgggaa atgacccaat aattggtata gaagcctatc ccggtaccgc
      781 ggagatccat gcaaaaatgg gatataacgt tatccccggt gatgaagatg cgccactcaa
      841 acgcatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa atggtgaatg
      901 gaattatatt ggtaaatagc cactgagaag aatgagtact aatattcagg ttatacagat
      961 atttaaacaa taccctgcaa tttacaaagt aatactcatt gcgacttctt aatttaagaa
     1021 agcgcaagga acgtttaccc ttgcgcttat tataataagt caggcgtcga ctttatccag
     1081 cggccatgaa tcaaacagat agccgtcaaa ataaatatcg tcaacgttcg agaattccag
     1141 cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg ctcgggcatc
     1201 ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt tgcgatacag
     1261 gctgtcatcc agatggctgt gcaactggat ccacattggg ttgttttccc gccactgcca
     1321 ggactgacgg aacagctcca ccagcatgct gttatgcgtt gcttcggcaa tagcgagatg
     1381 gaactgcatg tcaccgcttt cgctgctgcc cggcgcactg gaagccagtt cacgctcttc
     1441 cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca gggcggcaaa
     1501 ctcggcgatg ttactttcca gtaactgacg cgcctgtaac agctcaaaag gaccggcatc
     1561 gttgcagacg ttggcatccg gactgtctgt gttgtggctg cctgagttat caagaacata
     1621 gataccggca ccccggcgta cttccaccag ccctttgatc tccagcatga tcagcgcttc
     1681 acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg gcggcagccg
     1741 ttcgccaggg ttgtacggcg tctttatgat cagatcgcgg atcatcgccc cgacttcctg
     1801 gtaaggtctt tgcgcagagg tggcagattt catggtgttg tgtccgttag gtgcggttaa
     1861 tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg caattttacg
     1921 gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac aggtgggcaa
     1981 aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct gtagggtaat
     2041 cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg
     2101 ccgccgtcac tgcgccaaca aagtcggcat tctgcggcag atcatcggca aaaataccgc
     2161 tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgttg atcttctgga
     2221 actccgccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc acgccctggg
     2281 tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg ccagctgccg ccgttttgca
     2341 ggtgcagacg caccgggtcc agcagacgct gcggtaactt ctggctgccg tccatcgcaa
     2401 tctgccaggt acggtgacgc agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat
     2461 aggcgttcag gtctgtacct tccggcatcg acagcgttgg cgcttgttcc tgcatcatca
     2521 gggcaaaggc cgctttgcga taagccgggt tagtcatggt gtcggcaatg gtttcatagc
     2581 cgccgaggta accgaggtac gccagaaaag agtggctgcc gttcagcata cgcagcttca
     2641 tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc cactttatcc cagtccgggc
     2701 gaccattaac gaagttatct tcaatcaccc actgacggaa cggttcgcag gcaatggcgc
     2761 acgggtcgta aacacccagc tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg
     2821 gaacgatgcg gtcaaccatg gtgcacggga aggtgacatt ttcttcaatc catgccgcca
     2881 gctgcgggtc acgcgcctga gccagcccca gtaccgcgac cttcgccaca tgtccgtttt
     2941 cacgcacgtt atcgcaggac atcaccgtaa acgctttcaa ccctttttca cgacgcagac
     3001 gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt
     3061 gcttgatcag cgggttattg agatccagtt gaccgcttgc cgcatcagcg cagtagcctt
     3121 tttccgtgac cgttagagag acaatcgccg tttgcggacg cgccatcgcg ttgagaatac
     3181 cttcgcagcc atcgatttcc ggatgcagcg cttctttcat cgaaccgata attttcagct
     3241 cggtgctctc tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt ttcaggtttt
     3301 cgatcagcac gcggtcgttg cctggcatca ggttaacttc gcagatgccc cagtcgctgt
     3361 cggtgctttc cagcagatga tgggtataca gcgcctggtg cgcgcggtga aacgccccgc
     3421 aaccgagatg cacaatgcgt gattccagac gagaatgatc ccatgacggg cgggcaaccg
     3481 gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt cagagagtga
     3541 cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtggatatt aacggctaaa
     3601 gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt tcagacgacc
     3661 aattgcggag taacctgggt tggttttctt cttcaggtcg tccagcatct gatgaccgtg
     3721 gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt gttcttcttc
     3781 aacaatcgct ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg
     3841 gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg
     3901 accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt aggaaccggt
     3961 gcacatggta aaaccgtttg ccatgctgtt tacggtatca accatccact gcatatcttc
     4021 aatggtggaa acaatgcgcg gcaggccgag gatcgggcgc ggcggatcgt ccgggtgaac
     4081 agccatacgc acgccgactt cttcagcaac tggaataatc gctttcagga agacggcaaa
     4141 gttttcgcgc agcttcgctt tgtcgatatc tttgtacagc tccaggtgtt tacggaactg
     4201 gtcgagggtg tagccttctt ccgcgcccgg aagaccagca atgatgttac gggtcagacg
     4261 cgctttgtct tcgtcgctca tagtggcgaa gcgaacagca gcctgagcaa tttcttcttc
     4321 ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg cagcgaattc
     4381 gatctggtcg aagcgcagcg ctttggagcc gtctggcagc acgtattcga ggtcagtacg
     4441 ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccgc actgcgccag
     4501 gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag tgtgggtttt
     4561 gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg cgtcttcaac
     4621 gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg ggatatggtg
     4681 cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag aaaccggatc
     4741 gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac atagtgggtt
     4801 ccacagcaaa gagacgcgga accctggagt tgtttaggta tccggatgaa caaccagacc
     4861 gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg gtaagacatg
     4921 tcaacccgaa ttctgaaatt ggttaaccac atcacaagaa tttcactctc atggttgttg
     4981 ctgcaaattg cagcaaaggg taatcgatgg ttattgatca gaggatgtga cattcatcgc
     5041 aacaatggtt gaccaattta cataacatgt cgcccaaaat aacaacggtt caaccaggta
     5101 aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg tattcggcat tggcctgatg
     5161 ctggtactga atttgaagtt caaaatcaat tcaatggtgg ctttgttggt ggcggcgctg
     5221 tccgtcggga tgctggcggg catggatttg atgtcgctgc tgcacactat gaaagcgggc
     5281 ttcggcaaca cgctggggga actggctatc atcgtggtgt tcggtgcggt catcggtaaa
     5341 ttgatggtcg actccggcgc ggctcaccag atagcgcata cgctgctggc gcgtctcggt
     5401 ctgcgctatg tacagctgtc ggtgattatc atcggcctga tttttggtct ggcgatgttc
     5461 tatgaagtgg cctttatcat gttagcgccg ctggttattg ttattgccgc cgaagctaaa
     5521 attccgttcc tgaaactggc gatcccggca gtagcagctg ccactaccgc acattcactg
     5581 ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga tatggggatg
     5641 gtttatatct atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg tctgatcctg
     5701 ccgaagttcc tcggcaatct tgagcgccca acgccatcat tcctgaaagc agatcaaccg
     5761 gtagatatga ataatctgcc ctctttcggc gtttcgattc tggtgccgct gatcccggcg
     5821 atcattatga tctctaccac catcgccaat atctggctgg taaaagatac ccctgcctgg
     5881 gaagtggtta actttatcgg ttcctcgccg attgcaatgt ttattgcgat ggtggttgca
     5941 ttcgtactct ttggcaccgc acgtggtcat gacatgcagt gggtgatgaa cgcttttgaa
     6001 agcgcggtga agagcattgc aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag
     6061 cagaccatca tcgacaccgg cattggcgac accatcggca tgttgatgtc ccacggcaat
     6121 atctcgccct acatcatggc atggctgatc actgtgctaa ttcgtctggc gacgggtcag
     6181 ggtgtcgttt cggcgatgac cgccgccggg attatcagtg ctgcaatcct tgatccagca
     6241 actggtcagc tggttggcgt gaatccggcg ctgctggtac tggcgacggc tgcgggttcc
     6301 aacaccctca cccacattaa cgatgcatct ttctggttgt tcaaaggtta ctttgacctg
     6361 tcagtaaaag acacgttgaa aacctggggt ctactggagc tggtcaactc cgtggttggg
     6421 cttattattg tgctgattat tagcatggta gcgtaaaagg acaccagagc ctgccaatgg
     6481 caggctcaga ctgatgacaa agtctaaaaa tgtgcccaga cagtcccctc gcccctccgg
     6541 ggtgagggtt agggtgaggg gaaaagaccc gcaaagtgcg ctttgtttat gccagatgcg
     6601 acgctgacgc gtcttatctg gcctacaaag ggctaacgtg caggttttta gcttcaggta
     6661 atatttgcgt acctgcatta gcaatgccct gtgatttctt tattgataaa caaaagtcac
     6721 gccaataatc gattgcacat tccctgcagt cacctgccct ccggtacgag cgtaatttgc
     6781 cgttaatccc agacttaccg ccgaagtccc tactgctcct aacgataccg tgttattcgc
     6841 tggaataatc gtaccgttgc gcgtcaactg tacgccgacg ccctgcgctg gtgaaaacga
     6901 ggcggtattg gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga ggtaatatcc
     6961 caggttttgg cttttcgcac aataaacggt aagaggaatt ggcactgaac cagggtagtc
     7021 cggcagagta acggtgacat cacgagcaga aacatcacag ccgccagtag gcaccaccac
     7081 atcattattg gcgtaaatat tccacacaaa ctggaaatca tcgctgttat agttgttggt
     7141 ctgtcgcaaa ataagcacgg caattaatga gccagcttta atcgccaccc cgcccgcact
     7201 gctcacaggc gtcaaataaa gcgccaccgg ccacggctta tccgttctcg aattataaac
     7261 aacgcgcggc gtttcgctgg tggtcggaaa tggatagcta ctgccactat attttacggt
     7321 cccggaaaaa ttagataaca cgccgccata agccgagcct cgttgcagtg tgacatagtc
     7381 tgtaatggtt tccggataat cgttatggca aaagatttgc gtcgaaagat ctacgaccag
     7441 gttttgcccc acattcacgg caggcgcaag gtttacataa acattagcgc tgccaccgcc
     7501 aatagggata gcggtaccat tggcggtttt acaggcgaat gaccaggcat ttaccgacca
     7561 gcccatcagc agtacagcaa acagggtaat aactcgtttc attacaatca tctcttcggg
     7621 ttcagctgta ggtataggtg atgctaatca ctgcctgaat ggttccctga gtggctccgc
     7681 catttactgt caatgctctg acctgtaacg ggaagtgcgc tgattgtgag gaatcatcca
     7741 cctgaactgt tttggttgcg ccattattca atgtgttgcc actgtcatcc tgtagctcta
     7801 actggatgtt ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg gctgccccgc
     7861 tgaagctggc agtgaccctc gacgttccca ccggacaatt agtcaactca agcgcaacat
     7921 catgccaggc cgatgccgcc ccggcagaca taagactgaa agaataaaga tcgccgagat
     7981 caaccgtggc attggtggtg gaaactgtgc acggtttggc gacgacctta ccgttcaccg
     8041 tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag cgccaatatt gccgccaata
     8101 aatacccacg tttgcgccat ttcatgagca tctccagtta ctgatattca agagtgaagg
     8161 tagccgtggc attgatatgc cccgcagtga ctggcacctg tgtcgccatt agccgggcgt
     8221 aaaaattcag cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc gcggacgatg
     8281 gagcattaag gggtatttga ttttgctgct cattcagaag ctgtattccc agtcccgcag
     8341 ccgctgacac cgtattttca agtgcaagca ggttggcatt gtggctatct gcaacgccag
     8401 taaacccaac ctttacggca gaaacggcgt taccacaggg tgacagcaaa atacgaaatg
     8461 gaacgacagg agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat
     8521 caacagtaaa attggttgat tcagcggcca cactacagcc gttatctctg acatagccgc
     8581 ggatagtaat cgtgctatcc gcagccaaag cgtgactcac cgccagccac aaaaaagcgc
     8641 acagaagata aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct
     8701 gggttaataa ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat
     8761 tttcctcttc tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa
     8821 cctgaccatt atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc
     8881 caaacggcag cggcttatta ttgtgggtta gcgtcatgag cagttttatc ccaacgcgcg
     8941 ctttaaactc tgctcgcacg atcgccccac gagtgggaac aacgttagcg accgcgttat
     9001 ctaaatcgac gttatcagcc agggtattgg tatccagcgc cactctgttt tcccgatatt
     9061 cagtggcata aggcagcacg gcataaccac gccagtcggt acgcaccccc gtctggtttt
     9121 cgacttttgc atcttttgcg ccaggcgctt taacaagcac cactgtttcg tttaacggct
     9181 gccccagcgt tacgccattg gcatgagcca ataccccacc gctgactccg taatagagct
     9241 gcttaatatc atcgctatgg ctgtaaccga tattggcatt gccgtaacca ccgcgataat
     9301 tcagcgtggc gtagcctgtg cttccgctat taccatcgcc tcccccggca tagccggttt
     9361 gcacgctata gctgaggttg ttgtcttcca gcaacgtacc gtatacgcca gccagattgg
     9421 tcatccgacc gttgagatcg tgtgacatgc tgtagctggc actggcatgt cgccactgag
     9481 atttactgtc agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct
     9541 gatcccgtcc tttttgccag gcatttttcg tcaggctata gctgagcgtc cagttgatat
     9601 cttcgaatgc agtatttaaa ccagcctgga attgctcatc gacattactc gttccccaat
     9661 aagtttgatg gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg
     9721 tgagctgtaa tttcccgcgt ttgttataag cgaggttgta atagtcggtg aatttcggct
     9781 taacctgaat aactccgtcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg
     9841 tatcagcgaa attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg
     9901 tgcctgactc attgagcgat ttgttataga gaaaacgcac cgattgtccg tcatgctgac
     9961 tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgccc
    10021 ccatgttttt cccgataccg aaattaaaag cacgataacg atccgccagt tgcgttccac
    10081 catatattgt ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcggggtt
    10141 tttcctgctg cgcatttcca ctacggtatt ctcctgccgt aatggaataa cgagtatgcc
    10201 cttcacgttg caaaagcggg actgacgaat agggtacggt aaaaatctgc gtgctgccgt
    10261 cagcctcttt gatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga
    10321 tggtaaaagg ccccggtggc accgtactat tataaatgtc atacccattt tgtttaatag
    10381 tgacctgtgc agtaccacga gcaataccgt ggatcaccgg ggcaaatcct ctttggctat
    10441 cgggtaacat attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa
    10501 tatcgccctg agtataacca tcacccagcg tcagccggga acgtaacggt attatgtctc
    10561 gctcaagcca ggtattgata tgctgccatt tatttttgct acctgatgat ctgtcgctac
    10621 tgttataact ccaggtggta ttgtcgcgta aacgccacgc accaatattt aacccactct
    10681 gtaggtttaa atatgcataa tggctgttac ccccaatccg attctgtaca ctatttccgc
    10741 tgaaattata attgagcaat ccggcattaa taccgggatc ccataactca ggaggaatat
    10801 aaccacgcgc gcgattactc ataaatgcct gagggatcgt caggttgagt cgctgctgac
    10861 caacatctaa atgcgcagta gcgtcatgga tcattgaggt taatggcacg caggcatcat
    10921 ccgccagcag attcataccg gagacagaag ccgtattcag ccccatactg gcaagttgcg
    10981 cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc cgtattaaat gtgacatcac
    11041 gcgttgccat ataaccatta ttcaaataga tatcgacacg atacgtccct ggcggtaatt
    11101 cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc gctaaaaagc
    11161 gcggattaaa atagagttcg gcagatgaca aaggtgcctg tgcggcaaaa gcacaggcga
    11221 caacgagccg gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag cattgtgtgt
    11281 ttcgctggta aagtcttaaa ttcagatatg acattattcc tccggtttaa aatcacccgg
    11341 cagtcaattc tttttattca ggcgaaaaat tccccctgcg ttattccatt acgcccgtca
    11401 ttttgggggt aagtgcgcca taatcattta ttgttcggta agtaatattg cttcctgcat
    11461 cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac caatgcattt tcaagaaccc
    11521 gggttccggc attcaactct gttaccgtca ggtaataggg tgtcgggtta atcagcgtca
    11581 gggaattcgc gctacgacga aatcttaatt tttctgcggc ctgatcgggt ggcaacgcta
    11641 atttagccgg acgatagtac agtttaatgc ggctgataat tgcgagctgt agcgtattct
    11701 cagtcaattt tgatttatcc attgacggaa tcgctttaac attcatccag aataaacttt
    11761 cccgatcctg tggcaattgg ttattagttg catcaagaat acgcaaggta ttctctcttt
    11821 ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg accatccttt acaccatcgg
    11881 cattttccac ccatgattga attaaatagg tactattttc atcattattt gtcacggcaa
    11941 gttgcacttg tttttgccct gccggataaa ttacgcgagt cgcacctaag gccactcccg
    12001 cttcagcgcg tccggcaacc atcattgcca tgaacatcag gatacctgcc agcaagcaga
    12061 atgttatttc ctgcgatttc cttacattga cgtttttatt actcactgtc ctgttcctgt
    12121 tatcacatta tctgctgaac aattactgat aggttaaaga gaaccaggcc tgggcattgg
    12181 cgatgccgcc agtaacccga cgcccggtag cacgatattt ggcgatgaaa tgtagcgaag
    12241 tagagcctga gtaaagccgt ttccagtttg ctggaggacg attaatcggt acgaggtttc
    12301 cttcatcatc aaacaacgct acgccaatat tggtggctat ccctggcccc tctcccacgg
    12361 aaagcacatc cggattttta ccatccgcga caccgtgaaa cgccacaccc acacgttcac
    12421 tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg caccggtgcg ctatcttccc
    12481 ctaccgcatg aaaccggtta ctgctgattt gccccatatt gaccgtcatt tgtttatcac
    12541 cggcttcaat ccggcaagtt tccgcaataa tgacgccctg aaattgcata tttccgccgg
    12601 gcagcgtggt attccattta tttccggcca gagcaaacat tggcagcaac gacgctaata
    12661 gaaataatct cttccttttc atcgttattt ttatcgcaca agggtgggca tccctgcccg
    12721 taatgacgtc cctgaacctg ggtaggttat tgatactgaa ccttgaaggt cgcatccgca
    12781 ttagcagcac ccggggttgc ggcaccggtt gcaaaataac gcgcctggaa cggaatggtg
    12841 ttggttccgt tattcagggt tgtttctgaa ctaaatgtcg caccgtccag cgccagcgca
    12901 gcacccgttc tgtccaggat ctgcacacca acgtttgttg cgctacccgc agctgaactc
    12961 tgcagagcca gtacatcagt atgcgtagca tcaattgccg tacctaagaa ggcaacagcg
    13021 gcttttgtgg caacagtggt atcgcaatca ttcagctgaa tgttaaaacc aacggcagag
    13081 ctggttgctc cagcctgctt caggctagcg gtacgaacct ggcccaactg aacggtttga
    13141 tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa cttccccttt aaagtgaacg
    13201 gtcccaccat ttaccgtcgt agtatcggcc agagccgctg cggaactgag ggacagagcc
    13261 gacagaacaa cgattgccag agttttaatt ttcatgctgc tttcctttaa aaaactattt
    13321 ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa
    13381 cactgcacag ttttccccca a
//
LOCUS       DAPPNJ010000123        13274 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000123 DAPPNJ010000000
VERSION     DAPPNJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.124"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1007
                     /locus_tag="RPM73_002997"
     CDS             <1..1007
                     /locus_tag="RPM73_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX6521335.1"
                     /translation="QWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDAF
                     RYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVS
                     TPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNW
                     QKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPG
                     RNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKEN
                     RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA
                     "
     gene            complement(1047..2261)
                     /gene="ydcO"
                     /locus_tag="RPM73_002998"
     CDS             complement(1047..2261)
                     /gene="ydcO"
                     /locus_tag="RPM73_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDX6521336.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            2314..2850
                     /gene="sutR"
                     /locus_tag="RPM73_002999"
     CDS             2314..2850
                     /gene="sutR"
                     /locus_tag="RPM73_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDX6521337.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDEQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2923..4884
                     /gene="rlhA"
                     /locus_tag="RPM73_003000"
     CDS             2923..4884
                     /gene="rlhA"
                     /locus_tag="RPM73_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDX6521338.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4976..5206)
                     /locus_tag="RPM73_003001"
     CDS             complement(4976..5206)
                     /locus_tag="RPM73_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDX6521339.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5429..5605
                     /gene="hicA"
                     /locus_tag="RPM73_003002"
     CDS             5429..5605
                     /gene="hicA"
                     /locus_tag="RPM73_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDX6521340.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5651..6067
                     /gene="hicB"
                     /locus_tag="RPM73_003003"
     CDS             5651..6067
                     /gene="hicB"
                     /locus_tag="RPM73_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDX6521341.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV"
     gene            6146..7552
                     /locus_tag="RPM73_003004"
     CDS             6146..7552
                     /locus_tag="RPM73_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDX6521342.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7797..8942
                     /locus_tag="RPM73_003005"
     CDS             7797..8942
                     /locus_tag="RPM73_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX6521343.1"
                     /translation="MSKIFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8960..9973
                     /locus_tag="RPM73_003006"
     CDS             8960..9973
                     /locus_tag="RPM73_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6521344.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9974..10915
                     /locus_tag="RPM73_003007"
     CDS             9974..10915
                     /locus_tag="RPM73_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521345.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10905..11699
                     /locus_tag="RPM73_003008"
     CDS             10905..11699
                     /locus_tag="RPM73_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521346.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11721..13145
                     /gene="patD"
                     /locus_tag="RPM73_003009"
     CDS             11721..13145
                     /gene="patD"
                     /locus_tag="RPM73_003009"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDX6521347.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3237 a         3273 c         3563 g         3201 t
ORIGIN      
        1 cgcaatggct taaagattcg ccctcacagc cattgcaaca gtcactgaaa gaccttgagc
       61 gggcttacaa aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggggac
      121 agaatgatgc attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt
      181 ttctgccgaa actgggctgg atgcgctacc ggaacagccg tcaggtcacg ggtgttgtga
      241 aaaatgtcac tgtcagccag tcctgcggta agtggtacat cagtattcag acagaaagtg
      301 aagtatccac tccggctcac ccttcagcat caatggtcgg gctggatgct ggcgtggcta
      361 aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt cagaaaaacc
      421 agaagacgct ggcgagactt cagcgacagt taagccgcaa ggtcaaattc agcaacaact
      481 ggcagaagca gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat atccgcaggg
      541 actaccttca caaagtcaca acggccgtca gcaaaaacca cgcaatgata gtcattgagg
      601 atttgaaggt cagcaacatg tcaaagtcag cagcgggtac ggtcagccag ccggggcgca
      661 atgtccgggc aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg tatgaaatgc
      721 accgccagct tgagtacaag cagctctggc gtggcggtca ggtgcttgct gttccgccag
      781 cgtatacaag ccagcgttgc gcgtactgtg gtcatacagc gaaagagaac cgcctgtcac
      841 aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta aatggcgctc
      901 gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg cagtcaggcc
      961 gcccgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg tagcagggat
     1021 ccacgtcctt cagggcgtgg aggatgtcaa tatcggtttc tgtcagcgat taaattcaac
     1081 accacgtaac aaacgcctcc ggcaattaat ccccagaacg cagaaccaat cccgaccagc
     1141 gtcaatccac ttgccgttac cagaaatgcc accaccgccg cgtcgcgctc acgctcatta
     1201 tgcagcgcct gatacaaact gccgccaatg gtactcaaca gcgccagacc tgccagcatc
     1261 tggatccaac ttacgggcag ggcagccatc atcccggtaa tggcactacc aaacagacct
     1321 gcgagcaaat agaaaatgcc tgcaacggca gctgccagcc aacgttgatc tttatccgga
     1381 tgcgcttccg ggctttggca aatagccgcg gtgattgccg caataccgac ggaataaacg
     1441 ccgaaagggg aaaaaaccag tgccagcaat ccagtaaata caattaatgg cgaaacagga
     1501 gccgaatatc cagctgcttt cattgctgcg atacccggtg cgttttgcga tgccatcgtc
     1561 accagaaaaa gggggagtgc aacgctcagg ctgtgagcaa acgaaaaatc aggggtaata
     1621 taagtgggga gaacgggttt aaagacaaca tcagttgtga caacgtcacc ttgcgcgatg
     1681 acgatcacga tcccaataat catcgcggca attaccgcat agcgcggcgc aacggccttg
     1741 gttgccagcc ataccagcaa catacttcca cacaacgtaa attgaccgtc cagactggca
     1801 aacgcctgta aaccaaagcg taataaaatc ccggcaagca ttgccgccgc aagcgagtgc
     1861 ggaataatgc gcatcagacg agcaaagagt cccgttatgc cgcagaggac tattaacgcg
     1921 ttggtgacaa taaaaacgcc gatggcttcg ttaagtgtta gtccctgcaa tccggtgacc
     1981 aacaaagccg cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata ccataatgtc
     2041 agagtcagcg tactgacgcc cattgccagc cccagcgccg tcatccagcc agagatttgt
     2101 gcagtggtgg ctccggcgac aatcgctgct tgccagatta ttgccgctga actggcgtag
     2161 ccaattaata ccgccagaaa ccccgccagt agcgtgggtg gaggaataga aaacagacgc
     2221 atagtcacct cgtgcgttat agcgtacagg caccgtagca tttgtccgtt ataacgcaca
     2281 agtgataaac ttccgttttg ccggaggagt cgcatggaaa atctcgctcg ctttttatcc
     2341 accacactta aacaactacg ccagcagcgc ggctggagtc tttcgcgact ggcagaagcg
     2401 acgggcgttt ctaaagcaat gttagggcag attgagcgta atgagtccag tccgacggtc
     2461 gcgacattat ggaaaattgc caccgggttg aacgtaccat tttcaacatt tatttctccg
     2521 ccgcagtccg ctacgccttc agtttatgat ccacaacagc aggcgatggt gatcacttcg
     2581 ctgtttcctt atgatccaca actctgcttc gaacacttct cgattcagat ggcatctggt
     2641 gcaataagcg aatcgacgcc ccatgagaag ggggtaatcg aacatgtcgt cgtcattgat
     2701 gaacaacttg atctgtgcgt tgatggggaa tggcagaccc ttaattgtgg ggaaggcgtt
     2761 cgatttgctg cagacgtcac gcatatctat cgtaatggtg gggagcaaac cgtacatttt
     2821 cattccctca tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa cgtcgcgctt
     2881 gtggctaaaa tagccgccat ttttcagcta ctggataaga atgtgaccgt atcttctcat
     2941 cgacttgaac tgttaagccc ggcacgcgat gccgccattg cccgcgaagc tattttgcac
     3001 ggtgccgatg ctgtttatat cggcggccct ggttttggtg cccgtcataa tgccagtaat
     3061 agcctgaaag atattgccga gctggtgccg tttgcccatc gttatggtgc aaaaattttc
     3121 gtcacgctta acaccatttt gcatgatgat gagctggaac ccgcgcaacg gctgattacc
     3181 gacctctacc agaccggtgt cgatgcgctg attgttcagg atatggggat tctggaactt
     3241 gatattccgc cgattgaact gcacgccagt acgcagtgcg acattcgtac agtagaaaaa
     3301 gcgaagttcc tctctgatgt tggcttcacg cagattgtgc tggcgcgaga gctgaatctt
     3361 gagcagatcc gcgcgattca ccaggccacg gacgcgacca ttgaattctt tattcatggg
     3421 gcactgtgcg tggcctattc gggtcagtgc tacatttctc atgcgcaaac agggcgtagc
     3481 gccaaccgtg gtgattgctc gcaggcgtgc cgtttgccat acacattgaa agacgatcag
     3541 gggcgggtgg tttcctatga aaaacatctg ctgtcgatga aagataacga tcagactgcc
     3601 aacctcggcg cgctgattga tgctggcgta cgctccttca agattgaagg gcgttacaaa
     3661 gatatgagct acgtgaagaa tatcaccgcc cattatcgcc agatgcttga tgccattatt
     3721 gaagaacgtg gcgatctggc gcgcgcttca tcaggtcgta ctgaacattt ctttgttcca
     3781 tcgacggaaa agactttcca ccgtggtagc acagattatt ttgtgaatgc ccgtaaaggc
     3841 gatattggcg cgttcgattc gccgaaattt atcggcctgc cggtaggcga agtattgaaa
     3901 gtggcgaaag atcatctcga tgttgccgtc accgagccac tggcaaatgg tgatggcctg
     3961 aacgtgatga ttaaacgtga agtcgtcggt tttcgtgcca atacggtcga gaaaaccgga
     4021 gaaaatcagt accgcgtctg gcccaatgaa atgccagcag atttgcacaa aattcgtcca
     4081 catcacccac taaaccgtaa tcttgatcat aactggcagc aggcactgac aaaaacctcc
     4141 agcgaacgtc gggtggcggt agacattgaa ctgggcggct ggcaggaaca actgattctg
     4201 accctcacca gtgaagaggg tgtcagcatc acgcatacgc tggacgggca gttcgacgaa
     4261 gccaataacg ccgaaaaagc aatgaacaat ctgaaggatg gtctggcaaa actggggcaa
     4321 accctctatt acgcccgcga tgtgcaaatt aatttgccgg gggcgctgtt tgtaccaaac
     4381 agtctgttaa accagttccg ccgtgaagct gctgacatgc tggatgctgc gcgtcttgcc
     4441 agttaccagc gcggcagccg taaaccggtt gctgatcctg cgccggttta tccgcaaacg
     4501 catctgagtt tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta tcatcgctat
     4561 ggtgtgcagc tgattgacgc ggcgtatgaa gcacatgaag agaagggcga agtcccggtg
     4621 atgatcacca agcattgtct gcgctttgcc tttaatctgt gcccgaaaca ggcgaaaggc
     4681 aatatcaaaa gctggaaggc gacgccaatg caactggtta acggcgatga agtattaacg
     4741 ctaaagtttg attgccgccc atgcgagatg cacgtcattg gcaaaatcaa aaatcacata
     4801 ctgaaaatgc cgttaccggg aagcgtagtg gcatccgtaa gtccggatga gctgctgaaa
     4861 acattgccta agcgaaaagg gtaaaacgcc agttttctgg ttactcacaa ctcattgaat
     4921 ctgcatgata ttgcctgccg ggtaaggcgt ctcgccgcat ccggcaacaa atgactcaac
     4981 gttgtcccgc ttctggtttg cgcgattttt gccagtaaaa atgctcgcgc aaaccttccg
     5041 ccgactcttc cgccacagca cgcaattcat cgctgtccgc ttcatgacgc agctgatgat
     5101 ccacattctt tacccacaca aattcatgtc ctttgtgccc tgccatgagt tgtcctgaga
     5161 acagcgcaca cgttaataag acaaccgata acgccttcgt aaacatcctg ccaccttttt
     5221 tcttaccttt tgccgctatg atgccgatcg tttcttgagg ttattattca gttttgcaaa
     5281 ttagcgcaaa gaaattctgg aatcttcctt cctgattttg catggcattc tgccgttgcg
     5341 gcgatttagt gctattttcg agcaaattac acacggaggt aaacactaat gtttactttg
     5401 ttgatatact cagcggcagg gaggcgatgt gaaacaaagc gagttcagac gttggctcga
     5461 atctcagggc gtcgatgtag cgaatggcag caaccatttg aaactcaggt ttcatgggag
     5521 gcgcagtgtc atgccgcgtc acccctgcga tgagattaaa gaaccattgc gtaaagcaat
     5581 cctgaaacaa ctcggtttga gttaatcgcc aattaaaaag gttaatgaca tgcgagggac
     5641 agtcgaaatt atgcgttatc ccgtcactct tacacccgcg ccggaaggcg gttatatggt
     5701 ttcttttgtg gatatccctg aagcgttgac gcagggcgaa actgtcgctg aagcgatgga
     5761 agcggcaaaa gatgctttac tgaccgcatt tgatttttat tttgaagata acgagcttat
     5821 ccctttacct tcgccattaa atagtcatga tcactttatt gaagtacctt tgagcgtcgc
     5881 ctctaaggta ttgctgttaa atgctttttt acagtcagaa atcactcagc aagagttagc
     5941 caggcgaatt ggcaaaccta aacaggagat tactcgccta tttgacttgc atcatgcgac
     6001 aaaaatcgac gccgtccagc tcgcggcaaa ggcgcttggc aaagagttat cgctggtgat
     6061 ggtttaatta cagttaacga aaagttgtca tttttaacaa ctgatataga ctgccgaatc
     6121 atctgcacat aattacgatt cgataatgaa aaaataccag cagcttgcag aacaattacg
     6181 cgagcagatt gcgtcgggta tctggcaacc cggcgatcgt ttgccttcgt tgcgtgacca
     6241 ggtggcgctt tctggcatga gctttatgac tgtcagccat gcctatcagt tgctcgaaag
     6301 tctgggatat attatcgcgc gaccgcagtc gggttattac gttgcgccac aggcaataaa
     6361 aatgccgaaa gcgccagtca ttccagtcac tcgagatgaa gcagtcgata tcaacactta
     6421 tatttttgat atgttgcagg ccagtcgcga tccgtcggtc gttccgtttg cctcggcctt
     6481 tcccgacccg cgacttttcc ccctccaaca actaaaccgc tcgctggcgc aggtaagcaa
     6541 aaccgccaca gcgatgagcg tgattgaaaa cttaccgcca ggaaacgcag aactgcgtca
     6601 ggctattgct cgtcgctatg ccttacaggg catcaccatt tctcctgatg aaattgtcat
     6661 tactgccggg gcgttagagg cattaaacct cagtttgcaa gcggtaactg aaccgggcga
     6721 ttgggtgata gtagagaatc cttgtttcta cggtgcgttg caggcgctgg agcggctacg
     6781 gctgaaggcg ttatcggtgg cgacggatgt taaagaaggg atcgatcttc aggcgctgga
     6841 actggcgttg caggattatc cggtgaaagc gtgctggctg atgactaata gccagaaccc
     6901 actcggattt accttaacgc cgcaaaaaaa agcacaactg gtggcgttac tcaatcagta
     6961 caacgtaacg ctgattgaag atgacgttta cagcgaactt tattttggac gggaaaaacc
     7021 gctgcctgcg aaagcgtggg atcgccacga tggcgttttg cattgctctt cgttttcgaa
     7081 atgtctggtg cctggttttc gtattggttg ggtcgccgcc ggaaaacatg cacgtaaaat
     7141 tcaacgcttg cagttgatga gtacgctttc caccagctca ccgatgcaac ttgcgctggt
     7201 ggattacctt tccacgcgcc gatacgacgc ccatcttcgt cgcctgcgtc gccagcttgc
     7261 ggaacgtaaa caacgtgcct ggcaggcact gctgcgttat ctgcctgcgg aagttaaaat
     7321 tcatcatagt gacagcggtt attttctctg gctggagctc cccgagccgt tagatgccgg
     7381 cgaattaagc ctggcggcac tgacgcatca tatcagtatt gcgccgggta aaatgttttc
     7441 taccggtgaa aactggtcac gtttttttcg ttttaatacc gcgtggcagt ggggagaacg
     7501 tgaagaacag gcggtaaaac aattaggcaa acttattcaa gaacggctgt aatagcgttt
     7561 aatttaattc ctcttagatt gggtaatatg aatttcgaat agcagtcata tttcctaacc
     7621 ccttgtctat actccagaag ataaccttac agacggcata atgcgcggta gctcacaacc
     7681 tgattaaatt ttctcagggg cgaaggtgtg cctgcaagcc gccgtctatg gttaaacaag
     7741 gagatatttt tacggcacgg cggctgaaca attaattacg acaggagtaa gaccttatga
     7801 gcaagatatt tgcccgcagc agcctgtgtg cgctcaccat gacaataatg accgctcacg
     7861 ccgccgaacc gcctaccaat ttagataaac cggaagggcg actggatatt atcgcctggc
     7921 cgggatatat cgaacgcgga caaactgata aacaatacga ctgggtaacg caattcgaaa
     7981 aagagacagg ctgcgcggtg aatgttaaaa ccgccgcgac ttccgatgaa atggtcagtc
     8041 tgatgaccaa agggggttac gatctggtta cggcatccgg cgatgcctcg ctgcgtttga
     8101 ttatgggcaa acgcgtgcag ccgattaata ccgccttgat tcccaactgg aaaacgctcg
     8161 atccgcgcgt ggttaaaggc gactggttta atgttggcgg caaagtttac ggcacacctt
     8221 accaatgggg gccgaacctg ctgatgtaca acactaaaac cttcccgacg ccgccggata
     8281 gctggcaagt agtttttgtt gagcaaaatc tgccggacgg caagagcaat aaaggccgcg
     8341 ttcaggctta tgatggccct atctacattg cggacgctgc gttgttcgtt aaagccactc
     8401 agccgcagtt gggcatcagc gatccatatc aactcaccga agaacagtac caggcggtgc
     8461 tgaaagtgct gcgcgctcaa catagtttga tccatcgcta ctggcatgac actaccgtgc
     8521 aaatgagcga tttcaaaaac gagggtgtgg ttgcttccag tgcatggccc tatcaggcca
     8581 acgccctgaa agccgaaggc cagcctgtcg ctaccgtttt cccaaaggag ggcgttaccg
     8641 gttgggctga taccaccatg ctgcatagcg aagcgaaaca tccggtttgc gcctacaaat
     8701 ggatgaactg gtcattaacg ccaaaagtgc agggcgatgt ggcggcctgg tttggctcgt
     8761 taccggtagt gccggaaggg tgtaaagcca gtccgttatt gggcgaaaaa ggttgtgaaa
     8821 caaacggttt taactatttc gacaaaatcg ccttctggaa aacgcctata gcagaagggg
     8881 gcaagtttgt tccctacagt cgctggacgc aggattacat tgccattatg ggcggtcgct
     8941 aacttcgctg gggtgcttta tgacgtacgc agtggagttt gacaacgtct cgcggttgta
     9001 cggtgacgtg cgcgcagtag atggcgtcag tattgcgata aaagatggtg agttcttctc
     9061 tatgctgggg ccgtccggct ccggcaaaac cacctgcctg cgcctgattg ctggcttcga
     9121 acagctttcc ggcggggcta tctctatctt tggtaaaccc gccagcaatc tgccaccgtg
     9181 ggagcgggac gtgaataccg tctttcagga ctacgcgcta ttcccgcata tgtcgattct
     9241 tgacaatgtc gcctatgggc tgatggtcaa aggcgtgaat aaaaagcagc ggcacgcaat
     9301 ggcgcaagag gcgctggaga aagtggcgtt ggggtttgta cagcaacgta aaccgtcaca
     9361 actttctggt ggtcagcgcc agcgggttgc tatcgccaga gcattggtga atgaaccgcg
     9421 cgtattgctg ttggatgaac cgctcggcgc actggatctc aaattgcgtg agcagatgca
     9481 gctggaactg aaaaaactgc aacagtctct cggtatcact tttatcttcg ttacccacga
     9541 tcagggcgaa gcgttatcga tgtccgatcg tgtggcggtt ttcaataatg gacgcattga
     9601 gcaggtcgat tccccgcgcg atctctatat gcgcccgcgc acgccgtttg ttgcgggatt
     9661 cgttgggaca tcgaatgttt ttgatggact gatggcagag aaactttgtg gcatgacggg
     9721 aagcttcgcc ctgcgaccgg aacatatccg cctcaacacc cctggtgaac tgcaggccaa
     9781 tggcacgatc caggcggtgc aatatcaggg cgcggcaact cgttttgaac tgaaattgaa
     9841 cggcggtgaa aaactgcttg tgagtcaggc caatatgaca ggcgaagaac tgcctgccac
     9901 actcacgccc ggacaacagg tgatggtttc ctggtcgcgt gacgtgatgg tgccgctggt
     9961 tgaggagagg tgaatggcga tgaacgtatt gcaatcacct tcacgtccag gtctggggaa
    10021 ggtgtccggt ttcttttggc gtaatccggg gctggggctg tttttgctgc tgcttggccc
    10081 gctaatgtgg tttggcattg tctatttcgg ctcgctgcta acgctgttat ggcagggctt
    10141 ttataccttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg cgaatatccg
    10201 tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta tggctgtggc
    10261 agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg cgcgttatac
    10321 cagcgggaaa atgaaagcgt ttttttatat tgcggtaatg ttgccgatgt gggcgagcta
    10381 cattgttaaa gcctatgcct ggacgttatt gctggcaaaa gatggcgtgg ctcagtggtt
    10441 tttacaacat cttgggctgg aaccactgct gactgcgttc cttacattac ctgcggtggg
    10501 cggcaatacg ctgtcaactt ccgggctggg gcgctttctg gtgtttctct atatctggct
    10561 accgttcatg atcctgcccg tgcaggcggc gcttgagcgt ttgccgccgt cattgttgca
    10621 ggcgtcggct gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct
    10681 ggcaatcccg ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt
    10741 tatcgtcccg cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca
    10801 gcagggggcg attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct
    10861 catcgcactg tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg
    10921 caccgttttt cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc
    10981 tgataatcgc cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg
    11041 gcctgacgct gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga
    11101 cactgtcact taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg
    11161 cagctgccgc gctgtggcga cgagactttt tcggcaaaaa cgccatttcg ctgttactgc
    11221 tgctgcccat tgcgctgccg ggcattgtca ctggtctggc gttattaacc gcctttaaaa
    11281 ccatcaatct ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgtgtag
    11341 ttgtggtgtt taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg
    11401 cgtcaatgga tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc
    11461 tcagttcggc gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca
    11521 tcgttacgac ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc
    11581 ttgggcgacc gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa
    11641 caaccttgcc gatcctgggg gcctggtggc taacccgcga aggcgacaat ggtcaataac
    11701 cactgataca ggaatatgct atgcaacata agttactgat taacggagaa ctggttagcg
    11761 gcgaagggga aaaacagcct gtctataatc cggcaacggg ggacgtttta ctggagattg
    11821 ccgaggcatc cgcagagcag gtcgatgctg ctgtgcgcgc ggcagatgca gcatttgccg
    11881 aatgggggca aaccacgccg aaagtgcgtg cggaatgtct gctgaaactg gctgatgtta
    11941 tcgaagaaaa tggtcaggtt tttgccgaac tggagtcccg taattgtggc aaaccgctgc
    12001 atagtgcgtt caatgatgaa atcccggcga ttgtcgatgt ttttcgcttt ttcgcgggtg
    12061 cggcgcgctg tctgaatggt ctggcggcag gtgaatatct tgaaggtcat acttcgatga
    12121 tccgtcgcga tccgttgggg gtcgtggctt ctatcgcacc gtggaattat ccgctgatga
    12181 tggccgcgtg gaaactggct ccggcgctgg cggcagggaa ctgcgtagta cttaaaccat
    12241 cagaaattac cccgctgacc gcgttgaagt tggcagagct ggcgaaagat atcttcccag
    12301 caggcgtgat taacgtactg tttggcagag gcaaaacggt gggcgatccg ctgaccggtc
    12361 atcccaaagt gcggatggtg tcgctgacgg gctctatcgc caccggcgag cacatcatca
    12421 gccataccgc gtcgtccatt aagcgtactc atatggaact tggtggcaaa gcgccagtga
    12481 ttgtttttga tgatgcggat attgaagcag tggtcgaagg tgtacgtaca tttggctatt
    12541 acaatgctgg acaggattgt actgcggctt gtcggatcta cgcgcaaaaa ggcatttacg
    12601 atacgctggt ggaaaaactg ggtgctgcgg tggcaacgtt aaaatctggt gcgccggatg
    12661 acgagtctac ggagcttgga cctttaagct cgctggcgca tctcgaacgc gtcagcaagg
    12721 cagtagaaga ggcgaaagcg acagggcaca tcaaagtgat cactggcggt gaaaagcgca
    12781 agggtaatgg ctattactat gcgccgacgc tgctggctgg cgcattacag gacgatgcca
    12841 tcgtgcaaaa agaggtattt ggtccagtag tgagtgttac gcccttcgac aacgaagaac
    12901 aggtggtgaa ctgggcgaat gacagccagt acggacttgc atcttcggta tggacgaaag
    12961 atgtgggcag ggcgcatcgc gtcagcgcac ggctgcaata tggttgtacc tgggtcaata
    13021 cccatttcat gctggtaagt gaaatgccgc acggtgggca gaaactttct ggttacggca
    13081 aggatatgtc actttatggg ctggaggatt acaccgtcgt ccgccacgtc atggttaaac
    13141 attaagttag atgttagtgg ctgttatagg acggccacta tcgttttagg ttctgagttt
    13201 aatggcggtt aagtattaga atgattacat taataactag cgtaaaaatt aggctaaatg
    13261 tttgatggcg attt
//
LOCUS       DAPPNJ010000124        13238 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000124 DAPPNJ010000000
VERSION     DAPPNJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.125"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            348..2585
                     /gene="nrfB"
                     /locus_tag="RPM73_003010"
     CDS             348..2585
                     /gene="nrfB"
                     /locus_tag="RPM73_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDX6521348.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2572..5544
                     /gene="nfrA"
                     /locus_tag="RPM73_003011"
     CDS             2572..5544
                     /gene="nfrA"
                     /locus_tag="RPM73_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDX6521349.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     LRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5545..6435
                     /locus_tag="RPM73_003012"
     CDS             5545..6435
                     /locus_tag="RPM73_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDX6521350.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6618..7379
                     /gene="envY"
                     /locus_tag="RPM73_003013"
     CDS             6618..7379
                     /gene="envY"
                     /locus_tag="RPM73_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDX6521351.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7425..7501)
                     /locus_tag="RPM73_003014"
     tRNA            complement(7425..7501)
                     /locus_tag="RPM73_003014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7465..7467),aa:Arg,seq:tct)
     gene            7744..8376
                     /gene="fimZ"
                     /locus_tag="RPM73_003015"
     CDS             7744..8376
                     /gene="fimZ"
                     /locus_tag="RPM73_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDX6521352.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8379..8894)
                     /gene="sfmF"
                     /locus_tag="RPM73_003016"
     CDS             complement(8379..8894)
                     /gene="sfmF"
                     /locus_tag="RPM73_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDX6521353.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(8905..9912)
                     /gene="fimH"
                     /locus_tag="RPM73_003017"
     CDS             complement(8905..9912)
                     /gene="fimH"
                     /locus_tag="RPM73_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDX6521354.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRARPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9925..12534)
                     /locus_tag="RPM73_003018"
     CDS             complement(9925..12534)
                     /locus_tag="RPM73_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDX6521355.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12565..13233)
                     /gene="fimC"
                     /locus_tag="RPM73_003019"
     CDS             complement(12565..13233)
                     /gene="fimC"
                     /locus_tag="RPM73_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDX6521356.1"
                     /translation="MLIIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIRNSHT
                     NERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMN
                     VKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVH
                     NPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVRE
                     VNLN"
BASE COUNT         3435 a         3353 c         3195 g         3255 t
ORIGIN      
        1 gttaagacgt aagatgcgaa aagcagccat ggacagaaac tatctcgcgt cagtccttgc
       61 ggggagcggg ctttcgtaat cttgccctgc ctgctcacct cctccaggca cttccactac
      121 attatgacct gctcggtgcc accgctcctt ttcactatga cgttaacctc ccctccttga
      181 caccatgctt ttgtctacat tctacgcaaa cggcaacttt tataaccagt gtaaaaataa
      241 cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt cacaattcat aaaaaacctt
      301 aatatacgcc accctaaaca taaccagcgt taatgtaagg tttttgtgtg gactggcttc
      361 ttgatgtttt tgcaacctgg ctctacggct taaaagtaat cgcgataacg ttagcggtca
      421 tcatgttcat cagcgggctg gacgattttt ttattgatgt cgtctactgg gtacgccgca
      481 ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat gagttaccgc gaactgtata
      541 aaccagatga aaaaccgtta gcgattatgg ttccggcgtg gaatgaaacg ggcgtcatcg
      601 gcaatatggc cgagctggcg gcgaccacgc tcgactacga aaactatcat atctttgttg
      661 gcacctaccc caacgacccc gatactcaac gtgatgttga cgaagtgtgc gctcgctttc
      721 cgaacgtgca taaggtagtc tgcgcgcgtc ctggtcccac cagcaaagcc gactgtctga
      781 acaacgtgct ggacgccatc acccagtttg aacgtagcgc caatttcgct tttgctggtt
      841 ttattctgca tgacgccgaa gatgtgattt caccgatgga attgcgtctg ttcaactatc
      901 tggtcgagcg taaagatctg attcagatcc cggtgtatcc gttcgaacgc gaatggacgc
      961 acttcaccag catgacttac attgatgagt tttcagagct gcatggcaaa gatgttccgg
     1021 tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt cggcacctgt ttcagccgcc
     1081 gcgccgtgac cgctctgtta gctgacggtg acggtattgc tttcgacgtg cagagtctga
     1141 ctgaagatta cgacattggt ttccgcctga aagagaaagg tatgacggaa atttttgtcc
     1201 gttttccggt ggtggacgaa gccaaagaac gcgagcagcg taaattttta cagcacgcac
     1261 ggacgtcaaa catgatctgc gtgcgcgaat atttccccga taccttttcg actgcagttc
     1321 gacaaaaatc tcgctggatc atcggcattg ttttccaggg ctttaaaacc cacaaatgga
     1381 cctccagcct gacgctgaac tactttctct ggcgcgaccg caaaggggca atcagtaact
     1441 ttgtcagctt cctcgcgatg ctggtgatgc tccagctttt gctgttgctg gcgtatgaaa
     1501 gtttgtggcc caatgcctgg catttccttt ctatttttag cggcagcgca tggttaatga
     1561 ccctgctgtg gctaaacttt ggcttgatgg ttaaccgcat cgtgcagcgg gtgattttcg
     1621 tcactggcta ctacggtctg acgcaggggc tgctatctgt cctgcgtctt ttctggggca
     1681 acctgattaa cttcatggcc aactggcgcg cgctaaaaca ggtacttcaa cacggcgatc
     1741 cacgtcgcgt cgcgtgggat aaaacaacgc atgacttccc cagcgtgact ggcgataccc
     1801 gctcgttgcg cccgttaggc caaattctgc tggaaaatca ggtcatcact gaagaacaac
     1861 tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct gggcggttca atgctgatgc
     1921 aggggctgat tagcgccgag cagctggcac aggcgctggc agagcaaaac ggcgtggcgt
     1981 gggaatccat cgatgcctgg cagatcccgt cctcgctgat tgccgaaatg ccagcctccg
     2041 tagcgctgca ttatgcagta ctgccgctgc gtctggaaaa tgatgagtta attgtcggca
     2101 gtgaagatgg tattgacccg gtttcgctgg cggccctgac gcgtaaagtc ggacgcaaag
     2161 tgcgttacgt cattgttctg cggggacaaa ttgtcacggg gttacgtcac tggtatgcac
     2221 gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc ggttcagcat cagtggctca
     2281 cggaacagca gaccggtgaa atctggcggc aatatgtgcc gcatcagttc ctgttcgccg
     2341 aaatactgac cacgctcggt catattaatc gttcagcaat taacgtgttg ttattgcgcc
     2401 atgaacgcag ttctctgccg ctcggcaagt ttttggtcac cgaaggcgtt atcagccagg
     2461 aaacgttgga tcgcgtcctg acaattcaac gcgaattaca agtttcgatg caatcactat
     2521 tactcaaagc aggtttaaac acagaacagg ttgcgcaact ggagtccgaa aatgaaggag
     2581 aataacctta atcgcgtcat cggatggtct ggtttactgc tgacgtcttt attgagtacc
     2641 agcgcactcg cagacaatat cggcaccagc gcagaagagc tggggctgag cgattatcgc
     2701 cattttgtta tttatccccg tctcgataag gcgctgaagg cacagaaaaa taacgacgaa
     2761 gcaaccgcca tccgcgaatt tgaatatata caccagcagg tgccggataa tattccgctg
     2821 actttatacc ttgcggaagc ctatcgccat tttggtcatg atgaccgggc gcggctgttg
     2881 cttgaggatc aactgaaacg tcacccagga gatgcccgac ttgagcgcag tctggcggct
     2941 attccggttg aagtgaaaag cgttacgact gttgaagaac tgcttgccca gcaaaaagcg
     3001 tgcgacgctg cgccgaccct gcgttgtcgc agtgaagtcg ggcagaatgc cctgcggctg
     3061 gcacaattac ctgtcgccag agcgcaactg aacgatgcga cgtttgctgc atcgccagaa
     3121 ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct acctgaaaca atggtcccag
     3181 gcagatacgc tatacaatga agcacgccag cagaacacat taagcgcggc agaacgccgt
     3241 cagtggtttg acgtgcttct tgccgggcag ctggacgatc ggatcctggc actgcaatca
     3301 caggggatct tcaccgatcc tcagtcatat attacttacg cgaccgcgct ggcttatcgt
     3361 ggcgaaaaag cacgcctcca gcattatctc aatgaaaata agccgctgtt taccacggac
     3421 gcacaagaga aaagttggct ctatctgtta tctaaataca gcgccaaccc cgttcaggca
     3481 ttggcgaatt atacggtgca gtttgccgat aaccgccagt atgttgttgg cgcgacgcta
     3541 ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa aactgctcgc caccctcccc
     3601 gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg cgacccgtaa caaggctgaa
     3661 tctctgcgtc tggcacgatt gctgtatcag caagaaccgg caaatcttac ccgcctggat
     3721 caactgacct ggcaactgat gcagaacgag cagtcacgcg aagctgccga tttattgctg
     3781 caacgctatc ctttccaggg cgatgcgcgt gtcagccaga ctttaatggc gcgactggcg
     3841 tctctgctgg aaagtcatcc ttacctggca acgccggcga aggtggcgat tttatcgaaa
     3901 cccttaccgc tggcggagca acgtcagtgg caaagtcagt tgccgggtat tgcagataat
     3961 tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt cctacgatgc tgccgcctgg
     4021 aaccgtctgg caaagtgtta tcgggacacg ctacccggtg tggcgctgta tgcatggctt
     4081 caggccgaac aacgacaacc gaacgcctgg caacatcgtg cggtagccta tcaggcgtat
     4141 caggttgagg actacgccac cgcactggcg gcctggcaga aaatcagtct tcacgacatg
     4201 agcaatgagg atctgcttgc tgctgccaat accgcccagg cggcaggaaa tggtgcagct
     4261 cgcgatcgct ggctacaaca ggcagaacaa cgtggactgg gaaacaatgc cctctactgg
     4321 tggctgcatg cgcaacgtta cattcctggt cagccggaac tcgcactgag cgatctcacg
     4381 cgctcaatca atattgcgcc ttctgccaac gcttacgttg cgcgggcgac aatttatcgc
     4441 caacgtcata atgtcccggc ggcggtgagt gatttgcgcg ccgcgctgga actggaaccg
     4501 aataatagca acatccaggc agcgcttggt tacgccttgt gggatagcgg tgatatcgca
     4561 cagtcgcggg aaatgctcga acaggcgcat aaagggcttc cggacgatcc ggcactgctc
     4621 cgacaactgg cctacgtgaa ccagcgtctg gatgacatgc ctgcgacgca gcactacgcc
     4681 cggctggtga ttgatgacat tgataatcag gcgctgataa ccccactgac cccagagcaa
     4741 aatcagcaac gcttcaattt ccgccgtttg catgaggagg tcggtcgccg ctggacgttc
     4801 agtttcgatt cttctatcgg cttgcgttcc ggggcaatga gcaccgctaa caataatgtc
     4861 ggcggcgcag cgccagggaa aagctatcgt agctacgggc aactggaagc cgagtaccgc
     4921 atcggacgca atatgctgct ggaaggcgac ctgctctcag tttatagccg cgtctttgcc
     4981 gataccggag aaaacggggt gatgatgccg gtgaaaaatc cgatgtccgg caccggtctg
     5041 cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg tcgaacagca gttgccgctg
     5101 aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca gcgcctcatt ctttaatggc
     5161 ggcaaataca gcgacgaatg gcacccgaac ggttcaggct ggtttgccca aaacctgtac
     5221 ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt ggacggcaga ttatcgcgtc
     5281 agctggcatc agaaggtagc taacggacag actattgagc cttacgctca cgttcaggac
     5341 aacggctatc gtgataaagg cactcagggc gcgcagcttg gcggagtcgg ggtccgctgg
     5401 aatatctgga ccggcgagac gcactacgac gcctggccgc acaaagtcag tctcggcgtc
     5461 gagtatcaac atacctttaa ggcgattaat caacgtaacg gagagcgcaa caacgcgttt
     5521 ctcaccattg gagtgcactg gtaaatgcgt aagttcattt tcgtattgct gacactgctt
     5581 ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct ggcaaccaca aaaccgagat
     5641 agtcaggtta ccgataccca gtggcagggg ctgatgagtc agttacgttt gcaaggcttc
     5701 gataccctgg ttttgcaatg gacccgttac ggcgatgcat tcacccagcc agaacagcgc
     5761 gcgttattgt ttaagcgtgc cgcagctgcg caacaggctg gcctgaagct gattgtcggg
     5821 ctgaacgccg atccggaatt ttttatgcac cagaaacagt cgtccgcagc gctggaaagc
     5881 tatcttaatc gcctgctggc tgccgatctc cagcaagcca gattatggag cgccgcgcct
     5941 ggcgtaacgc cggatggctg gtacatcagc gcggaaattg acgacctgaa ctggcgcagc
     6001 gaagctgccc gtcagccttt gctaacatgg ttaaacaacg cgcagcggct gattagcgat
     6061 gtttcagcaa aaccggttta tatcagtagt tttttcgccg gaaacatgtc gcccgatggc
     6121 tatcgccaac tgctggaaca cgttaaagca accggcgtta atgtttgggt acaggatggc
     6181 agcggcgtgg ataaactgac cgctgaacag cgtgaacgtt atctacaggc cagcgccgat
     6241 tgccaaagtt ccgccccagc aagcggcatt gtttatgaac tgtttgtcgc cggcaaaggc
     6301 aaaaccttta cagcgaaacc gaaaccggac gcagagatcg cctcgctgtt agcgaaacgt
     6361 tcctcttgcg gtaaagacac tctctatttc tctctgcgct atttgcccgt cgcgcacggc
     6421 attctcgagt attaaatctc ctccaggtaa gtcgggtacg acctggctta cctctttcgc
     6481 tctcgtcaga attccatcaa gattaattgc taaaaagcgt gctaaagaaa gaagatcgtt
     6541 atcggcgaac gggccgatca ctcatgcagg aagcatagat cctttgagcg gagtttctga
     6601 tatctgagga gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg atcctgaccg
     6661 aaaaagaggt agaggtaagc gtcaataacc atgctacgtt tacccttccg aaaaactacc
     6721 tggccgcctt cgcgtgcaac aataacgtca ttgaactctc aacgttaaat cacgtattaa
     6781 tcacccacat caaccgtaac atcatcaacg attatctgtt gtttttaaat aagaacttaa
     6841 cctgtgtaaa gccctggtcg cggctggcaa ccccggttat tgcttgtcat agccgtacac
     6901 cggaagtgtt ccggctagcc gccaaccaca gcaagcagca acccagcaaa ccctgcgaag
     6961 cggagttgac gcgcgtattg ctttttaccg tattgtctaa ctttcttgag caatcgcggt
     7021 ttattgccct actgatgtat atcttacgca gcagcgtccg cgacagcgtc tgccgcatta
     7081 ttcaaagcga tattcagcat tactggaatc tgcgaattgt cgccagttcg ctatgtttaa
     7141 gccccagcct gctcaaaaag aaattaaaaa acgaaaatac cagctatagc cagattgtca
     7201 cagagtgtcg tatgcgttac gccgtacaga tgttattgat ggataacaaa aatatcactc
     7261 aggtggcgca attatgtggc tatagcagca cgtcatactt tatctctgtt tttaaggcgt
     7321 tttacggcct gacaccgttg aattatctcg ccaaacagcg acaaaaagtg atgtggtgaa
     7381 gggcaaagcg gaaacggata agacgggcat aaatgaagaa gaaatggcgc gccctgcagg
     7441 attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga gctaagggcg
     7501 cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt gctgagtcaa
     7561 tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct gatttatttc
     7621 gctatagcgg caaacaaaca cacaccgctg cgcgtctgaa tcaagaaaac ccgtattttc
     7681 atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat gagactggta
     7741 actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag aatgtctatt
     7801 gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga tgattaccgc
     7861 ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga tatagacttg
     7921 cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag cacagtgaaa
     7981 gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat acaagctggt
     8041 gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc cgttcagatg
     8101 atcctctccg gatacacgtt ttttcccagc gaaacgctta actatataaa aagcaataaa
     8161 tgtagtacga atagttcaac ggtcactgtg ctatctaatc gtgaagtgac catattacgt
     8221 tatctggtta gcggattatc taataaagaa attgccgata agttattact tagcaataaa
     8281 acagttagtg cgcataaatc taatatttat ggcaagctag gtttgcattc aattgtagag
     8341 cttatcgact acgccaaatt atacgaatta atataatatt aattataatt gatcataaat
     8401 atcgcatccg ctttcgccac acctggccga acaccgctgg ctaacgctcg ataattggca
     8461 aaaaagttta gtgtgacatt gccatttgca tctacttcct cagtcgggct cgccgctccc
     8521 agtgcgagcc gggagcgatc gctattacgt aattcgatgg cgatggtttg cgccattgcg
     8581 ggatcatcca gagccagcag gttggtatcg gatgccggcg ttcccgtaaa taaaatcgca
     8641 actgaacccg gaggacatcc ctccagccgc aggctaaaag ggacgagtgc cgtggtatcg
     8701 ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat ctaccgtctt atcaatatcc
     8761 gcagtgttta cggtacagga gaaatcaaca acgttaccgt gcaaattgat attaatcgtt
     8821 cccaaagggt cagctgccca tccactggaa atccacagta acccgcagaa acagctaaag
     8881 agtgctcttc tcattctccg tacctcattg ataatcgacg cgtaaatacc ccagcgcgct
     8941 aaacggccct tcggtcggtt tttgaccggt aatactgata ggcctggcgc gaagtgtgac
     9001 attggctgcc gcagctgcat ccagacggaa aggaataacg ctattgagat cgttaggcgt
     9061 gatcggcgta tcgttctgat cggcgacaat aaaacctaaa tcctgattgt ccgacaccat
     9121 cgcctgacca gaaacggcac tggcttccag acgcattgtt aaataagcct gcgcagcaac
     9181 attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg acaccagcag gtcgattacc
     9241 cggccctgcc gcactaaata acgatgcgcc gatatcacca aaatcaaatt caacaatctg
     9301 cccggcattt aattcgcagt tttgcggtac ttcaacccgg ccaccaaaac taatggtata
     9361 aacaggtgta accaacggat cgtaaggcgt tgatgtcaca tacacggtaa acatcacctg
     9421 tctggggatc tccaccatgt taataaaggg acgaataacc tttaaacgaa atattaaacg
     9481 agaatccatc accccgaatg gaaggttttg tgaaacgtta ggatctgttc ccatccgaat
     9541 ataattttgc ggggggaact gaatatccat cacactatca accagactca tcgcacctaa
     9601 tagataatca tttaattgca tatatttata attatcgata gtttcctgaa caataaagtt
     9661 ggtgacataa ctacggtatg tataattcac cagcgtgccg ggtggacaaa ttgctgacac
     9721 acctacccag ccggattttt ccggtaaaac aacaatctgc cctggctggt tattggtgct
     9781 attaaagata tttgagagat catagtgttc atcttctgct acgccagtac tatttgcaca
     9841 aacagtagcc caggatatgt ttgccagaca caaacatgcc atcgccaata ataatctgca
     9901 gattatcttc attgataatt tcctttatga gccaggatgt gtgcaaactg ccgaaataac
     9961 agtgacggct tgctgtaagc tttgcttcgg caatacatag tgcgcaatgc agcgtgagtt
    10021 cgcgccgtct ccccactgaa ccagtaattc accagacaat ggcgcaccac ttaaataaac
    10081 ttgcccgtca tcacccacca tactggttat tccggtactg ttttcccgta ccagtgatcc
    10141 aaacggcacc ggttttccgc cctgggtaac ggtaatgagc gcccgcacgc ctatgcgggt
    10201 atcaaaattg gcacgaacca acgcgccttg cgtcggcaca acgctgctga tatttttttc
    10261 aacatcgatg gaattcccca tcgtattggt atcaagcgcg atacggttat accgataaac
    10321 cgtggcatac ggcatcaccg catagccgcg ccagtcggtt aaaatgccag tttgattttc
    10381 aatgcgtaca ccgcctgcgc caggcgcttt aatcaaaaca ttggtatccc ctaaaggctg
    10441 gctcagcgtt atgccatttt cgtgcccgac cacaccgcca gacagctgcc agttaacgtc
    10501 atgttgatcg cggtcgtagt tatacccgac ccccagcgta ccgtaagcgg cctgccagtt
    10561 tgccgtggcg ctgcccgtat acccattatt cgaggtatca ccctggctta cgtgataact
    10621 caggttgtgg ccttctagta aggtcccacc tacacctgcc agccagctat tttgcccgtt
    10681 gctgttacgg ttggcgttaa aggaggcata agcgcggtcg agcgcatttt cccgggtgta
    10741 gcgacgtttg gtcagaacat tgaaaggcac tgaaacatta agtccgacaa tacgttcgtt
    10801 atcggggatc cctacagatt cattccacga aaatgagagc gaataactga tgccaaccca
    10861 gctgctggta taccccacct gataccaggt atctgaatcc gaagtattcc agtatttttg
    10921 atgggtacca gagatataaa gcgagccaaa gtcattaagt gattgtgaaa tatttaactg
    10981 caaacggtct ttacggctaa agcgtaaatt gtggtaattc acgattatca gttcatcccg
    11041 atgctcaccg tcgtaatcgt aatcatattc gtacccctcc attcgacgat acgcaacatc
    11101 atcaagggta taaaaacctt gtgtcgaata gcggtaaccc attaactgaa aattggtgcc
    11161 gaaggtgttc atcgatttcg catagaggaa gcgaatggaa tccccctcat gacgactgtc
    11221 gtcggctaac tgactgcgcg catgggtcac atccagcgac actgcccccc agtttccgag
    11281 attgcgcccc agtcctaata aaaaggcggt gtaattggca gataattgcg tcccgccgta
    11341 ggcagtaaat tcctgtggta aaccgccgag tgccgtgccc tgaaaaaaga aaggtgatga
    11401 ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg tcaaatttga aacgcccttc
    11461 gcgttgtaaa atcggcactg ttgaatacgg aattgtgtaa ttctgctgat tgccatcgcg
    11521 ctcgtcgatg gtgacatcca gatcgccatt tgaagatgtc gggtgcaaat ctgtaatttc
    11581 aaaagcgccg ggggaaacat agctttgata gataataaaa ccattttgcc gaatcgttag
    11641 ctgggccgcc gtacgggcaa tcccacgtac cgttggggca aacccttgct ggctatcagg
    11701 atacatatta tcggaagaat aaagccgtac accacgaaat ccaacaccat cgaaaatatc
    11761 acttcctgta ttgccgtctc ccattaccag ttcacttttc agcggaataa tggcgcgctg
    11821 tacccaggtg ccaatattat tccactgttc tgaatgatat ccatttccgc gaaaatagtt
    11881 ccaggaacca ttgttgcgta aacgccacgg gccaatatta atcccgctga gctcactaaa
    11941 aaaatagcta tcgttaccat tacctctgtt accggtgaaa ttataattca ggagtaaagc
    12001 aggaatacct tcatcccact cttcaggcgg aatgtaaccg tgagcgctac tcaacaacgt
    12061 tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa tcaaagttaa ttgtggcatc
    12121 aggtacagct ttcagtaaat tgatgcattt attgttttgc tgctgggcta actcgggaaa
    12181 tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg ttaaaacagg gcattaaccc
    12241 acctgattta tcacctgtat tttctgttgt cgattcaaat acgatatcct gtgaaccaat
    12301 gaactcatca ttacgccaga gatccacccg atacactccc gcaggttgat gattgcctct
    12361 ctcaaagcgc gataaatcag caacggatgt gccattctct aataaaaatg ccgggttgaa
    12421 ataactttcc gcatagctta aaaaggaggg taaaatggca agagatgaat aacaaattcc
    12481 ggccagacac caggtatacc tctgcggaat atccgtagta gtgggtattt tcataattag
    12541 ctctgatatt gtcagatgat tcggttagtt taagttcact tctctgaccg gggttactga
    12601 accataatca ttaacggtct gaaatttcag ctttccctgc actcctgagg gtaagggaat
    12661 ttgtgaatta actctgggtg cagccatagc attccccaac ttttggctgc ccacttgtaa
    12721 gttaaccagg gtgacataaa aaggtgaggg attatgaaca ttgatatagt taccggatcg
    12781 cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct tgtaattgaa ttggacggag
    12841 aaataatttc atgcgcgata aaatcgccag ttgcaaaaca ttcctgccgt tcaatgcatt
    12901 tttatctacc gaagggatcg tcttaacatt catccagaac agagactcac gatctgccgc
    12961 cagcggtgga ccggtgtaaa taatacgcaa ggtattttcg cttttgggtt cactaacaaa
    13021 cagtggcggt gtaatgatga atgacttttc ttttacaccg ctgctgtttt caatccacga
    13081 attgaccaga aagcgctcat tggtatggct atttctaatc cataccgcag tctgtttagc
    13141 atcagcggga taaataatac gcgtggcacc taatgcgatc cctccggcag catgggcgct
    13201 ggccgaaatg attaaataaa atataatgag catcaata
//
LOCUS       DAPPNJ010000125        13215 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000125 DAPPNJ010000000
VERSION     DAPPNJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.126"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            161..1660
                     /gene="pitB"
                     /locus_tag="RPM73_003020"
     CDS             161..1660
                     /gene="pitB"
                     /locus_tag="RPM73_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDX6521357.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1709..2401)
                     /locus_tag="RPM73_003021"
     CDS             complement(1709..2401)
                     /locus_tag="RPM73_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDX6521358.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2575..3288
                     /locus_tag="RPM73_003022"
     CDS             2575..3288
                     /locus_tag="RPM73_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDX6521359.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3320..4078
                     /locus_tag="RPM73_003023"
     CDS             3320..4078
                     /locus_tag="RPM73_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521360.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4124..5473
                     /locus_tag="RPM73_003024"
     CDS             4124..5473
                     /locus_tag="RPM73_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDX6521361.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VSKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKP"
     gene            5473..6297
                     /locus_tag="RPM73_003025"
     CDS             5473..6297
                     /locus_tag="RPM73_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521362.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6309..6869
                     /locus_tag="RPM73_003026"
     CDS             6309..6869
                     /locus_tag="RPM73_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDX6521363.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6900..7970
                     /gene="lptF"
                     /locus_tag="RPM73_003027"
     CDS             6900..7970
                     /gene="lptF"
                     /locus_tag="RPM73_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDX6521364.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVYVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7967..9046
                     /gene="lptG"
                     /locus_tag="RPM73_003028"
     CDS             7967..9046
                     /gene="lptG"
                     /locus_tag="RPM73_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDX6521365.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSSGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9081..10253)
                     /locus_tag="RPM73_003029"
                     /pseudo
     CDS             complement(9081..10253)
                     /locus_tag="RPM73_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10253..10501)
                     /locus_tag="RPM73_003030"
     CDS             complement(10253..10501)
                     /locus_tag="RPM73_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX6521366.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10533..11447)
                     /locus_tag="RPM73_003031"
     CDS             complement(10533..11447)
                     /locus_tag="RPM73_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDX6521367.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11444..13174)
                     /locus_tag="RPM73_003032"
     CDS             complement(11444..13174)
                     /locus_tag="RPM73_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDX6521368.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         3174 a         3265 c         3426 g         3350 t
ORIGIN      
        1 gttttgatta tttctaaagg ttttgaatga atgcttattg tctgatacac aagaaataac
       61 actcttttta tcgttaaaaa atgatatttc actttgccca tgccgttaaa gtcactgata
      121 atgcgtccgt tcgtaaattc aaaatggcgt aatctaatat atgctaaatt tatttgttgg
      181 ccttgatata tacacagggc ttttgttatt gcttgctctg gcatttgtgt tgttctacga
      241 agcaatcaat ggttttcatg acacggcgaa tgcggtggca accgttattt atactcgtgc
      301 catgcaacca caacttgctg tggtgatggc ggcatttttt aacttttttg gcgtgttatt
      361 gggcggactt agcgttgcct atgccattgt ccatatgtta ccaaccgatt tgttgctgaa
      421 tatggggtca acccacggtc tggcgatggt cttttccatg ctgctggcgg cgattatctg
      481 gaacctggga acgtggttct tcggtttacc ggcctccagt tcgcacacct tgattggtgc
      541 gattatcggc atcggtttaa ccaacgcgct gttaaccggc tcatcggtga tggatgcgtt
      601 aaacctgcgt gaagtgacca aaattttctc ctcgctgatt gtttccccta tcgtcggcct
      661 ggtcattgcg ggaggcctga tattcctgct gcgacgctac tggagcggga cgaaaaagcg
      721 tgaccgtatt caccgcattc cggaagatcg caaaaagaaa aaaggcaaac gtaaaccgcc
      781 attctggacg cgtattgcgc tgattgtttc cgctgcgggc gtggcgtttt cgcacggcgc
      841 gaacgacgga caaaaaggga tcggcctggt aatgctggta ctggtgggga ttgcccctgc
      901 tggcttcgtc gtcaatatga atgcgtccgg ctatgaaatt acccgtaccc gcgatgccgt
      961 taccaacttc gaacactacc tgcaacagca tcctgaactg ccgcagaagt tgattgcgat
     1021 ggaacctcca ttgcctgcag catcgactga tggcacgcaa gtaacagagt ttcactgtca
     1081 tccggcaaat acctttgatg cgattgcgcg cgttaaaacg atgctgccag gcaatatgga
     1141 aagttacgag ccgttaagcg tgagtcagcg cagccagctg cgccgcatta tgctgtgcat
     1201 ctctgatacc tccgcgaagc tggcgaaact gccaggcgtc agtaaagaag accagaacct
     1261 gctgaaaaaa ctgcgcagcg atatgttaag caccattgag tacgctccgg tgtggatcat
     1321 catggcggta gcactggcgc tcggcattgg caccatgatt ggctggcgtc gtgtagcgat
     1381 gaccatcggt gagaagattg gtaagcgcgg catgacgtat gcgcaaggca tggcggcaca
     1441 aatgacggcg gcagtgtcta tcggccttgc cagttatatt gggatgcctg tctccacaac
     1501 acacgtcctc tcgtctgcgg tagcagggac gatggtggtg gacggcggtg ggttacagcg
     1561 taaaacggta accagcatcc tgatggcgtg ggtgtttact ttaccggcgg caatttttct
     1621 ttctggtggg ctgtactgga tagcattgca gttgatttaa ccttcctgaa aatgcccggt
     1681 cctggcgatc gggcatttcc atttttgact agtgataacc acgcgcggtc ataaaatccg
     1741 taatcgcttt ttctgcatca accaacacct gttccagtgg ccgattggca tcgatatcaa
     1801 ccagttgtgc accaccaaag gttaactgtg gcgttatggc aatcttcctc gccagcgatt
     1861 cccgtttatg gtcgggttta cgtgcacagg caacttcaag gtcaacattg agtttgatga
     1921 ccagatcagg cttatggctc gccatccagt gaaacgcttt acgttcctgg cttgccagcc
     1981 atgagacaaa acgaccacct tcaacgttag gtgggaacac cgtaccatcg taagcgccag
     2041 gaatttggtc ctgaggataa cggtcggtta gaacaattaa cccgcgacga cgacaggcaa
     2101 gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc tgtaattacc agcgccggaa
     2161 ctggtcctgg caattttttt gctgttttca cctgatttcg ttcaattgtt ttatgtaagg
     2221 attttcccat caacggtaat tttgtcactg cacgaccgac atttccggcc tgttttccta
     2281 aatgaactct ttcggcagca ccatattttt cgacaacggt aatgagatgt tcacacaccg
     2341 ttgacttgcc tgaaccatcg ctaccaataa cggcaattaa tggaggtgtt attgactgca
     2401 tgaatatatc cttaaattta aatatccatc caaaatattt atttggttaa tatgttttta
     2461 tgaaagcgta attcaggtca atgtcacaat taaccattgt cacaataagg ttgaacggat
     2521 attctgagca cggaccacgt aatattcaat acattattta ctgccgggtt tttcgtgaat
     2581 caacgtaata tgtcaataat taattccacg ccagtgcatg ttattgccat tgtaggatgt
     2641 gatggttcag gtaaatcgac cctcacggca agcctggtaa atgaactggc agcaagaatg
     2701 ccaacagaac acatttatct cgggcaatcg tccgggcgaa ttggcgaatg gatttcacag
     2761 ctccctgtta ttggcgcacc ttttgggcgt tatctgcgaa gtaaagcggc acatgtgcac
     2821 gaaaagccct caacaccgcc tggcaatatt actgcactgg ttatctatct gctttcctgc
     2881 tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc agcaaggctt tctgctcatc
     2941 accgaccgct acccgcaggt tgaagtgccg gggtttcgct ttgatggccc gcaattggca
     3001 aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc agcgcgagct gaagctgtac
     3061 caatggatgg cgtcttattt gcccgtattg ttgattcgtc ttggcattga tgaacaaacc
     3121 gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac aggaaaaaat cgccgttacg
     3181 ccgcaactga cattcaatgg cgcaaagatc cttgaactgg atggtcggca accagccgat
     3241 gaaattatgc aagcgtcact acgcgcaatt cacgccgcgc tttcctgatc accaaaatgg
     3301 ataggggatg atttaacgaa tggatgcact acaaacacaa actgttaata gtacaaccgc
     3361 accgcagccc aactacattc cggggctgat tgcggtggtc gggtgtgatg gcaccggtaa
     3421 atccacgctg accaccgacc tggtgaaatc gctgcaacaa cactggcaaa ccgagcgccg
     3481 ctatttgggg ctgctctccg gcgaagacgg cgacaaaatc aaacgattgc cgttggttgg
     3541 cgtctggctg gaacggcgac tggcggccaa atcctcgaaa acccaaagca tgaaaaccaa
     3601 atctccggcg ctatgggcgg cggtgattat gtactgcttc tcgctgcgaa gaatggcgaa
     3661 tctgcgcaag gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg atcgcttccc
     3721 gcaggctgaa atttcgggct tttattatga cggaccgggg attggcgtcg aacgtgcgac
     3781 cgggaaaatc agcatgtttc tggcgcagcg cgaacggcgt ttataccaac aaatggcgca
     3841 atatcgcccg gaattaatta ttcgcctggg cattgatatt gagactgcca tctcccgcaa
     3901 gcctgaccat gactatgccg agctgcagga caaaatcggc gtcatgtcga cgattggcta
     3961 taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag tgctggaaca
     4021 ggcacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt taacttaagg
     4081 ctggcgggat actttttact atcaacgata aggtcaggct gaattggcgg gttttaacat
     4141 caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg cctggttagg
     4201 ctccagtaat gtcgtgagcg ccttgctggg gctgttggcg ctctcgtgtg ccggtaaagg
     4261 gatgacgccc gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca agtcgatcag
     4321 cgattttatt aagtttcaga catggcagct ggtggttcag tacggaacgc cagccttgac
     4381 caacaataat ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc tggatatcgt
     4441 cagcggcgcg gtggcgattg tcggcggcat tgccttactg ccattccttt cccattcatt
     4501 aggtctggat gaccaaagct tttggctggc ggcgctgtat tgcacgctca ttccttcaat
     4561 ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgcttcgatt taattgctgt
     4621 acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct ggtattttga
     4681 ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg aatctggttg gcggcaccat
     4741 gtactggtgg tttgcagcgc gcgaattacg ccgcagaaat atccataatg ccttcaaatt
     4801 gaatctgttt gagtctgccc gacacatcaa aggcgcgtgg agttttgtct ggtcaaccaa
     4861 cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg tggggatcgt
     4921 gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg acgccgccgg
     4981 aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt tagatccgcg
     5041 caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg gaatcggtat
     5101 tctggtggcg ctcgcggtgt ttatcgtcag caagccgctc atttcgctgg tgtttggcgt
     5161 taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg tgatttcgat
     5221 gctgggcttc cctcaggagt cattgctatt gatggcggga aaacagcgcg cttttctcgt
     5281 ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct gtcatctgtt
     5341 tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggacg tggcgctctc
     5401 gttaattcca accttaaagg cattttttca gcgccattcc ttgctttata acgcagccgg
     5461 agagaaaccc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg tctatttgtt
     5521 gcgattgaag aagatgatgt cgttgatgca caggttagtt tgccgccgac tattgcacta
     5581 agttgtacca ccgaaatcat tcatgataat tacgcgttat gcctgcaatt ctggctcaat
     5641 ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac aagccaaagg cgatgagctg
     5701 actgctgatg agcgcaaaca atttaaatac atgcgagcgc gctacaagca cctgcgtttt
     5761 gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa aacgaccgtt
     5821 tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt gtcgtattac
     5881 ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa ctattcatta
     5941 cgccatagcc agttagaaag cgtaagcagt tttattgcct atcggcaaaa gcaaatgcat
     6001 accctgaaag agattattgc caaaccgcga ctaaccggca gggaatttca tgatgtgcga
     6061 aaaatcatca gccaacaggt ttcttattac gacaccttaa gatcgcttga tcctgaaaat
     6121 aaagaggcgt tacagatttc ccgtttcctc gccgccatta atggcctgat gggagataaa
     6181 cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtatgatgc accgttggcg
     6241 ctggatagcg atattcgtca gcgcttagag ctgctgattt cgcgttttcc gctatagccg
     6301 gagcaatgat gtcatctcgt ttgattattg cgttgattat catgctctta gccccgggtg
     6361 tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc caggaaggcg
     6421 gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac agcacacaat
     6481 tagcgggaaa agtccgcatt attcagtata tcgccgggcg taagtcggcg aagaaaaaga
     6541 actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt tttcagccca
     6601 ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt gttggcaaaa
     6661 tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcgt tattgacggc aatggacagg
     6721 gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg aataaagccg
     6781 ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat catgtcattg
     6841 cgctggcgca gaaactgatc aatgaataaa aaattcaaag gattgagggc gttagtgaga
     6901 tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg attatcgtcg
     6961 gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc gaagcggcaa
     7021 atgggacatt agctcttgat gttgtgctgg atatcgtttt ctacaaagtg ctgattgcac
     7081 tggagatgtt gttacctgtt ggactgtatg tgtcagttgg cgtgacgcta gggcagatgt
     7141 ataccgattc ggaaattacc gctatctctg cggcgggggg cagtccggga cgcttgtaca
     7201 aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg tcgatgtatg
     7261 gtcgtccgtg ggcttatgcg cagatttatc aactggagca gcagtcgcag tcggagctgg
     7321 atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga atgatccttt
     7381 cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat acttctactg
     7441 ccaatcgaac ccgcattttc cgcgcccgtt cggtttatgt ggttgaccca tcacccgaga
     7501 aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcag gggcgtgacg
     7561 acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggtt caaagcccta
     7621 acgtcaaacg caaagcgaaa tcggtcacgg agctggcgcg ctccgtcttt cctgccgatc
     7681 atgccgaact gcaatggcga caaagccgtg gtctgacagc attgttgatg gcgctgctgg
     7741 ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacgtta ttgccactga
     7801 cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacgctg gtggctaacg
     7861 gcgcgattcc cctcattcct ggtttgtggt tagtgcccgg actcatgcta atgggcctgc
     7921 tgatgctggt cgcacgcgac ttctctttgc tgcagaaatt ttcccgatga atgttttcag
     7981 tcgctattta atccgtcatc tctttctcgg ttttgccgcc gccgcagggc tattgctgcc
     8041 gctttttacc accttcaact tgattaacga actggatgat gtcagctcgg gcggttatcg
     8101 ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg tcgaactttc
     8161 gccgtttatc gccttattgg gagggattgt tggcctgggg cagttatcga aaaacagtga
     8221 gcttaccgcc attcgcagca cggggttttc tatcttccgt attgcactgg tggcgctggt
     8281 tgccgggata ttgtggactg tttcgttagg cgcgatagat gagtgggtgg cgtcgccatt
     8341 gcagcaacag gcgctgcaaa tcaaatcgac cgccaccgcg ttgggggagg acgatgacat
     8401 taccggcaat atgctgtggg ccaggcgcgg caatgaattt gtgacggtga aatcgctgaa
     8461 tgaacagggc cagcctgtgg gcgtggagat ttttcattat cgtgacgatc tctcgctcga
     8521 atcctacatt tatgcacgca gtgccaccat taaagacgac aaaacgtgga tcctgcatgg
     8581 tgtgaatcat aaaaaatggc ttaacggtaa agaaacgctg gaaacatcag ataatcttgc
     8641 ctggcaatcg gccttcacca gtatggatct tgatgagtta tcgatgccgg ggaatacttt
     8701 ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc aacccagcag
     8761 cgaataccgc cttgcactgt gggaaaaact ggggcaaccg atcctgaccc tggcgatgat
     8821 tttgctggct gtgccgttta cctttagcgc cccgcgctcg ccagggatgg gtagccgtct
     8881 cgctgtaggc gtcatcgttg gcttactcac ctggatcagc tatcaaatca tggtcaattt
     8941 gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg tagcgtttgt
     9001 gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc gccgtggcgc
     9061 aacaccacgg cgggctcaca ttactgcttc agagtagcaa atgcctgaat gatggcatcg
     9121 atttgctcac gactgtgggc agcgtttacg ctacagcgca gcagcgtgat gcctgcaggc
     9181 gcggcaggtg gcaggataag attcacataa acgcccagcg aaataagctc gcgccagatg
     9241 cgtaaacctt cctctttcga accgattatt accggcacta ccgggctaat atgtgaactt
     9301 aactcataac ccagtttttg cagaccgtca tacagatggt tggcgttatc catcagcttt
     9361 tgccgcagct ctggttgtgt ggcaatagtt cttaacgacg agcgcacggt cgcaatggag
     9421 gacggagaag gagaggcggt aaaaatatac gggcggctgc cgtagcgcag cacttccatc
     9481 gcttccgaac cgacagcaaa accgccaatc taggccagac ttttactgaa ggtgccaacg
     9541 ataatatcca catcatcttc aacgccgaca gcttcagcca gtccgcgccc cgttgcgcct
     9601 aacacgccaa acgaatgcgc ttcatcgaca atcaaataac caccgagacg acgtttgata
     9661 tcaacaattt ccgccagcgg cgcaacgtca ccgagcatgc tgtagatgcc ctcaacgatg
     9721 ataatcgcct ctttcgcccg ttcaccgagg cgaaccatgc gccgttccag atctttagca
     9781 tcgttatggc ggaaacgaat aatttccgcg ccaccgagag aacaggcgtc ataaatactg
     9841 gcgtggctgt cggcatccag taacaccacg gcgttatggt cggcaagtgc gctgatgacg
     9901 cctaaattag cggtataacc ggttgaaaaa acgatagcgg tagggcgctt gaaaaactcg
     9961 gcaatttctt tctctaacgc caggtgcggc gcataactgc cgtttgccat acgagaaccg
    10021 gtggtgccgg tgccttgggc cgctaacgct gcctgacctt cgctgatggc atcatgatta
    10081 aaggttaaac cgagataatt attggtcccg gccagaatca cttttttatt gccgatccgg
    10141 ccttcggtgg cggaatacac ttcatcaata caggtgccaa acggtgttaa cccgttgtca
    10201 ctaaattgca gacgctcgcc agcaaggcgc gcatatttat cgtatagccc cattatttat
    10261 tctccagcca tggaagtaga gtttccatta attgcgcagg ggttttgaca tccagcagaa
    10321 tattaatagg aatggaaata tcgaatctgt cttccagcat cattaagaga tccatcactt
    10381 tgatcgactc caggccaaga tcgttgacga gatcgctgtc gggcttaatt tccacaccgt
    10441 tttcgaccaa atcctgtaaa caggaaagga tgtaatccat tactatttca cgatttacca
    10501 tagaaacgtt aacacacctt taaacaagac aatcaaaata gcccgttgcg taatgcttgt
    10561 tccaggctaa tcccgggaaa ccaattaata tcttcagaaa tacggttatt acttgccgac
    10621 cagtcggcgt gtgttaattc acgaattttc gagcgggtca gcataggttc tttacctgcc
    10681 aggcgactca acgcggtact gatatccgca aggcaggtga gtaccggcag aggaatacca
    10741 accattcgca cggaaccgca acggacgttg gcggcaagtt gctgtatgcg ttgccagtca
    10801 tagccgccag cgacgccatc gcataattca taggtttgcg tctgtacagt ttcggcgctt
    10861 aaccactgac ccactgcttg cgcaaaatcg gtgacgtgca ggaaagagag ctgtgtatct
    10921 ggtgcaccaa gtcgtggcag caggccgcgc agcatccagt caaacagcgg ttttaactct
    10981 ttatcgccgg gaccatacac ggctgtcggg cgaaaaacgc ccagcgtaat ttcgtcagcc
    11041 attgcagtca gccgttgttc ggcgatgtgt ttggaatttg cgtaccagga gagctcggga
    11101 tggcgcgccg ccagcgaaga gataaacaga aaacgttggc aaaagcagct ctcttttgct
    11161 gcttgcatca ggcgcaggct gccgtcaacg ttacagcggg tgaaaatctc ttctttgtgc
    11221 ccgcgcactt gcccggcgca atggaccact gcgctggctc cggcaaccag ctcgctaagt
    11281 gaatgagtat cttccagcga accgcgcacc caggtgagat tatcattgac gtgagcgcgg
    11341 gcagtacgcg tcaatgcgcg aacgtgaaag ccgcgggcga gcaggttatc gataatatat
    11401 ttaccgataa acccggtagc gcccgtcacc gcgacagttt gattcatgcc agggattcct
    11461 gcacatgaag gcttgcagca taagccttct gataacgttt tttcgcttcc gcacgggcag
    11521 gcttgccgga ggatgttcgg ggaatactgt ggggcggcaa cagatcgata gccgcggtta
    11581 cgccaaattc gctttggatc cgtgccgcca gcgcgtggat aagctgcccg cgacgttctt
    11641 cgtcgctgat ccgacactgg atctgcaaaa tgattttttc ctggacggta acaaaagcaa
    11701 tcgcatcgcc agaatgaatt tccggttctt gttccgcaat atattcaata tcctgcggcc
    11761 agatattacg gccacgaata ataatcagat ctttaatacg tccggtgaca tacagataac
    11821 cgtccagcag ataaccgagg tcgccggtgt ctaaccagcc cgtcgcggca atctcgtctt
    11881 gcgaaacctg gtcgccaaag taaccgctca tcaggctggg gccggagatg caaatatggc
    11941 ctaccacacg ttccgcgacc ggcatacctg cttcattgcg gatttcaatg ccatgttccg
    12001 gcaacgcttt gccgcagttg acgaaagtcg atacggcgcg tgtttcagca cccggcgcga
    12061 cggctttgcc ctgatattct aggatgtcgc gatccacttc gttaaccaca accccagagc
    12121 cttcatcaga gaagctgaca gccagcgcat tttctgccag tccgtagcac ggcatgaacg
    12181 ttttatcgtc aaagttaacc tgacggaaac attcagcgaa ttgatggagt tgttctgcgg
    12241 agatcggctc agcaccaata ccagcgacgc gccagcagga aagatccagt tcagcgagat
    12301 ctttttcatt cacgcggcgt tggcacaatt catagccaaa cggcggcgca acggaaacgg
    12361 tgccgcgatt tttactgatc aatttaagcc attgcaaagg acgcatggca aaatcctgag
    12421 tgcgcaaata atctactgaa agttgcgtgg cgacgggggt caggagaaag ccgaccagtc
    12481 ccatatcatg gtagaaaggc agccaggaga cgcagcggtc gccagggcgt aatttaatgc
    12541 catcgtggct tatagcacgt agattagcca tcacttcgcg atgggtgata atgacgccac
    12601 ggggaaaacg ggtgctgccg gaggtgtact ggaggtaggc gatatcgttc ggaactggac
    12661 gctggagcgc aacatcggct tccggtaatg ccttaaacca ggcgtggctt aaaacatgta
    12721 attcggggtt gttatgcgtc gcggcattga ccagtggcaa ccactcatca ccagtgataa
    12781 tggctgcggg ctggcaactt gccagtaaac cctggagttt ggcgctccag gaatcccgct
    12841 gaccgacgcc cattggaatc gccaacggga cggcgactaa gccagcatac tgacaggcaa
    12901 aaaaagcctc tacaaacccg ctactcgttt cggcaatcag cgccacgcgt tcgccttttt
    12961 tcaggttcag cgataacaac cgcttcgcac cagcttcggc acgcgttttt aacgtctgat
    13021 attccagttg atcttcaagt tggcaacgtc tgtcataaaa attcattccg gcgctactca
    13081 gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc ggcatagcgc ataggtaggg
    13141 aatgcgtaaa gattttatta gacatataca ccacgaaaaa attggatatc aaaaataaaa
    13201 tgacaataat gcggt
//
LOCUS       DAPPNJ010000126        13202 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000126 DAPPNJ010000000
VERSION     DAPPNJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.127"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(103..210)
                     /gene="rprA"
                     /locus_tag="RPM73_003033"
     ncRNA           complement(103..210)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RPM73_003033"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(396..1508)
                     /gene="ydiK"
                     /locus_tag="RPM73_003034"
     CDS             complement(396..1508)
                     /gene="ydiK"
                     /locus_tag="RPM73_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDX6521369.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1897..4953
                     /locus_tag="RPM73_003035"
     CDS             1897..4953
                     /locus_tag="RPM73_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDX6521370.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELYAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEEKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4950..5360
                     /gene="menI"
                     /locus_tag="RPM73_003036"
     CDS             4950..5360
                     /gene="menI"
                     /locus_tag="RPM73_003036"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDX6521371.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5460..5648
                     /locus_tag="RPM73_003037"
     CDS             5460..5648
                     /locus_tag="RPM73_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDX6521372.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6197..6565
                     /gene="sufA"
                     /locus_tag="RPM73_003038"
     CDS             6197..6565
                     /gene="sufA"
                     /locus_tag="RPM73_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDX6521373.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6574..8061
                     /gene="sufB"
                     /locus_tag="RPM73_003039"
     CDS             6574..8061
                     /gene="sufB"
                     /locus_tag="RPM73_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDX6521374.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            8071..8817
                     /gene="sufC"
                     /locus_tag="RPM73_003040"
     CDS             8071..8817
                     /gene="sufC"
                     /locus_tag="RPM73_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDX6521375.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8792..10063
                     /gene="sufD"
                     /locus_tag="RPM73_003041"
     CDS             8792..10063
                     /gene="sufD"
                     /locus_tag="RPM73_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDX6521376.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            10060..11280
                     /gene="sufS"
                     /locus_tag="RPM73_003042"
     CDS             10060..11280
                     /gene="sufS"
                     /locus_tag="RPM73_003042"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDX6521377.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11293..11709
                     /gene="sufE"
                     /locus_tag="RPM73_003043"
     CDS             11293..11709
                     /gene="sufE"
                     /locus_tag="RPM73_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDX6521378.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11858..12862
                     /gene="ldtE"
                     /locus_tag="RPM73_003044"
     CDS             11858..12862
                     /gene="ldtE"
                     /locus_tag="RPM73_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDX6521379.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12926..13162)
                     /gene="lpp"
                     /locus_tag="RPM73_003045"
     CDS             complement(12926..13162)
                     /gene="lpp"
                     /locus_tag="RPM73_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDX6521380.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3322 a         3258 c         3557 g         3065 t
ORIGIN      
        1 ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct tgtgcaacat
       61 tattggaaag agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa aagcccatcg
      121 tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg atttccatgc
      181 ttataaatca atatgttgat ttataaccgt aagctaatag taggcatacg gactttttgc
      241 gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca ggtactacca
      301 gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa ttagcggggg
      361 ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcggttt ctcgatttcg
      421 ccaagttctt cgagaatttc ttccggttgg tccgtcggcg gcgggacttc ttccacccac
      481 gcggcaaaca gacgccagga aacggctaac agaaccggac caataaacag accgatcatc
      541 ccgaaagcaa tcaaaccacc aataacacca gagaggatca ggatcagcgg taaatcggca
      601 cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac accgctccac
      661 actaacaata ccgttcccca ggtggtatcg ccagtccagt agagccagat aatcgccgga
      721 atcagtaccg gcaacgggcc aagctggaca aggcaggaga ggatcattaa caccgttagc
      781 aacgttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac tgcctgtact
      841 aacgccgtca ccaccacacc cagcgccacc gcgcggatag cctgtgccgc cagcagtacg
      901 gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc ttgtgccacc
      961 tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca atgcaccata
     1021 aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg tggtggtgcc aatataaggg
     1081 cggactttcg ccatgatcgc cgtccccccc atatccagca agttgtgcca gcctgcatac
     1141 agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa cgtcatgtcg
     1201 ccgctggaaa tggctttaat tagcgggccg ctgccgtcga cgatactgtt aaccagcaaa
     1261 gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac ggcgagagag
     1321 cggcggccaa acatgatctt ttgcaaacgt aacaataccg gccaggtggc gataaccacc
     1381 gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca tgccacaatc
     1441 atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg ctgacgaaca
     1501 tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg
     1561 cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggcc gattctgccc
     1621 ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac aactcaggca acacgcaaac
     1681 gatttactcg tcgtatttca agttgcatgt gctgcggcta cgttcgttca ccccagtcac
     1741 ttactattgt aagctcctgg agttcactcg cttgccgcct tcctgcaact cgaaatccat
     1801 agagtaaata aacatcacct ttcagtacgc tattgcagcc gcctgaaaga atagtgtatc
     1861 gacctacatc acagaccgca ggaaagggtc aatataatga ttccacagat ttcccaggca
     1921 cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc tggagcaaca aggttttacc
     1981 ggcgatacgg cgacaagtta tgccgatcgt ctgacaatgt cgaccgacaa cagtatttac
     2041 caacttctcc ccgatgcggt ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc
     2101 cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta ccccccgcgg cggcggcacc
     2161 ggcactaacg gtcaggcgct caaccagggg attattgttg atatgtcccg ccatatgaac
     2221 cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa
     2281 gatcaactca atcagtacct gaaaccgttc ggctactttt ttgcgccgga actttcgacc
     2341 agcaaccggg caacgctcgg tgggatgatc aataccgatg catccggtca gggatcgctg
     2401 gtctatggca aaacgtcaga tcacgtactt ggcgtacgcg cggtgttgtt ggggggcgat
     2461 attctcgata cgcaaccttt acccgtcgaa ttggcggaaa cgctgggtaa atccaatacc
     2521 acaatcgggc gaatttataa cacggtttat caacgttgcc gtcagcaacg ccagttaatc
     2581 atcgacaact tccccaaact taaccgcttt cttaccggtt acgatctgcg tcatgtcttt
     2641 aacgatgaga tgaccgagtt cgacctgacg cgcattctga cgggttcaga agggacgctg
     2701 gcctttatta ccgaagcgcg gctggatatt acgcccttgc ctaaagtgcg ccgtctggtg
     2761 aacgtcaaat atgactcttt tgactccgcg ctgcgtaacg cgccgtttat ggttgaggcg
     2821 cgggcgcttt cggtagagac ggtggactca aaagtgctga atctggcgcg ggaagatatt
     2881 gtctggcatt ccgtcagcga gttgattacc gatgtgcctg acaaagagat gctcgggctg
     2941 aacattgtgg agtttgctgg tgatgatgag gcgctgattg atgagcgggt aaatgcactc
     3001 tgtgcgcggt tggatgagct gatcgccagt catcaagcag gtgtcatcgg ctggcaggtg
     3061 tgccgtgagc tggcgggggt tgaacgtatc tatgcaatgc gcaaaaaagc cgttggtctg
     3121 cttggcaatg ccaaaggtgc cgctaagcca attccgtttg ctgaggatac ctgcgtaccg
     3181 ccggaacatc tggccgatta tattgctgaa tttcgcgcgc tgctcgacag ccacggctta
     3241 agctacggta tgttcggtca cgtcgacgca ggtgtcttgc acgtccgtcc ggcactggat
     3301 atgtgcgatc ctcaacaaga gattttgatg aagcaaattt ctgatgacgt ggtggcgctg
     3361 actgcgaaat acggtggttt gttgtggggc gagcacggca aaggttttcg cgctgaatac
     3421 agcccggcgt ttttcggtga ggaactttat gcagaactgc gcaaagtgaa agcggcattt
     3481 gacccgcata accgactcaa cccagggaag atttgcccgc cagaaggtct cgatgcgccg
     3541 atgatgaaag tggacgcggt gaagcgcggt acattcgatc ggcagatccc cattgcggta
     3601 cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt caactttgat
     3661 gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga accggattca ttcaccgaaa
     3721 gggcgcgcaa cgctggtgcg tgaatggctg cgtttgttgg cggatcgcgg cgttgatcca
     3781 ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt tgcggacgtt aattgcccgc
     3841 acgcgcaata gctggcatgc gaataaaggc gaatatgact tctcacacga agtcaaagag
     3901 gcgatgtcgg gctgtctggc ctgtaaagct tgttcgaccc agtgccccat caaaattgat
     3961 gtgccggagt ttcgctctcg tttcctgcag ctctatcata cccgttattt acgtccgctg
     4021 cgcgaccacc tcgtcgctac ggtcgagagc tacgcgccgc tgatggcacg cgcgccgaaa
     4081 acctttaact tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa acatatcggc
     4141 atggttgatt tgccactgct gtcggtcccc tcgctacaac aacaaatggt ggggcatcgc
     4201 tcggcaaaca tgacgctgga acagcttgaa gctctcaatg cagagcagaa agcgcgcacg
     4261 gtgttggtgg tgcaggaccc ctttaccagc tattacgatg cgcaagtggt ggcggatttt
     4321 gtccgtctgg ttgaaaaatt aggtttccag ccggtgttac tgccattttc gccaaatggc
     4381 aaagcccagc atattaaagg ttttcttaat cgttttgcga agacggcgaa aaagacggcg
     4441 gatttcctca accgtatggc gaagctgggg atgccaatgg tgggcgtcga tccggcgctg
     4501 gtactttgtt atcgcgatga atataaactg gcgctgggcg aggaacgtgg cgagtttaac
     4561 gtcttactgg cgaatgaatg gctggcaagc gcacttgagt cacagccagt ggctacagtc
     4621 agcggtgaat catggtattt ctttggtcac tgtaccgagg ttaccgcctt gccgggcgcg
     4681 ccagcacaat gggccgcgat atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg
     4741 ggttgctgcg gcatggcagg gacttacgga catgaagaga aaaaccataa aaattcgctc
     4801 gggatctatg agttatcctg gcatcaggct atgcagcgac tgccgcgtaa ccgctgtctg
     4861 gcgaccggat attcctgccg tagccaggtc aaacgggttg aaggcacggg ggtacgccat
     4921 cctgtgcagg ctttactgga gattattaaa tgatatggaa acgaaaaatc accctggaag
     4981 cactgaatgc tatgggggaa ggaaacatgg tggggttgct ggatattcgc tttgaacata
     5041 ttggtgatga cacccttgaa gcgacaatgc cagtagactc gcggacaaag cagcctttcg
     5101 ggttgctgca tggaggcgca tccgtggtgc tggccgaaag tatcggttcc gttgccggtt
     5161 atttatgtac tgaaggtgag caaaaagtgg ttggcctgga aatcaatgct aaccacgtcc
     5221 gctcggcacg agaagggcgg gtgcgcggcg tatgcaaacc gttgcatctc ggttcgcgtc
     5281 accaggtctg gcagattgaa atcttcgatg agaaagggcg tttgtgctgt tcgtcacgat
     5341 tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt gacgcatatt gcttttggtc
     5401 attatctgca cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc gaggatgcta
     5461 tgtctactca acttgatccc acccagctgg ccattgaatt tttacgccgt gaccagagca
     5521 atctttcacc tgcccaatat ctcaaacgtc tgaaacaact agaacttgaa tttgctgacc
     5581 tgcttactct ctcttctgct gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg
     5641 tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg acctggatta acctccgtcc
     5701 aggttcgatg accttcttaa tactgcttat ctacccgcct gatattgttc aaaaataata
     5761 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5821 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5881 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5941 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     6001 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     6061 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     6121 gtttgctggg ttgaactgat aatcattatc actaacatgc tgttatacgc tgaaagcgat
     6181 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     6241 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6301 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6361 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6421 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6481 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6541 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6601 gatgtcaaaa catggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6661 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6721 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6781 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagc
     6841 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6901 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6961 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     7021 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     7081 ggtgaggcga tccacgatca cccggaactg gtgcgtaaat atctcggcac cgtggtgccg
     7141 gggaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     7201 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     7261 gaaaaaactg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7321 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcagtggtg
     7381 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7441 ggcgataaca acaccggcgg tattctcaac ttcgtcacca agcgtgcttt gtgcgaaggc
     7501 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7561 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7621 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7681 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7741 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7801 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7861 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7921 caacgcggga tcagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7981 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     8041 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     8101 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     8161 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     8221 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     8281 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8341 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8401 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8461 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8521 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8581 gagtcggact ccgggctgga tattgacgca ttaaaagtgg tcgccgatgg cgtgaactcg
     8641 ctgcgtgatg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8701 atcaagcctg attacgttca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8761 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8821 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8881 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8941 atatacgccg ctggaagggc tgaccaatag ccagtttgtc agcattgcgg gagagatatc
     9001 cccacagcag cgagatgcct tagcgttaac gttagacgct gtgcggctgg tatttgtcga
     9061 tggacgttac gtgtcagcac tgagcgatgc gactgaaggc agcgggtatg aagtgagcat
     9121 taacgacgac cgtcaggggg tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     9181 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     9241 gccattgctg ttaatgcata ttacccaggg cgtggcaggt gaagaggtca acactgccca
     9301 ttaccgacat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9361 cagccttaat gatgctcgtc acttcaccgg cgcacggttc actatcaatg tcgcagcgaa
     9421 cgcccacttg cagcatatca agctggcgtt tgaaaacccg gtcagtcacc actttgccca
     9481 taacgatttg ttgctggctg acgatgccac cgcatttagc catagtttcc tgctgggtgg
     9541 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9601 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9661 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9721 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9781 gaccaacaat aatctgctga tgggcaaact ggcggaagtg gatacaaaac cgcagctgga
     9841 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9901 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9961 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
    10021 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
    10081 gtgcgggccg actttccggt gctttcgcgt gaggtaaacg gtttgccgct ggcttatctc
    10141 gacagcgccg ccagtgcaca gaaaccgagc caggtgattg acgccgaggc cgagttttat
    10201 cgtcatggct acgcggcggt gcatcgcggt attcatacct taagcgctca ggcgaccgag
    10261 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10321 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ctggggcaac
    10381 agcaatgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10441 gtcccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10501 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10561 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10621 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10681 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10741 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10801 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10861 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat cattggtctt
    10921 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata gcatagccga gtatgaacag
    10981 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    11041 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    11101 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    11161 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    11221 catgaagaag tggatcgtct ggtgaccgga ctgcaacgta ttcaccgtct gctgggataa
    11281 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11341 tgcgccaatt gggaagagaa atatctctac attattgagc tgggccagcg tctgccagaa
    11401 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11461 attgtcatgc gccagaatgc gcagggaatt attgaattgc agggcgacag cgatgcggcg
    11521 attgtgaaag ggcttattgc ggtcgtattt attctctacg atcagatgac gccgcaggat
    11581 attgtcaatt tcgatgtgcg tccgtggttt gaaaaaatgg cgctcaccca acatctcacc
    11641 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11701 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11761 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11821 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11881 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11941 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    12001 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    12061 caataacact atcgccccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    12121 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    12181 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    12241 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12301 gcctacggca ggcattcgtc aacgttcgct ggagcgtggc attaaattac cgccagtcgt
    12361 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12421 cgaatacctc attcatggta ccagtgcgcc ggacagcgtc ggtttgcgcg tcagttcagg
    12481 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12541 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12601 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12661 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12721 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12781 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12841 tatgttacgc gcgacgcagt agcggtaaac ggcagacaaa aaaaatggcg cacaatgtgc
    12901 gccatttttc acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac
    12961 gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca
    13021 cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat
    13081 cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca
    13141 gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc
    13201 gt
//
LOCUS       DAPPNJ010000127        13196 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000127 DAPPNJ010000000
VERSION     DAPPNJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.128"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            6..233
                     /locus_tag="RPM73_003046"
     CDS             6..233
                     /locus_tag="RPM73_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDX6521381.1"
                     /translation="MLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLLRGW
                     IYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            236..1093
                     /locus_tag="RPM73_003047"
     CDS             236..1093
                     /locus_tag="RPM73_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX6521382.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLSHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1093..3105
                     /locus_tag="RPM73_003048"
     CDS             1093..3105
                     /locus_tag="RPM73_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX6521383.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3106..3627)
                     /gene="sodC"
                     /locus_tag="RPM73_003049"
     CDS             complement(3106..3627)
                     /gene="sodC"
                     /locus_tag="RPM73_003049"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDX6521384.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3708..4604)
                     /locus_tag="RPM73_003050"
     CDS             complement(3708..4604)
                     /locus_tag="RPM73_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDX6521385.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4653..4892)
                     /locus_tag="RPM73_003051"
     CDS             complement(4653..4892)
                     /locus_tag="RPM73_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDX6521386.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4995..5594
                     /gene="nemR"
                     /locus_tag="RPM73_003052"
     CDS             4995..5594
                     /gene="nemR"
                     /locus_tag="RPM73_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDX6521387.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5631..6728
                     /gene="nemA"
                     /locus_tag="RPM73_003053"
     CDS             5631..6728
                     /gene="nemA"
                     /locus_tag="RPM73_003053"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDX6521388.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6809..7216
                     /gene="gloA"
                     /locus_tag="RPM73_003054"
     CDS             6809..7216
                     /gene="gloA"
                     /locus_tag="RPM73_003054"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDX6521389.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7319..7966
                     /gene="rnt"
                     /locus_tag="RPM73_003055"
     CDS             7319..7966
                     /gene="rnt"
                     /locus_tag="RPM73_003055"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDX6521390.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            8059..12675
                     /locus_tag="RPM73_003056"
     CDS             8059..12675
                     /locus_tag="RPM73_003056"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDX6521391.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12726..13073)
                     /gene="grxD"
                     /locus_tag="RPM73_003057"
     CDS             complement(12726..13073)
                     /gene="grxD"
                     /locus_tag="RPM73_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDX6521392.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT         2961 a         3544 c         3528 g         3163 t
ORIGIN      
        1 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
       61 attttcctcc gtttttcaaa gccttcgcgt ttggattcgt catctggctt gtcgtacacc
      121 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
      181 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
      241 aattaaaaca attaagtatt tctcaacaat cattgtagcg atagttgcgg ttcttgccgg
      301 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
      361 agaaaaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
      421 caaccaactg gttaacgcgg gggatcttct gctcacgatc gataaaacgc cctttcagat
      481 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
      541 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
      601 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
      661 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggttgggt
      721 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tgtttgccct
      781 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgagccatat
      841 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacggggtca
      901 cgtttccagt atcggtcggg cgatttatga tcaaagcgtt gaaagtgact caagccttat
      961 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     1021 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     1081 cgtaggtcaa taatgaacgc atcgtcatgg tccttgcgca atttgccctg gttcaaggcc
     1141 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     1201 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     1261 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     1321 ctgggagcca ttgcggcact gcttcttgcc gggcatacgc tcaatgagcc gtggtttttt
     1381 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     1441 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     1501 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     1561 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     1621 acagcccttt taaccgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     1681 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     1741 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     1801 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     1861 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     1921 gaacaattgc tgacggcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     1981 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     2041 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     2101 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     2161 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     2221 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     2281 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     2341 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     2401 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     2461 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     2521 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgataa tctggcaaaa
     2581 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     2641 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     2701 catccaacac tgagtgaaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     2761 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     2821 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     2881 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     2941 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     3001 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     3061 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cttaattact taattacacc
     3121 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     3181 gccaacgtgg accatcagcg ctttgtcttt gatttcatcc agtgatttca gacgaggcgc
     3241 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     3301 atgcccggca ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     3361 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     3421 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggcg aaaactccag
     3481 acctttatcg gtttcagtaa tggtgacgct accaattgac tgccctaccc cttgcgacgt
     3541 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggttgcaa caaccagcgc
     3601 cagaatagcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
     3661 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
     3721 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
     3781 gcttcgacag ctgcccgtac gcgctcaatt ttacctgaac cgataattgg cagcggctgc
     3841 gatggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
     3901 tctgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
     3961 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
     4021 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
     4081 gtaaacggca gacgtgattg caacagggca aattgcgcag gcgtaaagtt cgatacgcca
     4141 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
     4201 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
     4261 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
     4321 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
     4381 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
     4441 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
     4501 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
     4561 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcac ttcctcttat
     4621 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
     4681 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
     4741 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcacgacta
     4801 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
     4861 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcagataa gattattacc
     4921 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
     4981 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
     5041 cgggcgagca actttgcctg caacgtggat tcaccgggat ggggctaagc gaattactaa
     5101 aaaccgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
     5161 gcgttgccat gcttgagcgt cattacgccg catatcacca gcgactgact gagttgctgc
     5221 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
     5281 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
     5341 gcgatctgtc agaagatatg cgcagcgcga tggataaagg tgctcgcggc gtgatcgccc
     5401 tgctctctca ggcgctggaa aatggccgtg agaaccattg tttaaccttt tgtggcgaac
     5461 cgctacaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
     5521 tttcgcgcag tttcgagcca ctggaaaacg cgctggccca tgtaaaaaac attattgcga
     5581 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
     5641 aaaaactgta ttccccactg aaagtgggcg cgatcacggc ggcaaaccgt atttttatgg
     5701 caccgctgac gcgtctgcgc agtattgaac cgggtgacat tcctaccccg ttgatggcgg
     5761 aatactatcg ccaacgtgcc agtgccggtt tgattattag tgaagccacg caaatttctg
     5821 cccaggcaaa aggatatgca ggtgcgcctg gcatccatag tccggagcaa attgccgcat
     5881 ggaaaaaaat caccgctggc gttcatgctg aaaatggtca tatggccgtg cagctgtggc
     5941 acaccggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
     6001 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
     6061 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
     6121 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
     6181 ctcacggtta tttgctgcat cagttccttt ctccttcttc aaaccatcgt accgatcagt
     6241 acggcggcag cgtggaaaat cgcgcacgtc tggtactgga agtggtcgat gccgggattg
     6301 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
     6361 cggataacgg cgcgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
     6421 gcggcattgc ttatctgcat atgtcagaac cagattgggc ggggggtgaa ccgtatactg
     6481 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
     6541 acacagtaga aaaagctgaa acgctgatcg gcaaagggtt aattgatgcg gtggcatttg
     6601 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaaa gctgagctta
     6661 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
     6721 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
     6781 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
     6841 cgatttgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
     6901 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
     6961 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
     7021 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
     7081 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
     7141 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagaaaaag acgccggtcg
     7201 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
     7261 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
     7321 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
     7381 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
     7441 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
     7501 cgtcgaacca tttgtcggcg caaatttgca accagaagcc ctcgccttca acggcattga
     7561 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
     7621 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
     7681 cgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcgc tgaaacgtaa
     7741 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
     7801 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
     7861 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
     7921 gaaacgtctg ggaggctggc cgctacccac cgccgaagag gtgtaatcga gtcgatgcct
     7981 gatgacatat aatgattcag gcatctatag tgaggctatt ccacgcatcc tgcatgatat
     8041 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
     8101 ttttcaccgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
     8161 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
     8221 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
     8281 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
     8341 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
     8401 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
     8461 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
     8521 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
     8581 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
     8641 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
     8701 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcgatcg cccggttacg
     8761 gtagtcaacc cgcccgcaat gcgtcatccg cagatacgga ttgtcgtacc ggtcgccaat
     8821 atggatgatg tctcatcggt cgccagcggc actggcgaag acagccatgc cggccgggaa
     8881 ggctccatct ggccatatat tgaaacgggc atccttgatg aagtgttgcg ccatcgctcg
     8941 accattgtct ttactaattc gcgggggctg gcggaaaaac tgacggcacg attaaatgag
     9001 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
     9061 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
     9121 caccacggct ccgtctctaa agaacaacga gcaatcaccg aacaggcgct gaaatcgggt
     9181 gaattacgct gcgtggtcgc aacctccagt cttgaactgg ggattgatat gggcgcggtg
     9241 gatctggtga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
     9301 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttcccccg tacccggcgt
     9361 gatttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
     9421 acaccaccgc ataatcctct cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
     9481 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
     9541 ctgccaagac gtgtttttga cgccacgctg gatatgcttt ccgggcgcta tccctctggc
     9601 gatttttctg cttttcgccc caaactggtc tggaacaggg agaccgggat attgaccgcc
     9661 cgacctggcg ctcaattgtt ggcggttacc agcggcggca ccattccgga tcgtggcatg
     9721 tatagcgtgt tattacccga aggtgaagaa aaggccggtt cgcggcgggt gggtgaactg
     9781 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
     9841 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
     9901 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
     9961 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    10021 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgagga gcagcgcaac
    10081 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    10141 ggtgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    10201 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtcggt ggtcgccagt
    10261 gatgacggca ttgttgcacg tattcctgac accgatggta aattgcccga tgccgcgatt
    10321 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagttcggca
    10381 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc gggccgcacg
    10441 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    10501 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    10561 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    10621 atatccgatg taacgactac tacgccctcg cctttcgcca ccagtttatt gttcggctat
    10681 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    10741 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    10801 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    10861 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    10921 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    10981 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    11041 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    11101 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    11161 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    11221 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    11281 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    11341 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    11401 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    11461 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    11521 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    11581 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaaactggg tgaagatgac
    11641 ggcctggtgg cactgcatct acaggaatat gctgcagaat cgttcactcc cgccgaagcg
    11701 gatcaggcga atcgttcggc gctgcagcaa gcgatagtcg ctgttctggc tgacggagga
    11761 gcctggtttg cacaacaaat cagccaacgg atacgcgaca aaatcggcga atcggttgat
    11821 ctctctgccc tgcaagaggc gttatgggcg ctggtctggc aaggcgtcat caccagcgac
    11881 atttgggcac cgttacgcgc cctcacccgc agcagttcca acgcacgcac ctcaactcgc
    11941 cgcagtcacc gggctcgtcg tggacgtcct gtctatgcgc aacccgtctc gccgcgggta
    12001 tcttacaaca caccaaatct ggctggacgc tggtcgttat tgcaggtgga gccactaaac
    12061 gataccgaaa ggatgctggc gctggcggaa aatatgctcg accgctacgg catcatcagt
    12121 cgtcaggcag tgatagccga aaatatccct ggcgggtttc catcgatgca aacgctttgt
    12181 cgaagtatgg aagactccgg gcgaattatg cgaggtcgtt ttgtagaagg tctgggtggc
    12241 gcgcaattcg ctgaacgtct gactattgac cgattgcgcg atctggcgac acaagccacg
    12301 caaacgcgcc actatacacc agtggcgctc tctgccaacg atccggctaa tgtgtgggga
    12361 aatcttctgc cctggcctgc acatccggca acgctggttc caacgcgtcg ggcgggtgcg
    12421 ctggtggtcg tttctggcgg caaattgtta ctctatctgg cgcaaggtgg caaaaaaatg
    12481 ctggtctggc aggaaaaaga ggaattactc gccccagagg ttttccacgc gctgactacc
    12541 gcactgcgtc gcgaaccacg gctgcgcttt acgctaacag aagtgaatga tctaccggtc
    12601 cggcaaacgc cgatgtttac gctgctgcgt gaggcgggat tttcaagttc gccacaaggg
    12661 ctggattggg gatagagaaa ggactgacgg atgcccgttc gcatccgtca gtattgcagg
    12721 acggattatt ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc tttgatcagc
    12781 tgctgcagtt cgccacgctg atacatttcg atcacgatat cacaaccgcc gaccagctcg
    12841 ccgtcaaccc acagttgcgg gaaggtcggc cagttagcat atttcggcag ttccgcacga
    12901 atgtccggat tctgcagaat atcaacatag gcaaaacgtt cgccacatgc ggcaagcgcc
    12961 tggactgcct gggcagagaa accgcagctc ggcagtttcg gtgaaccttt catgtacagc
    13021 aggatcgggt tttcagcaat ctggcgttgg attttttcga tagtggtgct cattgtcttg
    13081 cttcctcaaa cttctttacg gcagtaatct gacattgtag cgggtcagtg cggcatcgga
    13141 aaataacatt ttcatcacgc ttttgctatt ttatcccttt gctctatttg ttgcat
//
LOCUS       DAPPNJ010000128        13077 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000128 DAPPNJ010000000
VERSION     DAPPNJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.129"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            13..1665
                     /gene="hcp"
                     /locus_tag="RPM73_003058"
                     /gene_synonym="priS"
     CDS             13..1665
                     /gene="hcp"
                     /locus_tag="RPM73_003058"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDX6521393.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMANLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            1677..2645
                     /gene="hcr"
                     /locus_tag="RPM73_003059"
     CDS             1677..2645
                     /gene="hcr"
                     /locus_tag="RPM73_003059"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDX6521394.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            2778..4496
                     /gene="poxB"
                     /locus_tag="RPM73_003060"
     CDS             2778..4496
                     /gene="poxB"
                     /locus_tag="RPM73_003060"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDX6521395.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPIVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDT
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            4533..5534
                     /gene="ltaE"
                     /locus_tag="RPM73_003061"
     CDS             4533..5534
                     /gene="ltaE"
                     /locus_tag="RPM73_003061"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDX6521396.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            5545..6975
                     /locus_tag="RPM73_003062"
     CDS             5545..6975
                     /locus_tag="RPM73_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDX6521397.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            7074..8087
                     /locus_tag="RPM73_003063"
     CDS             7074..8087
                     /locus_tag="RPM73_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDX6521398.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWVV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(8084..8914)
                     /gene="amiD"
                     /locus_tag="RPM73_003064"
     CDS             complement(8084..8914)
                     /gene="amiD"
                     /locus_tag="RPM73_003064"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDX6521399.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(8911..9234)
                     /locus_tag="RPM73_003065"
     CDS             complement(8911..9234)
                     /locus_tag="RPM73_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDX6521400.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            9360..9875
                     /locus_tag="RPM73_003066"
     CDS             9360..9875
                     /locus_tag="RPM73_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX6521401.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            10093..10821
                     /gene="artP"
                     /locus_tag="RPM73_003067"
     CDS             10093..10821
                     /gene="artP"
                     /locus_tag="RPM73_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDX6521402.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRNLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            10839..11570
                     /gene="artJ"
                     /locus_tag="RPM73_003068"
     CDS             10839..11570
                     /gene="artJ"
                     /locus_tag="RPM73_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6521403.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            11577..12293
                     /gene="artQ"
                     /locus_tag="RPM73_003069"
     CDS             11577..12293
                     /gene="artQ"
                     /locus_tag="RPM73_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDX6521404.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            12293..12961
                     /gene="artM"
                     /locus_tag="RPM73_003070"
     CDS             12293..12961
                     /gene="artM"
                     /locus_tag="RPM73_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDX6521405.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
BASE COUNT         2997 a         3475 c         3549 g         3056 t
ORIGIN      
        1 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcaggaaac
       61 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
      121 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
      181 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
      241 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
      301 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
      361 aacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
      421 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
      481 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
      541 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
      601 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
      661 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
      721 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
      781 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
      841 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
      901 tggcagaatc agcaagtaga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
      961 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     1021 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     1081 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     1141 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     1201 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     1261 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     1321 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     1381 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     1441 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatc
     1501 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     1561 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaatttgg cctgcgttct
     1621 atcaccactg ttgaagaaga catgaagcaa ctgttgagcg cgtaaggagt ttgtcgatga
     1681 cgatgccaac gaatcaatgc ccgtggcgga tgcaggttca tcacattacg caagaaacgc
     1741 cggatgtgtg gacgatttcc ctgatttgcc acgattacta cccatatcgc gccgggcaat
     1801 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     1861 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     1921 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     1981 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     2041 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     2101 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     2161 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgtc
     2221 tcactcgcga actgctggca ggtgtacctg acttagcttc acgtaccgtg atgacctgcg
     2281 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     2341 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     2401 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     2461 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     2521 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     2581 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     2641 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     2701 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     2761 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     2821 cggcaggggt gaaacgcatc tggggagtca caggcgactc tctgaacggt cttagtgaca
     2881 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccacgaagaa gtggcggcct
     2941 ttgccgctgg cgctgaagca caacttagcg gagaactggc ggtctgtgcc ggatcgtgcg
     3001 gccccggcaa cctgcactta atcaacggcc tgttcgattg ccaccgcaat cacgttccgg
     3061 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     3121 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     3181 agcagattcc acaagtactg gcgattgcca tgcgcaaagc ggtgcttaac cgtggcgttt
     3241 cggttgtcgt gttaccaggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     3301 actggtatca tgcgccacaa ccaatcgtga cgccggaaga agaagagtta cgcaaactgg
     3361 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     3421 cgcataaaga gttagttgag tttgccggga aaattaaagc gcctattgtt catgccctgc
     3481 gcggtaaaga acatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     3541 gcttctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     3601 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatatcaatc
     3661 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     3721 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggatacag
     3781 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     3841 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     3901 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     3961 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     4021 cgctgggtgc gcaggcgaca gagccagaac gtcaggtggt cgccatgtgc ggcgatggcg
     4081 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccagtgaaaa
     4141 ttgtcgtctt taacaacagc gtgctgggct ttgtggcgat ggagatgaaa gctggtggct
     4201 atttgactga cggcaccgaa ctacacgaca caaactttgc ccgcattgcc gaagcgtgcg
     4261 gcattacggg tatccgtgta gaaaaagcgt ctgaagttga tgaagccctg caacgcgcct
     4321 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagag ttagccattc
     4381 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     4441 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     4501 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     4561 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     4621 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     4681 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     4741 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     4801 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     4861 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     4921 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     4981 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     5041 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     5101 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     5161 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     5221 agtccgggat tctggctgcc gccgggatgt atgccctgaa aaataacgtc gcacggttgc
     5281 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     5341 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     5401 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     5461 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     5521 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     5581 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctggcggcg
     5641 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     5701 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     5761 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca ggtggctctc
     5821 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     5881 caggccccgc cacatgagca gtcggatcat ctgcgtgctc gtcaggctac ggcggacatt
     5941 cttcgtgaag cgaatgtacc tgtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     6001 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     6061 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     6121 gcgttgttag atcatccggc cagcgaacac cgcatcttcg aagccgccgg accagaggtg
     6181 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
     6241 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
     6301 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcggat
     6361 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
     6421 agcacgttga aagaggagga aaaactggtc aactccagcg actggggcta cgacgctcag
     6481 gcctttgccc gctggcgacc ggagtacggt tattttgcca aacaggcggg gtttaccgtt
     6541 aaaacgtccg ccagccttgc tgctttatgg caggtagtga accaaatcgg cggtaaagag
     6601 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
     6661 cataagctgg cgaaaggccg cccggagcgc gaatatttac agactggcga tgcggtagat
     6721 agctggaaag tgattgtcgt tgaaccggaa aaacaactta cgttgttatt tggcatgaaa
     6781 gcgccggggc tgggacgact gtgttttagc ctggaagata aaggcgacta tcgtactatc
     6841 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
     6901 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
     6961 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
     7021 ggggaaatca cggaagaatg cgcacgttat tttctcaaca acagtggatt gatatgaagg
     7081 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
     7141 aaggcatcag cgtgcgagcg accggtcgca acgaggcaat gggcaaattg ctggaaaaaa
     7201 tgggcgcaga gtttgttccg gcggatctga ccgagctggt ttcatcacaa gctaaagtga
     7261 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
     7321 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggttgtcg
     7381 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
     7441 accatcgcga tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
     7501 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
     7561 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
     7621 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
     7681 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
     7741 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
     7801 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
     7861 cctacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
     7921 aagagccgcc gctgacccac tacggcgtct ccaagttgaa ttttgacttt acgctggata
     7981 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggtatcg
     8041 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
     8101 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
     8161 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
     8221 caggtttaac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
     8281 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttaacccg ctgcgcatcc ggccaggcac
     8341 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
     8401 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
     8461 tatctttcgc cagtggaata agcgcctgaa tctgtgccgg ttcaaacggg gcaaaatatt
     8521 tcactccggc cgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
     8581 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
     8641 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggtaccgca gggaccagat
     8701 aatgcgagct gacctgctta tcggtcagtg tcgccagcga gctatcaaaa tcatctgcgg
     8761 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg cgtcgggtat
     8821 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
     8881 gagcggcagc gatcagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
     8941 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
     9001 gacgacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
     9061 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
     9121 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
     9181 gcaatattca acgatggtct ggccttccag agttggggtt gtagaaaatt gcatgatcga
     9241 tctccttgtt tgttaagcct tcgatctcta aagcattatc agactgatac gctattattg
     9301 aaaggatatc attattatcg tagaacgctt tcagagcgat cgcttataag gaaatcatta
     9361 tgcgctacag caaattgaca atgcttatcc cctgcgcact gctcctcagt gcctgcacca
     9421 cagtcactcc agcttataaa gataacggca cacgcagtgg tccttgcgtt gaaggaggcc
     9481 cggataacgt ggcccagcaa ttttatgact accgcatttt gcaccgcagc aacgacatca
     9541 ccgccttgcg cccttatctg agcgacaaac tggcgacact gcttagcgat gcctcccggg
     9601 ataataacca tcgggaacta ctgaccaacg atccattctc cagtcgcaca accttgccag
     9661 agagcgcccg tgttgccagt gcctcaacaa tcccgaatcg tgatgcgcga aatattcctc
     9721 tgcgtgtgga tctaaaacag ggtgatcaga gctggcagga tgaagtgtta atgattcagg
     9781 aaggccagtg ttgggtgatc gacgatgtac gctatttagg cggcagcgtt catgccacgg
     9841 cgggtacgct ccgtcagtcc atcgagaatc gctaacgcct tgatcaacca ataaaccccg
     9901 taaattgtcc ggcatttcgt gaggaatgcc gacatttatg ctcaccgacc accgcccccg
     9961 ttattttgtg ctatgtttat tgaataatgc gctttgcttt taacttttaa agcataaata
    10021 ttgcataatt attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt
    10081 atacgagtgt caatgagtat tcaattaaac ggcattaatt gcttctacgg cgcgcatcag
    10141 gcgctgttcg atatcacgct ggattgccca cagggcgaaa cgctggtgtt acttggcccc
    10201 agcggcgcgg gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccgcgctcc
    10261 ggtacgctca acattgcagg caaccatttc gatttcacca aaacaccctc tgacaaagcg
    10321 attcgcaatt tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat
    10381 ctgaccgtgc agcaaaacct gattgaagcg ccctgccgtg tactggggtt gagtaaagat
    10441 caggcgctgg cccgtgcaga aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat
    10501 cgttacccgc tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg
    10561 atgatggaac cgcaggtact gctgttcgat gaaccgaccg ccgcactgga cccggaaatt
    10621 acggcacaaa tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc
    10681 gtcacccacg aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat
    10741 ggtcatatcg tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt
    10801 aaaaactatc tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg
    10861 cgttaattgc aggttttagt ctttccgcca cagctgccga aaccattcgt tttgctaccg
    10921 aagcctccta tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg
    10981 tcgacctggc acaggcactg tgtaaagaga ttgatgcaac ctgtactttc tctaaccagg
    11041 cgtttgacag cctgatccca agcctgaaat tccgtcgcgt agaagcagtg atggcgggca
    11101 tggatatcac tccggagcgt gaaaagcagg tactgtttac caccccgtac tatgacaact
    11161 ctgccctgtt tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa
    11221 aagtcggcgt tcagaacggg acgacacacc agaaattcat tatggataag cacccggaaa
    11281 tcactaccgt tccgtatgac agctaccaga acgcaaaact ggatctgcaa aacgggcgta
    11341 tcgacggcgt cttcggtgac accgcagtgg tcactgagtg gctgaaagat aacccgaaac
    11401 tggcggcggt gggcgacaaa gtgaccgata aagattactt cggcactggc ctcggcatcg
    11461 cggtacgtca gggcaacact gagctgcagc agaaactcaa cactgcgctg gaaaaagtga
    11521 agaaagatgg cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga
    11581 atgaattttt tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat
    11641 tgattgtcgg gctggcgctg gcgatgttct ttgcggtatg ggagtcggca aaatggcgtc
    11701 ctgtcgcgtg ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg
    11761 tggtgctgtt tatctatttt ggctcctcgc agctgctgct gacgctttcg gatggcttca
    11821 ctatcaatct tgggttcgtg cagatcccag tgcagatgga cattgagaac ttcgacgtta
    11881 gcccgttcct ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa
    11941 cgcttcgggg cgcgttgaaa gcggtgccgg tgggtcagtg ggagtctggt caggcgctgg
    12001 ggctgtcgaa atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc
    12061 tgcctggcct cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga
    12121 ttagtgtgaa tgatttaatg ctgcaaacaa aaagcatcgc tactcgtacc caggaaccat
    12181 ttacctggta cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca
    12241 ttctcaaacg cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga
    12301 gtatttaccc gaactgatga aagggctaca caccagcctg acgctaaccg ttgcctcgct
    12361 gattgtggca ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt
    12421 gctggtgtgg ctggtgcggg gttatatcac gctgtttacc ggtacgccgc tgctggtgca
    12481 gatcttcctg atttattacg ggccgggcca gtttccgact ttgcaggagt atccggcact
    12541 gtggcatttg ttgtcagaac cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc
    12601 ggcgtatacc acgcagctgt tttacggtgc aattcgtgcg atcccagaag gtcagtggca
    12661 gtcctgtagc gccctgggaa tgagcaaaaa agatacgctg gcgatcctgc tgccgtatgc
    12721 ctttaaacgc tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc
    12781 tctggcttac accattacgc tgatggaagt gatgggatac agccagttgt tgtacggacg
    12841 cacctacgat gtaatggtgt tcggtgcggc agggattatt tacctggtcg tcaacggcct
    12901 gctgacgctg atgatgcgtc tgatcgagcg caaagcactg gcattcgaac ggcgaaatta
    12961 atgtagctgc gtacattcgc cgggggggtt tgtaggtctg ataagacgcg ccagcgtcgc
    13021 atcagacatc agcacggtgc ctgtgccgga tgcggcgtgg acgccttatc cggccta
//
LOCUS       DAPPNJ010000129        13072 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000129 DAPPNJ010000000
VERSION     DAPPNJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.130"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..239
                     /locus_tag="RPM73_003071"
     CDS             <1..239
                     /locus_tag="RPM73_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="activator of nucleoside metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDX6521406.1"
                     /translation="LAAFILLSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGL
                     LTKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ"
     gene            complement(310..1560)
                     /locus_tag="RPM73_003072"
     CDS             complement(310..1560)
                     /locus_tag="RPM73_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDX6521407.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1654..2592)
                     /gene="psuG"
                     /locus_tag="RPM73_003073"
     CDS             complement(1654..2592)
                     /gene="psuG"
                     /locus_tag="RPM73_003073"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDX6521408.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(2580..3521)
                     /locus_tag="RPM73_003074"
     CDS             complement(2580..3521)
                     /locus_tag="RPM73_003074"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDX6521409.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(3945..5636)
                     /gene="fruA"
                     /locus_tag="RPM73_003075"
     CDS             complement(3945..5636)
                     /gene="fruA"
                     /locus_tag="RPM73_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDX6521410.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5653..6591)
                     /gene="fruK"
                     /locus_tag="RPM73_003076"
     CDS             complement(5653..6591)
                     /gene="fruK"
                     /locus_tag="RPM73_003076"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDX6521411.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6591..7721)
                     /gene="fruB"
                     /locus_tag="RPM73_003077"
     CDS             complement(6591..7721)
                     /gene="fruB"
                     /locus_tag="RPM73_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDX6521412.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            8089..9270
                     /gene="setB"
                     /locus_tag="RPM73_003078"
     CDS             8089..9270
                     /gene="setB"
                     /locus_tag="RPM73_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDX6521413.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGMLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9267..9521)
                     /locus_tag="RPM73_003079"
     CDS             complement(9267..9521)
                     /locus_tag="RPM73_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDX6521414.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            9676..10248
                     /gene="yeiP"
                     /locus_tag="RPM73_003080"
     CDS             9676..10248
                     /gene="yeiP"
                     /locus_tag="RPM73_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDX6521415.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            10471..11937
                     /locus_tag="RPM73_003081"
     CDS             10471..11937
                     /locus_tag="RPM73_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDX6521416.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            12055..13041
                     /gene="yeiR"
                     /locus_tag="RPM73_003082"
     CDS             12055..13041
                     /gene="yeiR"
                     /locus_tag="RPM73_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDX6521417.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
BASE COUNT         3213 a         3511 c         3167 g         3181 t
ORIGIN      
        1 gcctggcagc atttatatta ctctcgcagg aaggtgatct ttatcacgaa aagcatacgc
       61 aagcggcaga gtatttaggc gtttcttatc gacatctttt atatgttctc gcgcaattca
      121 ttcacgacgg tttattaact aaaagcaaga aagggtatct cattaaaaac agaaagcagt
      181 tgtcaggact ggcgctggag atggacccgg agaataaatt ctccgggatg atgcagtaaa
      241 aattatttgc aatagcgcga ttgccggatg caacgcttaa tacgttttat ccggtctaca
      301 aatcgagcat tacgccagac caataaagaa cccggcaata gtcgcactca tcaggttgga
      361 aagcgttgct gctgccagcg cccgtaaacc aagctgggcg atttctggag cacgttttgg
      421 cgaaatagcc gaaaatgcgc caacgacaac accgatagaa ccaaagtttg caaacccaca
      481 gagggcgaac gagataatgg caatggtctt gacatccagc gtgccgctcg tttgcagata
      541 gggcgagaaa ttgagataag caacaaactc attaatcgcc agtttctgcc caatcaaact
      601 tccggcaaga tttgcatcac tccagtccac ccccataatc catgccaacg gggccagcac
      661 atagccgaaa atgctttcca gagagacatt ggcaaaacca aaccagccgc caactccacc
      721 aataatgccg ttgatcagcg caataattgc cacaaatgcc attaccaccg tcgccacacc
      781 ggcggcgatt ttcagcccgg tcattgcacc gttcgccgca gcttcaataa tgctttttgg
      841 cggtgtttcg ctaaacgaca gattttcaaa cgtaacttgc gaaggttccg tcgccggact
      901 aagaatacgt gcaaacagga taccgccagg aatagccatc agcgaagcgg ctaacaaata
      961 gtcaatcggc acgcccattc cggcatagcc aatcatcatc gaacccgcaa tagacgccat
     1021 gccgctacaa atagccgtaa atagctcgtt gcgattcatg cgatcaataa aaggtttaac
     1081 gatcgccggg atctcatttt gcccgaggaa aatagtggtg actgcgacaa aagattcgat
     1141 tttgctgatg ttgagggctt tctgaaaaat actgccaaga atacgaatca gcagccccat
     1201 tacgccaatg tagtacagca agctgataag cgcggtaacg aaaataatcg ccgggagtac
     1261 gcgaaaggcg aagataaagc ccgcaccgtc aaacagaaca tccattttgg gaccgaccag
     1321 cgaaccaaaa ataaaggcac ttcctgcgtc actgtaggac atcactttat ggacgcctaa
     1381 tgccgcctgc tctactgccc attttcccgg gggaaaatag agcatgatgc caccaatggc
     1441 aatttgcagc agcagcgcag cgccaaccgt gcgcaagctg atgctctttt tatttaccga
     1501 caacacaaag gcaattgcca gcaataccac catccccaca acacttctca ttatatccat
     1561 aatgattttc ccttcatgcc agtaaacccg gcgtcagcgc caggttttgg tatgtttgat
     1621 gagtacgggc gacggctttc tgcccgccag aaattaaccc acgagacgct gatattcttt
     1681 ggcaatttcg ctcgccagaa tggcgttatt aaagaccagt tgaatattgg atttcaggct
     1741 atcaccgcct gttagctcag caacgcgcgc cagcaggaac ggtgtacttt ctttaccaat
     1801 aacgccctgc tcttcggctt cggcgacggc ttgatctatg gctgcattga tagattcctc
     1861 cggcatcgca aactgttccg ggatcgggtt cgctaccacg aggccaccgt tcagcccgct
     1921 ttgccatttc accgccattg cacgggcaat ttcactggcg ctgtcgagac gaatgctgac
     1981 gtcaaacgag ctggtacggc agaaaaacgc aggcagcgct ttagtctgat agccaattaa
     2041 cggcacaccg aaggtttcta aatactcagt ggttaatcct aaatcgagaa tagatttcgc
     2101 cccggcacaa acaacggtga cattagtatt tgccagttct tgcaaatcgg cagaaatatc
     2161 gaaggtatgt tccgccccgc gatgcacacc accaattccc ccggtggcaa atactttaat
     2221 tccggcaagc gccgcaataa tcatcgttga agccacagtg gttgcgccat tttttccggc
     2281 ggcaacaaca aaaggtaaat cgcgacgact aactttggtc acgttatgcc cttcacgacc
     2341 cagtaattca atttcttctt tgcttaaacc cactttcatc acgccgccaa taatggcgat
     2401 agtggcaggt actgcgccct gtttacgaat agtttcttct acttcaattg cggtctgggc
     2461 attttgtggg aacggcatcc cgtgagaaat aatggtcgat tccagcgcca caaccggttt
     2521 tttgtttttt aaagcgtcct gcacttccgg ggaaatttgt aataattcag gggaaatttt
     2581 taattcagac attctgcgtt ctccactaac gatataacgt tggcaatcga taaatcgggg
     2641 ttattggtgt attcacagga gagcgccatt gacgaacatc cctgtgcgaa acgaacagat
     2701 tcggcaaacg gcattccgtc tacccaacac gaagcaagtc ccgccatcat ggcatcgccc
     2761 gctccggtaa cattaataac attggttttg atcggcgcag accagccatt ttcaccgctg
     2821 atatcgctgt aataaacgcc atcgccgccc atgctcaata ccagtcggtt caggccatgt
     2881 tgatggaacc aggcagcaac ttttgccaca tcgtcacgcc ctgacagcgc aatcccactc
     2941 agggtttccg cttcaaggcg gtttggcttg agagtgtgga tctgatttag acggtcgcgc
     3001 actttgacac atttccacgc tgaaaccgga tcgacaaata cgggtacgtt ggcggcatta
     3061 tccagaatcc atgccagtgc ctcttcactg atattacagt ctgcgacaat gacctttgcc
     3121 ctctgaataa attcacggtg ctgtgcgaga tattcagctg taatagcgtt gctaatattc
     3181 atgtcattta tagcaaccag catttcaccg gtattatcga gtaatgataa atagcttgac
     3241 gtattttctc ctggcacaat caggcattta tcgacataaa cgccagattg attggtttgc
     3301 gttagcagcg attgaccata aaagtcactg cctacggcgc tcagtagcca ggctttgttc
     3361 cccagcaacg ccaggttttg tgcaatattg cgcccgactc caccaggcgt aaattttatt
     3421 ttacctggat ttgaatccgc ataatttaat gattcatgtg aatatccggc gacatcaata
     3481 ttcgccgagc ctataattac gacataatcc ttttcgcgca tagcacgtcc tctggtaaaa
     3541 aattaccaac cctgtcaatc cacaaaaata tagatactgc aaagacggtt taaacatgtg
     3601 ttcatattta aacatgtgct catagtatgc atgttttacg taaagtaaat taccgccaga
     3661 gaggcaaaat gggcagattt gagcaggctc acaaaatgcg attaaatgcc tgaattacaa
     3721 aagcgaggaa tttgtgacgg gatgcacaga acaggaacaa taaaaaaggg cagaaaataa
     3781 tctgcccttg aggaaataac cttagtgttt gatgaacgtt tcaagtttct actgaaagat
     3841 aaaaccattt aaattcataa aattatattc acaagctggt cattcccttt gcgctcatcc
     3901 aatgatacag ggcaggaaat ttctgccctg taacacacct tttattacgc tgctttcgct
     3961 actgcgtcca cttccggacg tttcaggaag gcataggcca aacccgccac cagcgtaccg
     4021 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
     4081 aacagaccac cgtgcggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
     4141 agcgccccac ccacgataca gcacggcagc acacgcatcg gatcacgagc agcaaacgga
     4201 attgcacctt ccgaaataaa gcacagtccc agtaccagag cggctttgcc accttcctgc
     4261 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca ggcccattgc cagcggtggc
     4321 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
     4381 acaccgaatg cgtacgctgc tttatttacc ggaccgccca tgtcagtaca catcatgcca
     4441 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
     4501 aacccttcga gaatgccagc aaccggttta ccgatcaggt agatcatcgc cagaccgacc
     4561 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
     4621 agtttcagtt gcgtactgat taacttcgca atgtaaccag ccaggaagcc cgcaataata
     4681 ccgccaatga agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggcgtg
     4741 agacccggac gatcggcaat ggagaaggca atgtaacctg ccagtaccgg caccatcagc
     4801 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
     4861 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacgaccatc
     4921 ggcagcatgt aagagacgcc cgtcagcaag tgacggtatg cgcctgcact ctctttctta
     4981 ccttcagagg tcgccgtttg agctttgccc gccggttcat acggcgttgc ttcagcaacc
     5041 gctttatcca gttcctgcgc ggttttcttc agcgccagac cggtagaggt acgatacatc
     5101 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
     5161 gctgcggcta cttcctcggg agtgattgca ttacccgcgc caacagaacc acgggtttca
     5221 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
     5281 gtgtgtgcta cgccagtcgg gcaagcagtc acagcaacta cgcgtttcgg accgctggcg
     5341 gcaaccggtg ctgtcgcagt gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
     5401 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
     5461 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
     5521 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
     5581 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
     5641 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
     5701 cggacgatcg gtaataccca cattgctttg acttaccgcc agggcggcaa cagctgtcgc
     5761 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
     5821 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
     5881 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
     5941 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
     6001 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
     6061 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
     6121 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
     6181 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
     6241 gtcggcgggg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
     6301 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
     6361 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
     6421 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
     6481 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
     6541 aacaaggtca taagccggat taagggtgat agtagcaaca cgtctgctca ttatgcgccc
     6601 tccccaagac cagcagcgat agcgtcgccg attgctttca gcgcctgttc agcatctgca
     6661 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
     6721 agactacgtc cgtttgccgg tttgccggta ccatcaaggt ttgtcacggt aatatcactg
     6781 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
     6841 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
     6901 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
     6961 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
     7021 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
     7081 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
     7141 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccgc ctctttcaga
     7201 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
     7261 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
     7321 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
     7381 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
     7441 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
     7501 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
     7561 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
     7621 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
     7681 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
     7741 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
     7801 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
     7861 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgccattt ttcctaattg
     7921 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
     7981 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
     8041 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgaccagcat gcataactcc
     8101 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
     8161 tttctcaccg gtattgcggg cgctctgcaa accccgacac tcagtatttt tcttactgat
     8221 gaagtacatg ctcgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
     8281 attctggtaa gtcagtttct cgccgggcgc tctgataagc gcggcgatcg caaatcgctg
     8341 attgtctttt gctgcctgtt aggcatgctg gcctgcaccc tttttgcctg gaatcgcaac
     8401 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
     8461 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
     8521 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
     8581 gccttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
     8641 gtttgcggtg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
     8701 accggcacga tcgaagcgcc gcgccgtaac cgtcgcgata cgctgctgct gtttgtcatt
     8761 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
     8821 aacgaactgc atcttcccga gaaactggcc ggtgtgatga tggggaccgc cgccgggctg
     8881 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
     8941 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
     9001 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
     9061 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
     9121 tataccaaca cgtcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
     9181 gagatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
     9241 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
     9301 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
     9361 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
     9421 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
     9481 ggggcagtgc aacacgcccc gcaacccgga cggcatttca tacgttgttc tcttaaggtt
     9541 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
     9601 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
     9661 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
     9721 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
     9781 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
     9841 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
     9901 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
     9961 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    10021 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    10081 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    10141 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    10201 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    10261 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    10321 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    10381 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    10441 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    10501 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    10561 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    10621 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    10681 cagctccgcg cacagaacca tttatatacc gtgctggaaa aaggtgcgga cggcaatcag
    10741 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    10801 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    10861 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    10921 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    10981 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    11041 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaacgttc gccagaactc
    11101 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    11161 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtgaa tgatccctgc
    11221 gcgattagct gcgaaccgtt tatccagtgg gtggtggaag ataacttcgt cgctgggcgt
    11281 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    11341 aaactgcgga tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    11401 ttcgcccata tcagtgattg catgcaggat cgcgcatttc gccatgccgc cagaacatta
    11461 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    11521 gacaagttaa ttgcacgttt tgctaatccg gcgctgaaac ataagacctg gcaaatcgca
    11581 atggatggta gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    11641 cgcgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    11701 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    11761 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    11821 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    11881 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    11941 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    12001 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    12061 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    12121 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    12181 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    12241 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    12301 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    12361 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
    12421 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    12481 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    12541 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    12601 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    12661 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    12721 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    12781 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    12841 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    12901 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    12961 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    13021 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt ag
//
LOCUS       DAPPNJ010000130        13033 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000130 DAPPNJ010000000
VERSION     DAPPNJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.131"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            273..4733
                     /gene="gltB"
                     /locus_tag="RPM73_003083"
     CDS             273..4733
                     /gene="gltB"
                     /locus_tag="RPM73_003083"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDX6521418.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4746..6164
                     /gene="gltD"
                     /locus_tag="RPM73_003084"
     CDS             4746..6164
                     /gene="gltD"
                     /locus_tag="RPM73_003084"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDX6521419.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6348..7475
                     /locus_tag="RPM73_003085"
     CDS             6348..7475
                     /locus_tag="RPM73_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDX6521420.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEVLINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDTGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7535..7999)
                     /locus_tag="RPM73_003086"
     CDS             complement(7535..7999)
                     /locus_tag="RPM73_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDX6521421.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7996..8871)
                     /gene="nanK"
                     /locus_tag="RPM73_003087"
     CDS             complement(7996..8871)
                     /gene="nanK"
                     /locus_tag="RPM73_003087"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDX6521422.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8868..9557)
                     /locus_tag="RPM73_003088"
     CDS             complement(8868..9557)
                     /locus_tag="RPM73_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDX6521423.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9605..11095)
                     /gene="nanT"
                     /locus_tag="RPM73_003089"
     CDS             complement(9605..11095)
                     /gene="nanT"
                     /locus_tag="RPM73_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDX6521424.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11204..12097)
                     /gene="nanA"
                     /locus_tag="RPM73_003090"
     CDS             complement(11204..12097)
                     /gene="nanA"
                     /locus_tag="RPM73_003090"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDX6521425.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12219..13010)
                     /gene="nanR"
                     /locus_tag="RPM73_003091"
     CDS             complement(12219..13010)
                     /gene="nanR"
                     /locus_tag="RPM73_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDX6521426.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3081 a         3612 c         3519 g         2821 t
ORIGIN      
        1 ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc
       61 gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc
      121 tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta
      181 tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg
      241 ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg
      301 ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc
      361 gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta
      421 aaaccggcga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg
      481 cgcaggagcg cggctggcgt ttagcaaaaa actacgccgt cgggatgctc ttcctgaata
      541 aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg caacgcgaaa
      601 ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg
      661 ccctctcctc tctgccccac attgagcaaa ttttcgttaa cgccccggca ggctggcgtc
      721 cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag
      781 ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt gaacatctat aaaggtctgt
      841 gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg
      901 ccatctgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc
      961 agccgttccg ctacctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat
     1021 gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgatctg cacgacgccg
     1081 caccgttcgt caacgaaacc ggttctgact ccagttcgat ggataacatg ctggaactgc
     1141 tgctggcagg cgggatggat atcatccgcg ccatgcgcct gttagtacca cccgcctggc
     1201 agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata
     1261 tggagccatg ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta
     1321 acctcgaccg taacggtctg cgtccggcac gctacgtcat taccaaagac aagctcatca
     1381 cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc gaaaaaggcc
     1441 gcgtcgggcc aggcgaactg atggttatcg acacccgcag tgggcgtatt ctgcactcgg
     1501 cagaaaccga tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg
     1561 tccgccgtct ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg
     1621 acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact
     1681 ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgacgaca
     1741 ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt
     1801 ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg
     1861 ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt
     1921 taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag
     1981 aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg
     2041 aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt
     2101 tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg
     2161 cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca
     2221 tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggcttcggcg
     2281 cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta gacacccatg
     2341 cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct
     2401 tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac
     2461 tgtttgaagc ggtcggtctg cacgatgatg tagtgggcct gtgcttccag ggggcggtca
     2521 gccgcattgg tggggcgagc tttgaagact tccagcagga tctgctgaac ctgtcgaaac
     2581 gtgcctggct ggcgcgtaag cccatcagcc agggcggcct gctgaaatac gtccacggcg
     2641 gcgaatacca cgcttacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg
     2701 gcgagtacag cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc
     2761 tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac
     2821 cggcaagcga actgtttaaa cgctttgata ccgccgcgat gtctatcggc gcgttaagcc
     2881 cggaagccca cgaggcgctg gcggaagcga tgaatagcat cggcggtaac tcgaactccg
     2941 gtgaaggcgg cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg
     3001 tggcttctgg tcgcttcggc gttactccgg cgtatctggt caatgccgac gtcattcaga
     3061 ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca gttgccgggc gacaaagtca
     3121 ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc
     3181 cgcaccacga tatctactct atcgaggact tagcgcagct cattttcgac ctcaagcagg
     3241 ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta ggcaccatcg
     3301 cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat gacggtggca
     3361 ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag ctggggcttg
     3421 ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg
     3481 atggcggcct gaaaaccggt gttgatatca tcaaagcggc gattctcggc gcagaaagct
     3541 tcggcttcgg cactggcccg atggtggcgc tcggctgtaa atatctacgt atttgccatc
     3601 tgaacaactg cgcaacgggt gtagcaactc aggatgacaa actgcgtaag aaccactatc
     3661 acggcctgcc gttcaaggtt accaattact ttgaatttat tgcccgtgaa acccgcgagc
     3721 tgatggcaca gctgggcgta acgcgtctgg tggatctgat tggtcgcacc gacctgctga
     3781 aagaactgga cggtttcacc gccaaacagc aaaaactggc gctgtcgaag ctgctggaga
     3841 ccgccgaacc gcatccaggt aaggcactct actgcaccga aaacaaccca ccgtttgata
     3901 acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga
     3961 gcaaaacctt ctggttcgat attcgcaaca ccgaccgttc tgtcggcgcg tcgctttcag
     4021 gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact
     4081 tcaacggcac cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc
     4141 tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc
     4201 gtcctccggt tggttccgcc ttccgcagcc atgaagcaag tattatcggc aacacctgcc
     4261 tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg
     4321 tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata
     4381 tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggt gcgggcatga
     4441 ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg
     4501 aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc
     4561 ttatcaccga gcatgttcag catactggct ctcagcgcgg tgaagagatt ctggcgaact
     4621 ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac
     4681 tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag
     4741 caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga
     4801 aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc
     4861 aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat
     4921 gtccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg cgtatttttg
     4981 aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtttgcgga cgagtctgcc
     5041 cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga tgagtttggc gcggtgacca
     5101 tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata
     5161 tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggcgcaggc ccggcaggtc
     5221 tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc
     5281 cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa
     5341 tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag
     5401 tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtc ttccttggcg
     5461 tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg
     5521 cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg
     5581 acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg
     5641 cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa gcacgttacc tgtgcctatc
     5701 gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag
     5761 gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt aacggtaaag
     5821 tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc
     5881 gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcagtg atcatggcgt
     5941 ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac
     6001 aaggccgcat catcgccccg gaaggcagcg acaacgcctt ccagaccagc aacccgaaaa
     6061 tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtaaccgct attgccgaag
     6121 gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagctctg tcccagcgcc
     6181 gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaag cgcctcgcat
     6241 aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt
     6301 atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct
     6361 cagagagaac ctcaacaggc taccagcaaa tccacgacgg tattattcat ctggtcgata
     6421 gcgcccggac ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa
     6481 ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc cagggctgcg tatggtgcgc
     6541 agctaatcaa gcgactatca aaggatttaa gtctaaggta taagcgtggg ttctctgcaa
     6601 aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga
     6661 cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat
     6721 cggcaaagat ttggcagacg ctgtctgcca aatctttccc cctgccctgg tccacctacg
     6781 tccgtttact ttccgttaaa aacgctgacg cccgcagctt ttatgaaaaa gagacgctcc
     6841 gctgtggctg gtctgttcgc cagctagaac ggcaaattgc gacccaattt tatgagcgaa
     6901 cactactgtc acatgacaaa tcagccatgc tgcaacaaca cgctcctacc gagacgcata
     6961 ttcttccgca acaggcgata cgcgatccct ttgtgctcga atttctggaa ttgaaagatg
     7021 aatattcaga atctgatttc gaggaggtgc tgatcaacca cctgatggat ttcatgctgg
     7081 aacttgggga tgattttgcc tttgttggtc ggcagcgaag gttacgcatt gatgacaact
     7141 ggtttcgtgt cgatctgctg tttttccacc gccgtttacg ctgcctacta atcgtcgatc
     7201 taaaagtggg caaattcagc tatagcgata ccggacagat gaatatgtat ctcaactacg
     7261 ccaaagagca ctggacgcta ccggatgaaa atccgcccat cggtctggtt ctctgtgcag
     7321 agaaaggagc cggagaagca cattatgctc tggcaggttt gcctaacacc gttctggcaa
     7381 gcgaatataa gatgcaacta cctgatgaga aacgactcgc agatgaactc gttcgaacac
     7441 aggcggtgct agaggaaggc tatagacgcc gttaatttca gcggtttcga attctgcaga
     7501 cagtgtctgc agaatcttta aaacgaacta agttttaagc cattaaatca gccttaacct
     7561 tcaccacaac ctttttaatc tctccaggct cgccgacaac gcatcctggt ttatgcggtt
     7621 cacctggcat aaacacggcg aacattcccg gttttaagat gatggcttgc tcgttatcaa
     7681 tggtgctgca aagctgataa tcatcctcat ggtggaactc ttcacactgg cgcgcagtgc
     7741 ctgccatgcc aaacagaatc cgttcctcac cgtttaataa cagttggata tcaatgtatt
     7801 gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc atgacattca
     7861 taaagatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc ggtctggcag
     7921 ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgact
     7981 gtacttcacc catcatcata atttttctcc ctgggccaac agcgcagccc caagtaaacc
     8041 tgcatcgtgg cggtagtgcg ccgccagtaa atcaacatga aatgccgctg gctcctgcgc
     8101 cagatacgtt tccaccagcg ccagataccc ttctgccagc ccaacgctgc caccgaccac
     8161 cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcaa gcgtacgtgc
     8221 ggagcggtga atcagctgct gcgcctgctc gtcgccctgc ccggcgcgtg tgaaaatagt
     8281 tctcgcatcc gcgcccgcca gttcaccctg cgctgccgcc gcaatgccgc gaccagaagc
     8341 aatcgcctcc acgcaacctg tgcgtccaca gccacagacc gggccgtgtg gatcggcaag
     8401 cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttgc cgccgctcac
     8461 tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta catcgccttc
     8521 cagcgcctga tactccgccc atgctgcagc ctgcgcgtcg ttaatggcaa tggtcggcaa
     8581 atcggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac caagattatg
     8641 cggattcagc gccagcaagc tgccgtcgcg aataatcccg gttgaagcga tggcaacccg
     8701 ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca aggcttctgg
     8761 tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt cagcgccaat
     8821 cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agcgtggtca tagcaccgcc
     8881 tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg
     8941 accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa
     9001 cgcccttcgg caatcaccct acatccggcg tcgctcaacg ttttcaccag cgccagatcc
     9061 ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct
     9121 cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa
     9181 ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg
     9241 ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcaacatc ttcaatatag
     9301 gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg
     9361 ctcaccaccg cacgcgtggc ttgcagattt gccacacctt caatgcgaat ggcaaccgcg
     9421 cccgcctgtt ctgccgctaa tgccatggcg gcgacgattt cgggtttatc gagcgggctg
     9481 tccggaaccg gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt
     9541 tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag accgggcaat
     9601 aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc acggcaccgc
     9661 taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct
     9721 gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc agactgaacg
     9781 atagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg
     9841 gggccagtgc gccgcccaat gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct
     9901 ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa
     9961 gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg ccaatcgcaa
    10021 ataccggaat aatcaacagc tgcgaggcca acaggctaca aacgtaggct ttgcgggttc
    10081 ccagccagtc accgaggaag ccgccgacac agcaccccac cgcagcccca aagccgctaa
    10141 agaacagcac attggcgaca gtatgcgggt cataagccag atcggttttc agatacgttg
    10201 gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca
    10261 ttacgcccgt tggccagcgt ttgcccgtac tctgcaccat aaagctgata aagattgcgg
    10321 cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaga
    10381 accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac
    10441 ggtagagaat atccaccatt gtgcgtaccg gtgctttacc cccgtgtttc tctttccagt
    10501 cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc aaaatgccga
    10561 taaagaacag cgcacgccag ccccagaccg gaaccaccag gctatagacc tgagcggcaa
    10621 cgacggcccc cacagagaag cctgaaatca gaaaaccact ggctttgtta cgcagatgtt
    10681 ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc
    10741 cgatgaccag acgcgcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg
    10801 tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac
    10861 ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac
    10921 ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt accagggcga
    10981 ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc
    11041 attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga
    11101 gtctcccgcg gtgggcgatg cccacacgct ttggtataaa aattgtaggg tacagatgcg
    11161 tttatttccc ctcacccggt aggggcgagc gaggggaaac aactcacccg cgctcttgca
    11221 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
    11281 gtttgcggca cagcggcaca gaaacgacat ccatataatg gaggacagtt ttcaggccgc
    11341 ggaatacgcc cgttttgatc agtaaatcaa tgactttatt gcattcagtt tgcagtttct
    11401 gcgcggtctg gatatcgcct tctttcagcg ccttaacgat cccctgatag cgccagccca
    11461 tgatgttgta ggtactaccg ataccaccat cagcgcccgc cagcagacca gaggcgaaga
    11521 tttcgtcgta accgttatag agcacaagat caggatgttc acgacggatc tgctccatct
    11581 gatagagatc gccagaggtc tgtttcagcg cacctacgcc aggcaatgta acaagtgtgt
    11641 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
    11701 gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
    11761 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
    11821 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
    11881 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
    11941 caaaggcctc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
    12001 actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttggtca aaaggagtca
    12061 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
    12121 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
    12181 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
    12241 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
    12301 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
    12361 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
    12421 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
    12481 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
    12541 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcatgttc agccgcatag
    12601 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
    12661 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaagga
    12721 cgtgagacgc gggcacgttc gccgttgttg atttgcacca gacctttgcg ttttaacgct
    12781 gccagcgctt cgcgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
    12841 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
    12901 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
    12961 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
    13021 cagtgtgatt aac
//
LOCUS       DAPPNJ010000131        12987 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000131 DAPPNJ010000000
VERSION     DAPPNJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.132"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            44..379
                     /locus_tag="RPM73_003092"
     CDS             44..379
                     /locus_tag="RPM73_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDX6521427.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            539..982
                     /gene="yjdN"
                     /locus_tag="RPM73_003093"
     CDS             539..982
                     /gene="yjdN"
                     /locus_tag="RPM73_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDX6521428.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1115..1903
                     /gene="phnC"
                     /locus_tag="RPM73_003094"
     CDS             1115..1903
                     /gene="phnC"
                     /locus_tag="RPM73_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDX6521429.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1928..2944
                     /gene="phnD"
                     /locus_tag="RPM73_003095"
     CDS             1928..2944
                     /gene="phnD"
                     /locus_tag="RPM73_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6521430.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFANNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3005..3784
                     /gene="phnE"
                     /locus_tag="RPM73_003096"
     CDS             3005..3784
                     /gene="phnE"
                     /locus_tag="RPM73_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDX6521431.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3805..4530
                     /gene="phnF"
                     /locus_tag="RPM73_003097"
     CDS             3805..4530
                     /gene="phnF"
                     /locus_tag="RPM73_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDX6521432.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4531..4983
                     /gene="phnG"
                     /locus_tag="RPM73_003098"
     CDS             4531..4983
                     /gene="phnG"
                     /locus_tag="RPM73_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDX6521433.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            4980..5564
                     /gene="phnH"
                     /locus_tag="RPM73_003099"
     CDS             4980..5564
                     /gene="phnH"
                     /locus_tag="RPM73_003099"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDX6521434.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5564..6628
                     /gene="phnI"
                     /locus_tag="RPM73_003100"
     CDS             5564..6628
                     /gene="phnI"
                     /locus_tag="RPM73_003100"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDX6521435.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGERPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6621..7466
                     /gene="phnJ"
                     /locus_tag="RPM73_003101"
     CDS             6621..7466
                     /gene="phnJ"
                     /locus_tag="RPM73_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDX6521436.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7463..8221
                     /gene="phnK"
                     /locus_tag="RPM73_003102"
     CDS             7463..8221
                     /gene="phnK"
                     /locus_tag="RPM73_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDX6521437.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8332..9012
                     /gene="phnL"
                     /locus_tag="RPM73_003103"
     CDS             8332..9012
                     /gene="phnL"
                     /locus_tag="RPM73_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDX6521438.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9009..10145
                     /gene="phnM"
                     /locus_tag="RPM73_003104"
     CDS             9009..10145
                     /gene="phnM"
                     /locus_tag="RPM73_003104"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDX6521439.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10145..10702
                     /gene="phnN"
                     /locus_tag="RPM73_003105"
     CDS             10145..10702
                     /gene="phnN"
                     /locus_tag="RPM73_003105"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDX6521440.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10689..11123
                     /gene="phnO"
                     /locus_tag="RPM73_003106"
     CDS             10689..11123
                     /gene="phnO"
                     /locus_tag="RPM73_003106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDX6521441.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11125..11883
                     /gene="phnP"
                     /locus_tag="RPM73_003107"
     CDS             11125..11883
                     /gene="phnP"
                     /locus_tag="RPM73_003107"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDX6521442.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12030..12359)
                     /gene="yjdP"
                     /locus_tag="RPM73_003108"
     CDS             complement(12030..12359)
                     /gene="yjdP"
                     /locus_tag="RPM73_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDX6521443.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12428..12877)
                     /gene="rpiB"
                     /locus_tag="RPM73_003109"
     CDS             complement(12428..12877)
                     /gene="rpiB"
                     /locus_tag="RPM73_003109"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDX6521444.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
BASE COUNT         2845 a         3937 c         3644 g         2561 t
ORIGIN      
        1 gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat taccacactg
       61 cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct gcccggaatg
      121 tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga tcgttaaaga
      181 tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag atctgaaggt
      241 gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc gcctggttga
      301 aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac tgaaatctga
      361 gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc
      421 atccggcaac gatgccgact gcctgatgcg acgctgaccg cgtcttatca ggcttccctc
      481 tcatctgtat aaatttcgaa ctacacttaa ctggcttctc ttaactgagg tcaccatcat
      541 gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg cttattatca
      601 acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc caaaatcagc
      661 gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg ccatcgctca
      721 tgccaacgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc cgtcaggaaa
      781 agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag aaggaaaacg
      841 ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg gcctggcagg aaactttctg
      901 ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga ttaatgtcgt
      961 caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc ctcccgcact gtcatcgaat
     1021 tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac aataatccgc
     1081 cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc gtgtcgagaa
     1141 gctcgccaaa accttcaatc agcatcaggc gctgcatgcg gttgatctga acattcatca
     1201 cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc ttttacgtca
     1261 cttaagcggt ttgattaccg gcgataaatc cgccggcagc catatcgagc tgctgggccg
     1321 cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg ccaacaccgg
     1381 ctacatcttc caacaattca acctggtgaa ccgcctgagc gtactggaga acgtgctgat
     1441 tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttca ccggcgagca
     1501 gaaacagcgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg cccatcagcg
     1561 cgtttccacc ctctccggcg gacagcagca gcgtgtggcg attgcccgcg cgctgatgca
     1621 gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg aatccgcccg
     1681 catcgtgatg gacaccctgc gcgacatcaa ccagaacgac ggcatcaccg tggtcgtcac
     1741 gctacatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc tgcgccaggg
     1801 gcacgttttc tacgacggca gcagccaaca gtttgataac gaacgttttg accatctcta
     1861 ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc atcattgagg
     1921 aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt tcagcctcag
     1981 caccctgtta agcccggcac acgccgaaga gcaggaaaag gcgctgaatt tcggcattat
     2041 ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg ccattcttac aggatatgga
     2101 gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca gactacgcag gcattatcca
     2161 gggaatgcgc ttcaataaag tggatatcgc ctggtacggc aacctgtcgg caatggaagc
     2221 ggtggatcgc gccaacggcc aggtctttgc ccagacggtc gcggcggatg gatcgccggg
     2281 ttactggagc gtgttgatcg tcaacaaaga cagcccgatc aacaacctga acgatctgct
     2341 ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct aactccacct ctggcttcct
     2401 cgtccccggt tactacgtct ttgccaataa caatatctcc gccagcgact ttaagcgcac
     2461 cgtcaacgct gggcatgaaa ctaacgcgct ggccgtcgcc aacaagcagg tggatgttgc
     2521 caccaacaac accgaaaacc tcgacaagct gaaaacctcc gcgccagaga agctgaaaga
     2581 actgaaggtg atctggaagt cgccgctgat cccaggcgat ccgatcgtct ggcgcaagaa
     2641 tctctccgaa accaccaaag acaagatcta cgacttcttt atgaactacg gaaaaacgcc
     2701 ggaagagaaa gcggtgctgg aacgcctggg ctgggccccg ttccgcgcct ccagcgacct
     2761 gcaactggtg ccgattcgcc agctcgcact gtttaaagag atgcagggcg taaaaagcaa
     2821 taaaggactg aatgagcagg acaagctggc gaaaaccacc gagattcagg cgcagctgga
     2881 tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta aagcggtgca
     2941 gtaatgtgct cgatcggtcc cctctcccac agggagaggg agaaaacatt aaacggagcc
     3001 aaacatgcaa accatcacca tcgccccacc caagcgtagc tggttctcgc ttctgagctg
     3061 ggccgttgtt ctcgccgtgc tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac
     3121 gctgattaaa gacggcggca acatggcaac cttcgctgcc gacttcttcc cgcccgattt
     3181 cagccagtgg caggattacc tcaccgaaat ggccgtcacg ctgcaaatcg ccgtctgggg
     3241 caccgcgctg gcggtggttc tctccatccc ctttggcctg atgagcgccg aaaacctggt
     3301 gccgtggtgg gtttaccagc ccgttcgccg cctgatggac gcctgccgcg ccattaacga
     3361 aatggtcttc gccatgctgt tcgtggtcgc cgtcggtctc ggaccgttcg ctggcgtgct
     3421 ggcgctattt atccacacca ccggcgtgct ctccaagctg ctttccgagg cggtggaagc
     3481 gattgaaccc ggcccggtgg aaggcattcg cgccaccggt gccaacaagc tcgaagagat
     3541 cctctacggc gtgctgccac aggtgatgcc gctgctgatc tcctactccc tctatcgctt
     3601 cgaatccaac gtccgctcgg cgaccgtcgt cggcatggtc ggcgcaggcg ggatcggcgt
     3661 caccctgtgg gaagcgattc gcggtttcca gttccagcaa acctgcgccc tgatggtgct
     3721 tatcatcgtc acggtcagcc tgctggattt cctctctcaa cggttgcgta agcactttat
     3781 ctgataagcg aggcattgat atctatgcac ttgtctacac atccgaccag ctacccaaca
     3841 cgctatcagg agatagccgc aaaacttgag caggagcttc gtcaacacta tcgctgcggc
     3901 gactatcttc ccgccgagca gcagctggcg gcgcgctttg aggtaaatcg ccacaccctg
     3961 cgccgcgcca tcgaccaact ggtggaaaaa ggctgggtac agcgccgtca gggcgtcggc
     4021 gtgctggtgc tgatgcgccc gttcgattac ccgctcaacg cccaggcgcg ttttagccag
     4081 aacctgctgg atcagggcag ccatcccacc agcgaaaaac tgctgtcggt gctgcgcccg
     4141 gcatctggtc acgtcgctga cgcgctgggg ataaccgagg gggagaacgt catccacctg
     4201 cgcaccctgc gccgggtcaa cggcatcgcg ctctgtttaa tcgaccacta ctttgcggac
     4261 ctcaccctct ggccgacact gcaacgtttc gacagcggtt cgctgcacga ttttctgcgc
     4321 gagcaaaccg gaattgcgct gcgccgcagc cagacgcgga tcagcgcccg ccgcgcccag
     4381 gccaaagagt gccagcgcct tgaaatcccg aatatgtcgc cgctgctgtg cgtgcgcacc
     4441 cttaaccacc gtgacggtga aagcagcccg gcggagtact ccgtcagcct gacgcgcgcc
     4501 gacatgattg aattcactat ggagcactga atgcacgcag ataccgcgac ccgccagcac
     4561 tggatgtccg tactggcgca cagccaaccg gctgaactgg cagcccgcct gaacgcgctg
     4621 aacatcaccg ccgactatga agtgatccgc actgccgaaa ctggcctggt gcagattcag
     4681 gcgcgaatgg gcggtaccgg cgaacgcttt tttgccggcg acgccacgct gacccgcgcc
     4741 gccgtacgcc tgactgacgg cacgctcggc tacagctggg tgctggggcg cgacaaacag
     4801 cacgccgaac gctgcgcgct gattgacgcg ctgatgcagc aatctcgcca ctttcaaaac
     4861 ttatcagaaa ctcttattgc cccgctggac gctgaccgta tggcacgcat tgccgcacgc
     4921 caggccgaag tgaacgccag ccgggtcgac ttctttacga tggttcgcgg agataacgca
     4981 tgaccctgga aaccgctttt attcttcctg tgcaggatgc ccagcacagt tttcgtcgcc
     5041 tgttaaaggc catgagcgag ccgggcgtga ttgtcgccct gcatcagctc aaacgcggct
     5101 ggcagccgct gaatatcgcc accaccagcg tgctgctgac gctggccgat aacgacacgc
     5161 cggtgtggct ttccgcgcca ttgagtaacg acatcgtcag ccagagcctg cgttttcata
     5221 ccaacgcgcc gctggtcagc cagccggaac aggcgacctt cgcggtaacg gatgaggcga
     5281 tttccagcga acagcttaac gccctgtcca ccggcaccgc cgttgcgccg gaagcgggtg
     5341 caacgctgat tttacaggtc gccagcctga gcggcggacg catgttacgt ctcaccggcg
     5401 cgggcattgc cgaagaacga atgatcgccc cgcagctgcc ggagtgcatt ctgtacgaac
     5461 tcaccgagcg cccgcacccg ttcccgctcg gcatcgacct gatcctgacc tgcggcgagc
     5521 gcctgctggc tattccgcga accacgcatg tggaggtgtg ctgatgtacg ttgccgtgaa
     5581 agggggcgaa aaggcgatcg atgccgccca cgccctgcaa gagagccgac gccggggcga
     5641 taccgatttg cctgaactga gcgtcgccca gattgaacag cagcttaacc tcgcagtaga
     5701 tcgcgtgatg accgaaggcg gcattgccga ccgcgaactg gcggcgctgg cgctgaaaca
     5761 ggccagcggc gataacgtcg aagcaatttt cctgctgcgc gcctaccgca ccacgctggc
     5821 gaagctcgcg gtaagcgagc cgctcgacac cacggagatg cgtctcgaac ggcgtatttc
     5881 agcggtttat aaagatattc ccggcggcca gttgcttggc ccaacctacg actacaccca
     5941 tcgcctgctc gatttcaccc tgctggcaaa cggcgaagcg ccgacgctga ccaccgccca
     6001 cagcgaacaa cagccgtcgc cgcacgtttt cagcctgctg gcgcgtcagg ggctggcgaa
     6061 gtttgaagag gatagcggcg cacagccgga tgacatcacc cgcacgccgc cggtttaccc
     6121 ctgctcacgc tcctcccgtt tacagcagtt gatgcgcggc gacgaaggct atttgctggc
     6181 gctggcctac tccacccaac gcggctacgg tcgcaaccac ccgttcgcag gcgagatccg
     6241 cagcggctat atcgacgtat cgattgtgcc ggaagagctg ggatttgcgg tgaacgtcgg
     6301 cgaactgctg atgaccgagt gtgaaatggt caacggtttt atcgacccgc cgggtgagcg
     6361 gccgcacttc acgcgcggct acgggctggt gttcggcatg agcgagcgca aagcgatggc
     6421 aatggcgctg gtcgatcgtg cgttgcaggc tccagagtac ggcgagcacg cgacaggccc
     6481 ggcgcaggat gaagagttcg tgctggcgca tgccgacaat gtcgaagccg caggctttgt
     6541 ctcgcacctg aaactcccgc actacgtcga tttccaggcc gaactggagc tactcaaacg
     6601 tctgcaacag gagcagaacc atggctaatc tgagcggcta caactttgcg tacctcgacg
     6661 agcagaccaa acgcatgatc cgccgcgcca tcttaaaagc ggtggcgatc cccggctatc
     6721 aggtgccgtt tggcgggcgc gagatgccga tgccatacgg ctggggaacc ggcggcatac
     6781 agctcaccgc cagcgtgatt ggcgaaagcg acgtgctgaa ggtgattgac cagggcgcag
     6841 acgacaccac caacgccgtg tcgattcgca acttctttaa gcgcgtgacc ggggtaaaca
     6901 ccactgaacg tacggacgat gcgacgctta tccagacgcg tcaccgcatc cccgaaacgc
     6961 cgctgaccga agatcagatc attatcttcc aggtgccaat cccggaaccg ctgcgcttta
     7021 tcgagccgcg cgaaacggag acccgcacta tgcacgcgct ggaggagtac ggcgtgatgc
     7081 aggtgaaact gtatgaagat atcgcccgct tcggtcatat cgccaccacc tatgcttatc
     7141 cggtgaaggt gaacgggcgc tacgtgatgg acccgtcgcc gatcccgaaa ttcgataacc
     7201 cgaaaatgga catgatgccc gccctgcagc tgttcggagc agggcgcgag aagcgcatct
     7261 atgcggtgcc gccgtttacc cgcgtggaaa gtctcgattt cgacgatcac ccgttcaccg
     7321 ttcagcagtg ggatgagcca tgcgccatct gcggatcgac ccacagctat cttgatgaag
     7381 tggtgctgga tgacgccgga aaccgcatgt ttgtctgctc cgataccgat tattgccgcc
     7441 aacagagcga ggcgaaaagc caatgaataa accgttactt tcggtcaata acctgaccca
     7501 cctttacgcg ccgggcaaag gctttagcga tgtctctttt gatttatggc cgggagaagt
     7561 gctgggcatt gtcggagaat ccggctcagg gaagaccacg ctgctgaaat caatctccgc
     7621 gcgcctgacg ccgcagcagg gggagattca ctatgagagc cgttcgctgt acgcaatgag
     7681 cgaggccgac cgccgtcgcc tgctgcgcac cgaatggggc gtagtgcatc agcatccact
     7741 cgacggcctg cgccgtcagg tctcggcagg cggcaatatc ggcgagcggc tgatggcgac
     7801 cggggcacgt cattacggcg atattcgtgc caccgcgcag aagtggctgg aagaggtgga
     7861 aattcccgcc aaccggatcg acgacctgcc gaccaccttt tccggcggca tgcagcaacg
     7921 tttgcagatc gcccgcaacc tggtgacgca tccgaagctg gtgtttatgg acgagccgac
     7981 cggcggactg gatgtgtcgg tacaggcacg cctgctcgac ctgctgcgcg gcctggtggt
     8041 ggagctgaac ctcgcggtag tgattgtcac ccacgattta ggcgtcgccc gcctgctggc
     8101 ggaccgtttg ctggtgatga agcaggggca agtggtggag agtgggttaa ccgaccgcgt
     8161 gctcgacgac ccgcaccatc cgtatacaca actgctggtg tcatcggttt tgcagaattg
     8221 agccggtgcc ggatgcggcg taaacgcctt atccggccta caaatgcgct ccccgtaggt
     8281 cggataagac gcgtcagcgt cgcatccgac acccgaacca cgaggcgaaa aatgattaac
     8341 gtacaaaacg tcagtaaaac cttcatcctg caccagcaaa acggcgtgcg cctgcccgtc
     8401 ttacaacgtg cctcgctcac cgtcaacgcg ggcgaatgcg tggtgctcca cggccattcc
     8461 ggcagcggca aatcaacctt gctgcgctcg ctgtacgcca actatctgcc cgacgaaggg
     8521 caaatccaga tcaaacacgg tgacgaatgg gtcgatctgg tcaccgcgcc agcgcgcaaa
     8581 gtggtggaga tccgcaaaac caccgtcggc tgggtgagcc agtttctgcg cgtcatcccg
     8641 cgtatttcag cactggaagt ggtgatgcag ccgctgctcg ataccggcgt tccgcgtgaa
     8701 gcctgcgccg ctaaagccgc gcgtctgctc acccgcctga acgtgccgga acgcctgtgg
     8761 cacctggcgc catcaacctt ttccggtggc gaacagcagc gcgtcaacat cgcccgcggc
     8821 tttatcgtcg actaccccat tctgctgctc gacgaaccta ccgcctcgct ggacgccaaa
     8881 aacagcgccg ccgtagtcga gctgattcgc gaagccaaaa cccgtggcgc ggccatcgtc
     8941 ggcatcttcc atgacgaagc cgtacgtaat gacgtcgccg accgcttgca cccaatggga
     9001 gcctctgcat gattatcaat aacgtcaaac tggtgctgga aaacgaagtg gtacacggtt
     9061 cgctggaggt gcaggatggc gaaatccgcg cctttgccga aagccagagc cgcctgccag
     9121 aagcgatgga cggcgaaggt ggctggctac tacccggcct gattgagctg cataccgaca
     9181 acctcgataa attcttcacc ccgcgcccga aagtcgactg gcccgcccat tcggcgatga
     9241 gcagccacga cgcgctgatg gtggcaagcg gcatcaccac cgtactggac gccgtggcga
     9301 ttggtgatgt gcgcgacggc ggcgatcggc tggagaatct ggagaagatg atcaacgcca
     9361 tcgaagagac gcaaaaacgc ggcgtcaacc gcgccgagca ccgcctgcac ctgcgctgcg
     9421 aactgccgca tcacaccaca ctgccgctgt ttgaaaaact ggttcagcgc gaaccggtga
     9481 cgctggtgtc gctgatggac cactcaccgg gccagcgcca gttcgctaac cgcgagaagt
     9541 atcgcgaata ttatcagggc aaatactccc tcaccgatgc gcagatgcag cagtacgaag
     9601 aagagcaatt ggcgctcgcc gcacgctggt cgcagccgaa tcgcgaatcc atcgccgccc
     9661 tgtgccgcgc gcgacaaatt gcgctcgcca gccacgatga cgccacgcac gcccacgttg
     9721 ccgaatccca ccaacttggc agcgtgatcg ccgaatttcc caccacgttc gaagcggcgg
     9781 aagcctcgcg taagcatggc atgaacgtgc tgatgggtgc gccaaatatc gtgcgtggcg
     9841 gctcgcactc cggcaatgtg gcggccagtg aactggcgca gcttggtctg ctggatatcc
     9901 tctcttccga ctactacccc gccagcctgc tggatgcggc gttccgcgtc gccgatgacg
     9961 agagcaaccg ctttacgcta ccgcaggcgg tgaggctggt gaccaaaaat ccggcgcagg
    10021 cgctgaatct tcaggatcgc ggggtgattg gcgagggtaa acgcgctgac ctggtgctgg
    10081 cgcatcgcca gggcaatcac attcatatcg accacgtctg gcgtcagggt aaaagggtgt
    10141 tctgatgatg ggaaaactga tttggttaat ggggccgtcc ggctccggga aagacagcct
    10201 gctggcggaa ctccgcctgc gggaacaaac gcagttactg gtggcgcatc gctacatcac
    10261 gcgcgacgcc agcgccggaa gtgaaaacca tatcgccctg agtgagcggg agttttttac
    10321 ccgcgcgggg caaaacctgc tggccttaag ctggcacgct aacggtctgt attatggcgt
    10381 cggcgtcgag attgacctct ggctgcacgc tggattcgac gtgctggtca acggctcacg
    10441 cgcccatctg ccgcaggcgc gggcgcgcta tcaatcggcg ctgctgccca tttgcttaca
    10501 ggtttcgcca gagatcctgc gccagcgcct ggaaaaccgt ggccgcgaaa acgccagtga
    10561 aattaacgcc cggctggcgc gcgccgcccg ctatactcca caggattgcc atacgctcaa
    10621 taatgacggc agcctgcgcc agtcggtcga cacgctgctg acgctaatcc atcagaagga
    10681 gaaacaccat gcctgcttgt gagcttcgcc cggccacgca gtacgacacc gacgcggttt
    10741 acgcgttgat ttgcgagcta aaacaggcgg agtttgacca ccacgcgttt cgcgtgggct
    10801 ttaacgccaa cctgcgcgac ccgaacatgc gctaccatct ggcgctgctg gatggcgaag
    10861 ttgtcggtat gatcggcctg catttgcagt ttcatctgca tcatgtcaac tggatcggcg
    10921 aaattcagga gttggtggta atgccgcagg cgcgcggtct gaacgtcggc agtaagttac
    10981 tggcgtgggc agaagaagaa gcccgccagg ccggggccga aatgaccgaa ctttcgacca
    11041 acgtgaagcg ccacgacgcg caccgtttct atctgcgaga aggctacgag cagagtcact
    11101 tccgcttcac caaggcgctg taacatgagt ctgaccctca cgctcaccgg caccggcggc
    11161 gcacagggcg ttccggcatg gggctgcgag tgcgcggcct gcgaccgagc gcggcgctca
    11221 ccgcagtatc gccgccagcc atgcagcggc gtagtgaagt ttaacgacgc aatcaccctg
    11281 atcgacgccg ggctgcacga tctcgccgat cgctggtcgc ccggatcgtt ccagcagttt
    11341 ttgctgacgc attatcatat ggatcacgtc caggggctgt ttccgctgcg ctggggcgtt
    11401 ggcgatccga tcccggttta cggcccgccg gatgaccagg gttgcgacga tctgtttaaa
    11461 catccgggcc tgcttgattt cagccacaca gtggaaccgt ttgtggtatt tgatttgcag
    11521 gggttgcagg tcacgccgct gccgcttaac cactcaaaac tgaccttcgg ttatctgctg
    11581 gaaacggcac atagccgggt ggcgtggcta tctgacaccg ctggcctgcc ggaaaaaaca
    11641 cagaaatttt tactcaataa tcatccgcag gtgatggtga ttgattgcag ccacccgccg
    11701 cgcgaggatg cgccgcgtaa tcactgtgat ttaaataccg tgcttgcgct caatcaggtt
    11761 atccactcgc cacgggtgat tctgacccat atcagccacc agtttgatgc gtggctgatg
    11821 gagaatgcgc tgccgccggg ttttgaggcg gggtttgatg ggatggagat tggggtggcg
    11881 tgatgagagg gaatgtgcgc gctggccccc tcaccctaac cctctcccca gaggggcgag
    11941 gggaccgatt gtgctcgata ttgaatattg cgctggtttt ctccctctcc ccattggggt
    12001 gaggggcgat gcctgctcca tacccaacct catcgcccat actcatcttc cattctccgc
    12061 tcttcatctt ctagctgccg acgctcctga tcaagctggc gctggcgatc gtccagctgc
    12121 ctgcggcgat cttcaaactg gcggcggcgg tcgtcatatt gtctgcgccg atcgtcgctc
    12181 acttcacgct gccagccgtc gtcgcgcgaa tcttcatagt ctcgcccacg gtcagggtta
    12241 taagcgtcgt taatcgcctg ctgaatattg ccaatggtgt cgtcgataat atcggcctgg
    12301 gccggaacgg tggacagcgt gagcagggtg aataaaagaa atagcggaaa gcgtttcatt
    12361 agccaacctc aaaaagaaac tctatccaca ttagtcatta ctcatccatg caagtagtgg
    12421 atgaatctca atttctccac tgctctattg ccgtaatcgc ctccacgcgt tgttgatgac
    12481 gaccgccttc gtactgtgcg cccagccacg catccacaat catttttgcc agttcgaggc
    12541 caaccactcg tgaaccaaaa gccagcacgt tggtgtcgtt atgctgccgc gaaagttgcg
    12601 cggaataagg ttcgctacag acgaccgcgc gaattccggc aaacttgttc gccgctatcg
    12661 aaataccgac gccagtacca caaatcaaaa tcccgccatc aacctctccg ccagcaacag
    12721 ccagtgcgac ttgactggcg taatgtggat aatcagtacg ctctgacgac caggttcctt
    12781 tatcaatcac ttcaacgcca cgctcaacta aatgtgccac tatttcatgt tttaaaatga
    12841 aaccgacatg atcacagcca aatgcaatct ttttcattca tcttccccgt ccgtgcaaaa
    12901 cttcacaatc aaaaaacatt tgtgatgtta atgaattaaa aacacccaaa atcgatgaat
    12961 tacgccttcc tgatctgttt tacagag
//
LOCUS       DAPPNJ010000132        12863 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000132 DAPPNJ010000000
VERSION     DAPPNJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.133"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            102..1211
                     /gene="livK"
                     /locus_tag="RPM73_003110"
     CDS             102..1211
                     /gene="livK"
                     /locus_tag="RPM73_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDX6521445.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1259..2185
                     /gene="livH"
                     /locus_tag="RPM73_003111"
     CDS             1259..2185
                     /gene="livH"
                     /locus_tag="RPM73_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDX6521446.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2182..3459
                     /gene="livM"
                     /locus_tag="RPM73_003112"
     CDS             2182..3459
                     /gene="livM"
                     /locus_tag="RPM73_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDX6521447.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3456..4223
                     /gene="livG"
                     /locus_tag="RPM73_003113"
     CDS             3456..4223
                     /gene="livG"
                     /locus_tag="RPM73_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDX6521448.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4225..4938
                     /gene="livF"
                     /locus_tag="RPM73_003114"
     CDS             4225..4938
                     /gene="livF"
                     /locus_tag="RPM73_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDX6521449.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5334..6650
                     /gene="ugpB"
                     /locus_tag="RPM73_003115"
     CDS             5334..6650
                     /gene="ugpB"
                     /locus_tag="RPM73_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDX6521450.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6748..7635
                     /gene="ugpA"
                     /locus_tag="RPM73_003116"
     CDS             6748..7635
                     /gene="ugpA"
                     /locus_tag="RPM73_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDX6521451.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7632..8477
                     /gene="ugpE"
                     /locus_tag="RPM73_003117"
     CDS             7632..8477
                     /gene="ugpE"
                     /locus_tag="RPM73_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDX6521452.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8479..9549
                     /gene="ugpC"
                     /locus_tag="RPM73_003118"
     CDS             8479..9549
                     /gene="ugpC"
                     /locus_tag="RPM73_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDX6521453.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9546..10289
                     /gene="ugpQ"
                     /locus_tag="RPM73_003119"
     CDS             9546..10289
                     /gene="ugpQ"
                     /locus_tag="RPM73_003119"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDX6521454.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWADMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10276..10716)
                     /locus_tag="RPM73_003120"
     CDS             complement(10276..10716)
                     /locus_tag="RPM73_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDX6521455.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10836..12578
                     /gene="ggt"
                     /locus_tag="RPM73_003121"
     CDS             10836..12578
                     /gene="ggt"
                     /locus_tag="RPM73_003121"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDX6521456.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAIDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     TKDGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12616..>12863)
                     /locus_tag="RPM73_003122"
     CDS             complement(12616..>12863)
                     /locus_tag="RPM73_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDX6521457.1"
                     /translation="YLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAARLAGNA
                     PFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2834 a         3306 c         3714 g         3009 t
ORIGIN      
        1 aatcagacag gcaaaaacag tgcagtataa aaaaagaaca gtctgatttg ttaacacata
       61 aaaacaaagc aacacaacat cacgaatggg gatttttgac tatgaaacgg aatgcgaaaa
      121 ctatcatcgc agggatgatt gcactggcaa tttcacacac cgctatggct gacgatatta
      181 aagtcgccgt tgtcggcgcg atgtccggcc cgattgccca gtggggcgat atggaattta
      241 acggcgcgcg tcaggcgatt aaagacatta atgccaaagg gggaattaaa ggcgataagc
      301 tggtgggcgt ggaatatgac gacgcctgcg acccgaaaca agccgttgcg gtcgccaaca
      361 aaatcgttaa cgacggcatt aaatacgtta ttggtcatct gtgttcttct tctacccaac
      421 ctgcatcaga tatctacgaa gacgaaggta ttctgatgat ctcgccggga gcgaccaacc
      481 cggagctgac ccaacgcggt tatcaacaca ttatgcgtac tgccgggctg gactcttccc
      541 aggggccaac ggcggcaaaa tacattcttg agacggtgaa gccccagcgc atcgccatca
      601 ttcacgacaa acaacagtat ggcgaagggc tggcgcgttc ggtgcaggac gggctgaaag
      661 cggctaacgc caacgtcgtc ttcttcgacg gtattaccgc cggggagaaa gatttctccg
      721 cgctgatcgc ccgcctgaaa aaagaaaaca tcgacttcgt ttactacggc ggttactacc
      781 cggaaatggg gcagatgctg cgccaggccc gttccgttgg cctgaaaact cagtttatgg
      841 ggccggaagg tgtgggtaac gcatcattgt cgaatattgc cggtgatgct gccgaaggca
      901 tgttggtcac tatgccaaaa cgctatgacc aggatccggc aaaccagggc atcgttgatg
      961 cgctgaaagc agacaagaaa gatccgtccg ggccttatgt ctggatcacc tacgcggcgg
     1021 tgcaatctct ggcgactgcc cttgagcgta ctggcagcga tgagccgctg gcgctggtga
     1081 aagatttaaa agctaacggt gcaaacaccg tgattgggcc gctgaactgg gatgaaaaag
     1141 gcgatcttaa gggatttgat tttggtgtct tccagtggca cgccgacggt tcatccacgg
     1201 cagccaagtg atcatcccac cgcccgtaaa atgcgggcgg gtttagaaag gttaccttat
     1261 gtctgagcag tttttgtatt tcttgcagca gatgtttaac ggcgtcacgc tgggcagtac
     1321 ctacgcgctg atagccatcg gctacaccat ggtttacggc attatcggca tgatcaactt
     1381 cgcccacggc gaggtttata tgattggcag ctacgtctca tttatgatca tcgccgcgct
     1441 gatgatgatg ggcattgata ccggctggct gctggtggcg gcgggattcg tcggcgcaat
     1501 cgtcattgcc agcgcctacg gctggagtat cgaacgggtg gcttaccgcc cggtgcgtaa
     1561 ttctaagcgc ctgattgcgc ttatttccgc aatcggtatg tccatcttcc tgcaaaacta
     1621 cgtcagcctg accgaaggtt cgcgcgatgt ggcgctgccc agcctgttta acggtcagtg
     1681 ggtggtgggc catagcgaaa acttctctgc ctctattacc accatgcagg cggtgatctg
     1741 gattgtcacc ttcctcgcca tgctggcgct gacgattttc attcgctatt cccgtatggg
     1801 gcgcgcctgt cgtgcctgcg cggaagatct gaaaatggcg agtctgcttg gcattaacac
     1861 cgaccgggtg attgcgctga cctttgtgat tggcgcggcg atggcggcgg tggcgggtgt
     1921 gctgctcggt cagttctacg gcgtaattaa cccctacatc ggctttatgg ccgggatgaa
     1981 agcctttacc gctgcggtgc tcggtgggat tggcagcatt ccaggggcga tgattggtgg
     2041 gctgatcctg gggattgcgg aggcgctctc ttctgcctat ctgagtacgg aatataagga
     2101 tgtggtttca tttgccctgc tgattctggt gctgctggtg atgccgaccg gtattctggg
     2161 tcgcccggag gtagagaaag tatgaaaccg atgcatattg caatggcgct gctctctgcc
     2221 gcgatgtttt ttgtcctggc gggcgtcttt atgggcgtgc aactggagct ggatggcacc
     2281 aaactggtgg tcgacagcgc ttcggatgtc cgttggcagt gggtgtttat cggcacggcg
     2341 gtggtctttt tcttccagct tttgcgaccg gctttccaga aagggctaaa aagcgtttcc
     2401 ggaccgaagt ttattctgcc cgcgattgat ggctccacgg tgaagcagaa actattcctc
     2461 gtggcgctgc tggtgcttgc ggtggcgtgg ccgtttatgg tgtcgcgtgg gacggtggat
     2521 atcgccaccc tgaccatgat ctacattatc ctcggcctcg ggctgaacgt ggttgttggt
     2581 ctttctggtc tgctggtgct ggggtacggc ggcttttacg ccatcggcgc ttacactttt
     2641 gcgctgctca atcactatta cggcctgggc ttctggacct gtctgccgat tgccgggtta
     2701 atggcagcgg cggccggctt cctgctcggc ttcccggtgc ttcgcttgcg cggcgactat
     2761 ctggcgatcg ttaccctcgg tttcggcgaa attgtgcgca tattgctgct caataacacc
     2821 gaaattaccg gcggcccgaa cggaatcagt cagatcccga aaccgacact cttcggactc
     2881 gagttcagcc gtaccgctcg tgaaggcggc tgggacacgt tcagtaattt ctttggcctg
     2941 aaatacgatc cctccgatcg tgtcatcttc ctctacctgg tggcgttgct gctggtggtg
     3001 ctaagcctgt ttgtcattaa ccgcctgctg cggatgccgc tggggcgtgc gtgggaagcg
     3061 ttgcgtgaag acgagatcgc ctgccgttcg ctgggcttaa gcccgcgtcg tatcaagctg
     3121 actgccttta ccataagtgc cgcgtttgcc ggttttgccg gaacgctgtt tgctgcgcgc
     3181 cagggctttg tcagcccgga atccttcacc tttgctgaat cggcgtttgt actggcgata
     3241 gtggtgctcg gcggtatggg atcgcagttt gcggtgattc tggcggcaat tttgctggtg
     3301 gtatcgcgcg aattgatgcg tgatttcaac gaatacagca tgttaatgct cggtggtttg
     3361 atggtgctga tgatgatctg gcgtccgcag ggcttgctgc ccatgacgcg cccgcaactg
     3421 aagctgaaaa acggcgcagc gaaaggagag caggcatgag tcagccatta ttatctgtta
     3481 acggtctgat gatgcgcttc ggcggcttgc tggcggtgaa caacgtcaat cttgaactgt
     3541 acccgcagga gatcgtctcg ttaatcggcc ctaacggtgc cggaaaaacc acggttttta
     3601 actgcctgac cggattctac aaacccaccg gcggcactat tttactgcgt gatcagcatc
     3661 tggaaggttt accggggcag caaattgccc gcatgggcgt ggtgcgtacc ttccagcatg
     3721 tgcgtctgtt ccgtgaaatg acggtgattg aaaacctgct ggtggcgcag catcaacaac
     3781 tgaaaaccgg gctgttctct ggcctgttga aaacgccttc tttccgtcgc gcccagagcg
     3841 aagcgctcga ccgcgccgcc acctggcttg agcgcattgg tttgctggaa cacgccaacc
     3901 gtcaggcgag taacctggcc tatggtgacc aacgccgtct ggagattgcc cgctgcatgg
     3961 tgacgcagcc ggagatttta atgctcgacg aaccggcggc aggtcttaac ccaaaagaaa
     4021 ccaaagagct ggatgagctg attgccgaac tgcgcaatca tcacaacacc actatcttgt
     4081 tgattgaaca cgatatgaag ctggtgatgg ggatttcaga ccgaatttat gtggtcaatc
     4141 aggggacgcc actggcaaac ggtacgccgg agcagatccg taataacccg gacgtgatcc
     4201 gtgcctattt aggtgaggca taagatggaa aaagtcatgt tgtcctttga caaagtcagc
     4261 gcccactacg gcaaaatcca ggcgctgcat gaggtgagcc tgcatatcaa tcagggcgaa
     4321 attgtcaccc ttattggcgc gaatggcgcg gggaaaacca ccttgctcgg cacattatgc
     4381 ggcgacccgc gcgccaccag cgggcgaatt gtgtttgatg ataaagacat taccgactgg
     4441 cagacggcga aaatcatgcg cgaagcggta gcgattgtcc cggaagggcg tcgcgtcttc
     4501 tcgcggatga cggtggaaga gaacctggcg atgggcggct tctttgccga acgtgaccag
     4561 tttcaggagc gcataaagtg ggtgtatgag ctgtttccac gtcttcatga gcgtcgtgtt
     4621 cagcgggcgg gcaccatgtc cggcggtgaa cagcagatgc tggcgattgg tcgcgcgctg
     4681 atgagcaacc cgcgtttgtt gttgcttgat gagccatcgc tcggtcttgc gccgattatc
     4741 atccagcaaa ttttcgacac catcgagcag ctgcgcgagc aggggatgac tatcttcctc
     4801 gtcgagcaga acgccaacca ggcgctaaag ctggcggatc gcggctacgt gctggaaaac
     4861 ggccatgtgg tgctctccga taccggcgac gcgctgctgg cgaacgaagc agtgagaagt
     4921 gcgtatttag gcgggtaata acacgttgat tgataaggag tcaaaagact cctttgagac
     4981 aggtgacaaa tgtaaaattg cctgatgcgc tgcgcttatc aggcctactg ggtgagttgc
     5041 aatatattga atttgcacga tcttgtaggc cggataaggc gtttacgccg catctggcat
     5101 gaaacgatga gtaatctgta gagtttgatt cagaccttct attttccgct tatccgtgcc
     5161 ccatctccca ttttccctca cccacgccgt caccgccttg tcatctttct gacaccttac
     5221 tatcttacaa atgtaacaaa aaagttattt ttctgtagtt cgagcatgtc atgttacccc
     5281 gcgagcataa aacgcgtgaa ttcgcgcatt cggtacaaca agagagataa acgatgaaac
     5341 cgttacatta tacagcttca gcactggcgc tcggactggc gttaatgggg aatgcacagg
     5401 cagtgacgac cattccgttc tggcattcta tggaagggga actgggtaaa gaggtggatt
     5461 ctctggccca acgttttaac gccgaaaatc cggattacaa aattgtaccg acctataaag
     5521 gcaactacga acagaattta agcgcgggga ttgccgcatt tcgtaccggc aacgctccgg
     5581 ctattttgca ggtttatgaa gttggcaccg ccaccatgat ggcatcgaaa gccattaaac
     5641 cggtatatga cgtgtttaaa gaggcgggga ttcaattcga tgagtcgcag tttgtgccga
     5701 cggtttccgg ttactactcc gacagcaaaa cgggccactt actctcccag ccattcaaca
     5761 gctcgacccc cgttctctat tacaacaaag acgccttcaa gaaagcagga ttagacccgg
     5821 aacaaccgcc gaaaacctgg caggatctgg cggactatgc cgcgaaactg aaagcctccg
     5881 gtatgaagtg cggctacgcc agcggctggc agggctggat ccaactggaa aactttagcg
     5941 cctggaacgg tctgccgttt gccagcaaaa acaacggctt tgacggcacg gacgcggtgc
     6001 tggagttcaa caagccggag caggtgaaac acatcgccat gctcgaggag atgaacaaga
     6061 agggcgactt cagctacgtc ggtcgtaagg atgaatccac cgagaagttc tataacggtg
     6121 attgcgcgat gaccaccgcc tcttccggtt ctctcgctaa tattcgtgag tacgccaaat
     6181 ttaactatgg cgtaggcatg atgccttacg acgccgatgc gaaagatgca ccgcaaaacg
     6241 ccattatcgg cggggccagc ctgtgggtga tgcagggcaa aaataaagaa acgtataccg
     6301 gcgtggcgaa gttcctcgac ttcctcgcga agccagaaaa cgctgccgag tggcatcaga
     6361 aaaccggtta tctgccaatc accaaagcag cgtatgacct gacccgtgag cagggctttt
     6421 acgagaaaaa cccaggggcg gataccgcga cgcgtcagat gctgaataag ccgccgttgc
     6481 cgttcaccaa agggctgcgt ctgggcaaca tgccgcagat ccgcgtgatt gtggatgaag
     6541 agctggagag cgtgtggacc ggtaagaaga caccacagca ggcactggat accgccgttg
     6601 agcgtggaaa tcagttgctg cgccgctttg agaaatcgac gaagtcttaa tcagtgtaat
     6661 gtcggatgcg tttcgcttat ctgacctggc atcgcgtgta ggccggataa gcgaagcgca
     6721 tccggcacag ttcaggaatt aaccgtaatg tcatcatccc gtccggtgtt ccgctcgcgc
     6781 tggctgcctt atctgctggt cgcgccgcag ctcatcatca ccgttatctt ttttatctgg
     6841 cctgcgggcg aagcgttgtg gtactcgcta caaagcgtcg atccgtttgg tttctccagc
     6901 cagtttgtcg gcctggataa cttcgtcgcg ctgtttcatg acagctacta tctcgactcc
     6961 ttctggacga cgataaaatt cagcaccttt gtcaccgtca gcggtttgct ggtgtcgctg
     7021 ttcttcgcgg cgctggtgga gtacatcgtg cgcggcagcc gtttctatca aaccttaatg
     7081 ctgctgcctt acgccgtggc tcccgccgtt gccgccgtat tgtggatctt cctgtttaac
     7141 cccggtcgcg ggctgatcac ccattttctc gcggagttcg gctacgactg gaaccacgcg
     7201 caaaacagcg gtcaggcaat gtttctggtg gtgtttgcct cagtatggaa gcaaatcagc
     7261 tacaacttcc tgttcttcta tgccgcgctg caatccattc cccgttcgtt gatcgaagcc
     7321 gcagccatcg acggtgcagg cccaattcgc cgcttcttta agattgcgct gccgcttatc
     7381 gctccggtga gtttcttcct gctggtggtg aacctggtgt atgccttctt cgacaccttc
     7441 ccggtgatcg acgccgccac gtccggcggg ccagttcagg ccaccacgac gctgatttat
     7501 aagatctacc gtgaaggctt taccggactg gatctggctt cgtctgccgc gcagtcggtg
     7561 gtgttgatgt tcctcgtcat cgtgctgacg gtggtgcagt tccgctatgt tgaaagcaag
     7621 gtgcgttacc aatgattgag aaccgtccgt ggctgacgat attcagccat accatgctga
     7681 tcctcgggat cgcggtgatc ctcttcccgc tgtacgtggc gtttgtcgcg gcgacgctgg
     7741 ataaacaggc cgtctatgcc gcgccgatga cgctcatccc cggcacacat ctgctggaaa
     7801 atatccacaa catctgggtt aacggggtag gcacgaatag cgcgccgttc tggcggatgt
     7861 tgcttaacag ctttgtgatg gcgttcagca ttacgctcgg caaaattacc gtctcgatgc
     7921 tctcggcatt tgccattgtc tggtttcgtt ttccgctacg taacctcttc ttctggatga
     7981 tttttatcac cctgatgctg ccggttgaag tacgtatctt cccgacggtg gaagtcatcg
     8041 ccaacctgaa gatgctcgac agctacgccg gtttaacgct gccgctgatg gcctcggcga
     8101 ccgccacgtt tctgtttcgc cagttcttta tgacgctgcc ggatgaactg gtggaagcgg
     8161 cgcggatcga cggcgcgtca ccaatgcgtt tcttttgcga catcgttttt ccgctctcca
     8221 aaaccaatct ggcggcgctg tttgtgatca cctttattta cggctggaac cagtatttgt
     8281 ggccgttgtt gattattacc gacgttgatc tcggcaccac cgtggcaggg atcaaaggga
     8341 tgatcgccac tggcgaaggc accacggaat ggaactcggt aatggcggcg atgttgttaa
     8401 cgcttatccc tccggtggtg attgttttag tgatgcagcg tgccttcgtg cgcggcctgg
     8461 tcgatagtga gaaataagat ggcaggactg aaattacagg cagtaaccaa aagctgggat
     8521 ggcaaaaccc aggtgattaa accactgacc cttgatgtgg cggatggcga atttatcgtg
     8581 atggtcgggc cgtctggctg cggaaaatcg acgctgctgc gcatggttgc cgggctggag
     8641 cgggtgacgg aaggcgatat ctggatcaac gaccagcgcg tgaccgaaat ggagccgaaa
     8701 gatcgcggga ttgcgatggt gttccagaac tacgcgcttt atccgcatat gagcgtcgaa
     8761 gaaaacatgg cgtgggggct gaaaattcgc ggcatgggca agcagcaaat agccgagcgc
     8821 gttaaagaag cggcgcgcat tctggagctg gatggcctgc ttaagcgccg cccgcgcgag
     8881 ctatccggcg gtcagcgtca gcgtgtggcg atggggcgcg cgattgtgcg cgatccggcg
     8941 gtgttcctgt ttgatgagcc gctctctaac ctcgatgcca agctgcgcgt gcagatgcgc
     9001 cttgagctgc aacagctgca ccgtcgcctg aaaacgactt cactctacgt tactcacgat
     9061 caggttgaag cgatgacgct cgcccagcga gtaatggtga tgaacggcgg cgttgccgaa
     9121 cagattggca caccagttga agtctacgaa aagcccgcca gcctgtttgt agcgagtttt
     9181 atcggcagtc cggcgatgaa cctgctgaca ggccgcgtga ataacgaagg cacgcatttc
     9241 gaactggacg gcggtattga gctgccgcta aacggtggct accgtcagta tgccgggcgt
     9301 aaaatgactc tcggcattcg cccggaacat attgcgctaa gctcgcaggc agaaggcggc
     9361 gtaccgctgg tgatggacac gctggagatc ctcggcgcag ataacctggc gcacggacgc
     9421 tggggcgaac agaagctggt ggtgcgactg gcgcatcagg agcgcccgac ggcaggcagc
     9481 acgctgtggc tgcatctgcc ggaaaatcag ctgcatcttt ttgatggtga aacaggacaa
     9541 cgagtatgag taactggcct tatccccgca tcgtcgccca tcgtggcggc ggtaaactgg
     9601 ccccggaaaa caccctggcg gcaatcgacg tgggggcaaa atacggtcat aagatgatcg
     9661 aatttgacgc gaagttatcg aaagatggcg agatcttcct gctccatgac gacaatctcg
     9721 aacgcaccag caacggctgg ggcgtcgcgg gtgaactgaa ctggcaggat ttactgcgcg
     9781 tggatgcggg cagttggtac agcaaagcgt ttaaaggtga gccgctgccg ttgctttcgc
     9841 aggtggcgga acgctgtcgc gaacacggga tgatggcgaa tatcgaaatc aaacccacca
     9901 ctggcaccgg gccattaacg ggcaaaacgg tggcgctggc ggcacgcgaa ctgtgggccg
     9961 atatgacgcc gccgctgctg tcatcgtttg agattgatgc tttagaagct gcacaacagg
    10021 ctgcaccgga actgccgcgc ggtttgttgt tggatgagtg gcgcgacgac tggcgagaac
    10081 tgaccgcgcg actgggctgc gtttctattc atctcaatca taagttgctt gataaagcgc
    10141 gagtgatgca gttgaaagac gccggattac ggattctggt ttataccgtc aataaacccc
    10201 agcgcgcggc agagttgctg cgctggggcg tggattgcat ctgcaccgat gcgattgacg
    10261 tgattggtcc gaactttacg gcccaatagt tttcaacgga atatcaggct gcggcgtgct
    10321 gggctgattc agcatgtcgc cgtttctcct ctccggcagc atatgctgct gattcaacga
    10381 actatccgga ttacggttgc tgtttaacat cccgccgttg gtgttgggca gcatttgctg
    10441 ccgggcggga tttcgctccc ccggctgcga ctgcaacacc cgctgagaat tattgtttat
    10501 ctggttttct aaatgctgct gttgcaactg cgtttgcgtt ttcagttgct gattcagcat
    10561 ccctttttgc tggatttgct gcgtttgcat ttgagtctgc atccgctgct ggctggggat
    10621 ctgatacccc ggctggttag ggttgttcag agtattaatg ggctgtgcaa agccgacaaa
    10681 cggcaggagt gccgtaagaa tcagaagtcg tttcatcgcg tatcctcctc tgaagatatc
    10741 ctttaagttt actcgcttcc cgacaaaacg atgattaatt cagagttata taccaggctt
    10801 agctggggtt gccccttaat ctctggagaa taacgatgat aaaaccgacg tttttacgcc
    10861 gggtggccat tgctgctctg ctctcaggaa gttgttttag cgccgccgcc gcgcctcctc
    10921 cgccacctgt ttcttatggc gtggaggaag atgtcttcca tccggtacgc gcgaaacagg
    10981 ggatggtggc gtctgtagat gctactgcca ctcaggtggg ggtggatatc ctcaaggagg
    11041 gcgggaatgc cattgatgcc gccgtggcag tggggtacgc gttggcggta acgcatccgc
    11101 aggcggggaa tctgggcggc ggtggtttta tgctgatccg cactaaggat ggcaatacaa
    11161 ccgctatcga tttccgcgaa atggcacccg ctaaagcgac ccgcgatatg ttcctcgatg
    11221 atcagggcaa cccggacagc aaaaaatcac tcacttcgca tctggcttcc ggcacaccag
    11281 gtacggtcgc tggtttctcg ctggcgttgg ataagtacgg cactatgccg ttgaacaaag
    11341 tggtgcagcc cgcgttcaaa ctggcacgcg atggttttat cgttaacgat gctttggcag
    11401 acgatctcaa aacctacggt agcgaagtgt tgccgaatca cgaaaacagc aaagctatct
    11461 tctggaaaga gggcgaaccg ctgaaaaagg gcgacacgct ggtgcaggcg aacctggcaa
    11521 agagcctgga gatgattgct gaaaacggcc cggacgaatt ctataaaggc acgattgcgg
    11581 aacagatcgc ccaggagatg cagaaaaacg gtggcttgat cactaaagaa gatttagcgg
    11641 cctataaagc ggtcgaacgc actccgataa gcggcgatta tcgcgggtat caggtttact
    11701 ccatgccacc gccatcctcc ggcgggatcc atatcgtaca aatcctcaat attctggaaa
    11761 acttcgatat gaagaaatac ggctttggca gcgccgatgc gatgcaaatc atggcagaag
    11821 cggagaaata cgcctacgcc gaccgctcgg aatatcttgg cgacccggat tttgtcaaag
    11881 tcccgtggca ggcactgacc aataaagcct atgccaaatc cattgccgat caaattgata
    11941 tcaacaaagc gaagccgtcc agtgagattc gccctggcaa gcttgcgcct tatgagagta
    12001 atcaaactac ccattactct gtagtggata aagacggtaa cgcggtggcg gtgacctata
    12061 cgctgaacac caccttcggt acgggtattg tcgcgggcga gagcggtatt ctgcttaata
    12121 accagatgga tgatttctcc gccaaaccgg gcgtaccgaa cgtttacggg ctggtgggcg
    12181 gtgatgccaa cgccgtcggg ccgaacaaac gcccgctgtc gtcgatgtcg ccgaccattg
    12241 tggtgaaaga cggtaaaacc tggctggtta ccggtagccc aggcggtagc cggatcatca
    12301 ctacagtgct gcaaatggtg gtgaatagca tcgattatgg catgaacgtc gccgaagcga
    12361 ccaatgcgcc gcgtttccac catcagtggt tgccggacga gctgcgtgtc gaaaaagggt
    12421 ttagcccgga tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaaagagg
    12481 cgatgggcag tacacaaagc attatggttg ggccggacgg tgagttgtac ggcgcatccg
    12541 acccgcgctc ggtggatgat ttaacggcgg ggtactaagg ttagcggccc tcttcgtggg
    12601 aagagggcta ttttgtcagg gcaagccgaa ggtagccttt tttatttcgt aatcctgtag
    12661 atattcttcc agcggctttg ccgttcccag agaatgaatt tcaccactgt ctttatcaat
    12721 aataaaaggt gcgttaccag ctaagcgcgc ggcctcatct ccagtttcga gaaattctcg
    12781 tgcttcgaaa cagaaatacc agccctggct aaagcgtcca tgtagagtaa tgacgaccgg
    12841 gagatctgca tcatcaaggt aat
//
LOCUS       DAPPNJ010000133        12795 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000133 DAPPNJ010000000
VERSION     DAPPNJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.134"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(112..1470)
                     /gene="plaP"
                     /locus_tag="RPM73_003123"
     CDS             complement(112..1470)
                     /gene="plaP"
                     /locus_tag="RPM73_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDX6521458.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1460..1522)
                     /gene="yoeI"
                     /locus_tag="RPM73_003124"
     CDS             complement(1460..1522)
                     /gene="yoeI"
                     /locus_tag="RPM73_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDX6521459.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1737..2666)
                     /locus_tag="RPM73_003125"
     CDS             complement(1737..2666)
                     /locus_tag="RPM73_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6521460.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2712..3536)
                     /locus_tag="RPM73_003126"
     CDS             complement(2712..3536)
                     /locus_tag="RPM73_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6521461.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3619..3873)
                     /gene="yoeB"
                     /locus_tag="RPM73_003127"
     CDS             complement(3619..3873)
                     /gene="yoeB"
                     /locus_tag="RPM73_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDX6521462.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3870..4121)
                     /gene="yefM"
                     /locus_tag="RPM73_003128"
     CDS             complement(3870..4121)
                     /gene="yefM"
                     /locus_tag="RPM73_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDX6521463.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4404..4454
                     /gene="hisL"
                     /locus_tag="RPM73_003129"
     CDS             4404..4454
                     /gene="hisL"
                     /locus_tag="RPM73_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDX6521464.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4430..4553
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4600..5499
                     /gene="hisG"
                     /locus_tag="RPM73_003130"
     CDS             4600..5499
                     /gene="hisG"
                     /locus_tag="RPM73_003130"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDX6521465.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5505..6809
                     /gene="hisD"
                     /locus_tag="RPM73_003131"
     CDS             5505..6809
                     /gene="hisD"
                     /locus_tag="RPM73_003131"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDX6521466.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6806..7876
                     /gene="hisC"
                     /locus_tag="RPM73_003132"
     CDS             6806..7876
                     /gene="hisC"
                     /locus_tag="RPM73_003132"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDX6521467.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIEYYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7876..8943
                     /gene="hisB"
                     /locus_tag="RPM73_003133"
     CDS             7876..8943
                     /gene="hisB"
                     /locus_tag="RPM73_003133"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDX6521468.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8943..9533
                     /gene="hisH"
                     /locus_tag="RPM73_003134"
     CDS             8943..9533
                     /gene="hisH"
                     /locus_tag="RPM73_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDX6521469.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9533..10270
                     /gene="hisA"
                     /locus_tag="RPM73_003135"
     CDS             9533..10270
                     /gene="hisA"
                     /locus_tag="RPM73_003135"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDX6521470.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLELLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10252..11028
                     /gene="hisF"
                     /locus_tag="RPM73_003136"
     CDS             10252..11028
                     /gene="hisF"
                     /locus_tag="RPM73_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDX6521471.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11022..11633
                     /locus_tag="RPM73_003137"
     CDS             11022..11633
                     /locus_tag="RPM73_003137"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDX6521472.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQNLDLTTVIENLRKRHQ"
     gene            complement(11730..12710)
                     /gene="wzzB"
                     /locus_tag="RPM73_003138"
     CDS             complement(11730..12710)
                     /gene="wzzB"
                     /locus_tag="RPM73_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDX6521473.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVPDLQEALIGRFSSA
                     FSALAETLGNQKEPEKLTIEPSVKNQQLPLTVSYVGQTAEDAQMKLAQYIQQVDDKVN
                     QELEKDLKDNLALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNSK"
BASE COUNT         3196 a         3373 c         3293 g         2933 t
ORIGIN      
        1 caaattctct aaattagaac aaatggttat taatgaggat gtcgataaaa aagaataaaa
       61 aaaccggagc caaatattca ttcgactccg gtttattatt acaaaagatg attacgctac
      121 gtcttcgtac tgcggaaccg ggttacggaa actcttggtg acgcaagcca ggtaaatcag
      181 accgatagcc gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag
      241 cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag
      301 cgtcttatta cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacgaaggt
      361 gaacgccacc aacgcaccaa agttaatcag cgctgtagcc attaccaggt cgaagttgat
      421 tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac gccatttcgg
      481 gtgtacataa ccgaagaagc ttttcgggaa tacgccgtca cgacccatta cgtacatcag
      541 acgcgctacg cctgcatgcg ccgccatacc ggacgccagt acggtaatgg tggagaagat
      601 cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag gctgtgacgc
      661 atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa
      721 gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc
      781 tgcatctttg gtttcttccg acaggttgct gatgccgtca aagccggtaa aggagaagca
      841 caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc agaacggacg
      901 agtgctcgcc agagtaccag cgccttcacc ttcaaatacg ccataaacaa ccatgcccag
      961 aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgaagt tcgctacgga
     1021 tttcagacta cgcaggttaa aggcggtcat gaaggccacc agcgccacca caaacatcca
     1081 cgatgggatg gaaggcacta gagcttcaaa atagattttc gccagcagaa tgttgatcat
     1141 cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct
     1201 aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc gaaccagctt
     1261 cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac
     1321 atgaccatcc gtgaggcctg aaacgatacc aaaagtatca aacagcgtca tcggctgcat
     1381 ataggcaaga cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg
     1441 agaggtgttt ggagtaacgt tatgcgacat ggtcattctc ctttacggtg ataaccgtcg
     1501 cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt
     1561 agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa
     1621 aaaataaccg acgctgatga aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt
     1681 gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttaa
     1741 atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa
     1801 atttcatccc gccaccagcc gctggcaagc cctgcggtct gttcattcca gccaatccag
     1861 acgggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc actatcgaga
     1921 aaccgttgcg ccagataacg gggtaaatag ccgcagccga ggccgctaat ttgcagctcc
     1981 agcttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc
     2041 gtagaagcgc ctgcctgcgc agtatccccc accacaatcg cccgatatcg cttaataata
     2101 cgacggttta acggctcttc ttccagagct aacggatgat gaggcgcgac cgcaaagact
     2161 tgttccagat cgcccaaccg tgaaaaacca aactcactgg aagatggtgg ctcatgcatc
     2221 gcgccaacaa taatatccgc tcgcccctga gtcagggcat cccaggaacc accgagtacg
     2281 ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc aatcagtggc
     2341 gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct
     2401 tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgccct
     2461 ttctctaata acatttttcc ggtacgggtg aatttagcgc ggtgaccgct acgatcaagc
     2521 aattgaatat taaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc
     2581 ttatagagtt tggccgacgc cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc
     2641 atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgtatgcg gcgtcctgct
     2701 ggcgtgatcg tttactccag cggcattacc agcggatcgg gatactggta ttcaaatccc
     2761 agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt
     2821 ctgaactgcg gaggctccag ccccagtaaa cgggccatct gcggatagaa aacattacgc
     2881 gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac
     2941 agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca
     3001 ggcgcggttt ttccggcaaa gaagcgtccg ggatgacgtc ccggtccgac cagccccgca
     3061 agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc gagttcttct
     3121 aatactcgtc cactgttggt taccggatta cgcggggtag tttctttcac cgtgccttgc
     3181 gcgtcgccat agacagatgt tgagctggta aaaataatgc ggggaatacg atgggccagc
     3241 gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga
     3301 cgtgccggaa gcgtaatgac cagcgcatcg acatccatca gggcatccag atcgtcagaa
     3361 tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact cattcgggcc
     3421 gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt
     3481 gacatcgcca gcggcatgcc taaccaccct aacccgacaa ttgcgacctt tttcatccgt
     3541 tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca
     3601 ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca gtgaatcgtc
     3661 ggtaaccgcg tataccagac ggtgatcctc tgtaatgcgt cgggaccaga aacctgacaa
     3721 attatgtttc aggggttctg gcttcccctt accttcaaat ggcgttctgc gggtatcttt
     3781 gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt acagataatc
     3841 gtcccatgat tcctcagacc agattagttt cactcaataa tgtccttttc tgttcctttg
     3901 cctgatttca ggctatcgat tgagtccatc aatctccggg cgttagcggg ggaacgcagt
     3961 agataagccg tctcttccag cgagttgtat tcttcgagtg acatcagaac acaagcctct
     4021 ccattctgac gagtaataag gatcggggca tgatcttcaa cggctttcat cattgttgcc
     4081 gacaaattct gacgcgcttc gctgtagcta attgtacgca tgtcaatctc ctcttttgta
     4141 cagttcattg tacaatgatg ggcgttaatt aactatttat taattagttt gtagatcaag
     4201 gtattgtcag tgagacgaaa atccaggctt cgctattttt ggtgccgtca gcttagagga
     4261 cagtcctctt agccccctcc tttccccgct cattcattaa acaaatccat cgccataaaa
     4321 tatataaaaa agcccttgct ttctaacgtg aaagtggttt aggttaaaag acatcagttg
     4381 aataaacatt cacagagact tttatgacac gcgttcaatt taaacaccac catcatcacc
     4441 atcatcctga ctagtctttc aggcgatgtg tgctggaaga cattcagatc ttccagtggt
     4501 gcatgaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgcgg
     4561 ttgctaacgg ttcagacagg tttaaagagg aataacaaaa tgacagacaa cactcgttta
     4621 cgcatagcta tgcagaaatc cggccgttta agtgatgact cacgcgaatt actggcgcgc
     4681 tgtggcatta aaatcaatct tcacacccag cgcctgatcg cgatggcaga aaacatgccg
     4741 attgatattc tgcgcgtgcg tgacgacgac attcccggtc tggtaatgga tggcgtggtt
     4801 gacctcggga ttattggcga aaacgtgctg gaagaagagc tgcttaaccg ccgcgcccag
     4861 ggtgaagatc cgcgctactt taccttgcgt cgtctggatt tcggcggctg ccgcctttcg
     4921 ctggcaacgc cggttgatga agcctgggac ggcccgctct ccttaaacgg taaacgtatc
     4981 gccacctctt atcctcacct gctcaagcgt tatctcgacc agaaaggcat ctcttttaaa
     5041 tcctgcttac tgaacggttc tgttgaagtc gccccgcgtg ccggactggc ggatgcgatt
     5101 tgcgatctgg tttccaccgg tgccacgctg gaagcaaacg gcctgcgcga agtcgaagtt
     5161 atctaccgct cgaaagcctg cctgatccag cgcgatggcg aaatggaaga atccaaacag
     5221 caactgatcg acaaactgct gacccgtatt cagggtgtga tccaggcgcg cgaatcaaaa
     5281 tacatcatga tgcacgcacc gaccgaacgc ctggatgaag tcatcgccct gctgccaggt
     5341 gccgaacgcc caactattct accgctggcg ggtgatcaac agcgcgtagc gatgcacatg
     5401 gtcagtagcg aaaccctgtt ctgggaaacg atggaaaaac tgaaagcgct gggtgccagt
     5461 tcaatcctgg tcctgccgat tgagaagatg atggagtgat cgccatgagc tttaacacaa
     5521 tcattgactg gaatagctgt actgcggagc aacaacgcca gctgttaatg cgcccggcga
     5581 tctccgcttc tgaaagcatt acccgcaccg ttaacgatat tctcgataac gtgaaagcac
     5641 gcggcgatga ggccctgcgg gaatacagcg cgaagtttga taaaaccacg gttaccgcac
     5701 tgaaggtgtc tgctgaggaa attgccgccg ccagcgaacg cctgagcgac gagctaaaac
     5761 aggcgatggc ggtggcagtg aagaatattg aaaccttcca cactgcgcaa aaactgccgc
     5821 cggtagatgt agaaacgcag ccaggcgtgc gttgccagca ggtcacgcgc ccggtcgctt
     5881 cagttgggtt gtatattcct ggcggctccg ccccgctctt ctcaacggta ttaatgctgg
     5941 cgactccggc gcgtattgcg ggctgtaaaa aagtggtgct gtgctcaccg ccgccgattg
     6001 ccgatgagat cctttatgcg gcgcagctgt gcggtgtgca ggacgtgttt aacgtcggcg
     6061 gcgcacaggc cattgccgca ctggcgtttg gtacggaatc tgtgccgaaa gtggacaaaa
     6121 tcttcgggcc gggcaacgcc tttgtcaccg aagcgaaacg tcaggtgagc cagcgtctgg
     6181 acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt gttggtcatt gccgacagtg
     6241 gcgcaacgcc ggatttcgtg gcttctgatt tgctctccca ggctgaacac ggcccggact
     6301 cacaggtgat tttactgacg cccgctgctg atatggcgcg tcgcgttgcc gaggccgtcg
     6361 aacgccagtt ggcagaactg ccgcgtgccg aaaccgcccg ccaggcactg aacgccagcc
     6421 gcctgatcgt gaccaacgat ttagcgcagt gcgtagagat ctccaatcag tacggcccgg
     6481 agcacctgat catccagacc cgcaacgccc gcgatctggt cgatggcatc accagcgccg
     6541 gttcggtatt tcttggtgac tggtcaccgg aatcggcagg tgattacgcc tccggcacca
     6601 accacgttct gccgacttac ggttacaccg ccacctgttc cagcctcggg ctggcagatt
     6661 tccagaagcg catgaccgtg caggaactgt cgaaagaagg cttttctgcg ctggcttcaa
     6721 ccatagaaac actggccgcc gccgagcgcc tgaccgccca taaaaatgcc gttactttgc
     6781 gtgttaacgc ccttaaggag caagcatgag caccgtgact atcaccgatt tagcgcgtga
     6841 aaacgtccgc aacctgacgc cgtatcagtc ggcgcgtcgt ctgggcggta acggcgatgt
     6901 ctggctgaac gccaacgaat atcccacagc cgtggagttt cagctcactc aacaaacgct
     6961 caaccgctac ccggaatgtc agccgaaagc ggtgattgaa tattacgcgc agtatgcagg
     7021 cgtaaaaccg gagcaggtgc tggtcagccg tggcgcggac gaaggtattg aactactgat
     7081 tcgcgctttt tgcgaaccag gtaaagatgc catcctctac tgcccgccaa cgtacggcat
     7141 gtacagcgtc agcgccgaaa cgattggcgt cgagtgccgc acagtgccga cgctggacaa
     7201 ctggcaactg gacttacagg gcatttccga caagctggac ggcgtaaaag tggtctatgt
     7261 ttgcagcccc aacaacccga ccgggcaact gatcaatccg caggattttc gcaccctgct
     7321 ggagttaacc cgcggtaagg cgattgtggt tgccgatgaa gcatatatcg agttttgccc
     7381 gcaggcatcg ctggctggct ggctggcgga atatccgcac ctggctattt tgcgcacact
     7441 gtcgaaagct tttgctctgg cggggcttcg ttgcggattt acgctggcaa acgaagaagt
     7501 catcaacctg ctgatgaaag tgatcgcccc ctacccgctc tcgacgccgg ttgccgacat
     7561 tgcggcccag gcgttaagcc cgcaggggat cgtcgccatg cgcgaacggg tagcgcaaat
     7621 tattgcagaa cgcgaatacc tgattgccgc actgaaagag atcccctgcg tagagcaggt
     7681 tttcgactct gaaaccaact acattctggc gcgctttaaa gcctccagcg cagtgtttaa
     7741 atctttgtgg gatcagggca ttatcttacg tgatcagaat aaacaaccct ctttaagcgg
     7801 ctgcctgcga attaccgtcg gaacccgtga agaaagccag cgcgtcattg acgccttacg
     7861 tgcggagcaa gtttgatgag tcagaagtat ctttttatcg atcgcgatgg aaccctgatt
     7921 agcgaaccgc cgagtgattt tcaggtggac cgttttgaca aactcgcctt tgaaccaggc
     7981 gtgatcccgg agctgctaaa gctgcaaaaa gcgggctaca agctggtgat gatcactaat
     8041 caggatggtc tgggaacaca aagtttcccg caggcggatt tcgatggccc gcacaacctg
     8101 atgatgcaga tcttcacctc gcaaggcgta cagtttgatg aagtgctgat ttgtccgcac
     8161 ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa aactggtgga gcgttatctg
     8221 gctgagcaag cgatggatcg cgccaacagt tatgtgattg gcgatcgcgc gaccgacatt
     8281 caactggcgg aaaacatggg cattaatggt ttacgctacg accgcgaaac cctcaactgg
     8341 ccaatgattg gcgagcagct cactaaacga gaccgttacg cccatgtagt gcgcaacacc
     8401 aaagagacgc agattgacgt tcaggtgtgg ctggatcgcg aaggtggcag caagattaac
     8461 accggcgttg gcttctttga tcatatgctg gatcagatcg ctacccacgg cggtttccgc
     8521 atggaaatca acgtcaaagg cgacctctat atcgacgatc accacaccgt cgaagatacc
     8581 ggcctggcgc tgggcgaagc gttaaaaatt gccctcggcg acaaacgcgg tatttgccgc
     8641 tttggttttg tgctaccgat ggacgaatgc cttgcccgct gcgcgctgga tatctctggt
     8701 cgcccgcacc tggaatataa agccgagttt acctaccagc gcgtgggcga tctcagcacc
     8761 gaaatgatcg agcacttctt ccgttcgctc tcttacacca tgggcgtgac gctacacctg
     8821 aaaaccaaag gtaaaaacga tcaccaccgt gtagagagcc tgttcaaagc ctttggtcgc
     8881 accctgcgcc aggccatccg cgtggaaggc gataccctgc cctcgtcgaa aggagtgctg
     8941 taatgaacgt ggtgatcctt gataccggct gcgccaatct gaactcggtg aagtctgcca
     9001 ttgcgcgcca cggttatgaa ccaaaagtca gccgtgaccc ggacgtcgtg ttgctggccg
     9061 ataaactatt tttacccggc gttggcaccg cacaagcagc gatggatcag gtgcgtgagc
     9121 gcgagctgtt tgatctcatc aaagcctgta cccaaccggt gctgggcatc tgcttaggga
     9181 tgcaactgct ggggcggcgc agcgaagaga gcaacggcgt cgatttgctg ggcatcatcg
     9241 acgaagacgt gccgaaaatg accgactttg gtctgccact gccgcatatg ggctggaacc
     9301 gcgtttaccc gcaggcaggc aaccgcctgt ttcaggggat tgaagacggt gcgtactttt
     9361 actttgttca cagctacgca atgccggtca atccgtggac catcgcccag tgtaattacg
     9421 gcgaaccgtt taccgcagcg gtacaaaaag ataacttcta cggcgtgcag ttccacccgg
     9481 agcgttctgg tgccgctggc gctaagttgc tgaaaaactt cctggagatg tgatgattat
     9541 tccggcatta gatttaatcg acggcaccgt ggtgcgtctc catcagggcg attacggcaa
     9601 acaacgtgat tacggtaacg acccgctgcc gcgcttacag gattacgccg cacaaggtgc
     9661 cgaagtgttg catctggtgg atctgaccgg ggcaaaagat ccggcaaaac gtcaaatccc
     9721 gctgattaaa actctggtcg cgggcgttaa cgttccggtg caggttggcg gcggcgtgcg
     9781 tagcgaagaa gacgtggcgg cgttactgga agcaggcgtt gcgcgtgtgg tggttggctc
     9841 caccgcggtg aaatcaccag aaagggtgaa aggctggttt gaacgcttcg gtgccgatgc
     9901 cttagtgctg gcgctggatg tccgtattga cgagcaaggc aacaagcagg tggcagtcag
     9961 cggctggcaa gagaattcgg gcgtgtcgct ggaactactg gtggaaacct atctgcccgt
    10021 cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac ggcacgctgg caggttctaa
    10081 cgtctcttta tatgaagaag tgtgcgccag atatccgcag gtggcatttc agtcctccgg
    10141 cggtattggc gacattgatg atgtggcggc cctgcgtggc actggtgtgc gcggcgtaat
    10201 agttggtcgt gcattactgg aaggtaaatt caccgtgaag gaggccatcg catgctggca
    10261 aaacgcataa ttccatgtct cgacgttcgt gatggtcagg tggtaaaagg cgtacagttt
    10321 cgcaaccatg aaatcattgg cgatatcgtg ccgctggcaa aacgctacgc tgaagaaggt
    10381 gcagacgaac tggtgttcta cgatatcacc gcttccagcg atggccgtgt ggtagataaa
    10441 agctgggtat ctcgcgtggc ggaagtgatc gacattccgt tctgcgtggc gggtgggatt
    10501 aagtctctgg aagatgccgc gaaaattctt tcctttggcg cggataaaat ttccatcaac
    10561 tcgcctgcgc tggcggaccc gacgttaatt actcgcctgg cggatcgctt tggcgtgcag
    10621 tgtattgtgg tcggtattga tacctggtac gacggcgaaa ccggtaaata tcatgtgaat
    10681 caatataccg gcgatgaaag ccgcacccgc gttactcagt gggaaacact cgactgggta
    10741 gaggaagtgc aaaaacgcgg tgccggagaa atcgtcctca acatgatgaa tcaggacggc
    10801 gtgcgtaacg gttacgacct cgaacagctg aaaaaagtgc gtgaagtttg ccacgtcccg
    10861 ctgattgcct ccggtggcgc gggcaccatg gaacacttcc tcgaagcctt ccgcgatgcc
    10921 gacgttgacg gcgcgctggc agcttccgta ttccacaaac aaataatcaa tattggtgaa
    10981 ttaaaagcgt acctggctac acagggcgtg gagatcagga tatgttaaca gaacaacaac
    11041 gtcgcgaact ggactgggaa aaaaccgacg gacttatgcc ggtgattgtg caacacgcgg
    11101 tatccggtga agtgttaatg ctgggctata tgaacccgca agctctggac aaaaccatag
    11161 aaagcggcaa agtcaccttc ttctcgcgca ctaaacagcg actgtggacc aaaggcgaaa
    11221 cgtcgggcaa tttcctcaac gtagtaaata ttacgccgga ctgcgacaac gacacgttac
    11281 tgttgctggc gaatcccatc ggcccgacct gccacaaagg caccagcagc tgcttcggcg
    11341 acaccgctca ccagtggctg ttcctgtatc aactggaaca actgctcgcc gaacgcaaat
    11401 ctgccgaccc ggaaacctct tacactgcca aactgtatgc cagcggcacc aagcgcattg
    11461 cgcagaaagt gggcgaagaa ggggtggaaa ccgcactggc agcaacggtg catgaccgct
    11521 ttgaactgac caacgaagca tctgacttga tgtatcacct gctggtgctg ttgcaggatc
    11581 agaatctgga cttaacgacg gtgattgaga acctgcgtaa acggcatcag tgagttgcgg
    11641 ggtaagcgga tgcgatattg tcgtcgcatc cggcaaaaaa aacgggcaag gtgtcaccac
    11701 cctgcccatt ttctttaaaa ccgaaaagat tacttcgagt tgtaattacg cagagcatta
    11761 cgccccaaca caatccccgc gccaaccatg ccgcccagca gcaccgccag aatcaaggta
    11821 attgcctttt tcgggctatc gcgacgaata ggtaacgtcg gtttcatcac ataacggtaa
    11881 gcatgaatat caagatcgtc aacctttaag ttttcgatat ccagcaggtt ttgacgagtc
    11941 tgatagtagt ttggcgaaaa caccaacgga cgagtcgcct catgcttaat catcgactcc
    12001 agcgcttcgc tccccaaaag gaacaacgta tcttgtgtga tatcttcgcc agtctgttga
    12061 atctgcggtt ttgtcacctg cgcctgattc gcatactgca atgcttcctg aatctgacgg
    12121 atacgcagat ctttctgctc ctgcgcaacc acttcctgag ttcttaaaga gtcctgcaag
    12181 tttttccgtc ccaaagcaag gttgtccttg agatcctttt ctagctcttg atttacttta
    12241 tcatcaactt gctgaatgta ttgggccaat ttcatttgtg cgtcctctgc agtttgccca
    12301 acataagaaa cagtcaatgg taattgctgg ttctttacag aaggttcgat ggtaagtttt
    12361 tctggttctt tctgattacc cagcgtttct gctaatgcag agaaggcaga actgaagcga
    12421 ccaattaacg cctcctgtaa atccggcact ttaggtgcag cctgaccata gataacattc
    12481 atggcattgt tatagccagc aatttgcccc acgtcaggct gagtgataat tgctgttgac
    12541 gtccatttct ccttcgctac cgccaaatac ccaatagcca gggcaatagc aacaatgaca
    12601 gagatgataa ttgtcatctt gccgcgccac aactgcacca gtaaatcaat caaatcaatc
    12661 tgttccgggt catggttttg cccagaaaca ttattatttt ctactctcat cattacccta
    12721 acggacgaaa taattttaag tcgccatagt ttagcgataa tcgccatgaa tgctatagga
    12781 taaatgataa aaata
//
LOCUS       DAPPNJ010000134        12725 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000134 DAPPNJ010000000
VERSION     DAPPNJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.135"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..988
                     /gene="galE"
                     /locus_tag="RPM73_003139"
     CDS             <1..988
                     /gene="galE"
                     /locus_tag="RPM73_003139"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDX6521474.1"
                     /translation="IGSHTVLALQERNDDVVVLDNLSNASQTSLERVAELTGKKPIFY
                     KGDVMDRHLLKKIFASHNITDVIHFAGLKSVSESIKDPLSYYQNNVTGTLVLLEEMRA
                     AGVNSFIFSSSATVYGNPDRVPLNENSRTGGTTNPYGTSKYMVEQILEDFSRAQPEFR
                     ITCLRYFNPVGAHPSGRIGEDPNGIPNNLVPYIAQVTIGRLEVLSVYGNDYPTPDGTG
                     VRDYIHVMDLAHGHLAALDNKDKGDAYKVFNLGTGIGYSVLDLVNAFEKAAQTKINYR
                     FAPRRGGDIAECWSDPSRARRELGWQATRTIEEMMRDTWNWQKNNPNGYRSV"
     gene            complement(1036..2439)
                     /locus_tag="RPM73_003140"
     CDS             complement(1036..2439)
                     /locus_tag="RPM73_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HDX6521475.1"
                     /translation="MKHQWMTAIFCLVCPLSSMAAITTVMPTALTGPLTNPGMGVASF
                     HQGYGEKLPLTEYPDTGIEYERFYWRDLEPEEGKYHFARVDDAFKYAAAHRPAMNVGL
                     RFMALAEPGSGSRIPDWLIKKGIKGTWTADRKSFIPDLADPLFITYSQRLLQALGQRY
                     DGNDNLAFVDIGMVGSWGEWHNSNFPELKPLLERNTTEELDRFVNMHFSAFPKTPKVM
                     LISGGNSLVNAVRKGAGWRADCWGDLRMFSSTWNHMADDYPQRLEAAQRAYPGFNDAW
                     KRAPVNLEICGYMAQWQRDQHYTREEVQRIFDWALAHHASTLNLKSRVIPGEYRQIVD
                     NALTKIGYRFRLVSLSHESVGQPGTALTLNSRWVNEGVAPIYLPYTLSFRLVDASGKP
                     VASGESKEDLLRWLPGEYTVDFLLDMPRTMPGGSYFIEVAIVDKSGAPRIRLANEGQQ
                     SSNWYRISKVTVLSSTQ"
     gene            complement(2462..2869)
                     /locus_tag="RPM73_003141"
     CDS             complement(2462..2869)
                     /locus_tag="RPM73_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521476.1"
                     /translation="MTRSEQRPLVIDRFYEALDEQTAEQLTAQQKAEVEKAIIAITLA
                     SRHRVDFRRSFPFFGKRFYLVFLMGRDMRKRFRHESTLSSLLAAFLLLSGLLFLTFCV
                     LLTLYMIKSALGIDVFHDYHVGIWDWWLSLKNQ"
     gene            complement(2872..3984)
                     /locus_tag="RPM73_003142"
     CDS             complement(2872..3984)
                     /locus_tag="RPM73_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDX6521477.1"
                     /translation="MSQKITVLGTRGIPDVQGGVETHCQNLYPAIKKQFDMDICVIAR
                     SPYVSYKQTYYKNVETYSLWAPKKRSLEAIVHSFLATLRTCFDGSDIVHVHAIGPGLL
                     VPLLRVLGKKVVFTHHGPDYDRQKWGRLAKRVLQLGEKVAVKYANEVIVISEVINQLI
                     RTKHCRDDAHLIYNGVNLPLPLKEETVRTVLGRYALQPQNYLVVVGRFVEEKGMHDAI
                     AAHRKLGLTMPLVLVGDADHPTEYSVRLKKMAADTPNVIMTGFLKGEELQAIFSQARL
                     FLMPSYHEGLPIALLEAMAYSLPAVVSDIPANLEVKLPPESYFEVGNVDALAQKIAAL
                     VSSQRIDYSAWLKNYDWQVIARKTASVYHSLANKKG"
     gene            complement(3986..5197)
                     /locus_tag="RPM73_003143"
     CDS             complement(3986..5197)
                     /locus_tag="RPM73_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDX6521478.1"
                     /translation="MKILLVNKFFFIKGGAETVYFQERNWLKAAGVDVVDFSMLHEKN
                     FPSEYADTFVRNVDYHKESTLLGEVKTASNFIHNAEACRKMRTLLQRERPDIVHFHNI
                     YHQLTPALIKVARNFGCKTVLTAHDYKIACPAYSMLRDGKVCDACLTGTVFNAFRYRC
                     QEGSATKSLLLSLEATWQSIARNYHMLDVIISPSEFLKGILRRKLPHSRIDVIVNGVD
                     DDPATDKTADKGYLLYVGRLSREKGVATLPLAHQKMRNRAPLKVVGHGPLYDELVANY
                     PDVEFLGYVQQGEALNTLIKEARAVILPSECYENCSMSVLEAMSFAKPVIGSRIGGIP
                     EQIRDGIDGVLFEPGNVQDLANAMDYMIDSPEKARVMGLSARERLREKYTLQKHMETL
                     TALYKEILSWS"
     gene            complement(5211..6200)
                     /locus_tag="RPM73_003144"
     CDS             complement(5211..6200)
                     /locus_tag="RPM73_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX6521479.1"
                     /translation="MNNKPFISVSIKTLNESECIEKTIDSIRQQLRGYPHKIIVADSL
                     STDNTQQLAGDKGVMVVSLTEPGDRCCGVGHQLGYLYSEGDYILLMDGDMELEPGFID
                     RAVTFLEANSEYAGVAGTVEMDEAANYEFISRKQRLDTIYPVGDCDHLGGGGLYRRSA
                     IRKIGYLTNRNLHAYEEAELGLRLLEHGYKLHRLNIPYFRHTSYTLPTFKMLRYRWRS
                     GYYQGMGEILRSAWGKPYFSTVVKMVKSEVVFLLYLMLLVCSVFTLNMDIVGVALLPL
                     LVFIVLKTIKNRSLVNGLYSAMNMTIRAAGLLKGLMQPMRDPIVPPGNKIIHR"
     gene            complement(6190..7575)
                     /locus_tag="RPM73_003145"
     CDS             complement(6190..7575)
                     /locus_tag="RPM73_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDX6521480.1"
                     /translation="MTLNTGFYLQIYTFITLILCGLVQYFTGMQAVLWLPFCLALVMV
                     GLLVMQTRDGTQGLDDQETIILAVYCSFLALVGLSTLIQGGPVVAIIAFKNEIGLSLV
                     MICLLLGFCRESQIYRVTRCLYWVFYAQIPVMFYQVFFVVPQRVALLGEDEKWDSVVG
                     TFGGDPMGGGNTAAMGLFCLLIMLLKLSEYKHGLTTIKSLVLHIILGLGLCIIGEVKF
                     VILLSPLFLAWVWVTPGYVKDISKVNLKALLVIILGMVLLITLAIVILASFYSAAFGT
                     DPTQSSLSVFWESLSYIFDPNYILSTGELGRFTTLFFWWENNDLLGISGTLFGYGLNA
                     TNSGSSVSPGFLNLIYNLILDSTSLSMLLWEVGVIGTVLFIGLFIFILKVTQAKPVLR
                     LEQLDQQDLQLVSFAPAYNAFVISCLLSIPYSQILMITPMLQFLFYLSLGCSLVIRRT
                     VLRSVESLYEQ"
     gene            complement(7572..8783)
                     /locus_tag="RPM73_003146"
     CDS             complement(7572..8783)
                     /locus_tag="RPM73_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDX6521481.1"
                     /translation="MSLLKSASTIAGSSVISQLIGAFSIWLISYKYDLAEVGLYALNY
                     SIAVIGAQVCTFASQLLIPKQSEEELTQNVVFCLLQSAILALPYALLTAWLFHQNVLF
                     LYLLSLSNAWVLISENLSLRTGNFRFLIFQRISVSVVVVLSVVLTNQVQMFYWTWASG
                     MMLLTISCIARSFNFRTVTAQHLSLKSNLVFFRTHFHHISRVGSAEVLAMANNNLPIM
                     LINFWFSALTAGYFSVVSRFCLSPVTIVGNAVRNTIFSKWSIDFRNNTFNYPEYQRVR
                     FLLMVLGVICTLGVFIFYPIVMHLGFGEDWINSIDTSRYMLPYLFPALAVSPLTVIEL
                     IFGSHRYFLRIQLEQLAIVLIAFVVTPYFYNDYATSVIIFSVLTFIRYAFIYLAMNKR
                     ATLLKNNPVMP"
     gene            complement(8780..10912)
                     /locus_tag="RPM73_003147"
     CDS             complement(8780..10912)
                     /locus_tag="RPM73_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDX6521482.1"
                     /translation="MKLSIVENGKKREETVDVSRFAKEIKKNACKIVLAGIISGAVAY
                     PLISMLSSKYVSTATVLLKAQADNVSPFPQVEDFDSTRTGYYETQYALMQSRIVLEKA
                     VRELKLDQNPDFIGKKADEKASNSEDAEQQRIERALNTLQKNLTVSGIRTTNLATVSY
                     ESTSPQLSSEIANGVAQAFIDYTLDQKRLKTEKAREVNLQKMEEVQKEIAQQKADIDN
                     FLAKEGLLTFRGIDGFETEQLSIVTNRLADATQRRIAAESLEKAVSAGGRVSLDNIIS
                     LPTISNHAQIQDLRIAMIQAQRSLYELQKSYGPKHAKILEAQAQVKAIQDQMGVVLSE
                     LKKGIHQQYLAALADEKDYQAQLDQQKEIFQKLAEKRSLYNSQKLSLDKLEDLYKTLY
                     QRTQELSLSGINADAVLYDPAVPAVKPSKPNKALLLVMVVALAMAFFFMYVIVKAAMD
                     NSIRTLGQVTKRLGVVSLGEIRRIAGAGDRAQVRDLITRNPLNADIIHSIRTQILLDN
                     RPQQVLAISSAKQGEGRSLLASLLANSFSFDQKTLLLDLDFFNRDGLSAEFSTSTSAG
                     VAELLRGEVTLDAARITLSDTLDFLPRGKANASSLLMLSSERFEPLIRDLRNRYQRII
                     VDVSAVSQSQDIELISRVVDGVVFVVQAGAASVETLRAALAKVDANQEVVMGAVLNLV
                     EEKNLQTKESLRSLNITTDELMNTTGRL"
     gene            complement(10915..11472)
                     /locus_tag="RPM73_003148"
     CDS             complement(10915..11472)
                     /locus_tag="RPM73_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDX6521483.1"
                     /translation="MKVINLCTFLLCSILLVGCSSTRPPLTDDASTVTESYILGAGDT
                     VNIMVYGEPEMTMKFIIDKSGAITFPYIGKLMLKGKTAEQVGKEITKKLRGHYLQNPM
                     VTVSIAEFRKFYVSGEVRNPNGYAYEPGMTVEKSIALAGGFTDRADRKDINIRLSGNN
                     ELLENVALTHSVRPGDVVIIGMGFF"
     gene            complement(11474..12724)
                     /locus_tag="RPM73_003149"
     CDS             complement(11474..12724)
                     /locus_tag="RPM73_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDX6521484.1"
                     /translation="MLQPVWAEPIPKSHTGIAGVDFQSDVAANYGYSDNITFQPHSSK
                     EKDSVFQSITPTLSMVGERFQDKYLLMYSGDYRRYDKDSADNYNDHFFRFNGAWRYGL
                     KHGLTLNLEDSLGHEERGRGITEGFVQEQFRQFGITSPLSPHFVNSELRYSYGAPEGR
                     GKAELALQYKKLRFGKTGGVRNADVDFYNYLLEQEWYENSLIAEVYDQYTANTRFRYS
                     FITNQRRYDRLSEKDSNEYYLRYGVKSQLSDKTNIDMNVAWLYKTFENNANARNFNGL
                     NWDIQGEWKPLKQSVVTLHTSQNIKDPSEVGGYILFTKYGVSYQHFWLGDRFSTTLDY
                     SLSREDYKKQDKNRRDRNGVFTMKMSYDYTPSVNVELKYLLNKLDSNKNTDSFYIGPN
                     DEREVTRTLGYDNSMIMLTAKVQI"
BASE COUNT         3427 a         3313 c         2844 g         3141 t
ORIGIN      
        1 tataggttcg catactgtgt tggctctcca ggaaaggaat gatgacgttg tagttcttga
       61 taatctttct aacgcttcgc aaacctctct ggaaagagtt gcagaattaa ccgggaagaa
      121 acccattttc tataaaggcg acgtgatgga cagacatctt ctgaagaaga tttttgcgtc
      181 gcataatatt actgatgtga ttcattttgc aggtctaaaa tccgtttctg aatcgataaa
      241 agatccgctc tcttattacc agaataacgt cactggcaca ctggttctgc tcgaggaaat
      301 gcgtgcggcc ggcgtaaaca gctttatatt cagttcttcg gccaccgtgt acggcaaccc
      361 ggatcgcgta ccgctcaatg aaaactcccg tacgggcggt accactaatc catatggcac
      421 atccaaatat atggttgaac agattctgga agatttttcg cgagctcagc ctgaattccg
      481 tattacctgt ctgcgttact tcaacccagt cggggcgcat ccgtcaggac gtattggtga
      541 agatcccaat gggatcccga acaatctggt cccttatatt gctcaggtga ccattggcag
      601 gctggaagta ctatccgtgt acggtaatga ttaccccact ccggacggta cgggcgtacg
      661 cgactatatt catgtaatgg atctcgccca tggccatctg gcagcactgg acaataagga
      721 taaaggtgac gcgtataaag tctttaatct tggcaccggt atcggctatt ccgtactcga
      781 ccttgtgaac gcgttcgaaa aagcggcgca gacaaaaatc aattaccgct ttgcaccgag
      841 gagaggagga gatattgcag aatgctggtc agatccgtcc cgagcacggc gggaattggg
      901 ctggcaggcc acccgcacta ttgaagaaat gatgcgtgat acctggaact ggcagaaaaa
      961 taacccgaac ggctatcgca gcgtctgatc ctcagtttag atcctcttca cgatgggcca
     1021 taaacgccca tcgtttcatt gcgtcgatga cagaacagtc acctttgaaa tccggtacca
     1081 gttgctgctt tgctgcccct cattagccag ccggatcctt ggcgcccccg atttatcgac
     1141 aatcgccacc tctataaagt aactgcctcc cggcattgtc cgcggcatat ccagcagaaa
     1201 atccaccgta tactcaccgg gcaaccagcg gaggagatct tccttcgatt ctccgctggc
     1261 tacaggtttc ccggaagcat ccacgaggcg gaatgacagc gtataaggaa gataaatcgg
     1321 cgcgacgcct tcgtttaccc aacggctgtt gagcgtcagg gcggtgcctg gctgccctac
     1381 tgactcatga cttaacgata ccagacgaaa acggtagccg attttagtca gcgcattatc
     1441 gacaatctgc cggtactcac cggggataac acgtgatttc agattaagcg tgctggcatg
     1501 gtgtgccagt gcccaatcaa atatcctctg tacttcctcg cgcgtatagt gctgatcgcg
     1561 ttgccactgc gccatatacc cgcatatttc cagattcacc ggtgcgcgtt tccatgcatc
     1621 attaaagcct ggataggctc gctgcgctgc ctcgagccgc tgagggtaat catcggccat
     1681 atgattccac gttgacgaaa acatgcgtaa gtcgccccag cagtccgcac gccatccggc
     1741 gcccttacgc acagcattca ccaggctatt accgccgctt atcaacatga ctttgggcgt
     1801 ctttggaaac gctgaaaaat gcatattgac gaaccgatcg agctcctccg tcgtgtttct
     1861 ttccaacaga ggtttcagtt cagggaagtt gctgttatgc cactctcccc atgatcccac
     1921 cataccaata tccacaaacg ccagattgtc attgccatcg tagcgttgac ctaatgcctg
     1981 gagcagtcgt tgactatagg taataaaaag cgggtcggct aagtcaggaa tgaagctctt
     2041 acgatccgca gtccatgtac ctttgatgcc tttttttatc agccagtcgg ggatcctgga
     2101 gccgctaccc ggctcggcca gtgccataaa tcgcagacca acattcatgg ccggccgatg
     2161 tgccgctgcg tacttaaaag cgtcatccac ccgcgcaaaa tgatacttgc cttcctcggg
     2221 ctccagatcg cgccagtaaa aacgttcgta ctcaatgccg gtatcggggt attccgtcag
     2281 cggcagtttt tcaccataac cctgatgaaa actggccacg cccatgcccg gattggttaa
     2341 aggccccgtc agcgcggttg gcattaccgt cgttattgcc gccatcgacg acagtgggca
     2401 caccaggcag aatatggccg tcatccactg atgtttcatc actctctcaa acagatcttt
     2461 gctactgatt ttttaggctt aaccaccagt cccagatacc aacatgataa tcatggaaca
     2521 catcaatgcc taaagcggat ttgatcatat acagcgtcag caggacacaa aacgttagaa
     2581 acagaagccc agataacagc agaaaagccg ccagcagact ggacaacgtc gactcatggc
     2641 ggaatcgctt acgcatatcc ctgcccatca gaaataccag ataaaaacgc ttgccgaaaa
     2701 acggaaagct tcggcgaaaa tccaccctat gccttgaggc cagcgtgatc gcaatgatcg
     2761 ccttttcaac ctcggctttc tgctgcgcag ttaattgctc agcagtttgc tcgtcaagcg
     2821 cttcatagaa ccggtcaatg acaagaggac gttgttcaga acgggtcaaa attaaccttt
     2881 tttatttgct aaggaatggt agacactggc ggtttttctc gcgatcacct gccagtcgta
     2941 atttttcagc caggcgctgt agtcaatccg ctgtgaggaa accaacgctg ctattttttg
     3001 cgccagagcg tcgacgttgc cgacctcgaa atacgattct ggcggcaatt ttacttcaag
     3061 attcgcagga atatcactga ccacggcggg cagtgaatag gccatcgctt cgagaagcgc
     3121 tatcggtaac ccttcatggt atgaaggcat caaaaacagc cgcgcctgag aaaagatagc
     3181 ctgcaattcc tcacctttga ggaaccccgt catgatgacg ttcggcgtat ctgcagccat
     3241 ctttttaagg cggacgctat attccgtggg atgatcggca tcacccacca ataccagcgg
     3301 catcgtgagc cccagtttgc ggtgggcagc aatcgcatca tgcatacctt tttcttccac
     3361 aaaccgccca acgacaacca ggtaattttg cggctgcagc gcgtaacgtc ccaacaccgt
     3421 gcgcacagtc tcttccttta acggcaacgg taaattgacg ccgttgtaga tcaagtgtgc
     3481 atcatcacga cagtgttttg tgcgtatcag ttgattaatc acctctgaaa taacgatcac
     3541 ttcattggca tacttaacag ccactttctc tcccagttgc agcacccttt tagccagacg
     3601 cccccatttc tggcgatcgt aatctggacc atggtgggta aacaccacct tctttcctag
     3661 cacacgcagc agtggcacca gaagtccggg tccgatggca tgaacgtgca caatatcaga
     3721 accatcgaaa caggttctta acgtggctaa gaaggaatgg acaatcgctt ccagcgatcg
     3781 cttcttcgga gcccatagag agtatgtttc aacattttta taatacgtct gtttatagct
     3841 gacgtaggga gagcgagcga taacgcagat atccatatca aactgctttt ttatagccgg
     3901 gtaaagattc tggcagtgtg tttccacacc accctggaca tccggtatcc cgcgtgtacc
     3961 gagaaccgtg attttttgtg acatatcagg accagctcag aatttcctta tacaacgcag
     4021 ttagcgtttc catatgcttt tgaagcgtat atttttcgcg cagacgttcg cgagcggata
     4081 gccccatgac gcgggccttt tccggggaat caatcatgta atccattgca ttggcgagat
     4141 cctgcacatt cccgggttca aacagaactc cgtcgatacc gtctcgaatt tgttccggaa
     4201 tacccccaat ccgcgaacca atgaccggtt tagcgaagga cattgcctcc agcaccgaca
     4261 tggagcagtt ctcatagcat tcggaaggaa ggatcaccgc gcgcgcctct ttaatcagcg
     4321 tattaagcgc ctccccctgc tgtacgtagc ctaaaaattc gacatccggg tagttggcca
     4381 ccagctcgtc gtagagtgga ccatggccca ccacctttaa cggcgcacgg ttgcgcattt
     4441 tctgatgtgc cagaggcaat gtggcgaccc ctttttcacg gctgagccgg ccaacataca
     4501 gcaggtagcc tttatcggcg gtcttgtctg tcgcagggtc gtcatcgaca ccgttgacga
     4561 tcacatcaat ccgcgaatgc ggcaatttgc gccttaatat ccctttcagg aattcactcg
     4621 gcgaaattat cacatcgagc atgtgataat ttctggcaat cgactgccag gtcgcttcca
     4681 aagaaagcag taaactcttc gtcgcagaac cttcctggca gcggtaacga aaagcgttga
     4741 ataccgtccc ggtcaggcat gcatcgcaca ccttaccgtc cctcagcatg gagtaggccg
     4801 gacaggcgat tttatagtcg tgagcggtga gaaccgtttt acaaccaaaa ttgcgggcca
     4861 ccttaatgag ggccggggtt aattgatgat agatattatg aaagtgcaca atgtctgggc
     4921 gttcacgctg caataatgtc cgcatttttc ggcaagcctc tgcattgtga ataaaattac
     4981 tcgccgtttt cacctcgccc agaagggtgc tttctttgtg gtaatcaaca ttacggacaa
     5041 aagtatcggc atactcggac ggaaagtttt tctcatgcag catggagaaa tccaccacgt
     5101 cgacacccgc tgctttcagc cagtttctct cctgaaaata aacggtttcc gcacctcctt
     5161 taataaagaa aaacttgtta actagtaata ttttcatttc gtcaactcca ttaacgatga
     5221 atgattttat tgccaggcgg tacaatcgga tcgcgcatcg gttgcatcag ccctttcaat
     5281 aatccagcag cgcgaatggt catattcata gcgctgtata gtccattaac cagcgaacga
     5341 ttcttaatag ttttaagtac gataaataca agtagcggca gcagcgcaac gccgacaata
     5401 tccatattca gcgtaaatac tgaacatacc aacagcatca aatacagtaa aaaaaccact
     5461 tcgcttttta ccattttcac tacggttgaa aaatacggtt ttccccaggc gctacgtaat
     5521 atttcgccca taccctggta ataaccactc cgccagcggt agcgcaacat tttgaatgtt
     5581 ggcaacgtgt acgacgtatg gcggaaataa ggaatattca ggcgatgcaa tttatagcca
     5641 tgttccaata aacgaagccc cagttcagcc tcttcatacg cgtgtagatt gcggttggtc
     5701 agataaccaa tttttctgat tgcagaacga cggtataacc cgccgccacc taaatggtcg
     5761 caatccccaa ctggatagat cgtatcaagg cgctgtttac gggagataaa ctcatagttt
     5821 gcggcctcat ccatctccac cgtcccagcc acgccagcat actctgaatt tgcttccaga
     5881 aaggtcaccg cgcgatcaat aaagccgggt tcaagctcca tatcaccgtc catcagcaga
     5941 atgtaatccc cttcgctgta gagataaccg agctgatgtc caacgccgca acagcgatcg
     6001 cccggctcgg tgagcgaaac caccattacc cctttgtcac ccgccagttg ttgggtgtta
     6061 tccgtggaca ggctatcggc aacgataatt ttatgtggat aacctctaag ctgctgacgg
     6121 atgctatcga tagttttctc aatacactca gattcattga gcgtttttat gctcactgaa
     6181 ataaacggtt tattgttcat acaatgactc aacactacga agaacggttc gacgaattac
     6241 cagactgcat cccagagata aataaaagag aaactgcaac atcggtgtaa tcatgagtat
     6301 ctggctatag gggatactca gtaagcagct aataacaaaa gcgttgtagg caggtgcgaa
     6361 actcaccaat tgcaaatcct gctgatctaa ttgctcaagg cgtaacacgg gtttggcctg
     6421 agtgactttt agaataaaga tgaataaacc aataaataat accgtcccga tgacgccaac
     6481 ttcccacagc aacatactca gagaggtgga gtccagaatt aaattataaa taagattcaa
     6541 aaagcccgga gaaacagaac tgccgctgtt ggtagcattc agtccgtaac caaataacgt
     6601 accggaaata cctagcagat cattattctc ccaccagaaa aagagggtag taaagcgccc
     6661 tagctccccg gtcgacaata tgtaattcgg gtcaaaaata tagctcaacg attcccagaa
     6721 gacgcttaac gagctctgag tcggatcggt accaaaggca gccgaataga acgatgcgag
     6781 aataacaatc gccaatgtaa tcagcagcac catacccagg ataatcacca gtagcgcttt
     6841 caggtttacc ttactgatat ctttaacata gccgggcgtg acccacaccc atgccagaaa
     6901 cagaggtgaa agtaggatga cgaatttcac ttcgccaata atacacaacc ctaaaccaag
     6961 aataatatgc agaaccaaag acttaattgt cgttaaccca tgcttatatt cagaaagctt
     7021 tagcagcata attaacaggc aaaataagcc catcgctgcg gtatttccgc cgcccatcgg
     7081 atcaccgcca aaggtgccta cgaccgagtc ccacttttca tcttcaccga gcagggccac
     7141 acgctgaggc acgacaaaaa atacctgata gaacatcacc ggtatctggg cgtaaaacac
     7201 ccaatataaa caccgagtga cgcggtagat ttgcgactcg cggcaaaaac ccaacagcag
     7261 gcagatcatg accagcgaca ggcctatctc gtttttaaac gcgattatcg ccaccactgg
     7321 cccgccctgg atcaaggtcg aaaggccgac caacgccagg aaagaacaat agaccgcgag
     7381 aatgatggtt tcctgatcat ccaacccctg agtgccgtca cgcgtttgca tgaccagcag
     7441 gcccaccatc acaagggcga gacaaaatgg cagccagaga acggcctgca tgccggtaaa
     7501 atattgcact agcccgcaca atatcagggt tatgaacgtg taaatctgga gataaaatcc
     7561 ggtatttaag gtcatggcat caccgggttg ttcttcaaaa gcgtcgcacg tttattcatc
     7621 gccagataaa tgaatgcata acgaataaac gtcagaacgg agaaaataat caccgaggtg
     7681 gcatagtcgt tatagaaata aggcgtcact acaaaagcga tcagtacgat tgccagttgt
     7741 tccagctgaa tacgcaggaa atatcggtgt gatccaaaaa tcagttcgat gacggtgaga
     7801 ggactgaccg ccagcgccgg gaatagatag ggcagcatat aacgcgaggt atcaatggaa
     7861 ttgatccagt cctcaccgaa accaagatgc atcacaatag gataaaaaat aaacaccccc
     7921 agggtacata tcaccccgag caccatcagc aggaaacgaa cccgctggta ttccggatag
     7981 ttaaacgtgt tatttctgaa gtcgatcgac cactttgaga aaatggtatt acgcaccgca
     8041 ttcccgacaa tggtcaccgg tgacagacag aagcgactga ccacggaaaa atagcccgcc
     8101 gttaatgccg aaaaccaaaa gtttatgagc atgatcggca gattattgtt agccatcgcc
     8161 aatacttcgg cactcccgac tcgcgaaata tggtgaaaat gcgtcctgaa aaacaccagg
     8221 ttgcttttca gggacagatg ctgcgccgtg accgtacgga aattaaaaga tcgcgcgata
     8281 caactgatgg ttagcagcat catgccgctg gcccaggtcc agtaaaacat ctgaacctga
     8341 ttcgtcagta caacagacag caccacgacg actgacaccg aaatacgctg gaagatgaga
     8401 aaccggaaat tacccgtgcg cagtgacagg ttttccgata tcagaaccca tgcgttcgac
     8461 agcgataaca gatagagaaa cagcacgttt tgatggaata gccacgccgt cagtagcgcg
     8521 taaggcagcg ccagaatcgc gctttgcaga aggcaaaaca ccacattttg cgttaactct
     8581 tcttccgatt gtttaggaat aagcaattgg gacgcaaagg tacatacctg cgctccgatc
     8641 acagcgatac tgtaattgag cgcatacagg cccacttccg cgagatcgta tttatacgag
     8701 attaaccaga ttgagaaggc gccaatcagt tgcgaaatga ctgacgaccc ggcaatagtg
     8761 gaagcactct tcaataaact cataaccgac ctgtggtatt catcaattcg tcagtagtga
     8821 tattgagcga gcgaagactc tctttcgtct gcagattttt ttcctcaacc agattgagta
     8881 ccgctcccat gaccacttcc tggttggcgt caactttcgc cagcgccgcg cgcagcgtct
     8941 ccacggacgc agcccccgct tgcacaacga aaaccacacc atcaaccacc cgactaatca
     9001 gctcgatgtc ctgactctgg ctcaccgcag agacatcgac gatgatccgc tggtagcgat
     9061 ttcgcaggtc acgaatgaga ggttcaaaac gttccgaaga cagcatcagc aaagacgaag
     9121 cgttcgcttt tccgcggggt aaaaagtcca gcgtgtcact aagcgtgatc cgcgcagcgt
     9181 caagtgtcac ttctccacgc aacagctctg caactcccgc agaggtcgat gttgaaaact
     9241 cggcggacag gccatcacgg ttaaagaaat ccaaatcaag cagtaaggtt ttctgatcaa
     9301 agctgaagga gtttgccagc agactggcca gtaaagagcg cccctcaccc tgctttgcag
     9361 aggagattgc cagaacctgc tgcgggcggt tatccaacaa aatctgtgta cgaatgctgt
     9421 ggataatgtc ggcgttcaag gggtttcgcg tgatcaaatc gcgaacctgt gcacggtccc
     9481 cggcccccgc aatgcggcgg atctcaccca gtgagacgac gcccagtcgt tttgtcactt
     9541 gtccgagcgt cctgatggaa ttatccatcg ccgcttttac aatgacgtac ataaagaaga
     9601 aggccatggc cagcgccacc accatcacta acagtaacgc tttatttggc ttagatggct
     9661 tcactgccgg gacagccgga tcgtacagca ctgcatccgc attaatgccg gacagagaca
     9721 gttcctgggt ccgctgatac agggttttat aaagatcttc cagtttatcc agtgacaatt
     9781 tctggctgtt atacaggctg cgtttttcag ccagtttctg aaaaatttct ttctgttgat
     9841 caagttgcgc ctgataatcc ttttcatccg ccagcgcggc cagatattgc tgatgaatgc
     9901 cttttttaag ctcactgagc accacgccca tctgatcctg aatagccttc acctgagcct
     9961 gcgcttccag gatcttcgca tgtttcgggc catatgattt ttgtaactca tacaaagacc
    10021 gctgcgcctg aatcatggcg atacgcaaat cctgaatttg cgcatggtta gagatcgtcg
    10081 gtaatgaaat gatgttatcc agagagaccc ggcccccagc gctgacggct ttttccagag
    10141 attctgccgc aatacgccgt tgggtagcat cggccagacg gttggtaaca atgctgagtt
    10201 gctcggtttc gaagccatca atgccgcgga acgttaataa gccctctttc gccaggaagt
    10261 tatcgatatc ggctttctgc tgcgcgatct ctttctgcac ttcctccatt ttctgaaggt
    10321 ttacttctct ggctttttct gtcttcagcc gcttttggtc caacgtataa tcgataaacg
    10381 cctgtgcgac gccgttggca atctcagagg agagttgtgg cgatgtcgac tcataagaga
    10441 ctgtcgccag attagtggtt cgaataccgc taacggtaag atttttttgc agcgtgttca
    10501 gcgcgcgctc aatgcgctgc tgttcagcat cttcgctgtt gctggccttt tcatccgctt
    10561 ttttgccaat aaagtctggg ttttgatcca gctttaactc gcgaaccgct ttctccagaa
    10621 caatacgcga ctgcatcaag gcatattgcg tctcatagta gccggtgcgc gtggaatcaa
    10681 aatcttccac ctgtgggaac ggcgaaacgt tatcagcctg agcctttagc aacaccgtag
    10741 ctgttgagac atattttgat gacagcatgc tgattaatgg gtaggcaact gcaccgctga
    10801 taattcccgc aagtacaatc ttacaggcgt tctttttaat ttctttggca aatctggaaa
    10861 cgtcgacggt ctcttcccgt ttttttccat tttccactat tgataatttc atcgttagaa
    10921 aaatcccatt ccaatgatca ctacgtcccc aggacggaca gaatgggtca atgccacatt
    10981 ctcaagcagt tcattattcc ctgagagtcg gatattgata tctttgcgat ccgcgcgatc
    11041 ggtaaaccca cccgccaggg caatagactt ctcaacagtc atgccgggtt cataggcata
    11101 accattggga tttctaactt ctccagagac atagaattta cgaaattctg cgatgctgac
    11161 ggtaaccatt gggttttgca aataatgccc acgtagcttc ttggtaattt ctttccccac
    11221 ctgctctgcc gttttccctt tcagcatcag tttaccgata taagggaaag taatagcgcc
    11281 gcttttatcg atgataaatt tcatggtcat ctcgggttca ccgtaaacca tgatattgac
    11341 cgtatcgccg gcacccagaa tataactctc cgtgacagtc gacgcgtcgt ctgttaaagg
    11401 cggtcgggtg gatgagcaac ccaccaacaa aatactgcac agtaaaaatg tgcacaaatt
    11461 gattactttc attttatatc tgaaccttag cagtaagcat aatcattgaa ttgtcataac
    11521 ctaacgtcct tgttacttcc cgctcgtcgt taggtccaat gtagaacgaa tccgtattct
    11581 tattcgaatc caacttattc agaagatact tgagttcaac gttaaccgaa ggggtatagt
    11641 cgtaactcat tttcatcgta aacacgccat tcctgtcacg acgatttttg tcctgttttt
    11701 tgtaatcctc ccggctaaga gaataatcca gggtagtaga aaaacgatcc cccaaccaaa
    11761 agtgttggta cgaaacacca tatttggtga ataaaatata tccacccacc tcggacggat
    11821 ctttaatatt ctgtgaagta tgtagcgtaa caacagactg ttttagcggc ttccactctc
    11881 cctgtatgtc ccaattcaat ccattaaaat ttcgagcgtt ggcattattc tcaaacgtct
    11941 tatacaacca cgcgacattc atgtcgatgt ttgttttgtc cgataattgg gactttactc
    12001 catagcgcaa atagtactcg ttgctatctt tttcagataa acgatcgtat cgacgctggt
    12061 tagtaataaa actataacga aaacgtgtgt tcgcggtata ttggtcgtaa acttcagcaa
    12121 taagactatt ttcgtaccac tcctgctcaa ggagataatt gtaaaagtct acatcagcat
    12181 tacggacccc gcccgtctta ccgaaacgta actttttata ctgtaatgcc aactcagcct
    12241 tgccgcgtcc ttcaggtgca ccataactgt aacgcaattc gctattcaca aaatgggggc
    12301 tcaaaggaga cgtaataccg aactgtctaa attgttcctg gacaaaacct tctgtaattc
    12361 cgcgcccgcg ctcctcatgc cccagagaat cttccagatt cagggtcagt ccgtgtttca
    12421 gcccgtatcg ccatgcgccg ttaaaacgga aaaaatgatc gttgtagttg tcggcagagt
    12481 ccttatcgta acgacggtag tcaccggaat acatcagcaa atatttatcc tgaaaacgct
    12541 cgcctaccat acttaacgtc ggcgtaatgc tctgaaatac agaatctttc tccttgctgc
    12601 tgtgtggttg aaaggtgatg ttatctgaat aaccataatt cgctgcaacg tcgctttgaa
    12661 aatctacccc cgcaatgcct gtatgtgatt tcgggatggg ttcggcccat accggttgta
    12721 gaatg
//
LOCUS       DAPPNJ010000135        12580 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000135 DAPPNJ010000000
VERSION     DAPPNJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.136"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(74..2065)
                     /gene="tkt"
                     /locus_tag="RPM73_003150"
     CDS             complement(74..2065)
                     /gene="tkt"
                     /locus_tag="RPM73_003150"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDX6521485.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2343..3101
                     /gene="loiP"
                     /locus_tag="RPM73_003151"
                     /gene_synonym="yggG"
     CDS             2343..3101
                     /gene="loiP"
                     /locus_tag="RPM73_003151"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDX6521486.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3157..3900)
                     /locus_tag="RPM73_003152"
     CDS             complement(3157..3900)
                     /locus_tag="RPM73_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDX6521487.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3887..4996)
                     /gene="dgaE"
                     /locus_tag="RPM73_003153"
     CDS             complement(3887..4996)
                     /gene="dgaE"
                     /locus_tag="RPM73_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HDX6521488.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5000..5860)
                     /locus_tag="RPM73_003154"
     CDS             complement(5000..5860)
                     /locus_tag="RPM73_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDX6521489.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5857..6606)
                     /locus_tag="RPM73_003155"
     CDS             complement(5857..6606)
                     /locus_tag="RPM73_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDX6521490.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6632..7117)
                     /locus_tag="RPM73_003156"
     CDS             complement(6632..7117)
                     /locus_tag="RPM73_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDX6521491.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7128..7556)
                     /locus_tag="RPM73_003157"
     CDS             complement(7128..7556)
                     /locus_tag="RPM73_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX6521492.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7675..10479)
                     /gene="dagR"
                     /locus_tag="RPM73_003158"
     CDS             complement(7675..10479)
                     /gene="dagR"
                     /locus_tag="RPM73_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HDX6521493.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLFLKECRHYR
                     QRIDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRM
                     IQDRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDL
                     SQLAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDT
                     RMVILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVL
                     SLLTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNT
                     LSLKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEE
                     AYAIRLSDAEYFYIYELLYS"
     gene            complement(10738..11658)
                     /gene="speB"
                     /locus_tag="RPM73_003159"
     CDS             complement(10738..11658)
                     /gene="speB"
                     /locus_tag="RPM73_003159"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDX6521494.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11794..12525)
                     /locus_tag="RPM73_003160"
     CDS             complement(11794..12525)
                     /locus_tag="RPM73_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521495.1"
                     /translation="MKKWKVRSALIALVVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         3023 a         3520 c         3203 g         2834 t
ORIGIN      
        1 tgcttgtccg caaacggaca tatcaaggta ataaaaaagg tcgccgaagc gaccttttta
       61 cccgaaatgc taattacagc agttcttttg ctttcgcaac aacgttatca acagtgaagc
      121 cgaactcttc aaacagcagc tctgccggag cagattcacc gaaggtggtc ataccgacga
      181 tagcaccgtt caggccaaca tacttgtacc agtagtcagc aatacccgct tctacagcaa
      241 cgcgtgcagt aaccgctttc ggcagtacgg attcacggta agcagcatcc tgcttgtcaa
      301 atgcgtcggt agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt
      361 cgtaggcagc aacagccagt tcaacttctg aaccggtagc gatgaaaatc agttccggct
      421 gaccggcgca gtctttcagc acataaccac cgcgcgcgat gtttgccagt tgctcttcag
      481 ttcgttcctg ctgcgccagg ttctgacggg agaggatcag tgcggtcggg ccgtcctgac
      541 gctcaacacc gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag
      601 acatgttcgg ggttacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat
      661 cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt ttcatcagcg
      721 cagccatacg tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg
      781 gcaggaaacc accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa
      841 caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa ccagaccaca
      901 gggtcaggtt agacggtgcc aggtcagcag aaccgccgag gaattccggc aacagcggac
      961 cgaaagcttc gatagcattc tgagacgctt tacggctggc gattttcgcc gggttagcct
     1021 gcagtttagc aatgaactct tttgctttcg cgtcgaagtc agacggcatt tcgcctttca
     1081 tacggcgggt aaattcagcg gcttcctgcg gataagcttt cgcgtaagca gcgaatttct
     1141 cgttccatgc ggattctttc gcctggcctg cttctttcgc atcccactga gcatagattt
     1201 cagacgggat ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt
     1261 cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga
     1321 aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt
     1381 ctactgcgcg tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc
     1441 cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg tgaccatcaa
     1501 tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca
     1561 gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcatagg
     1621 tgtagtggtc gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg
     1681 caatcgccat accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca
     1741 caccagcggt gtaacccact tccgggtgac ccggagtttt agagtgcagc tgacggaagt
     1801 ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca
     1861 tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg
     1921 ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg
     1981 cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat
     2041 tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct
     2101 taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc
     2161 aagccgcgcg tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc
     2221 tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact gagactgagc
     2281 gtcgattcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa
     2341 acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg actggttgcc
     2401 agaatatgga ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt
     2461 tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg
     2521 cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc
     2581 taggcaacaa tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga
     2641 acgcctttgc aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga
     2701 cggataacga agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg
     2761 tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg
     2821 cgggcgggat tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag
     2881 tcaattcgca attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc
     2941 tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcaaaac
     3001 tggaagaagg tcgccaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgcgccc
     3061 agcatattcg cgatcgtatg agcgcggatg gggttaagta agtcagacat accgatctac
     3121 aacataattg taggtcggta acgcctgaat ccccgatcag agtagtgatt tcactttatt
     3181 tagcaaaacg gctacatcat ccggtcgggt atatcccgta tcagggtcta taatcgagct
     3241 gtaaacatgt ggcataatgc gcggcacccc cgcctccaga caagttttca gaatcacact
     3301 gaagttatcc aaatcaatcc cgccagtcgg ctcaatcagc gtcataccat tgcgcgccgc
     3361 cgtggtcgcc agcgcgtata gctccggaag cgacctctcg ccgcccatcg gaaagaactt
     3421 cgccgcgtgt gcgcccatat ctagcatcat tcgcaccgcc gtatcgcagg agacgcgggc
     3481 aggcgtaccc tggctgctgc taacgccggt ggaaatcagc acttcgcccg gcgtccccgt
     3541 cgggctaacc agcgcgttga catgggtctg ctcaccgccc gtagccgcaa gcgcgcctgc
     3601 cgcgaaacca caaccggtaa aggtctggtt aacgtgtgct ggatgaatct gcgccgcaat
     3661 catcgcagct ttgtaatact gcgccgggtc acccgccccc agacctactg aaatggacgg
     3721 tatttccgcc atccaacgtt taacttcctg gatcccctca tccaccgagg agaactgcgc
     3781 ggaaagcacg ccaatcactg cgtggccttc ggcggcgtca aagatttcac gggcattggc
     3841 gatatctttc gccagcacat taattgccac gcgattacga tagaaattaa tcttttgcat
     3901 gatctgcaat ctccttcagt cgcgccacga taagtgccat ttccccttcc gccaccgtac
     3961 gcggatcaag gctaaaaacg ccctggtgga ggttataccg ccgtgcataa atcgcgatat
     4021 cgccaccacg cagttgcgct tcgacgacgc gagcgtcaac tcccaattcc tgcgcattta
     4081 ctcgaatacg aatacgccag attgcacgcc cggcttcatc ctgctcaata tcagccgtta
     4141 acccgtgaat cgcagaaatc gcttcaacca ctggctggag ctgttccgcg gtgatgactg
     4201 cctgcccctg gtgataattc tccagcgcgt aaaccagccc aaccatgttc tccttgccga
     4261 ttttcatcgc cctggcaatg ccgtgatgct gcgctttgca ggctgcaatc cagatttttt
     4321 taccagtgat aaacccggag gtcggcgcgt taaaggcttt tgcgccgctg tagaccacca
     4381 tatccgctcc gctcaccacc caggcacgta aatcctcttc ggcggcggca tcgacaatca
     4441 gcggcaggtt gtacatctgc gcgacatgtg cgaagtcctc gatgttcaac attcccttct
     4501 gcacgcagtg atgcgacttc acatacagca gcgcggcggt cgcgtcggta atggcactct
     4561 ccagctgcca gagagccgcc aggttacttg aacccacctc cacaacgcgc ccgccaccta
     4621 agcggatcgc gctgaggatc gatgcaccgt aatcgacgtt atggccgcgc agcatcacca
     4681 cttcattggc catgccggag ctatccggca tgagcgccac ccgggccttg tcgccgcgtg
     4741 taatggcggc ggcgacagcg atggcgatcc ctgctgaggc acaggaggta acgtagctat
     4801 cctccgcccc ggtaaagcga gagaccagct cgccggtttt gtccaccagc tggtcaatct
     4861 ccacgaaaga ggatgccgcg cgcgcggtgg cctgcatcac ctccggcgcg acgctggaga
     4921 cgccgagaat cgtcatcttg ccgcaggcgt taatcacctg tttcagtccc agttgttgat
     4981 agatattctg cgtcatggct tacagcactc ccagcagcga gcagaccacg ctcaacgcca
     5041 caatcgacag cagaatcgtg gtgtaacgcg gacccttttt caccaaatag aagtagatgg
     5101 cgaacactgc cgccagcggc agcaggccgg gcgcgatgga gtccagaatc tgctgtacca
     5161 ccacttcaga gcctttcagt gcgctaattt ttaaaggtga ggttatcttg acaaagctcg
     5221 cggatagcgc gcccatcatg ataagcccca gtacgttagc gccgtagata agctccttga
     5281 tgcgccctcc ctgcagcaga ctgataatcg aatcgcgccc cagcgtatag cccttgtgca
     5341 ccatgccgta gctgattgcc agcgtaatcg ccgggtagag aatcagcggc aaaataccgc
     5401 ccatcgcgct gccattggcg gcgaacggga taaaaatagc aatcaacagc ggcatcaccg
     5461 ccgcccagat gatggagtcg cccatcccgg ccagcggccc catcaggccg gttttaatcc
     5521 cggtgataga ggcatcgtta atcgcctcgc cgcgggtctt ttgctcttcc atggcgatgg
     5581 agatcccctg gatcaccgcg ccaaacgtct gctctgagtt gaagaagttc agatggcgtt
     5641 ttaacgcctc cacctgctcc tccttttgcg gatagagctt tttgataatc ggcgtcatcg
     5701 aggcgcagaa aatcaggctc tgtaaacgct cataggagct ggaaacttcc gcgcccagcc
     5761 agtagataaa ccacgctttg gtgatatccg ctttggtcag cgccccggac tcccgcgcgc
     5821 gctcaaccag ttcatgctgc attacgtcgt cgctcattat gccgctcctt catttttcgc
     5881 caggccttta atcaggaagg ccacgcaggt gccgaaaatc gccatcgcca tgatgtccac
     5941 cttgaggtac agcaccgcga agaatccgcc gataaaccac ggcagcaggc tctttttacc
     6001 aatcaccata atggtgatgg cgaagcccag cgccgggaga atcccgccca tgatttcaaa
     6061 cgagtgggtc agccagtgcg gcatcagctt gaggaaactt tccactacgt tctggccgaa
     6121 gtagttggcg gcgaacacca ctgggaaacg tagtaccagc cccagcagcg ccgggtagag
     6181 aaaggcgcag cgcataatgg ctgacatatt ggccgtttcc gcgtgtttat cggccatatg
     6241 cacccaggcc gcatttagcg tgcgacgcaa ctggtcgagg aatacgccga taacgccaaa
     6301 tggaatcgcc agtgcaatcg ccaggttcgg gtccatattg gccttaacgg cgataggaat
     6361 ggcgatacag gccgccagcg ccgggtctga cggcacattg ccgcctggcg ttgaggtcac
     6421 acccagatag accaactgca tccccgcgcc gataatcatc gccgcaggca tattgccgag
     6481 cagcaggccg acaaagaccg ccaccaccac tggctgtaac agcatggcgg agaaggtata
     6541 gccaaggcgc aaccgggcaa accagtaata cagccccatc aggcttgcaa aaagtaaggt
     6601 atccataatt tgttatccca tgttcagagg gtcagaattt tttcaggata tcgtccagcg
     6661 actgcggttt atcttccggg atggtctgga aaacagcctt aatgccgcga cttttcaaat
     6721 cgttaagaat gccaacgtct ttttcatcca gcgttatgtt ctggaacacc gctttacggt
     6781 ttggcccgcc gcctaaaccg ccaacctgaa tatcagccac gtcaaagccc tgctgcaccg
     6841 cttcctgaat cgccgccagc gacgggaaca gcaccagcac gttgccgtcg cccagctggt
     6901 tctctttcca cgcggccgcg aagctctggt tgctaaagca gtccactttg atattcggcg
     6961 gcgcggccat caggtagata tttttcataa acggatcggc gtccagctcg tcgctgacca
     7021 ccgcaatacg gttggcctga gactgcccta cccacttcgt caccacctgt ccgtggataa
     7081 gacggctgtc aatgcgacat aacacgatgt ttgccatacg attttcctta ctgttgtaat
     7141 tgacgaatat gggagacgac gtccaggcaa ctgttgcgcc cagcttccac cagatcggcc
     7201 acgcaggcgg tcagtgggcc atgttcgcgc ttgtccagcg cctccagcag cagcgaggca
     7261 ttaagcccgg agatcaccgc caccggataa tcggcgctca gccgcgccgc cacgttagag
     7321 gtggtgccgc cgataaaatc ggtcacgata agcgaacctt ccggcatggt tttcaccacg
     7381 gcttcgacgc gctggtaaaa ctcgcccagc gtatcgacgg gcatcagcgc gatttccgtc
     7441 acgccttcga tttcgcccat caccatgcgc agactgttgc agagctgcag cccccagccg
     7501 ccgtgagtta gcagcagaat ttcaggtaac ggatgaatag gtacagttgt ggtcaaaata
     7561 gcctcctttg ctccagtgtt tacatttcat caggagcaaa ggacgtgcca gaatttgtgt
     7621 cacgcacaga ggaaatgaga ttggatgggg cgttaagccc cgtaaaccag gggatcagct
     7681 gtagagcagt tcgtagatat aaaaatattc tgcgtccgat aaacggatag cgtaagcctc
     7741 ttcaatcggc aaaaaggagg atttgatgac gctaaaggca cgagcgtcca gatcaagccg
     7801 gttttctagc gccatctgta acggtttgcg gttaatcacg atacgctcga ccatgcagca
     7861 gcagtggatc agaaaacgta gtgtcacctg gcggctcggt ttcagggaga gcgtgttggt
     7921 taagtagttc agaacatcct cgatctcttt cagaattcgc tgcgggttga gcaccgaaat
     7981 atggttgatg atgctctcca tggtcagcgc gctgataaag cgcatggcgc tgcgctccat
     8041 ctccagtcgg cgctcgctgc ttgagagatc cggcgtcagc aggctgagca ccagctccgg
     8101 cccttgctcg gaaaacagtt cctccagcga gataaacggt atatccggca gtcccggctg
     8161 gaaggtgccg acgatccctg ccagccgttc actggcatta agcgcctgct gaatacgctc
     8221 caggctgcgc acctcgttat agtcgagaat caccatacgc gtgtcctgcg acatcagctc
     8281 gccaaagctc tcctccagca cctttttgat cttttccgcc gtccccattc cggttataca
     8341 tgagataacc agcactttgc caccgctctc ttgctgcggc gtgcagagcc gacaagggat
     8401 atttttactc tgcataagca cagccaattg cgatagatcg ccagtttcgt agcttaaatc
     8461 cagcccaacc tccagcaggc tggtcagagt gatatttggc aacaacagaa cgtcaatctg
     8521 gaacagcttg ctgatggtgc tgccaaaatg gaccagcgat ccgatatcca ccatcagtat
     8581 cagccgctgc cagcaccggt cctgaatcat ccgggtcagg gtttccaggg tgtcatgcac
     8641 cgactgctca aatggcatat caattgcgta gaacagatcg cgctccagca cccggttcac
     8701 atattgcgcc tggctcgttg cggtggtcgc accgtgagcg attagaatca cgccgcaatc
     8761 cgggctggtg tcgatgcgct ggcggtagtg gcgacactct ttcaggaaca ggaacaggca
     8821 cagccagacc acctccgtcg ccgggcactg aatatgcaac aactcattga ttttgcggca
     8881 cagaagcgtg gcattgtcat actcatcttt acagcgatcc agaatcaggc tggatgaata
     8941 gagttgaggt ataagccctc gccgcacata gccaatcagc gcaagaaagt gtttacgtaa
     9001 cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc tgctccacgt aaccgatcag
     9061 cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt ggatgcgccg cactatggcg
     9121 gctataaaga ccatattcaa aaatcgaact gagtttgttt ttcaggatag ccagcgtttc
     9181 tgcgggcggc acgttactgt tgcgcagatt gacatattca cgagtcagga aactgtaaaa
     9241 caggtcgctc tcttctattt ccgcgctggt cgccaacgaa ttttttaacg cgggcaacgt
     9301 acgcgcatcc acgttcagac gatctttacc gccaaacaac gcatcgacca gcagacgctg
     9361 ctctggcgtg gtattaaacg acatctccgc cagtcgttta tctaactcta gcgtatcatt
     9421 acgctcggtc attcccgacg cccacgcctg cgcgcacaag aactgaatat cacttttaag
     9481 ctggccgata ttgccttcca ggggtttact taacaaccaa tgcaagagcg ttttatcaag
     9541 gcttatggta cgttcaattt tacgactttc acgctgaaag aagcttacaa tcaattcgag
     9601 ctgctcctcg accgaacgct ggcgaatacc cggtaagtca atacatacct ggatacgccg
     9661 ctggaaggta cgcaatagtg ccgagttgac cgactctgta gtggcgcaaa taaggcgcac
     9721 cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg tattcacctt tatcgagaat
     9781 agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc acttcatcca gaagtaaata
     9841 acctccgtca gcttgctcca ccaatcccgt tttattctca cttgccccgg taaatgcccc
     9901 cttatggtga ccaaacagat gtgaggaaag caactccgga ttatgagcat attcggcaca
     9961 gttaaagtaa accaacggag gtggcgaacc tatggtgtgc ttgcaagcga agcaatgcat
    10021 taactcagca aagaatgttt tgccaacacc agatgcaccg gttaacagca catgtagtcc
    10081 gtggggataa agcaccgctg cgcggccttt ttctaccgca tcacggagac tacggtcgta
    10141 tccgataagt ccggaaaacg gatcatcatc tgtatatttt tctttctttg gcaataaact
    10201 agccactgaa tgaactatgc attcgcaatc atccagtttt cgattaatga gcaattctag
    10261 tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt tttaccacca ggccttcatt
    10321 ccataattga ttaagatctt tactaaccga gttgcgtgcc agcccaaggt taaaaccaat
    10381 cgcctcagca gtaaatgctt tctccttcgc taaatcagac agttccagcc catacgtcag
    10441 ccgctccagc tccttgtgaa taacatcaat ccgtctcatt tctttaccgc ttaaaaaaca
    10501 atagttgttc agaacataca ctatttgtaa acgtaagaat gagaactatc gggaagaatt
    10561 aatgttcctt gtggcaatct ggtttctgaa tattcgtgtt ttaaatatta cttatggtct
    10621 gaaacaacca ggcaggtgaa cagagctaag aaaaagtagc gtagaaatat aaaaacatat
    10681 atcgcactga tgaatgtttc ccggcatgac tatgcacacc gggaaatatt tagtaaatta
    10741 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
    10801 agcggtgatt tccgactgat catatgccgg agccacttcc actacgtcca tcccaacgat
    10861 gttgagatct ttcaggccgc gtaccagttt aatagcgcga tcggaggtca ggccgccaat
    10921 cactggcgta ccggtgcctg gtgcaaaagc aggatccagg cagtcgatat caaaggtcag
    10981 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctgcg
    11041 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctatatcaa actcggtacg
    11101 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
    11161 catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg
    11221 taccagcgcc attttaccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
    11281 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
    11341 ctgcagcttc tcgctcatct cacgggcatc gccgaaagca tataccagat caccgcagtc
    11401 cacgacgttc agacgctcgc gcatgtcgaa attccacggg aagcggttgt gttcccaggc
    11461 cagattcgtc gaaacctggc gaatcgccgc cgggccgtga cgaccaccgg cacggccaga
    11521 ggtggccatg tcgaacggca cgccggtgat cacccagtcg gcatcgctgt catacggctg
    11581 gaagttcatc ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg
    11641 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
    11701 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
    11761 cgccctaaat gggcgaggca tcaagcgagg ttgctatttc cgacatggtg gcggcgcata
    11821 gccattgttg tccgccggaa acgcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
    11881 gccttgtaat tcaaaggcgg caaagccgat tacaccggta ttacgcacat cgccttgcgc
    11941 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cggtatgttt
    12001 atctttgcgg aatcctttaa tcgctaacga atctccggca ttcacaatat aaccgttgtt
    12061 attcagcgaa ccttgtttac cattcagaac atccaggcca tcaaccgtag caacgatctc
    12121 gtagttggtg ttacgactgt aattgcggac atataattga taattcattc cgactctggc
    12181 atggacaata tagcgtggtt cattattgga tgaatcataa gcccgcgtaa taggtagaga
    12241 attaaaatta ccatcgcgaa tagcatattc cagttcgtta atacgaatgc tataaacgcg
    12301 gtcgtagccg gaaggaacat tagtcgaata actaataacg atcatctctg caggatcgcg
    12361 ccaggctcgt tcagcagata cgccctgaac cgatgagtgc acatccccac cccaggcggt
    12421 gccgacattg cccgatgcac tgctattata ctgcgcatta ctggaacaac cggccagcag
    12481 cacgactaat gctatcaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc
    12541 atttaattaa tgcagcgatt gtagggagat tatttaataa
//
LOCUS       DAPPNJ010000136        12517 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000136 DAPPNJ010000000
VERSION     DAPPNJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.137"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            64..1578
                     /gene="caiT"
                     /locus_tag="RPM73_003161"
     CDS             64..1578
                     /gene="caiT"
                     /locus_tag="RPM73_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDX6521496.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1609..2751
                     /gene="caiA"
                     /locus_tag="RPM73_003162"
     CDS             1609..2751
                     /gene="caiA"
                     /locus_tag="RPM73_003162"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDX6521497.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2880..4097
                     /gene="caiB"
                     /locus_tag="RPM73_003163"
     CDS             2880..4097
                     /gene="caiB"
                     /locus_tag="RPM73_003163"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDX6521498.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4171..5724
                     /gene="caiC"
                     /locus_tag="RPM73_003164"
     CDS             4171..5724
                     /gene="caiC"
                     /locus_tag="RPM73_003164"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDX6521499.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNQYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5833..6618
                     /gene="caiD"
                     /locus_tag="RPM73_003165"
     CDS             5833..6618
                     /gene="caiD"
                     /locus_tag="RPM73_003165"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDX6521500.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6624..7214
                     /gene="caiE"
                     /locus_tag="RPM73_003166"
     CDS             6624..7214
                     /gene="caiE"
                     /locus_tag="RPM73_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDX6521501.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7300..7695)
                     /gene="caiF"
                     /locus_tag="RPM73_003167"
     CDS             complement(7300..7695)
                     /gene="caiF"
                     /locus_tag="RPM73_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDX6521502.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVI
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7956..11177)
                     /gene="carB"
                     /locus_tag="RPM73_003168"
     CDS             complement(7956..11177)
                     /gene="carB"
                     /locus_tag="RPM73_003168"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDX6521503.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11195..12343)
                     /gene="carA"
                     /locus_tag="RPM73_003169"
     CDS             complement(11195..12343)
                     /gene="carA"
                     /locus_tag="RPM73_003169"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDX6521504.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2906 a         3265 c         3331 g         3015 t
ORIGIN      
        1 ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt cgttttcaca gtggaattaa
       61 ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccgtta
      121 ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcagc gaatgtcgtt
      181 attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg
      241 gtggtgatgc ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta
      301 ggtaacgaac cacctgaatt tagtaccgcc agttggatct ttatgatgtt cgcctcctgt
      361 acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg
      421 ccgtttggct tagaaccgaa ctcgacaggg gcgaaagagt tggggctggc ttacagcttg
      481 ttccactggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac
      541 ttcttctttg tccgcaaaat ggaagtgatt cgccccagct cgacactggt gccgctggta
      601 ggtgaaaaac acgccaaagg gttgttcggc actatcgtcg acaacttcta tctcgtcgcc
      661 ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg
      721 caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg
      781 attatcctca acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt
      841 gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcattgt cagcggtgcc
      901 agcttcatca tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcgc
      961 atgttgttct ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc
     1021 tactgggcat ggtgggtgat ttatgctatc cagatgagta tcttcctcgc ccgcatctcc
     1081 cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtgc tggggctgac agcgtcaacc
     1141 tggatcctgt ggactgtact cggtagtaac actctgctgt tgatagataa aaacattatc
     1201 aacattccaa atctgatcga acagtacggt gtggcgcgcg ccattatcga aacctgggcc
     1261 gctctgccac tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc
     1321 gttacgctgg ttaacgcctg ctcttatacc cttgcgatgt ccacttgccg cgaagtacgc
     1381 gatggtgaag aaccaccgct gctggtgcgt atcggttggt caattctggt tggcattatc
     1441 ggtattgttt tgctggcgct cggcggcctg aaaccgattc aaactgccat tatcgccgga
     1501 ggatgcccgc tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa
     1561 cagaactgga aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat
     1621 ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac
     1681 tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa
     1741 gcactggcgg atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac
     1801 gcggggtttg ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc
     1861 tatgtactgt accagttgcc gggcgggttc aacaccttcc tgcgcgaagg cacacaagag
     1921 cagatcgaca agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt
     1981 accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat
     2041 ggtaagattt atcttaatgg tagtaagtgt tttattacca gcagcgccta taccccgtac
     2101 atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt
     2161 gttgatatga gcaaaccggg catcaaagtg accaaacttg aaaagctcgg tctgcgtatg
     2221 gatagctgct gtgaaatcac ctttgacgac gtggaactgg acgagaaaga catgttcggt
     2281 cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta
     2341 gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac
     2401 cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc
     2461 cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca
     2521 gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg
     2581 gcatttgaag tggtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac
     2641 caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtctccgg gggatctgac
     2701 gaaatgcaga tcctgacgct gggtcgcgcg gtgctgaagc aataccgcta agtccgtgcg
     2761 ctgcctgatg cgacgctaat gcgtcttatc aggcctacgg gagcacgaat gtaggccgga
     2821 taaagcgttt acgccgcatc cggcagtcat gcgctacata aatttttcag gagctaataa
     2881 tggatcatct acccatgccg aaattcgggc cgctggccgg attgcgcgtt gtcttctccg
     2941 gtatcgaaat cgccggaccg tttgccgggc aaatgttcgc agaatggggg gcggaagtta
     3001 tttggatcga gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcagc
     3061 tctcccgccg caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag
     3121 cgtttctgaa attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct
     3181 ttgcccgtcg tggcattacc gatgaagtac tgtggcagca caacccgaaa ctggttatcg
     3241 ctcacctgtc cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata
     3301 acaccatcgc ccaggccttc agtggttacc tgattcagaa cggtgatgtt gaccagccaa
     3361 tgcctgcctt cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc
     3421 tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt
     3481 acgaagtgat gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa
     3541 tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc cggttgcggt ctgtataaat
     3601 gtgccgacgg ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta
     3661 aagatattgg cctcgcacat ctactcagta cgccggaaat cccggaaggc actcagctta
     3721 tccaccgtat cgaatgccct tacggcccac tggtagaaga aaaactcgat gcctggctgg
     3781 cggcacatac catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca
     3841 aagtactgac cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca
     3901 ctcagtggca aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca
     3961 aaaataaccc cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca
     4021 ttttgaaaaa tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg
     4081 ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat
     4141 aacaaacgaa caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
     4201 caaatgtggg acgatcttgc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
     4261 agcggcggag tcgttaacca gtatagttat cttgagttaa atcaggagat taaccgcacg
     4321 gcaaacctgt tttatacgct ggggattcgc aaaggcgaca aggttgcact acatctcgac
     4381 aactgcccgg aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg
     4441 ccgattaacg cccgcctgtt acgcgaggaa agcgcgtgga tcctgcaaaa tagccaggcg
     4501 tgcctgctgg tgaccagtgc gcaattctat cctatgtatc aacagattca gcaggaagat
     4561 gccactcaat tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggcgtg
     4621 agttcgttta ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
     4681 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt
     4741 gtggtgatta cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
     4801 ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt
     4861 actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc
     4921 gcccgcgcct tctggggaca ggtgcagaag taccgcgcca ccattaccga atgtattccg
     4981 atgatgatcc gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg
     5041 cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagatgcgtt ttgtgaacgc
     5101 ttcggcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
     5161 gatcgccctg gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa
     5221 gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt
     5281 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
     5341 gcgaaagtgc tggaagccga cggctggcta cataccggcg ataccggata ctgcgacgaa
     5401 gagggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
     5461 gtctcctgcg tggagctgga aaatatcatc gccacccatc caaaaattca ggatatcgtg
     5521 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
     5581 gaaggtgaaa cattgagcga agaggaattt ttccgcttct gcgaacaaaa tatggcgaaa
     5641 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
     5701 ataattagaa agaatctgaa ataaatgaaa cagcagggaa ctaccctgcc agctaataat
     5761 cacgctataa aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa
     5821 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
     5881 acccttgatc gtccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
     5941 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
     6001 gagaagttct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
     6061 gctgactttg gcccgggtgg atttgcggga ttaaccgaaa ttttcaatct cgacaaaccg
     6121 gttatcgcag ctgtgaacgg ctatgccttt ggcggcggtt tcgaactggc gctggcggca
     6181 gattttattg tttgtgccga taacgccagc ttcgccctgc cggaagccaa gctgggcatc
     6241 gttcctgaca gcggcggcgt gctgcgtcta ccgaagatcc tgccgcctgc catcgtcaat
     6301 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc
     6361 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc gcagcagctg
     6421 gttaacagcg ccccgctggc gattgcggcg ctgaaagaga tcttccgcac caccagcgaa
     6481 atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccgtcg
     6541 gttctgcatt cggaggatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg
     6601 gtgtggaaag gacgttaaca accgtgagct attacgcctt tgaggggtta attccggtgg
     6661 ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag
     6721 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
     6781 aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca
     6841 tcgttgggga aaacggccat atcgggcacg gagcgatcct gcatggttgt gtgattggtc
     6901 gcgatgcatt ggtcgggatg aacagcgtga ttatggatgg cgcggtcatt ggcgaagaga
     6961 gcattgttgc cgccatgagc tttgtcaaag caggctttca cggcgagaaa cgccagttgt
     7021 tgatgggtac gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac
     7081 ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa
     7141 cacagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
     7201 cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg gataagatgc tacaacatcg
     7261 catctggcaa gcaggacgcc cggtatcaac gagataaaat taacgacgca tactctttga
     7321 caacatcatc tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg
     7381 cggcgtcttt attacgccga ctaatttatc ccggctgtta ttgcgcaggc gcgcgtaaat
     7441 ttgctcatcg atatcgacca cttttaccag tcgctggcac tggcatcccc gtccttccag
     7501 cttattaggg atcatcttaa cttcgcagct tatttctgcg acttccgaca gaatataagt
     7561 cagggtatta accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat
     7621 cacccaccga ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac
     7681 atatccttca cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt
     7741 tgaagcaatt aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac
     7801 cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga
     7861 tttacttaat gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa
     7921 attagcggat gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc
     7981 tgcaccgaaa ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca
     8041 ccgttcaggg tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg
     8101 gagtcttcaa tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc
     8161 ttgatgcggt cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta
     8221 atgcccgctt cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt
     8281 ttcagcagtt ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc
     8341 aacgcacgac cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct
     8401 tcagcgaagg tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac
     8461 agcgggtcaa cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac
     8521 ggcgggataa cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc
     8581 gccgccactt ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc
     8641 gcacgcgggt taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc
     8701 atcagaccgc gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc
     8761 tgaatttcct gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg
     8821 cccgcctgct cgatgtgctc catgatgccg ccaataagca ccatttcgcc gtcgcagatg
     8881 gcatccacgt caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg
     8941 ttagacacgc tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt
     9001 tccatcgccc gaccgccgag aacgtaagac ggacgtacca ccagcgggta gccaatctct
     9061 ttcgccttct ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt
     9121 ttcagacgct caaccgcatg ctgaaagcgt tcacggtctt ctgcacggtc gatagcatcc
     9181 gggctggtgc cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg
     9241 gtctgaccgc cgtactgaac gataacgcct ttcggcttct caatacgcac gatttctagc
     9301 acatcttcca gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag
     9361 acggtttccg ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagcgccagc
     9421 gaggcgtgta cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg
     9481 ccgagaacca tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa
     9541 gtggagtaca tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta
     9601 taaaccgggt gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc
     9661 gccagttttg ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca
     9721 gcgttcaggc cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca
     9781 atctgtacca ggaaccaacg gtcaatattg gtcaggttga acacgccgtc cacagacagg
     9841 cccgcacgga aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg
     9901 cgacggattt tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc
     9961 gcaccgactt ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc
    10021 gccatcactt cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat
    10081 ttttcgaagt tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag
    10141 gccggagtac gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc
    10201 agtttcgccg ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa
    10261 cgggacacgc gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca
    10321 aactggacat tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg
    10381 ttacgcatga tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag
    10441 tcaccggtat ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg
    10501 tcgtttttat cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca
    10561 atcagcagct ctttggttgg ggagagatcc agaccgcggg cgcaaatttc ttcaaactct
    10621 tcgcggttat aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg
    10681 cacgggaagc ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg
    10741 gaacgcgcgg tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct
    10801 gctttatcaa tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg
    10861 ccctgacgct ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc
    10921 accgcgtccg ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggctcg
    10981 atgtaggtcg catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc
    11041 agaatgaccc ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca
    11101 aactcacacg cctgaccgat aacaatcggg cccgccccca gaatcaggat actttttata
    11161 tctgtacgtt ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct
    11221 caattaactc gataaagtgg tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt
    11281 cagggtgccc ctggaagctg aacgccggtt tatcggtgcg atgaatgccc tgtaacgtac
    11341 cgtcgaacag ggatttatgc gtgacacgca ggtttgctgg taatgttgct tcgtccaccg
    11401 caaaaccgtg gttctgggcg gtgatcatca ccacgttttt ctcaacatct ttaaccggat
    11461 ggttgccgcc gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca
    11521 gcagctgatg accgaggcag atgccgaaga ccggaatatc ggtttcgagg aatttctgga
    11581 tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc
    11641 cgtctggatt cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc
    11701 gacagcctct gtccaccaac atgcgcagga tgttgcgctt ggcaccaaaa tcataagcca
    11761 cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc
    11821 agctcccttg tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc
    11881 cattcagacc tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc
    11941 ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg
    12001 tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg
    12061 tattacggaa gttgctggca atcagcggca ggtcgcgaat caccagacct tgtgcatgta
    12121 cctgagaaga ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa
    12181 gagtaacgat ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg
    12241 aagtattgaa aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa
    12301 actgggttcc gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat
    12361 attcactcac tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc
    12421 ttaccgacga atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact
    12481 taatggcggg attttttata ttattttgtg gctcatg
//
LOCUS       DAPPNJ010000137        12293 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000137 DAPPNJ010000000
VERSION     DAPPNJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.138"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(27..179)
                     /locus_tag="RPM73_003170"
     CDS             complement(27..179)
                     /locus_tag="RPM73_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDX6521505.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(444..1178)
                     /gene="cysH"
                     /locus_tag="RPM73_003171"
     CDS             complement(444..1178)
                     /gene="cysH"
                     /locus_tag="RPM73_003171"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDX6521506.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1252..2964)
                     /gene="cysI"
                     /locus_tag="RPM73_003172"
     CDS             complement(1252..2964)
                     /gene="cysI"
                     /locus_tag="RPM73_003172"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDX6521507.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2964..4763)
                     /gene="cysJ"
                     /locus_tag="RPM73_003173"
     CDS             complement(2964..4763)
                     /gene="cysJ"
                     /locus_tag="RPM73_003173"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDX6521508.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5082..5444
                     /gene="queD"
                     /locus_tag="RPM73_003174"
     CDS             5082..5444
                     /gene="queD"
                     /locus_tag="RPM73_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDX6521509.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5522..6793
                     /locus_tag="RPM73_003175"
     CDS             5522..6793
                     /locus_tag="RPM73_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX6521510.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6784..7044
                     /locus_tag="RPM73_003176"
     CDS             6784..7044
                     /locus_tag="RPM73_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDX6521511.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7061..7636
                     /locus_tag="RPM73_003177"
     CDS             7061..7636
                     /locus_tag="RPM73_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDX6521512.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7784..8644)
                     /locus_tag="RPM73_003178"
     CDS             complement(7784..8644)
                     /locus_tag="RPM73_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDX6521513.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8641..9420)
                     /locus_tag="RPM73_003179"
     CDS             complement(8641..9420)
                     /locus_tag="RPM73_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDX6521514.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9398..10807)
                     /locus_tag="RPM73_003180"
     CDS             complement(9398..10807)
                     /locus_tag="RPM73_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6521515.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10829..12283)
                     /locus_tag="RPM73_003181"
     CDS             complement(10829..12283)
                     /locus_tag="RPM73_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDX6521516.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2797 a         3394 c         3158 g         2944 t
ORIGIN      
        1 cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag aactgcctta
       61 aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac catcagcgtg
      121 aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt tgtcagcatg
      181 tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga acgtaagcct
      241 caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg aatgcttaac
      301 gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc agcggcgttc
      361 acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag
      421 gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct ttttaagcca
      481 aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt atgggtatca
      541 ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg tttttgcagg
      601 tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa tacgccacgc
      661 tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg caggccagca
      721 aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac tttgttgatg
      781 tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta gcgtgcttcc
      841 tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag tttgtccgtt
      901 aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt gaggatcacc
      961 ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg aatgccaaag
     1021 ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac gcggccttca
     1081 gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg atctaccttc
     1141 ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact gtttgccttg
     1201 cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg gttaatccca
     1261 caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc
     1321 gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat caagcgacgc
     1381 caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg tcccaatgcg
     1441 gttgccgcca agatgcagat tgtagcgacc cggtgcttta cccaccaggc ccacttccgc
     1501 cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga tatgctcatc
     1561 gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg
     1621 ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca tcgagttttc
     1681 acgctgcggc gtgacggcat tcattaaacc gctctctttg gcgatcttct cgatcttcgc
     1741 tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc
     1801 gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc ccggataatc
     1861 aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa tgcccttaac
     1921 ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt caaatttgat
     1981 ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct ccagcgtgta
     2041 tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag ttgtcacgac
     2101 ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc tcgccgtgcg
     2161 ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca gcaggttgaa
     2221 gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg catgcagatc
     2281 gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca ggtaggtctg
     2341 accaaggatc ggttcttcat cagtagtggc gactttttcc tggtcgagcc agatctccgc
     2401 atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact cgtacgcttc
     2461 cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt tcatgtcgtt
     2521 agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg gtttgacgtt
     2581 ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa tgctgccata
     2641 gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg taatcacccc
     2701 acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct gttcagcacg
     2761 ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga tcagcaggaa
     2821 gttgtcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg
     2881 caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa
     2941 aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg ctctacgcgc
     3001 agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc ggcaatcact
     3061 tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca gacataaatg
     3121 tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag tttgtcttgt
     3181 acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt cagcacgcca
     3241 tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa gtgcggatta
     3301 ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg cataaaggcg
     3361 cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc cgggttagcg
     3421 ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc ttcttccacg
     3481 cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac gtcgtaacgc
     3541 accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga ggcgatggaa
     3601 tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc cagttgcgcc
     3661 ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg taacttcgct
     3721 ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta attctcaaca
     3781 atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt cagaggcaac
     3841 gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc gacaagttct
     3901 ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg ctggtaacgc
     3961 aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga gttacgcccg
     4021 gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct gtacgggctg
     4081 gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc gacaggcgca
     4141 cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc ctggtattca
     4201 acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag cttgctgtcg
     4261 aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct aaacacggca
     4321 aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg cagcgcgacg
     4381 gcttcctccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag cagtttttcg
     4441 ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt cagttttgct
     4501 gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt ttgcgaggcg
     4561 gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc aagcgcagca
     4621 ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg ggtgggagtt
     4681 aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg
     4741 gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc tgtttctgcg
     4801 ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa cggtgtggat
     4861 taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag caatagtctt
     4921 taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc aatcattaag
     4981 ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc
     5041 ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc acgttattta
     5101 aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg cataaatgtg
     5161 gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa gtcgatccgc
     5221 atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaagcca acttatgagc
     5281 gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc agcgaagttt
     5341 tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg
     5401 taaaagaaac ctgtaccgca ggttgtatct atcgcggcga atgataagag tgtgtcggcg
     5461 gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc tggggcatga
     5521 catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca ccgcttgcgc
     5581 gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg aaatccccgg
     5641 cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac tcctcccgca
     5701 atttcacctg accgcgcctc ttgaacgacg catcactcac gaaagccttt ccctgttaac
     5761 gccggatggt gcaacgacgt tttccagctt acagcccggc ggtgaatcct ggagtgtatt
     5821 acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg tcgaatgcat
     5881 ccccggtgcg acggtggatg cgctgtatga agaaaacggc agggtgtgtg gtgtcatttg
     5941 tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca acagcgtcct
     6001 ggctgaacgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt tggggatcaa
     6061 agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg agaataacga
     6121 aggcgcagcg ttgctgttca gcggcgggat ctgtgatgac ttacccggcg gcgcatttct
     6181 ttatactaat caacaaacgc tctcgttagg gattgtttgc ccactctctt cccttacgca
     6241 aagtcgtgtt ccggcaagcg agctgctggc tcgctttaaa acgcatccgg cagtgcgccc
     6301 gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag aaggtggctt
     6361 gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg atgcgttgcg
     6421 cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga ctggcgcaca
     6481 ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa atctgtttgc
     6541 gctttatcat cacaacgtcg agcgcagcct gctatgggat attctacagc gttatcagca
     6601 tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt taatgcagga
     6661 tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc gccagttatt
     6721 ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta tcaggagtct
     6781 gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat tgttccggct
     6841 gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc aggtcttttt
     6901 tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga gtgtggcacc
     6961 tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc ttccggattc
     7021 ggcatcacct accgctttgg ataaataata aggataattt atgcccctct tacacctgct
     7081 ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac tggctatcga
     7141 ttctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca gtaatatcgt
     7201 taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc tggaaggcgc
     7261 gtcaaataaa gaagtcgtta ttcagttttt gaaactggtg accgaggcgg acggcattat
     7321 cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct gtattcatcg
     7381 cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg cgcaatcgaa
     7441 tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct gggtgacaga
     7501 gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag atgcgcgtaa
     7561 tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct ggacgttagc
     7621 gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat ttgtaggccg
     7681 cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat caggcctaca
     7741 agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg caatctgcgt
     7801 gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg
     7861 cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc
     7921 gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac acctcaggtg
     7981 ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg ttcatcactc
     8041 gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg atttcctggt
     8101 tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct aatgggtcgc
     8161 gagtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgca cccgggacaa
     8221 tgttcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc gcctgacgcg
     8281 ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc
     8341 agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg
     8401 caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa
     8461 agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc agcacctgtt
     8521 ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg ctgaaatcct
     8581 gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca attgcgatat
     8641 tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt tttctgctac
     8701 cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca gttgcagaca
     8761 ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt tccccgcagc
     8821 cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac gcacggcaat
     8881 caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcagtacgct gttcgagggt
     8941 gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc agcccagcat
     9001 ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccggtaat
     9061 tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga caaattccgg
     9121 cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat
     9181 gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg ttaaagacat
     9241 cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt catcagcacc
     9301 gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg ccgcctgcca
     9361 ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat
     9421 tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct ggtgcccata
     9481 gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag gtgacttgca
     9541 ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg ccaagtcgac
     9601 tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag
     9661 gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca
     9721 gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt gccgccagca
     9781 gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca
     9841 gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca
     9901 gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga
     9961 ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa gagaacaagg
    10021 ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca tggggaccaa
    10081 aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg
    10141 gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg
    10201 ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg
    10261 ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata ccgcgatggc
    10321 ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct cccagaccaa
    10381 tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgtaaaa
    10441 acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt
    10501 cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc gagccgccga
    10561 tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac
    10621 caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaatgc
    10681 ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta
    10741 ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc
    10801 ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat
    10861 tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc cacgcgctgc
    10921 cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg
    10981 aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg tggtacttgt
    11041 cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag tacatgttgg
    11101 tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg tcggcgtgcg
    11161 ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg cagctccagc
    11221 agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc acacgttcgg
    11281 cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc agtttgctgt
    11341 ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcgcct
    11401 tcgcaatgcg aggattgcct tcagccataa agatcagcac acatttaccg tcggcaaaat
    11461 gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc
    11521 cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc
    11581 cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat
    11641 agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgggcca gccgcgcggc
    11701 gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc agaccaacga
    11761 ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca
    11821 ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt
    11881 cacgcaacgc attttccagc acctccagcg gaacgccaca ttgcgccgtc gcctgcatat
    11941 tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc accgagtttt
    12001 ccacaacggt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc acaccgttaa
    12061 ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa ccgagtttta
    12121 cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat ttacgaaaac
    12181 ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa
    12241 tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt cct
//
LOCUS       DAPPNJ010000138        11881 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000138 DAPPNJ010000000
VERSION     DAPPNJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.139"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            360..1748
                     /gene="narU"
                     /locus_tag="RPM73_003182"
     CDS             360..1748
                     /gene="narU"
                     /locus_tag="RPM73_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDX6521517.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1830..5570
                     /gene="narZ"
                     /locus_tag="RPM73_003183"
     CDS             1830..5570
                     /gene="narZ"
                     /locus_tag="RPM73_003183"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDX6521518.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5567..7111
                     /gene="narH"
                     /locus_tag="RPM73_003184"
     CDS             5567..7111
                     /gene="narH"
                     /locus_tag="RPM73_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDX6521519.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7111..7806
                     /gene="narW"
                     /locus_tag="RPM73_003185"
     CDS             7111..7806
                     /gene="narW"
                     /locus_tag="RPM73_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDX6521520.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7803..8483
                     /gene="narI"
                     /locus_tag="RPM73_003186"
     CDS             7803..8483
                     /gene="narI"
                     /locus_tag="RPM73_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDX6521521.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8563..9456
                     /locus_tag="RPM73_003187"
     CDS             8563..9456
                     /locus_tag="RPM73_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDX6521522.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWVIEI"
     gene            complement(9552..10397)
                     /gene="nhoA"
                     /locus_tag="RPM73_003188"
     CDS             complement(9552..10397)
                     /gene="nhoA"
                     /locus_tag="RPM73_003188"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDX6521523.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10570..11139
                     /locus_tag="RPM73_003189"
     CDS             10570..11139
                     /locus_tag="RPM73_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDX6521524.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11143..11370)
                     /gene="pptA"
                     /locus_tag="RPM73_003190"
     CDS             complement(11143..11370)
                     /gene="pptA"
                     /locus_tag="RPM73_003190"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDX6521525.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11424..11881)
                     /locus_tag="RPM73_003191"
                     /pseudo
     CDS             complement(11424..11881)
                     /locus_tag="RPM73_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2799 a         3078 c         3096 g         2908 t
ORIGIN      
        1 gacagatcgt tttcctccag ttcatggcct ttttagtgaa taaatcatca ctaattctat
       61 aagtgtctgg tcttgttgat aatatagtta atatgtaacc ctttagattc acgatctgaa
      121 gggttgaaac atgacatacg ttcaaagatt aacccccgtt tgaaggttag cttgttagtt
      181 acatttagta acacatatct ggctacttaa tccccattct gcccccgcgg ttacgctttc
      241 ttatctattc tttcaatcag cacttcaccc aattcatcct taattctaat aactggctat
      301 tgcgccttac ctcctgtttt gtcttttttc attaccaatg tgtgcatgtg aggaacaata
      361 tggcactgca aaatgagaaa aatagtcgtt atcttttacg cgactggaaa ccagaaaatc
      421 cggccttctg ggaaaataaa ggaaaacata ttgctcgaag aaacctctgg atatcagtca
      481 gttgtctact tcttgccttc tgtgtctgga tgctatttag cgcagttacc gttaatctca
      541 ataaaatcgg ttttaatttt actaccgatc aactcttttt attaaccgca ttaccctccg
      601 tttctggcgc attattgcgt gttccctact cctttatggt gcctatattc ggtggacgcc
      661 gatggacggt ttttagtact gcaatcctga ttattccttg cgtctggctc ggaattgccg
      721 tgcaaaatcc gaatactcct tttggggtat ttatcgttat cgctttgcta tgcggttttg
      781 caggtgcaaa ctttgcttcg agcatgggca atatcagttt cttctttcca aaagccaaac
      841 aagggagcgc tcttgggatt aatggcggat taggaaactt aggtgtaagt gtaatgcagc
      901 tggttgcacc gctggtcatt tttgtacctg tatttgcctt tctcggcgtc aatggcgtac
      961 cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc atggatttgg gtaccgctac
     1021 tggcgattgc cacgatcgcc gcctggtcag ggatgaatga tatcgccagt tcacgcgcct
     1081 caattgccga ccagctccct gtcttacaac gcctgcatct ctggctgctg agcctgcttt
     1141 accttgccac cttcggttcg tttatcggtt tttctgcggg ttttgccatg ctggcaaaaa
     1201 cccagttccc ggatgtgaat attctgcgcc tggcgttctt tggcccattt atcggtgcca
     1261 tcgcgcggtc ggttggtggt gctatttccg ataagttcgg cggcgtgcgg gtgacgttga
     1321 tcaactttat ttttatggcg attttcagtg ccctgctgtt ccttacctta ccgggcacag
     1381 gctccggtaa tttcatcgcc ttttacgccg tatttatggg gctgtttctg actgcgggtc
     1441 tgggaagtgg ttctactttc cagatgatcg ccgtcatctt tcgccagata accatttatc
     1501 gggtaaagat gaaaggcggt agtgatgagc aagctcagaa agaagccgtc accgaaacgg
     1561 cggcggctct gggctttatc tcagccattg gcgcagtggg cggctttttt attccgcagg
     1621 cgtttggcat gtcgctcaat atgaccggct ctccggtcgg cgcgatgaaa gtgtttttaa
     1681 tcttctacat cgtttgtgtg ctgctgacct ggctggttta tggtcggcgg aagttcagcc
     1741 aaaaataaga gatcgatgcc tgatgtacaa ccgtgtcgca tccgacaagg catcagatcc
     1801 caattcattc ctggagcagg agtcatgtca tgagtaaact tttggatcgc tttcgctact
     1861 tcaaacaaaa gggcgaaacc tttgccgatg gtcacggaca ggtgatgcat agcaaccgcg
     1921 actgggagga cagctatcgc cagcgttggc agttcgacaa aatcgtgcgt tccacccacg
     1981 gtgttaactg tacaggctcc tgtagctgga aaatctacgt taaaaatggt ctggtgacct
     2041 gggaaatcca acagaccgac tacccgcgca ctcgccctga cctgcccaat catgaacctc
     2101 gcggctgccc gcgtggcgca agttactcct ggtatcttta cagcgctaac cgcctgaaat
     2161 acccgctcat tcgtaaacga ctgattgaac tgtggcgcga agccctcaaa caacacagcg
     2221 atccggtact ggcgtgggca tcgattatga acgatccgca aaagtgcctg agctacaaac
     2281 aagtgcgtgg gcgcggcggg tttatccgct ccaactggca ggaactaaac cagctgattg
     2341 ccgccgctaa cgtctggacc atcaaaacct acggcccgga tcgcgttgcc ggtttctcgc
     2401 cgatcccggc gatgtcgatg gtttcttacg ccgccggaac gcgttatctg tcactgcttg
     2461 gcggcacctg tttaagtttc tacgactggt attgcgacct gccgcccgcc tcgccgatga
     2521 cctggggcga gcaaaccgac gtaccggaat ctgccgactg gtataactcc agctacatca
     2581 tcgcctgggg gtctaacgta ccgcagacac gtacgccgga cgcccacttc tttaccgaag
     2641 tacgctacaa aggcactaaa accatcgcca ttacccctga ctactctgaa gtggccaaat
     2701 tgtgcgacca gtggctggca ccgaaacaag gcactgatag cgccctggcg atggcaatgg
     2761 gccatgtgat tttaaaagag tttcatctcg ataatcccag cgactacttt atcaactact
     2821 gccgccgcta cagcgacatg ccgatgctgg taatgctgga gcctcgcgac gatggtagct
     2881 acgttcccgg gcgcatgatc cgcgcatctg acctggtgga tggactgggc gaaagcaaca
     2941 atccgcagtg gaaaaccgta gcagttaata ccgcaggtga attggtagtg ccgaacggtt
     3001 cgattggttt ccgctgggga gaaaaaggca aatggaatct ggaatccatt gccgccggta
     3061 cggaaaccga attgtcgtta accctgctcg gtcaacatga cgctgttgca ggcgtggcct
     3121 tcccctactt tggcggcatt gaaaatccgc attttcgcag cgtaaaacac aatccggtgc
     3181 tggtgcgcca attgcccgtt aaaaacctga cgttagtcga tggcaacacc tgtccggtgg
     3241 tcagcgttta tgatttggta ctggcgaatt acggcctcga tcgcgggctg gaagatgaaa
     3301 acagtgcgaa agattacgct gaaatcaaac cgtacacccc agcctggggt gagcaaatta
     3361 ccggcgtgcc gcgccagtat attgaaacca tcgcccgtga atttgccgat accgcccata
     3421 aaacgcatgg gcgctcgatg attatcctcg gcgcaggtgt taaccactgg tatcacatgg
     3481 acatgaacta ccgcgggatg atcaatatgc tgatcttctg cggttgtgtc gggcaaagcg
     3541 gtggcggctg ggcacactat gtcggccagg aaaaactgcg cccacaaacc ggctggttgc
     3601 cactggcctt tgcgctcgac tggaaccgac caccgcgcca aatgaacagc acctcgtttt
     3661 tctacaatca ttccagccaa tggcgctatg aaaaagtctc tgctcaggag ttactttcac
     3721 cgctcgccga tgccagtaag tacagcggtc atctgattga tttcaacgtt cgcgccgaac
     3781 gtatgggctg gctaccttct gcgccgcagc tggggcgtaa cccgctcggg attaaagctg
     3841 aagccgacaa ggccggatta tcccccacag aatttaccgc ccaggcgctg aaatcgggcg
     3901 atttacgtat ggcctgcgaa caaccagata gcggcagcaa tcatccgcgt aatttgtttg
     3961 tctggcgctc taacctgctt ggctcctccg gcaaaggcca cgagtatatg cagaagtatc
     4021 tgctggggac cgaaagcggg attcagggcg aggaactcgg tgccagcgac gggatcaaac
     4081 cggaagaagt cgagtggcaa actgcggcga ttgaaggcaa gctcgacctg ctggtgacgc
     4141 tcgacttccg catgtccagt acctgcctgt tctccgatat cgttctgccc accgccacct
     4201 ggtacgaaaa agacgatatg aacacctcgg atatgcatcc gtttattcat ccgctttctg
     4261 cggcggtcga tccggcgtgg gaatcacgca gcgactggga aatctacaaa ggtatcgcca
     4321 aagcattttc gcaagtgtgc gtgggtcatc ttggcaaaga aaccgacgtg gtattacaac
     4381 ccctactgca tgactctccg gcagagctct cacagccgtg tgaagtgctc gactggcgca
     4441 aaggcgaatg cgatctgatt ccaggcaaaa ccgcgccgaa tattgtggcg gtggagcgcg
     4501 actaccctgc tacgtatgaa cgctttacct cgctcgggcc attgatggac aaacttggca
     4561 atggcggtaa agggatttcg tggaatacgc aggatgaaat cgatttcctc ggcaaactca
     4621 attacaccaa gcgtgatggc ccggcacagg ggcgtccgct gattgacacc gccattgacg
     4681 cttcagaagt gattctggca ctggcaccgg aaactaacgg tcatgttgca gtcaaagcgt
     4741 ggcaggcgct gggcgagatc accgggcgcg aacataccca tctggcgctg cacaaagagg
     4801 acgagaagat tcgctttcgc gatattcagg cgcagccgcg taaaattatc tccagcccca
     4861 cctggtccgg tctggaaagc gatcacgtat cctataacgc gggatacacc aacgttcatg
     4921 agttaattcc gtggcgcacg ctgtcgggac gccagcagct ctatcaagat catccgtgga
     4981 tgcgtgcttt tggtgaaagc ctggtggctt atcgcccgcc tatcgacacc cgtagcgtca
     5041 gtgagatgcg ccagataccg ccaaacggct tcccggaaaa agcacttaac ttcctgacgc
     5101 cgcaccagaa atggggcatt cactcaacct acagtgaaaa cctgctaatg ctgacgctct
     5161 ctcgcggtgg accgattgtc tggatcagcg aaacagatgc ccgagaactg accattgtcg
     5221 ataacgactg ggtggaagta ttcaacgcca acggcgcgct gactgcccgc gcggtggtca
     5281 gccaacgtgt accgccgggc atgaccatga tgtatcacgc ccaggaacgc attatgaata
     5341 ttcctggttc ggaagtaact ggcatgcgcg gcggcattca taactcggtc acccgcgttt
     5401 gcccgaaacc aacgcatatg attggcggtt acgcgcagct ggcctggggc tttaactact
     5461 acggcaccgt cggatcgaac cgcgatgagt tcatcatgat ccgcaagatg aagaacgtta
     5521 actggctgga tgatgaaggt cgcgatcagg tacaggaggc gaaaaaatga aaatccgttc
     5581 acaagtcggc atggtgctta acctcgacaa atgtatcggc tgccatacct gttcggtgac
     5641 ctgtaaaaac gtctggaccg ggcgcgaagg catggagtac gcatggttta acaacgtcga
     5701 aaccaaaccg ggcattggtt atccgaaaaa ctgggaagat caggaagagt ggcaaggcgg
     5761 ctgggtccgc gatgtgaatg gcaagatccg tccgcgtctg ggcagcaaga tgggcgtaat
     5821 aaccaaaatc ttcgccaacc cggtggtgcc gcagattgat gattactacg aacctttcac
     5881 cttcgactac gaacatttgc atagcgcacc ggaaggcaaa catatcccta ctgctcgccc
     5941 gcgttcactg attgacggca aacggatgga caaagtgatc tggggcccaa actgggaaga
     6001 actgctgggc ggcgagtttg aaaaacgtgc ccgcgaccgc aacttcgagg ccatgcaaaa
     6061 ggagatgtac gggcagtttg aaaacacctt catgatgtac ctgccgcgcc tgtgcgaaca
     6121 ctgcctcaat cccagttgcg tggcgacctg cccaagcggc gctatctaca aacgcgaaga
     6181 agacggcatt gtgctgattg atcaggataa atgccgtggc tggcgtttgt gcattagcgg
     6241 ttgtccgtac aaaaaaatct acttcaactg gaaaagcggc aagtcagaaa aatgtatctt
     6301 ctgttatccg cgaattgagt caggccaacc caccgtgtgc tcagaaacct gcgtgggtcg
     6361 catccggtat ctgggcgtgc tgctttacga cgccgaccgc attgaggaag cggcgagcac
     6421 cgagcgcgaa gttgacctct atgaacgtca gtgcgaagtg ttcctcgatc cacacgatcc
     6481 ctcagtgatc gaggaagccc tgaaacaagg tattccacaa aacgtgattg aagctgccca
     6541 gcgttcgcct gtctacaaaa tggcgatgga ctggaaactg gcgctaccgc tgcaccctga
     6601 atatcgcacc ctgccaatgg tctggtacgt tcctccgctg tcgccgattc agtcctacgc
     6661 agatgcgggc ggtttgccga aaagcgaagg cgtgctgccc gccatcgaaa gcctgcgtat
     6721 tccggtgcaa tatctcgcca atatgttgag tgccggcgat accggtccgg tactgcgggc
     6781 gctgaaacgg atgatggcga tgcgccacta tatgcgttca caaaccgtgg aaggcgttac
     6841 tgatactcgt gccatcgacg aagtaggcct gagcgtcgcc caggtcgaag agatgtatcg
     6901 ttacctcgcc attgccaact atgaagatcg ttttgtcatc ccgacgagcc atcgggaaat
     6961 ggcgggcgat gccttcgcag aacgcaacgg ctgcggtttt acctttggcg acggttgtca
     7021 cggctcggac agcaaattca acctgttcaa cagtagccgt atcgatgcca tcaacatcac
     7081 cgaagtgcgc gacaaagcgg agggcgaata atgcagatcc tcaaagtgat cggcctgttg
     7141 atggagtatc cggatgagct gttgtgggaa tgcaaggagg acgcgctggc gttgatccgc
     7201 cgcgacgcgc cgatgcttac ggatttcacc cacaacctgc ttaacgcgcc gctgctggat
     7261 aaacaggccg aatggtgcga agtgtttgac cgcgggcgca ccacgtcgct gctgctgttc
     7321 gaacatgttc atgccgagtc ccgcgatcgc ggccaggcaa tggtggacct gctggcggag
     7381 tatgaaaagg tcggcctgca gctggattgt cgggaactgc ccgattatct accgctgtat
     7441 ctggagtatt taagcgtact gcctgacgat caggcgaaag aaggattgct caatgttgcg
     7501 ccgatcctcg ccctgcttgg cggtcgctta aaacaacgcg aggcaccgtg gtacgcgttg
     7561 tttgatgctc tgctgcaact ggcgggaagt atactttcaa gtgacagtgt cacaaaacaa
     7621 gtaaacagcg aagagcgcga tgacacccgc caggcgcttg atgcagtgtg ggaagaggaa
     7681 caggtgaagt ttattgaaga taacgccacg gcgtgtgaca gctcgccgct taatcaatat
     7741 cagcgacgct ttagccagga tgtcgcgccg cagtatgtcg acatcagtgc gggaggtggg
     7801 aaatgattca gtatctgaac gtcttttttt acgatatcta cccgtacatt tgtgcgacgg
     7861 tgtttttcct cggcagctgg ctgcgctacg actacgggca gtacacctgg cgcgcctcct
     7921 caagtcaaat gctcgataaa cgcgggatgg tgatatggtc gaatctgttc catatcggca
     7981 ttttggggat tttcttcggg cacttgttcg gcatgttaac gccgcactgg atgtacgcgt
     8041 ggtttttgcc agtggcagcg aaacaactca tggcgatggt gctcggcggt atttgcggtg
     8101 tgttgacgtt gattggcggc gcaggactgc tgtggcgcag gctgacaaat cagcgcgtgc
     8161 gtgcaacttc cactacgccg gatattatca tcatgagcat tttgctgatc cagtgtctgc
     8221 tggggctaag taccataccg ttttcagcac agtatcctga tggtagcgaa atgatgaagc
     8281 tggtgggttg ggcgcaaagc attgtcactt tccgtggtgg atcgtcagaa atgcttaacg
     8341 gcgtagcgtt cgttttccgc ctgcatctgg tattgggaat gaccatcttc ctgctcttcc
     8401 cgttcaccag attggtgcat gtgtggagcg cgccgtttga gtactttact cgtcggtatc
     8461 agattgtgcg ttcgcggcga taattttcac cctggccgga tagcccttcg ctctccggcc
     8521 acttgcaggc tatgatagtt ctccagccac aggagaacgt taatgaaacc gcaggtgtac
     8581 cacgtcgatg cttttacctc acaaccgttt cgcggcaatt ctgccggtgt tgttttcccc
     8641 gccgataatc ttagcgaagc gcaaatgcag cttatcgccc gcgaattagg tcattcggaa
     8701 actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaaca
     8761 gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg
     8821 ttaggtttag gaaattgcac ggtctggcaa acatcgctgg caggaaaaca tcgcgtgact
     8881 atcgaaaagc acaacgatga ttatcgtatt tcgctggaac aaggtacgcc gggctttgaa
     8941 ccgccgctgg agggtgaaac acgtgaggcg attatcaacg cgcttcatct cactgaggac
     9001 gatattctgc caggcttgcc aattcaggtg gcaacaacag gacactcaaa agtgatgatc
     9061 ccgttgaaac cagaagtgga tatcgacgcc ctttcgcctg atcttgctgc gctgagcgct
     9121 atcagcaaac agattggctg caatggtttc ttcccgttcc agatccgccc aggcaaaaac
     9181 gaaaccgatg gtcgcatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc
     9241 aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc
     9301 aaggtgctgc gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg cgtgattgaa
     9361 gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttcagg cacagcggtg
     9421 attttgtttc atgccgaatg ggtaattgaa atctgaaatc gttgcctgat gcgcttcgcc
     9481 tgtcaggtct acaaccgggc taatgtatgc cggataaggc gtttacgccg catccggcaa
     9541 cctgacataa attattttcc cgcctccgga tgcgtatcaa acgccgccat caccagcgct
     9601 aactcatcca cagtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcctgc
     9661 atcacggcat ataacgacac cacatccggt agatttcgct gctccaccgc gtgcccattt
     9721 tcataatggg taaaatgaaa attggtcagc gttagcttgc caccgtccgg caaatggcgg
     9781 cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca gaaattgccc
     9841 atcacataat cgctttgttg ctgctcgcag agatcaaaac ggtacatcga ctgccaatgc
     9901 tgatgatgat taaactgcaa cacccaatca tcaccctcct gcaacaaccg atactctccg
     9961 tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg gcgcggttag cgtctgccca
    10021 ccgaaaccga catcagcaat ccatttttcc tcttccagtt ccaccaacag caaacgatgg
    10081 gtgcgcggcg gtaatgctgg cggatttgat aacactacgc gccctaacaa gctgcgaacg
    10141 ttaaacccca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa acagtaaccg
    10201 ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg tatttccctc
    10261 ggcagcaaaa cgtcaaggtt ttcaaacgga atggtgcaat tgtgtttcag gtgcaatgca
    10321 cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc aaaatagtga
    10381 ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat aacttggaat
    10441 agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgtttgc aaatcgccaa
    10501 tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc ttcttcgcga
    10561 ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg tctattaaat
    10621 catggcccaa ctgtcatgat caccagtttc gatgagcaat cccagcggcg taacattatg
    10681 gctgcagcct ggtcaatgcc ggtggagttt gaaccgccac gcgtggcgat tgtggtagat
    10741 aaatcgacat ggaccaggga gttgattgaa cgtaacggta agtttggcat cgttatcccg
    10801 ggcgttgcag caactaactg gacgtgggca gtgggaagcg tgtcgggtcg tgaagaagat
    10861 aaatttaatt gctatggcat tccggtggtg aaaggtccgg tgcttggttt gccacttgtt
    10921 gaagagaaat gcctggcgtg gatggagtgc cgcctgctac ctgcgacttc tgcgcaacaa
    10981 aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg cagacgcacg ggtatttgtc
    11041 gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt aggtgctggc
    11101 acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag cattacatgc tgtaaccggg
    11161 tttctttatc aaagcctcca tttggggcgc gatttcggca tcccagatag cttgccaaga
    11221 ttctggttga atctgctgta gagcaatgct tatcgaactg tctttactgt tcagatggcg
    11281 aataataacg tcggtaatat ctgcagcaag tgctgctttt tgttgttcgt ccagttcacg
    11341 cggaaagcat ttaatgtcga tgtgcggcat gattgtgctc cttttgaaag atgtcagtaa
    11401 aattaacaca cattgtgaat tttcagtcct ttccattcaa acgtgaaatc aataagttca
    11461 tcagggacat cgcaaacaat atgaatacgg atttcttctc tgccgtgact cttttcacaa
    11521 attgcgtaac tgtcatgctc tggattattt aattctttca gcggaagttt ttcctcaaag
    11581 gctttattta gccacccctg gtttcctttc accgcgaata aataatcacc tccctgtttt
    11641 tgtatcttct ctgctatatc tttctggcaa cccatcgcat cagttgtgat gatttttcct
    11701 ttaatatcca gcatgttaag aagttctggg atagctgtaa tctcattaga tttatcatcc
    11761 gtcttgatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
    11821 gctcccctgc ggcgactctt atcataagaa tgccggagcg tttttccatc aattgcaatg
    11881 a
//
LOCUS       DAPPNJ010000139        11844 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000139 DAPPNJ010000000
VERSION     DAPPNJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.140"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            32..466
                     /gene="slyA"
                     /locus_tag="RPM73_003192"
     CDS             32..466
                     /gene="slyA"
                     /locus_tag="RPM73_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDX6521526.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(513..980)
                     /gene="slyB"
                     /locus_tag="RPM73_003193"
     CDS             complement(513..980)
                     /gene="slyB"
                     /locus_tag="RPM73_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDX6521527.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1254..2363
                     /gene="anmK"
                     /locus_tag="RPM73_003194"
     CDS             1254..2363
                     /gene="anmK"
                     /locus_tag="RPM73_003194"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDX6521528.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2461..2790
                     /gene="mliC"
                     /locus_tag="RPM73_003195"
     CDS             2461..2790
                     /gene="mliC"
                     /locus_tag="RPM73_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDX6521529.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2849..3505
                     /gene="pdxH"
                     /locus_tag="RPM73_003196"
     CDS             2849..3505
                     /gene="pdxH"
                     /locus_tag="RPM73_003196"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDX6521530.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3634..4908
                     /gene="tyrS"
                     /locus_tag="RPM73_003197"
     CDS             3634..4908
                     /gene="tyrS"
                     /locus_tag="RPM73_003197"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDX6521531.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            4967..5830
                     /gene="pdxY"
                     /locus_tag="RPM73_003198"
     CDS             4967..5830
                     /gene="pdxY"
                     /locus_tag="RPM73_003198"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDX6521532.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5874..6479)
                     /gene="gstA"
                     /locus_tag="RPM73_003199"
     CDS             complement(5874..6479)
                     /gene="gstA"
                     /locus_tag="RPM73_003199"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDX6521533.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6585..8087)
                     /gene="dtpA"
                     /locus_tag="RPM73_003200"
     CDS             complement(6585..8087)
                     /gene="dtpA"
                     /locus_tag="RPM73_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDX6521534.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8698..9333)
                     /gene="nth"
                     /locus_tag="RPM73_003201"
     CDS             complement(8698..9333)
                     /gene="nth"
                     /locus_tag="RPM73_003201"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDX6521535.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9333..10028)
                     /gene="rsxE"
                     /locus_tag="RPM73_003202"
     CDS             complement(9333..10028)
                     /gene="rsxE"
                     /locus_tag="RPM73_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDX6521536.1"
                     /translation="MSEIKDVIVQGVWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFATKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(10032..10652)
                     /gene="rsxG"
                     /locus_tag="RPM73_003203"
     CDS             complement(10032..10652)
                     /gene="rsxG"
                     /locus_tag="RPM73_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDX6521537.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(10656..11714)
                     /gene="rsxD"
                     /locus_tag="RPM73_003204"
     CDS             complement(10656..11714)
                     /gene="rsxD"
                     /locus_tag="RPM73_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDX6521538.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11715..>11844)
                     /locus_tag="RPM73_003205"
     CDS             complement(11715..>11844)
                     /locus_tag="RPM73_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HDX6521539.1"
                     /translation="LEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         3046 a         2967 c         3000 g         2831 t
ORIGIN      
        1 atcttagcaa gctaattata aggagatgaa attggaatcg ccactaggtt ctgatctggc
       61 acggttggtg cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc tggagttaac
      121 acaaacccat tgggttacgt tacacaatat ccatcagtta cctccagacc agtcgcaaat
      181 tcaactggca aaagcgattg gcatcgagca gccatcactg gtccgtactc tggaccaact
      241 ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg ctaaacgtat
      301 taaactgacg gaaaaggcag agccactgat cagcgaaatg gaagctgtta ttaacaaaac
      361 ccgcgcggaa atattacatg gcatctccgc agaggaactg gagcaactga ttacgctcat
      421 cgcaaaactt gagcataata tcattgagtt acaggccaaa gggtgaaatg aagggggcgt
      481 gtggtcagta acctgaccac acgcaaactt atttagcgcg gagaaacggt cacctgactg
      541 ccattgctgg ccagtacgac acgttggccc ggagagaaac gagtgttgcc ttgtttctgt
      601 accaccatga tggtattacc atcgtcttta cgaatttcca gctcgacacc ctgcgttttg
      661 ttcattgcac tctgtacgcc ctgaccagct acgccacctg caacagcgcc tgctgcagta
      721 gccagagaac gcccggttcc gccaccaaca gtattcccca ggaaaccacc aagaacagca
      781 ccgccaattg caccgataac gttggaatca tcaccgccct gaatctgtac cggacgtacg
      841 ttaacgatgg tgccatagct gacattctgt acttgtttcg cttcagaagc ggtataaaca
      901 tcccctgaca gggtgtcgtt attaacacaa ccgacaagag acagacctac cattgaaaca
      961 accaatacgc gtttaatcat tgaaaaatct cctgttcacc atgaaacgcc acgcaagcat
     1021 ccctcatggt caaagtatat ggcatatgtg aatccatggt tacatgttct accaaatatg
     1081 agcaaatcat aatgaaagta ggatcaacca attataaaca aaacattcat gatgattatt
     1141 gatttgaaga taatgtttta ctaccaacgg taaaaatcgg tcattccgca tggtattcac
     1201 gaaaagttta tgtttgagtg gtgcgcaaac gtcagattga aaaaggatga actatgaaat
     1261 cgggccgctt tattggcgtt atgtcaggca ccagccttga tggtgttgat gttgtgttgg
     1321 cgacaataga tgaacaccgg gtcgcacagc tggcaagctt gagctggccg atcccggtgt
     1381 ctctgaaaca ggctgtactg gatatttgcc agggccagca gcttacactt tcgcagtttg
     1441 gacagcttga tactcaactc gggcgacttt ttgctgatgc ggtcaatgcc ttgcttaagg
     1501 aacaaaacct gcaggcgaga gatatagttg cgatcggctg tcacggtcaa accgtctggc
     1561 atgaaccgac gggcgtggca ccacacactt tacagattgg cgataacaat caaattgtgg
     1621 cacgcaccgg aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac
     1681 aaggcgcacc gctggtacct gcgttccatc atgccttgct ggctcactca accgagcgac
     1741 gaatggtgct caatattggc ggcatcgcca atctgtcact gctcattcct gggcagccgg
     1801 ttggcggcta cgataccggt cctggtaaca tgctgatgga tgcctggatc tggcgtcagg
     1861 ccggtaaacc ttacgataaa gatgccgagt gggcacgggc gggtaaagtt attctcccac
     1921 tgctgcaaaa tatgctcagc gacccatact tctcgcaacc tgcaccgaaa agcaccggac
     1981 gcgaatactt taactacggc tggctggagc gccatttacg ccattttccg ggtgttgatc
     2041 cccgtgatgt tcaggccaca ctggcagaac tcaccgccgt gaccatttct gaacaagttt
     2101 tgttgagcgg tggctgcgaa cgattgatgg tatgtggtgg aggtagtcgt aatccgctac
     2161 tcatggcgcg tctggcggca ttactgccag gcacagaagt caccaccacc gatgccgttg
     2221 gcattagtgg cgatgacatg gaagcattgg ctttcgcctg gcttgcctgg cggacgctgg
     2281 cgggattacc aggaaatctg ccttccgtca ctggcgcaag ccaggagacg gtactggggg
     2341 ctattttccc tgctaacccg tgatatcaga gttaactgaa tttttcttac cgcgtctgcc
     2401 gttacacttg gaacgttagg gagggcgtat tgccctccag accaggaaag tcttcgggaa
     2461 atgtctatga aaaaactgtt actgatctgt ttgccggtgc ttctcaccgg ctgtagcgcg
     2521 ttcaatcaac tggttgagca catgcaaacc gctacgctgg aataccagtg tgatgaaaaa
     2581 ccgttgacgg tcaaactgaa taatccgcgc caggaagtca gttttgttta cgataatcaa
     2641 ctactgcatc tcaaacaggg catttcagcg tctggtgcac gttacactga cggaatctat
     2701 gttttctggt cgaaaggcga tgaagcgact gtctataaac gcgaccgcat cgtattgaat
     2761 aactgtcagt tacaaaatcc acagcgttga gatttttcca ggggcggcgc acaatagcgt
     2821 cacccactga caatccgtaa agaaaaccat gtctgataac gacgaattgc agcaaatcgc
     2881 gcatctgcgc cgtgaataca ccaaaggcgg gttacgccgt cgcgatcttc ccgccgatcc
     2941 attaaccctt tttgaacgtt ggctctctca ggcgtgtgaa gccaaactgg cagaccctac
     3001 cgcgatggtg gtcgctaccg tggatgaaca tgctcagcct tatcaacgca tcgttttact
     3061 caaacattac gacgaaaaag gcatggtgtt ttacaccaac ctcggtagcc gtaaagcaca
     3121 tcaaattgaa aataatccgc gcgttagcct gctgttcccg tggcataccc ttgaacgcca
     3181 ggtgatggtg atcggtaaag cagaacgact ttcgactctc gaagtgatga aatattttca
     3241 tagccgcccg cgtgatagcc agattggtgc atgggtttcg aagcagtcca gtcgcatttc
     3301 tgcccgcggt atccttgaaa gtaaattcct ggagctgaag cagaagtttc aacagggcga
     3361 agtgccattg ccgagctttt ggggcggttt tcgcgtcagc cttgaacaga ttgagttctg
     3421 gcagggcggt gagcatcgcc tgcatgaccg ctttttgtac cagcgagaaa atgatgcgtg
     3481 gaagattgat cgtcttgcac cctgaaaaga tgcaaaaatc ttgctttaat cgctggtact
     3541 cctgattctg gcactttatt ctatgtctct ttcgcatctg gcgaaaagtc gtgtaccggc
     3601 aaaggtgcag tcgttatata catggagatt ttgatggcaa gcagtaactt gattaaacaa
     3661 ttgcaagagc gggggctggt agcccaggtg acggacgagg aagcgttagc agagcgactg
     3721 gcgcaaggcc cgatcgcgct ctattgcggc ttcgatccta ccgctgacag cttgcatttg
     3781 gggcatcttg ttccattgtt atgcctgaaa cgcttccagc aggcgggcca caagccggtt
     3841 gcgctggtag gcggcgcgac gggtctgatt ggcgacccga gcttcaaagc tgccgagcgt
     3901 aagctgaaca ccgaagaaac tgttcaggag tgggtggaca aaatccgtaa gcaggttgcc
     3961 ccgttcctcg atttcgactg tggagaaaac tctgctatcg cggcgaacaa ctatgactgg
     4021 ttcggcaata tgaatgtgct gaccttcctg cgcgatattg gcaaacactt ctccgttaac
     4081 cagatgatca acaaagaagc ggttaagcag cgtctcaacc gtgaagatca ggggatttcg
     4141 ttcactgagt tttcctacaa cctgttgcag ggttatgact tcgcctgtct gaacaaacag
     4201 tacggtgtgg tgctgcaaat tggtggttct gaccagtggg gtaacatcac ttctggtatc
     4261 gacctgaccc gtcgtctgca tcagaatcag gtgtttggcc tgaccgttcc gctgatcact
     4321 aaagcagatg gcaccaaatt tggtaaaact gaaggcggcg cagtctggtt ggatccgaag
     4381 aaaaccagcc cgtacaaatt ctaccagttc tggatcaaca ctgcggatgc cgacgtttac
     4441 cgcttcctga agttcttcac ctttatgagc attgaagaga tcaacgccct ggaagaagaa
     4501 gataaaaaca gcggtaaagc accgcgcgcc cagtatgtac tggcggagca ggtgactcgt
     4561 ctggttcacg gtgaagaagg tttacaggcg gcaaaacgta ttaccgaatg cctgttcagc
     4621 ggttctttga gtgcgctgag tgaagcggac ttcgaacagc tggcgcagga cggcgtaccg
     4681 atggttgaga tggaaaaggg cgcagacctg atgcaggcgc tggtcgattc tgaactgcaa
     4741 ccttcccgtg gtcaggcgcg taaaactatc gcctccaatg ccatcaccat taacggtgaa
     4801 aaacagtccg atcctgaata cttctttaaa gaagaagatc gtctgtttgg tcgttttacc
     4861 ttactgcgtc gcggcaaaaa gaattactgt ctgatttgct ggaaataatg cattaagtgg
     4921 aaagggggag tgagaaatca ctccccctgg tttttataca gggaacatga tgaaaaatat
     4981 tctcgctatc cagtctcacg ttgtttatgg tcatgcgggt aacagtgcgg cagagtttcc
     5041 tatgcgccgc ctgggcgcga acgtctggcc gctgaacacc gttcaatttt ctaatcacac
     5101 ccaatacggc aaatggactg gctgcgtgat gccgcccagc catttaaccg aaattgtgca
     5161 aggcattgcc gccattgata aattacacac ctgtgatgcc gtgttaagtg gctatctggg
     5221 atcggcggag cagggtgaac atatcctcgg tatcgtccgt caggtgaaag ccgcgaatcc
     5281 gcaggcgaaa tatttttgcg atccggtaat gggtcatccg gaaaaaggct gtatcgttgc
     5341 accgggtgtc gcagagtttc atgtgcggca cggtttgcct gccagcgata tcattgcgcc
     5401 gaatctggtt gagctggaaa tactctgtga gcatgcggta aataacgtcg aagaagcggt
     5461 tctggcagcg cgcgaactca ttgcgcaagg gccacaaatt gtgttggtta aacacctggc
     5521 gcgagctggc tacagccgtg accgttttga aatgctgctg gtcaccgccg atgaagcctg
     5581 gcatatcagc cgtccgctgg tggattttgg tatgcgccag ccggtaggtg ttggtgatgt
     5641 gacgagcggt ttactgctgg tgaaactgct tcagggggca acgctgcagg aggcgctgga
     5701 acatgtgacc gctgcagtct acgaaatcat ggtgaccacc aaagcaatgc aggaatatga
     5761 gctgcaagtg gtggctgctc aggatcgtat tgccaaacca gaacattact tcagcgcaac
     5821 aaagctctga aatatctcat ttcggcccac aacgacctgt gggccgatgg atattacttt
     5881 aagccttccg ctgacagcgc gtcttgtact tccggacgtt cagccatacg ttgcataaat
     5941 gctgcaatgt gctctaaccc ttccagattc agtttcaccg cgtatgccca gcgcagaacc
     6001 gtaaacagat aggcatcagc aattgtaaat ctttgcccgc agatccagtg ctcatccttc
     6061 agtgcctcgt tcacatattg cagcttcttc tccagctgcg cgcgaactgt cggtttgtac
     6121 tcttccggtg tatcagggcg aaacagaggt gtgaaacctt tatgcagctc ggtggcgatg
     6181 taattcagcc attcgatggt tttatagcgg gaaatactgt ttaccggtgc cagcaactgg
     6241 cggtcgggga cgctgtcggc aagatactgc ataatcgcta cgccttccgt cagcaaagta
     6301 ccgtcatcca gcagcaatgc aggcacctgc cccttagggt taacggcaaa gtaatcgtca
     6361 ccgttttcga gacgtttttt cattaaatcc acactgacga gggtaaaatc ctttccgctc
     6421 tcacgcaggg tgatatggga agcgagagag caggcacccg gtttgtagaa caatttcatc
     6481 gataactcct tttgtctgtg gtttaaagta tggtagtgcg ttgctgaaca aaaaaaaagc
     6541 cgctaacgaa aagttagcgg cttgtaaaag agtttccctg aattttacgc tacagcagct
     6601 ttcgccgctt tgtctgcagc gtcatcctgc gtcatacggt gcagtttagg cgcggtcagc
     6661 agcatcagta ctgcaataat ggcagtagcg acaccaatct gcaagaatac gcgaccataa
     6721 acttccagtg acatcagcgg gtcggtaacg ttatccggca cagccatcat acccgcaaca
     6781 taaccaccaa tcaggtttgc accggcagtg gtcaggaacc agctacccat aatgaagccc
     6841 atcagacgct gcggaacgag ttgtgcaacc attgccagac ccagaccaga gatcatcagt
     6901 tccccgatgc tctgcaggcc atagcttgcg accagccagc ttacagacac gataccagcg
     6961 tcagacgcga atttcgcacc cagcggcaga atcaggaacg caccagaaca catcaccatg
     7021 ccgattgcaa acttggttgg catcggcagg gtatcgccca tcttgttata gatagcggcc
     7081 agaatcggac taccgatgat gatccagaac gggttcagtg cctgatactg ctcaggttct
     7141 acggccagac ccagaatgga gtgctcaacg ttacgaatcg caaagaagtt cagtgacgtt
     7201 ggcatctggc tgtacagcac gaagaagata atggcttcga gcatcaggat gaaggcaacg
     7261 atcattttac gacgcgcagc acctttcatc gcgaaggctt ctttaccgaa gataaccacg
     7321 ataccgaagg caacaacgcc cagcgccata cgcgcaactt cctgattgtg cagcagccag
     7381 gtggcgatag cgatcagtgc cacaacacca ataatggtca gcagcaggtt acggtagttg
     7441 ataggctcga agtctggttt tgaaccgtac tgtttaaccc agcgttggca gaaggcgaag
     7501 ttaacgatag tgatcagcag gcctacaacg ctcaacgcaa acgcaacact ccagccgtat
     7561 ttcgcggcca gccacggcgt agcaatcata gagaagaaag agccgatgtt gacggacatg
     7621 tagtacatgg tgaatgcacc gtccagacgc gggtcgtttt tctcatagca tgtagaaagc
     7681 agagaagacg ggttagcttt aaacaggccg ttaccgaccg caatagccgc catacccata
     7741 taaacgatac cggcgtcgtg accagaccag gcaaccagag cataaccaat cgccagcaca
     7801 atagcgccga gcataattac gcgtttagta cccagtacct tgtcacctaa ccagccgccg
     7861 atagcgacca gaccataaac cagggcacta aaggaagaga aaagggtgat tgaatccgct
     7921 tcagacatac ccagttgttt aaccaggtaa acagccataa ttccttgtag gccgtaataa
     7981 ccaaaacgtt cccataactc aatcgagaag atgagataga acgccttcgg ttgtttgaaa
     8041 gcgttcaaac tgacgctttc agttggtttt tggtttgcag tggacacttt tacctctttt
     8101 tttacatccc atattaacgg gggtgctttc tcgcgttatg tctaaaaaac atacgcgcaa
     8161 ttgttatagt gggaggggaa acggcaggta atgttcacta tcttgcactt tcagacaagt
     8221 cctttgtaat aatctgttac atataactga ggcggggaat tcaaccattc cgggaaatgt
     8281 tatccagcgt taaatttttc aaaaaacgta acggcagatt ttttcactgc actgagaggg
     8341 gggtgatggc ttaacggcga tatgttctgc tatttagcgt ttttactagt gagatagtcc
     8401 agtttctgaa aaatagccag tgtaatgttt tgtaggtcaa tatttgttgc gtgtgtaaat
     8461 ttaaatgatt ttaacctgat tcttgcaatg tttttacatt gcgacagcat cgtgatcttg
     8521 atcacgaatg aatagaaaat ttcgctatta aaaaaacaat taacctgcat ttttggttat
     8581 ccgcaaacgg attacctgat gataacgcta agcaatggca tcattgacgt taaaaagatg
     8641 ttttaaaaga ggataaagaa aggttatcaa tggggtaatc ggtgttaccc cttttcttca
     8701 gatgtcaact ttctctttgt attcacaaag atcttcaata atacaagagc cacagcgggg
     8761 cttgcgggca atgcaggtat aacgcccgtg caggatcaac caatggtggc agtcgacttt
     8821 aaactctgcc ggaaccactt tcagtagctt ttcttctacc tgttcgacgt ttttccccgg
     8881 cgcaaattga gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca
     8941 gccgaatgca gtgtttaata cgacgttggc tgttttacga cctacgccgg gcagggcttc
     9001 aagcgcagca cgatcttccg gaacctcgcc attatgctgc tccagcaaga tacggcaggt
     9061 tttgatgata ttttctgctt tgctgttata aagcccaatc gttttgatat aggttttcac
     9121 cccttcaacg cccagttcaa gcatcgctgc aggcgtattc gccaccgggt agagtttcgc
     9181 cgtcgcctta ttaacactga catcggtcgc ctgagcggaa agcagtacgg caatcagcaa
     9241 ttcaaaaggc gaactgaaat taagctcggt ggtgggatga ggattgttct cacgcaggcg
     9301 agtgaggatc tccaggcgtt ttgctttgtt catcagacat tccctgtttc accgtttggc
     9361 aatgcacgtt cggcagttgc ttcagcacgg cgctttttca ttctttcatc aatcaggtat
     9421 tttcctgcca gcatcagtcc caggccaata aatgcacctg gaggcagcat cgccagcagg
     9481 aaaggggagt cggtgtggaa aatttccacg cgtaatactt ttgcccagct acctaacagc
     9541 gcatctgcac cgtcaaacaa tgtgccattg ccgataattt cgcgtagtga acccagcacg
     9601 aacatggcgc aggttgcgcc cataccaatt gaaaagccgt ccagtgccga aagcgccgga
     9661 ccttttttgg tggcgaaggc ttcagcgcgg cccacaacga tacagttagt gacaatcagc
     9721 ggaataaaaa tccctaatga ttgatacagg ccaaaggcgt aggcgttgat cagcatctgt
     9781 acagcgctga ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat ctcggctggc
     9841 gtccagtgac gcagcgtcga aatggtcagg ttggtcagcg tcagtaccag cgtagtcgca
     9901 agtcctaaac ccagagcgtt agtggcagtg gacgtgaccg ccaacagagg acaaaggccg
     9961 agcaactgga ccagcgcaga gttgtttttc cacaccccct gaacaataac gtctttaatt
    10021 tcgctcacgc tttattctcc acaggcagga agttgagaaa gttgtgccgg taacgtctga
    10081 gcgtacaatc cggcgcgttt taccgcatta accaccgcgc ggggagtaat cgtcgcgccg
    10141 gtgaactggt cgaaatcacc accatctttc ttcaccgccc agtgcgcatc atccgcacca
    10201 ctgatttttt tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc gattttatcg
    10261 ccaagccctg gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt gccgttaaaa
    10321 tcggctccca ccagcagctg aatcgcaccg gaatagccat ctggcgcggt tgcttccaga
    10381 acggcggcta ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc acctttacct
    10441 aactctggcg cagttaccag atagcaactc tgtgccagcg cattgttata gcgttcggct
    10501 ggcagcacct gatcaaataa tgccttttgt tgcagactgg cctgttcagc aatcgtcgtt
    10561 ttggtcatct ggttgatggc cgcagttaac cctgttgaac ccgctgcaaa tagcgctaac
    10621 gtaatgccgt gttttcggat agttttcagc atggtttacc ctttgcgatg gccgtagacg
    10681 cgcggacgcg tgtagtaatc gatcagagga accgtgatgt tcgccagcag gacggcaaaa
    10741 gccacgccgt caggatagcc gccgaaactg cggatcaacc agactaataa gcccgccagc
    10801 gcgccgaaaa taagacgacc acgattggtc gtagaagcgg taaccgggtc agtcaaaata
    10861 aagaatgcgc cgagcatggt cgcaccagac agcagatgaa tttgcggtgc tgccagtgtt
    10921 tctggtgaga acaaccagcc caacgttgcg cataacgcca gcgttactaa gaagctgagg
    10981 ggaatatgcc agcgaatcgc tttctgccat agcaaccaca ggccgccagc cagccaggcg
    11041 agatttaccc attgccaacc agcgcccgcc agaataccgc tgtagatcgg atattgcata
    11101 atctgttcaa ccgaatgacc ggcacggaca gaggttttaa atgtatccag cggtgtcgcc
    11161 tgactaatgc catcaatacc taagcgtagt gtgttcatat caccaccact ggcggtatgt
    11221 ccgctgaaaa taacctgtat ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt
    11281 ggcggtaacc agctggtcat ctgcaccggg aaggagatca gtaagaccac ataaccaatc
    11341 attgccggat taaacgggtt ttgccccaga ccgccataca actgtttagc gatgatcacc
    11401 gcaaacaccg tacccagcac gaccatccac catggcgcga gggggggaat acttaccgcc
    11461 agcaataagc ctgtcagcaa tgctgagtta tctttcaacg ttgcggctac cgactgcttg
    11521 cgtagtttga gtacgagagc ttcggctaac agagcactaa ccgatgccaa caggatctga
    11581 acgagagtac cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc
    11641 aacaccaaca gcataatgcg cgatgtctgg cgctggttat gggtataagg ggagctagct
    11701 attctgaata ccatttagtc ctcgtttaca accttctgct gggcggcttt tttggcctga
    11761 gcgcgggcaa tagccgcggc aactgccgct ttgcgcggat caacctgttc ttctggttct
    11821 gcgttagtct gttgctgttc cagc
//
LOCUS       DAPPNJ010000140        11729 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000140 DAPPNJ010000000
VERSION     DAPPNJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.141"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(11..292)
                     /locus_tag="RPM73_003206"
     CDS             complement(11..292)
                     /locus_tag="RPM73_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDX6521540.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(555..1664)
                     /gene="apbC"
                     /locus_tag="RPM73_003207"
     CDS             complement(555..1664)
                     /gene="apbC"
                     /locus_tag="RPM73_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDX6521541.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1796..3829
                     /gene="metG"
                     /locus_tag="RPM73_003208"
     CDS             1796..3829
                     /gene="metG"
                     /locus_tag="RPM73_003208"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDX6521542.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3970..7764
                     /locus_tag="RPM73_003209"
     CDS             3970..7764
                     /locus_tag="RPM73_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDX6521543.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDAHTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAEFSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7774..11406
                     /locus_tag="RPM73_003210"
     CDS             7774..11406
                     /locus_tag="RPM73_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDX6521544.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAGE
                     MMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATEPACYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPIARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     IFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPVKDNWAFLALSLFGDESTARDLTTQILGWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11467..>11729
                     /locus_tag="RPM73_003211"
     CDS             11467..>11729
                     /locus_tag="RPM73_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521545.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETV"
BASE COUNT         3050 a         2992 c         2909 g         2778 t
ORIGIN      
        1 ggttgccaaa ttaatcgtag cgatttaaca cgattcgacg tttgctatcg ctacctgcaa
       61 caaccacttc tgtcgttaca cctttgacgg gcgaagaaaa cagatgacgg gtgtcaacat
      121 tatacgttaa ctgaccacaa tgctgttgtg gttgactatt aaccatagcc atactgcatg
      181 ttgggggact gatcctgcca ttaatcgtta atgtggcagt ctctgaagag aatgcgggag
      241 aagccaatcc ataggcaaga aaaataattc ctgacagcca atacttattc attgaacgtt
      301 atccctgtag taaaggttat gcctggatag aatgagtgca taacaaacta tagctgtaca
      361 tccactacac agccacgaag gatgataatg aagcattgcc tgtatgatca atcgactttg
      421 tagagtttcg gacgaaggtc cgcagaatat tcgcagtatt aaataagtgt tcattaatgc
      481 ggcaaaaact aatacaccgc atcaatgtaa catctctgcg gtgtatgaag aataaccaga
      541 atggttaata ggcgttagac cgcgcggaag gaaatctcgc ctggaatgac ttcaccctgc
      601 cagtagagct gagctgcaac gcggtcagcc agctggcggt agatagcggt aaattcgctc
      661 tccggacggc tgataaccgt tggcgtgcct ttatcgagat cctcacgtaa ggagatgtgt
      721 aacggcatct gccccagcag ctgtgtgtgg tatttttcag ccagtttctc agcaccaccg
      781 gtaccgaaaa tcggttcatg gtgaccacag ttactgcaaa tatgcacact catgttctcg
      841 acaataccaa gcaccggaac ctcgactttc tcgaacatca caatgccttt tttcgcatcg
      901 atcaacgcga tgtcctgcgg ggtggtgacc acgactgcac cagttactgg aatgttttgc
      961 gccagcgtca attgaatgtc accggtgcct ggcggcatat caagcaccag atagtcgaga
     1021 tccggccaca aggtttcctg caacatctgc attaacgcct tgctcgccat cggtccacgc
     1081 cacaccatcg cattgtcgtc agtgaccaga taaccaattg aattggttgc caggccatga
     1141 gacataatcg gtgccatgtg ggtaccgtcc ggcgacgttg gacgctgatt ttctgcgccc
     1201 agcatggtgg gaattgatgg gccgtagata tcggcgtcca gaatgccgac tttcgcccct
     1261 tccgccgcca gtgccagcgc caggtttacc gccgtggagg atttacccac gccgccttta
     1321 ccggagctga ccgcaatgat gttcttcaca ccattaatac ccggctgatt cttcacgcgt
     1381 tttaaggtgg cgatattgtg cgacagtttc cagtcgatag ctttcgcccc ggtgatacgc
     1441 agcagttcgg cgctacattg ctcttttaac tcttcaaacg cgctatgcca gacgaatggc
     1501 ataaccagtt ccacatgcaa cgtgtcgtcc atccaggcga catggtgcaa cgctttcagc
     1561 gtggtgaggt tatgcttcag ggttgggtgc tgaaaattgg ccagtgtccc ggcgaccatt
     1621 gcgcgcaagg cttctggtga tttggcctgg gattgttcgt tcatcccgac tccttttttg
     1681 tatagataaa ccatcagctg atagtttacc tgaagaatat agagaagtac ttacttaaca
     1741 ttttcccatt tggtactatc taaccccttt tcactattaa gaagtaatgc ctactatgac
     1801 tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg tacgctaacg gctcaatcca
     1861 cctcggccat atgctggagc acatccaggc tgatgtctgg gtccgttacc agcgaatgcg
     1921 cggccacgag gtcaacttca tctgcgccga cgatgcccac ggtacaccga tcatgctgaa
     1981 agctcagcag cttggtatca ccccggagca gatgattggc gaaatgagtc aggagcatca
     2041 gactgatttc gcaggcttta acatcagcta tgacaactat cactcgacgc acagcgaaga
     2101 gaaccgccag ttgtcagaac ttatctactc tcgcctgaaa gagaacggtt ttattaaaaa
     2161 ccgcaccatc tctcagctgt acgatccgga aaaaggcatg ttcctgccgg accgttttgt
     2221 gaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac ggcgataact gcgaagtctg
     2281 cggcgcgacc tacagcccga ctgaattgat cgagccgaaa tcggtggttt ctggcgctac
     2341 gccggtaatg cgtgattctg aacacttctt ctttgatctg ccctctttca gcgaaatgtt
     2401 gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg gcaaataaaa tgcaggagtg
     2461 gtttgaatcc ggcctgcaac agtgggatat ctcccgcgac gcgccatact ttggttttga
     2521 aattccgaac gcgccgggca aatatttcta cgtctggctg gacgcaccga ttggctacat
     2581 gggttctttc aagaatctgt gcgacaagcg cggcgacagc gtaagcttcg atgaatactg
     2641 gaagaaagac tccaccgccg agctgtacca cttcatcggt aaagatattg tttacttcca
     2701 cagcctgttc tggcctgcca tgctggaagg cagcaacttc cgcaagccga ccaacctgtt
     2761 tgttcatggc tatgtgacgg tgaacggcgc aaagatgtcc aagtctcgcg gcacctttat
     2821 taaagccagc acctggctga atcattttga cgctgacagc ctgcgttact actacactgc
     2881 gaaactctct tcgcgcattg atgatatcga tctcaacctg gaggatttcg ttcagcgtgt
     2941 gaatgccgat atcgtgaaca aagtggttaa cctggcctct cgtaatgcgg gctttatcaa
     3001 caagcgtttt gacggcgtgc tggcaagcga attggctgac ccgcagttgt acaaaacctt
     3061 cactgatgcc gctgaagtga ttggtgaagc gtgggaaagc cgcgaatttg gtaaagcagt
     3121 gcgcgaaatc atggcgctgg ctgatctggc taaccgctat gtcgacgagc aggctccgtg
     3181 ggtggtggca aaacaggaag gccgcgatgc cgacctgcag gcaatttgct caatgggcat
     3241 aaacctgttc cgcgtgctga tgacttacct gaagccggta ctgccgaaac tgaccgagcg
     3301 cgcagaagca ttcctcaata cagaactgac ctgggatggt atccagcaac cgctgctggg
     3361 ccacaaagtg aatccgttca aagcgctgta taaccgcatc gatatgaagc aggttgaagc
     3421 actggtggaa gcgtctaaag aagaagtgaa agcagctgcc gcgccagtta ctggcccact
     3481 ggcagatgat ccgattcagg aaaccatcac ctttgacgac ttcgcaaaag ttgacctgcg
     3541 cgtggcgctg atcgaaaacg cagagtttgt tgaaggttct gacaaactgc tgcgcctgac
     3601 actggatctc ggcggtgaaa aacgcaatgt cttctccggt attcgttctg cctacccgga
     3661 tccgcaggca ctgatcgggc gtcacaccat tatggtggcc aacctggcac cgcgtaaaat
     3721 gcgctttggt atctccgaag gcatggtaat ggctgctggt cctggcggga aagatatctt
     3781 cctgttaagc ccggatgctg gtgctaaacc aggccatcag gtgaaataat ccccctttaa
     3841 ggcgctgcat cgacagcgcc ttttctttat aaattcctaa agttgttttc ttgcgatttt
     3901 gtctctctct aacccgcata aatactggta gcatctgcat tcaactggat aaaattacag
     3961 ggatgcagaa tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcagtt
     4021 gagcaacagg gaaacgagtt gcatatcagt tggggaaaag ttggcaccaa agggcaaagc
     4081 cagataaaaa gtttttcaga tgctgcggca gtggcaaaag cggagcttaa gctgattgcg
     4141 gagaaggtga agaaggggta tgtggagcaa gcgaaggata attctttgca accttcccaa
     4201 acggtaacgg gctctctcaa ggtagcggac ttatccacca ttattcagga acaaccctct
     4261 ttcgtagcag aaacccgtgc gcctgacaaa aatacagatg ctgttttacc gtggctggcg
     4321 aaagatattg ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc
     4381 tttcccggag ttcctgttca gcaagcagac aaattgaccc aattacgtcg cttagcctgt
     4441 agtgtgtcgc aacgggataa taaaacagcc acatttgact tcagcgcctg ttctttagaa
     4501 tggcaaaaca ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac aacacagcta
     4561 ccatcaccag taatggctgt actcacggca cttgaaatga aatgcacaag atataaagtg
     4621 cgtgaggatg ttatggatca gatcgtccag gaaggcggtc tggaatatgc tactgatgta
     4681 ataatacacc ttcaacagat tgatattaaa tgggattatg cgaataatgt cattattatt
     4741 ctgccgtctg gcattgcacc tgactacttg gagcaatatt ccagatttga attacgccta
     4801 cgtaaacatt tatcactggc ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt
     4861 gccgcaattc cacatattcc agaatggcgg caaccattaa ttgctttgtt attacccgaa
     4921 aaaccagaaa ttgcacatga aattgcccag cgtctactgg ggcaaaaaaa attaccctcg
     4981 cttgagtggt taaaaatagt ggcgactgat gagcacattc ttgcctcatt agaaaaatat
     5041 cacgagccat atgccatttt tgatgattac tattgtggtg cgatatggtc agccaccgta
     5101 ttacaggagc aaggtgttgc agccctgccc cgatttgctc cctatgccgc aagtgactac
     5161 tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat taacactgct tatacgtgta
     5221 gccgggcata ctaaacgctg tcacgatcgg atgacgaaag cctgcgctgc gttcccacat
     5281 gcagcaatgg cggcactgac ggaacttctt gggcaaaaag aagagaacag ttggcgcatt
     5341 atgctaatga caatgcttat ctcacaacca gcactggcag aacaggtcat tccctggctc
     5401 tcgacacccg cagttgccgt actgaaatca tgccagcaac aactgacaca gccctcaaac
     5461 catgccagcg ccgatctact gccagccgta gtagtctccc ctccctggct ttcgaaaaag
     5521 aaaaaatcgc cgattccggt gctggattta gcgccattag gcattgagcc aatctgttat
     5581 ctgacagaag aaatcagtaa tcaacttttg gcgaaatata tctggtattc aaaacacatc
     5641 acggttagcc atgaagaaag tactaccaac ctgttggcaa ggatgggttt tcaacgacgg
     5701 atcgctggta catatattaa agctcccgaa gcggtagttg aggcatggct aaatgaagat
     5761 tattcaacct tactaagtga atttaaggtg tttcattcac ctaccgggca ttattggcag
     5821 ttggggattt tgacaacatt gccgctggag aaagcagtaa aagcatggaa tgcccttacc
     5881 ctatctccac ataccgatac cgaatacgcc atgttacatt ttggactcaa agggttacct
     5941 gggttagtaa actcacttgc acgctatcca caagaagcct tgcccatcac gaattacttc
     6001 gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca acaaactgaa aacgctacgc
     6061 gaaaacgccc gtagctggct gttgaaatac ccggaacatg cccttaccgg cctgctgcct
     6121 gcggcgctcg gcaaagccgg tgaagcacag gataacgccc gcgctgcctt gcgtatgctt
     6181 accgaaaacg gtcatcagcc attactgcaa gaaatcgccc gacgttataa ccagccggaa
     6241 gtaaccgatg cggtgaacgc tctgcttgcg ctcgatccct tagataatca cccgacaaaa
     6301 atccccactc ttccggcctt ttatcagcca tcgctctgga cgcgcccggt attaaaagca
     6361 aatgcccaat cactgccaga tagcgccctc ctccacctcg gtgaaatgct ccgcttccct
     6421 caggaagagg ctctgtatcc gggattattg caggtgaaag acgtctgttc cgccgactca
     6481 ctggcgggat ttgcctggga tctgtttacc gcctggcaga ccgctggcgc gccgtcgaaa
     6541 gagagttggg cgttcactgc gttaggcgtt ctcggtaacg atgacaccgc ccgcaaactg
     6601 acgccattaa tacgcgcctg gcctggtgaa tcccagcata aacgcgccac cgttgggttg
     6661 gatattctcg ctgctatcgg tagtgatatc gcccttatgc agcttaacgg catcgcccag
     6721 aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa aaattgccga cattgccgag
     6781 agccgcgaac tcacggtggc ggagcttgaa gatcggttag caccggatct cggtctggat
     6841 gataacggtt cgctgctgct ggattttggc ccacggcagt tcaccgtcag ctttgatgaa
     6901 accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc tgaaagacct gcccaaaccg
     6961 aacaaaagcg atgatgaatc gcggtcgaac gatgcggtta accgctacaa attgctgaaa
     7021 aaagatgcgc ataccgtcgc cgcccagcag gtagcaaggc tggaatccgc catgtgcctg
     7081 cgccgccgct ggtcgccgga aaacttccag ctcttcctgg ttgagcatcc gctggttcgc
     7141 cacttaaccc gccgtctgat ttggggcgtt tatagcgccg aaaaccagct actggcttgc
     7201 tttcgcgtag cagaagataa cagctacagc accgctgacg atgatctttt caccctgccg
     7261 gaaggcgata tctctatcgg cactcctcac gttctggaaa tatcaccaac ggatgctgcc
     7321 gcctttggtc agctttttgc cgactacgaa ctgctaccac cgttccgcca gctcgaccgt
     7381 aacagctacg ccctgacaga agccgagcgc aatgccagtg aactgacccg ctgggcaggc
     7441 agaaaatgcc cgagtggtcg ggtcatgggg ctggcgaata aaggctggat aaagggcgaa
     7501 ccacaggatg gaggctggat cggatggatg atcaaacctt tgggtcgctg gtcgttaatc
     7561 atggaaatcg acgaaggttt tgcggtgggt atgtcaccag ccgaatttag cgctgagcag
     7621 ctcttaagca agctgtggtt atgggaaggc aaagcggaaa gctatggctg ggggagtaat
     7681 tcaacacagg aagcgcagtt ctccgtactc gatgccatca ccgccagcga gctaattaac
     7741 gatattgaag ccctgtttga ataaggaaag agcatggaca aggaattacc gtggctggcg
     7801 gataacgccc aactggaact gaaatataaa aaaggcaaaa caccactcag tcatcgcaaa
     7861 tggccgggcg aaccggtgcc tgttatcact gaaagcatca ttcagacact gggtgatgaa
     7921 ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc gttatgaaaa tttttcactg
     7981 gagtggcagt ccgccatcac gcaggccatc aacttgatcg gcgaacacaa accctcaatc
     8041 ccggcccgga caatggcggc gctagcctgt atcgcgcaaa atgacagcca acagttgctc
     8101 gacgaaatcg tccaacaaga ggggctggaa tatgcgactg aggtggtgat tgcacgccag
     8161 tttattgcgc ggtgttatga gagtgatcct ctggtagtga cattgcagta tcaggacgag
     8221 gattatggct atggttatcg ctcagaaacc tataacgaat tcgatctccg actgcgtaag
     8281 catctctctc tggcagagga aagctgctgg cagcgttgcg ccgacaaact cattgccgca
     8341 ctaccaggaa taaccaaagt tcgccgccct tttattgcgc tgatcctccc ggaaaaacca
     8401 gaaatagcca atgagttggt aggccttgaa tgcccgcgaa ctcattttca ttctaaggag
     8461 tggttaaaag ttgttgctaa tgaccccaca gcggtgagaa aactcgagca ctactggagc
     8521 caggatatat ttagcgatcg agaagccagc tacatgtcgc atgaaaacca cttcggctac
     8581 gcggcctgcg ccgccctttt gcgcgaacaa ggactggcag ccattccgcg cctcgcgatg
     8641 tatgcccata aagaagattg cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc
     8701 cgcaccttgc tactggtggc tgataaaaac aaacccagcc tgcaacgtgt agctaaatac
     8761 cataaaaact tcccccatgc gacgctcgcc gcactggcag aactgctggc gttaacagaa
     8821 ccaccagccc gcccaggtta tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa
     8881 gcacgcgatg aatactggcg tacgctgtta caaacgctga tggcatcgca gccacaactg
     8941 gcaggagaga tgatgcagtg gttaagtact caagccaggg cagtgctgaa tagttattta
     9001 tcggcaccgc ccaaaccggt tattgatagt accgataaca gcaatctgcc tgaaatcctc
     9061 gtttcaccac cgtggcgtag taagaaaaaa atgacagctc cacgtcttga tttggcaccg
     9121 ctcgaattaa cgccgcaagt ttactggcaa cctggcgaac gagagaggct tgccgccact
     9181 gagcctgcct gttatttcag cacggaatct cttgcggaac gcatggaaca aaaaagtggg
     9241 cgagttgtat tacaggaact gggttttggg gatgatgtat ggctgttttt gaattatata
     9301 ctccccggaa aactggatgc tgcacgcaat tcactcattg ttcagtggca ttactaccag
     9361 gggcgggttg aagagatcct gaatggctgg aactccccgg aagcacaatt agcagaacag
     9421 gcgctccgca gcggtcacat agaagcgtta attaacatat gggaaaatga caactactca
     9481 cgttatcgtc cggaaaagag tgtctggaac ctgtatttat tggcacagtt gccgcgtgag
     9541 atggctttga ccttctggct gcgtatcaat gagaaaaaac atctgttcgc gggtgaggac
     9601 tattttctca gtatcctcgg gttggatgcg ctaccaggtc tgatgttggc tttttcacat
     9661 cgtccaaaag aaacatttcc gttaatttta aatttcggcg caacagaact ggccctgccc
     9721 attgcccgcg tctggcaccg ttttgcgggc cagcgtaatc tggctcgcca gtggatttta
     9781 caatggccgg aacatacggc tactgcactt atccctctta tcttcaccaa atctagcgat
     9841 aaaagcgaag ctgcattact tgccctgcgt ttactttacg aacatgggca tggcgaattg
     9901 ctacaaactg tagccaatcg ttggcagcgt acagatctat ggcctgccct ggaacaatta
     9961 cttaaacaga gtccaataga gatttacccg gcacgcattc caaaagcccc tgatttctgg
    10021 catccgcaaa tgtggtccag gccgcgcctt atcactaata atcaacctgt taccgatgac
    10081 gctctggaaa ttatcggcga aatgctgcgc tttacccagg ggggacgttt ttatagcggg
    10141 ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc ctgggatctc
    10201 ttcactgcgt ggcaacaagc tggtgccccc gtaaaagaca actgggcatt tctggcgtta
    10261 agtctctttg gtgacgaaag cacggcacgg gatctaacga cacagatcct cggctggcca
    10321 caagaaggca aatctgcccg tgctgtcagt ggcctgaaca tccttaccct gatgaataat
    10381 gatatggcgc tgatacagct gcatcatata tcgcaacggg ctaaatcccg ccccttacgt
    10441 gataacgcgg cggaatttct tcaggtggtc gcagaaaatc gcgggctaag ccaggaagag
    10501 ctagcggaca gattagtccc aaccctgggc cttgatgatc cgcaggcgtt gagttttgat
    10561 tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc tcaacccggt tatctttgat
    10621 cagcaaaacg ttcgccagaa aagcgttccc cggttgcgcg ccgatgacga tcaactgaaa
    10681 gcgcccgagg cactggcccg actaaaaggg ctaaaaaaag atgctactca ggtgagcaaa
    10741 aacctgctcc cgcgtcttga agctgcccta cgtaccaccc gacgctggtc gctggcagat
    10801 tttcatactc tgtttgttaa tcatcccttt acccgcctgg ttacccagcg attaatatgg
    10861 ggcgtgtatc cggcaaatga accgcgtcgt ttactcaacg cctttcgtgt ggccgcagag
    10921 ggggagttct gcaatgcgca ggatgagcca attgacctgc ctgcggatgc tctgattggc
    10981 attgcccacc cgttagaaat gacagcagaa atgcgcagtg aatttgccca gctttttgcc
    11041 gattacgaaa ttataccgcc acttcgccag ttgacacggc gcacggtgct gctcacgcct
    11101 gacgagtcag ccagtaatag tttgaatcgc tgggaaggta aatctgctac cgttgggcaa
    11161 cttatgggaa tgcgatacaa aggctgggag tcaggctatg aggacgcatt tgtctatgac
    11221 ctgggtgagt accggctggt ccttaagttt tcacccggtt ttaaccacta caatgttgat
    11281 agcaaagcgc taatgagctt ccgttctctt cgagtgtaca gtgacaataa atccgtcact
    11341 tttgccgaac ttgatgtgtt tgatttgagt gaggcgttaa gcgcacctga cgtcattttc
    11401 cattaaaaca cagcgggcag tgatgcaact gcccgttatc aacacgactt accagcgggg
    11461 aaagcgatga tcgtgcaaaa agagctggtt gctatttacg attatgaggt ccctgtacct
    11521 gaagatccgt tttccttcag acttgagatc cataaatgct ctgaattatt tacaggttcc
    11581 gtctatcgac tggagcgatt ccggctacgt ccaacatttc atcaacgtga tcgagaagat
    11641 gctgacccgc taataaatga tgcgttgatt tatataagag atgagtgtat tgatgagcgg
    11701 aaattacgag gtgaatcacc tgaaactgt
//
LOCUS       DAPPNJ010000141        11692 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000141 DAPPNJ010000000
VERSION     DAPPNJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.142"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(59..643)
                     /gene="pth"
                     /locus_tag="RPM73_003212"
     CDS             complement(59..643)
                     /gene="pth"
                     /locus_tag="RPM73_003212"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDX6521546.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            921..1199
                     /gene="ychH"
                     /locus_tag="RPM73_003213"
     CDS             921..1199
                     /gene="ychH"
                     /locus_tag="RPM73_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDX6521547.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1254..2933)
                     /gene="dauA"
                     /locus_tag="RPM73_003214"
     CDS             complement(1254..2933)
                     /gene="dauA"
                     /locus_tag="RPM73_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDX6521548.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3058..4005)
                     /gene="prs"
                     /locus_tag="RPM73_003215"
     CDS             complement(3058..4005)
                     /gene="prs"
                     /locus_tag="RPM73_003215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDX6521549.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4156..5007)
                     /gene="ispE"
                     /locus_tag="RPM73_003216"
     CDS             complement(4156..5007)
                     /gene="ispE"
                     /locus_tag="RPM73_003216"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDX6521550.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5007..5630)
                     /gene="lolB"
                     /locus_tag="RPM73_003217"
     CDS             complement(5007..5630)
                     /gene="lolB"
                     /locus_tag="RPM73_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDX6521551.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            5844..7100
                     /gene="hemA"
                     /locus_tag="RPM73_003218"
     CDS             5844..7100
                     /gene="hemA"
                     /locus_tag="RPM73_003218"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDX6521552.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7142..8224
                     /gene="prfA"
                     /locus_tag="RPM73_003219"
     CDS             7142..8224
                     /gene="prfA"
                     /locus_tag="RPM73_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDX6521553.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8224..9057
                     /gene="prmC"
                     /locus_tag="RPM73_003220"
     CDS             8224..9057
                     /gene="prmC"
                     /locus_tag="RPM73_003220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDX6521554.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9054..9446
                     /locus_tag="RPM73_003221"
     CDS             9054..9446
                     /locus_tag="RPM73_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDX6521555.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9450..10259
                     /locus_tag="RPM73_003222"
     CDS             9450..10259
                     /locus_tag="RPM73_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDX6521556.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            10295..11149
                     /gene="kdsA"
                     /locus_tag="RPM73_003223"
     CDS             10295..11149
                     /gene="kdsA"
                     /locus_tag="RPM73_003223"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDX6521557.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(11298..11405)
                     /locus_tag="RPM73_003224"
     CDS             complement(11298..11405)
                     /locus_tag="RPM73_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX6521558.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         2916 a         2985 c         3066 g         2725 t
ORIGIN      
        1 atggaaataa ctttgcctat tatacacggc actcggcaaa aatgccgcag acaacgactt
       61 attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt caagccatct gtaaaccaca
      121 tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat taacttctgt tcactaacag
      181 gcggtttgcc taacacaaaa ccgacaactt tatttttatc gcccggatga ccgattccga
      241 tgcgtaaacg gtgaaagtta gggttattac ccaatttact gatgatgtct ttcagtccat
      301 tgtgaccacc atggccaccg cccaatttaa atttggcgac gccaggaggc agatccagtt
      361 cgtcgtgggc caccagaatt tcgtccggat taatgcggaa aaaactggcc atcgccgcaa
      421 cggctttgcc gctgagattc ataaatgtag tcgggactaa caggcggaca tcttcgcctc
      481 caagagtgac tcgcgaagta taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca
      541 aacgctctgc cagtaagtca acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt
      601 cagcaccggg gttcgccagg ccgacaatca atttaatcgt cacgtttttt tgtcctgagt
      661 gtgtacataa ctggcgcgta gtttactggt tgcggccccg cttgacaaaa aactgcgtat
      721 caaatgcaga taacgtaata attgcctgag tggactatta gaaagtcaag gtgttcaggc
      781 gtttatttgt aaagttttgt tgaaataagg gttgtaattg tgatcacgcc cgcacataac
      841 ccacagggtg ttgtctatac tttacacata aggaagaggg gtattccctg ttacaaccca
      901 gaaagttccg gaggtgacat atgaaacgca aaaacgcttc gttactcggt aacgtgctca
      961 tggggttggg tctggtggta atggtggtcg gcgtggggta ttcaatcctc aaccagttac
     1021 cacagtttaa tatgccccag tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg
     1081 ccattctctg gctggcgggt gcccgtgttg gcgggcatga acaggtgtgc gaccgttact
     1141 ggtgggttcg ccactatgac aaacgttgcc gccgtagcga taatcgccgt catagctaac
     1201 aaataatgca gtttgctgac cagtcagttt gcgctgactg gtcaatctcg tacttataaa
     1261 tccgccatcg ccgcgcgacg attcgggaag aacgccaggc gtcccgggat cggttgaatg
     1321 ccagcgcgag ccatagtgcg cagtggctgg aattccacgt tgcacacgcg cagttcacat
     1381 ccctcgggca gacgcttcac aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc
     1441 ggaaccgcat cccacttcag aatcacaatc cgtttgcctt caagacgtga ctccaggtcc
     1501 gtgaacaagc cttcagcagc agcaaaaaac agcgggccaa taacgcgcag taccagaaca
     1561 tcgtctggaa catctacgac taccggtgcc aggcgagtca tacgtgcgat acgacgcata
     1621 aacagcagcg atgccagcac gatccccacg ctgatggcaa taaccatatc aaacagcacg
     1681 gtcagcgaca tgcacagcag catgacaatg atgtcatctt tcggcgcatg acgcagcaag
     1741 tcgaccactt tgtgcgcttc actcatgttc cacgccacca tcaacagcag ggcagccatg
     1801 gcggaaagcg gcagccagga gagcagcggt gccagtacca gcagggcaag aataaccaga
     1861 atagagtgga tcaccgccga gataggggaa gttgccccgg cacggacgtt agcggcagaa
     1921 cgcgcgatgg cagctgtagc ggtaatgcca ccaaagaacg gagcgatgat attccccagt
     1981 ccctgtccaa ccagttcgct attcgccttg tgtttcgtcc cggtcatacc atccagcacc
     2041 acggcgcaga gcagagattc gattgcgccg agcattgcca ttgagaatgc cgcaggcagc
     2101 agtgtgcgaa tagaatccca ggttagcgtg aattctgaat taggcagatc ccacggcagc
     2161 accagttgcg gcagcagttg cggaataccg ttaccctgag aaccatcggc caggacgtag
     2221 tggaattgcg aaccgatggt agcaacatgt ccgccgagca ggttaacaat ccccatcacc
     2281 gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac gaatgcccag acgcggccaa
     2341 aaaacaagaa tacctagcgt cacaatgcca atggcagcat cacccacatt aatggtcggc
     2401 agcgccataa ataatgcgcc gactttttgt agataatgtt ccgggacatg ggccatttgc
     2461 agaccgagaa aatctttaat ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa
     2521 cctaaggtga cggaaaccgg aatatactca atcaggcgac caaagcgtgc cagacccatc
     2581 agaatcaaaa aaatccccga cagcaaggtc gcaaccagca gtcctgccag tccaaactgt
     2641 tgcgacacgg gatagagaat taccacaaat gccgcagtcg gaccggaaac gctaaagcgt
     2701 gacccacccg tcagagcaat gacaatcccc gcaacagctg cggtatataa accgtactgg
     2761 ggtgccacac cactaccaat agccaacgcc atcgccagcg ggatagcaat aatcccgacg
     2821 gttatcccgg caatcaggtc acgggtaaac cgtgcggcag tatatttttc tttccagcaa
     2881 gcgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa atattttgtt cacaataatg
     2941 tttcatccgt gagcgcatca tctgtcaact aaatggcagg tgaaggaggc ataggtcata
     3001 caaatggata ttacagacaa aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta
     3061 gtgttcgaac atggcagaga tcgattcttc gttgctgata cgacgaatcg cttcggccag
     3121 catacctgac agggtcagag tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg
     3181 aatggtatcg cagacaacga cttcatcaat tacagagtta cgcaggttat tcgccgcgtt
     3241 gccagagaag atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac gttctttcag
     3301 agcttcagca gctttacaca gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca
     3361 gtcacgacct gcaacgtcac cgatgatatg catcacctgg gaaacgttcg cacgcggacg
     3421 acgtttgtcg atgattgcca tatcggtatc gttcagcagc ttagcgatag cgcgggcacg
     3481 cacaacgccg ccgatgtccg gagaaaccac aattgggtta tccagattca gctgcagcat
     3541 gtcttccagc aggatcgggc taccaaatac gttatcaacc ggaacgtcga agaaaccctg
     3601 aatctgttca gcgtgcagat ccactgtcag cacacggtca acaccgacgc tggagaggaa
     3661 gtctgcaacc actttcgcag tgattggtac acgagcggaa cggacgcgac ggtcctggcg
     3721 cgcatagcca aagtagggga taacagcggt gatacgacct gcggaagcac gacgcagggc
     3781 atcaaccata acgactaatt ccatcaggtt gtcgttagta ggggcacaag tggactggat
     3841 gatgaaaata tcaccaccgc gtacattttc attaatttgt acgctgactt cgccatcgct
     3901 aaagcgacct acagcggcgt cgccgagtga agtgtacagg cggttggcaa tacgttgtgc
     3961 tagttccggg gtggcgttac cagcaaaaag cttcatatca ggcacgagaa gaacctcagg
     4021 catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc gggccaggca tgatcctttc
     4081 caggcggtgt attaaagagc gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa
     4141 ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg gaaagattaa cgcctttcgc
     4201 cacaaagcca ttgagccatt ccggggcttg ctctagcacc tggcgggctt cagactctgt
     4261 atcaaattca gcaaagacac aggcccctgt cccagtcagg cgcgacgggg cgtattctaa
     4321 cagccaggaa agcaccgcat caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc
     4381 attgctgaat tcacatttta gcaacgtttc tattgacctt tttggcgtat tgcgcgggag
     4441 ttcaggatct ttaaaaatca ccggagtcgg aatacttaca ccagggtgcg ccaccagata
     4501 ccacttctct ggcggatcca ccggcgttag tatttcacca acgccttcgg caaacgcggc
     4561 atgcccccga acaaagacag gaacatctgc gcccagcgtc agccccattt ccgccagctc
     4621 atccatgctt agcccgcatt gccagagatg atttaatgcc accaggaccg tcgcggcatt
     4681 ggatgaacca ccgccgagac cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc
     4741 accgcttccc gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc
     4801 gcgaacgatc aggttatctt catgttccac gccttcaacg ggcgttaaca gacgaatatc
     4861 cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt
     4921 ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg tataaaaaca gattaagttt
     4981 tgccggagag ggccactgtg tccgcattat ttcactatcc agttatccat ttttaacttg
     5041 atgcgttgac caccgtcggt gagttccata ttggctggca tcgcaggttg cgttttggtg
     5101 tcataaccac cataaacaac cttccagttt ttgccattct ggctgtaggt aatttcgctc
     5161 aggcggtact ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc taaaatccac
     5221 tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt tgccaatcat ctcttcggcg
     5281 tcatcggcgg tataacgctg acctttattg tcgactaact gcacgttacc cggttgagca
     5341 ttcagctcca gttccgtgct gcccaatggg ttagtgagca gcagacggta gcgatcctgg
     5401 ccggtttgct gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat ataagcgaac
     5461 gcgccgcgag tctgatactg attaagattg cgcacgtctt gctgatgctg acgccattgt
     5521 ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc agtaagcaca
     5581 agagcagcca gcggtagcag gcggataaga cgaaaatcgg gcaggggcat agtgatgaca
     5641 agtccttgag atacgttgca gttataaccc ttaatgctag cgttaccgtc cgctatcgtc
     5701 tatgttcaag ttgtcttaat tgccagaatc taacggcttt cggcaattac tccaaaaggg
     5761 ggcgctctct tttattgatc ttacgcatcc tgtatgatgc aagcagacta accctatcaa
     5821 cgttggtatt atttcccgca gacatgaccc ttttagcact cggtatcaac cataaaacgg
     5881 cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat caggcgcttg
     5941 acagcctgct tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca
     6001 cggaacttta tcttagcgtt gaagagcagg ataacctgca agaggcgtta atccgctggc
     6061 tttgcgatta tcacaatctt aatgaagaag atctgcgtaa aagcctctac tggcatcagg
     6121 ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg cctggattca ctggttcttg
     6181 gggagccgca gatcctcggt caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata
     6241 tgaaggccag cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc
     6301 gcactgaaac agatatcggt gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg
     6361 cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa
     6421 ccatcgagct ggtagcgcgt catctgcgcg aacataaagt acagaagatg attatcgcca
     6481 accgcactcg cgaacgtgcc caaattctgg cagatgaagt tggcgcggaa gtgattgccc
     6541 tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc accgccagcc
     6601 cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt aaaaagccgt cgcaaccaac
     6661 caatgctgtt ggtggatatt gccgttccgc gcgatgttga gccggaagtt ggcaaactgg
     6721 cgaatgctta tctttatagt gtggacgatc tgcaaagcat catttcgcac aacctggcgc
     6781 agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta
     6841 tggcgtggct gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc agccaggcag
     6901 agcacgttcg cgatgagtta accgccaaag cgttagcggc ccttgagcag ggcggcgacg
     6961 cgcaagccat tatgcaggat ctggcatgga aactgactaa ccgcttgatc catgcgccaa
     7021 cgaaatcact tcaacaggcc gcccgtgacg gggataacga acgcctgaat attctgcgcg
     7081 acagcctcgg gctggagtag cagtacatca ttttcttttt ttacagggtg catttacgcc
     7141 tatgaagcct tctatcgttg ccaaactgga agccctgcat gaacgccatg aagaagttca
     7201 ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg cattatcacg
     7261 cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc gactggcaac aggttcagga
     7321 agatatcgaa accgcacaga tgatgctcga tgatcctgaa atgcgtgaga tggcgcagga
     7381 tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa cagcaattac aggttctgtt
     7441 actgccaaaa gatcctgatg acgaacgtaa cgccttcctc gaagtccgag ccggaaccgg
     7501 cggcgacgaa gcggcgctgt tcgcaggcga tctgttccgt atgtacagcc gttacgccga
     7561 agcccgccgc tggcgggtag aaatcatgag cgccagcgag ggtgaacatg gtggttataa
     7621 agagatcatc gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat ttgaatctgg
     7681 cggtcatcgc gtgcagcgtg ttcctgctac ggaatcgcag ggtcgtattc atacttctgc
     7741 ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca tcaacccagc
     7801 agatttacgc attgatactt tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac
     7861 cgattcggca attcgtatta ctcacttgcc gaccgggatt gttgttgaat gtcaggatga
     7921 acgttcacaa cataaaaaca aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc
     7981 tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc cgtcgtaacc tgctggggag
     8041 tggcgatcgc agcgaccgta accgtactta caatttcccg caggggcgcg ttaccgatca
     8101 ccgcatcaac ctgacgctct accgcctgga tgaagtgatg gaaggtaagc tggatatgct
     8161 gattgaaccg attatccagg aacatcaggc cgaccaactg gcggcgttgt ccgagcagga
     8221 ataatggaat atcaacactg gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc
     8281 ccgcggcgtg atgctgaaat cctgctggag catgttaccg gcagagggcg tacttttatt
     8341 ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc agcaacttga tgcgctactg
     8401 acacgtcgtc gcgatggtga acccattgct catttaaccg gggtgcgaga attctggtcg
     8461 ttgccgttat ttgtttcgcc agcgacctta attccgcgcc cggatacgga gtgtctggtg
     8521 gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta ttcttgatct cgggacgggt
     8581 accggggcga ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat tatcgctgta
     8641 gatcgtatgc ctgatgctgt ctccctggca caacgtaatg cccagcatct ggcgatcaaa
     8701 aatatccaca ttctgcaaag cgactggttt agcgcgctag ccgggcagca gtttgcgatg
     8761 attgtcagca atccgccgta tattgacgag caggacccac atcttcaaca aggcgatgtc
     8821 cgctttgagc cgctcactgc gctggttgcg gcagacagtg gaatggcaga catcgtgcat
     8881 atcatcgaac agtcgcgtaa cgcgctggta tccggcggct ttctgcttct ggaacatggc
     8941 tggcagcagg gcgaagcggt gcgacaagca tttatcctcg cggggtatca tgacgtcgaa
     9001 acctgccgtg actatggtga taacgagcgc gtaacgctcg gccgctatta tcaatgacaa
     9061 gtttttctac actgcttagt gttcatctta ttagtctcgc gctttctgtt gggctattaa
     9121 ccttacgttt ctggctacgt tatcagaagc atccacaggc atttgctcgc tggacgcgca
     9181 ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg cattgcgttg atggctaaag
     9241 cgcacatcct gccattttcc gggcaggcac agtggctgac tgaaaagctg tttggagtta
     9301 tcatttatat cgttttgggt tttattgcac tcgattatcg tcgtatgcac agtcagcagg
     9361 cgcgcattat tgccttcccg ctggcgttgg tggtgctgta catcatcatt aaactcgcca
     9421 ccacaaaagt accgttactg gggtaagtca tgagatcgtt agctgatttc gaatttaata
     9481 aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc gattttccct
     9541 cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc
     9601 agcttctgcc tttagaagag cagctggaaa aactgatcgc gctgttttac ggcgactggg
     9661 gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc attatggctg gaccaggtgt
     9721 taaagaatcg acagggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
     9781 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
     9841 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
     9901 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
     9961 ttgatgaagc tgataacatt gaggtaatcc gcaaattgct ggatacactc aaagcctcgt
    10021 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    10081 accctgaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    10141 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    10201 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    10261 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    10321 cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggcggtatga acgtgctgga
    10381 atctcgcgat ctggcgatgc gcatttgcga gcactacgta actgtgaccc agaaactggg
    10441 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    10501 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    10561 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    10621 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    10681 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    10741 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg
    10801 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    10861 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    10921 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    10981 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    11041 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    11101 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    11161 tgaaaaataa agtcttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    11221 aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
    11281 tgacgcccgt tttcacctta cttccggtta cgccaccagc cgacaatcgc tgcggtaata
    11341 attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc
    11401 gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa ttgttctggt
    11461 tcaagattag cccccgttct gttgtcaggt taaacctctc aacgtgcggg ggttttctct
    11521 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
    11581 gccggcttga tgtggatact acgtctcaat tcatcttcac ttcatccctg aaatgtttgc
    11641 aatgaagagt gcattccggt ttttgaacag ctgttacagt cgtttcatga gg
//
LOCUS       DAPPNJ010000142        11601 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000142 DAPPNJ010000000
VERSION     DAPPNJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.143"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1389)
                     /gene="cadC"
                     /locus_tag="RPM73_003225"
     CDS             complement(<1..1389)
                     /gene="cadC"
                     /locus_tag="RPM73_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDX6521559.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMK"
     gene            complement(2271..2597)
                     /locus_tag="RPM73_003226"
     CDS             complement(2271..2597)
                     /locus_tag="RPM73_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX6521560.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(2594..2857)
                     /locus_tag="RPM73_003227"
     CDS             complement(2594..2857)
                     /locus_tag="RPM73_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDX6521561.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(2929..3795)
                     /locus_tag="RPM73_003228"
     CDS             complement(2929..3795)
                     /locus_tag="RPM73_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDX6521562.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            complement(3880..4077)
                     /locus_tag="RPM73_003229"
     CDS             complement(3880..4077)
                     /locus_tag="RPM73_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521563.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(4089..4577)
                     /locus_tag="RPM73_003230"
     CDS             complement(4089..4577)
                     /locus_tag="RPM73_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521564.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(4574..4951)
                     /locus_tag="RPM73_003231"
     CDS             complement(4574..4951)
                     /locus_tag="RPM73_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDX6521565.1"
                     /translation="MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(5041..5409)
                     /locus_tag="RPM73_003232"
     CDS             complement(5041..5409)
                     /locus_tag="RPM73_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDX6521566.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKRLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(5489..5710)
                     /locus_tag="RPM73_003233"
     CDS             complement(5489..5710)
                     /locus_tag="RPM73_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX6521567.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(5797..6273)
                     /locus_tag="RPM73_003234"
     CDS             complement(5797..6273)
                     /locus_tag="RPM73_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDX6521568.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(6288..6773)
                     /locus_tag="RPM73_003235"
     CDS             complement(6288..6773)
                     /locus_tag="RPM73_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX6521569.1"
                     /translation="MTTSSHNSTTPSVSVTTASGNNQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
     gene            complement(6865..7683)
                     /locus_tag="RPM73_003236"
     CDS             complement(6865..7683)
                     /locus_tag="RPM73_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDX6521570.1"
                     /translation="MQLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDKHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(7773..7982)
                     /locus_tag="RPM73_003237"
     CDS             complement(7773..7982)
                     /locus_tag="RPM73_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX6521571.1"
                     /translation="MPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIRNAEGQLRW
                     RCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(8012..8689)
                     /locus_tag="RPM73_003238"
     CDS             complement(8012..8689)
                     /locus_tag="RPM73_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521572.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(8837..9517)
                     /locus_tag="RPM73_003239"
     CDS             complement(8837..9517)
                     /locus_tag="RPM73_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDX6521573.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(9720..10604)
                     /locus_tag="RPM73_003240"
     CDS             complement(9720..10604)
                     /locus_tag="RPM73_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDX6521574.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSHICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPEVDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQALSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            10789..>11601
                     /locus_tag="RPM73_003241"
     CDS             10789..>11601
                     /locus_tag="RPM73_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521575.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLVELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYPANSSGVSGYSDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPIFGLLFYEQERLTGGVFRY"
BASE COUNT         2963 a         2658 c         2916 g         3064 t
ORIGIN      
        1 cttcatttca taaaccttgc caagcaacac ataatttagc caggacattt caagatcaat
       61 gccagtattt atcgcctggt aagactcatc tgttttacct tttaccagag cactgaccgc
      121 ttttatttga tatataatgg ataggttatt caattccggt agtgtaacaa tgttgtctat
      181 ttctgtgttc agtgctgcta attgtttttc atctaaaggg tgttgagaat ggcgcacgat
      241 atcaactaat gctttttctg ctctcgcgta ggtaaattct ggggatgatt gaacaatctc
      301 acctaataat tcactggcac ggttcaatga tttatcatcg ccatgcagta aataatcatg
      361 tgcctgataa aaattagtta ataacgcacc acgatgaggc aaaatttgct ggagcgtctc
      421 ctgcattcgt tgtggccacg gttggtttaa tgcttttgat aaactctcca gtaaatcatt
      481 ttgaatcgcc agctgattac cgttagtgat gacataacgt ttatccagca tggttgaacc
      541 atctgcattg tctaccaatt ttatcgacat aaagcattgt tgagcacggt attggcgctg
      601 attaacaaag gcaatagata atgttttacc ggaactgctc ggttcatcaa tgttgtagtt
      661 gattttgtca tgcaccataa aggtggagaa ggtgttaagt gatgtcgcca ccaaatcacc
      721 cacgcctatc gcgtaagaga gctgatacgg ggaactccag ctgttacaac ttttatttac
      781 catattaatg tcaatatcgc gtggattgag caaaatacgc gatttgctca taggaagacg
      841 tgtatcaaga gttgaaaacg ctaccagtgc tacacagata cctaacgaca acaggaaaaa
      901 aaaccatacc caaaaggtag tgaatcgttt gcttttaact ggggattgtt caggtggcgt
      961 tgcggtgttt tgaatgttaa gactgtggga gggagaatct gtggcaggaa ccgcctctgg
     1021 tataggggga ggcgaagata gcattatttc ctctccctct tcttcgctgt accagataac
     1081 cggcaccatt aatttatagc cgcgctttgg tacagtagcg atatagacag gactatcttc
     1141 atcattatct tttaatgact tacgtagttc tgagatactc tgcgtcacaa catgattggt
     1201 gacaatactt ctcttccaga cattatcgat aagttcatcc ctgctaagta cttcgccact
     1261 gtgttgagca aagaaaacca gaagatcgat taatctcggc tcaagggtaa gttgacgccc
     1321 attgcggcta atttggttta tggacggagt aacaagccat tcgccaacgc gaactacagg
     1381 ttgttgcata atagaaactc attcgaaaag ggaatgatgc aatgataatt gccacaacct
     1441 atttttacca tctatagatg ggtttattta catattattg gtgaatgcaa gacgttattt
     1501 ttaccagcca taaacttctt gattacatag tattacgaaa ggattttact gagaaccaga
     1561 agtaatattc cttaccatca aaattcatca tctttgccaa agaaaaatgt tcagaaaata
     1621 atccatggaa aattgtccgg agcgcttact attttaatgg attgttagtc tttgcatgag
     1681 caagcggact gatacatttc tctttgttct cattcagaaa atctcatcag tcggcgttca
     1741 gaccggtgtt gtgctttatg gatgcgttac gctcctgatg acgtcatttg acgttcaaca
     1801 acatcacggg gccgcacgac atttcacgtc agttagtgct atcgctcagg aacaaatttt
     1861 cccgaattgg gatatgcccg caaattgctg gtgatgtggg gagaatctgg ttgagttcgg
     1921 gagaattgat ttggaggcag aacgcttaaa tcgtggcgtc ctgaaacgaa aaacggacct
     1981 ccgtggaggt ccgtttatat gaatttggtg ccggactcgg aatgaaactg gttatataac
     2041 caattgtttt gtatcgactg ttttatcctt gttctttttg gggccaccaa tgaggccccc
     2101 atgtgcaatt cgaattcttt tgacgctata atccctcttt tttgtgcttt gttgtaatca
     2161 ttttgtctgg ttcgatatgc tgaatcgcgg aatcgaagta acaacaaaat acagagattt
     2221 ataaaatacc tggctacctg tgttagggag cgttaacgca cagattttgt ttatggctgc
     2281 cagaaatgat ctgatggttt gtcgtctggc tctgcaagta ccctgttgat atcccgggaa
     2341 ccatgaacga tacgttcgat acgaacttca gtatccagta ccctgaatag cagaaggtaa
     2401 cgaccatacg aacaacctct gatccctgcc cctaactcgg gtctttctct gtagagtact
     2461 ggcgattcgg ctatcaacag acattgttgg taaagctctt cggtaaaact gattgcacgg
     2521 acaggattat cctgagcgat ccagtcacct atagcttcaa gatcgtgttc tgctaatggt
     2581 gaaacagtca gtttcatatt ggctgttcac cttctgccat ctttttgtat ttgtgcgtca
     2641 gtcgcccgaa tacctcttca gcacttttac tttcaccgct gttgatgcct gcggtaacgg
     2701 cgctctggag tgattcaagc tttatctgct gttcccgttc ttccagagca cgaagtccgg
     2761 cacgaatgac ttcacttgta ttgttatacc gaccactttc tatttgttcg cggataaacg
     2821 tttcaaagta aggactgagg gatacactgg taggcatggt gattttctcg ccgattgatt
     2881 actatcaatt aataatagtt aatattcgcg aattgtaaaa tacctctttt atgccttgcg
     2941 gcttttgttc actgtgcttg ccagggcttc cgagtagtgt ctggcaataa tatcattaag
     3001 cctgtcaacc agttctggtt tcctgaacgt gatgtgcgcg gagcccttct taaagtatct
     3061 gatgctgaac atctcatctt catagtcctc tttaccctga acagactgga tgtggtcatc
     3121 cagatgaatg gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatacag
     3181 cattctttcc aggtcggcaa gtcgatctgc ctgttgcccg gtgataagat gaaaccccca
     3241 ccggtcccac ctcaccaggt tgttgacgat aattttactg ccaaatttac agggtgaatt
     3301 gttttcgtaa ttccagctca gtcccctgaa aacattgatc accccacgct caaacacttc
     3361 atctttgttc tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcac tgatttccgg
     3421 aaaattatcg tattccagtg accggtacca tgtatcacgg gcctgcgcat ccattaatga
     3481 cagcatcccg gaacgttgca tcaggtcacg ccagatttcg cgatcgagat tgcgggtgat
     3541 ggctttcatt gctgtttccg gtttatccat cagccagcag ccacagcgaa aatcctgttt
     3601 catggcccag tcccgggcgg ttttacctcc aatgctttcc gtcagaactg agatttcagt
     3661 cagttgccgg ataagtgtgt cgatttgcag cagtgtactc tttcgtacag ccagaatgtg
     3721 gcggatactg gttgagcaga taacgtccgt atgcccgttg accacttccg gacgggaggc
     3781 tgtgatatca gacataatcg atattccttt ttgcagataa atgaaaacgc cagccctgtt
     3841 caggctggcg tcagtggaat gaagtctggt gagtaatatt taccggttct ggtgcagaag
     3901 ctgcagatga cgaagagtac ggacatcctc ccgggcccgt tcgatacagg gcaacagtgc
     3961 tgccgaatcc gttttatcct catcgttgtc aaagataatt cccgattcgc agtcgatatt
     4021 gtcctgcagc cacacaatca gaatatccag tgctgtttcc gtggttaatg atttcatgtt
     4081 gttcctggtt accagtcgaa agtgggcagg tcaggcaggc attcagcgct ggcatccaga
     4141 tgaatgagac tgacgccata acgccggata agtgtgacaa ccagacggcg gaacgttttt
     4201 gacagtccca ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt acgtaacaga
     4261 tatccgctac ctgtaaaatg aatccattct gattcgccga agtcattcgt ctgatgtgac
     4321 agcgagtaca accaggcatt gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg
     4381 gtaagggccg cagatggtgg cgtgcgctct gctgatgccc ggtcagattc atcaacaaca
     4441 cgaagggagt aaccgttatc gcagaccacg ttaatcagtt cagcgagagt aacaccatcg
     4501 acgtcaacga caatgcgccc catgttcagt gcctgcacgt taatgctgtc ggcttccagc
     4561 gtcagggcca gtttcattgt ttcgcccccg gatgtttacc cagggtaatg ttatttaccg
     4621 ttctgtaatt gtcgcgggcc atcaggccgg ttgcccggcg ggcccggagg atatcaatac
     4681 tgtttattaa ctgagaacgg gtacaggcgc tgaatcccgg ctggtcggta cgtaccagtg
     4741 cgtatttttc aacgagaaag ttcaccgcat cgcacagtga aatgcctgcc tcaatatgct
     4801 gctcaatcac acgttcatca gcgaacggtg tgtcattcag tgtgaggccg taatgctggt
     4861 ccagcagtcg ggtgagcagt gtctgccaga tggtgacggg agactggcaa tgcgatgcct
     4921 cccgtacgtg tgtatcgggt aatgtgttca tactgaagat tttcctgata tgcagagatg
     4981 aaaaatggaa aagtggcgtg gtgaaagcac caggccggag aagaaggtta ttctggagag
     5041 ttactttttc gtttcgggcg tcggataaac agccatataa acgtagccac aactgccgag
     5101 ggtgtcggct tcgcaggtca gcctttttgc atacagcgtg acggtatgct gatggcgggg
     5161 attcagttca ccgctggtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     5221 gtccagatgg tacgcatccg cgtcgctgaa ccggcctctg ataccggcgc ggtctgcaag
     5281 gtaatgcaac cggttaccct cctgcaccag acgtgcccca aaacagggcg tcacggtgca
     5341 gggcagtccc caccaggtgc ggtcgttatt gtcgtcggga agcgttgtcc cggggagtgt
     5401 gtctgacacg ataaaatcct cacagaaaat tggttaagaa tggtccggtg gtggcgataa
     5461 ttctgctctt ctgaatgccc ttccagactc agttcagggt gacgctcatc agccggacat
     5521 acggaccaaa actgtcctta cggcgttctg caaacacagc cagcacaccg ggaatatcct
     5581 gtacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     5641 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     5701 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     5761 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttcggcaaa
     5821 ggagaatacc tggctgccac cgactatcag atggtccggc acccggatat ccaccagggc
     5881 cagcgcctgt accagacgtt cagtgataag ccggtctgcc ttactggggg tgacttcacc
     5941 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cacgtttaat
     6001 cacctcccgg ggatggactt ctgtgcggtt gatggtgcct gtgaagaggg tttcacccgc
     6061 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcccgct ccagtcctgc
     6121 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     6181 atgaagatgg cggtccaggg ttttcagggc ccgctgaatg aggctgcgct cgccgggcgt
     6241 catctctccg ggcagaaagg aaagctgttg cattgttccg gcccctttca gtcaatgatg
     6301 cgcataatgg cgttgcattc cggatgttgc agggcgtaat cccgcaaccg ataataatgg
     6361 accgtcatgg cataacactc cgtacgacag gcatgatgac tgtacgtcat cagacaggca
     6421 gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc attgaacagt
     6481 acccaggttt ctccatcatc ttcatccggt tcgggcgcca taaatgcgcc gccgttattc
     6541 agggtgtaca gattccagat accaccgcag tagtcttcac acagacggtc catccagccg
     6601 aagacacggg gctccagggt cacccactgt ggaatgaggc caaagtgctg cggccagaag
     6661 ctgatgcgct gttcatcagg gacgggagtg gcaaccagtt gagactggtt attccctgat
     6721 gcagtagtta cggaaacaga aggtgtggtg gaattatgcg aagaggttgt catgagatta
     6781 ttccttataa aaagcaaatg aatggaagaa tgcctgggga ctggtacaga cgtgagtcag
     6841 ggctgcactt tcagggaaac agcatcagcg catgctctcc agcagcgttt ctgccatcac
     6901 ccacaatgcg cggttgagct taatatcggt gtcgatgctg tgaatggcac gggtgtggat
     6961 acgttttcct ctggcactgc gaccggaaat tccgcctttc agcatattct cctggatggt
     7021 ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggtg tcagaatgtc
     7081 ggcagtggtg actggctgat gtttgtcacc ataacggtaa gtcagtgccg cctgtgccag
     7141 cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct tctcctctat
     7201 ccggtcaaaa acccccacca cctcgtaagc cccttcaata actttctcca ccacatttcc
     7261 ccggtgcgga acacgcactt cccccagaga ctgaccacag acgcatccgt tctggcagac
     7321 gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatgag agttgagcag
     7381 aataatttca gggacatgtt ctccgtttat ctctccggcc cgccgcagac gcagcatatg
     7441 tttggtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga agaacggctg
     7501 aaagccttcc cgctgcagac tttccagcac ggtgatggtg gggatatacg tatagcgttc
     7561 actgcgggag gtatgccggt cttccccaaa aataccgggt acatagtgca tcagttcttc
     7621 gtgtgtcagc ggacggtcac ggcgtatctg attcgcataa ccaaaacgac tggctaattg
     7681 catactttgc tccttatgaa tgaattagcg gtataaacga aaaggccgcg tctcccggag
     7741 aagacgcggc ctgatggatg aaatgaagac gtttattgcc tgagaattcc ctcactggcg
     7801 agatacgaat ttagctgttt tccggcatca ggttcaaaat tccagcaccg ccagcgtagc
     7861 tgcccttcgg cattgcggat aaccagccgg aaatgcgttc cctggtcatc ttcaataagc
     7921 acattgcggt aagctgttgt gacggcaacc gcctgttcgc gtgagaaagg gccttcgggc
     7981 aataaaacag ggtggcagtc ctacataccg gctattttcc gataaattca acgctggcgc
     8041 cggtggcatt accgtcagca ccaaccgtga cggtcacttt gtcgtacgca cggtcttcag
     8101 gattgaatac ttcaaactca accttattgc cttttacttc gtaagagcca aaatcagcgg
     8161 tttctgttgt ttcctcgtca tattcccggg cgtatgcctg ccgggcagcc tgcaggacgg
     8221 aatctttatt aatagtggtc gtctgtttcg gggaaaaacc ggcaagtgat gtgacaaatt
     8281 tcggacagac cttcagcata tcaccgcggg tgaattccat tcgtacacca aatgcctgta
     8341 aaacggacac atcacgctct gctatggagt gaacggcttt tttgaccatt tccggcgcct
     8401 gtccgatttc cacctcaaca gccctgaatc ccgtctgctg attacacatt tgtgatgtgg
     8461 ggatcaggtt accgttaata ctgatacggc tgcccaggag cgtctgtaca ccattcgggc
     8521 atccctgcat caggccggta ataccaatcg tgccattttt cagcattacc agcgcctgac
     8581 catcttccgt catggtctga caccagccgt ttttgacagc ccagttgccg gcggaggcgg
     8641 agaatgatgc cacagtgaac agcaggggaa aaagagctct ggtattcatg actgacctct
     8701 gaaatgaacg tacggtgtta ttgataatga tggaaggtat gcagtgactg ttcagggaat
     8761 gacgtagccg tatatttagt tttctgttca acatattgtc tccatacctg tcgggacggc
     8821 tttggcaggc cggcaatcac aggttaaagg tgttgataag tttatggatg aaagagaaat
     8881 gtggcagggc ggagataaaa cgctcttcag cgaccagact gtggatgtgg cctctccgtt
     8941 caaaccgttc tgatgtcagg ctgactgatt tgatatctgc cagaggaaag acctgcagtt
     9001 ttcccttctg taacgcgatc agataccagc taccgctgta ataaatgagc cggcagggct
     9061 ccagtgacgg ataccatcgc tcagtcatca gagagatgtg gatacactgt cttattgcca
     9121 ggatgagttg cgagaaacac tcggggaacg ttgcagtgat atccggcgcc ggtagccaga
     9181 taaggcaggg agagggctcc tggttgtcgg taagacaggt tatcagtcgt ccgttccgga
     9241 gcggaagtat ccgtgcgatc ccggtattct gtataaaaga aagcatttca gggatcgcac
     9301 ctgggctgct ctgtcgtctg aggctgtacc tgccatttct gtactctaaa tccaggtgaa
     9361 ccagacgctg atgaaaatcg cgctgtaaag tacgttctgt aacgccaaat tcatctgaca
     9421 gtgtttttag tgacagagat tctccggcca tcagtcggct gataatgagt gataagcgaa
     9481 ccgccaggcg atcatgccgg gaacgattat cagacataga tgtgcactcc gtaagaaaac
     9541 atcttttatt tcatgatgtt attatgcatc atgatgttga aaggatgtgt cgcatccgga
     9601 taatcaggaa taaaatgcag cagaaaaaat attcttttaa ttatctgatt tttaagcatg
     9661 atttcataaa aagaagaatg ctcgttaatc cagcgacaca tcctgtcggc gtgagtgtgt
     9721 cagaacagac gctcccacag tgtgatcagc aggtggataa tgcggtcccg ggctttacgc
     9781 agaagctgta acatccaggc cggaaaactg aaacgggcaa cggcgacgtc aaaacttttc
     9841 ccgatggttt caccaaaagt ctgttgtgcg tgtttctggg cctgttcaga gcggttttct
     9901 ccccggatat gtgaatacac cgcgctggtt gcctgtggtg gcagtgcgtg gatcatcagc
     9961 gacacaaacg ccggaaggtt ccagccgaca ggtgcagcaa cggagagtac cggaaatgat
    10021 gaagggaaca gggttgccac ccgggctgtc accgtcgcca gtgagagcgc ctgctgacgg
    10081 gacgggcatt tttctgtggc attccattct tcagcaggaa acacgcgatc ggcatggctg
    10141 agaacaaaca gaaaacgcga gggatctgcg ccttcattca gtaaaaactg atgcatggta
    10201 atgtcggcgg catacgcgcg ttcatctgcc cgcaggatcc agataatcaa atccacttcc
    10261 ggcagtaact gacggtaaag cgcccggtat tcctgatcat gttgcggcgt ctctccaatg
    10321 ccgggcagat cgaccagcgt cattctgcgt tcaccgatct gcagggtaag acgatgagcc
    10381 tggcgggtgc agccgttcag gggatgtgtg gcacagatat gggactgaaa aatagcatta
    10441 cacaggctac ttttgccggt gccggattta cccataatgc caatcacggg ttcgtaatga
    10501 ataacgctgt ggagatgttc aagaattttc tgacgaattt cttcaggaag aaacgtcaga
    10561 tatgatttca cttgtgcgga gagggaagaa tggctgaatg acataccggt atcctgttga
    10621 tttcactgaa ccggatcaca ctaatccggt ttatcattca ggtaatatgg acgtgaaaaa
    10681 tgatggaatg tctgaattac ggacattatt gttaaagtgt aatggagtat gaagtgagtt
    10741 tttcagaacg ggaactaatg acatactgcg gtttcaggag atgagaggat gaccggacgt
    10801 aaaaacaaat gcgtcagaaa aaattgtaac aatcaggttt tctcagtaaa aatggcagtg
    10861 gagaaaagac tgtgcagaaa acattatgag aggttttgtg agctcaaaaa aagaagagag
    10921 caaaatcctg ttcacagatt aaatgaagca ttgccaagaa agataaaagg acccaaaagt
    10981 aagaaaaaat tagtaaaaga aagagaaatc attaacagca ggaagaaaca taaacaggag
    11041 cagggaccat cgctggttga acttaataaa aaagttcgtg agatactgaa aactccggca
    11101 tggagaacgc tggaaacgag aattcgaaga aacggtatca catttaaatc aaaagcagag
    11161 ttagatgtac tttctgaact ttatgcaatt tattcatggg tttcatattg caaacggagc
    11221 gataattatt ctgtgtggtt tttgaattgt ccggatgtaa atataaatct tcatgtgtgc
    11281 gaatgcaggt cttctgaaag cgattgttat ctaccatccc gttttattat tcttccagct
    11341 ccattaaggc aaaaagtaaa aagaaaatac cctgctaatt cgtctggagt gtcaggatat
    11401 agtgattcaa aaagtcttta taagactgtt ttgtctgttt gtggtgggaa aagagaaacc
    11461 gaattgttct tttattcact gccagtaaag actaatcatc atccttactg gattaaaagt
    11521 ggtgtggtta aaagatttcc gcctattttt ggcttgttgt tttatgaaca ggaaaggtta
    11581 acaggaggtg tttttcgtta t
//
LOCUS       DAPPNJ010000143        11539 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000143 DAPPNJ010000000
VERSION     DAPPNJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.144"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(17..1360)
                     /gene="gdhA"
                     /locus_tag="RPM73_003242"
     CDS             complement(17..1360)
                     /gene="gdhA"
                     /locus_tag="RPM73_003242"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDX6521576.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1596..1868
                     /locus_tag="RPM73_003243"
     CDS             1596..1868
                     /locus_tag="RPM73_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDX6521577.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(1834..2241)
                     /gene="nudG"
                     /locus_tag="RPM73_003244"
     CDS             complement(1834..2241)
                     /gene="nudG"
                     /locus_tag="RPM73_003244"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDX6521578.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            2328..2948
                     /locus_tag="RPM73_003245"
     CDS             2328..2948
                     /locus_tag="RPM73_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDX6521579.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(2957..4264)
                     /gene="ynjE"
                     /locus_tag="RPM73_003246"
     CDS             complement(2957..4264)
                     /gene="ynjE"
                     /locus_tag="RPM73_003246"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDX6521580.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADALVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4331..4984)
                     /locus_tag="RPM73_003247"
     CDS             complement(4331..4984)
                     /locus_tag="RPM73_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDX6521581.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            complement(4984..6519)
                     /locus_tag="RPM73_003248"
     CDS             complement(4984..6519)
                     /locus_tag="RPM73_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521582.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLVWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     QWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDRSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(6492..7658)
                     /locus_tag="RPM73_003249"
     CDS             complement(6492..7658)
                     /locus_tag="RPM73_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX6521583.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEAKLLQTGWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDEQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7668..8216)
                     /locus_tag="RPM73_003250"
     CDS             complement(7668..8216)
                     /locus_tag="RPM73_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDX6521584.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8216..8923)
                     /locus_tag="RPM73_003251"
     CDS             complement(8216..8923)
                     /locus_tag="RPM73_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDX6521585.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIEHFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(8937..9614)
                     /gene="ydjY"
                     /locus_tag="RPM73_003252"
     CDS             complement(8937..9614)
                     /gene="ydjY"
                     /locus_tag="RPM73_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDX6521586.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(9619..10329)
                     /locus_tag="RPM73_003253"
     CDS             complement(9619..10329)
                     /locus_tag="RPM73_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDX6521587.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTITFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(10496..11302)
                     /gene="xthA"
                     /locus_tag="RPM73_003254"
     CDS             complement(10496..11302)
                     /gene="xthA"
                     /locus_tag="RPM73_003254"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDX6521588.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2788 a         3068 c         2988 g         2695 t
ORIGIN      
        1 atcaggcatt tacaacttaa atcacaccct gcgccagcat cgcatcggca accttcacaa
       61 aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc accttcacca ccatgctcaa
      121 cacaggcatg gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact ttctcggctt
      181 tccagcccag gcgcgcagcg ttttgcgcca tttcaaggcc tgacgttgct acaccgccag
      241 cattagccgc tttacccggt gcaaacagta cgcctgcctg ctggaacagt tcagtcgctt
      301 cgatggtggt cggcatattt gccccttcgg cgacggcttt aacgccattg gcgataagct
      361 gatgcgcggc gtcaacatcc agttcgttct gggtggcgca aggcagggcg atatcaaccg
      421 gtacagacca cggctgttgg ccttcgagat agaccagacc aaattctttg gcgtaatctg
      481 ccactcgacc atcgcggctg gctttgattt cgataagacg tgccagtttc tctttcgtga
      541 atccgctttc atcaactaca gtgccgctgg agtctgacgc agtgatcaca cgagcaccaa
      601 attccatcgc tttttcgata gcgtactggg cgacgttgcc ggagccagaa acggaaacgc
      661 gcatcccttc aaaacccata ccgtggcgtt ttaacattgc ttctgtgaaa taaaccagac
      721 cgtagccggt agcttccggg cgaataagac taccgccaaa tgaaaggccc ttaccggtga
      781 agacgcaggc ggtattgttg gagagctttt tcatcatccc cgccataaag ccgacttcac
      841 gaccaccaac gccgatatca cctgccggaa cgtcggtatc cgcgcccaga tgacgataaa
      901 gctcggtcat cagcgcctgg cagaaacgca tcacttcgcc ttcgctttta cctttcgggt
      961 caaaatcgct accgccttta ccaccgccca tcggcagggt agtcagggca tttttgaagg
     1021 tttgttcgaa gccgaggaat ttgagaatgg agaggttaac tgacggatgg aagcgcatac
     1081 cgcctttgta cgggccgatg gctgagctga actgcacacg ccatgcacgg ttgacctgta
     1141 cctgattgcg atcatcaacc cataccacgc gaaactggat cacgcgctcc ggttcaacca
     1201 gacgctccag taatgacatc tggcgatatt ttggattttg ttcaagaaaa ggccagagtg
     1261 tggtcattac ttcacgaacg gcttgcgcga actcggtttg attcgggtcg cgcttttgga
     1321 catggttgag gaatgactcc agagaatatg tctgatccat agatataaaa cccttatata
     1381 ttaatacgat tgtgcttatg ttgtttatgt gatgtgcttt tgcagttttc gactatacca
     1441 tcaagaatgt gacggtaagc aagaaaaaaa tgacccagga aagcaaatta ataacgagag
     1501 taatctcata accaaatgcg ataactcaga cgctaaagtt ttgccacccg gctaccgtta
     1561 tcagtagcag agacaataac gttaaggagt tccctgtgag tcgagcattg ttcgccgtag
     1621 tattagcatt tccattaatt gcactggcta acccgcatta tcgtccggac gtggaggtaa
     1681 acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc gcagccctgt actcagtgct
     1741 gcgtttatca ggataaaaat tattctgaag gcgcggtaat taaagccgag ggaatactgc
     1801 tgcaatgcca gcgggatgac aaaaccctta gcactaatcc gctggtctgg cggcgcgtaa
     1861 agccataaac gcctctaata atggaatgtc agcaggggcc agcggatatt gcagcgcctc
     1921 ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc
     1981 gggtacgtgc caggcatgaa gatggataat ccggcccgaa acttctcgct gatggctggt
     2041 aacatactca cccacagttg cttcgatgcc cagttcttcg cgtaactcac gcaccagcgc
     2101 ctgccgctgg ctttcatccg gctcgacttt accaccggca aactcccata atcccgcctg
     2161 atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc
     2221 aacaacttca atcattttca taaattacac acgggcagat aagcgaaaaa tgccataata
     2281 tccgcactgt tgctcaatca gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc
     2341 atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg
     2401 gcattacgcc agatggttta acattagtgg gatttgccat cggcgtgctg gcactgcctt
     2461 ttctggcgct gggctggtat ctggcggcgc ttgtcgtcat tttgttgaac aggctgcttg
     2521 atggtctgga cggtgcgctg gcgcggcgca gagggcttac cgacgcgggc ggctttcttg
     2581 atatttctct cgattttctt ttttacgcgc tggtaccttt tgggtttatc ctcgccgcac
     2641 cgcagcaaaa cgccctggca ggcggctggc tgctgtttgc gtttatcggc acaggcagca
     2701 gttttctcgc ttttgctgcg ctggcggcga aacatcagat tgataatccc ggttatgcgc
     2761 ataaatcgtt ttattatctg ggcgggttaa ccgaaggcac agaaacgatc ttactgtttg
     2821 tgctgggatg tttattccct gcatggtttg cgtggtttgc gtggatcttc ggtgcgttgt
     2881 gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgcaccagc
     2941 gtcagtaatt gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat
     3001 tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac
     3061 ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg
     3121 aaacttgctg ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc
     3181 tgcgcatggt gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt
     3241 gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg
     3301 taccgataaa ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca
     3361 gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga
     3421 aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt cccgcgtcgg
     3481 accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg tagagcataa
     3541 tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat
     3601 ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca
     3661 gcggttcgct ttccacttcg ttggtatcga tgtagtcagc accgggaata tggctgataa
     3721 ggtaaagctt aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg
     3781 ccgtaacctc tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa
     3841 agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat
     3901 gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt
     3961 acagcgccac cagggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt
     4021 cggtgctcat tttgtcgagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag
     4081 aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg
     4141 ctttgccatt ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc
     4201 acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt
     4261 tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa
     4321 aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgctccata tccagaacag
     4381 aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt
     4441 cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc
     4501 tgaatggctc atccaggagt aacgcttttg gctgagcgag aagggcgcgc agcagggcca
     4561 cccgcgctcg ctgaccgcct gataatgtcg cagggtcctg gtgataagtt tcagccagtc
     4621 ccgctcgatc aagcgcgtct ttcacggcat tgcgcctggc agtccccttg agcgtcgacg
     4681 gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt gcatcctgga
     4741 aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc
     4801 atagctcacc tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag
     4861 tggattttcc acagccagac ggccccatta acgtgacaat gtcaccttta tccaccgtaa
     4921 agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga
     4981 gcattagcgg agtccttgtc tgacataacc tacccatttt gcgactaacg cggtcagggc
     5041 aaaaataata agcggtaata gcagttgcca taaagcctgg gcggcgaaaa taccattgct
     5101 gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag
     5161 ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag
     5221 cgtagggcgt agcataagcg gacatttcac gtaaaagaag attttggccc gcgaccagct
     5281 cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat
     5341 aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg tccagcttct
     5401 atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcgggcaa
     5461 tagaatgggc aaccatagcc acaactggcg acgctgtggc ccccattcca gccactgcag
     5521 taacaggagc aaagcgatga atgttgctac cagccccatt gtcagactgt tgatgagtgc
     5581 ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc
     5641 ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg caggcttacg
     5701 aacgccatct acgcggggaa tagtacgccg ccagctgcgc catagcagat agctcagcaa
     5761 aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt tcgtttgttg
     5821 atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc
     5881 tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa tcgccagcat
     5941 cgccattgcc agcgcaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg
     6001 gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt cgctcaatac
     6061 tgccgctaag atccacacca gaaatgcgct ttctttcact gcaagggtga ggcccagacc
     6121 gatgccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata gcagccctcc
     6181 gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     6241 cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca caatcaccag
     6301 cagggcaatc aacaatgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa
     6361 tgcatgcggt aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaacgcagg
     6421 cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca
     6481 cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag tgcattcacc
     6541 catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat
     6601 atttcgcgct gctcgtcagg cagtttttgc ggatcgagaa cagaaggatc gccccagaca
     6661 gcgggatctg ctttacgcag ttgcgcatcg ggtgagagca ggaaattggc aaccaccttc
     6721 gccgcagcac tggcattcgc gttagcagga atggtgacga aatgcacgtt gccaatcatc
     6781 ccctcgttaa aaccaaaact gtaactgctt gcaggcaaat cgccgctggc gattttttgc
     6841 tgggcatgag cagggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     6901 tccatccgcg cgggcgaagg cgggaaatcc tttcctttgc gccataaata gggatgtagc
     6961 gcgtcaagat attgccacaa gggcgcagtg acgcgggcga aagtggcttt atccggggca
     7021 acttttaacg cggcgggatc gggcgtcagc ataatcagca actgttcaag aaacgccgtg
     7081 ccggtaaagt ccggtgggcg cggataggta accgtgccgg gattggcttt agcaaactcc
     7141 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaatgtc
     7201 agttgtgcgc ccccccaggg cgattcagcc ccttctgtag gcactgaaaa atcttcctgc
     7261 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     7321 aataacttgg cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc
     7381 gagccgcccg ttttacgtcc ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg
     7441 gcatccgcca gacggacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta
     7501 acccagtcga gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt
     7561 tgccctttgg cctcattttt aatttcctgc cagtcactgg catgtgttgc cagagaaaac
     7621 agcgataaca atcccagcaa ccacccacaa tggcgcatca tctctcctta agaacgtttt
     7681 tccggaatac ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgggctgac
     7741 aggttttgca ggccgatcaa tgccgtcagt tcggtgagcg cctgagcgtc aaaatgcgca
     7801 gccagtcggg tgcgcagggc atcatcgacc gttggcggcg taacgcttgc ggcttcggcg
     7861 tactccagcg ccagccgttc ttcatcgcta aagagcgggc tttgtcgcca gtcagctacc
     7921 gccagcaatt tatcggtgct gccggtgcgc tcggcgactt tcatactggt gatatccacg
     7981 caaaactcac acaggcacat ttgcgcgata cgcgcgctga caagcgatcg cactaccgga
     8041 tcgagcggtg agcgtttgcg ctccagccag ccaacaaaca tcgacaccag ataaaagata
     8101 aacgggatcc gcccccacca gcgaatgggg cttagcacct cgccgtaatg gcgacgatgc
     8161 agccacgcct gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagg
     8221 cattcctctt ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg
     8281 caaacagaat aaacagcccg gtgacaaacc agaaagtgcc gcccgttaac atgctacccg
     8341 cccaggagta gacgatggtc gcgggtaact gaccaagccc ggtggcgata aaaaacgagc
     8401 gaaaacgtat tgaagtcaaa ccggcagcat agctgattgg atcgaaaggg acaaaaggca
     8461 ataaccgaca gaccaggatg gtgtgtttgc cgtagcgagt gaaaaagccg tccatactgt
     8521 caagtacggt tttgccggtt aatttttcca ccacttcgcg gcccatcact ctggcgataa
     8581 aaaagcacag cgccgcgccg gccatcgaac tggtccacga cagcaagccg ccccagaacg
     8641 cgccaaatag cgacgcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg
     8701 cctgtaaaat catcaacaag aacgagacaa ctgccgccag tgcgccgtat gactgaataa
     8761 agtgttctat accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact
     8821 catggacgcc gggaagcagc gcccatgcca gcagcatagc gaacaacagg atgatgaggg
     8881 ttatacggta gtaccagatt ttacgcgatt gcatcttcat catcctttat cctgctttat
     8941 tcggtgattt taaaggtaac cgttgccagc gtgttatccg ccgggagaac tgaggcattg
     9001 cctttgaatt tcacttcgcc gcgtttttcc accgcaccat aagtgtatgt tgcattgctg
     9061 acgatgccga ccgggcagct atccagacac accaggcaac ccgttttctt ctcttctgct
     9121 gccgttaaat taccgccaaa gcgcatgtcc agtttcttgc cattactgtc aacaatcact
     9181 tcatcgaagg aatacgcttt tgccgcccct tgccagttga ccgaaatatc cagtttgcta
     9241 cctgtgacat gagtcgtttc tttattatcc atcgtcatgt tttcgcccgg cgtaccacct
     9301 gcctctttca gggcttcata aaatgctttc ggggttgcat aacccataaa cagcgattta
     9361 tgtccgttgg agccatcttt aaacacaata ccgtgacgag tgtcatcggt gaggaaacga
     9421 ccattaattt gcaccagcat ggttaccgta tggttattgg catcgacacg cagcggttgg
     9481 ctgttcgaaa gtccgtcgta ttccactttt gccgccgcat tctctttttg atcgcagccg
     9541 ctaagcccgg caacagccag taagcagagt gcagccagtc cttttttcca tgacactgaa
     9601 taatgttgcg acatcgttct atccttcatt ttttgggtga gtaagtgggc tgcggcgcga
     9661 agcagacaga tccacatgtt tgtgtcgggc gtagagtttt gcgagctgga cgagaataaa
     9721 cagcgccagg cccgccagac agagttgtaa aataaagcgc agcgtaatgc cttcattggc
     9781 gagatcgctt gccatcacgg tataaataac aataccgggc agggtcgtga gtgccgaaat
     9841 aagggtataa ggccagaagg tgattgtggt taatccgtaa gcgtaatttt gaatattgta
     9901 aggaaacaac gggattaagc gggtcagaat aagaaaatca ataccgttac gcgcaatgcc
     9961 tttttcaatg gcctgaaagg tatggctatg accaacgtat ttcagcagta aatcacgtcc
    10021 cagccagcgc gccagcagga atgagcacga ggaggcgagc gtggcggcaa ttaatgagag
    10081 tagtgtccct aagagcgggc caaaaactat tcctccagcg atcaccagta tgcttcctgg
    10141 taacagaaag agagtggcaa taatgaataa cagaatatag aggctataac cgaacaatcc
    10201 gctctggcgg atgagtgtct gtaagtgggg taaatcggtg agcagatcga ataaaccgaa
    10261 agtgtggata gcgtaaatga ccagcgcaaa aataagacag gccaaaagaa atttacgctc
    10321 agcgttcatc gtggagtttc ctatatcaaa cttgataata gagaagtggt cagacaacta
    10381 aaaacgcagt gacggtataa caaaggacgg caggcaacaa atcattactg acagtttgtt
    10441 gatttttctc aaggttaatt ctcctgaccc agtttgagcc aggagagctg ctaaattagc
    10501 ggcggaaggt cgcccagacg ggggcgtgat cggacggttt ttccatgctg cgaatttcat
    10561 agtcgatgcc ggtttctaca caacattctg ccagcggttg gctggcgagc agcaggtcga
    10621 tgcgcagacc acggttatcg tcaaaacctt ttgagcggta atcaaaccat gagaaacgat
    10681 ctgctgtttg tggattcgca tggcggaagg tatcgaccaa cccccagctc atcagcctgt
    10741 ccatccattc gcgctcttcc ggcaggaaag agcatttacc ggtacgcagc cagcgcttac
    10801 ggttttcttc gccaatgccg atatccagat ctgtagggct gatattcata tcgcccataa
    10861 tcagtaccgg attatcacgt ttgagttcgg tttccagata gttttgcaga ttctgataaa
    10921 actgcgcctt tgccgggaat tttatcggat ggtcgcggct ttcaccctgc gggaagtaac
    10981 cgttgatcac ggtgacattg cccagcggcg aggggatttc cgccataata attcgccgct
    11041 gcgcttcttc gtcgtcaccg ggaaaaccgc gacgcacggc aatcggcgtc tctttggtca
    11101 gcagcgccac gccataatgg cctttctgcc cgtgataaaa cacgttgtag ccgagcttcg
    11161 ccacctcttc gagcggaaac atatcgtcat gaacttttgt ctcctgcagg ccaatcacat
    11221 ccggttggtg cttttcgacg atggcttcaa gctggtgagg tctggcgcgc aggccgttga
    11281 tattaaaaga gacaaatttc atagtcgctg ccatttattc agataaagag tgcgtggatg
    11341 gtagcagaat ttcgcgttgc ttaccgcctg ttgttaagtg atttgatcga tggttgttaa
    11401 tgatgtcaac gatggcgcaa aaaatgcccg cttttggtgc gcgctgcgtc agaatggcgc
    11461 agtaatttcc agtaaatttc gtcaatgatc acaattccag aattatattt tacttctgca
    11521 taacattgcg ttttttatt
//
LOCUS       DAPPNJ010000144        11475 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000144 DAPPNJ010000000
VERSION     DAPPNJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.145"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..158)
                     /locus_tag="RPM73_003255"
     CDS             complement(<1..158)
                     /locus_tag="RPM73_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HDX6521589.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDR"
     gene            477..1058
                     /gene="mdaB"
                     /locus_tag="RPM73_003256"
     CDS             477..1058
                     /gene="mdaB"
                     /locus_tag="RPM73_003256"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDX6521590.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1089..1403
                     /locus_tag="RPM73_003257"
     CDS             1089..1403
                     /locus_tag="RPM73_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDX6521591.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1451..3343)
                     /gene="parE"
                     /locus_tag="RPM73_003258"
     CDS             complement(1451..3343)
                     /gene="parE"
                     /locus_tag="RPM73_003258"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDX6521592.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3372..3953)
                     /gene="yqiA"
                     /locus_tag="RPM73_003259"
     CDS             complement(3372..3953)
                     /gene="yqiA"
                     /locus_tag="RPM73_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDX6521593.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3953..4780)
                     /gene="cpdA"
                     /locus_tag="RPM73_003260"
     CDS             complement(3953..4780)
                     /gene="cpdA"
                     /locus_tag="RPM73_003260"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDX6521594.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4805..5227)
                     /locus_tag="RPM73_003261"
     CDS             complement(4805..5227)
                     /locus_tag="RPM73_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDX6521595.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5228..5857)
                     /gene="nudF"
                     /locus_tag="RPM73_003262"
     CDS             complement(5228..5857)
                     /gene="nudF"
                     /locus_tag="RPM73_003262"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDX6521596.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6062..7543
                     /gene="tolC"
                     /locus_tag="RPM73_003263"
     CDS             6062..7543
                     /gene="tolC"
                     /locus_tag="RPM73_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDX6521597.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7691..8362
                     /locus_tag="RPM73_003264"
     CDS             7691..8362
                     /locus_tag="RPM73_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDX6521598.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLIAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8368..9528
                     /locus_tag="RPM73_003265"
     CDS             8368..9528
                     /locus_tag="RPM73_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDX6521599.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(9566..10354)
                     /gene="ygiD"
                     /locus_tag="RPM73_003266"
     CDS             complement(9566..10354)
                     /gene="ygiD"
                     /locus_tag="RPM73_003266"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDX6521600.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPMLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10497..11270
                     /gene="zupT"
                     /locus_tag="RPM73_003267"
     CDS             10497..11270
                     /gene="zupT"
                     /locus_tag="RPM73_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDX6521601.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT         2861 a         3082 c         2830 g         2702 t
ORIGIN      
        1 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
       61 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
      121 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcataaaaat
      181 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
      241 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
      301 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
      361 cagagctaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
      421 ccgtaccgct ctgtgacgta ctatagtcga caaacgtctc accttgaggt taaaaaatga
      481 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
      541 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
      601 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
      661 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
      721 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
      781 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
      841 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
      901 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
      961 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
     1021 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
     1081 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
     1141 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
     1201 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
     1261 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
     1321 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
     1381 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
     1441 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
     1501 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
     1561 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
     1621 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
     1681 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
     1741 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
     1801 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
     1861 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
     1921 cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
     1981 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
     2041 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
     2101 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
     2161 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
     2221 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
     2281 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
     2341 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
     2401 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
     2461 agaactcacg catcgcgtcc aacaggccct gacgcagacc gttaacgtgc gtaccgccct
     2521 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
     2581 acagtagcgc ccagtccaca gcttcagtat caccagcgaa attaccgata aacggttttt
     2641 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctggt
     2701 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccagggc
     2761 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
     2821 caaagaaggt ttcatccggc cagaagtgca cgctggtacc agtattgcgt ttaccgcaag
     2881 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
     2941 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
     3001 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
     3061 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
     3121 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
     3181 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
     3241 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
     3301 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
     3361 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
     3421 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
     3481 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
     3541 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
     3601 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
     3661 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
     3721 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
     3781 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
     3841 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
     3901 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
     3961 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
     4021 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatagta tccagcgtaa
     4081 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
     4141 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
     4201 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc
     4261 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
     4321 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
     4381 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacgcgct
     4441 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
     4501 tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat
     4561 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
     4621 gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg tttaccccta
     4681 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
     4741 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
     4801 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
     4861 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
     4921 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
     4981 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
     5041 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
     5101 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
     5161 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
     5221 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
     5281 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc
     5341 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
     5401 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
     5461 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
     5521 cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg gcaaccatct
     5581 ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca
     5641 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
     5701 tttcccgccg tacctcatga ctcatttgcc cgttgaatag acgatgacga aatctataaa
     5761 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
     5821 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
     5881 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
     5941 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta
     6001 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
     6061 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca gttcgttgag
     6121 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
     6181 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
     6241 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
     6301 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
     6361 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg acgtcacata
     6421 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
     6481 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
     6541 tcaaaccacc caacgtttta acgtgggcct ggtagcgata accgacgtgc agaacgcccg
     6601 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc ttgataacgc
     6661 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
     6721 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
     6781 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
     6841 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
     6901 cacctcttat agcggttcga aaacccgtgg tgccgctggt acccagtatg acgacagcaa
     6961 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
     7021 taactcgcag gtgaaacagg cacagtacaa ctttgtcggt gccagcgagc aactggaaag
     7081 cgcccatcgt agcgtcgtgc agaccgtgcg ttcctccttc aacaacatta atgcatctat
     7141 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
     7201 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg cgaccaccac
     7261 gctgtacaac gccaagcaag agctggcgaa tgcgcgttat aactacctga ttaatcagct
     7321 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
     7381 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
     7441 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
     7501 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
     7561 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
     7621 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
     7681 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
     7741 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
     7801 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
     7861 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
     7921 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
     7981 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
     8041 cagcgggagt ttctggatgc cgctgatagc cggttacatg atggggcgtc tgatgggcgg
     8101 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
     8161 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
     8221 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
     8281 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
     8341 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
     8401 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
     8461 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
     8521 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
     8581 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
     8641 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
     8701 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
     8761 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
     8821 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
     8881 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
     8941 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
     9001 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
     9061 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
     9121 ggcgtacgct ggctggaacc agcgtggaag agcattatct ccaacaaggc acttctaccg
     9181 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
     9241 gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
     9301 gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
     9361 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
     9421 ggtagctggc tggtgaacga tcaacctgcc ggaattggca ttcgtgaaga ccgtgcgttg
     9481 atcacccagg atatgtctcg gttttatcca catattttta ttgagtaatc aatgatgccg
     9541 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
     9601 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
     9661 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
     9721 cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga ttcgctttca
     9781 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
     9841 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
     9901 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
     9961 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
    10021 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggcgctaac agctcaacca
    10081 gatgctgtgc cagcataggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
    10141 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    10201 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    10261 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    10321 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    10381 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    10441 gatcatcagt tattttgacg atctgcctga aagtgaagat ttataaggag ttgtcgatgt
    10501 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    10561 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    10621 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    10681 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    10741 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    10801 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    10861 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    10921 cactggccgt cgctttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg
    10981 cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa
    11041 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
    11101 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    11161 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    11221 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
    11281 agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
    11341 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    11401 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
    11461 agagcaatca ataca
//
LOCUS       DAPPNJ010000145        11444 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000145 DAPPNJ010000000
VERSION     DAPPNJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.146"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..590
                     /locus_tag="RPM73_003268"
     CDS             <1..590
                     /locus_tag="RPM73_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDX6521602.1"
                     /translation="PAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGHNGEDF
                     LVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHT
                     GFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSIPW
                     ERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(592..1113)
                     /gene="ruvC"
                     /locus_tag="RPM73_003269"
     CDS             complement(592..1113)
                     /gene="ruvC"
                     /locus_tag="RPM73_003269"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDX6521603.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1148..1888)
                     /locus_tag="RPM73_003270"
     CDS             complement(1148..1888)
                     /locus_tag="RPM73_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX6521604.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1917..2369)
                     /gene="nudB"
                     /locus_tag="RPM73_003271"
     CDS             complement(1917..2369)
                     /gene="nudB"
                     /locus_tag="RPM73_003271"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDX6521605.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAVREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2362..4134)
                     /gene="aspS"
                     /locus_tag="RPM73_003272"
     CDS             complement(2362..4134)
                     /gene="aspS"
                     /locus_tag="RPM73_003272"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDX6521606.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4444..5010
                     /locus_tag="RPM73_003273"
     CDS             4444..5010
                     /locus_tag="RPM73_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX6521607.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5007..5825
                     /locus_tag="RPM73_003274"
     CDS             5007..5825
                     /locus_tag="RPM73_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDX6521608.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5878..6273
                     /locus_tag="RPM73_003275"
     CDS             5878..6273
                     /locus_tag="RPM73_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDX6521609.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6314..7057
                     /gene="cmoA"
                     /locus_tag="RPM73_003276"
     CDS             6314..7057
                     /gene="cmoA"
                     /locus_tag="RPM73_003276"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDX6521610.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7054..8025
                     /gene="cmoB"
                     /locus_tag="RPM73_003277"
     CDS             7054..8025
                     /gene="cmoB"
                     /locus_tag="RPM73_003277"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDX6521611.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8190..10619)
                     /gene="torZ"
                     /locus_tag="RPM73_003278"
     CDS             complement(8190..10619)
                     /gene="torZ"
                     /locus_tag="RPM73_003278"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDX6521612.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGKDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVASVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10644..>11444)
                     /locus_tag="RPM73_003279"
     CDS             complement(10644..>11444)
                     /locus_tag="RPM73_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDX6521613.1"
                     /translation="DSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQS
                     ESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPF
                     KTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSE
                     EGIKALTVNGEWQADEYGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHA
                     PIAADHYTVNAWPSIAKGMGVRTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2573 a         3084 c         2820 g         2967 t
ORIGIN      
        1 accctgctga atatgaaatt ggtgatatcg tctttacatg tataggtgct gccttatttg
       61 gtcaaatatc agctgcatca aactgctgga gtaatcacgt cgggatcatt atcggtcata
      121 acggtgaaga ctttctggtt gcagaaagcc gtgttcccct ctcaaccatc actacgctat
      181 cccgttttat taaacgctct gctaatcaac gctatgctat aaagcgatta gacgccgggc
      241 tgacagaaca acagaaacaa cgaattgttg aacaggttcc ctcccggcta cgcaaacttt
      301 accacacggg ttttaaatac gaatcttccc gccagttctg ttcaaaattt gtctttgata
      361 tttataaaga ggcgctatgt attccggtgg gtgaaataga gacgtttgga caactgttaa
      421 atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcttaggt tctattccgt
      481 gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg gtgttgattc
      541 acgcggaggg agttgaaacg cctcagcctg aactgaccga ggcggtataa cttaacgcag
      601 tcgccctctc gccaggttca gccgcgattc gctcatctgc attgcattct gactgacgtg
      661 acaatgggta atcgcgatag ctaacgcatc agcggcatcc gcctgtggat tagcgggcag
      721 tttcagcaag gtgcggacca tatgctgcac ctggcttttc tcggcgctac caatacctac
      781 caccgtttgc tttacctgac gtgccgcgta ttcaaatacc ggcaattcct gattcaccgc
      841 cgccacaatc gccacgccgc gcgcctggcc cagtttcagg gctgagtcag cgttcttcgc
      901 cataaagact tgttcaatgg cgaaataatc aggctggaac tgggtgatga tttccgtcac
      961 gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc gtatgcatcc
     1021 actacccagg taggacagtt gcctgcctac ctggcggatg acgccatagc cggtcacgcg
     1081 cgaacccgga tcaatgccga gaataatagc catcacgcgt ctccgttttg ctgtttagca
     1141 ggcctcatca gagagtcgct gcaacctcat cagagatttc accgttatgg taaacttcct
     1201 gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt gcggtttctg
     1261 catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct gctttcagac
     1321 ctgccgcttc cagagcgtcg cgcactttac ccatttcttc ccatgcagtg tagacatcaa
     1381 tcgcgccgtc atcataggtc acaacgtctt cagcacctgc ttccaaagct gcttccatga
     1441 tggtgtcttc atcgcctttc tcaaaggaga tcacgccttt tttgctgaac agataggcta
     1501 cggaaccatc agtaccgagg ttaccgccac atttgctgaa tgcatgacgc acttcagcaa
     1561 cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg ccaggaccgt
     1621 aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca ccacgtgcaa
     1681 ttgcgcggtt cagtgtgtca cgggtcatgt tgttagacag tgctttatca attgctgcac
     1741 gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt agccgcggtt accagctcac
     1801 gaatgatttt agtgaagatt ttaccgcgct ttgcatcctg cgcagcttta cgatgtctgg
     1861 tgttggccca tttactatga cctgccataa aaatatctcc agatagccct gcctgttcag
     1921 gcagcgttaa tgacaaactg ttcaatcgcc tgccggttgc tccaggactt agtgagcgcc
     1981 gccgcagcag gcgcatcaag ccacttgtaa gccagatgtt cagtgaaaac gatctgccgc
     2041 tcgtgcggaa gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc cggcgcatag
     2101 cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc aattaaggtc
     2161 agttgttcag cgacaacatc aatggtgacc tcttccttta cttcgcgcac ggcagcttgc
     2221 ggcgcggttt ccccctcttc cacgctgccg gttaccgact gccagaaatc gggatcgtca
     2281 cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt agatgaccac taagatcgaa
     2341 acgggacgct tataagccat atcagttatt ctcagccttc ttcacaacct gaatgctcag
     2401 ctcagccagt gcagtcgggt tagcaaagct cggtgcttca gtcatcagac acgctgccgc
     2461 cgtggttttc gggaaggcaa taacgtcacg gatattgtcg gtgccggtca gcagcatggt
     2521 cagacggtca agaccgaatg ccagacctgc gtgcggcgga gtaccgtatt tcagagcgtc
     2581 gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg atacccagaa taccaaacac
     2641 cgtctgctgc atatcaccat tatggatacg tactgaacca ccgcccactt cgtaaccatt
     2701 gatgaccata tcgtaagcgt tcgccaccgc attttccggt gcagctttca gttctgcagc
     2761 agtcatgtct ttcggtgagg tgaacggatg gtgcattgcc gtcaggccgc cttcaccgtc
     2821 gtcttcaaac atcgggaagt cgataaccca cagcggtgcc catttgcttt cgtcggtcag
     2881 accaaggtct ttacccactt tcaggcgcag tgcgcccatc gcgtcggcaa caattttctt
     2941 gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg gcagtacgat ccaggatggc
     3001 ttcgatgatt tctgcattaa ggaacttcgc taccgggctg ttgatacctt ccagaccttt
     3061 cgcgcgttcg ttaactttga tgtaagccag acctttcgcg ccgtagattt taacgaagtt
     3121 accgtattcg tcgatctgct tacgggtcag cgatgcgccg cccggaacgc gcagagcggc
     3181 aacgcgacct ttcggatcgt tcgccggacc tgcaaatact gcaaactcaa cagatttcag
     3241 cagatcagca acgtcggtca gttccatcgg gttacgcaga tccggtttat cagaaccata
     3301 acggcgttct gcttccgcaa aggtcattac cgggaaatcg cccagatcca cgcccttcac
     3361 ttccagccac aaatgacgca ccagcgcttc catcacttca cgcacttgcg gcgcggtcat
     3421 gaaagaagtt tccacatcga tctgagtaaa ttcaggctga cgatcagcgc gcaggtcttc
     3481 gtcacggaag catttaacga tctgatagta acggtcaaaa ccggacatca tcagcagctg
     3541 tttgaacaac tgtggggatt gcggcagtgc gtagaattta cctttgtgca cacgagaagg
     3601 caccaggtag tcacgcgcgc cttccggcgt ggctttggtc agcatcggag tttcgatgtc
     3661 gaggaagccg tggtcatcca taaaacggcg caccaggctg gtgattttag cgcgggtttt
     3721 caggcgctgc gccatttccg ggcgacgcag gtcgaggtag cggtatttca gacgcgcttc
     3781 ttcggtgttg acgtggttag agtcaagcgg cagaacatct gcgcggttga tgatagtcag
     3841 cgaggacgcc agcacttcaa tttcgccggt cgccatatcg cggttaatat ttttttcgtc
     3901 acgcgcacgt acggtgcccg taacctgaat gcagaactca ttacgcagtt cagaggccag
     3961 ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc acgatacctt cgcggtcgcg
     4021 catatcgatg aagatcaggc taccaagatc acgacgacgg ttgacccaac cacacagagt
     4081 cacctgctgc cccacgtggg acaaacggag ctgtccacaa tattctgtac gcatgagata
     4141 tcccttaact tagctgccgg cggatgcccc ctgctgcgca ggtgaccaag tcgcagcgtt
     4201 agctgtatgt cacaactgaa tgaaaaaagg cggctattat actggaaatt ctgccgcacc
     4261 gtaagagcct ggcccgcgct ggaacgcctc gttaccactt tatatcgggc ctgaaatcag
     4321 actctacgcc agtttgctat aaaggtgttg cccgaactca taaaaattaa caaaatttgt
     4381 cgttccgcca tcggctaatc gcattaaggt gtgaggcacg attttgtttt ttcaggagtc
     4441 agcatgcttg aacttaatgc taaaaccact gcgttggtgg tgatcgattt acaagaaggc
     4501 atcttacctt ttgccggtgg tccacatact gccgatgagg tagttaatcg cgccgggaag
     4561 ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc tggtgcgcgt tggctggtct
     4621 gccgatcacg ccgaagcatt aaaacagccg gttgatgccc cctcccccgc taaagtgttg
     4681 cccgaaaact ggtggcaaca tccagctgca ttaggtgcaa ctgacagtga tatcgaaatc
     4741 atcaaacgtc agtggggtgc gttttacggt acggatctgg agttgcaact gcgccgccga
     4801 ggtatcgata caatagtgtt atgtgggatc tcgaccaata tcggtgttga atccaccgcc
     4861 cgcaatgcct gggaacttgg ctttaatctg gtgattgccg aagacgcctg tagtgccgcc
     4921 agcgccgagc agcacaataa cagcatcaac catatctacc cacgcatcgc ccgtgtgcgt
     4981 agcgtggaag agatccttca cgcgttatga tttacatcgg tctaccgcaa tggtcgcatc
     5041 ctaaatgggt gcggttggga atcaccagcc ttgaagagta tgcccgccac tttaactgcg
     5101 tggagggcaa caccacgctt tacgccctgc cgaaacccga ggttgtcctg cgctggcgtg
     5161 aacagaccac agatgacttc cgcttctgtt ttaagtttcc ggcgaccatt tcgcatcagg
     5221 cagcattacg acattgcgat gatttagtga ctgaattttt gacccgcatg tcaccgttgg
     5281 ctccgcgcat tgggcaatac tggctgcaac tgcctgccac attcggccca cgggagctgc
     5341 ctgcgctttg gcattttctc gattctctcc ctggcgaatt taattatggt gtggaagtcc
     5401 gccatccaca gtttttcgcc aaaggggaag aggaacaaac gcttaatcgc ggtttacatc
     5461 agcgcggcgt taatcgggtg attttagaca gccgcccggt tcatgcagca cgtccacaca
     5521 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
     5581 cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
     5641 tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg ccttatcttt
     5701 ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg tgggaagact
     5761 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
     5821 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
     5881 gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
     5941 gttcgcttgc gtttgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
     6001 agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
     6061 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
     6121 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgttccg ctggcgacgt
     6181 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
     6241 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
     6301 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
     6361 cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc agcgttccgt
     6421 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     6481 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     6541 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     6601 acgctgccgt cgtcatattg acgcctataa agcccctacg ccagtagacg tcattgaagg
     6661 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca
     6721 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     6781 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     6841 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     6901 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     6961 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     7021 ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa cttttattct
     7081 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     7141 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt ggaatttctg
     7201 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     7261 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcgcaacct gatgccatgg
     7321 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     7381 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     7441 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     7501 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     7561 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     7621 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     7681 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     7741 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     7801 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     7861 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     7921 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     7981 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     8041 gccggatgcg gcgtgaatgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     8101 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     8161 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     8221 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     8281 caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     8341 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     8401 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     8461 accagatcgc cattcgcaat accaaatcga gcagcatctt cggtgtgaat agtgatcggt
     8521 tcacgatcgg caaccgcata ttttttacgc agtcccgcat agttaagctg actgtgtaaa
     8581 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     8641 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     8701 gttttggagt aaatctcaat tttgccactt ggcgtaccca gcgcattttt caccggatcg
     8761 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     8821 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     8881 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     8941 tagatctctt ttccgccagg tttcagtaat tccgcaagat cggcaaacac atcaaaatcg
     9001 tttcgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     9061 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     9121 tctgcatgtt tagccgctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     9181 tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
     9241 caccagatca ttttgatatt cggatacgtt tgctctttac cgttatgctg atatttgccg
     9301 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccctcat tccgccatca
     9361 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
     9421 acacgtgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
     9481 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
     9541 tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc cgccatcagt
     9601 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc ccaggccgcg
     9661 ctcttcggcg tattatcgct cttacctgtc agatactctt caaactgcgg atacccggtg
     9721 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
     9781 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
     9841 aagaattcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
     9901 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
     9961 gtattcagcg ggttcatccc ccacagcacc acaacctggc tgttttccag aatcagcggc
    10021 caggaggtct gctgttcata cacctctaca gaagcgacca catgcggcat gatcacctgt
    10081 gccgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
    10141 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtaagatccg
    10201 gcaaaaatgg ccgatggtcc gtttgcttta cgaatacgct catgttgctc atgaatcagt
    10261 tttaatgcct gttcccagct cacctgtaca taggtatctt tgccacgacc tttcgccggt
    10321 tgcagtggat tgtcgagata gctttttctc accatcggat gctgaatacg cgccgtggtg
    10381 tgtacctgat ccgccgccgt agactgtaag gagttcggta tggttttcgc cagcgcgcct
    10441 gtcgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca acgtcccgcg
    10501 gtgaggattt taccgccctt ctcttctgcc catgcgggta gcggtgctgc cgatgtcacc
    10561 accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt taatgtcata
    10621 acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc gttgtactgg
    10681 aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc tggttcgtac ccccattcct
    10741 ttggcaatgg acggccacgc attgacggtg taatggtcgg cggcaatagg ggcatgacaa
    10801 ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt acggtccgct
    10861 aatccgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt gccgtattcg
    10921 tcagcctgcc attccccgtt taccgtgagc gccttgatac cttcttcact taatgtggct
    10981 agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg catttgataa
    11041 ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag atcggtacca
    11101 ggattcacgg tagccagctc gcctatgcga gaagttttga aaggataaat atgtgcgcca
    11161 ttagtcactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc atccattttt
    11221 atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc gccgcccttt
    11281 tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc catggcatca
    11341 aaagaatggc aactacggca cgttgcagag tcagttgctt ttaattcttt ccatactgtt
    11401 tcggccattt cctggcgatg agtttcgaac ttatcgtcac tgtc
//
LOCUS       DAPPNJ010000146        11291 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000146 DAPPNJ010000000
VERSION     DAPPNJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.147"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            175..1746
                     /gene="garD"
                     /locus_tag="RPM73_003280"
     CDS             175..1746
                     /gene="garD"
                     /locus_tag="RPM73_003280"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDX6521614.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1895..2230
                     /gene="prlF"
                     /locus_tag="RPM73_003281"
     CDS             1895..2230
                     /gene="prlF"
                     /locus_tag="RPM73_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDX6521615.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2230..2694
                     /gene="yhaV"
                     /locus_tag="RPM73_003282"
     CDS             2230..2694
                     /gene="yhaV"
                     /locus_tag="RPM73_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDX6521616.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2749..3558)
                     /gene="agaR"
                     /locus_tag="RPM73_003283"
     CDS             complement(2749..3558)
                     /gene="agaR"
                     /locus_tag="RPM73_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDX6521617.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3807..5087
                     /gene="kbaZ"
                     /locus_tag="RPM73_003284"
     CDS             3807..5087
                     /gene="kbaZ"
                     /locus_tag="RPM73_003284"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDX6521618.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5110..5583
                     /gene="agaV"
                     /locus_tag="RPM73_003285"
     CDS             5110..5583
                     /gene="agaV"
                     /locus_tag="RPM73_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDX6521619.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5594..6373
                     /locus_tag="RPM73_003286"
     CDS             5594..6373
                     /locus_tag="RPM73_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDX6521620.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6363..7241
                     /locus_tag="RPM73_003287"
     CDS             6363..7241
                     /locus_tag="RPM73_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDX6521621.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVIHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7259..7693
                     /locus_tag="RPM73_003288"
     CDS             7259..7693
                     /locus_tag="RPM73_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX6521622.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7690..8823
                     /gene="nagA"
                     /locus_tag="RPM73_003289"
     CDS             7690..8823
                     /gene="nagA"
                     /locus_tag="RPM73_003289"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDX6521623.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9174..10328
                     /locus_tag="RPM73_003290"
     CDS             9174..10328
                     /locus_tag="RPM73_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDX6521624.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10341..11201
                     /gene="kbaY"
                     /locus_tag="RPM73_003291"
     CDS             10341..11201
                     /gene="kbaY"
                     /locus_tag="RPM73_003291"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDX6521625.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2706 a         2925 c         2984 g         2676 t
ORIGIN      
        1 gcacagattt gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg attgacattg
       61 atcacatttc tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt
      121 ttaaatgaga ccaggtcctc attttaataa cccctggctg gagaatattg cacaatggcc
      181 aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat
      241 aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatgga
      301 ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga cattccggct
      361 aatggtgaaa ttattcgtta tggcgaagtg attggttacg ccgtgcgtgc aatcccacgc
      421 ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg
      481 ccactggcaa ccaaagtccc ggccccctta ccgccgctgg aaggatacac ctttgagggc
      541 tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc
      601 cactgtgtgg caggcgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg
      661 aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg tggcgtggcg
      721 attaacgcac cggcggcagt tgtgcctatt cgtaccatcc acaatatttc gctgaaccct
      781 aactttggcg gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca gcctgagcgc
      841 ctgctggtcg gcacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt
      901 ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt gcaggtcgcc
      961 gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg
     1021 gttgtcggca tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc aaacccggcg
     1081 gttggctatg cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ctcagaagtc
     1141 acggaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc
     1201 aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac
     1261 cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt ggtagagaag
     1321 gcactcggct ccattgctaa atcgggtaaa agcgcaattg ttgaagtgct gtcgcccggt
     1381 caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt
     1441 ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg
     1501 ccgtacggcc tgatggcggt acccgtcatt aaaatggcga cccgcaccga gctggcgaac
     1561 cgctggtttg atttaatgga tattaacgcg ggcactatcg ccaccggcga agaaaccatt
     1621 gaagaggtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa
     1681 accttctcgg atcaatgggg gctgcataac cagctggcag tgtttaaccc ggcaccggtg
     1741 acctgatttc ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt
     1801 ttaaagcccc cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt
     1861 gagcagaaaa caggctgtaa aaggacagtg aatcatgccc gctaatgctc gctctcacgc
     1921 tgtactgacc actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt
     1981 gcgtgaggcc ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcctgg
     2041 tgggcaagtc tttatgtgcc gactgggaga tgaacaggag gatcatacta tgaatgcatt
     2101 tttgcgtttt ctggatgcag atatccagaa caacccgcaa aaaactcgtc cattcaacat
     2161 tcaacaagga aagaaacttg tcgctggcat ggacgtcaac attgatgatg agattggcga
     2221 cgacgaataa tggattttcc acaaagggtt aatggttggg cgctatatgc tcatccctgt
     2281 tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga cattaaaggg aaaagatcct
     2341 gaaaattatc agagaaaagc cgccacaaag ttattggcgg tagtccataa agtgattgag
     2401 gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc gttaggctct
     2461 gggaaaaata aagactggtc acgggtaaaa tttggtgctg gtcgttatcg tctcttcttt
     2521 cgttatagtg aaaaagagaa agtcatcatt ctgggatgga tgaacgatga aaacactctg
     2581 cgcacctacg gtaaaaaaac agatgcctat accgtattca gcaaaatgtt aaaaagagga
     2641 catcctcctg ccgactggga aagcctcacc caagagacag aagaaaacca ttgatggtgt
     2701 tcacaatgcc cgccgaacgt attcgtgttg accagcgggc accctttctc actccccgac
     2761 cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag gaatgccttc
     2821 atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac tggaacgatt
     2881 gaatttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca tccgacggtt
     2941 taaacgggct tcatcttcat tatgtgtgct gacgccgcgc tccagatcga tcgcatctac
     3001 accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct gatcgccgta
     3061 aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa cgccttccgc
     3121 ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt cagtgtgctt
     3181 acgcatcaga cgagcaatct caaaagtggt agtcccggaa tcgaggatca cccgatgacc
     3241 aggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg tgttcagcgc
     3301 gcttttatct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga tcaacgcgcc
     3361 accataggca cgcacagcga tcccctgctt ttccagaaac gccagatcat tgcggatcgt
     3421 cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc cttgctgtcg
     3481 cagacgctga atgatctgtt ctcgtcgctc gctggtgcca gtcactcgct tctcacctga
     3541 agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc
     3601 tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattgacg caagctactc
     3661 tcgtttcagt gactttcatt atgtttcttt tgtgaatcag atcagaaaac tattatcttt
     3721 cgttttattt ttatctcacc atgacgcagt atcaactgaa acaaaacgaa agattaatat
     3781 cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg agacagcaca
     3841 aagcgggcaa aacaaatgga atttatgccg tttgttccgc acatccgctg gtgctggagg
     3901 ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact gattgaagca acctccaatc
     3961 aggtagacca gttcggcggt tataccggaa tgacgcccgc cgattttcgc ggctttgttt
     4021 gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt gattctgggt ggtgaccatc
     4081 tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc aatggccaat gccgatgatt
     4141 tgattaaaag ctacgttgcg gcaggattca aaaaaatcca ccttgattgc agcatgtcct
     4201 gtcaggacga tccgattccc ttaactgatg acatcgtggc tgaacgcgcc gcccgtctgg
     4261 cgaaagtggc ggaagaaacc tgtcttgaac actttggcga agccgatctg gagtatgtca
     4321 ttggtaccga agtgccggta cctggcggcg cgcatgaaac cttaagcgag ctggcggtca
     4381 ccacgccgga tgccgcccgc gccacgctgg aagcccatcg tcacgccttt gaaaagcaag
     4441 gtttgaatgc catctggcca cgcatcattg ccctggtggt tcaacccggc gtcgaattcg
     4501 atcacaccaa cgttattgat tatcagcccg ccaaagcgag cgccttaagc cagatggtcg
     4561 aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg ccgcaatcgc
     4621 tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccggcg ctgaccttcg
     4681 ccctgcgtga agctctgttc tctctggcgg cgattgaaga agaactggtg ccagcgaaag
     4741 cctgttctgg tctgcgtcag gtgctggaag acgtgatgct cgaccgcccg gaatactggc
     4801 aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg
     4861 atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct catctggttc
     4921 gtaatctggc ggattcacca attccgctac cgctcatcag ccagtatctg ccgctgcaat
     4981 acgtgaaagt tcgctccggc gagctgcaac cgacgccacg ggaactcatt atcaaccata
     5041 ttcaggacat cctggcgcag tactacacag cctgtgaagg ccaataagca aaacaaagag
     5101 gaacacgcta tgccaaatat tgttttaagc cggattgatg aacgcttgat tcacggtcag
     5161 gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg tgctggtagc caacgatgag
     5221 gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga agggatcgcc
     5281 gtacgtttct ggacgctgca aaaagttatc gacaacattc atcgcgccgc cgatcgacag
     5341 aaaatcctgc tggtttgtaa aacacccgcc gatttcctga cgctggtgaa aggtggcgtt
     5401 ccggtgaatc gcattaacgt tggcaatatg cactacgcca atggcaaaca acaaatcgcc
     5461 aaaacggttt ctgtggatgc gggcgatatc gcagcattta acgacctgaa agccgctggg
     5521 gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt taaattactt
     5581 tgagggattc atcatggaaa tcagcctgtt gcaggcattt gcgttgggca ttatcgcctt
     5641 tatcgctggc ctggatatgt ttaacggcct aacacatatg caccgcccgg tggtcctcgg
     5701 cccgttggtc gggctggtac ttggcgatct gcataccgga attttaaccg gcggtacgct
     5761 ggaactggtg tggatggggc tggccccgct ggcgggcgca cagccgccta acgtgattat
     5821 cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg atgtcgcggt
     5881 cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg attaccttcc tgttctcggt
     5941 gatgtctggc gtgatgtctc gctgcgaccg gatggcggag aacgccgata ctcgcggcat
     6001 tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc tctgcgcttt
     6061 cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg
     6121 attaatcgac ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct
     6181 gctgaaaatc atgatgaaaa acgtctacat cccctacttc atcctgggct ttgttgccgc
     6241 agcctggctc aagttaccgg tgctggctat tgctgccgct gcgctggcga tggcgctgat
     6301 cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag aggaatttga
     6361 agatggcatc taatcaaacc accctgccga acgtcagtga aaacgaagaa acattgctga
     6421 ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg cgcggagctg acgaaaaaag
     6481 atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac tacgaacgta
     6541 tgcaggcttc tggctggttg tacggtctgc tgcctgctct gaaaaagatc cacactaata
     6601 aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc catccgtttc
     6661 tggtgacatt tgttatcggc attatccttg cgatggagcg ttctaagcag gacgttaaca
     6721 gtattcagag caccaaaatt gccgtcggtg cgccgctcgg cgggattggc gatgcaatgt
     6781 tctggctaac gctactgccg atttgtggcg ggataggcgc aagccttgcg ctacaaggct
     6841 ctattcttgg cgcagtcgta tttattgtgc tgttcaacgt gatacacctg gggctgcgtt
     6901 ttggtctggc gcattatgct taccgcatgg gcgtggcggc gattccactc attaaagcga
     6961 ataccaaaaa agtcggccat gctgcgtcta tcgttgggat gacggtaatc ggcgcgctgg
     7021 tggcaaccta tgttcgttta agcaccacgc tggaaatcac agcgggcgac gcagtggtta
     7081 agttacagac tgatgttatt gacaaactga tgcctgcctt cttaccgctg gtctacacct
     7141 tgaccatgtt ctggctggta cgccgcggct ggagtccgct gcgcctgatt gcagttaccg
     7201 tggttctcgg catcgtcggt aaattctgcc atttccttta aatacaatga ggtattagat
     7261 gttaagtatt attttgacag ggcatggcgg gtttgccagt ggcatggaaa aagcaatgaa
     7321 gcaaatcctc ggcgagcaat cgcagtttat tgccatcgat tttcccgaaa cctcaagcac
     7381 cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg aagacggcat
     7441 tgtttttctg actgatttgc tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat
     7501 gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg caactgctac tggagatggt
     7561 gctggagcgc gaagggttaa gcggtgaaga attccgtgta caggcgctgg agtgtggaca
     7621 ccgagggctg accagtctgg tggacgagtt aggccgctgc catgaagaat gtccggtcga
     7681 ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga gggatggctc
     7741 gatgaccatc agttgcgtat tgctgacggt gtcatcgcag caatcgaacc gattccagtg
     7801 ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt acatcgacac ccatgtacac
     7861 ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg tactcgacaa gctggcaatg
     7921 cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca ccgtaaccgc gccgcttaat
     7981 accattcatg cggcgctgaa acgtattgct caacgttgcc aacgcggcgg acctggtgcg
     8041 caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat
     8101 ccgccggaat tgtttcgcga gcttgaaatt gccgagctgg atcaattgat tgccgtttct
     8161 cagcacacct tacgcgtggt agcgctggca ccggaaaaag agggggcatt gcaggccatc
     8221 cgccatctta aacagcaaaa tgtacgagtg atgctggggc atagcgcggc gacctggcaa
     8281 caaactcgcg ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta taacgggatg
     8341 acaggtttac atcaccgcga accgggaatg gttggcgcgg gattaacgga caagcgcgcc
     8401 tggctggaac tgatagccga tggtcatcat gtgcatccgg cggcaatgtc gctgtgttgt
     8461 tgctgtgcga aagagagaat cgtactgatc accgacgcga tgcaggcagc tgggatgccg
     8521 gatggtcgct atacgttatg tggtgaagaa gtgcagatgc acggtggcgt tgtccgtacc
     8581 gcgtctggtg ggctggcggg cagtacgctg tctgttgatg cggcagtgcg caatatggtc
     8641 gagttgacgg gcgtaacgcc tgcggaagcc atccatatgg cgtcgctgca tccggcgcga
     8701 atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc cagcgtcgtt
     8761 gcgctggata gcgggctaca tgtgcaacaa atctggattc agggtcaatt agcttcgttt
     8821 tgatggtttg ctccattatt gggccttcac ttcccccgta aggcctttct ttttctttct
     8881 ttttgatctg tgcagcggtg tcggatgcga cgctaacgcg tcttatgcga cctacggtac
     8941 gccgcatcca gcaatcggtg aacatagcga cgtcattacg caattcttta atcttccctt
     9001 gtcttttctt tcaatgatca cagatttcgt tttatttcct ttttctccat tgaactttcg
     9061 gtttcttttc tatagatttt aatcaacgaa agacatcacc aagtgaaatg aaacgaaagg
     9121 caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac taaggattga gttatgccag
     9181 aaaattacac ccctgctgcc gccgcaaccg gtacatggac tgaagaagag atccgccatc
     9241 agcctcgcgc atggatccgt tcactcacca acatcgacgc gctacgttct gcgctcaata
     9301 acttccttga accgttactg cgcaaagaga atctgcggat catcctgacc ggagccggaa
     9361 cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc cagccatacc ggtaaaaact
     9421 tcagcgccgt accgaccacc gatctggtca ccaatccgat ggactacctg aacccagctc
     9481 atccgctgct gttgatctcc ttcggtcgat ccggcaacag cccggaaagc gtcgctgccg
     9541 tggaactggc aaatcaattt gtaccagaat gctatcacct gccgatcacc tgcaacgaag
     9601 cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc gtttgccctg ctgatgcccg
     9661 cagaaacgca cgatcgcggc tttgcgatga ccagcagcat taccaccatg atggccagct
     9721 gcctcgcggt tttcgcacct gagacgatca acagccaaac cttccgcgac gtggcggatc
     9781 gttgccaggc gatcctgacc tcactgggcg atttcagcga aggtgtgttt ggttacgcac
     9841 cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca gggcgcagca cgcgagtcgg
     9901 cgctgaaagt gctggaactg actgcgggta aactggcggc cttctatgat tccccgaccg
     9961 gattccgtca tggcccgaaa tcactggtcg ataacgaaac gctggtggtg gtatttgtct
    10021 caagccaccc ttacacccgt cagtatgatc ttgatctgct ggctgaactc cgccgtgaca
    10081 accaggcaat gcgcgtaatc gccatcgccg cggaaagcac cgacatcgtc gctgccggtc
    10141 cacatattat cctgccgccg tcacgtcact ttatcgacgt tgagcaggca ttttgcttcc
    10201 tgatgtacgc ccagacgttt gcactgatgc agtcgctgca catgggcaat acgccggata
    10261 ccccatcagc cagcggcacc gttaaccgcg tggtgcaagg cgtaatcatt catccgtggc
    10321 aggcataaga ggatcgcatt atgagcatta tctccactaa atatctgtta caggacgccc
    10381 aggccaatgg ctacgcggtg cctgctttta acattcataa cgccgagacg atccaggcga
    10441 tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct cgccggaacg ccggggacct
    10501 ttaaacatat cgcgctggaa gagatctacg ccctgtgcag cgcctattcc acaacctaca
    10561 acatgccact ggcgctgcat ctcgaccacc acgaatcgct ggatgatatt cgccgtaaag
    10621 tccacgcagg tgtgcgcagc gcaatgatcg acggcagcca cttcccgttt gccgagaacg
    10681 tgaagctggt gaaatcggtt gttgacttct gccactcaca ggattgcagc gtggaagcag
    10741 aactgggccg cctgggcggt gttgaagatg acatgagcgt tgacgccgaa agtgcattcc
    10801 tgaccgatcc acaagaagct aaacgctttg tcgaactgac tggcgtcgac agcctggcgg
    10861 tagcgattgg tacggcgcac ggcttataca gcaaaacgcc gaagattgat ttccagcggc
    10921 tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct acatggtgcc agcgatgttc
    10981 cggatgaatt tgtccgtcgc actattgaac ttggcgtcac aaaagtgaac gttgccacag
    11041 aattaaaaat agccttcgct ggcgcggtta aagcctggtt tgcggaaaat ccgcagggta
    11101 atgatcctcg ttattatatg cgcgtcggaa tggatgcgat gaaagaagtt gtcagaaata
    11161 aaattaatgt ctgtggttca gcgaatcgaa tttcagcata atcattagtt ttttatgatt
    11221 tatcccaatg tacttcccga ttaatatcgg ggagtgcctt aatggaaaag gagatgacta
    11281 aaccttaata a
//
LOCUS       DAPPNJ010000147        11195 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000147 DAPPNJ010000000
VERSION     DAPPNJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.148"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..293
                     /locus_tag="RPM73_003292"
     CDS             <1..293
                     /locus_tag="RPM73_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDX6521626.1"
                     /translation="VIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAA
                     DITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(413..592)
                     /locus_tag="RPM73_003293"
     CDS             complement(413..592)
                     /locus_tag="RPM73_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDX6521627.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            666..2027
                     /gene="pabB"
                     /locus_tag="RPM73_003294"
     CDS             666..2027
                     /gene="pabB"
                     /locus_tag="RPM73_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDX6521628.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2031..2609
                     /locus_tag="RPM73_003295"
     CDS             2031..2609
                     /locus_tag="RPM73_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDX6521629.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2793..4157
                     /gene="sdaA"
                     /locus_tag="RPM73_003296"
     CDS             2793..4157
                     /gene="sdaA"
                     /locus_tag="RPM73_003296"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDX6521630.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4288..5886
                     /locus_tag="RPM73_003297"
     CDS             4288..5886
                     /locus_tag="RPM73_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX6521631.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKASPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5890..7446)
                     /gene="yoaE"
                     /locus_tag="RPM73_003298"
     CDS             complement(5890..7446)
                     /gene="yoaE"
                     /locus_tag="RPM73_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDX6521632.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEITTDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7909..8880
                     /gene="manX"
                     /locus_tag="RPM73_003299"
     CDS             7909..8880
                     /gene="manX"
                     /locus_tag="RPM73_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDX6521633.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8943..9743
                     /gene="manY"
                     /locus_tag="RPM73_003300"
     CDS             8943..9743
                     /gene="manY"
                     /locus_tag="RPM73_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDX6521634.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9756..10607
                     /gene="manZ"
                     /locus_tag="RPM73_003301"
     CDS             9756..10607
                     /gene="manZ"
                     /locus_tag="RPM73_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDX6521635.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10662..11120
                     /locus_tag="RPM73_003302"
     CDS             10662..11120
                     /locus_tag="RPM73_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDX6521636.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2727 a         2840 c         2835 g         2793 t
ORIGIN      
        1 ctgtaataga taaagccctg gatttcattg gtgccatgga tgtatcagcg ccaacaccaa
       61 gttcgatgaa tgaaagcaca gcgaagggaa tctttaaata tttaaaagaa ctgggagtac
      121 ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga aggctggaac cctgggttca
      181 cggaaaaaat ggttggatgg gcaaaaaaaa tggagtctgg tgaacgcact gtaattaaaa
      241 atcctgaata cttttcaaca tatatgcagg aagaactaaa agcactggtc tgagttaaat
      301 ttatatcagc ataaatgggt caggacgctt ttaatcacat ataaaaagcg tcctttttct
      361 cacaatcaaa tgatcatcgg cttctataac gaaatcgata cggattagtg tgttactcgt
      421 cttcatcctc aaaacgcgcc acgattcgct cgccggagtg gttggcacgc agctcttctg
      481 ccaccagcgc gattgcctgg ccgctgctca tcccttgtgc catcagttcc tggatccgct
      541 cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa acctgcaaac attgttaact
      601 cctgctaaat tgttggcgct aattatttca tgctacccgg cacatagcca gtagagtcag
      661 gactgatgaa gacgttatct cccgctgtga ttactttacc ctggcgtcag gacgccgctg
      721 aattttattt ctcccgctta agccacctgc cgtgggcgat gcttttacac tccggctatg
      781 ccgatcatcc gtatagccgc tttgatattg tggtcgccga tccgatttgc actttaacca
      841 ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc
      901 cgctacaggt gctccagcag gtgctggatc gcgcagacat tcgcccaacg cataacgaag
      961 atttgccatt tcagggcggc gcactggggt tgtttggcta cgatctgggc cgccgttttg
     1021 agtcactgcc agaaattgcg gaacaagata tcgttctgcc ggatatggca gtgggtatct
     1081 acgattgggc gctcattgtc gaccaccagc gtcatacagt ttctttgctg agtcataatg
     1141 atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt ctcgccgcag gaagatttca
     1201 cgctcacttc cgactggcaa tccaatatga cccgcgagca gtacggcgaa aaatttcgcc
     1261 aggtacagga atatctgcac agcggtgatt gctatcaggt gaatctcgcc cagcgttttc
     1321 atgcgaccta ttctggcgat gaatggcagg cattccttca gcttaatcag gccaaccgcg
     1381 cgccatttag cgctttttta cgtcttgaac agggtgcaat tttaagcctt tcgccagagc
     1441 ggtttattct ttgtgataat agtgaaatcc agacccgccc gattaaaggc acgctaccac
     1501 gcctgcccga tcctcaggaa gatagcaaac aagcagaaaa actggcgaac tcagcgaaag
     1561 atcgtgccga aaatctgatg attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg
     1621 tagccggttc ggtaaaagta ccagagctgt tcgtggtgga acccttccct gccgtgcatc
     1681 atctggtcag cactataacg gcgcgactac cagaacagtt acacgccagc gatctgctgc
     1741 gcgcagcttt tcctggtggc tcaataaccg gggctccgaa agtacgggct atggaaatta
     1801 tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg cagcattggc tatttgagct
     1861 tttgcggcaa catggatacc agcattacta tccgcacgct gactgccatt aacggacaaa
     1921 tatactgctc tgcgggcggt ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg
     1981 aaacttttga taaagttaat aagatattac gccaactgga gaagtaagac gtggaatacc
     2041 gtagcctgac gcttgatgat tttttatctc gctttcaact tttgcgcccg caaattaacc
     2101 gggaaaccct aaatcatcgt caggctgccg tgttaatccc catcgtccgt cgaccgcaac
     2161 cggggttgtt gctgactcag cgttcaattc atctgcgtaa acacgctgga caagtggcat
     2221 tccctggagg tgcagtcgat gacacggacg catcagttat cgccgccgcg ctgcgcgaag
     2281 ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg
     2341 tcgatagcgt aactggctat caggtaaccc cagtggtcgg cattatcccg cccgatctgc
     2401 cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga aatgccgctc gcccaggcat
     2461 tacatctggg tcgttatcac cctttagata tctaccgccg tggcgattca catcgggtat
     2521 ggctgtcctg gtacgaacag tattttgtat ggggaatgac cgcaggcata attcgtgagc
     2581 tggcgctgca aattggtgtg aaaccctgac tatacttatc tttacatcta caaaacacta
     2641 cttgagacaa tcatcgcaat attagttaaa tcgcggtttt tgattagttt aattcatgtg
     2701 aatagttaag ccagtcgccg cgttccctct tacactatgc gctgttatta gttcgttact
     2761 ggaagtccag tcaccttgtc aggagtatta tcgtgattag tctattcgac atgtttaagg
     2821 tggggattgg tccctcatct tcccataccg tagggcctat gaaggcgggt aaacagttcg
     2881 tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac tcgcgttgcc gtggacgttt
     2941 atggttcact gtcgctgacg ggtaaaggcc accacaccga tatcgccatt attatgggtc
     3001 ttgcaggtaa cgaacctgcc accgtggata tcgacagtat tcccggtttt attcgcgacg
     3061 tagaagagcg cgaacgtctg ctgctggcac agggacggca tgaagtggat ttcccgcgcg
     3121 acaacgggat gcgttttcat aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc
     3181 acgcctataa cggcgatgaa gtcgtctaca gcaaaactta ttattccatc ggcggcggtt
     3241 ttatcgtcga tgaagaacac tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc
     3301 cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga aaccggctat tcgctgtctg
     3361 gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa agagatcgac gagtatttcg
     3421 cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac
     3481 tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca
     3541 gcgataaact gtctaacgat ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg
     3601 cagttaacga agaaaacgcc gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct
     3661 gcggtatcgt tccggcagtg ctggcttact atgaccactt tattgaatcg gtcagcccgg
     3721 acatctatac ccgttacttt atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg
     3781 cctctatttc cggtgcggaa gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg
     3841 ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg
     3901 aaattggcat ggaacacaac cttggtttaa cctgcgaccc ggttgcaggg caggttcagg
     3961 tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc gattaacgcc gcacggatgg
     4021 ctctgcgccg caccagtgca ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg
     4081 aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc acgcggtggt ctggcaatca
     4141 aagtccagtg tgactaatac ttcttactcg cccatctgca acggatgggc gaatttatac
     4201 ccgctttctc gtctgctgta atattcccca ctacacttcc actgttgcgt caggcgtttg
     4261 tcgccatacg cttacagggt ggcccgcatg caaaaagcac aacggatcat taaaacctat
     4321 cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg
     4381 agcgtgcgat ttatttcaca gcgtaactta aatcaacaac gggtagtaca attcgccaat
     4441 cacgctgtag aggaattaga taaagtactg cttcccctac aggcaggtag cgaagtcttg
     4501 cttccgctga ttggtctgcc ctgctctgtc gcccatttgc cattacgtaa acaggcggca
     4561 aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg gcacactcta ttgctccagc
     4621 atttttggtt atcgcaatgt gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa
     4681 ccacttttac gcctgacgat cgaccgtgcc ctgattaaag ccagtccggt tttgattcaa
     4741 tggacgcctg cagcgggcag tagcaatgct ggggtcatgg agatgattaa catcgactta
     4801 ctgacggcaa tgctccttga gccacaactg ccgcaaatca gtagcgccag cctgacggtg
     4861 gacaaacggc atttgctcta tggtaatggg ctggtagatt cccttccgca acctgaagac
     4921 aatgaaaact atcaggtttc ttcgcaacgc tttcctttta ccattaacgt taatggtccg
     4981 ggggctacgg cgctggcatg gcactatctt ccaacacaat taccgctggc ggtgctgcta
     5041 agtttactgg tgggctacat cgcctggctg gcgaccgctt accggatgag cttttcccgc
     5101 gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat
     5161 gcgcgcagcc agcaatgtat tggtgtagag attttgctgc gctggaacaa tccgcgtcag
     5221 ggctggattt caccggatgt gtttattcct atcgcggaag aacatcattt aattgtgcca
     5281 ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc atgttttccc gatgagtagt
     5341 cagtttcatg ttggcattaa cgtcgcaccc agccattttc gccgtggtgt gctgataaaa
     5401 gatctcaatc agtactggtt tagcgctcac ccgattcagc aactgatcct cgaaatcacc
     5461 gaacgcgatg ccttactgga tgttgattat cggattgccc gcgagctaca tcgtaaaaac
     5521 gtcaaactgg cgattgatga cttcggcacc ggcaacagtt cattttcctg gcttgaaaca
     5581 ttacgtcctg acgtgctgaa aattgataag tcatttaccg cagctatagg ttctgacgcg
     5641 gttaactcga cggtgaccga tatcatcatc gcgctggggc aaagactgaa tattgaactg
     5701 gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat
     5761 attttgcaag ggtatttgta cgcacagccg atgccgctac gtgattttcc caaatggctg
     5821 gcgggcagcc aaccgccgcc cgcccggcat aatggacata tcacgcccat tatgccgtta
     5881 cgttaacgct tactcatctt catcgtgcgc cggttgctct ttaacaatgc gaaccagatc
     5941 aacacgataa tcattggctt caatgatggt gatatgcagt ggccctacat caatcacatc
     6001 gcccacacgg ggaatgtgac catttgccga gatcaccagg cccgcgaccg tcgcgatatc
     6061 atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc aaggcatgca aatctgtacc
     6121 gccttttacc agccagccgt caccatcggt agtgatttcc ggcgtttcgt cagcgtccgg
     6181 gaattcaccc gcaatggctt ccagcacatc cagcggcgtg accaagcctt gtaccacacc
     6241 aaactcgttg gtcacgataa caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt
     6301 gatcggatcg agggtttccg ggacgataat cgccggagac gccgaagcaa tcgccgccac
     6361 atcaacgccc tcttccagcg ccaccagcag ttctttagca cgtacgattc cgatgatttc
     6421 atccagttca ccgcgacata ccgggaacag gctgtgcggt gaggagagca gctgctcgcg
     6481 gatttcatcg accccgagat tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat
     6541 cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta atcatgtaac gttcttcttc
     6601 ggcaaatgca ccttccggga tcggcatcgg catcgggtta tcggcatcgt gctgaacatt
     6661 ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg
     6721 caaagtcgac tggtgacgaa taaagttgcg acgcgcaatc tggttaaaca cttcgatgat
     6781 gatcgagaag ccaatcgcgg catacaggta acctttcgga atgtggaaac cgaaaccttc
     6841 tgccaccaga ctcagaccaa tcattaacag gaagctcaga cagagcacca ccaccgtggg
     6901 gtgctggtta acgaatcgcg tcagcggttt ggatgccagc aacataaccg ccatcgcaat
     6961 cactaccgcc gccatcatca ccggcagatg gttaaccatc cctactgcag taattaccgc
     7021 atccaacgag aagacggcgt caaggatgac aatctgtgtg acgaccaccc agaaactggc
     7081 gtagccttta ccgtggccgg aatcatgatc gcggttttcc agccgttcat gcagttcggt
     7141 tgttgctttg aacagcaaga atatcccccc gaacaacata atcaggtcgc gtccggagaa
     7201 ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg accatccatg aaatcagcga
     7261 cagcagcccc agacgcataa tcagcgccag tgataacccc agcaaacgcg ctttatcgcg
     7321 ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag accaggttat cgatacccag
     7381 cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa attgaggggt ccattaagaa
     7441 ttccatgaca agctcctgct taaggaatag ctattcgacg ccagaaataa tgcaggcgta
     7501 acgacaaaat gcaaacgaaa ggtgcggcat agagagccag aaaggtaggc gttaaaaggc
     7561 ctgatgctga aatgacgtcg gtgacgatcc atactgcggg ctactgccct atactccatg
     7621 gttgttaaac gggagttaaa catatcagag acgcctctga tttggcaaag atttaccttc
     7681 ctttgcaaac gaatgtgaca aggatatttt acctttcgaa atttctgcta atcgaaagtt
     7741 aaattacgga tcttcatcac ataaaataat tttttcgata tctaaaataa atcgcgaaac
     7801 gcaggggttt ttggttgtag cccttatctg aatcgattcg attgtggacg acgattcaaa
     7861 aatacatctg gcacgttgag gtgttaacga taataaagga ggtagcaagt gaccattgct
     7921 attgttatag gcacacatgg ttgggctgca gagcagttgc ttaaaacggc agaaatgctg
     7981 ttaggcgagc aggaaaacgt cggctggatc gatttcgttc caggtgaaaa tgccgaaacg
     8041 ctgattgaaa agtacaacgc tcagttggca aaactcgaca ccactaaagg cgtgctgttt
     8101 ctcgttgata catggggagg cagcccgttc aatgctgcca gccgcattgt cgtcgacaaa
     8161 gagcattatg aagtcattgc aggcgttaac attccaatgc tcgtggaaac gttaatggcc
     8221 cgtgatgatg acccaagctt tgatgaactg gtggcactgg cagtagaaac aggccgtgaa
     8281 ggcgtgaaag ctctgaaagc caaaccggtt gaaaaagccg cgccagcacc cgctgccgca
     8341 gcaccaaaag cggctccaac tccggcaaaa ccaatggggc caaacgacta catggttatt
     8401 ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg tcgccacccg ctggaccaaa
     8461 gaaaccaatg tctcccgtat tattgttgtt agtgatgaag tggctgcgga taccgttcgt
     8521 aagacactgc tcacccaggt tgcacctccg ggcgtaacag cacacgtagt tgatgttgcc
     8581 aaaatgattc gcgtctacaa caacccgaaa tatgctggcg aacgcgtaat gctgttattt
     8641 accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg tgaaaatcac ctctgttaac
     8701 gtcggtggta tggcattccg tcagggtaaa acccaggtga ataacgcggt ttcggttgat
     8761 gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg gtattgagct ggaagtccgt
     8821 aaggtttcca ccgatccgaa actgaaaatg atggatctga tcagcaaaat cgataagtaa
     8881 cgtattgtgt tgattatcac tcagttttca cacttaagtc ttacgtaaac aggagaagta
     8941 caatggagat taccactctt caaattgtgc tggtatttat cgtagcctgt atcgcaggta
     9001 tgggatcaat cctcgatgaa tttcagtttc accgtccgct aatcgcgtgt accctggtgg
     9061 gtatcgttct tggggatatg aaaaccggta ttattatcgg tggtacgctg gaaatgatcg
     9121 cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga cgccgctctg gcttctatca
     9181 tttctaccat tctggttatc gcaggtcatc agagcattgg tgcaggtatc gcactggcaa
     9241 tccctctggc cgctgcgggc caggtactga ccatcatcgt tcgtactatt accgttgctt
     9301 tccagcacgc tgcggataag gctgctgata acggcaacct gacagcgatt tcctggatcc
     9361 acgtttcttc tctgttcctg caagcaatgc gtgtggctat tccggccgtc atcgttgcgc
     9421 tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc gattccggaa gtggtgacca
     9481 atggtctgaa tatcgccggt ggcatgatcg tggtggttgg ttatgcgatg gttatcaaca
     9541 tgatgcgtgc tggctacctg atgccgttct tctacctcgg cttcgtaacc gcagcattca
     9601 ccaactttaa cctggttgct ctgggtgtga ttggtactgt tatggcagtg ctctacatcc
     9661 aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc tcaggcagct ggtaacaacg
     9721 atctcgataa cgaactggac taacaggtga gcgaaatggt tgatacaact caaactacca
     9781 ccgagaaaaa actcactcaa agtgatattc gtggcgtctt cctgcgttct aacctcttcc
     9841 agggttcatg gaacttcgaa cgtatgcagg cactgggttt ctgcttctct atggtaccgg
     9901 caattcgtcg cctctaccct gagaacaacg aagctcgtaa acaagctatt cgccgtcacc
     9961 tggagttctt taacacccag ccgttcgtgg ctgcgccgat tctcggcgta accctggcgc
    10021 tggaagaaca gcgtgctaat ggcgcagaga tcgacgacgg tgctatcaac ggtatcaaag
    10081 tcggtttgat ggggccgctg gctggtgtag gcgacccgat cttctgggga accgtacgtc
    10141 cggtatttgc agcactgggt gccggtatcg cgatgagcgg cagcctgtta ggtccgctgc
    10201 tgttcttcat cctgtttaac ctggtgcgcc tggcaacccg ttactacggc gtagcgtatg
    10261 gttactccaa aggtatcgat atcgttaaag atatgggtgg tggcttcctg caaaaactga
    10321 cggaaggggc gtctatcctc ggcctgtttg tcatgggggc attggttaac aagtggacac
    10381 atgtcaacat cccgctggtt gtctctcgca ttactgacca gacgggcaaa gaacacgtta
    10441 ctactgtcca gactattctg gaccagttaa tgccaggcct ggtaccactg ctgctgacct
    10501 ttgcttgtat gtggctgctg cgcaaaaaag ttaacccgct gtggatcatc gttggcttct
    10561 tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg actgtaagac tgttgtacac
    10621 taccggggcc ttttggcccc gtttttttat ctggaggatt aatgacaatc acggacctgg
    10681 tactgattct tttcatcgcc gcactcctgg ccttcgcgat ctacgatcag ttcatcatgc
    10741 cccgccgtaa cggccccacc ctgctggcaa ttcctttgct ccggcgtggt cgcatcgata
    10801 gcgttatctt cgtcggattg attgtcattc ttatctataa caacgtcacg aatcatggcg
    10861 cgctaataac aacatggtta ttaagtgcac tggctctgat gggtttttat atattctgga
    10921 tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt cttcgccaat gtctggattg
    10981 aatatagccg aatcaaagcg atgaacttgt cggaagatgg cgtgttggtg atgcaattag
    11041 aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga tctggaaaaa atttataagc
    11101 ttctcgtatc aactcaataa cttatgaatt tagccaaagc tatgtttagt gtatttttaa
    11161 taatcagaca tagcttaggc tatattatct cttcc
//
LOCUS       DAPPNJ010000148        10991 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000148 DAPPNJ010000000
VERSION     DAPPNJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.149"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(58..1887)
                     /gene="glmS"
                     /locus_tag="RPM73_003303"
     CDS             complement(58..1887)
                     /gene="glmS"
                     /locus_tag="RPM73_003303"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDX6521637.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="RPM73_003304"
     CDS             complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="RPM73_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDX6521638.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3770..4189)
                     /gene="atpC"
                     /locus_tag="RPM73_003305"
     CDS             complement(3770..4189)
                     /gene="atpC"
                     /locus_tag="RPM73_003305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDX6521639.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="RPM73_003306"
     CDS             complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="RPM73_003306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDX6521640.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="RPM73_003307"
     CDS             complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="RPM73_003307"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDX6521641.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="RPM73_003308"
     CDS             complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="RPM73_003308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDX6521642.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="RPM73_003309"
     CDS             complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="RPM73_003309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDX6521643.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="RPM73_003310"
     CDS             complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="RPM73_003310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDX6521644.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="RPM73_003311"
     CDS             complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="RPM73_003311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDX6521645.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="RPM73_003312"
     CDS             complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="RPM73_003312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDX6521646.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="RPM73_003313"
     CDS             complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="RPM73_003313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDX6521647.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2613 a         3006 c         2733 g         2639 t
ORIGIN      
        1 atgtcagtta taaaaaaggg ccaccggtcg gcagcccttt tttatctatg atagtgatta
       61 ctcaaccgta accgattttg ccaggttacg cggctggtca acgtcggtgc ctttgatcag
      121 cgcgacatgg taagccagca gctgcagcgg aacggtgtag aagatcggtg caatcacctc
      181 ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac ccgcatcctg
      241 atcggcgaag acatacaact gaccgccacg cgcgcgaact tcttcaatgt tggatttcag
      301 tttttccaac agttcgttgt tcggtgcaac gacgataacc ggcatatccg cgtcaatcag
      361 cgccagcggg ccgtgtttca gctcgcctgc agcgtaggct tcagcgtgaa tgtaagagat
      421 ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac ggcccaggaa
      481 cagcgcgtga tgtttgtcag agaaatcttc tgccagagct tcaatgcgtt tgtcctgaga
      541 cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt catgttcaat
      601 ggaggcatcc agacctttca ggcgagacag cttcgccacc agcatcaaca gcacagttaa
      661 ctgagtggtg aatgctttag tggatgccac gccgatttcc gtacccgcgt tggtcatcag
      721 cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg ccagcgaacc
      781 aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg tttcgccaga
      841 ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttgcgat agcggaattc
      901 agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt aacgggaaac
      961 cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa ccttcgacag
     1021 cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc tgatgcgccc
     1081 ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc tctttctgca tgtagtgacg
     1141 gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct gacgttttac
     1201 ttccgcgcca gttttatcga agatgtttac cgaacggcga gtaatttccg caatatcgcc
     1261 ctcttcaagg aagataaagc gacgggtcac cggcaacagc gccagctggt cagaagcgat
     1321 aaagttttcg cccataccca ggccaatcac cagcggacta ccagaacgtg ccgccagcag
     1381 ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccacgca gttgcgggat
     1441 agcacgcaga acagcctcac gcagagtacc gccctgtttc agctcccagt tcaccagatg
     1501 ggcaattact tcggtgtctg tttcagaaac gaaggtatag ccacgcgctt ttaactcttc
     1561 gcgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt gttcagaaac
     1621 atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag tatgagcaat
     1681 acccgtaccg ccatgcagag gatgttcttc cgctgcctga gccagcatct ggactttacc
     1741 gaggcgacgc aggcgggtca tatgaccttc cgcatcaaca acggccagac cggcagagtc
     1801 atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta catcacgttg
     1861 cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc gttgtcggtc
     1921 aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg ttatttttat
     1981 agttactgct tgtgggaggg agattatgtt atctcctcat cccagatgac cggattaccc
     2041 ggccagaatt actttttctt taccggacga cgccagcctt ctttctgagt ctgcggcaca
     2101 cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc cgcagcaatg
     2161 gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc gacaaacaca
     2221 tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt aatggttccc
     2281 gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag atgaccagct
     2341 ttcgagcctt tacccagacg cgcttttttc atctcaacaa agttaccgac gtgtgcacct
     2401 tccagcaact cagcaccagg acgcagacgg gcaaacgggc caatagtaca ggccgctgcc
     2461 agattcgcgt cttccacaac ggtatacgga ctgatttcgc aatcatcgcc aatcacgctg
     2521 tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac gttgccctcg
     2581 atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc gcgcagatca
     2641 aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc ctgttcggac
     2701 tggtaaacgc gctccagacg ggagagttgc aggcggttat tcacaccttc tacttcgctt
     2761 aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag cgcaataatg
     2821 tcggtgatgt agtattcacc ctgagcgtta ttgttggtca gcttcgccag ccagcgtttc
     2881 atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg acgctgctcg
     2941 tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cattttcacg ggtgatacgt
     3001 ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc ctgcggttta
     3061 gcatcacgca ggcgctggag tgtttcgaca gagatcagcg gcacgtcgcc gtagagcatt
     3121 aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcattgcatg acccgtaccc
     3181 agctgctctg cctgaagcac ccagttaagg ttgtcgtctt tcagcgcctg tttaagcaga
     3241 tcgccgccgt gaccgtacac caggtgaacg tgcgctgcgc ctaattcatt cgcagcatca
     3301 atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg aagatcggaa
     3361 tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc tcatagcatt attcaacata
     3421 cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa aaactacatt
     3481 tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc cccgctgacg
     3541 aaagtgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag gcagtacctt
     3601 catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa acgggaaaac
     3661 aggggtgttt tatatcagca ggatctatgt gaacgctctt caggacggat cacgcgtaaa
     3721 aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt tacatcgctt
     3781 ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc gcagacgcct
     3841 gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta cgtttcgctt
     3901 ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc agaacggtca
     3961 cgttgccagg ctgcacttca agaatgccgc cagacagata gataaactct tcgtgaccgt
     4021 gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt gcgtggccag
     4081 ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc agaccagaga
     4141 acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata tcaccctccg
     4201 attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac cgaccatgta
     4261 gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa agccacggat
     4321 ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg ccacgaagaa
     4381 cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt tgtcttcttc
     4441 agacagttca tccataccca ggatggcgat gatgtctttc agttcctgat aacgttgcag
     4501 gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca gcgggtccag
     4561 ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag acgcgatctg
     4621 acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag acgggtcagt
     4681 caagtcatcc gcaggtacgt ataccgcctg tacggaggtg atagaaccag ttttggtgga
     4741 ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat aacctaccgc
     4801 tgaaggcata cggcccagca gtgcggatac ttccgtaccg gccagggtgt aacgatagat
     4861 gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag ccatggtcag
     4921 accggtcaga gcaacgcgca gacggtttcc cggcggctcg ttcatctggc catacaccag
     4981 ggatactttg tcgataacgt tggagtcggt catttcgtgg tagaagtcgt taccctcacg
     5041 agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg cgatgttacg
     5101 aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca gaccaacttt
     5161 accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt ccagcagttc
     5221 ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc aacgctcttc
     5281 ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga tacggcccag
     5341 agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat ccagaccgcg
     5401 acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccgc cgagctgctg
     5461 ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat cgtacacgcg
     5521 cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct ggacaatctt
     5581 tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc ggcccccgag
     5641 acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta taccaactgc
     5701 agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac catacgggcg
     5761 gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc gacataacga
     5821 cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc ccaggattta
     5881 tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat ggtcggaacc
     5941 tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag acggccttcg
     6001 tcgtaggcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga agggttatcc
     6061 cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa cgacacgcct
     6121 ttcgagccga tcattgcgag gtcgcattga acgcctttgt cggtccaggt cttcatttcc
     6181 gccagcagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg gtcggtcgac
     6241 accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg cttatattcc
     6301 agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata aggacggctg
     6361 gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc catcgcttta
     6421 gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt tgcgccggcc
     6481 atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga cgttaccagg
     6541 attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt tcgtcgttgt
     6601 agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg acgtaagcca
     6661 gcagagcggc ttcgaagctg ccaattttcg acagttcaac atccgccagg taaccacgtt
     6721 ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac tgtttctgtt
     6781 tcagcagttc ggtcactttc tgaccgtggt caagctgctt acgtgttgca tcgtcaaggt
     6841 cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg gtacggatac
     6901 caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg gatacggaaa
     6961 tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc aggaagatct
     7021 gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca cccgcctgag
     7081 tttcgataat cggcagtgcg gtcagagaac cggttttccc tttcacttca cctttggtga
     7141 aggcttcaac gtattcggcg ttaacacgtg cagcacgctc cagcagacga gagtggaggt
     7201 agaaaacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc agggagatct
     7261 gacggtaagc aacagcctgt ttagacaggt catcgtaaat gatcagcgca tcttcaccgc
     7321 ggtcacggaa gtattcgccc attgcgcaac cggcatacgg tgccaggtat tgcagtgcag
     7381 cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg tgctcttcca
     7441 gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca tagatacatt
     7501 tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt ttacctgtct
     7561 gacggtcacc gatgatcaat tcacgctgac cacgaccgat tgggatcatg gagtcaacgg
     7621 ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg cccggagcga
     7681 ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg attggtgcac
     7741 ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc aggatacggc
     7801 cagtacactt aactttcatg ccttcggcaa ggtcagcgta cggacccata acaaccgcac
     7861 ctacagagtc gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc agggagatca
     7921 tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca cttacagaaa
     7981 caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg cgctgcttga
     8041 tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa gactgcaaga
     8101 cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata tcacccgctc
     8161 ggatgataac gcctgccatt acagacttat cgattttgca attcagctta actttgcgtg
     8221 acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc agtgcggcag
     8281 cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga ataaactgct
     8341 ccagaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga atcaggttct
     8401 gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggcg agcgtttctg
     8461 gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc tcggcggcaa
     8521 acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag tcaaaagctg
     8581 ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc ctccttacag
     8641 ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg aacgttcgat
     8701 gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct cttcacgggc
     8761 acgtttacgc tcggcttcaa tttctgcctg cgcctgggcc acgattttag tacgttcctg
     8821 ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg cctgctcgat
     8881 gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg cctttgcaag
     8941 gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt ctttttgacg
     9001 tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca gaacgaacag
     9061 gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac aatgcctcta
     9121 tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac gcgacagcga
     9181 acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc agacccataa
     9241 cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct gcgccttcca
     9301 ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc agacccatca
     9361 tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca gtctccagtt
     9421 tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc catcgacaga
     9481 tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag gatgtggaaa
     9541 atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc agcaatcaga
     9601 atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccaag tgaaactggt
     9661 ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc ccagtgattg
     9721 aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt gatgctgtag
     9781 aacagaatca ggataaatac gcccaatgcc atagacagcg ttacgttcac gtccgcagac
     9841 ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg cagcaggtcg
     9901 ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt cagggccagc
     9961 ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt aacaaagccg
    10021 atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc ctttttggct
    10081 acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa catggagtca
    10141 atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga gaatgtacgc
    10201 aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt catattttct
    10261 gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt atctgaacca
    10321 ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa accgccaacg
    10381 ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcgccaaat gcgaatgtcc
    10441 aggccacccg gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat atcataaaca
    10501 aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc cagaaggggt
    10561 ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg agcagaagct
    10621 tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact cctgctccct
    10681 tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa gcatcaaaaa
    10741 gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca aaaggtgaat
    10801 aaatgtttaa atatttttcc agtgcataca attgcgactt ttctgctaac cctgttcgat
    10861 catgaaaaac tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca gatcacatat
    10921 tgcgcatgtt cgcgcacagc atatttattt acttggcaaa tgatgccttt gcaagtttat
    10981 gatatttcag t
//
LOCUS       DAPPNJ010000149        10950 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000149 DAPPNJ010000000
VERSION     DAPPNJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.150"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            25..648
                     /gene="nfeR"
                     /locus_tag="RPM73_003314"
                     /gene_synonym="yqjI"
     CDS             25..648
                     /gene="nfeR"
                     /locus_tag="RPM73_003314"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDX6521648.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(802..2322)
                     /gene="aer"
                     /locus_tag="RPM73_003315"
     CDS             complement(802..2322)
                     /gene="aer"
                     /locus_tag="RPM73_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDX6521649.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2740..4119
                     /gene="ygjG"
                     /locus_tag="RPM73_003316"
     CDS             2740..4119
                     /gene="ygjG"
                     /locus_tag="RPM73_003316"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDX6521650.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4161..4493)
                     /locus_tag="RPM73_003317"
     CDS             complement(4161..4493)
                     /locus_tag="RPM73_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDX6521651.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4712..5695
                     /gene="ebgR"
                     /locus_tag="RPM73_003318"
     CDS             4712..5695
                     /gene="ebgR"
                     /locus_tag="RPM73_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDX6521652.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5879..8971
                     /gene="ebgA"
                     /locus_tag="RPM73_003319"
     CDS             5879..8971
                     /gene="ebgA"
                     /locus_tag="RPM73_003319"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDX6521653.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            8968..9417
                     /locus_tag="RPM73_003320"
     CDS             8968..9417
                     /locus_tag="RPM73_003320"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDX6521654.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9480..10913
                     /locus_tag="RPM73_003321"
     CDS             9480..10913
                     /locus_tag="RPM73_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6521655.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTSGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         2612 a         2948 c         2778 g         2612 t
ORIGIN      
        1 ctaatcaagc aaaaggaggc tgatatgagc catcatcacg aagggtgttg taaacatgaa
       61 ggccagccac gccatgaggg ctgctgcaaa ggtgagaagt cagaacacga gcactgcgga
      121 cacggtcacc agcatgaaca cggtcaatgc tgcggtggtc gccacggtcg cggcggcggt
      181 cgtcggcaac gtttctttgg tcacggtgaa ttgcgtctgg tgattctgga tattctctcg
      241 cgcgatgaca gccacggtta cgaattgatt aaagcgattg agaacctaac ccaggggaat
      301 tacaccccaa gcccgggcgt catctaccca acgctggatt ttctgcagga gcagtcgctg
      361 attaccatcc gcgaagagga aggtggtaag aagcagattg cgctgaccga acaaggcgcg
      421 cagtggctgg aagaaaaccg cgaacaggtg gagatgattg aagaacgcat caaagcgcgt
      481 tgcgttggcg cggcgctgcg ccagaacccg caaatgaagc gggcgctgga taattttaaa
      541 gcggtgctgg atttacgcgt caaccagagc gatatcagtg atgcacaaat aaaaaagatc
      601 attgcggtga tcgaccgcgc cgcttttgat attacgcaac tggattaatc gccgcatccg
      661 ccagtggcgc ggtgcaattg ccggatgcga cgcttgacgc gccttatccg gcctacaccc
      721 gctacacacc ccgcaggcct gataagatgc gccagcatcg catcaggcat tgtgctccaa
      781 ccgccggatc cggcataccg attaatgcag caccgtcacc gcgtcttcca gtcggctggc
      841 gcggtgtttc accatcgccg acacctgcgc actctcttcc accagctcgg catttttctg
      901 ggtgatcagg ttaagctcat ccactgcacg ggtcaggctg gaaagcccat cggcctgttc
      961 cagcgttgaa tggctaatct gggcgatcaa ctgggtgacg tttttcacct gtgccacaat
     1021 atcttccatc gtccgtccgg cggcgtgtac ctgctgcgaa ccggattgca ccttatcagc
     1081 actggcatca atcagcttgc gaatatcgtt ggcagcatta gcgctgcggc tggctaaatg
     1141 acgcacttcc cctgccacca ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc
     1201 caccgccgca ttcagggcca gaatattggt ctgaaacgca atatcgttaa tcagcgaagt
     1261 aatggtgcca atgcgctggg tactgtcggc gatatcgtcc attgtcttga tcaccgtggt
     1321 catcgcctcc ccaccctgca ctgccgcatt actggcggtg atagaaagtt tatcggcagc
     1381 cgacgccgtg gcagagttct gtttcaccga cgccgccatt tggttcatgg tcgccactgt
     1441 ttgctgaacg ttatcaactg tctgctgggt atgttcgttc agttcatcgg tgcctttcgc
     1501 cagcgtctca ctgccatttc tgacactgga cacctggctt gagacatcgt taattagcca
     1561 acggcacatc aggccaagct gccctaccgc gcgtaatgtc agccccagct catcgctgcg
     1621 attcaggtgc tcaacactat tacgctctcc ggtcgccacc ttcagtgcct gacgggcgac
     1681 attttctatc gggcgcacaa tctgccattc aaaacaagcg cttgccaaca ataccactaa
     1741 cacacagagg aaatacgtca ccaccggggc agcaacaaac caaagcatgg ccgccagcaa
     1801 gataaacatc agggtcatca ctccacgcgc ccgccagcga agcggtaatg aaggcagttt
     1861 acccagccag cctttacgca ccaccaggcc tttatgaata cgcttactgg tacgtccggc
     1921 gttcagcgct ttgtacagcg gctccaccgc cgcaatctct tcatccgtcg cccgggtacg
     1981 aatcgacata tagccactga tttttccctc gcgcaccatc ggtaccgcat tggcccgcac
     2041 ccaataatgg tcgccatttt tgcggcgatt tttcacgatg ccgctccagg gctccccttt
     2101 tttcagggtg aaccacatat ccgcaaacgc cgcttttggc atatccgggt gacgcaccat
     2161 gttgtgcggc tgcccttgta actcttgcaa ggtaaagccg ctcacctgca caaaagtgtc
     2221 attagcatga gtgatatagc tttgcagatc ggtagtggac atcagagtgg tatcgtccgc
     2281 cagcggggta ttttgctggg tgacatacgg atgagaagac atgttcgcgt cctgtgcagg
     2341 ttatatggtt gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac
     2401 cgattaattt gatttagatc gcaatttgcg attaaagcac aaatctaatt ctttgattta
     2461 aaatactttc actctgttac tatacgaaaa cgttaattat cttgcccaaa aatcaggcaa
     2521 ttattgccct gaaaacgtgc atttgcgcag caatcatcaa atccataccc gacaaaaacc
     2581 gtgcaaaata acaacaaatg ttaacagata gcattaaata ttgcacaaat gataaccgaa
     2641 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     2701 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     2761 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     2821 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     2881 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     2941 tggcaagcgg gaggtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     3001 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     3061 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     3121 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     3181 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     3241 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     3301 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     3361 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     3421 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     3481 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     3541 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     3601 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     3661 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     3721 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     3781 aatgtgttgc tggagcagaa cttaccggct caggctgagc aaaaaggcga tatgttgctg
     3841 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     3901 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     3961 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     4021 gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     4081 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     4141 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     4201 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     4261 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     4321 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     4381 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     4441 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     4501 tcccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     4561 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     4621 agaaacgcgc tttatttact gaaaacaggt gacccgataa gcacttcctc tacaatgggg
     4681 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     4741 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     4801 gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     4861 tagtgcccgt aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     4921 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     4981 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     5041 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg ccctgcgcgc
     5101 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     5161 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     5221 caaccagggc gttaatcgta ttggttttat tggcggtgaa gatgagcctg gcaaggcgga
     5281 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     5341 tatctggcgc ggcggttttt ccagttcgtc gggttatgaa ctggcaaaac aaatgctggc
     5401 gcgggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     5461 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     5521 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     5581 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     5641 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     5701 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     5761 agttaaagta aatgcattta cctgctactt tttagtaaaa attttactaa actccccagc
     5821 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     5881 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     5941 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     6001 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     6061 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     6121 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     6181 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     6241 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     6301 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     6361 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     6421 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     6481 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     6541 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc
     6601 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     6661 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     6721 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     6781 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     6841 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     6901 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     6961 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     7021 gcgtttttac gaactgtgtg atatctacgg cttgtttgtg atggcggaaa ccgacgtcga
     7081 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     7141 aaaagtctac gtcgagcgca ttgttcgcca tattcacgcg cagaaaaacc atccgtcgat
     7201 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     7261 cgcagcgaag gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     7321 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     7381 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     7441 aggcgggctg acggagtacc agaacgtctt ctataagcac gattgtattc agggacatta
     7501 tgtctgggag tggtgcgacc acgggatcca ggcgcaggat gacaacggca acgtctggta
     7561 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     7621 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     7681 ggtaaaaatc cacgcgctgg atctgactcg cggcgagctg aaagtcgaaa ataaactgtg
     7741 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgtcgaag gtgaaacgct
     7801 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     7861 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     7921 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     7981 aaacaccgcg caaccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     8041 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     8101 tggcaaaccg acatcctggc aggtgaatgg cgagtcgctg ctgacccgcg agccaaagat
     8161 caacttcttc aagccaatga tcgacaacca caagcaggag tacgaagggc tgtggcaacc
     8221 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
     8281 tgaagtgttg atcatcagcc gcacagttat tgccccgccg gtgtttgact tcgggatgcg
     8341 ctgcacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
     8401 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
     8461 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
     8521 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
     8581 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
     8641 cggcaacggt ttgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
     8701 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
     8761 cctgaacctc gatcaccagc tgcttggcct cggctccaac tcctggggca gcgaggtgct
     8821 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
     8881 tggcggagaa gctaccgcgc aaagcctggc gtcgtatgag ttcggcgcag ggttcttttc
     8941 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
     9001 cagatttacg cctctggcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
     9061 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
     9121 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
     9181 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
     9241 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
     9301 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
     9361 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
     9421 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
     9481 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgccg
     9541 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
     9601 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
     9661 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
     9721 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
     9781 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
     9841 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
     9901 tttccaccag cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
     9961 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    10021 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    10081 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    10141 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    10201 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    10261 aatttaccgg cggatcggtg caggtattcc acggcatggc ggcgtatttt ggtctaccgg
    10321 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    10381 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    10441 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    10501 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    10561 tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    10621 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    10681 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    10741 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    10801 gcggtattgt tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat
    10861 taacggctga agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat
    10921 aataaaaaat gagcaggcat tttccctctt
//
LOCUS       DAPPNJ010000150        10943 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000150 DAPPNJ010000000
VERSION     DAPPNJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.151"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            24..392
                     /locus_tag="RPM73_003322"
     CDS             24..392
                     /locus_tag="RPM73_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521656.1"
                     /translation="MAALIEHLELINPSGRLSQNTRSAMLQLREEEWSEFFFWLLNSL
                     ECLDYVIINLTPESKKTLMSEHRNNIQVAIDALYSQRRRKSPGDESETLTRRNDAIFG
                     NHVWQTFAQYFPPGLEKPSV"
     gene            complement(471..2168)
                     /gene="treA"
                     /locus_tag="RPM73_003323"
     CDS             complement(471..2168)
                     /gene="treA"
                     /locus_tag="RPM73_003323"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDX6521657.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLLHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(2488..3906)
                     /gene="dhaM"
                     /locus_tag="RPM73_003324"
     CDS             complement(2488..3906)
                     /gene="dhaM"
                     /locus_tag="RPM73_003324"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDX6521658.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(3917..4549)
                     /gene="dhaL"
                     /locus_tag="RPM73_003325"
     CDS             complement(3917..4549)
                     /gene="dhaL"
                     /locus_tag="RPM73_003325"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDX6521659.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4560..5630)
                     /gene="dhaK"
                     /locus_tag="RPM73_003326"
     CDS             complement(4560..5630)
                     /gene="dhaK"
                     /locus_tag="RPM73_003326"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDX6521660.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5858..7777
                     /gene="dhaR"
                     /locus_tag="RPM73_003327"
     CDS             5858..7777
                     /gene="dhaR"
                     /locus_tag="RPM73_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDX6521661.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7877..10744)
                     /locus_tag="RPM73_003328"
     CDS             complement(7877..10744)
                     /locus_tag="RPM73_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX6521662.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2612 a         2701 c         2752 g         2878 t
ORIGIN      
        1 atggggcgga tagtgaggcg gttttggctg cgcttattga acatttagaa ttgatcaatc
       61 cttctggcag attaagccaa aatacccgtt cggcaatgct tcagttacgc gaagaggaat
      121 ggtctgaatt ctttttttgg ttgctaaatt ctctggagtg tctggattat gtgattatta
      181 atttaactcc agaaagcaaa aaaacgttga tgagtgaaca ccgtaacaat attcaggtag
      241 caattgatgc tttatatagc caacgtcggc gtaaatcgcc gggtgatgaa agtgaaacac
      301 ttacccgtag aaatgacgct atcttcggca accacgtatg gcaaacattt gcgcaatact
      361 tcccgccagg acttgaaaaa ccgtctgtgt agaaaaaacc atagctatgg tgttatctgc
      421 cgacaacgcc atccggagaa gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg
      481 ggttgtgcct ctttggttga gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc
      541 ggaacattgt cacacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg
      601 ccattggtcc agccaaagcc atcctgtaat ggatattcgc caccgccgcc ccccgttccg
      661 gtggtgctga catcatattt ttccaccagc tttttctccc ggtcataggt gtgctgaaca
      721 ttggtcagga agtgccagct aatgtccatc gccacctctt tttgcccgta gttttgtaat
      781 ccttctgtcg cgacccactg taacggtgcc cagccatttg gcgcatccca ttgttgccca
      841 cttttcaccg acgtggtgtt caggccgccg ggttgcagca gatgtgtttt cgtcgccgtc
      901 gccattttgt tggcgcgatc tttcgctgcc gcattgacgt acagcgggaa cagggcggcc
      961 gcggttaact gattgcgcac tttatgactt ttcaggtcgt aatcggcata ccagccttgt
     1021 tgatcgttcc acatgtattt ttcgatccct ttttgacggg catttgccag cgtttcgtac
     1081 tggtttgcca tcgcgttatc tccggcagct ttgctggcgc gggcgaggat tttttccatt
     1141 ttaaacatca ggctgttcag atcgaccggt acgatgctgg tggtgcgtaa ggtatttagt
     1201 tgatgcgggt tgtccatcca gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc
     1261 aggtcgcggt aaatttcagt ggcaggtcga ttcggattgc ttttggcggt ggcaatatct
     1321 tccacccatg actctggtcg tggcgtatcg cgatcgtccc agtagcggtt gagaagggta
     1381 ccatcctgaa gttttacaac gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg
     1441 tccatccagt aagcatattc tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg
     1501 ccttcatgct gcgccagtaa ctctaccatc agggcaaaga agggcggttg cgagcggctt
     1561 aaatagtaac tgcggttgcc gttgggaata tgaccgtaag tgtctatttc atgagcaaaa
     1621 ttggccacca tatccgcgac tttatcccag tgaccgcttt cggcaagtcc taacatggtg
     1681 aagtaactgt cccagtaata tacctcgcga aaacgtccgc ccggcacgac ataaggttcc
     1741 ggcagcggta acagagaatc ccatttttcg gtgttttcgg tagaacgcgt taataccggc
     1801 caaagtccgt caatatgttc gcgcagtgac tgcccctctg gcggaacata tttctcgccc
     1861 tctttcggca gggtgaaatt gacgttaacg aaatggagca gatcaaatcc gctctggttt
     1921 tgctgcatcc gataatcagc aaggatcatc agcggatcgc tgttcggcac ggcatcggca
     1981 aaggtttttt ggtccggaaa aagtttggcg ttttgcacat cattaaacag cggccctaat
     2041 aaaatatcag gcggctgtgg tgttaccggt gtttcttctg cctgcaccga tagcgcagcg
     2101 aaacacaaaa agatacaggc tggaattaac gccatttttt gcgggcgaga aggtgcgggg
     2161 gatttcatca atcattctcc tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa
     2221 accttagttc aggatcgcac tagctgcatg atcgaaatct cattctgcga actgtcaggc
     2281 attttagaaa agggaattaa agggataaat aacggtgaaa tagagtgatc aaataacgtc
     2341 tgtttattag ccagtcagcg acgttgggtt cgtgattagc gaagaaaaat catcataaat
     2401 tggtgaaaaa tataacaggt agttaaaaac cattagtgct gagtaaattg ccggatgaca
     2461 tcagaacgat gccatccgaa cagtggctta accctgacgg ttgaaacgtt gccttttaac
     2521 gtccagcgtt agcgtttctt ctggttgtat cgcatacagt ttctcaccct gctggcaaat
     2581 ccagccaatc cccagttcac gggcgattag ggcgctgtgg gataccggac ttccggcgct
     2641 aaggcagata ccttttacaa ccgccggatc cagttgcagt actgtggaag gataaatgtt
     2701 ctccgccagt agaatagttg gcgagttaaa ctgcgggagt tcttctttcg tttgggtcag
     2761 gtggaccagg gtgcgatgca gaagatcgtc cacatcaata tagcgagctt gtagatattc
     2821 atcatccagt tgctggtatt gctggctaag ttctttaaga acttgctgcc aggcatattc
     2881 tgccgtgcaa tgttcatgct gaaggagttc gcttgccgcc gccagcagtt ccggatcatc
     2941 taacagtgta tggtgaccag aaaagattgc ggcaatatcg tcaagcccgc tggcttctgc
     3001 ttttgctgtt aacgtcatca gatctaataa cgtgaagtca atagcctggc gtaatcgatc
     3061 ttgttcttct tccacggtca gggttgattt tgcctgtacc gtacataaaa ctggttgata
     3121 ataaaaggct ttacccgaaa caggcggaac gggacgcaga gtaggtggag cgacttcctc
     3181 cgtttcacca aagttatctt cagccagctg acggaaagcg atcagtgcct cttcagcttc
     3241 tggccctttc gcaatcaggc gcagcgtatc gttatagcga acttgtagta acgcaatctg
     3301 gttaatactc tctggtgtga cgcatttgcc gtttttttcc agcaacatat cggcattaaa
     3361 tgtcgataag gtataaacca gccgggaggc cggacgtaca tgcaggccgt tacggttttt
     3421 tatgaccacc gccagagaac gggcttcttc atcgtacgca ggacatgtgt cagagatttt
     3481 agtgtcggag gacggtaaac ccagttgttc acgtttggct tccagcgcat gcatagcgtc
     3541 aaagataact ttgtcgatat ccgctcccga ggccgcgctg accgttgctg ccagtgtacc
     3601 ttcgaccaac ggcgcagcac acaaacgtac ttttgcggcg atcttgggag ccagcaattc
     3661 cagcgcagtt tcagcactca ataatgcgct acccatatcc atcatgacca gcacatggtc
     3721 ggcatcagca acagattcga tggcctccat cactttgacg gcatcggtac caataggatt
     3781 ttgtggatcg tcaattcccg cggcaatggc gattttacaa ctatcactca ttaacatctg
     3841 acgggctaat tcaccgacac cttctcccag tcggctgcta tgtgaaacta tgaccaggtt
     3901 taccatcacc aattccttac tcttttgcgg ctaacgccaa catttgcatc ataaacatca
     3961 ccgaggtcgc gccgggatcc tggtgaccaa tactgcgttc accgagataa ctggcgcggc
     4021 ctttgcgggc ttgcatcgta atcgtacttt gtgcagcgga ttcggcgatt ctactggcag
     4081 cttcgagcgc caccggaaca gagagatttt gctcgctgga ctgacgtaac gattccacca
     4141 ccggcaccca cacatcacac atggttttat cgccaggttc ggctttcccg cgactgatta
     4201 cgccgtccac gccatcgcgg aacatctgat aaagctcttc cagtgtcagg ctttgccgtg
     4261 cctgggtcgc ctgtgcggcg cggataaaga aggtaccgaa cagcggacca ctggcaccac
     4321 cgacgctgga aagcagcgtc ataccggtat tcttgagaat gaaaccgata tctttatctg
     4381 cgatagcagg gagtttttcc accactttgc taaagcctcg gttcatattt agcccgtggt
     4441 cagcgtcgcc aatttcgcga tccagtccgg taagatactc gctctcggtg ctgaaaatat
     4501 cgccacaacg agtgagccag ttaacaattt gagttctgct cagtgacatt gctttctcct
     4561 tatttacccc agttaagggc cggggtgtgg accggggcgt cccagagtgc cagcgtttcg
     4621 tcatcaactt tcagtaaggt gattgagaaa ccggtcatat ccagtgaggt gcagtacgcg
     4681 ccaattaaat tacgttcgat agtcaatccc gcttgctggc aacgtgtggt caggcggtta
     4741 tagacgccgt acagctcaga aagcggagtt gcgccaagat tgttaaccag cgcaatcacc
     4801 cgatcgccag actggagcgg ttgtttggtt tgttgttctt cctgccaact gccttgttga
     4861 taatcccaga aacgcaaagt gcgatggtat gagccatttt ccagcagggt gtcgaacatt
     4921 tcatcgacgg tttgatcaag ggaagagaag gagcggcggt caatacccgg ctcaccatga
     4981 atgccgacgc caaactccat ctcattatcc gccagggtaa aagaaggttt gcccgcggca
     5041 ggaacggtac aggcaccgag agcgatacct attgagtggc cttgattatt caacttacgc
     5101 cccagttccg cacaggcgtc cagtgagtcg ccacgctccg ccgctgcgcc tacgagtttt
     5161 tcaattaata cggtgttggc aacgccgcgt cgcccggcgg tataaagact gtcttttacc
     5221 gcaacgtcgt catcaatgac cacagtggtc acttttacgc cgctatcgtg cagtaactcg
     5281 gtcgctgttt caaagttaag aatatcgccg gtgtaatttt tgataatcaa gagtacacct
     5341 tcgccgccat caatttgcat ggagcattca aagattttat cgggcgtcgg tgaggtgaaa
     5401 atttcgcccg gacaggcccc cgaaagcatc ccctgaccga tataaccaca gtgcatcggc
     5461 tcgtgtccgc tgccgccacc cgacagcagg gcgacttttc ctgcaacagg ggcatcagct
     5521 cgggtgacat acaccggatc ctgatgcagt gtcagcgatg gatgcgcttt cgccagtcct
     5581 gccagttgtt cgtccagtac gtcttgcaca tcattgatca attttttcat tattttgctc
     5641 cagcaattac ggtagggcat ggatgatgtt caacgacacg gcgaccactg actgccgatg
     5701 aatccattgt gcatcaggca agggaaaaga aaattagccc aaattatgtt tcatagtgaa
     5761 acatatgctt taatgaatgt tccatattga aacttttacg tgtattaata cttaaaattg
     5821 cgagccggaa caccttttgt cataagggat gcgggatatg agtggcgctt ttaacaacga
     5881 tggtcggggc atatctccct taattgcaac ctcctgggag cgatgcaata agctgatgaa
     5941 acgggagaca tggaacgtac cacatcaggc ccagggcgtg acatttgctt ctatttatcg
     6001 gcgtaagaaa gcgatgctga cgctcgggca ggctgcgctg gaagatgcct gggaatatat
     6061 ggcaccgcga gagtgtgcgc tgtttatcct cgatgaaacc gcctgcattc tcagccgtaa
     6121 tggcgatccg caaaccttgc agcagctaag tgcactggga ttcaatgacg gcacgtattg
     6181 cgccgaggga attattggta cttgtgcgct atcgttagcg gctatctctg gtcaggccgt
     6241 gaaaacgatg gccgatcaac atttcaaaca ggcactctgg aactgggcct tttgtgcaac
     6301 gccgttgttt gacagcaagg gccgattgac gggaacaata gcgctggcgt gtccggttga
     6361 gcaaactacc gcagctgatt tgccgttgac gttggcaatc gcccgcgagg tcggaaattt
     6421 actgctgacg gacagtttgc tcgctgaaac taaccgtcat ttaaatcaac ttaatgccct
     6481 gttagaaagt atggatgatg gcgtgattag ctgggacgag cagggtaatt tgcaatttat
     6541 taatgcccag gcggcgcggg tcttgcgcct tgacgcgacg gcaagtcagg gaagggcaat
     6601 cactgaactc ttaacgttac ccgccgtatt gcaacaagca ataaaacagg cacatccgct
     6661 caaacacgta gaagcaacct ttgaaagtca gcatcagttt attgatgcgg tgataaccct
     6721 taaaccgata atagaaacgc agggaaccag ctttattttg ttgctccatc ctgtggaaca
     6781 gatgcggcag ttgatgacca gtcaattagg aaaagtcagc cataccttcg ctcatatgcc
     6841 acaggacgat ccgcaaaccc gccgcttgat tcattttggt cgccaggcgg cgcgcagtag
     6901 ctttcctgtc ctgctttgtg gagaagaggg cgtgggaaag gcactgctaa gtcaggcaat
     6961 tcataatgaa agcgagcgtg ctgcaggtcc ttatatcgcc gtcaattgtg agttatatgg
     7021 tgatgcggcg ctggcggaag aatttattgg tggcgatcgc acggacaatg aaaatggccg
     7081 tctgagtcgg ctggaactgg cacacggcgg cacgctgttt cttgaaaaga ttgaatatct
     7141 ggcggtggag ttacagtctg ctttgcttca ggttatcaag cagggtgtta tcacgcgact
     7201 ggatgcgcgg cgtttaatac cagttgatgt caaagtgatt gcaacaacga ccgcggacct
     7261 cgcaatgctg gtggaacaaa atcgttttag tcgccagctg tattacgcgc tgcatgcatt
     7321 tgaaattacc atcccgcctc tgcgtatgcg gcgtggcagc attccggcgc tggtgaataa
     7381 caaattacgc agtcttgaaa aacgcttctc tacgcggctg aaaattgatg acgatgccct
     7441 cgctcgcctg gtttcttgtg catggccagg caacgatttt gaactttaca gcgtcatcga
     7501 gaatcttgct ctgagtagtg ataacgggcg cattcgcgtc agtgatttac ctgaacatct
     7561 gtttaccgag caggcgacag atgatgtcag cgccacccgc ctttccacca gtctgtcatt
     7621 tgcggaagtt gaaaaagagg caattattaa cgcagcccag gtcacaggcg gtcgcattca
     7681 ggaaatgtcg gctttacttg ggatcggccg cactacgctg tggcggaaaa tgaagcaaca
     7741 tggcattgat gcagggcagt ttaagcgccg ggtatgaaag acagaaacga tttctgatac
     7801 atcagagtga tctgtatttc attccggcgc acgctaacaa ttttcagcat cgtttaaggg
     7861 cttgtctatc ccgcacttaa aagctgaagc gatatcctcc gttgacttgc ttctgatcaa
     7921 agaggttacc ctgcgtgtaa tccgcttcga gatagaatgt gtgctgtttg ttatactgtg
     7981 cactgactcc cacgccgtta ttccagccat tacctttgaa actgtacttc tcccgggagt
     8041 tgttcaacag atattcggta tcccctgaaa actcgcggat agcgccagtc ttcacataga
     8101 cattcagctg gctgttgcct gcggtgatgt cataccccag tatcatgctg gcacgcccca
     8161 gcagcgattc gtagtgattc agatgtatat tgaggccatt actcgccttc atagccatct
     8221 cattctggtg gctgtatgta agctgggttt gcggctctat atagaaccca taaccagtag
     8281 gggacaggtt gaacctctgc ccggcttcca gggagatgct cattccattc gcagtgccgt
     8341 tggcgttaac gccgttgttc tgactgtcca gtacgtggaa actatttttc tggcgcgatg
     8401 cttttataac gagatcgctg taaaaaccgt tttgtgccat gtaactggcg tacattccca
     8461 tgtagtctga acgtgcggta ccgtcgcctc cgctatagtc cggcgatgca tgtgttgagc
     8521 caatatacag accgacatac aacggcatta catcagagag acgtttatcc ccaccaaact
     8581 ggataccgct gtaacccatg tcaaagccgc tcagtttgcc actggcaaag gagtccaggc
     8641 ttcccccata actgcgcaac cagatattac cgtctttact ctgatttcgc aggtcaccca
     8701 tccgttgcat cagcgtacgg ttttcaacat agttcaataa gtaaccgaca ttgagataat
     8761 tgccgccagc atctgcggtc gtggtgggtt taggcgtggg agcgggttca ggcgtaggat
     8821 cggggttgac tatgggaggc tgagcgggag ctggtgtggg ttcaggatta ggagtcggtt
     8881 ccggaactgt cccggaagcg taaagctccc agttagtgcc atttttacgc acatcgtaca
     8941 gatatccccc caactcaacc tgagaagacg cgctgaacga ggccgcgccg tcagtggttt
     9001 ttaccactgt cagaacttca tttcccgttg tggcctcgct gccctggttg cggatagcca
     9061 atacgtgatt accagcacta ctcccgctga tattcaataa atccccttta ttattaacgc
     9121 cattaccctc gccaacaaca tcagcacgca taataaaggt actgttaccg ctcaggttct
     9181 ctacgtttaa tgtggcaaat gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg
     9241 aatggctcag cgccagcgtg tcgagattag aattacttgt tacgttccag acgctgttat
     9301 tcattgcaac gtccagtttt ccgccattga cattatcgct gagggaggaa cctgtccaaa
     9361 ccgacccagg gtgcatatcc agattgatta gcccaccttt ggaaagaacg ctaccgttga
     9421 taagcatacg acccgaggcg ttgatgcggc tggcggcata accatcgtca tgttgcgttg
     9481 caatggccat ctggtcgggt gtactcatat caatgaccag atcgctcgtg aggtcgatct
     9541 ggctgttggt catggcataa attcccctgg ctcctgccgc gccggtgata gccaaactgt
     9601 ctccggttat tctaccgccg ctgagcgccc acaaacccag cgccagactg ccattacgat
     9661 caactgtaat atcggtattt tgcatgttga caacagccgt tgccgtctgg gccgaggcac
     9721 cataagaacc gccggaaaag atgctgtttc gttgcgccgc cgtgccagaa aaattgattg
     9781 tcgcgtctga accactggtg acgaggccac cgccctgcgc ggaagaaata tggctatctg
     9841 caccgatagt ggttgtacca ccacgaactt cgacgccatt ggccgcggct ccagttacat
     9901 caacagtgag tgcattcgcg ctaacctggc caaagctcca gaggccgtgt gcattatcgc
     9961 cattggtttt aatggaactg tttgttccga gatcgacaac agagtttttc tgtacgttta
    10021 tccccatggc gctgtagccc tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag
    10081 caccattggc gttattgccg ttgagaccac cgatataaac acctgtactt ccatcggtcg
    10141 tgattttact tccgcttcca agatcgacac tggtgccata gtcattgatg gttaggccta
    10201 taccgttcga gttttcaatg gtgaattgag tggctgttag tgttgagcta tgcccaataa
    10261 tgatgccgtc atcgttactc ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt
    10321 caccaattaa gttaatgccg atggcagagg tttgaccaac aacatctact gtgagtcggt
    10381 tagctgtcag ggagctgttt ttgccctgaa taatgattcc ttttgcatat ccagaggcgt
    10441 cgttgacatt gacggtgaca tcattaccca ggttgatatt gccaggcgtg tttcctgctg
    10501 gggtcattac accatagagg tttcctgatg gatcggcggt atctcctgta attctgtcac
    10561 catcattaat agtaatattg gtgccatatt gacttcctgt atcagcagct atcagcaggg
    10621 gggaaaagat aattgcattc aaaccaatag cgccaaattt tatcagttga attgagggcg
    10681 aacggatttt taattcagcg agacgatctg ctttggtatt cccattgtgt tgtttgatgc
    10741 ccataccact cctatatagt acccagacta tgggtctatg gattttgtca ttttgtttaa
    10801 atcaatgaat aatatcctct tatcatcatg atatttgtat ggtgcaatca tgatgagctt
    10861 ctcagagaat aattctctat taccgggcaa aatcaggaac acaggagtta atatatattt
    10921 aacatccccc ttacaaaggt gag
//
LOCUS       DAPPNJ010000151        10865 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000151 DAPPNJ010000000
VERSION     DAPPNJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.152"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(108..1772)
                     /gene="glnS"
                     /locus_tag="RPM73_003329"
     CDS             complement(108..1772)
                     /gene="glnS"
                     /locus_tag="RPM73_003329"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDX6521663.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1975..3921)
                     /gene="nagE"
                     /locus_tag="RPM73_003330"
     CDS             complement(1975..3921)
                     /gene="nagE"
                     /locus_tag="RPM73_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDX6521664.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4254..5054
                     /gene="nagB"
                     /locus_tag="RPM73_003331"
     CDS             4254..5054
                     /gene="nagB"
                     /locus_tag="RPM73_003331"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDX6521665.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5114..6262
                     /gene="nagA"
                     /locus_tag="RPM73_003332"
     CDS             5114..6262
                     /gene="nagA"
                     /locus_tag="RPM73_003332"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDX6521666.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6271..7491
                     /gene="nagC"
                     /locus_tag="RPM73_003333"
                     /gene_synonym="nagR"
     CDS             6271..7491
                     /gene="nagC"
                     /locus_tag="RPM73_003333"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDX6521667.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7539..8291
                     /gene="nagD"
                     /locus_tag="RPM73_003334"
     CDS             7539..8291
                     /gene="nagD"
                     /locus_tag="RPM73_003334"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDX6521668.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8548..10212
                     /gene="asnB"
                     /locus_tag="RPM73_003335"
     CDS             8548..10212
                     /gene="asnB"
                     /locus_tag="RPM73_003335"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDX6521669.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNSKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10593..10669
                     /locus_tag="RPM73_003336"
     tRNA            10593..10669
                     /locus_tag="RPM73_003336"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10627..10629,aa:Met,seq:cat)
     gene            10679..10763
                     /locus_tag="RPM73_003337"
     tRNA            10679..10763
                     /locus_tag="RPM73_003337"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10713..10715,aa:Leu,seq:tag)
     gene            10787..10861
                     /locus_tag="RPM73_003338"
     tRNA            10787..10861
                     /locus_tag="RPM73_003338"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10819..10821,aa:Gln,seq:ttg)
BASE COUNT         2686 a         2812 c         2780 g         2587 t
ORIGIN      
        1 cgcgaagaaa agcagaaaaa acgccgcaga gtgcggcgtt tgggattgtt tgctatttct
       61 tgccggatgc gacgtaaatg cctcacccgg cctggtagaa cttaaaatta ctcgcctact
      121 ttcgcccagg tatcacgcag cccaacggtg cggttaaata ccggtttttc cgctgtggaa
      181 tggcggctgt cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt acccgcaacc
      241 gcatctttca gcgacggttc agcaaagccc tgtttgatca ccagcgattc cgggttaatc
      301 accgacagga aatcatccgc agcaccaggg ttcggcacgc tgaacagacg gtcgtacaga
      361 cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt gaataacacc tttgacttta
      421 cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg
      481 atattgcctt cggcatcttt ctccacgcgt tcagccttaa tgacgtaagc attacgcaga
      541 cgcacttctt tacccagcac cagacgtttg tactgcttgt tagcttcttc gcggaaatcg
      601 gcgcgatcga tccaaatctc accgctaaac ggcacctgac gactccccat ttccggtttg
      661 ttcggatggt tcggcatggt gaccatttcg ccttcgccct gatagttttc gataaccagt
      721 ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt cgttgagatc ttcacggatg
      781 caggattcca gcgatgccat ctcaatggtg ttgtcctgct tggtcacgcc gatgcgtttg
      841 cagaactcac gaatagaagc agcagtgtaa ccacgacgac gcagaccgga aatggtcggc
      901 atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc
      961 ttggacatca cagtgtattc cagattcagg cgcgagaatt cgtactggcg cgggtgaaca
     1021 ggaatggtga tgttgtccag cacccagtcg tacaggcgac ggttgtcctg gaactcaagc
     1081 gtacacagag agtgcgtaat accttccagg gcatcgctga tgcagtgggt gaagtcgtac
     1141 atcgggtaga tgcaccactt gttgccagtc tggtggtgtt cagcaaattt aatacggtac
     1201 agcaccggat cgcgcatcac gataaacggt gaagccatgt cgattttcgc acgcaggcag
     1261 gctttacctt cttcaaaacc accggcacgc attttttcga acagcgccag gttctcttca
     1321 acgctgcggt cgcggtacgg gctgttttta cccggttgcg tcagggtgcc gcggtattcg
     1381 cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc ctttattgat cagttcgatc
     1441 gcataggcgt ggagctgatc aaaataatcg gaggagtaac ggacgttacc agaccagtga
     1501 aaacctaacc actctacgtc gtttttgatc gactcaacat actcgatatc ttcttttacc
     1561 gggttagtgt cgtcgaaacg caggttgcac tggcctttat agtcctgggc gatcccgaag
     1621 ttcaggcaga tagatttcgc atggccaata tgcagatagc cattcggctc cggcgggaaa
     1681 cgggtgtgta ctgtggtgtg cttaccactg gccagatctt catcgatgat ctgacggata
     1741 aagttagtcg ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg taaacaacgt
     1801 ataacggcgt atgatcttat aagcgggaca ggctgacaac tgttagtttt ttaaaattga
     1861 taatctaaca aggattatgg ggatgattga tgaaaaaaaa agcggcagag aagatctcca
     1921 ccgcttgtca ttgttggatg cgacgctcaa gcgtcgcatc aggcataaag cagattactt
     1981 tttgatttca tacagcggtg tttgacccgc cacaacatgg ccctgagctt taatgatcaa
     2041 gccactgaaa tcgtcgatat tgctgcaaac caccgggcta atcatcgagc gggcgttagc
     2101 gttcaggtaa tccagatcca tttccagaat cggttgccct gcgcttacct gcgcaccctc
     2161 ttccaccaga cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac ccatatggac
     2221 gacgatctcc gcgccttttt cggtttccag gcagaacgcg tggttggtgt tgaagatttt
     2281 cacgattgtc cctgcggctg gtgatacgac gattttatct gtcggtttca ccgccacacc
     2341 gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc tgatccagtg ccacgacatc
     2401 accggtaatc ggcgatacca gctccgcgat agataccgcg tttggtacag cctgcggttt
     2461 tgctacaggc gcggcagttg ccggagttgc ttcagctgac gcagcggcta ccggaccacg
     2521 ggcaacgact ttcttcatcg catcgccgat ggattctgct ttcgcgccaa caatcacctg
     2581 aatagtctgt ttgttcagtt tcactacacc agaagcaccc agacgtttac acatcgtatc
     2641 gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg gtgatacagg cgtcaattgc
     2701 tttcaggttg tcagtgccgc caaccgcagc aatatagttg gtcgccagtt gattgaggcc
     2761 ttcttcagtg ttgctgttgg cttcttcagt aacgatctcg tcttctttat cttcacgacc
     2821 cggcgttttc aggttgaaca tgcggataac caaactgaac accacgaagt agatagcgaa
     2881 gaagacaaca cccataacca gcagcatcca gacgttctgg ctggcggccg gcaggttata
     2941 catcaacgcg tagtcgatag cccccgcaga gaaagagaag ccagcatgga tacccagcag
     3001 tgttgccaca aacaggctga taccggtcag cagtgcgtgc aggaggtaca gcagcggtgc
     3061 aaggaacatg aacaggaatt ccagcggctc agtcacaccg gtcaggaacg cagtaacagc
     3121 aacagaaagc agcatcccgc caaccatcgg acgacgctct ttcggtgctg cgaagtacat
     3181 cgccagcgcc gcacccggca gaccgaacat cataatcggg aagaagccgg acatgaacat
     3241 ccccgcggtg ccgtcaccgg catagaagcg gttgatatca ccgtggaaaa ccgtacccgc
     3301 cgcgttggtg aattcaccaa tctggaacca ggcgatggtg ttcagcacct gatgcagacc
     3361 ggttgggatc agcagacggt tgatgaaacc aaagataccg gaacccagcg cgcccgcaga
     3421 aacgatccac tcgcctcctg catggatagc gtgctgtacc ggcggccaga cgtaaccaaa
     3481 aatggccgcc agcaccaggc agaagaagcc ggtggcaatc ggcacaaagc gtttgccgcc
     3541 gaagaaactc aggaagtccg gcagtttaat atcggaccaa cggttatagg ctgcgccacc
     3601 aaccagaccg gtaatgatac ccgccagtac acccatgtta atttctgggt tgatggtcac
     3661 catcgctttg gttaacacaa agtaacctac cgcacccgcc agcgccgccg cacctgcgct
     3721 gtctttcgac cagctggatg ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat
     3781 cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa tctggctgac cgaatcgcag
     3841 caacagtgcc gccaccggca gcaccgcgat agggagctgt aacgccctac cgagtcgctg
     3901 gaaaaaacct aaaatattca tcttattccc cctacgagaa ccctatttgg ctcgtttaaa
     3961 gccgtatttt tattttgctg caaattgtac tgccgatgtt ctgtaatcag attgttagat
     4021 catctgctac agagtgtgtg aaaatttaat tcgtatcgca aattaaacgc gtgtcttttg
     4081 tgagttttgt caccaaatat cgttatcatc actccctttt actggctaaa gcagaaaact
     4141 tattttatca ttcaaaaaat caggtcggat tgacgcctgt ctgcgcaaat ccaggttacg
     4201 cttaaagatg cctaatccgc caacggctta cattttactt attgaggtga ataatgagac
     4261 tgatccccct gactaccgct gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc
     4321 gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt tctgggcctg ccgactggcg
     4381 gcacgccgat gaccacctat aaagcgttag tcgaaatgca taaagcaggc caggtcagct
     4441 ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa gagcatccgg
     4501 aaagctacta cagctttatg caccgtaatt tcttcgatca cgttgatatt ccagcagaaa
     4561 acatcaacct tctcaacggc aacgccccgg atatcgacgc cgaatgccgc cagtatgaag
     4621 aaaaaatccg ttcttacgga aaaattcatc tgtttatggg cggtgtaggt aacgacggtc
     4681 atattgcatt taacgaaccg gcgtcttctc tggcttctcg tactcgtatc aaaaccttga
     4741 ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa cgatgtcaat caggtgccaa
     4801 aatatgccct gactgtcggt gttggtacat tgctggatgc cgaagaagtg atgattctgg
     4861 tgctgggtag ccagaaagca ctggcactgc aggctgcagt tgaaggttgc gtgaaccaca
     4921 tgtggaccat cagctgtctg caactgcatc caaaagcgat catggtgtgc gatgaacctt
     4981 ccaccatgga gctgaaagtt aagactttaa gatatttcaa tgaattagaa gcagaaaata
     5041 tcaaaggtct gtaattgtta tccctgccct ggctccttgc tcagggcaat attttttaaa
     5101 atcgggggtc agaatgtatg cattaaccca gggccggatc tttaccggcc acgaatttct
     5161 tgatgaccac gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga
     5221 actgccgcca gagatcgaac aacgttcact gaacggggcc attctctccc ccggttttat
     5281 cgatgtgcag ttaaacggct gcggcggcgt acagtttaac gacaccgctg aagcggtcag
     5341 cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa tcaggctgta ctaactatct
     5401 gccgacgctt atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga
     5461 gtacctggca agacatccga atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa
     5521 tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt
     5581 cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg accctggcac cggaaatggt
     5641 tcctgcggaa gtcatcagca aactggcaaa tgccgggatt gtggtttctg ccggtcactc
     5701 caacgcgacg ttgaaagaag caaaagccgg tttccgcgcg gggattacct ttgccaccca
     5761 tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct
     5821 cgacgaagct gacatttatt gcggtattat cgctgatggc ctgcatgttg attacgccaa
     5881 cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt ctggttaccg acgccaccgc
     5941 gccagcaggt gccaacattg aacagttcat ttttgcgggt aaaacaatat actaccgtaa
     6001 cggactttgt gtggatgaga acggtacgtt aagcggttca tccttaacca tgattgaagg
     6061 cgtgcgtaat ctggtcgaac attgcggtat cgcactggat gaagtgctgc gtatggcgac
     6121 gctctatccg gcgcgtgcga ttggcgttga aaaacgtctc ggcacgctcg ccgcaggtaa
     6181 agtagccaac ctgaccgcat tcacacctga ttttaaaatc accaagacca tcgttaacgg
     6241 taacgaggtc gtaactcaat aagagaaagt atgacaccag gcggacaagc tcagataggt
     6301 aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat tgaccagtac
     6361 gggccaatct cgcggattca gattgccgag caaagccagc ttgcccccgc cagcgtaacc
     6421 aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc
     6481 accgggggcc gccgcgctat ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc
     6541 gtacggcttg gtcgtcatga cgccaccatc actctgtttg atctcagcag caaagtgctg
     6601 gcagaagaac attacccgct gccggaacgt acccagcaga cgctggaaca tgccctgctg
     6661 aatgccattg ctcagtttat tgatagctac cagcgcaagc tgcgcgagct gatcgcgatt
     6721 tcggtgatcc tgccagggct tgttgacccg gacagcggca aaattcatta catgccgcat
     6781 attcaggtgg aaaactgggg gctggtagaa gcactggaag agcgttttaa agtgacctgt
     6841 tttgtcggcc acgatatccg tagtctggcg ctggcagagc actacttcgg tgcaagtcag
     6901 gattgcgaag actccatttt agtgcgtgtc catcgcggaa ccggggccgg gattatctct
     6961 aacgggcgca tttttatcgg ccgcaacggt aacgtcggtg aaattggcca tattcaggtc
     7021 gaaccgctgg gtgaacgctg ccactgcggc aactttggct gcctggaaac tatcgctgcc
     7081 aacgctgcta ttgaacaacg ggtgttgaat ctgttaaagc agggctacca gagccgcgtg
     7141 ccgctggacg actgcaccat caaaactatc tgcaaagccg cgaacaaagg cgatagcctg
     7201 gcgtcggaag tgattgagta tgtcggtcgt catctgggca aaaccatcgc cattgcgatc
     7261 aacttattta atccgcaaaa aattgttatt gccggtgaaa tcaccgaagc cgataaagtg
     7321 ctgctccctg ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt tcgtactaat
     7381 ctgccggtgg tacgttctga gctggatcac cgctcggcaa tcggcgcttt tgcgctggta
     7441 aaacgcgcca tgctcaacgg tattttgctc cagcatttgc tggaaaatta atgtgctttt
     7501 atagtggcgc ttattgttgt caatattctg ggtagtccat gaccattaaa aatgtaattt
     7561 gcgatatcga cggcgtgctg atgcacgata acgtcgccgt accgggtgca gcggaatttt
     7621 tgcacgggat tatggataaa ggcctgccgc tggtgttgct gaccaactat ccttcgcaga
     7681 ccgggcagga tctggcgaac cgctttgcca ctgccggtgt cgatgtgccg gatagcgtgt
     7741 tttatacctc tgcgatggcg accgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt
     7801 atgtggtggg cgaaggcgca ctgattcatg aactgtacaa agccggtttc actattaccg
     7861 atgtgaaccc tgattttgtg attgttggcg aaacgcgttc ctacaactgg gacatgatgc
     7921 ataaagcggc ctatttcgtc gctaacggtg cacgctttat cgccaccaac ccggatactc
     7981 acgggcgcgg tttttatccg gcctgtggcg cgttgtgtgc agggattgag aaaatctccg
     8041 ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat catccgcgcg gcattaaaca
     8101 aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga taacttgcgc accgacattc
     8161 tggcgggctt ccaggcgggt ctggagacga ttctggtgct ttctggtgtt tcatcgctcg
     8221 acgatatcga cagtatgccg ttccgcccaa gctggattta cccgtcggtc gctgaaatcg
     8281 acgttatctg aatagcaacc acgtctcagg gcgtggtttc ttatttttac ccatcattaa
     8341 aatcatccca ttcaataaaa ctcgccaaaa attatcgatc catcaataaa tacatcacca
     8401 caccctcccc tttttcacaa atcagcaatc accattacgt tttttatttt tgcactgcca
     8461 aatcgcttgc gcacggtgct gctttgcggc atttttttaa acaagcaaac acaacaagca
     8521 acaaatacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
     8581 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
     8641 ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgccca cgaacgtctg
     8701 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aactcatgtg
     8761 ctggcggtaa acggtgaaat ctacaaccac caggcactgc gcgctgaata tggcgatcgt
     8821 tatcagttcc agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
     8881 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
     8941 gatgcatatc tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac
     9001 gagcacggtc agctgtatgt ggcctcagaa atgaaagccc tggtgccagt ttgccgcacg
     9061 attaaagagt tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat
     9121 tatcatcgcg actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag
     9181 ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgacgt tccttacggt
     9241 gtgctgctct cgggtggtct ggattcctca attatttccg cgatcaccaa gaaatacgcc
     9301 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcaatt acactccttt
     9361 gctgtaggtc tgccgggttc accggatctg aaagcagccc aggaagtggc aaaccatctg
     9421 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
     9481 gtgatttacc acatcgaaac ctatgatgtg acaactattc gcgcttcaac accgatgtat
     9541 ttaatgtcgc gtaagatcaa ggcaatgggc attaaaatgg tgctgtccgg tgaaggttct
     9601 gatgaagtgt ttggcggtta tctttacttc cacaaagcac cgaatgccaa agaactgcat
     9661 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg cgccaacaaa
     9721 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
     9781 gtggcgatgc gcattaaccc gcaggataaa atgtgcggta acagcaaaat ggaaaaacac
     9841 atcctgcgtg aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag
     9901 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag
     9961 caggtttctg atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccaacc
    10021 tcaaaagaag cgtatctgta ccgggagatc tttgaagaac tgttcccgct tccgagcgcc
    10081 gctgagtgcg tgcctggcgg tccttccgtc gcgtgttctt ccgctaaagc gattgagtgg
    10141 gatgaggcgt tcaagaaaat ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg
    10201 gcgtataagt aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt cagttattga
    10261 ggtgcttgtt aattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt
    10321 gacaactcat cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat
    10381 tccgaatttc atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta
    10441 aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaaca gtcactttcg
    10501 agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg
    10561 caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg gttagagcac
    10621 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat ctttttttgc
    10681 gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag
    10741 ttcaagtctc gcctcccgca ccattcacca gaaagcgttg tacggatggg gtatcgccaa
    10801 gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc
    10861 atcga
//
LOCUS       DAPPNJ010000152        10803 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000152 DAPPNJ010000000
VERSION     DAPPNJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.153"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(234..1916)
                     /gene="glcA"
                     /locus_tag="RPM73_003339"
     CDS             complement(234..1916)
                     /gene="glcA"
                     /locus_tag="RPM73_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDX6521670.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2272..4443)
                     /gene="glcB"
                     /locus_tag="RPM73_003340"
     CDS             complement(2272..4443)
                     /gene="glcB"
                     /locus_tag="RPM73_003340"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDX6521671.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRIPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4465..4869)
                     /locus_tag="RPM73_003341"
     CDS             complement(4465..4869)
                     /locus_tag="RPM73_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDX6521672.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4874..6097)
                     /gene="glcF"
                     /locus_tag="RPM73_003342"
     CDS             complement(4874..6097)
                     /gene="glcF"
                     /locus_tag="RPM73_003342"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDX6521673.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6108..7160)
                     /gene="glcE"
                     /locus_tag="RPM73_003343"
     CDS             complement(6108..7160)
                     /gene="glcE"
                     /locus_tag="RPM73_003343"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDX6521674.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7160..8659)
                     /gene="glcD"
                     /locus_tag="RPM73_003344"
     CDS             complement(7160..8659)
                     /gene="glcD"
                     /locus_tag="RPM73_003344"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDX6521675.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8910..9674
                     /gene="glcC"
                     /locus_tag="RPM73_003345"
     CDS             8910..9674
                     /gene="glcC"
                     /locus_tag="RPM73_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDX6521676.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9681..10802)
                     /locus_tag="RPM73_003346"
     CDS             complement(9681..10802)
                     /locus_tag="RPM73_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX6521677.1"
                     /translation="MKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAK
                     NPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAAL
                     FGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLG
                     YHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSG
                     WQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLN
                     GSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARR
                     KIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2537 a         3031 c         2723 g         2512 t
ORIGIN      
        1 gcaaaagcaa cagatagaac ggtgtttgct catcaagcga acataattaa tacagacttg
       61 ttttgcgtga taaaaaaaat agatgttctc acgctcttaa ttatttagca ggttatctga
      121 acacaaaaca ttattgccgc atggataata aagcaagaaa taattttcaa ttaataagac
      181 agaataatgt aaatgaagcc ggatgatatt aacgatcatc cggcattatt gatttacgag
      241 accaacattc cagtaaacac atacgcctgc aacaacgtga taacgccgat aacgctggca
      301 aaaatcagac tgtgcttcac ggtgtagcgg aatagttcag attctcggcc caccatgccc
      361 gtcgcggcgc aggccacggc gatagattgc ggggagatca tcttgccagt tacgccgccg
      421 ctggtatttg ctgccaccag cagagtgtca gagacgttga tttgctgcgc cgtggtcgat
      481 tgcagtgaac caaacagggc gtttgaagaa gtgtccgagc cggtaaggaa tacccccagc
      541 cagccgagga acggtgagaa aaacgggaac atcacgcctg tacctgccag taccagcgcc
      601 agcgtggtgg acatgccgga atagttggtg acgaaggcga acgccagcac catgccaatc
      661 gacaatatcg gccacttcag actaattagc gtttcggcaa agacgccaat ccctttcttg
      721 atgcccacgc cgaggatgaa gatagagata atcgccgcaa taaaaatagc ggtgccgcca
      781 gccgagagag ggtcgaattt aaacaccgca tccattggcg ttggttgggc gacaatgggt
      841 gccgctttca acacttgttg atgcaaatga gggatctgga aattaatcac cagtgaataa
      901 aacgcgccgc ccggagcaaa taacgcttta aacggcttca tggtccagat ggtgaccagc
      961 accgttaaga ttaaaaacgg tgaccacgca cgaatgattt gccccagact atattctgaa
     1021 ggcacgggac cgccagaaga tggcttattt accaccatcg cacctgcgga ttgccccatg
     1081 ctgattgccg tttcggtatt tttcggccgc cagactttaa ggaataaagc gagtgagacg
     1141 atactcacca gcgccgaagt aatatccggc agttccggac caatatagtt agaggtaaag
     1201 aactgagtga cagcaaagct tcccccagca accagcgccg ctggccacgt ctctttcacc
     1261 cctttccagc cgtccatcat tgctaccagc cagaacggca caagaaccga caggaacggt
     1321 aactgacgtc ccgccattgc gccaatgtgg aacggatcga ttcccgttac ctgaccggcg
     1381 accagaatcg gcacgcccaa cgcaccaaac gccactggcg cagtgttggc aatcagacac
     1441 agccccgccg cgtataacgg tttgaagccc aggcccacca gcagcgcacc ggtaatcgcc
     1501 accggcgcac caaagccagc cgctccttcc agtaacgcac caaaggagaa accaatcagt
     1561 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     1621 tgaccgctgg caacggttaa tttatagagg aacaccgccg cgacaataat ccacgcaata
     1681 ggccataatc cataaataaa gccatagccc gcagcagcaa aagccatatc aatcggcatt
     1741 ttaaaggcga atatcgcaat caggatagat aagataaggg ttattgctcc agcgacatgt
     1801 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     1861 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     1921 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     1981 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2041 ctacctctgg cataacaagt atatcgggta agggtttctg ttctgcacac gcaggcgcag
     2101 agtatcgtta agatgtccat attgttgtta taggcccgct agtaatgcgc tacgggtatt
     2161 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2221 ttactatcat agccccgaca ataaaacttg ccggggcttt ttttgacgct attaatgact
     2281 ttctttttcg cgtaaacgcc aggcgtgtaa taacggttcg gtatagccgt ttggctgttt
     2341 cacgccgagg aagattaaat cgctggcagc tttaaaagca cacgagttag cgaaattccc
     2401 cgccatcgga cgataagccg gatcgtcagc gttttgctga tcaaccactt tcgccatatt
     2461 ctccagcgac gcctgcacct gttctttggt cagaataccg tgacgtaacc agttggcgat
     2521 atgctggctg gagatacgca gcgttgcgcg gtcttccatc agcgccacat tgtgaatatc
     2581 cggaactttt gaacaaccaa tcccctgctc cacccagcgc accacgtacc ccagaatccc
     2641 ctgcacgttg ttatccagct cttgttggat ctcttccacc gaccagttag cgttttcagc
     2701 caccggaata gtcagcagat cgtccagcag cggttcaaat tcagcattga actcggtctg
     2761 ggcaatgttg gcttgcacgc tctgtacgtt ggtttggtgg tagtgcagcg catggagcgt
     2821 agcagcggtt ggtgacggaa cccaggctgt gtttgccccg gcacgcagtt ggtcgccctt
     2881 ctggctgtac atgtctgcca tcaggtccgg cattgcccac atgcctttac caatttgcgc
     2941 tttaccgcgc agcccacaga acagaccgga aagcacgtta ttacgctcgt aggctttgat
     3001 ccacggcgtc gatttcatct ggtttttacg cagcatcggg ccagcttcca tcaccgaatg
     3061 catttcatcg ccggtacggt cgaggaaacc ggtattgatg aacgccacgc gattgcgcgc
     3121 ctgagcgata cagctacgca agttcagcga ggtccgacgt tcttcatcca taatgcccat
     3181 tttcagggta ttcggtgcca taccgagcat tgtctcaatg cgggtaaaca gtttgttggc
     3241 gaacgccact tcctgcggac cgtgcatttt cggtttcaca atatagacgc tgccagtgcg
     3301 cgagtttttc tgcactttta aatcatagag ggcgatcgcg ccagtcatga cgccatcaag
     3361 aatgccttcc gggatttcat tgccttcgct gtcccaaatc acaggaatgg tcatcaaatg
     3421 acccacgttg cggataaaca gcagcgagcg tccgtgcaga gaaatttcag agccatcggc
     3481 ggcggtgtaa tgacgatcgt cattcagttt acgcacgatt tgtcgaccgt ttttctccat
     3541 tttctcttgc agagtcccct gcatcaggcc cagcagattg cggtacagca ggattttatc
     3601 ttccgcatca accgccgcga ccgaatcttc gcagtcgaga atggtactga tggccgcttc
     3661 gacgataaca tcgttgatgt gcgccggatc gtctttgcca atccgcccgt tggcatcaat
     3721 ttgcagttca atatgcaggc cgttattttt cagcaaaatg caggtcagcg cagcggcatc
     3781 gccacggtaa ccgacaaact gcgccgggat acgtaacgtg gtttctttac catttttcaa
     3841 ctggatgcgt aattgtttat cgaccacctt aaacgccacc acatcctgat agctgccgtt
     3901 ttccagcggt agagattcat cgaggaaacg ccgaacccag gcgataacct gctcaccgcg
     3961 ttgcggatcg tagccgctga ccatcgcccc ttcctgcggg atgatgtcgc tgccgtataa
     4021 cgcatcgtac agtgagcccc agcgagcgtt cgccgcgttc agcgcgtagc gggcgttcat
     4081 tgccggaacc accagctgtg gccccgcctg actggtgatt tcgctgtcaa tgcccgtggt
     4141 ttccaccgtc acgcgctccg gttgcggcac caggtagccc agttcacgca ggaaagattt
     4201 ataggccgct ttatctttta ccggccccgg attgctgcga tgccactcat caagcgccgc
     4261 ctgaatgcga tcgcgttctg ccagcaactg acgattttct ggtgccagat cgtgaacgat
     4321 ttcatcaaaa ttgcgccaga acgccgcagc gtccagccct gttcccggta aaacttcttc
     4381 atccacaaaa cgtttaaaat tggcgtcaat gcgtaaacgg ccctgggtta tggtttgact
     4441 cattgtttat ctcctcgttt tcgcttattt cgccaacacc gctgctgccg ctttcgcgac
     4501 ctgcgcatcc tgtgctccgg ttaaaccaga aacgcccacg gcaccaataa tttgcccatc
     4561 aacaacaacc ggtacgccac cttccagcga cgtcagtaac ggcgcagtca cgaacgcggt
     4621 acgtccgttg ttcaccatct cttcatagcc cttagtttca cgacgcccca gcgcggcggt
     4681 acgcgctttc tcctgggaga tgtaagccgc aatcggcgcg caatcgtcca tgcgacttaa
     4741 cgccagcaga tgaccgccgt catcggcaac agcaatggaa acagaccagt tatttttctg
     4801 cgcctcttcc tgacctgcgg caataattgc actcgccatt tgctggctaa gaatgacttt
     4861 agttttcatt tttttattcc ttttcaaggg cttgttctac aatttcaatc cagtgacgca
     4921 cagaggtacg accggcgctc gccagatgcg tctggcaacc aatgttggcg gtgacgatca
     4981 tttccggttt gccgctttcc agcgcattca ttttgttatc ccgcagctgg cgtgccagat
     5041 cgggatgcgt taacgcatat gttcccgctg aaccgcagca cagatggctg tcgggaacgt
     5101 ccgttaaggt aaatccaaga cgaagcaaca ctttttccac ttcgccgttc agcttttgcg
     5161 catgttgtag ggtacacggg cagtggaagg ccagtttttt atcgccgcga actgccagtt
     5221 tttccagcgg ttcctcgcgc agaagctcga ctaaatcgac cgccagttca ctgacttgac
     5281 gcgctttatc ggcatataac gcatcgtttt tcagcatctg cccgtactct ttgacaaacg
     5341 cgccgcaacc gctggcggtt tgcaaaattg cctcggcacc tgcttcaatc gcgggccacc
     5401 aggcatcaat attattgcgc gcccgtgcca gccctttctc ctgcgcatta agatgatagt
     5461 ccaccgcgcc acaacagcct gcttcgttag ctgacatgac gctgatcccc agacgatcca
     5521 gcactcgcgc agttgccgcg ttggtgttgg gcgaaagcgt aggctgggcg cagccttcca
     5581 gcattaaaac ccgacgctta tggcgcagcg gcggacgcgg tttagctttc accgtttcag
     5641 caggcagttt tgctctgacc tgttccggta aaaacggtcg cagcaccagc cctacctgcg
     5701 tcagcgcacg gaagaccgcc ggacgcggca ctacctggcg caatccttcg cgcagcattc
     5761 gctccggcag tgggcgtttc actttctgct cgacaatatc acgcccgata tccagcaaat
     5821 tgtgatagcg cacaccagaa ggacaggtgg tttcacaatt gcggcaagtg aggcagcgat
     5881 cgagatgctc ctgtgtttta agcgtgactt cgttgccttc cagcacctgt ttaatcagat
     5941 agatgcgccc gcgcggcccg tccagttcat cgcccagaag ctgataggtt gggcaggttg
     6001 cggtacaaaa tccgcagtga acacaggcgc gcaggatgct gtcggcttcc agcgcgcgcg
     6061 cgttctgccg catctcttca gttaattggg tttgcatagc ctgctcctca aagttccgcg
     6121 tacatgcgac cggggttaaa cacgccgcaa gggtcgagct gctgtttaag ctgctggtga
     6181 tagcggaata aaggagccga tagcggggca aagccaccat ctccggcact aaagcgggtc
     6241 gcatgaccgc cagcgttgcg ggcgatgcga tggatttgat tgtcctcggc tgtcgatttc
     6301 agccagcgta acgctccgcc ccagtcgatc agttgctcgc cgggtaaatc catcatcggc
     6361 gcatcgctgg gtaatgaaat gcgccataag gtacctggta acgagaagaa cggcagttgt
     6421 tgttcacgca attgctgcca gaactgacca gcaacctctt cgccccccag cagttcacgc
     6481 gccgctttta ccgatccttc gccgccctca aggcggatcc acaacgcatt gtcgaagtaa
     6541 cataagccac taataggtag cggctggagt tgccactggg cgatttcgtt catggcttct
     6601 tgcaggctga tttcccgacg caggctcagg gaggcgcgcg gtcgcggtaa cactttcatc
     6661 gagatttcag tgagcacgcc aagacaaccg tagcttccgg ccattaaccg tgagagatcg
     6721 tatccggcaa cgtttttcat cacttcgcca ccaaaacgca gatgttttcc agcgccggta
     6781 atgatgcgcg tgccgaggac aaaatcgcgg accgaaccgc tccacgggcg acgcggcccc
     6841 gccagcccgc aggcgaccat cccaccccag gtggcttctt caccataatg cggcggctcg
     6901 cagggaagca tttgcccggc gctctccagc gccgcttcaa tcgccaccag cggcgttccg
     6961 gcacgcgcgg ttatcaccag ctcggtcggg tcgtaattaa caatgccgcg atgacaacgc
     7021 acatccagcg tttgcccggt gacagggcga cctaaaaagg ctttgctatt gctgccctga
     7081 atcaccagcg gcgttttatc gctaattgcc tgattcacct gctccagcag cgcctggctg
     7141 taatcacact cgcgtagcat cagaaacgct ccagttcagg gaaaggtaaa tgaccgtgat
     7201 gcacatgcat ggcaccaaat tcagcacagc ggtgtagcgt gggaatgttt ttcccagggt
     7261 tcagcaaacc atcggggtca aacgccgcct tgaccgcatg gaaggtcgtg atttcatcgc
     7321 tgttgaactg ggcgcacatt tgattgattt tttctcgccc gatgccatgt tccccactga
     7381 tgctgccgcc aacttcaacg cagagttcga ggatcttccc acccagctct tccgcgcggg
     7441 caaattcacc gggttcgttg gcatcgaaaa ggattaacgg gtgcatgttg ccatctccgg
     7501 catgaaagac gttggcaaca cgtaaatcat attgctgcga taaacgggca atgccttcca
     7561 gtacgccagg cagggcgcga cgcgggatgg tgccatccat gcagtagtaa tccggggaga
     7621 tacgtcctac cgccgggaac gcatttttgc gaccggccca gaaacgtacg cgctctgctt
     7681 cgtcctgtgc cagacggacg tcagtcgcgc ccgctttcaa caagatgtcg ttaacccgct
     7741 cgcagtcttc ctgtacgtca gactccacgc cgtccagctc gcataacaaa attgcttcgg
     7801 cgtcgacggg ataaccggca tgaataaaat cttccgccgc gcggatcgac aggttatcca
     7861 tcatctccag cccgccgggg ataatgccat tggcgatgat gtcaccaact gcaagtccgg
     7921 ctttttctac cgagtcaaag ctggctaaca gaacccgcgc cacgggcggc ttcggcagca
     7981 gttttaccgt cacttcggtg gtcacgccga gcataccttc cgatccggtg aacagcgcca
     8041 gcaggtcaaa accaggtgaa tccagcgcgt ccgatcccag cgtcagcgcc tcgccgtcca
     8101 gcgtttgcac ttcaattttc agcaggttat gtacggtcag accatatttc aggcagtgga
     8161 cgccgccggc attttctgcc acgttgccgc caatggaaca ggcgatttgt gaggaagggt
     8221 ccggtgcgta gtagagatta tgcggtgcaa cggcctggga gatcgccagg ttacgcacgc
     8281 ctggctgcac gcgcgcgcgg cgaccaacgg ggttaatgtc gaggatctct ttaaagcgcg
     8341 ccatcaccaa caacacacct ttttccagcg gcagcgcgcc accagaaagc ccggtgcctg
     8401 caccacgggt caccaccggt acacgcaggc gatggcagac agcaagaatc gctgtcacct
     8461 gttccatttg cttaggcaga acaaccagta atggacgcgt gcgatacgcg ctcaacccgt
     8521 cacactcgta aggaatgatc tcctcatcgg tatgcaggat ctcaagtcca gggacatgct
     8581 cacgcagtgc catcagtacc gatgtgcggt cgacatcggg taaagcgcca tcaagacgct
     8641 cttcgtacaa gatgctcatg agtaggcttc gctttgttgt gttgtgtggc agctgatttt
     8701 tgcgcgctgc ttctgtgaac agttattaag cgggcttttc gttttcgtct atctctttag
     8761 ctaccggtca gaccattttt tttccagctc tgtgaccttg ccttggttaa ctcaatgtta
     8821 aattgatgta acataatcac ttacgtgatg tgcgtgtttt gcgagttaag aacagaaaaa
     8881 ttggtcctac ctgtgcacga ggtccgggaa tgaaagatga acgtcgccct atttgcgaag
     8941 tggttgcaga gagtatcgaa cggttaatta tcgacggcgt actgaaggtc ggtcagccac
     9001 ttccctcgga acgtcgactg tgtgaaaagc tcggcttctc acgctccgca ctgcgtgaag
     9061 ggctgaccgt gctgcgcggg cgcgggatta ttgagacggc gcagggtcgc gattctcgtg
     9121 tcgcacggct taatcgggtg caggacacca gcccgctgat ccatctgttc agtacgcagc
     9181 cgcgaacgct gtacgatctg ctcgacgttc gcgcattact ggagggcgaa tcggcaaggc
     9241 tggcggcaac gctgggaacg caggctgatt ttgttgtgat aacccgctgt tatgaaaaaa
     9301 tgctcgccgc cagtgagaac aacaaagaga tttcgctgat cgaacatgcg cagttggatc
     9361 acgctttcca tctcgccatt tgtcaggctt ctcacaatca ggtgctggtg tttacgctgc
     9421 aatcattgac cgatctgatg tttaattcag tgtttgccag cgtaaataat ctctaccatc
     9481 gaccacagca aaaaaagcag atcgatcgcc agcatgcgag gatctacaac gcggtgttgc
     9541 agcggctgcc gcacgtcgcc cagcgcgcag cacgcgatca tgtgcggacc gtgaaaaaga
     9601 atctccacga tatcgagctg gaaggccacc atttgattcg ctcggcggtg ccgctggaga
     9661 tgaacctgag ttagctggta ttaaatctgc ttttcataca atcggtaacg cttgtacggc
     9721 tccgccccaa tgcgttccag catgttattc atgcctgtat tggtttcgag gatccatgac
     9781 atctccagcg catcgatctt ccggcgggca aacggatcgc gtaaggcttc aatcaataac
     9841 agcgcaatca ccgggccgat gcggctgaac tgatactcgt cgcgcacgcc catcagcggt
     9901 actcgcgcag ttcgcacacc gctgactttc aaacgccaca gcaattttgc ccagccgaag
     9961 ggaaagagcg atccgttcag atcggcaatc gcctcgttga tgttcggcaa gccgacaata
    10021 aacgcgcagg gtgcagaatc aatctcagcg atatagatca tatcgtccgg caccagatat
    10081 ttaagttgat cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg
    10141 tgctgccagc cagagttgaa aatctcacgc aggatctgca tttcttcggc aaaccgctga
    10201 cgattgatgc agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc
    10261 ggagagaaag tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga
    10321 taacccagtt gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag
    10381 ggtggtgtgt caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa
    10441 ggaccgctga tcttacttgc accttgtgac ttcaaccacg cttccgctgc gccaaacaac
    10501 gcggcaaaaa cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca
    10561 ccgtaacgct cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc
    10621 gctttttttg ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac
    10681 aaatgctcgt tgcgttctat gaataaaggg ggtatccagt ttggatcgtc gggataaagt
    10741 gatgacggaa aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta
    10801 atc
//
LOCUS       DAPPNJ010000153        10784 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000153 DAPPNJ010000000
VERSION     DAPPNJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.154"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..742
                     /gene="adiY"
                     /locus_tag="RPM73_003347"
     CDS             <1..742
                     /gene="adiY"
                     /locus_tag="RPM73_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDX6521678.1"
                     /translation="PCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNIIDVSSLN
                     NTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAAQHSMMPA
                     ETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIHKDWNLSM
                     VASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQSCGYNST
                     SYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            879..2216
                     /gene="adiC"
                     /locus_tag="RPM73_003348"
     CDS             879..2216
                     /gene="adiC"
                     /locus_tag="RPM73_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDX6521679.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2320..3963
                     /gene="eptA"
                     /locus_tag="RPM73_003349"
     CDS             2320..3963
                     /gene="eptA"
                     /locus_tag="RPM73_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDX6521680.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            3960..4628
                     /gene="basR"
                     /locus_tag="RPM73_003350"
     CDS             3960..4628
                     /gene="basR"
                     /locus_tag="RPM73_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDX6521681.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4629..5729
                     /gene="pmrB"
                     /locus_tag="RPM73_003351"
     CDS             4629..5729
                     /gene="pmrB"
                     /locus_tag="RPM73_003351"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDX6521682.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5705..5794)
                     /gene="pmrR"
                     /locus_tag="RPM73_003352"
     CDS             complement(5705..5794)
                     /gene="pmrR"
                     /locus_tag="RPM73_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDX6521683.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5906..7408)
                     /gene="proP"
                     /locus_tag="RPM73_003353"
     CDS             complement(5906..7408)
                     /gene="proP"
                     /locus_tag="RPM73_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDX6521684.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7672..8550)
                     /locus_tag="RPM73_003354"
     CDS             complement(7672..8550)
                     /locus_tag="RPM73_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDX6521685.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8547..10775)
                     /gene="crfC"
                     /locus_tag="RPM73_003355"
     CDS             complement(8547..10775)
                     /gene="crfC"
                     /locus_tag="RPM73_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDX6521686.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2703 a         2827 c         2664 g         2590 t
ORIGIN      
        1 accttgtatt gttttattga ctgaaaaaga tgtctggata agggtgaatg ggaaagaacc
       61 tattagcctt aaagctaacc atatggcgtt attaaattgt gaaaataata ttatcgacgt
      121 ctcctctctt aacaacactt tggttgctca tattagtcac gacatcatca aagattacct
      181 ccggtttctg aataaagatc tctcgcaaat accagtatgg caacgtagcg ctacgcccat
      241 actcaccctg ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac acagcatgat
      301 gcccgcagaa actgagtcag aaaaggaacg aacacgcgca ttattattca cggtgctatc
      361 ccgttttctc gacagtaaaa aattcctttc attaatgatg tatatgttac gtaattgtgt
      421 aagtgacagc gtttatcaaa ttattgaaag cgatattcac aaagactgga atcttagtat
      481 ggtagccagt tgtttatgtc ttagcccaag tctgttaaag aaaaagttga aaagcgaaaa
      541 caccagttat agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa atgaattaat
      601 gatggacggt aaaaatatct ctcaggtatc acagtcctgc ggctacaaca gtacgtcgta
      661 ctttatttct gtctttaaag acttctacgg tatgacgccg ctgcattatg ttagtcagca
      721 cagagaacgc actgtcgcct gatttttaac cttaacgaag agctatatta ataacggcat
      781 cagcgataac ccggtcgata ataattcaac tatcgaatgc aggcgtatga tatgacgtaa
      841 tttattgtca cgaagctcgc cttcgcagga gtttaattat gtcttcggat gctgatgctc
      901 acaaagtggg cttaatcccc gtcaccctga tggtgtcggg gaatattatg gggtcaggtg
      961 tttttctgtt acctgcaaac ctggcctcta ctggcgggat tgccatttat ggatggttgg
     1021 tgacgattat cggtgcactg gggctctcga tggtatacgc caaaatgtcg ttcctcgacc
     1081 caagtcctgg tggttcttac gcttacgccc gccgctgctt tggcccgttt ctcggttatc
     1141 aaaccaacgt cctctactgg ctggcctgct ggatcggcaa tatcgccatg gtggtcattg
     1201 gcgtaggata tttaagttac ttcttcccga ttctgaaaga tccattggta ttaaccatca
     1261 cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg aaaatgatca
     1321 cccgtgtgca ggcagttgcc accgtgctgg cgctgatccc catcgtcggg attgccgtat
     1381 ttggctggtt ctggttccgt ggtgaaacct atatggcggc atggaacgtc agcggcctgg
     1441 gcaccttcgg tgcaattcaa agtaccctta acgttacgct gtggtcgttc atcggtgtgg
     1501 aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc cctatcgcca
     1561 ccattggtgg ggtattgatt gccgccgttt gctatgtact ttctaccacc gcgattatgg
     1621 ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc attcggtgat gccgcacgga
     1681 tggcgttggg tgacaccgcc ggggccattg tttccttctg cgcagctgcg ggttgcttag
     1741 gttcactggg cggctggacg ttgctggcgg gtcaaacggc gaaagccgct gccgatgacg
     1801 gactgttccc accaattttt gcccgtgtaa ataaagcggg tacgccagtg gcggggctga
     1861 ttatcgtcgg tattttgatg accatcttcc agctcagcag catttcacca aacgcgacca
     1921 aagagttcgg tctggtttct tccgtctcgg tcatctttac actggtgcca tatctttaca
     1981 cctgtgcggc gttactgctg ctcggacacg gtcactttgg taaagcacgc ccggcatatc
     2041 tggcagttac taccattgcc ttcctctact gcatctgggc cgtggtgggg tccggagcga
     2101 aagaggttat gtggtcattt gtcaccctga tggtcatcac cgccatgtat gcgctgaatt
     2161 acaaccggct acataaaaac ccgtatccct tagatgcacc aataagcaaa gattaattcc
     2221 ccttaatcca gcaaacataa aagccaacct taagaactta aggttggctt aattttgctt
     2281 tgcgagcata tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg cctactaaaa
     2341 agaccctctt tgaatttact cgcctggcta ttgttggccg ctttttatat ctctatctgc
     2401 ctgaatattg ccttttttaa acaggtgttg caggcgctgc cgctggattc gctgcataac
     2461 gtactggttt tcttgtcgat gccggtcgtc gctttcagcg tgattaatat tgtcctgaca
     2521 ctaagctctt tcttatggct taatcgacca ctggcctgcc tgtttattct ggttggcgcg
     2581 gctgcacaat atttcataat gacttacggc atcgtcatcg accgctcgat gattgccaat
     2641 attattgata ccactccggc agaaagttat gcgctgatga caccgcaaat gttattaacg
     2701 ctgggattca gcggcgtgct tgctgcgctg attgcctgct ggataaaaat caaacctgcc
     2761 acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata ttctggtttc tgtactactg
     2821 attttgctgg tcgccgcact gttttataaa gactacgcct cgttgttccg caataacaaa
     2881 gagctggtga aatccttaag cccctctaac agcattgttg ccagctggtc atggtactcc
     2941 catcagcgac tggcaaatct accgctggtg cgaattggtg aagacgccca ccgcaacccg
     3001 ttaatgcaga acgaaaaacg taaaaatttg accatcctga ttgtcggcga aacctcgcgg
     3061 gcggagaact tctccctcaa cggctacccg cgtgaaacta acccgcggct ggcgaaagat
     3121 aacgtggtct atttccctaa taccgcatct tgcggcacgg caacggcagt ttcagtaccg
     3181 tgcatgttct cggatatgcc gcgtgagcat tacaaagaag agctggcaca gcaccaggaa
     3241 ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc tgtggaatga caacgatggc
     3301 ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct gaatctgcct
     3361 ggtcagtgca tcaacggcga atgctatgac gaagtactgt tccacgggct ggaagagtac
     3421 atcaataacc tgcagggtga tggcgtgatt gtcttacaca ccatcggcag ccacggtccg
     3481 acctattaca accgctatcc gccgcagttc aggaaattta ccccaacctg cgacaccaat
     3541 gagatccaga cctgtagcaa agagcaactg gtgaacactt acgacaacac gctggtttac
     3601 gtcgactata ttgttgataa agcgattaat ctgctgaaag aacatcagga taaatttacc
     3661 accagcctgg tttatctttc tgaccacggt gaatcgttag gtgaaaatgg catctatctg
     3721 cacggtctgc cttatgccat cgccccggat agccaaaaac aggtgccgat gctgctgtgg
     3781 ctgtcggagg attatcaaaa acggtatcag gttgaccaga actgcctgca aaaacaggcg
     3841 caaacgcaac actattcaca agacaattta ttctccacgc tattgggatt aactggcgtt
     3901 gagacgaagt attaccaggc tgcggatgat attctgcaaa cttgcaggag agtgagtgaa
     3961 tgaaaattct gattgttgaa gacgatacgc tgttattgca gggattgatt ctggcggcgc
     4021 aaactgaagg ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa caaagccttg
     4081 aggccggtca ttacagcctg gtggtactgg atttagggtt acccgacgaa gatggactgc
     4141 attttctcgc ccgtatccgg cagaaaaaat ataccctgcc ggtactgatc ctcaccgctc
     4201 gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac tatctggtga
     4261 agccttttgc gctggaagag ttacacgccc gtatccgcgc cctgctacga cgccataata
     4321 atcagggcga aagtgagctg attgttggca atctgacgct gaatatgggt cgccgtcagg
     4381 tatggatggg cggtgaagag ttgattctga cgcccaaaga atatgctctg ctgtcacggt
     4441 taatgctcaa agcaggcagt ccggtgcatc gggaaattct ctacaacgac atctataact
     4501 gggacaatga accctcgacc aacaccctgg aagtgcatat ccacaatctg cgcgacaaag
     4561 tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta tatgctggtc gcgaatgagg
     4621 aaaactaatt gaatctgatg cattttctgc gccgaccaat atcgctgcgc caacggctga
     4681 tattgaccat cggggccatt ttgttggtgt ttgagctgat cagcgtcttc tggctatggc
     4741 atgaaagtac cgagcagatt cagctgtttg agcaggcgct acgcgacaat cgcaacaacg
     4801 atcgccacat catgcgtgag atccgcgagg cggtcgccag cctgattgtc cccggcgtct
     4861 ttatggtcag cctgacgcta tttatctgct atcaggcggt acgccgcatc acccgcccgc
     4921 tggcggagct gcaaaaagag ctggaagcgc gcaccgccga caacttaacg cccattgcca
     4981 ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc gttgaacgat ctggtcagtc
     5041 gcctgaccag cacgctggat aacgaaaggt tgtttaccgc tgacgtcgcg cacgaactgc
     5101 gaacgccact ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg catcacattg
     5161 atgtagcacc gttagtggca cggcttgatc agatgatgga gagcgtctcc cagctgctgc
     5221 aactggcgcg tgccggacag tcattttctt ccggtaatta tcaacatgta aaactgctgg
     5281 aagatgtcat tctcccctcg tatgacgaac tcagtaccat gctcgaccag cgacagcaaa
     5341 ccctgctatt gccagagagc gccgccgaca tcaccgttca gggtgatgca accctgctgc
     5401 ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa ggcagcaaca
     5461 ttatgattaa gctgcaagaa gatggcggag cggtcatggc agttgaagat gaaggaccag
     5521 gtattgatga gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg gacagccgtt
     5581 atggcgggat tggtctgggg ttaagtattg tcagccgcat tacacagttg catcacgggc
     5641 agtttttcct gcaaaaccgg caagagactt ccggcacgcg ggcctgggta cggctgaaga
     5701 aagatcagaa cgtggcaaac cagatataaa gaaagctgct gaccaccagc acgctgaaca
     5761 cggtagttaa actttcataa acacggtttt tcattgcact ctcctccgtt aacctggagg
     5821 agagtatgcg cgtcagagat taagtgaacc ttaagagttc aattggcctg ataagacagc
     5881 gtcacatcag gccatccgtt tcagcttatt catcaattcg cggatgttgc tgcaccaggc
     5941 gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat attatcgatt ttctgctcga
     6001 tattatcgta atgctcgacg agaatttcct tcgcttcctg tatatctgac gccgccggtg
     6061 tcgcaccttt caacggacga tttgccgtct ctttcatggt tacgccggtg attaaaccaa
     6121 tcaccgccac taccatcagg taataggcag gcatcatcag attctgcgag ctttcgacca
     6181 gccaggccgc cagtgttggc gtcagaccgg caaccagcac cgaaatatta aatgccgccg
     6241 ccagcgcgct gtaacggata tgcgtcggga acatcgctgg caaggtagaa gccataacgc
     6301 ccgtaaagca gttaaggatc accgccagca tcagtaaccc ggcaaaaatc aggccgatga
     6361 cgttactgtt aatcagaata aacgccggga tcgccaacac aaacagggca acactaccaa
     6421 gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa gcccatcacc ggctggacaa
     6481 acagcatacc gatcataatg gcgataataa tcagcacccc gtggtcttcg gagtaatgca
     6541 ggttatgcga caaataactc ggcatatagg tcagcaacat gtagtaagtc acgttggtgg
     6601 caattaccag accaatacat gtcaacaggc tgcgccagtg tttggtggca atctctttaa
     6661 acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt ttatcgacat
     6721 gctgctggaa cgccggagtt tcttccagcg catggcgcag gtaaagcccg ataatcccta
     6781 acggcagagc aataaagaac ggaatacgcc agccccagtc gaggaagttc gcttcgccga
     6841 caatggtcga aattaacacc actacgccag cacccagcac aaacccggca atagaaccaa
     6901 agtccagcca gctgcccata aagccacgtt tacggtcagg ggagtattcc gcaacaaata
     6961 tcgacgcccc ggtatattca ccgccgaccg agaaaccttg tgccatctta cagatcaaca
     7021 gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca atacagaacg
     7081 tactgatcga cataatcaca atagtgatag cgaggatctt ctggcgacca tatttatcgc
     7141 ccaacatacc aaagaagagt ccgccaagcg gtcgaatcag aaagggaacg gagaacgtgg
     7201 caagtgcagc aaccatctgc acgctggggt cagcccccgg gaaaaaaact ttacctaatg
     7261 cgtaagcaac aaaaccataa acaccaaaat caaaccattc cattgcatta cccagtgatg
     7321 ctgcggtaat ggctttacgc agtttaccgt catcaataat ggtgacatca cgaagggtaa
     7381 tcggttttac ttttttcctt ttcagcatag ctttcctcgc agagttggcc catttccgca
     7441 aacgccagaa aaatcagcgc gacggccgca aaaggggagc gggaattgca ggatgcaatc
     7501 tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt aaggaatgta caatcaaacc
     7561 agttgacgcc tgaattgggc agttaatgac ctttttaccc taccagtgtt taaatttgtg
     7621 atcaacttca cacatggatt tcgtttagga ctcattgatg taactgatta tttacgccaa
     7681 atttacagag aagatttcct cgaccattgc ccccgccaga cgctgcgctg aacagagtcg
     7741 tggcatcccc agcgcgacag tttgccagcg ttgggtcact gaccagcaaa cgggttgttt
     7801 atctgccgtg gtccactttc ccagccaact taaaaaatca gcatgatccc cgcccgtcgg
     7861 aacggtcgaa gaggcaaacc actgatggta atagtgacgc gtggtcgggg ccgcattgat
     7921 actctctgcc agatgttgtg tcgctttcac gcgcaaatca cccagcaaca acgccaccgc
     7981 ttgcgggctt cccagacgac aggcatcgcc aaatgccccc caacgcaact cttcgagaac
     8041 gataaaacag cgcccaggca acgaccaggc gttatacgcc ccagcacgcc agcgggtaaa
     8101 aatttgctcg ctctgttcac ccgcctgcac cagtaggcct cgttgcgtta atgccttttc
     8161 cttccagcga caacgggctg aaaaccactc atacaaggcg gcaatttgct gggggagctc
     8221 cgggaaagca gagtgctggc ggtgatccat tttttgcagc agcgccagat gctgcgcgat
     8281 ctgcgtcagt gccagatgac cgggatccag catcgacgcc agtttttcca ccaatgtcag
     8341 acgcaggcta agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa gctgcacgcg
     8401 cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc tgatgggctt gtggaaggcg
     8461 ggcatcatcg ccctgcgcca gatccactaa aaagcgggga taaaccgact ccagcacgcg
     8521 accggggccg tcaagtaacg tcttggtcaa tatcgttctg ccgtgaaaag tgtttgaata
     8581 tcatcgcgta acagctgggc gtcggtgtaa atccagccgt gagtcatcac agtctgctgc
     8641 aattgctgct gcatcagcct gaccaccgat tcattttgtt gacgcagagc cagactttcg
     8701 cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact cagcgaaaaa agtattcatg
     8761 cctgccgtaa cagattcatc gacctgctct gccagaactt tagccatttg ttggcaataa
     8821 agatcgactt ctgcgcttaa agctcgttgc aacacactgt aatcaaccgt ttctgtcggg
     8881 gatttctcat ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa agtctcacgc
     8941 acaacgccta aacgcgtgct ctgctcgtcc gttgcatcct ggcgcgaaat gacttcactg
     9001 aacagctggc gagtgttgaa gtggggaact acgccgtgaa aaacaggaaa atgaaaccca
     9061 ggacgaaacc ctgactcgct caattccatt ttgacttgtt gctcaatggg ccgaataaca
     9121 tcggttaaca cccggcaaag ggtggattca agctcggcaa aacgcagcgt aaagtcgcga
     9181 ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc aggtacgaat ctcgcttaac
     9241 ggctccgaat catcctgaaa caaggcggct aactgcgcat tcagcgcatc ttgctgttga
     9301 cgcagaaagt ggttggcgga ggtcagggcc agctcgattt catgtttaac ctcgccgctc
     9361 acctgcgcct gattgagttg caatagctgc aaactttctt cgacctgatg gatattttgc
     9421 cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa aatccaggta tccccgcgcc
     9481 tgctgcgcgt aattcaacag tttatgcgca gcagatcgca gagcatacaa cgaggcgtta
     9541 gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga acagcgaatc ttcccacaac
     9601 tgatcggcag catgacgaat atgttcgagg tccgccagat cgtcatgacg ccagcgcctg
     9661 ccgagcgcag catgggcaaa atcttccacc cagcgttgtt gctctggcgc tggtaactta
     9721 ccgttgttgg ctaactcatg gcgcgctcga ttcgccaggt agccccacat cgacgacacc
     9781 ggaaatatct gctgtggcgt aatacagcct ttcatcagcg tcccggaaat cagtgcccgc
     9841 acctggtcgg cgtcgtcact gttacgatcc tgttgatcga acttattgac cagcacatac
     9901 agcggcaccg attgccccac cgccaaaatc gcctcacgga cctcttcatc ggagatcgat
     9961 ttcagttgcg tataatccag caccgccagt accgccgagg cgcgtgccag ctgctggtta
    10021 agcatttttt gcagatgcgg ttgcccggct tcatttggcc cgggggtatc cagtaacgtc
    10081 aactgaccgg gataactctc cagccccgcc agatggacaa actccacttc aatcacggga
    10141 atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc cttcgccagt
    10201 cgcactaaat cattcaaact tttcagacaa tgaaaaatag gctgggcacc cagataatat
    10261 ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg cacgcatatc tttatctatt
    10321 tccagcacat cggtcagatg cttaatatcg caatcacgca ggcgctgttg taattgttga
    10381 attaaacaat cgattggcgc gacatgtgaa aaatgcagta ccggttcctt ttgcccgggc
    10441 gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat tacgattagg cagaacctcc
    10501 gtaccaacaa tggcattaat ggtggttgat ttccctgctt tcatggtacc gacaattgcc
    10561 agcaccattt ccagtcggga aattttacgc aactcattat tcagcatcgc gtgacgttcg
    10621 gcgatattag gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc gggtacagag
    10681 agaggcattt tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag gcgttcagcc
    10741 tcctgactta actcatacag ggtctgtgtg tacatagaaa attc
//
LOCUS       DAPPNJ010000154        10591 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000154 DAPPNJ010000000
VERSION     DAPPNJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.155"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            148..1515
                     /gene="dcuC"
                     /locus_tag="RPM73_003356"
     CDS             148..1515
                     /gene="dcuC"
                     /locus_tag="RPM73_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDX6521687.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVLLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1558..2055)
                     /gene="sspB"
                     /locus_tag="RPM73_003357"
     CDS             complement(1558..2055)
                     /gene="sspB"
                     /locus_tag="RPM73_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDX6521688.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2061..2699)
                     /gene="sspA"
                     /locus_tag="RPM73_003358"
     CDS             complement(2061..2699)
                     /gene="sspA"
                     /locus_tag="RPM73_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDX6521689.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3094..3486)
                     /gene="rpsI"
                     /locus_tag="RPM73_003359"
     CDS             complement(3094..3486)
                     /gene="rpsI"
                     /locus_tag="RPM73_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDX6521690.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="RPM73_003360"
     CDS             complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="RPM73_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDX6521691.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="RPM73_003361"
     CDS             complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="RPM73_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDX6521692.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5470..5868
                     /locus_tag="RPM73_003362"
     CDS             5470..5868
                     /locus_tag="RPM73_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDX6521693.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6022..7389
                     /gene="degQ"
                     /locus_tag="RPM73_003363"
     CDS             6022..7389
                     /gene="degQ"
                     /locus_tag="RPM73_003363"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDX6521694.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7479..8546
                     /gene="degS"
                     /locus_tag="RPM73_003364"
     CDS             7479..8546
                     /gene="degS"
                     /locus_tag="RPM73_003364"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDX6521695.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8608..9546)
                     /gene="mdh"
                     /locus_tag="RPM73_003365"
     CDS             complement(8608..9546)
                     /gene="mdh"
                     /locus_tag="RPM73_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDX6521696.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9981..10451
                     /gene="argR"
                     /locus_tag="RPM73_003366"
     CDS             9981..10451
                     /gene="argR"
                     /locus_tag="RPM73_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDX6521697.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2606 a         2692 c         2638 g         2655 t
ORIGIN      
        1 gcgcatattt tttaatgaaa aatcacctca cctacaatta aaaacacgac atccgcacca
       61 taaatagact tgcaaaaaat ataacatcat tgttttcaat ctgccgttta tgggattgac
      121 cgttttcttt tgacacggag ttcaacaatg ttcggcataa ttatatctgt catcgtatta
      181 attacgatgg gctatttgat cctgaaaaac tacaaacctc aggtgctgct ggctgccgca
      241 ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg gtggtgtact cgatcccgcc
      301 aaaagcagcg gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac
      361 cgcattgccg gattggggct gtcgattatg gcggtgggcg gttatgcccg ctacatggag
      421 cgcacaggag ccagtcgcgc gatggtgagc ttgctaagcc gcccgttaaa actcattcgc
      481 tcgccgtata ttattctgtc ggcaacttac gtcatcggcc aaatcatggc gcagtttatt
      541 accagcgcct ccggcctggg tatgttgctg atggtcacct tatttccgac gctggtgagt
      601 ctgggagtaa gtcgcctctc tgcggtggcg gttatcgcaa ccacgatgtc cattgagtgg
      661 gggattctgg aaacgaactc catttttgca gcccaggtcg cgggaatgaa aattgccact
      721 tacttcttcc actaccagct tccggtcgcc tcttgcgtca ttatctcggt ggcgatctcc
      781 cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa atatcaatca cgaacaggca
      841 gagctaaaag ctctcgataa tgtcccgccg ctctattacg ccattttacc tgtgatgccg
      901 ttaatcttga tgctcggctc gctgttcctc gcccacatcg ggctgatgca gtcagaactg
      961 catctggtgg tggtgatgtt actgagtttg acggtgacga tgtttgttga gttcttccgc
     1021 aagcataact tgcgcgaaac aatggacgat gtgcaggcgt tttttgacgg catgggtacg
     1081 cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa tatttgcgaa aggcttaacg
     1141 acgattggca ctgttgatgc ggttatcagg ggtgcggagc attctggtct gggcggtatt
     1201 ggcgtgatga ttattatggc gctagtcatt gccatttgtg ccattgtgat gggctctggc
     1261 aatgcgccgt ttatgtcatt tgccagtctt attccgaata tcgcagccgg attacatgta
     1321 ccagcggttg taatgattat gccgatgcat tttgccacga cgctagcgcg cgcggtttcg
     1381 ccgattactg cggtggtggt cgttacgtca ggaattgcag gcgtttcgcc ttttgcggtg
     1441 gtgaagcgaa cagcgatccc catggcagtc ggtttcgtgg tgaatatgat tgccacaatc
     1501 acgctatttt attaagccat taaaagacaa aacaggccac cagggcctgt ttttgtatta
     1561 cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt catcgtcagg
     1621 atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa cggtttcgtt
     1681 gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg cagcttcagg
     1741 ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg cagccagcgg
     1801 cacagaaacc tgacgcggaa tgccgccaaa gcgcgcgtta aagcgtacct catcattcgc
     1861 cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc cgtcacgcgc
     1921 atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt gcggcgtgag
     1981 ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac gtggtgttag
     2041 ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc tgcttcagtt
     2101 aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt cagctctttc
     2161 gcacccgggc cgctgaactc gatgcccagt tgcggcagac gccacagcag cggagcaaga
     2221 tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc gaagaccggc
     2281 gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc agaagctgaa
     2341 ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg catgtacaga
     2401 cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg gaaacgctca
     2461 tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc caccagggtc
     2521 ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc cttttccacg
     2581 tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg atggctatag
     2641 atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc gacagccatg
     2701 aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg ccagtcagaa
     2761 gttgtgttac ccagtaagga acgactctct ttgttcgaaa atcaaacaaa aaatgagcaa
     2821 tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga cctttgtggt
     2881 gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag ttgacttagt
     2941 cccttattgg cgatgtggtt tttgttttgc ctgtctgtca gacggtagca aaaagcaact
     3001 ttccaatttt tacgctgatt cagaatttaa ccataaaaaa acccgccgaa gcgggttttt
     3061 tcgcaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact gcggacgacg
     3121 acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac gagtaacgaa
     3181 gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag cgcgggtgat
     3241 accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg tgatgtacag
     3301 gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca tgcgagcagt
     3361 ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac cgttgcccgg
     3421 tttgatgaaa acgcgagctg cggaactttt gcggcgacca gtgccgtagt attgattttc
     3481 agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg ccgcgtggtt
     3541 gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca gcgggccttt
     3601 tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag caatcatctc
     3661 ttcaaaggtc gcttgtttga taccaccgat gtggccggtg tggtgatagt acactttgtc
     3721 agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga tgtaatcacc
     3781 ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac gagccagttc
     3841 agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt cgcgttttac
     3901 ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat agttacacgt
     3961 tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc ggcaaaccta
     4021 ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact ttcttgtaac
     4081 gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt tgtgatttgt
     4141 gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg catctcttgc
     4201 agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata aatttcatac
     4261 agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc atccaccagc
     4321 gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac atcaaacaac
     4381 atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg ggcgtcgacg
     4441 cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt cgccaatggc
     4501 cgatggttga tttctaacgt cggtgaattt tctcgtttcg cccccgccag cgccaaccat
     4561 agtttatcca tctgcgcccg ggtttcatcg ttgagtggcg aaagccacag atgcgcctga
     4621 gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc acaatgctgt
     4681 ttaatggcat cgattgcagg cagaaaacgc gcacgttgca ggccatttcg ataaagttca
     4741 tccggcggaa tatttgacgt cgctaccagg gtaatgccgc gggcgaacag ggctttcatc
     4801 agaccgccaa gtagcatggc gtcggtaata tcagaaacaa aaaattcgtc aaaacagagc
     4861 acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt ctgcccctgt
     4921 aaggcagtta gctcttcgtg cacccgcagc ataaaacggt gaaagtgcag gcgctgtttc
     4981 cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc gcgtcctaca
     5041 ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg tttaccccac
     5101 agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcct gctattgatg
     5161 agttcttgat aaataatttc caggcggctg acggcctctt tttgaacgtc gtcgggttga
     5221 tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct ttgcatgatc
     5281 ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg
     5341 ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg gttatagtgg
     5401 cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa aagacaacgg
     5461 gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat cattattggt
     5521 gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa
     5581 ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag ccactttgcc
     5641 cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta tcagcacatg
     5701 gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat
     5761 cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc tcgcgactat
     5821 tctgaaggtg catccggcct gctgcgtact ggcgcgaagc gcgactaatt tatttttcgg
     5881 gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat ctggtgtctc
     5941 attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt ttcttatcat
     6001 tcaggtacga gagcaggaat aatgaaaaaa caaacccagc tgttgagtgc attagcgtta
     6061 agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc aggccaggtt
     6121 gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct tccggcagtg
     6181 gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga agaattcaaa
     6241 aagttttttg gtgatgattt accggatcaa cctgcacaac ccttcgaagg tttaggttcc
     6301 ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt gattaatcag
     6361 gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa actgattggt
     6421 agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa attaacgcaa
     6481 atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc ggtcggtaac
     6541 ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg acgcagcggg
     6601 ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa ccgcggtaac
     6661 tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac tgcaatcctt
     6721 gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat ggcgcgaaca
     6781 ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg catcaaaggc
     6841 accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt
     6901 gtcagcgaag tgttgccagg ttctggctca gcgaaagcgg gcgtcaaagc gggcgatatt
     6961 attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc tcgtatcgcg
     7021 accaccgagc cgggcacgaa agtgaagctt ggcctgctgc gtaacggcaa accactggaa
     7081 gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat gatcacgcca
     7141 gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa aggtattaaa
     7201 atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa agacgatgtg
     7261 atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg
     7321 gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat ctatctgctg
     7381 atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt aactcgtggt
     7441 atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag ctcttacgtt
     7501 ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca
     7561 gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct gccagttata
     7621 atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt ggtttgaaca
     7681 ccaactctca caaccagctt gagatccgca ccttgggatc cggtgtaatc atggatcaac
     7741 gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct
     7801 tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta accgatctgg
     7861 cggtacttaa aattaatgcc actggcggtt tacctaccat tccgatcaat gcacgtcgcg
     7921 taccgcacat tggcgacgta gtactggcga tcggtaaccc gtacaacctc gggcagacca
     7981 ttacccaggg gattattagt gccacgggtc gaatcggtct gaacccgacc gggcggcaaa
     8041 acttcctaca aaccgatgct tccattaacc acggtaactc tggcggcgcg ctggtgaact
     8101 cgctgggaga actgatgggc attaacacgc tgtcgtttga taagagtaac gatggcgaaa
     8161 cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt atggataagc
     8221 tgatccgcga tggtcgcgtg atccgcggct acattggtat cggcggacgc gagatcgcac
     8281 cactgcacgc gcagggcggt ggtatagatc aactgcaagg gatcgtggtt aatgaagtgt
     8341 cacctgacgg cccagcggcg aatgcgggta ttcaggtcaa cgatctgatt atttcggtgg
     8401 ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa attcgccctg
     8461 gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg caggtcacca
     8521 ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaccg gagtctgtgc
     8581 tccggttttt tattattcgc taatcaatta cttattaacg aactcttcgc ccagggcgat
     8641 atctttcttc agcgtatcca gcataccttc cagcgcgttc tgttcaaatg cgctcagggt
     8701 accgatagat ttacgctctt ccacgccgtt tttacccagc agcagcggtt gagagaagaa
     8761 acgggcgtac tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt gttcgccctg
     8821 cagtgcacga accagagaca gaccaaaacg tgcagctgcc tggcccatag acagggttgc
     8881 agacccgcca ccggctttcg cttcaaccac ctcagtaccc gcgttctgga tacgtttggt
     8941 cagatcagcc acttcctgct cggtaaaact aacgccagga acctgtgaca gcagcggcag
     9001 aatggtaaca ccagagtgac cgccaataac cggcacttca acttcgcctg gctgtttgcc
     9061 tttcagttcc gcaacaaagg tgttggaacg aatgatatcc agcgtggtaa cgccgaacag
     9121 tttgtttttg tcataaacac cggctttttt cagcacttca gcagcaattg caacagtggt
     9181 gttaaccggg ttagtgataa taccaatgca cgctttcggg caggttttcg caacttgctg
     9241 taccaggttt ttcacgatgc cggcgttaac gttaaacagg tcggaacgat ccatacccgg
     9301 tttacgcgct acacctgcag agataagaac gacatctgcg ccttccagcg ccggagtcgc
     9361 atcttcacca gaaaaacctt tgattttcac agcagtaggg atatggctca gatcgacagc
     9421 cacaccggga gtcactggag cgatatcata cagagagagt tctgaacctg aaggcagttg
     9481 ggtttttaac agtagtgcaa gcgcctggcc aataccgcca gcagcgccga ggactgcgac
     9541 tttcatccta aactccttat tatattgata aactaagata tgttgctccg ctgccgcgac
     9601 cttaatccac aaaattgccg tttacaatga ccacatctca agaatgtgta gtcacgcaag
     9661 tttagcgttt atgcatttaa ttgccgtaat caggaaccta acttacgtaa ttaacagtcg
     9721 cttatgcatt agcgcaacat tccaacaggt ggtgacaata taccctaccg ttcagccaaa
     9781 acaacatcaa tttgataaca attaatttac ttttaagcag aatttgcatg ccgtgacgca
     9841 ggcatgtttc tcaataacga aatttgataa aatcccgctc tttcataaca ttatttcagc
     9901 cttcttcagg gctgactgtt tgcataaaaa ttcatctgta tgcacaataa tgttgtatca
     9961 accaccatat cgggtgactt atgcgaagct cggctaagca agaagaacta gttaaagcat
    10021 ttaaagcatt acttaaagaa gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc
    10081 aggagcaagg ctttgacaat attaatcagt ctaaagtctc gcggatgttg accaagtttg
    10141 gtgctgtacg tacacgcaat gccaaaatgg aaatggttta ctgcctgcca gctgaactgg
    10201 gtgtaccaac cacctccagt ccattgaaaa atctggtgct ggatatcgac tacaacgatg
    10261 cagttgtcgt gattcatacc agccctggcg cggcgcagtt aattgctcgc ctgctggact
    10321 cactgggcaa agcagaaggt attctgggca ccatcgctgg cgatgacacc atctttacca
    10381 cccctgctaa cggtttcacc gtcaaagacc tgtacgaagc gattttagag ctgttcgacc
    10441 aggagcttta atctctgccc cgtcgtttct gacggcgggg aaaatgttgc ttatccctct
    10501 caaccccctg ctttcccctg cgattaattt aacgaatagt gcgttttact gcgacatgtc
    10561 attcacacaa tgaatacata aggtaaaaaa a
//
LOCUS       DAPPNJ010000155        10588 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000155 DAPPNJ010000000
VERSION     DAPPNJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.156"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..551
                     /locus_tag="RPM73_003367"
     CDS             <1..551
                     /locus_tag="RPM73_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDX6521698.1"
                     /translation="PERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGWYHKAPE
                     LHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLFAIGISG
                     YLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHGFMALKH
                     HFIDKDDTLKRMLGKSSSDYGV"
     gene            555..1130
                     /locus_tag="RPM73_003368"
     CDS             555..1130
                     /locus_tag="RPM73_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDX6521699.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1172..2224)
                     /locus_tag="RPM73_003369"
     CDS             complement(1172..2224)
                     /locus_tag="RPM73_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDX6521700.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGVPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2449..3369
                     /gene="lpxL"
                     /locus_tag="RPM73_003370"
     CDS             2449..3369
                     /gene="lpxL"
                     /locus_tag="RPM73_003370"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDX6521701.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3541..4767
                     /gene="mdtG"
                     /locus_tag="RPM73_003371"
     CDS             3541..4767
                     /gene="mdtG"
                     /locus_tag="RPM73_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDX6521702.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELVGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4850..5224
                     /locus_tag="RPM73_003372"
     CDS             4850..5224
                     /locus_tag="RPM73_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDX6521703.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5225..5452)
                     /locus_tag="RPM73_003373"
     CDS             complement(5225..5452)
                     /locus_tag="RPM73_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDX6521704.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5625..8168)
                     /gene="mdoH"
                     /locus_tag="RPM73_003374"
     CDS             complement(5625..8168)
                     /gene="mdoH"
                     /locus_tag="RPM73_003374"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDX6521705.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8161..9696)
                     /gene="mdoG"
                     /locus_tag="RPM73_003375"
     CDS             complement(8161..9696)
                     /gene="mdoG"
                     /locus_tag="RPM73_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDX6521706.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10090..>10588
                     /locus_tag="RPM73_003376"
     CDS             10090..>10588
                     /locus_tag="RPM73_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDX6521707.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLEN"
BASE COUNT         2609 a         2768 c         2610 g         2601 t
ORIGIN      
        1 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
       61 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
      121 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
      181 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
      241 ctcgtcttgc cgcaaaggct ggacatctcg cattgtacct attgctgttc gccatcggca
      301 tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg
      361 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
      421 tttggctcgc gtggagcgtc gtagtactgt ccgttatgca cggatttatg gccctgaagc
      481 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
      541 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
      601 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
      661 ttaatttccg catccagcac cttggctata gctggttata cggcaccttt aaagatttcg
      721 acggtacttt tacctttgac gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
      781 acaccaccag cgtcgatact aatcacgccg aacgcgataa acatcttcgc agtgcagatt
      841 tcctcaatac cgcaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
      901 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
      961 tggaagcgaa attaattggt cagggcgacg acccatgggg tggtaaacgt gcaggcttcg
     1021 aggccgaagg caaaattaag ctcaaggact tcaatatcaa gacagattta ggtccagctt
     1081 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
     1141 ttgccggatg gcacgcatcc ggcaatgata tttattccgt tggatcagga atgcccagtg
     1201 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
     1261 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
     1321 aacctttgta tttttccgca catactggac actgaataaa cagcaggtgg cagccatcat
     1381 ttttacagtt ggtatggctg tcgcacggca cgccgcactg gtggcaatgc gcgataatct
     1441 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
     1501 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
     1561 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
     1621 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
     1681 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
     1741 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
     1801 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
     1861 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaaa gatttcccgt
     1921 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
     1981 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
     2041 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
     2101 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
     2161 cgctctcagc caacattttg gcttttagcg cgtcgttgga aatgcggttg tgtaacactg
     2221 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
     2281 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
     2341 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
     2401 tgcgacaata catacaattg cccgaatagg ttgaaaaaca ggattgatat gacgaatcta
     2461 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
     2521 gtactttggt tagtcgtgca attgccctac ccggttatct accgcctcgg ttgtggatta
     2581 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
     2641 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
     2701 tccgttggca tgggcctgat ggaaaccggc atggcgtggt tctggccgga ccgccgaatc
     2761 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
     2821 ggcatcctgt tagttggcat ccattttctg acactggagc tgggtgcgcg gcagtttggt
     2881 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
     2941 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
     3001 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccggatca tgattacggc
     3061 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
     3121 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
     3181 ccagatggca aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat
     3241 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
     3301 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
     3361 cgctattaaa tctcccctgc cggatgcttc agaatggcat ccggcattac cacagcaaat
     3421 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
     3481 tagtgtgctt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
     3541 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
     3601 ctaggctgtt ttcttaccgg tgccgccttc agtctggtaa tgcccttcct acccctctac
     3661 gttgagcagc ttggagttac cggtcactcc gccctgaata tgtggtccgg tattgtcttc
     3721 agcattacat ttttattttc ggccatcgcc tcaccgtttt ggggtggact cgccgaccgt
     3781 aaaggccgaa aactcatgct attacgctct gccctcggca tgggcatcgt gatggtgttg
     3841 atgggactgg cacaaaatat ctggcagttt ttgatcctac gggcgcttct tgggttactt
     3901 ggcggatttg tccccaacgc taatgctctt atcgccacac aagtaccgcg taataaaagc
     3961 ggctgggcgc tgggtacgct ctccacaggc ggcgttagtg gtgcgttgct cggcccaatg
     4021 gctggcggcc tgctcgccga tagctacggc ttacgtccgg tattctttat taccgccagt
     4081 gtgctcatac tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt ccagccggtc
     4141 agcaaaaaag agatgctgca catgcgggaa gtggtgacat cacttaaaaa cccgaaactg
     4201 gtactcagcc tgtttgtcac cacgttaatc atccaggtgg cgacaggctc aattgccccc
     4261 attctgacgc tgtatgtccg cgaactggtg ggtaacgtca gtaacgtcgc ctttatcagt
     4321 ggcatgatcg cctcggtgcc aggcgtggcg gctctgctaa gcgcaccacg actcggcaaa
     4381 cttggcgatc gaatcggacc cgaaaagatc ctgattacag cgctgatctt ttctgtactg
     4441 ctgttgatcc caatgtctta cgttcagacg ccattgcaac ttgggatttt acgttttttg
     4501 ctcggtgccg ccgatggtgc actactcccc gccgtacaga cactgttggt ttacaactcg
     4561 agcaaccaga tcgccgggcg tatcttcagc tataaccaat cgtttcgtga tattggcaac
     4621 gttaccggac cattgatggg agcagcgatt tcagcgaact acggtttcag agcggtattt
     4681 ctcgtcaccg ctggcgtagt gttattcaac gcagtctatt catggaacag tctacgtcgt
     4741 cgtcgaatac cccaggtatc gaactgattt ttcgcctttc atacttgcaa aagcggagaa
     4801 tcagctatcc ttttccctga aacctcatca actcaaaggg agaatcgtga tgaccatgta
     4861 cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag acaaccccgg
     4921 catcgacgcc gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg ttttgcagga
     4981 cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag gtgaatgttt
     5041 acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg atgagataga
     5101 gatacgccag gagtggcagg aagagaatac attacatgaa tgggacgagg gggaatttca
     5161 gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt gggatgaacg
     5221 ttaatcactc atacgggcca tgatgaatgt cgatcggcag cagtaaagta tcaaaaacca
     5281 gagagaatgg cagatcaagg atcgtgacat aacgccaggc ggagtcacgc acatcccatt
     5341 gcacaccggg ataatattgg ttgccatgcc cctgccccgg tatagtgcga ctaataatgc
     5401 tgccacagcc actcagcaag gtcaccatga tgctcaccac aattaaacgc atttgatacc
     5461 tctttcgtaa aaaaataccg gcataacgcc ggtattttta ttggctcgtt gtttttgccg
     5521 gatgcggcgt gaacgcctta tccgacccac aaaatcttgc aaaatcaata aattgcagga
     5581 acgatgtagg cctgataagc gtagcgcatc aggcaactac gtttttattg cgaagccgca
     5641 tccggtttac gcaatgccag tggattgagc tttatccctt cgtaataact cacccatgaa
     5701 gaatatctct ccggggaatt ccagacgcgg aaatgcagac gggccatcgt caccggatcg
     5761 cttagcagca ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt cagcgcctgt
     5821 tcaacgtggc ggtcacgggc gatttccagc accttactgg cgcggtgacg cgcggtcgcc
     5881 attgcggttg ccagagcgtt aaatgacggg ttaaacactg cgtgcataaa gccatcatca
     5941 agggagcgtt gacgattcat ctcaaggaac cgatcggtat caaccagcac ctgcggcggc
     6001 gaatactctt ccgggatcag gaacagtttc cagcgtttgg tgcgcagacc aacggtggca
     6061 cggctggaaa tcaccgaaac aaacggtgac aggatcaacg agaagacaat cggtgccagc
     6121 cagaacagga aacgcagatc cagccacgcc atcccaacag cccacactaa ccctaacagc
     6181 agctgtgagc cgtggcgttt gaacgcttca ccccaggaag tggagtcatc atcacgctgc
     6241 ggtgaattcc acaccacttc ccagccaagg aacgcgctga caacgaagac cgtatggaac
     6301 agcatgcgta ccggagccag cagcacggaa aaaagcactt ccagcagcaa cgataatgta
     6361 acgcgccaga agccgccgta ttctttcgtt cctttgcacc agataagcaa aatgctcaat
     6421 aacttcggca ggaacaacag caccatagtc gaagcaaaaa gtgcaatcgc cagctcagga
     6481 cgccactgcg gccacaccgg gaacaactgc cgtggttgca ggaagtattg cggttcggtc
     6541 aacgcatgta ctacctgcaa tgcagtagag agcgcgagga acataaacca cagcggagcg
     6601 gagagataag acatcacgcc cgtcaggaat accgcacggt gaaccgggtg catacccttc
     6661 accaggaaca gacggaagtt catcaggtta ccgtggcacc agcggcggtc acgttttagc
     6721 tcatcaagca agttaggcgg caattcttca taagaacccg ggagatcgta agcaatccag
     6781 accccccaac ctgcacggcg catcaacgcc gcttccacga agtcatgtga caggattgaa
     6841 ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc agtgctcgat aaacggtttc
     6901 acgcggataa tcgcgttatg tccccagtag tgcgactcgc caagttgcca gaagtgcaaa
     6961 ccggctgtaa acagtggccc atacacgcgg gtcgcgaact gctgacagcg cgcatacagc
     7021 gtatccatac cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc
     7081 atcaagcgca ccaggccgca taaacaatca ccggtcatta ccgagtcagc atccagcacc
     7141 accatgtagc tgtactggct gccccagcga cggcagaagt catcgatatt accgctttta
     7201 cgcttcacgc ggcgacggcg gcggcgatag aaaatctgac cttcgccacc gacttcagcg
     7261 ataagctcca tccaggcttt ttgctctgcg acgcagatat ccgggttata actgtcacta
     7321 agaatgtaga catcaaagtg tttggcattc ccggtggctt ttactgattc ccacgttgca
     7381 cgcaggccag caaaaacacg gttcacgtct tcgttacaga taggcatgat caacgccgtg
     7441 cgatgctccg ggtttaatgg ttcatcgcca actgttgacg cagatatact gtatttatcg
     7501 cgaccaataa gcagttgcag gaagcccatt aacgccgtcc agaatccggc ggacacccaa
     7561 cagaacagta ccgcaaagag gatcaggata ccggtttgca gcatataagg cagaagctgc
     7621 ataaaggaaa cccacaaatc ctgaccaacc atatccatag gattaatcag cgcccaaccc
     7681 tgataaggaa gaatggtctt catataccag gtcgcgacga cagtttgcgc gagcgtcagg
     7741 atcaacagaa tgtaacggcg gatggtaccg acggtacgcc acttttgctc gctctcctgc
     7801 tcttctttgg tcaaacgagc cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc
     7861 cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa acatcgagga gcgttttgct
     7921 tctggcatcg ccttcagctg atcgcgccct tcgtcgtctt taattaactg tccatcagca
     7981 agtgaatctg gccaggcttg ttccagacgc gcctttaccg agccttgcgg ggaatcatcc
     8041 tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc
     8101 ttcggcaatg ccgctttctc gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc
     8161 ttattcattg gcaggtaact ggtagctcca ggtttcactc aacgtctgat cggcattcac
     8221 cagcgcagca cgcatttcag tggttttctt ggcatctttc actttcacac gcatcaccag
     8281 acgccagcct ttggtaaccg ggttatagcg caccgtgctt tcaactatct caccattatc
     8341 accaatgctg gtttgcgctg tgaccggggt atcctctggc agttttttca tctctgcgcc
     8401 ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt
     8461 cacatccccc gttgaacgac gcgtttgttg cacccatgcg ttatctggcg catgcagttt
     8521 gtcttcatca cggctgaagg tgatggtgta tttaaagttc atctctttac ccggctccgg
     8581 cagctgatcc ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt tggttggaat
     8641 ttccaccagc tcaacgctgc ctttacccca ctcccctttc ggagtcaccc atgcgcttgg
     8701 acgaagatcg taacgatcat cgagatcttc aaagcgggag aaatcacgac cgcgctgcaa
     8761 cagaccgaag ccttgcgggt tctccatcga gaagctgctg accgctaaat gtttcgggtt
     8821 attcaacgga cgccagatcc attcgccatt accggcatgg atagacagac cgttagagtc
     8881 gtgcaactcc ggacgatagt tatttgcagg cgacggttgg ttcggcccaa acaggaacat
     8941 actggttaac ggtgcaaccc ccagtttgcc gactttatcg cgcagataga ttttcgactg
     9001 cacatccaca accgtgtcac gccctggcat aaccacgaat ttgtaagcac ctgtcgcgcg
     9061 cggcgagtca agcaatgcat aaatggttaa acgtttatca gtcggttttg gacgctcgat
     9121 ccagaactct ttgaagcgtg gaaattcttc acccgatggc aaggcggtat caattgccag
     9181 gccgcgggca gaaaggccat aaacctgacc tgcaccaatc acgcggaaat agctggcccc
     9241 gagcatgctg acgatttcat cgtttttatc tttgctgttg atcgggtaaa gcactttaaa
     9301 accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa
     9361 ataatccggg ctgtatttga ttcgtttgac tgcggtggca gtcacttcat ttattttgac
     9421 cggggtatcg aagtacatac cctgatggta gaactcgagt ttgaatgggg tcttcagatt
     9481 gttccagtac gctttgtcat gattaaactg gatctgctga tagtccgcgt atttcatatc
     9541 gcggaaaacg gagggcaagt tgcttttggg cgcctcatag cctttcccgg ctaaggattg
     9601 agcttgcttt gcgacatcat caatactgaa agcccagctt gaagatgtat acagggttaa
     9661 cattactgca gcactcaacc aacgcatttt catcatttgt agtttatgtt tcataattag
     9721 taagcacttc cccctttgtg tgcttatatc gatccgatct attttaatgg ataaccagcc
     9781 attccgacaa cagaatcccg gtatcgttca gcacgctggc tcatggatta agcacctgat
     9841 tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga agcatcgact tcgtgtaggg
     9901 tttgccagca attttattta tcaaccgtct tacagacgct tccattctgg ttagtggtag
     9961 cgatgatgct atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc
    10021 tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt
    10081 taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc
    10141 tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg
    10201 catgtgaata gcgccgaacc atcattgtgg ctgacccttt ttaatgactt catccactcg
    10261 ttccgcatgc aggtattttt cgttatatcc ggctactttt cctacatgct ttttttacgc
    10321 tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca
    10381 gccatccccc tactgacatt accgcaattt attatgctgc aatatgtcaa aggaaaagcg
    10441 gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata
    10501 tcacacctgt ggtttttact ggtgttagta gtcatgacga cgctgtgcgt atggatattt
    10561 aagcgcatca gaaataattt agaaaatt
//
LOCUS       DAPPNJ010000156        10389 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000156 DAPPNJ010000000
VERSION     DAPPNJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.157"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            40..615
                     /gene="ssuE"
                     /locus_tag="RPM73_003377"
     CDS             40..615
                     /gene="ssuE"
                     /locus_tag="RPM73_003377"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDX6521708.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            608..1567
                     /gene="ssuA"
                     /locus_tag="RPM73_003378"
     CDS             608..1567
                     /gene="ssuA"
                     /locus_tag="RPM73_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDX6521709.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1564..2709
                     /gene="ssuD"
                     /locus_tag="RPM73_003379"
     CDS             1564..2709
                     /gene="ssuD"
                     /locus_tag="RPM73_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDX6521710.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2720..3511
                     /gene="ssuC"
                     /locus_tag="RPM73_003380"
     CDS             2720..3511
                     /gene="ssuC"
                     /locus_tag="RPM73_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDX6521711.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3508..4275
                     /gene="ssuB"
                     /locus_tag="RPM73_003381"
     CDS             3508..4275
                     /gene="ssuB"
                     /locus_tag="RPM73_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDX6521712.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4482..7094)
                     /gene="pepN"
                     /locus_tag="RPM73_003382"
     CDS             complement(4482..7094)
                     /gene="pepN"
                     /locus_tag="RPM73_003382"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDX6521713.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7360..8562
                     /gene="pncB"
                     /locus_tag="RPM73_003383"
     CDS             7360..8562
                     /gene="pncB"
                     /locus_tag="RPM73_003383"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDX6521714.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8731..10131
                     /gene="asnS"
                     /locus_tag="RPM73_003384"
     CDS             8731..10131
                     /gene="asnS"
                     /locus_tag="RPM73_003384"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDX6521715.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2347 a         2688 c         2798 g         2556 t
ORIGIN      
        1 tgttaacaaa aaaagaacaa ttggttataa ggagagagta tgcgtgtcat caccctggcg
       61 ggtagtcctc gctttccttc tcgctccagc tccttgctgg aatatgcgcg ggaaaaacta
      121 aatggcctcg atgtagaggt ttatcactgg aatctgcaaa acttcgcccc ggaagatctg
      181 ctttatgctc gtttcgatag tccggcactc aagaccttca ccgaacagct gcaacaggct
      241 gatggactga tagtcgctac acctgtgtat aaagccgcct attccggcgc gctgaaaacc
      301 ctgctcgacc tgctgccaga gcgcgctttg caaggcaaag tggtgctacc gctggcgacg
      361 ggcggtaccg tggcccatct gctggcggtc gattatgccc ttaaaccagt tttaagcgca
      421 ctgaaagctc aggagatcct gcacggcgtg tttgccgatg actcacaagt aattgattac
      481 catcacagac cccagttcac gccaaatctg caaacccgtc ttgataccgc gctagaaact
      541 ttctggcagg cattgcaccg ccgcgatgtt caggttcctg accttctgtc tctgcgaggt
      601 aatgcccatg cgtaacatca ttaaactggc gctggtggga ttgcttagcg tctctacgat
      661 tgcggttgct gcagaatcct cgcctgaagc gttacgtata ggctatcaga aaggcagtat
      721 tggtatggta ctggcaaaaa gccaccagtt actggaaaaa cgctatccgc aatcaaaaat
      781 ctcttgggtg gagttccccg cgggtccgca aatgttggaa gcgttaaacg ttggcagtat
      841 tgatctcggc agtaccgggg atattccgcc aatctttgcc caggctgccg gggctgattt
      901 ggtatatgtg ggcgtcgagc caccgaagcc caaagccgaa gtgattctgg tggcagaaaa
      961 cagcccgatc aaaaccgtag ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc
     1021 cagttcacac aaccttttac tgcgtgcact gcgtcaggcc ggacttaagt ttaccgatat
     1081 ccaacccact tacctgacgc ccgctgatgc ccgcgccgcg ttccagcaag gtaacgttga
     1141 cgcctgggct atctgggatc cctactactc tgctgcatta ttacagggcg gcgtgcgggt
     1201 gctgaaagac ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtcccta
     1261 tgcagaaaaa aacggcgctt ttattcaggg cgtactcgca acctttagtg aggccgatgc
     1321 gttaacccgc agccagcgcg agcaaagcat cgctttactg gcaaaaacga tgggcttacc
     1381 ggcaccggtg attgcctctt acttagatca tcgccctcct accaccatca aaccggttaa
     1441 cgccgaggtt gccgccttac agcagcaaac ggcagatctg ttttatgaaa atcgtctggt
     1501 gccgaaaaaa gtcgatattc gccagcgcat ctggcagccc actcaactgg aaggaaaaca
     1561 attatgagtc tgaatatgtt ctggttttta ccgacccatg gtgacgggca ttatcttgga
     1621 acggaagaag gttcacgccc ggttgatcac ggctatctgc aacaaattgc gcaagcggcg
     1681 gatcgtctgg gctataccgg tgtgctgatc ccaacggggc gatcgtgtga agatgcgtgg
     1741 ctggtggcgg cgtcgatgat cccggtgacg cagcggctga agtttcttgt cgccctgcgc
     1801 cccagcgtaa cctcacctac cgttgccgct cgccaggccg ccacgcttga ccgtctctca
     1861 aatggtcgtg cgttgtttaa tctggtcaca ggcagcgatc cacaagagct ggcaggcgac
     1921 ggcgtgttcc ttgatcatag cgagcgctac gaagcctcgg cggaatttac ccaggtctgg
     1981 cggcgcttat tgctggggga aaccgtcaat ttcaacggta aacatattca tgtgcgcgga
     2041 gcaaaactgc tcttcccgcc gattcaacag ccttatcctc cgctttactt tggcggatcg
     2101 tcagacgtcg cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa
     2161 ccgccagaac tagtaaaaga gaagatcgaa caagtgcggg cgaaagctgc cgcgcatgga
     2221 cgcaaaattc gtttcggtat tcgtctgcat gtgattgttc gtgaaactaa cgacgaagcg
     2281 tggcaggccg ccgagcggtt aatctcgcat cttgatgatg aaactatcgc caaagcacag
     2341 gccgcattcg cccggacgga ttccgtagga caacagcgaa tggcggcgtt acataacggc
     2401 aagcgcgaca atctggagat cagccccaat ttatgggcgg gcgttggctt agtgcgcggc
     2461 ggtgccggaa cggcgctggt gggcgatggt cctacggtcg ctgcgcgaat caacgaatac
     2521 gccgcgcttg gcatcgacag ctttgtgctt tcgggctatc cgcatctgga agaagcgtat
     2581 cgggtcggcg agttgctgtt cccacatctg gatgtcgcca ttccggaaat tccccagccg
     2641 cagccgctga atccgcaagg cgaagcggtg gcgaatgatt ttatcccccg taaagtcgcg
     2701 caaagctaag gaagaaataa tggcaacgcc agtgaagaag tggttattgc gcgttgcccc
     2761 ctggttttta ccggtgggca tcgtggcggt gtggcaactg gcctcctcag ttggctggct
     2821 gtcgacgcgt attttgccct caccggaagg ggtggtgacg gcgttctgga cgctctccgc
     2881 cagcggcgaa ctgtggcagc atctggcgat cagttcctgg cgggcgctga ttggcttttc
     2941 aattggcgga tcgctggggt tgattttggg actgattagc gggctatctc gctggggaga
     3001 acggctgctg gatacctcaa ttcagatgtt gcgcaacgta ccgcatctgg cgctgatccc
     3061 gctggtgatt ttgtggtttg gcattgatga atccgcaaaa atctttctgg tggcgctcgg
     3121 cacgctattc cccatttata tcaacacctg gcatggcatc cgtaatatcg atcgcggtct
     3181 ggtggaaatg gcgcgtagct atggattatc cggcataccg ctgtttatcc atgtgatcct
     3241 gcctggtgcc ctgccctcaa ttatggtcgg cgtgcgtttt gcgttgggcc tgatgtggct
     3301 gacgctgatt gttgccgaaa ccatttctgc caattcaggc attggttatc tggcgatgaa
     3361 tgcgcgggag tttttgcaaa cggacgtggt ggtagtcgct attattcttt acgccctgct
     3421 cggcaaactt gctgacgtca gcgcgcagct gttagagcgc ctctggttgc gctggaaccc
     3481 ggcttatcat ttgaaggagg ccacggtatg aatactgctc gtctgaacca gggcacacca
     3541 ttgttgctca atgcagtaag caaacattac gcggaaaata tcgtcctgaa ccaactggat
     3601 ttacatattc cggcaggtca gtttgtggcg gtggtgggcc gcagcggtgg tggcaaaagt
     3661 accctgctgc gcctgctggc cgggctggaa acgccaaccg caggcgatgt gttggcaggc
     3721 accacaccgc tggctgaaat tcaggaagac acgcgaatga tgtttcagga tgcgcgtctg
     3781 ctaccgtgga aatcggtgat tgataacgtt gggttaggcc ttaaaggcca gtggcgcgat
     3841 gccgcgcgtc gggcgttggc tgcggtagga ctggagaatc gcgccgggga atggcctgcc
     3901 gcactttcag gcgggcagaa acagcgagtg gcgctggcaa gagcgttgat tcatcgaccg
     3961 ggtttattgt tgcttgatga accgctcggg gcactggacg ccttaacgcg actcgagatg
     4021 caggatttga ttgtgtcact ttggcaggag cacggcttta ccgtactgtt ggtgacgcat
     4081 gatgtgagcg aagcggtagc aatggctgac cgggtgttgt taattgaaga gggaaaaatt
     4141 ggtctggatt tgacagtgga tatcccccgt ccgcgtcgat tagggtcggt gcgattagcg
     4201 gagctggaag cggaagtatt gcaacgggta atgcggcgtg ggcattctga acaactgatt
     4261 cggcgtcatg gttaatatgc cggatgtggc gcacaaaacc agcattttca acggactagt
     4321 aggcctgata agacacgtca agcatcgcat caggcagttg ggcacggctg ccggatgcgg
     4381 cgtgaacgcc ttatccagcc tacaaaacca gcattttcaa cggactagta ggcctgataa
     4441 gacgcgtcaa gcgtcgcatc aggcattttg gctcaaaagt atcaagccag tgctttggtg
     4501 atcttctcgt acagatcgcc agagagattt tccagccctt tcaactgttc cagcgccgcg
     4561 cgcattttct cctgacgttt ggcatcataa cgtttcaggc gaatcagcgg ttcaatcaga
     4621 cgtgaagcta cctgcgggtt acggctgttg aggtcggtaa gcatttccac caggaactgg
     4681 taaccgctgc catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc
     4741 aacgaacgaa tacggttcgg gttgctcatg gtaaatgagc gatgctgcaa cagaccacgc
     4801 accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc
     4861 accagaccgt cctgatgcca cttatcgtcg tactcctgca tcagcgcgtc acggcaaggc
     4921 agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca
     4981 tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc
     5041 aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacacg gtactcgctc
     5101 tggtaattcg cgttgtaaat agccagcagc tcatccgcca gttcagtcgc cagagtacga
     5161 gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc
     5221 atttcattga cagaaggcag cgtcaggatt tctgccgcca gcgccggatc aatcttctca
     5281 tcgagcagca ccgcgcggaa agcgtcagcc acatgtaccg gcagagacag cggctgccct
     5341 tgctgatgac gggcgacgtt cagcttgatg taggttgcca gcaaactttg cgccgcatcc
     5401 caacgggaga aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac
     5461 ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag
     5521 tagacattat caaaaacaaa ggtctgttcc gcctgggtga cgttcagcac ggaattcacc
     5581 ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tgtcatacaa ttcgatggca
     5641 aatggaatat gcagcggctg tttttctgcc tgatccggcg tggctggcgt gcgctggctg
     5701 atggtcaggg tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt cacaatcggc
     5761 gtgccggact ggctgtacca acggcggaaa tgggagagat cgacattcga cgcatcttcc
     5821 atcgcctgca caaagtcgtc gcaggttgcc gcgctgccat catggcgctc gaaatagagt
     5881 tgcatccctt tctggaagtt ttcttcgccc agcagggtgt ggatcatgcg aatcacttcc
     5941 gcgcccttct cgtaaacggt cagggtgtag aagttgttca tctcaatgac catatccggg
     6001 cggatcgggt gcgccatcgg gctggcatct tccgcaaact gcaatccgcg catggtgcgc
     6061 acattattga tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg
     6121 aagacggtta aaccttcttt caggctgagc tggaaccagt cgcggcaggt cactcggtta
     6181 ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag ataatctttg
     6241 tcggtggcgg tgtcggtgcg ggccaacaca tatttggagt taaagatatt cagcccctta
     6301 ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca
     6361 tactccaggc caaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc
     6421 cacggcgcgc gatcaaggtt gccgcgatcg acgtacagct ccagtgctac ttcgcgacca
     6481 gaacgcgtgg taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg
     6541 tagcacggtt tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt ttcgagttcg
     6601 ccttgcgcaa cgcggttacc attggaaagc aggaagggat atttgatttt atcggcaata
     6661 attttggtgg taaaacgcgc cagcacgtcc gggcggtcga gataatacgt aatatggcgg
     6721 aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc
     6781 gccgtattcg ccgccgggct tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa
     6841 ttactgatga ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc atcattaata
     6901 tgaacagaaa ccagtttgag gtcttcgccg ttgagacgaa gcggagcatc tgatgcacca
     6961 tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc
     7021 aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt
     7081 ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc
     7141 ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg
     7201 ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg
     7261 atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt
     7321 gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct
     7381 gttctgcact cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt
     7441 catcactatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg
     7501 ggtatttatg ccgatgctat tcgtgaacag attcaggcga tgcagcacct gcgcctgcag
     7561 gatgatgaat atcagtggct ctctgctctg cctttcttta aggccgacta tcttaactgg
     7621 ttacgcgagt tccgctttaa cccggaacaa gtcaccgtat ccaacgataa tggcaagctg
     7681 gatattcgtt taagcggccc gtggcgtgaa gtcatcctct gggaagttcc tttgctggcg
     7741 gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc
     7801 gacacgctgg aaagcaaatt agtcgacttc tcggcgttaa ccgccggcct tgatatgtcg
     7861 cgcttccatc ttatggattt tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc
     7921 atcgttaagc gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg
     7981 cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcacatcag
     8041 caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagaa
     8101 tatcccgacc aacttggcat tgcattaacc gactgcatca ctatggatgc tttcctgcgt
     8161 gatttcggtg tcgagttcgc cagtcgctat cagggcctgc gtcatgactc tggcgacccg
     8221 gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tgggaattga tccacagagt
     8281 aaaactctgg ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac
     8341 ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc
     8401 caggtaaaac ccctgaatat tgtgattaag ttggttgagt gtaacggtaa accggtggcg
     8461 aaactttctg acagccctgg caaaactatc tgccacgata aagcgtttgt tcgggcgctg
     8521 cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat
     8581 cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg
     8641 tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta
     8701 ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc
     8761 cagggccgcg tagccgttga cagcgaagtc accgtacgcg gatgggtacg tacccgccga
     8821 gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta
     8881 caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg tctgaccacc
     8941 ggctgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt
     9001 gaaattcagg ccagcaaagt tgaagttgct ggttgggttg aagatccaga cacttacccg
     9061 atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc
     9121 acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc
     9181 ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattaccgc atctgatacc
     9241 gaaggtgcag gcgaaatgtt ccgcgtttct acgctggatc tggaaaacct gccgcgtaac
     9301 gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta
     9361 tctggccagt tgaacggcga aacctacgct tgcgcattat ccaaaattta taccttcggc
     9421 ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg
     9481 gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga agccatgctg
     9541 aaatatgtct tcaaagcggt tctcgaagaa cgcgcagacg acatgaaatt cttcgctgaa
     9601 cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaggccga tttcgcgcag
     9661 gtggattaca ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg
     9721 gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt
     9781 aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctc
     9841 aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat cggtgagatc
     9901 attggtggct cccagcgtga agaacgtctg gacgttctgg acgagcgtat gctggaaatg
     9961 ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg
    10021 cattcaggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg cgtgcaaaac
    10081 gtgcgtgatg tgattccgtt cccacgtact ccgcgcaacg ccagcttctg attttcttcc
    10141 tcaaagagcc agcgcctgct ggctcttttt tatcctttct gtcaagttat ctgtttgtta
    10201 agtcaagcaa tctatttgca accccgccat aacgatcatc ctgttacgga atattacatt
    10261 gcaacattta agcacaaaaa ataacccgca ttcttattgc ggattagttt tttcttagct
    10321 gatagcacaa ttttcatact attttttggc attctggatg tctgaaagag gattttgtgc
    10381 caggtcgat
//
LOCUS       DAPPNJ010000157        10242 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000157 DAPPNJ010000000
VERSION     DAPPNJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.158"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(163..238)
                     /locus_tag="RPM73_003385"
     tRNA            complement(163..238)
                     /locus_tag="RPM73_003385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Lys,seq:ttt)
     gene            complement(242..317)
                     /locus_tag="RPM73_003386"
     tRNA            complement(242..317)
                     /locus_tag="RPM73_003386"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(282..284),aa:Val,seq:tac)
     gene            complement(468..543)
                     /locus_tag="RPM73_003387"
     tRNA            complement(468..543)
                     /locus_tag="RPM73_003387"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Lys,seq:ttt)
     gene            complement(546..621)
                     /locus_tag="RPM73_003388"
     tRNA            complement(546..621)
                     /locus_tag="RPM73_003388"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(586..588),aa:Val,seq:tac)
     gene            complement(757..832)
                     /locus_tag="RPM73_003389"
     tRNA            complement(757..832)
                     /locus_tag="RPM73_003389"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Lys,seq:ttt)
     gene            complement(997..1788)
                     /gene="cpoB"
                     /locus_tag="RPM73_003390"
     CDS             complement(997..1788)
                     /gene="cpoB"
                     /locus_tag="RPM73_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDX6521716.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1798..2319)
                     /gene="pal"
                     /locus_tag="RPM73_003391"
     CDS             complement(1798..2319)
                     /gene="pal"
                     /locus_tag="RPM73_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDX6521717.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2354..3646)
                     /gene="tolB"
                     /locus_tag="RPM73_003392"
     CDS             complement(2354..3646)
                     /gene="tolB"
                     /locus_tag="RPM73_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDX6521718.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3779..5044)
                     /gene="tolA"
                     /locus_tag="RPM73_003393"
     CDS             complement(3779..5044)
                     /gene="tolA"
                     /locus_tag="RPM73_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDX6521719.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5109..5537)
                     /gene="tolR"
                     /locus_tag="RPM73_003394"
     CDS             complement(5109..5537)
                     /gene="tolR"
                     /locus_tag="RPM73_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDX6521720.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5541..6233)
                     /gene="tolQ"
                     /locus_tag="RPM73_003395"
     CDS             complement(5541..6233)
                     /gene="tolQ"
                     /locus_tag="RPM73_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDX6521721.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6230..6634)
                     /gene="ybgC"
                     /locus_tag="RPM73_003396"
     CDS             complement(6230..6634)
                     /gene="ybgC"
                     /locus_tag="RPM73_003396"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDX6521722.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6784..7077)
                     /gene="ybgE"
                     /locus_tag="RPM73_003397"
     CDS             complement(6784..7077)
                     /gene="ybgE"
                     /locus_tag="RPM73_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDX6521723.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7077..7190)
                     /gene="cydX"
                     /locus_tag="RPM73_003398"
     CDS             complement(7077..7190)
                     /gene="cydX"
                     /locus_tag="RPM73_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDX6521724.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7205..8344)
                     /gene="cydB"
                     /locus_tag="RPM73_003399"
     CDS             complement(7205..8344)
                     /gene="cydB"
                     /locus_tag="RPM73_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDX6521725.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8360..9928)
                     /gene="cydA"
                     /locus_tag="RPM73_003400"
     CDS             complement(8360..9928)
                     /gene="cydA"
                     /locus_tag="RPM73_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDX6521726.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2498 a         2788 c         2452 g         2504 t
ORIGIN      
        1 actgagctaa cgacccatac gggtgttacc tgaaagattt ctactcgggg ccaatcttta
       61 agattggtgt gtcgtgcagg atgactcgct tcgcttgccc tacgggccgt tgctggcgca
      121 acgttatcct tcacgcttaa catctgagtt tgatgttaaa attggtgggt cgtgcaggat
      181 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccga
      241 gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
      301 agctgagcta atcacccgat actgcgctgg atactacaaa ctacttccac cgaacccacc
      361 gtaattagtg gtgggtcgtg caggatgact cgcttcgctc gccctacggg ccgttgctga
      421 cgcaacgtta tcctacacgc ttaacatctt gtcttcgatg ctaaaactgg tgggtcgtgc
      481 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
      541 cccggtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      601 tcccagctga gctaatcacc caatactacg ctggatacta caactaacta tgttagtggt
      661 gggtcgtgca ggatgactcg acttcgtctc gcccttcggg ccgttgctga tgcaacgtta
      721 tccttcacgt tttactaccg cttccacctt agcgattggt gggtcgtgca ggattcgaac
      781 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccactttttc
      841 gttgctttcg gtttgtttga tatcccgtgg caacggcggc atatattact gatttcagac
      901 ttgagcgcaa caaaaatttc gatgcagatc actcaactgc ttatgattcg cacgacacga
      961 ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg
     1021 tgcctgttta gcgccatcag taccagggta tttactgata acctgctggt atacggcttt
     1081 cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg actttaaaca tcgcatcagc
     1141 agcctttggt gacttcggat agtttttcac taccgaagca aaatagtacg ccgcatcatc
     1201 ttttttaccc ttgttgtagt ttaactgacc cagccaataa ttggcgtttg gcaggtaagt
     1261 tgaatcaggg taatttttga tgaaattctg aaatgccacc attgcgtcat cctggcggga
     1321 tttatcctgc accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac
     1381 cggcgcgcca gcattcgcag taccagcatc agctgtcggc gtcgttgatg ccgccgcacc
     1441 gctttgatcg ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg
     1501 caacaggatc tgcttttgcc gctccacgac ctgattcagt tgatactgat tttcctgaat
     1561 ctgaccacgc agggaatcaa tatcggattg attatcagaa agttgttgct ggagttgggt
     1621 taaaagctgg ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt cttcgaccga
     1681 gcctgagccg acactactga ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc
     1741 aaccagtaac gacagactca atagttgatg tctgaagtta ctgctcatgc aattctctta
     1801 gtaaaccagt accgcacgac ggtttttgga gtatgccgct tcgtcatgac ccagtactgc
     1861 aggtttttct ttaccgtaag aaacgatgga gatctggtct gcagaaacgc ctttaccctg
     1921 caggtacatc ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt attccggagt
     1981 accacgttcg tccgcgtgac cttctacggt gactttgtaa gacgggttgc tacgcaggaa
     2041 gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg atatcgtact tgtccagatc
     2101 gaagtaaacg atgttgttct gctgcagctg ttgcatttgc agacgagcct gctcttcgga
     2161 agacatgttg ccgttgccgc cgttcgcatc cataccagtg ccggcaccca gcatgccttc
     2221 gctgccgtca ttgctggcgt tcttgttgga agaacatgcc gcaattgcca taacaggcag
     2281 agcaatcatc agccctttca gcactttgtt cagttgcatt tcaatgattc ctttactatt
     2341 caattaatta ttatcacaga tacggcgacc aggcagggaa tttgacctgt ccatcagttg
     2401 ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa attcagcacg gatcccatcc
     2461 cctgagaaga gctgtagatt accatagtgc cgttaggtgc cagacttggc gtttcatcca
     2521 ggaacgtgga cgacagaact tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct
     2581 gctgcccacc attggagctg accattacca taaatttacc gtcgctgctg acatccgcat
     2641 cctggttctg cgaaccttcc caggtaatac gttgtggcgc accgccgttg atattcactt
     2701 tataaacctg cggacgaccg gcctggtcag aagtaaatgc caggttctgg ctatccggga
     2761 accaggtcgg ttcggtattg ttactgcgac catcagtcac ctggcggatc tgaccagaag
     2821 ccaaatccat tacgtacagg ttcagactac cggttttcga caaggcgaat gccagtttgc
     2881 tgccgtctgg cgagaatgca ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta
     2941 cagcgccatt tgccagcgtc tgaataacca gcgcggaacg accgctttcg aaggtcacat
     3001 aagccagttt agaaccgtct ggtgaccacg ccggagacat cagcggctgc ggtgaacggt
     3061 gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg cagttcatac gggaactgac
     3121 cgccgttggt ctgaacaacg taggcaatac gggtacggaa cgcaccttta atgccggtca
     3181 gtttttcaaa cacttcatca ctggcggtat gaccagcata acgcagccac tgcttgttca
     3241 ctttgtacga gttctgagca agtacagtac ccggtgcgcc gccagtgtca acaagttgat
     3301 aagcaacatt gtaagaacca tccggattcg gagtgacctg accgacaact acagcgtcaa
     3361 tgcccagtgc ggaccatgca gctggttgta cttcctgcgc actacccggc tgctgtggca
     3421 gacgagcgcg atctaacgga ttaaatttac cgctgttacg caagtcagca gcaacgatgc
     3481 cgccaatatc ttcaggtgcc gcaccaggcc ccgcccactg gaaaggaaca acaccaatag
     3541 gacgaccgga atctacaccg ctgtcgatca caatgcggac ttcagcatgc agaactgatg
     3601 cccacagtat gagaaaacca aatgctactc gtaatgcctg cttcatcata tctcccttat
     3661 ctggaccgat ggcccacgat aatttagcag aatgttaaca aactaaaata cacaaaacta
     3721 ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcc gaacagtcaa catcgcgatt
     3781 acggtttgaa gtccaatggc gcgtttttga acacttcata tactgcctgg cttggtggtt
     3841 tcgggatctt cgcaagttta gctgctgcca acgcagcctg acaaagtgcg ggatcgccac
     3901 cttcaggttt gatatccagt aacataccat cgggtgccag ttttatgcgc agcgtacagg
     3961 ttttgcctgc ataggacgat gcgtcataga acttactttc gatagcagat ttaatctgcc
     4021 cggcatagtt attgatatcg gcccctgatg cgccattgtt tttagtatta ccactcccgg
     4081 caggcgaagc attgttccct ttcgcccctc cccccgtttt cggtgcattc ttaccagagc
     4141 ttagctcacc gaaaatatca tctgcctctg cggccgcttt tgctgcagcg gctttttcag
     4201 ctgcagcttt tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc gctgctttct
     4261 tatcagctgc tgccttttca gcagccgctt tttctgctgc ttttttgtcg gctgcagctt
     4321 tctctgctgc cgctttctta tcagctgcag ccttttcagc agccgctttc ttctcagctt
     4381 ctgctttggc tttttcagca gcttcagttg ccgctttctt tcttgcttca gctgcagctg
     4441 cttctgccgc ttccgctttc ttcttcagtg ccgcagcggc tgcctcggct tttttctgcg
     4501 cttcggctgc ggctttggcg gcttctgctt ctgctttttt ctttgcgtct gcagccgctt
     4561 tcttcgctgc ttcttccgca gctttagcat ctgcttcggc cttcgcttta gcatctgccg
     4621 ccgctttcgc tgccgcctct tcagcttgct tctgctttaa ctcggcctgt tttgcggctt
     4681 cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt tgcttcaggc
     4741 gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt tccttcatct
     4801 tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct
     4861 caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg ccgcctccgg
     4921 ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat aagatgacat
     4981 gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg gttgcctttg
     5041 acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag caagggaaac
     5101 gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc actatgtaac
     5161 aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc gatcagaaag
     5221 accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg ctctggtggt
     5281 aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc agacacttca
     5341 acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc gtctggcaga
     5401 tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag caacagcacc
     5461 agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg acgacctcgt
     5521 ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa cgcctggcgg
     5581 tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag tttgtttacg
     5641 cgctggttga ggcggttata agccattacc gccggaattg cggcgaacag accaattgca
     5701 gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc ttgttttacc
     5761 gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag accaatatac
     5821 gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt ttcaagttca
     5881 cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc cggcgcatgg
     5941 ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat ttgttccgaa
     6001 ccagtcagat tatcccgttt cccctggctc tcttgataga ggcgagagag ttcgattcca
     6061 gaccagaatt tatcttcaaa cgcttcggct tcgcgcgccg ctgcgttaag aatacgggtc
     6121 cgctggataa taatggccca agatgcgatt gaaaaaccaa tcaaaatcaa catgataagt
     6181 ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt cactgcttaa
     6241 actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg tcaacgcaaa
     6301 caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata cgttgcgtga
     6361 aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc atatcgtcga
     6421 gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca acgcgttcag
     6481 ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt gctctttcat
     6541 aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct tcatagtaga
     6601 cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat gcaacaaaag
     6661 ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa tgagggcaag
     6721 ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa agagaattct
     6781 gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa gcaatgggca
     6841 aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa tcacaccagc
     6901 acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg tcttcgcggc
     6961 aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca cgaaggaaag
     7021 cgcccgcagg gggcgcttgt ccattaccgc atacaaagtc gcgataatct tactcatcag
     7081 atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc ggtgattacc
     7141 ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat ttttagctcc
     7201 ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata cgaccgaaca
     7261 ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc accgcaaccc
     7321 aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc agacttgcgt
     7381 tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct gtcaggatga
     7441 tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc atacgtgcag
     7501 tcaggatggt cagcagcggc agaaccacac ccagcgccgg aatagcccac agaattggcg
     7561 tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta ttcagtgggt
     7621 tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata ccgtacatca
     7681 cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc tgagccgttg
     7741 cacgggtacg caggtgcagt tcgcccacgg tacgcatttg cagataggtt gcgccctgag
     7801 taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta agcaactgga
     7861 agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt acgccctgca
     7921 acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca atgaagatgc
     7981 cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag tcaaaaccga
     8041 ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag ccggagaacg
     8101 cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg atcagccaaa
     8161 cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc tcggtgtcgt
     8221 tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca gtgactgcaa
     8281 aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact tcataatcga
     8341 tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac tgctcaaagt
     8401 gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaatttgaac attaagaaca
     8461 attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag aagatgagat
     8521 cgcctgcggt cagtgacgag ttcgccacag ctgtcggcag cacttcaccg atagcccacg
     8581 gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac ggcagcggaa
     8641 taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg cggatgacac
     8701 tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc atgatacgga
     8761 acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt tgaatctgcg
     8821 cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg taaccgaggt
     8881 ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca gaacgcagtt
     8941 gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc tgcaccatca
     9001 gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg cccagtgcgt
     9061 aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg ccgaacagag
     9121 taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc agtttggttt
     9181 tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca gacagaacag
     9241 cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg aagtcacgac
     9301 ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc acataaccag
     9361 acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc tcggagaagc
     9421 tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg ttttgcatcc
     9481 agccgttcgc aaccagaatc cacagtgcgg acaggtttga accgagcgcc accagccagg
     9541 tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac agacctacaa
     9601 aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca ccgaagatat
     9661 cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc atggtcagac
     9721 cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac ttggtcatat
     9781 ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc agcaggaacg
     9841 ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag gcaaactgta
     9901 agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat gaagactccg
     9961 agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg tttgaacact
    10021 ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca aacatgttaa
    10081 taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca ataaacaggt
    10141 ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa attaccgcct
    10201 ttcagccaat ttgatcgaga acaatttatc tctttttgat gc
//
LOCUS       DAPPNJ010000158        10200 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000158 DAPPNJ010000000
VERSION     DAPPNJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.159"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RPM73_003401"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RPM73_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDX6521727.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RPM73_003402"
     CDS             complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RPM73_003402"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDX6521728.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RPM73_003403"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RPM73_003403"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDX6521729.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="RPM73_003404"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="RPM73_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDX6521730.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="RPM73_003405"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="RPM73_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDX6521731.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="RPM73_003406"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="RPM73_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDX6521732.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="RPM73_003407"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="RPM73_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDX6521733.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="RPM73_003408"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="RPM73_003408"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDX6521734.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="RPM73_003409"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="RPM73_003409"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDX6521735.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9874..>10200
                     /locus_tag="RPM73_003410"
     CDS             9874..>10200
                     /locus_tag="RPM73_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDX6521736.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQK"
BASE COUNT         2619 a         2699 c         2389 g         2493 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc caccattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc attgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgagagggtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tagctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagcagccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact tcctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
//
LOCUS       DAPPNJ010000159        10195 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000159 DAPPNJ010000000
VERSION     DAPPNJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.160"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(78..854)
                     /gene="yaaA"
                     /locus_tag="RPM73_003411"
     CDS             complement(78..854)
                     /gene="yaaA"
                     /locus_tag="RPM73_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDX6521737.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNTEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(924..2354)
                     /locus_tag="RPM73_003412"
     CDS             complement(924..2354)
                     /locus_tag="RPM73_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDX6521738.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2633..3586
                     /gene="tal"
                     /locus_tag="RPM73_003413"
     CDS             2633..3586
                     /gene="tal"
                     /locus_tag="RPM73_003413"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDX6521739.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            3701..4288
                     /gene="mog"
                     /locus_tag="RPM73_003414"
     CDS             3701..4288
                     /gene="mog"
                     /locus_tag="RPM73_003414"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDX6521740.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4323..4889)
                     /gene="satP"
                     /locus_tag="RPM73_003415"
     CDS             complement(4323..4889)
                     /gene="satP"
                     /locus_tag="RPM73_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDX6521741.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5038..5751)
                     /gene="msyB"
                     /locus_tag="RPM73_003416"
     CDS             complement(5038..5751)
                     /gene="msyB"
                     /locus_tag="RPM73_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDX6521742.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5777..6181)
                     /locus_tag="RPM73_003417"
     CDS             complement(5777..6181)
                     /locus_tag="RPM73_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDX6521743.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6558..8474
                     /gene="dnaK"
                     /locus_tag="RPM73_003418"
     CDS             6558..8474
                     /gene="dnaK"
                     /locus_tag="RPM73_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDX6521744.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8563..9693
                     /gene="dnaJ"
                     /locus_tag="RPM73_003419"
     CDS             8563..9693
                     /gene="dnaJ"
                     /locus_tag="RPM73_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDX6521745.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9797..9949)
                     /gene="mokC"
                     /locus_tag="RPM73_003420"
     CDS             complement(9797..9949)
                     /gene="mokC"
                     /locus_tag="RPM73_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDX6521746.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2667 a         2550 c         2644 g         2334 t
ORIGIN      
        1 ttctgcaata tattgaatct gcatgctttt gtaggccgga taaggcgttc acgccgcatc
       61 cggcattggc tacaaactta acgctgctcg tagcgtttaa acaccagttc accattgctg
      121 gaggaagctt catcaaagaa gtaaccttcg gtattaaaac cagtcagttg ctctggtttg
      181 gtcagccgat tttcaataat gaaacgactc atcagaccgc gtgctttctt agcgtagaag
      241 ctgatgatct taaatttgcc gttcttctca tcgaggaaca ccggcttgat aatctcggca
      301 ttcaatttct tcggcttcac cgatttaaaa tactcatctg acgccagatt aatcaccaca
      361 ttatcgcctt gtgctgcgag cgcctcgttc agcttgttgg tgatgatatc tccccagaat
      421 tgatacagat ctttccctcg ggcattctca agacggatcc ccatttccag acgataaggc
      481 tgcattaaat cgagcgggcg cagtacgcca tacaagccgg aaagcattcg caaatgctgt
      541 tgggcaaaat cgaaatcgtc ttcgctgaag gtttcggcct gcaagccggt gtagacatca
      601 cctttaaacg ccagaatcgc ctggcgggca ttctccggcg tgaaatctgg ctgccagtca
      661 tgaaagcgag cggcgttgat acccgccagt ttgtcgctga tgcgcatcag cgtgctaatc
      721 tgcggaggcg tcagtttccg cgcttcatgg atcaactgct gggaattgtc taacagctcc
      781 ggcagcgtat agcgcatggt ggtcaacggg ctttggtaat caagcgtttt cgcaggtgaa
      841 ataagaatca gcatatccag tccttgcagg aaatttatgc cgactttagc aaaaaaagag
      901 aatgagttga tcgatagttg tgattactcc tgcgaaacat catcccacgc gtccggagaa
      961 agctggcgac cgatatccgg ataacgcaac ggatcaaaca ccgggcgcac gccgagttta
     1021 cgctggcgta gataatcact ggcaatggta tgaaccacag gcgagagcag taaaatggcg
     1081 gtcaaattgg taatagccat gcaggccatt atgatatctg ccagttgcca catcagcgga
     1141 aggcttagca aggtgccgcc gatgaccgtt gcgaaggtgc agatccgcaa acaccagatc
     1201 gctttagggt tgttcaggcg taaaaagaag agattgtttt cggcataaat gtagttggca
     1261 acgatggagc tgaaggcaaa cagaataacc acgagggtaa caaactcagc accccaggaa
     1321 cccattagca cccgcatcgc cttctggata agctgaatac cttccagcgg catgtaggtt
     1381 gtgtcgttac ccgccagtaa tatcagcatg gcgcttgccg tacagatgac cagggtgtcg
     1441 ataaaaatgc caatcatctg gacaattcct tgcgctgccg gatgcggagg ccaggacgcc
     1501 gctgccgctg ccgcgtttgg cgtcgaaccc attcccgcct cattggaaaa catactgcgc
     1561 tgaaaaccgt tagtaatcgc ctggcttaag gtatatcccg ccgcgccgcc tgccgcttcc
     1621 tgccagccaa aagcactctc aaaaatagac caaatgacgt ggggaagttg cccgatattc
     1681 attacgcaaa tcaccaggct ggtcagtacc cagattatcg ccatcaacgg gacaaacccc
     1741 tgcatgagcc gggcgacgcc atgaagaccg cgagtgattg ccagcagagc aaagacagcg
     1801 agaataatgc ctgtcaccag cggaggaaaa tcaaaagaaa aacttagggc gcgggcaacg
     1861 gcgttcgcct gaactccgct gaaaattatg ccataggcga tgagcaaaaa gacggcgaac
     1921 agaacgccca tccaacgcat ccccagcccg cgcgccatat accatgccgg tccgccacga
     1981 aactgtccat tgacgtcacg ttctttataa agttgtgcca gagaacattc ggcaaacgag
     2041 gtcgccatgc cgataaacgc ggcaacccac atccaaaaga cggctccagg tccaccggcg
     2101 gtaattgcca gcgcaacgcc ggccaggttg ccgctaccta cgcgcgccgc aagactggta
     2161 cacaatgact gaaatgaggt taaaccgcct ggctgtggat gaatgctatt tttaagactt
     2221 ttgccaaact ggcggatgta gcgaaactgc acaaatccgg tgcgaaaagt gaaccaacaa
     2281 cctgcgccga agagcaggta aatcattacc gatccccaaa ggacgctgtt aataaaggag
     2341 aaaaaatctg gcatgcatat ccctcttatt gccggtcgcg atgactttcc tgtgtaaacg
     2401 ttaccaattg tttaagaagt atatacgcta cgaggtactt gataacttct gcgtagcata
     2461 catgaggttt tgtataaaaa tggcgggcga tatcaacgca gtgtcagaaa tccgaaacag
     2521 tctcgcctgg cgataaccgt cttgtcggcg gttgcgctga cgttgcgtcg tgatatcatc
     2581 agggcagacc ggttacatcc ccctaacaag ctgtttaaag agaaatacta tcatgacgga
     2641 caaattgacc tcccttcgtc agtacaccac cgtagtggcc gacactgggg acatcgcggc
     2701 aatgaagctg tatcaaccgc aggatgccac aaccaaccct tctctcattc ttaacgcagc
     2761 gcagattccg gaataccgta agttgattga tgatgctgtc gcctgggcga aacagcagag
     2821 caacgatcgc gcgcagcaga tcgtggacgc gaccgacaaa ctggcagtaa atattggtct
     2881 ggaaatcctg aaactggttc cgggccgtat ctcaactgaa gttgatgcgc gtctttccta
     2941 tgacaccgaa gcgtcaattg cgaaagcaaa acgcctgatc aaactctaca acgatgctgg
     3001 tattagcaac gatcgtattc tgatcaaact ggcttctacc tggcagggta ttcgtgctgc
     3061 agaacagctg gaaaaagaag gtatcaactg taacctgacc ctgctgttct ccttcgctca
     3121 ggctcgtgct tgtgcggaag cgggcgtgtt cctgatctcg ccgtttgttg gccgtattct
     3181 tgactggtac aaagcgaata ccgataagaa agagtacgct ccggcagaag atccgggcgt
     3241 ggtttctgta tctgaaatct accagtacta caaagagcac ggttatgaaa ccgtggttat
     3301 gggcgcaagc ttccgtaaca tcggcgaaat tctggaactg gcaggctgcg accgtctgac
     3361 catcgcaccg gcactgctga aagagctggc tgagagcgaa ggggctattg aacgtaaact
     3421 gtcttacacc ggcgaagtga aagcgcgtcc ggcgcgtatc actgagtccg agttcctgtg
     3481 gcagcacaac caggatccaa tggcagtaga taaactggcg gaaggtatcc gtaagtttgc
     3541 tgttgaccag gaaaaactgg aaaaaatgat cggcgatctg ctgtaatcat tcttagcgtg
     3601 accgggaagt cggtcacgct acctcttctg aagcctgtct gtcactccct tcgcagtgta
     3661 tcattctgtt taacgagact gtttaaacgg aaaaatcttg atgaatactt tacgtattgg
     3721 cttagtttcc atctctgatc gcgcatccag cggcgtttat caggataaag gtatccctgc
     3781 actggaagaa tggctgacat cggcgctaac cacgccgttt gaactggaaa cccgcttaat
     3841 tcccgatgag caggcgatca tcgagcaaac gttgtgtgag ctggtggatg aaatgagttg
     3901 ccatctggtg ctcaccacgg gcggaactgg cccggcgcgt cgtgacgtaa cgcccgatgc
     3961 gacgctggca gtagcggacc gcgagatgcc tggctttggt gaacagatgc gccagatcag
     4021 cctgcatttt gtaccaactg cgatcctttc gcgtcaggtg ggcgtgattc gcaaacaggc
     4081 gctgatcctt aacttacccg gccagccgaa gtctattaaa gagacgctgg aaggtgtgaa
     4141 ggacgctgag ggtaacgttg tggtacacgg tatttttgcc agcgtaccgt actgcattca
     4201 gttgctggaa gggccatacg ttgaaacggc accagaagtg gttgctgcat tcagaccgaa
     4261 gagtgcaaga cgcgacgtta gcgaataaaa aaatcccccc gagcgggggg atctcaaaac
     4321 aattagtggg attcaccaat cggcagaacg gtgcgaccaa actgctcgtt cagtacttca
     4381 cccatcgcca gatagattgc gctggcaccg cagatcagac caatccagcc ggcaaagtgg
     4441 atgattgcgg cgttaccggc aatgttaccg atcgccagca gggcaaacag cacggtcagg
     4501 ctaaagaaaa cgaattgcag aacgcgtgcg cctttcagcg tgccgaagaa cataaacagc
     4561 gtaaatacgc cccacagacc caggtagaca ccaaggaact gtgcatttgg cgcatcggtc
     4621 agacccagtt tcggcatcag cagaatcgca accagcgtca gccagaaaga accgtaagag
     4681 gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact ccagcagacc agcaaaaatt
     4741 tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa taccgtccag agcgaaataa
     4801 cccacgttgt gcaggttaag cagaatggtg gtcatgccga agcccatcag gcccagcggt
     4861 gccggattag ccaacttagt gttgcccata attcctcaaa aatcatcatc gaatgaatgg
     4921 tgaaataatt tccctgaata actgtagtgt tttcagggcg cggcataata atcagccagt
     4981 ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt ttccccagtt tacggtatca
     5041 gacctgagtg gcgctaacca tccggcgcag gcaggcgatt tgcagtacgg ctggaatcgt
     5101 cacgcgatag gcgctgccgc tgaccgcttt aaccccattt agtgccgcac ctacagggcc
     5161 tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg ctcattgctg cgtgggtgcg
     5221 taaaatgcgg gtcagttggc tggaaagcaa atgcgacacg ccttttgcca ataatttgtc
     5281 tttcatcagc agcggcagca gctcttccag ctcattcacc ctcgcatcga ccgcgtgcag
     5341 aaactcctgt ttatgttcct cgtccatatt cttccaggtg ttacgcagaa attgttccag
     5401 taactgttgc tcaatctcaa acgtagacat ctctttgtcg gctttcagct tcaatcgttt
     5461 tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt ccgcgcagtt tgttggcgat
     5521 actatcgcca ccaaaatgct gtaattctcc agcaatcagc tgccagttgc ggcgatgttg
     5581 ctcgggatgc ccttccatag atttaaacag ttcgttgcgc atcagtacgc tggagaggcg
     5641 agttttgcct ttttcattat gggtgagcaa tcgggcgaaa tttgccaact gttcctcact
     5701 gcaatgctgg agaaaatcca gatctgaatc attcaggtaa ttaacattca ttttttgtgg
     5761 cttctatatt ctggcgttag tcgtcgccga taattttcag cgtggccata tccgatgagt
     5821 tcaccgtatg accggaaaag gtgatttttg agacgcagcg tttattgtcg ttatcgctgt
     5881 taatgttgat ccagtcagtg gtttgccctt cttttatttc tgaaggaata ttcaggctct
     5941 gactggcgct acgggcggct ttgaaataaa ccgatgcacc gcttaactgt aaatcgccat
     6001 ggtcggcaga gagttgtatg cgtttaacaa ttcggcaaac aggaagtttc agcgccagat
     6061 cgttggtttc gttacgtggc atcgcaatga cgccgaggat tttatggtcg tttgcctgcg
     6121 ccgtgcagca cagcatcaga ctaatcgcca ggctggcgga aatcgtaaaa acggatttca
     6181 taaggattct cttagtgggg agaggtaggg ggatggatac ccactagttt actgctgata
     6241 aagagaagat tcaggtgcgt aatgttttct ttttattaca attttttgat gaatgtcttg
     6301 gctgcgattc attctttata tgaataaaat tgctgtcagt ttcacgtctt gtcctgctat
     6361 atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca tctccccctt gatgacgtgg
     6421 gttacgaccc catttagtag tcaaccgcag tgagtgagtc tgcaaaaaaa tgaaattggg
     6481 cagttgaaac cagacgtttc gcccctatta cagactcaca accacatgat gaccgaatat
     6541 atagtggaga cgtttagatg ggtaaaataa ttggtatcga cctgggtact accaactctt
     6601 gtgtagcgat tatggatggc accactcctc gcgtgctgga gaacgccgaa ggcgatcgca
     6661 ccacgccttc tatcattgcc tatacccagg atggtgaaac tctggttggt cagccggcta
     6721 aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc gattaaacgc ctgattggcc
     6781 gccgcttcca ggacgaagaa gtacagcgtg atgtttccat catgccgttc aaaattattg
     6841 ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca gaaaatggca ccgccgcaga
     6901 tttctgctga agtgctgaaa aaaatgaaga aaaccgctga agattacttg ggtgaaccgg
     6961 taactgaagc tgttatcacc gtaccggcat actttaacga tgctcagcgt caggcaacca
     7021 aagacgcagg ccgtatcgct ggtctggaag taaaacgtat catcaacgaa ccgaccgcag
     7081 ctgcgctggc ttacggtctg gacaaaggta ctggcaaccg tactatcgcg gtttatgacc
     7141 tgggtggtgg tactttcgat atttctatta tcgaaatcga cgaagttgac ggcgaaaaaa
     7201 ccttcgaagt tctggctacc aacggtgata cccacctggg tggtgaagac ttcgacagcc
     7261 gtctgatcaa ctacctggtt gaagaattca agaaagatca gggcattgac ctgcgcaacg
     7321 atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa agcgaaaatc gaactgtctt
     7381 ccgctcagca gaccgacgtt aacctgccgt acatcactgc agatgcgacc ggtccgaaac
     7441 acatgaacat caaagtgact cgtgcgaaac tggaaagcct ggttgaagat ctggtaaacc
     7501 gttccattga gccgctgaaa gttgcactgc aggacgctgg cctgtccgta tctgatatcg
     7561 acgacgttat cctcgttggt ggtcagactc gtatgccaat ggttcagaag aaagttgctg
     7621 agttctttgg taaagagccg cgtaaagacg ttaacccgga cgaagctgta gcaatcggtg
     7681 ctgctgttca gggtggtgtt ctgactggtg acgtgaaaga cgtactgctg ctggacgtta
     7741 ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat gacgacgctg atcgcgaaaa
     7801 acaccactat cccgaccaag cacagccagg tgttctctac cgctgaagac aaccagtctg
     7861 cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc ggctgataac aaatctctgg
     7921 gtcagttcaa cctggatggt atcaacccgg caccgcgcgg catgccgcag atcgaagtta
     7981 ccttcgatat cgatgctgac ggtatcctgc acgtttccgc gaaagataaa aacagcggta
     8041 aagagcagaa gatcaccatc aaggcttctt ctggtctgaa cgaagatgaa atccagaaaa
     8101 tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa gtttgaagag ctggtacaga
     8161 ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa gcaggttgaa gaagcaggcg
     8221 acaaactgcc ggctgacgac aaaactgcta tcgagtctgc actgactgca ctggaaactg
     8281 ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat gcaggagctg gcacaggttt
     8341 cccagaaact gatggaaatc gcccagcagc aacatgccca gcagcagact gccggtgctg
     8401 atgcttctgc aaacaacgcg aaagatgacg atgttgtcga cgctgaattt gaagaagtca
     8461 aagacaaaaa ataatcgccc tataaacggg taattatact gacacgggcg aaggggaatt
     8521 tcctctccgc ccgtgcattc atctaggggc aatttaaaaa agatggctaa gcaagattat
     8581 tacgagattt taggcgtttc caaaacagcg gaagagcgtg aaatcaaaaa ggcctacaaa
     8641 cgcctggcca tgaaatacca cccggaccgt aaccagggtg acaaagaggc cgaggcgaaa
     8701 tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact cgcaaaaacg tgcggcatac
     8761 gatcagtatg gtcatgctgc gtttgagcaa ggcggcatgg gcggcggcgg ttttggcggc
     8821 ggcgcagact tcagcgatat ttttggtgac gttttcggcg atatttttgg cggcggacgt
     8881 ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata acatggagct caccctcgaa
     8941 gaagctgtac gtggcgtgac caaagagatc cgcattccga ctctggaaga gtgtgacgtt
     9001 tgccacggta gcggtgcaaa accaggtaca cagccgcaga cctgtccgac ctgtcatggt
     9061 tctggtcagg tgcagatgcg tcagggattc ttcgctgtac agcagacctg tccacactgt
     9121 cagggccgcg gtacgctgat caaagatccg tgcaacaaat gtcatggtca tggtcgtgtt
     9181 gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg tggacactgg agaccgcatc
     9241 cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac cggcaggcga tctgtacgtt
     9301 caggttcagg ttaaacagca cccgattttc gagcgtgaag gcaacaacct ctattgcgaa
     9361 gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa tcgaagtacc gacccttgat
     9421 ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg gtaagctttt ccgtatgcgc
     9481 ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg atttgctgtg ccgcgttgtc
     9541 gtcgaaacac cggtaggcct gaacgagaag cagaaacagc tgctgcaaga gctgcaagaa
     9601 agcttcggtg gcccaaccgg tgagcacaac agcccgcgct caaagagctt ctttgatggt
     9661 gtgaagaagt tttttgacga cctgacccgc taacctcccc aaaagcctgc ccgtgggcag
     9721 gcttgggtaa aattagggtg cgttgaagat atgcgagcac ctgaaaagtg gcggggatca
     9781 ctccccgccg ttgcacttac tcggattcgt aagccgtgaa aacagcaacc tccgtctggc
     9841 cagttcggat gtgaacctca cagaggtctt ttctcgttac cagcgccgcc actacggcgg
     9901 tgatacagat gacgatcagg gcgacaatca tcgccttatg ctgcttcatt gctctcttct
     9961 ccttgacctt acggtcagta agaggcactc tacatgtgtt cagcatatag ggggcctcgg
    10021 gttgatagta aaatatcact cggggctttt ctctatctgc cgttcagcta atgcctgaga
    10081 cagatagcct caagcacccg ccgctattat atcgctctct ttaacccatt ctgttttatc
    10141 gattctaatc ctgaagacgc ctcgcatttt tgtggcgtaa ttttttaatg attta
//
LOCUS       DAPPNJ010000160        10105 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000160 DAPPNJ010000000
VERSION     DAPPNJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.161"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            485..1462
                     /gene="csiD"
                     /locus_tag="RPM73_003421"
     CDS             485..1462
                     /gene="csiD"
                     /locus_tag="RPM73_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDX6521747.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1482..2750
                     /gene="lhgO"
                     /locus_tag="RPM73_003422"
     CDS             1482..2750
                     /gene="lhgO"
                     /locus_tag="RPM73_003422"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDX6521748.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2773..4221
                     /gene="gabD"
                     /locus_tag="RPM73_003423"
     CDS             2773..4221
                     /gene="gabD"
                     /locus_tag="RPM73_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDX6521749.1"
                     /translation="MKLNASNLLRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4235..5515
                     /gene="gabT"
                     /locus_tag="RPM73_003424"
     CDS             4235..5515
                     /gene="gabT"
                     /locus_tag="RPM73_003424"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDX6521750.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5826..7226
                     /gene="gabP"
                     /locus_tag="RPM73_003425"
     CDS             5826..7226
                     /gene="gabP"
                     /locus_tag="RPM73_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDX6521751.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7247..7909
                     /gene="csiR"
                     /locus_tag="RPM73_003426"
     CDS             7247..7909
                     /gene="csiR"
                     /locus_tag="RPM73_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDX6521752.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7910..8359)
                     /gene="kbp"
                     /locus_tag="RPM73_003427"
     CDS             complement(7910..8359)
                     /gene="kbp"
                     /locus_tag="RPM73_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDX6521753.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8443..8601)
                     /locus_tag="RPM73_003428"
     CDS             complement(8443..8601)
                     /locus_tag="RPM73_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDX6521754.1"
                     /translation="MGFWRIVITIILPPLGVLLSKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8784..9083
                     /locus_tag="RPM73_003429"
     CDS             8784..9083
                     /locus_tag="RPM73_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6521755.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9093..9617
                     /gene="ygaP"
                     /locus_tag="RPM73_003430"
     CDS             9093..9617
                     /gene="ygaP"
                     /locus_tag="RPM73_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDX6521756.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9664..10068)
                     /gene="stpA"
                     /locus_tag="RPM73_003431"
     CDS             complement(9664..10068)
                     /gene="stpA"
                     /locus_tag="RPM73_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDX6521757.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2312 a         2590 c         2765 g         2438 t
ORIGIN      
        1 gatgcgttca gcaagcttca gggcttcact gcgaaattca ggcgaatgct gtttacgggg
       61 ttttttactg gttgatactg tttttgtcat gtgagtcacc tctgactgag agtttactca
      121 cttagccgcg tgtccactat tgctgggtaa gatcagttat atctgccccg ataaaacggg
      181 gcagataata tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt
      241 atcgaccaga taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc
      301 atttcctgtc tcactttgcc ttaataccct acgttaaatg ttactaattt gttgcttttg
      361 atcacaataa gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt
      421 tagattatgg ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct
      481 tctgatgaat gcactgaccg ccgtacacaa taacgctgtc gattcaggcc aggactatag
      541 cggattcact ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga
      601 acagacgacc aaacagtttc tcgaacaggt tgccgaatgg cccgtgcagg cgctggagta
      661 caaatcgttt ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct
      721 gcaaccattg ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc
      781 ggtgggtatc gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc
      841 gcatctgatt ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt
      901 ggtgaaaaat gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact
      961 gcacaacgac ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga
     1021 tgagcaaaac atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct
     1081 ggaccactat ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag
     1141 caaaaacgtc agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc
     1201 ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt
     1261 gagcgagctt tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt
     1321 tggcaaattc ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc
     1381 gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca
     1441 ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta
     1501 ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg
     1561 cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca
     1621 gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc
     1681 tggcgggaaa ccgcgccacc aaagcctttt gcgatcaaaa cgacattcgc tatgacaact
     1741 gcggcaagat gctggtcgcc acgtccgatc tcgaaatgga acggatgcgc gcgttgtggg
     1801 aacgcacggc ggcgaacggt atcgagcgcg agtggttaaa cgccgaggaa ctgcgcgagc
     1861 gcgaaccgaa tatcaccggg ctcggcggca tttttgtgcc gtccagcggc attgtcagct
     1921 accgcgaagt aacggcggcg atggcaaaaa ttttccaggc cagaggcggc gagattatct
     1981 ataacgccga agtcagcggc cttagtgagc ataaaagcgg cgtggtgata cgtacccgtc
     2041 agggcggcga ctatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc
     2101 tggtgaaaat gctcggcctc gaaccgggct ttatcatctg cccgttccgc ggcgagtatt
     2161 tccgcctggc gccggagcat aaccagattg ttaaccatct gatttacccc attcccgacc
     2221 cggcaatgcc gtttttgggc gtgcatctca cccgaatgat cgatggcagc gtcaccgtcg
     2281 ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta
     2341 gcgacacgct ggagattttg ggctcgtcgg ggattcgccg ggtgctgcaa aaccatctac
     2401 gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cgcctggtgc
     2461 aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg
     2521 cgcaggcagt atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac
     2581 gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg
     2641 cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc
     2701 tgcgtgcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa
     2761 caggacgtaa caatgaaact taacgccagt aacttactcc gccagcaggc attgattaac
     2821 ggggaatggc tggacgccaa caatggcgag gtcatcgacg tcaccaatcc ggcgaacggc
     2881 gacaagctgg gtagcgtacc caaaatgggc gctgatgaaa cccgcgcggc tatcgacgcc
     2941 gccaaccgcg ccctgcccgc ctggcgcgcg ctcaccgcca aagaacgcgc caccattctg
     3001 cgcaactggt tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgaccctc
     3061 gaacagggta aaccgctggc tgaagcgaaa ggcgaaatca gctacgccgc ctcctttatt
     3121 gagtggtttg ctgaagaagg caaacgtatt tatggcgaca ccattcccgg tcatcaggcc
     3181 gataaacgcc tgattgttat caagcagccg attggcgtca ctgctgctat cacgccgtgg
     3241 aactttccgg cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggttgcacc
     3301 atggtgctga agcccgccag tcagacgccg ttctctgcgc tggcgctggc agagctggcg
     3361 atccgcgcgg gcattccggc tggcgtgttt aacgtggtca ccggttcggc gggcgcggtg
     3421 ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ctcgaccgaa
     3481 attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg
     3541 ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc
     3601 gcgctggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac
     3661 gtgcaggacg gcgtgtatga ccgctttgcc gaaaaactac agcaggcggt gagcaaactg
     3721 cacatcggcg acgggctgga taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg
     3781 gtagcaaaag tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc
     3841 ggcggtaaag cggatgaacg cggcggcaac ttcttccagc cgaccattct ggtggacgtg
     3901 ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgc
     3961 tttaaagatg aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc
     4021 tatttctacg cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc
     4081 atcgtcggca tcaataccgg cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa
     4141 gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa
     4201 tatatgtgca tcggtcttta actggagaac gcgaatgagc agcaataaag agttaatgca
     4261 gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg
     4321 cgcggaaaac tgccgggtgt gggacgttga aggccgtgag tatcttgatt tcgcgggcgg
     4381 gattgcggtg ctcaataccg ggcacctgca tccgaaggtg gtggccgcgg tggaagcgca
     4441 gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct
     4501 gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac
     4561 gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag
     4621 cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg cattacacgc tggcgctgac
     4681 cggcaaggtg aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc
     4741 gctttatcct tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg
     4801 gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc atcgtgattg agccggttca
     4861 gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgcgctgtg
     4921 tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg
     4981 cacgctgttt gcgatggagc agatgggcgt tgcgccggat ctcactacct ttgcgaaatc
     5041 gatcgcgggc ggcttcccgc tggcgggcgt caccgggcgc gcggaagtga tggatgccgt
     5101 cgctccaggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggcggcgct
     5161 ggaagtgttg aaggtgtttg agcaggaaaa tctgctgcaa aaagccaacg atctggggca
     5221 gaagctaaaa gacggattgt tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg
     5281 cgggctggga gcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga
     5341 cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc
     5401 ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg ctcaccattg aagacgctca
     5461 gatccgtcag ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagctatg
     5521 tccgtttgta agagttaaac gttgctaagg gaatgataac gatgcagtaa aaaataataa
     5581 cgatgagtcg atcgacaaag ttaaaaacga ctgcggactg tcccctcgct cctctgggga
     5641 gagggttagg gtgaggggta atcacgcggc tacaccctca ccccggccct ctccctaagg
     5701 gagagggaga aaactctccc gctttcgttt ttaagatttt gttgaccgtc tcaattaaac
     5761 gcgtgacccg acgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat
     5821 tcaggatggg gcaatcatcg caaccacatg agttaggcgg cgggctgaag tcacgccacg
     5881 tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg
     5941 ccatcgccga agcgggcccg gcggtattac tggcctatct gttcgccgga ttactggtgg
     6001 ttatgattat gcgaatgctg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt
     6061 ccacctatgc cgataaagcc attggccgct gggcgggtta taccatcggc tggctgtact
     6121 ggtggttttg ggtactggtt atcccgctgg aagccaacat cgccgctatg atcctgcact
     6181 cgtgggttcc aggcattccc atctggttat tttccctcgt cattaccctc gccttaactg
     6241 gcagtaattt attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca
     6301 aagtcatcgc tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc
     6361 cttatgccga agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg
     6421 gcttcggtgc ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa
     6481 ttgtcaccat tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgctacca
     6541 actcggttat ctggcgtatt tctatcttct atttgtgctc tatttttgtc gtagtggcgt
     6601 taataccgtg gaatatgccg gggctgaaag ccgttggttc ttatcgctcg gttctggaat
     6661 tgctcaatat tccccatgcg aaattaatca tggactgcgt gatattactt tccgtaacca
     6721 gctgtctgaa ctcggcgctg tataccgcgt caaggatgct ctactcctta agccgtcgcg
     6781 gtgatgcgcc cgcggtaatg ggcaaaatca accgcagtaa aaccccgtat gtggcggtgt
     6841 tactctccac cggagcggca tttttaacgg tggtggtgaa ctattacgca cctgcgaaag
     6901 tgtttaaatt cctgatagac agctccggtg ctatcgccct gctggtttat ttagtcatcg
     6961 ccgtttcaca gttgcggatg cgtaaaattc tgcgagcaga aggaagcgaa attcgcttgc
     7021 gcatgtggct ttacccgtgg ctcacctggc tggtaatagg ctttattacc tttgtgttgg
     7081 tggtgatgtt attccgcccg gcgcaacagt tagaagtgat ctctaccggc ttattagcga
     7141 tagggattat ctgtaccgtg ccgattatgg cgcgctggaa aaagctggta ttgtggcaaa
     7201 aaacacccgt tcataatacg cgctgataat gatcaggagt cacaccatga ccattacgtc
     7261 tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga
     7321 aaaattacga atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttacggga
     7381 agctctttcg caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg
     7441 ggtggcgtct atgtcagagc aggagctgct cgatattttc gacgcccgcg ccaatatgga
     7501 agcgatgctg gtcagtctgg cgattgcccg cggtggcgat gaatgggagg cagacgttct
     7561 cgcaaaagcg catctgctga gtaagctgga ggcctgtgac gccagcgaga aaatgcttga
     7621 tgagtgggat ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct gtggttctca
     7681 ctatttgctg caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc gatttatctg
     7741 gctgcggcga acggtgcttt cggtagaaat gctggaggat aaacacgatc agcaccagac
     7801 cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca
     7861 tttactgacg ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg
     7921 gaatacgcaa cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg
     7981 cttcgaagat tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca
     8041 gagtgtcgcc agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca
     8101 cctgatcatc aacactggca ataccggaaa tattccccac cgcaacaagg attttctcct
     8161 tcgcctcctg actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt
     8221 tcactttatc ggcatcaggt ataccggttt tgctcagatg ctcctgcacc ttcttcgcct
     8281 gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca
     8341 caaaattgaa cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc
     8401 agaggaacgc ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac
     8461 ccagaacgcg tgaatcaaac cggggatata cccaagcagt gtcagcagaa tattaataat
     8521 gaacgcccaa ccgaaccctt tactgagcag cacgccgaga ggcggcagaa tgatggtgat
     8581 gacgattctc cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag
     8641 agattttatc tcttaagcgt agtaatttta gcggagcctg ccagtttcag cttacgcttt
     8701 tactctcatt tacgctgctg atgctggtat atgttgcatt tttagtttag agtttactta
     8761 atttagaaaa tacttaaata aatatgactg aactcgcgca attacaggcc agtgccgaac
     8821 aggcagcggc cttattgaaa gcaatgagcc atcctaaacg attgctgatt ctgtgcatgc
     8881 ttagcggttc ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagtgcct
     8941 ctgcgacatc acagcatctc gctcgtatgc gggacgaagg gcttatcgac agccaacggg
     9001 atgcccaacg cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc
     9061 tgaaaaatgt ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg
     9121 atgcacaaga attaatcgca cgcggcgcaa agttaatcga tattcgtgat gctgatgaat
     9181 atcttcgtga acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag
     9241 gtcttccacc taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca
     9301 ccagtaataa cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg
     9361 aagatgggat tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac
     9421 ccttgccgtt aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg
     9481 tactgggtta taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt
     9541 tactgtttgc aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt
     9601 ggaaccaacg agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag
     9661 taattagatc aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt
     9721 cggtgtacgg ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg tgaatttata
     9781 tttcgccgga cgcggctggc gttttttacc agcgcgtggt gcagcagcag agctattacc
     9841 caataactct tccgggttaa ttccgtcagc cttcatcagc tccagccagg tgctaatttt
     9901 ttcctgacgc tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt
     9961 gacaaccctg aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat
    10021 cgcacggagg gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac
    10081 cttttaacgc caaaacaaaa aagta
//
LOCUS       DAPPNJ010000161        10090 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000161 DAPPNJ010000000
VERSION     DAPPNJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.162"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..421)
                     /gene="folK"
                     /locus_tag="RPM73_003432"
     CDS             complement(<1..421)
                     /gene="folK"
                     /locus_tag="RPM73_003432"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDX6521758.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPD"
     gene            complement(418..1836)
                     /gene="pcnB"
                     /locus_tag="RPM73_003433"
     CDS             complement(418..1836)
                     /gene="pcnB"
                     /locus_tag="RPM73_003433"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDX6521759.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1875..2801)
                     /gene="gluQRS"
                     /locus_tag="RPM73_003434"
     CDS             complement(1875..2801)
                     /gene="gluQRS"
                     /locus_tag="RPM73_003434"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDX6521760.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVNNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2838..3293)
                     /gene="dksA"
                     /locus_tag="RPM73_003435"
     CDS             complement(2838..3293)
                     /gene="dksA"
                     /locus_tag="RPM73_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDX6521761.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3471..4175)
                     /gene="sfsA"
                     /locus_tag="RPM73_003436"
     CDS             complement(3471..4175)
                     /gene="sfsA"
                     /locus_tag="RPM73_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDX6521762.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4190..4720)
                     /gene="thpR"
                     /locus_tag="RPM73_003437"
     CDS             complement(4190..4720)
                     /gene="thpR"
                     /locus_tag="RPM73_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDX6521763.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4794..7223
                     /gene="hrpB"
                     /locus_tag="RPM73_003438"
     CDS             4794..7223
                     /gene="hrpB"
                     /locus_tag="RPM73_003438"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDX6521764.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7419..9953
                     /gene="mrcB"
                     /locus_tag="RPM73_003439"
     CDS             7419..9953
                     /gene="mrcB"
                     /locus_tag="RPM73_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDX6521765.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
BASE COUNT         2312 a         2608 c         2879 g         2291 t
ORIGIN      
        1 catcaggaaa cgccagctcc ggtgcaatct caaacagcgg ccacagcata aatccacgat
       61 tcttcatatc gtagtgcgga acggtcaggc gttcggtatt tatcacttca ttaccaaaca
      121 gcatgatatc gagatccagc gtgcgtggtc cccagcgttc agctttacga acacgaccct
      181 gttgcagttc tatgcgctgt gtatggttga gtaactcttc cggggcgagc gaagtttcaa
      241 gcgccacggc ggcgttgagg taatcgggtt gatcttgcgg ccccagcggt ggggtgcggt
      301 aaaacgaaga aacggcaaga atgtggcttt cagggatatc gcctaatgct ttcagggcag
      361 cattgacctg ctccagcgga gaggccagat tgctgcctat ggcaatatac gccactgtca
      421 tgcggtaccc tcacgacgcg gtgcgcgttt gcgtggacga cgagtacgac gacgcggtga
      481 cggttcttca tccagctcgt taagcatccc tttttgatct ggtggtgcgg aaacctggaa
      541 ctcaccccac catttcacca gacgctgcag ttcagcatta cgttcaactt cagctcgcaa
      601 ggccaacagg tcataagccg cacggaactt aggatgctcc agcagtttcc atgcgcgttt
      661 accctgacga cgggacatac gcaactgcaa ctgccagata tcgcgggtta acgtcgtcag
      721 acgtttcggg attgccagtg aacggcaggc ttcgtccagc acgtcgttca tcgccagcgc
      781 gaaagcgtcg tgataggtca ggccgctttc ctgggcgatc ttctgtgccg tctccagcag
      841 tgggtaccag aacatggcgg caaacaagaa cgccgggttc acgcgcatat cgttatggat
      901 acgcgtatcg gtattcttca gcacctgttc aatgatccgc tccatcgggc tgtcgccatt
      961 ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg aacagatgat attcacacaa
     1021 cagcttatag gtttcgtaac cgtagcccgc ttgtagcagt ttaagcgatt cttcaaacag
     1081 gcgtgccggt gggatatcgt tcagcagggt agcgaggcga gggatcggtt ctgcggtttc
     1141 cgggctgatg cgcataccca atttggcggc aaaacgtacc gcgcgcagca tacgtaccgg
     1201 atcttcacgg tagcgcgttt ccgggttacc aatcagacgg ataacgccgt ccttcagatc
     1261 cttcataccg ccaacgtaat cacggacggt aaaatccgct acgctgtaat agaggctgtt
     1321 gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag
     1381 caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg ctgacgttac cttcgtggtg
     1441 tccacggaag gttgcaactt cgataatctc cgggccaaac atcacatgag ccagacggaa
     1501 acggcgaccc accaggcggc agttacggaa cagtttgcgc acctgctcag gcgtggcgtt
     1561 agtggttacg tcaaaatctt tcggcttttt gccaagtaac aggtcgcgca cgccgccgcc
     1621 aaccagccag gcttcgtatc ccgctttgtt gagcctgtac attaccttca gggcattttc
     1681 actgatatct ttgcgggaaa tagcatgctg ctcacgcggg atcaccgtca cctgtggacg
     1741 ggcgactgcc tgttcagcct cgctttcctc gcggcttagc accttgcggc aaaaattagc
     1801 gactcgggta aaaatagtac acctcggtag tgtcaaacat cattcaggac aaaaaaatag
     1861 cggctaatca tagctcagca tgacgcattt gagaatgttg aatttacaat tgccgactcg
     1921 ggcacggcgg taagccgcca gttgttgacg gctgactgaa ggatttgctc gacgctgaaa
     1981 tcctgccagt gtgcttctgc ctgctgcccc agaaattgaa gtgccgcgat tagtaccggg
     2041 cgtggatcgc ctttcggcaa cgcaggcgca tgattctgct tggaaagttt agcgccttgt
     2101 ggattaagcg ccagcggcag atgaatgtaa tctggcactt tccagccaaa aagctggtac
     2161 agcgagattt gccttactgt tggttcaatc agatcagccc cacgcactat ttctgtaacg
     2221 ccctggaaat gatcatcaac cacaacagcc aggttgtagg cgaacaaccc atcacggcga
     2281 tgaatgataa aatcttcccg tgccagtttt tcgtcggcgt gaataatgcc gcgcagctgg
     2341 tcagtaaatt gcgtgaccgg atgctgctgg cggatacgca ctgcggcatt gtctggtcca
     2401 tgatgcaaca cccggcaatg accgtcgtaa ataccgccaa tgctttgaat acgcgcacgc
     2461 gtacaggtgc agtaataact tagtccttgt tcatgtaacc aggcgagtgc ttcacgatag
     2521 gcgtggtgac gttgcgattg ccagagaaca tcgccgtccc agtgcagacc gtaatgttcc
     2581 agctggcgca ggatagtttc tgcggcaccg ggaacttcac gaggcgggtc gatatcttct
     2641 atgcgtacca gccagcgacc ttgccgggcg cgagcctgca aatagctgcc gagcgcggcg
     2701 atcagagagc caaaatgaag ctcgccggaa ggagaggggg cgaagcggcc aatatactgt
     2761 gtgtctgtca tctctttgaa caaaaaataa ggcgggagca tttcccgcct gtggtaaacg
     2821 tgatggaacg gctgtaatta gccagccatc tgtttttcgc gaatttcagc cagcgttttg
     2881 cagtcgatgc acagatcggc tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca
     2941 ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt ttttcagcgt cttctcgatc
     3001 tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc
     3061 tgggctgcac ggtctaccgg gtccgggaag ttggctgctt catcctgcat atgtgtaacg
     3121 gtgcgatcga cttcatccct gagttgatta cgccatgctt ccagaatacg acggaagtgc
     3181 gccagctggg cttcattcat atactcttcg cccggcttct cctgatatgg ttccacccca
     3241 gcgatggcga gaatactcag ggacgatgtt ttacggtttt gcccttcttg catgttgctt
     3301 ctccttaaca cgcactatcg atccccatgt tcgggggaaa aatgaggccg ctataaatag
     3361 cagatgcttt tccggatagc aattatctaa acgtaacact tgacaactgt gtgaggaaca
     3421 gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt acttacttta ctacaatgta
     3481 accggcagtg attttttaag agccatgcct tcagcagaaa tttccgcttt gtaagccaga
     3541 atttctaccc ccctctgttg agcttctgac aatagttgcg cgtatttctc atcgatgtgg
     3601 cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg cgaaaaagat aaccgcacgc
     3661 tggccttcag ccgctacgct catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc
     3721 gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag
     3781 cagtctggac gcgaatccgc ctgcaacata aagtcaatac ggctgcgttc tgcgccgtat
     3841 tttacttcgc ttttcagcga gctatagcct gacagttctg aaattgattc attaaggata
     3901 gcctctttcg tcaacctgtt agcccaaagc gtgttgacgc aaataaatgc gccgctctgg
     3961 ctttgagtta attcccaggt gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac
     4021 cagacggtat cgccaggcgt tgcacaaccg gtcattgcac ccgtattcgg gcagtgtagc
     4081 gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt
     4141 agcgtcgcgc gctgtagagg gggagagaat tccattgcga caatccttgt tattgcgtta
     4201 gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa
     4261 gggtgaactc cgtcaccgca taagaccagt taaaacctgg cggcgggatt gtcaccgcct
     4321 cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac
     4381 cgctgcgggc agcctgtgaa cggagcatat tcgccagctg gattaagccg cgtggaggct
     4441 gacgcatccc taaccacacc acacgcgaac gcagccattg tccggcgtca tcaagcgtga
     4501 gtgtgaaacc aggttgacga atccgtccgg ctaaaagaga aagcgccttc tctttctcgg
     4561 cgctcacttc gcctaaaaat gccagagtca gatgcaaatt atcggcggcg accggacgtc
     4621 ccgcctcagg tgggaagtgt gtggcgcgcc aatggataat ctgttcgcgg atttctgcag
     4681 gtaagtcgat agcaaagaac agacgttgcg gttcagacat gtgaggcact cggttatgaa
     4741 ttaccgcgat gctacaatgt ggcgcgaaga atgttaaccc tctggagcgt tttgtgtcgt
     4801 cgttgcccgt tgctgccgtc ttatctgaat tacttaccgc tctcgattgt gcgccgcagg
     4861 tattattaag tgcgccgacc ggggccggga aatcaacctg gctgccgctg caactgctgg
     4921 cgcatcccgg cattaacggg aaaattatcc tgctggagcc gcgtcgtctg gcggcgcgta
     4981 acgtcgcgca acggctggcg gagctgctta acgaaaagcc aggcgatacc gttggctacc
     5041 ggatgcgtgc gcaaaactgc gtcgggccga atacccgcct ggaagtggtt accgaaggcg
     5101 tgctgacgcg catgatccag cgtgacccgg aactgagcgg tgttggactg gtgatccttg
     5161 atgaatttca tgagcgcagc ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc
     5221 aacaaggtct gcgtgatgac cttaaactgc tgattatgtc ggctaccctg gacaacgacc
     5281 gcttgcagca aatgctgcca gaagcgcctg tcgtcatctc agaagggcgc tcgtttccgg
     5341 ttgaacgccg ttatttaccg ctgcccgcgc atcagcgttt tgacgatgcc gttgcggtag
     5401 ccaccgctga aatgctgcgt caggaaagcg gatcattact gttattttta cctggcgtcg
     5461 gagaaattca gcgtgtgcag gaacaactgg cttcgcgcat cggcagtgat gtattgctct
     5521 gcccgctgta tggcgcgttg tcgctgaacg atcagcgaaa agcgatcctc ccggcaccgc
     5581 aagggatgcg caaagtggtg ctggcgacca atattgctga aaccagttta accattgaag
     5641 gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc gcgttttgat ccgcgcacgg
     5701 ggcttacgcg actgattact caacgcgtta gccaggcatc catgacgcag cgtgccgggc
     5761 gcgccgggcg tctggagccg ggtatctgcc tgcatttaat cgccaaagaa caagcagaac
     5821 gcgccgcggc gcaaagtgaa ccggagatct tacaaagcga tctttccggt ttgctgatgg
     5881 aattactgca atggggatgc agcgatccgg cgcagatgag ctggctggat caaccgccag
     5941 tagtgaatct actggccgcg aaacgtctgt tacaaatgct gggggcactg gagggtgaac
     6001 ggcttagtgc gcaagggcaa aaaatggcag cgctgggtaa cgatccgcgt ttagcggcaa
     6061 tgctggttag cgcgaagaac gacgacgaag ctgctaccgc ggcaaaaatt gccgccattc
     6121 tcgaagagcc gccacggatg ggcaatagtg acctgggcgt ggcgttttcg cgcaatcaac
     6181 cagcctggca gcaacgtagt cagcaactgt taaaacgctt aaacgtacgt ggcggtgagg
     6241 cagacagttc gcttatcgcg ccgctacttg ccggggcgtt tgccgatcgc attgctcgtc
     6301 gccgtgggca agatggacgc tatcaactgg caaacggcat gggagcgatg ctcgatgcca
     6361 acgacgcgct aagccgccac gaatggttga tcgcaccgtt attattgcag ggcagcgcct
     6421 cgccggatgc gcggatttta ctggcgctgc cggtcgatat tgatgagtta gtacaacgct
     6481 gcccgcagct ggtacagcag tctgacactg tggagtggga tgacgcgcaa ggtacgctga
     6541 aagcctggcg tcggctacaa atcggtcagt tgacggtgaa agtgcagccg ctggcgaaac
     6601 cgtcagaaga cgagttgcat caggcgatgc ttaatggcat ccgtgataaa ggtttaagcg
     6661 tgctcaactg gacggcggaa gcggaacagc tacgcttgcg tttgttatgc gccgcaaagt
     6721 ggttgccgga atatgactgg ccagcggttg atgatgaaag tttattggcg acgctggaaa
     6781 cgtggctgct gccacatatg actggcgtac attcactacg cggcctgaaa tcactcgaca
     6841 tttatcaggc actacgcgga ttacttgatt ggggaatgca gcaacgtctg gatagtgaat
     6901 tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc cattcgttat catgaagata
     6961 acccgcccgc gctggcggtg agaatgcaag agatgtttgg cgaggccacc aatccgacga
     7021 tcgcccaggg gcgcgtgccg ctggtgctgg agttgctttc acctgcccaa aggccattac
     7081 aaatcacacg agatttgagc gccttctgga aaggagcgta ccgtgaggtg caaaaagaga
     7141 tgaaagggcg ttatcccaaa catgtctggc cggacgaccc ggcaaatact gcaccgacgc
     7201 gacggacgaa aaagtattcg taaggttggt tttctccctc tccctgtggg agagggccgg
     7261 ggtgagggca tcagcgcgca cgttcaccct caccctaacc ctctccctca agggagaggg
     7321 gaccgatcga gcacaaattt tgagagatat cttcttctgt cttgtaacag aagaacagaa
     7381 aatcgggctt ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc
     7441 caattggacg caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt
     7501 acgaagatga cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc
     7561 gcaaaggtaa gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc
     7621 tgctgttaaa actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg
     7681 atcaaaaaat tcgtagccgt attgatggca aggtctggca actgcctgcg gcagtttatg
     7741 gccgaatggt caatcttgag ccagatatga ccatcagcaa gaacgagatg gtgaagctgc
     7801 tggaggcgac tcagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc
     7861 aggccaacag cattgagatg atccgccgtc cgtttgattt cccggacagt aaagaagggc
     7921 aggtgcgtgc gcgtctgacc tttgatggtg atcatctggc gacgatcgtc aatatggaga
     7981 acaaccgtca gttcggtttc ttccgtcttg atccgcgcct gatcaccatg atctcttcgc
     8041 caaacggtga gcagcgtctg tttgtgccgc gcagtggttt ccctgatttg ctggtggata
     8101 ctttgctggc gacagaagac cgccatttct acgagcatga tgggatcagc ctctactcaa
     8161 ttgggcgtgc ggtgctggca aacctgaccg caggtcgcac ggtgcagggt gcgagtacgc
     8221 tgacgcaaca gctggtgaaa aacctgttcc tctccagcga gcgctcttac tggcgtaaag
     8281 cgaacgaagc ctacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg
     8341 agctgtatat gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc
     8401 cgctggcaag cttgtattac tttggtcgcc cggtagaaga gctaagcctc gaccagcagg
     8461 cgctgttagt cggtatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccgaaac
     8521 tggcgctgga gcgacgtaat ctggtgctgc gtctgctgca acagcaacag attattgatc
     8581 aagaactcta tgacatgttg agtgcacgtc cgctgggggt tcagccgcgc ggtggggtaa
     8641 tctctccaca gccagccttt atgcaactgg tgcgtcagga gctgcaggca aaactgggcg
     8701 ataaggtaaa agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg
     8761 acgcggcaga aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga
     8821 gcgatcttga aactgcgatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg
     8881 tcggaggttc tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga
     8941 ttggttccct tgcaaaacca gcgacttatc tgacggcttt aagccagccg aaaatctatc
     9001 gcctgaacac gtggattgcc gatgcgccaa tcgcgctgcg tcagccgaat ggccaggtct
     9061 ggtcaccgca gaacgatgac cgtcgttata gcgaaagcgg cagagtgatg ctggtggatg
     9121 cgttgacccg ttcgatgaac gtgccgacgg taaatctggg gatggcgctg gggctgcctg
     9181 cagttacgga tacctggatc aaactgggcg taccgaaaga tcagctgcat ccggttccgg
     9241 cgatgctgct gggggcgttg aacttaacgc caatcgaagt ggcgcaggcg ttccagacca
     9301 tcgccagcgg tggtaaccgt gcaccgcttt ctgcactgcg ttcggtaatc gcggaagatg
     9361 gcaaggtgct gtatcagagc ttcccgcagg cagaacgcgc tgttccggcg caggcggcgt
     9421 atctgacact atggaccatg cagcaggtgg tacaacgtgg tacgggtcgt cagcttgggg
     9481 cgaaataccc gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata
     9541 cctggtttgc aggcattgac ggcagcacgg tgaccatcac ctgggtaggc cgtgataaca
     9601 accagccgac caaactgtat ggagccagcg gggcaatgtc gatttatcag cgttatctgg
     9661 ctaaccagac gccaacgccg ctgaatcttg ttccgccaga agatattgca gatatgggcg
     9721 tggactacga cggcaacttt gtttgcagcg gtggcatgcg tgtcttgccg gtctggacca
     9781 gcgatccgca atcgctgtgc cagcagagcg agatgcagca gcagccgtca ggcaatccgt
     9841 ttgatcagtc ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga
     9901 aagacagcga cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacatctaa
     9961 gcgtgaaata ccggatggcg agttgccatc cggtaaaata acatcccatc taagatatta
    10021 accctttctt ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc
    10081 ttgcgaaccc
//
LOCUS       DAPPNJ010000162        10078 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000162 DAPPNJ010000000
VERSION     DAPPNJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.163"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(95..712)
                     /locus_tag="RPM73_003440"
     CDS             complement(95..712)
                     /locus_tag="RPM73_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDX6521766.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            979..2478
                     /gene="ansP"
                     /locus_tag="RPM73_003441"
     CDS             979..2478
                     /gene="ansP"
                     /locus_tag="RPM73_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDX6521767.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2593..3654)
                     /locus_tag="RPM73_003442"
     CDS             complement(2593..3654)
                     /locus_tag="RPM73_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDX6521768.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3896..5998
                     /locus_tag="RPM73_003443"
     CDS             3896..5998
                     /locus_tag="RPM73_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDX6521769.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6034..6699)
                     /gene="mcbR"
                     /locus_tag="RPM73_003444"
     CDS             complement(6034..6699)
                     /gene="mcbR"
                     /locus_tag="RPM73_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDX6521770.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6897..7934)
                     /gene="curA"
                     /locus_tag="RPM73_003445"
     CDS             complement(6897..7934)
                     /gene="curA"
                     /locus_tag="RPM73_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDX6521771.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8115..8633
                     /gene="mddA"
                     /locus_tag="RPM73_003446"
     CDS             8115..8633
                     /gene="mddA"
                     /locus_tag="RPM73_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDX6521772.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8630..9079
                     /locus_tag="RPM73_003447"
     CDS             8630..9079
                     /locus_tag="RPM73_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX6521773.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9080..9313)
                     /locus_tag="RPM73_003448"
     CDS             complement(9080..9313)
                     /locus_tag="RPM73_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDX6521774.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9399..9572)
                     /gene="ortT"
                     /locus_tag="RPM73_003449"
     CDS             complement(9399..9572)
                     /gene="ortT"
                     /locus_tag="RPM73_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDX6521775.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9767..9862
                     /gene="yncL"
                     /locus_tag="RPM73_003450"
     CDS             9767..9862
                     /gene="yncL"
                     /locus_tag="RPM73_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDX6521776.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2436 a         2464 c         2448 g         2730 t
ORIGIN      
        1 aaatcaaaaa acataacatg aatttaatta attacaccca tcattgtgga taaagttact
       61 gaataataaa taacataatc ttctggtatc ccactcaaat aatttcattc ctttttaata
      121 cttcttgtaa tttcggtaac tgacatacag catcagcgat cgcgctgata ttctgcgcat
      181 tatcctgaaa ccattcatgc cccggccccc atgtgcgcat ggtgcagaga taacagtcca
      241 ccagcgttag ttgctcacca aaggcataag gttcagcggt caattgtgaa ttcagccaga
      301 tataaagtga tttgcgatat tcaattacat tcttctttaa ctgttcaggt gcgtcagatg
      361 cccaacgctc agggtaatcg gcgaaagtga atgttggata gacattagca accagccaga
      421 ccaatagccg ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc
      481 gacgatcgag aaccatcaat gcaatcgccg ctgtctccgt cataatctcg tcattttcca
      541 gcgccagtgt cggaacctga cacaacgggt tcagggtttt caataactcg cgtgaagctc
      601 cctcgtggtc aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca
      661 tcagctcact gattgtagag ccccatccgg gtacgccata caccttaatc atatgcccct
      721 cacaatcttt taaagattaa gtgtaggaca gtacacaggt taaccagcaa aaggatttgt
      781 cattttttcg tcagttgtat taatcccctt caggattgtt aggggatgtt ttgcatcgtt
      841 tatcgataac gggtaggatg ctgcgccgat agttttatac ccataaagaa taatggtgat
      901 aactatcatc gccaggatga ataaacattg ttcatggcaa cttatatgac tttttcatta
      961 aagcaatcag ggagagcaat gagtaaacac gacaccgaca cttcagatca acacgccgcg
     1021 aaacgccgct ggcttaatgc ccacgaagag gggtatcaca aagcgatggg caatcgccag
     1081 gtacagatga tcgccattgg cggcgcgatt ggcaccggct tgtttttagg tgcaggagcc
     1141 cgactgcaaa tggcgggtcc agcattggca ctggtttatt taatttgtgg cttgttttcg
     1201 ttttttattc tgcgtgcatt gggtgagctg gtgctacacc gcccttccag tggcagtttt
     1261 gtctcttatg cccgtgagtt tttgggtgag aaagccgctt atgttgctgg ctggatgtac
     1321 ttcatcaact gggcgatgac ggggattgtt gatattaccg ccgtcgctct gtatatgcat
     1381 tactggggtg cgtttggcgg cgtgccgcag tgggtctttg cgctcgctgc acttaccatc
     1441 gttggcacca tgaatatgat cggtgtgaaa tggtttgcgg agatggagtt ctggtttgcg
     1501 cttattaaag tgctcgccat tgtgaccttt ttggtcgtgg gtacagtgtt cctcggtagt
     1561 ggtcagccgc tggatggcaa caccactggc tttcatttaa tcaccgataa tggcggcttc
     1621 ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg gcgtagtgtt tgcttttgcc
     1681 tccattgaaa tggtgggtac agctgccgga gaatgtaaag atccgcagac catggtgcct
     1741 aaagccatta acagtgtgat ttggcgtatt ggcctgtttt acgtcggctc cgtggtgttg
     1801 ctggttatgt tattgccgtg gagcgcgtat caggcggggc aaagtccgtt cgtgacgttt
     1861 ttctctaaac tgggtgtgcc atatatcggc agcattatga acattgtggt gctgaccgct
     1921 gccctctcca gcctgaactc aggtctgtac tgcaccggac gtattctgcg ctcaatggcg
     1981 atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc gtcagcatgt gccgtatgcc
     2041 gggattctgg cgacactagt tgtgtatgtc gtcggcgtat tcctcaacta tctggtgccg
     2101 tcgcgcgtat ttgagattgt gttgaacttc gcgtcgctgg gaatcatcgc ttcatgggcg
     2161 tttatcatcg tgtgccagat gcgcctgcgt aaagcaatta aacaaggcaa agcagcggat
     2221 gtcagtttta aactgcctgg cgcgcccttc acttcctggc tgacattact gtttttactg
     2281 agtgtcctgg tgctgatggc gttcgattac ccgaacggga cttatactat cgcggcgctg
     2341 ccgattatcg gtattctgct ggttataggc tggtttggtg tgcgcaaacg cgttgctgaa
     2401 attcacagca ctgcgccagt cgtcgaggaa gatgaagaaa aacaggaaat tgtgtttaag
     2461 cctgaaacgg cgagctaatt gcaaaattgc ccggctacgt gccgggcaaa tttagattac
     2521 tgacgaaggt gtgccgcatg cggcgtgata aaagtcaggc aagtctctcc gttttcaccc
     2581 tcgccacatc tattacagcg caatacgaat cacatcgtcc ggctgggtag cttcctgctg
     2641 tttagtggat ttttgtttca cactgacata cagcgttttg ccatcggcag acagcgccag
     2701 gctgtttgga tgagtcggcg tatcgaacgt tttcaccact ttatagcttt tcgcgtcaat
     2761 cacactgact ttacctgcct gacgatgcgt tacgtaggct tcattacgcg ctgggttaaa
     2821 cagcacagcc agtgattctg gtgccgcaac cttcgccaga atattgccat tacgggtatc
     2881 gaccactaac acttctgcgg ctttagaatc ggtgataaat gcacgctgcc tggcggtatc
     2941 aaggctaatg ttgataaaga agtgctcttt gccgtcatcc agcagctttt tacggctgag
     3001 gattttattg tcggcggtgt cgatggtaat caattcgccg tcagcgttag tggtgtaaag
     3061 acgtttgcct ttgctatcca gcgccagacc ggtactcatt ttaccggtgt tctggatggc
     3121 ggttttcagt ttgatattct cgccatcaac gacccaaatc acgctctctt taccaatacc
     3181 actgatgtaa acggtgttcg tggcatcatc agctaccagc tcacgcggtt gcagcgggcg
     3241 cacctcttcc gtgcgcttac gatcatccag caccagacgg cctttcacct cgcccgtttt
     3301 ggcatctatc gccgtgaccg cgctgtttac ggtgttacca aaccacaacg tctgagtcgt
     3361 gttattgatg gtggcaccaa acggcttgag atcgttatgg atcgcctgcg tcacttccag
     3421 agtgaccgga tcaagacgat aaaccacgcc acctttatcc agtttgcggc tttgcgaagt
     3481 ggcgagccac agtgcgtttt cttgctggct ataagccatt tcgtaggcac ctttacctac
     3541 cgctttacgc agcatttctt ctgcggcctg cgtactgaat gatgaagcaa caagcaatga
     3601 acctaacagt aatgaaccac gcaggcgcga tgaaaacaga tgacgtaaat gcatgacgac
     3661 tccctttgat gaaccgatat ttttgctgct tcacaatgct tcgctaagcg taatcatcgt
     3721 tacgctcttg ttataaatgg gaatggtaat cattattttc tcaaatgtgg agcatttctt
     3781 gccgttacga ctacaatttt ctcaattact taacgtatcg tgccgtttcg ttttccataa
     3841 atttacaatt tcattaccat aacggcgttt tcccattctt aacctggctt ttttcatgaa
     3901 gattttttcc gtccgacaga ccgttttgcc cgcactgctt gtcctttccc ccgttgtttt
     3961 tgccgctgat gaacagacta tgattgtcag tgccgcaccg caggtggttt cagaactgga
     4021 taccccagca gcagtaagcg tggtggatgg cgaggagatg cgcctggcaa caccgcgcat
     4081 taacttgtcc gaatcactga ccggcgtgcc tggtttgcag gtacaaaacc ggcagaacta
     4141 tgcgcaagat ttacagctgt cgattcgcgg atttggctcc cgctccactt acggtattcg
     4201 cggtattcgc ctgtatgtgg acggtattcc cgccaccatg cccgacgggc aagggcaaac
     4261 atccaacatc gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc ccttctctgc
     4321 cctgtatggc aacgcgtctg gtggggtaat gaatgtcacc acccagaccg gacaacagcc
     4381 accaaccatt gaagccagta gttactacgg cagttttggc agctggcgct atgggctgaa
     4441 agcaacgggc gcaacgggag acggcacaca gcctggcgat gtcgattaca ccgtctcaac
     4501 cacgcgtttt acgacccacg gctatcgtga ccatagtggc gcacagaaaa atttagctaa
     4561 tgccaaactg ggcgtacgca ttgatgaagc cagcaaatta agtctgattt tcaatagtgt
     4621 ggatatcaaa gcagatgacc caggtgggct aaccaaagca gaatggaagg ctaatccaca
     4681 acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa ctcaggctgg
     4741 gttgcgctat gagcgtagcc tgagcgcgca agatgatatg agtgtgatga tgtatgccgg
     4801 agagcgagaa acgacccagt accagtcaat cccgatggcc ccccaactta acccgtcaca
     4861 tgcgggcggc gtgattaccc tgcaacgcca ttaccagggg atagacagcc gctggacaca
     4921 ccgtggtgaa ctgggcgttc cggtcacgtt cactaccggc ctgaactacg aaaacatgag
     4981 tgaaaaccgc aagggctaca ataacttccg cctgaatagc ggcatgccgg agtacgggca
     5041 aaaaggtgag ttgcgtcgcg acgaacgcaa tctgatgtgg aacatcgatc cctatttaca
     5101 gacgcagtgg cagctgagcg aaaaactgtc gctggatgct ggcgtgcgct acagctccgt
     5161 gtggtttgat tccaacgacc attacgttac tccgggtaac ggcgatgaca gcggtgatgc
     5221 cagttatcat aaatggctac ctgccggttc gttaaaatat gcaatgaccg atgcctggaa
     5281 tatctatctg gcagccgggc gaggttttga aacgccgacg attaatgagc tgtcttatcg
     5341 tgctgatggg caaagcggta tgaactttgg tttaaaacca tccaccaacg atacaattga
     5401 gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt ttcagaccga
     5461 cactgatgat gaaattgttg tcgatagcag tagcggtggg cgtacgactt acaaaaatgc
     5521 cggaaagacc cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg caggagattt
     5581 tcgcgtaaac gcgtcctgga cctggcttga tgcgacctat cgcagcaatg tttgcaatga
     5641 acaggattgt aacggtaatc ggatgccagg gatcgcccgt aatatgggct ttgcgtcgat
     5701 aggttatgta ccggaagatg gttggtatgc aggcacggaa gcgcgttata tgggcgatat
     5761 tatggcagat gatgaaaata cggcaaaagc gccgtcttat actctcgtcg gcttattcac
     5821 cgggtataaa tacaattacc acaatttaac tgtggattta tttggtcgtg tcgataattt
     5881 attcgataaa caatacgttg gttctgtcat tgtcaatgag tcaaacgggc gatattacga
     5941 accttcgccc ggacgaaatt atggtgtcgg catgaatatt gcgtggagat ttgagtaatt
     6001 accttgatgc ccggtatttg ccgggcattt actttaacga ttgtattgct ggtataaaat
     6061 agcaatattt tgttgcataa tttcagcaag acaatgtctg catccctctt tgtcttttgc
     6121 ttttaacgca gcgagtaaca gatgatagtt ttctatatgc tcccgtaatt ccgctggatt
     6181 aatcgcttca tagagataat gtaaaccagg ccccatcctg acccacagtt gctcaatcat
     6241 ctcacacagg atgggcatat ttgagcgatg ataaatcgct aagcgaaata gcctgtttac
     6301 attaatgatt tgttccatgt cgcccttttc ttgcgcctgc tgtaatttct cgagcaattc
     6361 ctggagttcc gcaaggtctt gcggggtgag gttttcaaca gccagagcaa ctgccattaa
     6421 ttccagttcg tagcggatcc gattgatttc gtctaattga cgtttcccca cttccggaac
     6481 tgtaaatgct tgtgcaggtg cgacagaaag cgcattcacc gaaaccagac gtaataatgc
     6541 ttcacgcaca ggtgtaatac tcatacctaa ttgctccgcc agatttttag taatcaggcg
     6601 tgcgccaggt tttaatgcgc caatactaag ctgatgtttc aggtcgttct caacctgcag
     6661 agtcaaactg acatgtttca tttttcccat tccaggcatt ttaattcctt cgcgtgtttt
     6721 ctgtccaacc ctgcctgcac ggcagaagga gttcacctgg tgtattaaag tgaataatca
     6781 taaaccaccc acctttgttg ttattagcag cagaacaatg acgtggttag ttaattaaag
     6841 ctgaaaataa gctttttgcc tggattttta tggcggctta cgccgccgtt gaaactttaa
     6901 tcatcacccg cgacgcggat caccactttg ccgaagtttt tacccttcag caggccgata
     6961 aacgtctgtg gcgcattctc taaaccatca gtaatatctt cgtggtagtg gattttatcc
     7021 tctttcaccc attgccccat ctccttctga aactcatgga tgcggtgacc ataatcctga
     7081 gcgataataa acccttgcaa gcgaatacgt tttttcagca ccgttgccat caacaggggt
     7141 aaacgatccg gaccgggtgg tagctctgta gcgttatagc tgctcactaa gccacaaacg
     7201 ggaatgcgcg cagatgtatt aagtaatggt agcactgcat caaatacctt accgcccacg
     7261 ttttcataat agatatcaat accttttggg cacgctttca ccagttgttc ggcaaaatca
     7321 tccgcgtggt gatcaagaca aacatcgaag cccagcacct cgatagcatg gcggcatttt
     7381 tccgcgccac cggcaacccc caccaccctg caacctttaa gtttgccgat ttgccccacc
     7441 gttgctccca caggacctgt cgccgcagct accaccaacg tttcgccctc tttaggctga
     7501 ccgatatcca gcagccccat ataagcggta aagcctggca tccccagcac acccagcgac
     7561 cacgatggat tttgcggatg atcgccaagt ttcaccagat catcaccact ggatatgtca
     7621 tagtcctgcc atccactgta gcccagcacc cagtcgccag gctgataatc aggatgattc
     7681 gactccacaa cacggctcac cgtgccgccg accatcacgc cgccaatatc aacaggcggt
     7741 gaataagatg gctcatcgct catacgtcca cgcatatacg ggtccaggga caaataaact
     7801 gtgcgcagta acacctgtcc ttcacccggt gtggcgacat catcttcttc aagacggaaa
     7861 ttctccggaa ccggtgcgcc atgtggacgc gaggccagaa cccaacgtcg attacgctgc
     7921 ttttgttgcc ccataatgct ctcctgtgct tttggcatga atccatcaat agtagcaggc
     7981 tcacgggatt ggttttgttt gcagccgtca catcccctct gcaacacgca ataatttcat
     8041 cgaggaatcc gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt
     8101 tatcgaggcc agacatgtcc atccgttttg cccgcaaagc cgattgtgct gccattgcgg
     8161 aaatttataa ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggttgatg
     8221 cagataaccg cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtga
     8281 gtgaagaaga tggcgtagtg acgggatatg cctcgtttgg cgactggcgt agtttcgatg
     8341 gttttcgcca taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc
     8401 tggggcgtaa attgttaagc cgattgattg atgaagcgcg ggattgcggg aagcatgtca
     8461 tggtcgccgg gatcgaatcg caaaatcagg cctcgctgca tctgcaccag tcgctgggat
     8521 ttgtcgtcac cgcacaaatg ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga
     8581 catttatgca gttgcaactc gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc
     8641 gctcaccctt gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat
     8701 ggtgcgcatc acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt
     8761 ggggattgtg ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga
     8821 actggtgtcc agcgtgcgct ggtggacact tatacccggc ttgctgggat cgttttttgt
     8881 cttcgccagt atcagcggat atcagaatgt gggagcggcg acaactattg cagtactggt
     8941 cgccagccag ctaattggcg gtctgatgct ggatatcttc cgcagtcatg gcgtaccact
     9001 acgggcgttg ttcggcccaa tttgtggcgc aattttactt gtggttggcg cctggttggt
     9061 agccagacgc tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg
     9121 cttccatatc ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct
     9181 gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt
     9241 cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat
     9301 ccagatgcga catactttct ccttaaccac ttgtgaaatt tatcgattca tccaaatgat
     9361 ataagtctgc gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc
     9421 gccaccggga aactcatcgg ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg
     9481 cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca
     9541 taaaaaacaa gcatatgttg atagagagac atgtacgggc gtccagccag atgaataaaa
     9601 cgcgcgcaaa tatgcgtcat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa
     9661 aatcatcaaa cagcaacttg cccttattca tcttcagaat gagtattatt gcggattttc
     9721 ttaacccgta ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag
     9781 tagaactgta gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat
     9841 gagatttggc tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa
     9901 tcaactctct gacagtacgc aagatcttct catactctcc aggtttacaa cattatcctt
     9961 aatatttact tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg
    10021 tcgtctatac aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatc
//
LOCUS       DAPPNJ010000163        10018 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000163 DAPPNJ010000000
VERSION     DAPPNJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.164"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..851
                     /locus_tag="RPM73_003451"
     CDS             <1..851
                     /locus_tag="RPM73_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDX6521777.1"
                     /translation="EVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITQQYGTPD
                     LCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQNLRAPVPMTW
                     AFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQERTFSLSIAE
                     NLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRSAAIIKDAYIA
                     ITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALK
                     SVLARI"
     gene            complement(1113..8188)
                     /gene="yeeJ"
                     /locus_tag="RPM73_003452"
                     /pseudo
     CDS             complement(1113..8188)
                     /gene="yeeJ"
                     /locus_tag="RPM73_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(8502..8577)
                     /locus_tag="RPM73_003453"
     tRNA            complement(8502..8577)
                     /locus_tag="RPM73_003453"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8542..8544),aa:Asn,seq:gtt)
     gene            complement(8678..9475)
                     /gene="mtfA"
                     /locus_tag="RPM73_003454"
     CDS             complement(8678..9475)
                     /gene="mtfA"
                     /locus_tag="RPM73_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDX6521778.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            9569..9658
                     /locus_tag="RPM73_003455"
     tRNA            9569..9658
                     /locus_tag="RPM73_003455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9603..9605,aa:Ser,seq:cga)
BASE COUNT         2434 a         2377 c         2417 g         2790 t
ORIGIN      
        1 tagaagtctt taatgcgctc aaacttgaac agactaaatt aacattagtc acaatgacta
       61 tgcaggatca cgcaacatta aaagaaataa aagatttagc gaagaacata acacagcaat
      121 acggtacgcc ggatctttgt atagaggcga tgcgtaaaaa gaacctgaaa acgatgttat
      181 ttatcagtca gttgaaagca aaaacgaatt ttcaggttgt tggtttaacc atgaagtgct
      241 attcccctgt gtgtaagaac gcgtcccgga tggatcagaa tctccgggct cccgtaccca
      301 tgacatgggc atttatgatg cgtgaggcgg gttttccagc agtcaggcca atatatgaat
      361 tgccactaag tgaggatgta ctcgatgagg tacgcgagga aatgcgatcg ttaggatctt
      421 acattgcgct taatttagaa ggtagctcgc aggaacgtac attttcacta tcgattgcag
      481 aaaatctaat agcaaaaatt caaagtgaaa cagatatacc aatagtgatc gttcatggac
      541 caaaagggga agataaagcc agggtattag tggattgtta taataatgtc taccgtttat
      601 ccttatcacc ctcgattaaa cgttcagcag caatcataaa agatgcttat atcgcaataa
      661 ctcctgacac ctcaatatta catatggcaa gtgcctataa tacccccgtt gttgcaattt
      721 atgctgatta caaaacgcga tggcccgcaa tggccgatgt ttcggagtca gtcgtcgttg
      781 ggcaaaaaat tgacaatata agtctggatg aattcgcaaa ggcattaaaa agtgttttgg
      841 cgagaatatg agacagtact tatgcatagt aatgccgatc tgttatgaac ggtggtgtca
      901 tcaatactct gaggctggtc aatcatcaca gaaaaccagc ctttggatca aagcagtact
      961 caccggatac gatcatagtc acaccgaacg cgattcttgc ggtgcagcat ttttatttat
     1021 tcattttctg tgagagaagg gcaagctcta tagagggaat gtaaaaaaga gtgctccgaa
     1081 tggagcactc tttaagaaat tataacggaa aatcagaggt ttttataaca tgtcgcataa
     1141 gcaaacccaa gcttttcaaa tacgctttga tcacctgttg ccagggaaac tacgtattgt
     1201 tcgtttgagt ttgcgggact agatgaccaa tacatatttg attgaaaacc agcttcagtc
     1261 gtgtaatgcc ccatatctcc ccattcactg tacagtgacc cgatgcctcg ggaactactg
     1321 tggtttaaat agtttgctct ggggagcgtg tagccattgc tgctgcaaaa attttcagca
     1381 aggctgtata tcatgaaagc ctcgccggcg ttcacccacc aactcttcac acggatttcg
     1441 tatacatagc tagggcctcc tgattttggc gtcgccgtaa tcctttccca attgctgccg
     1501 acatttttaa atgtcacttt acctgtagca tcaacatcga cccaggaggc agagcttgaa
     1561 aactcataat cagccgccgt ttttcctggg gcaaagttgt cattattcaa ctgataatac
     1621 gccccggtga acccctgcga agggaatgta gttgtcggta ggtcagtacc attgactgat
     1681 actgtaccgc tcattatttt gtcttctgcg cgagtgaatt gtattgtggt actcagacca
     1741 gcttgatgaa caccattcaa tacagggatc agcgttgcga taccctctcc gccgcctgta
     1801 atggtggctt tgtaatcacc gttgatattt tgactgtaat caattgcgct aattttcata
     1861 tagggcacgt tagtacctga ttgcacaaat tccatccctt cagaaacttt gatcggattg
     1921 cctgatatat cgtgcaaaac aagatgtagc tcagcactat cggtaaagtc ccctttcagt
     1981 gaggaccgat tgttcttaag gacggactgc gaggcgtctg ccgggcctgc caccagcgtt
     2041 atatctactg tttgcattcc accagcggtc gtgactgcgc ttaccgtgac gctacctgtc
     2101 atcgctcctt tcacgcttgt tgtcgcgatt ccgttttgat cggtcaccgc tgttaatgac
     2161 gttaatgtgg cagagccgct ttttaaggcg aagtacacag tgagaccttc gatcaggtta
     2221 ccactgccat cctcaaccgt tgcctttaag gtacttaaat cactgttggt ggcggcgata
     2281 gtcgatggat cagcgataaa gctagcaaca tgtgcggtgc ttgagttgcc agtgactttc
     2341 acggttgtct gtgtctgtat tgttacgccg ttatggaacg atgcagtcac cgtatatgta
     2401 ccgactttat tgctggtcag aacgaccgga gcctgacctg aagagttagt tctcactttt
     2461 ccgccactta acgtcgcccc ttctggcgtt acgctaaagt tgatgacctg accctccact
     2521 ggagtgccat ttgcagaggt tagcgttgcc gtcagagtag tgcctgtagg ggcatagaca
     2581 ccgataagcg gactggacgc cgtgagtgtc agtttttcat caatagcctc cagttgtttc
     2641 tcaagtgagg ctccattcgc cagggatgct ttcaccatat acgaaccgtt tctttcgggc
     2701 gtcatggaga cctcggctat accatgcgta tcagtagaga caatattttg gctgatggtc
     2761 atgtgctctg gtggctctgc actgaatgtt acagattcat taagaatcgg gttgccaaac
     2821 tggtcgttga cgtgagcttt aactgctacg tcttgtgcga ccattagctg accttcaggt
     2881 atctccagac tggtaatcgt tgcagaatta atatctgctt ttgcattcag taatcgcgat
     2941 atgacttcag taggtttatc tgccagagag gctgaaactg ttttcagtcc gacttcggtg
     3001 cttgtcacaa gaatttcagc gaaaccctga tcatccgttt caacgctggt gctgcttagc
     3061 gtgacggagg ttccgcggaa attaactttt atgccttcta ccgggttgcc gttagcgtca
     3121 gtgacgcttg cagtcatggt cgcgccctcg gtcgtgctga cgacgaatga gtatacagag
     3181 gttaaggagg ctagtgttgc ggtaccagca tcggcaatca aagtcacctg tttcgtatca
     3241 ctgacaccat aattgttcac gctaactgtc acggggtagg taccggattt tgtaccgctc
     3301 agtgtaactt cagccttgcc gttgatatca gtaatggcgg aaccgccttg tccgagagtg
     3361 aagcttgcgc tcacgtctgc cggtagcgtg aatgtcaccg cctcattggc taacgggttg
     3421 ccgtttccat cagtcactgt tgcctgaagt gtggtcatcc caacgttatt ggcgataaag
     3481 ttgtcctcgg taacgttaag attaacctgc gcactgagcg tatccgcaat aaagttcacc
     3541 accaactgct cactcttacc gccagtcatc gatgctgtaa cagtatgagc gcctgctttt
     3601 gtacctttca gcgtgacttt cgctttgcct tcagcatcag taatcgcttt accgccatcg
     3661 ctcagcgtga agttcgcctt cacatcttcc ggcagagtaa atgttacttc agtgttggct
     3721 atcgcattgc cctctgtatc agcgactgtt gctgttagtg tcgtgagatc gttattgtcg
     3781 gcaatcaccg gatccttcga ggctgccagc gtaatttcgg cattcgcgac gttcggcaca
     3841 taggtcactg tttgttgcat cgaatcgcca ttttcgaggg ttgccgtcac ttgataaacc
     3901 ccggcttttg tagcggtaaa gcttgcgtaa aaattgccgt cgtggttggt agtatatata
     3961 gcgttattac tggggggcac gccttcactt ggtgatactc tgagggttac ctcctgctgg
     4021 ggtacaccgt tgccgtaatt atccttcacc tcaatataca gattggtagt gtcgcctgcg
     4081 gagactgtat caaaaagtgc ctgtagcaag gtgagatgtg ccgtagacgc atcagcgttg
     4141 acattaattg atgtgctaag cgtggagcta ccattttcca gactggcctc aacggtatcc
     4201 ggtctcgctc ctgattctat cgaggagcgg gtattggtat aagtgacggt agccttaccc
     4261 tgttcgttcg ttacggcttg gccgccattc gtcatttcgg cagagtttgt tctgctggtg
     4321 aagttcacag tcacaccttt caccggaaag ccattattat caacgactgt ggcggtgatg
     4381 acgctgccgg agctgttctg cggggtaccg gcgattatgc tgtctgggac aggcgtcaac
     4441 tcgataattt ttgccgctgc tgtgtcgcca ataaaatgca cagttttgtt gtcgctggca
     4501 ccattattag ccagtgatgc agtgaccgtc tgctcaccaa aggcaacgcc tgcgagagtg
     4561 gcctgcgcga tgccagactc attggtatta cttactcccg gggtcagggt gagtcctgaa
     4621 gaggctgagc tgaatgttac cggaagatta ttcacctcat tgtcgaactg atctttaacc
     4681 gttgcagtta gcgttgcgtt atcgacgcca ttacctgtga tctcatcttt tgacatctgc
     4741 aggacaacct gagccgaggt tttgtccgcc acaaacgtta ccggctgcga atcactggta
     4801 ttgttattac ccagcgttgc ggtaaccgta tgcgtacccg cttttttacc tttgagcgtg
     4861 acatgcgctt ccccattggc ttgggtgatg gcaatgccgt tattttcgag ggtaaagttt
     4921 gccgcaacgt cctgtggcat ggtgaaggtc accgttattc ccgccaccgg atgattcgac
     4981 ggatctttca ctgttgctgt cagagtcgtc tcatccacgc cattcccaat gatttccgct
     5041 ttcgatgttt gcagaacgac aaccgctctg tctttatcgg ccacaaacgt cattggctgt
     5101 gtatcgctga cattgctgtt agccagacga gccgtgatca tatgcgtgcc cgctaacgtg
     5161 ccggtcaggg aggcgattgc agtcccgttg ctatccgtca tgccttttcc gctgttgcta
     5221 atcgagaacc gacttgcgac gtcgtttggc acagagaagg tgatttcttt atcttttaat
     5281 gggttgccat ttttatcctg caaggttgca gtcatatgta gcggagctgt gttggtgacg
     5341 gtgatatcac ctgaaggcac actgagggtc agggcagcag tactttgatc accgataaaa
     5401 atcacctgtt gattagcctg agaaccagag ctcacagagg ccgtaaccct ataatcacca
     5461 tttttcaaac tggtcagcgt agctgtggcg atcccgtcgt ggctattcac ttctgtttgg
     5521 ctcagtttcg cctctgctga attaacattg aaagtgacct tgacgtcatt gagcaggttg
     5581 ccttttgcat cccggactgt cgcggtcatt gtggcgctat cattgccgtc agcgaccact
     5641 tcatttttcg acttctgcag ctcaacctgc gcggtactcg agtcgccgac aaaactgacg
     5701 attaacgttt gtttcacgcc attttcaagg gtgacttcaa ccgtgttgtc ttcctgctta
     5761 ctacttttca gatcaaaagt cgccagacca ttaacatccg tttttgcggt gttttgattg
     5821 ttgaaggaag ttgccgatcc gcttaatacc gcaaacgtga cggtatgatc attgattggg
     5881 ttgcttcctt catcagcgac attgaccgag acggtgtttg ctgcattctc attggcgagc
     5941 acaccattat tgctggcaga taatgtcgca atttttgctg actgcgggtt ggcgtcgatg
     6001 ataaaaccag cggtatgcaa atcttcattc cagttttgca ttaatagctt cgcagtaagc
     6061 ccactgcctt tggtataggc ggtataggtt gccttataga cgccatctgc ggtttctttc
     6121 cagtctgtag tgacaccagg tttcacgttg tcgatgctga ctgcgttatt cagttgctgt
     6181 ttttgctcct taacaggttt gtcattttca tctctcagtt ctaccgtcac ctcgatagga
     6241 ttgccggaga gataacggtc tttatcaatt ttaattgacg agtgagttcg ggatgacgaa
     6301 atagaaatga tattcaccac ggcgggggct ttagccgcat ccacaccatt cagctgtggc
     6361 atcagcgtca gcgtgccaga catcgctcct gtggtcagga tctgggtata gcttccgtca
     6421 ccattatctt tccattcaga aagggtgatg tcctgaacac cttcgtgacg cgtcgagagc
     6481 accagcccga caacaggatt acctgctgca tcatgcgcaa taaaagtcag tgtggcggtt
     6541 gaatgggaat ccgcgttcaa tgtttgggta cttaacgata ccgaggaatc tttctggctt
     6601 agcgtaggtg cctgaacgac caccatgctc tgttcacgat tcgacaaatt gcctttgaca
     6661 tcttcggcgg tgacttcaat cggccagtgt tatcggtttc tggcgtactg gtgaaccggt
     6721 aagccggcag ggtaaccaga atatctttac ccgttgtgac cactttgcca ccggcagctt
     6781 ccagagcggt ggcttcgacg ttatagcctt tcagggcata tttggtttgt agcgacgaaa
     6841 ccaatgattt cacttctcct gacttccctg tcacggggtc tgtcagggtc aggcgaacca
     6901 gttctttttt gcggtattcc agaacgatgt tgttgttgcg atccaccaga tcataacggc
     6961 tgcctgcaag gctacgccgt gcagcgactt cgttcgggtc aagctgtttc tgcattgcgc
     7021 tgccaggttg ccaggtaaaa tcgacggcaa aacgggtgtc attttcgccc tgtttaccct
     7081 ggcgttgctc cgcgctgaag gtcatcagcg ggaagggggt atagttaagt ccagcggtta
     7141 tggcatgagg attactttgc cgatcgtctt tatcgaacag ggccacttca tcgccataat
     7201 actgttcata gaccagttta ccgccaaggt gcggccaggc gggtagccag ccttctgcgc
     7261 gtacatccca gccattggcc gggcgggctt cataatcgtt gtccagctca ggtgcgctgc
     7321 gccagttggt cagtcgcaaa tagccgttac tgcttaattt tagatagtcg cgccagtact
     7381 ccgcgccaat gccggcgcgg gagtggtaac ggctaagatc gtggtcgaaa aagaagttga
     7441 tgcccgacat ccatgtggga gtgaaatgac gccaacctaa gccgttgtta atctgcgtac
     7501 gctcgtcagt acgatggaga gtatgctgac tgaaaaagag attatcaggc gtttcatacc
     7561 acggatggag aaaatcgaac tgggagttct tcaggctaaa atcttcatcc acgcccagcg
     7621 tgattcttgc ggtaccgaag cggcttaacc agtctgtcat tgcgcctgaa gcctgagaag
     7681 aggcccatcc acgcgccata tttgccgctt gctcgctgtt catatcttcg gcgagcagag
     7741 acccgatttg ctgtgaagta ctggctatct gttgctcaag gttgccactg ctattacccg
     7801 gtggcggggt taaattattt tcactaactt gtgccgggac atccagttca tcaccctggc
     7861 ggacattatc aaaacctcga gcaaacgtac gaaactggtt gagtttgcgt aactcagcca
     7921 ccgaaatacc gaagcgttcg gcaacgcttt gggccgattc cagcgctcca agggtatagg
     7981 gtaccgtatt ggcatttgca atggcgaatt gtgcaggaac tggttgttgg gttgcggcgt
     8041 ttaccacacc ttgtgctgcc gcagccatag ggaacgcaag ttgagttatc aggcagatac
     8101 ccgcagttaa gcggcgtagt ttaattccca tcccgcataa tatttgtcgg tcatttattt
     8161 cttctccact tctcttcttc gtagccatgc ttaccttccc tgaacgagct aacataaatg
     8221 cgccctttct agcacagcga aaaaatgtaa atcctgatag caatatgatt gaaaattaat
     8281 aacttttcgt taggcagttt tgagtgtgag ttgcaagagg ggagactact gaataactca
     8341 agttttataa tcgaggggaa aatggtgatg gcgttcatag caaaacgccc tcaaccataa
     8401 aggtcgaggg cgcttaagat gttaaaaacc cgctatccgt taaaaaacaa tgttcaacta
     8461 aggtcagtga cattgcgcta aaaaagcgaa tttttagaat ttggctcctc tgactggact
     8521 cgaaccagtg acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc
     8581 gtgtgaacgg ggcgcatatt acttagcggt accttgtctg tcaacactaa atttcataag
     8641 tcatttcaat tggttaatta atctgcaaag ttgttaatta atgaacattc gtcgccgaaa
     8701 acgagtctgt atcattagtg cgatgcagtc tctgcaaagg atcttgttga taaaattggc
     8761 agaaacgttg ccacaatgaa gggaaacgag gagcaaaaag ttctggggcg ctaaagaaat
     8821 attcagaaag tacggcaaaa cattcagcag gatcactggc agcataagca tcaatgctcg
     8881 ccgcattctc gccaaccaat tcgatttctt cctgaatgtt gttcattgca gcatgaagat
     8941 cgtgttccca gccagcaacc tcacgcaacg gaataaaggg aactccgctg gcgcgatcgc
     9001 cgttacgggt gtccagctta tgagcgactt catgaataat caggttaaaa ccagaagcat
     9061 caaaagaatc ttgtatatcc aaccagttca aaacgatagg cccttgctgc cagctctgac
     9121 ctgactgaac aatacgttgg ttatgcacca gaccgatatc gtcttcccat tcatcatcga
     9181 ccacaaatgg cgcaggataa attaagactt catgaaaacc atccagccat tccagtccta
     9241 actccagaac gggtaggcaa aatagaagtg ctatccggca gcttcttaat gaatccagtt
     9301 caaagccctg taaaggaaca agccgctttt gctgtaaaaa acgttcggca agagtgacta
     9361 atttgctttg ttcctgttcc gtcagacacg ttaaaagggg gatcgatagt gcttcctgcc
     9421 agggaagggc agtttgatgt gctgattctt gtactttcca gggccactta atcatcgttt
     9481 tgctcgcaaa ctcgtcactt gaacaaaatt gcacggacag ggactgttaa aatgccaaat
     9541 ttcctggcat catggcaacc atctgaacgg agagatgccg gagcggctga acggaccggt
     9601 ctcgaaaacc ggagtagggg caactctacc gggggttcaa atccccctct ctccgccact
     9661 ttatcaatga cttatctccc gacttcccgc cttgcttttc ctaaacagaa caatcgtaga
     9721 atattcttga agggttagat cgtcactgtt ttctgttcga tactgtgaca ttcagcactt
     9781 gattcctatg gatctgacag gaagctttcg agcgaaaatg tgcagttatt cagtcgtttt
     9841 cttatcgctc accattattc ttttagacat tcatcctatg aaactgccgc aaagttggtg
     9901 gtgggaactg aagttgcgta gagaaggggt caataattga gtcaaaaaag gaacgcggcc
     9961 gtcagattaa agccgcggga caaagtccat aatttccaag agttgttgaa tttcccag
//
LOCUS       DAPPNJ010000164         9858 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000164 DAPPNJ010000000
VERSION     DAPPNJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.165"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            205..1299
                     /gene="ddlA"
                     /locus_tag="RPM73_003456"
     CDS             205..1299
                     /gene="ddlA"
                     /locus_tag="RPM73_003456"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDX6521779.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1323..1535)
                     /locus_tag="RPM73_003457"
     CDS             complement(1323..1535)
                     /locus_tag="RPM73_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDX6521780.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1795..2103
                     /locus_tag="RPM73_003458"
     CDS             1795..2103
                     /locus_tag="RPM73_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDX6521781.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2162..3256)
                     /gene="yaiW"
                     /locus_tag="RPM73_003459"
     CDS             complement(2162..3256)
                     /gene="yaiW"
                     /locus_tag="RPM73_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDX6521782.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASSVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3269..4489)
                     /gene="sbmA"
                     /locus_tag="RPM73_003460"
     CDS             complement(3269..4489)
                     /gene="sbmA"
                     /locus_tag="RPM73_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDX6521783.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4841..5998
                     /gene="ampH"
                     /locus_tag="RPM73_003461"
     CDS             4841..5998
                     /gene="ampH"
                     /locus_tag="RPM73_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDX6521784.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5999..6622)
                     /gene="iprA"
                     /locus_tag="RPM73_003462"
     CDS             complement(5999..6622)
                     /gene="iprA"
                     /locus_tag="RPM73_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDX6521785.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6710..9598)
                     /locus_tag="RPM73_003463"
     CDS             complement(6710..9598)
                     /locus_tag="RPM73_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX6521786.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYNTFGYHDDAVTLFN
                     WGDNTKTDHDYLTYGGYVYDHAADGYFDTVFSGDTVNGVISTYYLNHDYGTDTANTLN
                     ITNSNIHGMITSDQIGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFN
                     DTYLDADGKTSKTDYDRLVTAALGTAVTLDVESNINISNNSHVAGITLVQNDLGNATY
                     NTEGHQWDNNIVVNNSTVTSGSLSEDEQSDRGHFGNSVEPSDYGNGASGADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKDSLIDTLAINSGSQVNVSADSSLTSDTITLNGSSSMEV
                     NGEVNTDHLIIDTFSTVNFGEDTASAWTSAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSVDNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2570 a         2419 c         2337 g         2532 t
ORIGIN      
        1 atggccattt aagccaggaa taaattagct tcccgttcat catttttgtg atcttgcgca
       61 catctttgag ataacgcact ttcttcacca gtacgttgca cgcttcgtca gcagaagatt
      121 gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg
      181 tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt
      241 aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat
      301 aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc
      361 gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg
      421 gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac
      481 ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa
      541 gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt
      601 ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg cgatgccggg
      661 ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc
      721 gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca gggctcttct
      781 gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga tctggcgttc
      841 gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat cgaatgcgca
      901 gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat
      961 ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca
     1021 gccattgcac cagagatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg
     1081 ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg
     1141 atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg
     1201 caagccagcg gtctcggtta caccgatctg atcacacgtt tgattgagct ggcgctggag
     1261 cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc ccgataatat
     1321 tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc cgccagccag
     1381 aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt tgcttcaaat
     1441 cccagtaacc ccaccacgcc attaagaatg aatataaggc caatggcgaa cgcatagtag
     1501 tgccagtcac ggcggatttt tacaggcaga ttcatggcag ctccgttaac gaaaaacaac
     1561 atccttgcat attctgtaac aaccgtctgt ggtgtagaca acattttcgt aattgttaat
     1621 aaatttaact taaggttgcc attttgtgac tcacaacgaa gattcacgct tcgggaatat
     1681 tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg tcagacttgc
     1741 cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt ttttatggct
     1801 gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct
     1861 ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc gcaattgctg
     1921 aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt
     1981 cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat cccgttttta
     2041 gtaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca tcgccgccag
     2101 taagatttac gttgccagat acggcctaaa acgccttatc tggcatgaca ttcctgtttg
     2161 atcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag
     2221 tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt tcgcggggta
     2281 gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc gccgtctcct
     2341 caaaagagaa gctgtcacct ttctctaact gacggcgaat ttcgctgtcg ctcatcccca
     2401 gttttgccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta ctgtcgtaac
     2461 gaattaaatc accgtccagt gccagcttca cgctgctggc cttactgact gcgttctgaa
     2521 acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaagcgg tatatcggtg
     2581 cgctatagct ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc
     2641 gactgaagac ttcctgacga actgtaccgt ccattttcca cggataccct ttggtatgct
     2701 gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcacagga ttaagcgaac
     2761 caaacagcgt ctgccccatt ggtaccatgc tgatcagatc gtcaaaaatc gcgctcaatt
     2821 gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt ggcgatttga
     2881 tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa
     2941 tctcttgcca ggcgatcttg cttaagcccg gaacggccgg atctgcctga taatttgact
     3001 cctgctgcgc caccgccagc accgagcaga cgttttccag cgtcggtgcc agcccctgac
     3061 tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca
     3121 tcttctgccg cacgacgctc gctacatcta tcgctttttc gccctttttc aacggttgcg
     3181 gagcctgact actacacccc gctaaaacca gaaccgccaa caatgacagc gaagatagat
     3241 gattgacgcg cgacattact tctccttttt agctcaaggt atgggttact tcctgaattt
     3301 tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt gtagatagac atcaactcaa
     3361 ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt
     3421 tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca
     3481 agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg ttgaaataca
     3541 tatagtgaaa atagaggcgg aaatagtttt tccgcacggc gctaaacagc tcgcgtaccg
     3601 taggcggcgt cgcacgcgtg gcatcgtctt caccataaac cagctcttta cggtaggcgg
     3661 cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc actgccagca
     3721 atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac ggaatgtgcc
     3781 cgataatcgg cagctccggc acatgcgtgg agagcgttac cagcaccggc aggaaggcga
     3841 tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt gaagcaaaac
     3901 gcatggtgtc ttcctgcaca cgctgtgcgg ccccttcgat atgacgcagt tgttgccagt
     3961 tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga ctgacaaaga
     4021 agttgttcag cacactgatc accacagcga tcagcgcaat ccccagaaag acgcccactt
     4081 cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt tgaatcagat
     4141 catagaacgg tgcataccag gcgttgacgg cgaccccgac ttccaccaaa aaccaggtga
     4201 cgaagatgat cagtgcagta ccgagtattg accagtattg ccaacggtgc gggctgtaga
     4261 taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga
     4321 aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg ccggtgatac
     4381 gcgccaccca gtcaccccca ccggcttgcc agaagataac ggcaatcaat gcccaaacaa
     4441 aggccgagag aaaaaacgct cccggctttg ggaaaaaaga cttaaacata tgcactcctg
     4501 ctaacttctt atcgttttaa caactgctgt gttttaaccg catgaccgct acaaagcgtc
     4561 agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc agcctgacca
     4621 taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg gattaacacg
     4681 cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta cgcgccgtta
     4741 cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg agtagtataa
     4801 atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta gtctgctttt
     4861 ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc cgatcactga
     4921 accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt acggcagcgg
     4981 tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc gtagttatgg
     5041 cgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc gtattgcttc
     5101 cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg ggaccgtaaa
     5161 gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccga cctacaatgg
     5221 gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc gtgaacagcc
     5281 cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgaacaac gctggaaata
     5341 cctttctacg gcgaagctaa aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc
     5401 gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata cccagttgtt
     5461 tgaagagcag attacccgtc cattggggat gaaagacacc acctacaccc cctcaccgga
     5521 tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata acacgctggc
     5581 ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct ggatgcagca
     5641 gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc agacgttgat
     5701 ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca aagcggatgc
     5761 gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgggga ttattcagaa
     5821 gacaggcggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga agaatattgg
     5881 cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat
     5941 caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg catcctgatt
     6001 aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt tcgatatagc
     6061 cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg cgtgtacgtt
     6121 gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag tcaatcattg
     6181 ataataatgt tgcgcggatt tgttcgtagg aattatgccc aatgagctgc acgtcgcgta
     6241 attccagaat tctattttgc caggctaacc agtaaaaagc gtctcgccag agttgatttt
     6301 gttcaataag cgtaatggtt tgtttggctg gtagatgata tcccgtacaa tttccttctg
     6361 atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc ataatataag
     6421 gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata acgccctcta
     6481 aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc ttttcattat
     6541 taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca aattcctgaa
     6601 gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc aaatccttga
     6661 cgggcattat ttagattaaa ttaccagaat ttcttcggag tgaagaatat taccaggtat
     6721 atttaacacc cacgttcgcg gaccaatctt gatctacgtc accaccaccg aggtagttag
     6781 catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta
     6841 ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta tcgttgttag
     6901 agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctggtct tcgctgtagg
     6961 tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa gactgaccgt
     7021 caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga ccagaaatgc
     7081 tgccgtaagg agtcacataa ccagcatcac ccagtttgaa gtcgtaaccg gctttcaagc
     7141 cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg ctcatggttg
     7201 cagacaggtc gttgttgaag tgagagtagc tcaagctacc atcaacaaag acgttgttcg
     7261 cgaagtgagc agaagagtag atgtaggcag tctggctgtc ttgatctacc tgaccagaac
     7321 ggtcattcat gtcaccttta gcgaagcctg cagccgcacc gacgatccac ttagcgttgt
     7381 taccgtcaat tttggtatca acaccgacca tgatgccgtt aacatcctga tcatagttga
     7441 tggtgccgtt gtcgccgttg aagttaccac cgaagtagct tacccatgcg ccgccgttat
     7501 cagccaggcc atgacgagag ttggtcagac gagtaccaac ggtgtcttgt tccaggttcc
     7561 agatattggt gttcgcagaa gggatgctca gcgccatgtt agcgtagtcg gtcagctcca
     7621 tctgttgcag aacaacggtg ttaccgcgct gttcagcctg ataggtgtat gcacccaggt
     7681 cagctttgtt agcagcagag aaggtcgcgt tggttttggt atcatcgata tagatgagct
     7741 gcttacctgc gtaatcagca atcgcgccag caccagtgga gttgtcgata cgaactttgt
     7801 agttaccggt tgcagctaca acgttgtcaa cagagctgtt gtcaacttcg tcgccattat
     7861 taatgtcgtt gttaccatta acggtcaagt gaccatcaga gttcatcgca ataacaccat
     7921 aaccatagtt agctttgctg gtgtcgttgg tacgatcgtt gacgaggtct gcattcagga
     7981 tgtagtcatt actatgaatg tcaaacacac ctgcgtaaac attaccgttg ttatctttaa
     8041 cgttgccact ggtcagttcc agagtatcag ttgcaaatac gctggagatg tcattcatgt
     8101 tggtgtttac atccagcaca ccaccgttag taacggtaat ggtgttggcg tacagcggtg
     8161 cagatgtcca agctgaagca gtatcttcac cgaagttaac agtagagaag gtatcaatga
     8221 tcaggtgatc agtgttaact tcaccgttaa cttccattga agaactaccg ttcagagtga
     8281 tggtgtcgga agtcagggaa gaatctgcac tcacgttaac ctgagaaccg ctgttgatag
     8341 ccagggtatc gatcagagaa tctttcgtgg tttcccaatc agaaccattg ttcagaacaa
     8401 catcgaatac gccactctgg aatgcgttag cgttatcaac acgattccag ccatcttcag
     8461 agtaagaagc gtacgggttc aggctgtttg gcgcaacgtc gtagaaggct tcgtcagcaa
     8521 caacaacgtc ataatcaata ttcgctgaac caacccattt ggtgttgttc agagtcaggt
     8581 tcaggtgatc gacgttctga tcgtcagtag cccaaccgcc atgagtgtaa gcagtggtag
     8641 agttgtcaat caggtgaccc gtcgcgtcaa agttagcgtt ccaggtactg tcaaatacaa
     8701 cgtcgccaag caactgagaa ttgttgaatg taacgttgtt gaccatacga taatcagaag
     8761 tgtcatcatc tatgaaggct aatgcaacat catcagcacc tgatgcacca ttaccatagt
     8821 cgcttggctc aacagagttg ccaaaatgac cacgatctga ttgttcgtct tctgagagcg
     8881 aacctgaagt tacagtggaa ttattaacaa cgatattgtt gtcccactga tgaccttcag
     8941 tattgtaagt cgcattacct aaatcgtttt gaaccagagt aatacctgca acatgggagt
     9001 tgttgctgat attgatgtta ctttcaacat ccagagttac agcggtgcca agagcagctg
     9061 ttaccagacg atcataatct gttttagacg ttttaccgtc tgcatccaga taagtatcat
     9121 tgaagtagaa tgcatcaaaa tcatcgtcaa ttgttgagtt cgcgatgttc agagtgaaaa
     9181 tatcgccatc tacccaatca tgaccagtat aatcgctacc gttggtccag acgtaatcac
     9241 cgtatccgat ctgatcggaa gtaatcatac cgtgaatatt tgagttcgta atattcagag
     9301 tattagcggt atctgttcca tagtcgtgat tcagataata agtagaaatt acaccattaa
     9361 cggtatcgcc actaaataca gtatcaaaat aaccatcagc cgcatggtcg tatacatagc
     9421 caccatacgt caaatagtcg tgatcagttt tagtattgtc gccccaattg aatagagtga
     9481 ctgcgtcatc gtgatacccg aatgtattat aagtagttgc attagcctga gaggtgatag
     9541 ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag gagtgcattt
     9601 gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca tagaaaacat
     9661 attatgtttc gcgctaatga ttatgctgcg ttacagacga atggttcaat tattctttcc
     9721 cctaagtccg aatgtggaca tatataacct atggagaaaa acacgcatca aattgataaa
     9781 agggcgatat ttcaacatgt tgcagataat gttatttttg atacatacgc gttataaata
     9841 tcaaaaacac aaaccaga
//
LOCUS       DAPPNJ010000165         9790 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000165 DAPPNJ010000000
VERSION     DAPPNJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.166"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            127..558
                     /gene="osmC"
                     /locus_tag="RPM73_003464"
     CDS             127..558
                     /gene="osmC"
                     /locus_tag="RPM73_003464"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDX6521787.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDTSTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(614..1540)
                     /locus_tag="RPM73_003465"
     CDS             complement(614..1540)
                     /locus_tag="RPM73_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6521788.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQDNHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQILELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(1533..2519)
                     /locus_tag="RPM73_003466"
     CDS             complement(1533..2519)
                     /locus_tag="RPM73_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX6521789.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2516..3412)
                     /locus_tag="RPM73_003467"
     CDS             complement(2516..3412)
                     /locus_tag="RPM73_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDX6521790.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3409..4431)
                     /locus_tag="RPM73_003468"
     CDS             complement(3409..4431)
                     /locus_tag="RPM73_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521791.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     DSDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4433..5983)
                     /locus_tag="RPM73_003469"
     CDS             complement(4433..5983)
                     /locus_tag="RPM73_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX6521792.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRHLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5997..6578)
                     /gene="ddpX"
                     /locus_tag="RPM73_003470"
     CDS             complement(5997..6578)
                     /gene="ddpX"
                     /locus_tag="RPM73_003470"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDX6521793.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(6836..9235)
                     /gene="dosP"
                     /locus_tag="RPM73_003471"
                     /gene_synonym="pdeO"
     CDS             complement(6836..9235)
                     /gene="dosP"
                     /locus_tag="RPM73_003471"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDX6521794.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGCQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9260..>9790)
                     /gene="dosC"
                     /locus_tag="RPM73_003472"
     CDS             complement(9260..>9790)
                     /gene="dosC"
                     /locus_tag="RPM73_003472"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDX6521795.1"
                     /translation="ELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLS
                     VLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEAS
                     ENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYI
                     AKRRGRNRVELWKASL"
BASE COUNT         2402 a         2557 c         2483 g         2348 t
ORIGIN      
        1 gtggaaattt aatttaagtg cagaagtaat atttctgccg gatttttatt cggaatatcc
       61 tgcttatcct cgtgctgttt ctcacgtagt ctataatttc ctttttaagc ccacaggaga
      121 gcaacaatga caatccataa gaaaggtcag gcacactggg aaggcgatat caaacgcggg
      181 aagggaacag tatccaccga gagtggtgtg ctgaaccaac agccgtatgg atttaacacg
      241 cgttttgaag gcgaaaaagg aactaaccct gaagaactga ttggcgcagc gcatgccgca
      301 tgtttctcaa tggcgctttc attaatgctg ggggaagcgg gatttacgcc aacatcgatt
      361 gataccaccg ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc
      421 gcactgaaga gtgaagttgc ggtgccgggt attgatacct ctacctttga cggcatcatc
      481 cagaaagcaa aagcaggatg cccggtttct caggtactga aagcggaaat tacgcttgat
      541 taccagttga aatcgtaaag cattgccgga tgacgcgtca ggcgcgtgaa tgcctgatgc
      601 gttgttagca tctctacagc gcccgccagc accggatctc acgtccgtcc tcccttattg
      661 ttaatgattg ccggacttca catccgtggg ttgccagagg gcaacgttcg tagaaaaaac
      721 agccttgcgg caacgtgcgg tttcccggca gatccgtttt acgtaatgcc cattcttcct
      781 ccagcggttt atcaatggcg gggagggaat ccagcaataa tcgggtatat ggatgtgccg
      841 gagcagtcaa cacctgttgc gcttccccta gttccaaaat ctgcccgaga tacatcaccg
      901 ccacccgatc gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataagtca
      961 gcccgtgatt gtcctgtagc gttaccagta aattgaggat ctgcgcctgc acggagatat
     1021 ccagcgcaga ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg
     1081 caatggcgat acgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact
     1141 ccggacgaat acccacctgc acagccagct cctctgccaa cgctcgccgc tgttgttcac
     1201 tactacgctt agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat
     1261 tgagcgaaga gagcgggtcc tgaaacacca tctgcataat gcgttgtgag ccagaacgga
     1321 tgtactgccc gtggctcggt tgcagcatgc ccattaaaag ctgtgcgagg gtgcttttgc
     1381 cgcagcctga ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac
     1441 cattaatggc atgaacatgt tccgtagttt taccaagcca gtttttacgg gccgggaaat
     1501 tgatatggac gtcgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga
     1561 taccaacagg cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca
     1621 cactgtgcac ctgccgcata gcaacgatcg cgaaaagcgc agccttcagg caaatgggtg
     1681 aggtttggta ccgtccctgg aatggcgggt aatggctggc gtggtactcc atgttctggt
     1741 gcgcattgca gcaaaccaat ggtatacgga tgccggggat gatggataat gtctgccgtc
     1801 acgccgcttt caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac
     1861 accacgacca tatcatggct gatgaacagt accgcagttc cactggcgcg ggctttatgt
     1921 ttaagcaaac gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg
     1981 gcaataatca attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata
     2041 ccacctgaaa gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc
     2101 tcttccagca gggcaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg
     2161 cggatcacgt ccatcatctg aagacctatt cgaagtgtcg gattgagggc ggtcatcggt
     2221 tcctgaaaga tcatcgccac tcgtgcgccg cgccattgac gaagctgttt ttcccgagcg
     2281 ttaagaacat cgtctcccag tagtgaaatc tgcccccgat gtacgcaata actgcccgtc
     2341 ggtagcagac gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc
     2401 agaccgacaa tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca
     2461 ccgttaaaac cggggaaact caaatgcagc tgttgaatgt ccagaacggg ttgggtcatg
     2521 actgctttcc tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa
     2581 acccaacggc ggtaagcaaa atcgctgccc ccggaaatgc gcaataccac cactgatcga
     2641 gtacatagtt gcgaccattt gctaccatcg cgccccattc agcactcggt tgttgagcac
     2701 ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg
     2761 cctgcacgat cagcggcggc agggagttac gtaaaatatg ccagctgatc agatgccaac
     2821 gcgacgcacc aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt
     2881 gcccccgcgc caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc agcatggcgt
     2941 taaataaact cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg
     3001 acagcataat gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg
     3061 atagacatcc gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga
     3121 gaattgattg ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg
     3181 tgccaaacca gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg
     3241 gatcatgcgt cgcaagccac ggtgaaaaaa tcatcatcaa tagcattaat acaatgatga
     3301 ctgcgccggt cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa
     3361 atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc
     3421 tccacgtccg atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag
     3481 cacataagca aatgaaacca cgacggcaaa tcccatcacc gcagggaagt cgagcgcctg
     3541 tattgatgtc actacccatg cccccattcc gggccaggca aaaacggttt cggtgagcac
     3601 tgcgccatac aacaaatcgc ccagcgccaa acccaatacg gtaatcgatg ggatcaacgc
     3661 attgggtagc gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt
     3721 acgaatgtag tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat
     3781 tcccaggtga acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa
     3841 gaagacttca ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt
     3901 tggtggatcc agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata
     3961 aaacagcaca atgacgccca gcccaagcca gaacgcaggt gtggagattc cggtaatggc
     4021 cattattcgc accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag
     4081 cgggatgcca atcaggagtg ccagcagcag ggcaccaaaa gccagctcca gcgtcgccgg
     4141 gaaaaatata cgcaactctt ccagcaccgg acgcccggta cgaatggatg ttcccaggtc
     4201 accatgaaac aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc
     4261 cagttgctgg cgaatatttt ccacgatagc atcgctgtcg cggtcacccg ccagtaaccg
     4321 cgccggatcg ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccac
     4381 cacgagcacc agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac
     4441 tcatggtatt gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct
     4501 ctttgttcat cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa
     4561 tgacgatttt ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa
     4621 gcgcattgcg cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca
     4681 gacctttttt gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg
     4741 gactccagtt gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg
     4801 cgttcgccag cttttccagc ttcacattga tgcccagctt gttgagactg gattgtgtcg
     4861 ccaaagcaat aggctcccag ttcggatcgt tatcggagta gagaaacgtc aggctggtgg
     4921 gtttgctcgt cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca
     4981 ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt
     5041 taccactcag aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac
     5101 gcagatccgc ctgattaaga ggcgctttgc tgttattcag atacagatag gtaacgcgca
     5161 gtgacggata ctctgccaca ttgactttat tttcctgctt cagggcgttg agttgatcca
     5221 ccggcagcgc atcggcaatg tcaatgtcgc cacgggagag ctgcaggtga cgggaggcac
     5281 tttcaccgat aatttttacc gatactcgct taaagttcgg tttattgccg gggtaatgcg
     5341 gatttggcac cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg
     5401 aaccggcggt attttgcgcg aggaagccgc gagcatcatc cgctgcatgt tcctttaaga
     5461 ccgccggatt gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg
     5521 gttggctaag ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg
     5581 gaaatgcttc tgctggcccc tggccgattt ttagtagccg ctcaaaagaa agttttactg
     5641 cttcggcagt gacaggtgtg ccatcggcaa atttagcatt atctttcagg gtgaacgtcc
     5701 actctttttg atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg
     5761 agcctttatc accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcactg
     5821 tccagtcgtt attatctatt gttaccgccg ggtcgagggt ttgtggatcg gcggccttac
     5881 caatcaccag catatctttc ggtacggcgg cgtgcgcaac cgggaaattt gtggcaagga
     5941 cgagcgcgag caatgtggga cgaaacgata tcgatctctt catgacagtt tcctgatcaa
     6001 ctgacgtgct gtgtgccagg ggatataaaa caagagaatt gatcagcgag cagagggtaa
     6061 ctcgctgcct ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca
     6121 ccagtcatta tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg
     6181 tgataggcat gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc
     6241 ccgtgctcat cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc
     6301 gtcacatcaa caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt
     6361 tgtgggcgat acgcatcgta aatcaccagt tgtaagcctg acagctgggc gatgctgata
     6421 cttttcgcca gcgcggtaat cgcatccttg tgtaacagac aacgcgcttg ctgataaata
     6481 gctttacctg tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag
     6541 attacggcta aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa
     6601 aaattgctgc gctttccgct tatgcagatc tcatgccatg ccgggagaag cgccagaatg
     6661 ctggcttaaa gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt
     6721 ggtgcgtgag gggaagttaa ggaaggaatc tcccggaatc gtagctgaaa tcacagtatt
     6781 taagtgacag tgtcacctta aaggaaaacc cgcgagtgcg ggcgagagga atttgtcaga
     6841 ttttcagcgg taacacgctg ctcatccagc ctggaatttc ttcggcggga agggggcggg
     6901 aaaagaaata tccctgaata acgcgacagt ggatcttacg tagcatctca aattgctctt
     6961 tggtttcgac cccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg
     7021 cttcaagtaa ggccaggatg cgtttttccg tcagacaacg atcgacaaaa cttttgtcaa
     7081 ttttgatttc cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa
     7141 aatcatctac cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt
     7201 cggtatcgtg ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa
     7261 taccccaggc gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca
     7321 gtgccgacaa gttaacggaa agcgccggga tatgaatatt ctggctacgc cattctgcta
     7381 actgacggca agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt
     7441 ctgcgagagg aataaaccgt gaagggggca catgaccatg ctggggatcg tgccagcgcg
     7501 caagggcttc gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacaa
     7561 gtttcagttg gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt
     7621 ttaccatttc gttcatcgcc gggctgaaga actggcagcc gttaccgcca ttcttgcgaa
     7681 tataatccat tgcattgtga gcagtggaga gcaggtaatc gcggttttta cccacgtcgt
     7741 agctgatgcc aatactcaag gtaagcggga atggtttatc gtcaatcatt atcggcttgc
     7801 tgaccacatt ccgtagctca tcggcgatct gggtaatgtt actgacgtcg ttttccaggc
     7861 tcacgaggac aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttt
     7921 cacgaaagcg attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat
     7981 caatcacatc ctgaatatgg tcaacaccga tgagatacac cacgggagag acggctttgt
     8041 cgaccaggtc atcgaggtaa ttgtgcaggt tattgcgatt tggcagaccg gtcatcggat
     8101 caaattggat gagttgttca atatgctggc ggcttttttc ctgttccagc gccagcgcgg
     8161 ccatatgctg actgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg
     8221 aggttttaat ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc
     8281 agctttgcgc attttgaact tctgcaccgt gcgaagatga cgcccagtgt atcggcatcc
     8341 cgttgcgcag tgcgaacagc gaaacatgcg attcgttgag aacagattcg atgttacgac
     8401 aaatgatttc ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc
     8461 cttcaagctg gcggatttgc cgttcttcgg tgatatccga gaaagtcatt accaggttct
     8521 gcagatgcgc gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac
     8581 cggtgcgcgt caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt
     8641 gtaaacgaat gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca
     8701 taccgctggc ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca
     8761 caatatggcg ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa
     8821 tcaattgtcg agtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca
     8881 ggtaataaac tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac
     8941 tgccgtcttt tttctccagc tgcagctccc gactcatccc ctcgacacgc gctttaccgc
     9001 cttcacggtt atgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca
     9061 tatcaatgtt attgccaatg acttcttcac gtttgtaccc ccagagcttc tctgcggcgg
     9121 ggttgaaaaa catcacttca tcattttcgt taattaacac cgcacccatc atattttgct
     9181 caagggcggg gaaaaaaatg ccatcggcgg cattatccgc atcggttagc ttcatgatta
     9241 cctctgcatc ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc
     9301 gtcttttggc gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat
     9361 gaccattaaa catggcggca ccgatggaaa gtgagagggc aatatcctcg ccgtttgcgg
     9421 ctttcagttt ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt
     9481 tttcagaagc ttcagtcaaa acaatgataa attcatcgcc cccgtagcgg aaaacataat
     9541 cactactgcg gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcgccag
     9601 tgttatggcc ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg
     9661 acagcggtgt accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggcagga
     9721 aacggcggtt aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt
     9781 caaacaattc
//
LOCUS       DAPPNJ010000166         9726 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000166 DAPPNJ010000000
VERSION     DAPPNJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.167"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            86..301
                     /gene="bdm"
                     /locus_tag="RPM73_003473"
     CDS             86..301
                     /gene="bdm"
                     /locus_tag="RPM73_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDX6521796.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            403..540
                     /gene="sra"
                     /locus_tag="RPM73_003474"
     CDS             403..540
                     /gene="sra"
                     /locus_tag="RPM73_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDX6521797.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            697..2394
                     /gene="maeA"
                     /locus_tag="RPM73_003475"
     CDS             697..2394
                     /gene="maeA"
                     /locus_tag="RPM73_003475"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDX6521798.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2528..3538
                     /gene="adhP"
                     /locus_tag="RPM73_003476"
     CDS             2528..3538
                     /gene="adhP"
                     /locus_tag="RPM73_003476"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDX6521799.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3685..3969
                     /locus_tag="RPM73_003477"
     CDS             3685..3969
                     /locus_tag="RPM73_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDX6521800.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4020..4673)
                     /gene="fdnI"
                     /locus_tag="RPM73_003478"
     CDS             complement(4020..4673)
                     /gene="fdnI"
                     /locus_tag="RPM73_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDX6521801.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4666..5550)
                     /gene="fdnH"
                     /locus_tag="RPM73_003479"
     CDS             complement(4666..5550)
                     /gene="fdnH"
                     /locus_tag="RPM73_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDX6521802.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5563..8610)
                     /gene="fdnG"
                     /locus_tag="RPM73_003480"
     CDS             complement(5563..8610)
                     /gene="fdnG"
                     /locus_tag="RPM73_003480"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8023..8025),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDX6521803.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8842..9723
                     /gene="yddG"
                     /locus_tag="RPM73_003481"
     CDS             8842..9723
                     /gene="yddG"
                     /locus_tag="RPM73_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDX6521804.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     WLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2328 a         2560 c         2474 g         2364 t
ORIGIN      
        1 cggcaaatat ttgccatgct gaatgtgctt tataaatctg cgatccgtag cagacaccat
       61 aaatacacag acacggagaa tcactatgtt tacttattat caggcagaaa attcaacagc
      121 agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcacg gtgttgtcac
      181 tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata acgacatcct
      241 cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc ctacgcttta
      301 aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc ttcgctacgg
      361 cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag
      421 gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat agttaccgaa
      481 ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc tgaaaagtaa
      541 ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat ggtgtttttg
      601 tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga cgataaaagc
      661 cccccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag
      721 cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt gttgaataaa
      781 ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt actgccggaa
      841 gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca gggattcaaa
      901 accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga aaccctcttc
      961 taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac cccaaccgtc
     1021 ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt gtttatctct
     1081 taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccaaacca taatatcaaa
     1141 gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg catcggcggg
     1201 atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat cagcccggcg
     1261 tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagttgct taacgatccg
     1321 ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga attcgttgat
     1381 gaatttatcc aggctgtgaa acaacgctgg ccggacgtgc tgttgcagtt tgaagacttt
     1441 gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg ttcctttaac
     1501 gatgacattc agggcactgc ggcggtaaca gtcggcaccc tgatcgcagc cagccgcgcg
     1561 gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc
     1621 ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga ggaagcagcg
     1681 cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat gccgaacctg
     1741 ctacctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg ggataccgac
     1801 agcgatgtgt tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat tctgattggc
     1861 gtctcaggac agaccgggct gtttacggaa gagatcattc gtgagatgca taaacactgt
     1921 ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag
     1981 gacattatcg cctggaccga aggtaacgcg ctggtcgcca ctggcagccc gtttaatcca
     2041 gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt tattttccca
     2101 ggcatcgggc tgggtgttat tgcttccggc gcgtcacgta tcaccgatga gatgctgatg
     2161 tcagcaagtg aaacgcttgc tcagtattcg ccgctggtgc tgaacggcga aggtctggta
     2221 ctaccggaac tgaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa
     2281 atggcgcagc agcaaggcgt ggcggtgaaa acgtctgccg aagctctgca acaggccatt
     2341 gatgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat ctaagcctgc
     2401 gcccggtagt gaaggctacc gggctatttc tctctccctt tttcagatct catccatact
     2461 gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca tccgaaaagg
     2521 aggaactatg aaggctgcag ttgttacgaa ggatcatcac gttgacgtta cggataaaac
     2581 actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca
     2641 taccgatctt catgttaaga atggcgattt tggtgacaaa actggcgtaa ttctgggcca
     2701 tgaaggcatc ggtgtggtgg cagaagttgg cccaggtgtc acctcattaa aaccaggcga
     2761 tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact gtaatagtgg
     2821 taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg gcgggatggc
     2881 ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc
     2941 ggcggccagc agcattacct gtgcgggggt caccacctac aaagccgtta aactgtcaaa
     3001 aattcgtcca gggcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgctct
     3061 gcaatacgcg aagaatgtct ttaacgcgaa agtgatcgcc attgatgtca atgatgagca
     3121 gttaaaactg gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc
     3181 cgccaaaatt gtgcaggaga aaaccggtgg cgctcacgct gcggtggtaa cagcagttgc
     3241 taaagctgcg tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt
     3301 cggtctgccg ccggagtcta tgagcctgga tatcccacgc cttgtgctgg atggcattga
     3361 agtggtcggt tcgctggtcg gcacgcgcca ggatctaacc gaagccttcc agtttgccgc
     3421 cgaaggcaaa gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt
     3481 taccgagatg gaagaaggca aaatccgtgg ccgtatggtg attgatttcc gccgttaaga
     3541 ggcctttgct gcgagtgcca gtcctggtgt cgcagcatcg cactctctcc gacattaaat
     3601 ggctaaatgg attattgttc cttcctacgc aacaataatg atagtgttcc tgatgaagga
     3661 acactactga gagtaaagac actgatgaaa atggccaatc atccccgccc gggggacatt
     3721 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3781 attgctccct caacggcaag ccgattgctg accggaaaag cagctttgac accagaaatg
     3841 gcgattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3901 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgactacg ccgtctggtt
     3961 acgcaataaa aaaatggcgc ttcttaaagc gccattttca gcttaaagtt caaaagagat
     4021 tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgt
     4081 ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac
     4141 cctttcaccc aaaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc
     4201 gcagccgcgt ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag
     4261 tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac
     4321 cagaacatca tcttttgccc ggcgttgtac ttaccgacat ccgccacttt atgctcattg
     4381 cctttcaata cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg
     4441 tgatgcacaa aacgcacaaa cataaacatc agcgcgacga aaatcgcaat gccgaagaac
     4501 gggtgcaaaa tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc
     4561 gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag
     4621 tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc
     4681 ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc
     4741 ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct
     4801 taccgaggtg tcgatcttcg gatctttcgg cagaccgtga tacagctccg gctgatcggc
     4861 gtgatgcagc acgtacataa cgtgcgtacc accgacccct tccgggttgt agacgccagc
     4921 atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct ccagcatctc
     4981 cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg
     5041 gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt
     5101 gaggcgcgga atattaaatg gacacccggc aatgcagtaa ccacagccga tgcagttttc
     5161 cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc
     5221 cttcaggcag cccggatctt cacagtgcat acagccgtct ttacggatca gccactccag
     5281 cttgccgttc tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc
     5341 ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc
     5401 cgaacacgcc acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagtttcgc
     5461 gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct
     5521 tttgataatg tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt
     5581 gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag
     5641 agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc
     5701 caccgtttca acctgctgac cgtttacatt cagcggtttc agacggcgcg ttaccaccgc
     5761 caccgcgcgg ataaagccac gcttgcttga gacagtgaca cgatcgccat tattaatgcc
     5821 tttcgccgcc gccagcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt
     5881 gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac
     5941 atacgggaac tgctcttttt tacccatccg cagcgcgtct tgttcataca gacgaacaac
     6001 cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt caattggctc
     6061 gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc
     6121 ttccggctgc atgataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtccgg
     6181 aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca
     6241 cggtttaccg ttgatatccg ccgaagcacg gttgtacagc acgcgacggt tgagcggcca
     6301 cgcccaggcc catcccagcg tattccccag accggacggg tcggagttat cgcggttagc
     6361 catctggttg ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt
     6421 accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac
     6481 gccatttgcg tcatagagat cttccagcgc atagccgttg ttctcttttg ccacttcgtc
     6541 agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc
     6601 tttaccacct tcggcctggt acagctcgcg cagatgatgg tagatacccg ccagaatttc
     6661 gccgtcgtta cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact gcaaccagcg
     6721 accggagtta gcgatagaac catcttcttc ggcaaagcag gtcgaaggca gacggaatac
     6781 ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt
     6841 agaagtttca gtcaccagcg gatcgataac caccatgtac ttcagcttgc tcaggcagct
     6901 caccactttg tttttgtccg ggaaggacgc aaccgggtta aagccctggc agaaataacc
     6961 ggtgactttg ccttcatcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca
     7021 cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga
     7081 tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct gatcagccag
     7141 cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg
     7201 cagcgtcaga taacctggca ggctggtaga gagcaggcct aagtcagtca gaccctgaat
     7261 gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag
     7321 caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc
     7381 cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca
     7441 cactttcagg aagtcggctt ttggcgtacc acagatgttt tcaacaacgt ccggcgtgta
     7501 gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc
     7561 gcgtttcgca tagccgtttt catcgaactg atagttccag gacgatttat cgtattgccg
     7621 tttttcagcg tcgtagccgc tgaacagacc gtcttcgaaa gcaaaatcat cacgcaccag
     7681 caggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag
     7741 gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat
     7801 atccgccaca gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg ttgcatcgtt
     7861 gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc
     7921 gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg tcatcgcacc
     7981 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt
     8041 gtctaccgcc agcatcccga gggagcgggc aaatttctgt gtcagcatcc cggtttcgtt
     8101 gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttactg ttacgccctg
     8161 ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagctttg caatacggga
     8221 gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatattccgg
     8281 gtagcgcaga cggttttcgc tgttgacgta atccagcaaa ccggcccctt ttgggcacag
     8341 cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc
     8401 gtttttcgcg ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt
     8461 gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc
     8521 ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat
     8581 tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg
     8641 cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg
     8701 tttgccacaa aataatccgc ctgcgcaaat tgacctacct caatagcggt agaaaaacgc
     8761 accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc
     8821 aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg
     8881 atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggcccg
     8941 gtcggcggcg cagctgctat ctattcatta agctggctgc tgttaatctt cacggttgga
     9001 tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc
     9061 agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt
     9121 gaagtgggta tggtgaacta tctatggccc agcctgacaa ttctctttgc cattctgttt
     9181 aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc
     9241 tgctgggtgt taggcggtga taatgggtta cattatgatg aaatcatcaa taatattacc
     9301 accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc
     9361 acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg
     9421 ggagcaagtc tgtgggttta ctattttctc acgccacaac cagaaatggt atttagcacg
     9481 cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta tgctgcatgg
     9541 aatgtcggta tattgcatgg taacgtcacc ataatggcgg taggttcgta ttttacgccc
     9601 gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg
     9661 caaggcgcgc taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt
     9721 taaaat
//
LOCUS       DAPPNJ010000167         9615 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000167 DAPPNJ010000000
VERSION     DAPPNJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.168"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            395..2026
                     /gene="oppA"
                     /locus_tag="RPM73_003482"
     CDS             395..2026
                     /gene="oppA"
                     /locus_tag="RPM73_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDX6521805.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2112..3032
                     /gene="oppB"
                     /locus_tag="RPM73_003483"
     CDS             2112..3032
                     /gene="oppB"
                     /locus_tag="RPM73_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDX6521806.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3047..3955
                     /gene="oppC"
                     /locus_tag="RPM73_003484"
     CDS             3047..3955
                     /gene="oppC"
                     /locus_tag="RPM73_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDX6521807.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3967..4980
                     /gene="oppD"
                     /locus_tag="RPM73_003485"
     CDS             3967..4980
                     /gene="oppD"
                     /locus_tag="RPM73_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDX6521808.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4977..5981
                     /gene="oppF"
                     /locus_tag="RPM73_003486"
     CDS             4977..5981
                     /gene="oppF"
                     /locus_tag="RPM73_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDX6521809.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6034..6363)
                     /locus_tag="RPM73_003487"
     CDS             complement(6034..6363)
                     /locus_tag="RPM73_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDX6521810.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6398..7858)
                     /gene="cls"
                     /locus_tag="RPM73_003488"
     CDS             complement(6398..7858)
                     /gene="cls"
                     /locus_tag="RPM73_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDX6521811.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8001..8174
                     /locus_tag="RPM73_003489"
     CDS             8001..8174
                     /locus_tag="RPM73_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDX6521812.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8229..9482)
                     /gene="kch"
                     /locus_tag="RPM73_003490"
     CDS             complement(8229..9482)
                     /gene="kch"
                     /locus_tag="RPM73_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDX6521813.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2513 a         2243 c         2391 g         2468 t
ORIGIN      
        1 aattgaaaga tgtttgttaa ggcatggatg caagctatag attctgatac ggtcaataaa
       61 agagaattgc ttaacaattt tgcaaaatgt attggcgagt aagaaccgca tttggtactt
      121 tccgggcaac cgccagacga ttatttattg gtaatgagaa taattaacaa ttaaagagcg
      181 tcgcgaaaga ataatgtgtc tcgacagggg agacacagta cgaatcgaca taaggtgatc
      241 gtctgaatca ccagaataaa taaagtcggt gatagtaata cgtaacgata aagtaacctg
      301 acagcagaaa gtctccgagc ctgtgcaggg tcccaatccg ggattacaca tgctggttaa
      361 taccagtaat tataatgagg gagtccaaaa aacaatgacc aacatcacca agagaagttt
      421 agtagcagct ggcgttctgg ctgcgctaat ggcagggaat gtcgcgctgg cagctgatgt
      481 acccgcaggc gtcacactgg cggaaaaaca aacactggta cgtaacaatg gttcagaagt
      541 tcagtcatta gatccgcaca aaattgaagg tgttccggag tctaatatca gccgagacct
      601 gtttgaaggc ttactggtca gcgatcttga cggtcatcca gcacctggcg tcgctgaatc
      661 ctgggataat aaagacgcga aagtctggac cttccatttg cgtaaagatg cgaaatggtc
      721 tgatggcacg ccagtcacag cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc
      781 gaacactgct tctccgtatg ccagttatct gcaatatggg catatcgccg gtattgatga
      841 aattcttgaa gggaaaaaac cgattaccga tctcggcgtg aaagctattg atgatcacac
      901 attagaagtc accttaagtg aacccgttcc gtacttctat aaattacttg ttcacccatc
      961 aacttcaccg gtgccaaaag ccgctatcga gaaattcggc gaaaaatgga cccagcctgg
     1021 taatatcgtc accaacggtg cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt
     1081 tcttgaacgc agcccgacct actggaacaa cgcgaaaacc gttattaacc aggtaaccta
     1141 tttgcctatt gcttctgaag ttaccgatgt caaccgctac cgtagtggtg aaatcgacat
     1201 gacttataac aacatgccga tcgaattgtt ccagaagctg aaaaaagaga tcccggacga
     1261 agttcacgtt gatccatacc tgtgcactta ctattacgaa attaacaacc agaaaccgcc
     1321 attcaacgat gtgcgtgtgc gtaccgcact gaaactaggt atggaccgcg atatcattgt
     1381 taataaagtg aaagcgcagg gcgacatgcc cgcctatggt tacactccac cgtatactga
     1441 tggcgcaaaa ttgactcagc cggaatggtt tggctggagc caggaaaaac gtaacgaaga
     1501 agcgaaaaaa ctgctggctg aagcgggtta taccgcagac aaaccgttga ccatcaacct
     1561 gttgtataac acctccgatc tgcataaaaa gctggcgatt gctgcctctt cattgtggaa
     1621 gaaaaacatt ggtgtaaacg tcaaactggt taaccaggag tggaaaacgt tcctcgacac
     1681 ccgtcaccag ggtacttttg atgtggcccg tgcaggctgg tgtgctgact acaacgaacc
     1741 aacttccttc ctgaacacca tgctttcgaa cagctcgatg aataccgcgc attataagag
     1801 cccggccttt gacagcatta tggcggaaac gctgaaagtg actgacgagg cgcagcgcac
     1861 agctctgtac actaaagcag aacaacagct ggataaggat tcggccattg ttcctgttta
     1921 ttactacgtg aatgcgcgtc tggtgaaacc gtgggttggt ggctataccg gcaaagatcc
     1981 gctggataat acctataccc ggaatatgta cattgtgaag cactaatggc aatacgtggg
     2041 gcaggagtgt cctgctccac ggtgtctgat ttttatcgca ttacagaagg cacaggccag
     2101 aaggtagggc aatgttaaaa tttattctac gtcgctgtct ggaagcgatt ccgacgctat
     2161 ttattcttat tactatttcg ttctttatga tgcgcctcgc gccgggaagc ccttttaccg
     2221 gcgaacgtac tttaccgcca gaagtgatgg ccaatatcga agcgaaatat catcttaatg
     2281 atccgatcat gacacagtat ttcagctacc tgaaacaact ggcgcacggt gatttcggtc
     2341 catcgtttaa atataaagat tattcggtca atgacttggt tgcatccagt tttcccgttt
     2401 ctgccaaact gggagccgca gcatttttcc ttgcggtaat actgggtgtt agtgctggcg
     2461 ttattgccgc attaaaacaa aacaccaaat gggactatac cgtgatgggg ctggcaatga
     2521 ccggggttgt tatccccagt tttgtggttg caccattatt agtcatgata tttgcgatca
     2581 ttttgcattg gctgccgggc ggtggctgga atggtggggc gcttaaattc atgatattgc
     2641 cgatggtggc gttgtcactc gcttatatcg ccagtattgc gcgtattacc cgtggctcta
     2701 tgattgaagt attacactcc aattttattc gtactgcccg ggcgaaaggg ttacctatgc
     2761 ggcggatcat tttacgtcac gcattaaaac ctgctctgtt acccgtgctc tcttatatgg
     2821 gccccgcatt tgtcggcatt attaccggtt ctatggttat cgaaaccatt tatggtttgc
     2881 cggggattgg acaactgttc gttaatggcg cattgaaccg tgactattcc ttagtgttaa
     2941 gcctgaccat cctggttggt gctttaacca ttttgtttaa tgccattgtc gatgtgctat
     3001 atgcggttat cgacccgaaa atccgttact gatactggag ctcgcgatga tgttaagtaa
     3061 gaaaaacagc gagacgctgg aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt
     3121 gtggcaggac gcacgtcgac gttttatgca taaccgtgcg gcggttgcca gtctgatagt
     3181 gctggtgctg atcgcgttat ttgtaatcct ggcaccgatg ctttcccagt ttgcctatga
     3241 cgatactgac tgggcgatga tgtccagcgc cccggatatg gagtccggtc actactttgg
     3301 tactgactca tccggtcgcg acctgcttgt gcgcgttgcg attggcgggc gtatctcact
     3361 catggtcggt gttgctgcgg cactggtggc tgtggtcgtc gggacacttt atggttcgct
     3421 ttccggttat ctgggcggta aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa
     3481 ctccttccca ttcatgttct tcgtcatttt gctggtgacc tttttcggtc aaaacatcct
     3541 gctgattttc gtggcgatag gcatggtttc ctggctggat atggctcgta ttgtgcgtgg
     3601 gcaaaccctg agtctgaagc gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc
     3661 gacatcgggc attgttattc gccacattgt gccgaatgta ctcggtgtgg tggtggtcta
     3721 cgcatcactt ctggtgccca gcatgatcct ctttgaatct ttccttagct tcctggggtt
     3781 gggtacgcaa gagccgttaa gcagctgggg tgcattgctg agtgatggcg cgaactcgat
     3841 ggaagtctct ccatggttat tgttgttccc agcgggattc ctcgtggtga cgctgttttg
     3901 tttcaacttt atcggcgatg gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt
     3961 gcagccatga gcgtaattga aactgcaact gtgccgctcg cacaacaaca ggctgacgca
     4021 ctgctgaacg tgaaagattt gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg
     4081 gtcaatgatt tgaatttttc cctacgtgcc ggagaaacgc tgggtattgt aggtgagtct
     4141 ggttcgggta aatcgcaaac tgcatttgcg ttgatgggcc tgttggctgc caacggacgt
     4201 attggcggat cggcaacctt caatgggcgt gaaatcctca atttgccaga gcatgaactc
     4261 aataaactgc gcgctgaaca aatctcaatg atttttcagg acccaatgac ttcgttgaat
     4321 ccctatatgc gcgtcggtga gcagttaatg gaagtgctga tgctgcataa gaacatgagc
     4381 aaagctgagg cgtttgaaga gtcggtgcgg atgctcgatg cggtaaaaat gccggaagcg
     4441 cgtaaacgca tgaaaatgta cccgcacgaa ttttctggcg gcatgcgtca gcgagtcatg
     4501 attgcgatgg cattgctatg tcgacctaag ctactgattg cggatgaacc cactactgcg
     4561 ctggacgtca ccgtacaggc gcagatcatg acgctattaa atgaactgaa gcgggaattt
     4621 aataccgcca tcattatgat cacccacgat cttggcgtgg tggcggggat ctgtgacaaa
     4681 gtgctggtaa tgtacgccgg gcgcacgatg gaatatggca atgcgcgcga tgtcttttat
     4741 caacccgttc atccttattc tatcggtttg ctcaacgcgg tgccgcgtct tgatgcggaa
     4801 ggtgaaacaa tgttgaccat cccgggtaat ccgccaaact tgctgcgatt accgaaaggt
     4861 tgcccgttcc agccacgttg tccacatgcg atggaaattt gtagtagcgc accgccgctg
     4921 gaagagttta cgcctggccg tctgcgtgct tgctttaaac cggtggagga actgttatga
     4981 atgctgtaac tgaaggaaga aaagtcctcc ttgaaatcgc cgatcttaaa gtgcactttg
     5041 aaatcaaaga tggcaaacag tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg
     5101 gtgtcactct tcgcctgtat gaaggggaaa cattaggtgt ggtaggggaa tcgggatgcg
     5161 gtaagtccac ctttgctcgc gccatcattg gcctggttaa agcgaccgac ggtcatgttg
     5221 cctggttagg taaagagttg ctgggcatga agcccgatga atggcgtgcc gttcgcagtg
     5281 atattcagat gattttccag gatccgttgg catcgctaaa cccgcgtatg accatcggcg
     5341 agatcatcgc tgaaccactg cgtacttatc atccgaaaat gtcacgccag gaagttcgcg
     5401 agcgcgtgaa ggcgatgatg ctgaaagtcg ggttattgcc taacctgatt aaccgctatc
     5461 cgcatgagtt ctccggtggg cagtgccagc gtatcgggat tgctcgtgct cttattcttg
     5521 aaccgaagct gattatctgc gatgagccgg tttcggcgct ggacgtgtca attcaggcgc
     5581 aggtggtcaa cctgctccag cagctgcaac gtgagatggg attgtcatta atttttatcg
     5641 ctcatgacct ggccgtggta aaacacattt ccgatcgtgt gttggtgatg tatctcggcc
     5701 atgcggtaga actggggacc tatgatgagg tctaccacaa tccactacat ccttacacca
     5761 gggcattgat gtcggcagtc cccatacctg atccggatct ggagaagaac aaaaccatcc
     5821 agttactgga aggggaatta ccgtcgccga tcaacccgcc ttccggttgt gttttccgta
     5881 cccgttgccc gattgccggt ccggagtgcg ccaaaacacg tcctgttctg gaggggagtt
     5941 tcagacacgc cgtttcttgc ctgaaagtcg atccgcttta aacacaataa gggctgacag
     6001 ttgtcagccc tttttcacgc taaaagcgat tatttattcc cgccagatga tatggcaaag
     6061 tttgtgatct ttttcgcggc ataacagaat gcgggcgaaa acatcgttga tttcaccatc
     6121 ttcactgtcc gccagaccaa tcaccacttc ggcaaaaaag tctgggttca aatcgaaatc
     6181 aacatgttcc tgccagtctt ccgccggatc gaataactcc gcgccgccgc gctcttcaaa
     6241 ctgaagattg aacagcagaa catcagctgg atcgagattg tcggcagcca gttcgagaaa
     6301 aatatcgtag gcctgctcaa gcgtttcatc ttcagtcagg cgattgttta gatccatatc
     6361 catagtcact acctgtttaa cctctgttgg cgacgtttta cagcaacgga ctgaagaagt
     6421 aaaacagtcg ctcggcgaca cgttgccata atggacgttt tagccataaa cgggcatcga
     6481 gcagacgtga acgcgaaata taatcgtcct gaacggcggc aaggtctgca ccaaaacctt
     6541 tatcgtcgat tgccagggta atctcgaaat tcagccacag actacgcata tcaaggttaa
     6601 cggtgccaac cagacttagt tcgccatcga ccagcacgct cttggtatgc agtaacccgc
     6661 cttcaaactg ataaatttta accccagcag caagaagttc cgtaaagaat gcgcgactgg
     6721 cccagccgac cagcatcgag tcattttttc gcggaaggat aatactgaca tccaccccgc
     6781 gctgcgccgc cgtgcaaatc gcatgaagta aatcatcgct tggcacaaag tagggcgtgg
     6841 tcatgatcaa atattcacgc gccgaataag ccgcagtcaa taatgcctgg tgaatgagat
     6901 cttccggaaa gccggggcca gaagcaattg tgtgaatggt gtgaccgctg gcctgttcaa
     6961 acggcataat attgacatct ggtggtggcg gcagaatacg ttttccggtt tcaatctccc
     7021 agtcgcagga ataaataatc cccatcgcgg tggcgatggg gccttccata cgcgccatca
     7081 gatcaatcca ttgccctacg cccgcatctt gtttgaagta gcgaggatcg accatattca
     7141 tgctgccggt gtacgcgatg taattatcga tcatgatcat cttgcgatgt tggcgcaggt
     7201 ccatacggcg taaaaacaca cgcatcagat tgacctttaa ggcttcgacc acttcaatac
     7261 cggcattacg cattagctcg ggccacgggc tgcggaaaaa agccacactc ccggcggagt
     7321 cgagcatcaa tcggcaatga ataccacgcc gtgcagccgc cattaatgat tcagccacct
     7381 ggtccgccat gccgccgggc tgccagatat aaaacaccat ctcaatatta tggcgcgcga
     7441 gctggatgtc gcggattaac gcctgcatca catcatctga ctcggtcatc agttgtagct
     7501 gattcccttt gaccccagcg atcccctgac gacgctcgca aagcttgaat aatggcgcag
     7561 cgacactgct attttcttcg gcgaagatat gcttacaggc tttaaggtcg ttaagccatt
     7621 ttgcggtgga aggccacatc gctctggcgc gctcagcgcg gcgtttgcct aaatggagct
     7681 cgccaacggc aagataggca ataattccga ctaacggcag aatgtaaata atcaacagcc
     7741 aggccatcgc ggagggaact gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa
     7801 tgagcaacca gtatcccaga atggccaacc aactcaccaa cgtataaacg gttgtcatag
     7861 attagaaatc cttttgaaag cgcattgtta tgagtttacg catatggatt catctggcaa
     7921 ataaaaacgc gggaaaagca ctggtcagcg tcgggggtgg gggtataatg accattctgt
     7981 tattgcatag agtagttaac atgaagcgga gtagaacgga agtggggcgc tggcgcatgc
     8041 agcgtcaggc tagccgacgt aaatcgcgtt ggcttgaggg gcaatcgcgc cgaaatatgc
     8101 gtatccacag catcaggaag tgcattctaa acaaacagcg taactcgtta ttgtttgcga
     8161 tctacaatat ctaaatatac tgggcaccat tgcggtgccc aataagttta ctctttcgtc
     8221 tttaaactct atttttgcac tgattcttta ctgtcagctt tcgtttcttg ttcatcgtta
     8281 tcgctgaaaa tgccatgacc ggagttcaac aacattgaaa cgagcatatc attattaatc
     8341 tcttcaccat ttaaaactcg cgccagaatt tcgctgccaa acagttgcgg tgagagaatg
     8401 atatccggat gtaccatctt aatcttattc aggtttttgc tatcactgac ggcgagaact
     8461 gttttgacat cactgctcat atcttttgcc gagagtacaa caaacgcgtt atctgcatcg
     8521 ttatcactca gcgccagaat ggcccggcaa cgatcgattc ccgctttctt taatactgaa
     8581 ctgtcattac tgtcaccggg gataacatca gcgttatcgc ctaagcgttg ctcaagttgc
     8641 ttgatatcat cttcaggcaa gttgctgata accgttacgt tttgtccgcg ttgattcagt
     8701 tgcaggatcg tattgatggc gagaatcgaa tgtccgcaaa caataaaatg atctttacga
     8761 tgcattgtat gattgtttcc ttttacaagt ttgttgaatc ccccgcggat aagcggacca
     8821 aaaattgagg tcatagatgt ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg
     8881 aacaatcgtg ctgattcaga aacagggaca atatcgccat agccgacggt tgacatggtt
     8941 tctatcgaaa aatagaacgc ggtcatcaaa ctttctattc gcggattaaa accttcgctt
     9001 aaataaagcg caccgtaggt tgagtaaaac agtaatgtcg tgaaactaat aaatgcaaaa
     9061 attgtcccgg ctgcggcgct actgtgggag aagtctttgc gcagtatcag caaaaacacc
     9121 agcgtaaaaa tgcaaaatcc aatactaaat ttcagccagg gataaaaatg cagggtgtaa
     9181 attagcgcta tcaacaacaa aatgatactg attgcccatg cgagctttgc ccggaacagt
     9241 aagccaatgg agttcaggac aagaaagacg ccgagcataa atagtggggc gtttgccagc
     9301 aaggagagat ccaactctga aaatgctttg atatggaaaa tatcaagcaa attgaccgac
     9361 ataccataga ttgttttaaa aataagcaat ccatttaaaa agacggccag cgcgagaatg
     9421 tcgtgccgta gggtcaccca taaatttgtt gctgtttgtt tgaatgtagc ccagtgactc
     9481 actgatacct ctgtaaccac aattttgttt aattatctta gcgtgcacat tttctgaaca
     9541 tacatgcagc gcgtgaatgt gttaaacaat agtagatgac tttatcggca gtaatcaaag
     9601 gttttgagcg agcaa
//
LOCUS       DAPPNJ010000168         9516 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000168 DAPPNJ010000000
VERSION     DAPPNJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.169"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1040
                     /locus_tag="RPM73_003491"
     CDS             <1..1040
                     /locus_tag="RPM73_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX6521814.1"
                     /translation="NGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNN
                     KKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCL
                     WAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSK
                     LFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVD
                     INQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAK
                     IIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKS
                     EGGNKVIIHHI"
     gene            1221..2711
                     /locus_tag="RPM73_003492"
     CDS             1221..2711
                     /locus_tag="RPM73_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX6521815.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2754..3257
                     /gene="yeaK"
                     /locus_tag="RPM73_003493"
     CDS             2754..3257
                     /gene="yeaK"
                     /locus_tag="RPM73_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDX6521816.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3258..3362)
                     /locus_tag="RPM73_003494"
     CDS             complement(3258..3362)
                     /locus_tag="RPM73_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521817.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3532..3978
                     /locus_tag="RPM73_003495"
     CDS             3532..3978
                     /locus_tag="RPM73_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDX6521818.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAASILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3935..4756)
                     /gene="nimR"
                     /locus_tag="RPM73_003496"
     CDS             complement(3935..4756)
                     /gene="nimR"
                     /locus_tag="RPM73_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDX6521819.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4853..6034
                     /gene="nimT"
                     /locus_tag="RPM73_003497"
     CDS             4853..6034
                     /gene="nimT"
                     /locus_tag="RPM73_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDX6521820.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6089..6436
                     /locus_tag="RPM73_003498"
     CDS             6089..6436
                     /locus_tag="RPM73_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDX6521821.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6458..6712)
                     /gene="yoaF"
                     /locus_tag="RPM73_003499"
     CDS             complement(6458..6712)
                     /gene="yoaF"
                     /locus_tag="RPM73_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDX6521822.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6895..7920
                     /gene="dgcP"
                     /locus_tag="RPM73_003500"
     CDS             6895..7920
                     /gene="dgcP"
                     /locus_tag="RPM73_003500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDX6521823.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7953..8051
                     /locus_tag="RPM73_003501"
     CDS             7953..8051
                     /locus_tag="RPM73_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDX6521824.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8054..8128
                     /gene="yoaJ"
                     /locus_tag="RPM73_003502"
     CDS             8054..8128
                     /gene="yoaJ"
                     /locus_tag="RPM73_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDX6521825.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8187..8435)
                     /locus_tag="RPM73_003503"
     CDS             complement(8187..8435)
                     /locus_tag="RPM73_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDX6521826.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8662..>9516)
                     /locus_tag="RPM73_003504"
     CDS             complement(8662..>9516)
                     /locus_tag="RPM73_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX6521827.1"
                     /translation="KLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYIS
                     IQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSR
                     KVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSA
                     AGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCAC
                     CGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
BASE COUNT         2316 a         2318 c         2256 g         2626 t
ORIGIN      
        1 ttaacggcag tacactgatc cagacaaagt cgaatgatgt ttctattttc tatattttcc
       61 gccaactcag ttttatttgt ttaacctcgc tggcactctt ttgttatgga aaagacaaca
      121 tccttgacaa caataagaaa aaaacgggaa tcctgttgct ggcgctgatt ccttttttag
      181 tttttcccct tctggcacac aatctgagca gttataacgc tgactattct ttgtatgtcg
      241 tcgactactg tccggacaac catactgcga cctggggaat caactataca aaaatattgg
      301 tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta gccagcgaac
      361 tatggccatt aatagcatta ttatgtctgg catcgctatg ctgcaactta cttctactga
      421 ctctggatga gtataattac accatctggt atatcagtcg cgggattgaa gtttccagta
      481 aactgtttgt tgtgtctttt ctgatttata acatttttca ggagctgcaa ctctccagca
      541 aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt ttcaacagcg
      601 tagagtcatt attgtcgcga cctgttgtta aggacttctg tgtcatgctg gttgatatta
      661 atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa gtgctggttt
      721 caattgtcga tattatccag caaagcatcc gccccgatga tattttagcg cgtctggagg
      781 gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg
      841 aacgtatgcg taaaaatgtc gaactcctga ccggctttag taacagatat gatgttcctg
      901 aacaaatgac catcagtatt ggcacggttt tttcaacggg tgacacgcgt aatatctcgc
      961 ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag
     1021 tgattattca tcatatttaa gtgcaaaaat attcagagcc atgctttttg cgtggctttt
     1081 gcatacaatt tattactatt acccctaaat ttcccctccc cagcggcgca gagatgagta
     1141 taattagcgc ccttgtgcca ggccgcaatc gaactttatc tggttttctc gtttcactaa
     1201 ccgaaggagt gccatttatc atgaaattgc accatagaat gctccggcat tttatcgccg
     1261 caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc agcgacagag
     1321 caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt ctttatgata
     1381 agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg
     1441 aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc aataataccc
     1501 atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta caaaccgcat
     1561 ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt gatcaggcag
     1621 tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc gaagagaaat
     1681 ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg gttaatgttg
     1741 aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc atcgatcgca
     1801 aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac caggatcagc
     1861 gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg cagctaaaag
     1921 ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac actcatgacc
     1981 gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg gggcgcgaca
     2041 ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc catgacttac
     2101 cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg tcttcgtgga
     2161 aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtg cggatgcact
     2221 tccgcttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact ggactgtata
     2281 atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg caatccggtt
     2341 cttcggtgat gtttattgca attgacatgg acaagttaaa gctaataaat gacaccctcg
     2401 gtcatcagga gggggattta gcgattacgt tgttagctca ggcgattaaa caatcgattc
     2461 gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt
     2521 cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg caacatatcg
     2581 cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa aacgatacgt
     2641 tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag aacaaaaaca
     2701 gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg
     2761 aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca caagaaggtg
     2821 ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg tcagaaattc
     2881 gtggcaccgc tctaggccag ggtgctaagg cactggtatg taaagtcaaa ggcaatggcg
     2941 taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg agccaactcg
     3001 ccagccatat aggcggatta cgcgcttcgt tggccagccc ggcggaagtc gatgaactca
     3061 caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc aaactggttg
     3121 ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg atgctggata
     3181 aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa ctgctcaatt
     3241 tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa aaccagcaca
     3301 acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat ttgatcgttc
     3361 atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac aaatggcagt
     3421 ttgatgacag ttcgcctgat tttttatgca aaaaacgtaa agattttcta cttccttcct
     3481 gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat
     3541 gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact
     3601 gtcgccgctt caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc
     3661 ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg
     3721 gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg
     3781 aaatcgctgg tggcaattgc agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg
     3841 accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac tgttttaggc
     3901 gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct ggtttcgctg
     3961 attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt
     4021 tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct
     4081 gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac tgctgacgcc
     4141 actggcgaaa actcaacccc gtctctttta ctatcagtcg cgcaagattg cgttcactca
     4201 tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct ttcgccatca
     4261 tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg
     4321 gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc ggctcagcac
     4381 gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt
     4441 tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac agaaaacaga
     4501 gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc caaaccgcat
     4561 attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg
     4621 ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg
     4681 cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat
     4741 tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa
     4801 cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg
     4861 ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc
     4921 cacaacatta cgcgtcacct ttaccggcgc agcgccgtta ctggatacga ttcgttccgc
     4981 ttactcgctg acgacagcgc aaaccggctt attaaccacc ctgccattat tggcctttgc
     5041 gctaatctca cctttggctg ccccggtggc gcgacgtttt ggtatggaac gtagcctgtt
     5101 tgccgcgtta cttttgatct gtgcaggtat cgcaattcgt tctctccctt cgccttactt
     5161 attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc
     5221 aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct
     5281 gacaatggga gctgcagcgg cactgggatc ggctatggtc gtgccgctgg ctttgaacgg
     5341 ttttggctgg caaggcgcgt tgttcatgct gatgtgtttt cctctgctgg ctcttttttt
     5401 atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca
     5461 tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggatcaa
     5521 ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca gtcgcggcta
     5581 tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg
     5641 tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg cagcgttcgt
     5701 tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac
     5761 ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat
     5821 tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     5881 cgggtatttg ctggcagcct gtgggccgcc gctgatgggt aaaatacacg atgctaacgg
     5941 tgactggtcc gtaccactga tgggtgttgc catactttca ctactgatgg cgattttcgg
     6001 actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt gacgtgacaa
     6061 atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     6121 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     6181 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     6241 caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat atcttgcaga
     6301 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct
     6361 gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg
     6421 gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca gccggatggt
     6481 cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caattgttgc
     6541 tgaccccttg cgctgtctgc actggaatga gcgtcccgcc cttctgctgg caatagaccg
     6601 acgccggatt tgccatacca atctgcggcg gtttaggtgc ttctggctga gaagaggttg
     6661 aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc atttattgat
     6721 ctcacacatt tatctacgct tagagtatcg cggtatcgtt ttgttttgca gcactatttt
     6781 tattacattc actcaaaaca tattacgtct tgtttcatct ttgttgatga tgttttatca
     6841 tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg tttcgtgtca
     6901 gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg
     6961 gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac ctacctaacc
     7021 aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag ccagaaaatg
     7081 cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg cgctattgat
     7141 gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat tgcggcacgc
     7201 aatcttggaa tcaccacatt tctgagtaca ccaattcact taccggatgg atcattctat
     7261 ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt
     7321 ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcgtt ggttgaacag
     7381 ctgcgcgaag ccaatgccgc gctgattgcg caatcgtata ccgactcgtt aaccgggcta
     7441 ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc ccggcatctt
     7501 aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat caatgaccgt
     7561 tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct taatacgctc
     7621 cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt tgtttcacta
     7681 aacaacgaga atgcggatat ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga
     7741 gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg catagtagaa
     7801 gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat tgcgatgtat
     7861 caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc atccagcgct acattcctga
     7921 ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt tttccggttg
     7981 taatctttat tacagcggtc gtatttttag catggttttt tattggcggc tatgctgccc
     8041 cgggagcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga ttattgtcgt
     8101 tctcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg
     8161 tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat aaatagcacg
     8221 acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc gactttacca
     8281 aagccaaaca gtgtgctgat ccatccgccg actacggcac cgactatccc cagcagaata
     8341 gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag aataccggca
     8401 ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat ctgttttgca
     8461 ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa gatttgtgac
     8521 gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac gatcggtgat
     8581 aattaccaac cacaaacatc atgttcgttt tccgtgtctt gacatcctcc acgccctgaa
     8641 tgacgaggat ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
     8701 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
     8761 aatgttacga gcgccgttta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
     8821 ttgactttgt gacaggcgat tttctttcgc tgtatgacca cagcacgcgc aacgctggct
     8881 tgtgtacgct ggcggaacag caagcacctg accgccactc cagagctgct tgtactcaag
     8941 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
     9001 ccggacattg cgccccggct ggctgaccgt acccgctgct gactttgaca tgttgctgac
     9061 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttatg
     9121 aaggtagtcc ctgcggatat ttgcgatacg ggaatgcagt cgctgtattt tgcgtttctg
     9181 cttctgccag ttattgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
     9241 cgtcttctgg tttttctgaa aactgtttac aggcccaaag actgtgccat ctgacagcgt
     9301 ggcgagttta gccacgccag catccagccc gatcattgat gctgaagggt gaaccggagt
     9361 tgatacttca ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgaccgt
     9421 gacatttttc acaacacccg tgacctgacg gctattccgg tagcgcatcc agcccagttt
     9481 cggcagaaaa atacggctgt tttcctgatc gagctt
//
LOCUS       DAPPNJ010000169         9513 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000169 DAPPNJ010000000
VERSION     DAPPNJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.170"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(1..213)
                     /gene="cspA"
                     /locus_tag="RPM73_003505"
     CDS             complement(1..213)
                     /gene="cspA"
                     /locus_tag="RPM73_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDX6521828.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(494..784)
                     /locus_tag="RPM73_003506"
     CDS             complement(494..784)
                     /locus_tag="RPM73_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDX6521829.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1218..1928
                     /locus_tag="RPM73_003507"
     CDS             1218..1928
                     /locus_tag="RPM73_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDX6521830.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1978..2952)
                     /gene="ghrB"
                     /locus_tag="RPM73_003508"
     CDS             complement(1978..2952)
                     /gene="ghrB"
                     /locus_tag="RPM73_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDX6521831.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3056..3715)
                     /locus_tag="RPM73_003509"
     CDS             complement(3056..3715)
                     /locus_tag="RPM73_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDX6521832.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3868..6202
                     /gene="bisC"
                     /locus_tag="RPM73_003510"
                     /pseudo
     CDS             3868..6202
                     /gene="bisC"
                     /locus_tag="RPM73_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
     gene            complement(6171..6611)
                     /locus_tag="RPM73_003511"
     CDS             complement(6171..6611)
                     /locus_tag="RPM73_003511"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX6521833.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6608..7171)
                     /gene="tag"
                     /locus_tag="RPM73_003512"
     CDS             complement(6608..7171)
                     /gene="tag"
                     /locus_tag="RPM73_003512"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDX6521834.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7329..8027
                     /locus_tag="RPM73_003513"
     CDS             7329..8027
                     /locus_tag="RPM73_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX6521835.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8256..9458
                     /locus_tag="RPM73_003514"
     CDS             8256..9458
                     /locus_tag="RPM73_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6521836.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2238 a         2337 c         2481 g         2457 t
ORIGIN      
        1 ttacaggctg gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga
       61 cactttctga ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac
      121 gaacacgtct ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa
      181 ccattttacg ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt
      241 gcctttcggc gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg
      301 aggggtatca acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt
      361 acgtcaaacc gttgatgtgc attaagccac gcattggcgg gtgatgcaac aattattttt
      421 catatttatg attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa
      481 agtggataaa agtctattcc atcaactgct tacttaatgc cgggttggct tgaatcaaac
      541 gcatcaattt tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca
      601 tggcgactga tacgcccaaa acccgggcga aatcatcgat ttttaaccct gtcccttttc
      661 gtaactgctc aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt
      721 catctttaaa aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt
      781 ccattgaaaa ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga
      841 atagtcggga aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga
      901 ttcagccagt catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat
      961 ctcttaccac tcctggtttg taaagatttc gaaaatctaa ccttctgcga aggcgtaaat
     1021 ctctggctac aaaaaaagca acggcactat cattttttaa caatgaaaca acaaattaac
     1081 cttgtttcat tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag
     1141 cctgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg
     1201 gtgaaaggag taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg
     1261 cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta
     1321 aagcgtttat cgacttcctg cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc
     1381 tgactgccga tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg
     1441 gttattctca gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg
     1501 ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga
     1561 acggttcgct gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg
     1621 cgcactctgc gctaaaacag accgatgatt tgaaaccggt ctttgatcag gcattcacca
     1681 aagtggtaac aacgccagct gatgcattgc aaccgttgat tccggctgca caaactttca
     1741 cgcaacaact ggtgatggtt ggagactaca ttgctcaaca aggtactcag gtgagctttg
     1801 tcgcaaatgg catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg
     1861 cgccattacc ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa
     1921 cccaataaag agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcggctta
     1981 gtccgcgacg tgcggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa
     2041 attatccacg gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg
     2101 cggtactgcg acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg
     2161 ttcgaagaca tccagcccgg cggcgtgaat ttcgcctttc tgcaatgcag caatcagtgc
     2221 attttcatca accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt
     2281 ggcgaattgt tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca
     2341 aacgaaatct gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc
     2401 ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg
     2461 cgcacgttgt gccagcgcca taccgatccg tcccatcccg acaatgccca gtgttttatg
     2521 gtgaacgtca gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac
     2581 ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc
     2641 ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag
     2701 cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg
     2761 cattttttcc agcaatgcgg catcaacatt ctcgtttgaa cccagtaaac cttcagcttc
     2821 ggcaaaaatt gctgcatttt gttcgacggt ttgtgggctg aggtttgcca cctggtgaac
     2881 ggtgaaatgc tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac
     2941 ggacggcttc atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga
     3001 tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca
     3061 gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc
     3121 tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct
     3181 gggtgatcaa cgcgctggca acggaatccg cacgttgctg ggagagacgc atgttcaggt
     3241 cgtgaccacc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt
     3301 tcagcaccat tgccacgccg gtcagggtgt tagcgcccgc aggtttcagg gtcgcgctgc
     3361 tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta
     3421 cgctaacacc agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat
     3481 aacccacgcc gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc
     3541 gatctttctt cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg
     3601 cgccgatagc agatttacct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag
     3661 ataccgccag agcgccactc actacggcgg caataagata aacacgtttc ttcattgtta
     3721 atccttaata acctttttat tctttgccac gggttccgtg gcgggagatt atgccgcgtg
     3781 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     3841 acatctaacc tttcaggagt tttcggtttg gccaactcat cctcacgata ttccgttctg
     3901 actgccgccc attgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     3961 catggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4021 cacagcaata cacgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4081 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4141 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4201 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4261 cgctatatgg cgctggcagg cggttatatc gggcatctgg gggattattc gaccggtgcg
     4321 gcacaggcga tcatgccgta tgtcgtgggt ggcagcgaag tttatcaaca gcaaaccagt
     4381 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     4441 acgctgaaaa ttgcgtggaa tgcatctgat gagcaggggc tttcttactt ttccgcactg
     4501 cgtgacagcg ggaaaaagct gatctgtatt gatccaatgc gatcggaaac cgtcgatttc
     4561 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     4621 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct gacgcgttgc
     4681 accacaggtt atgccgtctt cgcctcttaa tttgctgggc gagagtgacg gaatagcgaa
     4741 aaacgccgaa tgggcggcag agatttgtgg ggttaacgca gcgaaaatcc gcgagctggc
     4801 ggctcttttc caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca
     4861 gtttggtgag caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg
     4921 cacacccggc ggcggttttg gtctttctta tcattttgcc aatggtggta accccacgcg
     4981 gcgttctgcg gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga
     5041 taaaatccct gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca
     5101 caacggtatg aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa
     5161 ctttactcat catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt
     5221 ggtgatctct gaatgcttct ggacggcggc ggcgaaacac gcggatatcg ttctgcctgc
     5281 gactacctct tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct
     5341 ggtgccgatg aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt
     5401 tgccgagttg agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag
     5461 tcagctgcaa tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca
     5521 ggttgaattg ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga
     5581 aaacccggac agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca
     5641 tccgttaaaa accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg
     5701 ttacccggat tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc
     5761 cgaaccagaa cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct
     5821 gaattacagt tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca
     5881 tcctgacgat gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc
     5941 ccgtgggcag atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg
     6001 cattcacgaa ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg
     6061 cgcagtgaac gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg
     6121 taatacggcg ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt
     6181 tgaaccaccg gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa
     6241 tcgacaatgt gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa
     6301 acctccagca tcagatgggg atagcgctgc tgtacatact gcatcagcgc cttaccaata
     6361 ccgcgtctga cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg
     6421 ctgacaaaac cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga
     6481 taggcatccc gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc
     6541 ccccaggttg tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct
     6601 tcccgaatca tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca
     6661 gcccacatgc ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt
     6721 ttttcagtgc cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg
     6781 ttgtggcttg tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg
     6841 gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt
     6901 tccctcgatg gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca
     6961 ttgctgcgac cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt
     7021 tgaggacggt gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca
     7081 gttttttact gtcagtttca ggcacgcccc attcattatc atggtaggca atataaagcg
     7141 ggtcctgact cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa
     7201 aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac
     7261 tatgcccgat atccttcatg caatgacaat aatattgccg aattcggctc ttccctggag
     7321 tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg
     7381 tggtaatcag tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg
     7441 atacgcaacc cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt
     7501 acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca
     7561 atctggcgga agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc
     7621 ctttatatga acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca
     7681 cctcgatgta taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg
     7741 ccagcgtgag tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg
     7801 cgcaaatcag ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga
     7861 gccggatgac ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata
     7921 tgttgctcaa tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta
     7981 ataatagcga ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca
     8041 attgaaacca ttgaataaca acagttgtgg cgatagtggg tgctaactta ccaaataata
     8101 aatttggtga ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg
     8161 atggcatttc atgccgtttt tccccaggca taaaatgcac ttcgttatgg ttgtcggcag
     8221 agatttttcc tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta
     8281 cccgctggct gacactcatc ggtactatca ttacccagtt tgcactgggg tcggtttata
     8341 cctggagcct gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg
     8401 ctttctcttt cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaaat
     8461 tgcaggaacg ttttggcgtt aaacgcgtca ccatggcttc cggcattttg ctgggattag
     8521 gcttcttcct gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc
     8581 tggtcggtct ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt
     8641 tcccggagcg taaaggtctg atctccgcgt tcgctatcgg ttcttatggt ctgggtagcc
     8701 tgggtttcaa atttatcgac acgcagctgc tcgaaacggt gggtttggaa aaaacatttg
     8761 tgatttgggg agcgattgcg ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg
     8821 caccaaaaca ggaagtgaaa accagcaatg gtgtggtgga gaaagattac acgctggcag
     8881 agtcgatgcg taaaccgcag tactggatgt tagcggtaat gttcctgact gcctgcatga
     8941 gcggcctgta cgtgattggg gtagcgaaag atatcgccca aagtctggca caccttgatg
     9001 tggtttccgc agccaatgca gtaacggtta tttccatcgc taacctttca ggtcgtctgg
     9061 tgctggggat actgtctgac aaaattgccc gtatccgtgt tatcaccatt ggtcaggtga
     9121 tttcgctggt gggtatggcg gccctgctgt ttgcaccatt aaatgcagtg acgttctttg
     9181 cagcgattgc ctgcgtggca tttaactttg gcggcactat taccgtcttt ccgtcactgg
     9241 tcagtgagtt cttcggcctc aataacctgg cgaaaaacta cggtgtgatt tacctcggtt
     9301 tcggtatcgg tagcatttgt ggttcgatta tcgcctcact gtttggcggc ttctatgtga
     9361 ccttctacgt gattttcgcc ctgctgattc tgtcattggc gctttctacg acaattcgtc
     9421 agccagagca gaaagtattg cgtgaggcgc atatttagaa atatgttgct actgaaataa
     9481 atgcgaggag ggagacttca aagtctccct ttt
//
LOCUS       DAPPNJ010000170         9410 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000170 DAPPNJ010000000
VERSION     DAPPNJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.171"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            206..370
                     /locus_tag="RPM73_003515"
     CDS             206..370
                     /locus_tag="RPM73_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDX6521837.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            373..540
                     /locus_tag="RPM73_003516"
     CDS             373..540
                     /locus_tag="RPM73_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521838.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            654..749
                     /locus_tag="RPM73_003517"
     CDS             654..749
                     /locus_tag="RPM73_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521839.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            913..3588
                     /gene="acnA"
                     /locus_tag="RPM73_003518"
     CDS             913..3588
                     /gene="acnA"
                     /locus_tag="RPM73_003518"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDX6521840.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3652..4242)
                     /gene="ribA"
                     /locus_tag="RPM73_003519"
     CDS             complement(3652..4242)
                     /gene="ribA"
                     /locus_tag="RPM73_003519"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDX6521841.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4412..5176
                     /gene="pgpB"
                     /locus_tag="RPM73_003520"
     CDS             4412..5176
                     /gene="pgpB"
                     /locus_tag="RPM73_003520"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDX6521842.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5325..5633
                     /gene="lapA"
                     /locus_tag="RPM73_003521"
     CDS             5325..5633
                     /gene="lapA"
                     /locus_tag="RPM73_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDX6521843.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5640..6809
                     /gene="lapB"
                     /locus_tag="RPM73_003522"
     CDS             5640..6809
                     /gene="lapB"
                     /locus_tag="RPM73_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDX6521844.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7002..7739
                     /gene="pyrF"
                     /locus_tag="RPM73_003523"
     CDS             7002..7739
                     /gene="pyrF"
                     /locus_tag="RPM73_003523"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDX6521845.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7739..8065
                     /gene="yciH"
                     /locus_tag="RPM73_003524"
     CDS             7739..8065
                     /gene="yciH"
                     /locus_tag="RPM73_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDX6521846.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8191..8409)
                     /gene="osmB"
                     /locus_tag="RPM73_003525"
     CDS             complement(8191..8409)
                     /gene="osmB"
                     /locus_tag="RPM73_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDX6521847.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8678..>9410)
                     /locus_tag="RPM73_003526"
     CDS             complement(8678..>9410)
                     /locus_tag="RPM73_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX6521848.1"
                     /translation="TILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSYLRRA
                     HGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLGEQ
                     KKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAFIG
                     IDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNRVI
                     TDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2340 a         2206 c         2450 g         2414 t
ORIGIN      
        1 ttttatccct catatttata gggtaaatta cctgtaaagt agtgtctaat tacccgcctc
       61 gcaaattcca gttttcaaat atctatttca ggtcaaaaga ttgaattatt cactggagac
      121 gattcgtaaa ttcgctggat ttttgactaa agtttcttta ggatttgtct catcgtataa
      181 ttaattacac caatcgttca acgtcttgct gataagtgat ggcgattata tgaggttagc
      241 aatgccttct ggaaatcagg aaccccgccg cgatcctgaa ttaaaacgta aagcctggct
      301 ggcggttttt cttggttctg cacttttctg ggtggttgtc gcactgctga tttggaaagt
      361 gtggggataa ctatggtcgg tcaggagcaa ctggagtcgt caccattatg ccagcatagt
      421 gacaatgaaa cagaaacgaa acgggaatgt tccgtcgtta ttccagacga ctggcaacta
      481 acatcgcagc agcaagcctt tatagaactg tttgctgaag atgatcagcc gaaacaataa
      541 ttatcatcat tcttattacc catttttaat gaattacagg gcttttaata caccgcagca
      601 ataacaggtt gagttatctc aacacaaagt aataaccgtt aagggtgtag cctatgatca
      661 acacaaatat gaaatattgg tcctggatgg gcgcattttc tctgtcgatg ctcttctggg
      721 ccgaactcct ctggatcatt actcactgat ccctgacccc gctgcggcgg ggttgtcatt
      781 tgctttgcca caaggtttct cctcttttat caatttgggt tgttatcaaa tcgttacgcg
      841 atgtttgtgt tatctttaat attcaccctg aagagaatca gggcttcgca accctgtcat
      901 taaggaggag ctatgtcgtc aaccctacga gaagccagta aggacacgtt gcaggccaaa
      961 gataaaactt accactacta cagcctgccg cttgctgcta aatcactggg cgatatcacc
     1021 cgtctaccca agtcactcaa agttttgctc gaaaacctgc tgcgctggca ggatggtaac
     1081 tcggttaccg aagaggatat ccacgcgctg gcaggatggc tgaaaaatgc ccatgctgac
     1141 cgtgaaattg cctaccgccc ggcaagggtg ctgatgcagg actttaccgg cgtacctgcc
     1201 gttgttgatc tggcggcaat gcgcgaagcg gttaaacgcc tcggcggcga tactgcaaag
     1261 gttaacccgc tctcaccggt cgacctggtc attgaccact cggtgaccgt cgatcgtttt
     1321 ggtgatgatg aggcatttga agaaaacgta cgcctggaaa tggagcgcaa ccacgaacgt
     1381 tatgtgttcc tgaaatgggg aaagcaagcg ttcagtcggt ttagcgtcgt gccgccaggc
     1441 acaggcattt gccatcaggt taacctcgaa tatctcggca aagcagtgtg gagtgaattg
     1501 caggacggtg aatggattgc ttatccggat acactcgttg gtactgactc gcacaccact
     1561 atgatcaacg gccttggcgt gctggggtgg ggcgtgggtg ggatcgaagc agaagccgca
     1621 atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa acttaccggt
     1681 aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca aatgctgcgc
     1741 aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga ttcactaccg
     1801 ctggcggatc gcgccaccat tgccaatatg tcgccagaat atggtgccac ctgtggcttc
     1861 ttcccaatcg atgctgtaac cctcgattac atgcgtttaa gcgggcgcag cgaagatcag
     1921 gttgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc gggcgatgaa
     1981 ccgattttta ccagtacgtt agaactggat atgaatgacg ttgaagcgag cctggcaggg
     2041 ccaaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt tgccgccagt
     2101 aacgaactgg aagtgaatgc cacgcataaa gatcgccagc cggtcgatta tgttatgaac
     2161 ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac ctcgtgcacc
     2221 aacacctcta acccaagtgt gctgatggct gcaggcttgc tggcgaaaaa agccgtaact
     2281 cttggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccaggctc gaaagttgtt
     2341 tctgattatc tggcaaaagc gaaattgaca ccgtatctcg acgaactggg gtttaacctt
     2401 gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga tcctatcgaa
     2461 acggcaatca aaaaaggcga tttaaccgtc ggtgcagtgc tgtccggcaa ccgtaacttt
     2521 gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc gctggtggtt
     2581 gcctatgcgc tggcgggaaa tatgaatatc aacctggctg ctgagcctat tggtcatgat
     2641 cgtaaaggcg acccagttta tctgaaagat atctggccat cggcacaaga aattgcccgt
     2701 gcggtagaac aagtctccac agaaatgttc cgcaaagagt acgcagaagt ttttgaaggc
     2761 acagcagagt ggaaggaaat taacgtcaca cgatccgata cctacggttg gcaggaggac
     2821 tcaacctata ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc agcaccagtg
     2881 gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac cactgaccat
     2941 atctctccgg cgggcagtat taagcccgac agcccagcgg gtcgatatct acaaggtcgg
     3001 ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca tgaagtgatg
     3061 atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg
     3121 gggatgacgc ggcatttacc tgacagcgac gtagtctcta tttatgatgc tgcgatgcgc
     3181 tataagcagg aacaaacgcc gctggcggtg attgccggga aagagtatgg atcaggctca
     3241 agccgtgact gggcggcaaa aggtcctcgt ctgcttggaa ttcgtgtggt gattgccgaa
     3301 tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcctgcc gctggaattt
     3361 ccgcaaggcg taacgcgtaa aacgttagga ctaaccgggg aagagaagat tgatattggc
     3421 gatctgcaaa atctacaacc cggtgcgacg gttccggtga cgcttacgcg cgcggatggt
     3481 agccaggagg tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt gacctactac
     3541 cagaacgacg gcattttgca ttatgtcatt cgtaatatgt tgaagtaaca agtatttgct
     3601 tgccggttat tttgcttccg gcaagcaaac taattacaca atgcaagagg gttatttgtt
     3661 cagcaaatgg cccattttct cggctttggt atcgagataa tgttcgttat tggggttacg
     3721 acctacaatc aatggtacgc gttcaacaat attaatccct gcttcggtca gaatttcgac
     3781 ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt tgaacatatc
     3841 agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt tagcctctac
     3901 ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca gaccaatgtt
     3961 acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa tttgcgtcaa
     4021 tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt caccggtcag
     4081 acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc catagactag
     4141 cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga aatcgcccca
     4201 tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct ccagataatg
     4261 ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat cacgacgcgt
     4321 cgatcgttca cggaatggca caaatctgtc aatttttcct ggaactggcg ttttcagtta
     4381 tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgccagac gtaccgcagt
     4441 gggagctgca ctattgcttg tcatgccagt agccgtatgg atttctggct ggcgttggca
     4501 acctggagaa caaagttggc tactaaaagc ggctttttgg gttactgaaa ctgtcaccca
     4561 gccctggggc gtcattacac atttgatttt attcggctgg tttctctggt gtctgcgttt
     4621 tcgcattaag gctgcctttg tattatttgc cattctggcg gccgcaatcc ttgtgggaca
     4681 aggcgttaaa tcctggatca aagacaaagt ccaggaacca cgaccttttg ttatctggct
     4741 ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag cagaacgcgg
     4801 aaatttagtg aaagaacagt tggctgaaga gaaaaatatc ccacaatatt tgcgttcaca
     4861 ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg ctgccagttg
     4921 ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca ttgctatctt
     4981 gctggtctgg gcaacgggag tcatgggaag ccgcctgctg ctcgggatgc attggccacg
     5041 cgatctggta gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa cgtggcttgc
     5101 gcaacgaatt tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa tagcgcaacg
     5161 agaacaagaa agttaacgct ggttgatttt ccgaatttag cccttaaatc atcaacaatg
     5221 cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct attgcggtaa
     5281 gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa tatttactca
     5341 ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg cagaacgatc
     5401 aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc acattgctgg
     5461 cggtattgtt tgctgcgggg tttgctatcg gttggttgat ttgtggcctg ttctggctgc
     5521 gagttcgtgt ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt
     5581 cacccgcgac tgacgtggct gtagtgccgc actcgtcagc ggcgaaggaa taactttcta
     5641 tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg tatatgggcc
     5701 gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt gattacgtag
     5761 cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg tttctcgata
     5821 tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac ctgttccgtt
     5881 cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa agcgcctcgc
     5941 tgacctatga acagcgtctg ttggcgattc aacaactagg gcgtgattac atggccgccg
     6001 ggttatacga ccgcgcggaa gacatgttca atcagctgac cgatgaaact gacttccgca
     6061 ttggcgctct gcaacagttg ctacaaatct accaggctac cagtgagtgg cagaaagcaa
     6121 ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc
     6181 atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat cgtgccatga
     6241 ccttgctaaa aaaaggggcg gcggcagata aaaacagcgc ccgcgtatcc atcatgatgg
     6301 gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg caacgcgtga
     6361 tatcccagga cagagaactg gtcagcgaaa cgctggaaat gttgcaaacc tgctatcagc
     6421 agttgggtaa aactgccgaa tgggcagaat tcctgcaacg cgcggtggaa gagaacaccg
     6481 gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt agtgaggccg
     6541 cacaggtcta tattacgcgc cagcttcagc gtcatccgac catgcgtgtg ttccataagt
     6601 taatggatta ccacttaaat gaagcggaag aagggcgtgc caaagagagc ctgatggtgc
     6661 tgcgtgacat ggttggcgaa aaggtacgta gtaagcctcg ttatcgctgc cagaaatgtg
     6721 gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgggcctgg tcaaccatta
     6781 aaccgattcg cggtcttgat ggcctgtaat ttttaaaaaa atcctacttt agttacaaca
     6841 tactagttat tgatgttcca ttgtgctccg gcaacgacgg cgcagaaaaa gcctgccagg
     6901 ggaaaaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc gtttacctgt
     6961 ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta actgcttcat
     7021 cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat cataatcgtg
     7081 atgacgcgct gtcctttgtc gacaagatcg acccacgcga ttgtcgtctg aaggtcggca
     7141 aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag cgtggttttg
     7201 atatctttct tgacctgaaa ttccacgata ttcctaacac tgcagcgcac gctgtcgctg
     7261 ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctctggtggg gcgcgtatga
     7321 tgaccgccgc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt ttgattgctg
     7381 tgacagtgtt gaccagcatg gaagccagcg acctggtcga tcttggcatg acactgtcac
     7441 ctgcagatta tgcagaacgt ctggcggcac tgacgcaaaa atgtggcctt gatggtgtgg
     7501 tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag ttcaaactgg
     7561 ttacgccggg cattcgtccg caggggagtg aagctggtga ccagcgccgc attatgacgc
     7621 cagaacaggc gttgtcggct ggtgttgatt atatggtgat tggtcgcccg gtaacgcaat
     7681 cggtagatcc agcgcagacg ctgaaagcga tcaacgcctc tttacagcgg agtgcatgat
     7741 gagtgattcc aacagccgtc tggtctactc aacggaaacc ggacgtattg atgagcccaa
     7801 agcggcccct gtacgcccta aaggcgacgg tgtggtgcgt attcagcgtc agaccagtgg
     7861 acgtaaaggt aagggcgttt gcctgattac cggtgtcgat ctcgatgatg ccgaactgac
     7921 aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta aagatggagt
     7981 tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag cgaaagggat
     8041 gaaggtaaaa ctcgcaggcg gttaacataa aaagccacgg atatatccgt ggctttcgaa
     8101 tattttactg tgcgtattat tcagttattt cgtactgaac agaatcaagc gtattataat
     8161 tgcgagtcga taatcgacat ttatttacga ttatttaccg acctggtgac caataacacc
     8221 accgacagct gcaccaccta atgtacccaa cgtactgcca tcggtcagta ctgcaccgcc
     8281 taatgcccct gcacccgcgc cgattgcggt gttgcggtcc cgtttagacc agttagaaca
     8341 ggcactcaga gacattgcca aagtaattgc cagaacagcc gcggtcattt ttttgctcgt
     8401 tacaaacata atactctctc ctgaatttat gattcacgga agtaagctct ctataactat
     8461 aatagctaag aataagcctg gtgaaattat tccgtgaaat ctgctcgcgc gcagggttat
     8521 atcacgcagg tgataatgac ttcctgttat atcgctgata ataattttat atcttgagag
     8581 tgttaataac aggtaaatag tcttaattat caaccaggaa tcatcttaga gcggatgatt
     8641 tgccaaactg caaatcatcc gtagagaagg gaaatggtta aatgtcaacg acgtgaacgg
     8701 tgagtttact gtgctcaaga tgcatcagat cgctggcgcg tattttcgaa tcggtaatca
     8761 cgcgattgaa tcgctcaacg ggaccgatgg agtatgaatg tacagcacca aatttcgagc
     8821 tgtcagtcag gactatcgct tcgcactctt tttccagcac ggcattgacc acatcggtac
     8881 gcatcatatc gcgaccggtg aatccagttt caggttgcca gccatcaata ccaataaatg
     8941 ctttgctgaa atgcacctgc tggatgcact ggcgtgtcaa cgggccaacc atactttcgc
     9001 tttttttctg gtacacgcca ccgagcaaaa tgacttcaca aggcgcgtct ttcagcaaat
     9061 gcgcgatgta gctgctgacc gtgatgatag tgacattttt cttctgctcc cccagagtcc
     9121 gggcgagcag ggcattgctg ctaccatttt cgataaagat ggtttcgccc ggttgaacca
     9181 gtgacgcggc aaactcggcg agttcacgct tcagcgtata gttgctcatc atacgggttt
     9241 cgacgtcatc actatcaagc gaaactgcaa agccatgtgc acggcggagg taactcagtt
     9301 tttcgagggt gttgagatcc tggcgaatgg taacttcaga aactccagtg gcttttgcca
     9361 gatcggttac gctaacctga ccctggtcaa tgaccatctg tagaatagtt
//
LOCUS       DAPPNJ010000171         9363 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000171 DAPPNJ010000000
VERSION     DAPPNJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.172"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..311
                     /gene="rcsA"
                     /locus_tag="RPM73_003527"
     CDS             <1..311
                     /gene="rcsA"
                     /locus_tag="RPM73_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDX6521849.1"
                     /translation="KSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMW
                     MAGQGTIQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR
                     "
     gene            complement(355..543)
                     /gene="dsrB"
                     /locus_tag="RPM73_003528"
     CDS             complement(355..543)
                     /gene="dsrB"
                     /locus_tag="RPM73_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDX6521850.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            706..933
                     /gene="yodD"
                     /locus_tag="RPM73_003529"
     CDS             706..933
                     /gene="yodD"
                     /locus_tag="RPM73_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDX6521851.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1231..2046
                     /locus_tag="RPM73_003530"
     CDS             1231..2046
                     /locus_tag="RPM73_003530"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDX6521852.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(2043..3737)
                     /gene="yedQ"
                     /locus_tag="RPM73_003531"
     CDS             complement(2043..3737)
                     /gene="yedQ"
                     /locus_tag="RPM73_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDX6521853.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(3975..4157)
                     /locus_tag="RPM73_003532"
     CDS             complement(3975..4157)
                     /locus_tag="RPM73_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDX6521854.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(4236..5153)
                     /locus_tag="RPM73_003533"
     CDS             complement(4236..5153)
                     /locus_tag="RPM73_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDX6521855.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5326..6246
                     /gene="yedA"
                     /locus_tag="RPM73_003534"
     CDS             5326..6246
                     /gene="yedA"
                     /locus_tag="RPM73_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDX6521856.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6235..6705)
                     /gene="vsr"
                     /locus_tag="RPM73_003535"
     CDS             complement(6235..6705)
                     /gene="vsr"
                     /locus_tag="RPM73_003535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDX6521857.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6686..8104)
                     /gene="dcm"
                     /locus_tag="RPM73_003536"
     CDS             complement(6686..8104)
                     /gene="dcm"
                     /locus_tag="RPM73_003536"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDX6521858.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8171..8866)
                     /locus_tag="RPM73_003537"
     CDS             complement(8171..8866)
                     /locus_tag="RPM73_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDX6521859.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(8906..9271)
                     /locus_tag="RPM73_003538"
     CDS             complement(8906..9271)
                     /locus_tag="RPM73_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX6521860.1"
                     /translation="MEKCDFYHIIVLGLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         2273 a         2524 c         2288 g         2278 t
ORIGIN      
        1 ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga
       61 caacgataac ctctttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta
      121 tgttgcgaat gtggatggca ggtcagggaa cgattcaaat ctctgaccaa atgaatatca
      181 aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa acgcataata
      241 aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt atttttgtca
      301 acatgcgcta acacattctg actggtggtt tcccaccagt caggctgaat aagattactc
      361 tgctttctcc acaaagatac cgtcctgatg ccctgcttca ttaaagaacc agatgccgag
      421 cgggtagtct tccagcgaaa ccaggtacat tgtgccttca ctaaactcct caactgccag
      481 taccacgcca ggacgacgcg gaccgccatc cgttttgact gttacccgat cattcacctt
      541 catcgttttc ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc
      601 caggcgcgcg cgagtgctga ttttctcgac ggtctatact taagagatgc caggcggact
      661 taacgactgg cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa
      721 gagtacagcg ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg
      781 atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga acgattctga acacgttagc
      841 gtcgatggac gtgaatatca tacatggcgt gaattggcgg atgccttcga actggatatt
      901 catgacttca gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg
      961 agggggtcgg gatgaaactt gcttaagcaa gaagcactta aaaaattcgt tacaccagga
     1021 aatctgatgt gttcatcacc ttatccgcaa tttttttcgc tgacaagaaa atattcgcga
     1081 tatgaatgat taacgctact tatgaataga aatgtgacgt gcgtcacccc ttggtggctc
     1141 tggcgtcaac gggatcgctc ccgaaaaaat catctttgat tatttgtgct gttttgagaa
     1201 tcatcctggt aaggggagaa ctgcgcgacc atgttttcaa ttcaacaacc actactggtt
     1261 tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca accggctgcc
     1321 ccctggctca gccgtttacg cgaagcgaat gttcccgtca ttctctgtag cagtaaaact
     1381 tcagcggaaa tgctgtactt gcaaaaaacg ttggggctac aagggttacc actgattgcc
     1441 gaaaatggcg cagtgatcca gcttgctgaa caatggcagg atatagacgg ttttccacgc
     1501 atcatctcag gtattagcca tggcgaaatc agccaggttt taaatacgct acgcgagaaa
     1561 gaacatttta aattcacgac tttcgatgat gtcgacgatg caaccatcgc cgaatggacg
     1621 ggattaagcc gtagccaggc ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc
     1681 tggcgcgaca gtgacgagcg tatggcacaa tttaccgctc gtctgaacga actgggctta
     1741 cagtttatgc agggtgcgcg cttctggcac gtactggatg cctctgccgg aaaagatcag
     1801 gctgccaact ggattatcgc gacctatcaa caattgtcag gcaaacgccc aaccacactt
     1861 ggcctgggcg atgggccaaa cgatgcgccc ttactggagg taatggatta cgcggtgatt
     1921 gtgaaagggc taaaccgtga aggggtgcat ctgcatgatg aggatccggc ccgcgtctgg
     1981 cgaacgcagc gtgaaggacc ggaaggctgg ggtgaagggc tggaccattt tttctccgcc
     2041 cgttaagcgt tatcgctcgc gcatacccga ttacgcccgg cctgtttagc gagataaagt
     2101 cgacggtcag ccagtgactg gagttgttca aaatcataat caccggtttc ctcgctgcta
     2161 cttaccccca gcgaggcact gatgcgtatc gtcgtactct tagcaatcaa catctctttt
     2221 tcatttaact tcaggcgaat acgttctgcg acttccgcag cctccgtcag actcgcgcct
     2281 ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag
     2341 gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg atgaccaaag
     2401 cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg
     2461 tgcgtctgat acaatttagc gagcggacgg gctttttcga acagtgcgcc acggttatat
     2521 aaacgcgtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag aacatacatg
     2581 ttgctaacca tccggcgaat cacataccag gagatgagta acatggtggt aaagagcgcc
     2641 cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttaac
     2701 gtatggacgc gcaccagcac accgtcgaaa tggtccagac gttcccagct aacatagcga
     2761 ctgtccatac gaatgccgcc ccgcgtgtca tgttccatcg cctgcgccag caaggccagt
     2821 tcacgaggat caaaaatatt ccctgttgga tggtcaggat tggaagaggt caaaaatctc
     2881 agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc gtttcttaaa
     2941 aattgctgca tggtacgcac gggaatactc atccccagca cgccatacca gtagttatta
     3001 ctgtctactg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc
     3061 gaagtaaacc agcgtaccgc gcggtgacga ttttctcgtt gcgaatggcc gataaaccag
     3121 ggttgggtga catagccgta ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc
     3181 gtcgaaacgt aaaatccggc acgcgagaca tacatcgcct gttcaaccat cgacgaggtg
     3241 ttgtgcgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc attgtcgagg
     3301 ctttcatttt cgcgagacag gagattcccc tcgctgacta atgcatccga tacgccattg
     3361 actgacaggg tgcgtcgtcg gttgagttcg attttccagg cgtgctcgtc gcgatgctgt
     3421 tcgaactcgg ttacagcatc acgcagagag gtgaaatcca gtggcgctac gagagcttcg
     3481 cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg cttatcgagc
     3541 gcgttggcta cgttttccag atgattacgc tggctggaga tataggcatc ttccagcacc
     3601 actacttcac gccaggtgag caaggtggaa aaaagcaata ccacgataaa gcagagatta
     3661 acgacatgac caggccccag gcggcgtgcg agttttttca accagctctg gttttccatt
     3721 tttgtctcgt gctgcaccct gacgactccc aacctgcttt tttatgattc tggcataacc
     3781 tggcgatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat aagacgcgac
     3841 agacatcgat agttttataa cgtaggcatg ataagacgcg atagcgtcgc atcaggcaat
     3901 tcagtgcaga cactggcaca cccgaccgga tgcgacgtga gcgccacatc cggtcgggtg
     3961 ttaatgatac cctttcaaac ttcttccgca ggacgacttc cctcccctgg aacaagctcg
     4021 gtttcatgaa aagcttcccg cttgaccccg taaccgtcat accaacgaca ctcaaccata
     4081 ccgctggagt atccggtgac aatcatccgc gggccgccct ctttaaccgt aacttcctca
     4141 ctaaccataa agctcatact cgcctccttt tttcgagtga aacttgttca ccttagttga
     4201 agatggcgaa ttttgcataa tagccagtgc gagtattaat gtgcctgacc gcgtattttc
     4261 gctacggctt tcactcccag caccacgatg ccgccgatga taaatccaag aatcagattt
     4321 aaaacagtcg gtaatatcat cgccaccact gcactttgct gcccggcgaa atgttcaatg
     4381 gcgtgatgca gcggcgcaat accatgtacc acaatcccac cgccgacgag gaacatcgcc
     4441 agcgtgccga caatcgataa cgctttcatc agccagggcg cgataatcaa taatccttta
     4501 cctaatgcct gcatcagcgc gctggatttt tccgccagcc aataccccag gtcatcaatc
     4561 ttaacgataa cccctaccag accgtagacg cccacggtca ctaccagcgc gatgcctgaa
     4621 agcaccagca cctgattaag caacggcgct tcggccacaa tccccagcgt gatggcgacg
     4681 atttcggcag acaatataaa atcggtacga atcgcccctt ttattttgtc cttttcaaac
     4741 ttcagcggat cctgcgccgc cagcttctcc agacgctgct ggctctgcgc cggatcttct
     4801 ttatgtttac gcgcctccag catatgcagc actttctcta ctccttcaaa gcagagaaac
     4861 gcgccaccaa tcatcaacag aggcgtaatc gcccacggga taaacgcact gatgatcagc
     4921 gccagcggca ccagaatcac tttattaatc agcgatcctt tcgccacgcc ccagaccacg
     4981 ggaagttccc ggttggcccg cacgcctgaa acttgttgcg cattgagcga taagtcatcc
     5041 cctaatacac cggcggtttt cttcgccgcc agtttgccca tcacggagat atcgtccagt
     5101 agtgtggcga tatcatcgag caacgtcagt aaactactcc cggccaaaag cattccctct
     5161 catttttatt gttgaagggt gtaagtatga agcaaaaacg gcagctctga tacttgcttc
     5221 acaggtcaac atttttttaa caattaagtg caattatatc tctcgccagc agaatgattt
     5281 tcacgtttac tatgaaccac ctttttattt ccaccgtgag ggattatgcg tttccgccag
     5341 ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca tttggggctc aacctatttt
     5401 gtcattcgga ttggcgtgga aagctggcct ccgttaatga tggcaggcgt tcgattcctg
     5461 gcagccggta ttttattgat ggcatttttg ctactgcgcg gacacaagct ccccccgcta
     5521 cgtccgctgc tcaatgccgc gctgattggc ctgttattgc tggctgtcgg taatggcatg
     5581 gtgacggttg ccgagcatca aaatgttcct tccggcatcg ccgccgttgt ggttgcaacc
     5641 gtgcccctct ttaccctgtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa
     5701 tgggtgggta tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta
     5761 agcggcaatc cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc
     5821 tcggtttatg gctcgcgcat taccttacct gtagggatga tggcgggtgc tattgagatg
     5881 ctggcggcag gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg
     5941 ctcccttccc tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc
     6001 gccatcaacg cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac
     6061 gcctacgtta acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg
     6121 tcgaagattg aatggctggc gctcggcgta attgtcttcg cggtggtgct ggtcacgttg
     6181 ggaaaatacc tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc
     6241 gagtaaatga atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc
     6301 ttccagacgc tcgctaagcg cttcatccgt cagcttctcg cgcccacgta acgcgcactc
     6361 ccagacaatc aatacgcgcc agccgagttc ctgcaagcga ctgatatcgc ggcgatcgcg
     6421 ctcaacattt ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa
     6481 cagatagcag tgatgatgat gccagaagca gccgtgggta aatatcacgc agcgatattc
     6541 atcaacgaca aaatccgggc gtccgggcag actggcgtcc tgaacgcgaa acgccaggcc
     6601 ctgcccggtt aacagactgg cgaggcgctt ctctatcgcc gtatcacgcg tggcaatcgc
     6661 gcgcatattt ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct
     6721 gacgcaacgc caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga
     6781 ccggcacgac caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc
     6841 ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg cgcgcattca cgaggcgtta
     6901 accgacgtgg acgatgttgc tgattctgcg gatcgtcaaa gtctttctca cccgtggcca
     6961 tatcccagcc acgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg
     7021 tacgggtgac gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc
     7081 gcgcctgatg ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat
     7141 atttcgcctc gaccatcggg tccagcagct gcgccagcgt cactcgctgc gcagggaaac
     7201 attcgctgat atcacgcagg gtaaaatcgg ctttcagatt cagatcgcga cgaaaaccca
     7261 ccagcacgat gcgttcacgg tgctgcggca gaaaatgttt gccgtcgatg attttcggat
     7321 cgtctggccc gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca
     7381 tgatgatgcg gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca
     7441 caaacatcgc cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct
     7501 gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca
     7561 gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac
     7621 gaatatgttc cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc
     7681 ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata atgattggct ttataagtgc
     7741 gtaccgcatg tttgttccat tcgctggtaa acacgcactg tccgccaatc gattcaaaac
     7801 cgcgacggat gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat
     7861 gatgtgccgg tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac
     7921 gatgccatgc cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct
     7981 cacccacacc attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct
     8041 ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct
     8101 gcatagattt aaccggccat ctgagatggc cgacaggtta acataattac agccatttag
     8161 ggccacgcgc ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat
     8221 tctcccccgc cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt
     8281 gctgcgccag ctgcttgcct cttacggttt gcatggtttg cggtagcttc agcaacttag
     8341 tctggaaatg atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgagcggat
     8401 cttcgccatc aaacagcgct acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc
     8461 caatcgcccc cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta
     8521 agggggcgat ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt
     8581 tctccgccgg aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca
     8641 ggattgaaga acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat
     8701 aacaggcggt taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg
     8761 tagcccagac gcggcgaaaa tgacacacgt catgggccgc gtcctggtgc tgatgatggt
     8821 ttttcaacca gttttcgaac tgtgcctgcc agtgttgtaa gtccatacgc gctccgttgc
     8881 caaagagtcg ccaccttagc agtttttatt catagcgtct gctacgtgcg ggacgaggtc
     8941 gggtgtacca ggcaatgctg cctaacaatg ccccgaccag agccataaat aaaaatccga
     9001 ccagtgcccc taaccacttt cccgcggtag agcctaaatc gctttcaaat cccgacacgg
     9061 gggcatcggg gatccggctc atcacccaga tgtaacgcgc cattgcccag acgaaatagc
     9121 cacaccatgt atagaaagcc cacaaagcca gtttgccgcc agggctgcga gagagtctct
     9181 cttccatctt tgtcgaaccg tattccattt tgagataacc cggaaaattc agacctagta
     9241 ctattatgtg ataaaagtca catttttcca cattggatga gccagtcaag actcactcat
     9301 tttttgaaca aatagataat ttttgcgcaa cggcagtgaa tacaggaaaa atcgttttga
     9361 gcg
//
LOCUS       DAPPNJ010000172         9359 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000172 DAPPNJ010000000
VERSION     DAPPNJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.173"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            118..1182
                     /gene="ulaG"
                     /locus_tag="RPM73_003539"
     CDS             118..1182
                     /gene="ulaG"
                     /locus_tag="RPM73_003539"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDX6521861.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1290..2045
                     /gene="ulaR"
                     /locus_tag="RPM73_003540"
     CDS             1290..2045
                     /gene="ulaR"
                     /locus_tag="RPM73_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDX6521862.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2042..2791)
                     /gene="yjfP"
                     /locus_tag="RPM73_003541"
     CDS             complement(2042..2791)
                     /gene="yjfP"
                     /locus_tag="RPM73_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX6521863.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2973..3302
                     /gene="bsmA"
                     /locus_tag="RPM73_003542"
     CDS             2973..3302
                     /gene="bsmA"
                     /locus_tag="RPM73_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDX6521864.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3451..3726
                     /gene="yjfN"
                     /locus_tag="RPM73_003543"
     CDS             3451..3726
                     /gene="yjfN"
                     /locus_tag="RPM73_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDX6521865.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3843..5468)
                     /locus_tag="RPM73_003544"
     CDS             complement(3843..5468)
                     /locus_tag="RPM73_003544"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDX6521866.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5552..6715)
                     /locus_tag="RPM73_003545"
     CDS             complement(5552..6715)
                     /locus_tag="RPM73_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDX6521867.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6718..7356)
                     /locus_tag="RPM73_003546"
     CDS             complement(6718..7356)
                     /locus_tag="RPM73_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDX6521868.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7366..7764)
                     /locus_tag="RPM73_003547"
     CDS             complement(7366..7764)
                     /locus_tag="RPM73_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDX6521869.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7782..8441)
                     /locus_tag="RPM73_003548"
     CDS             complement(7782..8441)
                     /locus_tag="RPM73_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDX6521870.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8504..9190)
                     /locus_tag="RPM73_003549"
     CDS             complement(8504..9190)
                     /locus_tag="RPM73_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDX6521871.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQR"
     gene            complement(9209..>9359)
                     /locus_tag="RPM73_003550"
     CDS             complement(9209..>9359)
                     /locus_tag="RPM73_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDX6521872.1"
                     /translation="GISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEIT
                     EEYSH"
BASE COUNT         2418 a         2473 c         2243 g         2225 t
ORIGIN      
        1 cacttccctg gcaaaaagtc aaaaaatggt cattgccttc ctgaaaaaat agtgtcagga
       61 ttaatcaaaa ccaatcacac attgattcga attgaacaat catgaaggta aatggcgatg
      121 agtaaagtga aaagtatcac ccgtgaatcc tggatcctga gcactttccc ggagtggggt
      181 agctggttga atgaagaaat tgaacaagaa caggtcgctc caggcacatt tgcgatgtgg
      241 tggcttggct gcaccgggat ctggttgaaa tcggaaggtg gcaccaacgt ttgcgttgat
      301 ttctggtgcg gcactggcaa acaaagtcac ggtaacccgt taatgaaaca gggtcaccag
      361 atgcagcgca tggctggcgt gaaaaaactg cagccaaacc tgcgcaccac cccgtttgtt
      421 cttgatccgt ttgcgattcg ccagatcgac gcggtactgg cgactcacga tcacaacgat
      481 catatcgacg ttaacgttgc tgctgccgtg atgcagaact gtgctgatga cgtaccgttc
      541 attggaccga aaacctgtgt ggatttgtgg attggctggg gcgtaccgaa agagcgttgc
      601 atcgtggtca aaccgggcga tgtagtaaaa gtgaaagaca ttgaaattca tgcgcttgac
      661 gctttcgacc gtactgcact gatcaccctg cctgccgatc aaaaagcggc tggcgtactg
      721 ccagatggca tggacgatcg cgcagtaaac tacctgttca aaacccctgg cggttcccta
      781 tatcacagtg gcgattccca ctactccaac tattatgcga agcacggtaa cgaacatcag
      841 atcgatgtgg cgttaggatc gtatggtgag aacccgcgcg gtattaccga caaaatgacc
      901 agcgccgata tgctgcgtat gggtgaagcc ctgaatgcga aagtggttat cccgttccac
      961 cacgacatct ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct gtgggagatg
     1021 aaaaaagatc gtctgaagta tggcttcaag ccgtttatct ggcaggttgg cggcaaattt
     1081 acctggccgc tggataaaga caacttcgag taccactatc cgcgcggttt cgatgattgc
     1141 ttcactattg aaccggatct gccgttcaag tcattcctgt aatctgtcgt ttataccgga
     1201 tagcgtggag aagttcgcgc tatccggcgt agtatttcct ggctatttca aatatcatct
     1261 aaaaaaatca aattttatcg gaatagctca tgactgaagc acaaagacat caaatcctcc
     1321 tggaaatgct cgcacaattg ggctttgtga ccgttgagaa agtcgttgag cgtctgggaa
     1381 tttcgcctgc cactgcgcga cgcgatatca ataaacttga cgaaagcggc aaactgaaaa
     1441 aagtgcgcaa tggcgcagaa gctattaccc aacagcgccc gcgctggacg ccgatgaatc
     1501 tgcatcaggc gcagaatcac gatgaaaaag tacgtatcgc taaagcggcc tcgcagctgg
     1561 ttaatccggg cgaaagcgta gtcatcaact gcggctccac cgcgtttctg cttgggcggg
     1621 aaatgtgtgg caagccagtg caaatcatca ctaattatct accgctggca aattacctga
     1681 tcgatcaaga acatgacagc gtgatcatta tgggcggaca gtacaacaaa agtcagtcca
     1741 tcactttaag cccgcagggc agcgaaaaca gtctctatgc cgggcactgg atgtttacca
     1801 gcggcaaagg actgacagca gaagggttgt ataaaaccga tatgctgacg gcaatggcag
     1861 agcagaagat gctgagcgtg gtagggaaac tggtggtact ggttgatagc agtaagattg
     1921 gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat tgatatgctg atcaccggca
     1981 aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca aggggtcagc attctgcgtg
     2041 tttaaagatg ctggcggaaa aatgtcactg cagcatctaa cgcctcaggc gtaatgcggt
     2101 gacgcacgcc tggctgccat gaacaggtta gcagtttatc ccgtcccgtc tcgcttaagg
     2161 cctgctgcaa acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg ccatgccaca
     2221 gcagtagagg tctgtcacta agttgttcca ggtggtttgt cgcttcccac tctgccagcg
     2281 gcgcgacaat gttattgaat tcattctgct gtgctgtcgt ttcaggtatc agcggtggaa
     2341 acagtgaacg ggcgagtgat gtaaaatagc ccgatcccat catgctggcg gtacatctca
     2401 ccgtggggtg gcgagcggta atccccagtg ccgtcatcgc gcccatcgac gcaccaccga
     2461 ctgccagacg gtcatcaagc agccagtttt cttcggctat tgccgcacgt aaagtagtga
     2521 attcctgcat actttgtagc aagatttgcc agaattgatt taaccgccgc gctgcgtcac
     2581 cactaaaacg gctaccgtga tcgggcgcat ccggcatgat cactcgcaaa ccagcctgcg
     2641 ccagcgcaac ggcaaaatag ctatacacca gactggatga agtaaagccg tgataaaaaa
     2701 ttacgcacgg taacggggta tctttttgcc cgacaggata agcatgaaga acgggaatat
     2761 ctgccagctc gcgtgattct atttcaatca tctgtctctc ctttttgtct ggtatgacat
     2821 gaagaaatcc ttagcacaag tttctgctgg cggtattagt tcataatgtt gagatgtggg
     2881 ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt cgggaacatt
     2941 cccccaaagt taaattaata aggcactaca ctatggttag caggaaacgt aatagcgtta
     3001 tttaccggtt tgccagttta ttattggtgt tgatgttaag tgcctgtagc gcactgcaag
     3061 gtacgccaca gccagcaccg ccagtaacgg accatccgca agagattcgc cgcgaccaga
     3121 cgcaagggtt acagcgaata ggcagcgtaa gcacgatggt tcggggttct ccggatgacg
     3181 cattagcaga aatcagagcg aaagctgtcg ctgcaaaagc tgattattac gttgtcgtca
     3241 tggtggacga aaccatcgtg acaggacagt ggtattcaca agccattttg taccgtaaat
     3301 aaatcagaca acttacatag tcagctttac attgctttgc gtccctatgc gtttacctgt
     3361 gcacaataaa ttaccggctg gaagcctggg cgtttattac cgcgatggaa tgccctgcaa
     3421 catgtgggga aacgaaaaat ggagctgacg atgaaacaat tacttgcctc accctcgctg
     3481 caattagtga cttatcctgc gagcgccacg gcacagtctg cagaattcgc cagtgctgac
     3541 tgcgtgacgg gcctgaatga aataggtcag atctcagtta gcaatatttc aggggatccg
     3601 caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc atggtaccgc
     3661 attattacca tgtacgaaga ccagcagcct gataactggc gcgtacaggc gatactgtat
     3721 gcatgagtgt catctacagc aaatgaacaa tgccggatgc ggcgcgagcg ccttgtccgg
     3781 cctacaattt tcacatattt catttagtta atcgaaacca gcgtcgcatc agtcgtatac
     3841 gcttacacac acactccccc cgtcgcccgc agcaataaat cattctggat ctgctctgac
     3901 aaccgtacgc cgccgcgcgt atctaacatc acctgacacc acgcctgcgc cattggcgga
     3961 gaagcatatt tcaacatttg cgcaccgcag cccagcagga atagctgatg agtaatctct
     4021 cgccccaatt cttcagctgg cttgcgcagc tgctgctgta aacgacgaac cgcgcgatca
     4081 aaatagcgat cctgcccttt cacttccaca aacgcttccg acaataagtc gtatacgccc
     4141 gcttgcttat tgagaacgcg cagcacatcc aggcacataa tattgccgga accttcccaa
     4201 atactgttta ccggcatctc ccggtaaagc cgcggcagct cgctctcctc gcaataacca
     4261 atgccgccca gcacctccat cgcttcggcc acaaacggca tacctctttt gcagatcaca
     4321 aattttgccg caggcgtaaa taaacgcgcc cacagggctt ctttggcatc ggcacgccgg
     4381 tcccacgctc gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg aagtgccatg
     4441 cgacttaaga catgacgcat aaggggctgt tggatcaatg gattaccaaa aacatggcgt
     4501 tgatgtgcat gataaatcgc cagcgaaaat gcacggcgca tcatggcatg gctacccagg
     4561 gcgcaatcaa aacgcgtcat cccacccatt ttcaggatca gacgaattcc ttccccttcc
     4621 tgccccaaca accagccaat ggcatcctga aactccactt cgcagctggc gttagagcga
     4681 ttacccagct tatctttcag ccgctcgagg cgaatcgcgt tgcgttgccc gtcaggcaaa
     4741 aagcgcggca caaaaaagca ggacaaaccg cccgtagtct gcgccagcac cagatgcgca
     4801 tcgctttgcg gcaccgagaa aaaccattta tgccccacca gccgataaga gccatcttcc
     4861 aggcgctctg cgcgggtggt gttgctcatg acatcggaac cgccctgctt ttccgtcatt
     4921 cccatgccaa tcaacaaacc gcgtttttgc ccacctggca ataagtgaga atcgtagcga
     4981 tcgctcagca gcggcgtggt ccagtcctga aacggcgcgg gtaacatctg taacagcaat
     5041 ggcgtggcgg caaaggtcat ggttatcgga cataacgacc ctgcctcaac ctgtgcgtgc
     5101 aacataaaac gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc ttcccaggca
     5161 agattgtgca cccgattggt acatagcgcc tgcatcagca ggtgccaggc ggggtgaaaa
     5221 cgcacatcgt ccagacggcg tccttgcgca tcgtagcgca ataattcagg cggattcaca
     5281 ttcgccagcc gccccagttc aagggattca gccgttccta actgctgacc aatactggct
     5341 agaaaatcgc tatcccagcc agcaccttca cgcgttaccg cttcgcagag cgcgccatca
     5401 gacaggtata agttactgtt atttaatggt ataggttgat taaaaacggt gtgagtttgc
     5461 cagtgcactg tgtctccctc catcaatggc agtcaccatt agtatggtca ctgccatgga
     5521 ttcatgacag aaagagattg ctaaaacatt cttatccagc aatgtaatgc ggaacgaaac
     5581 gtgaggtgtc tttggtgatc agtgtgttat cttcacggat ccccattccg cacgcttcat
     5641 catcgacaat ccagctaccg atgagtgtgt agctatcgcc aaatcgcggc agaggttgaa
     5701 acgcctggta gatcatcggt tcatcggcgt aatcaccatc agcgtggtca acgacgttat
     5761 tctgaccgtc gaaaatggtg acgtttccgc cttcgcgcga gtagatcggt ttacgcacat
     5821 agctttcgcc agcggcaatc tgcgattttt caccctcaaa ccaggatgca agaaggttag
     5881 gatgaccagg gaagaagcgc caaagcagcg gcattagccc tttattactc aagatacttt
     5941 tccataacgg ctccacccat tgctcgcgac gcttgcacag cagcggaccg ttatcgtcac
     6001 gcatcatcca ctccagcgga tacagcttaa atgcacgctg gatgacatta tcatcaagat
     6061 cggtcagtac accgccgacg cccaaaccga gatcttcaat gtagataaac cgcgactcct
     6121 gccctgcctg ctgggcgcag tcctgcaagt acagcacggt agtcctgtct tcatcggtgt
     6181 cctgacagca gcagaaataa aacggctccc ggctataaag ctcgctaaag cgtgaaatca
     6241 ggcgttcctg aatggcattg tactgatcgg catcacgcgg aataatgccg ctgcgccggg
     6301 catcctccag ccacaaccac tggaaataag ccgactcata caacgaggtc ggcgtatcgg
     6361 cgttgtactc cagcagcttc accggcgcat tgccacacca ggcaaaatcc atccggccat
     6421 acagcgaagg atcgcgtgcg cgccagcttt cggcgatcac atcccagtac aacggcggga
     6481 tcgccagttg cgtcaggatc tcttcgtctt taaccgcgcg atccaccacc tcaaggcaca
     6541 tctgatgcag ttccgcagtc ggtttttcga tctgctcttc aatctggcgc agagtaaaac
     6601 ggtaagcccg actctcgtcc caatagattt cattgtcgat gatatgaaag tcgaaaccgt
     6661 tgttagcggc gatctggtcc agatcccgtc gcacaggaac gttgtgtctc agcatgatta
     6721 gcctccccaa tgcccacggg cgctggaaga acgaccatag ccgccgcgcg aaacggttga
     6781 cgcttttttg gtggtaaagc cgcctgaaga gtaagactct tttttgccag aaccggagcg
     6841 ccaggagtaa tcaccggaag tgttgcgcca gacggggcgg gaagcaaatg aggatccgcc
     6901 gctgttatag acgaacggct catcgcgatc tttacgaata actcggctta acaaaaatcc
     6961 agaaacgacc gggatccaac tctgttcaac attgtcgtag tagcaatttt cgtacttaga
     7021 ctgacaattc tgctgagtca tattcttcgg aacatcggca taaaatgccg ctttggcgtt
     7081 gttccagcca cgcgcgcaaa tgtcggcatt attaccgtca tcaatacaat cctgcactgt
     7141 cgaataaaac gttccgtcgc cgtcgttatc gacatcgcca ctttcactcc atcctttgac
     7201 tattaaaaaa acggcaccgc ccattagcac caacgttaag tatttttgtg cgtagcgatt
     7261 gcggcgcggc tcaaaaggat tatgcggtct tccaatgcga ctgatcgctc cgtgaccaat
     7321 tttactgtta tttcttgatt tgcgttttct ggccatgatc gtccattacc atgtcataca
     7381 agctgcgtta aaaataccac cagccagtgc ggcggttccc ataaacattc ctgctgcggt
     7441 attgtgatta ataatttttt cgcttaatgc gggcatataa agtctgacac cagcaaaaac
     7501 gagtaactga atcaccagtg caatcccgcc ccaggcgaaa tagtctggaa tactcaccgc
     7561 attgattgcc gcactggata aggggatcac gtaacccaac aatgtaccgc tgaatgccag
     7621 tgacgccgcg gtattattgt ttttgattaa ttgccattcg ttgtgcggtg taattttgga
     7681 gtagataaac agaaaaataa tcaccatagc cacgccaata aaaaaatagg cactaaaggc
     7741 aagaagtgaa tccagtatat gcattgtaaa cgtccttgta attaaccaat aatatgaagt
     7801 gatgtcagcg ggatatcgac acccaacgcg cgcgaaaata accactctgg ctcgccgaga
     7861 tcgttaaaag attcctcacc atttaacagc aaatattcgt aagtatcttc ggttacttgg
     7921 cgttggtagc ccatggtaaa attatggacc tcccatttgg catggttttg attttctact
     7981 ttttccagca tgtagacggg ttcgatattt cccggttctt cgctgttaaa aaatcgctgc
     8041 cagcgttttt cctgccagtt taagatcatt gcccccatca ctttggcgtt gatcgcctcg
     8101 cgccagtgac tctctttact gataccgtag ctctcttcat agacaaagag cttgatatca
     8161 tcaatgtcat caatatcttc gccgccggtg gtattgattt gcagaaattc atcgcctgaa
     8221 gtgtagtagc ggaaaatctg actaccgccg cccagatcga tgtggctgac agcggctacc
     8281 gtaaattcct cacccggcag cgcaatcagc aattcatctt ccagcaaacg aaacgctaac
     8341 gtgtcgagcg taaagccact attgagatga agtcccagcg gaccgcgagc gatagcaggc
     8401 ttattatcct tgccaaacag acgctggaaa aaaccagaca tattttatcc ttaaaatagt
     8461 tgccagcctt ttagcgaggc tggcaaaaaa attactcgcc ctgttagcgt tgcagtcttg
     8521 ctaatacatc ctgggcgcta cttttattgc taccgccaat tccggcttcc gccagctttt
     8581 catcaagatc gcgaccgtct gcgacttttt ccaactgtgc agcagcatcc aggcgagcct
     8641 gacgttcggc ctgacgcgtt tgcaggcgtt ttaaggattc tgccgctgtc gaaacgctgg
     8701 aagatgcgcc aacggtagag gttgttacag cctgttgtgc acgctgcatg gcttcagtgg
     8761 ctttaacgac ttccatttgc tgctcaaact gagcgatacg ctgcgctgtc gctgtaactg
     8821 ctttttccac gccatcacgg gagacttcca gattcgacaa cacttgctct tcagcggtaa
     8881 tgagattctc aaggcgcgcg atttcttcag caacttcgtt aatcaacgac ggattaacgt
     8941 tcttgcttaa cgcttccagc gcacgggctt ccagactggc tttgcgctca cgtaaatctt
     9001 tcagcttatc gtgacttaat ttcacccgcg ccagcagatc aacgcgagat tttccggctt
     9061 tatcgagttc agctttagcg tcacgaatat gctgttccag catgcgcacg ccctgggttt
     9121 cttcaatgga ttcttccgcc tgggagataa acgatttccc cagcgtaaat aaacttttta
     9181 aaattcccat aaatacccgt ccttgtaatt agtgtgaata ttcttctgta atttcagcca
     9241 gatccaatgc gttatctacc agcgaggtta tctcaagcag gatatcttca agagaagatt
     9301 taagcgataa cgccccgaaa acaatgtaat actcttcctg ttgcacgctg gagattcct
//
LOCUS       DAPPNJ010000173         9348 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000173 DAPPNJ010000000
VERSION     DAPPNJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.174"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..739)
                     /locus_tag="RPM73_003551"
     CDS             complement(<1..739)
                     /locus_tag="RPM73_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDX6521873.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            744..819
                     /locus_tag="RPM73_003552"
     tRNA            744..819
                     /locus_tag="RPM73_003552"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:777..779,aa:Asn,seq:gtt)
     gene            complement(857..1807)
                     /gene="cbl"
                     /locus_tag="RPM73_003553"
     CDS             complement(857..1807)
                     /gene="cbl"
                     /locus_tag="RPM73_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDX6521874.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1909..2826)
                     /gene="nac"
                     /locus_tag="RPM73_003554"
     CDS             complement(1909..2826)
                     /gene="nac"
                     /locus_tag="RPM73_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDX6521875.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3153..3228
                     /locus_tag="RPM73_003555"
     tRNA            3153..3228
                     /locus_tag="RPM73_003555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3186..3188,aa:Asn,seq:gtt)
     gene            complement(3284..4219)
                     /gene="ldtA"
                     /locus_tag="RPM73_003556"
     CDS             complement(3284..4219)
                     /gene="ldtA"
                     /locus_tag="RPM73_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX6521876.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4281..5360)
                     /gene="cobT"
                     /locus_tag="RPM73_003557"
     CDS             complement(4281..5360)
                     /gene="cobT"
                     /locus_tag="RPM73_003557"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDX6521877.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKNAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5372..6115)
                     /gene="cobS"
                     /locus_tag="RPM73_003558"
     CDS             complement(5372..6115)
                     /gene="cobS"
                     /locus_tag="RPM73_003558"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDX6521878.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6112..6657)
                     /gene="cobU"
                     /locus_tag="RPM73_003559"
     CDS             complement(6112..6657)
                     /gene="cobU"
                     /locus_tag="RPM73_003559"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDX6521879.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHVDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6881..7015
                     /locus_tag="RPM73_003560"
     CDS             6881..7015
                     /locus_tag="RPM73_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HDX6521880.1"
                     /translation="MEQISLVKCWLREAGAHNVLVTSAVNNNRVAELFALLHIEEVCR
                     "
     gene            complement(7116..7445)
                     /locus_tag="RPM73_003561"
     CDS             complement(7116..7445)
                     /locus_tag="RPM73_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDX6521881.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7617..8675)
                     /locus_tag="RPM73_003562"
     CDS             complement(7617..8675)
                     /locus_tag="RPM73_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX6521882.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(8873..9346)
                     /gene="sbmC"
                     /locus_tag="RPM73_003563"
     CDS             complement(8873..9346)
                     /gene="sbmC"
                     /locus_tag="RPM73_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDX6521883.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         2507 a         2483 c         2079 g         2279 t
ORIGIN      
        1 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaatc aaagtgatgg
       61 cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt
      121 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaataagcg
      181 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
      241 ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga
      301 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca
      361 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacacccatc aacaggacac
      421 aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
      481 tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
      541 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag
      601 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
      661 atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga tttttcgccg
      721 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
      781 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
      841 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
      901 gccgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccagacat
      961 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     1021 gccgcgtatc cagacggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     1081 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac
     1141 ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc
     1201 ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg
     1261 gtgaaatttg cgtcaaggga tgatcgtgtg gaacaagcaa actatggtgc caacgaaacc
     1321 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     1381 cttcgccatt ttgcaacaat gtcgcaattt cctgtggcgt cccctggatt agctcgagcc
     1441 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     1501 gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa
     1561 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     1621 ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     1681 catcctccag ttcccgaata tgacggctga cgcctgactg tgaggtaaaa agcatattcg
     1741 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     1801 aattcacggt aaactccggg cagttcagat ttcccgttat tgttaaagtc taatgcccgg
     1861 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacactt agctcaccaa
     1921 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actctttcac
     1981 tgcctgtgcc tgtggcgata agttcgctct ggcgggtaaa tttaatgaca aagagagact
     2041 catggaagga gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc
     2101 ggccgattcg ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtggc
     2161 aatagactca atttcaccaa taacttttgc cgtgagtcgc cgtagggaaa aagcctcatc
     2221 aacacgaagt ctaatagcac tgtaatcact ggggagaaag aggttcattt tcgcaatagc
     2281 attcacatca acgctttgcc ccgggcaatc ttgagttcct accagaaaaa gatcttcttt
     2341 cagcaaagcc tgactggata caccagccac aggggaatgc tcataaatca ccgccatatc
     2401 gagttggtga tttatcaatt tttcgttaag cactgcacca ctattttcat gaagatagat
     2461 aacgatctcc ggaaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc
     2521 agcggttcct ggtgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg
     2581 caccgccagt tgggcctgtt cacactgacg taaaatggcc cgcgcatggg tatagagaat
     2641 ttttccggcg tctgttggcg taacgccccg ctttgtacgg atcaaaagtt gttgatttaa
     2701 ctcaccttcc agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc
     2761 agcagcctgg gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa
     2821 gttcatgttg cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccaga
     2881 ttgcaagatg cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat
     2941 aagagattat gatagattcg tcatttgctc ctttaatcag ctgtcgcgtt cccctgccct
     3001 ataaaaggag ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact
     3061 ttgttgaaat tgacagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc
     3121 actgccatta atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc
     3181 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag
     3241 cctgctttct agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg
     3301 aaccagaact aatttgcaca ggcattcccg atcgacgttg caacgcagca tttgcgcgat
     3361 ttacatcaac ttcttgcccg ctgataaacg cccgcaaaga tggggttacc ggcaatggca
     3421 cttttcggtc agactcatat tctgcacgat tgcgcgacaa cggctcatga acttccagcc
     3481 agttcgagcc atctggttca gtggtgtatt ttactggctg gtcgataatt tgcacacgcg
     3541 tcccaacagg aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc
     3601 cctgacttac ccggagccca ataccaaaat tggcattggt accatggatg gcatacaacc
     3661 tgccaatata aatcgcgtac agccccatgg gattatcggg gcccgcagga acaaatgcgg
     3721 gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg
     3781 gagcttcttg tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct
     3841 ggccgatacc aataggaaag acttccacag tattactgtc tggtgggtag taataaagac
     3901 gcatctcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct
     3961 gcggaatggt gagttgcgag ccagacttcg gcaaaaaaac atcagcgccc gggttcgctt
     4021 ccagcatgtt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgggtat
     4081 tgtgatcagg aacagttaca gtaaacgact gccccactaa acggctaccc tctggaggta
     4141 atggataagt taccgccagg ctagtatggc tggcaaaaag tagagcaaat gagcaaaaaa
     4201 tatttacacg acgcatcatg tccctttcct aagtcgcgaa agctatccgt taagtatagc
     4261 ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca
     4321 atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg
     4381 ggcatcgcca gagctgcgcc actcccctca cctaaacgca tctccatatt gagataaggc
     4441 tccagcccca aatgcgacag cgcaatacgc gccccttttt ctgccgacag gtgagaagga
     4501 atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcggc agcataagaa
     4561 agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat cacgccagcc
     4621 attccgacca aatcaaatcc acccactttt gccaggacat caacgccatc ctgcggattt
     4681 ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc
     4741 ggcaggtttg cgccaatccc aaccacttct tcaggagccc ggccagtgat tgtgctgact
     4801 attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtaaca
     4861 ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt ttctgcctga
     4921 tggcgactca ttgccggagc tgaagcaata ttgccactac ctcgagcaac acgcatgttg
     4981 ataagcccgg ggataggctc agcagtatca ataccaacat caattacgta gacgttagcg
     5041 cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga
     5101 atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc gtgatcggca
     5161 cacataacca gtaccgcgtt tttgcccaca tgcggtatgc cattcaaccc cggcattccc
     5221 gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg
     5281 tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga tagcagggat cgtattcagt
     5341 aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg aagaccagtt
     5401 caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga
     5461 gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca
     5521 tacccggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc
     5581 gcccatcaat tttgccaatg aacacattgc caagtccttc ttcccgggca taacgatgac
     5641 gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa
     5701 ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc agtacaacaa
     5761 aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc
     5821 gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccaggtgga
     5881 atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac
     5941 accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta
     6001 aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac
     6061 gcggtacagg cagacgcgta ataaaagaga gcatcgccca aaataattta ctcatttgat
     6121 ttttactcca atacccgaaa ccaccaacca tacttcattt gccgcagcgg ccagctgctg
     6181 atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc
     6241 cattcccact tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat
     6301 caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg
     6361 gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag
     6421 caacacagct tcatgcgggt taatgtctgc atgaattaat tcatcaacat gttgccaacg
     6481 ctccaccgtg cgccagtgcg ccggacggcc ttgccgatga tgttctatcc gtgcagccat
     6541 ctcatcatca aggatttgcg aggtggcgat atacagaacc tgtgaagagt ccccaataag
     6601 cgcctctgcg tggcgactct tcccgctccg tgctccacct gtcaccaaaa tcatcatacc
     6661 gactcctgat tcaattgtat gatggtattc attgaacaga acaatggatc ccccaccgtt
     6721 gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat
     6781 tctcaatcca ttacgaaaga agatggcatt gtggaatagc cagacttcct cgtgttaacg
     6841 cccggtcatt ggagtagtaa ccaaagcaga tttagccaac atggaacaaa tatctttggt
     6901 gaaatgttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cggttaataa
     6961 caatagagtt gcagaacttt ttgccttgct gcatatagaa gaagtctgtc gctaatttcg
     7021 acaggttatc agatgggaat gatgactttg atacacagaa aagaatcaat gagcaatgag
     7081 ggttgccggg caaccctcat tgaataaaac aggaattact tcgcttcgcc gtttttcatt
     7141 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
     7201 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
     7261 tggattgctt caacgctcat ccccggcttg atgtaatcgc tcaggttgtc cagcagcagg
     7321 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     7381 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     7441 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     7501 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     7561 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     7621 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgcgc cgtttccatg
     7681 ttcagccaca cataaccata gattggtgtt tccactacct tcaggtcatg gtggttattg
     7741 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     7801 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     7861 tgggtatcac gaagtttttg cgcgttcaat aacacgaaat ggctggggcg cgtggcccag
     7921 tacgcattga tttgcaactc cagcatacaa accagattgc ggttaatggt ctggatacct
     7981 tcatatatcg attttggaat acgggtttct ttgctggcgg gcgcaatcag cccacgcatt
     8041 ttcacggcat cggtcaggag tttttgtaga tggctttcca gacgtgggcg ttcgagtaag
     8101 ttcggtgaga atgcagattg atagacccga ttatactcgg tcagactttt cgccagttga
     8161 atgcgccagt ggatgaacgc ccgttgtggc cagataccgg taaacaacat tgccagtaaa
     8221 gagccgagga tcacatcgcc gcttcgccat aacgccgtat caatttcacc tgtcggggaa
     8281 ccaacaacaa ttgccagcgt caccccaatc aataatcctt gatacggttt cttgcccagc
     8341 gccagccaac cgcaaagaaa catcgccgcc gcgcaccaga ctaacatcag cggtaacgaa
     8401 attaactcca gttgcagagc gataagacct aaaatcgaac ccaacaccgt accgccaata
     8461 cgttcgaagg cgcgagggac aacgttaccc cagaacgaga ttggccccat aatcaccacc
     8521 atggtgacca gcggccaggt gctttccggg atagtaaaca ggcggataat gagaaaagtg
     8581 agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg gtatacccgg
     8641 atttcaaacg ggcttaatga cttatcggca cgcacgcaga aactccgtaa tgaagattaa
     8701 aagcgctcat aactcatctg tcggccagaa ggcgcacctc tgagttatcc gacggacata
     8761 ccgggaacag tgtaatttcc cggcaggagc gaaaaatttg aaaggcgcat cttactcttt
     8821 tcgctttcaa aaaaagagtg gtcttcgcgt caacacaccg ccctgagatg aattagtgat
     8881 gttttggctg caccgcaaca tacatttcga tatcccagta cccatcttcc gcgccattgt
     8941 tcaagtaaac ctcgaagcag ggctttggta acatttcata agcactgtcc tgcaagagac
     9001 tattaaagaa ctgataccag ggtttagcaa aatcatcacc gactacacga gctaccgcca
     9061 ccgcatactg accacctgta atttctgtca gaatgacgcc ctcactgttt tcgggaagcg
     9121 taaagtaacc cggcaccgtc acgacggtgt cgcagcgtaa tttttcggcg ggtgtttcat
     9181 ctggattgtc gtaatagaca gcaacccact ccttcggcac aatatttttg ttatctaccc
     9241 acatcatcaa ctgctcaaag cctttcttta ccgtctgttc ccacgggcca acgagatgga
     9301 aacctgcaac ggtacgtttc tcttcctgct taatctcgta gttcatga
//
LOCUS       DAPPNJ010000174         8964 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000174 DAPPNJ010000000
VERSION     DAPPNJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.175"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            96..368
                     /locus_tag="RPM73_003564"
     CDS             96..368
                     /locus_tag="RPM73_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDX6521884.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(369..1241)
                     /gene="rluF"
                     /locus_tag="RPM73_003565"
     CDS             complement(369..1241)
                     /gene="rluF"
                     /locus_tag="RPM73_003565"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDX6521885.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1453..2142
                     /gene="pepE"
                     /locus_tag="RPM73_003566"
     CDS             1453..2142
                     /gene="pepE"
                     /locus_tag="RPM73_003566"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDX6521886.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2233..3864)
                     /locus_tag="RPM73_003567"
     CDS             complement(2233..3864)
                     /locus_tag="RPM73_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDX6521887.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4084..7767)
                     /gene="metH"
                     /locus_tag="RPM73_003568"
     CDS             complement(4084..7767)
                     /gene="metH"
                     /locus_tag="RPM73_003568"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDX6521888.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            7967..8791
                     /gene="iclR"
                     /locus_tag="RPM73_003569"
     CDS             7967..8791
                     /gene="iclR"
                     /locus_tag="RPM73_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDX6521889.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2069 a         2473 c         2313 g         2109 t
ORIGIN      
        1 ataaatattt aagaagagaa caaaggaata ttatcagttc agctcgcaaa cgaattcccg
       61 tacactgaac tctctggctc actgagggca ccatcatggc actcccccgt attacccaaa
      121 aagagatgac tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg
      181 cgcatggtcg cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata
      241 aattaatggt cgagcgtgag gctgaagcga aaaaagcccg ccagttgaag aaaaagcagg
      301 cttataaacc ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca
      361 ggcgctgatt agcgcccctt ctttttacgc cccggagagg taaaacgctt accgttggac
      421 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
      481 gctgttttcg gtttggcctt cactttaggt tttgcctcgg aagaggaatt ttcgatgagc
      541 ttaaagaggt cgattaactc atcatcagtt aaatcgcgcc actcacccag cggaatgccg
      601 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
      661 tcgcacatgc gccggatctg acggttcagc ccctgcacca gggtaatgcg gaagacaaac
      721 ggcgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cacccccgcc
      781 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
      841 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
      901 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
      961 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
     1021 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
     1081 actttcacaa cgtcgccggg tttcacctga tcgccaatgg tggctcgctt gccattaagg
     1141 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
     1201 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaaaac
     1261 ggaatatacc gtacctttgg gttgataaaa aatagtcatg agcgaactat ttgttatttc
     1321 cattggcttg aattcgttac ctgctcaaat cactttccag aagcaatgtg cgacatcgtg
     1381 agccatgtca gtaagtggtt aaattcgcag tggtagcatg cctcccgtga tttcttacta
     1441 accggagaag taatggaact gcttttattg agtaactcga cgctgccggg taaagcctgg
     1501 ctggaacatg cactgccgct aattgctgaa cagttgcagg gtcgccgctc agcggtgttt
     1561 atccctttcg ctggcgtaac gcagacctgg gatgattaca cagcgaaaac ggctgcggtt
     1621 ctcgctccgc tgggtgtttc tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg
     1681 attgaaaatg ctgagatcgt gattgtcggc ggcgggaata ctttccagtt gctgaaacag
     1741 tgccgcgagc gcgggctgct ggcaccaatt actgacgtgg ttaaacgtgg cgctctgtat
     1801 attggctgga gcgcaggcgc taaccttgct tgcccaacca ttcgtaccac caacgatatg
     1861 ccgattgtcg atccgcaagg tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg
     1921 cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc
     1981 gaactgctgg tcgtcgcgcc agaactgacg attattggtc taccggaagg taactggatc
     2041 acagtgagta aaggtcacgc tacgctgggt ggcccgaaca ccacttatgt gtttaaggct
     2101 ggtgaagaag cggtgccgct agaagctggt caccgttttt aagtgaattg ccggatgcat
     2161 gatccggcat cttgttcata gtgcctgatg cgacgcttga cgcgtcttat caggcctaca
     2221 ggattcacaa tattaatact cgtcccgtcc ttcatcttca tccggctgtt ccagcacact
     2281 gtaagccacc gaacaaaaca gcgagttcag gcgctgcata tctcccagta agcctaaatg
     2341 tagcgaactg gtttcaatgc tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg
     2401 ggaatagcgg cgattaagaa tgcgaaagcg atgcttacta cggcgcaaac gacgagcgct
     2461 ggtgacatcg ccagagaaga acaccgacat tgccagcttt aaattactga gcaattgctc
     2521 atagagcgca tccagttcct tcaacccatc aagcgaaaat gcccgccgtg ctgccagtga
     2581 tttgtcggca atttcactcc ccatgcgctc gacgatatcg gaggcctgtt caaggttgag
     2641 cgacatttcg atgatctctg cccagcggcg cgactcttcc tccgccagct cttcttttgg
     2701 catccgcgcc agatacagct taatggcggt atagagaacg ttgatatcat ctgccagctt
     2761 acgcagctct ttctcctgcc gtggctcgcc gtgcatcact ttattcagcc cttccatcat
     2821 ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt
     2881 gggcgtatcc agcgcgctga catccagatg tttaggccgt agctgggtat ccagttccgg
     2941 ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac
     3001 caggcaacgt acaaggttgt agaagacgtg gaaatagatc accagttccg cttttggcaa
     3061 tgacaacttc cccattgtct ctgccagcaa atggacaaac ggcaggataa tcaggctacc
     3121 caccagctta aacagcaaac tacccagcgc gacacggcgg gctgcggcat tggcggcact
     3181 gttgttgagc atcgccagca ggccggaacc gaggttagca ccaatcacca gacagagcgc
     3241 cacggggaag gagataatgc ctgcggcggt cagagtcgca gtcagcagta cagcagcaag
     3301 gctggagtag ctgataatgg cgaacatcgc gccaatcagc gcatccagca gaatatcgcc
     3361 ggtcagcgag gcaaagatca cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg
     3421 cacaatcaac tccagcgcca gcaaaatcag cccaagacca ataccgacgc ggcccagttg
     3481 cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg
     3541 tgacagccag gataagtcga aggtgagaat acgcgccatt agcgccgtcc cgacatctgc
     3601 acccagcaca atcaccagag ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt
     3661 caccagcatg gtggtggcat tactgctctg caccagtgcg gtaacgccga tccccgcgca
     3721 aaaggcgagc ggcttttttt cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa
     3781 gacgcgcatt acgccggttc gaacaatatg agtcccccag accagcaggg cgacggcaga
     3841 aagcaggtga agcagcgtta acacgagttc aggttctcct tatcgttata tgtgcctgaa
     3901 gcaattgaat gaccaaagga ccggaaacac gctgactggc atctttactg tgccttttga
     3961 cattcgtttt tccggctaag attattccag cctgtttcca gtataagtgt ttcatattaa
     4021 taaagagaca gggcactcat tgagcgccct gtttgttgta aggaaaagtg acagatttgt
     4081 gaatcagtcc gcgtcatacc ccagattcgg tgccagccag cgctcaactt cggtaacgct
     4141 cataccttta cggcgggcat aatcttcaac ctgatcgcgc tgaatttgtg ctacagcgta
     4201 gtacttgctg tccgggtggc tgaagtacca acccgaaacc gatgcaccgg gccacatggc
     4261 gaaagattct gtgagtttca tgccagtgtg tttttccact tccagcagct cccagatggt
     4321 ggctttttcc gtatgttccg ggcaggccgg atagcccggt gccggacgga tgccctggta
     4381 gttttcgcgg atcagttctt cgttgctgag gttctcgttc ggcgcatagc cccagtagac
     4441 tttacgcaca cgctcatgga gatactccgc aaaggcttcc gccagacggt cggcaagcgc
     4501 tttcaccatg attttgttgt aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc
     4561 ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa tctgctttac cagaaagctt
     4621 cggcgcaacg aagtcagcga gacagtagtt agcgaatccc gttttttcgg tctgttgacg
     4681 cagatggtgg ctgacgttga tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc
     4741 atcgcccaca cggtttgccg ggaacaggcc caccacgcca cgcggattca gcgttttctc
     4801 ggcgcttaat ttatccagca tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc
     4861 caccacttca tcttccagaa tgcgcggata cttcccggcc agcgaccagg tcataaagaa
     4921 cggtgtccag tcgatgtaat tacgcagcgt ttcgatgctg gcttcaactt cctgcacgcc
     4981 gagacggtgc gccaccggcg gcgtgtaagc ctgccagtca aaagcgaaat cgttatcgcg
     5041 cgccgcttcc agcgtgaccg gtggtgtgcg cggtttctta cgcccgtgct gaatacgtac
     5101 ggtttcgtac tccttgcggg tacgagcgac aaaatcatca cgctgagtat cggaaagcag
     5161 cgccgccacc acaccaacgg tgcgcgatgc gttctgtaca tacaccgtcg ggccgctgta
     5221 gttctgctca attttcaccg ccgtgtgcgc ttttgaggtc gtcgcgccgc caatcaatag
     5281 cgggatggtg aagccctgac gctccatctc tttcgccacg ttaaccatct cgtccagcga
     5341 cggcgtgata agccccgaaa gtccaatcag atcagcattc acttctttag cggtacggag
     5401 aattttttcc gcaggcacca taacgccgag atcgacaatt tcgtagttgt tacattgcag
     5461 caccacacca acgatatttt taccgatgtc gtggacgtcg cccttcacgg tggcgatcac
     5521 catcttgccg ttggttttgc cctgctcttt gctggcttca ataaacggtt cgagatacgc
     5581 caccgcctgt ttcatgacgc gcgccgattt gaccacctgt ggcaggaaca ttttcccttc
     5641 gccaaacaga tcgccgacca cgttcatgcc gtccatcaac gggccttcaa tcacttcaat
     5701 cgggcgcgta gcctgctggc gggcttcttc ggtatcctgc tcgataaact cggtaatgcc
     5761 tttaaccagc gagtattcca gacgtttatt cacttcccac gagcgccact ccgcctgctg
     5821 ggcgttagcg gtgtcgtcgg ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg
     5881 ctcagtgcca tcgtcgcgac ggttaagaat cacatcttcc accgcgtcgc gcagttcagc
     5941 gggcaggtcg tcgtaaatcg ccagttgccc ggcgttgacg atccccatat ccatgccatt
     6001 gcgaatagcg tagtagagga acaccgcgtg aatggcttcg cgcaccggat cgttgccacg
     6061 gaatgagaaa gaaacgttag atacaccgcc ggaaatcagc gcgtgcggca gttcgcgttt
     6121 gatatcttca cacgcgccga taaagtcctg cgcgtagttg ttgtgctctt caatgccggt
     6181 tgcgaccgcg aagatgttcg ggtcgaagat gatatcttct ggcgggaagc caacctcttc
     6241 ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc cgtgcgcggg tatcggcctg
     6301 tccctgttcg tcaaaggcca ttaccaccac tgccgcaccg taacgacgca agagcttcgc
     6361 gtgatggata aaggcgtcga cgccctcttt catcgagata gagttaacaa tgcctttgcc
     6421 ctggatacac ttcagacctt tttcaatgac atcccatttt gaggagtcga tcataatcgg
     6481 cacgcgagcg atatccggtt caccggcaat cagattgaga aaacgcacca tcgccgcttc
     6541 ggcgtcgagc atcccttcat ccatgttgat atcgataatc tgcgcaccgt tttccacctg
     6601 ctggcgggcc acatccagtg cctcgctgta tttctcttct ttgatcaggc gcttgaactt
     6661 agcggaaccg gtgacgttgg tgcgttcacc cacgttcaca aacaggctat cttcgccaat
     6721 gttcagcggc tccaggccgg acaaacggca ggctacgggg atttccggca gtttgcgcgg
     6781 cgctaatcct tctactgcac gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca
     6841 gccgccgacg atattgagaa aacccgcttg cgcccattca cgtatctgtt ttgccatcgt
     6901 gtcggcgtcg agatcgtact caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt
     6961 gacgtagcat tccgcaatcc gtgacagctc ctgcacgtac tggcgcagtt catcgggccc
     7021 cagcgcacag ttcaggccaa aggtcagagc ttcggcgtgg cgcaatgagt tgtaaaatgc
     7081 ttcggtggtc tgcccggaga gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat
     7141 aatcggcagc tcaacgccca gcgcttcaaa ctccgttttc accgcaaaaa ccgccgcttt
     7201 ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga tccgcgccac cttccaccag
     7261 cgctttggtg gattcgcgat aggccgccac cagctggtca aaagtgatgt tgcggaatgc
     7321 cggatcgttg acgtccggag aaatagacgc cgtgcggttg gttgggccga gaacaccggc
     7381 aacgtagcgc ggtttctccg gcgtgcgcgc ggtccactcg tcagcacagg cgcgcgccag
     7441 tttcgccgcc gcaaagttga tttccgccga cagggattcc atctggtaat ccgccatcgc
     7501 aatggtcgtg gagttgaagg tgttggtttc gatgatatcc gcgcccgctt caaagtaggc
     7561 gttgtggata gcggcgatca cttccggttt gctgagcacc agcaggtcgt tgttgccttt
     7621 gagatcgcat ggccagtcgg caaagcgttc accacgaaaa tcggcttcgt tcagtcgata
     7681 gctctggatc atggtgccca taccgccgtc cagcaccaga atacgttcat ttaactgcgc
     7741 acgcagttgt tccactttgc tgctcacact tgctcccgac acgctcaacc cagatttaat
     7801 aaaaattcaa caaaccatac tggcataaac gcatctgtgg taaaagcgac caccacgcaa
     7861 catgagattt gttcaacatt aactcatcgg atcagttcag caactattgc attagctaac
     7921 aataaaaatg aaaatgattt ccacgataca gaaaaaggag gctgtcatgg tcgcacccat
     7981 tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca ccagcgactg gacaggttca
     8041 gtctttaacg cgtggcctga aattactgga gtggattgcc gaatccaatg gcagtgtggc
     8101 actcacagag ctggcgcaac aagccgggtt acccaattcc acgacccacc gcctgctaac
     8161 cacgatgcaa cagcagggtt tcgtgcgtca ggtcggcgaa ctgggacatt gggcaatcgg
     8221 cgcacatgcc tttatggtcg gcagcagctt tctccagagc cgtaatttgt tagcgattgt
     8281 tcaccctatc ctgcgcaatt taatggaaga gtctggcgaa acggtcaata tggcggtgct
     8341 tgatcaaagc gatcacgaag cgattattat cgaccaggta cagtgtacgc atctgatgcg
     8401 aatgtccgcg cctatcggcg gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt
     8461 tttagcccaa ctgagcgaag aacaggtgac gaagctgctg caccgcaaag ggttacatgc
     8521 ctatacccac gcaacgctgg tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg
     8581 caaacggggt tattcatttg acgatgagga acatgcactg gggctacgtt gccttgcagc
     8641 gtgtattttc gatgagcacc gcgaaccgtt tgccgcaatt tcaatttccg gaccgatttc
     8701 acgtattacc gatgaccgcg tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga
     8761 agtgacgctg gcgtacggtg gaatgcgctg actttttctg gcgggcagag gcaatattct
     8821 gcccatcata cctgagtggc aatagaataa gggtgtctgt taatcgcatt gacgccaaaa
     8881 taacttaatg tcatacactt cacacacttt tattaactca gcattatttt taaacatcaa
     8941 actacttaat cattaacaaa atcg
//
LOCUS       DAPPNJ010000175         8929 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000175 DAPPNJ010000000
VERSION     DAPPNJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.176"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            72..5492
                     /locus_tag="RPM73_003570"
     CDS             72..5492
                     /locus_tag="RPM73_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDX6521890.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQNGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTADDMNVMPGG
                     ALRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5701..5967
                     /gene="hipB"
                     /locus_tag="RPM73_003571"
     CDS             5701..5967
                     /gene="hipB"
                     /locus_tag="RPM73_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDX6521891.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5967..7289
                     /gene="hipA"
                     /locus_tag="RPM73_003572"
     CDS             5967..7289
                     /gene="hipA"
                     /locus_tag="RPM73_003572"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDX6521892.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7601..7809
                     /locus_tag="RPM73_003573"
                     /pseudo
     CDS             7601..7809
                     /locus_tag="RPM73_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8243..8806
                     /gene="fimA"
                     /locus_tag="RPM73_003574"
     CDS             8243..8806
                     /gene="fimA"
                     /locus_tag="RPM73_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX6521893.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2433 a         1887 c         2403 g         2206 t
ORIGIN      
        1 ataagtttca tttttaatgt ctgacgactc atctctttcg tggctgtatt gtttgtatat
       61 ggagcgttta tatgaataga atctatcgcg tgatatggaa ttgcactcta caggtatttc
      121 aggcctgctc ggaattaact cgcagggcag gtaaaacatc gacggttaat ttgcgtaaat
      181 cctctggact gacaacgaaa ttcagtagat tgacgctggg tgttttgctg gcactaagcg
      241 gttcagcgtc tggtgcaagt ctggaagttg ataatgatca gattaccaat attgatactg
      301 atgttgctta tgatgcctac ctggttggct ggtatggcac tggagtgctt aatattttgg
      361 ctggcggtaa tgcctcctta accactatta ctaccagcgt cattggcgct aatgaggact
      421 cagagggcac cgttaatgtt ttgggtggca cctggcgatt gtatgatagc ggaaataatg
      481 caaggccttt aaatgtgggt caatccggaa cggggacgct gaatattaaa cagaatggtc
      541 acgttgatgg aggctattta agactaggtt cctcgacagg aggcgtcggg acggtcaatg
      601 ttgagggaga ggactctgtt ctgacgaccg aattattcga aatagggagt tacggtacgg
      661 gctcattaaa tattacggat aagggttacg tcacgagttc aatagtcgcc attttaggct
      721 atcaggcggg cagtaatggc caggttgtcg ttgaaaaggg cggtgagtgg ctaataaaaa
      781 ataacgattc ctcaattgaa tttcaaattg gtaatcaagg aactggggaa gcgactattc
      841 gcgagggtgg actgattacg gctgaaaaca cgattatcgg tggcaatgct accggtatcg
      901 gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat
      961 atttcggtaa tggcacactc aatatttcca ataatggact aattaataac aaagaatatt
     1021 cattagtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt
     1081 ggaatttcct cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg
     1141 gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga
     1201 aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg
     1261 gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgaa gggcttgttg
     1321 tcagcaacgg aggaagctca ctcggttatg gagaaaccgg cgtcgggaat gtcagcatca
     1381 ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg
     1441 tcggaaacct caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg
     1501 gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata
     1561 ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca
     1621 cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta
     1681 atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac
     1741 aggtcggtgt attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc
     1801 gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga
     1861 actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc
     1921 tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac
     1981 ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt
     2041 atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc
     2101 atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag
     2161 acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg
     2221 gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa
     2281 cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat
     2341 caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc
     2401 gagtgcagct gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat
     2461 tcgccggtgt tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg
     2521 cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag
     2581 agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc
     2641 ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg
     2701 actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg cttatcgacg
     2761 tgcctaaacc gtggaatgat cccatggcga ataaccctct gacgacgctc aatttgctgg
     2821 aacacgacga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg
     2881 gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac aaaacgttac
     2941 acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc ctcacgaccg
     3001 caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc catggtgggc
     3061 aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat atgtcggcaa
     3121 aaatcggtgg tgaaggggat ctggcaatca atacggtgcg acaggtttcg ctttccaacg
     3181 gtcagaacga ctatcaaggg gcaacctacg ttcagatggg gacattacgt accgatgcgg
     3241 atggtgcact tggcaacacc cgggaactga acatcagcaa cgcggccatc gtcgatctta
     3301 atggatcgac gcagacggta gagacattca ccgggcagat gggttcgact gttttgttca
     3361 aagagggggc gctgacggta aataaaggtg ggatcagtca gggtgaactg acaggtggcg
     3421 gaaacctgaa tgttacaggg ggaacgctgg ctatcgaggg gcttaatgca cgctacaatg
     3481 cgttaaccag cattagccca aatgcggaag tcagcctcga taatactcag gggttaggca
     3541 gaggaaatat tgccaatgac ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgca
     3601 atagcataag cgggaagggt atcgtgagcg caaccgccag gacagatgta gagctggatg
     3661 gcgataatag ccgctttgtg gggcaattca acattgatac aggcagcgcg ctcagcgtca
     3721 acgagcagaa aaacctgggt gatgcttccg ttatcaataa tggcctgctc accatctcca
     3781 ctgagcgtag ctgggcgatg acgcacagta tcagcggtag cggtgatgtg acaaaactgg
     3841 gtaccgggat cctgactctt aacaacgatt ccgcggcgta tcagggtacg acggatatcg
     3901 tgggggggga aattgctttc ggttccgact ctgccattaa tatggcaagt caacacatta
     3961 atatccataa cagcggtgtg atgtcgggaa atgtcaccac tgcagatgat atgaacgtta
     4021 tgcctggggg ggcactgcgt gtcgctaaaa ccactatcgg cggcaacctg gagaatggcg
     4081 gcacggttca aatgaatagc gaagggggga aaccggggaa tgtactgacc gttaacggca
     4141 actataccgg aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc gataattcgc
     4201 ccaccgataa gatgaacgtg aaaggcgata cccaagggaa cactcgcgtt cgggttgata
     4261 acattggcgg cgtcggtgca caaacggtca acggtattga actcattgag gttggcggta
     4321 attctgcagg taacttcgcg ctgaccaccg gaactgtcga agctggggct tacgtctaca
     4381 cgctggctaa agggaagggg aatgacgaga aaaactggta tctgaccagt aaatgggacg
     4441 gcgtaacgcc agcggataca cccgatccca tcaataatcc ccctgttgtg gatccggaag
     4501 gcccatcagt ttatcgcccg gaggccggaa gctatatcag caacattgcc gcagccaact
     4561 cgctgtttag ccatcgttta cacgaccgtc tgggtgagcc gcagtataca gattcactgc
     4621 attctcaggg gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa
     4681 gggccggtga cggtcagcta aatactcagg ctaaccgcta tgtattgcag ctaggcggcg
     4741 atttggcgca gtggagtagc aacgcgcagg atcgctggca tcttggcgtg atggcaggct
     4801 acgccaatca gcacagtaat actcagagta atcgtgtggg ttataaatcg gatgggcgca
     4861 tcagcggtta cagcgctggg ctgtacgcga cctggtatca gaacgatgcg aataagaccg
     4921 gcgcttatgt tgacagctgg gcgctgtata actggtttga taacagcgtc agttccgata
     4981 accgttctgc tgacgactat gattctcgcg gtgtgacggc ctctgttgag ggtgggtata
     5041 cctttgaagc gggaacattt agcggcagcg aagggacgct gaatacctgg tacgtccagc
     5101 cacaggcgca aatcacctgg atgggtgtga aagattccga ccatacccgg aaagacggaa
     5161 cgcgcattga aacggaaggc gacggaaatg tgcaaacgcg acttggggtg aaaacctacc
     5221 tgaatagcca tcaccagcgt gacgatggta aacagcgtga gttccagcct tacattgaag
     5281 cgaactggat caacaatagc aaagtctacg ccgtgaagat gaatggtcaa accgtaggcc
     5341 gtgaaggtgc gcgtaatctc ggtgaagtac gtaccggggt tgaggcgaaa gtaaataaca
     5401 accttagcct gtgggggaat gtcggtgtgc aactaggtga taaaggctat agcgatactc
     5461 agggcatgct gggagtgaaa tatagctggt aaaccgtata agccgcatgt cgagatggca
     5521 tgcggcttaa tattgccgac ttcaaaggct ttaaagcctc ccttttatcc gcgatcgcgg
     5581 atatcgcagc gtttatcccg tacagcggat aagatgtgtt tccagattga ctttatcctc
     5641 actaaaggat aaaactgata atatcccctt aagcggataa atttgctgtg gacgtatgac
     5701 atgatgagct ttcagaagat ctatagccca acgcaattag cgaatgcaat gaaactggtt
     5761 cgccagcaaa atggctggac gcagagcgag ctggcgaaaa aaattggcat taagcaggcg
     5821 acaatttcca atttcgaaaa caaccctgac aataccacgc tcacgacatt ttttaagatt
     5881 ttacagtcgc ttgaactctc aatgacacta tgcgacacga aaaatgcctc gccagaatca
     5941 acagaacagc aagatctgga gtggtaatgc ctaaacttgt cacttggatg aacaaccagc
     6001 gggtaggcga gttaacgaag ttagccaacg gcgcgcacac ctttaagtat gcaccggagt
     6061 ggttagccag ccgttatgcc agaccgttgt cactttcgct gccattgcag agggggaata
     6121 tcacctcaga tgccgtattt aacttcttcg ataacctgtt acccgatagc ccgattgtac
     6181 gtgaccggat cgttaaacgt tatcatgcta aatccagaca accgtttgat ttattgtcag
     6241 aaatagggcg agacagcgtt ggtgccgtaa cgttattacc cgaaaatgaa accataacgc
     6301 gcccgataat ggcatgggaa aagcttactg aagccaggct tgaagaagta ttaacggctt
     6361 ataaagcaga tatccctctg ggcatgatta gagaagaaaa tgactttcgc atctcggttg
     6421 ctggcgcgca ggagaagaca gcgctgctta gaataggcaa tgactggtgc attccgaaag
     6481 gaataacgcc gacgacgcac atcattaaat taccgattgg cgaaatcagg cagcccaatg
     6541 cgacgctcga tctcagccaa agcgttgata atgagtatta ctgtctgctg ctggcgaaag
     6601 aacttgggtt gaatgttccg gacgcagaaa tcattaaagc gggaagggtg cgcgcgttag
     6661 cggtcgaacg ttttgacagg cgttggaata ctgagcgaac ggttttactt cgcttgccac
     6721 aggaggatat gtgtcagaca ttcggtttac cttcatctgt gaaatatgaa tcagatggag
     6781 gcccaggcat cgcgcggatc atggcgtttt tgatggggtc cagcgaggcg cttcgcgatc
     6841 gttatgattt tatgaaattc caggtcttcc agtggttgat tggcgcaacg gatggccatg
     6901 caaaaaactt ctccgtattt attcaggctg gcggcagtta tcgactcacg ccattttacg
     6961 acatcatttc agcatttccg gtccttggcg gtacgggaat acacatcagc gatctcaaac
     7021 tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc
     7081 gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc
     7141 taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga
     7201 cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac
     7261 ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcac
     7321 gacaatcatg accgccaacg actggattct tccttggttt atactggtgc gcataatgta
     7381 tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg ttattcgttc
     7441 agggttcaag aacttaagcc tctcctcttt cataattggc ctgtgcatgg agaaatagcc
     7501 tggttattcc ctgccccatg ctaagagcgc agtgtctgtc gcatagacac tgcatacgaa
     7561 aacaccagag ggtgctgtta cttatttaag gacggatcaa gcgggaatga gtccactccg
     7621 gtatcagaaa tggctacgtc tcaatgaagt caggcgaccg atgctgaatg aacattacga
     7681 tgtcaccaca gcagcctatg ctgtcggtta cgaaagctat cccatttcag tcgggaatat
     7741 tcgcggatgt ttggagagtc acccaagaga gatattacca ggttaagaaa gtctgtcggt
     7801 cagctttaat aaaccctgca cttatctgtt tagagcaatt cggtgttagt ttcagcaagc
     7861 aaacattaac catagctaat gatttatagc catattaacc attagtagcc cttgaccttt
     7921 cctgacactt cccttgttcg caataatatt ctgtttagtc acggtttata aataacatgc
     7981 ataatgaaat cgtcatgtta tttagcgaca taaaatgttt ttgcaaatac tctcttcaac
     8041 aagtgcagca ataaacttgt tgtgattagg tgtccttgtg atttaaagat attatcgttc
     8101 ttcgtgaaat gtaataaccc tggaaactat attctcaggg caacaaccaa tcaagaatga
     8161 ttcttgtttt ataagcaata acagaatgac ttaattgtta actctcttta ttcagctact
     8221 taaatataaa ttttggagaa ttatgaaact caaacatgtt ggtatgattg tcgtttctgt
     8281 gttggcgatg tcgtctgctg cggtaagcgc agccgagggt gatgaatcag taacgaccac
     8341 tgttaatggc ggtgttattc attttaaagg tgaagtggta aatgccgctt gtgcaattga
     8401 ttcagaatca atgaaccaaa cggttgagct gggtcaggtt cgttcttctc gcctggctaa
     8461 agcgggtgac ctcagctccg ccgttggctt caatatcaag ctgaatgatt gtgataccaa
     8521 tgtttccagt aatgcagctg ttgcattcct gggtactact gtcaccagta atgacgatac
     8581 gttagcgctg cagagttcag cggcaggctc tgcccaaaat gtcggtattc aaattttgga
     8641 ccgtacgggt gaggtattaa tacttgatgg ggccactttt agtgctaaaa ccgacttgat
     8701 tgatggcacg aatatactgc cattccaggc tcgttatatt gctctcgggc agtccgtagc
     8761 tggtactgca aacgctgatg cgaccttcaa agttcaatat ctataattca atcttaaggt
     8821 ggctatacgt catctgaaat tgaaacaggg tgtgtgtggc atggctacgc acacccgagc
     8881 aatttaccaa tattcatctc atttttaatg agaaatggtt tgtatttgg
//
LOCUS       DAPPNJ010000176         8726 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000176 DAPPNJ010000000
VERSION     DAPPNJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.178"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RPM73_003575"
     CDS             complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RPM73_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDX6521894.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1536..1766
                     /gene="chaB"
                     /locus_tag="RPM73_003576"
     CDS             1536..1766
                     /gene="chaB"
                     /locus_tag="RPM73_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDX6521895.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1924..2619
                     /gene="chaC"
                     /locus_tag="RPM73_003577"
     CDS             1924..2619
                     /gene="chaC"
                     /locus_tag="RPM73_003577"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDX6521896.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RPM73_003578"
     CDS             complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RPM73_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDX6521897.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPVEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3201..4595
                     /gene="ychO"
                     /locus_tag="RPM73_003579"
     CDS             3201..4595
                     /gene="ychO"
                     /locus_tag="RPM73_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDX6521898.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RPM73_003580"
     CDS             complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RPM73_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDX6521899.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RPM73_003581"
     CDS             complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RPM73_003581"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDX6521900.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7374..>8726
                     /gene="narK"
                     /locus_tag="RPM73_003582"
     CDS             7374..>8726
                     /gene="narK"
                     /locus_tag="RPM73_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDX6521901.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         2129 a         2123 c         2267 g         2207 t
ORIGIN      
        1 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
       61 gctcgcaatt ctgattaacg agctgtccag gtagtatgaa gaaatacaat aagcaggaat
      121 ttatcttatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
      181 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
      241 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
      301 accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
      361 ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg
      421 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagagccac
      481 caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct
      541 ggcattcatt ttggtgactg caataacggc aatcagatgg ataatcaacc agatagcatg
      601 ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat cactgtcatc
      661 ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc
      721 atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc
      781 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
      841 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
      901 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
      961 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
     1021 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
     1081 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
     1141 gatggcaatg accactggta gtgtctggct acttccccac aggaacagca ctaccagcgc
     1201 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
     1261 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggga
     1321 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cgctgtcgat
     1381 tttttactca ttggggcata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
     1441 tcttaagaag agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
     1501 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
     1561 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
     1621 tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac gccagtcgcg
     1681 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
     1741 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
     1801 ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag
     1861 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
     1921 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
     1981 gaagaatcac tcttatggtc agcagaacaa cgggcggctt cgctggcggc gacgctggct
     2041 tgtcgacctg atgaggaacc ggtgtggatc ttcggctatg gatcgttgat gtggaatccg
     2101 gcactggagt ttaccgaatc gtgcaccggt acactggttg gatggcatcg cgcattttgc
     2161 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
     2221 gagggcggac gcaccacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa
     2281 ctaaccttgt tgtggaagcg agagatgatt accggctgtt atctgccaac ctggtgtcag
     2341 cttgatcttg atgatggacg cacagtaaac gccattgtgt ttattatgga tccgcgacat
     2401 ccagaatatg aatctgatac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
     2461 ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg
     2521 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag
     2581 aattttccgg atggtgtgtt acgtccggga ttcgcctgag taaacttccc gcatagtggg
     2641 gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
     2701 gcgccagttc cacaagagta ccgatttcca ccccatcaat cagaggaagt gtactaatcc
     2761 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
     2821 ccagcatttg ctgaatgttg tagccttcca ctggtttttg cccgcgcaac ccggctgtga
     2881 ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
     2941 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
     3001 tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacactca tcttctatct
     3061 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
     3121 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
     3181 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
     3241 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
     3301 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
     3361 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
     3421 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
     3481 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg
     3541 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
     3601 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
     3661 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
     3721 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
     3781 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg tttgctgcat
     3841 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac ctgacagccc
     3901 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
     3961 gtgatcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
     4021 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
     4081 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
     4141 tcaaaaaaca actttctgcg ggtgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
     4201 atgacaatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
     4261 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
     4321 tacaaatccg cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat
     4381 taagtttgac gccgggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
     4441 actggcagaa cggggaaagg gcgagcaatc actggcgatt gtctgtggtg gtggaagata
     4501 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg
     4561 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
     4621 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
     4681 acttttactg tgctttcggt gatgtccagg cggcgggcaa tcatcttgtt cggcaaaccc
     4741 tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgctca
     4801 gtagtggcac ggttagcacg caagctggcg gccagaacag gcgttaatgc ttcgcttaat
     4861 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
     4921 tttaacagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
     4981 gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
     5041 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagac
     5101 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt
     5161 gccatactga taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg
     5221 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
     5281 gggaataaag gtgaccacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc
     5341 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
     5401 ggcattttca ggcacgccgc agccgttatc ctgtacggtc agtttgacct gattatcgtt
     5461 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
     5521 ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa
     5581 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc
     5641 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
     5701 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
     5761 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tacaagagag
     5821 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
     5881 caactgttgc tgacgttcct gatggcgatc caacgccagc gtggcggtga gttgttcaac
     5941 cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc
     6001 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg ttgtgccgcg
     6061 atcgccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
     6121 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtatcat aaacccgcag
     6181 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtacaggtg acaggcgttc
     6241 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaatg agaggatctg
     6301 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
     6361 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
     6421 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
     6481 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
     6541 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcga tgcgcatttc
     6601 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
     6661 cgctgacacc gtttctcggt tttgtgcacg catcagcgca gggatcagtt cattacgcca
     6721 gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
     6781 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
     6841 tggcactgcc gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
     6901 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
     6961 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
     7021 acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga
     7081 atgtgacgat gcattctgga atggcagtat tctcggctat tggctgaagt atacccatac
     7141 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
     7201 gattttacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
     7261 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
     7321 tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
     7381 actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc
     7441 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg taacctgtgg atttccgttc
     7501 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
     7561 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcag
     7621 tttctggcgc gttattacgt gttccatact cctttatggt tccaatcttc ggtggtcgtc
     7681 gctggacggc gttcagcacc ggtattctga ttattccttg tgtctggctg ggttttgccg
     7741 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgctg tgcggctttg
     7801 ctggcgcgaa cttcgcttcc agtatggcaa acatcagctt cttctttccg aaacagaagc
     7861 agggtggcgc gctgggcctg aatggtggtc tgggcaacat gggcgttagc gttatgcagt
     7921 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
     7981 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
     8041 ttgccatctt caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
     8101 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
     8161 atctggcaac cttcggctcc tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
     8221 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
     8281 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
     8341 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg
     8401 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
     8461 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
     8521 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca
     8581 cggcggcggc gttgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga
     8641 aagcgtttgg tagctcgctg gcattaacgg gttcgccagt cggcgcaatg aaggtatttt
     8701 tgattttcta tatcgcctgc gtagtg
//
LOCUS       DAPPNJ010000177         8642 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000177 DAPPNJ010000000
VERSION     DAPPNJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.179"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(295..558)
                     /gene="rpsT"
                     /locus_tag="RPM73_003583"
     CDS             complement(295..558)
                     /gene="rpsT"
                     /locus_tag="RPM73_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDX6521902.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            661..879
                     /locus_tag="RPM73_003584"
     CDS             661..879
                     /locus_tag="RPM73_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDX6521903.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            887..1828
                     /gene="ribF"
                     /locus_tag="RPM73_003585"
     CDS             887..1828
                     /gene="ribF"
                     /locus_tag="RPM73_003585"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDX6521904.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1871..4687
                     /gene="ileS"
                     /locus_tag="RPM73_003586"
     CDS             1871..4687
                     /gene="ileS"
                     /locus_tag="RPM73_003586"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDX6521905.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4687..5181
                     /gene="lspA"
                     /locus_tag="RPM73_003587"
     CDS             4687..5181
                     /gene="lspA"
                     /locus_tag="RPM73_003587"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDX6521906.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5269..5718
                     /gene="fkpB"
                     /locus_tag="RPM73_003588"
     CDS             5269..5718
                     /gene="fkpB"
                     /locus_tag="RPM73_003588"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX6521907.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5720..6670
                     /gene="ispH"
                     /locus_tag="RPM73_003589"
                     /gene_synonym="lytB"
     CDS             5720..6670
                     /gene="ispH"
                     /locus_tag="RPM73_003589"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDX6521908.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6736..7650
                     /gene="rihC"
                     /locus_tag="RPM73_003590"
     CDS             6736..7650
                     /gene="rihC"
                     /locus_tag="RPM73_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDX6521909.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7817..8638
                     /gene="dapB"
                     /locus_tag="RPM73_003591"
     CDS             7817..8638
                     /gene="dapB"
                     /locus_tag="RPM73_003591"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDX6521910.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLNGKKSGLFDMRDVLDLNNL"
BASE COUNT         1968 a         2152 c         2462 g         2060 t
ORIGIN      
        1 attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat atccacattc
       61 tttgtagggg agtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt
      121 tctacgattc ttaagccacg aagagttcag aaagtgtaac ggcatgtctg caaaagccag
      181 catcttttga ctatctggca accggtagtg tgttctctca cgcatcataa aagcagcagg
      241 cataaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc
      301 cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat
      361 cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc
      421 agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat
      481 agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc
      541 tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc
      601 cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata agcgccgtta
      661 atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc ggggtgtgaa
      721 tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt gatttgcgcc
      781 atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc
      841 gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg
      901 cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta ttggtaattt
      961 cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa
     1021 gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac
     1081 cgataaagcc ccggcacggc tgacccggct gcgggaaaaa ctgcgttacc ttgcagagtg
     1141 tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt taaccgcgca
     1201 aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg ccgtaggtga
     1261 tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga aagctggcat
     1321 ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggcg tgcgtatcag
     1381 cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag aaagtttact
     1441 ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat
     1501 aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta
     1561 tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acattggaac
     1621 acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag atgttgcaat
     1681 ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg
     1741 atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga
     1801 attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac
     1861 cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat
     1921 gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct
     1981 gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc
     2041 tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat
     2101 tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg
     2161 ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg gtgagaaatt
     2221 caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca
     2281 acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat
     2341 ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg gcaacggtca
     2401 cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg cgctggcgga
     2461 agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt ttcaggcggt
     2521 cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc caatctcgct
     2581 ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc
     2641 tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct
     2701 ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa
     2761 aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg acgttccggc
     2821 aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca ccgcgcctgg
     2881 ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt
     2941 tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt
     3001 caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga
     3061 gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc
     3121 gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga
     3181 gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggttgc
     3241 taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga tgtcactgtt
     3301 cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg aagaagtggc
     3361 aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga aagagatcct
     3421 cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc
     3481 cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga
     3541 catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc tgatgatctc
     3601 caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct ttaccgtgga
     3661 tggtcagggt cgcaagatgt ctaaatccat cggcaacacc gtttcgccgc aggatgtgat
     3721 gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaactgact acaccggtga
     3781 aatggccgtt tctgatgaga tcctgaaacg tgctgccgac agctatcgtc gtatccgtaa
     3841 caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag atatggtgaa
     3901 accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga
     3961 agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg
     4021 cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac
     4081 cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc acatcgcaga
     4141 agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta
     4201 cctgccgggc gaacgtgaaa aatacgtctt taccggtgag tggtacgaag gcctgtttgg
     4261 tctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg
     4321 cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg gctcgctgga
     4381 agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg cgctgggcga
     4441 tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata acgacgcacc
     4501 tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga gtaaagccga
     4561 aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca
     4621 cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt
     4681 tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc
     4741 gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat
     4801 acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt
     4861 agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat tgcgattggt
     4921 attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat
     4981 atcgcttatg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac
     5041 ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac
     5101 cttgccgata ctgccatttg tgtcggtgcg gcactgattg tgctggaagg ttttttgcct
     5161 tctaaagcga aaaaacaata ataaaccctg ccggataagg cctacagatt gctgcgaaat
     5221 cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat gtctgaatct
     5281 gtacagagca atagcgccgt cctggtgcac ttcacgctaa aactcgacga tggcaccacc
     5341 gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggtga tgcttctctt
     5401 tctgaagggc tggagcaaca cctgttgggg ctgaaagtgg gcgataaaac caccttctcg
     5461 ttggagccag atgcggcgtt tggcgtgccg tcaccggacc tgattcagta cttctcccgc
     5521 cgtgaattta tggatgcagg cgagccagaa attggcgcaa tcatgctttt taccgcaatg
     5581 gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat taccgttgat
     5641 ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct ggaaatcgat
     5701 ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt tttgtgccgg
     5761 ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc atttacggcg caccgatata
     5821 tgtccgtcac gaagtggtac ataaccgcta tgtggtcgat agcttgcgtg agcgtggggc
     5881 tatctttatt gagcagatta gcgaagtacc ggacggcgcg atcctgattt tctccgcaca
     5941 cggtgtttct caggcggtac gtaacgaagc aaaaagtcgc gatttgacgg tatttgacgc
     6001 gacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgctagtc gccgtggcga
     6061 agaatctatt ctcatcggtc acgccgggca cccggaagtg gaaggcacga tgggtcagta
     6121 cagcaacccg gaagggggaa tgtatctggt tgaatcacca gacgatgtgt ggaaactgac
     6181 ggtcaaaaac gaagagaaac tctcctttat gacccagacc acgctgtcgg tggatgacac
     6241 gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc cgcgcaaaga
     6301 tgacatctgc tacgccacga ctaaccgaca ggaagcggta cgtgccctgg ccgaacaggc
     6361 ggaagttgtg ctggtggtcg gttcgaaaaa ctcctctaac tccaaccgtc tggcggagct
     6421 ggcccagcgt atgggcaaaa gcgcgttttt gattgatgat gcgaaagata tccaggaaga
     6481 gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc cggatattct
     6541 ggtgcagaat gtggtggcac gtttgcagca gctgggcggt ggtgaagcca ttccgctgga
     6601 aggccgtgaa gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg atattcgtga
     6661 agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt taccggcatt
     6721 ttttatggag aaaacatgcg tttacctatc ttcctcgata ctgaccccgg cattgacgat
     6781 gccgtcgcca ttgccgccgc gatttttgca cccgaactcg acctgcaact gatgaccacc
     6841 gtcgcgggta atgtctcggt tgagaaaact acccgcaatg ccctgcaact gctgcatttc
     6901 tggaatgcgg agattccgct cgcccaaggg gccgctgtgc cgctggtacg cgcaccgcgt
     6961 gatgcggcat ctgtgcacgg cgaatcggga atggctggct acgactttgt tgagcacaac
     7021 cgaaagccgc tcgggatacc ggcgtttctg gcgattcggg atgccctgat gcgtgcacca
     7081 gagcctgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt actttcacaa
     7141 tgcccggaat gcaagccgta tattcgccgt ctggtgatca tgggtggttc tgccggacgc
     7201 ggcaactgta cgccaaacgc cgagtttaat atcgctgccg atccagaagc ggctgcctgt
     7261 gtcttccgta gcggaatcga aatcgtcatg tgcggtctgg atgtcaccaa tcaggcaata
     7321 ttaacccctg actatctcgc tactttgccg gagttgaatc gcaccggaaa aatgcttcat
     7381 gcactgttta gccactaccg tagcggcagt atgcaaagcg gtctgcgaat gcacgatctc
     7441 tgcgccatcg cctggctggt gcgcccggac ctgttcactc tcaaaccctg ttttgtggca
     7501 gtggaaactc agggcgaatt tacctcaggc acgacggtgg ttgatatcga cggttgcctg
     7561 ggcaagccag ccaatgtaca ggtggcattg gatctggatg tgaaaggctt ccagcagtgg
     7621 gtggctgagg tgctggctct ggcgtcgtaa cctgtcacat gttattggca tgcagtcatt
     7681 catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc taattatcag
     7741 cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct atgcaaatta
     7801 acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg gagccggggg
     7861 gcgtatgggc cgccagttga ttcaggcggc gctggcatta gaaggcgtgc agctgggcgc
     7921 ggcgctggag cgcgaaggat cttctttact gggcagcgac gccggtgagc tggctggtgc
     7981 agggaaaaca ggcgttaccg tgcaaagcag cctcgatgcg gtaaaagatg attttgatgt
     8041 gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt gtcgccagca
     8101 tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggtaaac aggcgattcg
     8161 tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg gcgttaacgt
     8221 catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg atatcgaaat
     8281 tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac tggcaatggg
     8341 agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct acagtcgtga
     8401 aggccatacc ggtgaacgtg tgcctggtac cattggtttt gccaccgtgc gtgcaggtga
     8461 catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg agatcaccca
     8521 taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt tgtggttgaa
     8581 tggtaagaaa agtggtcttt ttgatatgcg agatgtactt gatctcaata atttgtaacc
     8641 ac
//
LOCUS       DAPPNJ010000178         8633 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000178 DAPPNJ010000000
VERSION     DAPPNJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.180"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            186..752
                     /gene="mepS"
                     /locus_tag="RPM73_003592"
     CDS             186..752
                     /gene="mepS"
                     /locus_tag="RPM73_003592"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDX6521911.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            933..2489
                     /locus_tag="RPM73_003593"
     CDS             933..2489
                     /locus_tag="RPM73_003593"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDX6521912.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2571..4385
                     /locus_tag="RPM73_003594"
     CDS             2571..4385
                     /locus_tag="RPM73_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX6521913.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPVFPEKYWKDRKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            4386..5480
                     /locus_tag="RPM73_003595"
     CDS             4386..5480
                     /locus_tag="RPM73_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDX6521914.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5480..6505
                     /locus_tag="RPM73_003596"
     CDS             5480..6505
                     /locus_tag="RPM73_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX6521915.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6507..8096
                     /gene="yejF"
                     /locus_tag="RPM73_003597"
     CDS             6507..8096
                     /gene="yejF"
                     /locus_tag="RPM73_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDX6521916.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILRLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8100..8444)
                     /locus_tag="RPM73_003598"
     CDS             complement(8100..8444)
                     /locus_tag="RPM73_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521917.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2031 a         2166 c         2171 g         2265 t
ORIGIN      
        1 cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca tataattttg
       61 tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg taattagtct
      121 cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa
      181 acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatc cccgcgattg
      241 cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg
      301 agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa
      361 acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag
      421 gcgtacgtta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac
      481 agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttac gaacagcagg
      541 aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt ctgttccgtg
      601 ccggttcaac gggacgccat gtcggtattt atatcggcaa caatcagttt gtccatgctt
      661 ccaccagcag tggtgttatt atttccagca tgaatgaacc gtactggaag aagcgttaca
      721 acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct
      781 atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt cattcatcag
      841 agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa
      901 tgttgtcgta atttcaggaa attaacggaa tcatgttcat acgcgctccc aattctggac
      961 gtaagctcct gcttacctgc attgtagcag gcgtgatgat tgcgatactg gttagctgcc
     1021 ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg attatcgacg
     1081 tacaaaagta tctcgatacc tattttgccg acctgaaatc cactactgac cggcttcagc
     1141 cgctgacctt agatacctgc cagcaggcta accccgaact gaccgctcgc gcggcgttta
     1201 gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa aacattctgt tcatctgcga
     1261 ctggtgagat ggacattcca ctcaatgaat tgattccggc gctcgacatt aataaaaacg
     1321 tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct
     1381 ggtatcgcaa ccctttgctg aaaaatagcg gcgtctttgc cgctctgaat ctcaacctga
     1441 cgccttcact cttttatagt tcacggcagg aagattacga tggcgtcgcc ctcattattg
     1501 gcaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa ttaaccgaca
     1561 tgccggtccg tgaaactaaa attgcgggca ttcctctgac cgttcggctt tatgcagatg
     1621 actggacatg gaacgatgtg tggtacgcat ttttactggg cggcatgagt ggaactgtcg
     1681 ttggcctgct ctgctattac ctgatgagcg tacgtatgcg ccccggcaga gaaatcatga
     1741 ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc ggtggtggat acacaagctt
     1801 tacgagtcac gggcctggaa gtactgctac gctggcggca tcctgtagcg ggagaaattc
     1861 ccccggatgc cttcattaac tttgccgaat cgcaaaagat gattgtgccg ctgactcagc
     1921 acctgtttga gttaattgcc cgcgatgccg cagaattaga aaaagtgctg ccggtaggcg
     1981 tcaaatttgg cattaacatt gcgccggacc atctgcacag cgaaagcttt aaagcagata
     2041 tccagaaact gctcacttcc ctacccgcac accatttcca gattgtgctg gaaattaccg
     2101 agcgcgatat gctgaaagag caagaagcca cacaactctt cgcctggctg cactcggtcg
     2161 gcgtagaaat tgctattgat gacttcggca ccgggcacag cgcgcttatc tatcttgagc
     2221 gttttacgct cgattatctg aaaattgacc gtggatttat caacgccatc ggtacggaaa
     2281 cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgcctc aatatgctga
     2341 cggttgctga aggggtcgaa acgccggaac aggcgcgatg gctaagcgaa cgcggcgtta
     2401 atttcatgca aggctactgg attagtcgcc cgttaccgct ggacgatttt gtccgctggc
     2461 tgaagaaacc gtatacgccg cagtggtaag gtatgcttac gtcccttatt attcatagtg
     2521 aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc
     2581 gcatactgct gctgtttatc gctctgttca ccttcggtgc gcaggcgcag gctatcaagg
     2641 aaagctatgc ctttgccgtg ctgggcgaac cccggtacgc gtttaatttc aaccattttg
     2701 attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac gttgtcagcc ctcggcacct
     2761 tcgataattt caaccgctat gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt
     2821 acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg
     2881 aaagcgcacg ctatgctgac gattattcct gggtggaggt cgctattaat ccgcgcgccc
     2941 gttttcatga tggttcgccc attactgccc gcgatgtaga gtttactttt caaaaattta
     3001 tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg
     3061 caccgttaac cgtgcgcatt gagttagcta aacccagcaa agaagatatg ttgagtctgt
     3121 tttcgctgcc ggtatttcca gaaaagtact ggaaggatcg caaacttagc gatccgctcg
     3181 ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc ctggaaaatg gggcaaaata
     3241 ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgtggacgct
     3301 ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt
     3361 ttaaagcagg tgcctttgat ttgcgtatgg aaaacgacgc taaaaactgg gccacgcgtt
     3421 ataccggtaa aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag
     3481 cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc gatcgccggg
     3541 tccgggaagc aatcactctc gcctttgact ttgaatggat gaacaaggcg ttgttttaca
     3601 atgcctggag tcgcacgaac agttattttc agaataccga atacgcggcc agaaattacc
     3661 ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg tcagaagtct
     3721 tcacacaaat ctaccagccg ccggtatcta aaggcgatgg ctacgatcgt gacaacctgt
     3781 taaaagccga caaacttctc aacgaagcgg gctgggtgct gaagggtcag caacgcgtta
     3841 atgccacaac gggtcagcca ctcagctttg aattattgct tcccgcaagc agcaatagtc
     3901 agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg gacattcgca
     3961 aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac atgatgccgc
     4021 gcctatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat
     4081 atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac tcgctgatca
     4141 accaaattat tgccgcgcag ggaaataaag aaaaattatt gccgttgggg cgagcactgg
     4201 atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc
     4261 tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg
     4321 atacctggtg gtatgacgtt aataaagcgg ccaaactgcc gtcagccaga caacagggag
     4381 agtagatggg cgcttacctg attcgccgtc tgttgctggt gatcccaaca ttatgggcga
     4441 ttatcaccat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca
     4501 tcgccgccat tgagtttggt aatgccggag tattacccgg cgcaggcggt gaaggtgttc
     4561 gtgccagcca tgcgcaaacg ggtgtcggca atatcagcga cagtaattac cgtggcggac
     4621 gcggattaga tccagaagtg atcgccgaga tcactcatcg ctacggtttc gataagccga
     4681 tccacgaacg ttacttcaaa atgctctggg actacatccg ctttgatttt ggcgatagcc
     4741 tgtttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtt tccatcaccc
     4801 tcggattgtg gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag
     4861 ctgttcataa tgggagccgc tttgacgtct ggagtagcgc atttatcatc atcggctacg
     4921 ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcaggc ggcagctatt
     4981 tcgacctgtt ccctctacgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc
     5041 agaaaatcac cgattatctg tggcatatca cgctaccggt gctggcgaca gtgattggtg
     5101 gctttgcggc gctgaccatg ctgaccaaaa actcattcct tgatgaagtg cgcaagcaat
     5161 acgtggtgac cgcgcgtgcg aaaggggtaa gtgagaaaaa cattctctgg aaacatgtgt
     5221 tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt
     5281 ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggccttggc ttactgggct
     5341 acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc
     5401 tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtt gatccgcgta
     5461 ttgattttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc gttgggcgcg
     5521 ttttcgtcat aaccgccgcg gctactggtc gttatggatt ttcctcgtct tgtttggttt
     5581 gagtttgtgt tctgagctta tcgccaacga taaaccgttg ctggtacgtt atgacggcag
     5641 ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc cgctggcaag
     5701 tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataacg gctgggtact
     5761 gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc
     5821 ttctcccccc tcccggcaaa actggctggg aacggatgcc aacggcggcg atgtgctggc
     5881 acgcattctc tatggcacgc ggatctcggt tctgtttggc ctgatgctga ctctctgttc
     5941 cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggcggta aagtcgatct
     6001 ctggggacaa cgctttattg aagtatggtc ggggatgccg acgctgtttt tgattatttt
     6061 actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattactg tcttgtttgg
     6121 ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtactcgta atttcgacta
     6181 cattcgtgcg gcacaggcgc ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt
     6241 gcctaatgcc atggtcgcga ccctcacctt tttaccgttt attttatgta gttcgataac
     6301 caccctgacc tcgctcgatt tcctcggctt cggtctaccg ctcggttcac cgtcactcgg
     6361 tgaactgctg ttacaaggga aaaataacct tcaggccccg tggcttggga tcaccgcctt
     6421 cttgtcggtg gcgatattat tgtctttgct gatctttatt ggtgaagccg tccgcgacgc
     6481 atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaaaatttgt
     6541 cggtgggttt tcgccatcag caaaccgtac gtacagtagt caatgatgtt tcactacaga
     6601 ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa agcgttaccg
     6661 cgctgtcaat tttacgcctg ctcccttccc cgccggttga atatctctcc ggcgatattc
     6721 gttttcatgg cgaatcgctg cttcatgtca gcgatcaaac gttgcgcggt gtacgcggta
     6781 ataagatcgc catgattttt caggaaccga tggtgtcgtt aaatccattg cataccctgg
     6841 aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg
     6901 gcgaaattct taactgcctt gatcgcgttg gtatccgcca ggcggcaaaa cggctgacag
     6961 attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt
     7021 taacgcgacc ggaattatta attgccgatg aaccgaccac cgcactggac gtctctgtcc
     7081 aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg ggcatgctgt
     7141 ttattactca taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa
     7201 acggtcgctg tgtcgagcaa aattacgccg ctacgctatt tgcatcaccc actcatcctt
     7261 acacacaaaa gctactcaac agtgaaccgt caggcgatcc agtgccgttg ccagaacctg
     7321 cctcaacgtt gctggatgtt gaacagcttc aggttgcctt ccccattcgc aaagggattt
     7381 tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgagcgg
     7441 gtgaaacact gggtttagtg ggcgagtccg gttccgggaa aagtacgacg ggactggcgc
     7501 tgctgcgact gattaattct cagggcagca tcatctttga cggtcagcca ctgcaaaatt
     7561 taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt caggatccaa
     7621 actcctcgct caacccacga ctcaacgttt tgcagattat tgaggaaggc ttacgggttc
     7681 accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg atgcatgaag
     7741 tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcgac
     7801 aacgtattgc gattgccagg gcattaattc ttaagccctc gctgatcata cttgatgaac
     7861 cgacatcatc actcgacaaa acggtacagg cgcaaatatt gaggctattg aaatcattgc
     7921 aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt gtccgcgcgt
     7981 tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc
     8041 gcgtgtttgc cacaccgcag caggagtata cgcgtcagct actggcgttg agctgacgct
     8101 taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg
     8161 catgttgcag caaactcgtc ctggcgattt acaaacaggc acggctcacc ttcacactcc
     8221 agcaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttata catcaccgac
     8281 catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac
     8341 ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag
     8401 ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag
     8461 cacattcact tcacaatttg tttacatcaa ttttaacaca tcatcaacaa tccgtcttta
     8521 tacagatcgt aaagattgat gcctcgcctg cgtggcctct atggctctga tttaagtaat
     8581 atctgcgggc gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tct
//
LOCUS       DAPPNJ010000179         8630 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000179 DAPPNJ010000000
VERSION     DAPPNJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.181"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            197..894
                     /locus_tag="RPM73_003599"
                     /pseudo
     CDS             197..894
                     /locus_tag="RPM73_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1082..2155)
                     /locus_tag="RPM73_003600"
     CDS             complement(1082..2155)
                     /locus_tag="RPM73_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX6521918.1"
                     /translation="MATIRKRGNYQWEAQIRKRGFPSQTKTFNTKVEAEAWAKMIESE
                     MARGVWLSRSEAESTTLYEALTRYEKEIVPDKKGAVQDRSLVRILKGTQLAKNYMASI
                     RSADVAKLRDEWLKIYAPATVLRRLALLSHVFNVSRKEWGMESLLNPVEAIRKPQPKN
                     SRTRRLEALPVVSEKTGVTSGKEIANEIEHIIAATHSLVLPAIILLALETAMRRSEIA
                     ELRWRFIDLDRRVAHLPDTKNGNARDVPLSTKAITILSSLKEHSKPAADKVFDMRADA
                     ITRAFDRAVKRARERYEKTNSLCDESFLKDLRFHDLRHEATSRLAEIFPMHELTKITG
                     HKDPRMLMRYYHPKAEDLALKLK"
     gene            2181..2292
                     /locus_tag="RPM73_003601"
     tRNA            2181..2292
                     /locus_tag="RPM73_003601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2272..2274,aa:Ser,seq:cga)
     gene            complement(2419..2757)
                     /locus_tag="RPM73_003602"
                     /pseudo
     CDS             complement(2419..2757)
                     /locus_tag="RPM73_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2808..3764)
                     /gene="paoD"
                     /locus_tag="RPM73_003603"
     CDS             complement(2808..3764)
                     /gene="paoD"
                     /locus_tag="RPM73_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDX6521919.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHQEEDSCLPPSS"
     gene            complement(3774..5972)
                     /gene="paoC"
                     /locus_tag="RPM73_003604"
     CDS             complement(3774..5972)
                     /gene="paoC"
                     /locus_tag="RPM73_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDX6521920.1"
                     /translation="MKFDKPAGENTIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAANGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(5969..6925)
                     /gene="paoB"
                     /locus_tag="RPM73_003605"
     CDS             complement(5969..6925)
                     /gene="paoB"
                     /locus_tag="RPM73_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDX6521921.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(6922..7611)
                     /gene="paoA"
                     /locus_tag="RPM73_003606"
     CDS             complement(6922..7611)
                     /gene="paoA"
                     /locus_tag="RPM73_003606"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDX6521922.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILT
                     AIEDAAGEIKS"
     gene            8029..>8630
                     /locus_tag="RPM73_003607"
     CDS             8029..>8630
                     /locus_tag="RPM73_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDX6521923.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLG"
BASE COUNT         1887 a         2277 c         2307 g         2159 t
ORIGIN      
        1 ccatgcaccg tccctattaa tctgcaacga tacattactg tgattattgg gttcaattcg
       61 aaaaaaaatg agatttcttc aggatattcc gaatctgagt cactacaata ctgcttctga
      121 gtcttatgaa tgagataaat gggtaatgct gccaacttac tgatttagtg tatgatggtg
      181 attttaaggt gcttgcgtgg cttccatttc catcagatgt ccttcctgct ccgctactga
      241 aggcgtggtg cgtaacggca aaagcactgc cgggcatcag cgctatctct gctctcattg
      301 ccgtaaaaca tggcaactac agttcactta caccgcctct cagcccggta cgcaccagaa
      361 aatcattgat atggccatga atggcgtcgg atgtcgcgcc agtgcacgca ttatgggcgt
      421 tggcctcaac acggttttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
      481 atacaaccgg gcagtgatgt gattgtctgc gctgaaatgg acgaacagtg gggctacgtc
      541 ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg acaggatacg gaggacggtt
      601 gtggcgcacg tcttcggtga acgcactctg gccacactgg agtgtcttct gagcctgctg
      661 tcggcctttg aggtcgtggt atggatgacg gatggctggc cgctgtatga atcccgcctg
      721 aagggaaagc tgcacgttat cagcaagcgt tacactcagc gcattgagcg acataacctg
      781 aatctgagac aacatctggc aaggctggga cggaagtcac tgtcgttctc aaaatcggtg
      841 gagctgcatg acaaggtcat cgggcattat ctgaacataa aacactatca gtaagttgga
      901 gtcattacca agctatagat gatagaaaaa ttggagcggc aatgtatata ttttctagcc
      961 ataatgggtt ttctacaatc aagtatccaa aaggtattaa gtatattata gccaatagtt
     1021 gaaggttttt taatttggat gatgcgtcaa ccaacttatt tttatattcc atacaacgtc
     1081 cttatttcaa ctttaacgcc aaatcttcag cttttggatg ataataccgc attagcattc
     1141 ttggatcttt atgtcctgta attttggtca gttcatgcat agggaaaatt tcagcaagtc
     1201 gagaagttgc ttcatgtcga aggtcatgaa atcttaaatc tttaaggaat gattcatcac
     1261 ataatgagtt tgttttctcg tatcgttctc tggctcgttt tacagctcgg tcaaaagctc
     1321 gggtaatcgc gtctgcacgc atatcaaaaa ccttatcagc agctggctta gaatgttcct
     1381 tgagactgga cagtatcgtg atggctttcg ttgataaagg gacatcgcgg gcattaccat
     1441 tcttagtatc cggcagatgt gcaactcgcc gatcaagatc gataaagcgc catcgaagct
     1501 ctgcgatttc gctacggcgc atggctgtct ctaacgccag aagaatgatt gctggcaata
     1561 ctaatgaatg tgttgcggcg atgatatgtt cgatctcgtt ggctatttcc tttcctgacg
     1621 taacaccggt tttctcagaa actacaggca gcgcttccag gcgtctggtc ctggagttct
     1681 tgggttgagg tttgcgaatg gcctctacgg ggttcagcag gctctccatg ccccattctt
     1741 tccgcgaaac gttgaataca tgagaaagta aggccaaacg ccttaaaacg gttgcagggg
     1801 catagatttt caaccattca tctctcagct tggcgacatc ggcactcctg atgctggcca
     1861 tatagttttt cgccagctga gtgcctttta gtatccgcac caatgatcgg tcttgcactg
     1921 cccctttttt gtcgggaaca atctcttttt catatcgcgt tagggcttca tacaatgtcg
     1981 tagattccgc ttcgctgcgg ctaagccaaa ctcctctcgc catttctgac tcgatcattt
     2041 tggcccaagc ttctgcttct actttggtgt taaaggtttt ggtttgagag ggaaaacctc
     2101 gtttacggat ttgagcttcc cattggtagt taccacgttt gcgtatagtc gccatttctg
     2161 tctgattcct aacgaactgt ggcaatattg tggcaaaaaa tccatttaaa ttcgataggt
     2221 attgatttta caagatcaat ttacgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     2281 ttatcggcac cattaaaatc aaagagttac cccatattta aatacaccac gtttcctcct
     2341 gtgccgcatt tgtgccattg taaccttggc aattcatcaa aatactgttc tgacatcagg
     2401 cagtgcaggt gcagctattt aagccaattg ctgccgccat tctttgacgt agtcaatcag
     2461 ggtgcggagc tttggtgcaa tattgcgacg ctgtgggaaa tacagataga agcccggaaa
     2521 ttgtggaaga aagtcatcaa gcagcgatac aagcttaccg ctttcaatat atggcctgaa
     2581 agtttcctga gtggcaattg ttattcctcc gccggcaaga accagcctca acatcagacg
     2641 cagatcatta gtcgtaatct gcggttcaat cgcaaggtcg aaagttctcc cgttttcttc
     2701 aaatggccag cgataaggcg caacctccgg ggactgacgc cagccgatac acttatgggt
     2761 atttccctcg gaggcgagaa agcgctttcc acgcccggcc gcaaggatca ggacgacggg
     2821 ggcaggcatg aatcctcctc ctgatggaga cgtacagagg cgatttctgc cagcacggag
     2881 agtgccagag tatgcgcatc ccgggctttg gggaatatcc cgacgggggc ccggatttgc
     2941 gctgtctcct ccctggacca tcccagctcg tggagctttt gcagacgtaa cgtgtgggtt
     3001 cgatggctgc ccaatgcgcc gagataaaag ggttttgctt ctcgcgcggc ctgcaacact
     3061 ggcagctccc ggttgagatc atggcacagc aaaatgaccg ccgtatcggt atcgatctga
     3121 gcgctggctg aggccggaaa aagatcgaag atatggctgt catagcctgt ggctgctgca
     3181 agactcgcgg ttgcctgcgc ctcaagagaa cgtccgtaaa tcatcagcct gacgcatggc
     3241 ctgaacccca cctcaaagcc attgagattc cagcccgttc gggtttgcgt gggcaggcac
     3301 accaacgatt gtgcttgcgg atcgtagcgc agccccgccg gttttctctg ttccaggcgg
     3361 ttcagcacgg cgagcagagg ctgtgccgag cgtagtttat ggagcgtcag cgtgatccca
     3421 ccgccgcagg gcagaacgat gtcaaaccac ggcgaacctt cgccatagcg aatttcgcga
     3481 tcgcggcctg agcccatcat ctccagcgct tcaaaagcgg cagcggcctc cacgcagccg
     3541 ccagagacaa aaccgcagta acgaccatct tcgcgcacca ccatctgcgc cccgagcggg
     3601 cgcgccgcgc cgccgcgtat ctccaccagc gtcaccagca ccgctccttt accggacatt
     3661 agcgcctcta cggcgaagcg cagaatggtc cggtggtcat cggtgagaaa cgcctgctct
     3721 ggcttatgcc agtgttcgtc tttatcaaaa agcgggtatg acattgtttc tccttaaacc
     3781 acatccggca gcttatcgag cagcttatcc agagtgatgg gataatcgcg tacccgaata
     3841 ccggtggcgt tatacaccgc gttggcgata gccgcgctca cgccgcacag gcccagctca
     3901 ccgacaccct tggccttcat cggggaggat atggggtcgg tatcatccag gaaaatcacc
     3961 tcctgttttg ggatatccgc atggaccggc acctcatacc ccgccatatc gtgattaacg
     4021 aagtagccca aacggtcatc caccgccagc tcctccatca gtgccgcgcc catgcccata
     4081 gtcattgcgc caatgacctg gctgcgcgca gttttcggat tcaggatgcg tcctgcagca
     4141 cacacagcga gcatacgccg gacccgaact tctcccgtcg cgctatgcac gccgacctcc
     4201 acaaaatgcc cggcaaaggt cgactgctgg tactctttgc tcagtgttcc gaattcaatg
     4261 ctctcttccg ctgtcagtct gccgcctgcg gtggcttcat gtagcgtggc gcttcgggta
     4321 ccgttggtaa tcttgccgtc ggcaaactgc gactgctcag gatcaaaccc gactgccgag
     4381 gcaatcattt cgcgaagctt catacaggcg gcgtaaacgc cggaggtgga ggtattcgcg
     4441 ccccattgtc caccagaacc cgcagaaacc gggaaactgg aatcgccgag gtgaaccgca
     4501 acctgctcca gcggtacgcc aagcatttca gctgccgtct gggccagaat ggtgtagctg
     4561 ccggtgccaa tgtcggtcat gtccgtttct acggtaacgg tgccgttttg ttcgaggtga
     4621 acccgagcac ccgatttttc cagcagatta ttgcgaaagc ccgccgcaac accgtggccg
     4681 actagccact ccccgtcgcg cacctgtccg ggtgtggcgt tgcgctgctt ccagccaaat
     4741 ttatccgctc cggtgcgcaa gcactcgata agctgacggc gagagaagca gcgcgtcggg
     4801 tcggcggggt caacctgagt gtcattcagg atgcgaaact cgacgggatc gatgcccgct
     4861 ttttccgcca gttcgtcgat cgcgatttcg agcgccatca gaccgggggc ttcgccgggc
     4921 gcacgcatgg cgttcccttc cggcaaatca agcgtggcga gccgcaggcc ggtatgacga
     4981 ttcgccccgg cgtagagtaa ttcgctttgc tgtaccgccg tttccggcgt gccgccgggc
     5041 aggtttccag accagctttc atgtgagata gcggtgattt tcccgctctg gtcggcaccg
     5101 atacgcaagt gctgaagggt ggcggggcgg tgcgtggtgt tattgggaat agaggggcgg
     5161 gggagcatca ctttaaccgg acgtttcacc gctcgggcgg cgagggccgc cagcagcgca
     5221 tcgcttctca ggaacagctt gccgccaaac cctccgccga tatacgggga gataatacgc
     5281 acattctcca cgggaacttt cagcgttttt gccagatcgg tgcggcacca gtcaatcatc
     5341 tgatttgagg tccagagggt aagcttattt ccatcccaga cggccatcga ggcatgcggc
     5401 tccatcgcca tatggctctg gtccggggtc gtgtaggtag catcaatctt caccgcagcg
     5461 gaggtgaaag ccccgtcaaa gtcaccgacg tttttgtcgg gcgtgtcttc cggcggctga
     5521 ttgacggcct gtttttcgtc cgccagggag taagctcctt tattacggcg atagtgtgcc
     5581 tgcaccagcg aggccgccgc tcgcgcctgt tcgaaggtct cggccactac cagcgcaatg
     5641 gcctgatgat agtgctcaat agtggggccg cctaacagtc tggcggtgtt tttgtcgcct
     5701 ttgccgagtg ccccggcgtt actggcggta atgacagcca gtacgcccgg cgctttttgc
     5761 gcggcgtccg tatcaagggc ggtgaggcgt cctttggcaa tggcggaacc gacgatatag
     5821 ccattggcgg cgttgggggc ttcttcatgc cattcgtagg cgtagcgtgc cgtgccggta
     5881 gttttcagcg gtccgtcgat gcggtcatgg ggacgaccga caaccttcag ctgatcgatc
     5941 gtgttttccc ctgcgggttt atcaaatttc atgcctgtgc cctcgcttca gccagtacgg
     6001 aggcaagcgt tcgcttcgcc aacaggagtt taaaggtgtt ttcagcggtg ggatgggcgc
     6061 tggcgaacag cgtgtcatat acggcctgcg ccccctggga tagctgagca tccgcagcct
     6121 caatgcgcca gggcttatgt gctactccgc ccagcgcgac gcgcccgctg ccgtcagggt
     6181 gaataatcgc cgcgaccgat accagggcaa aggcgtagga ggcgcgatcg cgcaccttac
     6241 ggtagatatg ttttccgccg agcggtggag gtaacgtcac cgcaacgata agctcaccgg
     6301 gaagcagggc ggtttcaatg tgcggcgttt tccccggtgg gtgataaaaa tcagccagtg
     6361 tgatactgcg agtctttccc tccggcgtga tggtttccac caccgcatcc agcaaccgca
     6421 ttgcgaccgc catatcgctc ggatgggtgg ctatgcaggc ttcacttacg cctaccaccg
     6481 cgtgctgacg gctaaagcct tcaagcgccg cgcagccgct cccgggcagg cgcttattgc
     6541 agggctgatt ggtgtcgtaa aaataggggc agcgcgtgcg ctggagcaga ttacctgcgg
     6601 tggttgcctg attacgtaac tgaccagatg cgccagcgag cagggcgcgg gagagtaccg
     6661 cgtaatcacg acgcacgcgc tcgtgagccg ccaggtcggt gttccgtacc agtgcgccga
     6721 tgcgcagccc acccgcgtcg gtcacttcaa tcttatcgag cccgaggccg ttcacatcga
     6781 taaggtgggt gggcgtttca atttccagct tcatcaggtc cagcagattg gtcccgcccg
     6841 cgataaattt tgcgccgggt acgcgctgag cgctaagtgc cgcctcggct ggggtgttca
     6901 ctcgttcata ggtaaacgcc ttcatgattt tatctccccc gcagcatctt caatggcggt
     6961 aaggatgtta gcgtatgcac cacagcgaca gatgttgccg ctcatacgtt cacggatctc
     7021 atcggcagtt gtttctggag cggaaaccaa atcgaccgtg acgtgactgg gaatgccgtc
     7081 ctgaatctct tttagcaccg ctactgatga gcaaatttgc ccggaggtgc agtagccgca
     7141 ctggaagcca tcatgcttga taaaggccgc ctgcatgggg tgaagattat ctggcgaacc
     7201 caggccttca atggtggtga tctcggcccc ctgatgcatg actgcaagcg tcaggcaggc
     7261 attaagcctg cgaccattga ccagcacggt acaggctccg cactgtccgt gatcgcaacc
     7321 tttcttggta ccgatcaaat gcagattttc acgcaaagcg tccagtagcg tggttcgggt
     7381 atccacctca agctgctcgg ttttgccgtt caccttcagt gtcaggggca ttatctctgg
     7441 cgcgggtgta gctgccggaa cgcttgccgc cagcgtagaa tgaggataaa ccacggcggt
     7501 cgccgctgtt gcggcgctca ctttaatcag atcgcgacgg gtcaaactta aatcgtgcgg
     7561 ctcgtgcttc ccaacccgat tatcttcggg gtattcgcct tggttgctca taccaggcct
     7621 ccggtattct aaaggggaaa ataagagtgc gttgcgagag tgctgatgct tttttaaacg
     7681 ttaagcatag tcggcgacag caggaggatt attcttaaaa cgcgaatgca gctatgagcc
     7741 cggctaataa attcatgttc gccgggatgt tgatgatgat gggagctggt ttattgcttt
     7801 agttgtacga tgcaaaaacc aataaggaaa cctgtgattt tcagctctgc atcaccctgc
     7861 aaatctctgt cacttctaat ataaaaatag ggagaaatga tggagcttat attcattggc
     7921 gattaggaaa ctatcttgtt atacaaaaca atacagttct ttacatttgc cttgttttat
     7981 gaatactcct gaagaggtgt ataacataat ggtacaagca gggtagatat gaatatattt
     8041 gaacaaactc caccgaaccg cagacgttat ggtcttgctg cattcattgg gctgattgct
     8101 ggcgttgttt ccgcattcgt gaagtggggg gctgaagttc cattgccgcc acgtagcccg
     8161 gtggatatgt ttaatgcagc gtgtggcccg gaatcattaa tcagggctgc aggccaaatt
     8221 gattgctcgc gtaattttct caatccaccg tatatttttc ttcgagactg gttggggctg
     8281 acagatccca atgcggctgt ttataccttt gccgggcatg tctttaactg ggttggtgtt
     8341 acgcacatta tcttttcgat agtgtttgct gtcggttatt gtgtggtcgc tgaagtattt
     8401 ccaaaaatta aactctggca gggcttactg gcaggtgctt tagcccaact ttttgttcat
     8461 atgatttcat tccctctcat gggactgacg ccacctctgt ttgatctccc gtggtatgag
     8521 aatgtttctg aaatttttgg acatttagtc tggttctggt ctattgaaat tattcgcaga
     8581 gatttacgaa acagaattac tcatgagcca gaccctgaga tccctttagg
//
LOCUS       DAPPNJ010000180         8558 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000180 DAPPNJ010000000
VERSION     DAPPNJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.182"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            110..2488
                     /gene="ppsA"
                     /locus_tag="RPM73_003608"
     CDS             110..2488
                     /gene="ppsA"
                     /locus_tag="RPM73_003608"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDX6521924.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="RPM73_003609"
     CDS             complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="RPM73_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDX6521925.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4248..4541)
                     /locus_tag="RPM73_003610"
     CDS             complement(4248..4541)
                     /locus_tag="RPM73_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDX6521926.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4538..5827)
                     /locus_tag="RPM73_003611"
     CDS             complement(4538..5827)
                     /locus_tag="RPM73_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX6521927.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALIENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5883..6821)
                     /locus_tag="RPM73_003612"
     CDS             complement(5883..6821)
                     /locus_tag="RPM73_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDX6521928.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6841..7605)
                     /locus_tag="RPM73_003613"
     CDS             complement(6841..7605)
                     /locus_tag="RPM73_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDX6521929.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7921..>8558
                     /locus_tag="RPM73_003614"
     CDS             7921..>8558
                     /locus_tag="RPM73_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDX6521930.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSY"
BASE COUNT         2061 a         2191 c         2205 g         2101 t
ORIGIN      
        1 tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc
       61 gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa
      121 tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt
      181 tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc
      241 cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg
      301 cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg ttactcagct
      361 tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct
      421 ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcgtt
      481 tgcggtgcgt tcctccgcta ctgcagaaga tatgccggac gcttcttttg ccggtcagca
      541 ggaaaccttc ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt
      601 tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg
      661 tggcgtggcg ctctccgccg gtgttcagcg gatggtgcgc tccgacctcg catcttctgg
      721 cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc
      781 atggggcctt ggggaaatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtcca
      841 taaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa
      901 aatccgcatg gtttatgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt
      961 accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa
     1021 acaggccgta caaattgaga aacactacgg tcgcccaatg gatattgagt gggcgaaaga
     1081 tggccacacc ggcaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg
     1141 tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc
     1201 tatcggtcat cgcatcggtg cgggtccggt gaaagtcatc catgacatca gcgaaatgaa
     1261 ccgcatcgaa cctggtgacg tgctggtcac tgatatgacc gacccggact gggaaccgat
     1321 catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat
     1381 catcgctcgt gaactgggca ttccggcggt agtgggctgt ggagatgcaa cagaacggat
     1441 gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc
     1501 ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt
     1561 gaaagtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gtctgccgaa
     1621 cgaaggcgtg ggccttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc
     1681 acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat
     1741 gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc
     1801 gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc
     1861 gaacgaatat gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat
     1921 gctcggcttc cgtggcgcgg gccgctatgt ttccgacagc ttccgcgact gtttcgcgct
     1981 ggagtgtgaa gcagtgaaac gtgtgcgcaa tgacatgggg ctgaccaacg ttgagatcat
     2041 gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgaagaac tggcgcgtca
     2101 ggggctgaaa cgtggcgaga acgggctgaa aatcatcatg atgtgtgaaa tcccgtccaa
     2161 cgccttgctg gcagagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga
     2221 catgacacag ctggcgctcg gtctggaccg tgactccgga gtggtgtctg aactgttcga
     2281 tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg cgaagaaaca
     2341 gggcaaatat gtcgggattt gcggtcaggg tccgtccgac catgaagact ttgccgcatg
     2401 gttgatggaa gaggggatcg atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg
     2461 gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgttca gtcgccgggt
     2521 ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct gcgttaatcg
     2581 ccgcataata tcttttctta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag
     2641 tttttcgatt accacgatat gttcaggata tttatatttt gcgacccgtt tacggctaaa
     2701 aaaagccacc acctcttcca gcgataatga atgatgcggc gctttcagca cgacataagc
     2761 gcatgatcgt tcacctaaac gttcatcggg cattgcaacc acacaggcat cgtgaatttt
     2821 aggatgctgc aataaaatat cttccacttc acggctgcta atattttcgc cgccgcggac
     2881 aataatatct tttttgcgtc cggtaatttt tatatagcca gcctcatcca tacggcagag
     2941 atcgccgctg taataccagc cttcttcatc cagcgcacgg gcggttaatt caggttcatc
     3001 aaaatatccc ataaacacat tggggccacg cgaggcttct tcaccttcgc aacctggcgg
     3061 taaggtcttg cgtgcgtcat cgaccacttt aatctctaca cctgcggcag cgtaaccatc
     3121 ggtgtgcata aagcgcgaca aaggatcatc gagattcacc accgcatgcg gcgaactttc
     3181 tgtggaacca taaacactta ataatttaat gccgcgctgc tggcattcac gcgccacttt
     3241 tttggggatt gtggtaccgc cgcaaagaaa gaagcgcagc gctgaaaggt ctgcgggttg
     3301 tttctctagt aaattcaaaa gatcatagac aaacggcgtt gcgccgagca tacaggtgca
     3361 acgctgctgc tcaagcagcg cgagacacgc atcaggagtg aaaatatcta acaacacgct
     3421 gcgagcacca atcaaaaatg gtgccgttac gccatgcaga aagcccgttg cgtggccgag
     3481 tggcgcaggc atcataaaga catcctgcca ggtcagattc agccgcgcgc aataagcccg
     3541 ctcactggcg agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc
     3601 ggaggtaaac agcaccgcag ctaattcatc gccgtgggtc gttatcgccg tggtcagtgg
     3661 ggtattgtcg gcgataatct gacttaatga gagggaagag gtggcgggag ccagtttgtc
     3721 cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac
     3781 cggacgcgtt tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag
     3841 cacccatacc agttctgctt cccgccagga aggcaacagc ggtacggaaa ccgcaccgat
     3901 tttcaggcat gcaagataga taacagtaaa ttcacaccag ccaggcagtt gaaatgcgat
     3961 gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg
     4021 atcgagcgcg ctataggtgt acgatgctcc atgattatcg accacggcaa ttttgtctgg
     4081 catcgcacga gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc atagcccttg
     4141 ctgacgatac gccgcacgac gttgttcgtt gaacgttaat gtcactttca tgttggactc
     4201 tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga
     4261 aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttaccgcac
     4321 agcacccgac aggtgccgca ctccagacaa ccggcagaat caaaatgaat gttgccggcg
     4381 tcatcctgct tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata
     4441 tcgggatttg ccgccagaat gatatgcggg tggccctcat caacatggaa tttattgacg
     4501 cctaatttga tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa
     4561 tgccatcttt cagcaagttg atcagcccaa ttttcttcgc atgtcccatg atcattttgc
     4621 gtaccggctg gtttggtttg ccgtcaatgg tgaacatctc gttcatgatg tcggcgacca
     4681 ttcgtgggta ttggctaaac aggcgcgggt tttctatcag cgccgggatc ttgcggaaat
     4741 gctgcatatc gcgcatgacg cagctttgtt ccagctcacg tttgtattgc gccagactgc
     4801 tggcggagaa atcctcgcgt tctttggcgg cgatcaccgt tgtggcggca gcctgagccg
     4861 atgcaatggc taaatccatg ccacgaactg taaaacccaa attcaggcag aagcctgcgg
     4921 cgtcaccaac gatcatcacg ccatcgttaa ccatctgcgg caccattgcc agaccgcctt
     4981 ctggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaattagt gggcgaatgg
     5041 cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca tgggcgatat
     5101 cacccaatcc acaaaccagc cccaaggata tggaatcctt gttggtatag agaaatcccc
     5161 cgcccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg
     5221 taatattaaa gcgatcgttg atctgttctg gtgtgaggcc aataacctct ttaacaccaa
     5281 cagcgtaatg atgcggatcg gaagcgggaa ccattcccag cgagcggcca agcatcgagt
     5341 taacgccatc agccagaatc accacattcg cttcgagaat atcatcccca gcctgcacgc
     5401 cagtgacctt gtttccttca cgaaccagcg catcgacgcg aacgcctggg ataaactgcg
     5461 cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat
     5521 aagatgcgtg ttgcggaaca tctggttgct cgcggtgaaa atcgagggta acggcgcttt
     5581 cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatactg
     5641 caaagcctgg aatgattgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt
     5701 tacatccggc actgtcgccg cgttctatca ccaggacatc cagcccggct cgcgccatga
     5761 ccagtgcggc aacgctacca gcaacgcccg caccgaccac aatggcatca aatttgtctt
     5821 ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg
     5881 aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc gcccaccaga
     5941 ccgtagtcgg cataattgaa gattggcgca tttttatctt tattgatggc gacaatcact
     6001 tttgcgccgt tgccgccaac catatgctgg atctgcccgg agatccccag cgtcaggtag
     6061 agatcggatt tcagcaacac gccggagaca ccgatataac gttcacgctc catccagttc
     6121 tcgccttcgg caattggacg tgaacagccg acttcagcat tcagcaccgc cgccagttcg
     6181 tggaccattt tcaggtcatc ctgcgccgcc agaccacgac cgacgccaac cacacgtttt
     6241 gctttgctca ggtccacgct gcttgcggct ttcgcacggc gttcgcgaca gagaatttca
     6301 tgacgcggag caacataagg taccgtttct gtcgggcact gatgagaggt atcactggtg
     6361 cacggttcct gaacaccggg tgcaagggta atgatcgcca gcgggctgtt gatcttttcc
     6421 tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc atcgacaata
     6481 tccaccgccg tggcatcatt caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc
     6541 agagctttac cacgtttcgt cgcggccaac agcaacatag cggggtgttt atctttcagc
     6601 agggcagcaa tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga
     6661 acataaatac attttggacc ataaggcata actgcctgcg cctggtcggt attttgtaca
     6721 atggcataca cctgttggcc ccattgctga gcgccgccaa acagttcagc ataacgttca
     6781 ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc tccgtaggct
     6841 tcagttcagg gctttcttca gatgttccgc cagctcggca atggcctccg gcgaatcgtt
     6901 atcgataatg atgtgcttac gttctgtttg cggcggtacg cgaatgccga ccagttcagc
     6961 aagtggcgcg ctctggctcc agtcaatatc acttgcctgc cactgattta ccggtttttt
     7021 acccgcgccg agaatggctt tcatcgaagg aatgcgtggc acgttaatat cggaggtgac
     7081 gcagagcacg gctggaacag agagttcaat aacttcaaca tcatcttcaa gcgtgcgttc
     7141 aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg
     7201 cagaatttct ccgaccagca agccagcctg ctgggcataa aggtcgccgg aaccttcacc
     7261 aaagatcagt aaatcgaagc cgatcttttc aactgctgct gccagcgctt ttgcggtatc
     7321 gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg
     7381 ggatagcacg tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc
     7441 ggctatctca tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa
     7501 ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat
     7561 gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag
     7621 tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc
     7681 ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
     7741 cgatcgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
     7801 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
     7861 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
     7921 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
     7981 cggctttcac gttttgcaat tagcgatgat ccaaaatggg agtctggaca tcacgttcat
     8041 gacaatgaaa ccgagctgat ttacgtcaag aaaggggtag caaggtttac catcgattca
     8101 tcgttatatg tcgcacatgc cgatgatata gtggtgatag aacgcggcag gctgcatgcg
     8161 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
     8221 tttcaggggg ctgaggaaaa tcagctactg caaccgcatt cttgtccggt aattgccgcg
     8281 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
     8341 agtaaaaata gccaaacatc ttcgttatgg gatgcatttg cctatacgtt agccattctt
     8401 tactacgaaa actttaaaaa tgcttatcgt tcggagcagg ggtatattaa aaaagatgtt
     8461 ctgataaaag atattctttt ttatctgaat aataattatc gcgaaaaaat cactttagaa
     8521 cagttatcga aaaaatttcg tgccagcgtc agttatat
//
LOCUS       DAPPNJ010000181         8537 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000181 DAPPNJ010000000
VERSION     DAPPNJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.183"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..672)
                     /gene="dsbG"
                     /locus_tag="RPM73_003615"
     CDS             complement(<1..672)
                     /gene="dsbG"
                     /locus_tag="RPM73_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDX6521931.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFTDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMS"
     gene            1044..1607
                     /gene="ahpC"
                     /locus_tag="RPM73_003616"
     CDS             1044..1607
                     /gene="ahpC"
                     /locus_tag="RPM73_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDX6521932.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1736..3301
                     /gene="ahpF"
                     /locus_tag="RPM73_003617"
     CDS             1736..3301
                     /gene="ahpF"
                     /locus_tag="RPM73_003617"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDX6521933.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3422..3850)
                     /gene="uspG"
                     /locus_tag="RPM73_003618"
     CDS             complement(3422..3850)
                     /gene="uspG"
                     /locus_tag="RPM73_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDX6521934.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4071..5309
                     /locus_tag="RPM73_003619"
     CDS             4071..5309
                     /locus_tag="RPM73_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDX6521935.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5540..5950)
                     /gene="rnk"
                     /locus_tag="RPM73_003620"
     CDS             complement(5540..5950)
                     /gene="rnk"
                     /locus_tag="RPM73_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDX6521936.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6180..6986)
                     /gene="rna"
                     /locus_tag="RPM73_003621"
     CDS             complement(6180..6986)
                     /gene="rna"
                     /locus_tag="RPM73_003621"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDX6521937.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7100..>8537)
                     /gene="citT"
                     /locus_tag="RPM73_003622"
     CDS             complement(7100..>8537)
                     /gene="citT"
                     /locus_tag="RPM73_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDX6521938.1"
                     /translation="KLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPI
                     PATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAF
                     IFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFP
                     VIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIA
                     GIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTR
                     REWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLV
                     NLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAH
                     TATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKD
                     YWRLGAIFGVIYISMLLLVGWPILAMWN"
BASE COUNT         2224 a         2093 c         2185 g         2035 t
ORIGIN      
        1 actcatgtaa tagatagctg gtgtgacatt tgcccccaga tcgtccatga gtttctcgtt
       61 ggcgcttaac actttcattt gttctgtact tacgtttgca ggcacgttta gcttaagctt
      121 gccaccagag gattcatatt cttgccaggt ttttgcggga tctttggagg caagaattgc
      181 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      241 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      301 cggatcggtg aacacgtaga caatgaccgg cgcatctttt ttaccgtcga ggagccagtg
      361 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      421 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      481 accatcggga gtcaggtaga tggtgacgcc catatcctga tactttccga gataaccttt
      541 cattcctccg ggggcatcga acgttttgat gattgtaatg ccctgttttt caatcgcttt
      601 tactggggaa ggaagttcct ctgcgaaggc gattgcagga agcagagcca gtaaaagtat
      661 cttttttaac attaatttgt ccttttcagt cagtgcaaaa gtcgagtaaa aggcataacc
      721 tatcactgtc ataggtaaga gcttagatca ggtgattgcc ctttgtttat gagggtgttg
      781 taatccatgt cgttgttgca tttgtaaggg caacacctca gcctgcaggc aggcgctgaa
      841 gataccaaag ggtagttcag attacacggt cacctggaaa gggggccatt ttacttttta
      901 tcgccgctgg cggtgcaaag ttcacaaagt tgtcttacga aggttgtaag gtaaaactta
      961 tcgatttgat aatggaaacg cattagccga atcggcaaaa attggttacc ttacatctca
     1021 tcgaaaacac ggaggaagta tagatgtcct tgattaacac caaaattaaa ccttttaaaa
     1081 accaggcatt caaaaacggc gaattcatcg aaatcaccga aaaagatacc gaaggccgct
     1141 ggagcgtctt cttcttctac ccggctgact ttactttcgt atgcccgacc gaactgggtg
     1201 acgttgctga ccactacgaa gaactgcaga aactgggcgt agacgtatac gcagtatcta
     1261 ccgatactca cttcacccac aaagcatggc acagcagctc tgaaaccatc gctaaaatca
     1321 aatatgcgat gatcggcgac ccgactggcg ccctgacccg taacttcgac aacatgcgtg
     1381 aagatgaagg tctggctgac cgtgcgacct tcgttgttga cccgcagggt atcatccagg
     1441 caatcgaagt taccgctgaa ggcattggcc gtgacgcgtc tgacctgctg cgtaaaatca
     1501 aagcagcaca gtacgtagct tctcacccag gtgaagtttg cccggctaaa tggaaagaag
     1561 gtgaagcaac tctggctccg tctctggacc tggttggtaa aatctaagtt ccgcaatcgc
     1621 gtaattaatc gggtgctgcg gcacccgatt ttcttccccg caccatgatg caagctgcat
     1681 ccaggtagcc gcagaggccg cttgcatgat gatgtttaaa gcccaggaga taaacatgct
     1741 cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca agcctgttga
     1801 gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac tgttggctga
     1861 aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc cggtgcgtaa
     1921 gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg caggctcccc
     1981 gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg gtcatccgtc
     2041 gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt ttgaattcga
     2101 aacctattac tcgctctctt gccacaactg cccggacgtg gtgcaggcgc tgaacctgat
     2161 gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct tccagaacga
     2221 aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg
     2281 tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatactggcg cggaaaaacg
     2341 tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc
     2401 ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg gtctgatggg
     2461 cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca tttctgtacc
     2521 gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg aatacgacgt
     2581 tgatgtgatc gacagccaga gcgccagcaa actgatcccg gcagcggttg aaggtggtct
     2641 acaccagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta tcgtggcgac
     2701 cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt
     2761 gacctactgc ccgcactgcg acggcccgct gtttaaaggc aaacgcgtag cggttatcgg
     2821 cggcggtaac tccggcgtgg aagcggcaat cgacctggcg ggtatcgttg agcacgtaac
     2881 tctgctggaa tttgcgccag aaatgaaagc cgaccaggtg ctgcaggaca aactgcgcag
     2941 cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag
     3001 caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca acatcgaact
     3061 ggccggtatt ttcgtccaga ttggtctgct gccgaacacc aactggctgg aaggcgcagt
     3121 cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg
     3181 cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca tcgccactgg
     3241 cgaaggtgcc aaagcctctc tgagtgcttt tgactacctg attcgcacca aaactgcata
     3301 agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg gcggcttttt
     3361 tatggcatta aaaagccctg ccgggatggc tccgggcagg gcgggatatt tattctggca
     3421 attaacgcac aaccagcacc ggcagattgg catggcggat cacgctcgag gcgttagaac
     3481 ctaacagatg ggtcgaaatc gatgggttgc gagaaccaat aactacaaca tcagccccca
     3541 gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca tgttgtttaa
     3601 tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct tctgcttcat
     3661 gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg
     3721 ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg aggaattcag
     3781 cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc ataatgattg
     3841 tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca tgattataat
     3901 atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt gattaatata
     3961 ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg
     4021 atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc atgaaagcat
     4081 tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg ggcattgaac
     4141 aggcagatga tattattctg cgtattacgg caacggcgat ctgtggctct gacctccatc
     4201 tttatcgagg caaaataccc caggttaaac atggcgatat ttttggtcat gaatttatgg
     4261 gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa
     4321 ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg attacagcaa tatgccgcct
     4381 gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata ccagctcccg
     4441 cggcattgtt tggttatagt cacctgtatg gcggcgttcc tggtgggcag gcggaatatg
     4501 tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt tcagatgata
     4561 aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa aatgcgcaga
     4621 tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg ttgacaatcg
     4681 cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat ccctaccgct
     4741 tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa gatagcgatc
     4801 cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca gtaatagacg
     4861 ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac ctgaaactgg
     4921 agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg
     4981 ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc gacgcctttg
     5041 ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg ggagaattat
     5101 taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac tatatgccgt
     5161 ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg
     5221 tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg
     5281 tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag gtaaaggaca
     5341 gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa tgcttctgcc
     5401 atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca gtgagaacca
     5461 ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt
     5521 atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc tggtattcga
     5581 gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga atggaatcgc
     5641 caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc tgagtattgc
     5701 tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca tcgctaagat
     5761 tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct tctggcgaac
     5821 acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa ccagcatagg
     5881 cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg atgatagttg
     5941 gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa acatacccaa
     6001 aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac tgactgttgc
     6061 tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc gcattatgta
     6121 taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa atcagatgat
     6181 taataaccca ctttatcaat cacaaaggtt ttgccacagt tgcctgggtg tggttgtggc
     6241 aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga gatctgaatt
     6301 tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac gttctctttt
     6361 ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt accgtaatta
     6421 tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg taccatcgta
     6481 ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt
     6541 tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc ggccgtttcc
     6601 agtgataacc cggtttccgg cgatgaacac attcggctgg cgcgtgcttc tggtagattc
     6661 gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac accacgggca
     6721 gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc agctttgttg
     6781 gtcgtttcgg tttgcaggcg acattcatct cgttcgttac gatttcgatc gtgttgactc
     6841 tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc gccatactgt
     6901 tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac cgcgagcaac
     6961 gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac actgaatact
     7021 atctattaaa tcataaaaaa gcccgccagg tgggctaacc tggcgggcgt gatgatttat
     7081 tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca accaacagca
     7141 acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct tttgatttca
     7201 catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc agacagccca
     7261 tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg cccggaatac
     7321 ctttaccgac ggccagaata accggcagca tggttgcggt gtgcgcagac aggctggcaa
     7381 acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg tttggtgaga
     7441 atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca gaacgagtca
     7501 ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat gcgctgttat
     7561 agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa actgccagca
     7621 gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct aagctgagca
     7681 atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt ttcagttcat
     7741 cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac agcacgtagg
     7801 aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag aggaaccact
     7861 gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc agcacgtttg
     7921 gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc atcatccaca
     7981 tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta aacaacggcg
     8041 gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa
     8101 acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc
     8161 ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact tcatacccta
     8221 atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa ccagccagac
     8281 cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct ttggcatcaa
     8341 agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg atcgctgttg
     8401 ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg aagtaatgcc
     8461 atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc atcaccacca
     8521 gtggggccaa tagtttc
//
LOCUS       DAPPNJ010000182         8486 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000182 DAPPNJ010000000
VERSION     DAPPNJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.184"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(148..224)
                     /locus_tag="RPM73_003623"
     tRNA            complement(148..224)
                     /locus_tag="RPM73_003623"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Asp,seq:gtc)
     gene            complement(357..1088)
                     /gene="dnaQ"
                     /locus_tag="RPM73_003624"
     CDS             complement(357..1088)
                     /gene="dnaQ"
                     /locus_tag="RPM73_003624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDX6521939.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1153..1620
                     /gene="rnhA"
                     /locus_tag="RPM73_003625"
     CDS             1153..1620
                     /gene="rnhA"
                     /locus_tag="RPM73_003625"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDX6521940.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1617..2339)
                     /locus_tag="RPM73_003626"
     CDS             complement(1617..2339)
                     /locus_tag="RPM73_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDX6521941.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2373..3128
                     /gene="gloB"
                     /locus_tag="RPM73_003627"
     CDS             2373..3128
                     /gene="gloB"
                     /locus_tag="RPM73_003627"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDX6521942.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3200..4558
                     /gene="mltD"
                     /locus_tag="RPM73_003628"
     CDS             3200..4558
                     /gene="mltD"
                     /locus_tag="RPM73_003628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDX6521943.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4606..5376)
                     /locus_tag="RPM73_003629"
     CDS             complement(4606..5376)
                     /locus_tag="RPM73_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDX6521944.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DTSVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5454..6254)
                     /locus_tag="RPM73_003630"
     CDS             complement(5454..6254)
                     /locus_tag="RPM73_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDX6521945.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6495..7409
                     /locus_tag="RPM73_003631"
     CDS             6495..7409
                     /locus_tag="RPM73_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6521946.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7406..8208)
                     /gene="dkgB"
                     /locus_tag="RPM73_003632"
                     /pseudo
     CDS             complement(7406..8208)
                     /gene="dkgB"
                     /locus_tag="RPM73_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
     gene            complement(8371..8447)
                     /locus_tag="RPM73_003633"
     tRNA            complement(8371..8447)
                     /locus_tag="RPM73_003633"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8411..8413),aa:Asp,seq:gtc)
BASE COUNT         2260 a         2039 c         2060 g         2127 t
ORIGIN      
        1 gaattttaat gaatgagaca acaagcgaga atcacgcact tacgccagaa taaaatcatt
       61 gaacagagaa atggtggaat tcaaatacaa aaaaaccgca aaattaaaaa tcttgcggct
      121 ctctgaactc attttcatga gtgaatttgg cggaacggac gggactcgaa cccgcgaccc
      181 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccgcgttg tgttccgttg
      241 ggaacgaggc gaatattacg gattgcctca cccttcgtca acggtttttc tcatcttttg
      301 aatcgtttgc tgcaaaaatc gcccaagccg ctatttttag cgccttttac aggtatttat
      361 gcccgccaga ggcagcttcc gcccttcttc tccaccagat caagacgggc ttcatgagct
      421 gcaagctcct catctgtcgc aaaaacaacg cgtaacttac ttgcctgacg tacaatgcgc
      481 tgaattgttg cttcaccttg ttgctgctgt gtctctcctt ccatcgcaaa agccatcgac
      541 gtttgaccac cggtcatcgc cagataaact tccgcaagga tctgtgcatc gagtaatgcc
      601 ccgtgtagcg ttcgtttact gttatctatt tcgtagcggg cacataacgc atcgaggctg
      661 ttgcgcttac caggaaacat tttcctcgcc accgcaaggc tatcggtgac cttacagaag
      721 gtattggtct tcggaatatc gcgcttaagc aacgaaaact cgtagtccat aaagccgata
      781 tcgaacgctg cgttatggat caccaactcc gcgccgcgga tatagtccat gaactcatcg
      841 gctacttcgg caaacgtggg cttatcgagc aaaaattcat cggcaatacc atgtacgcca
      901 aaggcttccg gatccaccag ccgatcgggt ttgagataaa catggaagtt attgcccgtc
      961 aggcgacggt tcaccacttc aacggcacca atctcaatga tcttgtggcc ttcatagtgc
     1021 gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa cgatctggcg tgtaattgca
     1081 gtgctcatag cggtcattta tgtcagactt gtcgttttac agttcgattc aattacagga
     1141 agtctaccag agatgcttaa acaggtagaa attttcaccg atggttcgtg tctgggtaat
     1201 cctggacctg ggggttacgg cgctatttta cgctatcgcg gacgcgagaa aacctttagc
     1261 gcaggctaca cccgcaccac caacaaccgt atggagttga tggccgctat tgtcgcgctg
     1321 gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg acagccagta tgtccgccaa
     1381 ggtatcaccc agtggatcca taactggaaa aaacgtggct ggaaaacagc agacaaaaaa
     1441 ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg cattggggca gcatcaaatc
     1501 aaatgggaat gggttaaagg ccatgccgga cacccggaaa acgaacgctg tgatgaactg
     1561 gctcgtgctg cggcaatgaa tcccacactg gaagatacgg gctaccaagt cgaagtttaa
     1621 gcttgtggtt tacgacattg ccgggtggct ccaaccgcct ggcgaattcg tggcttgttt
     1681 ttactctgtt tcatcgggtt tagcgttaaa ggaatagtcc gtttccgggc aacaataagt
     1741 tgtaagcaac caagcgcagg aatatgcgca ttcaatagtt ttcctccgtg tttgttccac
     1801 gggagaacgt ggaaacggct ggcgtgcagc acttcaaaat tcaacaaaga gagccagtcc
     1861 agttgccgca tcagagtaaa catccggctg ttatagggcg aggtttttcg caataccggc
     1921 acaagtttgc gtaatcccat taaactaatg ggattgaagc cactaatcac cagccagcca
     1981 tcatcaatca atacccgatc ggtttcacgc aataaacgat gcggatcggt gcaccacggc
     2041 aatgtatgtg ccagtagaca aacatcaacg gatttatcgg caaaaggaag atgaagtgga
     2101 tccgcctgca cctggacggg cattccttgc gcagaaacat tcacttgatg agaaaccgcg
     2161 cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct taagcagatg aaaaccatac
     2221 attttagtga accacgggtt gagctggcgc tccagcgcct tgcgataaag ctctccccag
     2281 ggcaaatcgc cccagcgatc aggagccacg acagtttgag ggactcttgc cggtttcatc
     2341 acaaccttcc gtttcacact aagaggtaat ctatgaatct taacagtatt cccgcctttg
     2401 atgacaatta catctgggtt ttgaatgatg aagcaggtcg ctgcctgatt gtcgatcccg
     2461 gagacgcaga gccggtatta aacgccattg ccgccaataa ctggcaaccg gaggccatat
     2521 ttctcaccca ccatcatcac gatcacgttg gcggcgtaaa agaactggtg gaaaagtttc
     2581 cgcaaattgt ggtgtatggt ccacaagaga cacaagataa gggaacaaca caggtagtca
     2641 aagatggcga aactgccttc gttttggggc atgaatttag tgtaattgcc acaccgggtc
     2701 acactttagg acatatctgt tacttcagta aaccttatct attttgcggc gacacgctgt
     2761 tttctggtgg gtgtggtagg ttgtttgaag ggacagcatc acaaatgtat caatcactta
     2821 ataagttaag tgcgttacct gacgatacat tggtatgttg tgctcatgaa tatacgttat
     2881 caaatatgaa gtttgctttg agtattcttc cgcacgattt gtccataaat gattattatc
     2941 gtaaagttaa ggagttacgg gcaaaaaatc aaataacact acccgtaatt ctgaaaaatg
     3001 agcggcaaat taatgttttt ttaagaacgg aagatattga tttaattaat gtaattaatg
     3061 aagaaacatt attgcaacaa cctgaagagc gttttgcatg gttaaggtca aagaaagata
     3121 ggttctgata aaactttctt gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta
     3181 agcaactatt gacacacaca tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt
     3241 gggttgccag agtaccggca acgttcaaca gcacgcacag agcctttctg cagctggtca
     3301 aggggaagca gcaaagttta caagtcaggc acgatggatg gacgatggga cgtctatcgc
     3361 gccagatggt gacttgtggg ctttcattgg cgacgagcta aagatgggaa ttccggaaaa
     3421 tgaccggatt cgcgaacaga aacagaaata tttacgcaat aagagctatc tccacgatgt
     3481 aactttacgg gcagagccgt atatgtactg gatagccggg caagttaaaa aacgtaacat
     3541 gcctatggaa ctggtactac tacccatagt ggagagcgct tttgatcctc acgcaacgtc
     3601 tggcgccaat gccgcaggca tctggcagat cattccgagc acggggcgca attatggttt
     3661 gaaacagacc cgcaattatg acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct
     3721 gaatatgatg cagcgtctga acaaaatgtt tgacggcgac tggcttctga ccgtagcggc
     3781 ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa
     3841 atccacggac ttctggtcgt taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat
     3901 gctggcattg agtgatattc tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac
     3961 cgatgaaagc cgtgctctgg cgcgtgtgca cctgagcagc ccggttgaaa tggcgaaggt
     4021 tgcagatatg gcggggattt ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg
     4081 ctccacgctg ggcgcaagtg gtccgcaata cgtgatggtg ccaaagaagc atgcagatca
     4141 actgcgtgaa tctctcgctt caggcgaaat tgctgctgta cagtcgacgc tggttgccga
     4201 caatacgccg cttaacagcc gtgtttacac cgtacgctct ggcgacacgc tttcaagtat
     4261 cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc
     4321 taagctgaag ccaggccaaa gtttgacgat tggtgcaggc agtagcgcac agcgactggc
     4381 aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc
     4441 taaacgccac ggcgtgaaca tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct
     4501 gcaaccaggc gataagctga cgttgtttgt gaaaaacaac aacatgccag attcctgaca
     4561 aaccagataa taaaaaggca ccgattcccc cggtgccttt tttatttatg ctgctttatg
     4621 tgcttctacc atgatgatat cactggtgaa agagccatca ttctgcaagg caaaatacgt
     4681 tctcacctct gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg
     4741 cgtacgcatt ctcgcgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt
     4801 atcaactatc agattggctt cattgattaa cctcaaccac tcaccgctgg cgtagtttcg
     4861 tacgtgagag gtatcgcgta atgcttctac cgtctgtaac cagatgtcgc gcactggatg
     4921 acccggagac attacgtcca tcacaatcat cctaccgcca ggtttcaata tcctattcac
     4981 ttctcgcagt gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat
     5041 atcaaatgcg ttatcggcaa atggcagact ttcggcataa ccctggcggg tggtgatatt
     5101 tttcagttgc cgggcttcgg cagcttgtgc cacgacatcc agcatttggg cagataagtc
     5161 atacgccacc accgcgctca cgttctgcgc agcgacaaag ctggcatgcc ctgctccgca
     5221 gcccatatca agtacacttg tatcaggata atcagccaga cgcaccgcca ggcgctgtaa
     5281 atctcgcccg gatgcatgta cggtactggt taaatactca ctggcctgcg agctaaactg
     5341 cttttctacg tggtcatggt gggattgtgt tgtcattgcg ctgtcctttt gttatttgag
     5401 tataaaaggg cagcgccaaa ctttggtctg ccctgtggca gacctgacat accttattta
     5461 tcaggtttgc cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc
     5521 agtacagaag cttcgctgac gttcagacca cggtagaaaa caaaatcgag cggacggcca
     5581 aacgcccgac ggcgctgatc gtcggtaaaa cgcacctggc gcagcgacat ttcccgcgca
     5641 aagcgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
     5701 accgggccgc tgtgatgagc tatctgatcg ccaataggaa gtaactgctt actataaaca
     5761 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtatca
     5821 ggtaatggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
     5881 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
     5941 tgtggcagta cgaaagcggg tacctgatcg gcggcaagat agttagcggt cgcaaactgt
     6001 actaactctg gcgttgtctg cgcttcctgc aataacacca gatgtgcatc tttgccgtag
     6061 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
     6121 cgaatacgct cttcggtact taacggttcc ccaggtggta atgcctggcc gatgctcgca
     6181 aaagaccccg gcggtaagat cctttccgca ggttgtccgg caacatagcg catggcatag
     6241 gtgttttttc gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga
     6301 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
     6361 gtaagcaagt gcttacaccg gtcagaattt tgctcattgt tgagaacatt aaatcggatg
     6421 acatatcacg catcctgatg gtttaatgac aaataaattc aaaatatctc ttctattttg
     6481 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg
     6541 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
     6601 gggcagtgaa aaagctggag atgaaacttg gcgttagcct gcttaatcgg accacgcgac
     6661 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
     6721 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttgc
     6781 gcatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttgatt aagcctttcc
     6841 gtgaacgcta tccggaagtc actttgtcgc tagtctcctc cgaaacgatt attaatttga
     6901 tcgaaagaaa agttgatgtc gcgatacgcg ccggtacgtt aacggattcc agcttacgtg
     6961 ccaggccgtt atttaatagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
     7021 acgggaagcc agaaacgatc gacgatttaa agcaacatgt ttgcctggga ttcactgaac
     7081 ccgcttccct caatacctgg ccgatagcct gtagtgatgg acaattacat gaggtgaagt
     7141 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
     7201 ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt
     7261 taatggcaga taaagtgttg ccagtggcaa tgccattcag tgcagtctat tacagcgacc
     7321 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
     7381 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct ttcgggctaa
     7441 ccaggcggtc gttgcaatcc agggcggcga tcgcttttta tctttggcat cgagctgtaa
     7501 attttgtgcc ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga
     7561 gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc
     7621 tgcgatacga gcaataacct catctttcag ggccttacca tacgccagcg tcatatagga
     7681 agtaatatgg atgccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg
     7741 agagagttca atctggttag tagcgatgtt ttcagcgcca acagcagcaa tcgccttttc
     7801 catcaatggg atcgtgaagt tggaaatacc gatctcacgc gtcagccctt gttttttggc
     7861 ttccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg
     7921 gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt tcagacttgg
     7981 gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg
     8041 acgtggcacg ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg
     8101 tgcggtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac
     8161 gtcgtctttc agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt
     8221 ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgag gaaaaagagc
     8281 gtaaaatgca gaggattttt gcgattctgg caataataga tatacagacg aaaaaaaacc
     8341 tcgagcgtta actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga
     8401 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt
     8461 acaaccaccg gttttatgac cggctt
//
LOCUS       DAPPNJ010000183         8454 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000183 DAPPNJ010000000
VERSION     DAPPNJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.185"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(145..4032)
                     /gene="purL"
                     /locus_tag="RPM73_003634"
                     /gene_synonym="purI"
     CDS             complement(145..4032)
                     /gene="purL"
                     /locus_tag="RPM73_003634"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDX6521947.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRMGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4290..5846
                     /gene="mltF"
                     /locus_tag="RPM73_003635"
     CDS             4290..5846
                     /gene="mltF"
                     /locus_tag="RPM73_003635"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDX6521948.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5843..6379)
                     /gene="tadA"
                     /locus_tag="RPM73_003636"
     CDS             complement(5843..6379)
                     /gene="tadA"
                     /locus_tag="RPM73_003636"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDX6521949.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6404..7039)
                     /gene="yfhb"
                     /locus_tag="RPM73_003637"
     CDS             complement(6404..7039)
                     /gene="yfhb"
                     /locus_tag="RPM73_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDX6521950.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7248..8096
                     /locus_tag="RPM73_003638"
     CDS             7248..8096
                     /locus_tag="RPM73_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDX6521951.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            8152..8412
                     /gene="yfhL"
                     /locus_tag="RPM73_003639"
     CDS             8152..8412
                     /gene="yfhL"
                     /locus_tag="RPM73_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDX6521952.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         2048 a         2319 c         2176 g         1911 t
ORIGIN      
        1 aagcgacacg gtaaaccacc cattgtcgtg atttacagac acaaaaaagc tcccagagtt
       61 gggagcttat gatagcggtt gtatttaatg ccggatgcgg cgtgaatgcc tcatccgggc
      121 tgcaaaacca atgagtttga cggcttaccc caactgctta cgcgcattgc ggaaaatgcg
      181 catccatggg ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa
      241 aacacgttcc gggtgcggca tcataatggt gactcgacca ctttcagtcg tgaccgccgt
      301 aataccgttc ggagaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt
      361 atcgacatag cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc
      421 acgcacttcc acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat
      481 cccctgcaac agcagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa
      541 gcgatcggag gtattgcgca caaaacgtgg ccacaactca ctacctggga tcagttcgcg
      601 caggttagac atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacggtg
      661 gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac aggattgact tcgcccaacc
      721 ttcaccggca cccagcacat caccgtagga gaaaccaccg cacgcgacca gggcgtggaa
      781 atcttccagg cccatgcgtc cagtcagcag gtcactcata tgcacatcga ttgcatcgaa
      841 gcctgcacgg tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag
      901 cacagcaact ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat
      961 atcgaacgac agtttcacat tcaggcccgg atcggcgtcg tttgatttcg cctggtgctc
     1021 ctggtcagca cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca
     1081 ccagacacgc aacgtggtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac
     1141 aaaacggtca ccggaaaccg cctgccctac ataatggaca caatcagcaa gcccatgctg
     1201 tgccagtacg gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag
     1261 ttcttcgtta aacaacgccg ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat
     1321 gccgcaatga ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg
     1381 gtcgtggtat gcgagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc
     1441 tttcagttgc gcgacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg
     1501 cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc
     1561 gttatcttcg gtagaaagct gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa
     1621 tgcagaaatc accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca
     1681 gcgggttttc atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca
     1741 aagctcttcg cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc
     1801 tgccgccgcc atccagttgg cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc
     1861 ggcaatattg gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag
     1921 cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc
     1981 ggtagtgacc gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc
     2041 taccatgcca gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt
     2101 cggcagatgc agtacgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc
     2161 atcgcccttc gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag
     2221 gacgtccagc ggcagatcga tcggctgatt atcaaaatga cgatcgtgca gagaaagatg
     2281 cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc
     2341 gtcaaacagc ggtaattgat cggcagcaac cgccagcacg tagcgttcct gggattcgtt
     2401 acaccagatt tccagcgggc tcatgcccgg ttcgtcgttc agaatatcgc gcagttcgaa
     2461 tttaccgccg cgtccgccgt cgctcaccag ttccggcatc gcgttagaaa gaccgcctgc
     2521 gccaacgtcg tggataaaca gtattgggtt ggcatcacca agctgccagc aacggtcaat
     2581 cacttcctgg cagcgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag
     2641 gtcggcatca gactgaccag acgccatgga agacgccgct ccgccgccaa gaccgatgtt
     2701 cattgccggg ccgccgagaa cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac
     2761 gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg
     2821 cagctcttcg ccgttgtggc tgttcacttt ttcttcataa gtacggaagt agccattcag
     2881 tgccggacga ccaaattcgt tgttaaacgc cgcgccgccc agcgggcctt cggtcatgat
     2941 gtccagcgcg gtgacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcgaa
     3001 gccaggaatt cgcaggttgg aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc
     3061 gcgcccggtg gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca
     3121 cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc atcagaatat gcgccggttc
     3181 ctgatggaaa tcgtagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc
     3241 ttccattacg gcggcgttat ctttataagc agagagaacg taatctggcg tggtttcgaa
     3301 ggtgtttttg atcatcttaa acagcgattt cggctgttgc tcaccgtcga taacccaatc
     3361 ggcgttaaaa atcttgtggc ggcagtgctc ggagttcgcc tgagcgaaca tatacagctc
     3421 gatgtcgttc gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc
     3481 ttccgccaga gccaggccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc
     3541 cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc
     3601 atctaaatca aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg taacctgctg
     3661 ccattgttca ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg
     3721 gtttacctgt tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga
     3781 gatggtgcca ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag
     3841 tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc
     3901 attgaggtca gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc
     3961 ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag
     4021 aatttccatc ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg
     4081 gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca
     4141 tcgtgaatta gcaacgcgtg ccgccaatgg atgtaataag ttgccatctg gcgcaggttt
     4201 acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg
     4261 ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca
     4321 tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta
     4381 aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc
     4441 atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg
     4501 cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca
     4561 gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca ggacttgtct
     4621 ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac
     4681 aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg acggcggagc
     4741 aactcaccgt tgcaccgggt catgtggtgg ttaacgatct ccagaccctg aaagaaacaa
     4801 aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag
     4861 atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc
     4921 agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt
     4981 ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg
     5041 aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg
     5101 attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt
     5161 taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg
     5221 cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga
     5281 tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac
     5341 agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg
     5401 tgccggagaa cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata
     5461 tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg
     5521 taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct
     5581 acgctcgtgg acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat cagattagcc
     5641 tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc
     5701 aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt
     5761 ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga
     5821 aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa
     5881 tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta
     5941 ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag
     6001 atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt
     6061 ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga
     6121 tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg
     6181 cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc cgattgttat
     6241 gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca
     6301 gcgcgtgacg catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg
     6361 ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga
     6421 gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct
     6481 gattactgtc gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt
     6541 gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct
     6601 gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt
     6661 caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt
     6721 aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca
     6781 cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca
     6841 gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca atggctataa
     6901 tcggtaacaa cggcaggaca agtaacgcat tcaacggttg gcgacgcagt aaatagcgca
     6961 gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac
     7021 gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc
     7081 tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc
     7141 taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg
     7201 tctctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac
     7261 ttcgtatccg tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc
     7321 tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg
     7381 gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg
     7441 cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca
     7501 ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag
     7561 aaaatactgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat
     7621 gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc
     7681 tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc
     7741 gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag
     7801 ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag
     7861 tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt
     7921 ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt
     7981 gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg
     8041 aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa
     8101 agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg
     8161 ttaatcacta aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg
     8221 atttcaatgg gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg
     8281 cactacgaga caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat
     8341 ccggcgcatg tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg
     8401 gataaaattt aatattctac tctggaagta gagtattaat tatattactg ggaa
//
LOCUS       DAPPNJ010000184         8410 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000184 DAPPNJ010000000
VERSION     DAPPNJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.186"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(60..1652)
                     /locus_tag="RPM73_003640"
     CDS             complement(60..1652)
                     /locus_tag="RPM73_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDX6521953.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="RPM73_003641"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="RPM73_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX6521954.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="RPM73_003642"
     CDS             complement(2850..3968)
                     /locus_tag="RPM73_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDX6521955.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="RPM73_003643"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="RPM73_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDX6521956.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="RPM73_003644"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="RPM73_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDX6521957.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="RPM73_003645"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="RPM73_003645"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDX6521958.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="RPM73_003646"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="RPM73_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDX6521959.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="RPM73_003647"
     CDS             complement(7218..7283)
                     /locus_tag="RPM73_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521960.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="RPM73_003648"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="RPM73_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDX6521961.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2068 a         2233 c         2018 g         2091 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat cgtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct
     1321 tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca
     1501 aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggtcaaa tcggcgaata
     5941 cgataccgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta
//
LOCUS       DAPPNJ010000185         8407 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000185 DAPPNJ010000000
VERSION     DAPPNJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.187"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            352..1692
                     /gene="dcuB"
                     /locus_tag="RPM73_003649"
     CDS             352..1692
                     /gene="dcuB"
                     /locus_tag="RPM73_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDX6521962.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1770..3416
                     /gene="fumB"
                     /locus_tag="RPM73_003650"
     CDS             1770..3416
                     /gene="fumB"
                     /locus_tag="RPM73_003650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDX6521963.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3539..4168
                     /locus_tag="RPM73_003651"
     CDS             3539..4168
                     /locus_tag="RPM73_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDX6521964.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4307..5728)
                     /gene="melB"
                     /locus_tag="RPM73_003652"
     CDS             complement(4307..5728)
                     /gene="melB"
                     /locus_tag="RPM73_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDX6521965.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5831..7186)
                     /gene="melA"
                     /locus_tag="RPM73_003653"
     CDS             complement(5831..7186)
                     /gene="melA"
                     /locus_tag="RPM73_003653"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDX6521966.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7469..8377
                     /gene="melR"
                     /locus_tag="RPM73_003654"
     CDS             7469..8377
                     /gene="melR"
                     /locus_tag="RPM73_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDX6521967.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2161 a         2220 c         2058 g         1968 t
ORIGIN      
        1 acccacaaat gggtagatca gattaatctt taaacctaat gatatctgcc ctgagaacaa
       61 aaaatagacc gataaatatc aataagataa cagcaaacaa aacattaaca tctgcgcagt
      121 acaaactata aacccatcgc cagagagttt ttctctctga aaaagccgct tatcacagtg
      181 cataaatttg ccgctgcttt aatcagccaa tatttactgt gaggtatttg ctaaagccgg
      241 taacgaccaa acggatattt agtcaggctc tgaaaacagt tcatacaaaa cagaacgtga
      301 ctgtgatcta ttcagcaaaa atttaaatag gattatcgcg agggttcaca catgttattt
      361 actatccaac ttatcataat actgatatgt ctgttttatg gtgccagaaa gggtggtatc
      421 gcgctgggtt tattaggcgg tatcggtctg gtcattctgg tcttcgtctt ccaccttcag
      481 ccaggtaaac caccggttga tgtcatgctg gttatcattg cggtggtggc ggcatcggcg
      541 accttgcaag cttcgggcgg tcttgatgtc atgctgcaaa ttgccgagaa gctgctgcgc
      601 cgcaacccga aatatgtctc aattgtcgcg ccgtttgtga cctgtacact gaccattctt
      661 tgcggtacgg gtcatgtggt ttacaccatt ctgccgatca tctacgacgt cgccattaag
      721 aacaacatcc gtccggaacg tccgatggcg gcaagttcta tcggtgcaca gatggggatt
      781 atcgccagtc cggtgtcggt tgcggtcgtg tctctggttg caatgctggg taatgtcacc
      841 tttgatggtc gccatcttga gttcctcgac ctgctggcaa tcaccattcc atcgacgtta
      901 atcggtatcc tggcgatcgg tatcttcagc tggttccgcg gtaaagatct ggataaagac
      961 gaagagttcc agaaattcat ctccgtaccg gaaaaccgtg agtatgttta cggtgatacc
     1021 gcgacgctgc tcgataaaaa actgccgaaa agcaactggc tggcaatgtg gattttcctc
     1081 ggtgcaatcg ctgtagtcgc ccttcttggt gctgattcgg acctgcgtcc atccttcggc
     1141 ggcaaaccgc tgtcgatggt actggttatt cagatgttta tgctgctgac cggggcgctg
     1201 attattatcc tgaccaaaac caatcccgcg tctatctcaa aaaacgaagt cttccgttcc
     1261 ggtatgatcg ccatcgtggc ggtgtacggt atcgcatgga tggcagaaac catgttcggt
     1321 gcgcatatgt ctgaaattca gggcgtactg ggtgaaatgg tgaaagagta tccgtgggcc
     1381 tatgccattg ttctgctgct ggtttccaag tttgtaaact ctcaggctgc ggcgctggcg
     1441 gcgattgttc cggtcgcgct ggcgatcggc gttgatccgg catacatcgt ggcttcagca
     1501 ccggcttgct acggttatta catcctgccg acttatccga gcgatctggc agcgattcag
     1561 tttgaccgtt ccggcaccac ccacatcggt cgcttcgtca tcaaccacag ctttattctg
     1621 ccggggttga ttggtgtgag cgtatcgtgc gtcttcggct ggatcttcgc cgcgatgtac
     1681 gggttcttat aaatgcactt tgcgtgccgc ccgtgactac gcggcacgcc attttcgaat
     1741 aacaaataca gagttacagg ctggaagcta tgtcaaacaa accctttatc taccaggcac
     1801 ctttcccgat ggggaaagac aataccgaat actatctact cacttccgat tacgttagcg
     1861 ttgccgactt cgacggcgaa accatcctga aagtggaacc agaagccctg accctgctgg
     1921 cgcagcaagc ctttcacgac gcttctttta tgctccgccc ggcacaccag aaacaggttg
     1981 cggctattct tcacgatcca gaagccagcg aaaacgacaa gtacgtggcg ctgcaattct
     2041 taagaaactc cgaaatcgcc gccaaaggcg tgctgccgac ctgccaggat accggcaccg
     2101 cgatcatcgt cggtaaaaaa ggccagcgcg tgtggaccgg cggcggtgat gaagaagcgc
     2161 tgtcgaaagg cgtctataac acctatatcg aagataacct gcgctattca cagaatgcgg
     2221 cgctggacat gtacaaagag gtcaacaccg gcactaacct gcctgcgcaa atcgacctgt
     2281 acgcggtaga tggcgatgag tacaaattcc tttgcgttgc gaaaggcggc ggctctgcca
     2341 acaaaacgta tctctaccag gaaaccaaag ccctgctgac tcccggcaaa ctgaaaaact
     2401 tcctcgtcga gaaaatgcgt accctcggta ctgcagcctg cccgccgtac catatcgcgt
     2461 ttgtgattgg cggtacgtct gcggaaacca acctgaaaac cgtcaagtta gcaagcgctc
     2521 actattacga tgaactgccg acggaaggga acgaacatgg tcaggcgttc cgcgatgtcc
     2581 agctggaaca ggaactgctg gaagaggccc agaaactcgg tcttggcgcg cagtttggcg
     2641 gtaaatactt cgcgcacgac attcgcgtta tccgtctgcc acgtcacggc gcatcctgcc
     2701 cggtcggcat gggcgtctcc tgctccgctg accgtaacat taaagcgaaa atcaaccgcg
     2761 aaggtatctg gatcgaaaaa ctggaacaca acccaggcca gtacattcca caagaactgc
     2821 gccaggccgg tgaaggcgaa gcggtgaaag ttgaccttaa ccgcccgatg aaagagatcc
     2881 tcgcccagct ttcgcaatac ccggtatcca ctcgtttgtc gctcaccggc accattatcg
     2941 tgggccgaga tattgcacac gccaagctga aagagctgat tgacgccggt aaagaacttc
     3001 cgcagtacat caaagatcac ccgatctact acgcgggtcc ggcgaaaacc cctgccggtt
     3061 atccatcagg ttcacttggc ccaaccaccg caggccgtat ggactcctac gtggatctgc
     3121 tgcaatccca cggcggcagc atgatcatgc tggcgaaagg taaccgcagt cagcaggtta
     3181 ccgacgcgtg tcataaacac ggcggcttct acctcggtag catcggcggt ccggcggcgg
     3241 tactggcgca gcagagcatc aagcatctgg aatgcgtcgc ttatccggag ctgggtatgg
     3301 aagctatctg gaaaatcgaa gtagaagatt tcccggcgtt tatcctggtc gatgacaaag
     3361 gtaacgactt cttccagcaa atcgtcaaca aacagtgcgc gaactgcact aagtaacctc
     3421 ttcggcccag cgcctggcag catgctgcca ggtgatcccc ctgggccacc tcttttgcga
     3481 ttgtaatttc acgcttgctg gtgaatagtc agtattttcc ctgatttgcg aactcaccat
     3541 gacccgcaca ctcaagccgt taattcttaa caccggcgca ctggcgctaa cgttaatcct
     3601 gatttatacc ggcatttcgg cccatgacaa actcacctgg ctgatggaag tgacaccggt
     3661 gattattgtc gtgccgctac tgcttgccac cgctaaacgt tatccgttaa cgccgctgct
     3721 ctatacgctc attttctttc acgccatcat cctgatggtc ggcgggcaat acacctacgc
     3781 gaaagtcccc gttggttttg aggtgcagga atggctgggg ttgagccgta atccgtatga
     3841 caagctgggg cattttttcc aggggctggt gcctgcactg gtggcacgag aaattctcgt
     3901 gcgcgggatg tacgtgcgcg gacgtaaaat ggtggcgttc ctggtctgct gcgtggcgct
     3961 ggcgataagc gcgatgtatg aattaatcga gtggtgggcg gcgctggcaa tgggccaggg
     4021 agcggatgat tttctcggca cccagggcga ccagtgggac acgcaatccg atatgttctg
     4081 cgcgctgctt ggcgcattaa cgacggtgat attcctcgct cgctttcatt gccgccagct
     4141 acggcgcttt ggcttgataa ccggataaca acgtgcgctt tgttgatgcc ggatgcggcg
     4201 tgaattcctt atccggtcta caaaatttgc aaatccaaag cattacatta agacgtggcc
     4261 tgataaacat atcgcatcag gccattttcg ttagctattg tccacgtcag gctttcacat
     4321 cactcactgc accgaccgga atatcagcat gaacaggctc gggcggtact ttgcgatatt
     4381 tatccagcag atgaatctgg atcctgcgca gcgtgtcgcc attgaggcga tagaaacgga
     4441 agtagagaat cagcgttacc atgaaaaaca gagttggtag agcaatcata ataaactgca
     4501 tacctaatag ggcttgcgta gactgttcaa cattcggtac atagccaatc atccctaaca
     4561 caaccgcaat gaaaaaagcc gcaaaggctg aaccgccctt caccaccata gtctgcacgg
     4621 agtaagcgat actttcacag cgtacgtgca gtttatattc accgtaatca acggtatctg
     4681 ccaccatgat gacctgtaat acccagaaaa gcgccgttcc cacattcagc aaaatacccg
     4741 caatcacaat gaggacgacg ttgtgatagc tcattaatgc cattaacagg agaacaccac
     4801 agcttaacac cggaagaata gatgctccgg cccataaaat gcgtcgggat aatgatttaa
     4861 ctaagcgtgg gaagaatact aacgtcacca ggttagcagc tcccgcatac gacagataat
     4921 aggggaacaa atccgcatca ccaataacat atgagaaata atagatagca aagccggtaa
     4981 taatattgct ggctacatta taagcaagag ccatacccaa gaggcatgaa agctgatcgt
     5041 ttttataaat aagcgcaacg atggctttaa gtgtcagatg gcttccttca gcagacggtt
     5101 gattgtctga cgaaaagact tcatgcacat tgcgcagagt gatgatggtt gaaacaataa
     5161 aaaaggcgat cagtaccaga gtgaacatct gaaagccaaa tccccgatcg ccaccgccga
     5221 cataattaac aaatggtagc gtcacacctg ccgtaacaaa gcccgccaga ctggcaaaaa
     5281 aacgcggata aggaaccagt tgttcacgct cacgtttatc gagcgtgatg gttggaacca
     5341 gcgaccagaa gggaatatcc ataatggtgt aagtcatgcc ccagaggatg taggtcacgc
     5401 aaacaaagac aatctgagta gtaccttcaa acagatgcgc actaaagagg agaaataaga
     5461 ttacagagtt tgccaacgta ccgatcagga tccagggttt gaacttaccc catcgcgatc
     5521 gcgtagcatt tacaatccat cccataatcg gatcgttaat agcatcccag atcctcgcca
     5581 ccagaaacaa agtaccgacc aaacccacag acagcccgac gacatcggtg tagtaataca
     5641 tgaggtacat atacacaatg ccgatcgcaa aatccttccc gaacgctcca aatccataac
     5701 tgagttttgt agtcattgaa atgctcatag ggtatcgggt cgctgttacc aacgcccgcc
     5761 ttctgttatt ggtatcgtca gaggtgtgcg ccggaataaa tcccccggcg cagtagcgtt
     5821 tagtcgcgtt ttaacggtgc aaccagcctg gcagccagtc gccgtgggcg gcaatcaggt
     5881 cgtcaacaag agcatatatt tcgtcaatgc ccagcacggc ggcagtatgc gggtccatca
     5941 tcgcggcgtg gtaaacacgg tcgcgatttt ccgtaagaat agcttcggtc agcagcgtct
     6001 gtacgttgat gttggtttgc atcagggcgg ccagatgcga aggtagcgta ccgactttgg
     6061 ttggctgaat gccattagca tcaaccagac aggctacttc cacgcaacat ccttgtggca
     6121 ggttatcaat caaaccatcg ttacggacgt tgccgtaaat cacactcggc tcgccagtcc
     6181 agatagcgtt catgattgtg ctggcatatt cccgtgacgg tttaatatca atccgggagg
     6241 cgtttttata ctcctccagc tctttatgcc agttcgccag ctgctcgacg cagcgtttcg
     6301 ggtactcatc cagcggtact ttataacgct caatcaaatc ctcacgacct ggcttaataa
     6361 accacggcgt atattcggca aaatgttctg acgattccgt gacgaagtag cccagctttt
     6421 tgaacatttc atagcgcaca atattctggc agcgagtatt accatgaata tttggcttcg
     6481 gtgcctgccc tgcttcataa gccgccagca gttccgggta gagattcacg taactgccgt
     6541 cggcggtttt gcgctccagc tccaggtaaa acgccatatg gttgatacct gcgcagcggt
     6601 aacgcagcgt agctgggtcg atattgagat cacgcgccag ctcttccgcc gttccctgca
     6661 ccgaatggca cagcccgacc tgtttgatat gcggataacg ggcatacatc gcccaggtat
     6721 tcatcgccat ggggttaaca tagttgagca tggtggcatc ggggcagact tccgtcatgt
     6781 cctcgcaaat ttgccacaga tgcggaatgg tacgtagcgc gcgcataata ccgcccggcc
     6841 ccaacgtatc ggcaatggtt tgttccagac catgccgctt acagacctcg aaatcagtca
     6901 ccgtgcaagg ttcataaccg ccaatctgaa atgccaccac gacaaaatcg gcatcctcta
     6961 aggcttcttt ctgttgggtg tggcaggtga ttttgccgct ggcccctgct gaatccatca
     7021 gcttacgcac cacaatatgc gactcttcca ggcgggtggg atcaatgtcc atcagggcaa
     7081 tatgcgccgt tttcagcgcc tcgcgatgga acacatcacc aagaatattt ttaacgaaaa
     7141 tcgtcgaacc agcgccgata aatgtaattt tgggtgcagt catcatgcag atctcctggc
     7201 ttgcttgaat aacttcatca tggcaggcga atctgcagaa aacctccggc ttccaggttg
     7261 agcattccca taaactcaga tcctgcgtga agcagcagta aatctgagtt tatggtaata
     7321 agttacctga aaagcagagt tttcggcctc atgatgctcg gacgctcccc tcgcctctct
     7381 gggaagaggg tgaaaaccgt gctcccactc gcagtcatcc tccctcactc ctgccataat
     7441 tctgatattc caggaaagag agccatccat gaatacagat acgtttatgt gcagcagcga
     7501 cgaaaaacag acccgcagcc cgctgtcgct gtattcggaa taccagcgga tggagattga
     7561 gtttcgcgca ccgcatatca tgcccaccag ccactggcat ggtcaggtcg aagtgaatgt
     7621 gcctttcgat ggcgatgtgg aatacctgat caacaatgaa aaagtgaata tcaatcaggg
     7681 tcatatcacg ctgttctggg cctgtacacc gcaccaacta acagataccg gaacctgtca
     7741 gagcatggcg atttttaatc tgccgatgca tctgtttctc tcctggccgc tggataaaga
     7801 cctgattaac cacgtcactc acggcatggt gatcaaatca ctggccacac agcaacttag
     7861 cccatttgaa gtgcgccgct ggcagcagga attaaacagt ccgaacgagc aaattcgcca
     7921 gctcgccatt gatgaaattg gcctgatgct caagcgattt agcctctctg gctgggaacc
     7981 gattctggtc aataaaacct cgcgcacaca caaaaacagc gtctcgcgcc atgcgcaatt
     8041 ttatgttagc cagatgctgg gctttattgc cgaaaactat gatcaggcgc tgaccatcaa
     8101 cgatgtggct gagcacgtca aacttaacgc caactatgca atggggatat tccagcgggt
     8161 catgcaattg acgatgaaac agtacattac cgcgatgcgc atcaaccacg ttcgcgcgtt
     8221 actgagcgat accgataaaa gtattctcga tattgccctg acggcaggct ttcgttcaag
     8281 tagtcgtttt tacagcacgt tcggcaaata tgtcggcatg tcgccgcaac aataccgcaa
     8341 acttagccaa cagcgccgcc agacgtttcc cggctaaaga cgatatcagt atcagccaaa
     8401 aaaatag
//
LOCUS       DAPPNJ010000186         8373 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000186 DAPPNJ010000000
VERSION     DAPPNJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.188"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(201..1091)
                     /gene="malG"
                     /locus_tag="RPM73_003655"
     CDS             complement(201..1091)
                     /gene="malG"
                     /locus_tag="RPM73_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDX6521968.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1106..2650)
                     /gene="malF"
                     /locus_tag="RPM73_003656"
     CDS             complement(1106..2650)
                     /gene="malF"
                     /locus_tag="RPM73_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDX6521969.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2804..3994)
                     /gene="malE"
                     /locus_tag="RPM73_003657"
     CDS             complement(2804..3994)
                     /gene="malE"
                     /locus_tag="RPM73_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDX6521970.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4359..5474
                     /gene="malK"
                     /locus_tag="RPM73_003658"
     CDS             4359..5474
                     /gene="malK"
                     /locus_tag="RPM73_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDX6521971.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5546..6886
                     /gene="lamB"
                     /locus_tag="RPM73_003659"
     CDS             5546..6886
                     /gene="lamB"
                     /locus_tag="RPM73_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDX6521972.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7038..7958
                     /gene="malM"
                     /locus_tag="RPM73_003660"
     CDS             7038..7958
                     /gene="malM"
                     /locus_tag="RPM73_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDX6521973.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8187..>8373
                     /locus_tag="RPM73_003661"
     CDS             8187..>8373
                     /locus_tag="RPM73_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6521974.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLNRDN
                     FIYISNIFSNIKQRNEKI"
BASE COUNT         2086 a         2160 c         2158 g         1969 t
ORIGIN      
        1 gagcacgaaa gagaattatc gtaagtggga aaacaaataa cgtaaaaata ataagttcta
       61 tgatgggaat attaaccggc gaacgattca gattgcagac gaaagaaaaa aaggcgctcc
      121 gtggagcgcc gaataatagt cacaagttgg gataacgtaa gttgagggtg cagcggcata
      181 acattggcag aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc
      241 cagcgttgag ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg
      301 gcaaagtcac cccacaggta gttttgcggg ttgaggtatt gctgcatccc cacggccagg
      361 gtgtagctat ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt aatggcagcg
      421 ataaacgaca ggatgaacac caccgccaga atcggtactg acagcggcaa caggacaagg
      481 cggaaggcct gccacggtgt cgcaccatcc agcgccgccg cttcttccag cgaactgtcg
      541 atggtttcga aatagccttt gatggtccag acatgcagcg caatcccacc cagatacgcg
      601 aaaattacgc cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca
      661 aacaacgcat acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt
      721 cctttcagca gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg
      781 gtggagagcg ccacaatgcc aatcgcggaa atcccggcga cctttaccga gttccacagc
      841 cacagcagta ccgggaatgg cggtggcgta atgcgaccat cagcctgttc aacgctaaaa
      901 cctaacgcca gtttccagtg atcccaggag atttgctccg ggatcaggct accggtcgcg
      961 aagtttccct gacgcagcga gatagcgacg accatcagca gcgggaacat aatcgccgcg
     1021 ataaaaagta gcagtagcag gtgagtaata aacaaacgtg ctttttgcga tttcggttgg
     1081 accattgcca tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca
     1141 ctatcgccag cgcacccacc agcaggaaga tcagcgtggc aattgctgca gccagaccga
     1201 agtcctgacc cccgccgcct tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg
     1261 tataaccggc tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagctgaa
     1321 tcagcacgaa gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt
     1381 taatcagcag cggcagcgta atcttaaaga agttctggaa cggacctgcg ccatccattg
     1441 ctgaggcttc atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca
     1501 tgtacggata acccagccag gtattgacga taatcagcat cgtgcgggcg gtggtcggat
     1561 cgctgaacca ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atttcaccga
     1621 agctctggtt aaacaatcct ttgaaaatca agattgaaat gaacgatggc accgcgtagg
     1681 gcagaatcag caggacgcga tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac
     1741 atgccagaac catgccgacc gccaccgtta aaaagacagt gatcagcgag aacaccacgg
     1801 tccagacgaa aatggcgagg aacggtttct gaatgccttc gtcggtaaag acgcgggtaa
     1861 agtttttcca gccggtggtc acggtgtaac cggggcttag cttttcatca ccccagttgc
     1921 cgtcggcggt aatggactgg taaaagccaa tttggttatt cggacgatat ttcacgccgc
     1981 tctgattatt cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa
     2041 actggcgcag ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac
     2101 tcagcgcctg acgattctgg gtaatcacgc gtagattcgc gcgttcgcct tcgggctggg
     2161 cggtcgtttc tttcagttgc agtttttgct cgccgccaaa tttaaaagcg tcggaaaggt
     2221 aatttttgcc ggtttcgccg tcactgagcg ccagttgcca ctcatcgccc gccgggtaaa
     2281 gaccaaagtt ataggttttg cctgcttgcc aggagcgatc taacaacact tcctgtgcac
     2341 gttcaaaagt cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga
     2401 ccagagggaa gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg
     2461 cgtaggcttt acgattggcg aaaatataca gccccgctga actcaatatc agcgtggtaa
     2521 tggcgaacag gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc
     2581 cgagcagacc tagcactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa
     2641 tgacatccat ggggttcttc ctcattccag gacggataag gctttcacgc cttatccgac
     2701 aacaactgcc tgatgcgaca ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt
     2761 aggccggaca aggcgttcac gccgcatccg gcatttcaca gcattacttg gtgatacgag
     2821 tctgcgcgtc tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg
     2881 cagtacgcac ggcataccag aaagcggaca tctgcgggat gttcggcatg atttcacctt
     2941 tctgggcgtt ttccatggtg gcggcaatac gtggatcttt cgccaactct tcctcgtaag
     3001 acttcagcgc cactgcaccc agcggtttgt ctttattgac cgcttccaga ccttcgtcag
     3061 tcagcagata gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa
     3121 taccggcgct cagcacgcca acgaacggtt tagatggttg acccttgaag gtcggcagta
     3181 ccgttacacc ataattcact ttgctggtgt cgatgttgga ccatgcccac gggccgttga
     3241 tggtcatcgc tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg
     3301 cattcatgtg tttgttttta ataaggtcaa ccaggaaggt cagacccgct ttcgcgccag
     3361 cgttatccac gcccacgtct ttaatatcgt acttgccgtt ttcatacttg aacgcataac
     3421 ccccgtcagc agcaatcagc ggccaggtga agtacggttc ttgcaggttg aacatcagcg
     3481 cgctcttacc tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg
     3541 gtgggttcgg cagcagatct ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt
     3601 aagcaatcag cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct
     3661 ggaacgcttt gtccggggtg atttcagcca acaggccaga ttgagcgtag ccaccaaagc
     3721 ggtcgtgtgc ccagaagata atgtcagggc catcgccagt tgccgcaacc tgagggaatt
     3781 tctcttccag tttatccgga tgctcaacgg tgactttaat tccggtatct ttctcgaatt
     3841 tcttaccgac ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt
     3901 taccttcttc gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata
     3961 atgcgaggat gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg
     4021 ctcgtgaaaa cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc
     4081 ttgcccctac gccccaccgt tgctttgtgt gatctctgtt acagaattgg cggtaatgtg
     4141 gagatgcgca cataaaatcg ccacgatttt tgcaagcaac atcacgaaat tccttacatg
     4201 acctcggttt agttcacaga agccgtgttc tcatcctccc gcctcctccc ccataaaaaa
     4261 gccagggggt ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg
     4321 ttgctgtcga tgacaggttg ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa
     4381 atgtaacgaa agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg
     4441 aaggtgaatt cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca
     4501 tgattgccgg gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga
     4561 atgacactcc gccagcagaa cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc
     4621 cccacctgtc agtagcagaa aatatgtcat ttggcctgaa actggcaggc gcaaaaaaag
     4681 aggtgattaa ccaacgcgtt aaccaggtgg cggaagtgct gcaactggcg catttgctgg
     4741 atcgcaaacc gaaagcgctc tctggcggtc agcgtcagcg tgtggcgatt ggccgtacgc
     4801 tggtggccga gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac
     4861 tgcgtgtgca aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga
     4921 tttacgtcac ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg
     4981 acgccggtcg cgtggcgcag gttgggaaac cgctggagct gtaccactat ccggcagacc
     5041 gttttgtcgc cggatttatc ggttcgccaa agatgaactt cctgccggta aaagtgaccg
     5101 ccaccgcaat cgatcaagtg caggtggagc tgccgatgcc aaatcgtcag caagtctggc
     5161 tgccagttga aagccgtgat gtccaggttg gagccaatat gtcgctgggt attcgcccgg
     5221 aacatctact gccgagtgat atcgctgacg tcatccttga gggtgaagtt caggtcgtcg
     5281 agcaactcgg caacgaaacc caaatccata tccagatccc ttccattcgt caaaacctgg
     5341 tgtaccgcca gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc
     5401 cgccagagcg ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg
     5461 agccgggcgt ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa
     5521 gaaaagcaat gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt
     5581 tgccgtcgca gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc
     5641 acgttccggt attggctgga caggtagcgg cggtgaacaa cagtgtttcc agactaccgg
     5701 tgctcaaagt aaataccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg
     5761 tcaggaagtg tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc
     5821 cgtcgcgcaa cagaatgact gggaagctac cgatccggcc ttccgtgaag caaacgtgca
     5881 gggtaaaaac ctgatcgaat ggctgccagg ttccaccatc tgggcaggta agcgcttcta
     5941 ccaacgtcat gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc
     6001 cggtctggaa aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgctcctc
     6061 tgaagctggt ggttcttcct cttttgccag caacaatatt tatgactata ccaacgaaac
     6121 cgcgaacgac gttttcgatg tgcgtttagc gcagatggaa atcaacccgg gcggcacatt
     6181 agaactgggt gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg
     6241 cgcatcgaaa gacggctggt tattcactgc tgaacatact cagagtgtcc tgaagggctt
     6301 taacaagttt gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc
     6361 gcagggttct ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca acaacaacgg
     6421 tcacatgctg cgtatcctcg accacggtgc gatctccatg ggtgacaact gggacatgat
     6481 gtacgtgggt atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac
     6541 cgttggtatt cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg
     6601 ctacgacaac gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct
     6661 cgcacaacaa tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc
     6721 aacctacgcc aagtgggatg agaaatgggg ttatgactac aacggcgata gcaaggttaa
     6781 cccgaactac ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga
     6841 cagcgacgag tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg
     6901 ccggataagg cgtttacgcc gcatccggca taaaaacagg ttgtcattat ctgaaagggg
     6961 cgaaagcccc tctgattatc gggtttagcg cgctattgcc tggctaccgc tgagctccag
     7021 attttgaggt gaaaacaatg aaaatgaata aaagtctcat cgccctctgt ttatcagcag
     7081 ggttactggc aagcgcgcct ggaatcagcc ttgccgatgt taactacgta ccgcaaaaca
     7141 ccagcgacgc gccagccatt ccatctgccg cgctgcaaca actcacctgg acaccggtcg
     7201 atcaatctaa aacccagacc acccaactgg cgaccggcgg ccaacaactg aacgttcccg
     7261 gcatcagtgg tccggttgct gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga
     7321 cgctgaccag cgaagtgaac aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg
     7381 atcagaacat gaccccatca gccttcttcc ccagcagtta tttcacctac caggaaccag
     7441 gcgtgatgag tgcagatcgg ctggaaggcg ttatgcgcct gacaccggcg ttggggcagc
     7501 aaaaacttta tgttctggtc tttaccacgg aaaaagatct ccagcagacg acccaactgc
     7561 tcgacccggc taaagcctat gccaagggcg tcggtaactc gatcccggat atccccgatc
     7621 cggttgctcg tcataccacc gatggcttac tgaaactgaa agtgaaaacg aactccagct
     7681 ccagcgtgtt ggtaggacct ttatttggtt cttccgctcc agctccggtt acggtaggta
     7741 acacggcggc accagctgtg gctgcacccg ctccggcacc ggtgaagaaa agcgagccga
     7801 tgctcaacga cacggaaagt tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg
     7861 atgttgataa ggcgttaaaa ctgcttgatg aagctgaacg cctgggatcg acatctgccc
     7921 gttccacctt tatcagcagt gtaaaaggca aggggtaatt acgccccaca gtgctgattt
     7981 tgcaacaact ggtgcgtctc ctggcgcacc tttttttatg cttccttcct gggatatgag
     8041 cgatttttta tagtaactca cttcttcttc actaagaata tccattatct caatgcctta
     8101 tcagagattc ttttcctttc gccggtagtg tctggacatt caggctactt ttccaggtta
     8161 ttttatttct gttatgcaga ggttttatga taagtcatat cctaaattct ggcggcaata
     8221 actctttgat gaaacatgat gtggtgcaag gaaataatat agtagatctt gatttactac
     8281 gtaatttcaa tggggtgcca ggtttaaaca gagataactt tatttatatc agcaatattt
     8341 tttcaaatat aaaacaacgg aacgaaaaaa tca
//
LOCUS       DAPPNJ010000187         8333 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000187 DAPPNJ010000000
VERSION     DAPPNJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.189"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(77..283)
                     /locus_tag="RPM73_003662"
     CDS             complement(77..283)
                     /locus_tag="RPM73_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDX6521975.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            596..685
                     /gene="kdpF"
                     /locus_tag="RPM73_003663"
     CDS             596..685
                     /gene="kdpF"
                     /locus_tag="RPM73_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDX6521976.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            685..2358
                     /gene="kdpA"
                     /locus_tag="RPM73_003664"
     CDS             685..2358
                     /gene="kdpA"
                     /locus_tag="RPM73_003664"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDX6521977.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2381..4429
                     /gene="kdpB"
                     /locus_tag="RPM73_003665"
     CDS             2381..4429
                     /gene="kdpB"
                     /locus_tag="RPM73_003665"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDX6521978.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4438..5010
                     /gene="kdpC"
                     /locus_tag="RPM73_003666"
     CDS             4438..5010
                     /gene="kdpC"
                     /locus_tag="RPM73_003666"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDX6521979.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5003..7687
                     /gene="kdpD"
                     /locus_tag="RPM73_003667"
     CDS             5003..7687
                     /gene="kdpD"
                     /locus_tag="RPM73_003667"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDX6521980.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7684..>8333
                     /gene="kdpE"
                     /locus_tag="RPM73_003668"
     CDS             7684..>8333
                     /gene="kdpE"
                     /locus_tag="RPM73_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDX6521981.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITET"
BASE COUNT         1725 a         2173 c         2439 g         1996 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcaaaact cagcaatcaa taaaaatcac cagttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      301 gtatctaccc ggtgaatggc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      361 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      421 tacattcatc ctgtaattaa gttacacaaa agttaaatta atactaaaca ttagttaaat
      481 catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt tgcgaaatct
      541 ttgcagccag aattctaccc ttccggtatc acttttaggc cactggaggt gcactgtgag
      601 tgcaggcgtg ataaccggcg tattgctggt gtttttatta ctgggttatc tggtttatgc
      661 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat cgccacgttt
      721 ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
      781 gacattcctc ttcccggtac aacgggcgtt gagcgcgtac tttttcgcgc acttggcgtc
      841 tctgaccgtg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
      901 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
      961 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1021 accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca gatggctggc
     1081 ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccctcatc
     1141 cgtgcgttta cccgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1201 atcacgttat gggtgctcgt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1261 ggtgcgctgc aaaactttca gccttatcag gctgtgaata ccgttgaagg agcgcaacag
     1321 ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1381 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1441 ttcgtgcaga tgctggcgat cttcttgatc ccaacggcgc tgtgctttgc ctttggtgaa
     1501 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1561 atctgcgtag gcgtggtgat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1621 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     1681 agcctgtttg cggtcgtgac tacggcggct tcctgtggcg cggtgattgc gatgcatgat
     1741 tcgtttaccg ctctcggtgg catggtgccg atgtggctga tgcaaattgg tgaagtggtg
     1801 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     1861 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     1921 gagatgaaac tgactgcact ggcgattctg gttaccccga cgctggtgct gatgggcgcg
     1981 gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcaaccctgg cccgcatggt
     2041 tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc
     2101 ggattaagcg ccaactcccc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2161 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2221 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2281 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2341 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2401 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2461 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2521 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2581 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     2641 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     2701 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     2761 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     2821 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     2881 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     2941 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3001 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3061 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3121 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3181 tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagc cggatgctag gcgcgaatgt
     3241 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3301 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3361 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3421 ggaaggccgc agtattgtga tcctcgccaa gcagcgtttt aacctgcgcg agcgcgatgt
     3481 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3541 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgtcgcc atgttgaggc
     3601 taacggtggt cacttcccta ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg
     3661 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     3721 tatcgtcaaa ggcggtatta aagagcgctt cgcgcaactg cgccaaatgg gcattaaaac
     3781 ggtgatgatt accggcgata accgtctgac tgccgccgcc attgctgcgg aagcgggcgt
     3841 cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgattc gtcagtatca
     3901 ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc aacgatgctc cagcgctggc
     3961 gcaggcagat gtcgcggtgg cgatgaactc cggtactcag gcggcgaaag aagcgggcaa
     4021 tatggtcgat ctcgactcta acccgaccaa gttgatcgag gtggtacata ttggcaaaca
     4081 gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg tggcgaaata
     4141 cttcgccatt attccggcgg cgttcgcggc aacgtatccg cagttaaatg cgctgaacat
     4201 catgcgcctt cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4261 tatcgtcttt ttgattcctc tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc
     4321 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4381 tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag gtttaccatg
     4441 cgtggcttac gtccggcatt atcgacattt atctttctgt tattgattac tggcggcgtt
     4501 tacccgctgc tgaccaccgt gctggggcaa tggtggtttc cctggcaggc caacggttcg
     4561 ttgattcgtg aaggtgatac ggtgcggggt tcggcattaa tcgggcagaa ttttaccggc
     4621 aacggctatt ttcagggtcg cccgtcggca acggcagaaa tgccctataa tccacaggct
     4681 tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaacaaat agccgcacgc
     4741 gttgctgcat tacgggccgc taacccggat gccagtacga acgttccggt tgaactggtg
     4801 acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggt ctggcaaatc
     4861 ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc tcacgcaact gatcgcaaaa
     4921 tacagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat tgttgaactc
     4981 aatctggcgc tggataaact tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct
     5041 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg gtgcctgtgc
     5101 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5161 ggatattgtg gttggcgtgg tagaaaccca tgggcggaaa gataccgccg ccatgcttga
     5221 agggctggct gttctgccgc ccaaacgcca ggcgtaccgt gggcggcata tcagcgagtt
     5281 tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5341 cagtaatgcg ccaggttctc gtcatcctaa acgctggcag gatatcgaag aactgctgga
     5401 agctggcatt gatgttttca caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt
     5461 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5521 cgccgacgac gtagtgctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5581 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     5641 caatctgatc gccctgcgtg aactggcact gcgccgtact gccgatcgcg tagatgagca
     5701 aatgcgcgcc tggcgggggc atcctggtga agagaaagtg tggcacacgc gcgacgcgat
     5761 ccttttatgc atcgggcata acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt
     5821 ggcctcgcgc ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg
     5881 cttaccggaa aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc aggagctggg
     5941 cgcggagacg gctacacttt ctgatccagc ggaagaaaaa gcagtagtgc gttatgcccg
     6001 tgaacataat ctcggcaaga ttattctcgg tcgcccggcc tcgcgccgct ggtggcgtcg
     6061 ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc
     6121 gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct cttttaaaga
     6181 caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac
     6241 tttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct
     6301 gcttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttcggtgg ttgctaccgt
     6361 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgctgtctc
     6421 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6481 ccttactgcg ggtgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6541 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6601 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6661 tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgaccccgt gggacgatgc
     6721 catcgcacag tggagttttg ataaaggcct gcctgcgggt gcgggcaccg acacgttacc
     6781 cggtgtaccg taccagattt tgccgttaaa aagcggtgag aaaacctacg ggctggtggt
     6841 ggtggaacca gggaatctgc gtcagttgat gatcccggaa cagcagcgcc tgctggagac
     6901 gtttacgctg ttagtcgcca atgcgtttga gcggcttacg ctgaccgcca gtgaagaaca
     6961 ggcgcggatg gcaagcgaac gggaacagat ccgcaacgct ctgctggcgg cgctttcgca
     7021 tgatttacgc acgccgctca cggtgctgtt cggtcaggca gaaatcttaa cgctcgatct
     7081 ggcaagcgaa ggatcgcctc acgcccgcca ggccagcgag attcgtcagc acgtgctgaa
     7141 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7201 tttgaagaaa gagtggttaa cgctggaaga agtggtcggc agcgcgctgc aaatgctgga
     7261 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7321 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7381 gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga
     7441 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7501 tgctcgcggg aataaagagt cggcagtacc gggggtaggg cttggactgg caatttgtcg
     7561 ggcgatagtg gatgtacacg ggggcactat taccgcgttc aaccgaccgg aaggtggtgc
     7621 ctgttttcgt gttacacttc cccagcaaac tgcccctgaa cttgaagaat ttcatgagga
     7681 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7741 gcgctggagg gcgacgggat gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg
     7801 gaagcggcta cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     7861 gggattgagt ttatccgcga cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc
     7921 gcacgcagcg aagagagcga caaaatcgcc gcgctggatg ccggagcgga tgattatctg
     7981 agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8041 tccgccaccg ccgcgcccga tccgctggtg aaattttccg atgttaccgt cgatttagcc
     8101 gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca cgccgattga gttccgcctg
     8161 ctggcggtgc tgctgaataa tgccgggaaa gtgctcaccc agcgccagtt actcaatcag
     8221 gtgtgggggc caaacgcggt cgaacacagt cactatttgc gtatttatat gggacatctg
     8281 cgacaaaaac tggaacagga tcccgctcgc ccccgccatt tcattactga aac
//
LOCUS       DAPPNJ010000188         8314 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000188 DAPPNJ010000000
VERSION     DAPPNJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.190"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..565)
                     /locus_tag="RPM73_003669"
     CDS             complement(<1..565)
                     /locus_tag="RPM73_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDX6521982.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGA"
     gene            complement(767..1471)
                     /locus_tag="RPM73_003670"
     CDS             complement(767..1471)
                     /locus_tag="RPM73_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDX6521983.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1608..2060)
                     /gene="moaE"
                     /locus_tag="RPM73_003671"
     CDS             complement(1608..2060)
                     /gene="moaE"
                     /locus_tag="RPM73_003671"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDX6521984.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2062..2307)
                     /gene="moaD"
                     /locus_tag="RPM73_003672"
     CDS             complement(2062..2307)
                     /gene="moaD"
                     /locus_tag="RPM73_003672"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDX6521985.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2300..2785)
                     /gene="moaC"
                     /locus_tag="RPM73_003673"
     CDS             complement(2300..2785)
                     /gene="moaC"
                     /locus_tag="RPM73_003673"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDX6521986.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2788..3300)
                     /gene="moaB"
                     /locus_tag="RPM73_003674"
     CDS             complement(2788..3300)
                     /gene="moaB"
                     /locus_tag="RPM73_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDX6521987.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHLV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3322..4311)
                     /gene="moaA"
                     /locus_tag="RPM73_003675"
     CDS             complement(3322..4311)
                     /gene="moaA"
                     /locus_tag="RPM73_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDX6521988.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4299..4443)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4708..5616
                     /gene="yvcK"
                     /locus_tag="RPM73_003676"
     CDS             4708..5616
                     /gene="yvcK"
                     /locus_tag="RPM73_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDX6521989.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQTLG"
     gene            complement(5808..7829)
                     /gene="uvrB"
                     /locus_tag="RPM73_003677"
     CDS             complement(5808..7829)
                     /gene="uvrB"
                     /locus_tag="RPM73_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDX6521990.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLLSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDSVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         2059 a         2205 c         2058 g         1992 t
ORIGIN      
        1 ttgccccagg aacgatacag cgaagagaac ctgaaatttc agaggtgtcg gcaacacgga
       61 tcaaatcttc cagtgttgtt gcttcacagg cagccgtaat gccccagata aagacagtca
      121 aaatactcgc taaccacacg gaaaaactgc tggtaaggat caggttaaca acaaaagcaa
      181 tactcaaacc acagaaaata tatgaatata actgtctgac tggtgggata accctattac
      241 tacaagagca aatcagcgcc attgcggcaa acatccctcc cgtaattccc attaccgtcg
      301 ctatgctcga aaaggtataa aggctggcga agagaaagcc aacaagaagg attattagca
      361 gatagaccag acatgcgcct gatacattca gtcttttcag ggcgttgttt aaccagagac
      421 cacctattat cagcgcagcc aaagcgaaga caaacgcacc tggagacttt tgtgacgtaa
      481 cccaatgcaa atgctgaaat atccaggcac agcctgccgt agcccatact ccagctgctg
      541 ttaaccacca aatgagaagc agcattttgc tgagcgaagc acttttttgc tgtaattgag
      601 tagcagtgga ttgtctgttc aaatgcgtta tctgtccctg aaattacgta gtggacatag
      661 tctggcattt gccgcgattc agttctcatg taaatgagcg gaatagtctt tataaaatcc
      721 gaaaaaaatc ccggctccag gccgggatca gcaggaagaa gagaatttaa cggcggttgc
      781 cgaagatccg caacaacatc aggaacaggt tgatgaagtc cagatacaag gttaacgcgc
      841 caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc tcacccatat
      901 ttttcagttt ctgcgtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg
      961 taactgccca catcaatgct tcgcttttca accagaagtt gaccagcgat gccagcacaa
     1021 tgccgattaa cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt
     1081 aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta ctggcgatag
     1141 aagcagcggt atagacaatg aatatactgg aaagcgtaag acccgtcagc gccgaataaa
     1201 gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct gataacacaa
     1261 taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta gtgaacaaca
     1321 gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgcggtc agcaacaagc
     1381 caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta
     1441 cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc
     1501 ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgt atgtactgag
     1561 cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta ccagcgtttt
     1621 gccgcctgct gatcgctctc ccgggcttca acccagcggt cgccttccgg cgtggcttcg
     1681 cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac
     1741 gcactgctgc gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc cggccataat
     1801 tccccgatgc ggtgaatcac agtgacgcgc cccagcggcc agcggttacg cgcttcatca
     1861 acaatttctg ccagtgcttt ttcagtcatc cccggatagt gttcgagggt taatgctttg
     1921 acgctgtcgc ccaggttatg gttgcgcacc ttaccagtaa aggtgactac cgcaccgtct
     1981 tcgtcacgct ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca
     2041 acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc
     2101 gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt
     2161 gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccatgtgct ggcgtaacgc
     2221 ttcaacagtt gggaaatccg cagccacttc ggttgcatct gttcccacca actcgcgcac
     2281 ctgggcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc
     2341 cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca
     2401 tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt tcgacaccgg
     2461 ttttcccggt caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct
     2521 gtaaattgac ttcaactttg ctgagcatca gcggatgaca gagcgggatc agatcccagg
     2581 tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg cctttgtggt
     2641 ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc
     2701 gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg
     2761 cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg
     2821 acgcgtacgg gcatccagct gcggcgcaat gatattttcc catgcggtac ggcacgcttt
     2881 ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg acgcccgcta ccgcacgaga
     2941 ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacata cggaacactt caccaaaacc
     3001 ttcaacttca cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag
     3061 gccagtaccc cccgtaatca acaccacttg tacatcgtcg ctggcgatcc acgcagatac
     3121 ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgagatggcc
     3181 cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt caccgcgacg
     3241 attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat
     3301 ctgatctctc cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt
     3361 gttgttttga tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc
     3421 ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc
     3481 accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt tgcaagtggc
     3541 gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat ccggatgaca
     3601 aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag
     3661 tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct cactgccctc
     3721 gcccgtttcc atcagttcga tgaaacgcag ctggataggg cggtgctgga tccagttcag
     3781 aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt
     3841 ctcaaaaccg gcctcaaatg cagcatcgat ccctgccatg acctggttga atttatcctg
     3901 cccggtaata gcgtgaaact ggcgggcgtc cagactatca acgctgacgt tgatgccagt
     3961 aagtccagca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac
     4021 cgcaatctgg cggatagcgt cattttctcg cacagcggcg atgatatcgg taaagtcgcg
     4081 gcgtaaagac ggctcaccac ctgtcagacg gactttttcg gtgcccagac tggcgaaggc
     4141 gcgtgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga cgccgctcgg
     4201 tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag
     4261 gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt
     4321 tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat
     4381 tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt gtttagtggt
     4441 taagccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct atgtaatcat
     4501 gtatatagcg tcacgatcgg gctatttcgc tacagaataa gggaaaatgg cggttttact
     4561 ttgatataca tcatcaggaa ggcatagcgt acatcgtcct ttagaggtag aagaaaatag
     4621 tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc
     4681 gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga ccttgatcgt
     4741 gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg
     4801 ggttctcgtt taacggggat cgtcaccacc acggataatg gcggatcgac ggggcgtatt
     4861 cgccgctcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa ccagctgata
     4921 actgaaccca gcgtcgcttc cgcaatgttt gaatatcgtt tcggcggtaa tggcgaactt
     4981 tccgggcaca accttggaaa cctgatgctg aaggcactgg atcaccttag cgtgcgacct
     5041 cttgaagcaa tcaatctgat tcgcaatctg ctgaaagtgg atacgcattt gattccaatg
     5101 tcagagcatc cggtggatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag
     5161 gtcaatatcg accagttaac tacgccgatt caagagttat tgttaacgcc taatgtaccc
     5221 gcaacgcgtg aggcggttca cgctatcaat gaagcggatc tcatcattat tgggcctggc
     5281 agtttttata ccagcctgat gccaattctg ctgctgaagg aaatcgccca ggcattacgc
     5341 cgcacgccag cgccgatggt ctatatcggc aatctggggc gagagttgag tttacctgcg
     5401 gctaatttga agctggaaag caagctggca attatggaac agtatgttgg gaaaaaggtc
     5461 attgatgcgg tgattgttgg gccaaaagtg gacgtctcgg cggtgaaaga gcggattgtg
     5521 attcaggagg tactggaggc cagcgatatt ccgtatcgtc atgaccgcca gctgctgcat
     5581 agcgcgctgg aaaaggcatt acagacttta ggttaacgga aatccgaagg aaaattccgg
     5641 cttcctattg aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc
     5701 ggcctacaaa ccgcgctaat tcaatgtatt gcagagatca tgtaggcctg ataagcgtag
     5761 cacatcaggc aattttgctt ttgtcatctg tcttcttcgt tatcttgtca cgaagccgca
     5821 atgaacaatt cacgcagctg atgcaactgg tcacgaattt gtgccgcttc ttcgaactcc
     5881 agattctgcg cgtgttgcat catcaaccct tccagttcat ggattttctg ctgcagcgct
     5941 ttaggcgaca tatccatcgg cacactatcc ggctcaacaa tcgggcgcga ttttcctctg
     6001 cccttcgctt tggttttggc gatgttctgc cccagcgcca ggatatcgac cactttcttg
     6061 ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt ctcgcggcga
     6121 cgttcggttt cgccaatcgc tttcgccatt gatggggtga tcttatcgcc gtagagaatc
     6181 gctttaccgt taacgttacg tgccgcacga ccaatggtct ggatcaacga acgttcggaa
     6241 cgcaggaagc cttctttgtc agcgtcgagg atcgccacca gcgacacttc cggcatatcc
     6301 agaccttcgc gcagtaagtt gatccctacc agcacgtcga actcacccag acgcaagtcg
     6361 cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg cacgcgctcg
     6421 ccatgttctt cgagatattc ggtaagatct tccgccatcc gcttggtcag tgtggtgacc
     6481 agtacgcgtt cgttaattgc cgctcgctga cggatctccg aaagaagatc atcaacctgt
     6541 gtcgccaccg gccgcacttc gataatcggg tcaagcaatc cggttggacg caccacctga
     6601 tccaccacat cgccgccgga tttttccagc tcgtaattac ccggcgtcgc cgaaacatag
     6661 atagtttgcg gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt atccagcgct
     6721 gatagcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc accgcgatac
     6781 atgccgccaa tttgtggaat ggtgacgtga gattcatcga ccaccagcag cccatcggca
     6841 gggaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga gaggaagcgc
     6901 gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag atcaaactgg
     6961 gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaacag cactttgcgt
     7021 ctggcggcca gctcttcttt gatctcctcc atcgcctgta cgatgcgctc gcgcggtgtg
     7081 acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag tggaaacaat ctgcccggtc
     7141 agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac gcgaagtgca
     7201 atgtcatccg attctgccgg gaagatatct atcacctcgc cacgaacgcg gaaagtacca
     7261 cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg cagaatcgcg
     7321 cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata taaatcagga
     7381 tcgcccagac cataaatcgc ggaaacagac gccaccacaa ccacatcacg ccgctccagc
     7441 atcgctttgg tggcggacaa acgcatctgc tcaatatgtt cgttaaccga ggcatctttc
     7501 tcaatgaaag tgtcggaact cggtacatag gcttccggct gatagtagtc gtagtaggaa
     7561 acgaaatatt ccaccgcgtt ttccgggaag aactctttca tttcgccata cagctgggcc
     7621 gccagcgttt tgttgggcgc aagtaccatg gttgggcgct gaaggtcagc aatgacattg
     7681 gcaatggtga atgttttccc ggagccagtc acgccaagta acgtctggtg cgccaggcca
     7741 tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga aggtttaaaa
     7801 gcggaattca gtttgaacgg tttactcatg agtcgctacc tgaaggagtt gggcgggcag
     7861 gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga taaaaaaaca
     7921 gttcatcacc ataatatttc tgttacagcg taaactccgc tgtcaatcat gagcaaaatt
     7981 tactctgtgg cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt
     8041 taacatttgt caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg
     8101 ccattgcatt tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg
     8161 ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca
     8221 catggttatc cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg
     8281 caattcccac attcgccgca tgaggcggaa gtaa
//
LOCUS       DAPPNJ010000189         8313 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000189 DAPPNJ010000000
VERSION     DAPPNJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.191"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            210..980
                     /locus_tag="RPM73_003678"
     CDS             210..980
                     /locus_tag="RPM73_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDX6521991.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            971..1444
                     /locus_tag="RPM73_003679"
     CDS             971..1444
                     /locus_tag="RPM73_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDX6521992.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1551..2090
                     /gene="dnaT"
                     /locus_tag="RPM73_003680"
     CDS             1551..2090
                     /gene="dnaT"
                     /locus_tag="RPM73_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDX6521993.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2093..2830
                     /gene="dnaC"
                     /locus_tag="RPM73_003681"
     CDS             2093..2830
                     /gene="dnaC"
                     /locus_tag="RPM73_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDX6521994.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2879..3373
                     /locus_tag="RPM73_003682"
                     /pseudo
     CDS             2879..3373
                     /locus_tag="RPM73_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
     gene            3627..5918
                     /gene="opgB"
                     /locus_tag="RPM73_003683"
     CDS             3627..5918
                     /gene="opgB"
                     /locus_tag="RPM73_003683"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDX6521995.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHIDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6057..7079)
                     /gene="lgoD"
                     /locus_tag="RPM73_003684"
     CDS             complement(6057..7079)
                     /gene="lgoD"
                     /locus_tag="RPM73_003684"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDX6521996.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIQGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGDSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7218..8132
                     /locus_tag="RPM73_003685"
     CDS             7218..8132
                     /locus_tag="RPM73_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX6521997.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2105 a         2154 c         2022 g         2032 t
ORIGIN      
        1 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
       61 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      121 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      181 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      241 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      301 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      361 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      421 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      481 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
      541 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgt gcctgtttct
      601 gtaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
      661 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacaaatc aacttttgcc
      721 ttaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
      781 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
      841 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
      901 tcgccagtct gctgacactg gctacctgcg tcggcgtagt gatggcactg acgatttggg
      961 ggctacgcgg atgggtgtga tcgaatttct gttagcgttg gcgcaggata tgatcctcgc
     1021 cgccattcct gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cgttacgctg
     1081 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1141 gaatattgag tggtcaactt ttatggcttc tatgctggtc ggcaccattg gtattcaatg
     1201 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1261 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1321 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1381 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1441 ataaaattgc ctcgcttaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1501 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     1561 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     1621 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     1681 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     1741 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     1801 tccccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgac tttatccgcc
     1861 tggcggcgct atggggcgtg gcgctaagag agccggtgac caccgaagaa ctggcctcat
     1921 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     1981 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2041 atacggtcag cgaacctgac agccaaattc caccaggatt cagagggtaa cgatgaaaaa
     2101 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2161 caaaacgggt gaagagctac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2221 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2281 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2341 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2401 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2461 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     2521 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     2581 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     2641 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     2701 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     2761 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     2821 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     2881 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     2941 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3001 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3061 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3121 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3181 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3241 tcagggtttg ctgaaaacca aagatggtca gtaactcaat ctggataaca tcggaacgac
     3301 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3361 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3421 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3481 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     3541 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     3601 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     3661 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     3721 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     3781 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     3841 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg
     3901 gtgcgctggg ctggatcctg cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc
     3961 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4021 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4081 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4141 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4201 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4261 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4321 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4381 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4441 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4501 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     4561 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     4621 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     4681 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     4741 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     4801 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     4861 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     4921 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     4981 gacttggtcg tagcagttta tccgggcagt caatgtcgga aatcttcctc aatatcaaag
     5041 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5101 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5161 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5221 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5281 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5341 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatattgac aaaacaacat
     5401 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgctat aaaggcaatg
     5461 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     5521 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     5581 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     5641 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     5701 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     5761 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     5821 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     5881 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     5941 cataaggtgc gacctacaca ccttctccgg cctacaaaac atcattgaga aacagaaaaa
     6001 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6061 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6121 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cggcagtgat ttttccttcc
     6181 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct gcccatcatc
     6241 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6301 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6361 ttacctgtcg cgtcgatcac tttctgcgcc agcgaatcac caaactgcgc ccgtagctgc
     6421 gcgtcaaaat cttcggctga cgggtccagt acaggtaatt ccagacgcgt tgccacatgt
     6481 tcacggcgcg ctggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     6541 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     6601 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     6661 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     6721 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     6781 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     6841 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     6901 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     6961 caaatcccga cagacttaat ttttattaat gcttcattgt caccctgaat aggtatctcg
     7021 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7081 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7141 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7201 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7261 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7321 cggaaatgta caacatcagc cgcacaacag tgcgtcatat tctcagccac ttacgcgaat
     7381 gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7441 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7501 ttttcacgat gataaaccag cgtcagttac gccctgggga aactttttct gaactgcaac
     7561 tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta aaattcggcc
     7621 gttataatct tattcaaagc gaaaaacgcg gccagtggag catgaagcaa ttcgatcaat
     7681 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     7741 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     7801 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     7861 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     7921 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     7981 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8041 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8101 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8161 cgggtgcacg gttttatcaa aagataaata ccgagaaatt cccgcttacc tatgctcaca
     8221 atcagtcgac taaaaatttt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8281 ttaatgtcaa ccttgcgtaa ttcacaatta cgt
//
LOCUS       DAPPNJ010000190         8165 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000190 DAPPNJ010000000
VERSION     DAPPNJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.192"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            6..743
                     /gene="ygaZ"
                     /locus_tag="RPM73_003686"
     CDS             6..743
                     /gene="ygaZ"
                     /locus_tag="RPM73_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDX6521998.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            733..1068
                     /gene="ygaH"
                     /locus_tag="RPM73_003687"
     CDS             733..1068
                     /gene="ygaH"
                     /locus_tag="RPM73_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDX6521999.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            1159..1689
                     /gene="emrR"
                     /locus_tag="RPM73_003688"
     CDS             1159..1689
                     /gene="emrR"
                     /locus_tag="RPM73_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDX6522000.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            1816..2988
                     /gene="emrA"
                     /locus_tag="RPM73_003689"
     CDS             1816..2988
                     /gene="emrA"
                     /locus_tag="RPM73_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDX6522001.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            3005..4543
                     /gene="emrB"
                     /locus_tag="RPM73_003690"
     CDS             3005..4543
                     /gene="emrB"
                     /locus_tag="RPM73_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDX6522002.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(4607..5122)
                     /gene="luxS"
                     /locus_tag="RPM73_003691"
     CDS             complement(4607..5122)
                     /gene="luxS"
                     /locus_tag="RPM73_003691"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDX6522003.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(5272..6828)
                     /gene="gshA"
                     /locus_tag="RPM73_003692"
     CDS             complement(5272..6828)
                     /gene="gshA"
                     /locus_tag="RPM73_003692"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDX6522004.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(6901..7329)
                     /locus_tag="RPM73_003693"
     CDS             complement(6901..7329)
                     /locus_tag="RPM73_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6522005.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(7326..7892)
                     /gene="yqaB"
                     /locus_tag="RPM73_003694"
     CDS             complement(7326..7892)
                     /gene="yqaB"
                     /locus_tag="RPM73_003694"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDX6522006.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1912 a         2181 c         2056 g         2016 t
ORIGIN      
        1 actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc atggaaggat
       61 gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg ttcggtctga
      121 atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc atcatttatg
      181 caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt ttgtgggttg
      241 ctgcactgac cgtcatggca atggatgttc gccatgtgtt gtatggcccg tcactgcgta
      301 gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcattt ggcctgacgg
      361 atgaggtttt tgccgccgca accgcaaaac tggtacgcaa taatcgccgc tggagcgaga
      421 actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt acggtaatag
      481 gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagct gcattaggtt
      541 ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc aaacaatctc
      601 tttgcgttac cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt tctattcccg
      661 tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag gcattctggc
      721 aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg cgtggcgaat
      781 tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt
      841 ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt
      901 gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac gctggtcggc
      961 ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc aacactgctt
     1021 agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc cacaagaatc
     1081 atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat cggctgaaat
     1141 taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat gctaaaattt
     1201 cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg tctttgcatg
     1261 cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca ggggattaac
     1321 gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag tattcagcct
     1381 tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa
     1441 ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat
     1501 ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc gcagcataac
     1561 tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct cgagcaaatc
     1621 acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt tctcgaagcg
     1681 atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc
     1741 agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg agttaagaag
     1801 atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca accggtaaag
     1861 aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat aattattgcc
     1921 gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca
     1981 tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc
     2041 tgggccgata acaccgattt tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca
     2101 gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt tcgccaaacc
     2161 caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca aaaaatcgcc
     2221 ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt
     2281 ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc gcaactggac
     2341 gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa actggaagat
     2401 cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctggctggc gctggagcgt
     2461 acccgtattg tcagtccgat gaccggttat gtctcccgcc gcgcggtaca gcctggggcg
     2521 caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat gtgggtggat
     2581 gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt cactatcacc
     2641 acggatattt acggcgatga tgtgaaatac accggtaaag tggttggtct ggatatgggc
     2701 acaggtagcg cattctcact gcttccggcg caaaatgcga ccggtaactg gatcaaagtc
     2761 gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac agctggagca atacccgctg
     2821 cgcattggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg tcaggtactg
     2881 gcaaataaag tacgttcaac accggtcgcg gtaagtaccg cgcgtgaaat cagcctggca
     2941 cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc cagaggtgcg
     3001 tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga cgattgcgct
     3061 gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg cgatccccac
     3121 tatcgccggg aatctgggct catcgctcag ccagggaacg tgggtaatca cttctttcgg
     3181 ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg tcggggaagt
     3241 gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc
     3301 cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg ccgggccgtt
     3361 gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac gctcgatcgc
     3421 gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg
     3481 ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc cgattggcgt
     3541 ggcggtggtg ttgatgacac tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg
     3601 gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc tgcagattat
     3661 gctcgaccgc ggtaaagagc tggactggtt ctcatcacag gaaattatca ttcttaccgt
     3721 ggtggcagtg gtggctatct gcttcctaat tgtctgggag ctgaccgacg ataacccgat
     3781 agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct
     3841 cgcgtatatg ctctacttcg gcgcgattgt tctgctgccg cagttgttgc aggaggtcta
     3901 cggttacacg gcaacgtggg caggtctggc ctctgcgccg gtagggatta ttccggtgat
     3961 cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc tggtaacctt
     4021 cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg aaccaggtat
     4081 ggattttggc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg cctgcttctt
     4141 tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg cggcggcatc
     4201 gagcctctcc aactttacgc gtacgctggc ggggtctatc ggcacgtcca tcaccaccac
     4261 catgtggacc aaccgcgagt cgatgcacca cgcacagtta accgagtcgg taaacccgtt
     4321 taacccaaac gcccaggcga tgtacagtca actggaaggg cttgggatga cgcaacagca
     4381 ggcctccggc tggattgccc agcagatcac caaccagggg ctgattattt ccgccaatga
     4441 gatcttctgg atgtcagccg ggatattcct cgtcctgctg gggctggtgt ggtttgctaa
     4501 accgccattt ggcgcaggtg gcggcggagg cggtgcgcac taagtacaac taagccagtt
     4561 catttgaact ggcttttttc aattaattgt gaagatagtt tactgactaa atgtgcagtt
     4621 cctgcaactt ctcttccggc agcgccagtt cttcgttgct gttgatgcgc acgtcacgtt
     4681 ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg taagtgccac
     4741 actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc acgtcttcca
     4801 ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc agactcatat
     4861 aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca ttaccgttaa
     4921 gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct ctttctggca
     4981 tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg
     5041 gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta tgatcgactg
     5101 tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa attttctgaa
     5161 ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat catcatccct
     5221 gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct gtcaggcgtg
     5281 tttttccagc cacaccgcaa acggttcggt atctgcggct tccatttcct gctgacggcg
     5341 ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc
     5401 acgcagcaga ttacggtacg cttcagcaaa cgctttgcct gttccgccaa taccagtatc
     5461 aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga agcaggcaac
     5521 cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca gcgtttgcgc
     5581 gacgcgtttc agatcgcgga acagatcttt acccacctgc agtaacggga actgcgcggt
     5641 ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcaaggatca cccggttcca
     5701 gttaacgcgc gtacaggcaa gttcgctact gctcatttcc ggtgcatcag ccagcgcaca
     5761 ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa
     5821 cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta acagcgcatc
     5881 agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcat acagttcgtt
     5941 ttcaatctgc agcacgttgc tattgatttg cagcctctta ccgtctttct caataccaat
     6001 cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta cgtactcgta
     6061 aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc cgagatcgct
     6121 caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt tctcaaacgg
     6181 cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg caccaaacag
     6241 ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat agcccgcaga
     6301 aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc agaatgccat
     6361 tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt
     6421 tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc
     6481 cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc acatccgctc
     6541 atcacccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca gcatatgttc
     6601 aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat cggtagtaat
     6661 ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc
     6721 atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg
     6781 aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg
     6841 cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc aggcctacaa
     6901 ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat agcgtaacgc
     6961 tttaccaagg cacaaaaaaa agattaccgg tccccacgag atgcgcatcc atcccgctaa
     7021 cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga ctgcaccata
     7081 gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca atggaaagaa
     7141 acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg ctgttaaaac
     7201 taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga cttcggagtt
     7261 gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa gcgataacgc
     7321 ttcactcaca gcaagcgaac atccacggcg tccatgcctg ctgcacgggc cgcctgaata
     7381 ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg
     7441 cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcagc agcgacgacg
     7501 gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg cctcagcgat ggcgctttca
     7561 ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac ttcaacaaga
     7621 ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt ttcacgcgct
     7681 aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc aatacgccag
     7741 gtgggcgatc cattaagcgc aatcattgcc tgaacatcgt actgaagacc gtagtgccct
     7801 aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggattgt gccatccata
     7861 tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga aaaatatgac
     7921 gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga aaattcagga
     7981 aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt gggagaagat
     8041 ggtgcatccg ggaggattac tcactggcgt tcgcccttcg ggccgttgct gacgcaacgt
     8101 tatcctccct ggtgcctgcg gttaataccg ctatctcttc gataccttca ttgctgaagg
     8161 ttacg
//
LOCUS       DAPPNJ010000191         8148 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000191 DAPPNJ010000000
VERSION     DAPPNJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.193"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..305
                     /locus_tag="RPM73_003695"
     CDS             <1..305
                     /locus_tag="RPM73_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522007.1"
                     /translation="EKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPT
                     KDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            528..1025
                     /gene="ubiC"
                     /locus_tag="RPM73_003696"
     CDS             528..1025
                     /gene="ubiC"
                     /locus_tag="RPM73_003696"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDX6522008.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1038..1910
                     /gene="ubiA"
                     /locus_tag="RPM73_003697"
     CDS             1038..1910
                     /gene="ubiA"
                     /locus_tag="RPM73_003697"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDX6522009.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2065..4488)
                     /gene="plsB"
                     /locus_tag="RPM73_003698"
     CDS             complement(2065..4488)
                     /gene="plsB"
                     /locus_tag="RPM73_003698"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDX6522010.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4659..5027
                     /gene="dgkA"
                     /locus_tag="RPM73_003699"
     CDS             4659..5027
                     /gene="dgkA"
                     /locus_tag="RPM73_003699"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDX6522011.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5137..5745
                     /gene="lexA"
                     /locus_tag="RPM73_003700"
     CDS             5137..5745
                     /gene="lexA"
                     /locus_tag="RPM73_003700"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDX6522012.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5818..7143
                     /gene="dinF"
                     /locus_tag="RPM73_003701"
     CDS             5818..7143
                     /gene="dinF"
                     /locus_tag="RPM73_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDX6522013.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEILKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7259..7468
                     /locus_tag="RPM73_003702"
     CDS             7259..7468
                     /locus_tag="RPM73_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDX6522014.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7510..8025)
                     /gene="zur"
                     /locus_tag="RPM73_003703"
     CDS             complement(7510..8025)
                     /gene="zur"
                     /locus_tag="RPM73_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDX6522015.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1833 a         1995 c         2237 g         2083 t
ORIGIN      
        1 aagaaaagtt aagaaccttt aacactaata aattaacacg aaacgataat atatttatcc
       61 tgttctcctc tgtcgatgat gggcctgtta tggtggtaag ctcccagcgc ttacatgata
      121 tgttgaatcc tacaaaagat accaattgga attccacgta tatctacaaa tccagacatg
      181 agatgttgcc tgttaatctt actcaggaaa cacttttcag ctccaaatct catggtaaat
      241 atgcgctttt ccccattttt actgcgagtt ggcgagctca tcgtataatg aataagggtg
      301 tttaagtaaa ggaaaacatc accgttcctg gcatcctggg cggtgatgcc cctatggttg
      361 ccctctccag cgcgggcatc ggcaaagcgt aaggttcaac agcgttttac cacttcatgc
      421 gattgttgcg tttttgttgc gtattagatc acttaatttg ctttacatct cccgtaaaca
      481 cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg tcacaccccg
      541 cgttaacgca actgcgtgcg ctgcgctatt ttaaagagat ccctgccctg gaaccgcaac
      601 tgctcgactg gctgttgctg gaggattcca tgacaaaacg ttttgaacag cagggaaaaa
      661 cggtaagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc cccgaagaac
      721 tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta tgtgccgatg
      781 gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc gggccggagc
      841 tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca tcatcgacat
      901 taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga cgttcccgcc
      961 tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt
     1021 actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg gcgtttcatc
     1081 gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca acattatggg
     1141 cgttgtgggt ggcgacaccg ggcgttcctc agctctggat cctggcggta tttgtcgcgg
     1201 gtgtctggct gatgcgcgct gctggatgtg tggtgaatga ttatgctgac cgaaagtttg
     1261 atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta acagagaaag
     1321 aggcgcgcgc gctgtttgta gtgctggtac tgatttcgtt tttactggta ctgacgctga
     1381 atacgatgac cattctgttg tcgattgccg ctctggcgtt ggcgtgggtg tacccgttta
     1441 tgaagcggta tacccatctc ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc
     1501 caatggcttt tgccgctgtg agtgagtcgg tgccactgag ttgctggtta atgttcctcg
     1561 ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac cgcgatgatg
     1621 atgtgaagat tggcattaaa tccacggcaa tcctgttcgg ccaatacgat aaattgatta
     1681 ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag ttaaatggct
     1741 taggctgggg atattactgg tcaattgtgg tggctggcgc gctgtttgtt tatcaacaaa
     1801 aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat aataactatg
     1861 ttggtctggt actgttttta ggactggcaa tgagttactg gcatttctga tgatgtaaaa
     1921 aagccggatg atcatccggc tttcttctgg gttgccttat gcgcggcgct tctcaggcct
     1981 acacaacaca tcgcaattta ttgaatttgc agattatgga aggccggata aggcgttttc
     2041 gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa
     2101 acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg tttctgccgg
     2161 ttcggcgtcg ccgctatcgc tgatataccc ttcatcacgc agcgtcagca ccagagaact
     2221 gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg agagacgttg
     2281 cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt tggcactcaa
     2341 caaccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg ccagcagctg
     2401 tagggtgcga gaatgcgccg ggttgatatg caactcatca tcttgcaggg taatcagccc
     2461 ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt cgcgatccca
     2521 gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac
     2581 gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag gcagcaccaa
     2641 catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc gtggcagaat
     2701 gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca gcgcgtgatc
     2761 gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag gtacgttgcg
     2821 catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg
     2881 acgtgatgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc ctgcgttgtt
     2941 aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc aggccggacg
     3001 caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt taaggtaggt
     3061 catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac gcagcttgct
     3121 taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc gcagttcttt
     3181 ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa tcagcgtaat
     3241 cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac cggttttcgg
     3301 atccagcaaa cgccccgtgc gggaacgacc gccttccacg aagtactcga cggaataacc
     3361 acggctgaac agttcgccga gatactcacg gaaaacggtg gaataaagtt tattgccttt
     3421 aaaagtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg ccggccagaa
     3481 gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtggt acagcacgta
     3541 agaaagtagc aggtagtcca tgtgactgcg gtggcaaggc acatacacca gctcatgacc
     3601 gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct gataaagtcg
     3661 gttccaggtg aagcccagaa tgcggtcagt caggcggatc atctcgtaag agaaattcgc
     3721 cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg agattttttt
     3781 gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca gcttattaaa
     3841 cagatcctga cgagcaggca gacgtgggcc cacagcagcc agacgttgac gggcaaagtg
     3901 catacgcgcc acgcgcgcca gtttctgagc gatagtttta tctgtgccgt gttcatcggc
     3961 catgcggcgc agagaaactg acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag
     4021 tacggcgaaa aatttttgta cgccgttaag catacgcagc ggcgggttta cttcgccttt
     4081 ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca catccagatt
     4141 tgggttgctg cggtgcaaat caagatagtc gtggaacagc ttaatagact cttctttcgg
     4201 cgtgtaatag gtgaacacac gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt
     4261 gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgagccagac actgggcgcg
     4321 caacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg gacgagaggt
     4381 atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta ccaggatgct
     4441 taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa acgatgtaaa
     4501 gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg gaaattctgt
     4561 ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat ttaacggtac
     4621 actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat accactggat
     4681 tcacccgaat tatcaaagct gctggctatt cctggaaagg tttacgcgct gcatggatca
     4741 acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct
     4801 ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga
     4861 ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc tctgaatacc
     4921 atgagctttc cggacgcgca aaagatatgg gatccgccgc ggtgctgatt gccattatcg
     4981 tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc ttccagattt
     5041 cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt gctgtatata
     5101 ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac ggccaggcaa
     5161 caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt
     5221 gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag
     5281 gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg
     5341 ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccgctt
     5401 ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt caagccgaat
     5461 gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggt
     5521 gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt
     5581 attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg
     5641 ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt
     5701 gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtaacatat ctctgagacc
     5761 gcgatgccgc caggcgtcgc ggtttgtttt tcatctctct tcatcaggct tgtctgcatg
     5821 gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat gattttctcc
     5881 aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca tcttgatagc
     5941 ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct ctttatgctg
     6001 ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg tgccaaaaat
     6061 cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg ggctggggcg
     6121 ttaattgcgc tgctgcgtac accgattatc gatctggcgc tgcatattgt tggcggtagt
     6181 gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag cgctccggcg
     6241 tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc ccgtgcgcca
     6301 gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg gctggtgatg
     6361 gggctacata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca
     6421 ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat ttccggcgaa
     6481 atactgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa ccgcgatatc
     6541 atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact tggtgcgcga
     6601 ctggggagtg acattatcgc tgttaacgcg gttctgatga cgctactcac ctttaccgcc
     6661 tatgcgcttg atggttttgc ctacgcggtt gaagcgcact ccggtcaggc atacggtgcg
     6721 cgcgacggta gccagttgct ggatgtatgg cgggcagcgt gccgccagtc ggggattgta
     6781 gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc gttgctgacg
     6841 tcgttaaccc agattcagca gctggctgac cgctatctta tctggcaggt gattttgccg
     6901 ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac gcgtgccgcc
     6961 gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct ccttacgctg
     7021 ccgtggctgg gtaatcatgg tttgtggctg gcattaaccg tctttctggc gttgcgcggg
     7081 ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg
     7141 tgacggttaa aaattctgaa taaattatcc taagccaaat tgctgactac acttaatctc
     7201 acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg tcttgattat
     7261 gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga aagagcaatg
     7321 gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc aactggtcgg
     7381 taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg tcgtggattg
     7441 ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg tacaaggatg
     7501 tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct ggcactgttc
     7561 aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg cttcaatcac
     7621 attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcataatgt cttccacgcc
     7681 ttctgcacac tcttctttca ccgcgccgca gcgatcgcat ataaacatgg ctgacgtatg
     7741 cgtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca ccttatgcac
     7801 aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct tggcttgcgg
     7861 ttcagcttcg cgcagtaaat caagcagatc ataagcgctg atagcgccat cctgcagact
     7921 catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct gcgcgcagat
     7981 tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc acctcaaagt
     8041 gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga acccttattg
     8101 ttgccgtaat gttgattttc tgttttgtag gtaaggtgtt gtgttgcc
//
LOCUS       DAPPNJ010000192         8096 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000192 DAPPNJ010000000
VERSION     DAPPNJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.194"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(77..556)
                     /gene="folA"
                     /locus_tag="RPM73_003704"
     CDS             complement(77..556)
                     /gene="folA"
                     /locus_tag="RPM73_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDX6522016.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(748..2610)
                     /gene="kefC"
                     /locus_tag="RPM73_003705"
     CDS             complement(748..2610)
                     /gene="kefC"
                     /locus_tag="RPM73_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDX6522017.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(2603..3133)
                     /gene="kefF"
                     /locus_tag="RPM73_003706"
     CDS             complement(2603..3133)
                     /gene="kefF"
                     /locus_tag="RPM73_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDX6522018.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RRYKQRLLEWQEAHHG"
     gene            complement(3241..4572)
                     /locus_tag="RPM73_003707"
     CDS             complement(3241..4572)
                     /locus_tag="RPM73_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6522019.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(4631..4918)
                     /gene="fixX"
                     /locus_tag="RPM73_003708"
     CDS             complement(4631..4918)
                     /gene="fixX"
                     /locus_tag="RPM73_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDX6522020.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(4915..6201)
                     /locus_tag="RPM73_003709"
     CDS             complement(4915..6201)
                     /locus_tag="RPM73_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX6522021.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(6252..7193)
                     /locus_tag="RPM73_003710"
     CDS             complement(6252..7193)
                     /locus_tag="RPM73_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDX6522022.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNAKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(7208..7978)
                     /locus_tag="RPM73_003711"
     CDS             complement(7208..7978)
                     /locus_tag="RPM73_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDX6522023.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLIIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         1979 a         2361 c         1981 g         1775 t
ORIGIN      
        1 tgagcgcgac tcacaaaatc gcgctcactg gtaaaatcaa acgtcttatc gttcaatttg
       61 tagtcctggc aaaaaattac cgccgctcca gaatctcaaa gcaatagcta tgagagttct
      121 gcgcatcggc gtcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg
      181 ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttttgcgctt
      241 ttggcaggaa ctgctcataa acgcgaccac cgccaatcac catgatttct ggtacgtcac
      301 cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg
      361 gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccagg
      421 tatggcggcc cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg
      481 caggcaggtt ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg
      541 caatcagact gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc
      601 gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg
      661 agtccacgct ctctccgtgg actcgccgca ttacaatgaa acaaaaacaa acagttagct
      721 gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc
      781 catgttgccg gtatgtttac cttcttcggt tccctgccag ccatgtcgtt gaattaatga
      841 cagatgttcg cggtcctcgg taatgatctc acttaacatc gcgctggtgc gtttatagac
      901 cgccgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca tctgaatatt
      961 aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc
     1021 cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac
     1081 gcctgcctga cgcaaacgaa tgtagtggtc gacatcgcgg gcgcgggcaa taatctgcaa
     1141 atgcgggaaa tgttctttca ccatctctgt cagttgcagg ttggtttgcg gatcgtcgat
     1201 ggcgttaatc agcacttccg ctttcgccgc tccggcagat tccagtaaat ccatccgcgt
     1261 ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat ggtccggatc
     1321 gtgatcgagt accaccattt tcaccccgct ggagagcagt aaacgtccgg taatctgccc
     1381 aaaacgaccg aatccggcga taatcacgcg cggctgttct tcgtcgatct catcggcttc
     1441 acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt
     1501 tgctgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac
     1561 attcgccatc tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa
     1621 caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa tcagccacag
     1681 catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc
     1741 aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa
     1801 acctttaaat ggttcgatat cgctctccag cgcatgacgg tattccgagc ttgccagcag
     1861 tacgcccgcc agaaacgctc ccatcgccat cgacaagccg acctcttcca gcagcaaacc
     1921 aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc
     1981 tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca gcaccagcgc
     2041 acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgt
     2101 tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga acagcagcac
     2161 cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca tcgcctgcat
     2221 ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg
     2281 cagcccaagt aacatgcaga acagccccag caggccaccg caaatcacca tctgcaatgc
     2341 gccaccgccg aacactgccg cacgcagctt ccacagcctt tgtggatcga gttcgaggcc
     2401 gataataaac agcatcagca ccacgccaat ctcggcaaag tgcagaatag attcggcatc
     2461 ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcgatcaggt agccaagtac
     2521 cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gccgccgaac cgaggtagat
     2581 cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt
     2641 gcttatagcg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtacagtgca
     2701 tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg
     2761 acagcacatc aaaacccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg
     2821 tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccatgaccg taagcccagc
     2881 cgtgggagaa aactttatcg atccaaagtt tgaggagcgg aggaatgctg taccactgca
     2941 tcgggtgctg ccagacaatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat
     3001 cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc
     3061 ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa
     3121 ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca
     3181 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaaa
     3241 ttaccccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acatccctcg
     3301 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
     3361 tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc aggtcgaaac
     3421 aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc ggatatctgt
     3481 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
     3541 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac
     3601 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
     3661 gtttaaccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt
     3721 ccccagcgcc gcgtttttgc caacacccaa tcccaacaaa ccaacgattt gcgggccaaa
     3781 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
     3841 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
     3901 atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
     3961 ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa
     4021 caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
     4081 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg cgataaagct
     4141 gatggaaaat gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg
     4201 ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga
     4261 tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
     4321 ttttcgccgt ccgactttat cggaaatata gccaaacagc gatgtgccaa cgaacagccc
     4381 ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggggt
     4441 cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc
     4501 gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct
     4561 ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacgg cgttacgtat
     4621 caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact
     4681 gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt
     4741 aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca
     4801 ccagcagctc cagcgcctgt ttatcggcat cggccttcac aacaatgtgc ggatgctctt
     4861 catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa
     4921 aacggtcact cctttcatgc catccttgat tagattgatg aagcccactt tcttgccatg
     4981 gcggaggatt ttcttgcgca tcagttccgg cgcgctgcca tcaatggtga acaggtcacg
     5041 cgccacaccc accgccagct ccgggtagcc gctaaacatg cgtgggttat caaggaacgc
     5101 cggtagtttc tgatacatac gcatatcgcg cagcggacca ctctcaagat gctgacgata
     5161 ttccgccagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc
     5221 tgcggcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgagattcat
     5281 acacattcca gcggcatcac cggcaatcaa tacgccgtca ccaaccaact ccggcagcat
     5341 gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc cacctgcgat
     5401 cagtggtgca acggccggat gttgtttgaa atcttccagc atttgcggca ccgatttttt
     5461 cgcgtcatgc agatgatgca aaccacaaac cagccccagc gacagggtgt tttcattggt
     5521 ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc
     5581 gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta actcgatcag
     5641 ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc ccagcttttc
     5701 ggcaaggatg gagttcactc catcagcaag gatcaccgtt ttcgcttcaa tcacatcgcc
     5761 atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc
     5821 ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc agccaggcat caaatttact
     5881 gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccattgc agtagtccat
     5941 agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac
     6001 gggggcggag tcggcgaaac caggaataat gtgttccagg ctgtgggcat agagacgccc
     6061 gccggtgacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc
     6121 ttcgcgggcg agcaccagtg cggcaaccga accggcaagc ccagcaccga cgatgatggc
     6181 gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagggccaaa atgcagccct
     6241 gccagagtgg atcaacgcgc taaagctgcg gtcagcgccg gaaggatctt cacggcgtca
     6301 ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg
     6361 aaaatggttt gcgatgcatt agcaccaacc atgtgctgga tctgcccgga gatccccacc
     6421 gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc
     6481 atccattttt cgttttccgc caccggacga gaacaggcca actccgcacc tatcgcctta
     6541 caaagctgtt ctgccagcgc aatgttatct ttgctgccaa tgccgcgacc gacgctgacc
     6601 accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg
     6661 gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg
     6721 tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gaaccgcata cggcgtggca
     6781 atgcgttctt cgccaatcgc cagaccaccg taaaccatat gtttcactgt tgctttaccg
     6841 tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt
     6901 tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca gcaccaggcc gtctgcgccg
     6961 tgctggcgaa tagtgtcagc catgacactg gcgtaatctt cgatcatccg atcgtccggt
     7021 ttggcgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca
     7081 tcattgagga caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc
     7141 ggcagacgag aaggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc
     7201 cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt
     7261 cgccttcaat cacgatgcgc tgacgttcgc gctgtttcgg cgcggcaacc tgttgttcag
     7321 accaggcgtc cacggcgtta aaaccgatat ccgccgccga ccatacctgg actggctttt
     7381 tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa
     7441 cagcaacaac cgcaggcagc ggaatgatta acgtttctgt ttcatcttcc agttcgcgct
     7501 caacggtgag ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa
     7561 tattgaggat ttcgcctacc agcaaaccaa cctgctgggc ataaaggtcg gaagaaccat
     7621 cgccacagag gatcaaatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg
     7681 tttgttgcgg cagtgcctgc tcgaactggt catcaattac cacaatcagt tcatccgggc
     7741 cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta
     7801 gggccgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt
     7861 tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa ccatcagcat
     7921 tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg
     7981 catctccaga aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt acagatatca
     8041 cgccctttaa tttggaaata aaactttaat caccaatatt gaaaatgtca cacgca
//
LOCUS       DAPPNJ010000193         8081 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000193 DAPPNJ010000000
VERSION     DAPPNJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.195"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(331..464)
                     /locus_tag="RPM73_003712"
     ncRNA           complement(331..464)
                     /ncRNA_class="other"
                     /locus_tag="RPM73_003712"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(499..575)
                     /locus_tag="RPM73_003713"
     tRNA            complement(499..575)
                     /locus_tag="RPM73_003713"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(539..541),aa:Pro,seq:cgg)
     gene            complement(667..2358)
                     /gene="eptB"
                     /locus_tag="RPM73_003714"
     CDS             complement(667..2358)
                     /gene="eptB"
                     /locus_tag="RPM73_003714"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDX6522024.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(2611..3135)
                     /gene="lpfE"
                     /locus_tag="RPM73_003715"
     CDS             complement(2611..3135)
                     /gene="lpfE"
                     /locus_tag="RPM73_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HDX6522025.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            complement(3141..4196)
                     /gene="lpfD"
                     /locus_tag="RPM73_003716"
     CDS             complement(3141..4196)
                     /gene="lpfD"
                     /locus_tag="RPM73_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HDX6522026.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            complement(4207..6738)
                     /gene="lpfC"
                     /locus_tag="RPM73_003717"
     CDS             complement(4207..6738)
                     /gene="lpfC"
                     /locus_tag="RPM73_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HDX6522027.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            complement(6762..7448)
                     /gene="lpfB"
                     /locus_tag="RPM73_003718"
     CDS             complement(6762..7448)
                     /gene="lpfB"
                     /locus_tag="RPM73_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HDX6522028.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            complement(7532..8056)
                     /locus_tag="RPM73_003719"
     CDS             complement(7532..8056)
                     /locus_tag="RPM73_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HDX6522029.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
BASE COUNT         2083 a         2038 c         1873 g         2087 t
ORIGIN      
        1 gctaaaacgc acagcggaaa atgccagcgc cagccataag taacgcaaaa aaagatttaa
       61 gcaaatataa aaaaaacaat ggtttctgtc acaaatccgt taatggatag tgagatatgg
      121 ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga ataaggcggt cgcgccgcat
      181 ccgacaataa gtgcggtgcc gttgcctgat gcgacgctgg cgcgtcttat caggcctacg
      241 tcgagtcgaa cctccctgcc cccggaggtt ctcatcctca tgggctacaa atgcaaaaaa
      301 agcctgatcg ctgagcaggc ttttcgaatt tggtcggtga tagaggatta ctcgccactt
      361 cgtggctcgc cctgcgggcc gttgctggcg cgacgttctc tcgcttcgct cgagtcgaac
      421 ctccccgccc ccggaggttc tcatcctcat gggcttcaaa tgcaaaaaag cctgctcgct
      481 gagcaggctt ttcgaatttg gtcggtgata gaggattcga acctccgacc ccttcgtccc
      541 gaacgaagtg cgctaccagg ctgcgccaat caccgaatgc ggggcgcatc ttactgcgca
      601 gatacgccct cgtcaatccc ttaatagcaa aatgcctttt gatcggcgag aaagtcagca
      661 ggccgcttag ttagccgctg ccacttttgt ctgcgggatg tgacaccagt tgttgttttc
      721 gttaattcca ccatccggtg aagtatagcc aagacaaccc atgatggtgt cgtacagctc
      781 tacatgacgg cgtggcactt tcatgtcggc ttcttttttc agctgcgcaa atgcctgagc
      841 attggccgga ttttccagat atttatctga catccagacc atcatcggta cgcggaactg
      901 ctccggcggt gccagttcac gcggcgtgcc gtgcaggtgc tcgcgttcat taattgactc
      961 accgtggtca gctgcgtaga acacaatcgc tttcttatcg cgaacctgat caatcacgct
     1021 ggagataaag tgatccacat aggtcaccga gttgtcatag gagttgatca tctgcgcttt
     1081 ggtacagccg ctatcaacac caatacattc cggcttccac tgcgcgaagc tacgcggata
     1141 acgctgggtg tagttaaaat gcgaaccttt ggtatgcaga atgatcagat gcttaccatc
     1201 cgggttgcgc cctagcgatt gctgcatttc gtctaccagc aacatatcat ctaccggctt
     1261 gccacgatta cgtggctccg caccaatctg ctcacgataa gcaatgttgt ccgccatcgt
     1321 gttgctgtag aaccacattt cgctctgcat agcgtagagg tcagaactga atcctaactg
     1381 cttcagaacc gcgaaaatgt tctgttcttt taatgtgcgc tgcggattat cttccgcgcc
     1441 cccctgacgt acaaacatgc aacgcagtga gagtttggtt gcggtatcac aggagtaacc
     1501 acggaacgcc gccagatttt tctcctgggc cagtttcggc gtggtattac gctcatagcc
     1561 gaaaatgccc atatggtccc aacgcgtggt ttcaccgatg ataaacacga cataagtgtc
     1621 atcaacgttt tgcggtgcct gataagtgaa tttcttcgcc ggattaagca atgaattatt
     1681 atcggaagat tcatcgaccc gcgcccaggc atacagcccc aacgcagaaa gccagtttga
     1741 tggcagataa gagttcgcta cgacaccgcc ataactcggc aaatcaacgc cggtcgccct
     1801 ctccactttc ttctgctgga tatccagcaa acgaatcggt gcccaaacca taatacccgc
     1861 cagtacgacg accgccaggc tgcgaatacg ctgccctggg gttcgcagtt gtcggagcaa
     1921 ggtgtagcga cagcggttat tccagataag gataagaggc aatgcactaa cggcgattaa
     1981 ccagagaata aagttcagac caacaacttc ttttgacagg tcgatatcgg tggtcatgac
     2041 ggaagcgatg atgccataac caatgaccac attaaggaag gtcatgtaat agctggcacc
     2101 tgcggaaaag agcaccacca gcgacgccag aatacgccag ctgcggcggc caaacagcga
     2161 tagaagacgt agtaaaaaga aggtcaccag tacggtggcg gccagttcaa caacagcaga
     2221 aacgcctttc cagacggtaa aatcgtgcgc atagctgccg aagcggcgat aaaaaaccgc
     2281 gccattcata aaaaggccaa tatagattgc aagcaaaaag ctcagcttct gctgtgtaat
     2341 cgatttgatg tatctcatgc aaacaaaccc tggacaaaca ggtgataaca ccctgcttag
     2401 cggcttttca ggcccggcag gggaaaagtg cggaggtagt cagataagta aaatctacac
     2461 gcgcgaataa gtagaccaca gcaaagcgaa aaagtgtggg gaaagagtgt gcatgaagca
     2521 caaatttaca aaaatgcaag tttgaaaaga gactttagcg ttacaaaaag cagtaaaaag
     2581 ggccagttaa ctggccctta agatgtgaaa ttattcgtac tgtacagtaa agtcgaccgt
     2641 tgcatctgcg cgaccaatag ttgcatcggc gcgaataggc acgtagctgg cgatatagtt
     2701 cagttgatgt gcgccatcac cgttaattgc aaactcaacc ggtgtcggcg ttgtattttc
     2761 cgggaactgt tgtactccag cagcggtttt gattttcagc ccaacgcctg tagcgccacc
     2821 cgcatccagc gcaaaaagat ttgagtcgtc tgcattgggt gtaccgtcca tagtcacgta
     2881 ggctctgacc acttcgctac agtttttcag gccgatggaa aaaggagtgg gattaatttc
     2941 cgttccgatt cttaccggct ttttgagaac cgcggtgccc atgtcaacct caacgctgtc
     3001 tttcaccagt tcgcaagcac cctggctgat tttaagggtg aatttcaatg ttcctttcgg
     3061 ccctaaatcc gtggttgccg cgaatgacga aaaggaaaga ggcagcaggc ataacgcaag
     3121 tattgttcgc ttcatgactg ttactccagt tcgacatgta tggtagcaat accgctataa
     3181 tccccggcag cgggtaattt tccggtagtg ctaacgggat aagcggtaat tgtggttgtc
     3241 gctttgcgtt tgacatcttc atacagcgag cctgggttca tttctaataa gctattactg
     3301 tcattcggct taagaatatt acctttaccg tcctcaatga ctgcaccaac atcggcgttg
     3361 cctaaatcaa ttgcgctggg ataggcagta tttggtgttg catcaatact gagataaatt
     3421 ttgacgccat ctgaaatatt attgcagtca aagcttaatt ctttttgtac cttctgaaca
     3481 ttttgtggtg gttgtccggc acgcccttta aaatcatggg cctgatattc tccgaatgga
     3541 atatccagta cggtgccagc ggcaagttca cagccttgag taactgtaat actcccggaa
     3601 atactgacat tggccatggg ctgactatca ttgtaaacac ctttcttttt tgtcccaaag
     3661 agggacgcaa ctcgtgtttt aggaataatc atttgcccaa caaaaggatg tgaaatataa
     3721 atactgatac ttcctttacc acctgtcccc caattgttag cagtataggg cgtacattct
     3781 ttctgtgccg tgccattgct tcgatcggta aaaggcaccg ggatatctaa ttgtgtgtag
     3841 acatatattt tggtgagaat atcaatgtaa ttattgaatt tatagtaccc atctacagtt
     3901 ccaggctcag gcagaagcgt tacaccttta aaataagtca caactccatt atctggaatg
     3961 ggatccgggc attcacaata agcggaatat tttgtaccta aagcaaaggg ctgagttaat
     4021 attttcccag ccttattttc tgaaggtacc tggatagtct gtacgaaatc aaaactatac
     4081 tcatgggcca ccccatttga tgtctgacat ggtccccaat ccgcagccca tatatttcca
     4141 gcaaaagtgc tcgtccctaa cagcaccata cctgccaaca aatttttaat tttcatctaa
     4201 atatccttat ttctcgcata ccgcattaac catcacgata gccgcattgt ccttgttctc
     4261 tggcaagcgg aatggcacac ggcaacgttg cgcctgctcg ttcccccagc tcacctgcaa
     4321 ttcgccctct tctggcatcc cgctgatata aagctgtcct tcttcaccca caatgctgct
     4381 ggcaggtttg ctctcatcaa ttaacgctgc ggttgcacca aacggtactg cgttgccatt
     4441 cgcctgtttt aagctcatca gcacacggta accgacgcgg gtatcaaagc gggaacgcac
     4501 aactgcacca cgagtaggca cgactttctg gaacgcttcc tgtagttcaa cgttttgccc
     4561 caacgtgtcg ctacgcaatt ccacaatggt ttcacgatat ggtgtgagat aaggcaccac
     4621 ggcgttgccc atccagtcaa cttctacgcc gctgctgtta accacatgtc caccacgcgc
     4681 gcccggtgca gaaacaatcg ccagactttc gcctaaaggc tgcgaaagcg ttataccttc
     4741 actgtgaacg atgacaccgc cgcgcacgcc atagttaacc tgttggttat ctttagaata
     4801 gttatagcct acgtcaatgt taccagagcc acccatatac cccatgttga cgctgccact
     4861 gtagcctacg ccattactgg tgtaaccctg ctgaacgtta tagctcaggt tacgatcttc
     4921 cagtaacgtg ccgctaacag aggcctgttg cgacgtcttg ccattctggt cggtagtcat
     4981 gcgataactg ctccacgcgc cccccagagg gatggaaaca ctgaatgacc acaagcgatc
     5041 gtcttcttcc cattccggac ttctcgtcca ggtataggcc acgctgtaat tcacccgacc
     5101 aatgtgaccg tgataaccgg cgcttaagga gttttcctta ccgtcaacgt tccagtaatc
     5161 ctgctgcgtc atattgaaat aaaccgagcc gtagtctccc agttgctggg taatattgcc
     5221 ctgtatctgg ctgcgcttgt gataacggcg gtaatcactg tcggcgtcac tgcgaacatc
     5281 agttgcttcc tggaaggtgt agtagccgct ggttgagtat ttgtagccca acagacgcag
     5341 gtcagtgcca ccctcaaaac ttttcgaata gaggaagcga taagactggc cttcctcttt
     5401 ttcattgttg cgtaactgac ttttcgcctg agtgacatcc accgatactg cgccaatggt
     5461 accgaagttt ttccccaggc ccagagctaa agcgctgtaa tctgccgaca acaatgcacc
     5521 accgtaggcg gtcatacccc acggcaagcc gtacatggcg gtaaactgcc cgaaatgtgg
     5581 tttatcgctg tcgtaattac cagcacgata ttcaccggca gccagactgt atttcagata
     5641 cccttcacgc tggaaaatcg ccaccgccga aaacggctgg ataaaacgtt gttcacttcc
     5701 gtcggcttct ttaatgatca ccgtcaggtc gccgctgccg gaggttgcgt agagatcgtt
     5761 aatctcaaat gcacccggct gaacaaaagt gcgataaatg acatagccgt tctgttcgat
     5821 actcacttca gcgttgcttt tggcgatgcc gcgcactacc ggagcaaagc cacgctggct
     5881 gtccggcaac atttcatcgt cggatgccag taacgcccca cgcatttgta cgctatcaaa
     5941 aatctcgcca ggcgtggcgg tatcacccag agttatctgt gatttaagtg gaataattgc
     6001 ccggtttaac gatgtaccaa cgttatccca ttggtttttg ccatcggtac ggttccagat
     6061 actgttatta cgtaaacgcc acggaccaac gttcaaaccg ctgcgcaggt tcaggtaata
     6121 actgtcactg gtcgaatctg aagatgaacc tgtaccttca ttgcgtccat tgctgccact
     6181 aaagctgtaa tccagcaaca gcgcaggtat tccttcgtcc cagcgagaag gatcaaccgt
     6241 gccgcgagcg gtcatcatca ttgccgcctg tggaaaggaa agatgcagcg tctgagtatt
     6301 aaaatcaaag cggctggtgg cctggggaat gatttcatca aacgctacgc aggcttctgg
     6361 tggtgacatc ttcagcgccg gaaagctgtc aacgcgcacg cccatttctt ccagttgcac
     6421 ccggctaagg caaggcataa gcgactcacc gctggctttg cgtttcgctt cgctggcggt
     6481 actaaaatcc agcgtccggg aagccatttt ttcttcgttt acaaacaccg aaacccggta
     6541 tttaccgggg atttgctggc cgacaggtga gtcgtatacc gacaagtcga tgtgttgatc
     6601 gataccctga acgttttcga gaaatttagg gttaaatttt ccgccatacg caggtaacga
     6661 gctggcaatc gttaatgcca gcagagagaa ggaaaaatgc gctctgtagt gatagagagg
     6721 taaatgcatc caatgcatag taaaagcgcc ttgtaaaagg atcagttaat cggcttcgtc
     6781 agcatttcgc cagcaacacc gaaatcatta ataatgctgt atttcacttc gtgcgcgttg
     6841 cctgtaccgg ctggaaggtt aatgtttaac tttcctttcg cgagaaccat tcccgcccgt
     6901 tcaatctttt gtccattaat ggaaaactga ttaaaggtca ggttataagc ggagtcgtta
     6961 ttaaccacaa tttggttatt ttgacgctgg aaacgtaact gtttcatggc ctcgaggttg
     7021 cttcccggca aaccttttgg acgatagaac aacttcaaac gggtgcgaac ggctatctgt
     7081 aagacgtttt tattttcatt ttcagcactt ttcgcaggaa tggcctttac attaatccag
     7141 taaacggatt cacgatcttt aggtaaggta tcttcaatat tgacgatacg taagacgtta
     7201 tttttatcag gatcaagttt gaataatggc ggtgtaataa taaacggtgt ttttttattg
     7261 ccattcacat catcaatcca tgactggatc aaaaactctt catttttatt atggttttca
     7321 acggtcagcg acgcttcttt cttgccgcca tcataaataa cacgcgtcgt gcttaaggct
     7381 acgccaccag caaaacactg ttgtgcggtc attaaaagtg ccagcacagc aaagctaata
     7441 cgtttcatga ttcggtccct gagtcattgg tgtgaataat ggcaacacag tctctgttgc
     7501 cattgagaga tgatcgttcc gtcaggatcg attagttgta agtcaggttg aagtcgactt
     7561 cagcgttacc gtaacccgtg gttactgcat ctttggtaga gacatagtta gcggtgaaat
     7621 acagaacggt ctgaccttct ttcaggacag tagcagattt accagtattc aggtcaacca
     7681 gggtattggt gttatcataa ataccgatac ctacgccagt tgcggcacct ggttcagtgc
     7741 ttacagaaac cagtttgctg ttgtctgcat cgccaacgcc gttaaagctg atttccacat
     7801 ttttgaaagt ggagatatcg cagttttcca gtttgatttt gaacggcgtt gctgtggatt
     7861 tatcacccat agcagtaaat acagacttgt tcacctgacc cagaacaact tcttgttttt
     7921 gagtgtctgg agacacaaca caagcggcgt caacaatctc accggtgaat ttaacagtac
     7981 cgtcgcccgc atcagctgcg aatacggaag tagaggtcag cgcaagcgca gataaggcaa
     8041 acagaacctt tttcatttat aactccattt atatagataa a
//
LOCUS       DAPPNJ010000194         8025 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000194 DAPPNJ010000000
VERSION     DAPPNJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.196"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..891
                     /gene="potE"
                     /locus_tag="RPM73_003720"
     CDS             <1..891
                     /gene="potE"
                     /locus_tag="RPM73_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDX6522030.1"
                     /translation="RITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYVDSWNPHHAP
                     FFSAVGSSIAMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAVIYIVSTNVI
                     AGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSS
                     SDEGYFPKIFSRVTKVDAPVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNI
                     IPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTF
                     LGWTLYGLVSPRFELKNKHG"
     gene            complement(947..2587)
                     /gene="pgm"
                     /locus_tag="RPM73_003721"
     CDS             complement(947..2587)
                     /gene="pgm"
                     /locus_tag="RPM73_003721"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDX6522031.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(2613..3158)
                     /gene="seqA"
                     /locus_tag="RPM73_003722"
     CDS             complement(2613..3158)
                     /gene="seqA"
                     /locus_tag="RPM73_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDX6522032.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3343..4107
                     /gene="ybfF"
                     /locus_tag="RPM73_003723"
     CDS             3343..4107
                     /gene="ybfF"
                     /locus_tag="RPM73_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX6522033.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4247..4540
                     /gene="ybfE"
                     /locus_tag="RPM73_003724"
     CDS             4247..4540
                     /gene="ybfE"
                     /locus_tag="RPM73_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDX6522034.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            4680..5210
                     /gene="fldA"
                     /locus_tag="RPM73_003725"
     CDS             4680..5210
                     /gene="fldA"
                     /locus_tag="RPM73_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDX6522035.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5420..5506
                     /gene="uof"
                     /locus_tag="RPM73_003726"
     CDS             5420..5506
                     /gene="uof"
                     /locus_tag="RPM73_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDX6522036.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5499..5945
                     /gene="fur"
                     /locus_tag="RPM73_003727"
     CDS             5499..5945
                     /gene="fur"
                     /locus_tag="RPM73_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDX6522037.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(6029..6355)
                     /locus_tag="RPM73_003728"
     CDS             complement(6029..6355)
                     /locus_tag="RPM73_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522038.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6405..7811)
                     /gene="chiP"
                     /locus_tag="RPM73_003729"
     CDS             complement(6405..7811)
                     /gene="chiP"
                     /locus_tag="RPM73_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDX6522039.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1922 a         2034 c         2012 g         2057 t
ORIGIN      
        1 cgcattaccg ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc
       61 ctgtgcatta tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg
      121 catcatgcac cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt
      181 ctcggtctgg agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg
      241 ccaatcgcgg tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac
      301 gtgattgcag ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg
      361 gccttcgcac agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg
      421 tcctgctgcg gttcgctgct tggctggcag ttcaccattg cccaggtgtt taaatcttca
      481 tctgatgaag gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg
      541 cagggaatgt tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg
      601 tcgctaaaca gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg
      661 tatattctgt caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca
      721 aaagcgaaag ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg
      781 tactcatccg gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca
      841 ctgtatggtc tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa
      901 gtaaaaaagg gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca
      961 gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga
     1021 ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc
     1081 agccgttgtc agtcatcact ttcagaccgc caatagaagc accgttgccc ggagcagcag
     1141 tcaggcgcgc ggtgatcggg tcacctgcca gggtgctggc gctcaccatt tccggagaca
     1201 gcttagacag cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg
     1261 gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag
     1321 cggtgatttc cgccgccagc agacacataa tgatgccgtc tttgtcggtg gaccacggcg
     1381 tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc
     1441 cgtcgaacag accatcaaca aaccatttga aacctaccgg tacttctacc agcttacggc
     1501 ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa
     1561 catctttgcc ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt
     1621 ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt
     1681 tagcaaacgc cagatcgaac ttatcacgca gtgccagcag acccgccatc gcacactcgg
     1741 aggagcagtc catacggatc gcaccgtctt tatcgaggtg cataaagcgg aaggtttgat
     1801 cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaatg cgcttccagt
     1861 attcgatacc ggaaccgccc aacggatcaa cgcccagcgt caggcccgct ttctgaatcg
     1921 ccgccatatc aacgatatcg gccagccctt ccacgaacgg ctgcaccaga tcctgctctt
     1981 tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat
     2041 cggccatcag tgcgttggcc ctgtcttcca ccactttggt gacgttagta tcagccgggc
     2101 caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa
     2161 tcacgatacc gtctgccagc gggccacctt ttttattgtg aaccaggatg gcattggaaa
     2221 cggcaggcgt cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg
     2281 ccagcacttc cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca
     2341 catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg
     2401 ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac
     2461 cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg
     2521 tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga
     2581 ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc
     2641 aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg
     2701 gtgttggtgt tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga
     2761 ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc
     2821 aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc
     2881 agcataaagc gattgaccgc tcgcttttgc tctgcgtatt cgtccgaaag cagaagttca
     2941 cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt
     3001 gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc
     3061 aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata
     3121 tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg cagtatctta
     3181 gctgaataca agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct
     3241 ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa
     3301 cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc
     3361 gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt
     3421 ggtagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc
     3481 caggttgata tgcgtaacca cggtctttca ccgagagagc cggtaatgaa ttacccggcg
     3541 atggcgcagg atcttgttga taccctggat gcactgcaga tcgacaaagc aacatttatc
     3601 ggtcactcca tgggcggtaa agcggtaatg gcacttactg cactggcccc cgatcgcatc
     3661 gataaactgg tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag
     3721 attttcgcag ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca
     3781 gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt
     3841 gttgacgggg agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg
     3901 ggttgggaga aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct
     3961 ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg
     4021 catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc
     4081 gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc
     4141 tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat
     4201 ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca
     4261 aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac
     4321 taatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga
     4381 caaagtacgt ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct
     4441 gaacgagctg gcggaatcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat
     4501 gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccagtt tcatgtagca
     4561 cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc
     4621 tttatccgtg ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca
     4681 tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa
     4741 tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca
     4801 aagaagatct ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg
     4861 aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat ttcaatggca
     4921 aactggttgc gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgatg
     4981 cattgggcac catccgcgac atcattgaac cgcgcggtgc aaccatcgtt ggtcactggc
     5041 caactgcggg ctatcatttc gaagcatcaa aaggtctggc agatgacgac cactttgtcg
     5101 gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg
     5161 ttaaacagat ttctgaagag ttgcatctcg acgaaattct taatgcctga tgtgatgcgg
     5221 cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat
     5281 ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt
     5341 aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt
     5401 taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc
     5461 taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc
     5521 taaagaaagc tggcctgaaa gtaacgcttc cacgtttaaa aatcctggaa gttcttcagg
     5581 agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg
     5641 aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg
     5701 tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc
     5761 acgatcacct gatttgcctc gactgcggca aggttatcga atttagtgat gattccatcg
     5821 aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct
     5881 atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga tgagcacgcg cacgaaggca
     5941 aataagccag cctgaacgag aaaagccaac ctgcgggttg gcttttttat acaagggaaa
     6001 taagaaaagc gagagttaca gctctcactt atttgttatt actgcgtatt tcctgccaga
     6061 ccttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca cgcagacact
     6121 gctgataatc cagtacgcgt acactttcct ggtcggcaaa ctgctgatgt tgtttctctt
     6181 tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttctggcgaa ccttgtgcgg
     6241 tgttgataca ggcgctgtat gcctctttca gacgactgtc ttcctgtggc gcggttgatt
     6301 gcgcacaagc taccagcccc gatgccatta tggcgatgag aatcagtttt ttcatagcaa
     6361 attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat
     6421 ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttacc gtaaccgccg
     6481 ccccagcttg ggatgtcaga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg
     6541 cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtaggc agactcttca
     6601 atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc aggtttagca
     6661 tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcagg
     6721 tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc ggaacggtta
     6781 tcccaccaga tatccaggcg gccgttggag gaagcgtagg ttggagtcat acgttgcagg
     6841 aagtatccct gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa atccactacg
     6901 tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc ataaaggtcg
     6961 ttgacgctgc gatcgtcaac tttatcgcgc gtaccataga actggtagct ggtggttaac
     7021 gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc gatataccct
     7081 tccgcctgac caaatgccgc ttccagtacg aagttatttt tgaagtcgta tttcgcccca
     7141 agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc
     7201 agatgccacg gcgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca
     7261 tcgccgtaat caaaattcgc cccggcttcc gccccctgat aagtacctgg cataaagctc
     7321 cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct cgcccaaacc
     7381 ggaccgtatt taaatttggc cgcagcttta tacaggctta taccgctttt gtcgccggac
     7441 cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgggagctg
     7501 tcgccgtttt ccgccatttc aatcgccgta aacgcagcaa tatcaaggcc gaacatatca
     7561 gcagcataac cggactgaaa atcgaggttg gcattccagg tggagtgaga aaggttggtt
     7621 ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatatata
     7681 ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg cattgccata
     7741 aagcccgaca ttgctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta
     7801 aacgtacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg
     7861 aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact
     7921 atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa
     7981 ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcgg
//
LOCUS       DAPPNJ010000195         8023 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000195 DAPPNJ010000000
VERSION     DAPPNJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.197"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            215..1066
                     /locus_tag="RPM73_003730"
     CDS             215..1066
                     /locus_tag="RPM73_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDX6522040.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            1059..1430
                     /locus_tag="RPM73_003731"
     CDS             1059..1430
                     /locus_tag="RPM73_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDX6522041.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1427..2179
                     /gene="fliP"
                     /locus_tag="RPM73_003732"
     CDS             1427..2179
                     /gene="fliP"
                     /locus_tag="RPM73_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDX6522042.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2182..2454
                     /gene="fliQ"
                     /locus_tag="RPM73_003733"
     CDS             2182..2454
                     /gene="fliQ"
                     /locus_tag="RPM73_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HDX6522043.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2456..3238
                     /gene="fliR"
                     /locus_tag="RPM73_003734"
     CDS             2456..3238
                     /gene="fliR"
                     /locus_tag="RPM73_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HDX6522044.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3228..4367
                     /gene="flhB"
                     /locus_tag="RPM73_003735"
     CDS             3228..4367
                     /gene="flhB"
                     /locus_tag="RPM73_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDX6522045.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWHEGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4351..6444
                     /gene="flhA"
                     /locus_tag="RPM73_003736"
     CDS             4351..6444
                     /gene="flhA"
                     /locus_tag="RPM73_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDX6522046.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITYPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6549..6827
                     /locus_tag="RPM73_003737"
     CDS             6549..6827
                     /locus_tag="RPM73_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDX6522047.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6820..7176
                     /locus_tag="RPM73_003738"
     CDS             6820..7176
                     /locus_tag="RPM73_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDX6522048.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(7233..8006)
                     /locus_tag="RPM73_003739"
     CDS             complement(7233..8006)
                     /locus_tag="RPM73_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDX6522049.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         1805 a         2093 c         2213 g         1912 t
ORIGIN      
        1 gcctgatgcg atgcggcgcg tcttatcagg cctacggttc gtgcaatgtg ttatgacgat
       61 gcctgatatc tcgcccttta ataatttaat tattttttca ccctattaat aatttgcccc
      121 tttcacgccc cctctattta atacctcacc ttgtgtcact gccggatttg cgtgcacaaa
      181 atcgcccata ccattacagc agagaaagta agccatgctg aagtacagta aaacgccagg
      241 catctttaaa ttagaaggta atcggctcgg acgcccctac catcgcctgc cgaccctgtt
      301 tacagggaat tttgacgtga ttgattcgca tctgggcagc tactttctca aaaagcatcg
      361 cagcaatatc accctaaaaa agattggctg cgaaatggat attattaata aaaacgctga
      421 actgatggtt tcccaggtcg ggcatctggc gtttgatatt gatcgctctt tgctgttaat
      481 gctgctgggc aatttttatg gtctggagtc ctctcttgaa gaggcgaaag cgcataacgg
      541 gctgccgacc aaaaccgaaa cgcggttaaa aaaccgtctg gcgctggata tctgcacgct
      601 gatttttaat ctgcaaacct ccggtattgc gctgaaatta aagctggaca gcagcacggt
      661 aattacccac tgggcgtatc aattaacctt tacccttgcg ggcgatgaag agagctgttt
      721 tcgtattttg ctcgacgacg cccacaccga ttttatttta aatctgatcc gccacagcga
      781 gcacagtaaa ccgcagcagg tggcgaaatc ggtcaataaa ccggccctga ttaaagagat
      841 catccgctcc ctgccgctga ccctgaacgt caaaatcgcc gagctatcga tgaatgtcgc
      901 ggatctcacg cagataaaag ccggggacat tttacccatc tcgctgggag aaacgttccc
      961 ggtggcgatt gggcaatctg aattatttag cgccctgatt gtggaagaca aagacaaact
     1021 gtttttgtcg gagctggcgg gcaaaaatga gaatagccat gagtaagcaa gaagatattt
     1081 tagccgggga ttttggcctc accgatgaag ttgcgcctgt cgcaaaatcc gccgacgctg
     1141 aaacgctggt cacccgcctg gaagaccgtt tttccgactc gatgacgctg ctaaaacgca
     1201 ttccggtgac cttaacgctg gaagtgtcat cagtagaaat aatgttagcc gacctcctta
     1261 acatcgacga cgacacggtg attgagctgg ataaactcgc cggggagccg ctggatatca
     1321 aagtgaacaa tatcctgctg ggcaaagcgg aagtggtggt ggtcaacgag aagtacggcc
     1381 tgcgcgtgct ggaatttaac acccgcgata tcaacgacct ggcaccatga aacgccgcac
     1441 tcaactgacg ctgggccttg ggctgctggc gcttgcgccg gtggcgttgg cccagggcgg
     1501 cgatattgcg ctgcttaacg tggtcaccca cggcaacact caggagtaca gcgtcaaaat
     1561 ccaggtgctg attctgatga ccctcgtcgg tttgctgcct acgatggtgc tgatgatgac
     1621 ctgctttacg cgctttatta tcgtgctgtc gctgctgcgc caggcgctag ggctacaaca
     1681 aacgccgcca aaccgcattc tgattggcat tgcgctctcc ctcaccatgc tggtgatgcg
     1741 ccccatctgg ctcaatattt acgaccacgc agtagtgccg tttgaaaatg atcagattac
     1801 cctgaccgat gcgttaagca ccgccgcgac gccactcaaa cgctttatgc tggcgcagac
     1861 tgacaaaaaa gcgatggcgc aaatcatgac tatcggcggc gcgaagggcc atgccgccga
     1921 ccaggatctc tcgattgtgg tgcccgccta tgtgttgagc gagctgaaaa ccgccttcca
     1981 gattggcttt atgatttaca tcccgtttct ggttatcgac ctgattgtcg ccagcgtact
     2041 gatggcgatg gggatgatga tgctgtcgcc gctgatcgtt tcgctaccct ttaagctgat
     2101 gctgtttgtg ctgatcgacg gctggtcgct gaccatcggt acactcacca ccagcattcg
     2161 cgggctgggg ctgggctgat gatgctgacc atagacgtag ccgccgacat tgtcgccagc
     2221 ggcataaaag tggtgatttt gctggtttcg gtgctggtag tgccgagcct gctggtcggc
     2281 ctgctggtga gcgtgttcca ggcggtaacg cagattaacg aacagaccct aagctttttg
     2341 ccgaggctga ttgtcacgct ggtggtgctg ggcgtgtgcg gtaaatggat gattatccag
     2401 ttgcatgatc tctgcattca cctgtttacc caggccgcgt tgctggttca ttgagatgcg
     2461 cacaagcgac gttacgcaac tgatggatct ggcgctgggg ctgtggtttc cctttgtgcg
     2521 cattatggcg tttctgcgct atgtgcctgt gctggataac agcgccctga ccgtgcgcgt
     2581 gcggattatt ttgtcgctgg cgctggcaat catcatcacg ccgttgatcc cccatcctgt
     2641 tccccacgac ctgatatcgc tcaacagcct gatcctgacg gtggagcaaa ttttatgggg
     2701 catgttattc gggctgatgt ttcagttttt gtttctggcg ctacagctgg ccgggcaaat
     2761 tctctcgttc aatatgggga tgagcatggc ggtgatgaac gacccgagca gtggcgcatc
     2821 gaccaccgtg ctggcggagc tcatcaacgt ctacgcgatc ctgctgtttt ttgccatgga
     2881 cgggcatctg ctgctggtga gtgtgctgta taagggcttt acctactggc cgattggcaa
     2941 cgcgctgcat ccgcaaaccc tgcgcaccat cgcgctggcc tttagctggg tgctggcgtc
     3001 cgcatcgctg ctggcgctgc caaccacttt tatcatgctg attgtgcagg gctgctttgg
     3061 cctgctcaac cgcattgcgc cgccgctgaa ccttttttca ctcggttttc ccatcaacat
     3121 gctggcgggg ctggtctgct ttgccaccct gctctacaac ctgccggatc actatctgca
     3181 tctggcgaat tttgtgttgc agcaactcga cgcgctgaaa ggtcactatg gcggatagca
     3241 gcagcgaaga aaaaacagag aaaccctcgg cgcaaaagct gcgtaaagcg cgcgaagagg
     3301 ggcaactgcc gcgctcgaag gatatggggc tggcggcgag cctgtttgcg gcatttgtgg
     3361 tgatctccag cagcttcccg tggtatgccg attttgtgcg cgagagtttt atcagcgtgc
     3421 accagtatgc acaggagatt aataacccag aggtgatcgg ccagtttctg cgccatcacc
     3481 tgttaattct cggcaagttt attctcacct tgctgccgat gcccgccgcc gcgctactct
     3541 cctcactagt gcccggcggc tggctgtttc tgccgaaaaa aatcctcccg gatttcagca
     3601 aaatcagccc gttaaaaggc attgggcggc tgttttccag tgaacatctg gcggaaacgg
     3661 gaaagatgac ggtgaagtcg gtggtggtgc tggtgatgct gtgggtaagc ctgcgcaata
     3721 actttgcctc ctttctcggc ctgcaggcgc taccgttcaa actggcgatg aatgagggtt
     3781 tatcgctcta cgcctgcgtg atgcgcaact ttgtcatcct gtttatcttc tttgcgttaa
     3841 tcgacgtgcc gctggcgaaa gccctgttta ccaaagggct gaagatgacc aaacaggagc
     3901 tgaaagaaga gtataaaaac caggaaggca agccggaagt gaaggcgcgc gtgcggcgtc
     3961 tgcaacggca gttggcgatg gggcaaattc gcaaagtggt gccgaaagcc aacgtggtga
     4021 tcaccaaccc gacccactac gccgtggcgt tgcagtatga ctcgtcgcgc gccgccgcgc
     4081 cgtttgtggt ggcaaaaggc accgatgaaa tcgccctcta tattcgccag gtcgccgctg
     4141 agaatcaggt ggaagtggtc gagttcccgc gactggcgcg ctcggtttac tacaccaccc
     4201 aggtgaacca gcaaattccg tttcagcttt accgggccat cgcccacgtt ctgacctacg
     4261 tcctgcaaat gaaacactgg cacgagggtg cacagccgcg cccggccctg aacagacata
     4321 tttccattcc caaagaggtg cttaaactgg atggcgaaaa caactaaatc gttcctcgcg
     4381 ctgctgcgcg gcggcaatct tggcgtgccg ctggtgatcc tgtgtattct ggcgatggtt
     4441 attttgccgc tgccgcctgc gctgctggat attctgttca ccttcaacat tgtgctggcg
     4501 gtgatggtgc tgctggtggc ggtgtcggcg aaacgaccgc tggaattcag cctgttcccg
     4561 accattttgc tgatcaccac cttaatgcgc ctgacgctca acgtcgcctc tacgcgcgtg
     4621 gtgctgctgc acgggcatct cggcgcggac gcggcgggta aggtgattga gtcgtttggt
     4681 caggtggtga tcggcggcaa ctttgttgtc ggctttgtgg tgtttatcat cctgatgatc
     4741 atcaacttta ttgtcgtcac caaaggggcc gagcgtattt ccgaggtttc agcccgcttt
     4801 accctggatg cgatgcccgg taagcagatg gcgattgacg ccgatcttaa tgccgggttg
     4861 atcaatcagg cgcaggcaca aacccgacgt aaagatgttg ccagcgaggc cgatttctac
     4921 ggcgcgatgg acggggcatc gaaatttgtg cgcggggatg ctatcgccgg gatgatgatt
     4981 ctggcgatca acctgatcgg cggcgtctgt atcgggatct tcaaatacaa cctgagcgcc
     5041 gacgccgcct tccagcagta tgtgctgatg accatcggcg acgggctggt ggcgcaaatc
     5101 ccttccctgc tactctccac cgcggcggcg attatcgtca cccgcgtcag cgacaacggc
     5161 gatatcgccc atgacgtgcg tcaccagttg ctggcaagcc catcggtgct ctacaccgct
     5221 accggaatta tgttcgtact ggcggtagtg cctggaatgc cacatctgcc gtttttgctg
     5281 ttcagcgccc tgcttggctt taccggctgg cggatgagca aacgcccgca ggcggcggaa
     5341 gcggaagaga aaagtctcga aacgctgacc cgcaccatca ccgaaaccag cgagcaacag
     5401 gtcagttggg aaaccattcc gctgatcgag cccatcagct taagcctcgg ctacaagctg
     5461 gtggcgctgg tggacaaagc ccagggcaac ccgctcaccc agcgtattcg cggcgtgcga
     5521 caggtgatat ccgacggcaa cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc
     5581 cgccttaagc ccagccagta cgctattttc atcaacggca ttaaggctga tgaagcggat
     5641 attccggcgg ataaactgat ggcgctgccc tccagcgaaa cctacggcga gatagacggc
     5701 gtgctgggga acgacccggc gtacgggatg ccggtgacct ggatccagcc tgcgcagaaa
     5761 gcgaaggcgc tgaatatggg gtatcaggtg atcgacagcg ccagcgtgat tgctacccat
     5821 gtaaataaga ttgtgcgcag ctatattcct gatttattta actatgacga tattacgcag
     5881 ttgcataacc gtttggcgtc gatggcaccg cgcctggcgg aggatttaag cgcggtgctc
     5941 aattacagcc agttgctgaa agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc
     6001 gatatcgtca ccatcgccac cgtgctggtc gccagtagcg cggtgaccaa agatcatatt
     6061 ctgctggcag ccgatgtgcg cctggcgctg cggcgcagca ttacctatcc gttcgttcgc
     6121 aagcaggagc tgacggtgta taccctgaat aatgagctgg aaaatctgct gactaaccta
     6181 gtgaatcagg cgcaacaggg cggtaaagtg atgctcgaca gcgtgccagt ggacccgaat
     6241 atgctcaacc agttccagag tacgatgcca caggtgaaag agcagatgaa agcggcgggg
     6301 aaagacccgg tgctgctggt gccaccgcag ctgcgccctt tgctggcgcg ttatgcaagg
     6361 ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag tgccggatga gctggagttg
     6421 aagattatgg gggcgttgag ttgatggggg attcttttcc atgctttgca gcacctgaca
     6481 ccattaattg acgtcacact gcaaagagac tacgatgaca ccatacaatg acgtcatgga
     6541 ttgatagtat gattaaggtc agcgcgctag gttctcgtca tagaaaaaca ttcgacgatg
     6601 taatgacgga gccgccaaaa tctggaatta aatgggatga tgttgttgca ctcattaaag
     6661 ctgtgggtgg aacaataaaa aataacaacg gctccagacg aaaatttcag attggaacga
     6721 ccaaattcag cacacatgag ccacatccaa aaaatgtgat ggataaaggc gcagtagcag
     6781 gtcttaagga gtggtttgtt aactgtgtag gggtagatta tgaataaagc atcgaacatt
     6841 atcacaatta aaggtcaacc agccgcagta acatttgaag ccgatatcaa cgcatttcgc
     6901 ggcaagttcc tcaatgtaaa cggttattgt gactttgtag ccacaagtat tgaggcatta
     6961 tatcgtgaag gcgaaagcgc gctggatgag tggatggcgg attgtgaaga ggacggtatt
     7021 gccccttttc gtgaagaaga agagcaaaaa cgcctgacgc ttcgtgttcc tcatcacatt
     7081 gattcgcggc tgaccatcgt cgccagacaa cattccattt caaaaaacca gctcattgtt
     7141 gatgtcttag aacgtgagtt ttcagcccag atgtaaacag gtcggatgcc gcgtggcatc
     7201 cgaccttttg ccagttcgtc aatattaccc atttacaaaa tcgtctcctc cgtcaaaatc
     7261 cgccgcgcgc ctaaaaagtg atcctgccag aaaggttcag ctaactgact tacccgaatg
     7321 gtttccccgg tgcgcggtga ctcaataaac tgaccgtcgc ccagatacac ccccatatgg
     7381 tcggcaatct cgcggctgtg gatatggaaa aatagcaaat ctccccggcg caggtcgtta
     7441 ttcgccacaa tggttgcccg gcgatagtga tacatctcat tagccgtgcg tgggagtttc
     7501 gcctcaagga tcttgttgta ggcataaaaa actaacccgc tacagtcgaa gccttgatcg
     7561 ggccgcgtac cgccccagac gtaaggcttg ccaagctgtt gctccagacg gtgaatcgcc
     7621 acttcggtga tgttatgcag atggtcgctg cttaagaaat ggttgttttc cgccagcgcc
     7681 tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc attctggatg ctgttttatc
     7741 tgttcgcggt tgtgctggcg tagcgccctt ctgctttcgg cattgccttc cacaatatag
     7801 ctggcctgtt ttttcagatg ggtttggtac tgttttaata aacgcgcacg attttgcatc
     7861 cgctggcggg cggcgtagct gaaagtgata tgtgatgtgg tggcgctggc aaatgaagaa
     7921 aacggcaagc aaagcaacag taatcccgac aacacaaaag agggaatcga cggcaaggac
     7981 atactcaata atggctgatc atgcattgat gttgcagtgt atc
//
LOCUS       DAPPNJ010000196         8013 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000196 DAPPNJ010000000
VERSION     DAPPNJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.198"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            59..520
                     /gene="hycA"
                     /locus_tag="RPM73_003740"
     CDS             59..520
                     /gene="hycA"
                     /locus_tag="RPM73_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDX6522050.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            645..1256
                     /gene="hycB"
                     /locus_tag="RPM73_003741"
     CDS             645..1256
                     /gene="hycB"
                     /locus_tag="RPM73_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDX6522051.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            1253..3079
                     /gene="hycC"
                     /locus_tag="RPM73_003742"
     CDS             1253..3079
                     /gene="hycC"
                     /locus_tag="RPM73_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDX6522052.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            3082..4005
                     /gene="hycD"
                     /locus_tag="RPM73_003743"
     CDS             3082..4005
                     /gene="hycD"
                     /locus_tag="RPM73_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDX6522053.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVIGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            4023..5732
                     /gene="hycE"
                     /locus_tag="RPM73_003744"
     CDS             4023..5732
                     /gene="hycE"
                     /locus_tag="RPM73_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDX6522054.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            5742..6284
                     /gene="hycF"
                     /locus_tag="RPM73_003745"
     CDS             5742..6284
                     /gene="hycF"
                     /locus_tag="RPM73_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDX6522055.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            6284..7051
                     /gene="hycG"
                     /locus_tag="RPM73_003746"
     CDS             6284..7051
                     /gene="hycG"
                     /locus_tag="RPM73_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDX6522056.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            7048..7458
                     /gene="hycH"
                     /locus_tag="RPM73_003747"
     CDS             7048..7458
                     /gene="hycH"
                     /locus_tag="RPM73_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDX6522057.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            7451..7921
                     /gene="hycI"
                     /locus_tag="RPM73_003748"
     CDS             7451..7921
                     /gene="hycI"
                     /locus_tag="RPM73_003748"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDX6522058.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
BASE COUNT         1646 a         2135 c         2362 g         1870 t
ORIGIN      
        1 aaaagttggc acaaaaaatg cttaaagctg gcatctctgt taaacgggta acctgacaat
       61 gactatttgg gaaataagcg agaaagccga ttacatcgca cagcggcatc gtcgcctaca
      121 ggaccagtgg cacatctact gcaattcgct ggttcagggg atcacgttat cgaaagcgcg
      181 cctgcatcac gccatgagct gcgcgccgga caaagaactc tgtttcgtcc tttttgaaca
      241 ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc cacaccatcg agtattacgt
      301 cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag gcgcaactga gcattgacgg
      361 catgattgat ggcaaggtca acatccgcga tcgcgaacag gttctggaac actatctcga
      421 aaaaatcgct ggcgtttacg acagcttata caccgccatt gaaaacaatg tgccggtgaa
      481 tttaagccaa ctggtaaagg gacaaagccc ggcagcatga gctgaggctt tgcccgtttt
      541 gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag tgtcgaaaat gacatttcat
      601 cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg cctggtgaat cgttttgtaa
      661 ttgctgactc cacgctctgt atcggctgcc acacttgtga ggccgcctgt tcggagacgc
      721 atcgccagca cggcctgcaa tcaatgccgc gcctgagagt gatgctgaat gaaaaagaat
      781 ctgcgccgca gctctgtcac cactgtgaag atgcaccctg cgcggtggtc tgcccggtta
      841 acgccatcac ccgcgttgat ggggccgtgc agttgaatga aagcctgtgc gtaagctgca
      901 agctgtgcgg catcgcctgc ccgtttggcg caattgaatt ttccggcagc cgtccgctgg
      961 atattcctgc aaatgccaat accccgaaag cgccaccggc accgcctgct ccggcgcgta
     1021 tcagtacgct gcttgactgg gtgccgggta ttcgcgcgat cgccgtcaaa tgtgaccttt
     1081 gcagttttga tgaacaaggt ccggcctgcg tgcggatgtg cccgactaaa gccctgcatc
     1141 tggtggataa caccgatatc gcccgcgtca gcaaacgtaa gcgtgagctg acctttaaca
     1201 cggactttgg cgatctcacc ttgtttcagc aggctcaaag tggagaggct aaatgagcgc
     1261 aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc gccgctgttc tggcatttct
     1321 cttttctttt caaaaagcgc taagtggctg gatagccgga attggcggcg cggttggtag
     1381 tctgtatacg gcagccgcgg gcttcactgt actgactggc gcggttggcg tgagcggtgc
     1441 gctgtcgctg gtaagctacg atgtgcaaat ctccccgctt aacgcgattt ggctgattac
     1501 cctcggcctt tgcggtctgt ttgtcagcct ctataacatt gactggcatc gccacgcgca
     1561 ggtgaagtgc aacggcttgc agatcaatat gttgatggct gccgccgtct gcgccgtcat
     1621 cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc atggccttgt gtgcggtgtt
     1681 cctcaccagc aacagcaaag agggcaaact gtggtttgcg ctggggcgtc ttggcactct
     1741 gctgctggtg attgcttgct ggctgctgtg gcagcgttat ggcacgctgg atctgcgcct
     1801 actggatatg cgtatgcaac agctgccgct cggttccgat atctggctgc tcggtgtgat
     1861 tggctttggc ctgctggccg ggattattcc gctgcacggc tgggtgccgc aggcacatgc
     1921 taacgcctct gcgccagctg ccgcgttgtt ttctacggta gtcatgaaaa ttggcctgct
     1981 gggcatgtta accctgtcac tgcttggcgg taatgcaccg ctgtggtggg ggatcgcgct
     2041 gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg tatgcgttga tggaacacaa
     2101 catccagcgc ctgctggctt accacaccct ggaaaatatc ggcattatcc tgctggggct
     2161 gggcgctggc gtaacgggta tcgcgctcga acaaccggca ctgattgctc ttggcctggt
     2221 cggtggtctg taccatcttc ttaaccatag cctgttcaaa agcgtactgt tcctcggggc
     2281 ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa aaactcggtg gtattggcaa
     2341 gaaaatgccg gttatctcca tcgccatgtt agtcgggttg atggcaatgg ctgcgctgcc
     2401 gccactgaac gggtttgccg gggaatgggt tatctatcaa tccttcttca aactgagcaa
     2461 tagcggcgcg tttgttgccc gtctgctggg gccgctgctc gctgtgggtc tggcaattac
     2521 cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc gtcacgttcc tcggcgcgcc
     2581 gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc ctgatgagcg taagcgtagt
     2641 ggcactggcg atttgctgcg taattggcgg tgttgctgcg ccgtggctgc tgccgatgct
     2701 ctctgctgct gtacctctgc cgctggagcc tgctaacacc accgtttctc aaccgatgat
     2761 cacgttgctg ctgattgcct gcccgctgct gccattcatc attatggcga tttgcaaagg
     2821 cgatcgtttg ccgtcgcgtt cccgcggtgc ggcctgggtg tgcggctacg accacgaaaa
     2881 atcaatggtg attaccgctc acggttttgc catgccggtg aaacaggcgt ttgcgccggt
     2941 gctgaaacta cgcaaatggc tgaatccggt gtctctggtg ccgggctggc agtgcgaggg
     3001 gagtgcgttg ctgttccgcc ggatggcgct ggttgaactg gcggtgctgg tggtgattat
     3061 tgtttcacga ggagcctgag aatgagtgtt ttatatccgt taattcaggc gctggtgcta
     3121 tttgccgttg cgccgctgct ctccggtatt acccgcgtgg cgcgagcccg cttacataac
     3181 cgtcgtgggc cgggtgtgtt gcaggagtat cgtgacatta tcaaactgct gggccgccag
     3241 agcgttggcc cggatgcctc cggctgggtg ttccgcctga cgccgtatgt gatggtgggc
     3301 gtcatgctga ctatcgctac tgcgctgccg gtggtgaccg ttggttctcc gctgccgcaa
     3361 ctgggcgatt tgatcacctt actgtatctc tttgccatcg cgcgtttctt ctttgctatt
     3421 tccggtctgg ataccggtag cccgtttact gctatcggcg cgagccgtga agcgatgctt
     3481 ggcgtgctgg tcgaaccgat gctgctgctt ggtctgtggg ttgccgcaca ggttgccggt
     3541 tccaccaaca tcagcaacat caccgacacc gtttatcact ggccgctgag ccagagcatc
     3601 ccgctggtgc tggcgctttg tgcctgtgcg ttcgccactt ttatcgaaat gggcaaactg
     3661 ccgttcgacc tggcggaagc cgagcaggag ttgcaggaag gtccgctctc cgaatacagc
     3721 ggtagcggct ttggcgtcat gaaatggggt atcagcctga aacagctggt ggtgttgcag
     3781 atgttcgtcg gggtgtttat tccgtgggga caaatggaaa ccttcaccgc cggtggactg
     3841 ctgctggcgc tggtgattgc catcgtcaaa ctggtgatcg gcgtcctggt tatcgcgctg
     3901 ttcgaaaaca gcatggcccg tctgcgtctt gatattactc cgcgcattac ctgggctggg
     3961 tttggctttg catttttagc gttcgtctcc ttgctggcgg cgtgattaaa gagagtttga
     4021 gcatgtctga agaaaaatta ggtcaacatt atctcgccgc gctgaatgag gcatttccgg
     4081 gcgtcgtgct ggaccacgcc tggcagacca aagatcagct gaccgtgacc gtgaaggtga
     4141 actacctgcc ggaagtggtg gagtttctct actacaagca gggtggctgg ctgtcggtgc
     4201 tgtttggtaa cgacgaacgc aaactgaatg gtcattacgc cgtttattac gtgctgtcga
     4261 tggagaaggg caccaagtgt tgggtaacgg ttcgcgtcga agttgacgcc aacaaaccgg
     4321 agtatccgtc cgtgacgccg cgcgttccgg cggcggtgtg gggcgagcgc gaagtgcgcg
     4381 atatgtacgg tttgattccg gttggtctgc cggatgaacg tcgtctggtg ctgccggatg
     4441 actggccgga tgaactttat ccgctgcgta aagacagcat ggattatcgt cagcgtccgg
     4501 caccgactac cgatgctgaa acctacgagt tcatcaacga actgggcgac aagaaaaaca
     4561 acgtcgtgcc gattggtccg ctgcacgtca cttctgacga accgggccac ttccgtctgt
     4621 tcgtcgatgg cgaaaacatt atcgacgccg actaccgtct gttctacgtt catcgcggca
     4681 tggaaaaact ggcggaaacc cgtatgggtt acaacgaagt gaccttcctc tctgaccgtg
     4741 tgtgcgggat ctgcggcttt gcccacagca ccgcctacac cacgtcggta gaaaacgcga
     4801 tgggtattca ggtgccagaa cgtgcgcaga tgatccgcgc cattctgctg gaggtggaac
     4861 gcctgcactc gcatctgctc aacctcggtc tcgcctgcca ctttaccggc tttgactccg
     4921 gctttatgca gttcttccgc gtgcgtgaaa cctccatgaa aatggcagag atcctcaccg
     4981 gtgcgcgtaa aacctacggc ctgaacctga tcggcgggat tcgtcgcgat ctgttgaaag
     5041 atgacatgat ccagacccgc cagctggcgc aacagatgcg tcgtgaagtg caggagctgg
     5101 tggatgtgct gttgagcaca ccgaacatgg aacagcgcac tgttggcatt ggtcgtcttg
     5161 acccggaaat cgcccgcgat ttcagtaacg ttggcccgat ggttcgcgcc agcggtcacg
     5221 cccgcgatac tcgcgccgat cacccgtttg tcggttatgg cctgctgcca atggaagtcc
     5281 acagcgagca gggctgcgac gttatttccc gtctgaaagt gcgtatcaac gaagtttata
     5341 ccgcgctgaa catgatcgac tacggtctgg ataacctgcc gggcggtccg ctgatggtgg
     5401 aaggctttac ctacattccg caccgtttcg cgctgggctt tgccgaagcg ccgcgcggcg
     5461 acgatatcca ctggagcatg accggcgaca accagaagct gtaccgctgg cgctgccgtg
     5521 ccgcgaccta cgcgaactgg ccgaccctgc gctacatgct gcgcggtaac accgtttccg
     5581 atgcgccgct gattatcggt agcctggacc cttgctactc ctgtaccgac cgcatgaccg
     5641 tggtcgatgt gcgtaagaaa aagagcaaag tggtgccgta caaagaactc gagcgttaca
     5701 gcattgagcg taaaaactcg ccgctgaaat aaggaatcgc catgtttacc tttatcaaaa
     5761 aagtcatcaa aaccggcacg gcgacctcgt cttatccgct ggagccgatt gcggttgata
     5821 aaaacttccg tggtaagcca gagcagaacc cgcagcagtg catcggctgc gcggcctgcg
     5881 tcaatgcctg cccgtcaaac gccttaacgg ttgaaactga cctcgccaca ggagagcttg
     5941 cgtgggagtt taatctcggg cgctgcatct tctgtggacg ctgcgaagaa gtctgcccga
     6001 cggcagcgat caaactgtcg caagagtacg aactggcggt gtggaagaaa gaagatttcc
     6061 tgcaacagtc ccgttttgtg ctgtgcagct gtcgcgtctg caatcgtcct ttcgccgtcc
     6121 agaaagagat cgactacgcc attgcgctgc ttaagcacaa tggtgacagc cgcgcggaaa
     6181 accaccgcga aagctttgag acttgcccgg aatgtaagcg ccagaaatgc ctggtgccgt
     6241 ccgaccgtat tgaactgact cgccatatga aagaggccat ctgatgagca atttattagg
     6301 cccccgtgac gccaacggca ttccggtccc gatgacggtg gatgaatcca tcgccagcat
     6361 gaaggcgtcg ttactgaaaa aaatcaaacg ttctgcctac gtttaccgcg tggactgcgg
     6421 cggctgcaac ggctgcgaaa tcgaaatttt cgccacgctt tcgccgctgt ttgatgcaga
     6481 acgcttcggc attaaagtcg ttccttcacc gcgtcatgcg gatattttac tgtttaccgg
     6541 cgcggtcacc cgtgcaatgc gatcccctgc gctgcgtgcg tggcagtccg cgccggaccc
     6601 gaaaatctgt atctcctacg gtgcctgcgg taacagtggc gggatcttcc acgatctcta
     6661 ctgcgtgtgg ggcggtacgg ataaaatcgt cccggtagat gtctacatcc ctggttgccc
     6721 gccaacgcct gccgccacgc tgtacggctt tgcaatggcg ctcggcctgc tggagcagaa
     6781 aattcacgcc cgtggcccgg gtgagcagga tgaacaaccg gcggaaatcc tgcatggcga
     6841 tatggtgcag ccgctgcgtg tgaaagtgga tcgcgaagca cgccgcctgg caggttatcg
     6901 ttacggtcgt cagattgccg atgacttcct gacacagtta gggcagggcg aagaacaggt
     6961 tgcacgctgg ctggaagcgg aaaacgatcc gcgtctgaac gagattgtca gccatctgaa
     7021 tcatgttgta gaagaggcgc gtatccgatg agtgaaaagg tggtgttcag tcaactgagc
     7081 cgtaaattta ttgatgagaa cgatgccacg cccgccgagg cgcagcaggt ggtctattac
     7141 agcctggcga ttggtcacca ccttggggtt atcgattgcc tggaagcggc gctcacctgc
     7201 ccgtgggatg aatatctggc atggattgcc actctggagg cgggcagcga agcccgccgc
     7261 aaaatggaag gcgtgccgaa atatggtgag atcgtcatcg acattaacca tgtgccgatg
     7321 ctggccaacg cattcgataa agcccgggca gcgcaaactt cgcaacagca ggaatggagt
     7381 acaatgctgc taagtatgct gcatgatatt catcaggaaa acgccatcta tttgatggtg
     7441 aggagactgc gtgactgacg ttttactctg tgttggcaat agcatgatgg gcgatgatgg
     7501 cgcaggcccg ctgctggcgg aaaagtgcgc cgccgcgccg aaaggtaact gggtggtgat
     7561 tgacggcggt agcgcaccgg aaaacgacat cgtcgccatc cgtgaactgc gccctacgcg
     7621 gttactgatt gtcgacgcca ctgatatggg gctaaacccc ggcgagatcc gcatcatcga
     7681 cccggatgat atcgccgaga tgtttatgat gactacccat aacatgccgt tgaactatct
     7741 tatcgaccag ttgaaagaag acattggcga agtgattttc ctcggcatcc agccggatat
     7801 cgtcggcttt tactacccga tgacccagcc gattaaagat gcggtagaaa ccgtttatca
     7861 acgactggaa ggctgggaag gaaatggcgg attcgcgcag ttagcggtgg aagaagagta
     7921 gtttttcaat aaggaatcag gacagggatg ttcttgatgg ggtgaaccag ctctgatgcc
     7981 aaatgctaaa ttgcccgatg cgctgcgctt atc
//
LOCUS       DAPPNJ010000197         8009 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000197 DAPPNJ010000000
VERSION     DAPPNJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.199"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(15..635)
                     /gene="sodA"
                     /locus_tag="RPM73_003749"
     CDS             complement(15..635)
                     /gene="sodA"
                     /locus_tag="RPM73_003749"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDX6522059.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            920..1954
                     /gene="rhaT"
                     /locus_tag="RPM73_003750"
     CDS             920..1954
                     /gene="rhaT"
                     /locus_tag="RPM73_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDX6522060.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(1951..2817)
                     /gene="rhaR"
                     /locus_tag="RPM73_003751"
     CDS             complement(1951..2817)
                     /gene="rhaR"
                     /locus_tag="RPM73_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDX6522061.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(2873..3709)
                     /gene="rhaS"
                     /locus_tag="RPM73_003752"
     CDS             complement(2873..3709)
                     /gene="rhaS"
                     /locus_tag="RPM73_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDX6522062.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRNHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            3997..5466
                     /gene="rhaB"
                     /locus_tag="RPM73_003753"
     CDS             3997..5466
                     /gene="rhaB"
                     /locus_tag="RPM73_003753"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDX6522063.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5463..6722
                     /gene="rhaA"
                     /locus_tag="RPM73_003754"
     CDS             5463..6722
                     /gene="rhaA"
                     /locus_tag="RPM73_003754"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDX6522064.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            6897..7721
                     /gene="rhaD"
                     /locus_tag="RPM73_003755"
     CDS             6897..7721
                     /gene="rhaD"
                     /locus_tag="RPM73_003755"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDX6522065.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            7731..>8009
                     /gene="rhaM"
                     /locus_tag="RPM73_003756"
     CDS             7731..>8009
                     /gene="rhaM"
                     /locus_tag="RPM73_003756"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDX6522066.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPV"
BASE COUNT         1894 a         2084 c         2154 g         1877 t
ORIGIN      
        1 caggcggcaa atgattattt tttcgccgca aaacgtgccg ctgcttcgtc ccagttcacc
       61 acgttccaga actctttaat gtagtccgga cggcggttct ggaatttcag gtagtaagca
      121 tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc
      181 atcagcggag aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc
      241 agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag
      301 ttatcaacgg agccgaagtc acgttcgata gccgctttca ggtcaccctg cagggtggtg
      361 ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc
      421 agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc
      481 aggttggcaa attctggcag gctttccagc gccgcgttgg cgttgtttac gtaggtctga
      541 tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg
      601 taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg
      661 tcgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa
      721 tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt
      781 gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc
      841 gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg aagcgacgaa
      901 aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat
      961 cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg
     1021 ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt gggccatcag
     1081 cgccctgtta ctaccggatt tctgggcgta ttacagctcg tttagtctct ctacgctgct
     1141 gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactatg gcctgaccat
     1201 gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg
     1261 tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ctgattaaca ccgaaggcgg
     1321 acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg taactcgagc
     1381 cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg
     1441 gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc
     1501 cgcaaaaccg atgcatgaag ccgctgccgc actaggcgtc gatccactgt atgtcgctct
     1561 gccaagctat gtcatcatca tgggcggcgg cgcgatcatc aacctcggtt tctgttttat
     1621 tcgtctggca aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct
     1681 gatcactcac aatgtgttac tctcagcact gggcgggttg atgtggtatc tgcaattctt
     1741 tttctatgcc tggggccacg cccgcattcc ggcgcaatat gactacatca gttggatgct
     1801 gcatatgagc ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa
     1861 caatgcagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc
     1921 cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca
     1981 ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc
     2041 aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg
     2101 cgcatgacac actctgacct gtcgcagata ttgattgatg gtcattccag tctgctggcg
     2161 aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc
     2221 aaagggactt ttcaggctag ccgccagccg ggtaatcagc ttatccagca acgtttcgct
     2281 ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg
     2341 cccgaacagc aactcagcca tttcgttagc aaacggcaca tgctgactac tttcatgctc
     2401 aagctgaccg ataacctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg
     2461 ttgccctgcg ctggcgctaa atcccggaat cgccccctgc cagtcaagat tcagcttcag
     2521 acgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg
     2581 tttatcgtca gcatgaatgt aaaagagatc gccacgggta atgcgataag ggcgatcgtt
     2641 gagtacatgc aggccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg
     2701 ttcagcaaag acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc
     2761 aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga
     2821 acgaagttga ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca
     2881 gaaaaccatc ccgtccctga cgaatatcac gcggtgacca gttaaactct cggcgaaaaa
     2941 gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata ggcgatgtca gtaacgctgg
     3001 cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc aggtatcgct
     3061 gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa
     3121 attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca
     3181 gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca
     3241 gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt
     3301 ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt
     3361 taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc
     3421 cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat
     3481 cggtatgttc atacagatgc cgatcatgat tgcgtacgaa acagaccgtg ccaccggtga
     3541 tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt
     3601 catgaaaatc atgatgatgt tcaggaaaat ccgcctgagg gagccggggt tctatcgcca
     3661 cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct
     3721 gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga
     3781 cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc
     3841 ggtgagcatc acatcaccac aatgaagcaa attgtgaaca tcatcacgtt catctttccc
     3901 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcacgaattc aggcgctttt
     3961 tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc
     4021 gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc
     4081 agcctgacgc tgcgcgaaat ccatcgtttt aaaaatgggc tgcatagcca gaacggttat
     4141 gtcacctggg atgtggatag cctggaaagt gccattcgcc ttggattaaa catggtgtgt
     4201 gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg
     4261 ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgcaccaat
     4321 ggcctaatgg cgcaggcaca gcaacaactc ggcaaacgcg atatttatca acgtagcggc
     4381 atccagtttc tgcccttcaa tacgctttat cagttgcgtg cgctgacgga gcaacaacct
     4441 gaacttattc cacacattgc tcacgctctg ctgatgccgg attacttcag ttatcgcctg
     4501 accggcaaga tgaactggga gtacaccaat gccaccacca cacaactggt caatatcaat
     4561 agcgacgact gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt
     4621 cgcccgacgc atccgggtaa tgtcattggg cactggattt gcccgcaggg taatgagatt
     4681 ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac
     4741 ggttcacgcg ccgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag
     4801 acgccattta ccaatgacac ggcgctggca gccaacatca ccaatgaagg cggggcggaa
     4861 ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag
     4921 gaacggcaaa tcaacgatct cccggcgctt atcgccgcga cacaggcact tccggcctgt
     4981 cgcttcatca tcaatcccaa tgacgatcgc tttattaatc ctgaagcgat gtgcagcgaa
     5041 attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc tgaactggcg
     5101 cgctgtattt tcgacagtct ggcgctgctg tatgccgatg tgttgcatga gctggcgcag
     5161 ctgcgcggtg aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacacgctg
     5221 ctcaaccagc tatgcgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc
     5281 tcaacgctcg gcaatatcgg catccagtta atgacgctgg atgaactcaa caatgtggat
     5341 gatttccgtc aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac
     5401 agtgaaattg cccactatgt ggcgcagatt cactctacac gacagacaaa ggagctttgc
     5461 gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcggtgg
     5521 ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct
     5581 ggcagggcga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggtgggattc
     5641 aagctactgg taattatccg ggcaaagcgc gtaatgccag tgagctacgt accgatctgg
     5701 aacaggctat gcggctgatt ccggggccga aacggcttaa tttacatgcc atctatctgg
     5761 aatcagatac gccagtctcg cgcgatcaga tcaaaccaga gcacttcaaa aactgggttg
     5821 aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgcttt tcgcatccgc
     5881 taagcgccga tggctttacg ctttcccatg ccgacgacag cattcgccag ttctggattg
     5941 atcactgcaa agccagccgt cgcgtttcgg cctattttgg cgagcaactc ggcacaccat
     6001 cggtgatgaa catctggatc ccggatggta tgaaagatat caccgttgac cgtctcgccc
     6061 cgcgtcagcg tctgctggca gcactggatg aggtgatcag cgagaagcta aaccctgcgc
     6121 accatatcga cgccgttgag agcaaattgt ttggcattgg cgcagagagc tacacggttg
     6181 gctccaatga gttttacatg gggtatgcca ccagccgcca gactgcgctg tgcctggacg
     6241 ccgggcactt ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg
     6301 tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtagtgc
     6361 tgctggatga tgaaacccag gcaattgcca gtgagattgt gcgtcacgat ctgtttgacc
     6421 gggtgcatat cggccttgac ttcttcgatg cctctatcaa ccgcattgcc gcgtgggtca
     6481 ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct accgccgagc
     6541 tgcgcaagtt ggaagcggcg ggcgattaca ctgcgcgtct ggcactgctg gaagagcaga
     6601 aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca acgtcacgat accccaacgg
     6661 gtagcgaatg gctggagagc gtgcgggctt atgagaaagc gattttgagc cagcgtgggt
     6721 aaacactgcc ggatgcggcg cgagcgcctt atccggccta caggtcggca acagttgtag
     6781 gcctgataag acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cacgagcgcc
     6841 ttatccggcc tacgggtcgt gcatctgaca acaccgaatt tacaggaaca cagaacatgc
     6901 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
     6961 tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcttggat gacgccgata
     7021 tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc agccagccca
     7081 tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
     7141 tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
     7201 accatattct ctgggggtta accaacgaag ccgttcccac ttccgaactt ccggctcact
     7261 tcctttccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
     7321 gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta
     7381 ctcgccaact gtgggaaggc agcaccgagt gtctggtagt gttcccggat ggcgttggca
     7441 ttttgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc
     7501 aaaaacattc gctggtgttg tggtccttcc acggcgtctt cggcagcggg ccgacgctgg
     7561 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
     7621 attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc
     7681 gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca
     7741 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc
     7801 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
     7861 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
     7921 ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta
     7981 tgcccgctaa cccggataac agcccggtg
//
LOCUS       DAPPNJ010000198         7929 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000198 DAPPNJ010000000
VERSION     DAPPNJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.200"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..337
                     /locus_tag="RPM73_003757"
     CDS             <1..337
                     /locus_tag="RPM73_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDX6522067.1"
                     /translation="ALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIPDRVVEQL
                     LAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIYQSVAAEL
                     YAHDLERLC"
     gene            437..1650
                     /locus_tag="RPM73_003758"
                     /pseudo
     CDS             437..1650
                     /locus_tag="RPM73_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1877..3142
                     /locus_tag="RPM73_003759"
     CDS             1877..3142
                     /locus_tag="RPM73_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6522068.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSTLATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            3154..4020
                     /gene="ydiB"
                     /locus_tag="RPM73_003760"
     CDS             3154..4020
                     /gene="ydiB"
                     /locus_tag="RPM73_003760"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDX6522069.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            4051..4809
                     /gene="aroD"
                     /locus_tag="RPM73_003761"
     CDS             4051..4809
                     /gene="aroD"
                     /locus_tag="RPM73_003761"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDX6522070.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            4953..6548
                     /locus_tag="RPM73_003762"
     CDS             4953..6548
                     /locus_tag="RPM73_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDX6522071.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            6562..7713
                     /locus_tag="RPM73_003763"
     CDS             6562..7713
                     /locus_tag="RPM73_003763"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDX6522072.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(7756..>7929)
                     /locus_tag="RPM73_003764"
     CDS             complement(7756..>7929)
                     /locus_tag="RPM73_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDX6522073.1"
                     /translation="SQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQE
                     ILSEFPQPVSLAG"
BASE COUNT         2050 a         1815 c         1912 g         2152 t
ORIGIN      
        1 ggcacttcgc catatttttg ccatgactat tgatgaatgc gcgacctgga ttgcccagac
       61 cggtgacagt gaaagctggc gtcagtggga aaatggcaaa tgcgccattc ctgatcgtgt
      121 agtcgagcaa ctgttggcta tgcgtcagca acgaaaaaaa catcttcatg ccatcataga
      181 gaaaataaat aaccgcatcg gcaacaacac catgcgtttt ttccccgact taaccgcgtt
      241 tcaacaagtc taccctgatg gcaattttat tgactggaag atctatcaat cggtagccgc
      301 tgaattatat gcacacgatc tggagcggct ttgttaattt ctccacaaaa aggaattgtc
      361 gttgttacaa caataatgaa cggatactga cacaacatcg ctacactttt taaagcacct
      421 ttgctaagta gaacctatga aaaatcccta ttaccctacc gcacttgggt tgtattttaa
      481 ttacctggtg catggtatgg gcgtcatttt gatgagcctg aatatggcct cgctggagac
      541 actttggcag actaatgccg cgggtgtctc gatagttatc tcatcgctgg gcattggtcg
      601 attaagtgtc ttgctttttg caggattatt atccgatcgc tttggtcgcc gcccttttat
      661 catgctcggg atgtgctgct atatggcctt cttttttggc atcctgcaca ccaataacat
      721 cattatcgct tatgtttttg gctttctggc aggaatggca aacagttttc tcgatgcagg
      781 cacttatccc agtttgatgg aagcttttcc acgctcacct gggacagcca atattttaat
      841 taaagcattt gtttccagcg gacaattttt attaccgcta atcattagcc tgttagtgtg
      901 ggctgaactg tggttcggtt ggtcctttat gattgctgca ggcattatgt ttattaacgc
      961 tctgttttta taccgttgta cgttcccacc ccatccgggt catcgcttac ctgtcataaa
     1021 gaaaaccacc agctctacgg aacatcgctg ttcaattatc gatttagcca gttatacctt
     1081 atatggctat atctcaatgg caacgtttta tctggttagc cagtggctgg cacagtacgg
     1141 acaatttgtt gcaggcatgt catacactat gtcgattaaa ctactcagta tctacaccgt
     1201 gggttcgctg ctttgtgtat ttattaccgc tccactcatt cgtaataccg ttcgcccaac
     1261 aacattactg atgctgtaca cctttatctc atttattgct ctgtttaccg tctgcctgca
     1321 tcccacattt tatgtggtga taatatttgc ttttgtcatt ggtttacctc tgctggaggt
     1381 gttgtgcaaa ttggcctgac gttaatggct gaacgtttcc cttacgctaa aggtaaagct
     1441 acagggatct attacagtgc gggcagtatt gcgaccttta ctattccgtt gattacggct
     1501 catctgtccc aaagaagtat tgccgatatt atgtggttcg ataccaccat cgctgccatc
     1561 ggttttttac tggcactgtt tatcggctta cgcagccgca aaaaaacgcg gcatcagtcg
     1621 ctaaaggaaa atgtcgctcc gggtgggtaa tgcaatattc ttttcaggtc atgcaagatc
     1681 ttacggataa ataactcttt ctgcgctaac taaggaaaat cgcgatcaaa aacaaactat
     1741 gacatgcaat attcctggaa acataaactt tatgccatgt acccagggaa aatcatcttc
     1801 agtatagtaa ttatgtaaac cgtcggagaa caatacgtac ggtaacgaaa ttatctttca
     1861 gcaaggagct gtgaaaatgt ctcaaaataa ggctttcagc acgccattta tcctggctgt
     1921 tctttgtatt tacttcagct acttcctgca cggcattagt gttattacgc ttgcccaaaa
     1981 catgtcatct ctggcggaaa agttttccac tgacaacgcg ggcattgcct atttaatttc
     2041 cggtatcggt ttggggcgat tgatcagtat tttattcttc ggtgtgatct ccgataagtt
     2101 tggtcgtcgg gcggtgatat taatggcagt aataatgtat ctgctattct tctttggtat
     2161 tcccgcttgc ccgaatttaa ctctcgccta cggtctggca gtgtgcgtag gtatcgctaa
     2221 ctcagcgctg gatacgggtg gctaccccgc gctcatggaa tgctttccga aagcctctgg
     2281 ttcggcggtc atactggtta aagcgatggt gtcattcgga caaatgttct acccaatgct
     2341 ggtgagctat atgttgctca ataacatctg gtacggctat gggctgatta ttccaggcat
     2401 cctatttgta ctgatcacgc tgatgctgtt gaaaagcaaa ttccccagcc agttggtgga
     2461 cgccagcgta gctaatgaat taccgcaaat gaacagcaaa ccgttagtct ggctggaagg
     2521 tgtttcatcg gtactgttcg gtgtagccgc attctcgacc ttttatgtga ttgtggtgtg
     2581 gatgcccaaa tatgcgatgg cttttgctgg tatgtcagaa gctgaggcat taaaaaccat
     2641 ctcttattac agtatgggct cgttggtctg tgtctttatt tttgccgcac tactgaaaaa
     2701 aatggtccgg cccatctggg ctaatgtatt taactctaca ctggcaacaa taacagcagc
     2761 cattatctac ctgtaccctt ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt
     2821 ctcagcagct ggcggcattt tacagctcgg tgtttcggtc atgtcagagt tttttccaaa
     2881 aagcaaagcc aaagtcacca gtatttatat gatgatgggt ggactggcta actttgttat
     2941 tccgctgatt accggttatc tgtcgaacat cggcctgcaa tatatcattg ttctcgattt
     3001 tactttcgcg ctgctggccc tgattaccgc aattattgtt tttatccgct attaccgcgt
     3061 ttttattatt cctgagaatg atgtgcggtt tggcgagcgc aaatttagca cccgattaaa
     3121 cacaattaag catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg
     3181 attgggctga tggcctatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc
     3241 ttagaaaaag cgggattgcc atttacctat atggcctttg aagtggataa cgatagtttt
     3301 cccggagcaa ttgaaggatt aaaagccctc aaaatgcgcg gaactggtat atcaatgccg
     3361 aacaaacaac tggcgtgtga atatgttgat gaattaacgc cagcagccaa actggtgggt
     3421 gccatcaaca ccatcgttaa tgatgatggt tacctgcgcg gctataacac cgacggcacg
     3481 ggccatattc gagccattaa agagagcggt tttgatatca aaggcaaaac aatggtgctg
     3541 ttaggggccg gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa
     3601 gaaattaaac tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag
     3661 cgggttaacg aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc
     3721 tttgctgaag ccctggcttc cgccgacatt ttaaccaatg gcacaaaagt gggtatgaaa
     3781 ccccttgaga atgaatcatt agttaatgat atcagtctgt tacatccggg actgctggtc
     3841 actgaatgcg tgtataaccc gcatatgacg aagttattgc agcaggcgca acaagctggt
     3901 tgcaaaacga ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta
     3961 tggactggca aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga
     4021 caggctgacc gcgtgcagaa agggtaaaaa atgaaaaccg taactgtaaa agatctcgtc
     4081 attggtacgg gcgcacctaa aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg
     4141 aaatccgaag ctctcgccta tcgtgaagcg gactttgata ttctggaatg gcgtgtggac
     4201 cactttgccg acctctccaa tgtggagtct gtcatggcgg cggcaaaaat tctccgtgaa
     4261 accatgccag aaaaaccgct gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag
     4321 gcgatttcca ccgaggctta tattgctctc aatcgtgcag ccatcgacag cggcctggtt
     4381 gatatgatcg atctggagtt atttaccggc gatgatcagg ttaaagaaac cgtcgcctac
     4441 gcccacgcgc atgatgtgaa agttgtcatg tccaaccatg acttccataa aacgccggaa
     4501 gccgaagaaa tcattgcccg tctgcgcaaa atgcagtcct tcgacgccga tattcctaag
     4561 attgcgctga tgccgcaaag taccagcgat gtgctgacgt tgcttaccgc gaccctggag
     4621 atgcaggagc agtatgccga tcgtccaatc atcacgatgt cgatggcaaa aactggcgta
     4681 atttctcgtc tggctggtga agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa
     4741 gcctctgcac cagggcaaat ctcggtaaat gatttacgca cggtattaac tattttacac
     4801 caggcataag caataatatt tcggcgggaa caccctcccc gccgaactta aaaaatatat
     4861 ttaaccgtat ttaataaaaa tatttcgtga gtctctgtgc gctaattctc catctggcgt
     4921 agggaaaatc acatctgaat caggaattaa caatgaaacc tgtaaaacca cctcgtatta
     4981 atggacgagt gccggtcctg tcggcacagg aagcggtgaa ttatattccc gacgaagcaa
     5041 cactttgtgt gttaggcgct ggcggcggta ttctggaagc caccacgtta attactgctc
     5101 ttgctgataa atataaacag actcaaacgc cacgtaattt atcgattatt agtccaacag
     5161 ggcttggcga tcgcgccgac cgcggtatta gtcctctggc gcaagaaggt ctggtgaaat
     5221 gggcattatg tggtcactgg ggacaatcgc cgcgtatttc tgaactcgca gaacaaaata
     5281 aaattattgc ttataactat ccacaaggtg tacttacaca aaccttacgc gccgccgcag
     5341 cccaccagcc tggtattatt agtgatattg gcatcgggac atttgtcgat ccacgccagc
     5401 aaggcggcaa actgaatgaa gtcacgaaag aagacctgat taaactggtc gagtttgata
     5461 acaaagaata tctctattac aaagcgattg cgccagatat cgccttcatt cgcgctacca
     5521 cctgcgacag tgaaggctac gccacttttg aagatgaggt gatgtatctc gacgcattgg
     5581 ttattgccca ggcggtgcac aataacggcg gtattgtgat gatgcaggtg cagaaaatgg
     5641 ttaagaaagc cacgctgcat cctaaatctg tccgtattcc gggttatctg gtggatattg
     5701 tggtggtcga tccggatcaa agccaactgt atggcggtgc gccggttaac cgttttattt
     5761 ctggtgactt cacccttgat gacagcacca aacttagcct gcccctaaac caacgtaaat
     5821 tagttgcgcg gcgtgcatta ttcgaaatgc gtaaaggcgc ggtggggaat gtcggcgtcg
     5881 gtattgctga cggtattggc ctggtcgccc gggaagaagg ttgtgctgat gactttattc
     5941 tgacggtaga aacaggtccg attggcggaa ttacttcaca gggtatcgcc tttggcgcga
     6001 acgtgaatac ccgcgccatt ctggatatga cgtcccagtt tgatttttat cacggtggtg
     6061 gtctggatgt ttgttatttg agttttgctg aagtcgacca gcacggtaac gtcggcgtgc
     6121 ataaattcaa tggcaaaatc atgggtaccg gtggatttat tgatatcagt gccacctcga
     6181 agaaaattat tttctgcggc acgttaactg cgggcagttt aaaaacagaa attgccgatg
     6241 gcaaattgca tatcgtccag gaaggacggg taaataaatt tattcgggaa ctaccggaaa
     6301 ttacttttag cggaaaaatc gcgctcgaac ggggactgga tgttcgttac atcaccgaac
     6361 gtgctgtatt cacgctgaaa gaagacggcc tgcatttaat cgaaatcgcc cctggcgtcg
     6421 atttacaaaa agatattctc gacaaaatgg acttcacccc agtgatttcg ccagaactca
     6481 aactgatgga cgaaagatta tttatcgatg cggcgatggg ttttgtcctg cctgaagcgg
     6541 ctcattaaca ggagtataat gatggatttt tctttaactg aagaacaaga actgctgctg
     6601 gccagtattc gcgaactgat tacgactaac tttccggaag agtatttccg cacctgcgat
     6661 caaaacggga catatccgcg tgagtttatg cgggcgctgg cggataacgg tatttccatg
     6721 cttggcgtgc cggaagaatt tggtggtatc cctgcggatt acgtcaccca aatgctggcg
     6781 ctgatggaag tgtcaaaatg cggtgctccg gcatttttaa ttaccaacgg tcaatgtatt
     6841 cacagtatgc gccgtttcgg ttctgcagag cagctacgta aaacggcaga gagcaccctg
     6901 gaaacgggtg atcccgccta tgccctggcg ttgacggaac caggtgctgg ctcagataac
     6961 aacagtgcca ctaccactta cacgcgtaaa aacggcaagg tctacatcaa cggacaaaaa
     7021 acctttatta ccggtgcgaa agagtacccg tatatgctgg tgctggcgcg cgatccgcaa
     7081 ccgaaagatc ccaaaaaagc cttcaccctg tggtgggtcg actccagtaa gcccggcatt
     7141 aagattaacc cgctgcataa aatcggctgg catatgctca gcacctgcga agtctatctc
     7201 gacaatgtgg aagttgaaga gagcgacatg gtgggcgaag aaggaatggg tttcctcaat
     7261 gtgatgtaca actttgagat ggagcgcctg atcaacgccg cgcgcagcac cggctttgcc
     7321 gaatgcgcct ttgaagatgc cgcccgctat gccaaccaac gtatcgcttt tggtaagccc
     7381 attggtcata accagatgat ccaggaaaaa ctggcgctga tggcgattaa gatcgacaac
     7441 atgcgcaaca tggtgctgaa agtggcatgg caagccgatc agcatcagtc actgcgtacc
     7501 agcgcggcgc tggcaaaact gtattgcgca cgtaccgcaa tggaagtcat tgatgatgcg
     7561 attcaaatca tgggcggtct gggctatacc gatgaggcgc gcgtctcccg cttctggcgt
     7621 gatgtccgtt gtgaacgtat cggcggcggt acagacgaaa ttatgattta cgttgcgggt
     7681 cggcagatcc tgaaagatta tcagaacaaa taatctgcag gcggcgcagc ttcttaacaa
     7741 actgcgccgc cagatttacc cggcaagact taccggttga ggaaattcag ataggatttc
     7801 ttgttccgca aaacagtttt taaattgcct gcggtaatcg ctgggcgaac aaccgacatg
     7861 gcgcaaaaac agtttggcaa agtgatcgac attttcataa cccacacgcc aggagatctc
     7921 tgcctgtga
//
LOCUS       DAPPNJ010000199         7794 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000199 DAPPNJ010000000
VERSION     DAPPNJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.201"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            83..1474
                     /gene="xanP"
                     /locus_tag="RPM73_003765"
     CDS             83..1474
                     /gene="xanP"
                     /locus_tag="RPM73_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDX6522074.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            1595..3304
                     /locus_tag="RPM73_003766"
     CDS             1595..3304
                     /locus_tag="RPM73_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDX6522075.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(3357..5675)
                     /gene="yicI"
                     /locus_tag="RPM73_003767"
     CDS             complement(3357..5675)
                     /gene="yicI"
                     /locus_tag="RPM73_003767"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDX6522076.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKIGNLSARVSKGEFWSLDFLSNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5685..7067)
                     /locus_tag="RPM73_003768"
     CDS             complement(5685..7067)
                     /locus_tag="RPM73_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDX6522077.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7360..7454
                     /locus_tag="RPM73_003769"
     tRNA            7360..7454
                     /locus_tag="RPM73_003769"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7394..7396,aa:Sec,seq:tca)
BASE COUNT         1860 a         2074 c         1972 g         1888 t
ORIGIN      
        1 tttagatgag gatgtggcaa acgtttgctt ttctctttcg gtcagctaaa atgcccgctt
       61 tgctaccacg ggattgtttt cgatgtctgt ttccaccctc gagtcagaaa atgcgcaacc
      121 ggttgcgcag actcaaaaca gcgaactgat ttaccgtctt gaagatcgtc cgccgcttcc
      181 tcaaaccctg tttgccgcct gtcagcatct gctggcgatg ttcgttgcgg tgatcacgcc
      241 agcgctatta atctgccagg cgctgggttt accggcacaa gacacgcaac acattattag
      301 tatgtcgctg tttgcctccg gtgtggcatc gattattcaa attaaggcct ggggtccggt
      361 tggctccggg ctgttgtcta ttcagggcac cagcttcaac tttgttgccc cgctgattat
      421 gggcggtacc gcgctgaaaa ccggtggtgc tgatgttcct accatgatgg cggctttgtt
      481 cggcacgttg atgctggcaa gttgcaccga gatggtgatc tcccgcgttc tgcatctggc
      541 gcgccgcatt attacgccgc tggtttctgg cgttgtggtg atgattatcg gcctgtcgct
      601 aattcaggtt gggttaacgt ccattggcgg cggttacgca gccatgagcg ataacacctt
      661 cggcgcaccg aaaaatctgc tgctggcagg cgtggtctta gccttaatta tcctgcttaa
      721 ccgtcaacgt aacccttact tacgcgtggc ctcactggtg attgcgatgg cggccggata
      781 tgcgctggcg tggtttatgg gcatgttgcc agaaagcaac gaaccgatga cgcaagaact
      841 gattatggtg ccaacgccgc tctattacgg tcttggcatt gaatggagtc tgctgctgcc
      901 gctgatgctg gtctttatga tcacttcgct ggaaaccatt ggcgatatca cggcgacctc
      961 tgacgtttcc gaacagccag tgtccggtcc gctgtacatg aaacgcctga aaggcggcgt
     1021 gctggcaaac ggcctgaact cgtttgtttc ggcggtgttt aacaccttcc cgaactcctg
     1081 cttcgggcaa aacaacggag tgatccagtt gactggtgtt gccagccgct atgtcggttt
     1141 tgtcgtcgcg ctgatgttga tcgtgctggg tctgttcccg gcagtgagcg gttttgtaca
     1201 acacattcca gaaccggttc tgggcggcgc aacgcttgta atgtttggca ccatcgccgc
     1261 ctccggtgtg cgtatcgttt ctcgtgagcc gctgaaccgt cgggcgattc tgattatcgc
     1321 gctgtcgctg gcggttggtc tgggcgtgtc tcagcagccg ctgattttgc agtttgcccc
     1381 tgaatggctg aaaaacctgc tctcctccgg gatcgccgcg ggcggtatta ctgccatcgt
     1441 gctgaatctg attttcccac cagaaaaaca gtaattcact ggcggcggta agataattac
     1501 cgccgcagtc tcacctcttt caccattccc gccttgagga ttgcgcgtaa atcgtgcata
     1561 actcccttat gtgcatttca cgggatggaa gaccatgaaa tttattggga agctgcttct
     1621 ctacattctc atcgctctgt tagtggtgat cgctggcctc tattttcttc tgcaaacccg
     1681 ctggggagca gaacatatca gcgcatgggt ttccgagaat agcgactatc atctggcctt
     1741 cggggcgatg gatcaccgtt tttccgcgcc atctcatatc gtgctggaga acgtcacgtt
     1801 tggtcgtgat ggtcagcccg cgaccctggt ggcaaaaagt gtcgacattg cgctaagcag
     1861 tcggcaactg accgaaccac gccatgtcga taccatcctg ctggaaaacg ggacgctgaa
     1921 tctcaccgac cagaccgcgc cgctaccgtt caaagccgat cgtctgcaac tgcgtgatat
     1981 ggcgtttaat agcccgaata gcgaatggaa actgagcgcg cagcgggtaa atggcggcgt
     2041 ggttccgtgg tcaccagaag ccggtaaagt gctgggtacg aaggcgcaga ttcagtttag
     2101 tgccggatcg ctttcgctca atgatgttcc tgccaccaat gtactgattg aaggcagtat
     2161 tgataacgat cgcgttacgc tgactaacct gggtgccgac atcgcccgcg ggacattaac
     2221 cggaaacgcg cagcgtaacg ccgacggcag ctggcaagtg gaaaatctgc gcatggcgga
     2281 tatccgtcta caaagcgaaa aatcgctaac cgacttcttt gcgccattac gctctgtccc
     2341 gtcgttgcag attggtcgcc tggaagtgat cgatgctcgt ttgcaaggtc cggactgggc
     2401 ggtgaccgac ctcgatctca gcttgcgcaa catgaccttc agtaaagatg actggcagac
     2461 acaagaaggc aaactgtcga tgaacgctag cgagttcatt tatggttcgc tgcatttatt
     2521 tgacccgatt ataaacgcgg aattttcccc gcagggcgta gcgctgcgcc agttcaccag
     2581 ccgctgggaa gggggtatgg tcagaacgtc agggaactgg ctgcgtgacg ggaaaacgtt
     2641 gatccttgat gatgcggcaa ttgccgggct ggaatatacc ttgccgaaaa actggcaaca
     2701 gttgtggatg gaaacgacac ccggttggtt aaacagcctg caactgaaga gatttagcgc
     2761 cagccgcaat ctgatcattg atatcgaccc tgacttcccg tggcagctca ccgcgctcga
     2821 tggttacggt gccaacctga cgctggttac cgatcataaa tggggcgtct ggagtggctc
     2881 ggcgaatctg aatgccgccg ccgcgacatt caatcgtgtt gatgttcgtc gcccgtcgct
     2941 ggcgctgacc gccaacagca gcacggtgaa tatcagcgaa ctgagtgcat ttactgaaaa
     3001 aggcattctg gaagccaccg ccagtgtttc acaaacgcca caacgtcaga cacatatcag
     3061 cctgaatgga cgcggtgtgc cggtgaatat tttgcaacag tggggatggc ctaaattacc
     3121 gttgactggc gacggcaata ttcagcttac cgccagtggc gatattcagg ccaatgtccc
     3181 gttgaaacct acggttagcg ggcaattgca tgccgtgaac gccgcaaagc agcaagtgac
     3241 tcaaaccatg aatgctggca tcgtttccag cggtgaagtt acatcgacgg agccggtgcg
     3301 gtaaacatca atctcgccgg gtacgactca gacgtacccg gcattccatc aatagattac
     3361 aacgtaattg tcagcgcatt cccttgaggc ttcaccacca gcccctgctc actttcagcc
     3421 tgcgaaccgt cttgcagacc atttactttc acaacattgc gcaggcacag cgtccagttc
     3481 ttcgcctcgc ccgcaccagt cacagtaatc gtgttgccag tacgtgctgc ttttaaagta
     3541 aagatcaccg atccgtcagt agcgggcact tcacagacgg cttcatgccc gtcttgcaga
     3601 ttaaagaggt ggaatgccgt gccttcgtgc cacacgtaat cgggacgttg atcgttgttg
     3661 cccagcgcca gtagggtgtt atcacgcaca taaatgggca gactcaggaa gctgtgctgc
     3721 tgtttatgcc agcgactacc gtcgagctca tcgttgtgcc acaggtgtgt ccagcgacct
     3781 tccggcaggt agaactgcac atcgcccgct tcagtgaaca ccggcgcaac catcacgttg
     3841 tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc
     3901 atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac gcgcagcttc acgatacaga
     3961 tacggcatca tgcggcattt cagttgcgtg aagaagcgca ccacatcaca ggactcatca
     4021 tcgtaggccc acggcacacg ataagatttg ctaccgtgta aacggctatg gctggagagc
     4081 aaaccaaacg cgcaccagcg tttgtaaacg tgcgccggag cggtattttc aaagccgccg
     4141 atatcgtggc tccagaagcc aaaacctgaa aggccaatag acaaaccacc gcgcaggctt
     4201 tccgccattg attcgtagtt agcgtaacaa tcgccacccc agtgtaccgg gaatttctgc
     4261 gcaccgacgg aggccgagcg ggcaaacaag acagcttctt cctcaccaac ggtgtccttg
     4321 agcacgttcc acaccagttc gttgtagatg tacgcataat ggttatgcat tttctgcgga
     4381 tcggaaccgt caaaccactg aacatcagtt gggatacgtt cgccaaagtc ggtcttaaag
     4441 caatcaacgc ccatcgcgac cagacctttc agtttgtcgg cgtaccattt gcaggcatcc
     4501 ggattggtaa agtcataaat cgccagacct ggctgccatt tatcccactg ccatagcgaa
     4561 ccgtccgggc gtttgagtaa atagcctttc tcttgtaact ctttaaagac gggggatttt
     4621 tgaccgatat aggggttaat ccagacgcag attttcagtc ctttcgcttt caggcggcgg
     4681 atcatccctt ccgggtcagg gaaagtcagc gggtcccact caaaatcgca ccactggaag
     4741 gctttcatcc agaaacagtc aaagtggaaa acatgcagcg gcagattgcg ttccgccata
     4801 ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg tggtaaatga agtggttagc
     4861 cacaggccga aggaccacgc gggcggcagc gccggacgac cagtaaagcg ggtataacga
     4921 tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt attcgagata ttcactctca
     4981 acgctgaact gcactttgga gactttctcc gatcccactt caaaagagac acactgggga
     5041 tgattgacca gtaccccata accacggtta gtcatgtaga acgggatatt tttatacgcc
     5101 tgttcagtac ttgtgccgcc gtcccggttc caggtctcta ccgtctggcc attgcgcacc
     5161 agggcagtaa agcgctctcc cagaccgtaa actgtttcgc caacgccaag atcaagccgc
     5221 tcaaacatat aattgcgttg attattcgtg tcctgcacgt agccattatt tttcacctga
     5281 ctaccggtaa tacgttcgcc gttgctcaga aaatccagtg accagaactc acctttgctg
     5341 acacgcgcgc ttaagttgcc aattttaaac tcagcataac gttctgtgtt ttcgattgtg
     5401 accttcacgt cctgcaaaat attgagcgga taatgaggac cgttattcag cgccccctga
     5461 aaatgctcaa tccgcacacc gacaatacct tcctgtgggg agaaaaagcg caacgtaaat
     5521 aaaggcgtat caagctgcca ggtacgttca cgcacatcac ggggggcagc atagaccacc
     5581 atttcattat cctgctgttc aacctcgaac acctgaagcg ggtgaatcaa attgaggcca
     5641 ggttgaatca accagtttcc atcgctaatt ttcattgccg ttccttagtt ctgcacttct
     5701 tgagaggtga attgttgctg gcaacggcgt ttaccctgag ccagctgttc cataacggtt
     5761 ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga taatcgcgct cagcaaataa
     5821 cagatcgccg gaacaatcgt gaatagcgca ataatgatgc taatcgtggc gctgttctgc
     5881 gctttttctg ccgcatcata tccgccataa gccagcatcc agccgataag agcgccgccg
     5941 aaggccaacc ccagtttgag cacaaacagc gtgccagcaa aactgatccc ggtcaggcgt
     6001 ttaccattgc accactcgcc gtagtcgacg gtatcggaca tcattaccca ctggataggt
     6061 gtcaccagtt gatgcaacac accaatcacg aagatgaaga caaacatagt gatgctggcc
     6121 tgcatgggaa caaagaacat cgcgaggcta atcactgcca gcagggcgtt cgtccaccag
     6181 aagatagtga ctttacattt ccagtcggtc agaggttttg ccagtgcgga accaatcagg
     6241 ttacccacgc aataagtggt gagaaaagcg acaaacactt ccggcgtgcc caaaatccat
     6301 gtgacgtaat acatcatcgc cccaccgcgt acgcacaccg ccaggatatt gaaaatggtt
     6361 agtaaaccga caatccgcca ctggtcgttt tgccagatat cacgtaaatc ttcccgcata
     6421 gacgttgttg taggtggtgc ttcaacgcgt tctttagtgg tgaagaaaca aaatgccagc
     6481 atcatgaatg ccaccacgga aaggaccgcg ataccgccct ggaaaccgag tggtttatta
     6541 tcaccgccaa ttaaattaac cagtggcatc atcagaacag tagaaagcat gcctcccgcg
     6601 gtcgccagca caaaacgcca ggattgcagc gagatacgct gagtcgggtc attggtgatt
     6661 acaccaccca atgcgcagta agggatattg acgacggtat aaagtaaggt aagtagggtg
     6721 taagtaattg ctgcatagat cattttgccg ttcatactga gatctggcgt gctataggcc
     6781 agtacacaga cgatcccgaa tggcagtgcg ccaaacagta cccacggacg aaatttaccc
     6841 cagcgagagc gcgttcgatc ggccaacaac cccatgcaag gatcggaaat cgcatccagt
     6901 gcacgagcga ccaaaaacat ggttccgaca aatccggcag gaatgccaaa aatatcggta
     6961 taaaagaaca tcatatataa cattacgtta tcgaaaataa tgtggctggc ggcgtctccc
     7021 atgccataac caattttctc tttaacggac aacacttcac tcttcatctg cttttccttg
     7081 atgctatagg ggtgctgaga ccgataacgc attttgtaaa ccatccctgt gagagcgaat
     7141 attccgatat ctggttatta atttatgatt cttgttttat gtgatcgtgg tagcgttaat
     7201 tccgctcata tatcattgta aaatatgggt tttatatgaa ctataatgct ttcgtgataa
     7261 tacgctgcgt gtattaggcg gaaaaaactg atctggggga tgtagaaact caaggaagta
     7321 gctataatgc gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg
     7381 aggcggctgg acttcaaatc cagttggggc cgccagcggt cccgggcagg ttcgactcct
     7441 gtgatcttcc gccaaaatgc ctctcctgac gttctccaac cccttgtaat actgtcaatc
     7501 cagatatttt ctactgctca atattcaatc ggcttcagga atacctggtc taaggttttg
     7561 tgatggacga tgagcggctg aagaatccgc ctgtcggttc ttctgtcctc gaacgcatcc
     7621 gcgatattca cgccagtatg cgccgtgttt atttgcaggt gatttgcccc cctgctgact
     7681 ctcagccaac tgtatcgcca ttaaggtgaa gatgttgttt tactcgcccc agaacaccct
     7741 tttttatacg actattctat ttctttaacc gctggcgaaa aaaactgact ataa
//
LOCUS       DAPPNJ010000200         7732 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000200 DAPPNJ010000000
VERSION     DAPPNJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.202"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            178..396
                     /locus_tag="RPM73_003770"
     CDS             178..396
                     /locus_tag="RPM73_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDX6522078.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            615..857
                     /locus_tag="RPM73_003771"
     CDS             615..857
                     /locus_tag="RPM73_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522079.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="RPM73_003772"
     CDS             complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="RPM73_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDX6522080.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="RPM73_003773"
     CDS             complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="RPM73_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDX6522081.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2745..3647)
                     /gene="fdoH"
                     /locus_tag="RPM73_003774"
     CDS             complement(2745..3647)
                     /gene="fdoH"
                     /locus_tag="RPM73_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDX6522082.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="RPM73_003775"
     CDS             complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="RPM73_003775"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6123..6125),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDX6522083.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLIANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6904..>7732
                     /gene="fdhD"
                     /locus_tag="RPM73_003776"
     CDS             6904..>7732
                     /gene="fdhD"
                     /locus_tag="RPM73_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDX6522084.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     "
BASE COUNT         1786 a         2000 c         2065 g         1881 t
ORIGIN      
        1 cctgggcgat aactaaacca tttttgagta ataaatgaaa caactgcaac actcttatta
       61 tttctggaaa acgcctcgca aaaacaaaac aacatgcaaa aatccgcttt ttttaaatga
      121 ttttccggcg taattttcgc acatacatta atgagtaata taggtaaggt ggtggtaatg
      181 gcgatgaata cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaaactt
      241 cggggctggc gtaaggtttc acgatctgca attttacgcg aagcggttga gcaatatctg
      301 gagcggcagc aatttccggt gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt
      361 ggtgttgatg atcagtgtaa ggagcataag gaatgaaaaa aatttttcat tcttgaatat
      421 aaaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg aacttataat
      481 aactgcaact gttacatcgt atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa
      541 atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc aaaaatgata
      601 ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt ttactcgatt
      661 tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg
      721 cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa
      781 ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc
      841 tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt
      901 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat
      961 atattgaagt ttattggtag ggctgataag cgcagcgcat caggcaatca tacgcttgtc
     1021 atcagtgaca tacccataac cttatcgatg tgttggaggg aagaacgagg caaacagaca
     1081 attgcggcat gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg tcacacccta
     1141 tgagctgtaa tccgggcgac cagcgccacc cggaaaacgt cagagattac tccccttcac
     1201 ccggaaacag gaacgggttg atggaactgc gggcatagcc ttcttgctcc attcgcgcgt
     1261 ccagtaccag agaagcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat
     1321 aaagaatctt caggtaagtg ccacagtcat cgcagctttc ggctttaatc gcggcctgtt
     1381 cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc
     1441 gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg
     1501 tgccaatttg caccatgctg gacaccggca tagaaccaca aaccgggcaa tattgacgtt
     1561 gctcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga
     1621 gcgacagtgc agcccagata aacggcgctt tatcgctgct gacggatgag aaatcagagg
     1681 caaacagtgc gctggccata tcttccagct cctgagttga tgccttctcc agattctcaa
     1741 tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaatcagc gccatcagca
     1801 gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg
     1861 cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg tacagcacca
     1921 cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt
     1981 ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca
     2041 ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga
     2101 ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg
     2161 cgggtgatgt ttcttcgccc aggcgctggt aacccatcct tccaccatgg cggtaatcgt
     2221 gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc
     2281 ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa
     2341 ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc
     2401 ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac
     2461 gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttgtgatg
     2521 ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg
     2581 cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa
     2641 caaaaagccc agcccgctca ctgccgccag gatgaagcag aaggcggtga tccagtggtt
     2701 gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg
     2761 tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc gacacccacg
     2821 tagtggaaga tactggctgc gaaggtagcc gcaaagccaa cagctgcgag cggtttccag
     2881 atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac
     2941 agatttggct tgtcagcatg gtgcagcacg tacataacgt gtgtaccacc gacgcctgcc
     3001 ggatcgtaca ggcccgcatt gtcgtaaccg cgggttttca gctccgccac gcgctcgctc
     3061 gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc
     3121 acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg
     3181 cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg
     3241 cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca
     3301 ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca gccgtctttg
     3361 cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat taccgtccac
     3421 gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg
     3481 cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat acaggtggtg
     3541 acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga
     3601 ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt
     3661 tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca
     3721 ccaacgaatg gcgttaacgt attggcaata aagccttttt tagctacgcc ttcatagccc
     3781 cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag tgtacgaata
     3841 cgtttggtca ccaccgcttt ggccttgata tagccacggt tggaggagac tttaacggta
     3901 tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct
     3961 ggttgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaaatg ctcggtcaga
     4021 cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct
     4081 ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc
     4141 ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca
     4201 aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc
     4261 gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac
     4321 tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg
     4381 cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc agacggatcg
     4441 gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag
     4501 cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag ttgttggcct
     4561 ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg
     4621 ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg
     4681 ttcagcacct gatccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg
     4741 aagataccgg agaggatctc accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc
     4801 cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag
     4861 gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca
     4921 ccgtggttct gccagaagtt agaggtttca gtgttcagcg ggtcgatggt gacgaggaac
     4981 ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc aacagggtta
     5041 aagccctggc agatatagcc attgaccttg ccttctttca tcatctcgaa gtattgcagg
     5101 acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt ttccgccgtc
     5161 gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag
     5221 tagtttacct ggccttccag cagcggtttt ggcgtgttgg cgataaggta ggtttgcaga
     5281 tcggtctgct tctcgcttgg cagcgtcatg taacctggca gactctgcga cagtagcccc
     5341 aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccggcc
     5401 atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca
     5461 acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg
     5521 gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt
     5581 tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc
     5641 ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag
     5701 gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag
     5761 ccgtaatcct cacgcactat caggctggcg ttggtatagg cttcggtgta ttcgcggttg
     5821 aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg
     5881 gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc
     5941 acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa cccgaccggg
     6001 tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc
     6061 cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg
     6121 tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc
     6181 gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag
     6241 cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc
     6301 atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa
     6361 cctggcgcac ggtattccgg aaacttcaga cggctttcag agtggatgaa atccaccagg
     6421 ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata
     6481 tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca
     6541 cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag cagtttatac
     6601 tgccgggttt ccgcgagcgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct
     6661 gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc
     6721 ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag
     6781 ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatcc
     6841 aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag
     6901 aaagtgaaaa aaacacagca aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt
     6961 caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa
     7021 gaagttcccg ttgcgctggt ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc
     7081 aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg
     7141 cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag
     7201 ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg
     7261 ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta
     7321 ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat
     7381 tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat gttgccatct
     7441 ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta
     7501 ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt
     7561 gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg
     7621 tctgccgcga ccacgcttgc tgtagaagtg gctgagcgtt gtaatctgac gctggtgggt
     7681 ttttgtaagc cgggtcgggc aacggtttat acccatccgc agcgtttgag ca
//
LOCUS       DAPPNJ010000201         7564 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000201 DAPPNJ010000000
VERSION     DAPPNJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.203"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            195..2618
                     /gene="pgaA"
                     /locus_tag="RPM73_003777"
     CDS             195..2618
                     /gene="pgaA"
                     /locus_tag="RPM73_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDX6522085.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2627..4645
                     /gene="pgaB"
                     /locus_tag="RPM73_003778"
     CDS             2627..4645
                     /gene="pgaB"
                     /locus_tag="RPM73_003778"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDX6522086.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVSIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4638..5963
                     /gene="pgaC"
                     /locus_tag="RPM73_003779"
     CDS             4638..5963
                     /gene="pgaC"
                     /locus_tag="RPM73_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDX6522087.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5965..6378
                     /gene="pgaD"
                     /locus_tag="RPM73_003780"
     CDS             5965..6378
                     /gene="pgaD"
                     /locus_tag="RPM73_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDX6522088.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6428..7216)
                     /gene="phoH"
                     /locus_tag="RPM73_003781"
     CDS             complement(6428..7216)
                     /gene="phoH"
                     /locus_tag="RPM73_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDX6522089.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT         2042 a         1753 c         1786 g         1983 t
ORIGIN      
        1 attggaatgg attttcgggc gagaaaagga ttttatatgg acactctgct catcatttct
       61 tcttctcatc atcaacaatt cacgtctctc ttccgcgttt aataacggat tatgaggtgc
      121 aaaaatatct ttcttttcag ttacctgtaa ttagatacag agagagattt tggcaataca
      181 tggagtaata caggatgtat tcaagtagca gaaaaaggtg cccgaaaacc aaatgggctt
      241 tgaaacttct tactgccgca tttttagcag cgagtcccgc ggcgaagagt gctgttaata
      301 acgcctatga tgcattgatt attgaagctc gcaagggtaa tactcagcca gctttgtcat
      361 ggtttgcact aaaatcagca ctcagcaata accaaattgc tgactggtta cagattgcct
      421 tatgggccgg gcaagataaa caggttatta ccgtttacaa ccgctaccgt catcagcaat
      481 taccagcgcg tggttatgca gctgtcgccg tcgcttatcg taacctgcaa caatggcaaa
      541 actcgcttac actgtggcaa aaggcgctct ctctggagcc gcaaaataag gattatcaac
      601 ggggacaaat tttaaccctg gcagatgctg gtcactatga tactgcgctg gttaaactta
      661 agcagcttaa ctctggagca ccggacaaag ccaatttact cgcagaagcc tatatctata
      721 aactggcggg gcgtcatcag gatgaattac gggcgatgac agagtcatta cctgaaaatg
      781 catctacgca acaatatccc acagaatacg tgcaggcatt acgtaataat caacttgctg
      841 ccgcgattga cgatgccaat ttaacgccag atattcgcgc tgatattcat gccgaactgg
      901 tcagactgtc gtttatgcct acgcgcagtg aaagtgaacg ttatgccatt gccgatcgcg
      961 ccctcgccca atacgctgca ttagaaattc tgtggcacga taacccagac cgtactgccc
     1021 agtaccagcg tattcaggtt gatcatcttg gcgcgttatt aactcgcgat cgttataaag
     1081 acgttatttc tcactatcag cgattaaaaa agacggggca aattattccg ccctgggggc
     1141 aatattgggt tgcatcggct tatctcaaag atcatcagcc gaaaaaagca cagtcaataa
     1201 tgaccgagct cttttatcac aaggagacca ttgccccgga tttatccgat gaagaacttg
     1261 cggatctctt ttacagccac ctggagagtg aaaattatcc gggcgcgcta actgtcaccc
     1321 aacataccat taatacttcg ccgcctttcc ttcggttaat gggcacgcct acgagcatcc
     1381 cgaatgatac ctggttacag gggcattcgt ttctctcaac cgtagcaaaa tatagtaatg
     1441 atcttcctca ggctgaaatg acagccagag agcttgctta taacgcacca ggaaatcagg
     1501 gactgcgcat tgattacgcg agtgtgttac aagcccgcgg ttggcctcgt gcagcagaaa
     1561 atgaattaaa aaaagcagaa gtgatcgagc cacgtaatat taatctggag gttgaacaag
     1621 cctggacagc attaacgtta caagaatggc agcaggcagc tgtcttaacg cacgatgttg
     1681 tcgaacgtga accgcaagat cccggcgttg tacgattaaa acgtgcggtt gatgtacata
     1741 atcttgcaga gcttcgtatc gctggctcaa caggaattga tgccgaaggc ccggatagtg
     1801 gtaaacatga tgtcgactta accaccatcg tttattcacc accgctgaag gataactggc
     1861 gcggttttgc tggattcggt tatgccgatg gacaatttag cgaaggaaaa gggattgttc
     1921 gcgactggct tgcgggtgtt gagtggcggt cacgtaatat ctggctcgag gcagagtacg
     1981 ctgaacgcgt tttcaatcat gagcataaac ccggcgcgcg cctgtctggc tggtatgatt
     2041 ttaatgataa ctggcgtatt ggttcgcaac tggaacgcct ctctcaccgc gttccattac
     2101 gggcaatgaa aaatggtgtt acaggcaaca gtgctcaggc ttatgttcgc tggtatcaaa
     2161 atgagcggcg taagtacggt gtctcctggg ctttcactga tttttccgac agtaaccagc
     2221 gtcatgaagt ctcacttgag ggtcaggaac gcatctggtc ttcaccatat ttgattgtcg
     2281 atttcctacc cagtctgtat tacgaacaaa atacagaaca cgatacccca tactacaacc
     2341 ctataaaaac gttcgatatt gttccggcat ttgaggcaag ccatttgtta tggcgaagct
     2401 atgaaaatag ctgggagcaa atattcagcg caggtgttgg tgcctcctgg caaaaacatt
     2461 atggcacgga tgtcgtcacc caactcggct acgggcaacg cattagctgg aatgacgtga
     2521 ttgatgctgg cgcaacgcta cgctgggaaa aacgacctta tgacggtgac agagaacaca
     2581 acttatacgt tgaattcgat atgacattca gattttaagg ataaatatgt tacgtaatgg
     2641 aaataaatat ctcctgatgc tggtgagtat aattatgctc accgcgtgca ttagccagtc
     2701 aagaacatca tttataccgc cacaggatcg cgaatcttta ctcgccgagc aaccgtggcc
     2761 gcataatggt tttgtagcga tttcatggca taacgttgaa gacgaagctg ccgaccagcg
     2821 ttttatgtca gtgcggacat cagcactgcg tgaacaattt gcctggctgc gcgagaacgg
     2881 ttatcaaccg gtcagtattg ctcaaattcg tgaagcacat cgaggaggaa aaccgctacc
     2941 ggaaaaagct gtagtgctga cttttgatga cggctaccag agtttttata cccgcgtctt
     3001 cccaattctt caggccttcc agtggcctgc tgtatgggcc cccgtcggca gttgggtcga
     3061 tacgccagcg gataaacaag taaaatttgg cgatgagttg gtcgatcgag aatattttgc
     3121 cacgtggcaa caagtgcgag aagttgcgcg ttcccggctc gttgagctcg cttctcatac
     3181 atggaattct cactacggta ttcaggctaa tgccaccggc agcttattgc ctgtatatgt
     3241 aaatcgtgca tattttactg accacgcacg gtatgaaacc gcagcagaat accgggaaag
     3301 aattcgtctg gatgctgtaa aaatgacgga atacctgcgt acaaaggttg aggtaaatcc
     3361 acacgttttt gtttggcctt atggcgaagc gaatggcata gcgatagagg aattaaaaaa
     3421 actcggttat gacatgttct tcacccttga atcaggtttg gcaaatgcgt cgcaattgga
     3481 ttccattccg cgggtattaa tcgccaataa tccctcatta aaagagtttg cccagcaaat
     3541 tattaccgta caggaaaaat caccacaacg gataatgcat atcgatcttg attacgttta
     3601 tgacgaaaac ctccagcaaa tggatcgcaa tattgatgtg ctaattcagc gggtgaaaga
     3661 tatgcaaata tcaaccgtgt atttgcaggc atttgctgat cccgatggtg atgggctggt
     3721 caaagaggtc tggtttccaa atcgtttgct accaatgaaa gcagatattt ttagtcgggt
     3781 tgcctggcaa ttacgtaccc gctcaggtgt aagcatctat gcgtggatgc cggtattaag
     3841 ctgggattta gatcccacat taacgcgagt aaaatactta ccaacagggg agaaaaaagc
     3901 acaaattcat cctgaacaat atcaccgtct ctctcctttc gatgacagag tcagagcaca
     3961 agttggcatg ttatatgaag atcttgccgg acatgctgct tttgatggca tattgttcca
     4021 cgatgatgct ttgctttcag attatgaaga tgccagtgca ccggctatca cggcttatca
     4081 gcaagcaggc tttagcggga gtctgagcga aattcgacaa aacccggagc aatttaaaca
     4141 gtgggcccgc tttaaaagtc gtgcgttaac tgacttcact ttagaactta gtgcgcgcgt
     4201 aaaagccatt cgcggtccac atattaaaac tgcacgaaat atttttgcac ttccggtaat
     4261 acaacctgaa agtgaagcct ggtttgcaca gaattatgct gatttcctaa aaagctatga
     4321 ctggaccgct attatggcta tgccttatct ggaaggtgtc gcagaaaaat cggctgacca
     4381 atggttaata caattgacca atcaaattaa aaacatccct caggctaaag acaaatctat
     4441 tttagaatta caggcacaaa actggcagaa aaatggtcag catcaggcta tttcttcgca
     4501 acaactcgct cactggatga gcctattaca actgaatgga gtgaaaaact atggttatta
     4561 tcccgacaat tttctgcata accaacctga aatagacctt attcgtcctg agttttcaac
     4621 agcctggtat ccgaaaaatg attaatcgca tcgtatcgtt ttttatatta tgtctggtgt
     4681 tatgcatacc cctatgcgta gcgtactttc actctggtga actgatgatg aggttcgttt
     4741 tcttctggcc gttttttatg tccattatgt ggattgttgg cggcgtctat ttctgggtct
     4801 atcgtgaacg ccactggccg tggggagaaa acgcaccagc tccccagttg aaagataatc
     4861 cgtctatctc cattatcatt ccctgtttta atgaggagaa aaacgttgag gaaaccatac
     4921 acgccgcttt agcacagcgt tatgagaaca ttgaagttat tgccgtaaat gacggttcaa
     4981 cagataaaac ccgtgccatc ctggatcgca tggctgcaca aattccccat ttgcgggtca
     5041 ttcatctggc gcaaaaccag gggaaagcca ttgcgcttaa aaccggagct gccgcggcga
     5101 aaagtgaata tctggtgtgc attgatggcg atgcgttatt agaccgcgat gcggcggcat
     5161 atattgtgga accgatgttg tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta
     5221 ttcgaacacg ttctaccctg gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg
     5281 gtttgatcaa gcgaacccag cgtatctatg gaaacgtatt taccgtttcc ggtgttattg
     5341 ccgcatttcg tcgcagcgcc ctggcagaag tgggttactg gagtgacgat atgatcaccg
     5401 aagatattga tattagctgg aagctgcagt tgaatcagtg gacgattttt tacgagccac
     5461 gggcgctgtg ctggatatta atgcctgaaa cgttaaaagg gctgtggaaa cagcgcctgc
     5521 gctgggctca gggcggtgca gaagtattcc tcaaaaatat gacaaggttg tggcgcaaag
     5581 aaaactttcg aatgtggccg ctgttttttg aatactgcct gacgacaata tgggccttca
     5641 cctgcctggt cggtttcatt atttacgcag tccaacttgc cggtgtaccg ttaaatattg
     5701 aattgacaca tatcgctgcg acacatactg ccggaatatt attgtgtacg ttatgtttac
     5761 tgcaatttat tgtcagcctg atgatcgaga atcgctatga gcataatctg acttcatcgc
     5821 ttttctggat tatttggttc ccggttattt tctggatgct gagcctggca acgacattgg
     5881 tatcatttac acgagtcatg ttgatgccta aaaagcaacg cgcccgttgg gtaagtcccg
     5941 atcgcgggat tctgagaggt taatatgaac aatttaatta ttacgacccg acaatcacca
     6001 gtacgtttac tggttgatta tgttgccaca accatcttgt ggacattatt tgcgttgttc
     6061 atattcttat tcgccatgga tctgctgacg ggttattact ggcaaagcga ggccagaagc
     6121 cgacttcagt tctatttttt gctggcagtg gcgaatgccg tcgtgttaat tgtctgggcg
     6181 ctgtacaata agctgcgttt tcaaaaacag cagcatcatg cagcctacca atatacgccg
     6241 caagaatatg cagagagctt agcaatacct gatgagctct atcagcaact acaaaaaagc
     6301 cacaggatga gcgtacactt caccagccag gggcaaataa aaatggttgt ttcagaaaaa
     6361 gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat
     6421 aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct
     6481 ctttaccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
     6541 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
     6601 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
     6661 gcgcctcgtc aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg
     6721 gcgcgatttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
     6781 agcgccggac cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
     6841 ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
     6901 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
     6961 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt
     7021 gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac
     7081 caatggcttc tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
     7141 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc acgacgagct ttgatcactg
     7201 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
     7261 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
     7321 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     7381 agagaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgca
     7441 agtggtggag cgtagcttaa caagacattg tccagaacag gacgttacca tccgcgatct
     7501 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataat tacaacagat
     7561 ctcg
//
LOCUS       DAPPNJ010000202         7465 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000202 DAPPNJ010000000
VERSION     DAPPNJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.204"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(93..916)
                     /locus_tag="RPM73_003782"
                     /pseudo
     CDS             complement(93..916)
                     /locus_tag="RPM73_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(970..2688)
                     /gene="proS"
                     /locus_tag="RPM73_003783"
     CDS             complement(970..2688)
                     /gene="proS"
                     /locus_tag="RPM73_003783"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDX6522090.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="RPM73_003784"
     CDS             complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="RPM73_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDX6522091.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="RPM73_003785"
     CDS             complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="RPM73_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDX6522092.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="RPM73_003786"
     CDS             complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="RPM73_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDX6522093.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4880..5533)
                     /gene="metI"
                     /locus_tag="RPM73_003787"
     CDS             complement(4880..5533)
                     /gene="metI"
                     /locus_tag="RPM73_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDX6522094.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5526..6557)
                     /gene="metN"
                     /locus_tag="RPM73_003788"
     CDS             complement(5526..6557)
                     /gene="metN"
                     /locus_tag="RPM73_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDX6522095.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6745..7317
                     /gene="gmhB"
                     /locus_tag="RPM73_003789"
     CDS             6745..7317
                     /gene="gmhB"
                     /locus_tag="RPM73_003789"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDX6522096.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1835 a         1932 c         1892 g         1806 t
ORIGIN      
        1 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
       61 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
      121 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
      181 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
      241 tgcgccagtg taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa
      301 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
      361 acgcactttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
      421 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
      481 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
      541 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
      601 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
      661 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
      721 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
      781 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
      841 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg
      901 cctttggttt atccatttta tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc
      961 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
     1021 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
     1081 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
     1141 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
     1201 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
     1261 cacctggaac ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc
     1321 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
     1381 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
     1441 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
     1501 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
     1561 atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
     1621 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
     1681 acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
     1741 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
     1801 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
     1861 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
     1921 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
     1981 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
     2041 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
     2101 ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
     2161 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
     2221 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
     2281 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
     2341 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
     2401 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
     2461 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
     2521 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
     2581 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
     2641 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
     2701 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
     2761 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
     2821 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
     2881 taggttttgc ccgtttcctc acctttacga taggccgggc gtgggtcctg cgccagtact
     2941 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
     3001 acctctgcgg taaaactcac cgccatctct gccgctggcg cactttgcgc atagctggca
     3061 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
     3121 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
     3181 tctttcagct ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgtgttgcg
     3241 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
     3301 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg
     3361 cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt
     3421 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
     3481 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
     3541 cagcctgacg atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg
     3601 cattggcctt cattttcgag gcgttgattt gcatccgctt acgcgcggtt ggaatgctcg
     3661 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
     3721 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
     3781 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
     3841 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
     3901 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
     3961 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
     4021 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
     4081 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
     4141 gcgttacgat caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg
     4201 ctggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
     4261 cgtcgtccag agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg
     4321 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
     4381 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
     4441 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
     4501 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
     4561 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
     4621 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
     4681 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
     4741 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
     4801 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
     4861 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
     4921 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
     4981 tatagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
     5041 cacccatcgc ggaatagccg accagggtaa tcagggtgat agttgccgca ttcaccagac
     5101 ccggcagtgc ttccggtaac agcaccttac ggacgatctg catcggcgtg gcacccattg
     5161 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
     5221 caataaacgg tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg
     5281 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
     5341 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
     5401 gccccgggcg cgtaacataa agcaggacgc cgaccggcag accaatcaca aagccaaaaa
     5461 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc aaccacatca
     5521 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
     5581 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg
     5641 ccaccggcgt aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
     5701 gtttcagaaa gcagtggggc atcgaccgac tgaccggtaa actccagacg cagcatcggc
     5761 acgcagtcag tgaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga
     5821 tgcagggtcg actgaataaa cttctgcgcc aacggcgttt tcggatgcga aaacacttca
     5881 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
     5941 cgctttacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
     6001 atgtctttca gcagctcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
     6061 tcatcacaca gcaatacttt gggattgctg gctaacgcac gggcaatcgc cacacgctgt
     6121 ttctgcccac cggaaagatt cgatgggtag ctatcatgct tatcgccaag accaaccaat
     6181 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
     6241 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
     6301 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg
     6361 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
     6421 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
     6481 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
     6541 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa
     6601 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
     6661 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
     6721 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
     6781 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
     6841 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
     6901 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
     6961 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
     7021 ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
     7081 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
     7141 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
     7201 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
     7261 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcataatga
     7321 ttaaaagatg agcaattgaa ataaaaatgc atttttccac ttgtcttcct gagacgactc
     7381 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
     7441 ggttgaagag aaaaatcctg aaatt
//
LOCUS       DAPPNJ010000203         7445 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000203 DAPPNJ010000000
VERSION     DAPPNJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.205"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            242..1678
                     /gene="aspA"
                     /locus_tag="RPM73_003790"
     CDS             242..1678
                     /gene="aspA"
                     /locus_tag="RPM73_003790"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDX6522097.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            1796..3097
                     /gene="dcuA"
                     /locus_tag="RPM73_003791"
     CDS             1796..3097
                     /gene="dcuA"
                     /locus_tag="RPM73_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDX6522098.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            3213..3551
                     /gene="cutA"
                     /locus_tag="RPM73_003792"
     CDS             3213..3551
                     /gene="cutA"
                     /locus_tag="RPM73_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDX6522099.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            3527..5224
                     /gene="dsbD"
                     /locus_tag="RPM73_003793"
     CDS             3527..5224
                     /gene="dsbD"
                     /locus_tag="RPM73_003793"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDX6522100.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            5261..5836
                     /locus_tag="RPM73_003794"
     CDS             5261..5836
                     /locus_tag="RPM73_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6522101.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            5943..6018
                     /locus_tag="RPM73_003795"
     tRNA            5943..6018
                     /locus_tag="RPM73_003795"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5976..5978,aa:Phe,seq:gaa)
     gene            6195..7385
                     /locus_tag="RPM73_003796"
     CDS             6195..7385
                     /locus_tag="RPM73_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX6522102.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
BASE COUNT         1839 a         1929 c         1853 g         1824 t
ORIGIN      
        1 gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt cacaaattaa
       61 taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa atcctcaagc
      121 agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga aaaacattcg
      181 aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa agaaggttca
      241 catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag ttccagctga
      301 tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca gcaacaacaa
      361 aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag ccgcagctat
      421 ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca ttgccgcatg
      481 tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg tctaccaggg
      541 cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata tcggtctgga
      601 actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc atgttaacaa
      661 atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt actcttccct
      721 gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta aagctgtcga
      781 attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac cgatgaccct
      841 cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa atatccaacg
      901 taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta ctggtctgaa
      961 cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca ctggcttccc
     1021 atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt atgttatggt
     1081 tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg acctgcgctt
     1141 gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac tgcaggcggg
     1201 ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta accaggtatg
     1261 cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag gtcagctgca
     1321 gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc acattctgac
     1381 caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta acaaagaagt
     1441 gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc cgttcatcgg
     1501 tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga gtgtacgtga
     1561 agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt tctccgtaca
     1621 gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg aacagtaatc
     1681 gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt cttgtgagca
     1741 gtaacttaaa aataacaacc taatatcaac ttgttaaaaa acaaggaagg ctaatatgct
     1801 agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat tggggggaat
     1861 aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg gcgttaaacc
     1921 cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg ctatttctgc
     1981 catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc tgctgcgccg
     2041 taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga ctatctttgc
     2101 tggtactggc aacatatctc tggcgacact gccagttatc gctgaagttg cgaaggaaca
     2161 aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta tccgcgcaga ttgcgatcac
     2221 cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag gtcatggcat
     2281 cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat
     2341 gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga tttatcgcaa
     2401 gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg
     2461 tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta tctatgcaat
     2521 catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca ccaacgcaat
     2581 cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag tcgataccga
     2641 caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt gtatcctggg
     2701 tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca aagataccgc
     2761 tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct ttgcttctgc
     2821 tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggctc tggcactgaa
     2881 cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc
     2941 gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacta cccgtatcgg
     3001 taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg ccctggccgt
     3061 ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga tcaatcgcgg ggcgttcacg
     3121 ccccgctttc tttcccgccg actaacatcc ttcccccgta cgttgtatag tgacctctct
     3181 cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg
     3241 cgtctgtcgt ggtgctatgt accgcaccag atgaagcgac agcccaggat ttagctgcca
     3301 aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct acctctctct
     3361 attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta aaaactaccg
     3421 tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat caaaccccgg
     3481 aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg ctcaacgcat
     3541 ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat tcgacgcgcc
     3601 gggacgttca cattttgtcc ccgcggatca agcctttgct tttgattttc agcaaaacca
     3661 acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc gtaaacagat
     3721 ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca
     3781 tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac
     3841 catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct gtgctgatgc
     3901 cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa
     3961 cgcagcgtca cagcctgtgt ctgtttcgca gcaagagcag cacaccgcgc aattgccctt
     4021 ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc
     4081 aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct ccactgccag
     4141 agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca cggcgctggg
     4201 tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc catacgtgct
     4261 cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct tgtttactct
     4321 acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc aacagggcgg
     4381 ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt caccatgcac
     4441 caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca tgtggctggg
     4501 cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc taattaccgt
     4561 ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt
     4621 tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg gtgatgtatg
     4681 gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct ttatcaccag
     4741 cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt
     4801 ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg cgcaaactca
     4861 gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga
     4921 agccaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg cctgtaaaga
     4981 gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag acacggtctt
     5041 acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa
     5101 tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc atccacaagc
     5161 acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg atcgccaacc
     5221 gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact
     5281 gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc tggagatggt
     5341 tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag acaaagaggc
     5401 aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc gtcgtcagct
     5461 gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc aggcgttatc
     5521 ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct gtacgtttta
     5581 tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg cggcctacga
     5641 tttcacccac gaactgttaa ccacactgga agttgacgat ccggcgatgg tagcaaagca
     5701 gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga
     5761 tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg
     5821 tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt
     5881 gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta atgcgccccg
     5941 ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc cttggttcga
     6001 ttccgagtcc gggcaccact ttcacttcca gattcatctc ccgaactcca cgaaactcaa
     6061 gtataatcac atcgttacaa gcaatctgaa catcctgaac tccaccgaac tctactgaac
     6121 tctctctcaa tcaagcgttc attgggggca ccggtggggg ctcattgtgt tcagtcagtt
     6181 tcaggagccc ccatatggca cttacagcca gacaggtcga aacagccaga cctaaagaaa
     6241 aagactataa actctctgac gaacgtggtt tatatctgct ggtaaaaacc acgggtgccc
     6301 gctactggcg gcttaaatac cggatagcag gaaaagagaa aaaactggcc ctcggcgtct
     6361 atcccgacgt ctcccttgct gaagccagaa tcaaacgcga cgatgcccga aaaattatct
     6421 ccgaaggtgg cgacccgggc gaaaagaagc gaaaggaaaa actcactcag aaaatctctg
     6481 ccaccaatac gttccatgcc cttgctacgg aatggcatca gcataaatct ttgtcatggt
     6541 ctgaaagtta cgccagaagc gtactggagg ctctggataa agatattttc ccgtatctgg
     6601 gcaaacgaag cgttactgat atcctcccgc tggaaatgct ggaaattctg cgccgcatag
     6661 aaaaacgtgg ctcgctggaa aaacttcgta aggtgcgtca atactgtaat cagatttttc
     6721 gttatgccat cgccaccgga cgagccactg tcaatccggc atctgaactg accagtacgc
     6781 tggcggcgcc aaaagctgca catttccccc acctgagagc agatgagctc cctgtttttc
     6841 tccggaagct cgctgaatat catggcagtc ctgttacccg catggcgaca aatctgctgc
     6901 ttctgacagg cctcagaacg attgaactac ggtccgctga atggtcagaa attgattttg
     6961 ataatgccct gtggacaatc cctgaaagcc gcatgaaaat gcgacgtaaa catgtcgtac
     7021 cactgtcacg acaggccact gacattctgc tgcagctcaa aactttctcc ggacaatacc
     7081 ggctggtttt cccgggacgt tgtgatatca acaagccaat gagcgaagcc agcatcaata
     7141 tggtgctcaa acgtatcggt tacgatggca gggcaaccgg tcatggtttt cgtcacacca
     7201 tgagtaccat tctgcacgaa cagggcttta attctgcctg gattgaaatg cagttagctc
     7261 atgtggataa aaacgccatc aggggtacct ataatcatgc ccagtatctc gatggtcgcc
     7321 gtgaaatgat gcaatggtac gcagattaca ttgattcgct ttccaggcaa gagagtcagg
     7381 gttaagaaca cttgttctga tactttctct caatccacaa gttctcctcc ggcctccagg
     7441 tccgg
//
LOCUS       DAPPNJ010000204         7389 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000204 DAPPNJ010000000
VERSION     DAPPNJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.206"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..324
                     /gene="ecpR"
                     /locus_tag="RPM73_003797"
                     /gene_synonym="matA"
     CDS             <1..324
                     /gene="ecpR"
                     /locus_tag="RPM73_003797"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDX6522103.1"
                     /translation="VVLIAARKSEALANYWYYNSNIRGVVYAGLSRDIRKELAYVING
                     RFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKI
                     YKLVQ"
     gene            399..986
                     /gene="ecpA"
                     /locus_tag="RPM73_003798"
                     /gene_synonym="matB"
     CDS             399..986
                     /gene="ecpA"
                     /locus_tag="RPM73_003798"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDX6522104.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1044..1712
                     /gene="ecpB"
                     /locus_tag="RPM73_003799"
                     /gene_synonym="yagY"
     CDS             1044..1712
                     /gene="ecpB"
                     /locus_tag="RPM73_003799"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDX6522105.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1738..4263
                     /gene="ecpC"
                     /locus_tag="RPM73_003800"
                     /gene_synonym="yagX"
     CDS             1738..4263
                     /gene="ecpC"
                     /locus_tag="RPM73_003800"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDX6522106.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4253..5896
                     /gene="ecpD"
                     /locus_tag="RPM73_003801"
     CDS             4253..5896
                     /gene="ecpD"
                     /locus_tag="RPM73_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDX6522107.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5865..6575
                     /gene="ecpE"
                     /locus_tag="RPM73_003802"
     CDS             5865..6575
                     /gene="ecpE"
                     /locus_tag="RPM73_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDX6522108.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6888..7217
                     /locus_tag="RPM73_003803"
     CDS             6888..7217
                     /locus_tag="RPM73_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDX6522109.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
BASE COUNT         1881 a         1937 c         1940 g         1631 t
ORIGIN      
        1 gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta ttacaatagc
       61 aatattaggg gcgtggtata cgctggactg agtcgtgata ttagaaaaga actggcctat
      121 gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac ggaccgggaa
      181 atggaaatta tccgcatgac ggcccaggga atgcaaccta aatcgattgc cagaattgaa
      241 aattgtagtg tgaagacagt gtatacccat cggcgtaatg ctgaggccaa gctgtactca
      301 aaaatatata agttggttca gtaaactcca ggcaagttag ttttaaaaaa tgactcactg
      361 ggacatcacg tcctcaattc aactcgggaa gaaatgcaat gaaaaaaaag gttctggcaa
      421 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
      481 aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaag ctggttgtga
      541 cgccactcgg tagcctggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
      601 aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt aaactgacct
      661 cacgtcttat caccaacaca ttaacccagt tggatacctc aggttccaca ctgaatgtgg
      721 gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
      781 ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat gccagcaatc
      841 gtaccaccgc acaggatggt ttcaccttca ccatcatcag cggcaccacc aatggtacca
      901 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
      961 agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt agcccccctg
     1021 ctttattcct ggacggaatt gttatgaaaa agcaccttct gcctctcgct ctgctgtttt
     1081 ccggaatatc tccggcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     1141 acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc cttatcaata
     1201 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatctca atggacaagc
     1261 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     1321 tccgcttctt ctataaggga cccgcagatg aaaaagagcg ctactaccgc attgtctggt
     1381 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     1441 cttccgcccg catcggcacc attctggtcg tcgccccccg ccaggcgaac taccactttc
     1501 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcctacg
     1561 gcccttgcct gaaagccgcc aacggtaagg agtgtaaaga gaattactac ctgatgccgg
     1621 gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa caaaggacgg gttgcacttt
     1681 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     1741 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     1801 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     1861 attccgcagg ccttcagtca ggcgcttcag gacggcatga gcgtcccgct ctatattcat
     1921 ctcgccggta gccagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggctg
     1981 gatgatggac agctacgcat ccggaaaata cagctggaag agagtgaaga taacgccagt
     2041 gtcagcgaac aaactcgaca gcagctgatg gctctggcca acgccccgtt caatgaggcc
     2101 cttaccatcc ccctgactga caacgcgcag ctggatctca gcttgcgcca actgctgctg
     2161 cagctggtgg tcaagcgcga agcgctgggc accgtactac gctcacgtag cgaagacatc
     2221 gggcagtcca gtgttaacac cctcagcagt aatctgagct ataacttggg cgtctataac
     2281 aaccagttgc gtaacggcgg gagcaacaca tccagctatc tgtcgctgaa taacgttact
     2341 gcgctgcgcg aacaccatgt ggtgctcgac ggctcgctgt acgggatcgg tagcggtcaa
     2401 caggacagtg aattatataa agcgatgtat gaacgcgatt ttgccggtca ccgatttgcc
     2461 ggtggaatgc tcgacacctg gaacttgcag tccttagggc cgatgaccgc catttcagca
     2521 gggaagattt acggcctttc ctggggaaac caggccagct ccaccatctt cgacagcagc
     2581 cagtcagcca cgccagtgat cgccttttta ccggcggcgg gcgaagtaca tctcacccgt
     2641 gatgggcggc tactaagcgt tcagaacttc actatgggca atcatgaagt ggatacccgg
     2701 ggtctaccgt acgggattta cgatgtggaa gttgaggtga tcgttaacgg tcgcgtgatc
     2761 agcaaacgcc cccagcgggt caataagctg tttagccggg ggcgcggcgt cggtgcacca
     2821 ctggcgtggc aggtatgggg cggtagcttt catatggatc gctggtcgga aaacgggaaa
     2881 aagacgcgac cagctaaaga gagttggcta gcaggtgcct cgacctccgg ctcattgagt
     2941 acgcttagct gggcggcaac gggatatgga tacgataatc aggcggtggg tgaaacccgt
     3001 ctgacgctgc cgcttggggg ggcgatcaac gttaacctgc aaaatatgct ggccagtgac
     3061 agctcatgga gcagcatcgg cagcatcagc gccactctac cgggaggctt tagttcgctg
     3121 tgggttaacc aggaaaaaac ccgcattggc aatcaattgc gacgtagcga tgccgacaac
     3181 cgtgcaatcg gcggcacact caacctgaac tcactgtggt cgaagctggg cacattcagc
     3241 atcagctaca atgatgaccg ccgttacaac agccattatt acacggcaga ttactatcaa
     3301 aatgtctaca gcggtacctt tggttcgctt ggcctgcggg ccggtattca gcgctataac
     3361 aacggcgaca gcaacgccaa tacagggaaa tatatcgctc tcgatctctc gctaccactg
     3421 ggcaactggt ttagcgcagg gatgacccat caaaacggct acaccatggc aaacctgtca
     3481 gcacgcaaac agtttgatga aggaaccatt cgcactgttg gtgccaatct gtcacgtgcc
     3541 atctccggcg ataccggtga tgacaaaacc ctcagcggtg gggcgtatgc acagttcgac
     3601 gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg cggacggcta catcaatacc
     3661 aacttgaccg ccaatggcag cgtcggctgg cagggtaaaa acatcgctgc cagcgggcgg
     3721 actgatggca acgctggggt gatattcaac accgggctgg aggacgacgg tcagatcagc
     3781 gccaaaatca acgggcggat tttcccgctt aacggcaagc gtaactatct cccgctctct
     3841 ccctatggaa gatatgaggt ggagttacag aacagcaaaa actcactcga cagctacgat
     3901 atcgtcagcg gccgcaaaag ccatctgact ctctatccag gcaatgtcgc tgtcattgag
     3961 ccagaggtga agcagatggt taccgtctcc ggtcgtatcc gtgcggaaga cggcacactg
     4021 ctggctaacg cacggattaa caaccatatc ggccgaaccc gaaccgatga aaacggcgag
     4081 tttgtcatgg acgtggataa gaaatatccc actatcgatt ttcgctacag tggcaataaa
     4141 acctgcgaag tggcactgga actcaaccag gcgcgcggtg ccgtctgggt cggtgatgtg
     4201 gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga caggagaaga gaatgagagt
     4261 taacctacta ataacgatga taattttcgc gctaatctgg ccagtaactg agctcagagc
     4321 ggcagtgagc aaaacaacct gggcggatgc accggcacgc gagtttgtgt ttgtcgaaaa
     4381 caactcagac gacaactttt tcgtcactcc tggcggggcg ctggatccgc gcctgaccgg
     4441 tgccaaccgc tggaccggtt taaaatacac tggttcagga accatctatc agcaaagcct
     4501 cggctacatt gataacggtt acaacaccgg cctttatacc aactggaagt ttgatatgtg
     4561 gctggaaaat tcaccagttt catctccttt aactggcttg cgctgcatca actggtacgc
     4621 tgggtgtaat atgaccacca gtcttatcct gccgcaaacc accgacgcca gtggatttta
     4681 tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac ggcatgttgt cagacgcgtt
     4741 ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt acaatgacca tcaatgcctg
     4801 ccagacctct gtgaactatg acgccagcag cggcgcacgc tgtaaggatc aggccaccgg
     4861 caactggtat gttcgcaacg tcacccatac gaaagcagca aatctgcggt taataaatac
     4921 ccactcgctg gcggaggtat ttatcaacag cgacggagta ccgactctgg gcgaagggaa
     4981 cgccgactgc cggacacaga ccatcggcag cctttcagga ttaagttgta agatggtcaa
     5041 ctataccctg caaacaaacg gactcagcaa cacctcaatc catatattcc cggcgatcgc
     5101 caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg cagttcagtc tgaatggcag
     5161 ttcatggaaa ccggtgagca ataccgccta ttactacacc ttcaacgaga tgaagagcgc
     5221 agactcgatc tatgttttct tctcgagcaa cttctttaag cagatggtga acctcgggat
     5281 cagcgatatc aacaccaaag atctattcaa ctttcgcttt cagaacacca catcaccgga
     5341 gtctggctgg tatgaatttt ctacctccaa cacgctgatt atcaaacccc gtgatttcag
     5401 catcagtatt atctccgatg aatatactca gacaccgtcg cgggagggat atgttggcag
     5461 cggcgagtcg gcactcgatt tcggctatat cgtaaccacc agcggtaaaa cagctgccga
     5521 cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt ggcgggcgct cctattgtgt
     5581 cttcagctcc gatgacggta aggcgaaagt accgttcccg gcgacacttt cctttattac
     5641 ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat gatagctggc gggatatgac
     5701 cgatgcgctg tggttgacca caccgtggac tgatatctct ggcgaagtgg ggcagatgga
     5761 taagaccaca gtcaaatttt cgattccaat ggataacgcc atttcgctgc gtacggtaga
     5821 tgataacggc tggtttggcg aagtcagcgc ttcaggagag attcatgttc aggcgacgtg
     5881 gcgtaacatt aactaaggcc ctgctgacag cggtctgtat gctggcggca cctttgacac
     5941 aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc cgagactgac tttgtcagca
     6001 aacgtgtagt gaataacaac aaaagcgcac ggatataccg tattgccatc agtgctattg
     6061 atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga tggtgaactg cttttcgccc
     6121 cccgccaact ggcgttgcag gctggtgaga gcgagtattt taaattttac tatcatggtc
     6181 cacgggataa ccgcgagcgc tactaccggg tctcatttcg cgaagtcccc actcgtaacc
     6241 agacaagacg tagcccaacc ggcggcgagg tcagcacgga gccggtggtg gtgatggata
     6301 ccattctggt agtacgacca cgtcaggttc agtttaaatg gtccttcgac aaggtgacag
     6361 gaacggtaag taacactggc aacacatggt ttaagctact gattaagcca ggatgtgatt
     6421 cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg agacgtggtt catcagcctg
     6481 agttacgtca gccggggaat cattatctgg tctataacga caaattcatt aagattagcg
     6541 attcctgtcc ggctaagccc ccttcggcgg actaagactt atccatcggt cgagggaaga
     6601 attccacctc agagctccaa aaatcgttta aatgatgtgg aagcgatcgt caatgtcacc
     6661 tgtaacgatg ggctttctca tatgcacacc atagtgttca ggagacacgg aaaggtatca
     6721 ggcagcctgc taatgagtgg tttaatcacg aattagtacg taaaatcggt aacggctgga
     6781 aatcattcaa tactcacact atcgaaagtt ccccagccaa ccgcggtacg ttcttacata
     6841 cgatgtaccg ctgttctctt tacgattttt agctgtactg gtgaattatg agcaatctga
     6901 atccatgcat gacgtgtggt gcctgttgtg catttttccg cgtctctttt tactgggccg
     6961 aagccgacga tgctggcggt actattcccg ccaggctcac tgaacaaata tccccttttc
     7021 accgatgcat gagcggtacc aatcagaaaa acccccgatg tattgccctt gcaggaaccc
     7081 cgggcaaaaa tgcctgctgc acgatatata aaaatcgatc gtccacatgc agagaatttg
     7141 ccatgtctgg tgagaacgga gtcgtcaatg aggcttgcaa tcgtgcaagg gctaaatacg
     7201 ggctgacacc gttataaaca tacaataatt aattgcactg ccccgccagc gataatagcg
     7261 gggcttcgtt tttcaggggt aacaaaaccc gatacttctt ctatttgcca gcaacaatgc
     7321 ctctcttctg tagcgttctt cggatctaca tcatcctgaa gtagcgcgat atactcactg
     7381 actatctga
//
LOCUS       DAPPNJ010000205         7347 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000205 DAPPNJ010000000
VERSION     DAPPNJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.207"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            92..610
                     /locus_tag="RPM73_003804"
     CDS             92..610
                     /locus_tag="RPM73_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDX6522110.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            820..2961
                     /locus_tag="RPM73_003805"
     CDS             820..2961
                     /locus_tag="RPM73_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX6522111.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3037..6798
                     /locus_tag="RPM73_003806"
                     /pseudo
     CDS             3037..6798
                     /locus_tag="RPM73_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1671 a         1920 c         2401 g         1355 t
ORIGIN      
        1 cggttccgtg aagtgagctg catctgcctg tctgggatta acggcaggtc attgtaagta
       61 gtaattaacc acgttaacaa ggagtttaca aatgccaaca ccgtgttata tctccatcac
      121 tggtcagacc cagggaaata ttactgcagg tgcatttacc gctgattctg tcggcaacat
      181 ctatgtgcag gggcacgaag acgagatgct ggtgcaggaa ttcctgcata acgttactgt
      241 cccgaccgat cctcagtccg gtcagcctgc gggtcagcgt gctcacaagc ctttcatttt
      301 taccgtggcg ctgaacaaag cggttccgct gatgtacaac gcactggcct ccggcgaaat
      361 gctgccaacc actgaacttc actggtggcg tacctctgtg gaaggcaaac aggagcatta
      421 tttcactacc cgcctgactg attcgacgat tgtcgatatg aaactgcata tgccgcattg
      481 ccaggacccg gcgaaacgcg agttcaccca gttgctcgaa gtttcactgg cctaccgcaa
      541 aattgaatgg gaacacgtca aatctggcac ctccggtgcc gacgactggc gcgcaccgct
      601 ggaagcataa gttaagccaa cagcatccgg ctatcaggcc ggatgctgtt tttgtattac
      661 gccgtagaaa gaaaggttgt cagtaagggg ttacgttcac gggagatggc ggctgccgga
      721 aacttcggcg gtagcgtgcc tgaacgcaag cgtaacactg tactgtcaca gccgctttaa
      781 cagcgaactg tctgtttttc cttaaccccg gaggttgcta tgtcaaccgg attacgtttc
      841 acgctggaag tggacggcct gccaccggat gcttttgcgg tggtctcctt tcatctgaac
      901 cagtcactct cttcgctttt ttccctcgat ctctctctgg tcagccagca gtttctctcc
      961 cttgaattcc agcagatcct cgacaaaatg gcctacctga cgatatggca gggcgatgac
     1021 gtacagcgcc gggtgaaagg tatggtgacc tggtttgaac tgggggagaa cgacaaaaac
     1081 cagatgctgt acagcatgaa ggtgtgccca ccgctgtggc gcacagggct gcgccagaac
     1141 ttccgtatct tccagaatga ggacatcgaa agcatcctcg gcacgatatt gcaggaaaac
     1201 ggggtgaccg agtggagccc gctgttcagc gagccacatc cttcccgtga gttttgtgtc
     1261 cagtacggtg agactgatta cgatttcctg tgccggatgg cggcggagga aggcatcttc
     1321 ttttatgagg agcacgcgca aaaaagtacc gaccagagcc tggtcctgtg cgataccgtg
     1381 cgttatctgc cggagtcctt tgagatcccc tggaatccga acacccgtac cgaggtgagc
     1441 accctctgca tcagccagtt tcgctacagc gcacaaatcc gcccttcttc cgtggtgacc
     1501 aaagactaca cctttaaacg acccggctgg gcagggcgtt ttgatcagga aggccagtac
     1561 caggattacc agcgcacaca gtatgaagtg tatgactacc ccggacgttt caagggtgcc
     1621 cacgggcaga actttgcccg ctggcagatg gatggctggc gcaacaacgc agaagtggcg
     1681 cgcggaacaa gccgttcgcc ggagatatgg ccgggacggc gaattgtgct gacggggcat
     1741 ccgcaggcga acctgaaccg ggaatggcag gtggtggcga gcgatcttca cggcgaacag
     1801 ccgcaggcgg taccgggacg caggggttca ggcaccacgc tggataacca ctttgcggta
     1861 atcccggcag accggacatg gcggccacag ccgttgctga aaccgctggt ggacggcccg
     1921 cagagcgccg tcgtgacggg accggcaggc gaggaaatct tctgtgatga acatggccgc
     1981 gtgcgggtga aatttaactg ggaccgctat aacccgtcaa accaggaaag ttcatgctgg
     2041 atccgtgtgg cacaggcgtg ggcaggcacc ggctttggta acctggcgat accgcgtgtg
     2101 ggtcaggagg tcattgtgga cttcctcaac ggcgatccgg accagccgat cattatgggg
     2161 cgtacctacc accaggaaaa ccgcaccccc ggcagcctgc cggggacgaa gacgcagatg
     2221 acgatccgct cgaaaaccta taagggcagc gggtttaatg aactgaagtt tgacgatgcg
     2281 acagggaaag aacaggtcta catccacgcg cagaagaaca tgaacaccga ggtgctgaat
     2341 aaccgcacca ctgatgtgat aaacaaccat gccgaaaaaa taggtaacaa ccaggcgatc
     2401 accgttacca ataaccagat ccagaacatt ggcgttaatc agatacagac ggttggtgtc
     2461 aaccaggtgg aaacggtggg cagtaaccag attatcaaag tgggatcaaa ccaggttgaa
     2521 aaggtgggga tcattcgtgc gctgacggtg ggtgtggctt accagacgac ggtaggcggc
     2581 attatgaata cctcggtggc gttgttgcag tcctcacagg tagggctgca taaatcactg
     2641 atggtgggaa tgggctacag cgtcaatgtg gggaataacg tcaccttctc ggtgggcaag
     2701 acaatgaagg aaaacaccgg acaaacagca gtttattctg ccggtgagca tcttgaactc
     2761 tgctgtggta aggcaaggct ggtgctgacg aaggacggaa gcatatttct caatggtacg
     2821 cacattcatc tggaagggga gtcggatgtg aacggtgatg cgccagtgat taactggaac
     2881 tgtggtgcca cacaacctgt accggatgcg cctgtgccga aagatttacc ccccggaatg
     2941 ccggatatgc ggcaattttg agtcaggtgc aaaaaattaa aacggaactg gtgcagtgac
     3001 cagataaacc atattaaaag cagaaggaat tgattcatgg gcggaaaacc ggcggcgcgg
     3061 cagggtgata tgacccgcaa gggactggat attgtgcagg gttcagcggg ggtgctgata
     3121 ggtgcgccga cgggagtggc ctgttcggta tgtccgggag ggattaccta tgctaacccg
     3181 gtgaacccgc tgctgggtgc gaaggtgctg ccgggcgaga cggaccttgc gctgcccggc
     3241 ccgctgccgt ttattctttc ccgcgcctac agcagctacc ggaccagaac acccgcgccg
     3301 gtgggggtgt ttggtcccgg ctggaaagcg cccttcgata tccgcttaca gatacgcgat
     3361 gaaggcctga tactcaacga caacggcggc aggagcattc actttgagcc gctgtttccc
     3421 ggcgagataa gctacagccg cagcgagtca ctctggctgg cgcggggcgg ggtggcggag
     3481 cagcacagtt cgcagccgct aagcgcactc tggcaggtgc tgccggaaga tgttcgcctg
     3541 agtccgcatg tgtacctggc gacaaacagc cttcaggggc cgtggtggat actcagctgg
     3601 ccggagcggg tgccgggggc ggacgaggtg ctgccgccgg agccgcccgc ataccgggtg
     3661 ctgacgggcg tggtggacgg cttcgggcgg acgctgactt tccaccgggc ggcggagggt
     3721 gacgtggcgg gcgcggtgac gggggtgacg gacggcgcgg gccgctgttt tcacctggtg
     3781 ctgaccacac aggcgcagcg ggcggaagcc ttccgtaaac agcgcgtcac gtctttatcc
     3841 tcccctgccg gtccccgttc tgtttcctct tctcaggttt tccccgacac gctgcccgcc
     3901 ggtacagagt acggtgccga taacggtatc cggctggagg cggtgtggct gacacacgac
     3961 ccggcctacc cggatgaaca gccgactgcg ccgctggcgc gctacacgta cacggccagc
     4021 ggagaactgc gggcggtgta tgaccgcagc gggacgcagg ttcgcgggtt tacttatgat
     4081 gcggagcacg ccgggcggat ggtggcacac cattatgcgg ggaggccgga gagccgctac
     4141 cggtatgatg ataccggtcg ggtgacggag caggtcaacc cggaggggct ggactaccgc
     4201 tttgagtacg gagagagccg ggtaatcatc acggacagcc tgaaccggcg ggaggtgctg
     4261 tacacggaag gcgagggtgg cctgaagcgg gtggtgaaga aggagcatgc cgacgggagc
     4321 atcacccgca gcgagtatga tgaggcgggg aggctgaagg cgcagacgga tgcggcgggg
     4381 cggcggacgg agtaccggct gcatatggcg tctggaaagc tgacttcagt gatactgccg
     4441 gacggcagga cggtgcggta tggctataac agccagttgc aactgacttc agtgacatat
     4501 ccggatggtt tacgcagcag ccggaaatat gaccgtcagg gccgactggc agaagagatc
     4561 tcgcgcaacg gtaacattac gcgctggttt tatgattctt cccgcagtgg cctgccctgt
     4621 gcggtggaag acggaaccgg ggtgaggcgg agaataacgc gaaaccggta cggtcagctt
     4681 caggccttta cggactgctc ggggtacgcg acgcggtatg aatatgaccg gtacggtcaa
     4741 caaatcgcca ttcaccggga agaaggcatc agcacttaca gcagttataa tccgcgtggc
     4801 caactggtca gtcagaagga tgcgcagggg cgtgaaaccc gttatgagta cagcgccgca
     4861 ggcgacctga cagcgacgat atcaccggac ggtaaacgca gcaccatcga atatgataaa
     4921 cgcgggcgtc cggtgagtgt gacggaaggc ggactgacgc gcagcatggg gtatgacgcc
     4981 gccgggcgca tcaccacgct gactaatgag aacggcagcc agtccacgtt cctgtatgac
     5041 ccggtggaca ggctgacgga gcagcgcggc tttgatggca ggacgcaacg ttaccagtat
     5101 gacctgaccg gaaaactcac gcagagtgaa gacgagggac ttatcaccct ctggcactac
     5161 gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg acccggcgga gcagtggcag
     5221 tatgatgagc acgggtggtt aaccaccatc agccatacca gtgaaggcca ccgcgtggcg
     5281 gtccactacg gctatgacga taaaggccgc ctgacgggcg aacggcagac ggtggagaac
     5341 ccggagacgg gggagatact gtgggagcat gagacggggc atgcatacag cgaacagggg
     5401 ctggcgaccc gtcaggagcc ggacggcctg ccgccggtgg agtggctgac gtatggcagc
     5461 ggttatcttg cggggatgaa gctgggcgga acgccactgg tcgagtacac gcgggaccgg
     5521 ctgcaccggg agacggtgcg cagcttcggc ggggaggcct gtgaacttgc caccgcctgg
     5581 aataccagcg gccagctaca gagcaggcac ctgaaccttc cgcagcttga ctgtgactat
     5641 acgtggaacg ataacggaca actaatccgc atcagcggcc cgcaggagag ccgggagtac
     5701 cgttacagcg acacgggaag gctgacgggc gtccacacca cggcggcgaa cctggatatc
     5761 gatatcccgt atgcgacgga cccggcagga aaccggctgc cggacccgga actgcacccg
     5821 gacagcaccc tcacggcatg gccggataac cgcatcgcgg aagatgcgca ctatgtctac
     5881 cgccacgatg aatacggcag gctggcggag aagacggacc gcatcccgga aggggtgata
     5941 cggatgcacg acgagcggac ccaccactac cactacgaca gccagcaccg cctggtgttc
     6001 tacacgcgga tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg
     6061 ggacggcgaa tggcgaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg
     6121 ctgtcgcgta aaccggaggt gacatggtac gggtgggacg gtgacaggct gacgacgata
     6181 cagaccggca ccacgcgtat ccagacggtg taccagccgg ggagcttcac gccgctcctg
     6241 agaatcgaaa cagagaatgg cgaacaggcg aaggcgcggc accgtagcct ggcggaggtg
     6301 ttgcaggagg acacgggtgt gacgctaccg gcggagctgg cggtgatgct ggggaggctg
     6361 gagcgggaac tgcgcgcggg agcggtgagt gcggagagtg aggcatggct tgcgcagtgc
     6421 gggctgacgg cggaacagat ggccgcgcag atggaggcgg aatacatccc ggagaggaaa
     6481 cttcatcttt accactgtga ccaccgggga ctgccgcagg cgcttatcag cccggaaggg
     6541 gaaacggcgt ggtgcgggga gtatgacgaa tggggaaacc tgctgggcga aaccagtgcg
     6601 cagcaccttc aacagtcact ccgtctgccg gggcagcagt atgatgagga gtcggggctg
     6661 tactataacc ggaaccggta ctacgatccg ttgcagggac gctatatcac ccaggatccc
     6721 attgggctga aggggggatg gaacctgtat gcgtatccgc tgaacccggt gcagcggggg
     6781 gatcctcttg ggttgtctgg tgagacactg gttcttggtt ctgccacaac agtaacagga
     6841 acaaaatttg ggggggcgtt actttcgggt ggggcggcga cttttgatgc agtcacgacg
     6901 gctatggcgt ctgctgcccc tgctcttgga gcaatggctg tggtcgctgg gccaatggga
     6961 tatgaccttc tcgctaatga tgggcgtgcc gggaaggctc tgagcgatgg gcagttaata
     7021 aattctcagg ctgaaatgtg ttactggacc agtagttgtc ctttagatag cccacatgag
     7081 aatgataatt cgtcagggag tcagccgaat gttgggaagg atttgacgga tgaagagaag
     7141 aactgcctgg ccggcggcag tgcaggaagt ccgggaggat ggggtccaga ggatgaggaa
     7201 aaggctcgca gtcgggggcg tggttctgat acagaagcaa aaataaagga tctggatctt
     7261 cttcattcgc cggaaactat tggctcgcgt cctgatcttg ctaaactaac agacagtgag
     7321 ttgttagagt ctgttattaa tcctaag
//
LOCUS       DAPPNJ010000206         7225 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000206 DAPPNJ010000000
VERSION     DAPPNJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.208"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(296..1432)
                     /locus_tag="RPM73_003807"
     CDS             complement(296..1432)
                     /locus_tag="RPM73_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX6522112.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1435..1797)
                     /locus_tag="RPM73_003808"
     CDS             complement(1435..1797)
                     /locus_tag="RPM73_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDX6522113.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2334..4247
                     /gene="mtlA"
                     /locus_tag="RPM73_003809"
     CDS             2334..4247
                     /gene="mtlA"
                     /locus_tag="RPM73_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDX6522114.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4477..5625
                     /gene="mtlD"
                     /locus_tag="RPM73_003810"
     CDS             4477..5625
                     /gene="mtlD"
                     /locus_tag="RPM73_003810"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDX6522115.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5625..6212
                     /gene="mtlR"
                     /locus_tag="RPM73_003811"
     CDS             5625..6212
                     /gene="mtlR"
                     /locus_tag="RPM73_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDX6522116.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6224..6433)
                     /locus_tag="RPM73_003812"
     CDS             complement(6224..6433)
                     /locus_tag="RPM73_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522117.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6718..7080
                     /locus_tag="RPM73_003813"
     CDS             6718..7080
                     /locus_tag="RPM73_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDX6522118.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1828 a         1824 c         1838 g         1735 t
ORIGIN      
        1 gactgtcatc cttcagataa taaagccatc atcgcaatag atggaaaagc actcctgctc
       61 ttttttgatt agagccgtcg caggaggaac aactcatatc atcattgcga tctcgacgat
      121 gtatagtctc atcctgcggc agaacaagac ggataaaaaa tccaatgaga gcacactcat
      181 ccagaactta ttaacataat gaatattaaa gaaaaatcat cgcagctaat gaagtaatat
      241 gcaaaaataa ttagtcctta caaggagcaa agttccataa tctcccccct ccccctcaat
      301 gatccaaata aagataatgc atccagctgg tcattcttag cagcactttc cgcatcaccg
      361 aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg
      421 catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc gtgccgggca
      481 cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc acgacaggta
      541 aaatactagt cagtttgccg tgaaacacct gcccaggtag cgcgttaaac accacttccg
      601 catcatcgcc gggtttcaga cgtaacagcg agttttgccg aaattgggcg acaatttgcc
      661 gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct gcgtatgtac
      721 ctgggcggat cagtacctga gtaacgtagc cattgctcgg cgcgcgaatg acagtctgct
      781 caaggttata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc gattgctcgc
      841 cgttaaccat actgtcgagc tggctctgga tctgcgcctg ctccgccacc gagcctttca
      901 ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg
      961 gattcaccgc cgcctggctg cctttcaagt aacgttgata atttttaaac agacggtcgc
     1021 gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca cgcagcgtct
     1081 ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc gcctggtaac
     1141 gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta ttcttgtcag
     1201 tgacttcagt aacaattccc gtcacctgtg gcgtgatggg gatcgctatc actgcctttt
     1261 gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa
     1321 acacgccacc cagcgccgcc gtcgccagcg tccactgatt caccggaata cgaaagattt
     1381 taaagaccgc ccacgccagc gccacgtaag ttaaaacaat caatagatcc ataattagat
     1441 ctccgggaac gtggaatagt cagtcttttt ctcggcggcg agttgatgct ccagccggga
     1501 aattcggtca gaaagtgcgg cgatttccgg gtcagtcgct ttctcctgcg acgcaacatg
     1561 tgactgcata ccccagccac gctccggttg atagagcgtc gcccagatcc acagaaacgg
     1621 ccagataaca tgcagagtaa acaggctcac ccagcccgcc gtatgaatag cgtcggcatg
     1681 gggatggttg cgctttttgg caatcagata agggatgtca tgtatcgcga tgattccata
     1741 aaaaatcacc agaaagacga agatcagcac tcccaacgcg aaatagttta gaaacatatc
     1801 tgcctcggat tcacgtttat cagtgttgtt tttgggctgg cagccagaag ggagtcaggc
     1861 tgatattttg acaataatcc gggttcgcga ttctcgccat aacaccaaag aataattttt
     1921 agaggtgatg agttgcttag ttacataacg attgtatgac gaaggcataa catgctgtag
     1981 atcacatcag gtgaacgccg taagaaaata tcttgtgatt cagatcacaa agattcaaca
     2041 aaccatcaaa acaaaaatgt gacactactc acatttaaat gccattttta gcgaaaatcg
     2101 ccgtcttgtt gcttttttac acaagcgttt tgtgatgagt atcacgtcaa ttacctctct
     2161 accccctata tttatgtgac tgatatcaca caaaaggccg tcgactggac agttaaccga
     2221 ttcagtgcca gatttcgcag tatctacaag gtccggctac ctctgccgcc acattaacaa
     2281 aaaacctcgg gcttccagcc tgcgcgacag caaacataag aaggggtgtt tttatgtcat
     2341 ccgatattaa gatcaaagtg caaagctttg gtcgtttcct cagcaacatg gtgatgccaa
     2401 atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt atttattcca acagggtggt
     2461 taccgaacga gacgctggcg aagctggtcg ggccgatgat cacttatctc ctgccgctgc
     2521 tgatcggtta taccggtggt aagctggtag gcggcgaacg tggcggcgta gtcggtgcca
     2581 tcactaccat gggcgttatc gtcggcgcag acatgccgat gttcctcggt tctatgattg
     2641 caggtccgct gggcggctgg tgcattaagc acttcgaccg ctgggtagac ggtaagatca
     2701 aatccggttt tgagatgctg gtgaataact tctccgcagg catcatcggg atgatcctcg
     2761 ccattctggc atttctcggc attggcccga ttgttgaagc cctgtccaaa atgctggctg
     2821 cgggcgttaa cttcatggtt gtccatgaca tgctgccgct ggcgtctatc tttgttgaac
     2881 cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg tatcttctcg ccgctgggta
     2941 ttcagcagtc ccatgaactg ggtaaatcca tcttcttcct gattgaagct aacccaggtc
     3001 caggtatggg cgtgctgctg gcgtacatgt tctttggtcg tggtagtgct aaacagtctg
     3061 cgggcggtgc ggcaatcatc cacttcctgg ggggtatcca cgaaatctac ttcccgtatg
     3121 tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg cggtatgact ggcgtgttca
     3181 cgctgactat cctgggcggt ggtctggttt ctccggcatc tccgggttct atccttgctg
     3241 tactggcgat gacaccaaaa ggtgcttatt tcgctaacat cgcgggtgtg tgtgcggcga
     3301 tggctgtctc cttcgttgtc tctgctattt tgctgaaaac cagcaaagtg aaagaagaag
     3361 atgatattga agcagcaact cgtcgtatgc aggacatgaa agctgagtct aaaggcgcat
     3421 ctccgctgtc tgctggcgat gtgactaacg acctgagcca cgtacgtaaa atcatcgttg
     3481 cctgtgacgc cggtatgggt tccagtgcga tgggcgcagg cgttctgcgt aagaaaattc
     3541 aggatgcagg tctgtcgcag atttctgtta ctaacagcgc gatcaacaac ctgccgccag
     3601 atgtggacct cgtcatcact caccgtgacc tgaccgaacg cgctatgcgc caggttccgc
     3661 aggcacagca tatttcgctg accaacttcc tcgacagcgg cctgtacacc agcctgaccg
     3721 aacgtctggt tgctgcccaa cgccacacgg caaacgaaga gaaagtaaaa gacagcctga
     3781 aagacagctt tgacgattcc agtgctaacc tgttcaagct aggcgcggag aacatcttcc
     3841 tcggtcgcaa agcggcaacc aaagaagaag cgattcgttt tgctggcgag cagctggtga
     3901 aaggcggtta cgttgagccg gaatacgttc aggcgatgct ggatcgtgaa aaactgaccc
     3961 cgacttatct gggtgagtct atcgcggtgc cacacggtac ggttgaagcg aaagatcgcg
     4021 tactgaaaac gggcgtggtg ttctgccagt acccggaagg cgtgcgcttc ggtgaagaag
     4081 aagatgacat tgcccgtctg gtgattggta ttgcagcccg taacaacgag cacattcagg
     4141 ttatcaccag cctgaccaat gcactggatg atgagtccgt catcgagcgt ctggcacaca
     4201 ccaccagcgt ggatgaagtg ctggaactgt tggcaggtcg taagtaatcc aatcccaccc
     4261 tctccacatg gagaaggtgg ggttaattgc ctgatgcgct acgcttatca ggcctacagg
     4321 atgcatcacg atttgttgaa tttgcacgtt cttgtaggcc ggataaggcg cttacgccgc
     4381 atccggcgct gcccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag
     4441 ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca
     4501 ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg
     4561 tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt
     4621 catgtggttg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc
     4681 attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc
     4741 ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa
     4801 caaggtaatg aatccccgtt gaacatcatc gcctgtgaaa acatggtacg cggtaccacg
     4861 cagctgaaag gccatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagaa
     4921 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
     4981 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
     5041 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
     5101 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
     5161 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
     5221 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
     5281 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
     5341 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
     5401 aagccattgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
     5461 gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact ggcagcactg
     5521 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc
     5581 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
     5641 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
     5701 aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
     5761 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
     5821 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
     5881 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
     5941 aatacgaaga tgcggagctg ctgatggcat tgcgtgaaga actaaatcac gacggcaacg
     6001 agtacgcctt taccgacgac gagatccttg gaccctttgg tgaactgcac tgcgtggcgg
     6061 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
     6121 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
     6181 gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
     6241 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
     6301 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
     6361 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
     6421 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
     6481 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
     6541 atccgctgct gtttattatc ttttttctat agatttctta attaatcaat aaattgcgta
     6601 gtttttaagc atgttaatga ttacgttaca gaactattgc aatgtcatca gttgtgaaca
     6661 aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag ccatcacatg
     6721 aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc gtctggacgc catccgccac
     6781 cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct ggaaaaagag
     6841 aaagccacgc tggaagcaga aattgctcgc ctgcgtgaag ttcatagcca aaaactgagt
     6901 aaagaagcac agaagctgat gaagatgccg tttcagcgcg caattaccaa aaaagagcag
     6961 gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc
     7021 gcgctgggcc gtgaaatggg cctgcaggag atgactggat tctctaagac tgcgttttaa
     7081 gcgcctcaga cgactggttc gatcatatca acccgcatct ggcttgcctt agctggtcaa
     7141 tgcggggact ctcctgtcta ctcgcctaat taccaaacaa ttcactccct ttacttcgat
     7201 ttaccgctgg ttgatattct gaaaa
//
LOCUS       DAPPNJ010000207         7224 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000207 DAPPNJ010000000
VERSION     DAPPNJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.209"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            198..901
                     /locus_tag="RPM73_003814"
                     /pseudo
     CDS             198..901
                     /locus_tag="RPM73_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            complement(1108..1554)
                     /locus_tag="RPM73_003815"
     CDS             complement(1108..1554)
                     /locus_tag="RPM73_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDX6522119.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1646..2305)
                     /locus_tag="RPM73_003816"
     CDS             complement(1646..2305)
                     /locus_tag="RPM73_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDX6522120.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2377..2670)
                     /locus_tag="RPM73_003817"
     CDS             complement(2377..2670)
                     /locus_tag="RPM73_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDX6522121.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2912..3313
                     /locus_tag="RPM73_003818"
     CDS             2912..3313
                     /locus_tag="RPM73_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDX6522122.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3433..3801)
                     /locus_tag="RPM73_003819"
     CDS             complement(3433..3801)
                     /locus_tag="RPM73_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522123.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4321..5016
                     /gene="minC"
                     /locus_tag="RPM73_003820"
     CDS             4321..5016
                     /gene="minC"
                     /locus_tag="RPM73_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDX6522124.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5040..5852
                     /gene="minD"
                     /locus_tag="RPM73_003821"
     CDS             5040..5852
                     /gene="minD"
                     /locus_tag="RPM73_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDX6522125.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5856..6122
                     /gene="minE"
                     /locus_tag="RPM73_003822"
     CDS             5856..6122
                     /gene="minE"
                     /locus_tag="RPM73_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDX6522126.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6494..6838)
                     /locus_tag="RPM73_003823"
                     /pseudo
     CDS             complement(6494..6838)
                     /locus_tag="RPM73_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6873..6992)
                     /locus_tag="RPM73_003824"
     CDS             complement(6873..6992)
                     /locus_tag="RPM73_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDX6522127.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6953..7138)
                     /locus_tag="RPM73_003825"
     CDS             complement(6953..7138)
                     /locus_tag="RPM73_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522128.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
BASE COUNT         2074 a         1712 c         1584 g         1854 t
ORIGIN      
        1 tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaactgca gatggagcat
       61 tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca
      121 taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagtcggcg
      181 atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt
      241 atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tatttttcta tttgatgagt
      301 acaatgagaa gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca
      361 tcacgaagct gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg
      421 cttccgggaa actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca
      481 gcttttccag tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg
      541 tgtcgtcgcc ggtccatttg gattaatcat ttcctattct attgctgcgg gcgtagttga
      601 agggaaactg attccagaat tgaagaacaa gttaaaatct gtgcagaatt tctttaccac
      661 cctgtctaac acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac
      721 caccgaaata gccgccatcg gggagataaa aacggaaact gaaacaacca gattctatgt
      781 tgattatgat gatttaatgc tttctttgct aaaagaagcg gcaaaaaaaa tgattaacac
      841 ctgtaatgag tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg
      901 ataagcgatt attctctcca tgtactcaag gtataaggtt tatcacattt aaaaacacag
      961 acgtttttta aagattgaga gttacctgtt gataataagg tatcgggtaa ctctcattat
     1021 gacagagcgc tttcaaaaac agggctacga aatggcgagc atccagacgt atattaagaa
     1081 agcccccatc aacatctatt aaatttatca ctgcgcccag tcaggtttat ttaagatatg
     1141 atcctgccag tcaatcactt ctgattcttt cactgcgata tgacgcacag agatacgttc
     1201 accatgcatt gccgctttcg aaccagtaag tagcggatgc cacgcaggta aatctttacc
     1261 ttccgccagc aaacgataag cgcaggtcat tggcagccat tcgaatgttg gcagattttc
     1321 acgggttaat ttaatgcagt cgggttcaaa ctcgaaacga cgttcgtagt tccgacattg
     1381 acaagtttta atattgagct ggcgacaggc gacgttagtg aagtagattt cgtcggtgtc
     1441 ttcatccatc agtttatgca ggcaacactg accgcaacca tcacacaacg actcccattc
     1501 cgcatcgctc atttcgtcca gggttttact ttgccagaaa ggtacatcgc tcatcaggtg
     1561 ttccgccatt acgttaaaac gcaccttata accagtctgg cacagcgatg caagttttgc
     1621 cgccgctttc aggcggcaaa aagtattaca aaacgcgagt tgtcaaagaa tgcccatcga
     1681 aagtgactgt cagctcatca ccgctttgca acgggccgac gccatcaggc gtgcctgtca
     1741 acacaacgtc accggccttg agggtaaaaa acttgctcat ataagcgatc aacggaacga
     1801 ttttatggat catgtccgca gtcgtacctt gctggcgttg ttcgccgttt acgctcaggc
     1861 ccagcgttgt attttgtgga tcgccggtaa attccgccgc ggggataaac ccggaaagcg
     1921 gacaagagtt atcaaacgct ttagcctttt cccacggctg cccggctttc ttcatttttc
     1981 cctgaacatc acgcaatgtc agatcaagcg ccacgccgta accggcaatg gctttgcgga
     2041 catgctcttc cgttgcctga cgcagcgtcg cgccaatcaa caccgccagt tcgacttcat
     2101 gatgaactga accgaaatcg gatgggatcg ccaatggctg ccgcagatcg cacagtgccg
     2161 tttctggttt aataaacagc actggctctt cgggcactgc gctgcccatc tctttaatat
     2221 gtttggcata gttactgcca acacagacta ctttactcac cggataatcc agcagcgcac
     2281 cttgccagtt gtgatgttga tacataacgt tcccctcacc ttatcgtgga tatgaaaacc
     2341 cgcgccgaca aagataatgc caccgccgga tatcggttat ttgttagtgt cgtctgtttt
     2401 ctgccccatg acggaaagat gttgcttcag caaatcttcg ggtggtggcg gtaactgcaa
     2461 atagtaacct tgctcggtta atgccagttt aaccttttca atatcagcat tgaccagttt
     2521 cttgcgccca tccagcggca gaatcatcgc taactgaggc tgaccaaaac ctttcatcag
     2581 ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca tataaatagg tctggtcacg
     2641 cttgctgctt cgataaatca cacaaaacat acttttactc tttaagatgc caggttttgg
     2701 cataagctaa catcaatgaa acccgcgcca caagccagcc agctgtagat ttctgcggtt
     2761 tgttgagtcc atgccccttt ataagtaatc cccccggcaa aaaatgccgc gccgggattt
     2821 taccgaacgc agaaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt
     2881 catcgtactc attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt
     2941 attgctgctt gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga
     3001 gttcagcaaa ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga
     3061 tacatatacc ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg
     3121 cgctgatact tacctcttcg ggccaggaat cgatgattca gttgatttat ccagatattc
     3181 accagaactc gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg
     3241 agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat
     3301 tcaaattaag taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac
     3361 tcctctctgt cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat
     3421 gtaaacaagg catcactcac gacacataaa taacatcttt gtggtgattt gattgatttt
     3481 tccactccgc ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac
     3541 atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg
     3601 taagttttca gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc
     3661 tgagttcaca caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc
     3721 agcgtcatgt ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata
     3781 aaaaatacgc atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc
     3841 atttcatcat caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag
     3901 aagtatcaga cgtcttctgc gttttctatg atagagcaat tctgtggcgg atattaagat
     3961 aacgggtatc tgttctttaa tgatgccgca aaaaatggaa agttaagaaa gcctggcgtg
     4021 gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga tgctgtcacg
     4081 attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt
     4141 tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt
     4201 tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta
     4261 tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg
     4321 atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg
     4381 catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc
     4441 gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac
     4501 tggtcagcga tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc
     4561 tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat cctgacggaa
     4621 ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg ctccagcgca aaatacaacg
     4681 ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct
     4741 ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt gattgccgat
     4801 gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc aagtggtgac
     4861 cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa
     4921 tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta
     4981 gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta
     5041 tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg
     5101 cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata
     5161 tcggcctgcg taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt tacgatttcg
     5221 tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg
     5281 aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag
     5341 gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact
     5401 ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta
     5461 ttaccaccaa cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg
     5521 cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa
     5581 cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg
     5641 agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct
     5701 ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag
     5761 ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga
     5821 aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct
     5881 cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac
     5941 gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg
     6001 tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag
     6061 atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa gagctgaaat
     6121 aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct tgagtttttc
     6181 atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct
     6241 atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg
     6301 cctggttgag taaataaccc tgttgttagt atggatatac tgggagatga taaccccgca
     6361 atttcattat tcattgttcc cccataggat gaggtttccc gccgtaactg gcgggctttt
     6421 tttgccccag aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt
     6481 taatcgttga tggtcagaac gaccagttca caccagccac cgcgttccac ggggactcca
     6541 caccggcacc atggctatac cccaccccga aatgcccgct taacgtactg ctgaatgagg
     6601 ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt
     6661 cactattcac tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcaggc
     6721 agggttcaat ttccataccg ttccccagac gcatgttgta actcagcgtt gcacccagtt
     6781 cacgatatat actgcgggta tcgactgatt tcgattccat gccattggat aaatgatagt
     6841 agcgtctaag tgacctaagc tctgctgcaa catcagcccg caggcaccag accaggggat
     6901 tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga catcatgatg
     6961 aaatgatgat atcccgcata agaaagaggc attttttaca cgcagtgcgc tgaagtgtag
     7021 ttggataaaa aatcactctt ttcagataat actgtcgtac gcttatcttt cgacaatgag
     7081 gccgtcggca aaataaaatg actaatgtaa tcgttgctga tgaatatctt ctgctcacat
     7141 aaaaatcaca caataatatt gataaatcag attatcttac ctttacaaca ttgcgttaac
     7201 agtcaatgac acttcttgaa taaa
//
LOCUS       DAPPNJ010000208         7201 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000208 DAPPNJ010000000
VERSION     DAPPNJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.210"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            185..1120
                     /locus_tag="RPM73_003826"
     CDS             185..1120
                     /locus_tag="RPM73_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX6522129.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1176..1718)
                     /locus_tag="RPM73_003827"
     CDS             complement(1176..1718)
                     /locus_tag="RPM73_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX6522130.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1702..2013)
                     /locus_tag="RPM73_003828"
     CDS             complement(1702..2013)
                     /locus_tag="RPM73_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDX6522131.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2198..3088)
                     /gene="cyoE"
                     /locus_tag="RPM73_003829"
     CDS             complement(2198..3088)
                     /gene="cyoE"
                     /locus_tag="RPM73_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDX6522132.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3100..3429)
                     /locus_tag="RPM73_003830"
     CDS             complement(3100..3429)
                     /locus_tag="RPM73_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDX6522133.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3429..4043)
                     /locus_tag="RPM73_003831"
     CDS             complement(3429..4043)
                     /locus_tag="RPM73_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDX6522134.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4033..6024)
                     /gene="cyoB"
                     /locus_tag="RPM73_003832"
     CDS             complement(4033..6024)
                     /gene="cyoB"
                     /locus_tag="RPM73_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDX6522135.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6046..6993)
                     /gene="cyoA"
                     /locus_tag="RPM73_003833"
     CDS             complement(6046..6993)
                     /gene="cyoA"
                     /locus_tag="RPM73_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDX6522136.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1965 a         1778 c         1780 g         1678 t
ORIGIN      
        1 tttatcttac gaatatgcta gttttatctc actaaataaa cgatttggtg acatggatga
       61 taatgtttca ggctaaatgt gtgcttatta cttggacctt tatggcactg tatttattca
      121 ttaaatggat atttggtggt taattattat ggaaatatat gacagtgagg acataaaggt
      181 atacatgtta actaaaagaa tattattttc tgtaatgctg atagtttcac caagcgtggt
      241 ggccagcgag aaagcgcatg aattgtatga cagcatatac ggtggtaaac ctgctcccga
      301 tgttataaat acactgcata aaatggctga atcgggagat atcgacgcgc agagcctgtt
      361 aggctgggaa tattatcagc ctcgttatga taccaaacct gatgttcagg aagcgattaa
      421 atggttcgag ttagcggcta aacaaggtga cagagaagct ccgttagcgc tggggggtat
      481 ctactacgac ggtgagcagg tgcgggtgga ttatgccaaa gcgtatgcac tatttaatca
      541 ggccgcgcag cacggtgtga atttagcatg gtccaggttg ggcattatgt acgccaatgg
      601 tcagtatgtt gaggtagatt gtaagaaagc gaaggaatat cttgataaag gtgtccacat
      661 ttatggtggc ccggaagact ttctggctac ttgtcgaaaa gacatgattg acagaaaaac
      721 ggttgacgat acgttacccg tgattacggt tacccgttct gggatgcgag ataatttttt
      781 agataaaggt ttttcctgca tggatagcct cttcgccacc accaataaat taggcgaagt
      841 ggccaatctt cgcgtgacat tcagcattcg tcgcccatcc ggtaaggaga ttaaccaaac
      901 ggttggcttc gcgcctttcg gtttaaaccg gctgaatatt agctttactg attacctatt
      961 tggttcattt accagtaatt cgtctcttat tctttataaa ccggaatttg agcgaaaatc
     1021 ttgtgcaact gtaaggacca caatcgttgc cgcaacggca accattaacg gcaaggatgt
     1081 ggagttgctg aaagcggggg caattgaaca aaagtggtaa cgctgctcag cccccggctg
     1141 aggcaaaact tgccctggcg ggtggcgata ataatctaag ccgcgagaat atgctgaatc
     1201 gccaaaaaca taggtacagc accaaaagca tttggtgtcg ctgaaagctg aacaaagccg
     1261 agacgagcat aaaaattaat ggcggcaggg tcagcatcaa gataaacccc tttaattggt
     1321 aatgcctggt gaattatttt tacatgttca aaaaaatcac atagtaggtc ctgaccaaag
     1381 ccccgctttt gatacttccg tgctaccccc aacatgacta atctgacaac accaatttct
     1441 gaaggttgtg aaccctgaag gacgccagaa acgcgttgtt tttccagcga atatgccgta
     1501 aaagtacaga tgccgatcag ttcaccactt tgtctgtcaa taagtgcttt agccgcacag
     1561 tcgctattac gcacactttt ctttagcgat gcgcgtacaa atttatcgat aacagggtta
     1621 ccgcaatcaa actgtttttg cccaggataa gtaatatcag actgataatt acaggagagg
     1681 actatgggca gagtaatctc ttcatgcttg tcgaccatcg ctgtttttcc ttttcattaa
     1741 cgccttgagg gcgagcgtcg gttgagcagg ttcagcgatg agttggttca tcagttccca
     1801 gctttgattc gaaagctcac ggcgacgttg gttatcaagc acactttcgg cgcgttccat
     1861 agcagcgctc aaaataaaag cgcttaaatc taccccaacg gcttgcgcag cttcacgaag
     1921 tttgtttttg gcatcgggac tagttttgag ttcgacccgc gcatttttca gcgttttatc
     1981 gacatcgggt agagtggctg tttttccagg cattatgacc tctttgatta actgaatgaa
     2041 atcgtaggga gtcgatctca tacataatac ggctatcaac cgtatgatta atttatgttc
     2101 atctgctcac tctgtcaaga catacggtta ttagccgtat ctctgatctc agacaataaa
     2161 aaaggtgccg tagcaccttt ttaatagaga ggttttgtta ccacacagca gccagcagcg
     2221 tatgcgagtc cggtaccata aaatcaacgg acatcatcac cgagagggca gtgatggcga
     2281 tgatagagaa gccgaacagc ttgcgcgccc agattctgtc atcagcaact ttataaccgc
     2341 gcagagccat acctaaccac cagacgctaa ccgccgcggc gaccaccaga tatttatatc
     2401 cagcgtaacc gccaagagag agcatcagcg tggcaacggc aaaggcgatg atatacagcg
     2461 tgatgtgatt cttcgccacc gaaatgcctt ttaccactgg caataccgga atgtttgccg
     2521 cctggtaatc cttaaagcgg aaaatggcga tggcatagga gtgaggcatc tgccacaggc
     2581 tgaagatagc cagcaggatc gctgcgccgc tatcgaactc gccggttact gcacagtagc
     2641 caatcaccgg cggcgcagcg ccggagagcg aaccaatcaa tgtgccgtag actgagtggc
     2701 gtttcatgta caggctatag acgccgacat aaaccacaaa gcccatcacc cccagccagc
     2761 aggccagcgg attcgcgcca aaccacagca gcataaagcc agcaataccc agcaaggtgg
     2821 cgtacaccag cgagacagca ggagagatca ggcctttaac cagcacccga ttcttcgtcc
     2881 tttccatctt tctgtcgata tccctgtcga tgtagttgtt aaacacacaa cccgacgcca
     2941 caaccagtga caccccaacc agcgtgtaga taaacagggg ataatcaatg ctgccctttg
     3001 aggccagcag gaatccccca atcaccgaga tcaggttgcc aaagatgatg cctggtttcg
     3061 ttacttgcag gtattgctta aacatcataa ccgccgctct tagtgcatca tcatgttgta
     3121 gttgaggttc cacataatcc agatggagcc tacaaccagg atagcgatga ttagcacggt
     3181 gaagacaaac gccgtcatgt tccagccttc atctgatttg gtattcatgt gcaggaagca
     3241 caccagatgc accagaacct gtaccactgc cattgccagg attgttccca gaattacggc
     3301 cggagaggca gcccctgtca tcaccatcca gaacggaatc accgtcagaa tgatcgacag
     3361 gataaagcct gtcatgtagg tttttacgct gccatgggac gcgccgctgt gatcggtaga
     3421 atgactcatt acatcgcccc catcagataa acaacagtga acacacagat ccaaaccaca
     3481 tccaggaagt gccagaacag gctcaggcac atgatgcggg tacggttagt gctggtcagg
     3541 ccgcgacggg cgatttgcac catcagcacc gccatccaga taagaccaga agtgacgtgc
     3601 agaccgtgcg tgccgaccag cgcaaagaac gctgacagga agccgctgcg atccggaccc
     3661 atgccgttaa caatcaggtg atggaattca tagatttcca tcccgataaa tccggcacca
     3721 aacaaccagg tcaacgccag ccaggagata acctggcttt tgttgttttt gtacatggcg
     3781 atagccgcca tgccgtaggt gatggagctg aacaacagca agaaagtttc aaccagaacg
     3841 aacggcagtt cgaaaatgtc cttacctgtc gggccgcctg cggtgccgtt caccagaacg
     3901 gcataggtag caaacaagat tgagaacaga atgcagtcgc tcatcaggta gatccaaaat
     3961 ccgaagattt tggttccgcc tgcatcgtgg tgcccgtgtt cgtgcgcgtg ggcagtcgcg
     4021 tgcgtcaaag tatcagttgc catttttcag ccctgcctta gtaatctcat cgaaatgctg
     4081 gttttccagt ttttcgattt ctgccaccgg cacgtagtaa tccacgtcct cgtcgaagct
     4141 tttcacgatc caggtgatga tcatgcctgc gaagccaaca atcgccagcc accagatatg
     4201 ccagatcatg gcgaaaccga agatggtgga gaaagctgca atgacgatac ctgcaccgct
     4261 gtttttcggc atatgaattt cttcatagtg gtcaggcttt ttatacgctt cgcctttctc
     4321 tttcatttcc cagaatgcat cacgctcgtg aacgtgcggc acaacggcaa agttatagaa
     4381 cggaggcggg gaagaggttg cccactccag cgtacggcca ccccacgggt cgccagtcag
     4441 gtcacggttc tggtcgcggt cgcgaataga aacgtacatc tgaataacga ggcatagaat
     4501 acccagcgca atcagtactg caccgctagc tgcaatcatc agcatggtgt ggaactgcgg
     4561 gtcaatctgc tggctcaaac gacgggtcat gcccatgaag cccagcgcat acagcggcat
     4621 aaaggcaacg aagaagccga tgatccagaa ccagaacgcg cgtttacccc aggtttcatt
     4681 cagtttgaaa ccgaaggctt taggccacca gtaggtcatc cctgcgaagc agccgaagac
     4741 cacgccgccg atgatcacgt tatggaagtg cgcaatcagg aacaggctgt tatgcagaac
     4801 gaagtccgcg cccggtactg ccagcagcac gccagtcatc ccgcccaccg agaaagtgac
     4861 gataaaaccg atggtccaca gcatcgcaga atggaacacg atgcggccct gatacatggt
     4921 gaacagccag ttgaagatct tcaccccggt cgggatggcg ataatcattg tggtgatacc
     4981 aaagaaggcg tttacgttcg cgcccgcacc catcgtaaag aagtggtgca gccaaacgat
     5041 gaacgacagc acggtgatac agacggttgc ccataccagc gaggtataac caaacagacg
     5101 tttacgcgag aaggttgccg caatttcgga gaacacaccg aaaacaggca ggatcaggat
     5161 gtaaacttcc gggtggcccc aggcccaaat caggttgatg tacatcatca tgttgccacc
     5221 catatcgttg gtaaagaaat gggtgcccag atagcgatcc agggtcaaca acgcgacggt
     5281 aaccgtcaga attgggaagg aagcaataat cagtacgttc gcgcacagtg atgcccaggt
     5341 aaatactggc atcttgaaca tggtcatgcc cggtgcgcgc atcttcagaa tggtaacgaa
     5401 gaagttgata ccggtaagcg tcgtacctat accggatagc tggagactcc atatccagta
     5461 atcaacaccg actcccggac tgtactctat tcccgatagc ggtggatagg ccagccagcc
     5521 ggtctgcgca aattcgccca cgccgagaga aacgttaacc agaatcacac caacaacagt
     5581 aaaccagaag cttaagttgt tgaggaacgg gaacgcaacg tcacgcgcgc cgatctgcag
     5641 cggaaccacc aggttcatca gaccgataac gaaaggcatc gccacgaaga agatcataat
     5701 cacgccgtgc gcggtgaaga tctgatcgta gtggtgaggt ggcaggaagc ccgcttcacc
     5761 cgccgaggca agagcctgct ggctacgcat cataatggcg tcagcaaaac cacgcagcaa
     5821 catcacaatc gccacgatga tatacatgat accgaggcgt ttatggtcga cggaggtcag
     5881 ccactctttc cacaggtagg tccacttacc gaagtaagtg atcaggccaa cgagcgccag
     5941 acctcccaaa ataatgccag cgatcgtaac catgacgata ggttcatgga acgggactgc
     6001 atcaagtgat aattttccga acatctttat tcttcctcaa cccctttaat gggcggattc
     6061 cgcgtggctc atgtccatgc cttccatacc ttcgtgtgca ctgtgctcac cttctggctg
     6121 ggtcatgtcc atgctcttac cgtgagccat aaacttgtta atcacatcgg caaacaagtc
     6181 tggtttcacg ttggagaaat attccacctg gttgtattcg ctaggcgcgg ccagtttttc
     6241 gaacgcagcc atgtcagaca tggtgttcgg cgactgtttc gcttttgcga cccattggtc
     6301 gaattcggcg cgatccggtg ttgcaatagc tttgaacttc atgcctgaga agcccggccc
     6361 gctgtagctg gcggagatac cgtcataagt accgggttcg ttggcgatca gatgcaggcg
     6421 agtctgcata ccggccatgg cataaatctg gctacccaga cgcggaatga agaaggagtt
     6481 catcacggag ttggaggtca ctttgaagta caccggagtg ttcgccggga aagcgatttc
     6541 attcacggta gcaatgccct gttccgggta gatgaagaac catttccagt ccatggaaac
     6601 cacttcgatg gtaatgggct tctcgtcgtg tgccagcggc ttgctaggct caagagcgtg
     6661 agtggttttc caggtcagta ccgcaaggaa gatgatgatt aagataggta ccgtccagac
     6721 cacagcttcc actttattgg agtgtgacca gttcgggctg tacttagcat ctttattgct
     6781 cgcacgatac ttccaggcga aaccaacagc catcaagatt gcgggaataa cgacaatcaa
     6841 catcaggcca aatgccgtca gtatcagtga acgttgctcc agaccaatct gtcctttggg
     6901 atctaacagc gcagaattac agccactgag caatacagtg cctgcaaata atgacaacca
     6961 tcccaaactt ttattgtatt tcctgagtct catttaacga cctcaattcc acgggatctg
     7021 gtggcgttta aagtgtgtgg gcattttacg ggaaggttac attactgtaa acatgattaa
     7081 attcctgtta cttggatttg gctgtcaggt cacatatgtt gcaaaacata tcaagttgat
     7141 tttaagaagc tgttgcaaaa gggcgttata acgaaagggg agtgcagaag gagtttggac
     7201 g
//
LOCUS       DAPPNJ010000209         7185 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000209 DAPPNJ010000000
VERSION     DAPPNJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.211"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(219..812)
                     /gene="tehB"
                     /locus_tag="RPM73_003834"
     CDS             complement(219..812)
                     /gene="tehB"
                     /locus_tag="RPM73_003834"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDX6522137.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(809..1801)
                     /gene="tehA"
                     /locus_tag="RPM73_003835"
     CDS             complement(809..1801)
                     /gene="tehA"
                     /locus_tag="RPM73_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDX6522138.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1925..2905
                     /locus_tag="RPM73_003836"
     CDS             1925..2905
                     /locus_tag="RPM73_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDX6522139.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2900..3436)
                     /gene="rimL"
                     /locus_tag="RPM73_003837"
     CDS             complement(2900..3436)
                     /gene="rimL"
                     /locus_tag="RPM73_003837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDX6522140.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3499..3723)
                     /locus_tag="RPM73_003838"
     CDS             complement(3499..3723)
                     /locus_tag="RPM73_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDX6522141.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3863..5518)
                     /gene="opgD"
                     /locus_tag="RPM73_003839"
                     /gene_synonym="mdoD"
     CDS             complement(3863..5518)
                     /gene="opgD"
                     /locus_tag="RPM73_003839"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDX6522142.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5743..7086)
                     /locus_tag="RPM73_003840"
     CDS             complement(5743..7086)
                     /locus_tag="RPM73_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX6522143.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         1888 a         1814 c         1693 g         1790 t
ORIGIN      
        1 aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa ggggctaagt
       61 caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga ctactgtgat
      121 taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag taaatcatct
      181 cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg tgccagcatc
      241 gtggcgaaac gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag ctcgccgacg
      301 tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc tccctctttg
      361 aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc cgccacaatc
      421 aggttgtaac caccaggttt agtgcaacgt tgcatattgg caatcaaccc ggggatggtt
      481 ttagcctcaa ggaacatcag caccacagtc gaaagaataa aatcgtactg tccatcaaat
      541 gtgaggttat tcagatcaac gacccgggtg tgtaaattat ccagattttc aatggattta
      601 atgcgctcga cgttggcgat actcatggca tttttatccc atgcgtcaac atcataacca
      661 ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag cgttttaccc
      721 ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa ttcatattta
      781 tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc attaaaacgg
      841 cgcgctcggt tctgactaac aattttccct gcatcagaag cgcgaaagta cggatgagca
      901 gtattgcaat aataaaattg gtaaagataa acagcggtac cgccagcgta tggaaaaatc
      961 cattttcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat acgccgaacg
     1021 agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc agacgtagca
     1081 taaacagcag ttgcagcagt ccataaccaa aaagcatttt cgccagcgta tcaccctcgc
     1141 cgccgttgac gctcaaccaa gcactacagg ccaccagcgc aggagcgagc tgaatgccga
     1201 gtgatgtccg cagtgccgtg ggtaattctc ccgaactgcg cagacgctgc aagatcacag
     1261 gttcaaggct tagccatgag aaaacgcctg cgcctaaaaa caccagaccg gcgtcggtgt
     1321 agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc ggcagataca
     1381 gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca gtttgccagg
     1441 cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc agtgggcgaa
     1501 accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa ctcacaaaac
     1561 tgctcatcac tggatggcgc acttctgcca gcacgctatg cggaaagcgt atgagtcggg
     1621 taataaatgc gctagttaac aatccccaga tgatcatcgc cagaatcacc agcccatccc
     1681 ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat cccatcccta
     1741 tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta tcgctctgca
     1801 tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt atggaatttt
     1861 ctcgagcata gccagagccg cagaatttgc tacggttata gtaagtcacc tgtgggaaaa
     1921 cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc aggaagatgg
     1981 cactaaaaaa aatgtgttat tacggcagat tatcgccatt agcgatttta acgatgtgat
     2041 agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg aaggaaattg
     2101 ctggatttac gaccagaacg ctattgcgtt cggcggagcg gtgatttccg gtaatacgcg
     2161 catcaccggc accagcgtgc tgtggggaga ggtttacgca acggataatg tctggatcga
     2221 taatagtgaa atcagccagg gtgcatatat aagtgacagc gtcaccattc atgactcact
     2281 ggtttgcggg caatgtagaa tatttggtca cgccttaatt aatcaacatt ctatgatcgt
     2341 cgcagcacaa ggcttaacgc ctgaccatca actcctgcta caaatttatg atcgcgcaag
     2401 ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg tcatccggta
     2461 tgcttttatt gaacatcgtg ccgaagtttt tgattttgcc agtattgaag gtaatgaaga
     2521 gaacaacgtc tggctatgcg attgcgctaa agtctacggt cacgcgcagg tgaaagctgg
     2581 catagaagaa gatgccattc caacgattca ttacagttcg caggtggcgg aatatgccat
     2641 tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg ccgtggtacg
     2701 tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc gtattacagg
     2761 tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccgtcgttg aagcctttga
     2821 tggcgacacg gttcatgtcc gtggtccaaa agtcattaac ggcgaggagc gcattacgcg
     2881 aacgccgctg gcagggttat tatgaatcga taatacgcgc gtataagttc acatcatcat
     2941 aggcatcatt caggaactca gcctgtttca ggcaaccttc aaggataaaa ccattacgca
     3001 aagcgacctg gttgctttgc ggattgtcca cccgacattt gatcacgaag cgtctaagtt
     3061 caccagactg ggcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg atcccctgcc
     3121 cctgatgaga ttcgtccagc cagtagccta tttcagcggt tttattcagt ggttcaatac
     3181 gattaaacga gataacgccg ataagttcat cttctttgaa aatcatgaac attttggcat
     3241 agccgcgttg atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc tcttcactct
     3301 gaacgaactg cggccagttt agcgactgct gtaaccaggt tttattttta cagattaact
     3361 gataaagagg tgtgacgtga ctttccgcaa cagcatgtaa ttcaagtgat tcgcttactt
     3421 ttatcgtttc agtcatctta tctccagtca atccatgctg ttgttaaaaa agccgcctgg
     3481 ctggcggctt aggaaagttt acacctcttt gacgctctcc tgctgcagga ttttgagcat
     3541 ctcatctttt aactgtagct tttgtttttt catgcggacc acttccgcat tgtaccctcg
     3601 accgtcggaa ccttcctttc tggcaatttc atgatcaagt ttattgtgtt tatcgaacaa
     3661 ggacataaag cgaggatttt cgtttttcag acgggatatt aaatctcggt attctggaaa
     3721 catacgcact ccctgttagt ggctgacaag gtgtatctga aatacacacc atcagattac
     3781 tcatttaact tagggaaaaa tgcgagcggg atcgtgttta accagacggc acctcccgaa
     3841 gaaggtgccg aagaaaaaac gattaactca tcacgcggtc gtcaacatac tgacgtttat
     3901 ccggcgctgg cgggaaatac tgatacagcc atgtttcact gatagcgtcc ccctgacaac
     3961 gtagatacat ccgcatatcg accggatcag tggagtccga agtcggatac cagtcaaact
     4021 gaatacgata accatcgatg ggttcaatat agagaatttc gatttgcttc gcttccccac
     4081 tggaaagcgt aatcaccggc tcaatgcctt ttggcgcggc agctttcaga tcaccaccaa
     4141 cgaaatcgac ggcaaaacga cgcgcccatt tttcgggata gtgttcacct ggcgcccaac
     4201 cttccgggaa accgcccatg ccggtacgcg tcgccataac gcgcgctaat gggcaatgaa
     4261 caggcggttg cgcactccag tacagacgat actggaatgc aaactcatca cctgctttta
     4321 cagctttttc tggctgccag aagcagacaa tgttatccag cgtttcgccc gttgttggga
     4381 tttccatcag gccgatggtg cccttacccc acttgttacg cggttccacc cacagacttg
     4441 ggcgtttgtt ataccagccc ataatgtcct gataatggga gaagtcacga tccagttgca
     4501 ataaaccaaa ccctttcggg ttgttgtcgg tgtaagcatt gaactgcaat ttttgcggat
     4561 tattcagcgg acggcaaatc cactcgccgt tgccccgcca catggacaga cgatcagagt
     4621 catgaatttg cggatgaatg gtgtcgcaca tccgacgttc attagtaccg cagctgaaca
     4681 tactggtcat cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac aggtgatttt
     4741 ccacatccat aatcacctga cttttctcac aatggatagt gaacttatag gcaccagtaa
     4801 tgctggcgct atcgagcaac gcataaacgg taaaggtagt tgcccccggt tttaccgtat
     4861 caaaccagaa ggcggtaaag tcggggaact cttctttact gtcggtgtaa gtgtcaatcg
     4921 ccaggccgcg ggccgacaaa ccgtattgat atgtgtcatc aacggcgcgg aaataactcg
     4981 ctccgaggaa tgatactaca tcacggcgcg ccagttcggg ggctttaaac acgcgaaaac
     5041 cggcaaagcc gagatcgctt tgcccttcta attgtttggt atcaacacct gcatcgttgt
     5101 atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct ggatctacag
     5161 aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga gcgtccagtt
     5221 gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata ctgttataag
     5281 cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca cgccacgctg
     5341 tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc tgggtttgcc
     5401 cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa gcaatgccgc
     5461 tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta cgatccatac
     5521 ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt cagcggcaag
     5581 aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc aattgttgaa
     5641 ctcagagaat atgcataaat cggcaatttt tttatgtcga tgggatgagc ctgattcgtt
     5701 tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca aaccacagcg
     5761 acgtttactg cgttcttccg cttcctgata aagctggaac tcatccaaaa ccggacaacc
     5821 caacgcctgc tcaaatgcct cgcgactggc attaccgtga ttgcgtgcct gcgtttcatt
     5881 acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt cataggtaat
     5941 gggttgcgcc gcgacccaac cacgttcaat taagggctgt ggatcgtctc cgggatgaat
     6001 cgcctgacga tgcgcctcac ctgaaggcgt cagacggtac cggaaccatg ccagcccttg
     6061 ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta aatgcatttg
     6121 gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat acagttgtcg
     6181 ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg cggtatgcgt
     6241 gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc tggtctggcg
     6301 tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg gttcaattcc
     6361 gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg tcaggtggtt
     6421 gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt tgtgcaaggc
     6481 gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt ccagggcttc
     6541 ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat attcctctaa
     6601 cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg ccgctttctg
     6661 gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa cgcggaaggg
     6721 attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta ccggcacccc
     6781 tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa acatccgccg
     6841 aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt caacattcag
     6901 acgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt tttccagcac
     6961 agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa cttcttgctg
     7021 gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg tgatgctgtt
     7081 cgccatagtg tctgcctcca gtggtcagaa atctggagtg taggtaaccg cattcactct
     7141 tgcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaa
//
LOCUS       DAPPNJ010000210         7185 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000210 DAPPNJ010000000
VERSION     DAPPNJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.212"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..233
                     /gene="yafQ"
                     /locus_tag="RPM73_003841"
     CDS             <1..233
                     /gene="yafQ"
                     /locus_tag="RPM73_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDX6522144.1"
                     /translation="KLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYR
                     DAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            389..1129
                     /locus_tag="RPM73_003842"
     CDS             389..1129
                     /locus_tag="RPM73_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDX6522145.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1100..1867)
                     /locus_tag="RPM73_003843"
     CDS             complement(1100..1867)
                     /locus_tag="RPM73_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDX6522146.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2073..2651)
                     /gene="lpcA"
                     /locus_tag="RPM73_003844"
     CDS             complement(2073..2651)
                     /gene="lpcA"
                     /locus_tag="RPM73_003844"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDX6522147.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2891..5335
                     /gene="fadE"
                     /locus_tag="RPM73_003845"
     CDS             2891..5335
                     /gene="fadE"
                     /locus_tag="RPM73_003845"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDX6522148.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5378..5824)
                     /gene="ivy"
                     /locus_tag="RPM73_003846"
     CDS             complement(5378..5824)
                     /gene="ivy"
                     /locus_tag="RPM73_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDX6522149.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6005..6775
                     /gene="yafV"
                     /locus_tag="RPM73_003847"
     CDS             6005..6775
                     /gene="yafV"
                     /locus_tag="RPM73_003847"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDX6522150.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(6817..>7185)
                     /locus_tag="RPM73_003848"
     CDS             complement(6817..>7185)
                     /locus_tag="RPM73_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDX6522151.1"
                     /translation="LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAESGLS"
BASE COUNT         1665 a         1827 c         1923 g         1770 t
ORIGIN      
        1 taaaacttgc acaaaagcgt cataaggata tgaataaatt gaaatatctt atgacgcttc
       61 ttatcaataa tgctttaccg cttccagctg tttataaaga ccacccgctg caaggttcat
      121 ggaaaggtta tcgcgatgct catgtcgaac cggactggat cctgatttac aaacttaccg
      181 ataaactttt acgatttgag agaactggaa ctcacgcggc gctctttggg taaagcacgc
      241 tgccgatgaa atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa
      301 tgtgagcctt ccaaatccag actattccga tttgggatct attttgccgt cctggcgtgt
      361 gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt
      421 gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt
      481 gagcaaagag tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga
      541 agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta
      601 taaaatctgt aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag
      661 cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa
      721 agcgattaat attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata
      781 cctgatgatc cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat
      841 tgatgagatc ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt
      901 gagtatttac ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt
      961 taaggacttc tgggagcagc tgaagccggg ctacgactac tttgagcaaa cccgtaagcc
     1021 accgaccgtt tctgtggtca atggccgtta cgtggtcagc aagccgttaa gccacgaagt
     1081 tgtgcagcca caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc
     1141 ctggcataat cttttgccag gtttcattgc ccgtcagcgg ctgtgttgca atcaccgtga
     1201 ccacatcatt cggtgtggtc tgcgagctga agtcgatttc cacatcctga tccagcagcg
     1261 ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa attagtcgag caatacgcca
     1321 tcacatagcg cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc agttcatccg
     1381 ccagtgaggc gatatattta aacactgccg ccatgttgcc cggtgtgcgc gggtaacgct
     1441 gcgttaattt atgcaggagc cagcagaagg ctttttcgct atcggtttca ccgaccgggc
     1501 ggaagttgcc ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc
     1561 agttacgccc ccataactcg cgggtaaatg ggtgagtatt ttccagcgcc acctcgcccc
     1621 gattagcctg gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa
     1681 gtttggcgat gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac
     1741 cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta
     1801 caagtccggt gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt
     1861 cgcacatggg taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa
     1921 gaattttcag gtcggatgcg gcgtaaacga attattcggc ctacggttcg ggtacagatt
     1981 tgtaggcatg ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc
     2041 ggcgtaaacg ccttatccag cctacgccag acttacttaa ccatctcttt ttcaattaac
     2101 tggatcagga tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag
     2161 tgcggtacgc gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc
     2221 agggtgatca ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca
     2281 gagttaccgg aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg
     2341 tagcgggaga aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg
     2401 tcagaaatag caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct
     2461 tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca
     2521 ccggctttaa agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg
     2581 tcatctttta aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga
     2641 tcctggtaca tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga
     2701 taccgccaaa agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg
     2761 cggataaaaa cagcaacaat gtgagctttg ttgtaattat attgtaaaca cattgctaaa
     2821 tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg
     2881 gcttttcgtt atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt
     2941 ctatcaccgc gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct
     3001 cggcgttgct ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc
     3061 atttaacttt gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg
     3121 taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg
     3181 gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca
     3241 gccgcgcctg accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg
     3301 gatggcgaat gatttccaga tcacccatga gctggcggat ctgccgccgg agttgtgggc
     3361 gtaccttaaa gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga
     3421 gttctcggct tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga gcgggatcct
     3481 ggcgattacc gtcggcgtgc caaactcatt aggcccgggc gaactgttgc aacattacgg
     3541 cactgacgag cagaaagatc actatctgcc gcgtctggcg cgtggtcagg agatcccctg
     3601 ctttgcactg accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat
     3661 tgtctgcatg ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa
     3721 acgctacatt acgctggcac cgattgcgac tgtgcttggg ctggcgttta aactctccga
     3781 cccggaaaag ctgctgggtg gcgcagaaga tttaggtatt acctgtgcgc tgatcccgac
     3841 caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa
     3901 cggaccgacg cgcggtaaag atgtcttcgt accgatcgat tacatcatcg gtgggccgaa
     3961 aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac
     4021 cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg ctggcaacgg gcgcgtatgc
     4081 tcacattcgc cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct
     4141 ggcgcgtatt gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg
     4201 cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca
     4261 ccgcgggcag cagtcgatta ttgatgcgat ggatatcact ggtggcaaag gcattatgct
     4321 cgggcagagc aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga
     4381 aggggccaac attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca
     4441 tccgtacgtg ctggaagaga tggaagcagc gaagaacaat gacgtcaacg cgttcgataa
     4501 actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg
     4561 cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca
     4621 gcacctgaac cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggctgtgct
     4681 gggcggcagc ctgaaacgtc gtgagcgcat ctcggcacgt ctgggggata ttttaagcca
     4741 gctttacctc gcctctgccg ttctgaagcg ttatgacgac gaaggccgta atgaagccga
     4801 cctgccgctg gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga
     4861 tgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt
     4921 cccgaccgga cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat
     4981 tttacaagtg ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag
     5041 cgagcataat ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc
     5101 aattcatcag cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga
     5161 actggcgcac aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt
     5221 gaaagctgaa gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct
     5281 ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg cgtaattgaa
     5341 taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta aagccatccg
     5401 gatggttttc caggctgccg gtcaacgccg cgaacaacac cgttttacca tcaatcgaaa
     5461 gcgcatcgtt cacattcagc caggtgagtt tctcttgcga cgttttctca tcaatagtcg
     5521 agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata cgttgcgagc
     5581 cacagtcatg cggtttgcac gcgctcatca cctgatacgt ctcatctccc aacgttacgg
     5641 tttgtgcggg agtataagta ccgcctttca tcacccaggc aggcagctta tgcccttgta
     5701 ccatctgatt aaatgcagct ttggtggttt cgccctttgc aaggctgcta atggttaaat
     5761 catcctgcgc cattgcactg gtggcgatga ccagagcggc gactgtcgtt attgccttaa
     5821 acatcattcc tcccgagctt atcctgccca tgttattaac cttctaaagc ctggcgataa
     5881 cacgataatt tgcaaatcag atatttccaa tatcagcatt tgttatcaat ttattctgcg
     5941 ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc cttcgcataa aggagttgta
     6001 caccgtgcct ggtttgaaga ttacgctttt gcagcaacca ctggtgtgga tggatggccc
     6061 tgccaacctg cgtcattttg atcgtcaact ggaaggtatt accgggcgcg atgtgatcgt
     6121 tctaccggag atgtttacca gcggctttgc catggaagcg gcagcttcgt cgctagcaca
     6181 agatgacgta gtgaactgga tgacagctaa ggcgcagcag tgcaatgcgc tgattgcagg
     6241 cagtgttgca ttacaaacgg agtctggttc ggttaaccgc tttttgctgg ttgagccggg
     6301 cggcacggta catttttatg ataagcgtca tctgttccgc atggcagatg agcatctaca
     6361 ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta ttttgccgtt
     6421 ggtgtgctac gacttacgtt ttccggtgtg gtcgcgcaat ctcaacgatt atgacctcgc
     6481 catctacgtt gccaactggc ctgcgccgcg ctctctgcac tggcaggcat tgctgacggc
     6541 ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca gcgatggcaa
     6601 cggctgccat tatcgcggtg acagccgggt gattaacccg cagggcgaga ttatcgctac
     6661 tgccgacgcg catcaggcaa cgcgcattga tgcggagctg tcgatggtgg cattgcggga
     6721 atatagagaa aagtttccgg catggcagga tgcggatgag tttaggttgc ggtgagtggg
     6781 ggttgtgttt aaggctgggg gagagtcggg gtaagactac gaaagcccgc tctccgcaag
     6841 gactgacgcc agatagtttc tgtccatagc tgcttttcgc atcttacgtc ttaaccctgc
     6901 cttgaatacc ttatcattcg tcaaaatatt aatagcgatg tgccgtatcc ctgaaaataa
     6961 ttctgcggcg tttcctcttc ttattttgca gtcgtcttca ttcattacca cgtccagacg
     7021 ccagtgcagc ttattctcca cgtgccagtg gtttcggatt gctgtggcga acttttctgc
     7081 ggttaaatca gcagaactga tataatatct gaccgtcatt tctggctctt tcttttgttc
     7141 tgctattatt gaccgaaagg agactgccac gcataatttc ttcag
//
LOCUS       DAPPNJ010000211         7136 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000211 DAPPNJ010000000
VERSION     DAPPNJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.213"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            115..1701
                     /gene="pdeC"
                     /locus_tag="RPM73_003849"
     CDS             115..1701
                     /gene="pdeC"
                     /locus_tag="RPM73_003849"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDX6522152.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1704..2027)
                     /gene="soxS"
                     /locus_tag="RPM73_003850"
     CDS             complement(1704..2027)
                     /gene="soxS"
                     /locus_tag="RPM73_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDX6522153.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2113..2577
                     /gene="soxR"
                     /locus_tag="RPM73_003851"
     CDS             2113..2577
                     /gene="soxR"
                     /locus_tag="RPM73_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDX6522154.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3123..4472
                     /gene="ghxP"
                     /locus_tag="RPM73_003852"
                     /gene_synonym="yjcD"
     CDS             3123..4472
                     /gene="ghxP"
                     /locus_tag="RPM73_003852"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDX6522155.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLRDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4623..6272
                     /locus_tag="RPM73_003853"
     CDS             4623..6272
                     /locus_tag="RPM73_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDX6522156.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6426..>7136)
                     /locus_tag="RPM73_003854"
     CDS             complement(6426..>7136)
                     /locus_tag="RPM73_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDX6522157.1"
                     /translation="EKILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNRAN
                     IKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNK
                     AILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACM
                     FNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTED
                     NINAVAMKITADIMQHAD"
BASE COUNT         1646 a         1634 c         1864 g         1992 t
ORIGIN      
        1 aatttcaaat caggcaaaag tgctatttat accgtaagat ttatctaaag acgtcggtac
       61 ccagggtttt caccttgcaa tggccgggta taaacaggca ggaaattgat agcaatgagt
      121 catcgtgcac gacaccaatt actggcgttg ccgggcatta tctttttagt tctctttccc
      181 atcattcttt cgctatggat tgccttcctt tgggcaaaat cagaagtgaa taatcagctc
      241 cgaacctttg ctcaactggc actggataaa tccgagctgg tcattcgcca ggcagattta
      301 gtgagcgatg cagctgaacg ctatcagggg caagtttgca ctccagccca tcaaaagcga
      361 atgttgaata ttattcgtgg ctatctttat attaatgaat tgatctatgc ccgtgataac
      421 cattttttat gctcatcgct gatagcgcct gtaaacggct atacgattgc accggccgat
      481 tataagcgtg aacctaacgt ttctatctat tattaccgcg atacgccttt tttctctggc
      541 tataaaatga cctatatgca gcggggaaat tatgtggcgg ttatcaaccc tctcttctgg
      601 agtgaagtga tgtctgatga tccgacattg caatggggtg tgtatgatac ggtgacgaaa
      661 acctttttct cgttaagcaa agaggcctcg gcagcaacgt tttctccgct gattcatttg
      721 aaggatttaa ccgtacaaag aaatggctat ttatatgcga cagtttattc gacaaaacgc
      781 ccaattgcag ccattgttgc gacttcatat caacgtctta taacccattt ttataatcat
      841 cttatttttg cgttgcccgc cggtattttg gggagtcttg ttctgctatt actctggcta
      901 cgtattcgac aaaactattt atctcccaaa cgtaaattgc aacgcgccct cgaaaaacat
      961 caactttgtc tttattacca gccaataatc gatatcaaaa cagaaaaatg tatcggcgct
     1021 gaagcgttgt tacgttggct tggtgagcag gggcaaataa tgaatccggc agagtttatt
     1081 ccgctggcag aaaaggaggg gatgatagaa cagataactg attatgttat tgataatgtc
     1141 ttccgcgatc tgggcgatta cctggcaaca catgcagatc gctatgtttc tattaacctg
     1201 tcggcctccg attttcatac gtcacggttg atagcgcgaa tcaatcagaa aacagagcaa
     1261 tacgcggtgc gtccgcagca aattaaattt gaagtgactg agcatgcatt tcttgatgtc
     1321 gaaaaaatga cgccgattat tctggctttc cgccaggctg gttacgaagt ggcaattgat
     1381 gattttggta ttggctactc taacttgcat aaccttaaat cattgaatgt cgatattttg
     1441 aaaatcgaca aatcgtttgt tgaaacgctg accacccaca aaaccagtca tttgattgcg
     1501 gaacacatca tcgagctggc gcacagcctg gggttaaaaa cgatcgctga aggcgtcgaa
     1561 actgaggagc aggttaactg gctgcgcaaa cgcggcgtgc gctattgcca gggatggttc
     1621 tttgcgaagg cgatgccgcc gcaggtgttt atgcaatgga tggagcaatt acccgcgcgg
     1681 gagttaacgc gcgggcaata aaattacagg cggtggcgat aatcgctggg agtgcgatca
     1741 aactgccgac ggaaaacgcg ggagaaggtc tgctgcgaga cataacccag gtccattgcg
     1801 atatcaaaaa tcggacgctc ggtggtgcgc aactcaacgg cggccagtaa caggcgacgt
     1861 tggcgaatgt aatcgccaag cgtctgatgc gtcaccgtgc ggaacattcg ttgcaagtac
     1921 cactttgaat agcctgattt ttttgcgact acatcaatgt taagcggctg gtcaatatgc
     1981 tcgtcaatcc atgcgataag atcctgaata attttctgat gggacataaa tctgcctctt
     2041 ttcagtgttc agttcgttaa ttcatctgtt ggggagtata attcctcaag ttaacttgag
     2101 gtaaagcgat ttatggaaaa gaaattaccc cgcattaaag cgctgctaac ccccggcgaa
     2161 gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt tctatgaaag taaagggttg
     2221 attaccagta tccgtaacag cggcaatcag cggcgatata aacgtgatgt gttgcgatat
     2281 gttgcaatta tcaaaattgc tcagcgtatt ggcattccgc tggcgaccat tggtgaagcg
     2341 tttggcgtgt tgcccgaagg gcatacgtta agtgcgaaag agtggaaaca gctttcgtcc
     2401 caatggcgag aagagttgga tcggcgcatt cataccttag tggcgctgcg tgacgaactg
     2461 gacggatgta ttggttgtgg ctgcctttcg cgcagtgatt gcccgttgcg taacccgggc
     2521 gaccgcttag gagaagaagg taccggcgca cgcttgctgg aagatgaaca aaactaaagc
     2581 gccacaaggg cgctttagtt tgttttccgg tctttgtctt tctctctatc ccgctggtac
     2641 acaggagggt ttcccccgac gtcaacacac ctcattcgag cacgtggtgg aggttccggt
     2701 tggtgttgat gctttaattg tatgtcaccg acgtttcttc gccagtgtaa aagtatactt
     2761 tttaaccgca atatttttgt catctcagac gattttttat cgcaatcctg aacggtatac
     2821 ggctcgataa cgctgcaatc ttgcgcaccg acgataacgt ttgcgcatca attgcctggt
     2881 ttttcatcgt caagacaata aaagagaaaa aagcagcaaa cttcggttga aaaagccgct
     2941 atgatcgccg gataatcgtt tgcttttttt accacccgtt ttgtatgcgc ggagctaaac
     3001 gtttgctttt ttgcgacgca gcaaattgtc gcaaacctgg agcaggaaga taacgtttcg
     3061 ctggcagggg attgtccgcc acgcatcttg acgaaaatta aactctcagg ggatgttttc
     3121 ttatgtctac gccatcagcg cgtaccggcg gttcactcga cgcctggttt aaaatttcac
     3181 aacgtggaag cactgtccgt caggaagtgg ttgccgggtt aacaacgttt ctggcgatgg
     3241 tctactcggt catcgtcgtt ccaggtatgt tgggtaaagc aggcttcccg cctgcggcag
     3301 ttttcgttgc aacctgtctg gttgccggac tcggttctat cgtgatgggt ctgtgggcta
     3361 atctgccgtt ggcgattggt tgcgccatct ccctgacggc atttaccgca ttcagcctgg
     3421 tgctggggca acatattagc gtacctgtcg cgctgggtgc cgtgttcctg atgggtgtgc
     3481 tgtttacggt aatttctgcc acgggtatcc gtagctggat tttgcgcaac ttgccgcacg
     3541 gtgtggcgca cggcacgggg attggtatcg gtctgttcct gctgctcatt gccgctaacg
     3601 gtgtcggtct ggtgattaaa aacccgcttg atggtctgcc cgttgcgctg ggtgatttcg
     3661 cgaccttccc ggtgattatg tcactggtag gtctggcggt gatcatcggc ctggaaaaac
     3721 tgaaagtccc tggtggcatt ctgctgacca ttatcggtat ctcaattgtc ggtttgatct
     3781 tcgatcctaa cgtccatttc tccggcgttt tcgccatgcc ttcattgagg gatgaaaacg
     3841 gcaactcact gattggcagc ctggacatta tgggcgcgct gaatcctgta gtcctgccaa
     3901 gcgttctggc gctggtaatg acggcagtat ttgatgccac cggaactatc cgtgccgtcg
     3961 ccggccaggc gaacctgctg gataaagatg ggcaaattat cgacggtggg aaagcactga
     4021 ccactgactc catgagcagc gttttctctg gcctggtggg tgcagctccg gcagcggtat
     4081 acatcgagtc tgcggcgggt acggcggcgg gcggtaaaac cggtttgacg gctatcaccg
     4141 ttggcgtgct gttcctgctg attctgttcc tctctccgct ctcttacctc gttccggggt
     4201 atgcaacggc tccggcgctg atgtacgttg gcctgctgat gctgagcaac gtggcgaaaa
     4261 tcgactttgc tgattttgtt gatgcgatgg cgggtctggt tacggcggta ttcatcgtgc
     4321 tgacctgtaa catcgtaaca ggcatcatga tcggcttcgc gactctggtg attggtcgtc
     4381 tggtttccgg cgaatggcgc aagttgaaca tcggtacggt cgttatcgcc gtggcgctgg
     4441 tgaccttcta tgcgggtggc tgggctatct aatctttcat cggattctga aaacgggtgg
     4501 caatggctgc ccgtttttat tttctccgca cattgtgtga gcttttgcga tattctgaaa
     4561 aaatgagaat tcaggcataa cgtctttctc agatcataaa aaacatcgca aacagggaac
     4621 gcatggaaat cttcttcacc atactgataa tgaccctcgt ggtctcgctg tccggggtgg
     4681 tcactcgtgt catgcccttt cagatcccgc ttccgcttat gcaaatcgcc atcggtgcgc
     4741 tactggcgtg gccgacgttt ggtttgcatg tggagtttga tcctgaactc tttttagtct
     4801 tgtttatccc gccgttgctg ttcgctgatg gctggaaaac gccgacccgt gaatttctcg
     4861 aacatggtcg agagattttc ggcctcgcgc tggcgctggt ggtggtcacc gtggtcggca
     4921 ttggcttcct tatttactgg gtggtgccgg gcattccgct gatccccgcc tttgcgctgg
     4981 cggcggtact ttctccgacc gatgctgtgg cgctctccgg gattgttggc gaagggcgca
     5041 tcccgaaaaa aatcatgggc attttgcagg gcgaagcgtt gatgaatgac gcttccggcc
     5101 tggtgtcgtt gaagtttgcc gtggcagtgg cgatggggac gatgatcttc accgtcggcg
     5161 gtgcgacggt cgaatttatg aaagtagcca ttggcggtat tcttgccggt tttgtggtga
     5221 gctggctgta tggtcgctcg ctgcgattcc tcagccgctg gggcggtgat gaacccgcga
     5281 cgcagattgt tctgctgttc ttgctgccat tcgcttctta tctgattgcc gaacatattg
     5341 gcgtttccgg catcctcgct gcggttgccg ccgggatgac catcacccgc tccggtgtga
     5401 tgcgccgtgc gccgctggca atgcgcctgc gtgcaaacag cacctgggcg atgctggaat
     5461 ttgtctttaa cggcatggtg ttcctgctgt taggtctgca gctgccgggt attctggaga
     5521 cgtcgctgat ggcagcagaa atcgacccta acgtcgaaat ctggatgctg tttaccgata
     5581 ttattctgat atatgcggcg ctgatgctgg tccgtttcgg ctggctgtgg acgatgaaaa
     5641 agttcagcaa ccgcttcctg aagaagaagc cgatggagtt tggttcgtgg accacacgag
     5701 aaatcctgat cgcgtctttc gccggggtgc gtggggcgat cactctggcc ggtgtgctct
     5761 ctatcccgct gctcttgccg gatggtaacg tcttcccggc gcgctatgag ctggtgttcc
     5821 tggcggctgg cgtcattctc ttctcgctgt ttgtcggcgt ggtgatgttg cctattctgc
     5881 tacaacacat tgaagtcgcg gaccattcgc aacaattgaa agaggaacgt attgcgcgag
     5941 cggcaacggc agaagtggcg attgtggcga tccagaaaat ggaggagcgt ctggcggcgg
     6001 ataccgaaga gaatatcgat aaccagctgc ttacggaggt cagttctcgc gtcattggta
     6061 acctgcgtcg tcgcgccgat ggacgtaacg acgttgaaag ttccgtgcag gaagagaacc
     6121 ttgagcgtcg cttccgtctg gcggcattgc gttctgaacg tgctgaactt taccacctgc
     6181 gcgccacgcg ggagatcagc aacgaaacgc tgcaaaaatt actgcacgat ctcgatttgc
     6241 ttgaagcgtt gctaattgag gaaaatcagt aagccgaaag cgacgtaacc aggcccgagg
     6301 taatctgaaa aaggtgcaat cttttgcacc tttaacaggt tagctcgggc taacattcat
     6361 gattctaaaa caaataagtt atctctcttg taaagtcaat tcattggctg ttcttcgact
     6421 taacctcaat ctgcgtgttg cataatatct gcagtaatct tcatagcgac tgcattaata
     6481 ttatcctcag tttgtgacat gccagtatga gaggtacaat cttcttcaaa gaatttctgt
     6541 agtgttacca gggtatcact atcaatgggg atattttcct ccccacggag aatggaaatg
     6601 cccattccct ggacgctagc ctcatcaaaa ttaacccgat agagacgggt attaaacata
     6661 caggcttttg ttaaaataga acagttgaaa tggcagttgc ttaaattggc attattaaaa
     6721 ttaacattag ccatattaga gccggtacat ataaccattt cgaggaatgt atttgtcagc
     6781 cacgtacaaa agagtttgct atcaattaag tttatattct ggaggattgc tttgttcaga
     6841 ttgacgcgag acagatcagc atgttcaaag ttaattaaat cgagtttaga gaaactaagg
     6901 tcggctttaa ataaattaac atatggcgct attactttat aaagatttac ctcagccatg
     6961 tacgccatat aaaaatttga atagtcaagg aaggtgtttt taatattggc tctattcatt
     7021 gctgcattgt agaattgaac atgcatagcg gagcatgcat ttagagttcc gtcggccatt
     7081 tttatctgag cgaaatttga gtcatcgaga atacaattat caaaaagtat tttttc
//
LOCUS       DAPPNJ010000212         7113 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000212 DAPPNJ010000000
VERSION     DAPPNJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.214"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            312..1349
                     /gene="dusA"
                     /locus_tag="RPM73_003855"
     CDS             312..1349
                     /gene="dusA"
                     /locus_tag="RPM73_003855"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDX6522158.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1483..1725
                     /gene="pspG"
                     /locus_tag="RPM73_003856"
     CDS             1483..1725
                     /gene="pspG"
                     /locus_tag="RPM73_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDX6522159.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1891..2874)
                     /locus_tag="RPM73_003857"
     CDS             complement(1891..2874)
                     /locus_tag="RPM73_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDX6522160.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGCKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2957..4372
                     /gene="dnaB"
                     /locus_tag="RPM73_003858"
     CDS             2957..4372
                     /gene="dnaB"
                     /locus_tag="RPM73_003858"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDX6522161.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4425..5504
                     /gene="alr"
                     /locus_tag="RPM73_003859"
     CDS             4425..5504
                     /gene="alr"
                     /locus_tag="RPM73_003859"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDX6522162.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5757..6950
                     /gene="tyrB"
                     /locus_tag="RPM73_003860"
     CDS             5757..6950
                     /gene="tyrB"
                     /locus_tag="RPM73_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDX6522163.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1667 a         1756 c         1891 g         1799 t
ORIGIN      
        1 tttattgggt tcttttttct ctatcaatgc tattagcagg gagatatatc accagagttt
       61 aatgtgtgat tttttattta tcgtcgaacc tggattgttt atcattggcc ttaacaaagt
      121 taacggctaa taagattatt tccatcactt cgtgagagct tcatgccttg agaggatctc
      181 aattttcttt tgcaatgaga caggcgcttc ctgttgttat ggtatagtac cccgctattg
      241 agcctcctga atagtgatgc tgaataacat aaccccatga tatatcgata aaataatctc
      301 tacatttgaa aatgcacggt aattctgaaa tgcaaaaaat caaccaaacc agcgcaatgc
      361 ctgaaaaaac tgacgttcac tggagtggtc ggtttagcgt tgcaccaatg ctcgactgga
      421 cggacagaca ttgccgctat ttcttgcgtc tgctttcccg caatacgttg ctgtataccg
      481 aaatggtgac cacaggggcg attattcacg gtaaaggtga ttacctggcg tacagtgaag
      541 aagaacatcc ggtagcgttg caactcggcg gtagcgatcc ggcggcgctg gcgcagtgtg
      601 caaagctggc agaagcgcgc ggatatgatg agatcaacct gaatgtcggc tgcccgtctg
      661 accgggtgca gaacggcatg tttggtgcgt gtctgatggg taatgcgcag ctggttgccg
      721 actgcgtgaa agcgatgcgc gatgtggtgt cgattccagt gacggtgaaa acgcgtattg
      781 gcatcgatga ccaggacagc tatgaatttc tctgcgattt catcaacacc gtttccggca
      841 aaggcgagtg tgagatgttt atcatccacg cacgtaaagc ctggctttcg gggttaagcc
      901 cgaaagaaaa ccgtgaaatc ccgccgctcg attatccgcg tgtgtatcaa ctgaagcgtg
      961 actttccgca tctgacaatg tcgattaacg gtggtatcaa gtcgctggaa gaggccaaag
     1021 cacacctgca acatatggat ggcgtgatgg tcgggcgcga ggcgtatcag aatccgggta
     1081 ttctggcggc ggtagaccgg gagatctttg gttcctcgga taccgatgcc gatccggtgg
     1141 cggtagtgcg cgccatgtat ccgtacattg agcgtgaact cagccagggg acgtatctcg
     1201 gccatattac ccggcatatg ttgggcttgt tccagggtat tcctggcgcg cggcagtggc
     1261 ggcgttattt aagtgaaaat gcccataaag cgggtgcaga cattaatgtg ctggaacacg
     1321 cgctcaaact ggtggcggat aagcgttaac ttttcaccaa aaagtagtca aattcaccac
     1381 gccctgcgca ccgtcgcggg gcgttttgct gttaaatcaa tagattattt ttggcatgat
     1441 tcttgtaatg ccagcaagag atttcatatt tgggagagca tcatgctgga actacttttt
     1501 gtgtttggct tttttgtcat gctgatggtc accggcgttt cgttgctggg cattatcgcc
     1561 gcgctggttg tggcgacggc cattatgttc ctcggcggta tgctggcatt gatgattaag
     1621 ttgctgccgt ggttactact ggcgattgcg gtggtgtggg ttattaaggc gattaaagca
     1681 ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt actaagggat tgtgcggatg
     1741 atcacaacct aaggttttat ccttagaaca aaataggaat tgataatcaa atctgtcact
     1801 attgcgcctc taacagattc atcgtgctgt accctacata cagccgaact ataaaaagaa
     1861 agggcttccc aggtggaagc cctatttctt ttatggaatc agcaggctgg aaccttgcgt
     1921 cgcccggctt tccagaatct catgcgcacg ctgcgcatcc ttcagcggat atttctgctg
     1981 ctcggcgaca tcgaccttaa tcacaccgct ggcaatcaaa gagaacagtt cattactggc
     2041 ctcggttaat tcctcccgcg tggtgatata gccttgcagg gaagggcgtg tcacatacaa
     2101 cgagcctttt tgattgagaa tgcctaagtt cacaccggta accgcacctg atgagttgcc
     2161 aaaactgacc attaagccgc ggcgttgcag gcaatccagc gaccgttccc aggtgtctct
     2221 gcccacggaa tcgtacacta cgcgcacttt cttacagccg gtgatctctt tcaatcgctc
     2281 gaccagattc tcttcacgat agttaataac ctgccacgcg ccggctttta gcgcgctctg
     2341 cgctttttgc gcggttccta cggtgccgat aagtttcgcg cccagggctt ttgcccactg
     2401 gcaggcaatt aagccaacgc cgccagccgc tgcgtggaac aggaactgtt catcgggttt
     2461 aatttcatag gttttgcgca gcagataata aaccgttaag cctttcagga aagatgctgc
     2521 agcttgttca aaagaaattg ccgcaggcag aatcgccgct ttatccgcat taatgttatg
     2581 cacagagctg taagcgccta acgccgactg cgcatagact acacgatcgc ctgccttaat
     2641 atgctttaca ccactgccga ctttactcac gatgcctgcc gcttcggtac ctaatccgct
     2701 gggtagtgat ggcggcgggt aaaggccgct gcggatatac gtgtcgataa aattgatgcc
     2761 gatggcttta ttttcgacct ggatttcatt ctctgccgga tcggcaggag tgaactctac
     2821 ggcttgaagt acttccgggc caccgtgctt gtgaaattca attcgtgttg ccatgtgtcc
     2881 tccagaacgt atcgtcaggg tctgcttcat atggtaaagt ttcgacccat tctttatctc
     2941 ggtaactcca ttcactatgg caggaaataa acccttcaac aaacagcagg ctgaaccccg
     3001 cgaacgcgat ccacaagttg ccgggctgaa agtgcctccg cactcgatcg aagcggagca
     3061 gtcggtgttg ggcggtttaa tgctggataa cgaacgctgg gacgatgtag ccgagcgtgt
     3121 ggtggcagac gatttttaca cccgcccaca ccgccatatc tttactgaaa tggcgcgttt
     3181 gcaggaaagc ggtagtccta tcgatctgat tacccttgcg gaatcgctgg aacgccaggg
     3241 gcaactcgat agcgtcggtg gttttgctta tctggcagag ctgtcaaaaa atacgccaag
     3301 tgcggcgaac atcagtgcct atgcggacat cgtgcgtgaa cgtgccgttg tccgtgagat
     3361 gatctcggtt gcgaatgaga ttgctgaagc cggttttgat ccgcaggggc gtaccagcga
     3421 agatctgctg gaccttgctg aatcccgcgt ctttaaaatt gccgaaagtc gtgcaaacaa
     3481 agacgaaggg ccgaagaaca tcgccgatgt gctcgacgca accgtggcgc gtattgagca
     3541 gttgtttcag cagccacacg atggcgttac cggagtaaac accggttatg acgatctcaa
     3601 caaaaaaacc gctggcttgc agccgtcgga tttgatcatc gtcgccgcgc gtccgtcgat
     3661 gggtaaaaca acatttgcga tgaacctcgt cgaaaacgcg gcgatgttgc aggataaacc
     3721 ggtacttatc ttctcgctgg agatgccttc agaacagatt atgatgcgtt ctctggcgtc
     3781 gctgtcgcgc gttgaccaga ctaaaatccg taccgggcag ctcgatgatg aagactgggc
     3841 gcgcatttcc ggcaccatgg gtattttgct cgaaaaacgc aatatctata tcgatgactc
     3901 ctccggcctg acgccaacgg aagtgcgttc ccgcgcacgc cgtattgccc gtgaacacgg
     3961 cggcatcggg cttatcatga tcgactacct gcaactgatg cgcgtaccgg cgctttccga
     4021 taaccgtacg ctggaaattg cagaaatctc ccgctcgctg aaagcactgg cgaaagaact
     4081 gaacgtgccg gtggtggcgc tgtcccagtt gaaccgttct ctggaacaac gtgccgacaa
     4141 acgcccggtc aactccgacc tgcgtgaatc tggctctatc gagcaggatg cggacttgat
     4201 catgtttatc tatcgtgatg aggtgtatca cgaaaacagt gatttaaaag gcatcgcgga
     4261 aattattatc ggtaaacaac gtaacggccc aatcgggacg gtacgcctga cctttaacgg
     4321 tcaatggtcg cgcttcgaca actatgcggg gccgcagtac gacgacgaat aataattatt
     4381 ttatgaatta ggtaattaaa gcaaacactt atcaaggaac acaaatgcaa gcggcaactg
     4441 ttgtgattaa ccgccgcgct ctgcgacaca acctgcaacg tcttcgtgaa ctggcccctg
     4501 ccagtaaaat ggttgcggtg gtgaaagcga acgcttatgg tcacggtctt cttgagaccg
     4561 cgcgaacgct ccccgatgct gacgcctttg gcgtagcccg tctcgaagaa gctctgcgac
     4621 tgcgtgcggg gggaatcacc aaacctgtac tgttactcga aggctttttt gatgccagag
     4681 atctgccgac gatttctgcg caacattttc ataccgccgt gcataacgaa gaacagctgg
     4741 ctgcgctgga agaggctagc ctggacgagc cggttaccgt ctggatgaaa ctcgataccg
     4801 gtatgcaccg tctgggcgta aggccggaac aggctgaggc gttttatcat cgcctgaccc
     4861 agtgcaaaaa cgttcgtcag ccggtgaata tcgtcagcca ttttgcgcgc gcggatgaac
     4921 caaaatgtgg cgcaaccgag aaacaactcg ctatctttaa taccttttgc gaaggcaaac
     4981 ctggtcaacg ttccattgcc gcatcgggtg gcattctgct gtggccacag tcgcattttg
     5041 actgggtgcg cccgggcatc attctttatg gcgtctcgcc gctggaagat cgctccaccg
     5101 gtgccgattt tggctgtcag ccagtgatgt cactaacctc cagcctgatt gccgtgcgtg
     5161 agcataaagc cggagaacct gttggttatg gtggaacctg ggtaagcgaa cgtgataccc
     5221 gtcttggcgt agtcgcgatg ggctatggcg atggttatcc gcgcgccgcg ccgtccggta
     5281 cgccagtgct ggtgaacggt cgcgaagtac cgattgtcgg gcgcgtggcg atggatatga
     5341 tctgcgtaga cttaggtcca caagcgcagg acaaagccgg ggatccggtc attttatggg
     5401 gcgaaggttt gcccgtagaa cgtatcgctg aaatgacgaa agtaagcgct tacgaactta
     5461 ttacgcgcct gacttcaagg gtcgcgatga aatacgtgga ttaatcgttc tgtaatattt
     5521 gattgtctgt gccggatgcg gcgtgaatgc cttatccggc caataaaatc ctaaaaattc
     5581 aataagttga tgttctttca tgctcttata aaggtcgtgc ctctggcgga tgtacgtttg
     5641 tcatgagtct cactctgttg ctaattgccg ttcgctcctg aacatccact cgatcttcgc
     5701 cttcttccgg tttattgtgt tttaaccacc tgcccgtaaa cctggagaac catcgcgtgt
     5761 ttcaaaaagt tgacgcctac gctggcgacc cgattcttac gcttatggag cgttttaaag
     5821 aagaccctcg cagcgacaaa gtgaatttaa gtatcggtct gtactacaac gaagacggaa
     5881 ttattccaca actgcaagcc gtggcggagg cggaagcgcg cctgaatgcg cagcctcatg
     5941 gcgcttcgct ttatttaccg atggaagggc ttaacagcta tcgccatgcc attgcgccgc
     6001 tgctgtttgg tgcggaccat ccagtactgc aacaacagcg cgtagcaacc attcaaaccc
     6061 ttggcggctc aggggcattg aaagtgggcg cggatttcct gaaacgctac ttcccggaat
     6121 caggcgtctg ggtcagcgat cctacctggg aaaaccacgt agcaatattc gccggggctg
     6181 gattcgaagt gagtacttac ccctggtatg acgaagcgac taacggcgtg cgctttaatg
     6241 acctgttggc gacgctgaaa acattacctg cccgcagtat tgtgttgctg catccatgtt
     6301 gccacaaccc aacgggtgcc gatctcacta atgatcagtg ggatgcggtg attgaaattc
     6361 tcaaatcccg cgagcttatt ccattcctcg atattgccta tcaaggattt ggtgccggta
     6421 tggaagagga tgcctacgct attcgcgcca ttgccagcgc tggattaccc gctctggtga
     6481 gcaattcgtt ctcgaaaatt ttctcccttt acggcgagcg cgtcggcgga ctttctgttc
     6541 tgtgtgaaga tgccgaagcc gctggccgcg tactggggca attgaaagca acagttcgcc
     6601 gcaactactc cagcccgccg aattttggtg cgcaggtggt ggctgcagtg ctgaatgacg
     6661 aggcattgaa agccagctgg ctggcggaag tagaagagat gcgtactcgc attctggcaa
     6721 tgcgtcagga actggtgaag gtattaagca cagagatgcc agaacgcaat ttcgattatc
     6781 tgcttaatca gcgcggcatg ttcagttata ccggtttaag tgccgctcag gttgaccgac
     6841 tacgtgaaga atttggtgtc tatctcatcg ccagcggtcg catgtgtgtc gccgggttaa
     6901 atacggcaaa tgtacaacgt gtggcaaagg cgtttgctgc ggtgatgtaa tgcaggaaag
     6961 caggctggag ctacccagcc tgcagtgaaa ttaaactgtc gtcgctttca ctctttcttt
     7021 atagatgatt tttttgatgc catcgttcta cgtgagagat aataaacgtt attagttctt
     7081 ttattgttaa gcttatccta attatctgga att
//
LOCUS       DAPPNJ010000213         7087 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000213 DAPPNJ010000000
VERSION     DAPPNJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.215"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            73..1407
                     /gene="adeQ"
                     /locus_tag="RPM73_003861"
                     /gene_synonym="yicO"
     CDS             73..1407
                     /gene="adeQ"
                     /locus_tag="RPM73_003861"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDX6522164.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1460..1912
                     /locus_tag="RPM73_003862"
     CDS             1460..1912
                     /locus_tag="RPM73_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDX6522165.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2123..3313
                     /gene="nepI"
                     /locus_tag="RPM73_003863"
     CDS             2123..3313
                     /gene="nepI"
                     /locus_tag="RPM73_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDX6522166.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3354..3647)
                     /locus_tag="RPM73_003864"
     CDS             complement(3354..3647)
                     /locus_tag="RPM73_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522167.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3869..4687
                     /gene="nlpA"
                     /locus_tag="RPM73_003865"
     CDS             3869..4687
                     /gene="nlpA"
                     /locus_tag="RPM73_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDX6522168.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSGSDTKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKSVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4691..5614)
                     /locus_tag="RPM73_003866"
     CDS             complement(4691..5614)
                     /locus_tag="RPM73_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX6522169.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5725..6909)
                     /locus_tag="RPM73_003867"
     CDS             complement(5725..6909)
                     /locus_tag="RPM73_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDX6522170.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFCLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1774 a         1595 c         1794 g         1924 t
ORIGIN      
        1 attatcactg aaccgttgtt gtcatttgtt ttacaaaagc aagggattaa atctcctcca
       61 atggacaaaa aaatgaataa tgacaatacc gattatgtga gtaatgaatc agggacgctt
      121 tcgcgattat ttaaactacc tcagcatggg accaccgtcc gcacagaatt gattgcgggg
      181 atgaccactt ttttaaccat ggtgtacatc gtttttgtga acccgcaaat cctcggcgcg
      241 gcacaaatgg acccgaaagt ggtgtttgtt accacctgtt tgattgccgg tatcggcagt
      301 attgcgatgg ggatatttgc taacttaccc gtggcgctgg ctccggcaat ggggctgaac
      361 gccttctttg ccttcgtggt cgtgggggcg atgggcatct cctggcagac cgggatgggc
      421 gcaatattct ggggcgcagt tggactattt ttgctcacgc tgtttcgtat ccggtactgg
      481 atgatctcca acattccctt aagtttacgt attggtatca ccagcggaat tggattattt
      541 atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga cacgctggtg
      601 atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt ttttattata
      661 accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt ggtgacgtct
      721 tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat tccgcctgat
      781 attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga actcgccggt
      841 atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt aattggtgta
      901 actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa taaggcgctg
      961 tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc tgttactgcc
     1021 tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac tgcggttgtg
     1081 gttggcgtta tgttcctgtt ggttatgttc ttctcaccgc tggtggcgat ggttcctcct
     1141 tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag cctggcgcgc
     1201 gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt gatgatgccc
     1261 tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg catcatgaaa
     1321 gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc agctctgttt
     1381 gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata tcatgagttc
     1441 tgattacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg tagtgtttgt
     1501 ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc tcagcgatcg
     1561 gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa agtcagccgg
     1621 tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa acgccgcgca
     1681 ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc tgctgcaatg
     1741 gcatcggatt ttacaaaaag cccgtcttgc cgcgccgatt accgatgctc aggtcaggct
     1801 ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg cttttcagat
     1861 gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt aaaaccgctt
     1921 cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt tcacgccgca
     1981 tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca acaccgtaaa
     2041 agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg cttttcccct
     2101 gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg gcgcggatgc
     2161 catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgcgtcg cctgtctgat
     2221 tatcgttgag tttttgcccg tcagtttgtt gacgcctatg gcccaggatt taggcatttc
     2281 ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt ttgccagttt
     2341 gtttattacc cagacaattc aggccactga ccgccgctac gttgttattt tgtttgccgt
     2401 tttgctgacg ctctcctgtt tgctggtttc ctttgccaac tcattcagtt tgcttttaat
     2461 cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg cgtcgctgac
     2521 catgcgtctg gtaccgccgc gtacggtgcc gaaggcgctg tcggtgatct tcggcgcggt
     2581 ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc ttatcggttg
     2641 gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgttg tgtattttct ggattatcaa
     2701 atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt tccgcttatt
     2761 acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg ccgggcagtt
     2821 tgctttcttc acgtatattc gcccggtgta tatgaacctg gcgggattcg gcgtggatgg
     2881 cttaacgctg gtgctgttga gttttggtat cgccagcttt attggtacgt cgctttcgtc
     2941 gttcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa tactggctgt
     3001 gagtgcgttg gtactgacgt tgtggggaag cgataaaatc gttgctaccg gcgtggcgat
     3061 tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga tcacccgctc
     3121 gctggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc agcttgctaa
     3181 tacctgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     3241 gatgttgtcc ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     3301 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     3361 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     3421 gccacgatgg cttcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     3481 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3541 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3601 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3661 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3721 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3781 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3841 aggtaatcat taataaaagg ataaaaaagt gaaactgaca acacatcatc tacgggcggg
     3901 ggccgcatta ttgctggcag gaattctgct ggcaggttgc gaccagagtg gcagcgatac
     3961 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     4021 aaaggtagca aaagagaaat atggtctcga tgttgaactg gtaggattta gcggttcgtt
     4081 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtctttc agcatcgacc
     4141 ctttctcgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     4201 tgtcttcccc atggcgggtt attccaaaaa aatcaaaagt gtggcgcaaa taaaagaagg
     4261 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgtgcgcttt tactgttgca
     4321 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     4381 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     4441 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     4501 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4561 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4621 tcaatcaccc gaagtcgcta aagcggcaga aaccattttt aacggtggcg cggtgcctgg
     4681 ctggtaattc tcactcatgc cgcgccggac gatttatttt tctggcgcgg cgacgggaat
     4741 ccattgaaat caaaatcacc gatgacagaa tcagcagcgt tcccagccag tccggtaatg
     4801 tgaacgtgat ccccagcaac agcaaagaga gtagcgcgct acttaatggt tctgcacagc
     4861 tcaaaatgct ggcttttgga ccgccaatta attgtgctcc tttcaggtac agactaaatg
     4921 tcagggacgt accaatgacc accaaataaa aaaacgccag aatcaaactg ccgttaacga
     4981 caaagtttgt tccttgtctg gcataaaaag gcaacagaat cagaccgcca atcagcatac
     5041 tccagccgac gactggtaac gtgccatagc gggcaattag cgttgaggga taggtggtat
     5101 agaatgcagc agcaaatgcc gaggcaatgc cccagaacaa cgcggcagga gagatcgata
     5161 atgacgtcgg attaccgtgt gtcaccaata aaaaagtccc gaccagcgat gtcaaaatag
     5221 cgcagaaaac cagaatgccc gggcgcgatt tacgcaccag tgagaaccag gcgacgataa
     5281 tcgtcggtga gaggaattgc agcaccgttg ccgtggctgc gttcgatttt tcgatggtta
     5341 gcaaaaaagt gagctgtaca gttagcgcgc caaccacgga aaaaatcagc aggctaatgg
     5401 catctttatg attgttaata atagaaaaga ttttatcgcc atgaacaaat gacagcgtca
     5461 gtagaatcaa accggcgaat atcaaacgcg tcatagtcaa aaactgcgac gacatctggc
     5521 tttgctccat gatgtattgc gcgcagaccc ctgaacttcc ccacaacacg gcggcaatca
     5581 gaacgttcag catccccttt ctggtggaac ccatttttcc ctctgcatac tgttttgtcg
     5641 tttatgtatg caggcatgat agcaaaaatg gcgaggatgg ctctgtttgc gccacgccga
     5701 tttttcttag cccagactgg tgagctaaat atctttaata aacagcaggc aaatcatcgc
     5761 aatacccaac atccctatcg ccagacagaa caacgcatgg tagctccaga tttcaaccat
     5821 aattccgtca acagagccgg cgataatcca gccgacgcgt gaagtatttg catataacgt
     5881 tgtcgcagag cctatttttt caggcatcaa gtcctgaaaa taaagcatgc cgataccaca
     5941 gagaatacca aggaagatgg cattaagaat ttgcaattcc agctcaaccg ccggagtcgt
     6001 cgccatgagt acgctggcgt aaaaacacat tccactcacg atagcaatga gcattaatag
     6061 tcgcttgcca atacgtttca tgtaatagcc tgcgattaac atcatcggaa tttccagacc
     6121 ggcagcgata ccaatcattt ctccagccag tttatcggtt agatgcagtt catcaataat
     6181 aaatagcggc atatttatca tgtagagatt attcgccgcc cacatcattg aacagaccac
     6241 aaaaagtagc cgcgtatccc gcttcctgtg ggtggagggt aaaatttcta cgggttgggt
     6301 aacgacagga atatttcttt gtattgatgg caaaaacaac cagactatca gtccgcaaac
     6361 aacaaatgcg atggctgcgg tgagatacat cactttaaaa ctaaatccca ttgccagttc
     6421 ataagcgagc ggtggcccga taacccaggc aagcgagatc tgagcacgta aaaatgtact
     6481 gaacatgacc gtctcacggc ctgttctgtc ggcgtgttca cgggcgaggg cgaacatttg
     6541 cgggtttgcg gtggaagcaa aactactcag aagtacgccc gttgagagga gaatgaagta
     6601 gttgcgattc cacgcaaaaa gcgtgcaggc cagcactcca aataagcaac atagcagaat
     6661 cagtaattta cggtcgcctt gtttatcgga gtgccttgcc agaaattgac tgaccagaat
     6721 tcccataata gcgctaccgg tgaagaaaaa acctaccatt ataggacggg ctttcagttc
     6781 atctgcgagg aatatactta gggtaggagt ctgaagagcg cccgcaatac ccgtcagaaa
     6841 ggcgacaagt aaaaatgccg cggcagtgag atcaagtatt tttgatggag tggtagccgt
     6901 tttttgcatt cttctgaatc accgggcgaa aaaatataac tgagttacaa ataagtacca
     6961 gtcgaatgag tattgtatta agaatttttg agtatcgctg atacaaccag aaagcaatgc
     7021 tagcgcaata ttatagggcc agcaattgag attattagta aaagcatgtt aagaattaat
     7081 taccgaa
//
LOCUS       DAPPNJ010000214         7065 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000214 DAPPNJ010000000
VERSION     DAPPNJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.216"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            103..1500
                     /locus_tag="RPM73_003868"
     CDS             103..1500
                     /locus_tag="RPM73_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDX6522171.1"
                     /translation="MKNENGEQNSFTAAHTINGSQQVAVIESKGDKTTIESVTLKDIS
                     KYIKDDIGFSYQGYFRSGWGTGNHGSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQD
                     GKTANAVVTYDGNVGEQYNDAWFGDSANENIMQFSDIYLTTRGFLPFAPEADFWVGKH
                     KLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMN
                     TNSVDVRYRNIPLWDDATLSLMAKYSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQ
                     NLQRDTFNEFTLQVANNSYASSFASFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMY
                     LTDNIIMANALVYSHGEDVYSHESGAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQ
                     QNKTQQGVTLNESAYKTTLWHALKVGESILGSRPEIRFYGTYINILDNELSNFKFNEN
                     SKDEFMAGIQAEVWW"
     gene            1525..2964
                     /locus_tag="RPM73_003869"
     CDS             1525..2964
                     /locus_tag="RPM73_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDX6522172.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYAQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(3021..3239)
                     /gene="marB"
                     /locus_tag="RPM73_003870"
     CDS             complement(3021..3239)
                     /gene="marB"
                     /locus_tag="RPM73_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDX6522173.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3271..3654)
                     /gene="marA"
                     /locus_tag="RPM73_003871"
     CDS             complement(3271..3654)
                     /gene="marA"
                     /locus_tag="RPM73_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDX6522174.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(3674..4108)
                     /gene="marR"
                     /locus_tag="RPM73_003872"
     CDS             complement(3674..4108)
                     /gene="marR"
                     /locus_tag="RPM73_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDX6522175.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            4320..4985
                     /gene="marC"
                     /locus_tag="RPM73_003873"
     CDS             4320..4985
                     /gene="marC"
                     /locus_tag="RPM73_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDX6522176.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(5010..6200)
                     /gene="ydeA"
                     /locus_tag="RPM73_003874"
     CDS             complement(5010..6200)
                     /gene="ydeA"
                     /locus_tag="RPM73_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDX6522177.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIAALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6350..7065)
                     /gene="yneK"
                     /locus_tag="RPM73_003875"
                     /pseudo
     CDS             complement(6350..7065)
                     /gene="yneK"
                     /locus_tag="RPM73_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         1944 a         1533 c         1733 g         1855 t
ORIGIN      
        1 tataaatccc gactttcgac cttacaaaaa agcatcacgg aaaacaaata taaatcagcc
       61 tcgcttgctg aaatatcagc gacatctccc gtcgctgata acatcaaaaa tgaaaacggt
      121 gaacagaact cgtttaccgc agcgcatact ataaatggat cgcagcaagt tgccgttatt
      181 gaaagtaaag gcgacaaaac cactatcgaa agcgtgaccc tgaaagatat cagtaaatat
      241 ataaaggatg atattgggtt cagctatcag gggtacttcc gctcaggctg gggtactggt
      301 aatcacggct caccacaaac ttatgcagcg ggttctctgg gacgttttgg taatgagatg
      361 agcggttggt ttgacctgac cttaaatcag cgtgtttata atcaggacgg taaaacggca
      421 aatgcggtcg ttacctatga tggcaacgtg ggtgagcagt ataacgatgc ctggtttggt
      481 gacagtgcca atgaaaatat catgcagttc agcgatattt acctgacaac gcgaggtttt
      541 ttacccttcg caccagaggc agacttctgg gtcggcaaac ataaactccc gcaatatgag
      601 atccagatgc tggactggaa aaccttaacc acggatgtcg ccgcgggtgt ggggattgaa
      661 aactgggcac ttggtgtagg gctgtttgat atgtccttaa gccgtgatga tgtcgatgtt
      721 tactctcgtg atttttcgcg taccagccag atgaatacca attctgtgga tgttcgttat
      781 cgcaatatcc cgttatggga tgatgcgaca ttatcattaa tggctaaata ttccgcacct
      841 aataaaacgg atcaacaaca agataacgaa aatgacaaca gttattttga aatgaaagat
      901 agctggatgc tgacttctgt tttacggcaa aacctgcaac gcgatacgtt taatgaattt
      961 acgttacagg ttgccaataa ttcctatgcc agcagttttg ccagtttctc agatgccagt
     1021 aatacgatgg cgcatggtcg ctattactat ggtgatcata ccaatgggat cgcctggcgt
     1081 ttaatctctc agggcgagat gtatcttact gacaatatta ttatggctaa cgcgcttgtc
     1141 tattctcacg gcgaagatgt ttatagtcat gaaagtggtg ctcatagtga ttttgacagt
     1201 attcgcactg taataagacc ggcctggatc tggaatacat ggaatcagac ggggcttgaa
     1261 ttaggctggt ttaagcaaca gaacaaaact cagcagggtg taacactaaa tgaatcggct
     1321 tataaaacga cactctggca tgcattgaaa gtgggtgaaa gcattttagg ttcacgacca
     1381 gaaattcgct tctatggaac gtatatcaat attctggata acgaattatc gaattttaag
     1441 tttaatgaga acagcaaaga cgagtttatg gccggcatcc aggcggaagt ctggtggtaa
     1501 tctattttag ttaaggagtt gtgtatgtca ggatttaaaa aaggtttttt atggggcggt
     1561 gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg catcagcatc
     1621 gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga aggcgttatc
     1681 gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta taaaacagat
     1741 attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc ctggacacga
     1801 atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt ttatgatgat
     1861 ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc acattttgag
     1921 atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact gatcgacttt
     1981 tttatccgct tcgcatcaac ggtcttcacg cgctataaag aaaaagtaaa gtactggatg
     2041 acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc atttactaat
     2101 tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat aatgtaccag
     2161 gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa atcgatcaat
     2221 cctgaattta acattggctg tatgatcgcc atgtgtccca tctatcctct gacgtgtgca
     2281 cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactggtt tactgatgtt
     2341 catgctcgtg gatattatgc gcaacatatg ctgaattact ttgccaggaa aggattcaac
     2401 ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga ctttatcggc
     2461 tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca actggattat
     2521 gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg gggatggcaa
     2581 attgatccgg cagggctacg ttattcactc aactggttct gggatcattt ccagttgccg
     2641 ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga cggcacggtg
     2701 aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa aaaagccgta
     2761 gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga cctggtttct
     2821 gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa agacaacgaa
     2881 gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg ggatctcatc
     2941 gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg tcaggctctg
     3001 tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac gccgagcgca
     3061 tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg ttcctgcgat
     3121 gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt ttgttccgca
     3181 acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag tggtttcatg
     3241 attgcctcag tgacgttgtc acgttatcaa ctagctgttg taatgattta atggatgtaa
     3301 aaagcgcgat tcgccctgca tattggtcat ccggtattta tgcggcggaa catcaaagta
     3361 atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt ctgccagata
     3421 gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct tacggctgcg
     3481 gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca ggtgccattt
     3541 agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca ggttgtcctc
     3601 gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg acatcgtcat
     3661 acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt gccacttcgt
     3721 ccgccgtcag gttttttgtt aattcttggt gcaggtcctg gccaactaat tgatggcatt
     3781 gttcacatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc ttatcattcg
     3841 ggttcggcaa cctttcaacc cagcctttac agaccaggcg atccagcata cgggtgagtg
     3901 ctcccaggtc gaccgacaac acttttttca gttcaaccgg agtaatacac gccgcgcagc
     3961 ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac agatactcgt
     4021 taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat ggaataattt
     4081 cattgaacag atcgctggta cttttcacat tagttgccct ggcaagtaat tagttgcagg
     4141 ggataatatt gcccaggcaa gtataagtca aatgaatgaa ttgaccgatg ccacgttttg
     4201 ctaaatcggt tcaagttttg gtcaatgcga tgtaaggcta gctaaagagt gacactttat
     4261 ccttgtttac cccttatact tttcgctgat aacccagata cacaggataa caaccaccaa
     4321 tgttagattt gtttaaagca attggcctgg ggctggtggt gttgctgccg ttagctaacc
     4381 cattaacaac tgtcgcgcta tttcttggtc tggcaggcaa catgaacagt gccgaacgta
     4441 atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg gcgtattacg
     4501 ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga attgcaggcg
     4561 gcttaatcgt cgcgtttatc ggttttcgga tgctttttcc gcaacagaaa gcgattgatt
     4621 caccggaggc gaaaagcaag tcggaagagc tggaagatga accgagtgcc aatattgcct
     4681 ttgtaccact ggcaatgcca agtaccgccg gtccgggaac catagcgatg attattagct
     4741 cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg gttgcgccgc
     4801 cgctgatctt tttcctggtt gcggttattt tgtggggaag cctacgcagc tccggcgcaa
     4861 ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg ggattcctgc
     4921 tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt aaaacgtatc
     4981 attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag tgtcactggc
     5041 cagcggcgaa atataatgat tgaccaaatt aacgcggcaa aagcaggcac cgcgcccaca
     5101 taaccaatca tcgacattga ccagtgcaga ctcacctgat tacctaccaa cgcacccgcc
     5161 ccgattccaa tattaaatat cccggagaat agcgccatcg cgacgtcggt ggcatctggt
     5221 gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc gatcccccag
     5281 aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag cagtgccagg
     5341 cacaccagca acagcgcaat cgccgtactt accaacgcag acgcatactg attacccagt
     5401 ttaccgaaaa ttacgctgcc aataatgccc gcaccaccga gtaataacag taatgccgtg
     5461 gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata gctgtatgcc
     5521 gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa tgccgggcgg
     5581 cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag taagggaagt
     5641 aacttaatca ggcacaaaag ggtgataagc gccgcaatac caatcgcgaa gaaggtcatt
     5701 cgccaaccga aatactggcc cacaatacgc ccgagaggta aacctaagac catcgccagt
     5761 gctgtaccgg tggcaattaa actcaatgcc tgtgctcgct tcccggccgg agccatacgg
     5821 atcgccagag acgccgtaat cgaccagaaa atcgcatgcg caaaagccac accaatgcga
     5881 ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacatggct ggcaataaac
     5941 accacaaaca ggcagatcag taatttgcgc cgttcaacct ggctggtcat taacataaaa
     6001 ggcaatgaca ttagcgccac tacccatgca taaatggtca acataatgcc gacctgagcg
     6061 gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac aaattcggtg
     6121 gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc cactttgcgg
     6181 gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt gatgaaatga
     6241 tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc gttttccgtt
     6301 tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt taatgacgtt
     6361 cagcgaggtc tggcgttttg gcgattgcgg gtataggtgg tctgacaggg gagcgaggcc
     6421 tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcattttta caaacagttt
     6481 gagggtcctg cgcgccatat gtttgataat tttatcatca cttaatgatg ctggcatcaa
     6541 cacgtcattc agatcacttt cattgggatt cataaattgg tgatagtgca ataacatatt
     6601 ggcgaaaaat aaaaaaaagt cctgaaaatg aggagattct tgttctgcta aaagggataa
     6661 aaagcagatg cagacttcgg gtagtacatg tttgttagaa cctaatgaat ccatcaggct
     6721 ttgcaataaa tcctctttta attcgctgtc cgttatgtcg agaatggcgg tgttcaagtt
     6781 aggatcttta ttgacgaacc atctgtgaac ctcagagtta ttccataatc gggggagtat
     6841 gacaaaatta ccgcgagaga taaaattcag gacaagcaaa tcaaactggc gtaagtaatc
     6901 tgcagcggta tggtgatccg gaggcagggg ttcaatatgt ggtgtatgcg ctcttttgat
     6961 gggaacgcca gggttgatca tcggaaattg ttcaataaaa tcattcagaa acagaaagag
     7021 cacacgctcg acgccggtct gaaatacgcg ccccatagtg acagg
//
LOCUS       DAPPNJ010000215         7040 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000215 DAPPNJ010000000
VERSION     DAPPNJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.217"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..295)
                     /locus_tag="RPM73_003876"
     CDS             complement(<1..295)
                     /locus_tag="RPM73_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDX6522178.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALG"
     gene            complement(308..2089)
                     /gene="gcl"
                     /locus_tag="RPM73_003877"
     CDS             complement(308..2089)
                     /gene="gcl"
                     /locus_tag="RPM73_003877"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDX6522179.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2179..2994)
                     /gene="allR"
                     /locus_tag="RPM73_003878"
     CDS             complement(2179..2994)
                     /gene="allR"
                     /locus_tag="RPM73_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDX6522180.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVCQGELVRDTARDISTALGLKAHP"
     gene            complement(3072..3554)
                     /gene="allA"
                     /locus_tag="RPM73_003879"
     CDS             complement(3072..3554)
                     /gene="allA"
                     /locus_tag="RPM73_003879"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDX6522181.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3784..4710
                     /gene="allS"
                     /locus_tag="RPM73_003880"
     CDS             3784..4710
                     /gene="allS"
                     /locus_tag="RPM73_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDX6522182.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4779..5873
                     /gene="mnmH"
                     /locus_tag="RPM73_003881"
     CDS             4779..5873
                     /gene="mnmH"
                     /locus_tag="RPM73_003881"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDX6522183.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            5989..6354
                     /locus_tag="RPM73_003882"
                     /pseudo
     CDS             5989..6354
                     /locus_tag="RPM73_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6875..>7040)
                     /locus_tag="RPM73_003883"
     CDS             complement(6875..>7040)
                     /locus_tag="RPM73_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522184.1"
                     /translation="IVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFITNI
                     GAGLPDACAE"
BASE COUNT         1788 a         1847 c         1704 g         1701 t
ORIGIN      
        1 tacccagcgc acgggcataa cgaatcgctg ctgctacgcc atcccgaaac tcttcttcac
       61 ggccaggaat acaggcaata ccgcgctccc ccgccgccca gtcacccgcc ggtaaattgt
      121 gcagcgtatg ttcgagttta ttactcgcca gcacctgttt taattcttca atgtcgtagt
      181 cataaggaaa cataaattca acgccgcgaa aaccacactg cgcagctttc tcaaaacggg
      241 cgagaaaatc atattctcca aataacatcg ataaattagc agagaaacgt aacattattt
      301 atctccctta ttcatagtgc atgaagcagg tttcagtcgg tgcgtccgct gcgttatcgg
      361 cgatatcttc aaattccatg acgttatcca gttcgctgcc catcgaaata ttggtcacac
      421 gctcgagaat aacttccacg actaccggta cccgatattg cgccattaag gctttcgcct
      481 gttcaaaggc tggcgcaata tcttccggtt tgaagacgcg aatcgcttta caacctaaac
      541 cttccgctac ttttacgtgg tcaacaccgt agccattcac ttcactggag ttgatattct
      601 cgaaagcgag ttgcacgcag tagtccatat caaacgcgcg ctgcgactgg cgaatcagcc
      661 ccagataagc gttattgacc agtacatgga tgtacggaat gttgaactgt gcgccaacgg
      721 ccaattcttc aatcaggaac tggaagtcga agtcaccgga aatcgccacc actttgcgct
      781 ctggatcagc ggcacaaacc cccagcgccg ccggaatggt ccagcccagc gggcctgcct
      841 gcccacagtt gatccagtgg cggtctttaa agacatgcag catttgcgca gcggcaattt
      901 gcgacagacc aatggtggtg acatagcaaa catcgcgacc aaaggctttg ttcatctctt
      961 catacacgcg ctgcggtttc accggcacgt tgtcgaaatg ggttttgcgc agcaaagtgc
     1021 gtttgcgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga cccgcttttt
     1081 gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca gagacaatgc
     1141 cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcgata tgaacgattt
     1201 tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa cggttaccga
     1261 taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc gcggtttgca
     1321 gacccaccat cccggccatc agttcatgat cgtccgggat acagccccag cccatcagcg
     1381 ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca gctgcgtcag
     1441 cattaattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt aacatttcta
     1501 cagctttttc gatctgcata cggctggcag caggtttgta gaccggcagc ggttcgtaca
     1561 tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc accagtaccg
     1621 gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacacga ggcaccagcg
     1681 ccgcttcacg tactgtaacc gccattttgc tgaccggctt ggcgatagct tcgatatcaa
     1741 cagcctggaa atcttcttta tgcagacggg cgcgcggagc ctggccggta atacacagaa
     1801 taggaatgga atcagcagaa gcagaataga gcgcggtgat catgtccgtg cccgcaggac
     1861 cggaagtccc cagacatacg ccgatattcc ctgccgttgc gcgggtataa ccttccgcca
     1921 tgtgcgaagc accttccaca tgacgcgcca gaatgtgacg aataccgccg tgcttacgca
     1981 tcgctgagta gaacggattt attgcagctc ccggaacgcc gaaggcggtg gtgatacctt
     2041 ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt ttattcctac
     2101 ctctattaat tggaaaattt ttccaacttt caaattttgt caaaaaaacc cgctccgcag
     2161 agcgggatgc gacagacatt atggatgtgc tttcagtccc aacgccgtgc tgatatcgcg
     2221 ggcggtgtct ctgaccagct caccctgaca gacaaaacga tcttctgtca gtcttgatga
     2281 aggcccggag atggagatag cggcaacaac actacctaca tcatcgtaaa ttgctgaagc
     2341 taagcaattc agacctacaa catgctcttc tttatctacg gtatagccca gttcacgcgc
     2401 ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg taaactgctg
     2461 caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat aaagcagcgc
     2521 tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac acatcctgac
     2581 catcgattta cactctaact gaccaattaa taccgcttca ttgccgttac ggatcgcgac
     2641 attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg cgacggagag
     2701 gacatcgcgg ttatggatgt acgctgcacc gacgttaaag acacctaatc ctatatgcca
     2761 ccagcctaat tgactgtcct gatagacaaa atccgctgcc tgtaaaacct tcagcaagcg
     2821 aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg acgaacttcc
     2881 cccacttttt tccaaatatt gcagaatcgc aattccccgc tctaacgcct gtgcgccctt
     2941 ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg tcatacctac
     3001 gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct tatgcaagtc
     3061 ggttgcaggc gtcacgcaaa acagagttcc tgttcaggaa tactttcaac atcacagttg
     3121 tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc gaaaagtggg
     3181 tgatgccaga cgttacgatg gtaattcact ccctgttcgc cgttggtgat aaacgcccgc
     3241 agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa cacttcacct
     3301 ttcatcggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg aatggtcagc
     3361 ggcagattcg ccggttgcgc gcggttaatg ctgataagcg tacggtcttg ctcaagaatc
     3421 tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg gaaaaaatcc
     3481 cgttgctgcg tttcgattac gtcgccataa gcactaaagg cttcctgact taacggtaat
     3541 acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaatcagc gccgaccggt
     3601 cagcttggtt tcaataacag gcaatctaaa accttaagag aacaacatca atacaaaagt
     3661 tggaatattt ttccaaaact agatttagtc cacaaattga acagggttat catgtaaaaa
     3721 tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag gggatacctg
     3781 aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac aggaagtttt
     3841 tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg cattaaactt
     3901 ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt aacgttgaca
     3961 gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct ggaaagtatg
     4021 ccaagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat tgtcatcaac
     4081 aacctgctct acaaccccca ggccgtcgcc cagttgctgg cgtggctgaa tgaacgttac
     4141 ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga ttcgctatta
     4201 tacgaaggtt tttcactggc tatcggcgtc acgggaaccg aggcgctggc aaataccttt
     4261 agtcttgatc ccttaggatc ggtgcaatgg cgctttgtca tggcggcgga tcatccgctg
     4321 gcgaacgttg aagagccgct aacagaagcg cagttgcggc gctttccggc ggtcaatatt
     4381 gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc gattgcctgg gcaaaaagag
     4441 attattgttc ctgatatgga aacgaaaatc gccgcccatc ttgcgggcgt tggcattggt
     4501 tttttgccaa aatcgctttg ccagtcaatg atcgataatc aacaactagt tagccgggta
     4561 atcccaacga tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt tggcagcggc
     4621 aaagcggtag aagatattgt gaccttgttt acccagcgca ggccggaaat cagcggattt
     4681 ttagaaattt tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg ttggcgcgta
     4741 aaatagcgcg caagctgatt tgaggttcac ttttaactat gcaagagaga cacacggaac
     4801 aggactatcg tgccctgctg attgctgata cgcccattat tgatgttcgc gcccctatcg
     4861 agtttgagca aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat aacgatgaac
     4921 gcgccgccgt tggcacctgc tataaacagc aaggctcaga cgcagcgctg gcgctggggc
     4981 ataaactggt ggcgggtgaa attcgtcagc agcgcatgga cgcctggcgg gcagcgtgcc
     5041 tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg tcagcgctca catattgtgc
     5101 aaagctggtt gtatgcagcg gggattgatt atccgctggt ggaaggcggt tataaggcac
     5161 tgcgccagac cgcgattcag gcgactattg aactggcaca aaaaccgata gtgctgattg
     5221 gcggttgtac tggcagcggt aaaacgctgt tagtgcagca acagccgaac ggtgttgatc
     5281 tggaagggct ggcgcgccat cgcggttcgg cgtttggtcg cacgttacaa ccacaactta
     5341 gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct gaaaaccgac gcccgtcagg
     5401 atttgcgcct gtgggtgctg gaagacgaaa gccggatgat cggttcgaat cacctgccgg
     5461 aatgcctgcg cgagcgaatg actcaggcgg cgattgcggt ggtagaagat ccgtttgaga
     5521 tccgtcttga gcgcctgaac gaagagtatt tcttgcgtat gcatcatgat tttacccacg
     5581 cttatggcga cgaacagggc tggcaggaat attgcgaata cctgcatcac ggactttcgg
     5641 cgattaagcg tcggctgggg ttacagcgct ataacgaact cgctgcccag ctggatacgg
     5701 cgctgacaac gcaactcacc accggcagca ccgacggtca tctggcctgg ctggtgccgt
     5761 tacttaaaga atattacgac ccaatgtatc gctatcagct tgagaaaaaa gcggaaaaag
     5821 ttgtctttcg cggtgagtgg gcggaagtgg cggaatgggt taaggcgcgg taatgaggga
     5881 cgtatttgta ttagtcgcga cttgatctga cacatggcct tgaaaggttg agagttaccg
     5941 gttttgatat gagtgtcgaa tccttatcca aaacaagagg taactctcat gcttcatact
     6001 gccaatcccg tcatcaaaca caaagccggt ttgctcaatc tggctgaaga actcagcaac
     6061 gtatcaaaag cctgtaaaat catgggcgtt tcgcgtgata cgttttatcg ttaccgcgaa
     6121 ctggtcgctg aaggcggtgt ggatgcgcag attaatcgta gtcgccgcgc tcctaacctt
     6181 aagaaccgta ccgatgaggc aactgaacag gctgttgttg attacgccgt cgctttcccg
     6241 acacatggtc agcaccgggc cagcaacgag ctgcgtgaac agggggtttt tatctccgac
     6301 agtggtgtcc gttctgtctg gttactccat aaccttgaga atctcaaaag acgatactga
     6361 acaaatttta tcaggtgacg ttccgtaaaa agttgtatgg agttctcgat gcagtcggcg
     6421 cgactatcag tacccccgct gaaccctgga ctatcgggcc gccgtactgg ggcatgtcac
     6481 cctggcgtgc cgctggtttt ccgctaatgg tttatttcct ctttctttcc cggcaagaat
     6541 ttattttaat ccggaaggat tatttatttt cagataggga ctcacaataa acacgtaaca
     6601 ttttaattaa tgcatggctg aatatgtatt acacaatgtc agatatcgta aaagaccagt
     6661 gcaattctat gtaaactctc tgactgttca ttttatttgt tgtttcaggg tcggaactca
     6721 ccctcaataa acaactgaat acgagtagat attcatcgtc tgagctatat ggctttacac
     6781 aatagccgac atgtcttttc tcagtcagga attttacttt tcctcactac gaagttgacg
     6841 acttcgatat gggatagact cttaattcaa gcaattactc tgcacaagca tcaggcaatc
     6901 cagctccaat atttgttata aaataatgcg taacattaat ttcaccaaca aaaaaaaggt
     6961 ttacaccatc cccttgccct ggtatcttac catatttaca ccacactttt tttgtatctg
     7021 gatcattctt tgatacgatt
//
LOCUS       DAPPNJ010000216         7036 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000216 DAPPNJ010000000
VERSION     DAPPNJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.218"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            553..1542
                     /gene="araF"
                     /locus_tag="RPM73_003884"
     CDS             553..1542
                     /gene="araF"
                     /locus_tag="RPM73_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDX6522185.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1612..3126
                     /gene="araG"
                     /locus_tag="RPM73_003885"
     CDS             1612..3126
                     /gene="araG"
                     /locus_tag="RPM73_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDX6522186.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3141..4127
                     /gene="araH"
                     /locus_tag="RPM73_003886"
     CDS             3141..4127
                     /gene="araH"
                     /locus_tag="RPM73_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDX6522187.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4294..5094
                     /gene="otsB"
                     /locus_tag="RPM73_003887"
     CDS             4294..5094
                     /gene="otsB"
                     /locus_tag="RPM73_003887"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDX6522188.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5069..6493
                     /gene="otsA"
                     /locus_tag="RPM73_003888"
     CDS             5069..6493
                     /gene="otsA"
                     /locus_tag="RPM73_003888"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDX6522189.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6500..6928)
                     /gene="uspC"
                     /locus_tag="RPM73_003889"
     CDS             complement(6500..6928)
                     /gene="uspC"
                     /locus_tag="RPM73_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDX6522190.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1775 a         1633 c         1828 g         1800 t
ORIGIN      
        1 ttttgtatct tgttgatatt aaacgatgaa tttgaattca tgatgaagtg atgattttat
       61 aaaacgtttt ttcatttttg cgagatggct ctcattatac gtgttctgtt aattaatgag
      121 cacggtgata attaatggcg aaatgaatga cgcgcatttt ccacatcttt gagttgtggt
      181 tattacacca tttcaaaaac tcaacgccaa gtaatgcggc ctattgactg gttaaaaaga
      241 agacatcccg catgggtacc aaagacaaca aggatttcca ggctaatctt atggattaat
      301 ctgctgtgca ttcgacaatt tgtctgacaa attggctttc ccttatgtct tttcccgcta
      361 aatttatgca cgttctcact gtaattctgc gatgtgatat tgctctccta tggagaatta
      421 atttctcgct aaaactatgt caacacagtc acttatcttt tagttaaaag gtaatgcttt
      481 gttttccgat taatttaacg aatgtcattc gtttttgccc tacacaaaac gacactaaag
      541 ctggagagaa ccatgcacaa atttactaaa gccctggcag ccattggtct ggcagccgtt
      601 atgtcacaat ccgctatggc ggagaacctg aagctcggtt tcctggtgaa gcaaccggaa
      661 gagccgtggt tccagaccga atggaagttt gccgataaag ccgggaagga tttagggttt
      721 gaggttatta agattgccgt gccggatggc gaaaaaacat tgaacgcgat cgacagcctg
      781 gctgccagtg gcgcaaaagg tttcgttatt tgtactccgg accccaaact cggctctgcc
      841 atcgtcgcga aagcgcgtgg ctacgatatg aaagtcattg ccgtggatga ccagtttgtt
      901 aacgccaaag gtaagccaat ggataccgtt ccgctggtga tgatggcggc gactaaaatt
      961 ggcgaacgtc agggtcagga actgtataaa gagatgcaga aacgtggctg ggatgtcaaa
     1021 gaaagcgcgg tgatggcgat taccgccaac gaactggata cggcacgtcg ccgtacaacg
     1081 ggttctatgg atgcgctgaa agcggccgga ttcccggaaa aacaaattta tcaggtacct
     1141 accaaatcta acgacatccc gggggcattt gacgctgcca actcaatgct ggttcaacat
     1201 ccggaagtta aacattggct gatcgtcggt atgaacgaca gcaccgtgct gggcggcgta
     1261 cgcgcgacgg aaggtcaggg ctttaaagct gccgatatca tcggcattgg cattaacggt
     1321 gtggatgcgg tgagcgaact gtctaaagca caggcaaccg gcttctacgg ttccctgctg
     1381 ccaagcccgg acgtacatgg ctataaatcc agcgaaatgc tttacaactg ggtagcaaaa
     1441 gacgttgaac cgccaaaatt taccgaagtt accgacgtgg tgctgatcac gcgtgacaac
     1501 tttaaagaag aactggagaa aaaaggttta ggcggtaagt aatttgccgg aaaaattccc
     1561 ctctgcatga tgcagagggg gtgtgaacga ccagtgattc acggagacgt tatgcaacag
     1621 tctaccccgt atctctcatt tcgcggcatc ggtaaaacgt ttcccggcgt taaggcgctg
     1681 acggatatta gttttgactg ctatgccggt caggttcatg cgttgatggg tgaaaatggc
     1741 gcaggaaaat caactctctt aaaaatcctc agcggcaact atgcgccaac cacgggttct
     1801 gtagtgatta atgggcagga aatgtccttt tccgacacga ccgcagcact taacgcgggt
     1861 gtggcgatta tttaccagga actgcatctc gtgccggaaa tgaccgtcgc ggaaaacatc
     1921 tatctcggcc agctgccgca taaaggcggc attgtgaatc gctcattgct gaattatgag
     1981 gcgggtttac aacttaaaca tcttggtatg gatattgacc cggacacgcc gctgaaatat
     2041 ctctccattg gtcagtggca gatggttgaa atcgccaagg cgctggcgcg taacgctaaa
     2101 attatcgcct ttgatgagcc aaccagctcc ctttctgccc gcgaaatcga caatcttttc
     2161 cgcgttattc gtgaactgcg aaaagagggg cgggtgatct tatacgtttc tcaccgtatg
     2221 gaagaaatat ttgccctcag cgatgccatc actgtcttta aagatggacg ttatgtcaaa
     2281 acctttaccg atatgcagca ggttgaccac gacgcgctgg tgcaggcgat ggtcgggcgc
     2341 gacattggcg atatctacgg ctggcaacct cgtagttatg gcgaggagcg cctgcgtctt
     2401 gatgctgtga aagcaccagg cgtgcgtacg ccaataagtc tggcggttcg cagtggtgaa
     2461 attgttgggc tgtttggtct ggtaggggcg gggcgtagcg aattaatgaa aggcttgttt
     2521 ggcgggacgc aaatcaccgc cggtcaggtt tatatcgacc aacagccgat cgatattcgt
     2581 aaaccgagcc acgccattgc cgcaggcatg atgctctgcc cggaagatcg caaagcggaa
     2641 ggcattattc ccgtgcactc cgttcgcgac aatatcaaca tcagtgccag acgtaaacat
     2701 gtgctcggcg gttgtgtaat caacaacggt tgggaagaaa acaatgccga tcaccacatt
     2761 cgttcgctca acatcaaaac gccgggcgct gagcaactga tcatgaatct ctcaggcgga
     2821 aatcagcaaa aagccattct gggccgctgg ttatcggaag agatgaaggt cattttgctg
     2881 gatgaaccta cgcgcggcat tgatgttggt gctaagcatg aaatttacaa cgtgatttat
     2941 gcgctggcgg cgcagggtgt ggcggtgctg tttgcctcca gcgacttacc tgaagtcctc
     3001 ggcgttgccg accggattgt ggtgatgcgg gaaggtgaaa tcgccggtga attgttacac
     3061 gagcaggcag atgagcgtca ggcactgagc cttgcgatgc ctaaagtcag ccaggcagtt
     3121 gcctgagtaa ggagagtatg atgtcttctg tttctacatc ggggtctggc gcacctaagt
     3181 cgtcattcag cttcgggcgt atctgggatc agtacggcat gctggtggtg tttgcggtgc
     3241 tctttatcgc ctgtgccatt tttgtcccaa attttgccac cttcattaat atgaaagggt
     3301 tgggcctggc aatttccatg tcggggatgg tggcgtgtgg catgttgttc tgccttgctt
     3361 ccggtgactt tgacctttct gtcgcctccg taattgcctg tgcgggtgtc accacggcgg
     3421 tggttatcaa cctgactgaa agcctgtgga ttggcgtggc agcggggttg ctgcttggca
     3481 ttctctgtgg cctggtcaat ggctttgtta tcgccaaact gaaaataaat gctctgatca
     3541 caacactggc aacgatgcag attgttcgag gtctggcgta catcatttca gacggtaaag
     3601 cggtcggtat cgaagatgaa agcttctttg cccttggtta cgctaactgg ttcggtctgc
     3661 ctgcgccaat ctggctcacc gtcgcgtgtc tgattatctt tggtttgttg ctgaataaaa
     3721 ccacctttgg tcgtaacacc ctggcgattg gcgggaacga agaggctgcg cgtctggcgg
     3781 gtgtaccggt tgttcgcacc aaaattatta tctttgttct ctctggcctg gtatctgcga
     3841 tagccggaat tattctggct tcacgtatga ctagtgggca gccaatgacg tcgattggtt
     3901 atgagcttat tgttatctcc gcctgcgttt taggtggcgt ttctctgaaa ggtggcatcg
     3961 gaaaaatctc atatgtggtg gcgggtatct taattttagg caccgtggaa aacgccatga
     4021 acctgcttaa tatttctcct ttcgcgcagt acgtggttcg cggcttaatc ctgctggcag
     4081 cggtgatctt cgaccgttac aagcaaaaag cgaaacgcac tgtctgatgc ttttttccac
     4141 aacaatttaa cgtttttgcc caccacagcc agccgccaca acggttggct gttcttcatt
     4201 gcaaatggcg acccccgtca cactgtctat acttacatgt ctgtaaagcg cgttctgcgc
     4261 aaaacaataa gaaaagagaa ggaggagagc cgggtgacag aaccgttaac cgaaacccct
     4321 gaactatccg cgaaatatgc ctggtttttt gatcttgatg gaacgctggc ggaaatcaaa
     4381 ccgcatcccg atcaggtcgt cgtgcctgac aatattctgc aaggactaca gctactggca
     4441 accgcaagtg atggtgcatt ggcattgata tcagggcgct caatggtgga gcttgacgca
     4501 ctggcaaaac cttatcgctt cccgttagcg ggcgtgcatg gggcggagcg ccgtgacatc
     4561 aatggtaaaa cacatatcgt tcatctgccg gatgcgattg cgcgtgatat tagcgtgcaa
     4621 ctgcatacag tcatcgctca gtatcccggc gcggagctgg aggcgaaagg gatggctttt
     4681 gcgctgcatt atcgtcaggc tccgcagcat gaagacgcat taatgacatt agcgcaacgt
     4741 attactcaga tctggccaca aatggcgtta cagcagggaa agtgtgttgt cgagatcaaa
     4801 ccgagaggta ccagtaaagg tgaggcaatt gcagctttta tgcaggaagc tccctttatc
     4861 gggcgaacgc ccgtatttct gggcgatgat ttaaccgatg aatctggctt cgcagtcgtt
     4921 aaccgactgg gcggaatgtc agtaaaaatt ggcacaggtg caactcaggc atcatggcga
     4981 ctggcgggtg tgccggatgt ctggagctgg cttgaaatga taaccaccgc attacaacaa
     5041 aaaagagaaa ataacaggag tgatgactat gagtcgttta gtcgtagtat ctaaccggat
     5101 tgcaccacca gacgagcacg ccgccagtgc cggtggcctt gccgttggca tactgggggc
     5161 actgaaagcc gcaggcggac tgtggtttgg ctggagtggt gaaacaggga atgaggatca
     5221 gccgctaaaa aaggtgaaaa aaggtaacat tacgtgggcc tcttttaacc tcagcgaaca
     5281 ggaccttgac gaatactaca accaattctc caatgccgtt ctctggcccg cttttcatta
     5341 tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac ggctatctac gcgtaaatgc
     5401 gttgctggca gataaattac tgccgctgtt gcaagacgat gacattatct ggatccacga
     5461 ttatcacctg ttgccatttg cgcatgaatt acgcaaacgg ggagtgaata atcgcattgg
     5521 tttctttctg catattcctt tcccgacacc ggaaatcttc aacgcgctgc cgacatatga
     5581 caccttgctt gaacagcttt gtgattatga tttgctgggt ttccagacag aaaacgatcg
     5641 tctggcgttc ctggattgtc tttctaacct gacccgcgtc acgacacgta gcgcaaaaag
     5701 ccatacagcc tggggcaaag catttcgaac agaagtctac ccgatcggca ttgaaccgaa
     5761 agaaatagcc aaacaggctg ccgggccact gccgccaaaa ctggcgcaac ttaaagcgga
     5821 actgaaaaac gtacaaaata tcttttctgt cgaacggctg gattattcca aaggtttgcc
     5881 agagcgtttt ctcgcctatg aagcgttgct ggaaaaatat ccgcagcatc atggtaaaat
     5941 tcgttatacc cagattgcac caacgtcgcg tggtgatgtg caagcctatc aggatattcg
     6001 tcatcagctc gaaaatgaag ctggacgaat taatggtaaa tacgggcaat taggctggac
     6061 gccgctttat tatttgaatc agcattttga ccgtaaatta ctgatgaaaa tattccgcta
     6121 ctctgacgtg ggcttagtga cgccactgcg tgacgggatg aacctggtag caaaagagta
     6181 tgttgctgct caggacccag ccaacccggg cgttcttgtt ctttcgcaat ttgcgggagc
     6241 ggcaaacgag ttaacgtcgg cgttaattgt taacccctac gatcgtgacg aagttgcagc
     6301 tgcgctggat cgtgcattga ctatgtcgct ggcggaacgt atttcccgtc atgcagaaat
     6361 gctggacgtt atcgtgaaaa acgatattaa ccactggcag gagtgcttca ttagcgacct
     6421 aaagcagata gttccgcgaa gcgcggaaag ccagcagcgc gataaagttg ctacctttcc
     6481 aaagcttgcg taggagcggt taatcacccg taagtggaac taacagcaca tcgacctcac
     6541 ttgaggcaat cactcttttc gccgagcagg atgctcgcga aaagaaactg tgattgtgat
     6601 taccgcaaat caccaaatcg aaatgatgtt tgtgacatac ttccagaata tgttcgctta
     6661 actcaccgta ggcaataaac gtcttgtcaa cgggataccc cgcatcctga attaacttat
     6721 caagaaagct ttgcgtttct tcttgcatca cgctgcgtaa atcttcaagc atcggcgcgg
     6781 ctaattgatt gtacatttcc gggtcggaag cgagagtaat taaactgatg tgtcccttta
     6841 ctggcctggc gatagatacc gcttttgcca gcagttgctg actttccggt gtaaccgcaa
     6901 cagcgacaag aatattgcta tagctcataa cctgttcctt attctgtgaa cttcaggtga
     6961 cattaaagct ttactcctaa tggagaaacg acgcaatccc aactcggtca aacaggtaaa
     7021 tattagtctg attatt
//
LOCUS       DAPPNJ010000217         6742 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000217 DAPPNJ010000000
VERSION     DAPPNJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.219"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..4848)
                     /locus_tag="RPM73_003890"
     CDS             complement(<1..4848)
                     /locus_tag="RPM73_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HDX6522191.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRRSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWL
                     YRYDERGLFSNITDPAGQTWTQLCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEEHDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELAEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPLWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEAAPGLLYAGQWQDVESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPCGYVDPLGLVGCSTKLGKNMMEAMGLARSTTWK
                     GYRAHHIIPKELWNHPVLQKIKYDIDKATNGIFLRKVDDGVSAMARHQGNHDGYTQVI
                     KDALDKIDINQSTDVITKQIEEIQKIARNGLENGYPVRPLDMDSIGGAAGNSKVYSIW
                     TKIFDK"
     gene            complement(4868..5329)
                     /locus_tag="RPM73_003891"
     CDS             complement(4868..5329)
                     /locus_tag="RPM73_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDX6522192.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5357..>6742)
                     /gene="tssI"
                     /locus_tag="RPM73_003892"
     CDS             complement(5357..>6742)
                     /gene="tssI"
                     /locus_tag="RPM73_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX6522193.1"
                     /translation="AYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRY
                     SAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFAR
                     WQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVP
                     GRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRV
                     KFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGR
                     TYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVL
                     NDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIA
                     GSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDG
                     ITIVGGSVFIN"
BASE COUNT         1218 a         2074 c         1813 g         1637 t
ORIGIN      
        1 tttatcgaaa atcttggtcc aaatagagta aactttagaa ttacctgctg cgccccctat
       61 tgaatccatg tcaagcggtc taactggata accgttttcc agtccattac gagcaatctt
      121 ttgaatttcc tcaatctgtt tagtaataac atcagtggat tgatttatat caattttatc
      181 caacgcatcc ttaattacct gagtatatcc atcatgattt ccctgatgcc ttgccattgc
      241 acttacacca tcgtcaactt ttcttaaaaa gattccattt gttgctttat caatatcata
      301 ttttattttt tgtaagaccg gatgattcca taattctttc ggtataatat gatgtgctct
      361 atatcctttc catgttgtag aacgagctaa tcccatagct tccatcatat ttttcccaag
      421 cttggttgaa caaccaacta gccccagcgg atcaacatac ccacacggat tcggcacata
      481 ccggtaagtc tgctcacccc cctgcaaccc cagcgggtca ctcaccaggt acatcccggt
      541 ttccggctcg taataccgga accggttata gcacagcccg gattccacat cctgccactg
      601 ccccgcatac agcaggccag gggcggcttc cgggtccagt tccccgcgcg ggtccgggta
      661 gccggtgtct gcgggcaggc tgagtgccag cccccacagg ctggtctgcc ccggtcgcca
      721 gacggtttta ccttcactgt taaagagcat cagcggacgg cccgtcaggt cgctcaccgc
      781 atgattcgtc cgcgtcaccc acagcggggc gacggacgga tgcggctgcc gtacccggct
      841 gaactgctgg cttatcagct caaagccgtt aaacaccagg tgccgtacgc tgtacagttt
      901 atcatcgcgg tattcccgga tttccgcaat actgtcgccg tcccacagat acgtgaagcg
      961 gattttcttc cggtcgcagc gtttctctgt tcgtctgccg ctggcgtcgt ggcggtattc
     1021 ccactgctca ccctgtgcgc tgcaataccc ggtcagctgg tcacggctgt cccaccggaa
     1081 ccgctctgtt tccgggcgat agccgtcacg gtgttttgtc cggctgacca tccggcctgc
     1141 ggcgtcatag tcatactgcg tgtttccggc ctgtttcagc cggtggcctc cggcatactg
     1201 gtcagtctct tcacttatcc ggtgcctgcc cgcagactgc gccttcaggt agccacaact
     1261 gtcatagcca taggactcat cgccttcgcc atagcccagc ccggtgcctg aggctgtcac
     1321 cgccgtgacc tggccgttcc ggtccagccg gtattcccgc cgtgtttccc gtccgtaatc
     1381 ttcccggctg gtgacgcctg acacgttacc cgcgctgtcg taacggtact cccgtaccag
     1441 tgagggaacc agcccgcccc atgtggcttc ttccggaaaa aatccggcgc gctggctgac
     1501 aggctgaccg gctgcattcc agccctgttc acaggcaaaa ccccggttac tgtgccggag
     1561 cacttcacgt cccgcctcat cacggctgaa cgtcagcgtc tcatcccccg gcaggataac
     1621 ctctgccagt tctccggcgg cgttgtaacg gtaggtggtt ttcagcaggg attcatcggg
     1681 atgtctgtcg cgttcatctt ccggtggcag gatacagcgg atgacggtgt gttcatcggg
     1741 gtagtggtat tcaatggccc gtcggccctg agtggtgaca ttcacccggc cgcgttcgtc
     1801 gtagccggtg cgctgtgatg tgccgtccgg gctgaagata cccgtcagcc tgccggattt
     1861 gtcataggca tactgcgttt tcccttccgg cgagatgact tctgccaggc ggcctgcggc
     1921 atcccggctg tagaggatgg tttcctccag gccgttaacc ctggcggtac agaggccatc
     1981 ggcgtcatag cggtaacgcc agaccacgcc gccccagtca tgctcttccg tcacccggcc
     2041 gcagttatcc agaacataaa gatatgattc accctgcggg ttaatgactt ctgtcagttg
     2101 cagcgtgtct ttatcgtagc ggtactgcca gcggtggcct tcaccgtccg ttcgcgccac
     2161 cggcaaatcg aacgggccgt actccattgt ccagacaaca ccattgccgt ccgtgtacgc
     2221 cagcaggttg ccgtggcggt catagcgaaa tccttccgcg ccggttccag cacgttcaat
     2281 gccgctcagt ttatggtgct gctgccactg taaccgcact gactgaccgc cggggccgcg
     2341 catcttcacc ggcaggtcgt gaccgctgta ttcggtgtgg tgttcacggc ccatcacgtc
     2401 ggtgagtgtt tccggccggt tgtggtggtc gtactctatg cccagacgcc gttcatcctg
     2461 gcggaatatt cccgacaaca gcccctgagc ggtgtacgcc agccgggtct cttcgccctg
     2521 tggcgacacc aggcttaccg gcaggccgcg ttcatcgcac agctgtgtcc acgtctgccc
     2581 ggcagggtcg gtgatgttgc tgaacaggcc gcgctcatca tagcggtaca accactccgc
     2641 gccgtctgcc gccgtgtaac ggcttaactg accgtgtgcg gcgttgtatt caaacttctc
     2701 cacccgcccg gcaggggagt ggcgggccag caggtgatgg aattcatccc attcatacgt
     2761 ggtggtgctg ccgtccggcg cttcttcccg cagaatattg ccgtcaggat catagtaata
     2821 acggtgagtg cccttgccgt ccgtcacggt ggtgcagccg tcaccatagt ccagcgtggc
     2881 gttgtaatag ccctccgccc cggtgacatt cacgcaccgg ccctgtgcat cgtaggtgaa
     2941 acggctccac gtctggtcgt tatcggacct gcggatgatc cggtccgcag cgtcgtactc
     3001 ataaaacagg tggtagtcca gccgcgcatc cgcttctgtc agccgccccc gcgcatcctg
     3061 ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc
     3121 gctggcatgg tacgtcagtt taagccggat cccgtcggtg tgactgacac ccgtgatccg
     3181 gtactggctg tcgcgctcaa acgtgatgcg gttgctggcg gtgtcactca tcccgcccag
     3241 catatacatc cggtcaccgg ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac
     3301 cgggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggctgc tcagggcatc
     3361 acggtcgaac agctccaggt aatcatcacg gcggcgcagg atgtactggg cgtgatacgg
     3421 gttaaccgcc gtatcacttt caccgtcgaa ggcgaagttc agcgtggctc ccaggctgat
     3481 gatatccacc cggtttccct gttcacgcac ccaggcgtat tcgctccagg agtcgctcca
     3541 gcccacgccg gtcaggccat gcagcggcag tacagaactg tggaaacggg tgaaggagag
     3601 ggggagtgtc tggcccaggg tgaagtcggt acgttcgtcg cagtacgcgc cggttaccgg
     3661 atcgacagga tctgttaccc atctgatgac tttattggca atacttttta acgacttacc
     3721 tgttttcccc agcaacgcgc cgagctttgc ccccagcagc ttcccgcttt tccccagcag
     3781 gttccccagt ttgccgagtc tcgccagatt cttcagtttt cccagcagtc ccgctggcgg
     3841 cagaagatag ctggctatcg ttattccccc caggatcatt ctctgccacg gcgggaactc
     3901 cggctggata tccagatagg tcacctgctc accgccaata aagacgtccg gcgaacctcc
     3961 cacgattgct gcgctgcact ccagtttgtc gccctggcgt gcggcaggct gattgttgat
     4021 aaagacgttg ctgctgccct gggctatcct cggtggtttg ttctccttgt cgcagagcgc
     4081 cgtgtcctca ccggcttccg ggaaatccac atcttttttg atttcgccac gcccgcccac
     4141 catggtggca aactccatta ataccggacc ggcgggaaac agaagcgatg cgccggcgct
     4201 gacgcgatcc ataaaaccgg cattgctgtc cgtcagggtg ataacagcgt tcccccaggc
     4261 cttcgccagt ggtactgctt tttccgctgc cgattcaagc aggtcagtgg caatatcacc
     4321 ccatgagggt tccccctgtg ggacttcagg tattgctcca ggtgggggag gagggggaag
     4381 ggtgacggcg gccctggcag cgggctttcc gttaattttt acgttttcag aaccggtatt
     4441 gatggcgccg tccgtgtcac aaaaactgtt tatccatgac tcaagctggt tctgtttctc
     4501 ctccacataa tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc
     4561 ataagggccg gtggcacagg cggccataat caggcaacct tctgcgatgg tcactatcgc
     4621 ccccagcacg gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag
     4681 cctggcggcg ggggggccag acttttttga tgccagcatc tgtgcaatgg ctttgtcgtt
     4741 ggctgcccgt ttcatctggc tggcgaccat cgcctgctgt gacagcattc gcattgccag
     4801 cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct tcactcatgt gttatccctc
     4861 gtttatgtca gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc
     4921 tctttatcac ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga
     4981 aagtgaaggt cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc
     5041 cctgattccc ctcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct
     5101 ctgccagagt gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca
     5161 gcgccagcgt ttcctggtac agctctggtg tcagggttcc ctgtggaacg gcttcgcggg
     5221 taatgaccag gttgatgcca ctgttatctg tggacaccag gacctgcatt gactgatcct
     5281 gccattgaga cggcagttga atgtggcctt cattaaaagt gaactgcata aaatactccc
     5341 tgataatctg agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct
     5401 tcgacagcct gattaccgtc gattccccga cctgtagctg aatgccatcc ggcgtcagcg
     5461 caattttgct ttcctccacg gccagcaaaa tttccttaat cgccgtataa gcctgtatac
     5521 cgtcgccttc gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc
     5581 tgtcctgaaa gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt
     5641 tttccaccgt aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg
     5701 tttcgcggtg atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt
     5761 tcttctgcgc gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat
     5821 taaatccgct gcccatgtac gttttggagc ggatggtcat ctgcgtcttt gttcccggca
     5881 ggctgccggg ggtgcggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg
     5941 gatcgccgtt gaggaagtct acaatcacct cctgacccac acgcggtatc gccaggtggc
     6001 caaaaccggt gcctgcccac gcctgtgcca cacggatcca gcacgaactg tcttggtctg
     6061 ccgggttata acggtcccag ttgaacttca cccgcacgcg accatgttcg tcgcagaaga
     6121 tttcctcgcc tgccggtcct gtcacgacag cgctctgcgg gccgtcgacc agcggtttca
     6181 gcaacggctg tggtcgccat gttctgtctg ccgggatcac cgcaaaatgg ttctccagcg
     6241 ccgtccccgc tccctgccgt cctggcaccg cctgtggctg ttcgccgtgc agttcacttg
     6301 ccaccacctg ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgcc
     6361 gtcccggcca tatctccggc gaacggcttg ttccgcgcgc cacttctgcg ttgtttcgcc
     6421 agccgtccat ctgccagcgg gcaaagttct gcccgtgggc acccttgaaa cgtccggggt
     6481 agtcatacac ttcatactgc gtgcgctggt aatcctggta ctggccttcc tgatcaaaac
     6541 gccctggcca gccgggtcgt ttaaaggtgt agtccttggt caccacggaa gaagggcgga
     6601 tttgtgcgct gtagcggaac tggctgatgc agagggtgct cacctcggta cgggtgttcg
     6661 ggttccatgg gatttcaaaa gattcgggca gatggcggac tgtgtcgcac agcaccaggc
     6721 tctggtcggt acttttgtaa gc
//
LOCUS       DAPPNJ010000218         6694 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000218 DAPPNJ010000000
VERSION     DAPPNJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.220"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(96..581)
                     /gene="sixA"
                     /locus_tag="RPM73_003893"
     CDS             complement(96..581)
                     /gene="sixA"
                     /locus_tag="RPM73_003893"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDX6522194.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(784..2928)
                     /gene="fadJ"
                     /locus_tag="RPM73_003894"
     CDS             complement(784..2928)
                     /gene="fadJ"
                     /locus_tag="RPM73_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDX6522195.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2928..4238)
                     /gene="fadI"
                     /locus_tag="RPM73_003895"
     CDS             complement(2928..4238)
                     /gene="fadI"
                     /locus_tag="RPM73_003895"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDX6522196.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSHRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4418..4702)
                     /locus_tag="RPM73_003896"
     CDS             complement(4418..4702)
                     /locus_tag="RPM73_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDX6522197.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5074..6414
                     /gene="fadL"
                     /locus_tag="RPM73_003897"
     CDS             5074..6414
                     /gene="fadL"
                     /locus_tag="RPM73_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDX6522198.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1654 a         1817 c         1595 g         1628 t
ORIGIN      
        1 agagtcttcg atagtagtga ggttgacaga tatccgctgc ctgatgagca attatgccca
       61 atgaatctac ctcattgggc ataatttgga actcatcaga tagcttttgc catcttcaga
      121 ttacacgggc tcatctgcca gttgaatgtg ccgtttccgc tttcatcaag cgttacgctg
      181 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
      241 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
      301 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
      361 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
      421 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
      481 atcaggcgag attcgtcaca accattagtg gtcagaggac gaacggaatc actggcggca
      541 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
      601 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
      661 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
      721 tatgcgttta cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
      781 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
      841 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggcaagtc gttgcattat
      901 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
      961 cggaaaacca atgccaaata ccgcgccaat atccccgtca cgcacgctac ggataacctg
     1021 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
     1081 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg caggatcgac
     1141 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
     1201 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
     1261 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
     1321 caaaagttgg attggaccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
     1381 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
     1441 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
     1501 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
     1561 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgt
     1621 ggcgtgagct gcgatatcac caatcggtaa agatgacgta ttcgaagcaa aaatggtatg
     1681 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
     1741 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
     1801 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
     1861 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
     1921 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
     1981 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
     2041 cttcacttcc gtactggcaa aaaagatact acgcaaagct tgcgattgtg gcgtcatcgc
     2101 cagttcgcca aacgcccgag cttcggcgtc ataaccgctg ctggtgccct gcgctaatcc
     2161 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
     2221 tgttttctta ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
     2281 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcgac
     2341 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
     2401 cgcccgaagt tgttttccgg tgaggatcat ctctaatgct gtgctgacgc ctatcagacg
     2461 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
     2521 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccaactccag
     2581 cccaccaccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
     2641 ctccgccatc aactgttggc cctgccgcgc cagagcttcc gcttcttgcg ccgttttgca
     2701 gttgccgatc atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa
     2761 caccacgccg cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
     2821 ggcaaattcc gccttcaggg tattcatttt ctcatccggt acgtcgatgg tgatgatagc
     2881 aatgttgtcc agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc
     2941 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
     3001 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag
     3061 ggatgcccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
     3121 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
     3181 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag atcgctcatt
     3241 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ccggaccgag caacatgtcc
     3301 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
     3361 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
     3421 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
     3481 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
     3541 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgcgcg
     3601 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
     3661 ttcgccattt gctctgcggt gtcacccatc cgcaagccgg tagaatattc tgctactgca
     3721 ggcggtacgg gcattaaatc gcgcaaacgc aggcgagaga agagtttcag gcgatggctc
     3781 atggttcgag ctttgttgac atcaaccagc acgcgcgcca gttttttact gacgccaatt
     3841 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
     3901 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
     3961 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
     4021 gcttcaggca tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc
     4081 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
     4141 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
     4201 tcgccctggc gggtaaccag cggtaaaacc tgacccataa cactcccctg taaaaaaata
     4261 aataaagtgg tctgacctga tcatagtctt aaccattttt ttacatttag ccaagcggag
     4321 aaaaaggaaa gtgggagcta tgacacagtg aagacaggag aaagaaaaga aaacgcccct
     4381 gccagcaact gacaggggcg aacgtacccg agaggaatta acgcagaccc agctggaaga
     4441 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
     4501 cttcagtgaa agtcggggag attttgcacg gttcggattc cacgctacgg gctttttcag
     4561 tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg
     4621 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
     4681 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
     4741 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
     4801 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
     4861 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
     4921 aagatggaaa catacttgca acattccagc tggtccgacc tatactctcg ccactggtct
     4981 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
     5041 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
     5101 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
     5161 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
     5221 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
     5281 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
     5341 ggtcgtagcc tgaaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
     5401 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
     5461 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
     5521 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
     5581 ggtttcaacg ccgtctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
     5641 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
     5701 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
     5761 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
     5821 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
     5881 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
     5941 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
     6001 ccacagtggg cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg
     6061 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
     6121 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
     6181 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
     6241 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
     6301 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
     6361 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
     6421 gttcgcctgg ataaagtcac ctgcatagca ggtgacttta actcccccac ttcaccgaaa
     6481 gtagtgtcct cattgcttac ctcctgagtt ttgcaaacag cctgttggca gcttgccctt
     6541 tcaaatcaat aagcggtatc cacagacaaa cctgaaggga aaggcatatt ttcaggcgtt
     6601 ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg
     6661 ctattgtgcg ctcatacact caaattaaag atag
//
LOCUS       DAPPNJ010000219         6637 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000219 DAPPNJ010000000
VERSION     DAPPNJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.221"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            5..667
                     /locus_tag="RPM73_003898"
     CDS             5..667
                     /locus_tag="RPM73_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDX6522199.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            763..1221
                     /gene="mgsA"
                     /locus_tag="RPM73_003899"
     CDS             763..1221
                     /gene="mgsA"
                     /locus_tag="RPM73_003899"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDX6522200.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(1253..3307)
                     /gene="helD"
                     /locus_tag="RPM73_003900"
     CDS             complement(1253..3307)
                     /gene="helD"
                     /locus_tag="RPM73_003900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDX6522201.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            3430..3876
                     /locus_tag="RPM73_003901"
     CDS             3430..3876
                     /locus_tag="RPM73_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDX6522202.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            3886..6048
                     /gene="yccS"
                     /locus_tag="RPM73_003902"
     CDS             3886..6048
                     /gene="yccS"
                     /locus_tag="RPM73_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDX6522203.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(6011..>6637)
                     /gene="sxy"
                     /locus_tag="RPM73_003903"
     CDS             complement(6011..>6637)
                     /gene="sxy"
                     /locus_tag="RPM73_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDX6522204.1"
                     /translation="KSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVSD
                     GELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSL
                     DAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNS
                     LVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
BASE COUNT         1505 a         1783 c         1618 g         1731 t
ORIGIN      
        1 acctatgaaa accggcatcg tgaccacctt gattgcatta tgtttgccgg tatccgtttt
       61 tgccaccacg ctgcggcttt caaccgatgt cgatcttctg gtactcgacg gcaagaaggt
      121 ttccagctct ctgctgcgcg gtgcggacag cattgaactg gataatggac cgcatcagtt
      181 agtgtttcgc gttgagaaga cgattcatct ttccaatagt gaagaacggc tgtatatctc
      241 cccaccgctg gtggtcagtt ttaataccca gctcatcaac caggtcaatt ttcgcctgcc
      301 tcgcctggag aatgagcggg aagctaacca ttttgatgcc gcgccgcgcc ttgaattgtt
      361 ggatggcgat gcgacgccga ttccggtaaa gctggatatt ctcgccatta cctcaacggc
      421 aaaaacgatt gattatgagg tcgaggttga acgctataac aaatccgcga aacgcgcttc
      481 actaccgcaa tttgccacga tgatggcaga tgacagtacg ctgctttcgg gtgtttccga
      541 gctggatgct attccaccgc agtctcaggt gctcacagaa caacggctga agtattggtt
      601 taaactggct gacccacaaa cgcgaaatac tttcctgcag tgggcggaaa aacaaccatc
      661 ttcctgagat ttttgtgcct gtgcgcaggc tttttcggtc tttatcttgc agcgataagt
      721 gcttacagta atctgtagga aagttaacta cggatgtaca ttatggaact gacgactcgc
      781 actttacctg cgcggaaaca tattgcgctg gtggcacacg atcactgcaa acaaatgctg
      841 atgagctggg tggaacggca tcaaccgtta ctggaacaac acgtactgta tgcaacaggc
      901 actaccggta acttaatttc ccgcgcgacc ggcatgaacg tcaacgcgat gttgagtggc
      961 ccaatggggg gtgaccagca ggttggcgca ttgatctcag aagggaaaat tgatgtattg
     1021 attttcttct gggatccact aaatgccgtg ccgcacgatc ctgacgtgaa agccttgctg
     1081 cgtctggcga cggtatggaa cattccggtc gccaccaacg tggcaacggc agacttcata
     1141 atccagtcgc cgcatttcaa cgacgcggtc gatattctga tccccgatta tcagcgttat
     1201 ctcgcggacc gtctgaagta atattgcaca ggtggcaaac gccacctgtt tcttacggtt
     1261 ttctcgccac cggcacatcc agatttttca gtatttccac aaagggagag ggattctctt
     1321 tgttaaacag tgcccatacc cgatggcgtg cccgggtcag cgccacgtac attaaccgcc
     1381 gttcttccgc gtccgggaaa tcctcaaccg gtggcagtag cgcctcttcc ataatcgatt
     1441 cccgcgccgc agccggaaaa ccatcacttc cctcctgcaa gccaacgatg atgacgtaat
     1501 ccgcctgttg ccctttgctg gcatgaatgg tcataaagtc gatttgcaac ttcggccagc
     1561 gtgttgccgc tttttccagg ctggcaggcc tcatgtgatg gtaacgcgcc aggatcagaa
     1621 tgcgctcttc cggtttggca taaccagaga gcttatccag caaagcgtca agttgactct
     1681 catccaataa cgtgacggct tttttgtctc cattggttaa gctgtttagc ggctttttca
     1741 gctggcctgg gttctgctga ataaaccggt ttgccacctt accgatacga ctgttaaaac
     1801 ggtaagtcgt gtctaaatca caacgttcgc cttcaccaaa gttttcatgg aaagcggtgg
     1861 tgagcgacat ttgcgcaccg ctgaatcggt aaatcgcctg ccagtcatca ccaacagcga
     1921 acaacgtcgt ctgactgttt tgcttgcgta atgccgctaa caacgctgcc cgctgcggcg
     1981 agatatcctg aaattcatca accagaatat gcttccacgg gctgataaag cgacctttct
     2041 ccagaatcac aatcgcctga tgaataaggc ccgaaaaatc gacagcgttt tctgccttca
     2101 gcgcaccttt ccaggctttt aataacgggg ccatcaactt gatacgttta ctgaacagat
     2161 cgcgaatctc ttcgggtgca ctggcaatca tttctgcctg tgcaccaccg tgcatccgca
     2221 tcagacttac ccagcgatcg aggcgagagg caaggcgacg ctgtaatttt tcatcatccc
     2281 agaagttacc ttctggcact gaccactgca tttcttccgt cagccattgc cgccagccct
     2341 tcgcctgcgc tttcttttcg ctgcattgct tgcgccactc agcaataaag agttcatgac
     2401 gggcagcggt atcattttcc agtttgctga ctatcggaac ttttttgctg ccctgctgaa
     2461 taatatgcag cgccagcgca tgaaacgtgc gtgcggtaat gtcttcggta tgtagccgtt
     2521 cgcgaatccg ctcgtccatc tcttcagcgg ctttgcgacc aaacgccagc aataaaattt
     2581 gctcagggga cgcttcacca cgcgccagca accagcctgc acgggccacc agcaccgacg
     2641 tttttccgct tcctgcacct gccagcacta acagagaatg ctcgccatta acgactgccc
     2701 gggcctgcgc cggattcagc ggtgaagact cgacctggcg gaaaaaatcc gcatactcgg
     2761 taagcatggc ttcggtatac gcctggttat gctgcaaccg agcgctttca atatctttca
     2821 accaggcctg acatttacgc cacgcctcac ggcagttatc gaattcttcc agtcggttaa
     2881 ccggcaacgg caacgccgac atagcctggc ggatttgttg ctgaacgcca gaggtttgct
     2941 cacgcgtcag ccatttattt tctccagtgc gcgtggcaat caaatccagt tgctggcgta
     3001 aaacaccaga cgcaatttcg ctcatctcgc cactccaccg ctgccagtga gcatcaagat
     3061 gatggtaaaa acgctgcgtc tcgccccatt cggtgccgtg cagacgcacc actttttcgt
     3121 ccggcagtac aaattccagc tcgccccata ccagaccgcg cttacagtga atcgccagta
     3181 attgattgaa aggaataagg tattcgtggc gatcgccgga gactttaatc ccggcattga
     3241 ggatcaccgc ccgatcgtaa gggtgctgtg ccagacgttt tccaagcgtt gtcgctttca
     3301 gttccatgac tctgtagatc cacacgaaat tatttcttat cagtgtaacc gtcagagaaa
     3361 acgtgctcca gcccaaaaaa acgttacaat tgccgcccat tactgaaaac cacagtaaag
     3421 cgaggtttta tgcgtaccgt tttgaacatt ctgaactttg tgcttggcgg atttgccacc
     3481 actctgggct ggctgttggc gactctggtc agtattatgc tgatttttac cttaccgctg
     3541 acacgatcct gctgggagat cactaaactg tctctggtgc cttatggcaa tgaagctatt
     3601 catgtcgatg aactgaaccc ggctggcaaa aatgtgctgc tgaatactgg cggtacggta
     3661 ttgaatattt tctggctgat tttctttggc tggtggttat gcctgatgca cattgcaacg
     3721 ggcatcgcac aatgtatttc aatcattggc attcctgtcg gcattgcgaa ctttaaaatt
     3781 gccgctattg cactatggcc ggttggtcgt cgcgtggtat cggtagaaac ggcgcaagct
     3841 gcgcgtgaag ccaatgcacg tcgtcgtttt gaataatcca ctaatatggc ctttatgcta
     3901 agtcctttgc tcaaacgcta tacctggaac agcgcctggc tgtattacgc gcgtattttt
     3961 attgcgcttt gtggaaccac agcgtttccg tggtggctgg gtgatgtaaa actgacgatt
     4021 ccgctaacgc tggggatggt ggcagcggcg ctgaccgatc tcgatgaccg actggcggga
     4081 cgtttgcgta acctcatcat tacgctgttc tgctttttta tcgcctcggc ctcagtagaa
     4141 ttgctgtttc cctggccctg gctatttgcg attggcttaa cgctctctac cagcggcttc
     4201 attttgctcg gcggtctggg tcaacgctat gcaacaattg ccttcggtgc attgctgatc
     4261 gccatttaca ctatgttggg aacatcactg tatgagcact ggtatcagca gccgatgtat
     4321 ctgctggccg gtgccgtctg gtacaacgtc ctgacactta ttggtcatct gctgttcccg
     4381 gtccgcccgc tgcaggacaa cctggcgcgt tgctatgaac aactggcgcg ttatcttgag
     4441 ctcaagtcgc gcatgtttga tcctgatatt gaagatgaaa gccaggcacc gctgtacgat
     4501 ttggctctcg ccaacggtca gctgatggcg acattgaatc agacgaaact ctcgctgctg
     4561 acccgcttac gtggcgatcg tggtcaacgg ggaacgcgtc gcacgctgca ttattacttt
     4621 gtcgcacagg atattcacga gcgtgccagc tcttctcata ttcagtatca aacattgcgt
     4681 gaacattttc gccacagcga cgtgctgttc cgttttcagc ggctgatgtc gatgcagggc
     4741 caggcgtgcc agcaactgtc acgctgtatt ttgctgcgtc agccttatca acatgatccg
     4801 cattttgagc gcgcttttac gcatattgat gctgcgctgg agcggatgcg cgataacggc
     4861 gcgcctgccg atttacttaa aacactggga tttttgctga acaatttacg cgccattgat
     4921 gcccaactgg caacaattga atcagaacag gcccaggcac tgccccataa taatgacgaa
     4981 aatgagctcg ctgatgatag cccgcacgga ttgagtgata tctggctgcg tcttagccgt
     5041 cacttcacgc cggaatccgc cctcttccgt catgcggtaa gaatgtcgct ggtgttgtgc
     5101 ttcggctacg ccatcattca gataaccgga atgcatcacg ggtattggat cttgctgaca
     5161 agtttgtttg tctgccagcc aaactataac gccacgcgcc accgcctgaa gttaaggatt
     5221 attggtacgc tggtcggtat cgccattggc attcctgtgc tgtggtttgt accgtcactg
     5281 gaagggcagc tggtgctact ggttattacc ggcgtgctct tttttgcctt ccgtaacgtg
     5341 caatatgccc atgcaacgat gttcatcaca cttttggtgc tactgtgttt taacttgctg
     5401 ggtgaaggtt ttgaagtagc gttacctcgc gtaatcgata cgctgattgg ttgtgccatt
     5461 gcgtgggcgg cagtgagcta catctggcct gactggcagt ttcgcaatct gccgcgaatg
     5521 ctcgaacgcg ccacagaggc caactgccgg tatctcgatg ccatactgga gcaataccat
     5581 caggggcgtg ataaccgtct ggcgtatcgt attgcccgcc gcgatgcaca caaccgtgat
     5641 gctgaactgg cgtcggtggt atcaaatatg tccagcgaac cgaacgttac cccgcaaatt
     5701 cgcgaagccg cgtttcggtt gctgtgcctt aaccatacgt ttaccagcta tatctcagcc
     5761 ctcggtgctc accgggagca gttaactaat cctgaaattc tggcgtttct tgatgacgca
     5821 gtttgctatg ttgatgacgc gttacatcat caacctgctg atgaagaacg cgtcaatgag
     5881 gcattagcta gcctgaaaca gcggatgcag caacttgaac cacgggcaga cagcaaagaa
     5941 cctctggtcg tacaacaagt tggattattg attgcattac tgcctgagat tggtcgtctg
     6001 caacgccaga ttactcaagt tccgcaggaa actcctgttt cggcgtaaga gagtcagccc
     6061 attctgcaag ctcctggcgg cgtgccaccg ggagcgcagc ttcatgaatg ccgataatgg
     6121 caccttcaag cataaacaga atcttttctg tcaccagact gttttgctgc cgcagtcgca
     6181 accaacacat ttttgcccca agtatacgta acgcccgtac atccttaatc cccacctccc
     6241 cgagaatcgc ttccagatga aaagacatat tgggcaaatc tttcagtctt tcccgggtat
     6301 tgcgcgtgct tttctctttc agcgctgcat cgagagaata tttcgacaga cgcaccagct
     6361 tcagttgatt tcgccataga ctttcatcaa cccgatagta attgagggta acggatcggc
     6421 cacacttttt atatgtcagc cagacaggcg gatgttttac acagtactgt gcactttgct
     6481 cacaagcccg aagatacaac tcaccatcag aaaccatcgc aaacaccgtg tcgtcaacgg
     6541 tcaggctgta actgccaaac aatgatcggt attcaattgt gcccaacgtt gccaggtatt
     6601 cttgtgattt atagatccgc ttataggaga ggctttt
//
LOCUS       DAPPNJ010000220         6598 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000220 DAPPNJ010000000
VERSION     DAPPNJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.222"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            122..364
                     /locus_tag="RPM73_003904"
     CDS             122..364
                     /locus_tag="RPM73_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522205.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            478..1116
                     /locus_tag="RPM73_003905"
     CDS             478..1116
                     /locus_tag="RPM73_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDX6522206.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1113..1850
                     /locus_tag="RPM73_003906"
     CDS             1113..1850
                     /locus_tag="RPM73_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDX6522207.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1850..3946
                     /locus_tag="RPM73_003907"
     CDS             1850..3946
                     /locus_tag="RPM73_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDX6522208.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFYFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3993..4271)
                     /locus_tag="RPM73_003908"
     CDS             complement(3993..4271)
                     /locus_tag="RPM73_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522209.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4541..4951
                     /gene="psiE"
                     /locus_tag="RPM73_003909"
     CDS             4541..4951
                     /gene="psiE"
                     /locus_tag="RPM73_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDX6522210.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="RPM73_003910"
     CDS             complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="RPM73_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDX6522211.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1624 a         1680 c         1774 g         1520 t
ORIGIN      
        1 tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta gttgtgtata
       61 ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg
      241 gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct tgaagtatga
      421 agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg
      481 aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa
      541 gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac
      601 gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcaa
      661 ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa
      721 gccactcttg tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg
      781 attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt
      841 gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca
      901 cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga tgaaaccccg
      961 gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctactgg
     1021 atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg
     1081 gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt gtcgcattga
     1141 ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta ttcagcaatg
     1201 gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg gtaggccagc
     1261 cgcggctggc aaacagttgg tggcctggtg cggtgattag cgaagagctg gcaacggcgg
     1321 cagcattgcg gcagcagcag gcgttgctga cacggctggc agaactggct gcagattcca
     1381 gcgccgacga tgccgctgcg attaacgcct tacgccagca aattcaggcg ttgaaagtga
     1441 cgggcaggca aaaaatcaat cttgatcccg atatcgtccg cgttgccgaa cgcggtaacc
     1501 cgccgttgca gggcaactac acgctgtggg tcggaccacc gccgtccacg gtcacgttgt
     1561 tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgtgac gtggcgagct
     1621 atctctctga ccagagcctg ctcagcggtg cggatcgcag ctacgcgtgg gtggtttacc
     1681 cggacggacg cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga
     1741 tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag acgcctgatg
     1801 cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa tgaaaaaaag
     1861 acatctgctt agcttactgg cgctgggcat tagcatggct tgctacggcg aaacatatcc
     1921 tgcgcccatt ggcccgtcgc agtcggattt cggtggcgta ggattattac aaactcccac
     1981 cgcgcgtatg gcacgggaag gggagttgag tctgaactat cgcgataacg atcagtaccg
     2041 ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga
     2101 cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga
     2161 taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc
     2221 ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag
     2281 caaagcctgg gggccgttct attttacgct cggcctgggc tggggttatc tgggcaccag
     2341 cggtaatgta aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag
     2401 ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt
     2461 tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg
     2521 caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg
     2581 tgcgatttat cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac
     2641 ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga
     2701 taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt
     2761 ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc agatccaggc
     2821 gaaaggcgat acgctgtatg tcaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg
     2881 gatcatccgt gctaatcgga tcgtgatgaa cgatctgccg gatgggatca aaacgatccg
     2941 cattacggaa aatcgcctta acatgccgca ggtgacgacg gaaaccgatg tcgccagcct
     3001 gaaaaatcat ctcgccggag agccgttggg ccacgaaacg acgctggcgc aaaaacgcgt
     3061 cgagccagtg gttccgaagt ccaccgagca gggctggtat atcgacaaat cacgctttga
     3121 tttccacatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta
     3181 tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctgc tgaccaccgg
     3241 cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacta atcctccgca
     3301 ggactcgcac ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta
     3361 tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt
     3421 ctacggtggt tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgccc
     3481 gctggacagt aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg
     3541 tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc
     3601 gtactggacg ccatctttcg ctcaggatgt gttagtgaaa gcgagcgtcg ggcagtatct
     3661 ggcgggcgat aaaggcggca cgctggagat cgccaaacgc tttgatagcg gcgtggtggt
     3721 tggcggctat gccacgatca ccaacgtttc gaaagaggag tacggcgaag gggacttcac
     3781 caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga cacgcagccg
     3841 tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt
     3901 ccagttgtat gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt
     3961 gccagttttc gcactcactg gcaccctcat tcttaatgac ctgttgagcc atcaggaacg
     4021 cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc
     4081 gtcgatatcg gttgcagatc tggttgcctg agcgactggg ccatcattgg gctggagaca
     4141 ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga
     4201 acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg
     4261 ttacgtttca tttgttccag aggaacatgg tcgatttttc gcgcattgct ggtggctggg
     4321 aatcacctga atgggtgatt tttgaattac cagcttgggt gcggtttgtc ttgccggatg
     4381 cgccgccagg cgcggcttat ccggcctacg ggtaggtata tccggctttg gtggaggcgc
     4441 gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg
     4501 cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc
     4561 gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc tgctgtgcct
     4621 cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggcag atgtgctgtt
     4681 cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt actttctcta
     4741 tttcgaattt atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg
     4801 ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc
     4861 gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca ctctgtggct
     4921 gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt
     4981 gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag
     5041 agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat
     5101 agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg
     5161 gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc
     5221 agccactggg ccgccaccgc gattgccagc gctttaccac gaatagcatt cgggaagatt
     5281 tccgacagca gtacccagca taccggaccc caggacatgg caaaggcggc aacatagaac
     5341 agcatcgaca gtagcgccac aatacccgat gcctgagtgt aaaacgcggt accgaggcta
     5401 aacataccga ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta
     5461 tccaccgtca taattgccag tacggtaaag gtgaggttga taactccgac aataatggtc
     5521 tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac
     5581 agcaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc gattacaatc
     5641 acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt
     5701 ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatactt
     5761 tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac
     5821 agcagcatta agaacagcag tgcagggata cattccgagg caaacatata acgccagccg
     5881 tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac gcagtaaact
     5941 aaaagttgcc cgaaaataat cgcaaactgg ttaaatgaaa ccagtttccc gcgaatatga
     6001 gctggagcca gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata
     6061 ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata aacaggcact
     6121 gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa
     6181 ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gttactgcaa
     6241 taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggctac gcaaaaccct
     6301 aacagggagt tggcagcgga ttcatttaag ttttgtggag caacaaagac ggtattgagt
     6361 gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta
     6421 gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc
     6481 cttagatcat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc
     6541 aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgat
//
LOCUS       DAPPNJ010000221         6426 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000221 DAPPNJ010000000
VERSION     DAPPNJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.223"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            34..966
                     /locus_tag="RPM73_003911"
     CDS             34..966
                     /locus_tag="RPM73_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6522212.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(1008..2096)
                     /locus_tag="RPM73_003912"
     CDS             complement(1008..2096)
                     /locus_tag="RPM73_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX6522213.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(2238..6221)
                     /gene="ehaA"
                     /locus_tag="RPM73_003913"
     CDS             complement(2238..6221)
                     /gene="ehaA"
                     /locus_tag="RPM73_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HDX6522214.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAIISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLVLNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         1571 a         1716 c         1381 g         1758 t
ORIGIN      
        1 aaattcatat tagataatgg cgggaatggt ttaatgaact cagtttttac cgaagagaat
       61 ctgttggcat ttactaccgc tgcgcgtttt ggcagtttca gtaaagccgc agaagagttg
      121 ggtttaacca cttccgccat tagctacacc atcaagcgaa tggaaacagg gctggatgtg
      181 gtgctgttca ctcgcagtac ccgcagcatt gagttaacgg agtccgggcg ttattttttc
      241 cgcaaagcta ccgacctgct gaatgatttt tatgccatca aacgtagcat agatactatt
      301 tcgcagggca tagaagcgcg tgtgcgcatt tgtattaatc agctgcttta tacgcccaaa
      361 cacaccgcgc gcttattaca ggtgctgaaa aagcagtttc ctacctgcca gattaccgtc
      421 accaccgaag tgtataacgg cgtctgggat gcgattatta ataatcaggc caatatcgcc
      481 atcggcgcac ccgatacact actggacggc ggcggtattg attacactga aattggcgcg
      541 attcgctggg cttttgctat cgccccggat cacccgttag cctttgtgcc ggaacccatc
      601 gccgaaagcc aactgcgtct ctaccctaat attatggtgg aggacaccgc gcatacgatt
      661 aataaaaaag tgggctggtt gctgcacggg caggagtcaa ttctggtgcc agatttcaac
      721 accaaatgtc agtgccagat tttaggtgaa ggaattggtt ttttgccgga ttacatggtc
      781 cgtgaggcga tgacgcaatc cctgctggtt acccgacaaa tccataatcc gcgccaggat
      841 tcgcgcatgt tactggcgac tcagcattct gctacgggtc aggtgacgca gtggattaaa
      901 aaacaatttg cgccaaatgg catattaacg gggatctatc aggatttact gcatcgggag
      961 aattaatcgc tgccaacctc ccgatgcgct gtgaatagcg gtttacatca accacctgct
     1021 ttcattaccc attcagagat aaatttatta cccggtactg gcggagagta caaatagccc
     1081 tgcaaaaagt gaacgccttt accgatcatt aaatccgcct gctcctgggt ttctacccct
     1141 tccgccacga tactcagacc aggcttacgc gctagttcga caatattgtc cacaatatga
     1201 ccggagattt cgtcgacact cgccatttgc acaaatgact tatcgatctt aataaaatcg
     1261 accgggaacg cctgcaagta acgataggtc gcataacccg taccaaagtc atccagcgca
     1321 aaggtaatgt tgtgctgatg aaggctgtca aatatcgctc tggcttctgg cgttaccgga
     1381 atagggttac gttccgttag ctcgagaacc agcttgattt tatcgttacc taatttctta
     1441 accaggttca gacactcttt ttcaaatccc gctgccaaaa aacaacccgc cgagacgttg
     1501 atgccaatat ggaaattgtc cggcagcaaa tgttttaccg gcatcagaat atccgcagtc
     1561 tgtttcatca gttggcgggt cattatgaca ataagaccgg atgactccgc cagaggaata
     1621 aactgatccg gtgggataat tcccgtttgt ggatgttccc agcggacaag tacctcacag
     1681 cccgtcagta ccccagtctg cgcgcagaaa accggttgga tccacggttt gaattcgtgg
     1741 ttttccaacg ccagactgat ggcttcaggc gtgacgatat ggtgacaacg gttatcaggg
     1801 atataaaaat tatttttatt tcccgtgcca ctgatgactt gcggatgata ttgaaaaaag
     1861 atatcccgcc agaaggtaat atcactctgg atgcctagtt tttcatacag ctgcttcttt
     1921 tgatgtgaaa ttgtctttgc actgcgattt ctgtactgtg agatttctgt aaccgacatt
     1981 ccctccatta gcagcttgag taaaagccgt tctttttcgg acacgatacc aggcaacgtc
     2041 aaattcaccg ttgccccgaa ctcttccaaa aaaacactca aatcatgcga agccatcgaa
     2101 cacaccttga ttaaatcttg tgcgccagct aaatctgcga gtcttcactc tgactgataa
     2161 agctggctgc aagaaaatga cacagagagc cgaattatct cccgttcggc tctctctcct
     2221 tttactcatt attcagatca gaattgccac ttaatgccaa ccattgcaga ggtgtcatta
     2281 tatcccctat cggcaacctg gacgcccaca ttcccccaga cattcaggtt ggcatttagc
     2341 tgtccttcca ccccggtttt tatctcagca atatttcggg ctccatcctg agtgacggac
     2401 acgccatcca tactggtgct gaaatcctta ctgttatgca gccagttcac ttctacaaac
     2461 ggctggaact cgcgggattt accgtcatcc attttatggt ggctcttcag ccaggttttt
     2521 acgccaagtc gggtttgaac attgccatca ccgttgctat gaacgagggt tccgttgctt
     2581 tcgcggtgct tatcggcttt gactcccatc caggtaacct gtgcttgcgg ctgaacatac
     2641 cattcattac gcgttccctg gctgccatta aattcagcta atttgtgttt gtatccagct
     2701 tccagtgaag cggtaaatcc ttttgattta taggattcac tttgtaagtc atcccctttc
     2761 actgtgttat caaaccagct gtactgcgcc caactgtcga gatacgcgcc attacgcgac
     2821 tcgtcatcgg cataccaggt ggcatagagg ccggtgctat atccgttcac actcgctttt
     2881 gcacgataac cggttcgcga ggaaatggtt ttgctgtcgc tgttgccata tcccgccatg
     2941 accccaacat gccagctgtc gctgccgttt tggctccact gcgcgacatc gcctcccagt
     3001 tgcagaacat agcgattgct ttgggttttc agctggccgc tgccatcacg ccatttatta
     3061 tgaccacctt catggcgcat ccacatagtg gtttgtttct gctcacccgt caccatgtcg
     3121 gtatagtacg tattacccag acgctcatgc agacgcgtgg tgaacattgt attcgctgct
     3181 gcaaggttcg caatgtagct acccgcctcc ggtcgcaggt cattatccac attcagatcc
     3241 ggacccggcg taggtgtcgg gttagggttc ggctctggat tcgggtttgg ctccggattc
     3301 ggcatcgggt ctggctccgg ctgcagttca ggagaatcac tgccgctggt cagataccag
     3361 ttaccactat ttgccccttg tccacgcgcg agagtgtagt catacgcccc cgcaacgata
     3421 cgaccagcct gaacaaattc gccctcagac ttaccgtcta catggataag ttcgataccg
     3481 ttaagggttt tcgcacctgt accgccagcg ttattcaccg taacggcagt cgtgccgcta
     3541 gtatcgccct cgacaaccaa tttatcggtt actgagtcat cgccattcag taccgtattc
     3601 attactagtt gaccgttatt accgtggtag ttccccttca ccactaacac attgccagct
     3661 tgctgtcctg acattccggt catcagcgta ccgttgttgg tcagattgcc gcccagggtg
     3721 taattcacag acgcagccgc tcttttttca gcgctattat tcaacaccag ggtaccgttg
     3781 ttctcaacgt ttgcgctacc caatccctgc gcatgactta ccaggacatt agccgaatta
     3841 gcaatgttca cattcgccgt caggttgctg ttatcgcccg cgatgtccag cgtgccgccc
     3901 tgaatattca gctctccgct ccccgttaac gaaccggttg aagtgccccc gttggtaacc
     3961 gtcagactgc cgccatttaa gctcagcagt gaatcggcgc tgccattgag cgtgcccacg
     4021 gtctggctgt gaccattcat atccagttca gtctctgccg ccagacgcag ttcatgggta
     4081 ttacccaaca cgttatcgtt attcaacaaa agcgtcccac tgcgcagatc ggtaacaccg
     4141 gtatagtcgt tgtctttgtt agaaagcgat acggtctgac ccttctggct gctgaatgcc
     4201 aggtcaccac tgccggtaat ctttgcgctg agatcggcgg cattctcgct tttaccgtta
     4261 gagctcagca ccagagcatc gctgtcggta gcgtgtaaat caagctgggt caggccgtaa
     4321 ccgatataca gaccgtcatt gtttacccca ctcgtcagac gatagtcgta agtgccgacg
     4381 gccgcttttt gcccattctg ctgaatatca cgttgggcac tgctggagag gatttgccca
     4441 tttttatcct gcaattgcag attgcccgca tcgcccagaa cttccgcacc ttgcgcatca
     4501 accaacttaa tggtgctgtt accatcatcg acttcagtga gcgataacgc agagtcaata
     4561 tcacgagata ctgagcttac aacgtcactg tcagaaacct gaatcacacc ctcgccgcgc
     4621 aaatccagcg ttttactaac gttgaccgtc ccttctgtca tctgtgcacc tgctgtcagg
     4681 gcaccaaaat caacggttcc gccggagaat cccaaagtgc taatggagtg agcgccctta
     4741 cccactgtta ccaggcttcc ctgacccgca gtgatcccgc ctctctgcag aaactcagca
     4801 ttcttttcca ggttaaatgt cgtgttctgg aacgccagtg aaccctggaa ccagtctgta
     4861 taagcaaact catcgaagga gaattcgtga ttccccatgt ttatttcaac aatacctcta
     4921 ccttgaagat cgttatcaaa tacccaatct ttattggcat tgatttgcag tttgccatca
     4981 acatccaggt ccgcacggcc gatgttcttg tcttcagaaa taatcgcgac agcaccagaa
     5041 gcgacatcaa tatatccgct gaatgcatag ttattgccgg tcagtgccac tgtcccactg
     5101 tcgatactta ttgtaccatt gccggaaatc ttattggcaa aggtcatgct atcgtcagcc
     5161 ttcaggtgta atttcccgcg gttgttgatc tctgctgtac ctatattttt ggtgatccct
     5221 aaagcgccat tttgctcaat tttgacttta ccactaaagg cagagttatc accggttaag
     5281 taagtcgtgc cattgagcgc attgatatag cccaggccct cgctgccact gacaatatcc
     5341 gcggaaatag tcgcgttttt atccgtgtgg tttaaggtga ttttcttatc ggatgtcttt
     5401 gcccacacaa actcaatttt ttcggcatca ataatccctg cagccttcgc agcacttcct
     5461 tcctgtgcac ctaacgctaa ctcagagttg gtgcttaaac taattttcgg tgcggatatt
     5521 ttacctccat ctgatacgac taatgttgta tcagaacgtt ggtcgtaata acccaggcta
     5581 atatgttcac gagcattaac ttcaccaccg ttattaaccg acaggaactg attaccgcca
     5641 tatccgccgt aaacgcccat aaagaaggtc cctgcattta cctgactacc ttcgccgctt
     5701 acattcccct gcgtataagt cagacgactg gtgcccttaa catgaatttt gccgccattg
     5761 gagacattaa aatagccttg atcatgtctc atgcctgagt ttgactcata caaatcatca
     5821 gcatataact cgctgccagc attggagaca ttcaggaacc ccgtaccttt cagcaaaaag
     5881 ttaagcgggc cattgattgt tacctttcct ccggtaatat ccaacccacc accagaagcg
     5941 agagtcagac gcggacggga agtatctgtc gtattgatta ccagattggc attgtcagtc
     6001 accgtaatac ggtaatcgat gctctggttc tctttgccaa aatcaatagc tttatcctgg
     6061 tttacgatga catcatcggc gaataccaga gatgaatata aacttatcgc gctaattaaa
     6121 acagcccggg acttttttcc ttttactctg ctccccagct ccgagcagac gctccaacag
     6181 ttacgggacc tgttccactt tagccgatat attttattca tcacaatctc cagcaataca
     6241 tccaaataaa accaagattg cataaacagc aacgcattaa atgccattct ggttactgtt
     6301 catcgcatat aaaaattcaa aaattttgca tagcattggc aaccacatat ctgagtatgt
     6361 agttcagagg gtaataaaat agcaagttac tactgaaaac ttattttaag ttaacactct
     6421 taactt
//
LOCUS       DAPPNJ010000222         6423 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000222 DAPPNJ010000000
VERSION     DAPPNJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.224"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            17..745
                     /gene="gadW"
                     /locus_tag="RPM73_003914"
     CDS             17..745
                     /gene="gadW"
                     /locus_tag="RPM73_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDX6522215.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1108..4221)
                     /gene="mdtF"
                     /locus_tag="RPM73_003915"
     CDS             complement(1108..4221)
                     /gene="mdtF"
                     /locus_tag="RPM73_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HDX6522216.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIITTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4246..5403)
                     /gene="mdtE"
                     /locus_tag="RPM73_003916"
     CDS             complement(4246..5403)
                     /gene="mdtE"
                     /locus_tag="RPM73_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDX6522217.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5463..5741
                     /locus_tag="RPM73_003917"
     CDS             5463..5741
                     /locus_tag="RPM73_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522218.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5742..6269)
                     /gene="gadE"
                     /locus_tag="RPM73_003918"
     CDS             complement(5742..6269)
                     /gene="gadE"
                     /locus_tag="RPM73_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDX6522219.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1599 a         1571 c         1576 g         1677 t
ORIGIN      
        1 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
       61 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
      121 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
      181 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
      241 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
      301 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
      361 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
      421 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
      481 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
      541 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
      601 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
      661 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
      721 acattcgcca ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
      781 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
      841 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
      901 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
      961 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     1021 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     1081 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     1141 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     1201 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     1261 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     1321 ccgcgccgct tcgatgattg cctctatcgg cgttttccct tctttctgca tcatctcaac
     1381 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     1441 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     1501 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     1561 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     1621 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     1681 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     1741 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     1801 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     1861 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     1921 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     1981 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     2041 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     2101 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     2161 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     2221 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     2281 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     2341 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     2401 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     2461 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     2521 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     2581 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     2641 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     2701 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     2761 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     2821 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     2881 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     2941 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     3001 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     3061 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgt
     3121 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     3181 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     3241 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     3301 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     3361 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     3421 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     3481 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     3541 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     3601 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     3661 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     3721 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     3781 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     3841 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     3901 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     3961 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     4021 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     4081 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     4141 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     4201 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     4261 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     4321 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     4381 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     4441 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     4501 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     4561 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     4621 cgggtcggag aatttcagcg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     4681 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     4741 gcgtaagaaa tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     4801 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     4861 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     4921 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcaact gggtgcgcgc
     4981 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     5041 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     5101 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     5161 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     5221 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     5281 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     5341 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     5401 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     5461 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     5521 ggtatagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     5581 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     5641 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     5701 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     5761 cccagggtga cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc
     5821 tgaatcttta atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta
     5881 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     5941 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     6001 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     6061 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     6121 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     6181 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     6241 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     6301 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     6361 tataagaact ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     6421 tta
//
LOCUS       DAPPNJ010000223         6387 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000223 DAPPNJ010000000
VERSION     DAPPNJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.225"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(298..1968)
                     /gene="betA"
                     /locus_tag="RPM73_003919"
     CDS             complement(298..1968)
                     /gene="betA"
                     /locus_tag="RPM73_003919"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDX6522220.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1982..3454)
                     /gene="betB"
                     /locus_tag="RPM73_003920"
     CDS             complement(1982..3454)
                     /gene="betB"
                     /locus_tag="RPM73_003920"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDX6522221.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3468..4055)
                     /gene="betI"
                     /locus_tag="RPM73_003921"
     CDS             complement(3468..4055)
                     /gene="betI"
                     /locus_tag="RPM73_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDX6522222.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4184..6217
                     /gene="betT"
                     /locus_tag="RPM73_003922"
     CDS             4184..6217
                     /gene="betT"
                     /locus_tag="RPM73_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDX6522223.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1322 a         1740 c         1746 g         1579 t
ORIGIN      
        1 taataaaaag gataacacga acctttttct tcagataaaa taaaatctca atcagatcga
       61 tatcctgttt gatttgttca cgcataatat atccagaaaa taaaatctgt cgcagataag
      121 gttgtattaa tagtctgtat caggaatgtt cgggttaaat atcagcaaaa agccagcatc
      181 atgaatactg gatatgaagc atgagagtta cctcagtgtt tatataagga ttcggtcccc
      241 ctctctggaa cggtaactct caatctgatc ggttcctgcg ttagttcaca tcacgactca
      301 ttttttcgct ctcaccggca tcccatttgc cacaaaatat cccgccgtgc tcctcggcag
      361 cgcttcctgt ccacgaatca tatccgctat tttctcgcca atcataattg tcgtggcgtt
      421 caaattcccg gtgataatct gcggcataat cgacgcatcc accacacgca ggccttctaa
      481 cccgtgtacg cggccttcgc cgtcaaccac ggacatctcg tcgtaaccca ttttgcaggt
      541 accgcacgga tggaaggcgg tttcggcgtg gttacgcacg aactcatcga gctgttcatc
      601 cgtctgacat tccgtgccgg ggctgatttc gcggccacga tactgatcca gcgccggttg
      661 atgcatgatc tcccgggtga tgcgaattgc gtcgcggaac tcctgccagt cctgctcgtg
      721 tgacatgtag ttaaacagaa ttgccggatg ctggtgcggg tcgcgggatt taatccgcac
      781 atgcccacgg cttggcgagc gcattgagcc gacgtggcac tggaaaccgt gctctttcac
      841 tgcattcgag ccgttatagt taatcgctac tggcaggaaa tggtactgaa tattcggcca
      901 cgcaaattcc tcacggctgc gaataaatcc acctgcttca aagtggttgc tggcaccaac
      961 gccagtgccg ccaaacagcc actccgcacc gattttcggt tggttccacc actgcagggc
     1021 agggtagagg gaaaccggtt ctttgcactc atattgcaga tacatctcca gatgatcctg
     1081 aagattttcg ccgacgccgg gtaattcatg caccagcgga atatcaaact ccgccagcag
     1141 ttcagcgttg ccgacgccgg agcgttgcag gatctgcggt gaggcaatcg cgcctgcaca
     1201 taacagcact tctttgttgg ccgttgcgcg ggttgggatg gtgctgtcgc cttccagcca
     1261 ttcgacgccc accgcgcgtt tgccgtcaaa aatgatgtga tcggtcatag cgtgagtacg
     1321 aatggtcagg ttagggcgcg atttggcctg atcgagataa ccgcgcgcgg tactggcgcg
     1381 acggccctgc ggcgtgacgg tgcgatccat cgggccaaaa ccttcctgct gatagccgtt
     1441 gagatcgtcc gtgcgcgggt agcctgcctg cacgcccgct tcaatcatcg cttcaaacag
     1501 cggattgacg ccgggtttgg aggtggtgac gctcaccggg ccatcgccac cgtggtagtc
     1561 gttctcgccc acatcgcgag tctcggcctt gcggtagtag ggcaggcagt cgagataact
     1621 ccagttctcc agaccgggtt cttgcgccca gttatcgaga tccagcgcat tgccacggat
     1681 gtagcacatg ccgttgatca gcgacgatcc acccaggcct ttaccgcgtc cgcactccat
     1741 gcggcggtta ttcataaacg gttcaggctc tgtttcatag gcccagttgt agcgtttgcc
     1801 ctgtagtggg aacgccagcg ctgcgggcat ctgggtgcgg aagtcaaagc gatagtccgg
     1861 gccgcccgct tcaagcagca gcacggaggt attcggatct tcagtcagac gggtagcgag
     1921 aacgttgccg gctgagccgg caccaataat gatgtagtca aattgcaaat aaacctcctg
     1981 gttagaatat ggactggaat ttagccatct caacctggat ggacttcacc tgggtgtaac
     2041 tctggagcgt catcacgccg ttctcgcgac caatgccgga gtgtttgtag ccgccaacgg
     2101 gcatctctgc cggggattcg ccccaggtgt tgatccagca aatacccgct tccagctgat
     2161 gaatgacgcg atgcgcgcgg ttcaggtccg ccgtcacgat gcccgccgcc aggccgtagt
     2221 cggtatcatt ggcgcggcga atcacttcgt cctcagattc ataagtgaga agcgacatca
     2281 ctggcccgaa gatctcttca cgcacgatgg tcatatcgtc gctgcaatcg gtgaacactg
     2341 tcggtgcaac ccatgcgccg ttatcgaagc catcgccttt cagtacatcg ccgccgcaca
     2401 gtacgcgcgc gccttcctct ttgcctttgg cgatatagcg cagcacgtta tcgcgatgcg
     2461 ggaagctgac cagcgggccg aagttagttt gcggatcgaa aacgtcgccc gcgcgaatgc
     2521 gctcaacgcg cgccagaatt ttctgctcaa atgcggcttt gcatttcgcc ggaacgaaga
     2581 cgcgggtgcc attggtacac acctgaccgg agctgaagaa gtttgccatc atggcgatat
     2641 cggcggcgag atcgagatcc gcatcatcga aaacgatcag cggtgattta ccgcccagtt
     2701 ccatggtcac ttctttcagg gaagaggccg ccgagttagc catcactttt ttgccgctgg
     2761 cgacaccgcc ggtaaatgac actttggcaa tgcccggatg ctcggtcaga tattgcccgg
     2821 tctccgcgcc cacgcccggc aacacgttaa atacgccgtc cggcaggccc gcttcgctgt
     2881 aaatttcagc cagctttaac gcggtaagcg gggtaacttc gctcggtttg aaaatcattg
     2941 cgttgcctgc cgccagcgcc ggggcggatt tccacagggc aatctggatc gggtagttcc
     3001 atgcgccaat ccctgccact acgcccagcg gttcgcggcg ggtatacaca aaggacgttt
     3061 cacgcaacgg gatctggctg ccttccagcg ccgggatcag cccggcgtag tactccagca
     3121 cgtccgcacc ggtaacgata tcgacggttg aggtttccga atatgctttt ccggtgtcga
     3181 gggtttccag ttttgcgagt tcgtcattgc gttcacgcag aatatcaacg gcccgacgca
     3241 gaatacgcga gcgctccata gcggtcatcg ccgcccagat tttttgcccc tgttgggcac
     3301 tcttcacggc gcgatcgaca tcctcacgcc cggcggcctg cacggtcgcc agcacgttac
     3361 cgttggccgg gttaatggtc tcgaaggtgc gaccgctggt ggcggaggta taaccaccat
     3421 gtatataaag ctgctgttct gccattcggg acatcacgtc tcctcggtta atcggtgggt
     3481 aaatgctgag taataaagtg acgggtcagc gagtgtgcga gaggtttatc cagcggtttg
     3541 ccgctcagtg ccgcgcgcag ccataatccg tcaatcagcg cggcaaggcc gtagcccgct
     3601 tcctgtgcct gttggcgcgg taactcgcgg cgaaattcgc tcaccagatt cgacaataag
     3661 cggcggctac tgacctgctg taaacgatag agcatcggct gatgcatact gctggcccag
     3721 aacgccagcc aggctttcat cgccgcactg ctcacctgcg tttcatcgaa gtttccgcca
     3781 acaatcgcct gtaatcgctg ctctgcactg ccctgcggaa gtgcatgtaa tcgattcaaa
     3841 accgcgtcac gcagctgact ggtgatatcg cgcatggttg cttccagcag accatttttg
     3901 tccctgaaat agtggctgat gatccccgta gaaacgcctg cacggcgggc gatctgcgcg
     3961 atcgttgcat cgtgcatgcc cacttcattt attgcttcca gtgtggcgtc gatcagttgt
     4021 ctgcgccgga tcgactgcat ccccaatttg ggcattttcg ccactccatt catcagcgtt
     4081 gtttatctat taaagcggtt attgattgga cgttcaatat aaaatgtgtc ttaattgtta
     4141 cgaatttgat tttaaatggt aacaataaca gtggggatac tggatgacag acctttcaca
     4201 cagcagggaa aaggacaaaa tcaatccggt ggtgttttac acctccgccg gactgatttt
     4261 gttgttttcc ctgacaacga tcctgtttcg cgacttctcg gccctgtgga ttggccgcac
     4321 gctggactgg gtttctaaaa ccttcggttg gtactatcta ctggcggcaa cgctctatat
     4381 tgtctttgtg gtctgtatcg cttgttcgcg ttttggttcg gtgaagctcg ggccagaaca
     4441 atccaaaccg gaattcagcc tgctgagttg ggcggcgatg ctgtttgctg ccgggatcgg
     4501 tatcgacctg atgttcttct ccgtagccga accggtaacg cagtatatgc agccgccgga
     4561 aggcgcggga cagacgattg aggccgcgcg tcaggcgatg gtctggacgc tgtttcacta
     4621 cggcttaacc ggctggtcga tgtatgcgct gatgggcatg gcgctcggat actttagcta
     4681 tcgttataat ttgccgctca ccatccgctc ggcgctgtac ccgatcttcg gtaaacggat
     4741 taacgggccg atagggcact cagtggatat tgcagcggtg atcggcacca tcttcggtat
     4801 tgccactacg ctcggtatcg gtgtggtgca gcttaactat ggcttgagcg tactgtttga
     4861 tattcccgat tcgatggcgg caaaagcggc actgatcgcc ttgtcggtga taatcgccac
     4921 gatctctgtc acctccggtg tcgataaggg gattcgtgtg ttatcggaac ttaacgttgc
     4981 gctggcgctg ggattgatcc tgttcgtatt gtttatgggc gacacctcgt tcctgcttaa
     5041 tgcgctggtg ctgaatgttg gcgactatgt gaatcgcttt atgggcatga cgctcaacag
     5101 ttttgccttc gaccgtccag ttgagtggat gaataactgg acgctcttct tctgggcatg
     5161 gtgggtggca tggtcgccgt ttgtcggctt gttcctggcg cgtatctcgc gtgggcgtac
     5221 cattcgccag ttcgtgctgg gcacgttgat tattccgttt accttcacgc tgttatggct
     5281 ctcggtgttc ggcaatagcg cgctgtatga aatcatccac ggcggcgcgg catttgccga
     5341 ggaagcgatg gtccatccgg agcgcggctt ctacagcctg ctggcgcagt atccggcgtt
     5401 tacctttagc gcctccgtcg ccaccattac tggcctgctg ttttatgtga cctcggcgga
     5461 ctccggtgcg ctggtgctgg ggaatttcac ctcgcagctt aaagatatca acagcgacgc
     5521 ccccggctgg ctgcgcgtct tctggtcggt ggcgattggc ctgctgacgc tcggcatgct
     5581 gatgaccaac gggatatccg cgctgcaaaa caccacggtg attatggggc tgccgttcag
     5641 ctttgtgatc ttcttcgtga tggcggggtt gtataaatct ctgaaggtag aagattaccg
     5701 ccgtgaaagt gccaaccgcg ataccgcgcc gcgaccgctg gggcttcagg atcgcctgag
     5761 ctggaaaaaa cgtctctcgc gcctgatgaa ttatccgggc acgcgttaca ctaaacagat
     5821 gatggagacg gtctgttacc cggcaatgga agaagtggcg caggagctgc ggttgcgcgg
     5881 cgcgtacgtg gagctaaaaa gcctgccgcc ggaagaggga caacagttgg ggcatctgga
     5941 tttgttggtg catatgggcg aagaacaaaa ctttgtctat cagatttggc cgcagcaata
     6001 ttcggtgccg ggctttacct accgcgcacg tagcggtaaa tcgacctact accggctgga
     6061 aaccttcctg ttagaaggca gccagggcaa cgacctgatg gactacagca aagagcaggt
     6121 gatcaccgat attcttgacc agtacgagcg gcaccttaac tttattcatc tccatcgtga
     6181 agcgccgggc catagcgtga tgttcccgga cgcgtgattg tttttgcata aaaccgcgcc
     6241 atgtctgcat atggcgcggg aatcattgac tcgctgtaaa aaattatgca tatattatat
     6301 atgattgtca tgtcatatcc tgaaattcct cttgtatcac ttcatgactt tatattttat
     6361 taattaacga ttttattttg tattagc
//
LOCUS       DAPPNJ010000224         6324 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000224 DAPPNJ010000000
VERSION     DAPPNJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.226"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            81..425
                     /locus_tag="RPM73_003923"
     CDS             81..425
                     /locus_tag="RPM73_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDX6522224.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            429..764
                     /locus_tag="RPM73_003924"
     CDS             429..764
                     /locus_tag="RPM73_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDX6522225.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(821..3142)
                     /locus_tag="RPM73_003925"
     CDS             complement(821..3142)
                     /locus_tag="RPM73_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX6522226.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGADNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3259..3468)
                     /locus_tag="RPM73_003926"
     CDS             complement(3259..3468)
                     /locus_tag="RPM73_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522227.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(3868..4086)
                     /locus_tag="RPM73_003927"
     CDS             complement(3868..4086)
                     /locus_tag="RPM73_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522228.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(4218..5741)
                     /locus_tag="RPM73_003928"
     CDS             complement(4218..5741)
                     /locus_tag="RPM73_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX6522229.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(6073..6321)
                     /locus_tag="RPM73_003929"
     CDS             complement(6073..6321)
                     /locus_tag="RPM73_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522230.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         1782 a         1445 c         1354 g         1743 t
ORIGIN      
        1 cgagcgttct ttagttaagc agatcttaat tgaagacgaa attattgtgt gtgcaactta
       61 cttaataccg gacatctaag ttgaagaaaa aaatacttgc cttcgggctt atctcagcat
      121 tgttctgctc cacgccagcg atggcggata tgaatcgcac cacgaaaggt gcattgctag
      181 gcgcaggcgt tggtttactt actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg
      241 ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag
      301 gtgcaaaagt aggtgccgcc gttggtgctg tcactggcgt tctgaccggc aatggcctcg
      361 aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga
      421 aataaggaat gatgatgaaa aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa
      481 tgacctgggc gcaggactac aatattaaaa acggtttgcc gtcagaaacc tatattactt
      541 gcgcagaagc caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg
      601 taatgggtaa tgccagcgtt gccagccgtg atttaaaaat tgagcaatct ccggaactga
      661 gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa agacccgcaa atgctgttga
      721 tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg
      781 cgtcgatgaa tacgacgcgg ttttttagct aacggcggga tcagaactta tatttcaggc
      841 ccaccatcac tgcggtgtca ttgtagccat tatcacccag ctgcacgccc acattccccc
      901 agacgctggc cgctggattc aggttgccat ttacgccaag tttaatttcc cccaaattac
      961 tcgccccatc ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat
     1021 ggatccagtt catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct
     1081 cctgagtacg aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat
     1141 tctcaatacg tgagccattg gcttcggtgt gcgcattatg atcgacgccc atccagatag
     1201 cctgtgcctg tggctggaat atccacgtgt atttcgccgc ttccagacca aaggatttat
     1261 tcagggcaaa gccgtaaccc gcttccagcg agacggttgc gcctttcgca ctataggatt
     1321 cataggctaa ttcatcccct ttcaccgagt ttttaaacca gctatattgc gcccaggcgt
     1381 caatgtatgc gcctttttta ctgatgtcat cggcaaacca agtggcatac agccctgcgc
     1441 aatagcctct gacactccct ttcgaacgat aatccgacac gctggaatga gttgagttgt
     1501 agtcgcgggc ataaccagcc atcactccca aacgccaact gtcactgtcg gtaaaaccac
     1561 cggttaacag ctcggcccct aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc
     1621 cgttgctgtc acgccaggca ttatgcccgc caatttgacg tagccaaagt cggcttgaac
     1681 gctcctgttc agttacagga tcgatgtagc gcatttcacc cgcacggtcg ttcagacgca
     1741 taacaaaaag agtgttagcg gctgccaggt tggcgacgta acttccagct tcgaggcgca
     1801 ggacgggggt aggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc
     1861 tcgttgcctg gaagttagtc agataccagt ttttattggt cgcttcggta ttacctcggg
     1921 ttaatgaata ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat tccccatttg
     1981 attccccctc aacgctgata atctctatcc catttaacgt gtaagctccg ctgcctcctg
     2041 cattggtaac tttaactcgg gttgttccag aggtatttcc attaataacg actttatccg
     2101 tagctgaatt atcagcaccc aattcagttc tgagatgcaa tacgccatta ttaccaacgt
     2161 aattttcagt tattgttaat cttgttggtt cgacatcccg tccatctgcc cgggaaatat
     2221 agaccgtact attatcaacc gtgaggttgc tcaccgttga gtcagcttta atatcccagg
     2281 tactattatc tgaaagcgac agataggtat gagtgttaac atctgttgaa atattagctg
     2341 aacccgttat ttttgaatta gttgcatcga cggtcaaatg acctttaata ttattgataa
     2401 aaacatctgc agccgatgat acctcactat ccgtaatggt cacgttactc gtctcatttt
     2461 tatggtagag gatttcattt gctgaccgta gtgagctacc agataaaata tctaccgttg
     2521 cttcctgacc atttatggaa gactccagca caattccaga cccctgatgc gcgactgcag
     2581 atgtattttt cagcgtcacc gtgccgccat tataaatttt agcagtcgcg gcgtcaggac
     2641 ccgttgtata aatttctccc tggttaaatg aaactgtccc ctggcgggca gcgatggcat
     2701 gagcattgtc cccatgcgtg gtgatatcaa catcggttaa tgtaacgctg gcattggtat
     2761 aatcaagcgt gtcaccagcc ttgacgacag tgccattagc gtacgcggca taactttctg
     2821 ttccgtgggt tgtgatctcc ccttttgcca tatccagttt tgctatggca ttattttcat
     2881 tacttcctgt atcggtagta ctttcttcat caagagaaga tacacacgca cgcgcgcttc
     2941 cgggagatgc acacgcaaga acgccgactg cgtgattacc cgcagtaaca ataaatgtat
     3001 catctccgtc agaaaaagag ggggtaaccg aaccgtcaga gttgactctt attatggtgt
     3061 cagtattcag gtttccactc gtttgactta gcaaaccaat tgagtaatcc cccgttatga
     3121 caatcgtgtt tttaccctgc aaaataacag caccgccaaa ttcacttacg attgcgctag
     3181 ctgttaatgc tgtgcttaga ggactattgt cagaaggata taaaccgccc gttgttacaa
     3241 taacgccatt gttaatggtt aacttgctac cctctcctgc aacggatacc cgtaacttcc
     3301 attacccgtt gttataacta gcgcattatc aatatctttt acttttccat cttttattgc
     3361 cacagcatgt ttatattcat cgtgctgaat aaactgttca gcatatgcat tacccaccgc
     3421 cagagcaata agtcccaaac taaacccgga gagttttttc agtttcatag attaaccatc
     3481 cattcattgg attaacaaac attcctggtc aaataaccta tgcgttcaga ttaccaaaac
     3541 ggagggtaaa gacaaaaaca aaagcacaaa aaatttaaat agataaacag cgacaatata
     3601 taaatgataa ataagtcggc attatcattt ttcaccttat tttcatgaca ttgatcactt
     3661 tagggtgacg attttgttta acaaataact cttcgacatc aaaattgtta atagttgcga
     3721 tgaggattat ttcggtgata tataaaaaca accgtgataa caccagggtt gacaactatt
     3781 tgcgcaataa tgcctttcct tcaatgatcc aggcgttcac tgccagcaac ccattcagcc
     3841 gcatactgac agtgagaaac tgaaagatta ttgctgatcc ctgtggttga cgagtctttc
     3901 tcttaatcga ttaaccagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt
     3961 cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca ggagggtgat
     4021 cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt
     4081 gttcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa
     4141 tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg
     4201 gccttactga tcaattttca ctcaaccaca accttcacct tcggtttaga aaggataatt
     4261 ttgaccaggt cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata
     4321 ttattttggt tcagatagtc aagttgttct ttcgtttcga caccttcagc gacgatactg
     4381 atcgaaagtt tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct
     4441 gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatga
     4501 agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt
     4561 tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg ctcacgctcg
     4621 gtgacttcca gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa atttaaacac
     4681 tcgtcgacaa acgtcggcga aataatatgc gaagcgctaa aattaattcc aatatgaaaa
     4741 ccttccggca atttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt
     4801 tgcgtaagcg gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgat
     4861 atatatccac cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct
     4921 tcccgaccat ttaccacagg ttggtaaaaa gggacgatct ccccattatt tattgcccgt
     4981 cgcaggatct cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag gaaggcagcg
     5041 gcgcaggcga ttaacagaat aaatattaaa attccaaagc ctttatcgat aagtctgtag
     5101 aagctaaaga gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc
     5161 ttctcggtat agggataatg tccattaagc gtcatcacat cacccgttgg tccaataatg
     5221 tcatccgcca cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga
     5281 atatgttgat cacttatcgt aaccaaaatg cgcgtatctg caaattggtt ctgatagagc
     5341 aggataggta atctattaac ggtgtcgatt gctggagcca acagtaaatt actgtccggg
     5401 aaaacaggaa tacgagacaa caggacccga ttcccaggca gggatgtaca ccagactatt
     5461 ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc
     5521 tgatattgct cctcgaggtt gcattccttc ccggcaatgt taaccgctgt ttgagtcgct
     5581 tgtgacgctt cttcaagtat atgattgata ttgttggcgg catatctcgc tcccgccagg
     5641 tactctgctt ttgcggaata ccagagctgg atgttcaaaa tagcgacacc ggtaataaac
     5701 aagcatatcg caacgaggat gggtatgagt gtattgcgca tttgcttgtc ccggccaaag
     5761 ggtttaatac atgaaaatgc cattcattcg ctcgcgctgg catctacaga gagcatggta
     5821 gagagcataa gaaatatcca cgccgaagat aaaattttct ccatcttgat ctaacccatt
     5881 aattattgag cataatgtag gcagtacaaa ataagttagg cgggatatca ggcgtcaaga
     5941 atggagagcg agaactctcc attcttgaca cctgatattg cggacataat aagaaagcat
     6001 aacgcctgaa atgctcactt tgcatcagca tggtgataca gctgatgttt attctaaaac
     6061 cttactcaag ttctaagaga gcacggattc cctgtcatta ataatgatat ccgttagtgt
     6121 agtcttatgc atttcaatgt actgcaatat ttgcagatag agatcagaaa gaatcacact
     6181 ggtttcatct tttaaacgca agcgtaaagc ctgatcaata atatgactat caacataccc
     6241 atgcgtcatg aacacctccc tctcgagatt tttaaacaaa gcacattgaa aaataaatct
     6301 ctctgggatt gaattattca tgtc
//
LOCUS       DAPPNJ010000225         6293 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000225 DAPPNJ010000000
VERSION     DAPPNJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.227"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            51..980
                     /gene="pfkB"
                     /locus_tag="RPM73_003930"
     CDS             51..980
                     /gene="pfkB"
                     /locus_tag="RPM73_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDX6522231.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1081..1371
                     /gene="ghoS"
                     /locus_tag="RPM73_003931"
     CDS             1081..1371
                     /gene="ghoS"
                     /locus_tag="RPM73_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDX6522232.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKAPEITITTWEEWNSNRK"
     gene            1477..2337
                     /locus_tag="RPM73_003932"
     CDS             1477..2337
                     /locus_tag="RPM73_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDX6522233.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2378..2914)
                     /locus_tag="RPM73_003933"
     CDS             complement(2378..2914)
                     /locus_tag="RPM73_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDX6522234.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3061..3729
                     /gene="hxpB"
                     /locus_tag="RPM73_003934"
     CDS             3061..3729
                     /gene="hxpB"
                     /locus_tag="RPM73_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDX6522235.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3892..4482
                     /locus_tag="RPM73_003935"
     CDS             3892..4482
                     /locus_tag="RPM73_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX6522236.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4615..6006
                     /gene="tcyP"
                     /locus_tag="RPM73_003936"
     CDS             4615..6006
                     /gene="tcyP"
                     /locus_tag="RPM73_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDX6522237.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6010..>6293)
                     /locus_tag="RPM73_003937"
     CDS             complement(6010..>6293)
                     /locus_tag="RPM73_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522238.1"
                     /translation="NINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1545 a         1549 c         1580 g         1619 t
ORIGIN      
        1 actttcggct gattcggtgc cagactgaaa tcagcctata ggaggaaatg atggtacgta
       61 tctatacgtt gacacttgcg ccctctctcg atagcgcaac aattaccccg caaatttatc
      121 ccgaaggaaa actgcgctgt accgcaccgg tgttcgaacc cgggggcggc ggcatcaacg
      181 tcgcccgcgc cattgcccat cttggaggca gtgccacagc gatcttcccg gcgggtggcg
      241 cgaccggcga acacctggtt tctctgttgg cggatgaaaa tgtccccgtc gctactgtag
      301 aagccaaaga ctggacccgg cagaatttac acgtacatgt ggaagcaagc ggtgagcagt
      361 atcgttttgt tatgccaggc gcggcattaa atgaagatga gtttcgccag cttgaagagc
      421 aagttctgga aattgaatcc ggggccatcc tggtcataag cggaagcctg ccgccaggtg
      481 tgaagctgga aaaattaacc caactgattt ccgctgcgca aaaccaaggg atccgctgca
      541 tcatcgacag ttctggcgaa gcgttaagtg cagcactggc gattggtaac atcgagttgg
      601 ttaagcctaa ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc cagccagacg
      661 atgtccgcaa agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg gttgtcgttt
      721 cccttggtcc acaaggagcg ctgggtgttg atagtgaaaa ctgcattcag gtggtgccac
      781 caccggtgaa aagccagagt accgttggcg ctggtgacag catggtcggc gcgatgacac
      841 tgaaactggc agaaaatgcc tctcttgaag agatggtccg ttttggcgta gctgcgggga
      901 gtgcagccac actcaatcag ggaacacgtc tgtgctccca tgacgatacg caaaaaattt
      961 acgcttacct ttcccgctaa caaaaacatt cccccagcat tgggggaatc accaccaacc
     1021 tgtcggcaac gcgtttctcc gactatgctc aaaagtcatg tgataacaaa ggggtgaact
     1081 atggccagtg gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga ggatacattg
     1141 actgaaatta acgagttgaa taattacctg actcgcgacg gttttttgct caccatgacg
     1201 gatgatgagg gaaatatcca tgagctgggg actaacactt ttggacttat cagtacccaa
     1261 agtgaagaag aaattagaga actggtttcg gggcttaccc aaagtgcaac cggcaaagct
     1321 cctgaaatca ccatcacgac ctgggaggaa tggaatagca acagaaaata aatggttttt
     1381 gggcaataat cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat tcttgcgcta
     1441 accttatgat ctggcagaca acatgggaga gacatcatgt ggcaggcaat cagtcgtctt
     1501 ttgagcgagc agttaggtga aggcgaaatc gaactgcgta atgaactgcc tggcggagaa
     1561 gtccatgccg catggcattt gcgctatgca ggacatgact ttttcgtcaa atgtgatgaa
     1621 agggaactgc ttcccggttt taccgccgaa gccgaccaac tggagttact gtcgcgtagt
     1681 aaaaccgtca ccgtgcctaa ggtttgggca gtaggcgctg accgtgacta cagttttctg
     1741 gtgatggatt atctcccacc tcgtccgctg gatgcgcata gcgcatttat tcttggtcag
     1801 caaattgcgc gtttacatca atggagtgac caaccacaat ttggcctcga ttttgataac
     1861 tcgctctcca caactccaca gcccaacacc tggcaacgtc gctggtcaac gttttttgct
     1921 gaacaacgga ttggctggca gttggaactg gcagcagaga aagggatcgc tttcggcaat
     1981 atcgacgcca tcgtcgagca tattcagcag cgtctggcct cgcatcaacc gcagccttct
     2041 ctgttgcacg gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg cccgtacatt
     2101 ttcgaccctg cctgctactg gggtgaccga gagtgcgacc tggcgatgtt accgctgcat
     2161 actgaacagc cgccacaaat ctatgacggc tatcagtcag tatccccgct acctgccgat
     2221 ttccttgaac gtcaaccggt ttaccaactc tacacgctgt taaatcgtgc aagattattt
     2281 ggcggtcagc atttggttat tgctcagcag tcattggata gattattagc agcatgatat
     2341 gggttgagga taatggccgc tccgtgcggc cttttgatta aataaaccca agcagagaaa
     2401 agaacacata acctgcagca atacaaataa ccggcaggat atatagtgag aaaaactgca
     2461 ggaaaatagt atggtgcgga acaacaatac gggactcaat ttgttcacgc gttagcccct
     2521 cttccccttt ggctttttcc agaatgagtt gatcttcaac accttcccgc aagaagcgcg
     2581 cctggcgact catccgggca ccggaatcct gcaacgccag tccgacaaaa atcaggatga
     2641 aaatcaccca gaacataacg ttcacgccac cattaaaatt aggggtcggg gagttatacc
     2701 agaacagatt caaaaaaggc gtattcgcct gcatcatatc gatcatgaca tgagtaaagt
     2761 cgagcatgac cgcattaata ccttcctggt tttcctgccg ggtattcata aatttcagca
     2821 aagaaatcaa tgtagagatc aatgcaggta taaaaatcac ccaacccaaa atccttttca
     2881 aaacagcaat gcgtccagct tgttgatacg tcatgagttc tccttgatta agacgcgtcg
     2941 tttcacttag tttacctgta gatatctgtt ttcgcccatt ctttaaaggc gatatgatag
     3001 gcgcttaatc ataagcacgg cttaatacct tacacataat gctctaaagg agaggttgta
     3061 atgtcaaccc cgcgtcagat tcttgctgca atttttgata tggatggatt acttatcgac
     3121 tcagaacctt tatgggatcg agccgaactg gatgtgatgg caagcctggg ggtggatatc
     3181 tcccgtcgta acgagctgcc ggacacctta ggtttacgca tcgatatggt ggtcgatctt
     3241 tggtacgccc ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga acgggttatt
     3301 gcccgtgcca tttcactggt tgaagagaca cgtccattat taccaggcgt gcgcgaagct
     3361 gttgcgttat gcaaagaaca aggtttattg gtgggactgg cctccgcgtc accactacat
     3421 atgctggaaa aagtgttgac catgtttgac ttacgcgaca gtttcgatgc cctcgcctcg
     3481 gctgaaaaac tgccttacag caagccgcat ccgcaagtat atctcgactg cgcagcaaaa
     3541 ctgggcgttg acccactgac ctgcgtggcg ctggaagact cggtaaatgg catgatcgct
     3601 tctaaagcag cccgcatgcg ttccatcgtc gttcctgcgc cagaagcgca aaatgatcca
     3661 cgttttgtat tagcaaacgt caaactctca tcgctgacag aactcaccgc aaaagacctt
     3721 ctcggttaat gaccaggggc agtgatcacg ctgcccctgg ttctttatct gaatttgcat
     3781 tcaactgacg gattaatcgt caatttaaga gaaagagtta caccgtcacc acttccgtgc
     3841 actgtataaa aatcctatac tgtacgtatc gacagtttag tgagttttat catgacggcg
     3901 gaaggtcacc ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg
     3961 cccgtgctgg cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg
     4021 ttaccggaca tcgatcaccc aaagtcgttt cttgggcagc gattaaaatg gatatcaaaa
     4081 ccgatcgccc gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg
     4141 ctgctggcaa ccttttacct taaggttccg gaaggctggt tcattccggc tgatgcgcta
     4201 caaggaatgg tgctgggtta tttgagccac atacttgccg atatgctgac acccgccggt
     4261 gttcccctgc tctggccatg ccgctggcgt ttccgcttgc ctatcctggt tccccaaaag
     4321 ggcaaccaac tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgccc
     4381 cattcattac ccgagaacag cgctgttcgt tggtcatcgc aaatgatcaa taccttgcag
     4441 atccagtttc atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcacctt
     4501 tctggaataa gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg
     4561 ccaacactat gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac
     4621 tttccattaa ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc
     4681 cgccataaac agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtggtt
     4741 tttggccttg ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta
     4801 cagtggttta acatcgttgg taacggctat gttcaactgc tgcaaatgat cgttatgccg
     4861 ttagtcttcg cctctattct gagcgcggtt gcccgtctgc ataacgcatc tcagttaggc
     4921 aaaatcagtt ttctgaccat cggtacgctt ttgtttacca cgctgattgc ggcgctggtc
     4981 ggtgtgctgg tcaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca
     5041 gaaactgcac gtctgaacgc cattgaaagt aactatgttg gtaaagtctc tgatctgagc
     5101 gttccgcagc tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct taccggagcc
     5161 aatccgacgt caattatcag cgtggtaatt tttgccacat tcctcggcgt agctgcattg
     5221 aaactgctga aggatgatgc gccgaaaggt gaacgcgtct tagccgctat cgatacccta
     5281 caaagctggg tgatgaaact ggttcgcctg gtcatgcagt tgacccctta cggcgttctg
     5341 gctctaatga ccaaagtggt tgcaggttct aacttgcaag acatcatcaa actgggaagt
     5401 ttcgttgtcg cgtcctacct cggtctgctg attatgtttg cagtgcatgg cattctgctg
     5461 ggcattaatg gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt gctgacgttt
     5521 gcctttacca gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt
     5581 cgtctgggcg ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc aaccattggt
     5641 cagaacggct gcgccggttt gtatccggca atgctggcgg tgatggttgc gcctacggtt
     5701 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     5761 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     5821 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     5881 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     5941 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     6001 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     6061 atgttccatc gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat
     6121 gtccaactta tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac
     6181 ctcatacccg agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac
     6241 accacttccc attcccggaa gatttagtgg attaaaaaga agattgatat taa
//
LOCUS       DAPPNJ010000226         6292 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000226 DAPPNJ010000000
VERSION     DAPPNJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.228"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            868..1434
                     /locus_tag="RPM73_003938"
     CDS             868..1434
                     /locus_tag="RPM73_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDX6522239.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFAERIAECWQAITYNCGSAHRCTYQPHYAYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1681..2256
                     /locus_tag="RPM73_003939"
     CDS             1681..2256
                     /locus_tag="RPM73_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522240.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPWEHDNAFNSRQTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEALFKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
     gene            2325..2561
                     /locus_tag="RPM73_003940"
     CDS             2325..2561
                     /locus_tag="RPM73_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX6522241.1"
                     /translation="MLTSMTGHDCVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2558..2716
                     /locus_tag="RPM73_003941"
     CDS             2558..2716
                     /locus_tag="RPM73_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522242.1"
                     /translation="MKRVAMSALVLRKSALSLVLATLPFIFLLTLSGQPADCSSFLTD
                     SSFHSHCL"
     gene            2804..3316
                     /locus_tag="RPM73_003942"
     CDS             2804..3316
                     /locus_tag="RPM73_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522243.1"
                     /translation="MQITEALISEPGDIRRFIQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEQCSQVVDLLQQTWRGMDTGGQCRVERCFRVTRPDTSEQYVALKTAVQSL
                     RPLVIATIIR"
     gene            3405..4028
                     /locus_tag="RPM73_003943"
     CDS             3405..4028
                     /locus_tag="RPM73_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX6522244.1"
                     /translation="MRARIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDIYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTHKIPAYHL"
     gene            complement(4207..5055)
                     /locus_tag="RPM73_003944"
                     /pseudo
     CDS             complement(4207..5055)
                     /locus_tag="RPM73_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5395..6132
                     /locus_tag="RPM73_003945"
     CDS             5395..6132
                     /locus_tag="RPM73_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDX6522245.1"
                     /translation="MKKVIAVSALAMAGMFSAQTLADESKTGFYVTGKAGASVMSLSE
                     QRFVDGEGAWADKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
BASE COUNT         1556 a         1481 c         1515 g         1740 t
ORIGIN      
        1 actctcaatc tttacatatt ccattagtat tactcttgtt atcgtattca ctccatcccc
       61 caatcataat tactgtaact catatgatat tcagataagc tattctctac tacgctattg
      121 ctaatatcca tctgttatca gtcttcactg aaacagcaat tgatcttaat cttatttatc
      181 atgaactgta tttacttaac gataattgtt tatctgaagt gttttaacta ttctggtgaa
      241 catcaattct tttgtcatca ctgattaact gaatgtttac tctttgatac agtatccatg
      301 attccgtcat gtttaacaga gctggataaa caacagaatt aacagagtga atttctgatt
      361 atatttgttg ccagttgtat tgtttaaggt actgggtgaa aattattcat ccatggtatg
      421 ttgtcttatg ctatcgtgtg tcgttaacgt tcatatcctg gagaacagat tgaataagcg
      481 cgtgtgagtt tattgtggtg accttataaa cgttttttca gactcactga gagcaaattt
      541 acaaatcatg agcttatagt tgtaatatgt ttcctaaccg cctggcagcg ggattacagt
      601 tcgtattttt ctgtatttca ctgtgttgta ttgaatctgt attttcttcg cacctcgttt
      661 atctcatctc tatagacaat gccatcagat aactcatacc cttcagcaca acgctgtgtg
      721 tcgttaataa tccgtcgtat ccattctgtt acagcatgcc tgataaatac catctgtaag
      781 ttattaccgt tttagatctg attatgagcg aaagcattaa ttcgctcaca gagcttaaaa
      841 catcattaac tttcaggagt catcaacatg cctaaatctt acacaccaaa ctggtttttt
      901 accgctttac ttgacaatca catcaatcaa atgatggcac gctattcctg cctgcgggcc
      961 ttacgcatgg atttcttcta caggaaagat acgcctgatt tcttacaacc tgatcatcgc
     1021 tggcttgaat tgcagttgcg tatgctgctg gagcaggtgg aacaatttga aaatatcgtt
     1081 ggtttcttct gggtgattga atggacggct gatcatggtt ttcatgcgca tgtcgttttc
     1141 tggatcgatc gtcagagggt taaaaaaata tatccctttg cggagcggat agcggaatgc
     1201 tggcaggcaa ttacgtataa ctgtggttcg gcgcaccgct gtacatatca gccgcattat
     1261 gcatacaaca tcaacattcc tgtgcgccac aacgatcctg aaagcatcga taatattcgc
     1321 ggtgccctgc attatctggc gaaagaagaa caaaaagacg ggctgtgtgc ctacggctgc
     1381 aatgaagttc ctgaacgtcc tgctgcaggg cgtcctcgta agcctcactt ctgaagctta
     1441 aggcctgagc ctccgctcct ggaaacactc cgtcggtaaa aacttaccgc attgattaat
     1501 gatgtgaact gaagtcaacg gagatcattc atcctgaacc tgaatccggt gttctgtccc
     1561 ttatcttccc gttctgcttc agttcttcac ttattccatc aatctcatcc gcaagccata
     1621 acacgtcagc tcattcacgg gcaggacgca ttgtgggctg cgcataacgg aacatatctt
     1681 atgaatgcta ttccttattt cgactatagc ctggcaccct tctggccatc ttatcagaac
     1741 aaagtcatcg gcgtccttga gcgtgcgctg cgtgagcagt ccggctcacg gatacggcgg
     1801 atcctgcttc gtctgccgtg ggaacatgac aacgccttca acagcagaca gacctggttt
     1861 ggtatggatt ttatcgaaac cgtcagtgcg ctgatgaatg cgaaacccgg acgcgacctt
     1921 tgctggctcc tgacccgtca tccggaaaag ccggaatacc acgtggtgct gtgcgtcaga
     1981 caggagtatt tcgacggccc cgaactggac cggctgattc tggatgcctg gagcaacgta
     2041 ctgggcttcg cgtcaccggg ggaagcggca ccgtaccaga aacagataac ccgggatgtg
     2101 gtgctggaca gtcgttcacc ggactgcgaa gccctgttta aggaccttat ctgggcgttc
     2161 agtgatttcg cccgcgatcg ccgtggagtg tgcgatccgg aagcccgttg cctcgccggt
     2221 aatcccggct atcccggatc tgcaggaccg ttctgagaat acggctcagc acctttcccc
     2281 gcattcgcac aaccttcata aatctcacag aggatattct gaccatgttg acctcaatga
     2341 caggccacga ctgcgtgttg ctgcgtgccg acgatcccct gatcgacatg aactacatca
     2401 ccagtttcac cggtatgaca gataaatggt tttacaagct gatcagtgaa ggccatttcc
     2461 cgaaacccat caagctgggc cgcagcagcc gctggtacaa aagtgaagtg gagcagtgga
     2521 tgcagcagcg aattgaggaa tcacgaggag cagcagcatg aaacgtgttg caatgtcggc
     2581 gctggtattg cggaaatcag ccctgtccct ggtgctggcg acattaccgt tcatcttcct
     2641 gctgaccctc tccggtcagc cagcagattg tagctctttc ctgactgatt cgtcattcca
     2701 ttcacattgt ttataactgg cattacaccg gtgttgatgc gcaccttccc gtgtgtctgc
     2761 accggctgaa caaaattcaa cagggtttga aaaggaacat ttcgtgcaaa taaccgaagc
     2821 attaatttca gagccgggag acatccggcg ttttattcag caggcggtgg accactggcc
     2881 gcatttgctg gcattttact tcacactcca ttcggcagaa ggaaatatca acgggcaaca
     2941 gattcatgca ttctgtactt ccttttaccg acaggtgcac gaacgtatta ctgagcgtaa
     3001 tcacacggcc agtccatcct cgccggtggt attacgctgg ttgcgggaac aacatggagg
     3061 agcaacaata cgatgcctgt tgctgttcag ccaggagctt ttctgccatc cgcgagccag
     3121 tgtgacagtt gatgaacaat gttcgcaagt ggtggattta ctgcagcaga cctggcgggg
     3181 gatggatacg ggcggacaat gtcgggtgga aaggtgtttt cgggttaccc ggcctgatac
     3241 atcagagcaa tacgttgcgt taaaaacggc tgttcagtct ctgaggccac tggttattgc
     3301 aacaatcatc cggtgatgac cgggagaaat atcgactctt ccgttaattt ttacagactg
     3361 ataaagtttt gtctgctgaa tctttatacg gaattctttt cagtatgcgt gcacgaattg
     3421 attatctggc agataaatac tgttttacag aacggaacga atcgccccgt cttcgccggc
     3481 agtggcagga tgttctggag gagtgtcggc agacagaggc cgggccagaa gaacgactgc
     3541 gtattgcatt gttgaatgtg gattacgtca ccagttttga gctgcctttt cgcttgctgc
     3601 ttacccggac accacaactg attgccgcgc tgcgtgaaga atggggcatc agccagaaaa
     3661 atgtggtgtt caacgataaa cggtttggct gcgtgtacag cctgaaggcc agtctttctg
     3721 gtgtgccgga tatataccgg tatcatctgt ctcaccgtat tcgtcgcgtg gttgggaatg
     3781 aaagcacatc atcgccatat cagcaggttg ccagggaagt gaaagcgccc cgtgaacggc
     3841 tgaagtatgc gctggaggca ggtttactgg tgactgcact ggacggcctg ttctggtttg
     3901 gcagtcagcg tattgcggct gatgtcctga ggctgagaaa ggccggaatg ccggtggtga
     3961 ccacgaccgt ggaggtgcac gataacctga cgggaacaac ccacaaaata ccggcatacc
     4021 atctctgata caaccagcag gggggaatat tgctctggca gcggcagtac gacattcttt
     4081 atactttatc cccatgcctg aaaacatgtg ctaaggaaac gggaatggca aggtccgatt
     4141 acgatattat caatctgtct ctggaacatg aactgaatga gtggctggca gagaaaggtg
     4201 tagtggtcaa gtaatattgg ccacggtttt acagtaaaaa cggtatctgt tctctgactc
     4261 ttccggcgtc aacccaccgt tgtaatggtg aggcctgacg ctattgtaat aattcaggat
     4321 ataaccgcta atttgttgcc gggcctcgtc cttgcctacg taaccatccg tcggcaccca
     4381 ttctgttttc agactgcgga agaagcgttc cataggactg ttatcccagc agtttccccg
     4441 tctgctgaca ctttgcttta tcctgtaacg ccagagaagc tgttgatatt tcagtcctgt
     4501 atactgactt ccctggtcgc tatggaacat gacgtcccgc ggctgaccac gcacctcata
     4561 cgccatccgc aaggcactgc ttatcagtgc agtatcggca ttcgctgaca ggctccagcc
     4621 gataaccctg cgggcaaaaa gatccatgac gaccgccaga tagcaccagc gatttcctgc
     4681 ccagatatac gtaatatctc cgcaccatac cctgtctggc tccggtacag cgaactgacg
     4741 ctcgagcaga ttcggcaaac acgtatgctc ctgacaggca tttttgtatt gatgttttcc
     4801 gggctgacaa ctgctcaggt tcaggtattt catcagccgt ccggcacgat aacggctcat
     4861 cgggacgccg ttttgggtca gcatttcagc cagcgtgcgc gcgcctgcag agccccggct
     4921 ctggttccat gcccggcgta tttcgctgca caacctgacg cgagccgaat taaccgtatc
     4981 gcgtcgtttt cgccagtacc ggtaactgca aaatggcatc tgaatcgatc gcagtttggc
     5041 attcgaatcg atcatgattt ggcggcgttt ttcaaggtag ttgtcgcctt ttgcaataaa
     5101 cgtgttttct ctggtggggt gattacccag tcttttttac tgtgtctgac attttgtact
     5161 gtagtgcagt tgtaatgcaa acctcccagg cttctgggac acagcatttc agtccgggcc
     5221 ccatcattac cattttgttg attcgaaacg aaaaccctca ccaatatcga tcgattttac
     5281 agatcgatat gatcacatcg atcggttgta actattttac cctgtctttt gcatggtttt
     5341 aaaataggta accctctttg tcgggtaggg cacgaaccaa cggagatgat tgaaatgaaa
     5401 aaggttattg cggtttcagc gcttgcaatg gcaggcatgt tttcggccca gactctggct
     5461 gatgagagca aaacaggctt ttatgtgacc ggtaaagcag gtgcttctgt tatgtcactt
     5521 tcagagcaac gtttcgttga tggtgaaggg gcatgggcgg ataaatataa aggcggtgac
     5581 aaaagtgata cggtttttgg cgcaggcctt gcggtgggat acgattttta tcagcactac
     5641 aatgtgccgg tgcgtactga agtggagttt tatggccgcg gtaacgctga atcaaaatat
     5701 cgtctgagtt attgggaaag tgtcggtggt gccgagtttg atgatgcaca gaacaagctc
     5761 agtgtaaaca cgctgatgct gaatgcgtat tatgatttca gaaacagcag tgcattcacg
     5821 ccatggatat ctgctggtct gggttatgct cgggttcatc ataaaacttc gtacatatat
     5881 accgataaca gtccggcagg cagtgaagta tattcagctt cagcgtcaaa atacgaaaat
     5941 aacctggcat ggagccttgg tgtcggtgtt aaatacgatg tgacgcagga tttcagcctt
     6001 gacctcagtt accggtatct ggatgcaggg gattccactc tgacttataa agatgaggat
     6061 ggcgctaaat ataaatcctc tgttgatgtc agaagtaatg agtttatgtt aggtgcaaca
     6121 tataacttct gatggccatt tctgcctgcg ctttggagca cagtaaagtg caggtatgac
     6181 aaatcaataa cagatatcct gatgatattc tccctctggc aactaataat ttcagatgaa
     6241 tgaaatataa atagagcaaa aatattactc tcgttgattt tttacagggg gc
//
LOCUS       DAPPNJ010000227         6221 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000227 DAPPNJ010000000
VERSION     DAPPNJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.229"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            171..2414
                     /gene="pdeF"
                     /locus_tag="RPM73_003946"
     CDS             171..2414
                     /gene="pdeF"
                     /locus_tag="RPM73_003946"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDX6522246.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2453..3994)
                     /gene="ppx"
                     /locus_tag="RPM73_003947"
     CDS             complement(2453..3994)
                     /gene="ppx"
                     /locus_tag="RPM73_003947"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDX6522247.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3999..6065)
                     /gene="ppk1"
                     /locus_tag="RPM73_003948"
     CDS             complement(3999..6065)
                     /gene="ppk1"
                     /locus_tag="RPM73_003948"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDX6522248.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
BASE COUNT         1530 a         1493 c         1513 g         1685 t
ORIGIN      
        1 taataattca ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga
       61 cacttagaat caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg
      121 cctgtctgaa tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga
      181 atgcaactta tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt
      241 taatcttgcc cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaggttt
      301 ttctctttta tctgcctttg gcactgatga tcagcatgat gatgtttttc agctgggcgg
      361 cattgccagg gatcacctta gggatttttg tccgcaaata tgcagagctg ggtttttacg
      421 aaacgctctc attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca
      481 gggtctttac tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc
      541 gtatattctg gcagattgtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg
      601 catttgtagg attactggcg agcagagaaa atctggtcgg tgtcatgcct tttaacctcg
      661 ggactttaat caattatcag gccttactgg tgggtaatct gatcggtgtc ccgctgtgct
      721 acttcatcat tcgggtagtg cgaaatccat tttatttacg tagctattat tcgcaattaa
      781 aacagcaggt tgatgccaaa gtcaccaaaa aagagttcgc gctctggcta ctggcattag
      841 gtgctttatt gttgctgtta tgcatgccgt taaatgaaaa aagcacaatt tttagcacca
      901 attatacctt gtcattattg ctgcccctga tgatgtgggg agcgatgcgc tatggttata
      961 agctgatttc gctgctctgg gcggtcgtgt tgatgatcag catccacagc tatcaaaatt
     1021 acattcccat ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg
     1081 tattctcttt tattgtcaat tatatggctg tactggcaac tcgtcagcga gcggtagtca
     1141 gacgcattca gcggcttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc
     1201 tgaatcgcgc gttgcgtgat gccccctggt ctgcgctttg ttatttacgc atccctggca
     1261 tggaaatgct ggttaagaac tatggcatca tgctgcggat tcaatacaag caaaaacttt
     1321 ctcactggct gtcacccttg ctggaaccgg gtgaagatgt ttatcagctt tcgggtaacg
     1381 atctcgcgct gcggctgaat acagaatcgc accaggagcg cattaccgca ctggatagcc
     1441 atctcaagca atttcgtttc ttttgggatg gcatgccgat gcaaccgcag attggcgtca
     1501 gttactgcta tgtgcgctcg ccagtgaatc atatctacct gctgctggga gagctaaata
     1561 cggtcgccga actttccatc gtgaccaacg ccccggaaaa tatgcagcgt cgcggggcaa
     1621 tgtatttgca acgcgaattg aaagataaag tcgcgatgat gaatcgacta cagcaggcgc
     1681 tggaacacaa ccattttttc ctgatggccc agccgattac cggtatgcgt ggtgatgttt
     1741 accatgaaat tcttctgcgc atgaaaggtg agaatgatga actgatcggc cccgatagct
     1801 tcttgccggt cgcgcacgaa tttggtttat cgtcgagtat cgacatgtgg gtcattgagc
     1861 atacgctgca atttatggct gaaaacagag cgaagatgcc cgctcaccgt tttgctatta
     1921 atctgtctcc aacctcggta tgtcaggctc gttttcctgt tgaagtcagt cagttgctgg
     1981 ctaaatatca gattgaagcg tggcaactta tttttgaagt caccgaaagt aatgctctga
     2041 ccaatgttaa gcaggcgcaa atcaccttgc agcatcttca ggaattaggc tgccagattg
     2101 cgattgatga tttcggcacc ggctacgcca gctatgcacg gcttaaaaat gtgaatgccg
     2161 atctgcttaa aattgacggc agttttatcc gcaatattgt gtcaaatagt ctggattatc
     2221 agatagtggc atcgatttgc cacctggcgc gaatgaagaa aatgcgggta gtggcagagt
     2281 acgttgaaaa cgaagagatc cgcgaggcgg tgctctcttt ggggatcgat tatatgcagg
     2341 gttatcttat tggtaagccg cagccgttaa ttgatacgtt gaatgaaatc gaacccattc
     2401 gcgaaagtgc ctgaataatg cgggccgaca tttcttgtcg gcccgcaaag cattaagcgg
     2461 cgatttcagg tgtactttct tcttcaattt tcaaccgcca gccagccacg ccttcccagt
     2521 attcttgctc cttttccaga tcaagcagta ccagcgcatt ctgactaaac cagtcatgcg
     2581 ggaaacgcag tgtccagtga ctgtcatcag taatcagtgt caatgttggc ggtgtggtgg
     2641 ttgcctgacg ttgattgttt agtaatacgc caaggcgcaa tagctgtatc agtggcagga
     2701 actgtttctt cttaaacaag gtaaagcgcg gcagatcgtc gagcttaatc gctttacggt
     2761 gatagcgcac cagtgtcgcc atcatcagct gctgttcctg attaaaaccc ggcaagtcac
     2821 tgttttgcag aatataagcg gagtggcgat gcaaaccgct gtggttgata ttcaacccga
     2881 cctcatgcag catggcggcc catcgcagta gcgcctccag ttgcggatgc gccagcttcg
     2941 gttgctgttc ccgccactgt tcgtacattt gcatagtggt atccagcacc cgtcgggcct
     3001 gctcgctgtc gatgtgatac tggttggcga ggctgctggc ggtgcgacta cgcacatcct
     3061 gatgacggaa acgtccttcc atttcataca gtacgccttc gcgtaaagcc ccgtcagaaa
     3121 ggcgcagttc acggatggct aaagcatcaa acacaccgca taaaatcgcc agtccaggaa
     3181 cgaagactgt tttccgctct tcggaaagac ccggtaaact cagcgatgcg aaattacggt
     3241 gacgtaaaac ttcttttacc agtttttcca gacgttccgg ggtaattatc ccgtctttct
     3301 cgcccatttc cattaacact tcatgggcgg cttttatggt accggaagcg cccattgcca
     3361 cgttccagcc ctgaatacgg aattgccagg ttaaagtttc cagtttttgt gctgccgcca
     3421 tacgagcgcg ctgaaaattc tctttattga tgaccccgcc agggaagtaa agctgggcaa
     3481 agctgacaca acccatccgg cggctttcaa cgagaatagg ttcgaaattt tcaccaatca
     3541 ccagttccgt agatccgccg ccaatatcaa taaccagttt gcgacctttc tccggttggg
     3601 tatgttccac gcccataaaa atcagacggg cttcttcatt accggaaata atttcaatcg
     3661 ggtagggaat gaccttttcc gcgcgtttca gaaagtcagt ggcgttcagc gcctgacgca
     3721 gcgtatgggt gccgactata cagacgctgg caggagaaaa cccttgtagc cgttcggcaa
     3781 acagcgacag acagttcaaa ccgcgcgtca ttgcctcttc actcaacata ttatctggcc
     3841 ccaggccgtc cgccagatgc acccgctgtt tcaggcggcc aataatctgc atggcaccat
     3901 ctaccacacg ggctatgacc atgtgaaaac tgtttgaacc aagatcgacc gcagcaaact
     3961 cctgcggacg aggggattta tcgtgtattg gcatagggtt attcaggttg ttcgagtgat
     4021 ttgatgtagt cataaatcgc caactgcgcc cgtactttgc ggcgattgcc gcggggaacg
     4081 tagcgattac tgagttcttt atcgatataa cgagctttga ccgtatcgct gaacaatatg
     4141 tcgatgatgt ccagcacccg ctgcttcagg cgcggatcga gcagcggcgt cgccacttca
     4201 atacgataat caatattgcg cgtcatccag tcggcggaag aaaggtagac ctttttatcg
     4261 ccgccatttt caaaaatata aacccggtca tgttcaaggt aacggtcaac aatactgatg
     4321 gctcgaatgt tgtcgctaat gccttccaga ttggggatca gcgaacacat tccgcgaacc
     4381 agcagattaa ccggtacgcc ggagctggag gccgcataca gacgatcaac caggccttta
     4441 tcgacaaggt tatttagctt cagggtgata ccgctgggca gcccttgctg cgcgttggcg
     4501 atttcgcggt ctaccatttc atataacaga cggcgagagt tttgcggtga caccattaaa
     4561 taatcaaatg tcaccgggcg gtatgggttt tcaataaagt taaatacccg ccgtacttcg
     4621 ttggtgatac gcgcatcggc ggtcagcaat gaatagtcag tataaagacg cgcggttttt
     4681 tcgttaaagt tcccggtccc gatgtgtgcg taacgcacca cttcaccgtt ttcttttcgt
     4741 gaaatcagga acagtttggc gtgaattttc agccccggcg cagagaagat aacgtgcacg
     4801 cctgcttcgg tcaggcgctt cgcccagtga atgttagctt cttcgtcgaa acgcgcctgt
     4861 aactcaacca ccacggtgac tttcttaccg ttatgtgcgg cgtggatcat cgagtcgatg
     4921 atgcgtgaat ctttcgccac gcggtaaata ttaattttaa tcgccagtac gctcgggtcg
     4981 aacgaagcct gacgcagcag ttccagcaca tgctcaaagg tgtgataagg ataatagagc
     5041 aacacatcgc gttcgcgaat ggcatcaaaa ccattgcgga actgggcttt atcaaaccaa
     5101 atatggcgta aacgcggcag tggtttgttc accagattgg ctttgccgac attggggaaa
     5161 ttaataaagt ctttaaaatt atgataacga ccgccgggga cgatggagtc gtagcgggaa
     5221 atagtcagtt tttcgcgtaa cacttcaacc agcgcattgg gcatatcgcg ctgataaaca
     5281 aaacgcaccg gctcagcagt taaacgctgc ttgagactgg aagacatcaa ctccatcagg
     5341 ctggcttcca tctcatgcac taaatcgtat tcggcatcgc gggtcatctt cattgaatag
     5401 gcattcagcg cgtcataatc aaagaagcct ttgaaaatat catcaaggca gtaacgcaga
     5461 atgttatcca gaagaatcat cggcttgcgt cgacgcggcg cttctggcgg taaattcaca
     5521 aagcgcggca ctttatctga tgggatctcc agcagcgcgt aacggatggt atcgccacgg
     5581 ataatttcca ccgccagata ggtgtaatca tctttcagga actgcactaa gtcagtgtca
     5641 ggattgatta aaatcggcgt aatgtgctga cgcagatact gcttaaaata atgacgcagc
     5701 cagttttgtt gattgacgga gagctggcgt tcattaatca ggaagatctg gttacgcgcc
     5761 atctccagca gcagctcgtt gtagaggccg tcgaattcct gatcggcttt cagcacccgg
     5821 gactgaattt tgcccagtaa atggcgggaa tgagagttgg agccttgttc ttcgctaata
     5881 atgatgcgtc gcttcagttc agcgaagcgg actttataga actcatcaag gttattggaa
     5941 tagatcccca ggaaacgcat cctttcaatc agcgggttag atttgtccgc cgcttcctga
     6001 agcacgcgtt cattgaacga taaccaactg agctcttttt cgatgtatag cttttcctga
     6061 cccattacca cttttactcc gttttattca cgggacactg cctggatatt atggcgagca
     6121 atattggcag atgtccaact atgccagaaa agggggaaaa aaactgctgg agcttgcgat
     6181 ttacaggtga attgaaaaac gccagcggca gagctggcgc t
//
LOCUS       DAPPNJ010000228         6179 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000228 DAPPNJ010000000
VERSION     DAPPNJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.230"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1441
                     /locus_tag="RPM73_003949"
     CDS             <1..1441
                     /locus_tag="RPM73_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDX6522249.1"
                     /translation="AKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSD
                     WGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVL
                     AAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALA
                     RRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRI
                     VPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLP
                     YEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAPTLKVQGV
                     DLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGV
                     AGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDN
                     SLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1477..1680)
                     /gene="ydfZ"
                     /locus_tag="RPM73_003950"
     CDS             complement(1477..1680)
                     /gene="ydfZ"
                     /locus_tag="RPM73_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDX6522250.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1858..2544)
                     /gene="ydfH"
                     /locus_tag="RPM73_003951"
     CDS             complement(1858..2544)
                     /gene="ydfH"
                     /locus_tag="RPM73_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDX6522251.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2633..3379)
                     /gene="ydfG"
                     /locus_tag="RPM73_003952"
     CDS             complement(2633..3379)
                     /gene="ydfG"
                     /locus_tag="RPM73_003952"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDX6522252.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3516..5561
                     /gene="dcp"
                     /locus_tag="RPM73_003953"
     CDS             3516..5561
                     /gene="dcp"
                     /locus_tag="RPM73_003953"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDX6522253.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5606..6124)
                     /locus_tag="RPM73_003954"
     CDS             complement(5606..6124)
                     /locus_tag="RPM73_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDX6522254.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1527 a         1488 c         1584 g         1580 t
ORIGIN      
        1 agcaaaagcg acactccctg tttatgatcg taataacctg gccccaagaa ttgttcattt
       61 aggctttggt gcatttcacc gtgcgcatca gggcgtgtat gccgatattc ttgctacgga
      121 acatttcagt gactggggat attatgaggt caacttaatc ggcggcgaac agcaaattgc
      181 cgatttacaa cagcaagata atctttatac cgttgcggaa atgtcggccg acgcgtggac
      241 ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta caaatagatg gcttagaaac
      301 cgtgctggct gcgatgtgtg aaccgcaaat tgcgattgtc tctctgacaa tcaccgaaaa
      361 agggtatttc cactctccgg cgaccggaca gttaatgctc gatcacccga tggtcgctgc
      421 cgacgtgcaa aatccccacc agccgaaaac agcaacaggg gtgattgttg aggcgctggc
      481 tcgccgtaaa gcggcaggac ttcccgcatt taccgttatg tcatgtgaca acatgccaga
      541 aaacggtcat gttatgcgtg acgttgtcac ttcctacgcg caagccgttg atgtaaaact
      601 ggcacaatgg atcgaagata acgtgacttt cccatcaaca atggtggacc gtattgtgcc
      661 cgcagtgaca gaggatactc ttgcgaaaat cgaacaactt accggtgtgc gcgatcctgc
      721 tggcgttgcc tgtgaacctt tccgccagtg ggtaatagaa gataattttg ttgccggacg
      781 tccggaatgg gaaaaagcgg gagcagaact ggttagcgat gtactgcctt atgaagagat
      841 gaagttgcgc atgctcaacg gcagtcattc attcctggcg tatctgggtt atcttgctgg
      901 atatcagcac attaatgact gtatggaaga tgaacattat cgtcatgcgg cgtatggctt
      961 gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc aagattacgc
     1021 taaccgatta attgcacgct atagcaaccc ggcgttacgt catcgaacct ggcagattgc
     1081 gatggatggt agccagaaat tgccacagcg gatgttggat tctgttcgct ggcatctggc
     1141 gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg ggttggatgc gttatgtcgg
     1201 tggtgttgat gaacagggaa atccgataga aatcagtgac ccactgttac ctgttattca
     1261 gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc cagtcattgc tggcgattaa
     1321 ggcaatcttt ggtgatgatt tgccagacaa tagcttgttt actgcaaaag tgacggaagc
     1381 gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg gcgaaatatt ccgtgaagta
     1441 aaaaaagcaa tgccgccatc ggcggcattc acacacttag ttcatgccga gacgaatcag
     1501 gtccagaggt gccagtttct cttcacaacc ttcaacaact acggtttttc cgcggcggat
     1561 ttgctcagcg gtcagacctt cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc
     1621 gctaaccata acacggctgc cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat
     1681 aggtgttttc tcctttctga tttacagtcg aagcccacgt aacgggcgca atataaatac
     1741 tcctgtcgag tgaaatattt tttgtttttg atcaagctca catctttttt gatgcatgca
     1801 tgagccgtaa ggtgggggga aaagcctctc atcccggatg ggatgagagt aaatgaatta
     1861 ctcttcgcta aaccagtcgc tgttttcctg gcgaatctgg cgcacggatt cgctgatttc
     1921 ctgcaaatgt tgcgtcattg cacgttctac cgcatcgcca tcacgttttt gcagggcaga
     1981 gaaaatatca agatgctggc gtaacagcat ttctggtgga gaaacatggt cgaaactcat
     2041 atagcgcacg cgatcaacgg tcgctttcag gttctcaatg gtatcccacg ccagttgaca
     2101 gtcggcaatc tgcgtcagga gttgatggaa gttgtcatca agttcaaaaa aatcatccag
     2161 ttgcttgcgc tcaatggcaa tgcgttgctg gtgaagattt tgttccagtt ggtagcactg
     2221 gctttcggta atcatgctcg ccgcccgacg cgccaccgcg cactcaatgg cctgacggat
     2281 aaaactgccg tttcgcacct gggccatgga aattttgttg acgtagctgc cacgctgcgg
     2341 ccgaatttga atcaggccgt tttccgccag tttaataaag gcttcacgaa ccggctggcg
     2401 tgacacattg aaacgaacag aaacttcttt ttccgacaac ggtgtgcctg gtgcaatcag
     2461 gcaatggaca atgtcgcgac gaagaatacg ataaatctgc tgattgacag gctgtgtggg
     2521 attaagttgc gtttcgacgg tcattcgttc ttgcttgtga gtgagttaac tgcatgagtc
     2581 taccactttt ttgtgacaag caataacccg gcagttacgc cgggtataaa aattactgac
     2641 ggtggacatt cagtccggca tagctttggg taaccggcat catttccagg gtattgatat
     2701 tgacgtgagc aggcagcgtt gacacccacc agacggcttc gctgacatct tctggcgtca
     2761 atgcaacggt attttgatag gttttttccg ctttaccgtc atcgccttta aagcggacat
     2821 tggaaaactc ggtgccaccc accagacccg gttcgatgtc ggtgacgcgc accgccgtac
     2881 catgcagatc cgtacgcaga ttcaggctaa actgacgaac aaacgctttc gtcgcaccgt
     2941 aaacgttacc accggcatac ggccagctac ctgccgttga gccaatgtta ataatatgac
     3001 catgattacg ttcaaccata cccggtaaga cggcgcgcgt catatatacc aggcctttgt
     3061 tgttggtatc aatcatcgtt tcccagtctt caacgctggc tttatgcgca ggctccatgc
     3121 ccaacgccag gccggcatta tttaccagga tatcaatatt gcaccactcg gcagggagcg
     3181 atgccagcat ctcttcaata gcggcgcggt tgcgaacgtc cagttgggcg atatacagat
     3241 tatctcccag ttcgtctttt aactcctgca accgctcctg gcgacggcca gtggcgataa
     3301 ctttatgccc ttgttgaata aaacgacgag taatgcattc accaaaacct gccgttgctc
     3361 cagttactaa aacgatcatc tcactgttcc tcaacgcttt tgtgtgtcaa taacatagca
     3421 cgtgatgctt aagagcgggt aatactggtt tgcaagattg cactccgtta tcagtaagcc
     3481 tacgctatgg aacacctcgc gttcaggaga agaaaatgac aacaatgaat cctttccttg
     3541 tgcaaagcac actgccgtat ctggctcctc attttgatca aattgccaat catcactatc
     3601 gcccggcatt cgatgaggga atgcagcaaa agcgggcaga aattgctgcc atcgcgctta
     3661 acccgcaaac gcctgatttc aacaatacta ttctggcact ggaacaaagc ggagaattac
     3721 ttacccgcgt taccagcgtc ttttttgcga tgactgcggc gcataccaat gatgaattac
     3781 agcgtcttga tgaacagttt tccgctgaac tggcggaact ggctaatgat atctatctga
     3841 acggtgaatt attcgcgcgg gtagatgctg tctggcagcg ccgtgaatcc ctggggcttg
     3901 atagtgaatc catccgcctg gtggaggtta ttcatcaacg ttttgtcctt gccggagcca
     3961 aacttgcgca agctgataaa gcaaaattaa aagtactgaa tacagaagct gcgaccctga
     4021 ccagccagtt taaccagcgg ttacttgcag caaataaatc cggcggtctg gttgtgaacg
     4081 atatcgcgca gctggcagga atgagtgagc aagagattgc gctggcggca gaggcggctc
     4141 gcgagaaagg tctggataac aaatggctga ttccgctgct gaataccacc caacaaccgg
     4201 cgcttgccga aatgcgcgat cgtgcgacgc gtgaaaaact gtttattgcg ggctggacgc
     4261 gagcggaaaa aaatgatggc aatgataccc gcgctatcat tcaacgtctg gtagagattc
     4321 gcgcacagca ggcgaaactg cttggttttc ctcattatgc cgcatggaaa atcgccgatc
     4381 agatggcaaa aacacctgaa gcagcactca actttatgcg ggaaattgtt ccagcggcgc
     4441 gtcaacgtgc tagcgatgaa ttagcctcca tacaggcggt tatcgataag cagcaaggcg
     4501 ggtttagcgc gcagccgtgg gactgggcat tttatgccga acaggtacgg cgggagaaat
     4561 ttgatcttga tgaggcgcag ctcaagccat attttgaatt aaacacggtg ttgaatgaag
     4621 gtgtattctg gaccgcgaat cagctcttcg gtattaagtt tgtcgaacgt tttgatattc
     4681 ctgtctacca tcctgacgtt cgtgtgtggg aaatttttga tcataatggc gtgggactgg
     4741 cgttatttta cggtgatttc ttcgcccgtg attcaaaaag cggcggtgca tggatgggca
     4801 attttgttga gcaatcaacg cttaatgaaa cgcatccggt aatttataac gtttgcaatt
     4861 atcagaaacc cgctgccggt gagcctgcgt tgttactctg ggatgatgta ataaccttat
     4921 tccatgaatt tggtcatacg ctgcacggcc tttttgcccg ccagcgttat gccacgcttt
     4981 ccggcaccaa cacgccgcgt gattttgtcg aatttccgtc gcaaatcaac gaacactggg
     5041 caacgcatcc gcaggtattc gctcgctacg cccggcatta tcagagcggg gcagcaatgc
     5101 ctgacgaact gcaacagaaa atgcgtaatg ccagcctgtt caacaaaggg tatgagatga
     5161 gcgaactgct tagcgccgca cttctcgata tgcgctggca ttgcctggaa gaaaacgaag
     5221 caatgcagga tgtcgatgat tttgaattgc gggcgctggt ggcggaaaat atggatcttc
     5281 ctgctatacc gccacgctat cgcagcagtt atttcgccca tatttttggt ggcggatatg
     5341 ctgcaggtta ttacgcttat ctgtggacgc aaatgttggc cgatgatggt taccagtggt
     5401 ttgttgagca gggcggatta acgcgtgaaa atgggcagcg ttttcgcgag gcgatccttt
     5461 ccagaggtaa cagcgaagat ctggaacgcc tgtatcgaca atggcgcggt aaggctcctc
     5521 aaattatgcc gatgctgcaa catcgtggct tgaacatata aacaccacca atggctgtcc
     5581 tggagatatc gccaggccag cctggttata gcagtaattg cagcagctgg gcgagggcaa
     5641 acctcaccgc taaagctaat accgtttcgc agtcgccagc aaaatgcata accgcagtat
     5701 aggtctggcc tttaatatgc cacgcgaacc agacggtacc cgctggcgta ccatcttcac
     5761 cgccctccgg tccgccgtag ccggtaatgg caatactgac atcagcatcc gcacgctcta
     5821 tggcaccggt tgccatttct gctgccactt tctcactcac cgcagaatat cgttcaagag
     5881 attgctggct tacgctgagg attttcatct ttgcctgatc ggtgaaagta acaaagcctg
     5941 caccgtaaaa tttgggtgta tcttcagctg cacacagggc gctagccaac ttaccgccgg
     6001 tgcatgattc ggcggtagtt aagcgtaatt gatccgcgat aagtctttga ctcaacgcgg
     6061 atgtcaggtt ttcaatagtg tcggtatctg caagttgtac aattttatcg ctattaatgt
     6121 tcactgtttt ttgtccacgc ttttatatgt aaaggtgatt tgctttttct gtatgctcg
//
LOCUS       DAPPNJ010000229         6141 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000229 DAPPNJ010000000
VERSION     DAPPNJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.231"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            69..1289
                     /gene="astC"
                     /locus_tag="RPM73_003955"
     CDS             69..1289
                     /gene="astC"
                     /locus_tag="RPM73_003955"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDX6522255.1"
                     /translation="MSQPITRENFNEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTLNHRYGLFSEVRG
                     IGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            1286..2320
                     /gene="astA"
                     /locus_tag="RPM73_003956"
     CDS             1286..2320
                     /gene="astA"
                     /locus_tag="RPM73_003956"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDX6522256.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAEQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2317..3795
                     /gene="astD"
                     /locus_tag="RPM73_003957"
     CDS             2317..3795
                     /gene="astD"
                     /locus_tag="RPM73_003957"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDX6522257.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3792..5135
                     /gene="astB"
                     /locus_tag="RPM73_003958"
                     /pseudo
     CDS             3792..5135
                     /gene="astB"
                     /locus_tag="RPM73_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            5128..6096
                     /gene="astE"
                     /locus_tag="RPM73_003959"
     CDS             5128..6096
                     /gene="astE"
                     /locus_tag="RPM73_003959"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDX6522258.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1321 a         1559 c         1846 g         1415 t
ORIGIN      
        1 acatttacag cgcaaacatt acttattgtt aacatataaa taacgaatta tttactgtag
       61 aggtcgctat gtctcagcca attacgcgtg aaaactttaa tgaatggatg atacctgttt
      121 acgctccggc accctttata ccggtacgtg gcgaaggttc gcgcttgtgg gatcagcagg
      181 ggaaagagta tatcgacttc gcgggtggca ttgcggtgaa cgcgctgggc catgcgcatc
      241 cggaactgcg tgaagcgctg aacgaacagg cgagtaagtt ctggcatacc ggcaacggtt
      301 acaccaacga gccggtactg cgactggcga aaaaattgat cgacgccacg tttgccgatc
      361 gcgtcttctt ttgtaactcc ggtgcggaag ccaacgaagc ggcgctaaaa ctggcgcgta
      421 aattcgctca tgaccgctac ggcagccata agagcggcat cgtggcgttc aaaaatgcgt
      481 ttcatggtcg cacgctgttt actgtcagtg cgggtgggca gccagcctat tcacaggatt
      541 ttgcgccact gccaccggat attcgtcatg ctgcatataa cgatattaac tctgccagcg
      601 cgctgattga cgacgccacc tgtgcggtga ttgtcgaacc catccagggg gaaggcggtg
      661 tggtgccagc cagcaacgca tttttacaag gtctgcgtga attgtgtgac cgccacaatg
      721 ccttgctgat ttttgatgag gtacaaaccg gcgtcgggcg cactggtgag ctgtatgcct
      781 atatgcacta cggcgtgacg cccgatctgt taaccaccgc caaagcgctg ggcggcggtt
      841 tcccggtcgg tgcgttgtta gcaaccgaag agtgcgcccg cgtgatgacc gttggcactc
      901 atggcaccac ctatggcggc aatccgctgg cctcggcggt ggcgggcaaa gtgctggagc
      961 tcatcaacac accagagatg cttaatggcg ttaagcagcg tcacgactgg tttgttgagc
     1021 gtcttaatac ccttaatcac cgctacggtt tgttcagtga agttcgcggc ataggtcttt
     1081 tgattggctg tgtgctgaat gcggattacg ccgggcaagc gaaacagatc tctcaggagg
     1141 cggcgaaagc gggcgtgatg gtgctgattg ctggcggtaa tgtggtgcgt tttgcgcctg
     1201 cgctcaatgt cagcgaagaa gaggtgacga ccggactgga tcgctttgca gctgcttgcg
     1261 aacactttgt tagccgaggt tcatcatgat ggtcatccgt cccgttgagc gctcagatgt
     1321 ctcggcgcta atgcaacttg ccagcaaaac gggcggcggc ctgacgtcgc ttcccgccaa
     1381 tgaagccacg ctttcggcgc gtatcgaaag ggcaatcaaa acctggcaag gcgaactgcc
     1441 caaaagcgag cagggctatg tgttcgtgct ggaagatagc gagacaggca ctgtggcggg
     1501 gatttgcgcc attgaggtgg cggttgggct gaacgatccc tggtacaact atcgcgtcgg
     1561 cacgttggtt cacgcctcaa aagagctgaa tgtctataac gcattgccga cgctgtttct
     1621 tagtaacgat cacaccggca gcagcgagct gtgcacgctg tttctcgacc cggactggcg
     1681 caaagagggc aacggctatt tgctgtcgaa atcgcgcttt atgtttatgg cggcttttcg
     1741 cgacaagttt aatgacaaag tggttgctga aatgcgcggg gtgattgacg aacacggcta
     1801 ttcaccgttc tggcaaagcc tcggtaaacg cttcttttcg atggatttta gccgcgccga
     1861 ttttctctgc ggcaccgggc aaaaggcatt tattgcagaa ctgatgccga aacatccgat
     1921 ctatacccac tttttatccc aggaagccca ggacgtcatc ggtcaggtac atccgcaaac
     1981 cgcgcctgcc cgcgcggtgc tggagaaaga aggttttcgt taccgcaact atatcgacat
     2041 cttcgacggc gggccaacgc ttgagtgtga catcgaccgc gtgcgcgcca ttcgtaaaag
     2101 tcggctggtg gaagtggcag aagggcagcc tgcgcagggc gattttccgg cctgcctggt
     2161 cgccaatgaa aattatcacc atttccgcgt ggtgctggcg cgtaccgatc cggcaaccga
     2221 gcgtttgatt ttaaccgccg aacaactgga tgccctcaaa tgccacgcag gggatcgcgt
     2281 tcgtctggtg cgcctgtgcg cagaggagaa aacagcatga ctttatggat taacggtgac
     2341 tggataacgg gccagggcgc atcgcgtgtg aagcgtaatc cggtatcggg cgaggtgtta
     2401 tggcaaggca atgatgccga tgccgctcag gtcgaacagg cttgtcgggc agcccgtgcg
     2461 gcgtttccgc gctgggcgcg gctctcattg gctgaacgtc aggtcgttgt cgaacgcttt
     2521 gccggattgc tggaaaggaa taaaggcgaa ttaaccgcga ttattgccag agaaacgggt
     2581 aagccgcgct gggaagcggc aaccgaagtg acggcgatga tcaataaaat cgcgatatca
     2641 attaaggcgt atcacgttcg taccggcgag cagcgtagtg aaatgccgga cggtgcggcg
     2701 agcctgcgac atcgcccgca cggcgtgctg gcggtgtttg ggccgtataa tttccctggt
     2761 catttgccga acggacatat cgttccggca ttgctggcag gtaacaccat tatctttaaa
     2821 cccagcgaac tgacaccgtg gagtggcgaa gcggtaatgc gtttatggca gcaggctggc
     2881 ttgccgccgg gcgtactgaa cctggtgcag ggcgggcgtg aaacgggtca ggcgctgagt
     2941 gcgctggagg atctcgacgg tttgctgttt accggtagcg ccaataccgg ctaccagctg
     3001 catcgccagc tctccggtca gccggagaaa attctcgccc ttgagatggg cggtaataat
     3061 ccgctaatta tcgatgaggt ggcggatatc gacgcggctg tccatctgac cattcagtcg
     3121 gcgtttgtca cagccgggca acgctgcacc tgcgcccgcc gtttattgct caaaagcgga
     3181 gcgcagggcg atgcgtttct tgctcgtctg gttgccgtca gccagcgatt aacgccgggc
     3241 aactgggatg acgaaccgca gccgtttatt ggcgggctga tttctgaaca ggccgcacag
     3301 caggtggtta ctgcctggca gcaactggaa gcgatgggcg gacgaaccct gcttgcgccg
     3361 cgcttattac aatcagagac atcgttgctg acgccgggga tcattgaaat gacaggcgtt
     3421 gctggcgtac cagatgaaga ggtgttcgga ccgttattgc gcgtctggcg ttatgattct
     3481 ttcgaggaag cgattctaat ggcgaataac actcgcttcg gactctcttg cggtctggtt
     3541 tcccccgagc gggaaaaatt cgatcaactg ttgctggagg cgcgggcggg gattgttaac
     3601 tggaacaaac cgcttactgg tgctgccagt accgcgccat tcggcggcat tggtgcatcc
     3661 ggtaaccatc gccccagcgc ctggtatgcc gcagattact gcgcatggcc tatggcgagc
     3721 ctggagtcgg actcgttaac tttgcccgca acgcttaacc ccgggctgga tttttccgat
     3781 gaggtggtgc gatgaacgcc tgggaagtta atttcgacgg gctggtaggg ctgacgcatc
     3841 attacgctgg cctgtcgttt ggtaatgaag cctctacccg tcaccgtttt caggtgtcta
     3901 acccacgaca ggcggcaaaa cagggcttac tgaaaatgaa agcccttgcc gatgcgggat
     3961 tcccccaggc cgtgatcccg ccgcacgagc gtccgtttat tccggtgctg cgttagttgg
     4021 gattcagtgg tagcgatgag caggtactgg aaaaagttgc acgccaggca ccgcactggc
     4081 tttccagcgt cagttccgct tcgccaatgt gggtagccaa tgcggcaacg atcgcgccat
     4141 ctgccgatac gctggatggc aaagtgcatc tcaccgttgc caacctgaac aataaatttc
     4201 accgctcgct ggaagcgcac gtcaccgaat cacttttaaa agcgattttt aacgacgaag
     4261 agaaatttag cgttcattcg gcgttgccgc aggtagcgtt gctcggtgat gagggggcgg
     4321 caaaccacaa tcgtctcggc ggtcattacg gtgagccggg tatgcaactt tttgtctacg
     4381 ggcgagaaga aggcaatgat acccggcctt cccgttatcc ggcgcgacag actcgcgaag
     4441 ccagcgaggc ggtggcaagg ctgaatcagg tgaatcccca acaggtgatt ttcgcccagc
     4501 aaaacccgga cgttatcgac cagggcgttt ttcataatga cgtgattgcc gtgagtaacc
     4561 gccaggtgct gttttgccac caacaggcgt tcgctcgcca gtcacagtta ctggcaaacc
     4621 tgcgtgcgcg ggtgaacggt tttatggcga tagaagttcc ggcaactcag gtttccgtgt
     4681 cggatgcggt gtctacctat ctgtttaaca gccaactgtt gagccgcgat gatggttcca
     4741 tgatgttggt gctgcctcag gagtgtcggg aacacgccgg agtatggggt tatctcaatg
     4801 aactccttgc cgctgacaac ccgattagcg aactaaaagt ctttgattta cgtgaaagca
     4861 tggcgaatgg cggcggcccg gcgtgcctgc ggttgcgggt ggtattgaca gaagaagaac
     4921 gccgggcggt gaatccggcg gtgatgatga acgatacgct gtttaatgcg ctcaatgact
     4981 gggtggatcg ttactaccgc gatcgcctta ctgctgccga tctggccgac ccgcaattgc
     5041 tgcgcggagg gcgggaagca ctggatgtat tgagccaatt actgaatctc ggttcggttt
     5101 atccgttcca gcgcgaggga gggggcaatg gataattttc ttgctctgac cttaacgggt
     5161 aaaaaaccgg ttatcaccga gcgagaaatc aacggcgttc gctggcgctg gctgggcgat
     5221 ggtgtgctgg aactgacgcc attaacgcca ccgcaaggcg cactggtgat ttcagcggga
     5281 atacacggta atgagacggc acctgtggag atgctggacg cgttgcttgg cgcgatatct
     5341 cacggcgaga tcccgttacg ttggcggttg ctggtgatcc tcgggaatcc tcctgcgctg
     5401 aagcaaggga aacgttattg ccatagcgat atgaatcgaa tgtttggcgg tcgttggcag
     5461 ctatttgctg aaagcggaga aacctgtcgg gcgcgcgaac tggaacagtg cctggaagat
     5521 ttttatgacc tgggcaaaga atctgtgcgc tggcaccttg atctacatac cgcaattcgt
     5581 ggctccttgc atccgcagtt cggtgtatta ccgcaacgcg acattccctg ggacgagaaa
     5641 tttctgacgt ggctgggtgc ggcggggctg gaggcgctgg tgttccatca ggaacctggt
     5701 ggtacgttta cccatttcag cgccagacat tttggcgcgc tggcctgtac gctggaactt
     5761 ggcaaagcgt tgccctttgg gcaaaacgat cttcgccagt ttgcagtaac tgccagcgca
     5821 attgctgcgc tgctatctgg tgagagtgtc ggtatcgtga gaacaccgcc gctccgttat
     5881 cgggtggttt cgcaaattac tcgccactcg ccgtccttcg aaatgcatat ggcaagtgac
     5941 acgctgaatt ttatgccgtt tgagaaagga acattgctgg cgcaggacgg agaggaacgt
     6001 tttaccgtaa cccatgatgt agagtatgtg ttattcccta atccgttggt agcgttggga
     6061 ttacgcgcgg gattaatgct cgaaaaaata agctaatact tacccgcaga aatcattctg
     6121 cgggtattaa gttgctttct t
//
LOCUS       DAPPNJ010000230         6119 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000230 DAPPNJ010000000
VERSION     DAPPNJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.232"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..202
                     /locus_tag="RPM73_003960"
     CDS             <1..202
                     /locus_tag="RPM73_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDX6522259.1"
                     /translation="AINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLA
                     MLFTLANLFRVDQMIRQWERSQ"
     gene            1200..1871
                     /locus_tag="RPM73_003961"
     CDS             1200..1871
                     /locus_tag="RPM73_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522260.1"
                     /translation="MADWFIATEGVKVVKDSASLWPQIVTAISSAGAALVGVALTHRF
                     TRRREEATAARKRYDELYFISTELVFLLERFAQRCVYSANESGEYDQDGRIRIEYSLP
                     EINFESITGDWRSLPPGLMYRLAELPVICREAASSISAAFNEDTPFDGSYGIFELNRQ
                     SARLGLKAIRLSRWLRQLCDMPGDRLSDDRWSAWSVLYRKRWEHIRNNNNVHRRYKRL
                     PDVTE"
     gene            1891..2679
                     /locus_tag="RPM73_003962"
     CDS             1891..2679
                     /locus_tag="RPM73_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDX6522261.1"
                     /translation="MNNNFPAGIRDNTRNTSMNLPVAIDYPAALALRQMALVQDELPK
                     YLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRRDFSLAPPYPFVQLATL
                     KQRTEKAARTAGRAPAGSQVHRLVPLSDHHYVSQLQMMVATLKIPLERRNKRTGRMEK
                     ARIWEITDRTVRTWLSEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQS
                     LMGHKSISSTEVYTKVFALDVAARHRVQFQMPEADAVAMLKGNI"
     gene            complement(2737..2991)
                     /locus_tag="RPM73_003963"
     CDS             complement(2737..2991)
                     /locus_tag="RPM73_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522262.1"
                     /translation="MTLKCLLNPWRFSGLNSFTDGVIIRDTKVLITLNLPDGQKTTIE
                     GEWRHMSDANRLSERITMLQHAIYEQYEEYRADIPVSWSR"
     gene            3018..3266
                     /locus_tag="RPM73_003964"
                     /pseudo
     CDS             3018..3266
                     /locus_tag="RPM73_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3276..4324)
                     /locus_tag="RPM73_003965"
                     /pseudo
     CDS             complement(3276..4324)
                     /locus_tag="RPM73_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            4389..5285
                     /locus_tag="RPM73_003966"
                     /pseudo
     CDS             4389..5285
                     /locus_tag="RPM73_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5387..5668
                     /locus_tag="RPM73_003967"
     CDS             5387..5668
                     /locus_tag="RPM73_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522263.1"
                     /translation="MIYKDITILYIDSDKNNRLIRYDLLRKENNDFVVQVFDDQNEDI
                     ADPKPTIKIDQFEITYDNYLDNCKHSNKLPASFEEYVDIKLQDHRDKLD"
     gene            complement(5769..>6119)
                     /locus_tag="RPM73_003968"
     CDS             complement(5769..>6119)
                     /locus_tag="RPM73_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDX6522264.1"
                     /translation="AKSRQRWLFYAYDRLRKTVVAHVFGERTLATLERLLSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKIIGHYLNIKHYQ"
BASE COUNT         1563 a         1465 c         1486 g         1605 t
ORIGIN      
        1 ggccatcaac atcgaataca tgaaagccag catccgtgcc agggtggagc acccgtttcg
       61 catcatcaag cggcagttcg gcttcgtgaa agccagatac aaggggctgc tgaaaaacga
      121 taaccaactg gcgatgttat tcaccctggc caacctgttt cgggtggacc aaatgatacg
      181 tcagtgggag agatctcagt aaaaaccgga aataacgcca gaaatggtgg aaaaaatagc
      241 ctaaacaggc cgattcgatg tgtttgcggg aaaaaaatcg gcccagatcc gcgaaatttt
      301 aatcagcgag tcagcttggg aagaaatgac ctgcttattc gcaccttccc tagtagtaat
      361 tacaaaaatt gcttttatgc tttttaagca ttgcttttat atttttaata atgtcagttt
      421 ttatccctca ctcctttctt ttccagagat ccgaaaaaag taagtcccgg atctctgcga
      481 accccaccgg cgcgcagcgg cccgacaaca caagatgtag cggtgtttcc agctgatagt
      541 gacgccatat gttgtgtccc tctcctgccc cgtgacccgg cgcgcagcat cagaaaacag
      601 tgcttcgctt acttcctgac cgtggtaaac ttccggaaac ttcgctaaat gtgaaaagtc
      661 ggatctgccg gaaacggtca ttcggtaatt taacagcgtt gctatatggc tgcttattat
      721 cagtcaaaaa aataattaca gtatgggaaa ttcatccagg tgacagatgc agtattgtgc
      781 tcaactaatt ataccttgcg tcataattac ccttagtgac tatttgttag gaggctaaat
      841 gcacgcatca atggttcatg tctgggagta tctgacacgt tgtggtcata aattcctatg
      901 gctaaaaatt aaccctctac ctccaggata taccggttcg gtatacagag cgcagtttga
      961 aatttacagc gcgtcgggcc gtattctggt cacgcttgat gtgcacagac agcaatgggt
     1021 gtgttcagca aacaatcctc gccgtgaacc tgctgataaa aacggggtca tgcttgatag
     1081 cggtatacct ctggatagaa acctcgcatc acattatgca aaacaggggt actgaatgaa
     1141 tctaaccctg gtacaagcac tggcagatgc ctggcgggat ttctcaataa ggatcaaaca
     1201 tggctgactg gtttattgcg actgagggcg tgaaggtggt gaaggacagt gccagcctgt
     1261 ggccgcagat cgttacggct atttcctctg ccggcgcagc cctggtgggt gttgctctca
     1321 cacaccgttt tacccgcagg cgtgaagaag ctactgctgc acgtaaacgt tacgatgaat
     1381 tgtactttat atctaccgag ctagtttttc tgctggagcg ttttgcacaa cgctgtgttt
     1441 attctgctaa tgaatcaggg gaatatgacc aagatggacg tattaggatt gagtattctt
     1501 tacctgaaat caatttcgag tccattactg gggactggcg ttctctgcca ccaggattga
     1561 tgtacagact tgctgaattg ccagtcatct gcagggaagc cgcgagttct ataagtgcgg
     1621 cgttcaatga agatactccc ttcgatggat cgtatggaat tttcgaactt aacaggcagt
     1681 ctgcaagact cggattaaaa gcaatacgtt tatcccgttg gctacgtcag ctatgcgata
     1741 tgcctgggga caggttgtct gacgaccgtt ggtcggcatg gagtgttttg tatcggaagc
     1801 gctgggagca tattaggaat aataacaatg ttcaccgccg atataaacgg ttgcctgatg
     1861 ttactgagta gatggctcag gagttagact atgaataata atttcccggc aggtatcagg
     1921 gataacacac ggaacacctc gatgaatctg ccagtagcca tcgattaccc tgccgctctc
     1981 gccctgcgtc agatggcgct cgtgcaggat gaactcccga aatacctgct ggcgccggaa
     2041 gtcagcgccc tgctccatta cgtgccggat cttcaccgca agatgctgct ggcgacgctg
     2101 tggaacaccg gcgcgcgcat taatgaagcg ctcgcgctga cccggaggga tttttcgctg
     2161 gcgccgccgt atcctttcgt gcagctggcc acgctcaagc agcgcaccga gaaagcggcc
     2221 aggacggcag gtcgtgcccc cgcaggcagt caggtccatc gtctggttcc cctttccgat
     2281 caccactacg tcagtcagct gcagatgatg gtggccaccc tgaaaattcc cctcgaaaga
     2341 cgaaacaaac gcaccggcag aatggagaag gcgcgtatct gggagatcac agaccgaacc
     2401 gtccgcacct ggcttagcga agcggtggaa gcagcagcgg ccgacggggt gacgttctca
     2461 gtaccggtga ccccccatac gttccgtcac tcctacgcga tgcacatgct gtatgccggc
     2521 ataccactga aggttttgca gagcctgatg ggccacaaat cgattagctc gacggaagtc
     2581 tatacgaagg tgtttgcact tgatgttgct gcgcggcatc gagttcagtt tcagatgccc
     2641 gaagcggatg cggtagccat gctgaaaggg aatatttaag tacttaggag aatgatgctg
     2701 gcgaacgtca tgctcgccgg tgaggtctgt cagaagttat ctggaccagc tgacgggaat
     2761 atctgctctg tattcctcat actgctcata gatcgcatgc tgaagcatcg taatacgttc
     2821 tgacaacctg ttggcgtccg acatatgccg ccattccccc tcaatagtgg ttttctgtcc
     2881 atccgggaga ttgagcgtga tcaatacctt tgtatcacga ataatcaccc cgtcagtgaa
     2941 agaattaaga ccactgaatc gccacgggtt taacagacac ttcagagtca tttaagatgg
     3001 cttaaagaga ggtgcccatg agcggtaagc gttatcccga agagtttaaa actgaagcag
     3061 tcaaacaggt tgttgatcgc ggttattctg ttgccagcgt tgcaacacgt ctcgatatca
     3121 ccacccacag cctttatgcc tggataaaga agtacggtcc ggattcttcc actaataaag
     3181 aacagtcaga tgctcaggcc gagatccgcc gtctccagaa agagctgaaa cgggttaccg
     3241 acgaacggga catattaaaa aaagccgctg tagattcaat ctgtcaatgc aacacccctt
     3301 tcaattatct ctttcggtgt tttgaacttc agtgtctttc tcggtctgtt gtttagctga
     3361 gcagcaacca gatctagttc atgttgagta tattgggcaa gacatgtctt tttaggaaag
     3421 tactgccgaa ttagcccatt tgtgttctca tttgttcccc gctgccaagg actctgagga
     3481 tcgcagaagt aaactttaac gccggtgctg acagtaaatt ctagatgtct ggccagttcc
     3541 attcctctgt cccatgtcag tgattttctg agttctgacg gtaaactcag gaatttgtcg
     3601 gtaagagcct gatttactga gacagaatct ttgcccctga gtctaaggat gatcgtataa
     3661 cgtgattttc ggtctacaag tgtggctata tgagagtttt ttgtacctga gactaaatcg
     3721 ccctcccaat gccccagaga gcgtctgtta tcgatatttc gggaacgttc gtgaattggt
     3781 gtagcgcttc acggctacga aagtacagcg ttttataaat tgtctcaggt gatattcgca
     3841 gcgttttttg acgtggtttt gttcgcctta accatcctga tatttgctct ggagaccatt
     3901 tcatctccag cttttccaga acaagctttc gcaatggtaa attttgatcc agtaagcacg
     3961 gttttggcct tttcgccatt ctgttggctc ggttattagc atcaacagct ttgtaatagc
     4021 gtctgccccg attacgctga acttcacgtg agatcgtcga aggactgcga ttcagcgcag
     4081 tagctatcgc acgaatgctc attttggctg acaaaccagc tcgtatctcc tcgcgctcag
     4141 acagtgtcag gtgagctaca gcccgcttac gctcatgggg ttttatgccg ccagtatccc
     4201 ttaacatagt gaagatcgtt gcgggttttg aacccaggat attcgctatt tcactgaagc
     4261 ctgttccgtt cttccatagt tcaaaaacag aggctttttc ctctgctgta aatgttcgtc
     4321 tcattcaaaa aacctccgca accccatgtt ttcacataac tgttgcgttg accaattgaa
     4381 tctacagcgg cgtacttcgc aaagctgtcc gactgaggta cgcctttatc cgtgacaact
     4441 cctgttgctg gcctgttcgc ctgctctgtc gggtgctgga tgttcatccc agtggttttt
     4501 acgcctggct tcagcagccg cattcacaac gccatcaggc agacctgaga ctgacaggac
     4561 agattaaaca gttctggctg gaatcgggat gcgtctatgg ttatcgcaaa atccatctgg
     4621 atctgcgtga cagcgggcaa cagtgcggag taaacagagt ctggagactg atgaaacgtg
     4681 tcggaataaa ggctcaggtc ggataccgaa gcccgcgggc acgtaaaggc gaggccagta
     4741 tcgtgtcacc caacaggctc cagcgacagt tcaatccgga tgctcctgat gagcgttggg
     4801 taacggacat aacctacatc aggacccacg aaggctggct gtatcttgcc gttgttgttg
     4861 atctgttctc acgcaaaatt atcggctggt ccatgcaatc ccggatgaca aaggacattg
     4921 tcctgaacgc actgctgatg gctgtatggc ggcgtaatcc cgaaaaacag gtgctggttc
     4981 attcggatca gggcagtcag tacacaagcc atgagtggca gtcgttcctg aaatcacacg
     5041 gcctggaggg tagcatgagc cgtcgcggta actgccatga taatgcggtt gcagaaagtt
     5101 ttttccagtt gttgaaacgt gaacggataa agaaaaagat ctacggaacg cgggaagaag
     5161 cccgcagcga tatttttgat tacatcgaaa tgttttataa cagtaagcgt cggcatggtt
     5221 ccagcgatca gatgtcaccg acagaatatg aaaaccagta ttatcaacgg ctcggaagtg
     5281 tctagattat ccgtggcgat tcattttgtg ttacaattca gtatatacca gtttagcact
     5341 cctgttagat tggtgtgtat cacactgaca caatgaggtt ttttctatga tttataaaga
     5401 tataactatt ctttacatcg atagtgataa aaacaatcga ctcatccgtt atgatttact
     5461 gcgaaaagaa aataatgact ttgttgttca agtgtttgat gaccagaatg aagacattgc
     5521 tgatcctaaa ccaacaataa aaattgatca gtttgagatc acttatgata attaccttga
     5581 taattgcaag cattccaata agttaccggc ctcatttgaa gaatatgtcg acataaaatt
     5641 acaggaccac cgggataagc ttgattaagc cacatacatg tttagcttaa ggctcagaac
     5701 attttaaatg tctgagccat ttctcacaga cccctaccct gtaatggcca tcgggtaatg
     5761 actccaactt attgatagtg ttttatgttc agataatgcc cgatgatttt gtcatgcagc
     5821 tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga
     5881 ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc
     5941 ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt
     6001 gacagcaggc tcagaagacg ctccagtgtg gccagagtgc gttcaccgaa aacgtgcgca
     6061 acaaccgtct tccggagcct gtcatacgcg taaaacagcc agcgctgacg tgatttagc
//
LOCUS       DAPPNJ010000231         6101 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000231 DAPPNJ010000000
VERSION     DAPPNJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.233"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            229..510
                     /locus_tag="RPM73_003969"
     CDS             229..510
                     /locus_tag="RPM73_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDX6522265.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(609..1145)
                     /gene="ssb1"
                     /locus_tag="RPM73_003970"
     CDS             complement(609..1145)
                     /gene="ssb1"
                     /locus_tag="RPM73_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDX6522266.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1399..4221
                     /gene="uvrA"
                     /locus_tag="RPM73_003971"
     CDS             1399..4221
                     /gene="uvrA"
                     /locus_tag="RPM73_003971"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDX6522267.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4256..4612)
                     /locus_tag="RPM73_003972"
     CDS             complement(4256..4612)
                     /locus_tag="RPM73_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDX6522268.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4616..5032)
                     /locus_tag="RPM73_003973"
     CDS             complement(4616..5032)
                     /locus_tag="RPM73_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDX6522269.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5143..5856)
                     /gene="aphA"
                     /locus_tag="RPM73_003974"
     CDS             complement(5143..5856)
                     /gene="aphA"
                     /locus_tag="RPM73_003974"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDX6522270.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1445 a         1586 c         1583 g         1487 t
ORIGIN      
        1 tatgccctta atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta
       61 atatggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa caaagagagt
      121 gtttcatttt tgtgttctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa
      181 caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc
      241 accactggcg tggttcttct tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc
      301 agcacactca acatccgttt tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc
      361 ggtctgggcg ttgtttccgc ctgttggttc gcaatggggt tgcttggcat cacaatggcg
      421 gacatcaccg ctatctggca caacatcgag tcggtgatga tagaagagat gaatcagaca
      481 ccgccacaat ggccaatgat tttgacctga tacgtagaag cctccataac ggaggcttct
      541 tttttatggc tggcagatgc tttaatcatc caccttaaaa caatataacc tattgtttta
      601 atgacaaatc agaacggaat gtcatcatca aagtccatcg gcggctcgtt agacggcgct
      661 gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccaccctgc
      721 ggctgctgtg gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgcc
      781 ggagcgccac caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg
      841 acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc atttacgggt acgcagctga
      901 ccttcgatat aaacctgaga acctttacgc agatattcgc tcgccacttc tgccagtttg
      961 ccgaacagca caacgcggtg ccattcagtc tgttctttca tctcgccggt cgctttatca
     1021 cgccaggatt cggaagtagc cagcgtaatg ttggcaactg cgccaccatt tggcatgtag
     1081 cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg
     1141 gccatgttcg tgtctcctga aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc
     1201 attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga
     1261 cattgttaca caacactccg ggtaatgcat tccaatactg tatattcatt caggtcaatt
     1321 tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt
     1381 ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc
     1441 aaaaacatca acctcgttat cccccgcgac aagctcattg tcgtgaccgg gctttcgggt
     1501 tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt
     1561 gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat
     1621 catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg
     1681 cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc
     1741 gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag
     1801 atggtggata acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct cgcgccaatc
     1861 attaaagagc gcaaaggcga acacaccaaa acgctggaga acctggcaag ccagggttac
     1921 atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccgaa actggaactg
     1981 caaaagaaac acactattga agtggtggtt gatcgcttca aggtgcgtga cgatcttacc
     2041 caacgtcttg cggagtcgtt tgaaaccgcg ctggagcttt ccggtggtac agcggtagtg
     2101 gcggatatgg acgacccgaa agcggaagag ctgctgttct ccgccaactt cgcctgccca
     2161 atttgcggct acagtatgcg tgaactggag ccgcgactgt tttcgtttaa caacccggca
     2221 ggtgcctgcc cgacctgtga cggccttggc gtacagcaat atttcgatcc tgaccgcgtg
     2281 atccaaaacc ccgagctgtc actggctggc ggtgcgatcc gtggctggga tcgccgcaac
     2341 ttctattatt tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg
     2401 ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa
     2461 aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg tcatccgttc
     2521 gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga cagaatccag tgcggtacgt
     2581 gaagaattag ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg gacgcgtctg
     2641 cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat ctccgacatg
     2701 agcatcggtc atgcgatgga attcttcaac aatctcaaac tcgctggtca acgggcgaag
     2761 attgcagaaa aaatccttaa agagatcggc gatcgtctga aattcctcgt taacgtcggc
     2821 ctgaattacc tgacactttc ccgctcggca gaaacacttt ccggcggtga agcccagcgt
     2881 atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtatgt actggacgag
     2941 ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt tgggtacgct tatccatctg
     3001 cgcgatctcg gtaataccgt gattgtggtg gagcatgacg aagacgcaat tcgcgccgct
     3061 gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg gcggtgaagt ggtcgcagaa
     3121 ggtccgctgg aagcgattat ggcagtgcct gaatcgttga ccgggcagta catgagcggt
     3181 aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccagaaaa agtgctgaag
     3241 ctgacaggtg cacgcggcaa caacctgaag gacgtgacgc tcacgctgcc agtcggtctg
     3301 tttacctgca tcacaggggt ttcaggttcc ggtaaatcga cgctgattaa cgacacactg
     3361 ttcccgattg cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc accgtatcgc
     3421 gatattcagg ggctggagca tttcgataaa gtgatcgata tcgaccaaag cccaattggt
     3481 cgtactccac gttctaaccc ggcgacctat accggcgtgt ttacacctgt gcgcgaactg
     3541 tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc caggacgttt cagctttaac
     3601 gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt ggagatgcac
     3661 ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag gtaaacgcta taaccgtgaa
     3721 acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa
     3781 gaggcgcgtg agttctttga tgcggtgcca gctctggcgc gtaagctgca aacgttgatg
     3841 gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa ccacactttc tggtggtgaa
     3901 gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat
     3961 attctcgacg agccgaccac cggtctgcac ttcgccgata ttcagcaact cctcgacgtg
     4021 ctgcataaac tgcgcgatca gggcaatacc attgtggtga ttgagcacaa tctcgacgtg
     4081 atcaaaaccg ctgactggat tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa
     4141 atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc
     4201 ttcctcaagc cgatgctgta atcgttaagg ccgctttctg agcggccttt tcctttcaga
     4261 gttgtaccag caatttacgc ttttcttccg gtagtaaatt cactgcctgc tgataagacg
     4321 catccaccag ataatagatt tgcgaatccg gcagcgaacc gtcgagatac acggtgctcc
     4381 agtgcgcttt attcagatgg cggctaggac gcacatcgct gtgctgctga cgtagcagct
     4441 ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg attttctact tctttcacca
     4501 tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct
     4561 gttctgcgcc tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact
     4621 ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc
     4681 agatgccttg ccaggtgccg gtctgaatac gccctttatg taacggcaat acaagcgatg
     4741 ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct
     4801 cataatttcc gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg
     4861 tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca
     4921 gtaagccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca
     4981 gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct
     5041 ccttgataaa actctcttaa tcagtttgca gcaagacagc gaaaggataa aggtgtgatt
     5101 aaaaaaacag cattcgggag agtgtcacgc tctcccgctc tgtcagtatt ctgaattgac
     5161 gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc
     5221 gcgcagaatg cggataccac gagcgccgac atcgcgtgca gcggtaatat cgttatcgga
     5281 atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc
     5341 tggtttatcg cccgcaaaga tcaccggatt catgttggtg acaggaatat gaaaattatc
     5401 cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca caaagaagat
     5461 cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct
     5521 gaattcatcc cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc
     5581 gctttctggt gagaaggttt ttttgccgcg ccagaagccc gggctggaaa aaagtaccgt
     5641 gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg
     5701 tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt tagtcccagg
     5761 gttaagcggt gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa
     5821 gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg
     5881 ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg ccagctgccg
     5941 gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta tgtttaaccc
     6001 agtaaatacg ttcctgtctt tttttgtata aaaaatgttt attactcgat gtaaggatac
     6061 attaatttta cgtttattat gatgaatcct ctgcaaatga g
//
LOCUS       DAPPNJ010000232         6099 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000232 DAPPNJ010000000
VERSION     DAPPNJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.234"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            67..843
                     /gene="exuR"
                     /locus_tag="RPM73_003975"
     CDS             67..843
                     /gene="exuR"
                     /locus_tag="RPM73_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDX6522271.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1188..1850
                     /locus_tag="RPM73_003976"
     CDS             1188..1850
                     /locus_tag="RPM73_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX6522272.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1854..2237
                     /gene="mzrA"
                     /locus_tag="RPM73_003977"
     CDS             1854..2237
                     /gene="mzrA"
                     /locus_tag="RPM73_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDX6522273.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGITMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2384..2752
                     /locus_tag="RPM73_003978"
     CDS             2384..2752
                     /locus_tag="RPM73_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDX6522274.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2790..3095
                     /locus_tag="RPM73_003979"
     CDS             2790..3095
                     /locus_tag="RPM73_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDX6522275.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3098..3502
                     /locus_tag="RPM73_003980"
     CDS             3098..3502
                     /locus_tag="RPM73_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDX6522276.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3492..3791
                     /locus_tag="RPM73_003981"
     CDS             3492..3791
                     /locus_tag="RPM73_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDX6522277.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3977..4369
                     /locus_tag="RPM73_003982"
     CDS             3977..4369
                     /locus_tag="RPM73_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDX6522278.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4439..5425
                     /gene="yqjG"
                     /locus_tag="RPM73_003983"
     CDS             4439..5425
                     /gene="yqjG"
                     /locus_tag="RPM73_003983"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDX6522279.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5718..6083
                     /locus_tag="RPM73_003984"
     CDS             5718..6083
                     /locus_tag="RPM73_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX6522280.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1522 a         1511 c         1541 g         1525 t
ORIGIN      
        1 ccaatggcat acccatgaat aaagtggtat gataacttcg tccgcattcc actccgggag
       61 acgcatatgg aaatcactga accacgccgt ttgtatcaac aacttgccgc tgacctgaaa
      121 gagcgcatcg aacagggcgt ctatctggta ggtgataaac tgcctgcaga acgctttatt
      181 gccgatgaaa agaacgtcag ccgtacggta gttcgtgaag ccatcatcat gctggaagtt
      241 gaaggctatg tcgaagtgcg taaaggttca ggtattcatg tggtttccaa ccagccgcgc
      301 catcagcagg cggctgacaa taatatggaa ttcgccaatt acggtccgtt tgagttgctt
      361 caggctcgcc agctcatcga aagtaatatt gccgagttcg cggcaactca ggtaacgaaa
      421 caagacatca tgaaactgat ggccattcag gaacaggcgc gcggcgaaca atgcttccgt
      481 gattccgagt gggatttaca gttccacatt caggtagccc tggcgacgca gaactccgcc
      541 ctggcggcca tcgttgaaaa aatgtggacc caacgtagcc ataacccgta ctggaaaaaa
      601 ctgcacgaac acattgattc ccgtaccgtc gataactggt gtgatgacca cgatcaaatc
      661 ctcaaggcgc tgattcgcaa agatcctcat gctgctaagc tggcaatgtg gcagcacctg
      721 gaaaacacca agatcatgtt attcaacgaa accagcgacg acttcgagtt caatgccgac
      781 cgctatctgt tcgccgaaaa cccggtagtg catctcgata cggccaccag tggcagtaaa
      841 tgaaactcct gtccgacagg cgctgcgacg cgcctgtttg tctcgtcggt aagcgaaaag
      901 tataaagtgt cagcctgtgt aaatcctctc gccaccctcc cctgcattca gcaaaatcag
      961 actccacgga catgtaattt tgataacgaa caacgttgac ctttgttaca attagattca
     1021 atttgaattt atgtttttga atgctttctt atctcacgat ttaacaggga atagttcagg
     1081 ctgtgttgat gtatcaaacc cgcagaacat accaaaacag caataacatt gcggtagtgc
     1141 atcttttaaa accagcgtgg cgttaaccga ttcaccagga ataatgaatg gaacttttga
     1201 cccaattgct gcaagccctg tgggcgcagg attttgaaac cctggccaat ccatcgatga
     1261 ttggcatgtt gtattttgtc ttgtttgtaa ttttgttcct tgaaaacggc ttgcttccag
     1321 cggccttttt accgggcgac agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg
     1381 cgatgggtta cccgcaaacg attctgctgc tgaccgttgc cgccagcctc ggctgctggg
     1441 tcagctatat tcaggggcga tggctgggca atacccgcac cgtacaaaac tggctatctc
     1501 atttacccgc gcattatcat caacgcgcac accatctttt tcataaacac ggtttatcgg
     1561 cgctgttaat tggtcgcttt attgcgtttg tcagaacact gctgccgacg attgccgggt
     1621 tatcagggct gaataacgcg cgctttcagt ttttcaactg gatgagcggt ctgctgtggg
     1681 tattgatcct gacaactctg ggttacatgc tcggcaaaac gccggtattt ttaaagtacg
     1741 aggaccagct gatgtcatgc ctgatgctgc tcccggtggt gctgctggtg tttggcctgg
     1801 caggttctct ggtcgtgtta tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa
     1861 tacctcgcat gtcgcttcgc cagctagcct ggtccggcac tgttttactt ctggtcggca
     1921 cgctgttgct ggcctggtcc gcggttcgcc agcaagagtc tacgctggcg attcgtgccg
     1981 ttcatcaagg cataacgatg ccagacggtt tttcgatctg gcatcacctt gacgctcatg
     2041 gcattccttt caaaagtatc acccccaaaa acgacactct gttaattaca tttgactcca
     2101 gcgaccagag cgccgccgca aaagcggtcc tcgacagaac attgccccat ggctacatca
     2161 ttgcgcagca ggacaataac agtcaggcta tgcagtggct gacccggtta cgggataact
     2221 ctcatcgctt cggataactt cctgttatcc gaaacatatc actcactttg gtgatttcac
     2281 cgtaactgtc tatgattaat gaggcggtgg ttaacactac cgcttcgttg tctggacaat
     2341 cgttcctttg taataggtcc gaatcacaat ggaaggttca agaatgaaat accgcatcgc
     2401 tttagctgtt tctctctttg ctcttagtgc cggtagttat gccactaccc tgtgtcagga
     2461 aaaggagcaa aatatcctta aggagatcag ctatgccgaa aaacaccaaa accagaatcg
     2521 tattgacggt ctgaataaag ccctgagtga agtccgggcc aactgttcag atagccagct
     2581 gcgtgccgat catcacaaga aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca
     2641 agatttagcc gaggcgaagc aaaaaggcga tgccgataag attgccaaac gcgaacggaa
     2701 actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact aactcacaat
     2761 agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg gaacatctgc
     2821 gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca tctggcgaga
     2881 agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg aaacagagcc
     2941 gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc
     3001 gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc gctgcaatcg
     3061 gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc atcacgcgca
     3121 agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca tggttgaaat
     3181 ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag ctaatctctt
     3241 tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc ttatgagcct
     3301 gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga tgattgccac
     3361 caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac gtaaatcgcg
     3421 taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc aactgctcga
     3481 ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc
     3541 agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg gaggcaacag
     3601 gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg ctggttggca
     3661 gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc tgggccagac
     3721 gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag cagcaacagc
     3781 ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa attttttgaa
     3841 gatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga ttctctccat
     3901 cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca gcctgagtgg
     3961 cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag cgcgcatttt
     4021 aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact ctggtaccca
     4081 acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga ttctgcttga
     4141 gttcggtggt ggtctggcaa tcctgttcgg tttcctgact cgcaccacag ccctgtttac
     4201 tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg aaggcgtcaa
     4261 ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc tggcaattac
     4321 cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag cacgcgctat
     4381 acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg agagaaaaat
     4441 gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat ctaccggcgg
     4501 taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg gcgcacctgg
     4561 ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct atgtttcact
     4621 cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt
     4681 tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct ttgatgacag
     4741 ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc agctttatct
     4801 ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg acaaaaagaa
     4861 ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata ccgcgtttga
     4921 tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga aaattgacga
     4981 acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg gttttgccac
     5041 cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga
     5101 gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag ccgatattcg
     5161 cctgtggacc acgctggtcc gttttgatcc agtgtatgtg acccacttca agtgtgataa
     5221 gcaccgcatc agcgattacc ttaatctgta tggcttcctg cgcgatatct accagatgcc
     5281 gggaattgcc gaaacagtca atttcgatca catccgtaat cattacttcc gcagccataa
     5341 gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg atgaaccgca
     5401 tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc tgataattag
     5461 ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacat
     5521 ttatattcaa tatgttgcaa tgaccgcgca ggccagataa gcgtagcgca tctggcgatt
     5581 ttgcgcttgt catcagtctt agcgcgcctt cacagcactt tattaattcc ttgaacgaat
     5641 atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac aagtgattga
     5701 gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat gtcggtttcc
     5761 ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc atctttacgt
     5821 tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc gaaggcatcc
     5881 tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcagttcag ttccgccgcc
     5941 tgcacgacac cgaccgctcg gcgtggtggg cactgttgtt cttaatcccg tttatcggct
     6001 ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac cgctttggac
     6061 cagatccgaa actggaacct taaaacaaat ctgctcgcc
//
LOCUS       DAPPNJ010000233         6091 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000233 DAPPNJ010000000
VERSION     DAPPNJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.235"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(51..347)
                     /locus_tag="RPM73_003985"
     CDS             complement(51..347)
                     /locus_tag="RPM73_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDX6522281.1"
                     /translation="MKTMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="RPM73_003986"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="RPM73_003986"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDX6522282.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="RPM73_003987"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="RPM73_003987"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDX6522283.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="RPM73_003988"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="RPM73_003988"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDX6522284.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5270..5389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="RPM73_003989"
     CDS             complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="RPM73_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDX6522285.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5604..>6091)
                     /locus_tag="RPM73_003990"
     CDS             complement(5604..>6091)
                     /locus_tag="RPM73_003990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDX6522286.1"
                     /translation="LHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALV
                     FGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQQPAKSDTTA
                     DQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNIT
                     K"
BASE COUNT         1442 a         1691 c         1498 g         1460 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatcg ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagccctg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgattgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
     2641 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
     2701 acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
     2821 ggcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcgg caggcaccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgagcgaga atcaatagct tacgcgctac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgcgccag agtagtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caagaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagcccg
     4141 cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc
     4681 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
     4981 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
     5281 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
     5341 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
     5401 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
     5461 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
     5521 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
     5581 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
     5641 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
     5701 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
     5761 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
     5821 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
     5881 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
     5941 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
     6001 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg
     6061 gtggcgacag tgaaatcgac atcgtgtaac g
//
LOCUS       DAPPNJ010000234         6048 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000234 DAPPNJ010000000
VERSION     DAPPNJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.236"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            157..1260
                     /gene="ompC"
                     /locus_tag="RPM73_003991"
     CDS             157..1260
                     /gene="ompC"
                     /locus_tag="RPM73_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDX6522287.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1372..2427
                     /gene="apbE"
                     /locus_tag="RPM73_003992"
     CDS             1372..2427
                     /gene="apbE"
                     /locus_tag="RPM73_003992"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDX6522288.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2501..3565
                     /gene="ada"
                     /locus_tag="RPM73_003993"
     CDS             2501..3565
                     /gene="ada"
                     /locus_tag="RPM73_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDX6522289.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3565..4215
                     /gene="alkB"
                     /locus_tag="RPM73_003994"
     CDS             3565..4215
                     /gene="alkB"
                     /locus_tag="RPM73_003994"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDX6522290.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4291..5934
                     /gene="yojI"
                     /locus_tag="RPM73_003995"
     CDS             4291..5934
                     /gene="yojI"
                     /locus_tag="RPM73_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDX6522291.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1447 a         1521 c         1632 g         1448 t
ORIGIN      
        1 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
       61 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
      121 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
      181 ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac
      241 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacaacaaa
      301 gatgtagatg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
      361 gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac
      421 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtgggttct
      481 ttcgactacg gtcgtaacta cggcgttgtt tacgacgtaa cttcctggac cgacgtactg
      541 ccagaattcg gtggcgacac ttacggttct gacaacttca tgcagcagcg tggtaacggt
      601 ttcgcgacct accgtaacac tgacttcttc ggtctggttg acggcctgaa ctttgctgtt
      661 cagtaccagg gcaaaaacgg tagcgtaagc ggcgaaggca tgactaacaa cggtcgtgaa
      721 gcactgcgtc agaacggcga cggcgttggc ggatctatca cttatgatta cgaaggcttc
      781 ggtatcggtg ctgcagtttc cagctccaaa cgtactgatg atcagaatag cccgctgtac
      841 atcggtaacg gcgaccgtgc tgaaacctac actggtggtc tgaaatacga cgctaacaac
      901 atctacctgg ctgctcagta cacccagacc tataacgcaa ctcgcgtagg ttccctgggt
      961 tgggcgaaca aagcacagaa ctttgaagct gttgctcagt accagttcga cttcggtctg
     1021 cgtccgtctg tagcatacct gcagtctaaa ggtaaaaacc tgggtaccat cgctggtcgt
     1081 aactacgacg acgaagatat cctgaaatat gttgatgttg gcgcgaccta ctacttcaac
     1141 aaaaacatgt ccacctacgt tgactacaaa atcaacctgc tggacgacaa ccagttcact
     1201 cgtgacgctg gcatcaacac tgataacatc gtagctctgg gtctggttta ccagttctaa
     1261 tctcgattga tatcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa
     1321 actcattttt ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata
     1381 agctttaccc gcgtggcact gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa
     1441 ccacaacccg ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt
     1501 accttctggc gtgccagcat cccgggcatt gatgccaaac gcagtgccga acttaaagaa
     1561 aagattcaga cccagctgga cgccgacgat cagctgcttt cgacctataa aaaagattcc
     1621 gcgctgatgc gctttaacga ctcgcaaagt ttgtcgccgt ggccagtaag tgaagcgatg
     1681 gccgaaatcg tcactacctc gctgcgcatt ggcgcgaaga ccgatggtgc gatggatatt
     1741 accgtcgggc cgctggtgaa tctgtggggc tttggcccgg aacaacagcc ggttcaaatt
     1801 ccgagccagg aacagatcga tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc
     1861 atcaatcagt cgcatcagca atatctgcaa aaagacctgc cggatttata tatcgatctc
     1921 tccaccgtcg gtgaaggtta tgcggcggat cacctggcac gcttgatgga gcaggaaggt
     1981 atttcacgtt atctggtgtc agttggtggt gctctggata gccgtggtat gaacggtgaa
     2041 ggccagccgt ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggcc
     2101 gtggtggata tcaacggtca tggcattagc acctctggca gctaccgtaa ctattacgaa
     2161 ctggacggca aacgtctttc tcatgttatc gatccgcaaa ccgggcgtcc tatcgaacac
     2221 aatctggtat ccgtgacggt gattgccccg acggcgctgg aagccgatgc ctgggatact
     2281 ggcttaatgg tactcgggcc ggagaaagcc aaagaggttg ttcgccggga agggctggcg
     2341 gtctatatga tcaccaaaga aggtgatagc tttaaaacct ggatgtcgcc gcagtttaaa
     2401 agcttcctta tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtga
     2461 ccgggcagac taaaggctat ccttaaccag ggagctgatt atgaaaaaag ccacatgctt
     2521 aactgacgat caacgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt
     2581 cgttttcgcc gtgcgtacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc
     2641 tttgcgggaa aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ctggctttcg
     2701 cccctgcaaa cgttgtcagc cagacaaagc caatgcccag caacatcggt tggataaaat
     2761 tacccacgcc tgtcgactgc tggaacagga aacgcctgta acgctggaag ccttagccga
     2821 ccatgtggcg atgagtccgt tccatctgca tcggttgttt aaagcgacta ccggaatgac
     2881 gcctaaagcc tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg
     2941 ggagagcgtg acgacgtcta ttcttaacgc cggattcccc gacagcagca gttactatcg
     3001 caaagctgac gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct
     3061 ggcggtgcgt tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga
     3121 gcgggggatt tgcgcgatat tgctgggcga tgatgacgcg acactaatca gcgagttgca
     3181 gcagatgttt cccgctgccg acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg
     3241 tgaagtgatc gccagcctca atcaacgcga tacgccgctg acgttaccgc tggacattcg
     3301 cggcactgct tttcagcaac aagtctggca ggcactgcgc acgatacctt gcggtgaaac
     3361 cgtcagttat cagcaactgg ctaacgccat cggcaaaccg aaagcggtac gggccgttgc
     3421 cagcgcctgt gccgccaaca agctggctat cataataccc tgtcatcggg tggtccgtgg
     3481 tgatggcaca ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg
     3541 cgaagctgaa aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa
     3601 gagccactgg cggctggagc ggtaattttg caccgttttg cttttaacgc tgcggagcaa
     3661 ctgatccgcg atattaatga cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc
     3721 gggggatata ccatgtcggt ggcgatgacc aactgtgggc atctgggctg gacgacccat
     3781 cggcaaggtt atctctattc gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg
     3841 ccacaaagct ttcatgattt atgtcaacgt gcggctacgg cggcgggcta tccggatttc
     3901 cagccagatg cttgtcttat caaccgctac gctcctggcg cgaaactgtc gctgcatcag
     3961 gataaagacg aaccggatct gcgcgcgccg attgtttctg tttctcttgg cttacccgcg
     4021 atttttcaat ttggtggcct gaaacgtaat gatccgctca aacgtttgtt gttggaacat
     4081 ggcgatgtgg tggtatgggg cggtgaatcg cggctgtttt atcacggcat tcaaccgctg
     4141 aaagcggggt ttcatccact caccggcgac tgccgctaca acctgacatt ccgtcaggca
     4201 ggtaaaaaag aataaaaata agaattatta ttgctgtgcg cgcgaagatg tttaaactgc
     4261 gggctgtaat tcattgtccg ggttttctgc atggaacttc ttgtacttgt ctggcggcag
     4321 tatcgctggc catttatcag tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt
     4381 ggcttaattg cttttatcaa tcagcgcctt atcgaaacgg cggataccag tctgctggta
     4441 ttgccggagt ttctgggatt attgctgctg ttgatggcag tcactctcgg atcgcaactg
     4501 gcgctcacca ctttggggca tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg
     4561 attctggata ccaacgtcga gcgcattgaa caactcggta gcgcctcgtt gctggcggga
     4621 ttaaccagcg atgtgcgcaa tatcaccatt gcttttgtgc gtcttccgga actggtgcag
     4681 gggatcattc tcactatcgg ttccgcggcg tatctgtgga tgctgtcggg caaaatgttg
     4741 ctggtaacgg ctatctggat ggcgatcacc atctggggtg gttttgtact ggtggcgcgg
     4801 gtgtacaaac atatggcgac cctgcgtgaa accgaagaca ggctgtatac ggattttcaa
     4861 actgtactcg aagggcgcaa agagctgact ctgaaccggg aacgcgccga gtatgtgttt
     4921 aacaacctct acattcctga tgcgcaagag tatcgccacc atattatccg cgcagacacc
     4981 ttccatctta gcgccgtgaa ctggtcaaac atcatgatgt tgggcgcaat cggcctggtg
     5041 ttctggatgg cgaacagtct cggttgggct gataccaacg ttgccgcgac ctattcgttg
     5101 acgcttttat tcctgcgtac gccgctgctt tcggcggttg gcgcattgcc gacgctgctg
     5161 acggcgcagg tggcgtttaa caagctgaac aaattcgcgc tcgcgccttt caaagcagag
     5221 tttccgcgcc cgcaggcgtt tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt
     5281 gcttatcagg ataacgcgtt ttccgttggt ccgattaatc tcaccatcaa acgtggcgag
     5341 ctgctgtttc tgattggcgg caacggtagc ggaaaatcga cgctggcgat gttgttgacg
     5401 ggcttgtatc agccacaaag cggcgaaatc ttgctggatg gcaaacctgt cagcggcgaa
     5461 caaccggaag attatcgcaa actgttttcg gcagtgttta ccgatgtctg gctgtttgat
     5521 caactgctgg ggccggaggg taaacccgct aacccgcaac tggttgagaa gtggctggcg
     5581 cagctgaaaa tggctcataa gcttgagtta agcaacgggc gtattgttaa cctgaagtta
     5641 tcaaaagggc agaaaaaacg cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt
     5701 atcctgctgg atgaatgggc ggcggatcag gatccacact tccgtcgtga gttttatcag
     5761 gtgttgctgc cgctgatgca ggagatgggt aaaactattt tcgccatcag tcatgatgat
     5821 cattacttta tccacgccga ccgcctgctg gaaatgcgca acgggcaact tagcgagctg
     5881 acgggcgaag agcgcgatgc cgcttcgcgt gatgccgttg cccggacggc ataatattac
     5941 gacgctgacc tggcggactg ctgccgtcag gtcaatatgc taaaaatccc ttcatttcta
     6001 ataaccctat aattaatcaa cgaaattata atgtttctaa aattagaa
//
LOCUS       DAPPNJ010000235         6014 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000235 DAPPNJ010000000
VERSION     DAPPNJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.237"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            218..664
                     /locus_tag="RPM73_003996"
     CDS             218..664
                     /locus_tag="RPM73_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDX6522292.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLRRGDADQVFALLNQTLSS"
     gene            675..2126
                     /locus_tag="RPM73_003997"
     CDS             675..2126
                     /locus_tag="RPM73_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDX6522293.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGGGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2116..3186
                     /locus_tag="RPM73_003998"
     CDS             2116..3186
                     /locus_tag="RPM73_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX6522294.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3186..4934
                     /locus_tag="RPM73_003999"
     CDS             3186..4934
                     /locus_tag="RPM73_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDX6522295.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGEKLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4984..>6014)
                     /locus_tag="RPM73_004000"
     CDS             complement(4984..>6014)
                     /locus_tag="RPM73_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDX6522296.1"
                     /translation="LLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPV
                     AKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPT
                     GEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEM
                     DKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIE
                     EREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADKGGMN
                     FSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEM
                     VDDYNSLR"
BASE COUNT         1414 a         1527 c         1511 g         1562 t
ORIGIN      
        1 ttatatttcc ccgatgcatt gtgagtaaat cacaaaaata atgaataact cattaatgat
       61 tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga cctttcactc
      121 tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc gctccaccat
      181 tacaattgat taaaatatat ttcatggagt ccgattaatg gcagctctta ctgcaagctg
      241 tattgacctg aatattcagg gcaatggcgc ttattccatt ctgaagcagt tggcgacaat
      301 ggcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc tgctgctgcg
      361 cgaaaaaatg cactctactg gatttggttc cggcatcgcc gtgccgcacg gtaaaagcgc
      421 ctgcgttaaa caaccgttcg tgttattcgc ccgcaaagcg caggctattg actggaaagc
      481 cagcgatggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa gcggcgaaga
      541 ggatcaggtc aaaatcatcg gtacactgtg tcgcaaaatt atccaccagg attttattca
      601 ccaactgcga cgaggcgacg ccgaccaggt gtttgccttg ttaaatcaaa ccctcagctc
      661 ataaggaagt ggcgatggag tcatccttac gtattgtcgc gatcaccaac tgccccgccg
      721 ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt tctctcggcc
      781 ataccataaa agtggaaact caagggtcca gtggcgttga aaaccgctta tccagcgaag
      841 agattgccgc tgccgattac gtcattctcg ctaccgggcg tggcctgagc ggcgatgacc
      901 gggcgcggtt tgccgggaag aaggtttatg agattgccat ctcccaggcg ttgaaaaata
      961 tcgaccagat tttcagtgaa ttaccaacaa actcgcagct ttttgccgca gatagcggcg
     1021 tgaagctcgg caaacaggaa gtacaaagcg gcagcgtaat gagccacctg atggcgggcg
     1081 tctctgcggc gctgccgttt gttatcggtg gcggtatcct ggtggcgctt gccaacatgc
     1141 tggtgcagtt cggcctgccg tataccgata tgtcgaaagg cgcgccgtcg tttacctggg
     1201 tggttgaatc catcggttat ctcggcttca cctttatgat ccccatcatg ggcgcttata
     1261 ttgcctcgtc gattgccgat aaacccgctt tcgctccggc gtttctggtt tgctatctgg
     1321 cgaatgacaa agccctgctc ggcacccagt caggtggggg attcctcggc gcggtggtgc
     1381 tggggctggc gattggctat ttcgtcttct ggttccgtaa agtgcgcctt ggcaaagcat
     1441 tacaaccgct gctcggttca atgctgatcc cgttcgttac tctgctggtt ttcggcgtgc
     1501 tcacttacta cgttatcgga ccggtaatgt ccgacctcat gggcgggttg ctgcacttcc
     1561 tgaacaccat tcctccatca atgaaatttg cggcagcgtt tctggtcggt gcgatgctgg
     1621 cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc tcactgctgg
     1681 aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgctgatg ccgcccgtcg
     1741 cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa aaagaggctg
     1801 ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga acctgctatt ccttacgctc
     1861 tggcagctcc gttaccgatg attaccgcca atacgctggc gggcggtatt accggcgtgc
     1921 tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt gacccgctga
     1981 tcggcctgat gtcgccggta ggttcgtttt atctggtgct ggcgattggc ctcgcgctaa
     2041 acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc
     2101 aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtc
     2161 agcggcgatg aacaggaagt tcgcgacatc ctgataaaca cgctggaacc ttgcgttaat
     2221 gaaatcacct ttgatggtct gggcagcttt gttgcccgta aggggaataa aggtccaaaa
     2281 gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat cgacgagagc
     2341 ggttttctgc gcttcaccac cattggcggc tggtggaatc agtcgatgct caaccaccgg
     2401 gtaaccatac gcacacacaa gggatttaaa atccctggtg tgattggttc cgtcgcgcct
     2461 catgcgttaa cggaaaagca aaagcaacaa ccgctgtcat ttgatgagat gttcattgat
     2521 attggcgcga acagtcgcga agaagcggaa aagcgcggcg ttgaaattgg cgattttatt
     2581 agcccggaag ccaattttgc ctgctggggc gaagataaag tagtcggaaa ggcgctggat
     2641 aaccgcatcg gctgcgcgat gatggcggag ctactacaga cagtaaataa cccagaaatt
     2701 acgctgtacg gcgttggcag tgtggaagaa gaagttgggc tacgcggggc acaaacctcg
     2761 gcggaacaca ttaaaccgga tgtggtgatt gtgctggata ccgctgtcgc aggtgatgtt
     2821 ccgggcattg ataacattaa atacccgctg aaactgggcc aggggccggg gctgatgctg
     2881 tttgacaagc gctacttccc caaccagaaa ctggtagcag tgttaaaaaa ctgtgccgca
     2941 cataacgatt taccgctgca attttccacc atgaaaaccg gagcgacgga tggcgggcgc
     3001 tacaacgtga tgggcggcgg gcgtccggtt gtcgcgctgt gtctgccaac ccgttatctg
     3061 cacgctaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac gctgatacgg
     3121 gattttctga cgaccttaac tgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg
     3181 gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa cagccgcgca
     3241 cgcctggaga gctggcgcaa cagactggcg tttcaggcag gaccatcctg cgtgatattg
     3301 actatctcaa cttcaccctt aacggcaaag cccgcatttt cgccagtggt agtgcgggct
     3361 atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag cacgataacg
     3421 acgatcggct gctggcgcta ttattactaa atacctttac tccccgtgcg caactcgcct
     3481 cggcgcttaa tttgccagaa acgtgggtag cagaacgtct gccccggtta aaacagcgtt
     3541 atgaacgcac ttgttgcctg gccagtcgcc ccggtttggg ccatttcatt gatgagacag
     3601 aagagaaacg cgttatcttg ctggcgaact tgctgcgcaa agatccgttt ttaattccgc
     3661 tggcgggcat aacacgagac aaccttcagc atttatccac ggcctgcgac aaccaacacc
     3721 gctggccgct catgcagggt gattatctct ccagcctgat tctggcgatt tacgccctgc
     3781 gtaatcaact gaccgatgag tggccgcaat atcccggtga cgagataaaa caaatcgttg
     3841 aacatagcgg tctgtttctt ggttataacg ctgtaagaac cctgacgggt ttgatagaga
     3901 aacagcatca gcaagcgcag gtaatttcag ccgatcatgt gctggggttg ctgcaaaggg
     3961 tgccgggcat cgcgtcattg aatattattg atacgcagct ggttgagaac attaccgggc
     4021 atttattacg ttgcctcgcc gcaccagtgt ggattgctga gcaccgccaa agcagcatga
     4081 ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt attacgctac
     4141 tgcgtgaaca gctcgatatt ccccttttcg acagcgatct gatcggtttg tattttgcct
     4201 gtgcgctgga acggcatcaa aacgaacgcc agccgatcat tttgctctcg gaccagaacg
     4261 cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt cgggtgatta
     4321 ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga gattgagccg ttattgatca
     4381 ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc gtaaaaaata
     4441 tcattacggc tgccggtatc gaacaaataa aacattttct ggcgacggca tttattcgcc
     4501 aacagccgga gcgttttttc tctgcccccg gaagttttca ttattcgaat gtacgcggtg
     4561 aaagctggca acatattacc cggcaaattt gtgcgcaatt agtggcacaa caccatatta
     4621 ccgccgatga agcacaacgc atcatcgccc gcgaaggcga aggtgaaaaa ctgattgtta
     4681 atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt ggatttttta
     4741 ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc ttgatcgcct
     4801 gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca agcgtattgt
     4861 gtcagcatcc ggcagaggtt attgccgggt taacaggata tgaggcattt atggagttac
     4921 ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcattta tcatcaaaaa
     4981 cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta atgctctcgg
     5041 gaaagagtcg tcgaccatcc agcttgagtt tgcgtcctgc agttgctctt tatcccagtc
     5101 agagtacggg actttatctc tgacgcggcg aacgtatttt ttagcctcga gttgatactg
     5161 gcctgccgaa ttaataaacg agaaattcat gccgccttta tccgcttctt tggcctgcgc
     5221 caccagcgtt tccagttcag acactttctt cattgccgct tctgcatcaa acttatcttg
     5281 cgatatcagg ttattaattt gtttcgctga aatcatgact gccagagagt aatagtggaa
     5341 tgttttccct tctctttctt caatattttt cagttccgca agctggcgct tatcgttaat
     5401 ttcctgaatc gccgcatgat aagattctgc tacagcttca aaggcttcca gattttttaa
     5461 gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat tttcctgggt
     5521 gtaatattta tccatttcgt taatggtatt acccaacgcc acggcagcat cgatataact
     5581 caccgccacg ccatcaatag gttgcagcgc aggcgttaac gccacagcac tttttacgcc
     5641 tttttcacac tcagcgaggt tggattcact aatgccgtag atgccataaa cagtacgctc
     5701 ttcaccggta ggcccctgtt taaaatcttt cagccagtca gcataacgcg ccagactgct
     5761 ctgtacaggg atctgtaact tgttatagca cttgatatag acatccattt tttcgtcgaa
     5821 aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca ggtttagctt ctgcttttgc
     5881 aacgggagct tcaggagctg gtgcggcagg ttgactattg gcaggcgggg gcgtttctgt
     5941 ttcggctttt ttatcatcac aacccgtcag accgagggcc atgatggcga ctataatagc
     6001 ggaagataat aaat
//
LOCUS       DAPPNJ010000236         5915 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000236 DAPPNJ010000000
VERSION     DAPPNJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.238"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="RPM73_004001"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="RPM73_004001"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDX6522297.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="RPM73_004002"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="RPM73_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDX6522298.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="RPM73_004003"
     CDS             complement(1535..1801)
                     /locus_tag="RPM73_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDX6522299.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="RPM73_004004"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="RPM73_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDX6522300.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1490 a         1506 c         1509 g         1410 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tatatgagaa cgaacgcgat ctattctttg aagataaatc caacgacgta
      841 attcaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctatt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcattccagg ggagctgatt cagataatcc ccaatgacct ttcatcctct attcttaaaa
     1081 tagccctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgctcgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgta acgccagaac
     1201 agttacagca tcatcgtttt gtgctggaaa gcgtaaacgg taagcccgtg accaacgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcactacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tcttttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcgacgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca cgattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaatccg
     2941 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag aagcctttaa
     3121 taatggtgag cgcgaaaccc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtgactaat ctgtcactgc cgttgccgga
     3301 aaacaccctg ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa agcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaagaagt
     3661 tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacacaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggtcagg atctggtgga acgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcagcc ggttaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtct cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctgt ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacagcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     4861 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tcaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca tcgcttatag
     5281 cccatgtgaa gagcatggtt acgatctggc actcagccac gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatccgcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agtagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcggctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
     5761 attaaaagta taatttttat tttgtatgta ttatcttcga tacattcacc agacttatta
     5821 tcatttcaga ggaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc
     5881 ggctaactga acttctcctt taggatgttt tcact
//
LOCUS       DAPPNJ010000237         5807 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000237 DAPPNJ010000000
VERSION     DAPPNJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.239"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(55..441)
                     /gene="ridA"
                     /locus_tag="RPM73_004005"
     CDS             complement(55..441)
                     /gene="ridA"
                     /locus_tag="RPM73_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDX6522301.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(514..975)
                     /gene="pyrI"
                     /locus_tag="RPM73_004006"
     CDS             complement(514..975)
                     /gene="pyrI"
                     /locus_tag="RPM73_004006"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDX6522302.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(988..1923)
                     /gene="pyrB"
                     /locus_tag="RPM73_004007"
     CDS             complement(988..1923)
                     /gene="pyrB"
                     /locus_tag="RPM73_004007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDX6522303.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1927..2061)
                     /gene="pyrL"
                     /locus_tag="RPM73_004008"
     CDS             complement(1927..2061)
                     /gene="pyrL"
                     /locus_tag="RPM73_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDX6522304.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2342..2737)
                     /locus_tag="RPM73_004009"
     CDS             complement(2342..2737)
                     /locus_tag="RPM73_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDX6522305.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRQAFNNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(2868..3581)
                     /gene="bdcA"
                     /locus_tag="RPM73_004010"
     CDS             complement(2868..3581)
                     /gene="bdcA"
                     /locus_tag="RPM73_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6522306.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHEQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3652..4245
                     /locus_tag="RPM73_004011"
     CDS             3652..4245
                     /locus_tag="RPM73_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDX6522307.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAKCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILEE"
     gene            4390..4842
                     /gene="tabA"
                     /locus_tag="RPM73_004012"
     CDS             4390..4842
                     /gene="tabA"
                     /locus_tag="RPM73_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDX6522308.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4965..5633
                     /locus_tag="RPM73_004013"
                     /pseudo
     CDS             4965..5633
                     /locus_tag="RPM73_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1522 a         1328 c         1425 g         1532 t
ORIGIN      
        1 ttgcgtttgt catcagattt aatccgggca tgatagcccg gatttccatc aagattagcg
       61 acgaacagcg atcgcttcga tctcaatttt tacgtctttc ggcagacggg caacttcaac
      121 gcaagaacgt gccgggaagg tggcgttgtg ttcggtgaag aaggcttcgt aggtagcatt
      181 aacggttgcg aagtcgttca gatctttcac aaacacggtg gttttaacga tgtcgcccac
      241 tttcaggcca gcggcttcga cgatcgcttt tacgttatcc agcgactgac gtgcctgtgc
      301 agcgacgtct gccggtactt cacccgtttt cggatttacc gggatctgac cggaggtgat
      361 gatcatattg cccagatcaa cgccctgtac gtaagggccg atagctgccg gtgcattttc
      421 cgtcgcgata gttttgctca tgatttctcc tttattacag cggtaaaaag tctggctcat
      481 tatagggagc cagactttta ttaccaaccg caattaattg gccagcacca cattatggga
      541 aaactctttt tcacagtatt tgcatttgag cgcgatgtca ttggcgcgtt ttcgcacggc
      601 aaagctggaa gaaaccggtt cggcatggct gatacagttg ctgttcgggc agaccagcac
      661 attgtcgatg cgctccggca gactggggcg cgatttaccc accacttcat agttgtcgat
      721 acggttaacc gtggcttgcg gcgcatacaa tgccagttga tctacctgat cttcactcaa
      781 aaaggtattt tcgattttga tcagatcttt gcggcccatc tcgccagaag gcaagttcag
      841 accgatggtg atgcgctgat ccgtttcggt cagcttgaac agactcaaca gcttaaaacc
      901 gatctgggcg gggatatggt caattaccgt gccgcgttta atagcttcaa cctgcaattt
      961 attatcgtgt gtcatctcta tttcccctta cagtaccaga tcgcgattca gaaccagtgc
     1021 cagtaacgcc tggcgagcga atatcccatt gcctgcctgc tggaagtacc aggcgtgtgg
     1081 cgttttatcc acatcggtgg caatctcatc aacacgcggc agcggatgca gcactttcat
     1141 attggctttg gcgttgtgga gatcgctggc gcgaagaaca aactgcgctt ttacgttggc
     1201 gtattcggat gggtccaggc gctctttttg cacgcgggtc atatacagga tgtctacttc
     1261 cgccatcact tcttcaatag agctgtgcag actccaggcg atcccttttt catcaagcat
     1321 atccagaatg tattgcggca ttgccagcgc gtccggcgcg atgaagtaaa aacggttgcc
     1381 gtcgaacttc gctaacgcct gagtcaggga gtgaacggtg cggccatatt tcaggtcacc
     1441 aaccattgcg acgtggagat tgtccagacg cccctgggtt tcctgaatgg tgaataagtc
     1501 cagcaaggtt tgcgtcggat gttggttgga gccatcaccg gcattcagta ccggtacatt
     1561 gccggaaaac tcggtggcca ggcgcgccgc accttcctgc ggatgacgca tcactatcgc
     1621 atcgacgtaa gtgctgataa ccgaaatggt atcggccagc gtttcgccct ttttacccag
     1681 tgatgtattg gcgctgtcgg aaaagcccac cacgctggca cccaggcggt gcatggaagt
     1741 ttcgaaagag aggcgggtac gggtagaggc ttcgaagaaa cagctggcaa tgactttgtg
     1801 cttcaacagc tctggttgcg ggtttgcttt cagtttcgcc gctgtcgcca gcaccagatt
     1861 aaggtcatcg cgactaaggt cgtttatgga aatgatatgt ttctgatata gcggattagc
     1921 catcttttat ctcctgacgc ctgggcaaaa aaaagcccct cgattgaggg gctgggaatg
     1981 ggtgatcaac gggaagaaaa acggcaggcc agcgtctttt ttcagacgcg gtaagacaaa
     2041 atgtcgaaca cactgaacca tacatcctcc cggcaaattg tccggcatta tactcatcgt
     2101 cagaagcggc gcaagcattt gatgcaatat tttgtcagcg caaacggttt atttgaatta
     2161 aaagtcaagg tatatgcatt tttattcgat gtgattctgg aggggaactg tccttcggta
     2221 tcaataattg cagacatttc ctgctttttt ctttttcact ttcacgcaat cagatatgca
     2281 ttttattcca ttcatccgac ttatagggcg gagtgtgaaa acgaacggct aacactattg
     2341 cttactgctc agggatgcgc gctatcactt taatttcaaa atcaaagcct gccagccatg
     2401 taacacctac cgccgtccag tttggataag gtggggcgct aaatatttca tttttcaccg
     2461 tcatgatgtc ttcaaattgg ttttctggat cggtatggaa gctcgtaaca tcaatgatat
     2521 cgtcaaaagt gcatcccgca gcagccaggg tcgcatgcaa attattaaat gcctgtctga
     2581 cttgttgctg aaaatcgggt tctggtgttc catcctctcg acttcctact tgcccggaaa
     2641 caaacagcaa atcgccggaa cgaatagccg cagaataacg atgctcagca tacagtgaat
     2701 gtcggccagc agggaaaaca gcggttcttt ctaccatttg gttatcctca agatttacga
     2761 catgaacaga agagttctct ttaccgggag ccgcttttag cggacgacgt gagtaaacaa
     2821 aacccagaca tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac
     2881 gcgccatcaa tggtatgcat cgcgccggta acaaagctgg cttctggccc tgctaaccat
     2941 gcgaccatac cagcgacctc ttccggttgc tcatgtcttt tgatagccat aaaaccatgc
     3001 aacatatcgc gcattggccc gttggcggga ttagcgtcgg tatcaattgg ccctggctgg
     3061 acgacgttga tggtgatccc acgcggtcca aaatcacggg ccagcccgcg cgccatgcct
     3121 tgcagggcag atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca
     3181 ttcacggagc cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaacg
     3241 gaggcatggt aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt
     3301 aattccaggg catcgccaaa gacgccaata cctgcattaa ccaccaggat atccaatgcg
     3361 ccgctcttac gaacgacatc aatgacagcg tctctgtcag cactatctgt gaatactgct
     3421 gtcgctccag tctcttgtgc caggcgttca gcggcatctt tcgaccccgc ataggtgaat
     3481 cgtacattgg ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacggctg
     3541 ccaccgagga tgagaactgt cttacctgta aaagcgccca taaggactcc ttgatttatt
     3601 atgtaacatg cattacaaaa ctgttttaac tttctgtcaa caagttttgt aatggtcact
     3661 aaaaaacaat ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct
     3721 gcggcaaaag tgctttttca ccaaaaaggt ttcgatgctg tcagtgttgc tgaagttact
     3781 gattatcttg gtattaaccc cccgagcctc tacgcggctt ttggcagtaa ggctgggtta
     3841 tttagccgtg tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt
     3901 cgtgatgatc gtccggtagg cgagtgtctg gttgaggtat taaaagaagc ggcgcgcaga
     3961 tatagccaaa acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat
     4021 ccacaagcgc gtgatattgc cgttcaatat tatcacgccg cagaaacgac catttatgac
     4081 tatatcgcca ggcggcatcc acaaagcgca aaatgtgtga ctgattttat gagtaccgtg
     4141 atgtcagggc tttctgcgaa ggcacgggag gggcactccc tcgaacaact ctgtgcaaca
     4201 gctgcactgg cgggggaagc gataaaaact attctcgagg agtgatgctg accttgaccc
     4261 gaatggcggg aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc
     4321 tagcggaatt gatcatcttt tcgtataacg atagaaatga aacgttgttt taattaagga
     4381 gtggaaaaga tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta
     4441 cgccaggcga ttgagcatat caaagcgcac gttacgccag aaacgccaaa gggtaagcac
     4501 gatatcgaag gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa
     4561 gctcgccgtg cggagtacca tgcccgctat ctcgacattc agattgtgtt aaaaggtcag
     4621 gaaggcatga ccttcagcac gcaacctgca ggcacgccgg agaccgactg gttagctgat
     4681 aaagacatcg catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt
     4741 gattttgttg tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggtgcacca
     4801 gcccaggttc gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat
     4861 aactccaggt ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa
     4921 aatttgtaat gcttagacgt tcttattaac tcaaggagtt cgtcatgagc aaaatatcag
     4981 atttcaatta ttctcaacac attacattag ccgacaattt taaacaaaaa agtgaagttt
     5041 taaatacctg gcgtgttgga atgaataatt ttgctcgtaa tgccgagggg caggataaca
     5101 caagaaatat cctcgatcct aagacatttt tggagttttt ggtaaaaata tttaccctgg
     5161 gttatgtgga ttttagcaaa cgctccaacg aagcgggaag aaatatgatg gctcatattg
     5221 agtcctcatc ttatatcaaa aataatgatg gcagtgagat aatgaagttt gttatgaata
     5281 atcctgaagg ggagcgagcg gatttatcta aggtggaaat agaaattaca ctttccacta
     5341 tcactactat ggggactcgt caaggacata cggctattat atttccacaa cctgatggtt
     5401 cgactaaccg ttatgaaggg aagtcctttg aaagaaaaga tgagagttca ttacatctga
     5461 ttactaacaa ggttctggcg tgttaccaac gcgaagctaa caagaaaatg gcgcttctat
     5521 taaataatca tcagttgaac tggaaggacg caattgatta taacgagtta ttagaaaagc
     5581 cattagccag tacattagaa aaaataaaga aagaacatct gctattaatg cctcatgtat
     5641 gtgatgacac gattccatac ttactaggcg aaggtggtat acttgaagaa ataaatgatc
     5701 tgaaaacgtt acatgatacc agaattgcta gtggtacaga aggtaatgat gaaattaata
     5761 acatcaaaat taacctgtct aatattctta ttgattcctt agatgat
//
LOCUS       DAPPNJ010000238         5799 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000238 DAPPNJ010000000
VERSION     DAPPNJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.240"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(310..1269)
                     /gene="nrdF"
                     /locus_tag="RPM73_004014"
     CDS             complement(310..1269)
                     /gene="nrdF"
                     /locus_tag="RPM73_004014"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDX6522309.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1279..3423)
                     /gene="nrdE"
                     /locus_tag="RPM73_004015"
     CDS             complement(1279..3423)
                     /gene="nrdE"
                     /locus_tag="RPM73_004015"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDX6522310.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3396..3806)
                     /gene="nrdI"
                     /locus_tag="RPM73_004016"
     CDS             complement(3396..3806)
                     /gene="nrdI"
                     /locus_tag="RPM73_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDX6522311.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3803..4048)
                     /gene="nrdH"
                     /locus_tag="RPM73_004017"
     CDS             complement(3803..4048)
                     /gene="nrdH"
                     /locus_tag="RPM73_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDX6522312.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4296..4625)
                     /locus_tag="RPM73_004018"
     CDS             complement(4296..4625)
                     /locus_tag="RPM73_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDX6522313.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4777..5121
                     /locus_tag="RPM73_004019"
     CDS             4777..5121
                     /locus_tag="RPM73_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDX6522314.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5158..5607)
                     /gene="alaE"
                     /locus_tag="RPM73_004020"
     CDS             complement(5158..5607)
                     /gene="alaE"
                     /locus_tag="RPM73_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDX6522315.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1483 a         1420 c         1438 g         1458 t
ORIGIN      
        1 atggaaatag tcactttttt ctaccctaac atactgagaa tctgaggcaa cccctgaggg
       61 caaatgtggc atgaatattg atgcgagcaa atcgccggaa tttcccgtga tataagggct
      121 gagagcaaat cgagaaaagt tgatattttt tgccatagat agcgaaaaaa cgcctgaata
      181 ttgtagtttc aggcgaataa tgcaacagag tatcagtgta gatcactaca gaatatttgc
      241 gtgataaaaa gctatttggc gggaattatt tccctgctgc gggtagtgat atttttgaaa
      301 ataacaccct cagaaattcc agtcttcatc ttctgtttca accgctttcc ccatcacata
      361 agaggagcct gaaccggaaa agaaatcgtg attttcatcg gcattcggcg aaagcgcggc
      421 gaggattgcc ggattcactt ccgccatttc tgcgggaaat aacggttcgt agcccagatt
      481 catcaaagcc ttattggcgt tgtaacagag aaacgctttc acatcgtcag cccacggggt
      541 ttcggcgtac agctcatcgg tgtattgcaa ctcgttgtcg tagagttcca gcaacaaatc
      601 gaaggcgaaa ctcttcaact cttcacgttg tcccagagat atcttttcca tgtttttctg
      661 atatttatag cctatgtagt aaccgtggac tgcttcatcg cggataatca gacggatcag
      721 atctgcggta ttggtcagct ttccgcggct ggaaaaatac atcggcagcc agaaaccgga
      781 atagaacaaa aaagattcaa gaaacacact ggcgattttc tttttcagcg gatcatcacc
      841 gcgataatgt tgctgaataa tctgagcttt tcgctgcaac ggtgcgtttt cttcactcca
      901 ggcgtaggcg gcatcgacat ctttggtctg acacagcgtc gagaaaatcg aactgtaaga
      961 gcgggcatga accgcttcca taaagctgat attcgataat accgcttctt catgaggcgt
     1021 gagtgcatcg ggcatcagcg aaggcgcgcc gataacattt tgcagcgtgt cgagcagcgt
     1081 caggccagta aaaacgcgca tcgtcagttg ttgttctacg acagttaatg tctgccaggc
     1141 aggaatatcg ttcgacagcg gcaccttttc tggtagccag aaattgctgg tcaggcgatt
     1201 ccacacctcc agatctttat cgtcagatat cttgttccag ttgatggcgc tgatacgtga
     1261 gagtttcata gatattcctt aaagtgcaca ggagacgcag ccttcaattt cagtgccttc
     1321 cagcgccatc tgacgcaggc gaatgtaata gagcgttttg atacctttgc gccaggcgta
     1381 aatctgcgct ttgttgatat cgcgagtggt ggcggtatcg gggaaaaaca gcgtcagcga
     1441 caaaccctga tcgacatggc gagtcgcttc cgcgtaggtg tcgatgatct tttctgcgcc
     1501 gatttcgtaa gcatcctgat acagcgccag attctcgtta gtcataaacg gggcagggta
     1561 gtaaacgcgt cctgttttgc cctctttgcg tatctctact ttcgccacaa tcggatgaat
     1621 actcgacgta gcatggttga tataagagat agaaccggtt ggcggcaccg cctgaagatt
     1681 ctggttataa atgccgtagc gcatcacgtc gtcgcgcagt tgcgcccaca tctcacgggt
     1741 aggtaacgta ataccgctac gggcaaacag ttcgccaact ttcgtcgttt tcggctgcca
     1801 gttcccttgc agatattggc tgaaatattc actactggca tagcgtgact gtttgaaccc
     1861 ggcgaaggtt tcaccgcgtt cgcgtgccag caacatcgag gtacgcagtg cgtgccaggt
     1921 gatggtatag aaatagagat tggtgaaatc cagtgcttcc ggcgaaccat aagcgatgcc
     1981 ttctcgcgcc agataaccgt gtaaattcat ctgccccagc ccgatggcgt gtgaggcggc
     2041 atttccggct tcgatggacg gcacgctgcg gatatgactc atatctgaca ccgccgttaa
     2101 accgcgtacg gcagtctcta ccgtgcgggc aaagttgggg gaatccatgg tgtgggcaat
     2161 attcaacgaa cctaaattgc aggaaatatc atggcctgta cgggcatagt cgagattctc
     2221 gtcatactct gaggcgctgt taacctgcaa aatttctgag catagattac tcatatttat
     2281 gcgcccggcg atagggttag cacggtttac cgtgtcttca tacatgatgt agggatagcc
     2341 ggactcaaac tggatttctg ccagtcgctg gaagaaatca cgggcgttga ggtatttttt
     2401 gcggatgcgt tcatcggcaa ccagttcgtc atagtgttcg ctaatggcga tatcggcaaa
     2461 cggcttgcca taaactcgct caacgtcata gggcgaaaac agcgccatct gcgcattctc
     2521 tttggccaga tggaaagtga tatccgggat caccacgcca agcgacagtg ttttaatgcg
     2581 gattttttcg tcggcatttt cccgtttcgt gtcgagaaaa cgcagaatat cgggatgatg
     2641 agcatgtaaa tagactgcac cagccccctg acgcgcgccg agttggttgg cataggaaaa
     2701 tgcgtcttcc agcattttca tcaccggaat tacgccagaa gattgatttt caatacgttt
     2761 aattggcgcg cccgcttccc gcagattcga cagcaaaaat gctacgccgc cgccgcgttt
     2821 cgacagttgc agtgcggaat ttaccgcccg accaatcgac tccatattgt cttcaatacg
     2881 cagcaaaaaa caggaaacca gttcgccgcg ctgctgctta ccgcagttga ggaatgtcgg
     2941 cgtggctggc tgaaagcgtc ctgacagcat ttcatcggtc agttgcaacg ccagcgtcgc
     3001 atcgccttgt gccagcgtca gcgccaccat cgtgactcga tcggcaaaat cttccagata
     3061 acgtttaccg tcgaatgtct tcaacgtata gctggtgtaa aacttccatg ccccgaggaa
     3121 tgtctggaaa cgaaaaccgc tggtgtgtgc gtgggcaaac agcgtaatga caaagtcgcg
     3181 agagtagcga ttaagaacgc tttcatcata gtaaccttcg ttgaccagcc agttcaggcg
     3241 ctgctgctgg ctactgaagg tcacactgtt cggacgcaca tgcgtcgcca taaaggcgtc
     3301 aacggcctgg cggtctttat cgaactgaat gcgacctgcg ctatcgtaga ggttaagcat
     3361 cgcattcagc gcgtggtaat ccatcgtttc ctgcgtcagg cattctgcgg ttgtcgttgc
     3421 caaaattcgg ttactccttt acgaacgttt tcgatatcgc tttgcgtacc catgagttca
     3481 aaacggtaca gccacggcac gccgcatttc cgggcaatca catctccggc gcggccatac
     3541 gcctcaccaa agttgcgatt acccgaagca ataacgccgc gaagcaacgc ccggttgtgc
     3601 tcgtcgttta aaaagcgaat tacctgtcgt ggcaccgcgc cagccgtacc gccgccgccg
     3661 taagagggca cgatcaggat gtaaggctcg tctacctgaa tccgttcccg ctcattgagc
     3721 gggatgcgca ccgcgggcag tcctaaacgt tcgataaaac gctgcgtatt ttcggagcta
     3781 ctggaaaagt agacgagctg gctcatgcac tggccgcgtg tggcgctgga tgcagacgat
     3841 tgatcatgtc cgggcggaaa ccagaccagc taagatcgcc agcaatcact accggcaact
     3901 gacgaaagcc ctgagcacgc agcgcttccg ccgcttcagg aacgcgatcg acattaatca
     3961 tttcaaaatc aaagccccgg ttttccatcg cccgtttggt ggcgtggcac tgaacgcaat
     4021 cgttacgagt gtaaatagta atgcgcatga ttcgtatttc cgtttaaaat gaagatacgg
     4081 cgcgatgata cgcgtcgggt tgtctctctg ttgatacaga gatactagat gtagttgaaa
     4141 aaagattcaa ccacacaata tatagcaaat agtggtcgaa attaccctgg atatgagcgt
     4201 gacggggtag ggggattttt gtgattcacc aggcaaaaag aaaccccgaa gacaggcttc
     4261 ggggtcaaag acgcgtattt attatcattt ttgcactacg atttgcgcat gcttaacagt
     4321 gcgccgataa aaatacctac cgcagctgct gtacccacgc tacaccaggg tctttcacga
     4381 acaaaagaat ccgcgcagcc aacggcatcg cgtgcggctt gctggacgcg agtacgacca
     4441 tgcattcgcg ccctggtttc tttcagcaat gcctgagctt tactgcgtgc ggcttcagct
     4501 tcccctttgg cgtcgctgcc ccaggacttt aatacagatt cgaggctgtc agctaattga
     4561 ttgacatcat tctggatatc ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta
     4621 aacatatgat cccccattag aattcgatgt aattttcagt gtagaccata attttcttaa
     4681 ctgaggttca tcgcggcttt atggactttt ctgaaagcgt tgtgaatgcg caatcaggta
     4741 aggtaggccc gcggttgata ataacgagga tcaaatatgt atttacgacc agacgaggtg
     4801 gcgcgcgtac ttgaaaaagt cggttttact gtcgatgtgg taacccagaa agcgtatggt
     4861 taccgccgtg gggaaaatta tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgct
     4921 ctggtaattc atccaacatt aaaagaacgc agttcaacac tggctgaacc cgcttccgat
     4981 attaaaactt gcgatcatta ccagcaattt ccgctctatt tagcaggcga gcgacacgag
     5041 cattacggta tcccgcatgg cttcagttcg cgtgttgcgc ttgaacgtta tttgaatggt
     5101 ttatttggcg aagccagtta aagacgcgac tggcgatgcc agtcgcgaaa agaagagtca
     5161 ggcttttacc tgctggtaac ggctgacttt aaacagtcgg cggcaataat cgaggaagta
     5221 gccataaacc gcccccatca acatcgaaac aacgatgttt gaactgaccg ccgccataat
     5281 ctgatgccag tctgcgccca ccactaacaa gatcgccaca tacaccggtg actggaacgt
     5341 cacataagcc aggatatcag ccagattttt tatccagccc gacgggctaa ctttgcgtgc
     5401 cgcgcgcata aacagatcgc ggtacatacc gtatggccat gcaattaaga tgttcaccgg
     5461 aatcgctacc aatctggaat aaaaagactg ttcgaagctc attccggaga ggaaaacttc
     5521 aatacacatg ttcacgacag aacagtaaac aaccatcgcg aacgtatctg caactgcatg
     5581 acgcaagcgt gactgcggtg agaacatgct aatgctcctt aataaaagcg taatgggatg
     5641 ttaatggaga tgaaagcagt ggatctcatt gtcatctgca ttttcgcata gcgtatatcg
     5701 ctggctatga ctattcaatt agtgaaaaat ttttatttag tcagtttttg tagataaaat
     5761 attccgtaac ggctcgtttt ttgtacattg tggattttt
//
LOCUS       DAPPNJ010000239         5749 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000239 DAPPNJ010000000
VERSION     DAPPNJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.241"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(270..491)
                     /locus_tag="RPM73_004021"
     CDS             complement(270..491)
                     /locus_tag="RPM73_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522316.1"
                     /translation="MHFRKMKVVFLFIHILAVVMISVMTVHLCSMAVEKVPEECDIPV
                     RVAALVVLWLVFSGLIYWLFTAFMKTDVM"
     gene            complement(506..697)
                     /locus_tag="RPM73_004022"
     CDS             complement(506..697)
                     /locus_tag="RPM73_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522317.1"
                     /translation="MSDFVRHFSVKDKKEDVVMQDMHFLLEDFICAHISRKSGRVPGR
                     LRQRMDKLRLQRIRKDRTG"
     gene            complement(810..1844)
                     /gene="f17g"
                     /locus_tag="RPM73_004023"
     CDS             complement(810..1844)
                     /gene="f17g"
                     /locus_tag="RPM73_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17G fimbrial adhesin"
                     /protein_id="HDX6522318.1"
                     /translation="MTNFYKVFLAVFILVCCNISQAAVSFIGSTENDVGPSPGSYSRT
                     HAMDNLPFVYNTGNNIGYQNANVWRISKGFCVGLDGKVDLPVVGSLDGQSIYGLTEEV
                     GLLIWMGDTNYSRGTAMSGNSWENVFSGWCVGANTASTQGLSVRVTPVILKRNSSARY
                     SVQKTSIGSIRMRPYNGSSAGSVQTTVNFSLNPFTLNDTVTSCRLLTPSAVNVSLAAI
                     SAGQLPSSGDEVVAGTTSLKLQCDAGVTVWATLTDATTPSNRSDILTLTGASTATGVG
                     LRIYKNTDSTPLKFGPDSPVKGNENQWQLSTGTETSPSVRLYVKYVNTGEGINPGTVN
                     GISTFTFSYQ"
     gene            complement(1846..4314)
                     /locus_tag="RPM73_004024"
     CDS             complement(1846..4314)
                     /locus_tag="RPM73_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6522319.1"
                     /translation="MLSVPLWAAEDAEFDPSFLSRTPGSSVIDVRRFTHGNPVPSGNY
                     YSDIYLNGEWKGNAELSFRDMSSSQEESVLCVTPELLSILDVSPEAVAREPDTGNGRC
                     MPFSAVVPDAKIRFDLSLLRLNVEIPQAMLVSRPRGYISPSQWQTGVPAAFVSYDVNH
                     WRYRTPDMENNQSYLGLRAGFNAGGWAFRHRGTESWSDGHAEGYRSIETNLQHDIAML
                     RAQLTLGDFTTTGELMDSNSLRGIRLASDDRMLPGSLRGYAPVVRGMAGSNARVTVRQ
                     NGNIIYETTVPAGPFSISDLYPSGYGGDLTVTVTESDGQTRSFIVPFASVAQLVRPGF
                     SRWQVAAGRYRYGNRTFSDTVFQGTLQYGLTNDITLNTGMSTAPHYLSGLGGVAFNTP
                     LGAVATDITLARTTFPGSDTTHRGYSLHAGYSARIPETSTNVTLAAYRYSSRDFWSLR
                     DAILTRNRDVINDSSVRSATGYRPRNQLQLTVNQSLGEGWGNLYLTGATYHYWGHTGT
                     FNEYQAGYNNVWKKMSYQIGFSQTRRSGDYHHDNRFFFSFSLPLSDTGRTLLSTTVNV
                     SDKNSNSIQSSISGVSGDDNQLSYGVSLNTQENGPTGYAVNGSYRSPVATLQATAGND
                     SRHNRQISAGISGAIVAHPFGVTASNDLGDTFTVIHADGAGGAVINNAPGNRLDPWGN
                     GIVPYVTPYEKNYISIDPTALAADVELSATEREIIPRANSTTMVTFTTKTGKAMLFDV
                     KMPDGTPPPMAAEALTADGKSAGYIAQGGRLFVRDLDAEKGMLRIVWGSGQNDSCVIH
                     YSASGENARGNAVPENHHVVCMRQ"
     gene            complement(4397..5119)
                     /locus_tag="RPM73_004025"
     CDS             complement(4397..5119)
                     /locus_tag="RPM73_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX6522320.1"
                     /translation="MKHILLFIFTVLLSLPSYGSVVIMGTRVIYPAEQKSINVRLNNG
                     DSTPSLIQAWLDTGDPSSPPDSVRVPFIITPPVFRMEPLSGQTMRIMYTGEKLPADRE
                     SLFWLNVLDIPAKPSFAGKSEKAQGYNYLQFAVRSRIKFFFRPDGLPFSPDDAYKKVT
                     WQLTGGDTLQASNPTPYFITFSTVSVGQGAQKVSSTQGGMAAPFSSVTFRLKQKVSPS
                     GKVEWTVVNDNGGYQKGESVLK"
     gene            complement(5187..5732)
                     /gene="f17a"
                     /locus_tag="RPM73_004026"
     CDS             complement(5187..5732)
                     /gene="f17a"
                     /locus_tag="RPM73_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021578761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17A fimbrial adhesin"
                     /protein_id="HDX6522321.1"
                     /translation="MRKIQFILGILAAASSSATLAYDGTITFNGKVVDQTCSVTTESK
                     NLTVTLPTVSSNSLTSNGEVAGLTPFTISLEGCTSPASAGAENVKAYFEPNATTDYDS
                     HNLNIASGSGNATNVQIQLLNADGTTPILLGTDSAGQAVTPVAINGTNVTLRYNARYY
                     ATGKATAGNVSATVNYTIAYQ"
BASE COUNT         1537 a         1524 c         1256 g         1432 t
ORIGIN      
        1 gtctctgtga atatatacag aaaacatatc tcataaaacc cgggaaatgc cccatgaata
       61 caggacagtg aaaacaggca tgataatcag gaaagccatg cctgatacag aaaaagttac
      121 ctgcggggat aatgcatgcg tattttcatg gaaggagatc actcaataac ttccatcgag
      181 agcgggtaat aactcaccga tctccttcac ggtaataagc aatgcacaac caggcactta
      241 ctggccggtt atccatgtct atattatatt tacatcacgt cagttttcat aaaggctgtg
      301 aacagccagt aaatcaggcc actgaatacc agccacagca caaccagggc tgcaaccctc
      361 acagggatat cacactcttc cggcactttt tccacagcca tactgcacag atggactgtc
      421 ataacagaaa tcatcacaac agcaaggata tgaatgaaaa gaaaaacgac tttcattttc
      481 cggaaatgca tatacaaacc ctgactcagc ctgtcctgtc tttcctgatc ctctgtaatc
      541 ttaacttatc catgcgctga cgaagtctgc ccggaacacg cccgctcttt cttgaaatat
      601 gagcacagat aaaatcttca agcagaaaat gcatgtcctg cattaccaca tcttcttttt
      661 tgtctttcac tgaaaaatgc ctgacaaaat cgctcatgaa aatgtttctg aaacgctgtt
      721 atccagcttc agaaaccggt actgccttca ccaacacaaa tcattttgtt gtcactgtct
      781 gacctgttct cccctcccgg aactcgctgt tactgatagg aaaatgtaaa tgttgatatt
      841 ccgttaaccg tacccggatt aattccctca ccagtattca catactttac atacaaccgg
      901 actgagggtg acgtttccgt tcctgttgat aactgccact ggttttcatt tccctttacc
      961 ggcgaatcag gtccaaactt caggggcgta ctgtcagtgt ttttgtatat tctcagcccg
     1021 actccggttg cagtcgatgc ccccgtcagt gtgagtatat cgcttctgtt ggacggtgtg
     1081 gtcgcatcag tcagtgttgc ccatactgtt actccggcat cacactgtaa tttcagtgat
     1141 gttgtcccgg cgacaacttc atcaccggat gatggcagtt gtccggcaga aattgcagcc
     1201 aggctgacat tgactgcgga aggtgtcagt aatctgcacg atgttactgt gtcattcagc
     1261 gtaaatggat tcaggctgaa attcactgtg gtctgaacac tgcctgcaga tgaaccgtta
     1321 tagggcctca ttctgatact cccgatgctg gtcttctgta cactgtatcg cgcagaggaa
     1381 tttcttttta aaattacagg tgttacacga acagacagtc cctgggttga tgcagtatta
     1441 gctccgacac accatccgga aaagacattt tcccatgagt ttccgctcat cgcggtaccc
     1501 ctggaataat tcgtgtcccc catccatata aggagcccca cctcctctgt cagcccataa
     1561 atactctgcc cgtcaagact gccaaccacg ggtaaatcca ctttcccgtc cagcccgaca
     1621 caaaatccct tagaaatacg ccagacattt gcattctgat atccaatgtt gttaccggta
     1681 ttatagacaa atggcaggtt atccattgca tgagttctgg aataagagcc tggagatggt
     1741 cccacatcgt tttccgtact gccaataaat gaaactgccg cctgactgat attgcagcaa
     1801 accagaatga atacagccag aaagacctta taaaaatttg tcatattatt gcctcatgca
     1861 tactacatga tggttttccg gaacagcatt tcccctggca ttttctccgg acgcactata
     1921 gtgaatgaca caactgtcat tctgcccgct tccccagact atcctgagca tccctttttc
     1981 agcatccagg tcacgtacaa ataaccggcc tccctgagca atgtagcctg cagatttccc
     2041 gtctgctgtt aatgcttccg cggccatcgg cggcggcgtt ccgtccggca tcttcacatc
     2101 aaacaacatg gctttccccg tttttgttgt gaaggtcacc atggtggtac tgttggcccg
     2161 gggaataatt tcccgctctg ttgcagacag ttccacatcc gcagccagcg ctgtcgggtc
     2221 gatactgata taattctttt cgtaaggtgt gacatacgga acaatgccgt taccccaggg
     2281 gtccagccgg ttacccggcg cattattaat caccgcccca ccggcaccat cagcatgaat
     2341 aacggtaaat gtgtcaccaa ggtcattact ggccgtcaca ccaaacggat gcgccactat
     2401 tgccccggat ataccggcag atatctgacg gttatggcga ctgtcattac ctgccgtggc
     2461 ctgcagggtg gccaccggtg aacgataact gccattcacc gcataccctg tcggtccgtt
     2521 ttcctgggtg ttcagggaca caccatatga caactgattg tcatccccgc tgacaccact
     2581 gatactgctc tgaatgctgt tactgttttt atcgctgaca ttcactgtcg tggacagcaa
     2641 cgtccttccc gtatctgaaa gtggcaggga aaaactgaaa aagaaccggt tatcatgatg
     2701 ataatcacca ctccgtcggg tctgggaaaa gccaatctgg taactcattt ttttccagac
     2761 gttgttataa ccggcctggt actcattgaa tgtccctgta tgtccccagt aatgataagt
     2821 ggctccggtc agatacaggt ttccccatcc ctcaccaaga ctctggttca cagtcagttg
     2881 caactgattc cttggccggt aaccagttgc actcctgacc gatgaatcat taatcacatc
     2941 acgattcctg gtcaggatgg catcccgaag actccagaag tcacgggatg aataacggta
     3001 agccgccagc gtcacatttg tactggtttc cggtattcgt gcactgtaac cggcatgcag
     3061 gctgtagccg cgatgtgtcg tgtcagaacc cgggaaagtc gttctggcca gcgtaatatc
     3121 cgtggcaaca gcccccagtg gtgtgttgaa tgccacacca cccagccctg acagataatg
     3181 aggtgcggta ctcatccccg tattcagggt gatatcattt gtcagcccgt actgcagggt
     3241 cccctggaat acggtatcgc tgaatgtcct gttaccgtaa cgataacgcc cggctgccac
     3301 ctgccagcga ctgaaccccg ggcgaaccag ttgtgcaacc gaagcaaacg gcacgataaa
     3361 actcctggtc tgtccgtctg actctgtcac agtgactgtc aggtcaccac catatccact
     3421 gggataaagg tcactgatgg agaaaggccc tgccggcacg gtagtttcat aaataatgtt
     3481 accgttctgc cggacagtaa ccctggcgtt acttccggcc atcccccgaa ccacaggggc
     3541 ataaccacgc aacgaacccg gcagcatccg gtcatcagaa gccagtctta ttcccctgag
     3601 gctgttactg tccataagct cacctgtggt ggtgaaatcc cccagtgtca gttgcgcacg
     3661 caacatggct atgtcgtgct gaaggttcgt ttcaatactc cggtaacctt ctgcatgacc
     3721 gtcactccag ctttctgtcc cccggtgacg aaatgcccag cctcctgcgt taaagccggc
     3781 acgaagacca agataactct gattattttc catatcgggc gtccggtaac gccagtgatt
     3841 cacgtcataa ctgacaaagg cagccggcac ccccgtctgc cactgagaag gtgatatgta
     3901 tccccgtgga cgggaaacca gcatggcctg cggaatttca acattcagcc gcagcagtga
     3961 caggtcaaaa cggatttttg catcaggtac aactgcagaa aatggcatgc aacggccatt
     4021 tccggtatcc ggttcacggg caactgcctc gggtgacaca tccagaatgg ataacagttc
     4081 cggcgtcaca cacagaacac tctcctcctg tgaagaggac atatcacgga atgacaattc
     4141 cgcatttcct ttccattcac cgttcagata aatgtctgaa taataattcc ctgagggaac
     4201 aggattcccg tgcgtgaaac gacggacatc aatcacactg ctccccggcg tgcggctcag
     4261 aaaactcggg tcaaattctg catcctctgc cgcccacaaa ggcacagaca gcatatacag
     4321 tcccgacaga caacataaat aaatgacaca gtacttcaga ccggataaag aataatgact
     4381 cacttacggg cccttattat ttcagaacgg attcaccttt ctggtaacca ccattatcat
     4441 tcaccaccgt ccattccact tttcctgacg gagaaacctt ctgcttaagt ctgaaagtga
     4501 cagaagaaaa cggtgcggcc attcccccct gagtactgct gactttctga gctccctgtc
     4561 cgacactgac agtactgaag gtaataaaat aaggcgtggg attactggcc tgaagggtat
     4621 caccgcctgt aagctgccat gtcactttct tatatgcatc atcaggagaa aaaggcagac
     4681 catcaggacg gaaaaaaaac ttaatccggc tgcgtacggc aaactgaagg taattatatc
     4741 cctgtgcttt ttcagatttt ccggcaaaag aaggtttggc cggaatatca agaacattca
     4801 gccagaacag tgattccctg tctgccggaa gtttttctcc cgtatacata attcgcattg
     4861 tctgtccgga aagcggctcc atccggaaaa caggaggcgt aataataaac ggcaccctga
     4921 cagagtcagg aggggaagac gggtcccctg tatcaagcca tgcctgtatc agggatggcg
     4981 tactgtctcc gttattcagc cggacattaa tacttttttg ttctgcagga taaataaccc
     5041 tggtccccat aataaccaca ctgccatatg agggcagaga caaaagaacg gtaaaaatga
     5101 acagcagaat atgtttcata caggctccgt aaacacgggg caaaatgccc cgtgaacact
     5161 taagaaggca ggtattaaga taaaaattac tgataagcga tggtgtaatt cacagtggca
     5221 gaaacgttgc cggcggtggc cttacccgtt gcatagtacc gtgcgttata acgtaacgtc
     5281 acatttgtac cattaatagc aacaggtgtt accgcctgac cagcagaatc agtaccgagt
     5341 aatatcggtg tggtaccatc ggcattcaga agctggatct gaacatttgt tgcattacca
     5401 gagccgcttg caatattaag gttatgacta tcataatcag tggtcgcatt aggctcaaaa
     5461 taggccttca cattctcagc acctgctgaa gcaggactgg tacagccttc cagtgaaatt
     5521 gtgaaagggg taaggcctgc cacctcccca tttgaagtta atgaattaga ggagacagtt
     5581 ggtagcgtaa ctgtcaaatt cttgctttct gtcgtaacag aacaagtttg atcaacaact
     5641 tttccattaa aagtaattgt accgtcataa gcaagcgtag cagaagatga cgcagccgcc
     5701 agtattccaa ggataaattg aattttccgc atagaatcct cagtgaaac
//
LOCUS       DAPPNJ010000240         5737 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000240 DAPPNJ010000000
VERSION     DAPPNJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.242"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            6..725
                     /locus_tag="RPM73_004027"
     CDS             6..725
                     /locus_tag="RPM73_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDX6522322.1"
                     /translation="MALFALPFMASATASELSIGAGAAYNESPYRGYNKNTKAIPLIS
                     YEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDKRDSTAMA
                     GVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENF
                     SDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEIT
                     DSPMIDRNESFTFVTGVSWRF"
     gene            complement(747..935)
                     /locus_tag="RPM73_004028"
                     /pseudo
     CDS             complement(747..935)
                     /locus_tag="RPM73_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            939..1040
                     /locus_tag="RPM73_004029"
                     /pseudo
     CDS             939..1040
                     /locus_tag="RPM73_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1072..1767)
                     /gene="araD"
                     /locus_tag="RPM73_004030"
     CDS             complement(1072..1767)
                     /gene="araD"
                     /locus_tag="RPM73_004030"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDX6522323.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1761..2621)
                     /locus_tag="RPM73_004031"
     CDS             complement(1761..2621)
                     /locus_tag="RPM73_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDX6522324.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2614..3276)
                     /gene="ulaD"
                     /locus_tag="RPM73_004032"
     CDS             complement(2614..3276)
                     /gene="ulaD"
                     /locus_tag="RPM73_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDX6522325.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3273..4769)
                     /gene="lyxK"
                     /locus_tag="RPM73_004033"
     CDS             complement(3273..4769)
                     /gene="lyxK"
                     /locus_tag="RPM73_004033"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDX6522326.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4773..5693)
                     /gene="yiaO"
                     /locus_tag="RPM73_004034"
     CDS             complement(4773..5693)
                     /gene="yiaO"
                     /locus_tag="RPM73_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDX6522327.1"
                     /translation="MAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPD
                     STLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKV
                     GDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAFKV
                     FGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKYLSLTHHAYSPLLVVI
                     NKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAF
                     SDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1225 a         1579 c         1580 g         1353 t
ORIGIN      
        1 atattgtggc gttatttgcg ttgcctttta tggcaagcgc aactgcttct gaattatcca
       61 ttggtgctgg tgcggcttat aatgaatcgc cttatcgcgg ttataataaa aatacaaagg
      121 caattccgct gattagttat gaaggtgatt ctttttatgt tcgtcagacc acgttaggtt
      181 ttattctgtc gcaaagtgaa aaaaatgaac ttagcctgac cgcatcctgg atgccgctgg
      241 aatttgaccc tgccgataat gacgattatg ccatgcaaca gcttgataag cgtgatagta
      301 cggctatggc gggggttgcc tggtatcacc acgagcgttg gggaaccgtg aaagcctctg
      361 cggctgcgga cgttctggat aacagcaacg gctgggtggg ggagctatcg gtattccaca
      421 aaatgcagat aggtcgcctg tcgctgacac ctgcgctggg cgttctctat tatgacgaga
      481 atttcagtga ctattactat ggcatttcag agagtgagtc ccgtcgtagc ggtctggcaa
      541 gttattccgc gcaggatgcc tgggtgccct atgtcagcct gacggcaaaa tacccgatag
      601 gagagcacgt cgtattgatg gcgagcgcag gatacagcga gctgccggaa gagattaccg
      661 acagcccgat gattgatcgt aatgagagtt ttacctttgt caccggggtg agctggcgtt
      721 tttaattcac cggtggatgt cggtgcggct ggtgctccgt cgtaatgcca caaaggttgt
      781 tgctctggat tgctctgttc ataccgggaa cctggcgtct gaatcaggat cttatggctg
      841 aacaatccct tgccttcaaa tgtcgtaaat ggcgcatcgg cgggcagcaa caggttatgc
      901 agtgactcga cgttgtcggc ctgctccatg caatacggtt taatgacgtt ggctatttcc
      961 ggcagatttt tcagaaacat actggtttaa cgccagcagc gtggaagcgg cgttacagca
     1021 aggaacatat caattcgtag tgccggggcg atgaagcccc ggcgtgaggg attactgccc
     1081 gtaataggca ttggccccat gcttacgcag gtagtgctta tccagcagtt cgttttgcat
     1141 cgcagggagc tgcggcgcaa gctggcgcga gaatagaccc atataggcgc attcttcgag
     1201 tactacggcg ttatgcacgg catcggcggc gtttttaccc catgcgaacg ggccgtgaga
     1261 atgcaccagc accgccggga tttgtgccgg actcctgcca cgttcttcga aggtttcaat
     1321 gatcacttcg ccggtctgat attcatattc gccgttaatc tcctctgcgg tcatctgtcg
     1381 cgtgcagggg atagcaccgt aaaaataatc ggcgtgggtg gtgccccagg cggggagatc
     1441 cagcccggcc tgtgaccaga tggtggcgtg gcgcgagtgg gtatgcacaa tgccaccaat
     1501 ttcggcatag cgacggtaaa gtgccagatg cgttggcgta tcggacgagg gctttttgct
     1561 accttccacc accttgccgc tggctatctc aaccaccacc atatcgtcgg cggtcatcac
     1621 gtcgtactcg acgccggaag gtttgattac catccattgc cgcgtgtcgt ctaccgcgct
     1681 gacattgccc caggtgaacg tcaccagatg gtgagcggga agcgccagat tcgccgccag
     1741 cacgtcggct ttcagttgct ctaacatata aatccagcct cctgcatacg cgcttcaatc
     1801 cagcgccgcg cctgaatgat ctccagcacc ggctctttgg ctttttcggt ccacatctca
     1861 atcaggaaag aaccgcgata gttcagttca tgcagcgttt tgaagatgcc aacgaaatcg
     1921 acgcagcctt cgccaaacgg cacatcacgg aactgtccgg ggctttgccc ggtgactggc
     1981 tgggtgtctt tcaggtggat cgcggcgata cggtcaatgc ccagtttcag ttcggcggga
     2041 acatcattgc cccaggcgct gaggttgccg acgtccgggt agacggtgaa ccacggcgag
     2101 gcgagcatct cgtcccattt tttccatttg ctgatggagt tcataaacgc ggtatccata
     2161 atctccaccg ccagcattac ttgtgatgcc gccgcctgtt cgactgccca cgccagccct
     2221 tcagcaaaac gttgccgggt gccttcgtcg tggtcttcgt aatagacgtc ataacctgcc
     2281 agctggatgg tgcgaatgcc gagatcgcgc gccaggcgaa tcgctttact catgatttcc
     2341 cgcgcccgtt cgcgcactgc ctcgtcacgg ctaccaaagg gaaagcgacg atgtgcggac
     2401 aggcacatcg acgggatccc aacacctgtt tcgatcatcg cggcaaccag cgaagtcctt
     2461 tgtgcggtgc tccagtcaag acgtgaaagc cgttcgtcgg tttcatccac cgacatttcg
     2521 acaaaatcaa aaccgcagct ttttgccagc accagccgct ccggccagga gagatctttc
     2581 gccagcgcct tttcataaat ccctaacgga tgattacgca cgcttgcctc cccagatagc
     2641 gtcgatttgc gcatggaaat cgccagccac ctgcgccgga tttgctgagc ctgccagtgc
     2701 tcgcccggca ataaacgctt tcacgcgaat atctttaaac agcggcaggt cagcaggggt
     2761 aatcccgccg gtaatggaaa gctcaaggcc gatatcggaa agcgccttca tgcgtgccag
     2821 atcggcttcg ccccactgtt gcccgctggc ctgtgcatca cgaccgcgat gataaatcgc
     2881 ctgccgcacg ccaatacgat gccagtcgcg ggcgtcgtcc agcgtccagt taccgaacag
     2941 ctcgatctga atttcaccac cgcagcgttg cgccatcgcg tggccttttt ccaccgtcgc
     3001 gagcggtgcg gcgcagatga tggtcatcca gttagcgcct gcgccaaatg cctgttgcgc
     3061 gagcgtttca ccagcgtcgg cgaccttcca gtcggcaacg atgattttgt cagggcactg
     3121 ttcgcgcaag gctttcaccg cgccaagccc ttcgtttaaa cagagaatgg tgcccgcttc
     3181 gacaatatcg acgctgtctt ttaactgcgt cacgtcgcgc tgtgcggctt caagtgatga
     3241 gtggtcgagt gccagttgca gaagtggtcg gctcataata tgtgctcctt gatgcgggcg
     3301 tgaaagccct gaagtgcggc aatgagatgc tgatagcgtt ggtatttttg ttggtaaagc
     3361 tgatggacag tcatatccgg cagcagggtg cgcaccgggt gctgtaaatc acgctgggct
     3421 tcgctgaaat cgcgataaac cccggtgccg acgcgggcgg caagtgctgc accaaagcag
     3481 ccggtttctt ccacctgcgg cagctcaata cgcagaccgc tgacgtccgc cagcatttgc
     3541 atccagacat cggaatgcgc cgggccgcca gtgacgcgca gggtgtgcac atcagtaaaa
     3601 cgttcgcgca tccggttgag atgggtcata tggctgaaca ccaccccttc ataaatggcc
     3661 tgcaacaggt gtgcgcgggt gtgaatggcc tgcatcccgt agaaaccgct ggtcatctcg
     3721 agtccggcgt tgctgccgta caggaacggc aggaaaaaga gatcgccacc ggccttcggc
     3781 aagctggcaa cggcctggtt gatctcatca aacgagattt ctccccactg tgcggtaaac
     3841 cattcgaggt tgccggaaga ggtggggctg gcttcgtgaa cgataaattc accatcgtta
     3901 acgtagcgac catagacata cggatgcgct tcaccgtcac gtaaaccgcg ggttatgccg
     3961 ctggtcaccg cccaggtgcc catcaccgca ttaagggtaa attcgtcttc gatcccggcg
     4021 cagagtgcgg tggaaaccac atcaaacagg ccgccgacaa cgggcgtacc cgctttcaga
     4081 ccggtcagta cggctgtctg agcggtgatc tccccgcaga tttcggcaga tccgacaaca
     4141 ggcggcaggg cgtgattgat ttcagcaatc cccagccagt cggtgaggca cgggtcatat
     4201 tccccaagac tcatgttgta gaggttggac tcggaaatat tactctcttc acagcctttg
     4261 acgccggtta aacaccagcg caggtagtcg tgcgtcatca tcacgcagcc aatttgcgcg
     4321 tagcgttccg gttcgtgctc tttaagccaa cgtaacagcg ataccggatg cccggtccac
     4381 aaggtttgtc gggtcagcgg gtagagtttt tccgggatgc catcttcctg ccagcgacga
     4441 acgatttcca tcgcccggcg atccgaggat aaaatggcgt taccgagcgg cttgttgttt
     4501 ttatccagca aaaacaagcc ttttccttgt gcggagatac cgataccgac aatttgttcc
     4561 ccgctaacgc cggaatgggt aagcagttcg cgaatgacag ccatgcagca ttgccacagt
     4621 tctgccatat cgcgctctgc ccaacctggc tgcgggctta atgcgcacag cggcaggcgc
     4681 tgcacgcctg cctcccggcc ttcgcggtca tacagcccgg ctttcagcca gctaccgcca
     4741 caatctaacc ccagccagta ttgcgtcatg gtttattgca cctcatccac ggctttcagt
     4801 agatcagcac cctgcggaac atcttttaca aacatgtcgc ggacctgagt acccagtgcg
     4861 tcgctaaagg ctttgcggtc gagatcggtg atgacttcca cgcccgcttc tttcatgccg
     4921 tcgatgattt tttgctgatc ttcggcaacc agtttgcgct ggtagttacc cgcttcctgc
     4981 gcagatgaaa ttagcgcttg ctggaactcc gggcttaagc catcaaactt cgctttgttg
     5041 atcaccacca gaagcgggga ataggcgtgg tgcgtcagag aaaggtactt ctgcacttcg
     5101 aaaaattttg ctgaccagac gacgttgatc gggtgttcct gagcgtcgat agtgcgggtt
     5161 tccagcccgg tatagacttc ggcaaacggc atcgggatcg ggttagcgcc aaagactttg
     5221 aatgcggcga tattcatcgg gctattgttg gtgcgaattt tcagcccttt caggtcggcg
     5281 ggggttttaa ccggtgcgcg cgagttggtg acatcgcgcc agccgttttc ccagtaggcc
     5341 agtactttta gtcctttacc ttcaagtgag gctttcagat catcaccgac tttgccgtcg
     5401 agcgttttat gcgcgtgagc ggtatcgcgg aacaggaaag ggacatcaag caagttcatc
     5461 actggtgata acccggcaaa gttattcgag ccggacattt ccatatcgat ggtgccgcca
     5521 cgtacgccgc tgatcatcgc ctgcgcgtta ccgagggtgc tatccggaaa caattttaat
     5581 ttcagctcgc ctttggttct ctcctgcaat aaatcattga attttttcgc cgcaatatgt
     5641 tgcgagtcgg tttgtgaggt ttcataaccg aaacgtaaag attgcgccgc cagagaagat
     5701 gtgctgaatg cagccaggcc agcaatgaat aatgcgt
//
LOCUS       DAPPNJ010000241         5636 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000241 DAPPNJ010000000
VERSION     DAPPNJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.243"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..651
                     /locus_tag="RPM73_004035"
                     /pseudo
     CDS             1..651
                     /locus_tag="RPM73_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(620..2356)
                     /gene="aceK"
                     /locus_tag="RPM73_004036"
     CDS             complement(620..2356)
                     /gene="aceK"
                     /locus_tag="RPM73_004036"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDX6522328.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2637..3941)
                     /gene="aceA"
                     /locus_tag="RPM73_004037"
     CDS             complement(2637..3941)
                     /gene="aceA"
                     /locus_tag="RPM73_004037"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDX6522329.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3971..5572)
                     /gene="aceB"
                     /locus_tag="RPM73_004038"
     CDS             complement(3971..5572)
                     /gene="aceB"
                     /locus_tag="RPM73_004038"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDX6522330.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1348 a         1493 c         1410 g         1385 t
ORIGIN      
        1 tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc tattgatgtt
       61 aaaagaatct gtggatgaaa taacccctcc attatcacct gcaacgatga atttgctaat
      121 ggcaattggt cagaatcacc aacttacgca actgatgatt cagctccaga aaatgccaga
      181 actacataga acagaaatgt tgactgccta taatagtgga catatgaacg ttattaatac
      241 tatttttaac gcattaccca ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag
      301 cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc gatacagcat
      361 aaacaacaaa acgttgtaga gacggtttat cttgctttat ctgaccatgc acgcctgttt
      421 ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca aaaatactct
      481 gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat ccttaataca
      541 ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat tgcggagaaa
      601 aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta aatctttgcc
      661 tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt tgtagtgcgc
      721 gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg
      781 ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc gagacgctgt
      841 accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat
      901 tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc ccgtgacggg
      961 tgacaccgaa gtttttaaac agcatgtcgc caggaaaaat gttagcagcg gcaagctggc
     1021 gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca
     1081 gccagatatt gagcggcacc atccgccgct cgatatagag atggcgaatt acaatctgct
     1141 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aatgccgggg
     1201 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     1261 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     1321 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     1381 ataccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     1441 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     1501 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     1561 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     1621 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     1681 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     1741 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     1801 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     1861 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     1921 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     1981 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     2041 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     2101 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     2161 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     2221 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcacct
     2281 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     2341 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     2401 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     2461 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacgttc
     2521 ggcaacggct gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt
     2581 gccggatgcg gcgtgaacgc cttatccggc ctacagtcag caacggttgt tgttgcttag
     2641 aactgcgatt cttcagtgga gccggtcagc gcggtgactg aagacgtgcc gccctgaata
     2701 atagtcgtca ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta
     2761 tagccatctt tcgcggcggc aaattccggc tgctgcactt tctcaacgta gtgcttcata
     2821 ccctcgccct gggcataggc gtttgccagg tcaaacatgt tgaaccacat gctgtggata
     2881 cctgccaggg tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg
     2941 gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc
     3001 agcagtttgc ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga
     3061 tccggcgtgg aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg
     3121 ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgcca
     3181 gtaataaatt cactgtcata cgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca
     3241 tcggtacgag caaccagcag ggttggaacg cccgtcacgt cagctgcaag acgcgccgcg
     3301 accagtttct gaatagcttc ttgagttggc accaaaactt tgccgcccat gtgaccgcat
     3361 ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc
     3421 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
     3481 atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcctgc ggaccattgg
     3541 atctgatcgg cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt
     3601 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
     3661 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
     3721 ccagtcagtg caccgaggct gttgatgtag ccttttttcg actcgccatg cagcagacgc
     3781 cacattttcg ccgcgcccag ttgcgccagg gtgcattcag gattgactga accgcgtaat
     3841 ttcaccacat cttccgcgct gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac
     3901 tctttctgta attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt
     3961 atgtggtggt ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat
     4021 cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac
     4081 gttcttcgcc cagttcgctg gcaatgactt tcatctcttc acccagcatc tggcggaaca
     4141 aggctttggt caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga
     4201 ttgaggtacg ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc
     4261 agccgttgcc ggagatccat gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca
     4321 taccttcttc ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggca
     4381 cgtcttgttc gcgcatcact tcaagctgat ttttacggga gccgagaatg tcgttgaata
     4441 ccgccattgc cgtatccgca aggcctgggt gagcaatcca tgtgccatcg tgaccgttat
     4501 tggcttccag cgctttatcc gcttttactt tgttgagcac ctggttattg cgctcttcat
     4561 ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg
     4621 ttttaatcaa caggcgtgag taagcattca ggaatggttt atccatcgtc actgcctgtc
     4681 tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat
     4741 cccaacgacc gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca
     4801 tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca
     4861 gattaaagcg atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgccagg
     4921 actgggtttt cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt
     4981 tgtggaagaa atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg
     5041 tgacatgttt ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg
     5101 gcttgagctg ataaattttg cctgcttcat tggtatagct gatggtgccg ttaaccgcat
     5161 cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga
     5221 aatcggccat aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcaa
     5281 ccgggccagt tatctcgact cgacgatctt gtaagtccgc gggaatacca cgaattttcc
     5341 agtccgtatc gcgaatggaa gctgtttccg aaataaaatc aggcaaagtt ccgttatcaa
     5401 tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg
     5461 tcaccagctc agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc
     5521 catacggcct tatgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc
     5581 tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt gcgctt
//
LOCUS       DAPPNJ010000242         5625 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000242 DAPPNJ010000000
VERSION     DAPPNJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.244"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            109..819
                     /locus_tag="RPM73_004039"
     CDS             109..819
                     /locus_tag="RPM73_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX6522331.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            861..3512
                     /locus_tag="RPM73_004040"
     CDS             861..3512
                     /locus_tag="RPM73_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6522332.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3526..4056
                     /locus_tag="RPM73_004041"
     CDS             3526..4056
                     /locus_tag="RPM73_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6522333.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4069..4572
                     /locus_tag="RPM73_004042"
     CDS             4069..4572
                     /locus_tag="RPM73_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6522334.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAVDVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4631..5545
                     /locus_tag="RPM73_004043"
     CDS             4631..5545
                     /locus_tag="RPM73_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522335.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1614 a         1222 c         1260 g         1529 t
ORIGIN      
        1 cattaagtct ggaaacccgg cgactcgcca atgtattagc aacattaatc gattgtctga
       61 tgtaacggtt ttcccgctta tcctcttaac acatggagaa tgcatcagat gcaaactacg
      121 cgtaccccct actctatttc ctttatggcg acagtgttac ttttattgct ctttgcttgc
      181 catagcaccg ttgccaacgc agctgtcgca ctgggtgcga cgcgggtaat ttatccggca
      241 aaccagaaac aggttttatt gcctgtcacg aataatgatc ctgccagtgt ctatctcatt
      301 cagtcatgga tagaaaatgc aggtgaccaa aaagacaccc agtttgtcat taccccaccg
      361 cttttttcca tgcaaggtaa aaaagagaat acgctgcgga taatcaacgc tactaatcat
      421 cagttaccag gcgaccgtga aagcctgttc tgggtgaatg ttaaagctat tcctgctatg
      481 gaaaaagacc agaagaatga gaatacatta cagctggcaa ttattagccg cataaaaatg
      541 ttttatcgcc ccacgaacct ggcaatggca cctgaagaag cgcctgcaat gcttcggttt
      601 cgtcgcagcg gtagcaaact cacgcttatt aatccaacgc cctatttcat taccgtaaca
      661 aatatgaaag caggaaatag caatctccca aacaccatgg tgccgccaaa aggtgaagta
      721 tcggtggata tcacgcatgc tgcaaccggg gatatcagtt ttcagacgat caatgattac
      781 ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag ccagtgacct aattaataat
      841 aaactcaatc ggaattaata atgacagcca ttcgtgccgc atttaaggca tatcgtatgc
      901 atcaggtgtt aataatgcct cgctttgcac ggcttaccat tgcccttggc ttagcaactg
      961 cggtatttcc cgttgacgca gagtattatt ttaatccacg gtttttaagc aatgatcttg
     1021 ctgaatctgt cgatttatcc gcctttacta agggacgcga ggcaccacct ggtacatatc
     1081 gggtcgacat ttatttgaac gatgaattta tgactagtcg ggatattact tttattgcag
     1141 atgataataa tgcagaactc atcccatgcc tgagtacaga tcttcttgtc agtcttggta
     1201 taaaaaaatc agcgttatta gacaataaag aacattcggc agaaaaacat gtgccggaca
     1261 acagcgcatg cacaccactt caggatcgtt tggccgacgc atcaactgag tttgatgttg
     1321 gtcagcaaca tttgtcactc tccgttccgc aaatttatgt tggcagaatg gctcgcggct
     1381 atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg gcttctaaac tatagtttta
     1441 atggtaattc tattaataat cgtagtaacc ataatgcagg aaaatcaaac tatgcatatt
     1501 tgaatttaca gagtggcatc aacattggta gttggcgact gcgcgataac tcaacgtgga
     1561 gttattacag tgggagtagc aattcatctg acagcaataa atggcagcat atcaatacgt
     1621 cggctgaacg tgacattatt cccttacgct cacggttaac ggtaggtgat agttataccg
     1681 atggcgatat ttttgatagt gtgaactttc gtggcctcaa aataaattca acagaagcga
     1741 tgttgcccga tagccaacat ggtttcgctc cggtgattca tggtattgcc cgtggaaccg
     1801 cacaagtgag tgtaaaacag aacggatacg atgtttatca gactactgtt ccacccggcc
     1861 cttttactat tgatgatatc aactctgcgg ccaatggtgg tgatctgcaa gtaaccataa
     1921 aagaggcaga cggcagtatt cagacattat atgttcctta ttcgtctgtt ccggttctcc
     1981 aacgtgctgg atatacgcgt tatgcgcttg ccatggggga atatcgtagt ggaaataacc
     2041 tgcaaagctc tcccaagttc atacaaggta gcttgatgca tggtctggaa ggaaactgga
     2101 caccttatgg cggaatgcaa attgcagaag attatcaggc cttcaacctt ggtattggga
     2161 aagatttagg actttttggt gccttttctt tcgatatcac gcaggccaat acgacacttg
     2221 cagatggcac ccgtcacagt gggcaatcgg ttaaatccgt ctacagcaaa tccttctacc
     2281 agacaggaac caatatccag gtcgcaggat atcgctattc tacgcaaggt ttttataact
     2341 tatccgacag tgcctacagt cgaatgagtg gttacaccgt caagcctcct accggagaca
     2401 ccaatgagca gacacaattt attgattatt ttaatctgtt ctacagtaag cgtggtcagg
     2461 aacaaataag catctctcag cagcttggaa attacggtac gacatttttc agtgccagtc
     2521 gccaaagtta ctggaacacg tcacgcagcg accagcaaat atcatttgga ttaaatgtgc
     2581 cgtttggtga tattacgact tcgctgaatt acagctattc caataatata tggcaaaacg
     2641 atcgggatca tttactcgct tttacgctta atgttccctt cagtcattgg atgcgtacag
     2701 acagtcagtc ggcatttcgt aattcaaacg ccagttacag tatgtcaaac gatttgaaag
     2761 gcggcatgac caatctatcg ggggtttatg gcactctgct gccggataat aacctgaatt
     2821 atagcgttca ggtcggtaac acccacggag gtaatacatc gtctggcacc agtggttaca
     2881 gttctcttaa ttatcgtgga gcttacggta atacgaatgt cggttacagt cggaatggtg
     2941 acagcagcca gatttattac ggaatgagtg gtgggattat tgctcatgct gatggcatca
     3001 cctttggaca gccgctgggc gacacaatgg ttctggttaa ggctcctggc gctgataatg
     3061 tcaaaataga gaaccagacc ggaattcata ccgactggcg tggctatgca atattaccat
     3121 ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc gaattccctt gcagataatg
     3181 ttgaactgga tgaaaccgta gtcactgtca tcccaactca cggtgctatt gccagagcaa
     3241 catttaatgc acaaatcggc gggaaagtat taatgacgtt gaagtacggt aataaaagcg
     3301 ttccattcgg tgcaattgtc actcacggag agaataaaaa tggcagcatt gtcgcggaaa
     3361 atggtcaggt ttatctgact ggacttccac agtcagggaa attacaggtt tcatggggca
     3421 atgataaaaa ctcaaactgt attgtcgatt acaagcttcc tgaagtctct cctggaacct
     3481 tgctgaacca gcagacagca atctgtcgct aaggaacaat agattatgaa atacaataac
     3541 gttattttcc tcggtttatg tctggggtta accacctatt ctgctttatc cgcagatagc
     3601 gttattaaaa ttagcgggcg cgtcctcgat tatggctgca cagtctcatc ggattcgctt
     3661 aattttaccg tagatctcca aaaaaacagt gccagacaat ttccaacgac cggtagcaca
     3721 agtccagccg tcccttttca gattacgtta agtgaatgca gcaaagggac aacgggggtt
     3781 cgggttgcat ttaacggtat tgaggatgca gaaaataata ctctgttgaa actggatgag
     3841 ggaagcaata cggcctccgg tttaggtata gaaatactgg acggaaatat gcgtccggtg
     3901 aaactgaatg accttcatgc cgggatgcag tggatcccac tggtaccaga acagaacaat
     3961 attttgcctt actccgctcg tctgaagtca actcagaagt ccgtcaatcc gggactggtg
     4021 agggcttcgg caacctttac ccttgaattt caataatcag agtcagatat gaaaagactt
     4081 cacaagaggt tcctgttagc tacgttttgc gcgttattca cagcaactct ccaggccgtc
     4141 gatgtcacta tcactgttaa tggtcgggta gtcgctaaac cctgcactat tcaaaccaaa
     4201 gaagctaacg ttaatctcgg ggatctttat acgcgcaatc tgcaacaacc tggttctgca
     4261 tctggctggc acaatattac tttgtcatta accgattgtc cggttgaaac aagtgcagtg
     4321 acggcaatcg tgacaggttc aactgacaat acgggttatt acaaaaatga aggtactgcc
     4381 gaaaatattc agatagagct gagggatgac caggatgcga cgttaaaaaa tggcgatagc
     4441 aaaacggtta ttgttgatga gatcactcgt aatgcacagt ttccacttaa ggcaagagct
     4501 atcacggtga atggaaacgc aagccaggga acgatcgagg cgctaatcaa tgtgatctac
     4561 acctggcaat aacgtcgatt cgattttctt agccttcttt ttaaatcaat ttttaacctt
     4621 aggatatatc atgggtaaaa caatcagtat aaaggtcctg ttcggtattt atcttctatt
     4681 aatggcaggc aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa gtattggagc
     4741 aggtacaacc tctgtttatg ttaatctcga ccctgtaata cagccgggcc agaatctggt
     4801 tgtagacttg tctcagcata taagttgctg gaatgattac ggcggctggt acgacactga
     4861 tcatataaac ctggtacaag gctcagcttt cgcaggctcc ctacagtcat ataaaggatc
     4921 gctgtactgg aataatgtga cctacccctt cccgttgacg acaaatacca acgtattgga
     4981 tattggtgat aagacaccga tgccattacc gttaaaactc tatattaccc cagttggggc
     5041 ggccggtgga gttgtcataa aagcggggga agtcattgcc cgtattcata tgtataaaat
     5101 tgcaacatta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt caaataacaa
     5161 tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc
     5221 ggactttcct ggaagcgcag aaatacccct tggcgtatat tgctcaagcg aacaaaagct
     5281 ctcattttat ctttcgggtg ccaccactga cagtgcccga caagtgtttg ctaacacagc
     5341 accagatgca acaaaagcat cgggagtggg tgtctctctg atgcgtaatg gaaaaatact
     5401 ggctacggga gaaaatgtct ccttggggac agtaaataaa agcaaagtac ctctgggttt
     5461 aagcgcaacc tatggacaaa cagggaacaa agtttcagcc ggaaccgtcc agtcagtcat
     5521 tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc gctccattca
     5581 cgaaaattgc gcggataatt attttgtgaa agctattatc ctaca
//
LOCUS       DAPPNJ010000243         5600 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000243 DAPPNJ010000000
VERSION     DAPPNJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.245"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..866
                     /locus_tag="RPM73_004044"
     CDS             <1..866
                     /locus_tag="RPM73_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDX6522336.1"
                     /translation="ISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDL
                     LIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLE
                     LTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLD
                     FLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTRE
                     ATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRV
                     QSTSKTAPQAK"
     gene            complement(1059..1412)
                     /locus_tag="RPM73_004045"
     CDS             complement(1059..1412)
                     /locus_tag="RPM73_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDX6522337.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1550..3196)
                     /gene="groL"
                     /locus_tag="RPM73_004046"
     CDS             complement(1550..3196)
                     /gene="groL"
                     /locus_tag="RPM73_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDX6522338.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3240..3533)
                     /locus_tag="RPM73_004047"
     CDS             complement(3240..3533)
                     /locus_tag="RPM73_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDX6522339.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3810..5066
                     /gene="yjeH"
                     /locus_tag="RPM73_004048"
     CDS             3810..5066
                     /gene="yjeH"
                     /locus_tag="RPM73_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDX6522340.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5082..5558)
                     /gene="fxsA"
                     /locus_tag="RPM73_004049"
     CDS             complement(5082..5558)
                     /gene="fxsA"
                     /locus_tag="RPM73_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDX6522341.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
BASE COUNT         1325 a         1473 c         1347 g         1455 t
ORIGIN      
        1 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
       61 tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg
      121 aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg
      181 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      241 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      301 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      361 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      421 ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag
      481 atttcctgcc gttatatgac gttgactgcc aggaaaatgg caatctggaa tatgacacct
      541 attcccaacc ggaatggaaa cataacctgt tcgaccatta tctggcggtg ctttaccgct
      601 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      661 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga
      721 aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc
      781 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca
      841 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
      901 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
      961 ccggcctaca aattctcaag cattcaatca gttgcaatgc cttatgtagg cctgataagc
     1021 gaaacgcatc tggcaattat acgtttgtca ccatcacatc agttcgggca cttataaacc
     1081 tgaccgataa tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggctg
     1141 ctgatgccat aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct
     1201 gcgccgcgca tagaaccgcc ttcttctccg tgttgcccgg aaagccagtt gctttgctta
     1261 cctgtcgcag taccaatcag ctggcactct gcgcctggct gctcgtccac aatgcgtaca
     1321 ctctgaccgg cagcactcaa ttcgttgctg gagctacaac ccgccatcag tagcgcggca
     1381 ccgacaatcc ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac
     1441 gccactcgcg tcgtccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc
     1501 cagacatttc tgcccggggg tttgtttatt tctgcgaggt gcagggcaat tacatcatgc
     1561 cgcccatgcc acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt
     1621 tcggcaggtc ggtaaccatg cactcggtgg tgatcatcag gccagccaca gaagccgcgt
     1681 actgcagagc agaacgggtt actttggttg gatccaggat acccatgtcg atcatgttgc
     1741 cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acagtgttag
     1801 caacaacaga cggttcttcg ccgcagttca atacgatctg acgcagcgga gcttccattg
     1861 cacgcagtgc aactttgata cccacgtttt ggtcttcgtt ctgaccacgc aggtcagcca
     1921 gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg
     1981 cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc atttcaactt
     2041 cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct
     2101 gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa
     2161 cacggccctg gattgcagct tcttcaccca cgccatcaat gatggtggtg gtgtctttgt
     2221 tgatcacaac acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac
     2281 cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt
     2341 tacgacgatc gccgaagccc ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg
     2401 tgttaacaac cagagttgcc agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg
     2461 gtttgcctgc tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttagagattt
     2521 tcttgtcagc cagcaggatg aacggacttt ccagttctac tgcgccagtt tccggcttgt
     2581 tgatgaagta aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt
     2641 cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca
     2701 tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagatg gtaccaacct
     2761 gagcaatcgc tttagagtca gagcacggta cggacagcgc tttcagttct tcaactgcag
     2821 cggtaacggc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag
     2881 ctttcagacc ctcagtgatg atagcctgag ccagtacagt tgcagtggtg gtaccgtcgc
     2941 ctgcagcgtc gttcgcttta gaggcaactt ctttcaccat ctgcgcaccc atattttcga
     3001 acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg
     3061 caccgaaaga tttatccaga actacgttac ggcctttcgg accgagggta actttcactg
     3121 catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta
     3181 cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgaggat
     3241 tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg
     3301 atcttctcag atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcaca
     3361 tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg
     3421 ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt
     3481 tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac
     3541 tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg
     3601 ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa
     3661 ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc
     3721 ttcatcaccg tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca
     3781 aaatttgggc gcaatcggat atcaaaccca tgagtggact caaacaagaa ctggggctgg
     3841 cccagggcat tggcctgcta tcgacgtcat tattaggcac tggcgtgttt gccgttcctg
     3901 cgttagctgc actggtagca ggcaataaca gcctgtgggc gtggcccgtt ttgattatct
     3961 tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc agcgcaggcg
     4021 gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc accggctggc
     4081 tgtttttatc agtcattccc gtgggtttac ccgccgcact acaaattgct gccgggttcg
     4141 gccaggcgat gtttggctgg catagctggc aactgttgtt ggcagaactc ggtacgctgg
     4201 cgctggtgtg gtatatcggt actcgcggtg ccagttccag tgccaatcta caaaccgtta
     4261 ttgccggact tatcgtcgcg ctgattgtcg ctatctggtg ggcgggcgat atcaaacctg
     4321 cgaatatccc cttcccggca cctggtaata tcgaacttac cgggctattt gctgcgttat
     4381 cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc tcggaattta
     4441 aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg tctgctgctg gcaggattag
     4501 tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa caaatggcgg
     4561 cggcagcatc gcttccaaaa attgtagtgc agttgttcgg tgtcggagcg ttatggattg
     4621 cctgcgtaat tggctatctg gcctgctttg ccagtctcaa catttatata cagagcttcg
     4681 cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca cgcctctctt
     4741 ctcgccatat cccgaataat gccctcaatg cggtgctcgg ctgctgcgtg gtgagcacgt
     4801 tggtgattca tgctttagag atcaatctgg acgctcttat tatttatgcc aatggcatct
     4861 ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa ggacgttatc
     4921 gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg gtcggctgga
     4981 aaagtctcta tgcgctgatc atgctggcgg ggttatggct gtttctgcca aaacgaaaaa
     5041 cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatcctg gcgatcgtct
     5101 ttatgatcaa ggcggtcgcg ctcatcatcc tttcgctggt actcaccatc aaaagtatta
     5161 ccgccaccgg tcccggcgct aaaaccgccg cctggcatac gagaaaagcg caaatgcggc
     5221 atcaacttca ctgtcagatg cttttgcacc ggcggtaata aaagcagaag gccgaggaag
     5281 tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa tgatcagcga aacactttta
     5341 atcatctccg ccgctgggtt ttcacccgcc gccatttttt gctgcatcag cacgaaattc
     5401 ttaaagccct ggttacgtac cagtgacatc ccgataactg acgtgaatat aaccagcacg
     5461 agggtcagca atacccccaa tacatgggca acctgaataa agattgaaat ctcaatatag
     5521 acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg taatagcagc
     5581 cggtaaaacc cggctacctg
//
LOCUS       DAPPNJ010000244         5592 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000244 DAPPNJ010000000
VERSION     DAPPNJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.246"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(80..799)
                     /gene="dcuR"
                     /locus_tag="RPM73_004050"
     CDS             complement(80..799)
                     /gene="dcuR"
                     /locus_tag="RPM73_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDX6522342.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(796..2427)
                     /gene="dcuS"
                     /locus_tag="RPM73_004051"
     CDS             complement(796..2427)
                     /gene="dcuS"
                     /locus_tag="RPM73_004051"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDX6522343.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2608..2838
                     /locus_tag="RPM73_004052"
     CDS             2608..2838
                     /locus_tag="RPM73_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDX6522344.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2850..3122
                     /locus_tag="RPM73_004053"
     CDS             2850..3122
                     /locus_tag="RPM73_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX6522345.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFSKHEFDKTREYDDIRS"
     gene            3349..3645
                     /gene="ghoS"
                     /locus_tag="RPM73_004054"
     CDS             3349..3645
                     /gene="ghoS"
                     /locus_tag="RPM73_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDX6522346.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3673..3846
                     /gene="ghoT"
                     /locus_tag="RPM73_004055"
     CDS             3673..3846
                     /gene="ghoT"
                     /locus_tag="RPM73_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDX6522347.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3965..5482)
                     /gene="lysS"
                     /locus_tag="RPM73_004056"
     CDS             complement(3965..5482)
                     /gene="lysS"
                     /locus_tag="RPM73_004056"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDX6522348.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         1462 a         1289 c         1319 g         1522 t
ORIGIN      
        1 actgatgaag gatgtaactt gtgccagggg tattttgcat tacggtaata attattactt
       61 acagataacc agcgatccgt tattggcaat attgtttcag tagtgagtag tgttctgcct
      121 gaatacggta acggtaaacc ggacgccccg tgacgccata atggatactg gtgaacaaga
      181 tgtggcagtt gaccagccag atgaggtatt tacggcagga aacacgcgag atgttaacct
      241 cgttagccag ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca
      301 gcgtgcgtaa cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc tgttcgttgg
      361 agctgctgcc gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca
      421 gcgccatttt cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg
      481 gtttgatcag gtaatccacg acaccgtaat gcagcgaatc tttaatggtt gccgcatcgg
      541 ctgcggagga gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta
      601 aatcgagccc gttctctttt tgcatataga tatcgagcaa tatcaggtcg ataggcgtat
      661 cgctattgaa gataatctct ttggctttct ccagcgtcga ggctgttcca cagcattgaa
      721 agcctgggat ttgtgctacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga
      781 taattaatac attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa
      841 ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg
      901 ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc
      961 aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcgcagtg
     1021 cagccagccg tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggccctaa
     1081 tgcttccagc gcgttttcta tcagatttcc caatgtggta atcagcgtcg cgacctggtc
     1141 ctcactgccg ctgtctggca gctggctttc actgtttaaa atcagcgtat ggcctaaatc
     1201 ggtcgcgcga ttaatcttgc tgattaaaaa accagcgata accggagatt tgatcttacc
     1261 cagcagagag ccaatctctt cctgatagtt attggctgtt ttgagaatgt aatcttccaa
     1321 ctgcttataa ctcttcagat gcaataatcc gagaatcaca tgcaatttat tcataaattc
     1381 gtgggatcgt tcacgaagtg catcagcata gttgaccaga ccgtcgagtc gctgcatcag
     1441 tttacgaact tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact
     1501 gcgcaccgga acggtgttga tcagtaatag ccgatcttta atcgtaatct cttcgtcgcg
     1561 gcgcggcgta ccgtcgcgta acacttccga gacatctacc acctgtgacc atgagtggct
     1621 gagcgtcgac agtttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc
     1681 atcgttgatc agcgtgacct cgccgcgatc gtccacggca acgacgcctt ctttgataga
     1741 ctgcaacatg gcctggcgct gctcaaacag cgtggagatt tcgtagggtt ccaggccgaa
     1801 aaggattttt ttcagtacct taaccagaat gcaggtgcca atcagtccga ccagcatgcc
     1861 aaataatacc gaccagataa tgctccagcg actgtcattg atttgttggg tcacgcggct
     1921 taactcaagg ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt agatgggggt
     1981 aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata gcgacatttt ctttgccatt
     2041 cagcgcttta aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg
     2101 cgagtagcga agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg
     2161 cacggcttcc gcgatggcct ggattccgct ctcctgcggt tttttctgca agccctgacg
     2221 gatttccggc gagtcggcga gggtacgcgc cactgccagt gccttgttgg ctagcccgtc
     2281 tcgcgtcata tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa
     2341 cagtaccgca ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa
     2401 catgcggtag ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt
     2461 atctctgatg aggcgggtaa ttcaaaggga gtaagaatga ttggctatat aggggaagag
     2521 actctggcaa cggaaactgc cagtgctgta tgaagattcc agggctatgc ttatagcgat
     2581 aatcatactg atgagagagg gaaagtcatg gatcaggcgc tactggacga gggttatcgc
     2641 tgttataccg gcgaaaagat cgatgtctat ttcaacactg cgatatgtca gcattctggc
     2701 aattgcgtac gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg
     2761 gatgaagtcg acgtcgtcac tgtggttaaa gtgattgata cgtgcccgag cggcgcgctg
     2821 aaataccgtc ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataaatttt
     2881 acattaatga cgaacagggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga
     2941 atttagcgat tatcgaacat accgatgtcg atgaaagcct gaaagggcag gggattggta
     3001 aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccac
     3061 tatgcccatt ttcgaaacac gaatttgata aaacgcggga gtatgatgat attcgcagtt
     3121 gatgggagag tacagagtca cgatattttt catgctctcc gcggagtgat gcaggagagc
     3181 atctgaaggg tagggggatg cacaaagaat gggcagagag agcgtttttt tgtcccaagt
     3241 catccccttt actgagcaaa aaaaagaata tctcctatat gagaatcatc aatcggggtt
     3301 aataagtttt gcgtccccag agcgtttaat attgatagga gtcatattat ggaaggtaaa
     3361 aacaagttca atacttatgt tgtttctttt gattatccat catcttattc ctcagtgttc
     3421 ttaaggttaa gatcattgat gtatgatatg aatttctcct ctatcgtggc tgatgaatat
     3481 gggataccac gacaattgaa tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt
     3541 gagatcgaag atttaatcag gcttaaatgc ttagacttac cggatattga ttttgacctc
     3601 aacattatga cagttgatga ctatttccgt cagttttaca agtagccagg aaggataaga
     3661 agaacataaa aaatggcact attctctaaa atattaattt tttatgtgat tggtgtgaac
     3721 atatcctttg tcattatctg gtttatctca catgagaaaa cacatattcg tttacttagt
     3781 gcattcctgg tcggaataac ctggccaatg agtctgcctg tggcattact tttttctctc
     3841 ttttaggagc tgtagttgag tggacccgct ggttatcgtg aaacgaagcg tctctcctca
     3901 aaactggatt tgcggaacga cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg
     3961 aaatttattt ctgtgggcgc atcgccggga agagaataac gtcgcgaata gtatgactgt
     4021 ttgtaaacag cataatcatt cggtcgatac caatacccag accagcggtt ggcggcagac
     4081 catattccag cgcagtcacg taatcttcgt catagaacat ggcttcgtcg tcacctgcag
     4141 ctttagcgtt aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc
     4201 taaaaccatt accgatttca cgaccaccga tgaagaattc aaaacggtcg gtgatttccg
     4261 ggttaacatc attacggcgc gccagcggtg acacttctgc cggatattcc gtaataaagg
     4321 ttggctgaat cagatgtgct tctgccactt catcaaagat ctccgtgaca atacgtccca
     4381 gcccccagct tttctctacc gtaattccga tagattcagc taatgcttta gcagcatcaa
     4441 aattatccag gtcggccata tcggtttctg gacgatattt tttgattgct tcgcgcatgg
     4501 tgagtttttc aaacggtttg ccgaaatcaa acacatgctc gccataagtg actttagtgg
     4561 tacccagaac ctcttgtgcc agggtgcgga acagggactc tgtcagttca atcaaatcgt
     4621 ggtaatccgc atacgccatg tagagttcca tcattgtgaa ctcaggatta tggcgaacag
     4681 aaataccttc attacggaag ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa
     4741 ccagacgttt cagatacagc tccggcgcga tacgcagata catatctaaa tccagagcat
     4801 tatgatgggt aataaacggg cgagcagatg ccccacctgg aattacctgc atcatcgggg
     4861 tttctacttc cataaagccg cgcgcgacca tgaactgacg gatagcggcc agaatttttg
     4921 aacggacaac gaacgtttga cgggatttat cgttagcgat gaggtccaga taacgttgac
     4981 gataacggac ttcctgatcc tgcagaccat ggaatttatc tggtaaagga cgtagtgctt
     5041 ttgtcagcag gcgcaactca gtacagtgaa tggaaagctc accggtttgc gtcttaaaca
     5101 gcgtaccgcg ggcaccgata atgtcaccca gatcccattt tttaaactga tcgttataaa
     5161 caccttctgg caggctatct cttgcaacgt acagttgaat acggccaccg acatcctgca
     5221 acgttacaaa ggaggctttc cccatgatac gacgggtcat cattcggcca gcaaccgaga
     5281 cttcaatgtt taaggattcc agttcctggt tattcttcgc atcaaactct tcgtgcaact
     5341 ggtcagaggt atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg
     5401 ccgccagttt ttcgcggcga tttctcagtt catcgttaaa atcaatagcc tcattggctc
     5461 cccgtgtttc ttgttcagac attttggttc ctctaaatcc agctttcaat ttagcgtaga
     5521 taaagagaca gatcggtaca ctgtattatc tgcctcgact attaataact cagtttatct
     5581 tatcgaaact aa
//
LOCUS       DAPPNJ010000245         5491 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000245 DAPPNJ010000000
VERSION     DAPPNJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.247"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="RPM73_004057"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="RPM73_004057"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDX6522349.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="RPM73_004058"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="RPM73_004058"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDX6522350.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /locus_tag="RPM73_004059"
     CDS             complement(3139..3438)
                     /locus_tag="RPM73_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDX6522351.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="RPM73_004060"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="RPM73_004060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDX6522352.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..5087)
                     /locus_tag="RPM73_004061"
     CDS             complement(4650..5087)
                     /locus_tag="RPM73_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDX6522353.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWYAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5133..5474)
                     /locus_tag="RPM73_004062"
     CDS             complement(5133..5474)
                     /locus_tag="RPM73_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522354.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1327 a         1422 c         1355 g         1387 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccaaaatg
      361 ttggagacac gcttgttcgc cgccgccagt gcagcagctg cttcaagggt acggaagtgc
      421 gataccgctt tcatgcgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aaccacgttg gcgttagtca gcttatcgcc atacagacgc
      601 actgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg
      721 ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct
      781 accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc
      841 accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtgt tgaagaagaa ctcggcatcc
     1141 gccagacgcg gacgaacgac tttctcgtta ccggagataa tctgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat aaagttcggc agcagtttgc cgtcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accagcgctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct
     1741 tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctgaaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc
     3361 tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caactgaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacct gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tatccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgcct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata
     4861 taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac
     4921 gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc
     4981 atctctgcat accatagccc taacagatag gtaacgatac caccaacgag agctatccat
     5041 gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt
     5101 tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga
     5161 ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt
     5221 gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata
     5281 tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca
     5341 aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag
     5401 atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt
     5461 tttgaggttt tcataacgat ctccatatct a
//
LOCUS       DAPPNJ010000246         5410 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000246 DAPPNJ010000000
VERSION     DAPPNJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.248"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            155..270
                     /gene="rrf"
                     /locus_tag="RPM73_004063"
     rRNA            155..270
                     /gene="rrf"
                     /locus_tag="RPM73_004063"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(502..1260)
                     /locus_tag="RPM73_004064"
     CDS             complement(502..1260)
                     /locus_tag="RPM73_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDX6522355.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1268..2371)
                     /locus_tag="RPM73_004065"
     CDS             complement(1268..2371)
                     /locus_tag="RPM73_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDX6522356.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKIPPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2381..3562)
                     /locus_tag="RPM73_004066"
     CDS             complement(2381..3562)
                     /locus_tag="RPM73_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDX6522357.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3630..4655)
                     /locus_tag="RPM73_004067"
     CDS             complement(3630..4655)
                     /locus_tag="RPM73_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6522358.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5086..5307)
                     /locus_tag="RPM73_004068"
     CDS             complement(5086..5307)
                     /locus_tag="RPM73_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522359.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1498 a         1396 c         1289 g         1227 t
ORIGIN      
        1 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt
       61 acaacgccga aggtgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      121 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggccttagcg cggtggtccc
      181 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      241 tccccatgcg agagtaggga actgccagac atcaaataaa acataaggct cagtcggaag
      301 actgggcctt atgttttatt tgatgtttgt cggtgaacac tctcccaacc aggacaaatc
      361 cgccgggaac ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
      421 cgccataaac tgccagacat caaatcaagc aaaaaggcca tccgaaagga tggccttttt
      481 gcttttcgaa ctaacattca attaatggat tacctgcgat aaaaatgccc tcgtacgctc
      541 tgatttagga tgcgtaaaaa attcatcagg tgcagcttgc tccactattt ccccacgatc
      601 cataaaaatc acccggtcag cgacggttcg cgcaaacccc atctcatgcg ttacacacaa
      661 cattgtcata cccgattgcg ccagcccaat catcgtatcc agcacctctt tcaccatctc
      721 cggatcgagc gccgacgttg gctcatcaaa caacataatt ttcggcttca tacacagcga
      781 acgcgcaatg gcaacgcgtt gctgctgacc accagaaatt tgtccgggaa acttatgcgc
      841 atgttcggca attctcaccc gctcaaggta atgcatcgcc agagcttcag cctctttctt
      901 tggcatcttg cgtacccaaa tcggtgccag ggtacagttc tgtagaacgg tcagatgagg
      961 gaagagattg aaatgctgaa agaccattcc cacttcctgc cggacgcgct caatattgcg
     1021 gatatcttca ttaagttcga tgccatctac cacgatccgt ccctgttgat gttcttccag
     1081 atgattaata caacgaatgg ttgtcgattt accggaacct gaagggccac acagaacgat
     1141 ccgttctccc ggttgcacgg ttaaatttat atttttcaaa acatggaatt gtccatacca
     1201 tttattgacg ttttccagcg taatcatcgc gttagcaggt tgcagtaaaa tttggctcat
     1261 agtgtcctca atgcggtgta cgcccggtgt taaaacgttt ttccagatgc tggctatagc
     1321 gcgacatgct gaaacagaag atccagtaga tcagcgcggc gaaaacatac ccttccgtcg
     1381 acatacccaa ccaggtggga tcaacggttg cctgctgaac gctactgaaa agatcgaaca
     1441 acccgataat gatcaccagg ctggtatctt tgaagagtgc gatgatggta tttaccagcc
     1501 caggaattac cagcttcaac gcctgtggca gaataaccag cccctgagtt ttccagtaac
     1561 ccaacgccag cgactctgcc gcttcatact gccctttagg cagtgcctgt aatccgcctc
     1621 gcacgacttc cgcaacatat gctgactgaa acaggatcac gccaaccagc gcgcggatca
     1681 gtttgtcgat actggtgcct tctgccataa acaacggcag catgaccgaa gacataaaca
     1741 atacggtaat cagcggtacg ccgcgccaga actcgataaa aatgaccgat aagatacgca
     1801 caatcggcat atgggagcgg cgacctaacg ccagtaagat cccccacggt agcgcccccg
     1861 caatcccaac ggatgcaata attaaagtta gcgtcagccc gccccattgc cgggtttcaa
     1921 cccgctccag accaaaaaaa ccgccataca tcagccacca gacaatcagt gggtaaatca
     1981 ccgcccagac cgcaatatag cgaccgcgat gcgggggtat tttccagaac attggtgcga
     2041 ttgacacaag gccaatcagt aatgccaggt taattcgcca gcgttggtcg tgtgggtaaa
     2101 gtccatacat aaactgacca aatcgttcgt ggatgaagac ccaacaggca ccggcttttg
     2161 tgcagtcggc acgcgttgag ccaacccaat tagcctgcaa aaatgcccag ttcagcaacg
     2221 gtgggatcag ttcccacatc aaccatatgc agccaatagt cagcaggcta ttgctccagc
     2281 tggagaacag attttttcgc acccacacca tcgcgcggct tgagttatgg ctcgctgggc
     2341 gcgagggctg agacagcaat acttttgtca tcatgactcc ttagcgttca acgatcgcga
     2401 tgcggcggtt atagatattc atcagcagcg agatagtcag gctgataatc agatagaccg
     2461 acatggtcat ggctatcgtc tcgatggctt gccccgtctg gttcagcacg gtgccagcaa
     2521 acagcgaaac catatcggga tagccaatag cggcggcaag agaggagttt ttgacgatgt
     2581 tgagatactg gctggttaac ggcggaataa tcactcgcag tgcctgggga ataatgacct
     2641 ggcgtagcgt aaccgggttg ggtaatccca gtgaccgagc cgcttcatgt tgaccataag
     2701 gcactgcctg gatcccagcg cggataatct cggcgataaa tgcagatgta taaaccgaaa
     2761 gtgccagcgt taaggctgcc agttcaggaa ttaaaaccat cccgccgcgg aagttaaagc
     2821 ctcgtagggc tgggacatcc cagtgtagtg ctgcgccaaa aagccattgc gccagtaaag
     2881 gcaaaccaat gatcaacact gcggcgatcg gccaggtgcg gcgcagttgt ccggtcttta
     2941 tctggtatgt tttattaaag cggaatagcc caacagaaag gactatagcc ataacaacag
     3001 ccaggataaa cgcaataaat ccgtctccca gctgcggtga cggaatataa agcccacgat
     3061 tgctcaaaaa ggccagatca aacgcgctca cagcctggcg tggtccgggc aaattgcgca
     3121 acacggcaaa gtaccagaag aagatttgca gcagcggggg aatattacgg aagatctcaa
     3181 tataaattgt ggaaagcttt cgtagcagcc agttatccga aagtctcgcc agaccgataa
     3241 agaagcccaa aacagaagcg aacacaatac acaatgcaga aaccagtagc gtattgagta
     3301 agccgacaat aaaaacgcgt ccgtaggtgt cgccctgctg gtaatcgatc aaatgctgga
     3361 caataccgaa gccagcgccg cgatccagaa aggcaaaacc tgaagtaatg ccacgattac
     3421 tgagattcgt tacagtgttg tgaaataacc aaccaacaat gccgacaaca gcaacaacgg
     3481 caaggatctg gaataaccag gcacgaaccg tagggttggc aaaggagagt gagcctttaa
     3541 cggttgagcg gcgatgagac atagaaacct cggaaagcat aacgctgaag agagcgccgc
     3601 cgtagcgacg ctcgttacct cacaccgact taacgcacgg gcggcgcgta ctgaataccg
     3661 ccgttattcc agagattatt ttgcccacgt ttaattttca gcgggctttc tgaacctaca
     3721 ttacgctcga aaatttccga gtagttaccc acctgtttga tgatgttata ggcccattta
     3781 ttatccaact tcagatcctt gccgtaatcg ccttctttac ccagcagatg tgccatatca
     3841 ggcgttgctg gattagccgc tttttcatcg acgttctggg aattgatgcc catctcttca
     3901 gcattcagca tggcgaaaag cgtccagcgt acaatcgaga accattcatc atcgccacga
     3961 cgaactaccg gcccaagcgg ttctttagag ataacctccg gtaagacaat ccattcagct
     4021 gggttgctta atttgatgcg cagggcatac agttgcgact gatccgaggc cagcgtatcg
     4081 cagcgaccag attccagtgc cttcgctgat tcgtcagagc gatcgaaagt caccggtgtg
     4141 tacttcatat tgtttgcctt gaaatagtcg gcaacgttga gttcggtatc agtacccgcc
     4201 tgaatacaga cggtagcgcc atccagttct ttcgcgcttt ttagccccgc tttatcgtgc
     4261 gtcaggaagc caatgccgtc gtaataagtg acgccagtaa atgccattcc catcccggcg
     4321 tcgcgagatg aagtccaggt cgtattacgg gagagcaaat ccacttcccc tgactgtaaa
     4381 gccgtgaagc gttcttttgc tgtgagtggg gtatatttca ctttcgtgtc gtcaccaaat
     4441 acagcagcgg caacaccacg acaaacatca acatcaatac ctgaaaactt accgtctgca
     4501 tcggcataag agaacccagg taatccatca ctgatcccgc attgcacaaa accttttttc
     4561 tgaacggcat caagcgtcgc gccagcatgc gcctgatttg caacggcaag cagcacgctg
     4621 gcggcagcca gtgtggctat catcatcttt ttcataatgc ttcctgtgag gcgaaaatta
     4681 tcgttatagg acgtttggga aacgtctttt acctgtctgt ggccatcctc ttataagcaa
     4741 aagtagtgcc agcttttgcg cccgatttcg cgtcgcaata agctgagtgt agacaggaaa
     4801 taataaaaac agcgccccgc aatggtgcga aaatgaaaca tgcgccacat tttggcgcaa
     4861 aatatttacg cgtgttttac taagcagaat aatgattgtt agcgtgagta tatttctggt
     4921 gggaagtgtg aaagttaaat tgatgcgtta tttcgaaagc agcgaaataa cgcgatataa
     4981 catgctaaat cagcagagaa ttagccagca gaatatagca atattttatc ttatgtaaag
     5041 atactgtatc gttaaacaga gcgcgaaact ccgcgccctg ttcagttatt tcgacagagc
     5101 gatgatcccc atcgtcaggc cagcaacagc cgctgccccg ccagcaatag ccccagccgt
     5161 ttcccagtta tcctgagtcg tgcctttcat acaggtattg gtgtgaacca gacgaccctg
     5221 atcgtcataa accggaacgc agggcgaatc gtgagcacag cccgccagca atgcggtgag
     5281 caatgccaca ggaattaatc ttttcatggt gttacctcag taataaacgc taacgcttaa
     5341 taagttaact ctcttattaa cgagctgaat gccattttat caccggaggt tataaatatt
     5401 gcggaatgaa
//
LOCUS       DAPPNJ010000247         5406 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000247 DAPPNJ010000000
VERSION     DAPPNJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.249"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            158..1216
                     /gene="citC"
                     /locus_tag="RPM73_004069"
     CDS             158..1216
                     /gene="citC"
                     /locus_tag="RPM73_004069"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDX6522360.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCNWLHLFLVKEDSSRFPYEGRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1231..1527
                     /gene="citD"
                     /locus_tag="RPM73_004070"
     CDS             1231..1527
                     /gene="citD"
                     /locus_tag="RPM73_004070"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDX6522361.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1524..2432
                     /gene="citE"
                     /locus_tag="RPM73_004071"
     CDS             1524..2432
                     /gene="citE"
                     /locus_tag="RPM73_004071"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDX6522362.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2443..3975
                     /gene="citF"
                     /locus_tag="RPM73_004072"
     CDS             2443..3975
                     /gene="citF"
                     /locus_tag="RPM73_004072"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDX6522363.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3979..4530
                     /gene="citX"
                     /locus_tag="RPM73_004073"
     CDS             3979..4530
                     /gene="citX"
                     /locus_tag="RPM73_004073"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDX6522364.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4505..5383
                     /gene="citG"
                     /locus_tag="RPM73_004074"
     CDS             4505..5383
                     /gene="citG"
                     /locus_tag="RPM73_004074"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDX6522365.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1238 a         1532 c         1432 g         1204 t
ORIGIN      
        1 ccgaaatact ccttaggatg tatagcgaaa ggagaaaaag atatacctcg atcacccccc
       61 tttctcccaa gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa
      121 atcgataaca acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac
      181 ccgcgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa
      241 tgatttgagc gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct
      301 tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt
      361 tcgcggtgaa ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg
      421 gcacagcacg catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg
      481 cggtttttcc acgctgacca gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac
      541 gcggctgaaa cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg
      601 ctgcattgtg atgaacgcca atcccttcac gaatggtcac cgttatctga ttcaacaagc
      661 tgcggcacag tgcaactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc
      721 ctatgaaggc cggttggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt
      781 gcatcgcggc tccgaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga
      841 acagagcgtc attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct
      901 cgctcccgcg ctgggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac
      961 cgcccagtac aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcaccgcc
     1021 catcgaactg gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg
     1081 ggtacgtcaa ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt
     1141 cacgctgcat tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca
     1201 aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc
     1261 acccttgagt ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac
     1321 ctgcaaatca atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg
     1381 gacgttctcg cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca
     1441 ctggactgca ttttacgtgc acgactggaa gccctgctgg cacgcgccag cggtatcccg
     1501 gctttgccat gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca
     1561 cccgccgcag catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca
     1621 tctacccggc tgatgccctg atgtttgacc tcgaagactc cgtagcattg cgtgaaaaag
     1681 acaccgcccg ccgcatggtt taccacgcgc tgcaacatcc gctgtatcgc gatattgaaa
     1741 ccattgtgcg tgtcaacgcg ctggattccg aatggggtgt caacgacctg gaagccgtcg
     1801 ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg
     1861 atatcgaaaa agagatcctg cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagcaccg
     1921 gcctgctggc ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg
     1981 cttccgagcg tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta
     2041 cagaacgctc cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg
     2101 cgcgctctgc gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg
     2161 gattcctgca agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca
     2221 acccgcgtca gattgatctg ttgcacaacc tctacgcacc aacccagaaa gaagtggatc
     2281 acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt
     2341 ccctgaacgg caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctctc
     2401 gtgcagaact ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa
     2461 caatctcaac gacaagaacg ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc
     2521 gctttccaga actcgccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg
     2581 tgcgccaacc tggaagaagc gattcgtcgc tctggtttac aggacggcat gacggtttcc
     2641 ttccatcacg ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg
     2701 aagatgggct ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg
     2761 ttggtagaac acattcgcca gggcgtggtt acccgcattt atacctccgg cctgcgcggt
     2821 ccactggcgg aagagatctc ccgtggtctg ctggcagaac cggtgcaaat ccactctcac
     2881 ggcggtcgcg tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc
     2941 gtcccgtcct gcgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc
     3001 tccctcggct atgcaatggt tgatgccgac aacgcaaagc aggtcgtgat gcttaccgaa
     3061 gaactgctgc cttatccgca taatccggca agcattgagc aagatcaggt tgatttgatc
     3121 gtcaaagttg accgcgttgg cgatgctgca aaaatcggcg ctggcgcgac ccgtatgacc
     3181 actaacccgc gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactcaggc
     3241 tacttcaaag aaggtttctc catgcaaacc ggcaccggcg gcgcatcgct ggcggtaacc
     3301 cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggtggt
     3361 attaccgcga cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgta
     3421 cagagctttg acagccacgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc
     3481 agcgccaacc agtacgctaa ctggggttcg aaaggtgcat cggttgatcg tctcgacgtg
     3541 gtggtactga gcgcgctgga aattgacacc cagttcaacg ttaacgtgct gaccggctct
     3601 gacggcgtac tgcgtggtgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt
     3661 tccatcatcg tcgcgccgct ggtacgcggt cgtattccga ctctggtgga taacgtgctg
     3721 acctgcatca ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt
     3781 aacccggcac gtccggagct ggcagaacgt ctgcaggaag cgggcattaa agtggtttcc
     3841 attgagtggc tgcgtgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc
     3901 accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtacat
     3961 caggtgaagg aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca
     4021 attcccgagc tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgt ctggctcaag
     4081 cgccatcctg ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagt
     4141 gaggtcacac gccgaatttt taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa
     4201 cagggctggc aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggtatg
     4261 ttgagcattg ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt
     4321 catcctctcg ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc
     4381 cgccgcgact attcactgcc gcctcgcagc tgcctgttgt gcgagcaaag cgcagccgtc
     4441 tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg
     4501 aacgatgtcg atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga
     4561 tgagtacgcc ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc
     4621 aggcctcgtg gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca
     4681 ccgcagcgcg ctggcgattc agggctggtt accccgtttc attgaatttg gtgcctgtag
     4741 tgcagaaatg gcaccggaag cggtactcca cggattacgc ccaattggta tggcttgcga
     4801 aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt
     4861 agggctgcta tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg taacgccaac
     4921 aaccgtttgt tctacggcgg caagtttctg ccgtggtctg accgatcgcg aactgcgtac
     4981 caataattca caactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc
     5041 acgcggtgaa gccgaagcgg gttatccact ggtgatcaac cacgccttgc cgcattacct
     5101 cactctgctg gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact
     5161 gatggcgatc aacggcgata ccaacgttgc ttcgcgcggt ggcgaggggg gcctgcgctg
     5221 gctacagcgc gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccgccgatct
     5281 cgattatctc cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag
     5341 tgccgacctg ctgatcctta cctggttttt agcacagatt taattattta agcacttgat
     5401 aaattt
//
LOCUS       DAPPNJ010000248         5365 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000248 DAPPNJ010000000
VERSION     DAPPNJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.250"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            292..1626
                     /gene="cadB"
                     /locus_tag="RPM73_004075"
     CDS             292..1626
                     /gene="cadB"
                     /locus_tag="RPM73_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDX6522366.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1706..3853
                     /gene="cadA"
                     /locus_tag="RPM73_004076"
     CDS             1706..3853
                     /gene="cadA"
                     /locus_tag="RPM73_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDX6522367.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3912..>5365
                     /gene="dtpC"
                     /locus_tag="RPM73_004077"
     CDS             3912..>5365
                     /gene="dtpC"
                     /locus_tag="RPM73_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDX6522368.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESN"
BASE COUNT         1262 a         1289 c         1307 g         1507 t
ORIGIN      
        1 tgcggcaatt tatttgccgc ataattttta ttacataaat ttaaccagag aatatcccgc
       61 aatccattgt aaacattgaa tgtttatctt ttcatgatat caacttgcga tcctgatgtg
      121 ttaataaaaa acctcaagtt ctcacttaca gaaacttttg tgttatttca cctaatcttt
      181 aggattaatc cttttttcgt gagtattctt agcgccagtt tggtctggtc aggaaatagt
      241 tatacatcat gacccggact ccaaattcaa aaatgaaatt aggagaagag catgagttct
      301 gccaagaaga tcgggctatt tgcctgtacc ggtgttgttg ccggtaatat gatggggagc
      361 ggtattgcat tattacctgc gaacctagca agtatcggtg gtattgctat ctggggttgg
      421 attatctcta ttattggtgc aatgtcgctg gcgtatgtat atgcccgact ggcaacaaaa
      481 aacccgcaac aaggtggccc aattgcttat gccggagaaa tttcccctgc atttggtttt
      541 cagacaggtg ttctttatta ccatgctaac tggattggta acctggcgat tggtattacc
      601 gcggtatctt atctttccac cttcttccca gtattaaatg atcctgttcc ggcgggtatc
      661 gcctgtattg ctatcgtctg ggtatttacc tttgtaaata tgctcggcgg tacttgggta
      721 agccgtttaa ccactattgg tctggtgctg gttcttattc ctgtggtgat gactgctatt
      781 gttggctggc attggtttga tgcggcaact tatgcagcta actggaatac tgcggatacc
      841 actgatggtc atgcgatcat taaaagtatt ctgctctgcc tgtgggcctt cgtgggtgtt
      901 gaatccgcag cagtaagtac tggtatggtt aaaaacccga aacgtaccgt tccgctggca
      961 accatgctgg gtactggttt agcaggtatt gtttacatcg ctgcgactca ggtgctttcc
     1021 ggtatgtatc catcttctgt aatggcagct tccggtgctc cgtttgcaat cagtgcttca
     1081 accatcctcg gtaactgggc tgcgccgctg gtttctgcat tcaccgcctt tgcgtgcctg
     1141 acttctctgg gctcctggat gatgttggta ggccaggcag gtgtacgtgc cgctaacgac
     1201 ggtaacttcc cgaaagttta tggtgaagtc gacagcaacg gtattccgaa aaaaggtctg
     1261 ctgctggctg cagtgaaaat gactgccctg atgatcctca tcactctgat gaactctgcc
     1321 ggtggtaaag catctgacct gttcggtgaa ctgaccggta tcgcagtact gctgactatg
     1381 ctgccgtact tctactcttg cgttgacctg attcgttttg aaggcgttaa catccgcaac
     1441 tttgtcagcc tgatctgctc tgtactgggt tgcgtgttct gcttcatcgc gctgatgggc
     1501 gcaagctcct tcgagctggc aggtaccttc atcgtcagcc tgattatcct gatgttctac
     1561 gctcgcaaaa tgcacgagcg ccagagccac tcaatggata accacaccgc gtctaacgca
     1621 cattaattaa aagtattttc cgaggctcct cctttcattt tgtcccatgt gttgggaggg
     1681 gcctttttta cctggagata tgactatgaa cgttattgca atattgaatc acatgggggt
     1741 ttattttaaa gaagaaccca tccgtgaact tcatcgcgcg cttgaacgtc tgaacttcca
     1801 gattgtttac ccgaacgacc gtgacgactt attaaaactg atcgaaaaca atgcgcgtct
     1861 gtgcggcgtt atttttgact gggataaata taatctcgag ctgtgcgaag aaattagcaa
     1921 aatgaacgag aacctgccgt tgtacgcgtt cgctaatacg tattccactc tcgatgtaag
     1981 cctgaatgac ctgcgtttac agattagctt ctttgaatat gcgctgggtg ctgctgaaga
     2041 tattgctaat aagatcaagc agaccactga cgaatatatc aacactattc tgcctccgct
     2101 gactaaagca ctgtttaaat atgttcgtga aggtaaatat actttctgta ctcctggtca
     2161 catgggcggt actgcattcc agaaaagccc ggtaggtagc ctgttctatg atttctttgg
     2221 tccgaatacc atgaaatctg atatttccat ttcagtatct gaactgggtt ctctgctgga
     2281 tcacagtggt ccacacaaag aagcagaaca gtatatcgct cgcgtcttta acgcagaccg
     2341 cagctacatg gtgaccaacg gtacttccac tgcgaacaaa attgttggca tgtactctgc
     2401 tccggcaggc agcaccattc tgattgaccg taactgccac aaatcgctga cccacctgat
     2461 gatgatgagc gatgttacgc caatctattt ccgcccgacc cgtaacgctt acggtattct
     2521 tggtggtatc ccacagagtg aattccagca cgctaccatt gctaagcgcg tgaaagaaac
     2581 accaaacgca acctggccgg tacatgctgt aattaccaac tctacctatg atggtctgct
     2641 gtacaacacc gacttcatca agaaaacact ggatgtgaaa tccatccact ttgactccgc
     2701 gtgggtgcct tacaccaact tctcaccgat ttacgaaggt aaatgcggta tgagcggtgg
     2761 ccgtgtagaa gggaaagtga tttacgaaac ccagtccact cacaaactgc tggcggcgtt
     2821 ctctcaggct tccatgatcc acgttaaagg tgacgtaaac gaagaaacct ttaacgaagc
     2881 ctacatgatg cacaccacca cttctccgca ctacggtatc gtggcgtcca ctgaaaccgc
     2941 tgcggcgatg atgaaaggca atgcaggtaa acgtctgatc aacggttcca ttgaacgtgc
     3001 gatcaaattc cgtaaagaga tcaaacgtct gagaacggaa tctgatggct ggttctttga
     3061 tgtatggcag ccggatcata tcgatacgac tgaatgctgg ccgctgcgtt ctgacagcac
     3121 ctggcacggc ttcaaaaaca tcgataacga gcacatgtat cttgacccga tcaaagtcac
     3181 cctgctgact ccggggatgg aaaaagacgg caccatgagc gactttggta ttccggccag
     3241 catcgtggcg aaatacctcg acgaacatgg catcgttgtt gagaaaaccg gtccgtataa
     3301 cctgctgttc ctgttcagca tcggtatcga taagaccaaa gcactgagcc tgctgcgtgc
     3361 tctgactgac ttcaaacgtg cgttcgacct gaacctgcgt gtgaaaaaca tgctgccgtc
     3421 tctgtatcgt gaagatcctg aattctatga aaacatgcgt attcaggaac tggctcagaa
     3481 tatccacaaa ctgattgttc accacaatct gccggatctg atgtatcgcg catttgaagt
     3541 gctgccgacg atggtaatga ctccgtatgc tgcgttccag aaagagctgc acggtatgac
     3601 cgaagaagtt tacctcgacg aaatggtagg tcgtattaac gccaatatga tccttccata
     3661 tccgccggga gttcctctgg taatgccggg tgaaatgatc accgaagaaa gccgtccggt
     3721 tctggagttc ctgcagatgc tgtgtgaaat cggcgctcac tatccgggct ttgaaaccga
     3781 tattcacggt gcataccgtc aggctgatgg ccgctatacc gttaaggtat tgaaagaaga
     3841 aagcaaaaaa taattagctc gtacaaggga agtggcttgc cacttccctt ttttgctcat
     3901 aaggagaaca catgaaaaca ccctcacagc cgcgcgcgat atactatatc gtggcgatcc
     3961 aaatctggga gtacttcagt ttttacggca tgcgtgcctt actcattctc tatctcaccc
     4021 atcagcttgg ttttgatgat aaccatgcca tcagcctgtt cagcgcatat gcttctctgg
     4081 tttacgttac ccctattctc ggcggctggc ttgccgaccg cctgctcggc aaccgcactg
     4141 cagtgattgc cggcgcgctg ttaatgaccc ttggccatgt ggtgctgggc attgatacaa
     4201 attcaacctt tagcctgtat ctggcgctgg caatcattat ttgtggctac ggtttattca
     4261 aatcaaacat cagctgtttg cttggcgagc tctacgacga gaacgatcat cgacgtgatg
     4321 gcggtttttc gctgctgtat gctgcgggca atatcggttc tatcgccgcc ccaatcgcct
     4381 gcggcctggc tgctcagtgg tatggatggc atgttggctt tgcccttgcg ggtggcggca
     4441 tgtttatcgg tttgttgatt ttcttaagcg ggcatcgtca tttccagtcc acgcgtagta
     4501 tggataaaaa agcgctcact agcgtgaaat ttgccttacc agtatggagc tggttagtgg
     4561 tgatgctctg tttagcccca gtatttttta ccctgctgct ggagaacgac tggtccggat
     4621 atttgctggc gatcgtttgc ctcattgccg cacaaatcat tgcccgcatg atgatcaaat
     4681 tccccgaaca ccgccgtgct ctttggcaaa ttgtattgtt gatgtttgtc gggacgttgt
     4741 tctgggtact ggcacaacag ggcggcagta ccatcagctt gtttatcgat cgctttgtga
     4801 atcgtcaggc attcaatatt gaagtaccta cagcactatt ccagtcggtg aatgcaattg
     4861 cagtgatgct cgctggggtt gtactggcct ggctggcatc gccagaaagc cgcggcaact
     4921 caacattgcg cgtctggctg aagtttgcct ttggcttact gctgatggct tgtggcttta
     4981 tgttgttggc atttgatgcc cgacatgcag cggctggcgg tcaagcgtca atggccgtga
     5041 tgatatccgg gctggcgcta atgggctttg ccgaactctt tattgacccg gtggcgattg
     5101 cgcaaatcac gcgtctgaaa atgtctggcg tattaaccgg tatttatatg ctggcaacag
     5161 gcgcggtcgc caactggctg gcaggcgtcg tggcacagca gacgacagag tcgcaaatta
     5221 gcggtatggc aattgcagct taccagcgat tcttttctca gatgggagag tggacgttgg
     5281 cttgtgtcgc gatcatcgtc gtattggcct ttgctacccg ttttctgttt agcacgccga
     5341 cgaatatgat acaggagagc aacga
//
LOCUS       DAPPNJ010000249         5303 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000249 DAPPNJ010000000
VERSION     DAPPNJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.251"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            161..859
                     /locus_tag="RPM73_004078"
     CDS             161..859
                     /locus_tag="RPM73_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX6522369.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(904..1803)
                     /gene="eamA"
                     /locus_tag="RPM73_004079"
     CDS             complement(904..1803)
                     /gene="eamA"
                     /locus_tag="RPM73_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDX6522370.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1998..3185
                     /gene="ydeE"
                     /locus_tag="RPM73_004080"
     CDS             1998..3185
                     /gene="ydeE"
                     /locus_tag="RPM73_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDX6522371.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3312..3407
                     /gene="mgtS"
                     /locus_tag="RPM73_004081"
     CDS             3312..3407
                     /gene="mgtS"
                     /locus_tag="RPM73_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDX6522372.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3626..4516)
                     /gene="dgcZ"
                     /locus_tag="RPM73_004082"
     CDS             complement(3626..4516)
                     /gene="dgcZ"
                     /locus_tag="RPM73_004082"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDX6522373.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4771..5163)
                     /gene="ydeI"
                     /locus_tag="RPM73_004083"
     CDS             complement(4771..5163)
                     /gene="ydeI"
                     /locus_tag="RPM73_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDX6522374.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1466 a         1264 c         1120 g         1453 t
ORIGIN      
        1 aatatcatat cccgcagtag ctatacgtcc tggtacattg atcacagtaa tacagtgata
       61 aagttgttca acatcagggt gattattaaa cgactcggcc atacgataaa ccaatcagga
      121 ctcaactcct tgttttcaat ggctcttaaa aggtgcaaaa gtgatttttc aaaagattgc
      181 ccgcttactt aaatccgaaa tcaatggcaa ttcatggcat gttggtgatt tgttgccgtc
      241 agaagcggaa ctcgctgttc gttataatgt ttcgcgtaat accctgcgta aggcattatc
      301 cttgctggaa ggcgaaggaa ttattcacag aaagcatggt tcaggaacat acattcaaaa
      361 aaagaatttt gttgcacaca ttgatcacat gaacagtttc agtgaaattg cgcataaaag
      421 cggcaaagag gcagggagtc agattatgaa atttgaagtg caggatgctt cacccactat
      481 tgcgactgag ctgaatttag tgactggtga gcaggtttat tacataaaga gactgcgatt
      541 cattgaggat aatgcagcac aattagaaga aacgtggatg tcagtagcac gttttcctga
      601 tttaaccgtg tcacatatgc aaaaatccaa attttcatat attgagaatg aatgcgggat
      661 caaaatcatt ggcacctttg aaactttctc ccctactttt cctaccccag aaatcgccag
      721 tattttacgg atcagcccac gggatcccat acttaaaatt cagacccagg ctgtggacag
      781 taactctatt ccgctggatt attcgttact ttacagcaat attttcgagt tccaggtaaa
      841 gtactttttc ccgcgataaa agcagacgca cagagtgctg acatcacatt ctgtgcgtat
      901 ggtttagcct ctaaccttta ccgctttacg ccaccgcaag ccaaatacat tgatatacag
      961 cccggtcata atgagcaccg cacctaaaaa ttgcagaccc gttaagcgtt catccaacaa
     1021 tagtgccgca cttgccagtc cgactacggg caccagtaac gataacggtg caacccgcca
     1081 ggtttcatag cgtcccagta acgtccccca gatcccataa ccaacaattg tcgccacaaa
     1141 cgccagatac atcagcgaca agatggtggt catatcaata gtaaccagac tgtgaatcat
     1201 ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag ggaatgattg ggattaaagc
     1261 gctccagatt accagcgaca tcaccgccgg acgcgttgag tgcgacatga tctttttatt
     1321 gaagatgttg ccacacgccc aactaaatgc tgcagccagg gtcaacataa agccgagcat
     1381 cgccacatgc tgaccgttca gactatcttc gattaacacc agtacgccaa aaatcgctaa
     1441 ggcgatcccc gccaattgtt tgccatgcaa tcgctccccg aaagtaaacg cgccaagcac
     1501 gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc agtccagcag gcataccgaa
     1561 gttaatggca caaaaaagaa aagcaaactg cgcaaaactg atggttaatc cataccccag
     1621 cagcaaattc agtggtactt tcggtcgtgc gacaaaaaag atagctggaa aagcgaccag
     1681 cataaagcgc aaaccggcca gcatcagcgg tggcatgtta tgaagcccca ctttgatgac
     1741 cacaaaattt agcccccata cgaccactac cagtagcgcc aacaccccat cttttcgcga
     1801 cattctaccg cctctgaatt tcatcttttg taagcaatca acttagctga atttactttt
     1861 ctttaacagt tgattcgtta gtcgccggtt acgacggcat taatgcgcaa ataagtcgct
     1921 atacttcgga tttttgccat gctatttctt tacatctcta aaacaaaaca taacgaaacg
     1981 cactgccgga cagacaaatg aacttatccc tacgacgctc taccagcgcc cttcttgcct
     2041 cgtcgttgtt attaaccatc ggacgcggcg ctacgctgcc atttatgacc atttacttga
     2101 gtcgccagta cagcctgagt gtcgatctaa tcggttatgc gatgacaatt gcgctcacta
     2161 ttggcgtcgt ttttagcctc ggttttggta tcctggcgga taagttcgac aagaaacgct
     2221 atatgttact ggcaattacc gccttcgcca gcggttttat tgccattcct ttagtgaata
     2281 acgtaaagct ggtggtgctc ttttttgccc tcattaactg cgcctattct gtttttgcta
     2341 ccgtgctgaa agcctggttt gccgacaatc tttcgtccac cagcaaaacg aaaatcttct
     2401 caatcaacta caccatgcta aacattggct ggaccatcgg tccgccgctc ggcacgctgt
     2461 tggtgatgca gagcatcaat ctgcccttct ggctggcagc tatctgttcc gcgtttccca
     2521 tgcttttcat tcaaatttgg gtaaagcgca gcgagaaaat catcgccacg gaaacaggca
     2581 gtgtctggtc gccgaaagtt ttattacaag ataaagcact gttgtggttt acctgctctg
     2641 gttttctggc ttcttttgta agcggcgcat ttgcttcatg catttcacaa tatgtgatgg
     2701 tgattgctga tggggatttt gccgaaaagg tggtcgcggt tgttcttccg gtgaatgctg
     2761 ccatggtggt tacgttgcaa tattccgtgg gccgccgact taacccggct aacatccgcg
     2821 cgctgatgac agcaggcacc ctatgtttcg tcatcggtct ggtcggtttt attttttccg
     2881 gcaacagcct gctattgtgg ggtatgtcag ctgcggtatt tactgtcggt gaaatcattt
     2941 atgcgccggg cgagtatatg ttgattgacc atattgcgcc gccaggaatg aaagccagct
     3001 atttttccgc ccagtcttta ggctggcttg gtgccgcgat taacccatta gtgagtggcg
     3061 tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat cttagcgttg gtgatcattg
     3121 ctgcgtgggt gctgatgtta aaagggattc gtgcaagacc gtgggggcag cccgcgcttt
     3181 gttgatttaa gtcgaacaca ataaaaattt aattcagcct tcgtttaggt tacctctgct
     3241 aatatctttc tcattgagat gaaaattaag gtaagcgagg aaacacacca caccataaac
     3301 ggaggcaaat aatgctgggt aatatgaatg tttttatggc cgtactggga ataattttat
     3361 tttctggttt tctggccgcg tatttcagcc acaaatggga tgactaatga acggagataa
     3421 tccctcacct aaccggcccc ttgttacagt tgtgtacaag gggcctgatt tttatgacgg
     3481 cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca tggatagatt tgtgttttgc
     3541 ttttacgcta acaggcattt tcctgcactg ataacggatc gttgacacag tagcatcagt
     3601 tttctcaatg aatgttaaac ggagcttaaa ctcggttaat cacattttgt tcgtcaataa
     3661 acatgcagcg atttcttccg gtttgcttac cctcatacat tgcccggtct gctcttccaa
     3721 tgaccacatc cagaggctct tcaggaaatg cgcgactgac acctgctgtc acggtaatgt
     3781 tgatatgccc ttcagaatgc gtgatggcat ggttatcgac taactggcaa attctgacac
     3841 ctgcacgaca tgcttcttca tcattagccg ctttgacaat aatgataaat tcttcgcccc
     3901 cgtagcgata aaccgtttcg taatcacgcg tccaactggc taagtaagtt gccagggtgc
     3961 gtaatactac atcgccgatt aaatgcccgt aggtatcatt aaccaattta aatcggtcaa
     4021 tatccaacaa cattaaataa agattcagag gctcagcgtt gcgtaactga tgatcaaagg
     4081 attcatcaag aacccgacgc cccggcaatc ccgtcaaaac atccatattg ctacggatcg
     4141 tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa agaaagcaac ccctcctgaa
     4201 aggcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat agccagcatt aattcccgac
     4261 cacagttatg catatgttga tgggcagaat ccattagccg aacgtaaggt aattcatcgt
     4321 tatcgagtgg ccccagatga tcaatccacc gaccaaactg gcacagtcca taagaatggt
     4381 tatccgttat ttctggctta ctggcatctc tcgcgaccac gctgtgaaac atactcacca
     4441 gccactggta gtgggcatcg atagccttat tgagatttaa caagatggca tcaatttccg
     4501 ttgttttctt gatcattgcc actccttttt cacagatcct tgtgcgcgct attctaacga
     4561 gagaaaagca aaattacgtc aatattttca tagaaatccg aagttatgag tcatctctga
     4621 gataacattg tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt ctgtaaatta
     4681 cctctgcatt atcgtaaata aaaggatgac aaatagcata acccaatacc ctaatggccc
     4741 agtagttcag gccatcaggc taatttattt ttatttctgc aaatgagtga cccgaacgac
     4801 ggccggcgcg cttttcttat ccagactgcc gctaatgttg atcatctggt ccggctgaac
     4861 ttctcgtcca tcaaagacgg ccgcaggaat aacgacatta atttcaccgc tcttatcgcg
     4921 aaaaacgtaa cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg aaaccgaagc
     4981 gccatcgtgc atggtatttg cgaaatcaac ggtcattttt tttgcatcat cggttccgcg
     5041 atagccatct tctattgcat gaggcggcgg tggcgctgca tcctgtttta aaccgccctg
     5101 gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg cctgaaattt
     5161 catactgact ccttaattgc gtttggtttg acttattaag tctggttgct atttttataa
     5221 ttgccaaata agaatactgc caattgttat aaggcattta aaatcaggca actagctgtc
     5281 aaatattcag agaatttaac tca
//
LOCUS       DAPPNJ010000250         5289 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000250 DAPPNJ010000000
VERSION     DAPPNJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.252"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..190)
                     /locus_tag="RPM73_004084"
     CDS             complement(<1..190)
                     /locus_tag="RPM73_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDX6522375.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQ"
     gene            complement(393..698)
                     /locus_tag="RPM73_004085"
     CDS             complement(393..698)
                     /locus_tag="RPM73_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522376.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            723..962
                     /gene="relB"
                     /locus_tag="RPM73_004086"
     CDS             723..962
                     /gene="relB"
                     /locus_tag="RPM73_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HDX6522377.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            962..1249
                     /gene="relE"
                     /locus_tag="RPM73_004087"
                     /pseudo
     CDS             962..1249
                     /gene="relE"
                     /locus_tag="RPM73_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
     gene            1321..1476
                     /gene="hokD"
                     /locus_tag="RPM73_004088"
     CDS             1321..1476
                     /gene="hokD"
                     /locus_tag="RPM73_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDX6522378.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1694..1945
                     /locus_tag="RPM73_004089"
     CDS             1694..1945
                     /locus_tag="RPM73_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522379.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            2012..2290
                     /locus_tag="RPM73_004090"
     CDS             2012..2290
                     /locus_tag="RPM73_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522380.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            2292..3341
                     /locus_tag="RPM73_004091"
     CDS             2292..3341
                     /locus_tag="RPM73_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDX6522381.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            3355..4107
                     /locus_tag="RPM73_004092"
     CDS             3355..4107
                     /locus_tag="RPM73_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDX6522382.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(4282..4485)
                     /gene="cspF"
                     /locus_tag="RPM73_004093"
                     /pseudo
     CDS             complement(4282..4485)
                     /gene="cspF"
                     /locus_tag="RPM73_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            4786..5001
                     /gene="cspB"
                     /locus_tag="RPM73_004094"
     CDS             4786..5001
                     /gene="cspB"
                     /locus_tag="RPM73_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDX6522383.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         1430 a         1116 c         1338 g         1405 t
ORIGIN      
        1 tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc ccctcttccg
       61 atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt ttggctattt
      121 ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt ccctgaattg
      181 tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgcacaaa aaaatcttta
      241 atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctag cgggctggcg
      301 cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt ttctgccgca
      361 tgcgggtgtt gcataaaacg tgctacgttc ctttatcgac aggtcaggtc accgctcacc
      421 cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata agttgagttg
      481 tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag atgatggccg
      541 gactctttaa aaaagagctg acctgcacaa tacaggatgg acttagcaat ggctgctcct
      601 ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc ttcgtgactt
      661 gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa gaggtgtaag
      721 acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct tacgccgcgc
      781 ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag tatatcgctg
      841 acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct gaacttgtgg
      901 agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg gatgaactct
      961 gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa agctgggctc
     1021 gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac cccggattga
     1081 agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt cttcaggcta
     1141 tcgccttgta tactaggtta tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa
     1201 aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg
     1261 acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga gaagcaggct
     1321 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1381 acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag
     1441 gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgagt gagaaatccc
     1501 tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg cttcggcggg
     1561 tttttgtttt tattttcaac gcatttgaag ttctggatgg tgccggaata gaatcaaaaa
     1621 tacttaagta gcgcgcaggg ataagaggga tggaccccga acaggggagt gctatttatc
     1681 tggaaggatt ctgatgatga acatcgaaga actgcgtaaa attttttgtg aagatggcct
     1741 ctatgctgtg tgcgttgaaa atggaaatct tgttagtcat taccgcatta tgtgtttgcg
     1801 aaagaatggg gctgcgttaa ttaattttgt ggatgctcgg gtcacggacg gatttatctt
     1861 gcgcgaaggt gagtttgtca cttcattaca ggcattgaaa gagatcggaa taaaagctgg
     1921 cttttctgct ttttcaggag aataaactca tctacaatct tgcgcggggc tgaactcccg
     1981 ctgagtaaca ccgtgccacc ggagaaaacc gatggcacgc aacgcaaaat attacaattc
     2041 tgataattcg cccgttcttg cctgcacgca cgggcggtat tctcacgcat tcaagtctga
     2101 atggttccag caccctccat gcactgcaga acaggccgaa tggctgattc attcttaccg
     2161 caggcgcggg ttcgaggtta agaaagctct cagtctcgac tatcggcact ggataatctc
     2221 tgtcaggctg ccttattccg aacgcccacc acgtgcgtcc cgcactttcc agcaacggat
     2281 ctggaggtaa cgtgcgggta ttacttagac ctgttctggt gcctgagctt gggctggtgg
     2341 tccttaagcc gggccgtgaa tccatacaga tatttcataa tcctcgagtg ctggtggaac
     2401 cggaaccaaa aagcatgcgt aatctgccat ccggagtcgt tcctgccgtt cgccagccgc
     2461 tggcggaaga caaaacattg ctgccgtttt ttagtaacga acgggtgatt cgtgctgctg
     2521 gcggcgttgg cgcattgtcc gactggctat tacgtcatgt tacatcctgc cagtggccta
     2581 atggcgatta ccatcacact gaaacagtca ttcaccgtta tggtaccggc gcaatggtgt
     2641 tgtgctggca ctgcgacaac caactgcgtg accagacatc ggaatcactg gagctgcttg
     2701 ctcaacaaaa tctgacagca tgggtgattg acgtcatccg tcacgcaata agcggtacgc
     2761 aggagcggga attatctttg gctgaattat cctggtgggc ggtctgcaat caggtggtgg
     2821 atgcactacc tgaggctgta tcgcgtcgtt cgctgggatt accagcggaa aaaatctgct
     2881 cggtgtaccg cgaaagcgac atcgtaccgg gagagcagac cgccaccagc atattgaaac
     2941 aacgcacaaa aaatcttgca ccgttgcctt acgcccacca gcaacaaaaa tcaccacagg
     3001 aaaagacggt ggtaagcatc accgttgatc cagagtctcc ggaatctttc atgaagctgc
     3061 ctaaacgtcg ccgctgggtt aaggagaaat acacacgttg ggttaagaca cagccgtgtg
     3121 cttgctgcgg tatgccagcc gacgatccgc atcatctgat tggtcacggg cagggcggaa
     3181 tgggaacaaa agcacatgat ctctttgtgt tgcctttgtg cagaaagcat cacaacgagc
     3241 tgcatacgga tacagtggca tttgaagata agtatggctc ccaactggag ctgatatttc
     3301 gttttatcga tcgcgcgctg gcaattggcg tactggcgta agtggagaac gagcatgaac
     3361 cttgaagcct taccaaaata ttactcccca aaatctccaa aattgagcga tgacgctcca
     3421 gcgacaggca ccggttgttt aacaattacg gatgtaatgg cagcgcaggg gatggtgcag
     3481 tcgaaagcac cacttgggtt ggccttattt ctggcaaaag ttggtgttca ggaccctcag
     3541 tttgcgattg aaggcctgct aaattacgcg atggcactgg ataacccgac attgaacaaa
     3601 ttgagtgaag aaatccggtt acagattatt ccttacctcg tgagttttgc ctttgctgat
     3661 tactccaggt ctgcggcaag taaggctcgc tgtgagcatt gttcaggtac gggattttat
     3721 aatgtattgc gcgaagtggt gaaacactac agacgcgggg aatctgtaat caaggaagaa
     3781 tgggtaaagg aactatgtca gcattgccat ggtaagggcg aagtcagcac agcgtgcaga
     3841 gggtgtaagg gtaaagggat tgttctggat gaaaaaagaa cccggtttca tggcgtaccg
     3901 gtatataaga tttgtgggcg ttgtaatgga aaccggttta gtcgtttacc gaccacgctg
     3961 gcacgacgtc atgtccagaa gctggtacca gacctgaccg attatcagtg gtataagggg
     4021 tatgcggacg tcattggtaa actggtaaca aagtgctggc aggaagaagc atacgcggaa
     4081 gcgcaattga ggaaggtgac gagataaatg atttttgctg aagatggcga catgatgttt
     4141 gcatttttca aaaaacatgg ataagatttt ctcaacgatg ggctttgtgt atccgacgtt
     4201 tagaaaaaag tagaaaaccc gcttataagc gggtttttgt gctttaaatg gggcaataga
     4261 gatattgaat ctcatcccgg gataaacatt ggcagttgaa ggtccatgcg aaccatttat
     4321 ccggcaaaat tccacgcgta atcctgtggt aatttcttct gcatctcgaa gattgagagc
     4381 tgagacatga agctggacat cgatacgacc atcggatggg gtgataagac ccttgccgct
     4441 tttgccgtca aaggttttga caattcctgt cattttacgg gacaaaaaaa ttccttaata
     4501 ctgataactt ggcgcactat acacacgttc ctgaagaaag ctatagtttt ttgatggggt
     4561 tgaagatggc tggatgtcta aaataaacat tgcttcatat gttcaactat gcgttaatga
     4621 ttgcgtcggt ttgaagaaca gacgatatac gaagtagttt actaaagcag ttctcatttc
     4681 aggtgttatt cacttattcc ttctttgagt ctctccaatt aagtacgaag tcgtttctgt
     4741 tatgcaaacc atttatgccg aaaggctcaa gttaaggaat gtagaatgtc aaataaaatg
     4801 actggtttag taaaatggtt taacgctgat aaaggtttcg gctttatttc tcctgttgat
     4861 ggtagtaaag atgtgtttgt gcatttttct gcgattcaga atgataatta tcgaacctta
     4921 tttgaaggtc aaaaggttac cttctctata gagagtggtg ctaaaggtcc tgcagcagca
     4981 aatgtcatca ttactgatta aaattcatcg ctcgtctgta tacgataacg aagaaggctg
     5041 atgcctgagt agagatacgg acagagtagt gaatattgga tctctttaat aaaaagtaag
     5101 gaggtccaat acatgaaaca atggccagca tatttggcaa aatcttaatc aggaaaagta
     5161 tgctaaccat tgtggtgaag tgcaggtttg ctgcatgaat agttttacag cagaagctaa
     5221 ctgctggcat ggcaaaacaa agtgcgtaag tggatgactc ccacaaaaag caccacaatc
     5281 tcaaacccg
//
LOCUS       DAPPNJ010000251         5232 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000251 DAPPNJ010000000
VERSION     DAPPNJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.253"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..426
                     /locus_tag="RPM73_004095"
     CDS             <1..426
                     /locus_tag="RPM73_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522384.1"
                     /translation="INNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            674..1714
                     /gene="pstS"
                     /locus_tag="RPM73_004096"
     CDS             674..1714
                     /gene="pstS"
                     /locus_tag="RPM73_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDX6522385.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1801..2760
                     /gene="pstC"
                     /locus_tag="RPM73_004097"
     CDS             1801..2760
                     /gene="pstC"
                     /locus_tag="RPM73_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDX6522386.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2760..3650
                     /gene="pstA"
                     /locus_tag="RPM73_004098"
     CDS             2760..3650
                     /gene="pstA"
                     /locus_tag="RPM73_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDX6522387.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3741..4514
                     /gene="pstB"
                     /locus_tag="RPM73_004099"
     CDS             3741..4514
                     /gene="pstB"
                     /locus_tag="RPM73_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDX6522388.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4529..>5232
                     /gene="phoU"
                     /locus_tag="RPM73_004100"
     CDS             4529..>5232
                     /gene="phoU"
                     /locus_tag="RPM73_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDX6522389.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLA"
BASE COUNT         1246 a         1286 c         1407 g         1293 t
ORIGIN      
        1 ataaataatt tacagccagt ttattttgat tttaaagaaa taccagctgc agatttttca
       61 tctgttgttg gaagtgcggt aacaacacat aaaattacta aaacggtcac tattgagtgt
      121 gaaaacctgg ggatactgaa taccgatgat atcagtacgt ctttttatgc taccgaaccc
      181 aatacggaca attcaatggt cgtaacttca aactctaacg tggggataaa aatttacgac
      241 aaaaataata aggaaatcaa agtgaacggt ggtgagttgc caacagacat gggtaaatca
      301 acagtgtatg gtgaaaaatc aggtagtgta actttttcag ctgctcctgc gagccttact
      361 ggagctcgcc cagccccggg gcaatttact gcaacagcga cgataactgt tgagattgtg
      421 cgctaatagg tctgacaacg attgttcatc acatcgttgt catctcaccc atttctttat
      481 cacttttaaa tgcatcatga caaaaagatg acatgtggga ttttcatctt cacatttaaa
      541 atcagtaagt tatttggtaa ttcctttctg tcataaaaat gtcatattcc ttacatataa
      601 ctgtcacctg tttgtcctat tttgcttctc gtagccaaca aacaatgctt tatgaatcct
      661 cccaggagac attatgaaag ttatgcgtac caccgtcgca actgttgtcg ccgcgacctt
      721 atcgatgagc gctttctctg tgtttgcaga agcaagcctg acaggtgcag gtgcaacctt
      781 ccctgcgccg gtgtatgcca aatgggctga cacttaccag aaagaaaccg gtaataaagt
      841 taactaccag ggtatcggtt cttccggtgg cgtaaaacag attatcgcta ataccgttga
      901 ttttggtgcc tctgacgcgc cgctgtctga cgaaaaactg gctcaggaag gtctgttcca
      961 gttcccgacc gtgattggcg gcgtggtgct ggcggttaac attccagggc tgaagtctgg
     1021 cgaactggtg ctggatggta aaaccctcgg cgacatctac ctgggcaaaa tcaagaagtg
     1081 ggatgatgaa gccatcgcca aactgaatcc gggtctgaaa ctgccttcac aaaacattgc
     1141 tgtagtacgc cgcgcagatg gctccgggac ttccttcgtc ttcaccagct acctggcgaa
     1201 agtgaacgaa gagtggaaaa acaacgttgg tactggctct accgtaaaat ggccgatcgg
     1261 tctgggcggt aaaggtaacg acggtatcgc cgcgttcgtt cagcgtctgc cgggtgcaat
     1321 tggttatgtt gaatatgctt acgcgaagca gaacaacctg gcgtacacca aactgatctc
     1381 cgctgatggt aaaccggtta gtccgaccga agaaaacttc gctaatgcag caaaaggtgc
     1441 agactggagc aaaaccttcg ctcaggatct gaccaaccag aaaggcgaag atgcatggcc
     1501 tattacctct accacgttca ttctgatcca caaagatcag aagaaaccag aacaaggcac
     1561 agaagtgctg aaattcttcg actgggcgta caaaaccggg gctaaacagg cgaacgacct
     1621 ggattacgcc agcctgccgg atagtgtagt tgaacaggtt cgcgctgcgt ggaagaccaa
     1681 tattaaagac agtagcggta agccgctgta ctaataaaac tccaggccgg gtacggtgtt
     1741 ttacgccgca tccggcatta caaaatgact ttgtaaacgc gtttaactga agagtaactt
     1801 atggctgcaa ccaagcctgc ttttaaccca ccgggtaaaa agggcgacat aattttcagc
     1861 gtgctggtaa aactggcggc gctgattgtg ctattgatgt tgggtggcat tattgtctct
     1921 ctgatcatct cctcctggcc gagcattcag aaatttggtc tggctttcct atggaccaaa
     1981 gagtgggatg caccgaacga tatctacggg gcgctggtgc cgatctacgg tacgttggtg
     2041 acttcgttta tcgcgctgct gatcgccgtc ccggtgagtt tcggtatcgc cctgttcctg
     2101 actgagcttg cgcctggctg gctgaaacgc ccgctgggta tcgccattga gctgctggca
     2161 gccattccaa gtatcgttta cggcatgtgg ggcctgttta tctttgcgcc gctgttcgcc
     2221 gtttactttc aggagccggt cggcaatatc atgtcgaata tcccgattgt tggcgcgctg
     2281 ttctctggcc ccgcatttgg tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg
     2341 attattccgt acattgcggc ggtaatgcgt gatgtgttcg aacaaacccc ggtgatgatg
     2401 aaagagtcgg cctacggtat tggctgcacc acctgggaag ttatctggcg tatcgttctt
     2461 ccgttcacca aaaatggtgt tatcggcggc atcatgctgg ggctgggccg cgcgctcggt
     2521 gaaaccatgg cggtgacctt tatcatcggt aacacctacc agctcgacag cgcctcgctg
     2581 tatatgccgg gcaacagtat cacctctgcg ctggcgaacg aatttgcgga agcggaatcc
     2641 ggtctgcacg ttgccgcact gatggaactg ggcctgatcc tgtttgtgat taccttcatc
     2701 gtcctcgccg catcgaagtt tatgattatg cgcctggcta agaatgaggg ggcacgctaa
     2761 tggctatggt tgaaatgcaa accactgcgg cgctggctga atctcgccgc aaaatgcagg
     2821 cgcgtcgccg cctcaaaaac cgtattgcgc tgacgctctc gatggcgacg atggccttcg
     2881 gcctgttctg gctgatctgg attttaatgt ccaccatcac tcgcggtatc gacggtatgt
     2941 cgctggcgct gttcactgaa atgacgccgc cgcccaatac ggaaggtggt ggtctggcga
     3001 acgccctggc gggtagcggg ctgttaattt tgtgggccac ggtattcggt acgccgctgg
     3061 gcattatggc ggggatttat ctggcggaat atggtcgtaa atcctggctg gcagaagtga
     3121 ttcgcttcat taacgacatt ctgctctccg cgccgtcgat tgtggttggt ctgtttgttt
     3181 acaccatcgt ggtggcgcag atggagcact tctctggctg ggcgggtgta attgcgctgg
     3241 cgctgttgca ggtgccgatt gttatccgca ccaccgagaa catgctgaaa ctggtgccgg
     3301 acagcctgcg tgaagcggct tatgcgctgg gtacaccgaa gtggaaaatg atctccgcga
     3361 ttacgctgaa agcgtcggtg tccgggatca tgaccggtat cctgctggcg attgcccgta
     3421 ttgccggtga aaccgcgccg ctgctgttta ccgcgctctc caaccagttc tggagcacgg
     3481 acatgatgca gccgatcgcc aacctgccgg tgacgatctt taagtttgcg atgagcccgt
     3541 ttgcagaatg gcagcaactg gcctgggccg gggtgttgat tattaccctg tgcgtactgc
     3601 tgctgaacat tctggcgcgc gttgtttttg cgaagaataa acacggttaa cactttgccg
     3661 gatgcggcgt gaacgcctta tccggcctac ggtaagcctg attcgcgaag tgcatcaggc
     3721 acgatgagga aaagattgca atgagtatgg ttgaaactgc cccgagtaaa attcaggttc
     3781 gtaatttgaa cttctactac ggcaaattcc atgccctgaa aaacatcaac ctggatatcg
     3841 ctaaaaacca ggttacggcg tttatcgggc cgtccggctg cggtaaatcg acgctgctgc
     3901 gtaccttcaa caaaatgttt gaactgtacc cggagcagcg tgcggaaggt gaaattctgc
     3961 ttgatggcga caacatcctg accaactctc aggatatcgc cctgctgcgt gcgaaagtgg
     4021 gcatggtgtt ccagaaaccg acgccgttcc cgatgtccat ctacgacaac atcgcctttg
     4081 gcgttcgtct gtttgagaag ctctcccgtg ccgacatgga tgagcgcgtg cagtgggcat
     4141 tgaccaaagc cgcattgtgg aacgaaacca aagataaatt gcaccagagc ggttactctc
     4201 tctctggtgg tcagcaacag cgtctgtgta ttgcgcgtgg tatcgccatt cgcccggaag
     4261 tgctgctgct cgacgaaccg tgttcggcgc tcgaccccat ctctaccggg cgtattgaag
     4321 agctgatcac cgaactgaag caggattaca ccgtggtgat cgtcacccac aacatgcagc
     4381 aggctgcgcg ttgttccgac cacacggcgt ttatgtacct gggcgaattg attgagttca
     4441 gcaacacgga cgatctgttc accaagccag cgaagaaaca aacagaagac tacatcaccg
     4501 gtcgttacgg ttgattcagg agtgcgttat ggacagtctc aatcttaata aacatatttc
     4561 cggccagttc aacgccgaac tggaaagtat ccgcacgcag gtgatgacca tgggcggcat
     4621 ggtggagcag cagctttctg atgcaatcac cgcgatgcat aaccaggaca gcgatctggc
     4681 gaagcgcgtg atcgaaggcg acaagaacgt caacatgatg gaagtggcga tcgatgaagc
     4741 ctgcgtgcgc attattgcca aacgtcagcc gacggcgagc gacctgcgtc tggttatggt
     4801 gatcagtaaa accattgccg agctggagcg tattggtgac gtggcggaca aaatctgccg
     4861 tactgcgctg gagaaattct cccagcagca tcagccgttg ctggtaagtc tggagtcgct
     4921 gggccgtcat accatccaga tgctgcacga cgtgctggac gcgttcgcgc ggatggacat
     4981 tgacgaagcg gtacgtattt atcgtgaaga taaaaaagtc gatcaggaat acgaaggtat
     5041 tgttcgtcaa ctgatgacct acatgatgga agattcgcgt accattccga gcgtacttac
     5101 tgcgctgttc tgcgcgcgtt ctatcgaacg tattggcgac cgctgccaga atatttgtga
     5161 gtttatcttc tactacgtga aggggcagga tttccgtcat gtaggcggtg atgagctgga
     5221 taaactgctg gc
//
LOCUS       DAPPNJ010000252         5220 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000252 DAPPNJ010000000
VERSION     DAPPNJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.254"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..518
                     /locus_tag="RPM73_004101"
     CDS             <1..518
                     /locus_tag="RPM73_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522390.1"
                     /translation="YSLKINQTTPGILVNCDSNNNFACQTAMTEDVIQRITTFFHTSP
                     DVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINS
                     ELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVK
                     KMVDEYQQNSG"
     gene            complement(764..1459)
                     /locus_tag="RPM73_004102"
     CDS             complement(764..1459)
                     /locus_tag="RPM73_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDX6522391.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1452..2879)
                     /locus_tag="RPM73_004103"
     CDS             complement(1452..2879)
                     /locus_tag="RPM73_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDX6522392.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2890..3609)
                     /locus_tag="RPM73_004104"
     CDS             complement(2890..3609)
                     /locus_tag="RPM73_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDX6522393.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4136..4990)
                     /gene="rclR"
                     /locus_tag="RPM73_004105"
     CDS             complement(4136..4990)
                     /gene="rclR"
                     /locus_tag="RPM73_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDX6522394.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1411 a         1352 c         1103 g         1354 t
ORIGIN      
        1 gttacagcct aaaaataaac cagacaacac caggcatact tgttaactgt gatagcaata
       61 ataactttgc ctgtcagacc gcaatgactg aagatgttat acagcgaatt actacatttt
      121 ttcacaccag cccagatgtc aagaacagag aaataaagct ggaatggtca ggagataaga
      181 gagatttacc aactgctgaa gcggaaatat ctcgcgtgca ggcgtctatt atcaaatggt
      241 atgcgtcaga ataccataat ggcaggcaag ttctcgatga gatacaaaca ccttcagcaa
      301 ttaacagtga gctttataca aaaatgatat acctgaccag gaactggtca ttgtatccga
      361 acggtgatgg ctgtgtaact ataagctcac cagaaataaa aaataaatac cctgctgcca
      421 tttgcctggc tctgggattt tttctaagca ttgtaatttc tgtaatgttt tgccttgtca
      481 aaaaaatggt agatgaatac caacaaaact ctgggtagta ataaacagca ttctgataga
      541 gagtgcctgt ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg
      601 atgcctgtct ttttcactgt aaatatcctg gcattactaa tgccaggata tttacagaag
      661 agcagcataa ccatgacgga acggggttaa gtcaggttgt ccttaacgct attttcaacc
      721 tcgtatgcct tcttcaggtt tatgttcaga tctcatatct ctctcagcaa tcctcaataa
      781 tcagatacac cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatatccg
      841 ccgttgaact agggccgcta atgatgttaa tgcaggacgg cattcgttca ccggcctgcg
      901 ctttctgatg caatttttct gcgagttgcg ctacacgcgg caagatagtg cttttacgca
      961 ggataaaaag agaagattcc gggagcaggc tcaatgaacg cccgcgcccg gcggcggaaa
     1021 aaagaaccac gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag
     1081 cctgctcggc ctgcgagata ttctcggcac ctttcgccgg atcccaaaca acggcattgc
     1141 attcctgctg caaacgttca ctaatcccca attcctccag cctcgtgtca ccgctaatca
     1201 cgaccgactg atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct
     1261 cgctggtcag ctcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac
     1321 agcgctgctg ttggttaagt tgggtaagcc gctcgttagc atagttgtta agcggcgcat
     1381 cttctgcttg cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa
     1441 attcgcctcg attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg
     1501 aaactctctc cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca
     1561 aatttgagtg gtgttttgcc gccattgata aaccagcttg ccgcatgagc gccggccatc
     1621 atcccgactt tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt
     1681 tgctctgctt ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt
     1741 tttgacagcg gaatacgcac cggacacacg ctgtcacaag cagtgcataa agagcaggcg
     1801 tagggtaaat ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt
     1861 ggccctggat aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta
     1921 ttcatacaag ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc
     1981 tcagaacgcc cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc
     2041 gcttcgcgcg gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg
     2101 cgcgccagca tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt
     2161 cccatcactg caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg
     2221 gtcaccaggc ataccgaacc tgtctctgcc accgcgaaat tacagccggt aatacctatt
     2281 tcagcactga ggaaatcttc gcggattttt tgccggatga ataaggtcat cgcttcaggc
     2341 gtttccggcc cctcatagcc cagacgttcg tgtagcactc gacggatctg atggcgatct
     2401 ttatgaattg ccgggaccac aacatgagat ggcggatctt gatccagctg gagaatgtat
     2461 tcacccagat cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca
     2521 atctcttcgg tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca
     2581 acctgtaaaa tgtagcgagt ggcgtcttct ttagtttttg caaaatagac gtgaccgccg
     2641 ttttgcgtca ctttttctga gagctggtac agataagcgt cgagattact cagaacatga
     2701 tcacgtatct gggcggcccg atcgcgccac tcctcccagt gccccaattc atcgaccatt
     2761 ttttgccgat ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg catgatcgga
     2821 tcttcaattt gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc
     2881 tttatatcct cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc
     2941 ttcccgttgt aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata
     3001 ctcagggcgg acttccatca ggtgcacaac cttttctttc accatctcgc cggatatttc
     3061 ggccattttg accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt
     3121 caacagctcc agtccacgca catttttcag cagcgtaagt ggctcgtcct tcactcccag
     3181 cttacgggcc aggctacaag atgggtgata cacagctctc ccttgcaaac tggcacctac
     3241 atcgactacc cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac
     3301 cttttcggca cgtgatgccc attcaggttc atccgccaga tacgtcgggt aactttttac
     3361 ggcataggtg caagagccag ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc
     3421 gatcagattt ttcatccctg gaatcgcttc tttgatataa ccgctattga tcgcaggctg
     3481 accgcagcat ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag
     3541 cacggagtct cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt
     3601 gacattcaca actaatactc cattacttca tgcccattta tgcccgatgt ttccccccca
     3661 ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac
     3721 ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt
     3781 tatttctttc ggcatcgctc acattttatg gggaaatcga cgttcatttt tggcataaga
     3841 aatccattta aaaaggctac atcccttata cctattcaga gctacttatt caggggttat
     3901 tgagatatac actgatggtg tacaaaaaaa tcaccgacaa taatcacgta tcaagtaata
     3961 gtcggggttt tatttaattg aagcaattta gtttgcattt ttatttaaaa actgagctag
     4021 agtccatcag aaaaaaatat ataaagccca aaccaccgtt ggaaatagaa atagttgaaa
     4081 ggctcacaac tcccggaatg cggattatct ttgccctgat ttctggcggt tttattcagg
     4141 gtgcaagctg tctgaccctt tcccgatatt ctcccggagt acaaccaaac tcgcggacaa
     4201 acgccttgtg aaaagatgat tcactggcat agcctactga ctcagcgatc accacaacag
     4261 ggagcatttc ccgggaaaac atctgggccg ctatttgtag acgcaacttt gttaatacag
     4321 ccagcggcgt ggttccggaa acatcacgga aaagctgggc aaaacttgcc cgggacatgt
     4381 gagcgatgct ggccagcgat tcgacggtcc aggcgtgccc tggcatttcc agcatttgct
     4441 gtattaccgc accaagacgt ggatgcagaa gcaaactgag aatgtttttc tcagtattaa
     4501 cctgtgcaat ccactcacgc accgcgaggg taaagaatgt agcgcagatc tggctacaca
     4561 gtgcatccac tcccggcatc gccgatctgg attcctgttg taaaaacggg atcgcctccc
     4621 tcagccagtt atattccacg ctgtggttaa ccggtgctaa aaacagcgtt tccggcaaag
     4681 acgttaaaaa ataacgcgcc gaatgttgca accgaagagt gccgcagaca atacaggtcg
     4741 attcattatc gacatgactc aggcgatgag ctgaattttg tggtagcagg accacatttc
     4801 ccgggcgtaa tgtaaaaatc tctcccgtcg gcatttccag cttcgccgct ccttgcgtta
     4861 acgcatgcca acgaataacc gataattccc cggcaccatg tggaagctgc cagtcacttc
     4921 ctaacacgca attcttatcg atcgttcctt gtggagcgtt aagcatcaac aaacggctaa
     4981 gggcatccat atcagactcc tgagtggttt ttgattacaa acaccaacaa tcttagtaaa
     5041 taactaatct aataccatca aatggttaat catgattttt gttttgtgat tctgattaag
     5101 aaaaagaaaa taccagacga taagacaaaa tatcaagcgt tgtgaagaaa tggtatttgc
     5161 tcttttgcgt ctatagtcat gatgtcaaat gaacgcgttt cgacaggaaa tcatcatgaa
//
LOCUS       DAPPNJ010000253         5194 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000253 DAPPNJ010000000
VERSION     DAPPNJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.255"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            241..2946
                     /gene="malT"
                     /locus_tag="RPM73_004106"
     CDS             241..2946
                     /gene="malT"
                     /locus_tag="RPM73_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDX6522395.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2989..4005)
                     /gene="rtcA"
                     /locus_tag="RPM73_004107"
     CDS             complement(2989..4005)
                     /gene="rtcA"
                     /locus_tag="RPM73_004107"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDX6522396.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALRLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLLFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4009..>5194)
                     /gene="rtcB"
                     /locus_tag="RPM73_004108"
     CDS             complement(4009..>5194)
                     /gene="rtcB"
                     /locus_tag="RPM73_004108"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDX6522397.1"
                     /translation="MWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIG
                     SVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRC
                     KRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICL
                     DESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDY
                     LKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHF
                     GEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKA
                     KKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCV
                     KG"
BASE COUNT         1229 a         1478 c         1310 g         1177 t
ORIGIN      
        1 gaagaaaccc ttgcttaaat gaatctgatg aacataaggg aaaccagtat tcacgctgga
       61 tcagcgtcgt tttaggtgag ttgttaataa agatttggaa ttgtgacaca gtgcaaattc
      121 agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca
      181 ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact
      241 atgctgattc cgtcaaaact aagtcgtccg gttcgactcg accataccgt ggttcgtgag
      301 cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacgagtcct
      361 gcgggctacg gaaagaccac cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc
      421 tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc
      481 gccgtgcagc aggcaaccaa cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg
      541 caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat
      601 agcccacttt atctggtcat cgatgactat catctgatca ctaatccagt gatccacgag
      661 tcaatgcgct tctttattcg ccatcaacca gaaaatctca ccctggtggt gttgtcacgc
      721 aaccttccgc aactgggcat tgccaatctg cgtgttcgtg atcaactgct ggaaattggc
      781 agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca
      841 tcgccgattg aagctgcaga aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg
      901 gcactacagc taatcgccct ctccgcccgg cagaataccc actcagccca taagtcggca
      961 cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg
     1021 gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg
     1081 aacgatgccc tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag
     1141 attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg gttctgctat
     1201 cacccgctgt ttggcaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg
     1261 ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc cagcgaagcg
     1321 attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac
     1381 gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg
     1441 ccgtgggaca gtttgctgga aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa
     1501 agccaacatc gctatggcga agttaacacc ctgctagccc gtgctgaaca tgaaatcaag
     1561 gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt
     1621 aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga agagctgccg
     1681 ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc
     1741 aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag
     1801 cacgacgtct ggcactacgc cttatggagt ttaatccagc aaagtgaaat tctgtttgcc
     1861 caagggttcc tgcaaaccgc gtgggaaaca caggaaaaag cgttccagct gatcaacgag
     1921 cagcatctgg aacagctgcc gatgcatgag tttctggtgc gcatccgagc acaactgtta
     1981 tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg
     2041 tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg
     2101 gcccgtggtg atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg
     2161 aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg
     2221 caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag
     2281 tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg
     2341 ctgggcgagt ttgaacctgc ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt
     2401 ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact gtactggcag
     2461 gccggacgta aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc
     2521 accggattta tcagccattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag
     2581 ctgattcaac tcaatacgct gccggaactg gaacagcatc gcgcacagcg tattctgcga
     2641 gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt
     2701 ctgctaaatc atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa
     2761 tggcaggtcc tggggctgat ttactccggt tacagcaatg aacaaattgc gggcgaactg
     2821 gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc
     2881 gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg
     2941 gtgtgagttt agccggataa tgcattggat ccggtttaca tctttacatc attcaatact
     3001 cacccgcgtt acgccatctg tttctatcaa accaaaccgc accggcaaga aacgctccac
     3061 caccgcgata ttggtcagca gatggcatga aggatgggcg accttaaatt ctcccgcgcc
     3121 cgccagcgcc atcggcagta ccagctggtc ggcgagatat tcccccactg ccgccgggct
     3181 tgccaggtag cgtttcacct ctttcactaa ctgcgcggct accacctcgg cactgacgcg
     3241 cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgagac
     3301 ggtattaccc ggcccctggt cacgcggcag gttatgaata ttctgctcat gcagggaaaa
     3361 actacccgcc agcgtagcga tttcacgctc agcgacatgg cgcggcacgc cagctaataa
     3421 cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacga
     3481 tgccaccggc gagacttccg ttgccaccac gccgcctccg gcaggataaa aaccgtgacg
     3541 taacagcgtg gtttgctgat gaattcctat tttcgccagc agcggctcca gcacccggcg
     3601 gataaaatcc gcaggcgggg ccgacgggtt atcggtgcct ccgctcactt caacacgcga
     3661 aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacagcttcc
     3721 ggcactaccg atagcaaagc ggtactcgcc gccgcgcacg gtgccaggcc ggaagagcag
     3781 acgctgcgac cccagctccg cgccttccac ggttgctccg caaatttccg tcgccgcctt
     3841 taccgcggtc agatgctggc gcaacagccc cggtttcgcc cgcccggcac gaatgctggt
     3901 gatggtaaat ggctggccgg ttatcatcga cagtctcagc gccgagcgca ggatctgccc
     3961 gccaccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac
     4021 gcacaccacc tgacgcaggg tatagataac ttccaccaga tcgctttgtg ccgccatcac
     4081 cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca
     4141 ttccacatgc gcggtggcac gaatttgatc ttccacgctg aacagttttt tcgctttagt
     4201 tcggctcatt acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag
     4261 cccacggacg ataaagcttt ttgctcccat cgaaccggga ataattccat attgaccagc
     4321 acgcgcagac accgcgcctt tacgcgtcac gtagatctct tcaccaaagt gctgttcttt
     4381 ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg gctgtctgac
     4441 cgttttctgc gtaatgctct gcaatgccgt taccacgttt tccatcatcg catcgcggtt
     4501 aaggctggca aaaagctgcg cccaggccac ggctttcagg taatcatcaa agtattccgt
     4561 accttccata aagtacgcca gatcacgcga cggcaacgtc tcaagcgttt cctgcatctc
     4621 tttttgtgcc agatcgataa agtaagtccc gatggcgtta ccaattccgc gtgaaccgga
     4681 gtgcagcata atccacacct ggtccgactc atcaaggcag atttcaataa agtggttacc
     4741 ggttcccagc gttcccaggt gtttatagtt attggtattc aggaaacggg gatatttttg
     4801 cgttaaccac tgataaccgg cttcaagctc agcccattta gcatcgacgt taacaggtgg
     4861 attttcccag gcacctttat cacgtttaca acggccagtg gtacgcccgt gcggcacggc
     4921 cgtttcaatc gcctgacgca gctctgccag gttttcaggc aggtcttccg ccgttaacgc
     4981 ggtacgcagc gcgttcattc cacagccaat atccacgccc accgccgccg gaataatcgc
     5041 ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta catcaggcat
     5101 taccgcaata tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc
     5161 atcggcctct accggcacgc ctttggtcca catt
//
LOCUS       DAPPNJ010000254         5025 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000254 DAPPNJ010000000
VERSION     DAPPNJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.256"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            92..867
                     /locus_tag="RPM73_004109"
                     /pseudo
     CDS             92..867
                     /locus_tag="RPM73_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1000..1574)
                     /locus_tag="RPM73_004110"
                     /pseudo
     CDS             complement(1000..1574)
                     /locus_tag="RPM73_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1628..2506)
                     /locus_tag="RPM73_004111"
     CDS             complement(1628..2506)
                     /locus_tag="RPM73_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDX6522398.1"
                     /translation="MKLKMQDLRLFNIVFESDPGWILDFSNRTLSAFFDEELNIDIDD
                     ERYQKEGTSKAKRVRCLLKQVDRETALRVLGALWQYKTESMPEQAEQSRNDYLALISR
                     LENADTDEAKGVKPVQAWHGVDWHSLIAEMNEMKSLPPHPRGFRFEAWLAELFSIFKL
                     APRSSFRNTGEQIDGSFRLNDEFYLMEAKWHQKRTSAADLHVFEGKLSTKATWTRGVF
                     ISWMGFTPEGLTAFGKGKRVICVSGYDLYHSLNHRIPLPDLLDAKTRHAAETGEPYAE
                     FDRLYALKDKQFGTLK"
     gene            complement(2834..3565)
                     /locus_tag="RPM73_004112"
     CDS             complement(2834..3565)
                     /locus_tag="RPM73_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDX6522399.1"
                     /translation="MNQPIHNAYWLSRFESILNGALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRKKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPVPGGCSDTRKDSDKKSGGSAVLWVKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGLGRLLDGR"
BASE COUNT         1482 a         1181 c         1103 g         1259 t
ORIGIN      
        1 taggctagcc ccctgaatct ccagtcaacc aatatcactt atttaagtga taatcttaat
       61 attagttttt agactggtca ttggaaaaca gatgattgat gcctcaggac cggagaaact
      121 cagacggcat actgcacagg aaaagatcgc taatgttcag aagagctttg aaccaggaat
      181 gacggtctcc ctcgttgccc ggcaacatgg tgtagcagcc agttacttct ctggcgcaag
      241 caataccagg agggaagtct tactgcggtg actgctgggg aacagatcgt tcctgcctct
      301 gaacttgctg ctgccataag gcagattaaa gaacgccagc gcctgcccgg aacaaaacga
      361 tggaaaatga actcattaaa gaagccgttg aatatggacg tgcaaaaagt ggatagcgca
      421 cgctccctta ttgccggggt gatgcactgg gcggtcacta ccggcggctt caacagtgaa
      481 acagtacagg acgtcatgcc aggagcggtg gaacgccgct tcggcaacga gcttccggcg
      541 tctccagtgg agtggctgac agataatggt tcatgctacc gggctaatga aacacgccag
      601 ttcgctcgga tgttgggact tgaatcgaag aacacggcgg tgcggagtcc ggagagtaac
      661 agaatagcag agagcttcgt gaaaacgata aagcgtgact acatcgtgtt catgcccaag
      721 ccagatgtta atgacggcat tgcataacca tgcagtagca atcgaacatt acaatgcagc
      781 atagttctaa gttatcgctc gccgcaggta tatatgcggc agcggatcag taatgggtta
      841 aatgataaca gatgtctgga agtataggga gaaattccct gagggattcc caaaaaattt
      901 agtaaaccaa tatcatattg gtataccgtt gttgtaaaat gttcagccct gcgtcaagtg
      961 acaaggtgtt tagtatgagt ggggaatgtc ggaacctgtt taggattctg tgtaaatgcc
     1021 ttttttcaga agtgacaggc agtcaccgaa ctcgataata aagcggctca ttgccataca
     1081 cctgtccttc aacgacaccg tccatttctg ggacgcagac tggattgcca gccacaccac
     1141 ttttttcacc gagtcgtctg tcgggaacac tttacgcttt ttgatcgcat ggcggatcac
     1201 actgtttagt gactcgatgg cattcgtcgt atagcgcact ttgcgaatgt ccgtcagata
     1261 agcgaagaac gtggtaagat tcggtcagtt agcctgccag ctccgactta tctagtgctt
     1321 gctagcctgt ctcttccgtg ggtgcctgat taaatctctt tcaggtcgcg agtgacggct
     1381 ttgtagtcct tccatgacac gaagcgcagg ctgttgcgca ccatatgcac gatgcataac
     1441 tggatgctgg cctccggata tactgtgttg atggcatctg gaaagccttt caggctatct
     1501 acacaggtga tgaggatatc gttcagaccg cggtttttca gttcagtcag cacattgagc
     1561 cagaacttcg accctgtaca cgattctgtg tgaaacaacg gggagcaggt catcgcttcc
     1621 caacccgtta tttcagcgtg ccgaactgtt tgtctttcag tgcatatagt cggtcgaact
     1681 cagcataggg ctctcccgtt tcagccgcgt gccgggtttt agcatccaga aggtcgggta
     1741 aagggatcct atgattcagt gagtggtaaa gatcataacc actgacacag ataacccgct
     1801 tccctttacc aaaagccgtc aggccttcag gcgtaaagcc catccaactg ataaaaacac
     1861 cgcgtgtcca ggttgccttg gtactgagct ttccttcaaa tacatggagg tcggcagccg
     1921 acgttctttt ctgatgccac tttgcctcca tcaggtaaaa ttcatcattc agtcggaaac
     1981 ttccgtcaat ctgttcgcct gtatttcgga aagacgaacg gggagcaagc ttaaagatac
     2041 tgaacagttc agcaagccag gcttcaaacc gaaaaccacg tggatgtggc ggcagagatt
     2101 tcatctcgtt catttcagcg ataagtgaat gccagtcaac accatgccat gcctggacag
     2161 gcttaacgcc tttggcttca tcggtgtcag cgttttctaa gcgggaaatc agtgcaagat
     2221 agtcattacg gctttgttca gcctgttcag gcatggattc cgttttatac tgccagagcg
     2281 caccaagaac ccgcagagcg gtctcacgat caacctgttt cagcaagcat ctaactcgtt
     2341 tggcttttga cgtcccctct ttctgataac gttcatcatc aatatcaatg ttcaattctt
     2401 catcgaaaaa agcagacaag gtgcggttag agaaatccag tatccagcca ggatcgctct
     2461 cgaacacaat attaaacagc ctcaaatcct gcattttcag tttcacgcct acctcctttt
     2521 ttcacagtat aacgggatga aagtggggga ggaaatgagt cagaacctgt acaccagcat
     2581 taggggattc cgcgtctggt acaaagcaga ctatgagaag agagacgcta taacatttga
     2641 gtcctaccaa tcgatctgtt catttctatg aatggcagta aatgcaaata accagaacaa
     2701 ccaaaggttt ttcgacttat gtgtcacgaa cggaagtaga tggcaatcca ttgcattact
     2761 tcaggacgac ttttcagaaa gcgagctaag ggcgttggca taaactcagg catcggatgc
     2821 tgttttacag ggttcacctg ccatccagta gacgccccag cccacgactg caaccataat
     2881 tacgttgacc agaaccgtca tgctgcttcg tctcatactt cacgagataa cgggctctct
     2941 ccatggcttt ctgcatcgct tccacatcac cacgcttgac ccagagaaca gcggatccac
     3001 cagatttttt gtcagaatcc ttccgggtat cagaacatcc accaggcaca ggcgtatcat
     3061 cggaagaagg gcgagcatca gatgatgtcg gtttacggaa aggcgtccgc ctggaaaaat
     3121 gaaccagtcc attaccctgc cagggctcaa gatgcagagc actgcaccat gccagttgga
     3181 tcagcgtcgc cagcgaagaa ggaacggtga aatcccctgg cgagcaccag gtatctttgt
     3241 tgagcagtaa tatcacgtga taatgtttcc tgcctttttc cttgccaaac tcccgacacc
     3301 agaaataacg cagagtggtt gcacgcacac gcttgttggc acgtcgtttt ttccgctggt
     3361 aagcctgaat ttttgctttc agggatgcaa aaaaacgaga aatcaccgct gaatctaaat
     3421 cggtatccat gatggtggca ggcatatact cagggaaacg taaatccacc cggattaacg
     3481 agacggcacg gtgttgcgcc agggcaccgt tgagaatact ttcaaaacgg gataaccagt
     3541 aagcattgtg tattggttga ttcatgatga gagatctccg ttgatttgga ttgacttcac
     3601 atcattaatc accgcggtaa aactttacgg catccggcat tgagcaatgc tacaactttc
     3661 tgattaagag tgattgaaac ggaacaaacc atcccggaac aaaaagataa tcctacacac
     3721 agggtaaaaa cactcaagaa cagcgaatgg atttaaaaac gacatggaca acgagaaaag
     3781 tcagaaggga aaaggatcca cagaacattc aaacaaaaca ccagaaacgc aaccagaacg
     3841 cttcctggta atgcaaatca acaacaacgc caaaacctac agcttaatca cacaacttaa
     3901 tcacggatgc gaatgacatg tttaaaacct gtttacagat aacgccgtta cacgatcatc
     3961 cccatcagaa tcgattttcc cttcctgaaa gcgttacgtt tcactacgca ccataaaaac
     4021 gattaaccat cgataacgta aacttcatct gaatcaaaac accaaaaccg ttcatcacac
     4081 tttcgcaatg caagaagagt cacatcatga cgaaaacaat gaccttacac ggaaaagaaa
     4141 aacgtcagga atcgttaatg cctaaatctg attgttatca aaacatccag aaagtcgttt
     4201 aacagaatcg tcgtaagcac atcgaaaaga acgtcagatt acttcaggaa agttcgggtt
     4261 gatttagcgt aaagatactt ttctcatgct aacgtcgaaa ctcgaaatat caggaaaggt
     4321 taaacgtaac atcagtaaaa tcgaatagca gattgattgc tgaagcagaa aggttttaca
     4381 gcagcattaa actgatttag ttaacgtaaa gatttactaa actggatgtg tatagcaatg
     4441 cttaacgaaa acacttgtag aagtgtttat gaaaatacgt gtataacact tactgaaatg
     4501 cttagtaaaa atacgtgtat atagtatcat accgacattc tcaactcttc caaacatcca
     4561 tgcagactga atgttgatta cccgttttca catcaggcat ggttctttac tgaccaaaaa
     4621 acaaactggt ttactcttga attattttct ctacctcaga aaacatggtc tcaggaatct
     4681 gttatggctt cgtagtttct gttgttcttt tcataaaagt tataagttat tgtcataatc
     4741 atacgataat cactttatgt tagtttttac tgtcacacgc tactgacttt ttcagtcatt
     4801 gccagaaagg ggctcttttt acttccattt caccactcaa caaatgcaaa actcgtccag
     4861 taaagcggac caccacttgc tcacaagcaa cgaatcgtct ccaggaacag ccctcaaaca
     4921 ttgcagaaac aaaaacaaca aaaccctcat tatcactgat gttcggaaaa tttccatgac
     4981 ccaaaaaacg aaatttaaat gagacgcatt atcatgcgtg tgatc
//
LOCUS       DAPPNJ010000255         4991 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000255 DAPPNJ010000000
VERSION     DAPPNJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.257"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(165..502)
                     /locus_tag="RPM73_004113"
                     /pseudo
     CDS             complement(165..502)
                     /locus_tag="RPM73_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604131.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
     gene            complement(480..839)
                     /locus_tag="RPM73_004114"
     CDS             complement(480..839)
                     /locus_tag="RPM73_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HDX6522400.1"
                     /translation="MTLLLNDKQYNELCEAAEGRNLGAVFSYSEPEEPPPLNFSFEER
                     KKVFLWVLTRLLKEGRIKLVKHGKFLEGSVEEQVERFRQAFPKTEEEMEDGIWFFDES
                     CPGGAVWGLEDGSLEWT"
     gene            988..2523
                     /locus_tag="RPM73_004115"
                     /pseudo
     CDS             988..2523
                     /locus_tag="RPM73_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2569..2793)
                     /locus_tag="RPM73_004116"
     CDS             complement(2569..2793)
                     /locus_tag="RPM73_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HDX6522401.1"
                     /translation="MFESQNLTDKQIHNYAQQLAGDTPLKEVRPGIYTAKLDNGTSIT
                     LRNLSSSQEQTGARWTIDIKVRITVKEIGE"
     gene            complement(2892..3263)
                     /locus_tag="RPM73_004117"
     CDS             complement(2892..3263)
                     /locus_tag="RPM73_004117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522402.1"
                     /translation="MLNVIATETAANTAAAIAGATGNASKDTGNDNSWDTGGGCEGSR
                     EQCAVRDPSRDGTRGPGHVLNGDNTDEDKQPNQGKELAGATDNEKAELGSAGAGTPGD
                     GDQIMKKKPVIRRFSNNDLMN"
     gene            complement(3257..4181)
                     /locus_tag="RPM73_004118"
                     /pseudo
     CDS             complement(3257..4181)
                     /locus_tag="RPM73_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531944.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            4189..4332
                     /locus_tag="RPM73_004119"
                     /pseudo
     CDS             4189..4332
                     /locus_tag="RPM73_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1243 a         1326 c         1101 g         1321 t
ORIGIN      
        1 cgcggcaatt actctgtcct ttttgtaatc cgagtattat atgtataatt tcatacaaag
       61 tatgggtgtg tattatcgtg aaggagatcg gtggggataa cctccattga aattggagtt
      121 actcgccgat cactttaaca ataatacgca tctgtggtat cattccccac cacctcgccg
      181 gaggcaacac ctgcagcacc ggtaagggta ttatttccct gtgccaccgc cagtacagca
      241 ttcaccacgg catgggctac cacacgcccc gcaccgtccg ggggtctgtt tgataatttc
      301 agcaatatac ggcgcagcac catctgccaa ggctgcactc agattaccgc cagccagtcc
      361 ctgtaccgct gccgttgcag cctgtatcgc ctgctggtat tgccccccat cccaaaaccg
      421 gagccgccat attgttgtta tacgctcgct gcgccgcatc ctgcgctgtg aagggtttat
      481 caggtccatt cgagagagcc atcttcaagc ccccaaacag ctccccccgg acaactctca
      541 tcaaaaaacc agatgccgtc ttccatctct tcttctgttt tcggaaatgc ctgtcggaaa
      601 cgctcaacct gctcttcaac acttccttcc agaaacttac cgtgcttgac gagtttgata
      661 cgcccttctt tcagaagacg ggtcaatacc cataaaaaaa cttttttcct ttcttcaaat
      721 gaaaaattta acggcggggg ttcttctggt tcactatagg aaaaaacagc ccccaaatta
      781 cgaccttctg cagcttcaca taattcatta tattgcttgt cattaagtaa taatgtcatt
      841 taaattttat ctccacatct ttgtatttat atgcaatgtc agaaagttgt ttattcccct
      901 taatgcgtat tttcatgaaa ggagatcact caacaacccc ttgtgtcagc atagttgtcg
      961 cctagatttt tacacaggag agagactatg cccgcacatg cagtaccaat tgatataaaa
     1021 gaacagggcg ttacctggtt attaccacct tatagttggt ccatacggca tattgctcgt
     1081 aaactagacc tgagcccttc agtaatagca agatggagac ttgaactggt gcaacaaggc
     1141 ctgttacctg aaaatgaaaa aatatcttct gtcgatggaa atgaatggac accggagcga
     1201 cgattttctg ttgtaattga aactgcaaca ttatctgaaa tggaactggc tgattattgt
     1261 cggagaatgg ggttatttgt cgagcaagtt aaagaatgga gagctatatc cattcaggct
     1321 catgaaatga aaatggcaga aagtcgccgt gctgataaag aattacgcaa agcaaagacc
     1381 cggttacggg aactggaaaa agaacttctc cgtaaagata aagctctggc ggaagcaact
     1441 gctcttttga tgttaaggga aaagttcaat gccctctggg acaacagcga ggaaggctga
     1501 ctcctcttcc agagcggcag aagattatta atctggtcag cgaggctgtc agttcaggag
     1561 cgagaaaaac acaagcctgc cagtgtattg gtatttcagt cagaacactt ccacgttgga
     1621 tgcagggtgg tgtggttcat gcagatcaga gaccaggtgc atcaaggcgt accccgggga
     1681 ataaactgtc agaaacagaa agattaagaa tacttgatgt atgcaattct ccggagtttg
     1741 catcattgtc tccgaatatt attgttccga ctcttgccga cagaggggtt tatatcgctt
     1801 cagaatcaac tttttatcgc gtattaaaat cagctaacca actgacaagg agaacccgtg
     1861 aacaccaata taagcggcct gaagtactca aagctgtagc ccccaaccag gtgtggatct
     1921 gggatattag ttatatccca tcatgggtta aggggcagca tttttatctc tatatgatcg
     1981 tcgatattta tagccgaaaa attatagctg ccgaagtatt tacgaaagaa tctgggcagt
     2041 atgcggcaga actgcttcaa cgtgctgtat ggaatgaaaa atgttcatcc cgcaatctcg
     2101 tgcttcattc tgataacgga ggaccaatgc gcagttgtac gttactggca aaaatgtacg
     2161 ctcttggcgt attaagttct tattcccgtc ccctggtcag caatgataac ccttattccg
     2221 aatcgctctt ccggacgatt aagtattgtc catggtggcc agagaatggt tttcgaacca
     2281 tagatgaagc cagaagttgg ttaagcaggt tcacgcgatg gtacaacctg gaacacaagc
     2341 cagcggtatt aagtatgtta ctccggatga acgccatcgc ggaattgatg cacaaatact
     2401 ggaagccaga aaaaagttta tcgcgaagct cgcaaacgtc atcctgagcg ctggagcaaa
     2461 cagttgcgtg actgggaact cattcaggca gtttatctga atccagaaaa agaagcggca
     2521 taactgtaat tttattacat caggcgacaa ctaccttgaa aaacgccgtt actcaccgat
     2581 ctccttcacg gtaatacgca ccttaatatc aatagtccat ctggcacctg tttgttcttg
     2641 agacgacgat aaatttcgca acgtgatcga tgttccgtta tccagctttg cagtatagat
     2701 ccccggccga acctctttca gcggtgtatc gcctgccagt tgctgtgcgt aattgtggat
     2761 ctgtttatcc gtcaaattct ggctttcaaa cactttcgtc gtaccataac ggttacttac
     2821 caaactctgc ctgatagtct gcccatccac cgttaactct ccaaccgggt gacttttatc
     2881 atatatttta ctcagttcat caaatcgttg ttgctgaacc tcctgatgac gggctttttc
     2941 ttcattatct ggtccccatc ccccggtgtt ccagcacctg cactaccaag ctcggctttc
     3001 tcattgtccg ttgcgccagc cagttcttta ccctggttag gctgtttatc ttcgtctgta
     3061 ttgtcgccat tcagcacatg ccccggtccc cgggtgccat cgcgactcgg atcacgtaca
     3121 gcacactgtt cccgtgagcc ttcacaacca ccaccggtat cccagctatt atcattcccg
     3181 gtatccttac tggcattacc tgtagcacca gctattgctg ctgccgtatt agctgctgtt
     3241 tcagtggcta tcacgttcag catattgttt tcagccgagt tcctacctgc ctgtgcaccc
     3301 gtaccggcag cacttgtact gttgccggca agcccgcccg caatacctga ggcaatggtg
     3361 accagcatgc tgatttgctg acgttcctgt tcactcaggt tatcggtaca accaccgtag
     3421 tagttatcaa tgatataccg ggcagcaagt tcaccactga atgcccctgc agcaccggaa
     3481 gccgcattgt ttccgcccag ttgtgcagca agcgctcccc agactgcgtg agccatcagg
     3541 tttgcttccc ggttgtcacc ggtagcctgt tttataagct gtgccactgc tggattcaga
     3601 ccgccagcca gtacctgccc cggattccct gcattcagtc cgcccagcac gcccgtgatg
     3661 gcctgtacca ccatctgcgg cgtactgccc gtaccgtagc ctttcatgac ctctctgtat
     3721 tccggggtct cccgtaactg tgcatcactc agccctggat atttcgccct ggctgctttc
     3781 atcgcttcag tttcgccata cgtcatgacg atgttactca tctgaccgct aatctggctg
     3841 ataatctgcg cttcctgcag acgcttctgc tctttctccc tgtcaaatat cggtgcaatg
     3901 ctgccgttgg cgttttccgg gtcccggctg agcccggcaa tattctgctt ctgactctcc
     3961 ttatcacgga taatgagatt tctgccgctg atggcggatg acgtggtacc ttcagcatgt
     4021 ccactgctgc cggacatacc ggtaagtgcg ttcgccgcac cggctattgc atttgaggcc
     4081 accatctggc tggctgacat ccctgagcca cccgataaac tgattctggt atggctggct
     4141 ttatagtctg cctggttatg gatatccgac cagcccagcg tgttggtact aatgagcttg
     4201 ctggaaaccg cgaaacgcaa cggtctggag ccgcatgcct ggctgacaga cgtcctgatg
     4261 cgtctgccgg agtggccgga ggagcgactg gcagagttgc tgcctcttga gggatttacc
     4321 ttctccgggt gagtgatacc tgccgtcagg tgttcgtgca ccgggccata acctgcagtc
     4381 gggagttgaa ctcctgacga caggaaatga gccagaccag caaccacacg ggctgacagc
     4441 gaataagaac ctgaatcagc ccggagtcat tcccgggtca gtacatcatc acctgccatt
     4501 aatccccgag tcagtacttc gcggcctgac gcgatcaccc tactcagtac ttcagagccc
     4561 gcctccggta cctgcctggc gatcgcccct gtgcaacaaa ataaatcaac cgttatcaga
     4621 ccacgatcgt tcgtatgcgc cgccgatgac gcgcgcctga aaaacgcgtc aatatgagtt
     4681 ccccggaagc ttaccccagg tcaattgccg tccattttgc ttctgtctga gccttacagg
     4741 cagcagtctg acatttagcc agctcatcgg ctttttgagc tttctgggct ttactcagat
     4801 agttattctc aacaacaacc acaggggatt agaagccttt tagcctgaaa tattttgcga
     4861 gagcacatcc aataccaata aatgagccaa tcacacatcc gataaacaaa acatgccgaa
     4921 tctctttcaa actaatattt aaattacctg ttatcaacca ctccaccaaa gaaaaaaaca
     4981 catcaataca t
//
LOCUS       DAPPNJ010000256         4897 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000256 DAPPNJ010000000
VERSION     DAPPNJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.258"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..742)
                     /locus_tag="RPM73_004120"
     CDS             complement(<1..742)
                     /locus_tag="RPM73_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDX6522403.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPA"
     gene            complement(799..3171)
                     /locus_tag="RPM73_004121"
     CDS             complement(799..3171)
                     /locus_tag="RPM73_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDX6522404.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYNKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(3209..4894)
                     /locus_tag="RPM73_004122"
     CDS             complement(3209..4894)
                     /locus_tag="RPM73_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDX6522405.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1303 a         1112 c         1097 g         1385 t
ORIGIN      
        1 tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta ctgtcgatat
       61 cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa aattggcgca
      121 attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca cggtctaaat
      181 tacgggtatt agccagcagg aaagggcggc gcgcctgaga ggtgcgccat ttcgcatcct
      241 gatgggcacg ccattcctca gtaattacgc cacgttcagc gtctacttcg agtttttcaa
      301 aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga ttttgtttct
      361 gcgtagtcgg caaactcacc tgatacaccg tttcgtcata gctggtatag gcattaacat
      421 cgcgaccaaa acgcaggccc attgactcaa atgtttcgat gactttatta cccggccatg
      481 tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc tcattgtctt
      541 cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc tttggatgag
      601 catgcggata aatcatatat cgcaagccat tgcccagttg cccggtaatt aacttttcat
      661 cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc actaacgtca
      721 gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa tttacccggt
      781 taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag tacggccact
      841 ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg gttttgctat ccagtacatt
      901 gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg tccaggacaa
      961 gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct tgtaatctga
     1021 atattcgctg atgtattgcg catttgtctt accaaggata atgcgagctt tacgcgcttc
     1081 ttgccaggcc agcgtattcg cccacactaa cccgctcggt tgatgcgtga aatccatgtt
     1141 taaggagatc tttaatgggt tattaaaatc tgccactgga acgctatcgt aagagaccag
     1201 attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac cattgtttaa
     1261 cgacaagttg cccttgctct tgttatagct aaagacaatt tgtgggttaa tatctacctg
     1321 gcggatatgc aggggttcgg ccagttcaaa actgaggctg aacgaatgcg ttttggtttt
     1381 gccatcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac gactgctttt
     1441 gctgatttga tcatgcgctt cacggtaaac atagtttgcg cgtgcaataa cgttcttacc
     1501 gattttttgc tgcaatccca ttgccagttc atcgttataa ggcgttttca aattctgata
     1561 acgcgtcagg gttttattac ctgataccga ttccgtccag ctattgcgga tatcacgtaa
     1621 tcccatatca agaatattcc cgccatagta acggttataa ccggcggtaa tcattgaggt
     1681 ttgattagca aaaatatccc attccgtcat aaagcgcggg gagatattgt ggtttgacag
     1741 atagttgtca tagtcgtaac gcacaccggg cattaacgac acattccgcc agctaatgtg
     1801 atccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt tatggtaaat
     1861 ggtatggtta gttttctttc ctgcagcatt aatcacatag gattcagact ggttatggcg
     1921 ttcagtccac gcatcggaat agatgtattc cgcgccgaag tagggttgat gcgaaacatt
     1981 acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat ctacagcctg
     2041 ggaaatgtgt cctaatccgc cacgggtgca acgccctgta atatcaccat atgtacatga
     2101 aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat gatcccaacc
     2161 aacggtggta cgcagtttgg cccatgcgag ctgcgtatcc atatcccatg ccagaccgta
     2221 ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat aatcacggct
     2281 ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa accaggtaaa
     2341 tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggcga caatgccgtc
     2401 attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac cggcggtaac
     2461 gccgaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct ttttaaaatc
     2521 tggggagtaa taagtacttc ctgaagagcc ttgattaaat gcgctcttgt tattctcatc
     2581 gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccaatttaa ccttgctatc
     2641 atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc gaccaaattc
     2701 aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat caagataata
     2761 cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat tcgctgggtt
     2821 caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg gcgacgcacc
     2881 gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac tttgcgttga
     2941 atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat tcccggtcgg
     3001 taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta cagggacagg
     3061 agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat cgacggcctg
     3121 cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca tttatttata
     3181 tctccgtaca gcagggcttt tattgttgtt ataaaaccgt gctaatgtca caaatatcat
     3241 cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt gtgggtagtt
     3301 tttcacgcac taaacgcagt aagcgtatag cctcttgttc ctcaagatga gaggtagttt
     3361 cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg atacgttgtt
     3421 tttcgccgct ggaaagaata tctccccagc gatcgtgctc atgaatacgc gcagccaatt
     3481 tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg ggcaggggaa
     3541 gtgctttaca aataatctct ttcagtaagc cgcttttgat taacggtgtt tgtgacacat
     3601 accaactgtc agcaggagaa gaaatatcac ctttaaacca cggccagcag tgggataatg
     3661 ttttaagcag tgtggttttt cccgcaccag agtagccttt cagtaatagc catttgcctg
     3721 gcgaaacatg aaagttcaag ttctctaata tgatcttatt atcaggcgta cgaatactcg
     3781 cattagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg cgctgttcag
     3841 tgagttgatg gaactcatac aagcgatcga taaccgcagc cagttcagca agttcgtcat
     3901 atttataaat aaaccagctt aaattgttcg atactagcat aaatgcctgg cgcgatttca
     3961 tcagtccgcc cagattgatc tgcccgctaa taaactgcgg caataacaga aagtagggaa
     4021 gaacgctaag cgagcgcgaa taaatatttt gccagtaatc aagccaccgc tgacgattca
     4081 ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gcgcaactcc tggcgttgta
     4141 gagactcagc attgcttagc gcaatcagtt ctgcctgctt attatgctgc acaagattag
     4201 ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata cgtttaccca
     4261 ccttatgggt aaataaagtt ccaccgatca caatgagcac gacggtatag accatatacc
     4321 cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg ctttgccaaa
     4381 gaataacggt aaaggtgatc agcatgctaa gtgactggat gaagccaaat gaaagactga
     4441 gcgttttgct gatcagtaag agaatgtctt cagcgatacg ttggtcaggg ttatctgtat
     4501 ttttatgctc gccgtagatc tgcgtgaagt agtagttttt atctgcaaac cagcgattaa
     4561 ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag tagtttaatt aaccatgttt
     4621 tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaagg acaagctgcc
     4681 aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc cagtcgttta
     4741 accagacctg aattttaacc acgccaagga tcattgcgag gataataatg attaacaaca
     4801 ctgaagtttt attattctta cgcagccaaa agggcttcag cagacagaga tactttgcta
     4861 tcaacataca aaacgtaatg ggaatggtta tcattag
//
LOCUS       DAPPNJ010000257         4832 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000257 DAPPNJ010000000
VERSION     DAPPNJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.259"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1174
                     /gene="proV"
                     /locus_tag="RPM73_004123"
     CDS             <1..1174
                     /gene="proV"
                     /locus_tag="RPM73_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDX6522406.1"
                     /translation="YKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEG
                     EIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVF
                     QSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMR
                     QRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEA
                     MRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNG
                     LIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALI
                     DAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGGNNG"
     gene            1167..2231
                     /gene="proW"
                     /locus_tag="RPM73_004124"
     CDS             1167..2231
                     /gene="proW"
                     /locus_tag="RPM73_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDX6522407.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2289..3281
                     /gene="proX"
                     /locus_tag="RPM73_004125"
     CDS             2289..3281
                     /gene="proX"
                     /locus_tag="RPM73_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDX6522408.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3573..4757
                     /locus_tag="RPM73_004126"
     CDS             3573..4757
                     /locus_tag="RPM73_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6522409.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
BASE COUNT         1066 a         1277 c         1295 g         1194 t
ORIGIN      
        1 ttataaaata tttggcgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc
       61 gaaagaacaa attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc
      121 cattgaagaa ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat
      181 ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt
      241 ggatattgcc aaaatatccg atgccgaact ccgtgaggtg cgcagaaaaa agattgcgat
      301 ggtcttccag tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg
      361 tatggaattg gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg
      421 tcaggtcggg ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg
      481 tcaacgtgtg ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga
      541 agccttctcg gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt
      601 acaggcgaaa catcagcgca ccattgtctt tatttcccac gatctcgatg aggctatgcg
      661 tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga
      721 tgaaattctc aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat
      781 tagtcaggta ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa
      841 aacccctggc ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata
      901 tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca gtttccatcg attcgcttaa
      961 aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt
     1021 cgatgcacaa acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt
     1081 gcccgtggtc gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg
     1141 tgctttagat cgtgaggggg gaaataatgg ctgatcaaaa taatccgtgg gataccacgc
     1201 cagcggcgga cagtgccgtg caatccgcag acgcctgggg tacaccggcg actgcaccga
     1261 ctgacggcgg tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta
     1321 atattctcga tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga
     1381 tcgactgggt tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt
     1441 acattctcaa cggtttccag caattgctgc tgggtatgcc cgcaccggtg gcgattatcg
     1501 ttttcgctct catcgcctgg cagatttccg gggtcggaat gggcgtggcg acgctggttt
     1561 cgctgattgc catcggcgca atcggtgcct ggtcgcaggc catggttacc ctggcgctgg
     1621 tgttaaccgc cctgctgttc tgtatcgtca ttggtttgcc gctggggatc tggctggcga
     1681 gaagtccgcg agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag
     1741 cgtttgttta tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg
     1801 tggtgacgat catctttgct ctgccgccga ttatccgtct gaccattctg gggattaacc
     1861 aggttccggc ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc
     1921 tgttcaaagt tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc
     1981 tgatgctggc cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc
     2041 agatggtact tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg
     2101 ggattgtgat cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac
     2161 gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat
     2221 tcattaagta actctgcact tgcccggtga cgccgggcat tatcaccctg ccaaaaaaag
     2281 gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac
     2341 aaacttttgc tgccgacctg ccgggcaaag gcattacagt taatccagtt caaagcacca
     2401 tcaccgaaga aaccttccag acactgctgg tcagtcgtgc gctggagaaa ttaggttata
     2461 ccgtcaataa acccagcgaa gtagattaca acgttggcta cacctcgctt gcttccggcg
     2521 atgcaacctt caccgccgtg aactggacgc cgctgcatga caacatgtac gaagctgccg
     2581 gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcggca cagggttacc
     2641 tgatcgataa gaaaaccgcc gaccagtata aaatcaccaa catcgcacaa ctgaaagatc
     2701 cgaagatcgc caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta
     2761 accctggctg gggctgcgaa ggtgccatca accaccagct tgccgcgtat gaactgacca
     2821 acaccgtgac gcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct
     2881 acaaagaggg caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc
     2941 tgaagccagg gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata
     3001 aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc
     3061 atatcgttgc caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca
     3121 ttatgcagtt gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag
     3181 cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt
     3241 tcgatggctg ggtgaatgag gcgctggcag cgcagaagta atttttattc aggcggataa
     3301 ggcgtttttg cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtctt
     3361 atcatgccta caaaatacac tcattccgta tggcggataa ggcgttttcg ccgcatccgc
     3421 cgttctgtgc acaatgcctg atgcgacgct ggcgcgtctt atcatgccta caaaccacat
     3481 cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta ttattgattt
     3541 ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat gagcttagcc
     3601 cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtcgccagt aactattacg
     3661 ctcagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt tcggcaggct
     3721 ttattgttac cgccgcgcaa ttgggctatg cagcaggtct gctgtttctt gttcccctcg
     3781 gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggcg gcaggcggca
     3841 tgttaatcac tgccagcagt cagtcgctgg cgatgatgat cctcggtacg gcattaaccg
     3901 gtttattctc tgtcgtggca caaattctgg ttccgctggc agcgacgctg gcttcaccgg
     3961 acaaacgcgg caaagtggtt ggcactatta tgagcggtct gctgttgggg atcttgctgg
     4021 cacggacagt tgccggattg ctggcgaatc tcggcggctg gcgcaccgtc ttttgggttg
     4081 cctcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca caaatgaaat
     4141 cagaaaccca cctcaactac ccacagttgt taggttccgt tttcagcatg tttatcagcg
     4201 ataagatcct gcgcacccgc gcattgctgg gctgcctgac ctttgccaac ttcagcattc
     4261 tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc gatggtgtca
     4321 ttggtctgtt tggacttgcg ggagctgccg gagcgttggg cgctcgtccg gcgggcggtt
     4381 ttgccgataa gggtaaatcg caccacacca caactttcgg tctgctgctg ctattacttt
     4441 catggctggc gatctggttt ggtcacactt ccgtactggc gctgattatc ggcatcctgg
     4501 tgctggatct caccgtacag ggcgtgcata tcactaacca gacggtaatt tatcgaatac
     4561 accctgatgc gcgtaatcgc ctgaccgcag gttacatgac cagctacttt attggcggtg
     4621 ccgccggttc gctaatttca gcctcagcct ggcaacatgg cggttgggct ggcgtttgtc
     4681 tggctggcgc gacgattgcc ctggttaact tactggtctg gtggcgaggt tttcatcgtc
     4741 aggaagccgc aaattaagca atcagcaacc gttttcgtgg ctttacactt ataagggtgt
     4801 taagaagccc atcagtctga taaggttaag at
//
LOCUS       DAPPNJ010000258         4795 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000258 DAPPNJ010000000
VERSION     DAPPNJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.260"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(184..560)
                     /gene="rnpB"
                     /locus_tag="RPM73_004127"
     ncRNA           complement(184..560)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RPM73_004127"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(593..1738)
                     /gene="garK"
                     /locus_tag="RPM73_004128"
     CDS             complement(593..1738)
                     /gene="garK"
                     /locus_tag="RPM73_004128"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDX6522410.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1835..2725)
                     /gene="garR"
                     /locus_tag="RPM73_004129"
     CDS             complement(1835..2725)
                     /gene="garR"
                     /locus_tag="RPM73_004129"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDX6522411.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIVGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2755..3525)
                     /gene="garL"
                     /locus_tag="RPM73_004130"
     CDS             complement(2755..3525)
                     /gene="garL"
                     /locus_tag="RPM73_004130"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDX6522412.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATDGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3541..>4795)
                     /gene="garP"
                     /locus_tag="RPM73_004131"
     CDS             complement(3541..>4795)
                     /gene="garP"
                     /locus_tag="RPM73_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDX6522413.1"
                     /translation="AVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQI
                     PGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPS
                     FPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGGI
                     GFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQ
                     LLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGG
                     VLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGK
                     GFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFV
                     GCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1158 a         1389 c         1148 g         1100 t
ORIGIN      
        1 aagcatataa ctcagagaat caatatttta ccagagagga atatacgtat gattacgacc
       61 ttttaaatgc aatatagact taataacttc ttacaaagtc tattattaat accgtagata
      121 tttattcatc ttaccctgtg caccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa
      181 atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
      241 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      301 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      361 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcgcgggc catcggcggt
      421 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      481 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      541 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      601 cgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      661 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      721 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      781 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      841 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      901 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      961 acgccatcag cgccgcgccc ataccgcccg cagctcctgc gccggggaca tctttcacat
     1021 caacatgcag cgctttttta atgacatcag catagtgaga gaggttattg tccagctcaa
     1081 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
     1141 ccaccagcgg attggtaaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1201 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1261 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1321 catttgtagc gctgccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1381 gcaggattaa ctcacctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1441 gtaccagttc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1501 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1561 cggaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaacag
     1621 gaacagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1681 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1741 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1801 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1861 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgcacgca
     1921 gtgcctgcat catctccata accgcagctg tgagcggcag ttgtgcgccg acgccgtgag
     1981 aagtatccag cgcattcgcc agatccttaa tatgcagatc aatgcggaag cccggcttga
     2041 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagcccac
     2101 cgcgaattgc ctgataaacc aggtccgggt taacgcccgc tttggttgcc agcgttaacg
     2161 cttctgacat cgcggcaata ttcagcgcca caatgacctg atttgccagt ttggtgacgt
     2221 tacctgcacc gatttccccg gtatgcacca cggaacccgc catcgctttc atcaaatcat
     2281 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgacgatgg
     2341 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2401 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2461 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2521 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2581 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2641 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2701 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2761 tttaaaggta tcagccagtt tctgagtggc agagcggaag acgccgagat cgctgccgac
     2821 agccacaaac gtcgcacccc attccagata acgacgcgca tcggcttcga ccggtgcgag
     2881 gataccactg ggtttgccgt gcgcactggc acggttaaaa atgtgctgaa ttgctttttg
     2941 tacatccggg tgcgaggcat tgccgagatg gcctaatgcc gcagccagat cgctggggcc
     3001 gacgaagata ccgtctacac catcggtagc ggcaatggca tcgacgttat ctacgccctg
     3061 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     3121 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggcgg
     3181 gtaacgagtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3241 gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt tggtcggcac
     3301 tcgcactact ggcgcgctgg cgctgccttt cagggccatt aactgcggaa taaacgtgga
     3361 aatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3421 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3481 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3541 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3601 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3661 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3721 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3781 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3841 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3901 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3961 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     4021 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     4081 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     4141 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4201 catatcgacc accgcgccat tttcagagat aaacttcagc tcttccgcag acatacgtgg
     4261 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aaccaatccc
     4321 ccccataacg gtgaagacgt gctcccagcc ccaggcgaaa gtcagccagc caagcagcgg
     4381 cgaaaagagc gccagcgaga aatattgcgc cgagttaaag atggcggagg cagtaccacg
     4441 ttcttttgtc ggaaaccagg cggcgacaat acgggcgttc gccgggaatg atggcgcttc
     4501 cgagaacccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4561 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4621 aacttttttc gagccaaatt tatcaagcag ccagccgccg ggaatttgca tcagcaggta
     4681 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4741 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcg
//
LOCUS       DAPPNJ010000259         4777 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000259 DAPPNJ010000000
VERSION     DAPPNJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.261"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            258..758
                     /gene="tssB"
                     /locus_tag="RPM73_004132"
     CDS             258..758
                     /gene="tssB"
                     /locus_tag="RPM73_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDX6522414.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            793..1017
                     /locus_tag="RPM73_004133"
     CDS             793..1017
                     /locus_tag="RPM73_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522415.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1068..2273
                     /gene="tssC"
                     /locus_tag="RPM73_004134"
                     /pseudo
     CDS             1068..2273
                     /gene="tssC"
                     /locus_tag="RPM73_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2360..2785
                     /locus_tag="RPM73_004135"
     CDS             2360..2785
                     /locus_tag="RPM73_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX6522416.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            2782..3132
                     /gene="tnpB"
                     /locus_tag="RPM73_004136"
     CDS             2782..3132
                     /gene="tnpB"
                     /locus_tag="RPM73_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX6522417.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            3163..4776
                     /locus_tag="RPM73_004137"
     CDS             3163..4776
                     /locus_tag="RPM73_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDX6522418.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT         1231 a         1189 c         1259 g         1098 t
ORIGIN      
        1 tctatattta aattattgcc ataatacacc tgacgcgaat tgaacaggtt catacaatct
       61 atttaacgct tgcagaatac ccctctttac gatcgataaa aagtcctttt cttatttatt
      121 ttgaagcgta aaatgcgggg tgatgaagga gtggtgattt tattttcacc gccatcaagg
      181 cggcatttcc gatatttttt gcggtacgaa tatgccgtgt tcatggcctg tacgagataa
      241 catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgcct cgcgagcgta
      301 ttaatatatc gtatgtccca aaaacagatg gccagacggc tgaagtggaa cttccgctta
      361 acatgctcgt tgtcggcgat accggaaata cgcaggaaac atcctcactg gatgaacgtc
      421 aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca gccattggcc
      481 tcaattttac cgtccccgcc acgctgaagg gaagcacaac ggacgatgag ctgaatgtgg
      541 cgctgaatat caagtcactg gatgattttt caccagatag cgtggcccgg caggttccgg
      601 aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg ccgatgggta
      661 accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag tcccgcgagc
      721 agttgctcaa agaaatcggt ctggtgagca ataaataacc accactattt cattaaacag
      781 ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct ctctgttgca
      841 gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat caaccaggat
      901 ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt ggatgagatt
      961 atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact tcactgacgg
     1021 cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg tctttacagg
     1081 aagaggaact cgtatccagt catgccgggc agcctgagca ggcatcgtcg ctgcttgatc
     1141 aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt gcccgccagg
     1201 gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa ccggtcaata
     1261 aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc cagatggatg
     1321 tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca ctgaaaacca
     1381 tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat gtcaccaagc
     1441 aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga ttttataagc
     1501 acgtttattc gtccggtttc ggacagtttg gcggtgaacc catcgctgcc gtgctgggcg
     1561 cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc agtgccgtgg
     1621 gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg gggctgaact
     1681 cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt ccggcctaca
     1741 ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt acggcacccc
     1801 gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac tttaactatt
     1861 acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc tggatgctgg
     1921 catgcaatat cgcagacagt tttgccaaat accgctggtg tccaaacatt atcggtccgc
     1981 aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt cgaaacgatg gggcagattc
     2041 aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa ctggctgaag
     2101 agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt ttctctgcaa
     2161 actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac tataagctgg
     2221 gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc aagggtaagc
     2281 gtaaactgac cgccgtatgt agccatcaga cgagaattgg taacttagac gcccatctga
     2341 tatagacgga catctaagta tggaattaca ggactggcga aaagaacctc gtaaaaacta
     2401 ttcgaatgaa ttcaaacttc gtatggtgga actggcatca caacctggag cttgtgttgc
     2461 acagattgca cgtgaaaatg gcgtcaatga taatgttatt ttcaaatggc tcaggctctg
     2521 gcagaacgaa gggcgtgttt cgcggcgtct tccggtaacg acctcttctg acactggcgt
     2581 tgaattatta cctgtagaaa taacgccgga tgagcagaaa gaacctgtgg cggccattgc
     2641 gccgtcttta tccacttcca ctcagaccag agtcagtgcc agttcctgca aggtggaatt
     2701 ccgtcacggt aacatgacgc tggaaaatcc atcgccagag ctgctcacag tgttgatccg
     2761 tgaactgacc gggaggggaa gatgatctca ctcccatcag gtacccgtat ctggctcgtt
     2821 gccggcgtta ccgatatgcg taaatccttc aacggactgg gagaacaggt acaacatgtg
     2881 ctgaatgata atcccttctc cggtcacctg tttatcttcc gtggccgacg gggtgacacc
     2941 gtcaaaattc tttgggctga tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa
     3001 ggccagttta tctggcctgc ggtacgtgac ggcaaggtat ccattacccg ctcgcaactg
     3061 gcaatgctcc tcgataagct ggactggcgt cagccaaaaa catccagccg taactcactg
     3121 acaatgttgt aaaaaactcc tgaccgcatt ataaaaacgg tcatgagtca gaaatacctc
     3181 attcgcatcg cagagctgga aaggttgctc tctgagcagg ctgaagccct ccgtcagaaa
     3241 gaccagcaac tgagtctggt tgaagagacg gaagccttcc tgcgctctgc actgacacgt
     3301 gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc tcagatagaa
     3361 aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga agttgaactg
     3421 gctgaggctc tgctgaaaca acgtgaacag gacagcgatc gttacagtgg gcgggaagac
     3481 gatcctcagg ttccccgcca gttgcgacag tcgcgccatc gtcgtccgtt accggcacac
     3541 cttccccgtg aaatacaccg cctggagcca gaagaaagct gttgcccgga gtgtggcggt
     3601 gagctggatt atctggggga agtcagcgct gaacagctgg aactggtgag cagtgccctg
     3661 aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg tattgttgaa
     3721 gcaccggcgc cgtcccgccc gatagagcgt ggtatcgcgg gccccggatt acttgcccgc
     3781 gtgttaacgg gaaaatactg cgaacatctg ccactgtatc gtcagagtga aatctttgcc
     3841 cgccagggtg tcgaactgag ccgggcctta ctctccaact gggttgacgc gtgctgccag
     3901 ttaatgacac cggtgaatga tgccctgtac cgttatgtaa tgaacacccg caaggttcac
     3961 actgatgaca caccggtaaa ggtactggca ccgggtcaga aaaaggcgaa aacagggcgt
     4021 atctggacgt atgtccggga tgatcgcaat gtgggttcgt catctcctcc agcggtctgg
     4081 ttcgcgtact cgccgaaccg gcaggggaaa cacccggagc aacacctccg ccccttccgg
     4141 ggtatcctgc aggcggatgc gttcacaggt tacgacaggt tgttcagtgc agaacgtgaa
     4201 ggtggtgcac tgacagaagt tgcgtgctgg gcccatgccc ggcgaaaaat ccacgatgta
     4261 tacatcagca gcaaaagtgc gacggcagaa gaagccctga agcgaatcag tgaactgtac
     4321 gccatcgagg atgaaatacg gggattaccg gagtcagagc gtcttgccgt caggcagcag
     4381 cgaagcaaag tgttactgac gtcgctgcat gaatggatgg tggagaagaa tggtacgctg
     4441 tcgaaaaaat ccagactggg cgaagcgttc agctatgtac tgaatcagtg ggatgccctc
     4501 tgttattaca gtgatgacgg tctggcggag gcggataata atgctgcgga aagagcgctt
     4561 cgtgcagtct gtctcggaaa gaaaaacttt atgttctttg gcagcgatca cggcggcgag
     4621 cgtggagcac tgttgtacgg gctgatcggc acctgccgtc tgaacggtat cgatccggaa
     4681 gcgtatctgc gccatatcct gagcgtactg ccggaatggc cttccaaccg agttgacgaa
     4741 ctcctgccat ggaacgtagt actcaccaat aaataag
//
LOCUS       DAPPNJ010000260         4741 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000260 DAPPNJ010000000
VERSION     DAPPNJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.262"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..618
                     /gene="hprR"
                     /locus_tag="RPM73_004138"
     CDS             <1..618
                     /gene="hprR"
                     /locus_tag="RPM73_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDX6522419.1"
                     /translation="GLSEAGYVIDAVSDGRDGLYLALKDDYALIILDIMLPGMDGWQI
                     LQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQLRQHHA
                     LNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEIIPRTVIASEIWG
                     INFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            618..1976
                     /gene="hprS"
                     /locus_tag="RPM73_004139"
     CDS             618..1976
                     /gene="hprS"
                     /locus_tag="RPM73_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDX6522420.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(2084..2935)
                     /gene="hchA"
                     /locus_tag="RPM73_004140"
     CDS             complement(2084..2935)
                     /gene="hchA"
                     /locus_tag="RPM73_004140"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDX6522421.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3527..4741)
                     /gene="ompC"
                     /locus_tag="RPM73_004141"
     CDS             complement(3527..4741)
                     /gene="ompC"
                     /locus_tag="RPM73_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDX6522422.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDKSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQSNNESGSDGLFGQEGSGSGDGRSLAKENGDGFGMSTSYDFDFGLS
                     LGAAYSNSDRSNNQAARGYGDGNHFYGNSYAGGGTAEAWTVGAKYDANNVYLAAMYAE
                     TRNMTAYGSSDSHSADGKLGGGGIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLG
                     GQEMYNNGNYHYTNKDLVKYVEVGATYYFNKNMSTYVDYKINLLDNDDDFYANNGIAT
                     DDIVAVGLVYQF"
BASE COUNT         1330 a          990 c         1094 g         1327 t
ORIGIN      
        1 gggctttccg aagcgggtta tgtcattgat gccgtttctg atggcagaga tgggctttat
       61 cttgcgctga aggatgatta tgcattgatc attctggata ttatgcttcc gggtatggat
      121 ggctggcaga tcttacaaac gttaagaaca gcaaagcaaa cccctgttat ttgccttact
      181 gcaagggatt ctgtcgatga cagagtcaga gggctggaca gtggagcaaa tgattatctg
      241 gtaaaacctt tttcattttc tgagttgctg gcaagggttc gggcacaatt aaggcaacat
      301 cacgctttga attcaacatt agaaatcagt ggcttaagaa tggactctgt aagtcaaagt
      361 gtgagcaggg acaatatcag tattacactg acgcgcaagg agtttcagtt actttggcta
      421 ctggcctcca gagctggcga aattataccc agaacggtta ttgcgagtga aatttgggga
      481 atcaactttg atagtgatac caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa
      541 gttgatgatc cttttcctga aaagctaatc gccacaatcc gggggatggg ctattcattc
      601 gtagcggtaa aaaaataatg aaaagattat ctataaccgt ccgtttaacc ttgcttttta
      661 tattgctgct gtctgttgct ggcgctggaa ttgtctggac tctctataat ggcctggcaa
      721 gtgagttgaa atggcgcgat gatacaacac tcattaaccg gacagcacag atcaagcagt
      781 tgttaattga tggggtaaat ccagatacgt tacctgtata ctttaaccgg atgatggatg
      841 ttagtcagga tatcttgatc attcatggtg atagcatcaa taaaattgtt aaccggacaa
      901 atgtcagtga tgacatgtta aataacatac ctgctagtga gacaatcagc gcagctggca
      961 tttacagaag cattattaat gatactgaga ttgatgcttt acgaattaat attgatgaag
     1021 tttcgccatc attaacggtt actgtggcta aattggcttc agccagacat aacatgcttg
     1081 aacagtataa aattaatagc attataattt gcattgtcgc cattgtactt tgctcagtat
     1141 taagtccgct gttaatcaga acgggattac gagagatcaa aaagttgagt ggtgtaacgg
     1201 aagcgctgaa ttataacgat agccgggagc ctgttgaggt tagcgcatta ccgagagaac
     1261 taaaacctct tgggcaggcg ttgaataaaa tgcatcaagc cttagtcaaa gattttgaac
     1321 gcctaagtca atttgctgac gatctcgctc atgaacttag aacgcccatt aatgcattac
     1381 tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa
     1441 ttgccggtaa cattgaagag ctggaaaata tttcgcggtt aacagagaac atactgtttc
     1501 ttgcccgggc agataaaaac aatgttttgg tgaaactgga ctcgctttct ctcaataagg
     1561 aagtcgaaaa tttgttggat tatcttgaat acctttcaga cgagaaagag atttgcttta
     1621 aggtcgagtg caatcagcaa atctttgcgg ataaaatttt actgcaacga atgttatcga
     1681 atcttattgt taatgccatt agatattcac cagaaaaatc gcatattcat ataaccagtt
     1741 ttcttgatac caacggctat cttaatattg atgtcgccag tcctggaacg aaaattcatg
     1801 agcctgaaaa actcttccgt agattttggc ggggagataa ttcgcgtcat tccgtaggtc
     1861 agggactagg cctttcttta gtcaaagcga ttgccgaatt acatggggga agtgctacgt
     1921 atcactatct caataagcat aatgtgttcc ggattatgtt accgcaaaga aattaacata
     1981 gggcttcagt acgccataaa tcaaccatga aaaaacgcag ccacgtcgtg agtattaacg
     2041 tggctgcgat tgattatgcg cttacattca tacgtaacag ggattaaccc gcataagctg
     2101 ccagcatttc ctgcgccgcc agtttaccca acgcattcgc tgcgaaagga ctgtcgccgg
     2161 tgagaacttt acggtcctta tgtactcgcc cggcgatgtc gtcattaata atgttcatgc
     2221 ccattttctt cagctcttcg ccgaagtacc aggtgagatg acccggcata tagcctatct
     2281 ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat ggaataacca ttcagtgggt
     2341 tatcgccgtg gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag ataacaaaac
     2401 ggtcattttt gattgcccac tgtaaagcag cagccacgtc ctggctttct ggtagaccaa
     2461 taagtgcgcc atgaccacca ggaacaaaga ttgctgcata ttcgctatca gcgttgaggc
     2521 tggcgacaac atccgcgagt ttcttcggat tgcggaacaa cgatttatgc tgctcaaaga
     2581 atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg gtcatcagac
     2641 cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac aacggcagca
     2701 acgtttcaat cggatggtta ccggtcgaga acagttttcc gttatcggtc ggcaaataac
     2761 gttcgtccgc ggcgatcacc aaaattttat gtttaccgcg atacggtttt ggatagtcca
     2821 cgccatcaag atcagagaca ggactggtat attggctaag cgaatattct gaagggaaga
     2881 atgcattatc ttcagcaata tcgacctgcg gatttttact tgtttgaaca gtcatagtgt
     2941 attccttatt gttgcttagt tagggtagtc actatatttg cgactgagct ggtcgctatt
     3001 tttaaccagg ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg ggtgtaagta
     3061 tgtaatgatg atgctgcact ggtgttgaat attattcacg ccaaattttc cttattgagc
     3121 aacgtcgtcg caagtggcct caatgatttt agaccattat cagaggtacg gggcggcggg
     3181 gagagattta gtgatgtgga atattgtggt gctgatgaat atttatatcc cggattggta
     3241 gagaaaaagc ttatttcatg cactattttt gcaaatattt ctatatggga ggcggttttg
     3301 ttgcagatta attcataaag aaatgcattt gggtcttggc tggaagagtc gctggtagat
     3361 gttattgtgc cagcaggggc agggtaaggt gctgtatcga tatgcattgg caattgcgtg
     3421 tgccgcagat ggagatgaaa tgccaataaa aaagcctgaa ttcgatgagg gacgaactca
     3481 ggctagtgtt tagttttaca gtaacacgcc gcagcacgtt ggctgattag aactggtaga
     3541 ctaagcccac agcaacgata tcatcggtag cgatgccgtt gtttgcgtag aagtcatcgt
     3601 cgttgtccag caggttgatt ttgtaatcaa cataggtgga catgtttttg ttaaagtagt
     3661 aggttgcacc aacctcaacg tatttaacca gatctttatt ggtataatga taattaccat
     3721 tgttgtacat ttcttgacca cctaaatcct tacctttaga ttggaggtaa gcaattgaag
     3781 gacgcaggcc gaaatcaaat tggtactgag caacaacttc aaagttctga gttttgttag
     3841 cgataccacc gccgccaagt ttcccatcgg ctgaatgaga gtcgctgcta ccataagcgg
     3901 tcatattgcg agtctctgca tacattgctg ctaaatatac attgttggca tcgtatttag
     3961 caccaacagt ccacgcttct gcagtaccac cacctgcata gctattacca tagaaatgat
     4021 tgccatctcc atacccacga gcagcttggt tattggaacg gtcagaatta gagtaagcag
     4081 cacccagact taaaccgaag tcgaagtcgt aggaagtaga cataccgaaa ccatcgccat
     4141 tttctttagc aagactacgg ccatctccgc tgccagatcc ttcttggcca aagagaccgt
     4201 cactgccact ttcattatta ctctgatatt gcagcgcaaa gttcaagcca tcaacctgac
     4261 caaagaagcc attgttacgg taggttgcaa caccgttggt acgaccgttc atgaaattgt
     4321 cggcaccagc ataggtatcg ccaccaaatt ctggcagcat atcggtccat gcttcgatgt
     4381 catatacgac accgtagtta cgaccataat cgaaagtacc attctgacca aaacctaaac
     4441 ctgcgaaagc cagacgggta gcagagttat ctccatcgcc ttctgtgcca tttgcttgca
     4501 ggttgtattc ccactgacca taaccagtga attggtcagc gatctgagtt tcacctttga
     4561 aaccgatacg ggcatatgac ttgtcgccat cgctgccagc atcatcagag aagtagtgca
     4621 ggcccgctac ttttccgtac aaatccagtt tattaccatc tttattatag acttcagccg
     4681 catttgctgc gccagcaact aataacgccg ggaccagcat tgccagaact tttcttttca
     4741 t
//
LOCUS       DAPPNJ010000261         4731 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000261 DAPPNJ010000000
VERSION     DAPPNJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.263"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..366)
                     /locus_tag="RPM73_004142"
     CDS             complement(<1..366)
                     /locus_tag="RPM73_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDX6522423.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILL"
     gene            complement(481..556)
                     /locus_tag="RPM73_004143"
     tRNA            complement(481..556)
                     /locus_tag="RPM73_004143"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Thr,seq:ggt)
     gene            complement(563..637)
                     /locus_tag="RPM73_004144"
     tRNA            complement(563..637)
                     /locus_tag="RPM73_004144"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(602..604),aa:Gly,seq:tcc)
     gene            complement(754..838)
                     /locus_tag="RPM73_004145"
     tRNA            complement(754..838)
                     /locus_tag="RPM73_004145"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Tyr,seq:gta)
     gene            complement(847..922)
                     /locus_tag="RPM73_004146"
     tRNA            complement(847..922)
                     /locus_tag="RPM73_004146"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(887..889),aa:Thr,seq:tgt)
     gene            1284..2234
                     /gene="coaA"
                     /locus_tag="RPM73_004147"
                     /gene_synonym="panK"
     CDS             1284..2234
                     /gene="coaA"
                     /locus_tag="RPM73_004147"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDX6522424.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQILKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2263..3228)
                     /gene="birA"
                     /locus_tag="RPM73_004148"
     CDS             complement(2263..3228)
                     /gene="birA"
                     /locus_tag="RPM73_004148"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDX6522425.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3225..4253)
                     /gene="murB"
                     /locus_tag="RPM73_004149"
     CDS             complement(3225..4253)
                     /gene="murB"
                     /locus_tag="RPM73_004149"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDX6522426.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4556..4671)
                     /gene="rrf"
                     /locus_tag="RPM73_004150"
     rRNA            complement(4556..4671)
                     /gene="rrf"
                     /locus_tag="RPM73_004150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1183 a         1200 c         1085 g         1263 t
ORIGIN      
        1 cagcaggatg tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat
       61 cgcgccgtcc atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg
      121 gcagtctacg tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat
      181 ggtgatacca cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc
      241 accgccgtag gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg
      301 gtcaacgtgg ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt
      361 agacatcgat tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc
      421 aacatgcccg acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag
      481 tggtgctgat aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca
      541 actgagctat atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct
      601 tggaaggctg aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat
      661 tttcattctg cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta
      721 tcgtctcggg ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt
      781 cgaagtctgt gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg
      841 acttgatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct
      901 ctacctactg agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca
      961 tgcaactaaa aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga
     1021 aacagccgaa cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg
     1081 ctcctgctaa accataattc tttttatcag atggaatatc tgccacattg cttttcaacg
     1141 atagcttcct ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc
     1201 ccattaaccc attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag
     1261 ctatgaccgc cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc
     1321 tacagtttga ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg
     1381 aagatgagat cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg
     1441 agatctattt acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc
     1501 aggcagttct ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta
     1561 ttgctggcag tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag gcgctattaa
     1621 gccgttggcc ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta
     1681 atcagattct gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata
     1741 tgcatcgcct ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc
     1801 ctgtttactc gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc
     1861 ctgatatttt aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg
     1921 atccacatca tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg
     1981 aagacttact tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta
     2041 ccgacccgga ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga
     2101 ctgccatgac attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta
     2161 ctcgtgagcg cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca
     2221 gactacgcaa ataattttca ggggagctaa tgctcccctt tcttattttt ctgcactacg
     2281 cagggatatt tcaccgccca tccagggttt tattattcca tcctgctcaa gcaataaagc
     2341 gccctgtttg tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt
     2401 cactgggcga ttaataaaat tatccagctt ttcccagcgc gaaagataag gtgccaatcc
     2461 ttcttgttcg aagagttcca gcgccgcgcg taattcacgt attagcatgg ccgccaacgt
     2521 attacgatcg agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact
     2581 ctcttcaaca cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc
     2641 gccagttttg ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag
     2701 gtcattaggc catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat
     2761 cacgataccg ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca
     2821 gaacatcgac aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc
     2881 acggccagcc tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg
     2941 atcaagaagg tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc
     3001 cagctgaccc aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc
     3061 tttacccgga acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt
     3121 aatagccgcc cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc
     3181 gttcgctaac agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc
     3241 actgcgctca cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta
     3301 aatttttcac ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt
     3361 gcattgtctt cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt
     3421 tgcatccctt ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca
     3481 cccgcctggg ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg
     3541 gcagatacaa cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg
     3601 agtttggtgg tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta
     3661 ggatccagac gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc
     3721 agacctacgg ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga
     3781 tagccaaaac ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca
     3841 caatcaacat aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata
     3901 ggtgatgagc cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc
     3961 tgcaaagtgt attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac
     4021 gcatcaggtt catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga
     4081 tagtcttcca gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct
     4141 gcggttgcat actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc
     4201 tgagcattat gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct
     4261 ttcctgatgc aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg
     4321 acgacaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     4381 atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     4441 gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4501 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     4561 gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac
     4621 ttctgagttc ggcatggggt caggtgggac caccgcgcta cggccgccag gcaaattctg
     4681 ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa t
//
LOCUS       DAPPNJ010000262         4730 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000262 DAPPNJ010000000
VERSION     DAPPNJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.264"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1593
                     /gene="tssI"
                     /locus_tag="RPM73_004151"
     CDS             <1..1593
                     /gene="tssI"
                     /locus_tag="RPM73_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX6522427.1"
                     /translation="AQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRY
                     SAQIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFAR
                     WQMDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVP
                     GRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRV
                     KFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGR
                     TYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVL
                     NNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLV
                     RALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKK
                     DDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNC
                     AASKSPPKTPDEKQDTPDMREY"
     gene            1661..4730
                     /gene="rhsD"
                     /locus_tag="RPM73_004152"
                     /pseudo
     CDS             1661..4730
                     /gene="rhsD"
                     /locus_tag="RPM73_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
BASE COUNT         1079 a         1329 c         1542 g          780 t
ORIGIN      
        1 gcgcaaaaaa gtaccgacca gagcctggtc ctgtgcgaca ccgtgcgtta tctgccggag
       61 tcctttgaga tcccctggaa tccgaacacc cgtaccgagg tgagcaccct ctgcatcagc
      121 cagttccgct acagcgcaca aatccgccct tcttccgtgg tgaccaaaga ctacaccttt
      181 aaacgccccg gctgggccgg acgttttgat caggaaggcc agcaccagga ttaccagcgc
      241 acacagtatg aagtgtatga ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt
      301 gcccgctggc agatggatgg ctggcgaaac aacgcagaag tggcgcgcgg aacaagccgt
      361 tctccggaga tatggccggg acggcgaatt gcgctgacgg ggcatccgca ggcgaacctg
      421 aaccgggaat ggcaggtggt ggcaagtgaa ctgcacggcg agcagccgca ggcggtgccg
      481 ggacgcagtg gttcaggcac cacgctgaat aatcactttg cggtgatccc ggcagacaga
      541 acatggcgac cacagccgtt gctgaaaccg ctggtggacg gcccgcagag cgctgtcgtg
      601 acgggaccgg caggcgagga aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt
      661 aactgggacc gttataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag
      721 gcgtgggcag gcaccggatt cggtaacctg gcgataccgc gtgtgggtca ggaggtgatt
      781 gtggacttcc tcaacggcga tccggaccag ccgatcatta tggggcgcac ctaccaccag
      841 gaaaaccgca cacccggcag cctgccggga acaaagacgc agatgaccat tcgttcgaaa
      901 acctataagg gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag
      961 gtctacatcc acgcgcagaa gaacatgaac accgaggtgc tgaataaccg caccactgat
     1021 gtgataaaca accatgctga aaccattggc aacaatcaga tgattgcggt taccaacaat
     1081 cagatacaga cggtgggtgt taaccagata gagacggtgg gcagtaacca gataattaaa
     1141 gtgggttctg ttcaggtgga aacgattgga cttgttcgtg cgctgaccgt gggcgtggcg
     1201 tatcagacga cggtaggtgg cattatgaac acctcggtgg cactgatgca gtcctcgcag
     1261 atcggtttgc ataaatcgtt gagggtcggg ctgggttatg acgtcaaagt cggaaataac
     1321 gttaccttca ccgttggtaa aacgaaaaag gatgatactg ggcagaccgc gatttactcc
     1381 gccggtgagc atctggagct ctgctgtggt aaggcaaggc tggtgctgac gaaggacgga
     1441 caaatttttc tcaacggcac aaaaattcat ttgcagggta aggagcaggt taatggtgac
     1501 tcactgttga ttaactggaa ctgtgcagcc tcgaaatccc caccgaagac ccctgatgaa
     1561 aagcaggata cgccggatat gagagagtac tgatcccgta cctgttcgga attcagtaac
     1621 aaaccagcat taaaaataaa cagaagaagg aatttgaatc atgagcggaa aaccagcggc
     1681 gcgtcaggga gatatgactc agtatggcgg tcccattgtc cagggttcgg caggtgtaag
     1741 aattggcgcg cccaccggcg tggcgtgctc ggtgtgtccg ggcgggatga cttcgggcaa
     1801 cctggtaaat ccgctgctgg gggcgaaggt gctgcccggc gagacggacc ttgcgctgcc
     1861 cggcccgctg ccgttcattc tctcccgcac ctacagcagc taccggacga agacgcctgc
     1921 accggtgggc gttttcggcc ccggctggaa agcgccttct gatatccgct tacagctacg
     1981 tgatgacgga ctgatactca acgacaacgg cgggcggagc attcactttg agccgctgct
     2041 gccgggggag gcggtgtaca gccgcagtga gtcaatgtgg ctggtgcgcg gtggtaaggc
     2101 agcacagccg gacggccata cgctggcgcg gctgtggggg cgctgccgcc ggatatccgg
     2161 ttaagcccgc atctttacct ggcgaccaac agcgcacagg ggccgtggtg gatactcggc
     2221 tggtcagagc gggtgccggg tgctgaggac gtgctgccag cgccgctgcc gccgtaccgg
     2281 gagctgacgg ggctggcgga ccgcttcggg cggacgctga cgtaccggcg tgaggccgcc
     2341 ggtgacctga ccggggaaat caccggcgtg acggacggtg ccgggcggga gttccgtctg
     2401 gtgctgacca cgcaggcgca gcgtgcggaa gaggcccgca cctcttcgct atcttcttct
     2461 gacagttccc gccctctctc agcctcagcg ttccccgaca cactgcccgg taccgaatac
     2521 ggccccgaca ggggtatccg cctttcggcg gtgtggctga tgcacgaccc ggaatacccg
     2581 gaaaatttac ctgccgcgcc actggtgtgc tacgactgga cgccacgcgg tgagctggcg
     2641 gcggtgtatg accgcagcgg tacgcagatg cgccacttta cttacgatga taaataccgg
     2701 ggccggatgg tggcgcaccg ttatgcaggc aggccggaga tgcgctaccg ctacgacgat
     2761 gcggggcggg tggtggagca acttaacccg gcaggcctga gttaccgcta ccagtatgag
     2821 taggatcgca tcaccgtcac ggacagcctg aaccggcgtg aggtgctgca tacagaaggc
     2881 ggggccgggc tgaagcgggt ggtgaaaaaa gaactggcgg acggcagcgt cacgcacagc
     2941 gggtatgacg cggcaggaag gctcacggcg cagacggacg cggcgggacg gcggacagag
     3001 tacggtctga atgtggtgtc aggcgatatc acggacatca ccacgccgga cgggcgggag
     3061 acgaaatttt actataacga cgggaaccag ctgacggcgg tggtgtcccc ggacgggctg
     3121 gagagccgcc gggaatatga tgaaccgggc aggctggtat cggagacatc gcgcagcggg
     3181 gagacagtac gctaccgcta cgatgacgcg cacagtgagt taccggcgac gacaacggat
     3241 gcgacgggca gcacccggca gatgacgtgg agccgctacg ggcagttgct ggcgttcact
     3301 gactgctcgg gctaccagac ccgctatgaa tacgaccgct tcggccagat gacggcggtt
     3361 caccgcgagg aaggtatcag cctttaccgc cactatgaca accgtggccg gttaacctcg
     3421 gtgaaagacg cacagggccg tgaaacgcag tatgagtaca acgccgcagg cgacctgact
     3481 gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg gggaaaggcg
     3541 gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc cggacgtgtc
     3601 atcagtctga ctaacgagaa cggcagccac agcgacttca gctacgatgc gctggaccgg
     3661 ttggtacagc agggcggctt tgacgggcgg acgcaacgtt atcattatga cctgaccgga
     3721 aaactcacgc agagtgaaga tgaggggctt gtcaccctct ggtactacga tgaatcggac
     3781 cgtatcactc accgcacggt gaacggcgaa ccggcagagc agtggcagta tgatgaccac
     3841 ggctggctga cagacatcag ccacctgagc gaaggccacc gtgtcgctgt ccactacggc
     3901 tacgacgata agggccgcct gaccggcgaa cggcagacgg tggagaaccc ggagacgggg
     3961 gaactgctgt ggcagcatga gacgaaacac gcctacaacg aacaggggct ggcaaaccgc
     4021 gtcacgccgg acagcctgcc gccggtggag tggctgacct acggcagcgg ttatctggcg
     4081 ggcatgaagc ttggcgacac gccgctgctg gagtacacgc gcgaccgcat gcaccgggag
     4141 acggtgcgca gctttggcag catggcaggc agtaatgccg catataaact gaccagcaca
     4201 tacacccccg caggccagtt acagagccag cacctgaaca gcctggtgta tgaccgtgac
     4261 tacgggtgga atgacaacgg cgacctggtg cgcatcagcg gcccgcgaca gacgcgggaa
     4321 tacggctaca gcgccacggg caggctggag agcgtgcgta ccctcgcacc ggacctggac
     4381 atccgcatcc cgtatgccac cgacccggcg ggaaaccgcc tgccggaccc ggagctgcac
     4441 ccggacagta ccctcacggc gtggccggat aaccgcatcg cagaggatgc gcactatgtt
     4501 taccactatg atgaatacgg cagactgacg gagaagacgg accttatccc ggcgggtgtt
     4561 atccggacgg acgacgagcg gacccaccac taccactacg acagccagca ccgcctggtg
     4621 ttccacacgc ggatacagca tggcgagcca ctggtcgaga gccgctacct ctacgacccg
     4681 ctgggacggc gaatggcgaa acgggtatgg cggcgggagc gtgacctgac
//
LOCUS       DAPPNJ010000263         4722 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000263 DAPPNJ010000000
VERSION     DAPPNJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.265"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            10..732
                     /gene="sdiA"
                     /locus_tag="RPM73_004153"
     CDS             10..732
                     /gene="sdiA"
                     /locus_tag="RPM73_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDX6522428.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(799..1023)
                     /locus_tag="RPM73_004154"
     CDS             complement(799..1023)
                     /locus_tag="RPM73_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDX6522429.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1010..1186)
                     /locus_tag="RPM73_004155"
     CDS             complement(1010..1186)
                     /locus_tag="RPM73_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522430.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            1482..2138
                     /gene="uvrY"
                     /locus_tag="RPM73_004156"
     CDS             1482..2138
                     /gene="uvrY"
                     /locus_tag="RPM73_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDX6522431.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            2135..3967
                     /gene="uvrC"
                     /locus_tag="RPM73_004157"
     CDS             2135..3967
                     /gene="uvrC"
                     /locus_tag="RPM73_004157"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDX6522432.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            4024..4572
                     /gene="pgsA"
                     /locus_tag="RPM73_004158"
     CDS             4024..4572
                     /gene="pgsA"
                     /locus_tag="RPM73_004158"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDX6522433.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
BASE COUNT         1197 a         1078 c         1216 g         1231 t
ORIGIN      
        1 cggtttacta tgcaggatac ggattttttc agctggcgtc gcacgatgct gttgcgtttt
       61 cagaggatgg aggccgcaga agaggtctac catgaaatag agcttcaggc tcagcagctg
      121 gagtacgatt actattcgtt atgtgttcgc cacccggtac cattcacgcg acctaaagtg
      181 gctttctaca ccaattaccc tgagtcgtgg gttagttatt atcaggcaaa aaattttctc
      241 gcaattgatc cggtgctgaa ccctgaaaac tttagtcagg gccatttaat gtggaatgat
      301 gacttattca gcgaagcaca gccgttatgg gaagccgcgc gcgcacatgg tttacgccgc
      361 ggtgtcactc agtatttaat gctgccaaat cgggcgttgg gctttttgtc cttttcccgt
      421 tgcagcgctc gcgaaatacc cattcttagt gatgaactgc aattaaaaat gcagttactg
      481 gtgcgcgaaa gtctgatggc tctgatgcgt ttaaatgatg aaatagtgat gacgccagag
      541 atgaatttca gcaagcgcga aaaagaaatt ctgaaatgga cggcggaagg gaagacatca
      601 gcagagatag cgatgatttt gtcaatctct gagaatacgg tcaatttcca tcagaaaaac
      661 atgcagaaaa aaatcaatgc accaaataag acccaggtgg cctgttacgc ggccgctact
      721 ggcttaattt gatcgctttt ctttctgcgt gccagatgca aaaaccggct gaaaggcacg
      781 ctctcagccg gttttatatt actgacggta ggcttgttta atttgcttaa ccgttttgga
      841 aaatactgca gcctgggttt cgtcttcgat cagcgcaagc tgtttttcca tctttaacat
      901 cacgcggccc gcgtcagctt gtcccatcgc ctgcagcatc agcgtcagca tcgctttcag
      961 gcaggagact tcatttgcca gttcttgatt attctcggca gtagaaaaat caggcgtact
     1021 catttatttt cctcgtcatg ttgcaatgaa aatttgcggt gaaaaatgtt aaggcggcgg
     1081 agtataccat aagctttgct aaaaatagca gtggttgttt tttgagcgtg atatcggcag
     1141 tgctataaaa tacgttaata taatgaccaa taaatatttt tatcatgaat gttttttgcc
     1201 cgtattgttc gggttaatta atgttacata ttcagcgggc tgattttcat ttttgctgaa
     1261 taaagtcaat ttttgtcaca tttcatcgta gggcttactg tgaaacgatc cggtaagccg
     1321 ttggtgacgg gcgtgaccat aactgtggac aatcgaattg acaaaaacga gagaaaaatc
     1381 gaatacccac catttttaac gtttcaaagt tgcaataaaa accgctaata tacgaatgac
     1441 taactatcag tagcgttatc cctatttctg gagatattcc tttgatcaac gttctacttg
     1501 ttgatgacca cgaactggtg cgcgcaggga tacgacgcat tctggaagat ataaagggta
     1561 taaaagtcgt cggtgaggca tcgtgcggtg aagacgccgt taagtggtgc cggacaaatg
     1621 ccgttgacgt ggtgctaatg gacatgagta tgccgggcat tggcggtctt gaggcgacgc
     1681 gtaaaatcgc gcgttccaca gctgatgtca aaatcatcat gcttaccgtc catacagaaa
     1741 accctttacc agcgaaagtc atgcaggccg gtgctgcggg ctacctcagc aaaggcgcgg
     1801 ctccgcagga agtcgtgagt gcgattcgtt ctgtctattc agggcagcgt tacattgctt
     1861 ctgacatcgc tcaacaaatg gcgttaagcc agatcgaacc agaaaaaaca gaaagcccat
     1921 ttgccagttt gtctgaacgt gaattgcaga ttatgctgat gatcaccaag ggccagaagg
     1981 tcaatgagat ctcagaacag ctcaatctca gtccgaaaac ggtgaacagc taccgctatc
     2041 gtatgttcag taaactaaac attcatggcg atgttgagct gactcacctg gcaattcgcc
     2101 atggtctgtg taatgcggag acattatcaa gtcagtgagt gatcagtttg acgcaaaagc
     2161 gtttttaaaa accgtaacca gccagccagg cgtttatcgc atgtacgatg ctggtggtac
     2221 ggttatctat gtcggcaaag cgaaagacct gaaaaaacgg ctttccagct atttccgtag
     2281 caacctcgct tcgcgcaaaa ccgaagcgct ggtcgcccag atccagcaaa ttgatgtaac
     2341 ggttactcac acagaaaccg aagcgctgtt gctggaacac aactacatca aactctatca
     2401 gccgcgttac aacgttttgc tacgcgatga taaatcatat ccttttatct tcctgagtgg
     2461 tgatacccac ccgcgtctgg cgatgcatcg tggtgcgaag catgccaaag gtgaatattt
     2521 cggcccgttc ccgaatggct atgccgtacg tgaaactctg gcgctactgc aaaagatttt
     2581 ccccattcgc cagtgcgaaa atagtgttta tcgcaatcgc tcgcgtccgt gtctgcaata
     2641 ccagataggg cgctgtctgg gaccgtgcgt tgaaggactg gtgagtgaag aagaatacgc
     2701 tcagcaggtc gagtatgtgc gcctgttttt gtctggcaaa gatgatcagg tgcttacgca
     2761 actcattagt cgtatggaaa ctgccagcca gaatctggag tttgaagaag ctgcacgtat
     2821 tcgcgaccaa attcaggcgg tgcgacgcgt caccgaaaaa caattcgttt ccaataccgg
     2881 cgacgacctc gacgttattg gtgtggcgtt cgatgcgggc atggcttgtg tccacgtatt
     2941 gttcattcgt cagggcaaag tgctcggcag ccgcagctat ttcccgaaag tgcctggcgg
     3001 tacggaactg agcgaggtgg tagaaacctt cgtaggccag ttctatttac aaggcagcca
     3061 gatgcgcacc ttaccgggtg agatcctgct cgattttaat cttagcgata aaacgctgct
     3121 cgccgattcc ctttcagaac tggcgggacg caagattaat gttcaaacca aacctcgcgg
     3181 cgatagggcg cgttatctga aactcgcgcg caccaatgcg gcgacggcct taaccagcaa
     3241 actttcgcag caatctaccg ttcaccagcg actgaccgcg cttgccagcg tgttgaaatt
     3301 gccggaagtg aagcggatgg agtgctttga catcagccat accatgggcg aacaaaccgt
     3361 cgcttcctgt gtggtgtttg atgctaacgg cccgctgcgt gcggagtatc ggcgctataa
     3421 cattacaggc atcacgccgg gcgatgatta tgcggcgatg aatcaggtgc tgcgtcggcg
     3481 ttatggtaaa gccattgacg acagtaagat cccggatgtg atccttatcg acggcggcaa
     3541 aggccagctt gcgcaggcga aaaatgtctt cgccgaactg gatgtctcat gggataaaaa
     3601 tcatccgctg ctacttggcg ttgccaaagg agcagatcgt aaggctggac tggaaacgct
     3661 gttctttgag ccggaaggtg agggatttag tttgccgcca gattcacccg cgctgcatgt
     3721 tatccagcat attcgcgatg aatcacatga tcacgcgatt ggcgggcacc gtaaaaaacg
     3781 ggcgaaggtc aaaaatacca gttccctgga aaccattgaa ggcgtcgggc caaaacgtcg
     3841 gcaaatgttg ttgaaatata tgggcggttt gcaaggttta cgtaacgcca gcgtcgagga
     3901 aattgcaaaa gtgccgggta tttcgcaagg tctggcagaa aagatcttct ggtcgttgaa
     3961 acattgatgt ctctgtagca acataggggt aatcttactg acaacagata gttacccgtc
     4021 attatgcaat ttaatatccc tacgttgctt acactgttcc gtgtcatcct tatcccattc
     4081 tttgtattgg tcttttatct gcctgtcacc tggtcgccgt ttgccgccgc gctcattttc
     4141 tgcgtcgcgg cggtgactga ctggttcgat ggttttctgg cacgccgctg gaaccagagt
     4201 acccggtttg gtgctttcct tgaccctgtg gcagataaag ttctcgtggc tatcgccatg
     4261 gtgctggtaa ccgagcatta tcacagctgg tgggtgacat taccggcggc aacgatgatc
     4321 gcccgtgaaa ttattatttc tgcgctacgc gaatggatgg cggagttggg taaacgcagt
     4381 agcgtggccg tctcctggat tgggaaagtg aaaaccactg cccagatggt ggcgttggca
     4441 tggctgctgt ggcgtccgaa catttgggtt gagtacgccg gtattgcact tttctttgtg
     4501 gctgcggtac tgactctgtg gtcaatgttg caatatttga gcgctgcgcg tgcagatttg
     4561 cttgatcagt gatcgtttcg gcgtaatttt cagcaaacga tcaaaagtgg tgaaaaatat
     4621 cgttgactca tcgcgccagg taagtagaat gcaacgcatc gaacggcggc actgattgcc
     4681 agacgataat aaaatcaagt gattaactga ttgcttgatg aa
//
LOCUS       DAPPNJ010000264         4721 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000264 DAPPNJ010000000
VERSION     DAPPNJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.266"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(88..384)
                     /locus_tag="RPM73_004159"
     CDS             complement(88..384)
                     /locus_tag="RPM73_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDX6522434.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            608..1327
                     /gene="tonB"
                     /locus_tag="RPM73_004160"
     CDS             608..1327
                     /gene="tonB"
                     /locus_tag="RPM73_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDX6522435.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1367..1765)
                     /gene="yciA"
                     /locus_tag="RPM73_004161"
     CDS             complement(1367..1765)
                     /gene="yciA"
                     /locus_tag="RPM73_004161"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDX6522436.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1870..2409)
                     /locus_tag="RPM73_004162"
     CDS             complement(1870..2409)
                     /locus_tag="RPM73_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDX6522437.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2439..3182)
                     /locus_tag="RPM73_004163"
     CDS             complement(2439..3182)
                     /locus_tag="RPM73_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDX6522438.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3210..3299)
                     /locus_tag="RPM73_004164"
                     /pseudo
     CDS             complement(3210..3299)
                     /locus_tag="RPM73_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3539..4177
                     /gene="ompW"
                     /locus_tag="RPM73_004165"
     CDS             3539..4177
                     /gene="ompW"
                     /locus_tag="RPM73_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDX6522439.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4237..>4721)
                     /locus_tag="RPM73_004166"
     CDS             complement(4237..>4721)
                     /locus_tag="RPM73_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDX6522440.1"
                     /translation="DWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQHLSETKNQ
                     IVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYVFEQFEIAC
                     YTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGVEAKK
                     "
BASE COUNT         1330 a         1120 c         1122 g         1149 t
ORIGIN      
        1 tggtagtgaa tgcactgacc ggctataagg aaaggccaaa caagaacacg gttgcaaaaa
       61 ccgtgccctt aaatattgaa tctctattca gaacactttc ttaaatggtt tcactgaaac
      121 gtgttcatag acacctgccg ctacgtacgg gtcagcatcg gcccaggcct gagctgcttc
      181 cagcgattca aattcagcaa taacggttga gccagtaaat cccgcagccc ctggatcgtt
      241 actgtctacc gctggcattg gaccagctgt caacaaacga ccttcatcat gcagtaactg
      301 taaacgtgct aaatgtgccg gacgaacgga aaggcgtttt tcgaggctat cagctttgtc
      361 ttgagcgtaa ataacataca acacgggcac aactccttgt tcgggaaagt tgtaaagtac
      421 gttatttgaa agggcgaaga tctgcaacgg aaagatgatg tctttgttaa gaccatgcat
      481 aaagtaaggt caattacgcc aaaaatgaca ttttcactga tcctgatcgt cttgccttat
      541 tgaatatgat tgctatttgc atttaaaatc gagacctggt ttttctactg aaatgattat
      601 gacttcaatg acccttgatt tacctcgccg cttcccctgg ccgacgttac tttcggtctg
      661 cattcatggt gctgttgtgg cgggtctgct ctatacctcg gtacatcagg ttattgaact
      721 acctgcgcct gcgcagccaa tttctgtcac gatggttgcg cctgctgatc tcgaaccgcc
      781 acaagccgtt cagccgccac cggagccggt ggtagagcca gaaccggaac ctgagccgat
      841 ccccgaaccg ccaaaagaag caccggtggt cattgaaaag ccgaagccga aacctaagcc
      901 aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa cgcgatatca aacccgtaga
      961 gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgcg cgcctgacat caagtacggc
     1021 aacggctgca accagcaagc cggttaccag tgtggcttca ggaccacgcg cattaagccg
     1081 taatcagccg cagtatccgg cacgagcaca ggcattgcgc attgaagggc aggttaaagt
     1141 taaatttgac gtcacgccgg atggtcgcgt ggataacgta caaatcctct cagccaagcc
     1201 tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc agatggcgtt atgagccggg
     1261 taagccaggc agtgggattg tggtgaatat cctgtttaaa attaacggca ccaccgaaat
     1321 tcagtaagca gaaagtcaaa agcctccgac cggaggcttt tgactattac tcaacaggta
     1381 aggcgcgagg ttttccttca ggatcaaccg cgacatactt aaataatgct tctgtcgctt
     1441 tatagcgttg cccgattggt tcagacgcta cttttttcac ccacacttca atattaatgc
     1501 tgaccgatgt cgtccctttc tggacacagc gtgcatagca gcacaccaca tcgccgaccg
     1561 caaccggccg taagaaagtc attccttcaa cccgcacagt cactacgcga ccgtgggcaa
     1621 tttctttcgc cagaatagcg ccgccaatat ccatttgtga cattaaccaa ccaccaaaga
     1681 tgtcaccatt ggcattggta tcggcgggca tggctaaagt acgtaaaaca agatcgccct
     1741 gagggacgtt atgtgttgta gacatggtaa aaccgactta aaaggaaaat cacaggcgcg
     1801 atgctactat gattttcctg aggagaacag aggagataca gcgccagccc cgaaggactg
     1861 gcagtctggt taggatttat cttcctgcgg catgtggcgg tagatataga taccgcttaa
     1921 caatgtgaag attaaggtaa gggcggtcag gccaaagact ttaaagttga cccaaatatt
     1981 ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg caaaggataa agaaaacagc
     2041 ccaagccaga tttagcttcg accataccgg ttgcggcagc gtgagttctt tacccagcat
     2101 ccgctgaatt agcggctttt tcatcaccca ttggctgact aacagggctc ccgcaaacag
     2161 agcataaatg actgtaacct tccatttaat aaactcatca ttgtggaaga acagcgtcaa
     2221 gccaccgaag acgaccacca gaacaaaagt gatcagggcc atcttctcaa ccttacgaaa
     2281 gcgaacccag ctatagataa gcacaatcgc cgtggcgacg atcagcgccg cagtagccgc
     2341 gtagatgtca taaatcttgt aaaacgcgaa aaagacaacc agcggtaaaa aatcaagaaa
     2401 ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt attggcgaat caacatatac
     2461 aggcggaaca gatagatgag caatatggca gaaatcaggt tgctcaaggt gttcgccagt
     2521 acggcaccaa tttccggggt taatgcggca aaagaagagg caaaaagcag caacagtgtt
     2581 tttgccagca accagctcag tactgcgggt gccaccagac gcatattcgc ccaagtcagc
     2641 cgcatactgc tacgcatcga ggcaaaaatg cccattttgt cctgtaccag catcaccggt
     2701 gccagggcca gtaaaatggc catgataatt cccggaacga ccaccagcat gatgccaatc
     2761 tgtactaaaa gggtagtcag aaaaatcaga ataaataact tgggcaatat cggcgcactg
     2821 gcaccaatag ctcgcagcgc actgactctc tgacccgcag acaccagctg gataattaat
     2881 attacgcctc cggcgagaat ggcgttaccg attaatcctg aaaaagtgga cgccgctgaa
     2941 gcctgcagca aaatttgttg ctgttccggt gacatattct gaaccaggtc gaacaaccca
     3001 ctactgccgc taacgggcac gccgtcattg agctgcgcaa gctgtgcatc actgggtgag
     3061 aaaacatgcc ctaacaccac tgtgataaac gcacatagca acgataccaa cagaatggtc
     3121 ataaattgat tacggaagaa atttccggtg tcacggtata cggactgcgc cgtgatagac
     3181 atgcactctc cttgagtttt atgcaggtgt taattagcgg gcaattgtac cctggttaag
     3241 cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat ctttgtaaag cggaggtaat
     3301 tgctggcctt caggcgtggg caaaccgcgg caagtggcga tcgagttaat atttgcgtag
     3361 agaaaatatt taaaaattga tttaaatcac attaaccagg attctcaatg caacttctaa
     3421 attaatccag atcaataaat ggtgaattat catatgtaat gtgatctatg taggatcatt
     3481 tgttactcca atgtaggtat attcgtcacg tttttataac cataacgacg gagcggatat
     3541 gaaaaagtta acagtggcgg ctttggcagt aacaactctt ctttctggca gtgcctttgc
     3601 gcatgaagca ggcgaatttt ttatgcgtgc aggttctgca accgtacgtc caacagaagg
     3661 tgctggtggt acgttaggaa gtctgggtgg attcagcgtg accaataaca ctcaactggg
     3721 cttgacgttt acttatatgg cgaccgacaa cattggtgtg gaattactgg cagcgacgcc
     3781 gttccgccat aaaatcggca cccgggcgac cggcgatatt gcaaccgttc accatctgcc
     3841 gccaacactg atggcgcagt ggtattttgg tgatgccagc agcaaattcc gtccttacgt
     3901 tggggccggt attaactaca ccaccttctt tgataatgga tttaacgatc atggcaaaga
     3961 ggcggggctt tccgatctca gtctgaaaga ttcctgggga gctgccgggc aggtgggggt
     4021 tgattatctg attaaccgtg actggttggt taacatgtca gtgtggtaca tggatatcga
     4081 taccaccgcc aattataagc taggcggtgc acagcaacac gatagcgtac gcctcgatcc
     4141 gtgggtgttt atgttctcag caggatatcg tttttaattc cgcacaaaaa cgaccccgta
     4201 atatacgggg tcaataagga catggtataa agggtattat ttcttcgctt ctacgccatc
     4261 agtttcagaa cgaattaaaa atttctcagt tgtttgcgga atatgctgaa tcagccagtc
     4321 ggccatttgc ttttcctcat ttaaaatcgc ttcgatgatc ggaattgaga ctgtatcacc
     4381 ggcatttttt gctgctgcta atagtgaggt gtaacaggcg atttcaaatt gctcgaagac
     4441 atatccgcta atagagcctt taactatttc atcagaaggg aatataccac cgattgactg
     4501 cccaagcgca gccattttac tcatggaatc tttaatgact gaacgtgaaa tgtcattacg
     4561 atcaagaata gtttccagtt gaacaatctg gtttttggtt tcactaagat gttgttcaat
     4621 acgagcgcgt agttcaggat aattatctat acgactggcc atggattcaa gcatagattc
     4681 ggcttgcttt tccattgcgt gggcgtcacg taaccagtca t
//
LOCUS       DAPPNJ010000265         4711 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000265 DAPPNJ010000000
VERSION     DAPPNJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.267"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1117
                     /gene="chbC"
                     /locus_tag="RPM73_004167"
     CDS             <1..1117
                     /gene="chbC"
                     /locus_tag="RPM73_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDX6522441.1"
                     /translation="IGGNVYNGTLGIMSLMAPFFIGMALAEERKVDALAAGLLSVAAF
                     MTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRNWVIKLPDSVPASV
                     SRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGSVVGWAYVIFV
                     PLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAAGKTFHIWAKPMLD
                     SFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILFGLPIIMNPVM
                     FIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGSVAALLVALFN
                     LGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1168..1518
                     /gene="chbA"
                     /locus_tag="RPM73_004168"
     CDS             1168..1518
                     /gene="chbA"
                     /locus_tag="RPM73_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDX6522442.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1526..2368
                     /gene="chbR"
                     /locus_tag="RPM73_004169"
     CDS             1526..2368
                     /gene="chbR"
                     /locus_tag="RPM73_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDX6522443.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2473..3825
                     /gene="celF"
                     /locus_tag="RPM73_004170"
                     /gene_synonym="chbF"
     CDS             2473..3825
                     /gene="celF"
                     /locus_tag="RPM73_004170"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDX6522444.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            3838..4596
                     /gene="chbG"
                     /locus_tag="RPM73_004171"
     CDS             3838..4596
                     /gene="chbG"
                     /locus_tag="RPM73_004171"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDX6522445.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
BASE COUNT         1178 a         1004 c         1259 g         1270 t
ORIGIN      
        1 tattggcggc aacgtatata acggaacatt agggataatg tctttaatgg caccgttctt
       61 tattggcatg gcgctggcag aagagcgtaa agtcgatgcg ctggcggctg ggttgttatc
      121 cgttgcagca tttatgaccg tcaccccata tagtgtcggt gaggcctatg cggttggcgc
      181 aaactggtta ggtggggcga atatcatctc cgggattatt attggcctgg tggtggcaga
      241 aatgtttacc tttattgtcc gccgcaattg ggtcattaaa ctgcccgaca gcgtacctgc
      301 ttcagtatcg cgttccttct cggcattaat tcccggcttt attattcttt ccgtgatggg
      361 gattattgcc tgggcgttga atacctgggg caccaacttc catcagatca ttatggatac
      421 catctcaacc ccactggcat cgttgggtag cgtggtgggc tgggcctatg tgatctttgt
      481 tccactgctc tggttcttcg gtattcatgg cgcgctggcg ctgaccgcac tggacaacgg
      541 cattatgacg ccgtgggcgc tggaaaatat cgcgacctat cagcaatatg gttccgtcga
      601 agcggcgctg gcagccggta agaccttcca tatctgggcc aagccgatgc tggactcctt
      661 tattttcctt gggggcagtg gtgcgacttt aggcctgatc ctggctatct ttatcgcctc
      721 tcgccgtgct gattatcgtc aggtggcaaa actggcgctg ccgtccggca tcttccagat
      781 taacgaaccg attctgtttg gtctgccaat tatcatgaac ccggtgatgt ttatcccgtt
      841 tgtactggta caaccgattc tggcggcaat caccctcgca gcgtactaca tgggcatcat
      901 tcctccggtg accaatattg caccgtggac catgccaacc ggtctgggag ccttctttaa
      961 caccaacggt agcgtcgccg cattgctggt cgcactcttc aaccttggta tcgcaacgtt
     1021 aatttatctg ccctttgttg tggtggctaa caaagcacaa aacgcgattg ataaagaaga
     1081 gagcgaagaa gatatcgcta acgccctgaa attttaatcg atgccggtac gggtaatcgt
     1141 gccggtaaga atagagagga acgatgtatg atggatctcg ataacattcc cgatacgcaa
     1201 acggaagctg aagagctgga agaagtggtg atggggctta tcatcaactc cggacaagcg
     1261 cgcagcctgg cgtatgcggc actgaaacag gcgaagcagg gcgattttgc tgcagcaaaa
     1321 gccatgatgg atcagtcacg gatggcattg aatgaagcgc atctggtaca gacgaaactg
     1381 attgaaggcg atgcgggcga aggtaagatg aaagtgagtc tggtgctggt ccacgctcag
     1441 gatcatttaa tgacgtccat gcttgcgcgt gaactgatta ctgaattaat tgagcttcat
     1501 gaaaaactga aggcataagg agtcgatgat gcagccagtg attaacgcgc cggaaattgc
     1561 cactgcccga gaacagcagt tgtttaatgg caaaaacttc catgtgttta tctataacaa
     1621 aactgagagt atcagcgggc tgcatcagca cgactattat gaatttactc tggtattaac
     1681 cgggcgttat ttccaggaga ttaacggtaa gcgcgtgtta ctggaacggg gcgattttgt
     1741 ttttattccg ttaggttcgc accatcaaag tttttatgag tttggtgcca cgcgtatatt
     1801 gaacgttggg atcagtaaac gcttttttga gcagcattac ctgccgttgt tgccttattg
     1861 ctttgtcgct tcgcaggtat accggaccaa taacgctttt ctcacctatg tggaaacagt
     1921 gatttcttca ttgaatttcc gcgaaacagg gctggaagag tttgttgaga tggttacttt
     1981 ttatgtcatt aaccgtttac gtcattaccg cgaagaacag gtgattgatg atgtaccgca
     2041 gtggctgaaa agtacggtag aaaagatgca tgataaagag cagtttagtg aatcggcgct
     2101 ggagaatatg gtgacgttgt cagccaaatc acaggaatat ttgacgcgag cgactcaacg
     2161 atattatggc aaaacaccaa tgcagattat taatgagatc cgtattaatt ttgccaaaaa
     2221 acaactggaa atgaccaact attccgtgac ggatattgcg tttgaggccg gttatagtag
     2281 cccgagtttg tttattaaaa cgtttaagaa attaacgtcc tttacgccta agagctatcg
     2341 taagaaattg actgaattta atcagtaagt cgttatacct gacaattcac atatctgtcc
     2401 tgttgctgga ttatttatgt ccgggggggc agatatgcca gatatcagta ttctgtactg
     2461 aagggagaaa ttatgagcca gaaattaaaa gtcgtcacta ttggtggcgg gagcagctat
     2521 accccggagt tactggaagg atttattaag cgttatcacg aattgccggt cagcgaatta
     2581 tggctggtgg atgtcgaaga tggtaaagag aaactggata tcatttttga actctgccaa
     2641 cggatgattg ataacgctgg cgtcccgatg aagctttata aaacgctgga tcgccgcgaa
     2701 gcattgaaag atgctgattt cgttactacc caactgcgcg taggccaatt accggcgcgc
     2761 gaactggatg aacgtattcc attaagtcat ggttatcttg gtcaggaaac caacggcgcg
     2821 ggcggtctgt ttaaaggtct gcgtaccatt ccggtgattt ttgacatcgt aaaagatgtc
     2881 gaagaacttt gtccgaatgc atgggtgatt aacttcacta acccggcggg aatggtcact
     2941 gaagccgttt atcgtcatac cggatttaaa cgctttatcg gcgtgtgtaa tattccgatc
     3001 ggcatgaaga tgtttattcg cgatgttctg atgctgaaag acagcgatga tttatctatc
     3061 gatctgttcg gcctcaacca tatggtgttc attaaggatg tgctggtaaa tggcaagtcg
     3121 cgctttgccg aattgcttga tggtgtggcg tcagggcagt taaaagcgtc ctctgtaaaa
     3181 aatattttcg atctgccatt tagtgagggc ttaattcgtt cgttaaatct gctgccatgt
     3241 tcttatctgc tgtattactt caagcagaaa gagatgctgg ctattgaaat gggcgaatac
     3301 tacaaaggcg gcgcacgagc acaggtagta cagaaagtcg agaaacaact ttttgagctg
     3361 tataaaaatc cggagttgaa agttaagccg aaagaactgg aacagcgcgg tggggcttat
     3421 tactctgatg cagcgtgcga agtgatcaac gctatctaca acgacaagca agctgaacat
     3481 tacgttaata tcccgcatca tgggcatatt gataatattc cggcagactg ggcggtagaa
     3541 atgacctgta agctggggcg cgatggcgcg acgccacatc cgcgcattac gcatttcgat
     3601 gataaagtga tggggctgat tcacaccatt aaaggcttcg agattgctgc cagcaacgcc
     3661 gcacttagcg gagaatttaa cgatgtgtta ctggcgctaa accttagtcc gttggtgcat
     3721 tccgatcgcg atgctgagct gctggcacgc gagatgattc tggcgcacga gaaatggctg
     3781 ccaaattttg ccgactgcat cgcagagctt aaaaaagcac attaaccgag gctgattatg
     3841 gaacgcttac tgattgttaa tgccgatgat tttggcttaa gcaaaggtca gaactacggc
     3901 attatcgagg cctgtcgcaa tgggattgtc acatcaacga cggcgctggt gaatgggcag
     3961 gctattgacc atgcggtgca gttgagtcgt gatgaaccga gtctggccat agggatgcac
     4021 tttgtcctta ctatgggcaa gccactgaca gctatgccgg ggttaacccg cgatggtgtg
     4081 ctgggaaaat ggatctggca gttggcagaa gaagatgctt taccgctgga agaaattact
     4141 caggagcttg ccagtcagta tttgcgtttc attgagctat ttggacgcaa acctacgcat
     4201 cttgatagcc atcatcatgt gcatatgttc ccgcagattt tcccgattgt tgccaggttt
     4261 gcggctgaag aggggattgc gttgcgcatc gaccgtcagc cgctatctaa cgctggtgat
     4321 ttaccggcta atctgcgcag ttctcaggga ttcagtagcg cgttttatgg tgaagagatt
     4381 agcgaggcac tgttcctgca agtgctggat gacgcaagcc atcggggtga tcgttcgctg
     4441 gaggtgatgt gtcatccggc gtttatcgat aatactatcc gtcagagtgc ttactgtttc
     4501 ccacgcttaa cggagctgga tgtgttgaca tcagcgtcgt tgaaaggtgc tatcgctcag
     4561 cgtggttatc ggttggggag ttatcgggat gtgtaagtga ggatgtttgc cagatgcggc
     4621 gtgaaccttg catccggcga ttaattccta caaaggcggc gtaactgcgc cgcttgagac
     4681 tgctgacaaa cgcaaaactg cctgatgcgc t
//
LOCUS       DAPPNJ010000266         4700 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000266 DAPPNJ010000000
VERSION     DAPPNJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.268"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            62..1024
                     /gene="tauA"
                     /locus_tag="RPM73_004172"
     CDS             62..1024
                     /gene="tauA"
                     /locus_tag="RPM73_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX6522446.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1037..1804
                     /gene="tauB"
                     /locus_tag="RPM73_004173"
     CDS             1037..1804
                     /gene="tauB"
                     /locus_tag="RPM73_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDX6522447.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1801..2628
                     /gene="tauC"
                     /locus_tag="RPM73_004174"
     CDS             1801..2628
                     /gene="tauC"
                     /locus_tag="RPM73_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDX6522448.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2625..3476
                     /gene="tauD"
                     /locus_tag="RPM73_004175"
     CDS             2625..3476
                     /gene="tauD"
                     /locus_tag="RPM73_004175"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDX6522449.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3582..4556)
                     /gene="hemB"
                     /locus_tag="RPM73_004176"
     CDS             complement(3582..4556)
                     /gene="hemB"
                     /locus_tag="RPM73_004176"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDX6522450.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1049 a         1216 c         1374 g         1061 t
ORIGIN      
        1 tttaatgaat gtttttattc ctgaatactg ctcccataac aagacagggg agcagacaat
       61 catggcaatt tcatcgcgta acacacttct tgccgcactg gcattcatcg cttttcaggc
      121 acaggcggtg aacgtcaccg tggcgtatca aacctcagcc gaaccggcga aagtggctca
      181 ggccgacaac acctttgcta aagaaagcgg agcaaccgtg gactggcgta agtttgacag
      241 cggagccagc atcgtgcggg cgctggcttc tggcgacgtg caaatcggca acctcggttc
      301 cagcccatta gcggttgcag ccagccaaca ggtgccgatt gaagtcttct tgctggcgtc
      361 aaaactgggt aactccgaag cgctggtggt aaagaaaact atcagcaaac ccgaagattt
      421 gattggcaaa cgcattgccg taccgtttat ctccaccacc cactacagcc tgctggcggc
      481 gctgaaacac tgggggatta aaccagggca agtggagatt gtgaacctgc agccgcccgc
      541 gattatcgcc gcatggcagc ggggtgatat tgatggtgct tatgtctggg caccggcggt
      601 taacgccctg gaaaaagacg gcaaggtgtt gaccgattct gaacaggtcg ggcagtgggg
      661 tgcgccaacg ctggacgtct gggtggtgcg caaagatttt gccgagaaac atcctgaggt
      721 cgtgaaagcg ttcgctaaaa gcgccatcga tgctcagcaa ccgtacattg ctaacccaga
      781 cgcgtggctg aaacagccgg aaaacatcag caaactggcg cgtttaagcg gcgtgccgga
      841 aggtgacgtt ccggggctgg tgaaggggaa tacctatctg acgccgcagc aacaaacggt
      901 agaactgacc ggaccggtga acaaagcgat catcgacacc gcgcagtttt tgaaagagca
      961 gggcaaggtc ccggctgtag cgaatgatta cagccagtac gttacctcgc gcttcgtgca
     1021 ataaaaggag gcgcagatgc tgcaaatctc tcatctttac gccgattatg gcggcaaacc
     1081 ggtactggaa gatatcaacc tgacgctgga aagcggcgag ctactggtgg tgctggggcc
     1141 gtccggctgc ggtaaaacca ccctgctgaa tctgattgcc ggttttgtgc cttatcagca
     1201 tggcagcatt caactggcgg gtaagcgtat tgagggaccg ggagcagagc gcggggtggt
     1261 ttttcagaat gaagggctac taccgtggcg caatgtacag gacaacgtgg cgttcggcct
     1321 gcaattggca ggtatagaga aaatgcagcg actggaaatc gcgcaccaga tgctgaaaaa
     1381 agtggggctg gaaggcgcag aaaaacgcta tatctggcag ctttccggtg gtcaacgtca
     1441 gcgggtgggg attgcccgcg cgctggcggc gaatccccag ctgttattac tcgacgaacc
     1501 gtttggtgcg ctggacgcct tcacccgcga ccagatgcaa actctgctgc tgaaactctg
     1561 gcaggagacg ggcaagcagg tgctgttgat tacccacgat atagaagaag cggtgtttat
     1621 ggcgactgaa ctggttctgc tttcatccgg ccctggccgt gtgctggagc ggctgccgct
     1681 caactttgct cgccgctttg ttgcgggaga gtcgagccgc agcatcaagt ccgatccaca
     1741 attcatcgcc atgcgcgaat atgttttaag ccgcgtattt gagcaacggg aggcgttctc
     1801 atgagtgtgc tcattaatga aaaactgcat tcgcggcggc tgaaatggcg ctggccgctc
     1861 tcgcgtcagg tgaccttaag cattggcacg ttagcggttt tactcactgt atggtggacg
     1921 gtggcggcgc tgcaactgat tagcccgctg tttttgccgc cgccgcaaca agtactggca
     1981 aaactgctca ccattgccgg accgcaggga tttatggacg ccacgctgtg gcagcatctg
     2041 gcagccagcc tgacgcgcat tgtgctggcg ctactcgcgg cggtggtgat cggtattccg
     2101 gtcgggatcg ccatggggct aagcccgacg gtgcgcggca ttctggatcc ggtaatcgaa
     2161 ctttatcgtc ccgtgccgcc gctggcttat ttgccgctga tggtgatctg gtttggtatt
     2221 ggtgaaacct cgaagatctt actgatctat ttagcgattt ttgcaccggt ggcgatgtcg
     2281 gcgctggcgg gggtgaaaag cgcgcagcag gttcgcattc gtgccgccca gtcgctgggt
     2341 gccagccgtg cgcaggtgct gtggtttgtc attttgcccg gcgcgctgcc ggaaatcctc
     2401 accggattac gcattggtct gggtgtgggc tggtctacgc tggtagcggc ggagctgatt
     2461 gccgcgacgc gcggtttagg atttatggtt cagtcagcgg gtgaatttct cgcgactgac
     2521 gtggtgctgg cgggaatcgc ggtgattgcg attatcgcct ttcttttaga actaggtttg
     2581 cgcgcgttac agcgccgtct gacgccctgg catggagaag tacaatgagt gaacgtctga
     2641 gcattacccc gctggggccg tatatcggcg cacaaatttc gggtgccgac ctgacgcgcc
     2701 cgttaagcga taatcagttt gaacagcttt accatgcggt gctgcgccat caggtggtgt
     2761 ttctgcgcga tcaaactatt acgccgcagc agcaacgcgc gctggcccag cgttttggcg
     2821 aactgcatat tcaccctgtt taccctcatg ccgaaggggt tgacgagatc atcgtgctgg
     2881 atacccataa cgataatccg ccggataacg acaactggca taccgatgtg acatttattg
     2941 aaacgccacc cgcaggggcg attctggcag ctaaagagtt accttcgacc ggcggtgata
     3001 cgctctggac cagcggtatt gcggcctatg aggcgctctc tgttcccttc cgccagctgc
     3061 tgagcgggct gcgtgcggag catgatttcc gtaaatcgtt cccggaatac aaataccgca
     3121 aaaccgagga ggaacatcaa cgctggcgcg aggcggtcgc gaaaaatcct ccgttgctac
     3181 atccggtggt gcgaacgcat ccggtgagcg gtaaacaggc gctgtttgtg aatgaaggct
     3241 ttactacgcg aattgtcgat gtcagcgaga aagagagcga agccttgtta ggctttttat
     3301 tcgcccatat cactaaaccg gagtttcagg tgcgttggcg ctggcaaccg aatgatattg
     3361 cgatttggga taaccgcgtg actcagcact atgccaatgc cgattacctg ccacagcggc
     3421 ggattatgca tcgggcgacg attcttgggg ataaaccgtt ttatcgggcg gggtgatacg
     3481 agtgcggacg gttccctcgc ccccttgggg agagggttag ggtgaggggg cgttcaccgt
     3541 actttcaaca ggttaactcc ccctttctga gaggaaaaaa attaacgcag aatcttcttc
     3601 tcagccaaat ccagcgcaaa gtagctgaaa atcagatccg cacccgcacg cttaattgaa
     3661 cctaagcttt cgagcacgac tttctcttca tctatagcac ccgccagcgc ggcgaactta
     3721 atcatcgcgt actcaccgct cacctgatac gcgccaatcg gcaattcagt acgttcacgc
     3781 agctcacgca cgatgtcgag gtatgctcca gcaggtttaa ccatcaggca gtctgcgccc
     3841 tgggcttcat ccagcagtga ttcacgaatc gcctcgcgac ggttcattgg gttcatctga
     3901 tagcttttgc ggtcgccttt taatgcgctt ccggcagctt cacggaacgg accataaaag
     3961 gaagaggcga acttggtcga atacgacata atcgccgtat ctttaaagcc tgcagcgtcc
     4021 agcgcctgac gaatcgcctg tacctggccg tccatcgcgg cggaaggggc gatgaagtct
     4081 gcgcctgcag cagctgcaac cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg
     4141 tcgacgccat gctcgcacag cacaccgcag tgaccgtgag aagtgtattc acagaaacaa
     4201 gtgtctgaca tgacgatcat ttctggcaca gtctgcttgc agatgcgcga catacgcgcc
     4261 accagtccat cttcccgcca ggcgtcgctg ccggtttcat cggtatggtg agagatgcca
     4321 aaagtcatta cggaacggat accggcgttg gcgatgcgtt caatttcgcg tgccagatgt
     4381 ttctctggaa tgcgcatcac gcctggcatg gcttcaacgg ctttgtagtc gtcaatttct
     4441 tcttcaacaa agatcggcaa caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata
     4501 gcgcgcagcg caggagattt gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc
     4561 tgcctgatgt ttgtggaatt aaggtgcaca gtatacctga agcggggcag gagtgtttta
     4621 cgaaagttgt cgtgatgttg acaagaagag aatgaaagag agaccaggac atctttgtaa
     4681 tagaaatagc aataatttca
//
LOCUS       DAPPNJ010000267         4615 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000267 DAPPNJ010000000
VERSION     DAPPNJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.269"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            334..4587
                     /gene="fdeC"
                     /locus_tag="RPM73_004177"
     CDS             334..4587
                     /gene="fdeC"
                     /locus_tag="RPM73_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDX6522451.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSDVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1222 a         1070 c         1292 g         1031 t
ORIGIN      
        1 atagttgtta attaaaggca ggccattatt attcactctg aataatgaat tattcactgt
       61 ccgcagagta aaaaatataa cttaggtgtc tatttaatga cttgcacaaa aagctaaatt
      121 ttcacccata aataaaaata taatcccgcg cccaaccacc tgatgagtgg ctacagacac
      181 tggatatatt aggtggcggt gcactttctt acataaaggt atttcctttt ctgcggaaaa
      241 ggaaatcggg aaatccccgg tttttctgac aagcagacgc cattatttgt gtctgcctat
      301 gttcgttaat tcgttcatca ggaaattatc tcaatgtcgc gttataaaac aggtcataaa
      361 caaccacgat ttcgttattc agttctggcc cgctgcgtgg cgtgggcaaa tatctctgtt
      421 caggttcttt ttccactcgc tgtcaccttt accccagtaa tggcggcacg tgcgcagcat
      481 gcggttcagc cacggttgag catgggaaat actacggtaa ctgctgataa taacgtggag
      541 aaaaatgtcg cgtcgtttgc cgcaaatgcc gggacatttt taagcagtca gccagatagc
      601 gatgcgacac gtaactttat taccggaatg gccacagcta aagctaacca ggaaatacag
      661 gagtggctcg ggaaatatgg tactgcgcgc gtcaaactga atgtcgataa agatttctcg
      721 ctgaaggatt cttcgctgga aatgctttat ccgatttatg atacgccaac aaatatgttg
      781 ttcactcagg gagcaataca tcgtaccgac gatcgtactc agtcaaatat tggttttggc
      841 tggcgtcatt tttcaggaaa tgactggatg gcgggggtga acacctttat cgaccatgat
      901 ttatcccgta gtcatacccg cattggtgtt ggtgcggaat actggcgcga ttatctgaaa
      961 ctgagcgcca atggttatat ccgggcttct ggctggaaaa aatcgccgga tattgaggat
     1021 tatcaggaac gcccggcgaa tggttgggat atccgcgcag agggctattt acctgcctgg
     1081 ccgcagcttg gcgcaagcct gatgtatgaa cagtattatg gcgatgaagt cgggctgttt
     1141 ggtaaagata agcgccagaa agacccgcat gctatttctg ccgaggtgac ctatacgcca
     1201 gtgcctcttc tgacactgag cgccgggcat aagcagggca agagcggtga gaatgacact
     1261 cgctttggcc tggaagttaa ttatcggatt ggcgaacctc tggagaaaca actcgataca
     1321 gacagcattc gcgagcgtcg aatgctggca ggcagccgct atgacctggt tgagcgtaat
     1381 aacaacatcg ttcttgagta ccgcaaatct gaagtgatcc gtattgctct gcctgagcgt
     1441 attgaaggta agggcggtca gacactttcc ctggggcttg tggtcagcaa agcaactcac
     1501 ggactgaaaa atgtgcagtg ggaagcgccg tcattactgg ctgaaggtgg caaaattacc
     1561 ggtcagggta gtcagtggca agtaacgctc ccggcttatc gtccaggcaa agacaattat
     1621 tatgcgattt ctgcggttgc ctacgataac aaaggcaatg cctcaaaacg cgtgcagaca
     1681 gaggtggtca ttaccggagc aggtatgagt gccgatcgca cggcgttaac gcttgacggt
     1741 cagagccgta ttcaaatgct tgctaacggt aatgagcaaa gaccgctggt gctgtctctg
     1801 cgcgacgccg agggccagcc agtcacgggc atgaaagatc agatcaagac tgaactaact
     1861 ttcaaaccgg ctggaaatat tgtgactcgt accctgaagg ccactaaatc acaggcaaag
     1921 ccaacactgg gtgagttcac cgaaactgaa gcaggggtgt atcagtctgt ctttactacc
     1981 ggaacgcagt caggtgaggc aacgattact gttagtgttg atggcatgag caaaaccgtc
     2041 actgcagaac tgcgggccac gatgatggat gtggcaaact ccaccctgag cgctaacgag
     2101 ccgtcaggtg acgtggttgc tgatggtcag caagcctaca cgctgacgct gactgcggtg
     2161 gatactgatg gtaacccggt gacgggagag gccagccgct tgcgatttgt tccgcaagac
     2221 actaatggtg tcaccattgg tacaatttcg gagataaaac caggcgttta cagcgctacg
     2281 gtttcttcga cccgtgccgg aaacgttgtt gtgcgtgctt tcagcgagca gtatcagctg
     2341 ggcacattac aacaaacgct gaagtttgtt gccgggccgc ttgatgcagc acattcgtcc
     2401 atcaccctga atcctgataa accggtggtt gggggtacag ttacggcaat ctggacggta
     2461 aaagatgcct atgacaaccc tgtgaccagc ctcacgccgg aagcgccgtc attagcgggt
     2521 gccgctgctg taggttctac ggcatcgggc tggacaaata atggtgatgg gacgtggact
     2581 gcgcagatta ctctcggctc tacggcgggt gaattagaag ttatgccgaa gctaaatgga
     2641 caggatgcgg cagcaaatgc ggcaaaagta accgtggtgg ctgatgcgtt atcttcaaac
     2701 cagtcgaaag tctctgtcgc agaagatcac gtaaaagccg gcgaaagtac aaccgtgacg
     2761 ctggtggcga aagatgcgca tggcaacgct atcagtggtc tttcgttgtc ggcaagtttg
     2821 acggggaccg cctctgaagg ggcgaccgtt tccagttgga ccgaaaaagg tgacggttcc
     2881 tatgttgcta cgttgactac aggcggaaag acgggcgagc ttcgtgtcat gccgctcttc
     2941 aacggccagc cagcagccac cgaagccgcg cagttgacgg tcatcgccgg agagatgtca
     3001 tcagcgaact ctacgcttgt tgcggacaat aaggctccga ccgtcaaaac gacgacggaa
     3061 ctcaccttca ccgtgaagga tgcgtacggg aacccggtca ccgggctgaa gccagatgca
     3121 ccagtgttta gcggtgccgc cagcacgggg agtgagcgtc cttcagcagg aagctggaca
     3181 gagaaaggta atggggtcta cgtgtcgacc ttaacgctgg gatctgccgc gggtcagttg
     3241 tctgtgatgc cgagagtgaa cggccaaaat gccgttgctc agccactggt gctgaacgtt
     3301 gcaggtgacg catctaaggc tgagattggt gatatgacag tgaaggttaa taaccaactg
     3361 gctaatggac agtctgctaa ccaggtaacc ctgaccgttg tggacaccta tggtaacccg
     3421 ttgcagggac aaaatgtgac gctgactctg ccgaaaggtg tgaccagcaa gacggggaat
     3481 acggtaacaa ccgatgcggc aggtaaagcc gacattgagc tgatgtcaac ggttgccggg
     3541 gaacacagca tcacggcctc agtgaataat gctcagaaga cggttacggt gaaattcaag
     3601 gcggatttca gtaccggtca ggcgagtctg gaggttgata gcgccgcgcc aaaagtagca
     3661 aacggcaaag atgcctttac gctgacggcg accgttgagg ataaaaatgg taaccctgtt
     3721 ccagggagcc tggtgacctt taatctgccc cggggtgtca agccgcttac aggcgataat
     3781 gtctgggtga aagccaacga tgaggggaaa gcagagttgc aggtggtttc agtgactgcc
     3841 ggaacgtatg agatcacggc atcggcgggg aatagccagc cttcgaatac gcagactata
     3901 acgtttgtag ccgataaggc taccgcaacc gtctccggta ttgaggtgat tggcaactat
     3961 gcgctggcgg acggcaaagc caaacaaacg tataaagtta cggtgactga tgccaataac
     4021 aatttggtga aagatagcga cgtgacgctg actgccagcc cggcttcgtt aaacctggaa
     4081 ccgaatggca ctgcgacaac gaatgagcaa gggcaggcta ttttcaccgc taccactact
     4141 gttgcggcga catacacact caaggcgcaa gtgagtcaga ccaacggtca ggtatcaacg
     4201 aaaactgccg aatctaaatt cgttgcggat gataaaaacg cggtactcac cgcatcatct
     4261 gatatgcaat ctctggtggc ggatgggaaa tcgactgcga agctggaggt gacactgatg
     4321 tcggcaaaca accccgttgg cgggaatatg tgggtcgaca ttcagacgcc ggaaggggtg
     4381 acggagaagg attatcagtt cctgccgtcg aaaaatgacc atttcgtgag cggaaaaatc
     4441 acgcgtaaat ttagtaccag caagcctggt gtctatacgt tcacatttaa cgccctgacc
     4501 tatggcgggt acgaaatgaa gccagtgacg gtgaccatta ccgcggtgga tgccgatacg
     4561 gcaaaggacg aggaggcgat gaaataattt taagaatatg aaagtaacat tctaa
//
LOCUS       DAPPNJ010000268         4595 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000268 DAPPNJ010000000
VERSION     DAPPNJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.270"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..2403
                     /locus_tag="RPM73_004178"
     CDS             <1..2403
                     /locus_tag="RPM73_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6522452.1"
                     /translation="EFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNALINNKRTPI
                     HLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDK
                     GKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISS
                     YGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDT
                     ESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVP
                     PGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTS
                     VGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDV
                     TRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHY
                     TNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDTSSNVNYSFSLSRDFDIGPLKN
                     VSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDR
                     NNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFT
                     ATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLS
                     VDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVK
                     DKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCIS
                     QN"
     gene            2419..3168
                     /locus_tag="RPM73_004179"
     CDS             2419..3168
                     /locus_tag="RPM73_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX6522453.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVISYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3170..4234
                     /locus_tag="RPM73_004180"
     CDS             3170..4234
                     /locus_tag="RPM73_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522454.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSAYP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4277..4477)
                     /gene="glgS"
                     /locus_tag="RPM73_004181"
     CDS             complement(4277..4477)
                     /gene="glgS"
                     /locus_tag="RPM73_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDX6522455.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         1419 a          960 c          922 g         1294 t
ORIGIN      
        1 gaattcaaca aagatttaat cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt
       61 gaaactgatg gccaattacc cgtaggtaaa tactcattaa acgctcttat taataataaa
      121 aggacgccaa tccatctgga cctgcaatgg gtattaattg ataaccaaac tgcagtttgt
      181 ctgactccag agcaattaac attattaggc tttactgatg agattattga agtggctcag
      241 caaaacctga tcgatggttg ttaccctatc gaaaaagaaa aacaaattac aacttatctc
      301 gataaaggga aaatgcaatt atccatatct gcacctcagg catggttaaa atacaaagat
      361 gcaaactgga cgcctcctga actttgggat catggtattg ctggggcatt tcttgactac
      421 aatttatatg cctctcatta tgcaccacat cagggcgata attcacaaaa tataagttcc
      481 tatgggcagg ctggggttaa tcttggggcc tggcgcctgc gtactgatta ccagtacgat
      541 caatcattta acaatggcaa aagccaggcg aacaacctgg attttccgcg tatttatttg
      601 tttcgcccaa tcccagcaat taatgcaaaa ctaactatag gtcaatacga tactgaatcc
      661 tctattttcg actctttcca tttttctggc gtttcgttga aaagcgatga gaatatgttg
      721 ccgcctgatc tacgtggtta cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag
      781 gtcactgtct cacagaacaa ccgtattatt tatcaagaaa atgttcctcc tggcccattt
      841 gctattacta atttattcaa tacattacag gggcaactgg acgtcaaggt tgaagaagag
      901 gacgggcaag tcacgcaatg gcaagttgca tctaatagta ttccttatct gacgcgtaaa
      961 gggcagattc gctacaccac tgctatgggt aaaccgacca gcgttggtgg tgattcctta
     1021 caacaaccct tcttctggac tggtgaattc tcatggggtt ggctgaacaa tgtatccctg
     1081 tatggtggtt cagttttaac aaaccgtgat tatcaatctc tggctgccgg cgttggcttt
     1141 aaccttaact cattgggttc attatctttt gatgtcacac gatctgatgc tcagttgcat
     1201 aatcaggata aagaaacggg ttatagctac cgcgctaact attcaaaacg ttttgaatct
     1261 accggtagcc agctcacttt cgctggttac cgtttctctg ataaaaactt tgtgacaatg
     1321 aatgaatata tcaatgacac taaccattac acgaattatc agaatgaaaa agagagttat
     1381 attgtcacgt ttaaccagta tcttgaatca ttaaggttaa atacatacgt aagtttggct
     1441 cgtaatactt actgggacac cagcagtaat gtgaattatt cattttcact tagccgcgat
     1501 tttgatatcg ggccattaaa aaacgtctcc acttctctaa catttagccg aataaactgg
     1561 gaagaagaca accaggatca actgtaccta aatatttcga ttccctgggg aaccagtaga
     1621 acattgagct atggtatgca acgaaatcag gataacaaga tttcgcatac tgcttcgtgg
     1681 tatgactctt ccgatcgaaa taattcctgg agcgtttctg cttcaggcga caatgatgaa
     1741 ttcaaagata tgaaagcgtc actacgcgcc agttatcagc ataataccga gaacggtcgt
     1801 ctctatctct ccgggacatc acagcgagac agttattatt ctctgaatgc cagttggaat
     1861 ggttcattca cggcgactcg tcacggagcc gctttccatg actatagtgg tagtgctgac
     1921 tcgcgtttta tgatcgacgc agatggtgca gaagatattc cgttgaacaa taaacgcgcg
     1981 gtaactaatc ggtatggcat cggagttatt ccatcagtca gcagttacat aacaacatca
     2041 ttaagcgttg atacccgaaa tcttccagaa aatgtggata tcgaaaactc ggttatcacc
     2101 accaccttaa ccgaaggtgc tattggctac gccaaacttg atacccgcaa gggctatcaa
     2161 atcatggggg ttattcgcct ggcagatggt agtcacccac cactggggat tagcgtaaaa
     2221 gataaaacca gccataaaga attaggactg gttgctgatg gcggctttgt atacctcaac
     2281 ggcattcagg atgacagcaa acttacttta cgctggggtg ataaatcctg ttttattcaa
     2341 ccacccaata gcagcaactt aaccaccgga acggttattt taccgtgtat tagccaaaat
     2401 taattcagga tgaaactaat gcgctactta aacacaaaga atctcattgc agcaggagta
     2461 ttactcgcct gcatgagcag cattgcctgg ggtgccatca tcccggaccg tacccgtatc
     2521 ataatgaatg agtcagataa aggggaagca ttaaaactta ctaatcaaag caaaaatcta
     2581 ccctatctgg cgcaaacatg gatagaagat actaaaggca ataaatcacg tgactttatt
     2641 gtcacggttc cccctatggt acgtttaaat cccagtgagc aaattcaaat aagaatgata
     2701 agtcaggaaa aaattgctca actgcctaat gacagagaaa cgctcttcta ttttaacgtg
     2761 cgagaaatac cgccaaaaac agataaaaaa aatgtgatgc aagtcactat gcagcacgca
     2821 ttgaaactat tctggcgacc aaaagctatt gaattagaag atgatggggt aatttcttac
     2881 gaaaaagttg aaatcatccg tagaaatgat ggttcgatcc gctttaacaa taagatgcct
     2941 tatcacgtta cgctgggtta tatcggaaca aacggaatca caatgttgcc acaaacgcaa
     3001 agtttaatgg taacccctta tagccatgca gatacgcagt ttaaaaatgt tccgtcagct
     3061 tttcaggttg gttatatcaa tgattttggt ggattaagtt tttatgaaat aaactgcccg
     3121 acggttaata actcttgtaa tgtttccgta gccaaacgag ataaataata tgcgctactt
     3181 attaattgta attatattta ttataggttt tagtgcatta cctgtatggg caatggactg
     3241 ctatgctgaa catgaagggg gaaataccgt agtcattggt tacgtaccaa gaatttctat
     3301 ccccagtaat ggtaaaaaag gtgataagat ctggcaaagc agtgagtatt ttatgaatgt
     3361 cttctgtaat aatgcattac ctgccccatc acctggagaa gaatacccat cagcctggac
     3421 taatataatg atgtttttac caggcggtca ggacttttat aatcagaact cctatatttt
     3481 tggcgtaaca tacaatggag ttgattatga ttcgactgcc cctaatgcta tcgcagcacc
     3541 agaatgcatt gatataaaag gagcgggtac ctttaataat cactataaaa accccgctgt
     3601 ctgtagtgga ggtcctgagc ctcaattatc agtaaccttt ccagctcgcg tacaactcta
     3661 tattaaatta gcaaaaaatg ccaatagagt taataaaaac ctggtgttac ctgatgaata
     3721 tatcgctcta gagtttaaag gtatgagtgg cgcaggagct atagaagtag acaaaaattt
     3781 gaccttcagg attcgtggat taaataatat tcatgtcctt gactgctttg ttaatgtcgc
     3841 actggaacca gctgatggcg ttgttgactt tggtaaaata aattcccgaa caattaaaaa
     3901 taccagcgtt agtgagacgt ttagtgtagt catgacaaaa gatcctggtg cggcctgtac
     3961 tgagcaattc aatattttag ggagcttttt cactacggat attttgagtg attatagcca
     4021 tctggatatg ggtaatggac tgctattgaa gatatttcat aaagatggaa cagcaacgga
     4081 atttaaccgc ttctcacaat ttgcttcttt ttcatcgtct agtgcacctt cggtcaccgc
     4141 accattcagg gcagaactta gtgcgtatcc ggcagagact gttgttgagg gaccgtttag
     4201 taaagacgtc atcctcaaaa tcacctataa ctagcatcta atacaaacac taaaacgggc
     4261 catcaggccc gttatatcag tgctctaact ccagctctct tgcctgcatc atctgttgac
     4321 aataccaggc ataacgtgcg caaaaccagg ttctatgttc ttcatccatg ttaccagtaa
     4381 cggcaagaat atcgaccggg cgaccttctg cgtgcattct ggcaaaacta cgcgccagga
     4441 aatcgaaatt atttaaagaa ttaagactat gatccataag cacatcctcc ggttatcgca
     4501 taccttttaa ggccatatgc tttttctgat ttcagtattc cccaatcagt gtagtgcctt
     4561 taaacataac gtgcgtaaat atattgtccc cgatc
//
LOCUS       DAPPNJ010000269         4563 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000269 DAPPNJ010000000
VERSION     DAPPNJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.271"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..351
                     /locus_tag="RPM73_004182"
     CDS             <1..351
                     /locus_tag="RPM73_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HDX6522456.1"
                     /translation="AKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            368..715
                     /locus_tag="RPM73_004183"
                     /pseudo
     CDS             368..715
                     /locus_tag="RPM73_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            727..1773
                     /gene="fbpC"
                     /locus_tag="RPM73_004184"
     CDS             727..1773
                     /gene="fbpC"
                     /locus_tag="RPM73_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDX6522457.1"
                     /translation="MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVPRAELKARVKEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVR
                     PEAITLSDRGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYL
                     EIHPYGMFVLADAA"
     gene            complement(1882..2814)
                     /gene="mmuM"
                     /locus_tag="RPM73_004185"
     CDS             complement(1882..2814)
                     /gene="mmuM"
                     /locus_tag="RPM73_004185"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HDX6522458.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(2801..4204)
                     /gene="mmuP"
                     /locus_tag="RPM73_004186"
     CDS             complement(2801..4204)
                     /gene="mmuP"
                     /locus_tag="RPM73_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HDX6522459.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            4412..>4563
                     /locus_tag="RPM73_004187"
     CDS             4412..>4563
                     /locus_tag="RPM73_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDX6522460.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMV"
BASE COUNT         1104 a         1337 c         1211 g          911 t
ORIGIN      
        1 gctaaatcgc gccagcgctg gctgttttac gcgtatgaca ggctccggaa gacggttgtt
       61 gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca
      121 ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag
      181 ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat
      241 ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag
      301 ctgcatgaca aagtcattgg gcattatctg aacataaaac actatcaata agttggagtc
      361 attaccgggc caaatcgata aatcgctcga tgaagcctca ctcagcctgc gcgccggttc
      421 cctgcgtacc attacgcaca tcctgctgcc gctgctgcgc ccggcgatcc tctcggcgct
      481 gatttacagc tttgtgcgcg ccattaccac cgtcagcgcc attgtgttcc tcgtcacgcc
      541 ggatacccgc gtagcgacgg cttacatcct caaccgcgtg gaagatggcg aatacggtgt
      601 agcgattgcc tacggttcca ttctgatcgt ggtgatgctg gcgattattt tcatctttga
      661 ctggctgatc ggtgaatcac gtacctcccg ttcaaaagcc aaaaaccagg cgtaatggag
      721 cgcactatga ctcagaaaaa tttcgttgaa ctgcgcaacg tcactaaacg atttggcagt
      781 aatacggtaa tcgacaatat caacctcacc atcccgcagg ggcaaatggt gacgctgctc
      841 ggcccgtccg gctgcggcaa aaccactatt ttgcgcctgg ttgccgggct ggaaaaaccg
      901 agcgaagggc aaattttcat tgatggcgaa gacgtcaccc atcgctctat tcagcagcgc
      961 gatatctgta tggtgtttca gtcctatgcc ctgttcccgc atatgtcgct gggagagaat
     1021 gtcggttatg gcctgaaaat gctcggcgta ccgcgcgcag agctgaaagc ccgcgtcaaa
     1081 gaggcgttgg cgatggtgga tctggaagga ttcgaagacc gctttgtcga tcagatctcc
     1141 ggcgggcagc agcagcgcgt ggcgctggcc cgcgcgctga tcctcaagcc gaaagtgctg
     1201 ctgtttgatg agccgttgag taacctcgac gccaacctgc gtcgcagcat gcgcgacaag
     1261 atccgcgagt tgcaaaagca gtttgatatc acctcgctgt acgtcaccca cgatcagagc
     1321 gaagcctttg cggtttctga tactgtgctg gtgatgaaca agggacacat catgcagatc
     1381 ggctcaccgc aggatcttta ccgccagccc gcctcccgct ttatggcgag ctttatgggc
     1441 gatgccaacc tgttcccggc aaccttcagc gacggatacg ttgatatcta cggctatcat
     1501 ctgccgcgcc cgctgcactt tggtacacag ggtgaaggga tggtcggtgt gcgcccggaa
     1561 gcgatcacgc tcagcgatcg cggcgaagag agccagcgct gcgtgatccg ccatgtcgcc
     1621 tatatggggc cgcagtatga agtgacggtg gaatggcacg ggcaggagat attattgcag
     1681 gtcaacgcta cgcgtctgca accggacgtc ggcgagcagt attatcttga aatccatccg
     1741 tacggcatgt ttgttctggc ggatgcggca taacgttttg tggttttgta ggccggataa
     1801 ggcgcttgtg ccgccatccg gcaatgcctg atgcgctgcg cttatcaggc ctacaatgaa
     1861 cgatgtagac ccgataaacc ctcagcttcg cgcttttaac gcggcgatat ccgcaggcgt
     1921 ggtgcgacag cacccgccaa tcaaccgtgc gccagcggcc tgccactgcg gcagataatc
     1981 cgccagctgc gcgcaatgtt caccgtgatg atgccaggtt ttgctcacgg catcgtaatg
     2041 ctcgcccgag ttcggataca ccaccagcgg cagcaccgtt aaaccgtgta aatgctgcaa
     2101 cgcagcggtg gtgttttcca gcgcaataca gttaatgcct agcgccacca cctgcggata
     2161 acccgccagc aacgcaacca cgtcacgcag cggcgtaccg tcgctcaggt gttcgctgtc
     2221 gcgcagggta aatgagaacc acgcccgcgc acgcggatat gcggtcaaca gctcggccaa
     2281 cgcctcaatc tcggaaaaat tcggcagggt ttcgcaggcc agcagatcgg ccccggcatc
     2341 cagcaaggct tccacgcgcg ggcgatgaaa cgcctgaaat gcctcaacgc tacaatgata
     2401 atcgccacgg tattcagagc catccgccag atacgcgccg taaggcccga cggatcccgc
     2461 caccagaagc gttcccgcct gcgggttctc tgccagatac gcttcacggg ctttacgcgc
     2521 cagctccacg cttttgccaa tcagcgcttt cgactgcgct tcatccagac ctcgcgcggc
     2581 gaagcccgcc ggcgtcgcct gatagctggc agtgatcgcg cattgcgccc ccgcccggta
     2641 gtaatcaaga tgcacttcgc ggataagctc cgggttttct accagcactt tggctgacca
     2701 caggctgtcg gctaagttac accctcgcgc ttccagctcc gtcgccatcg cgccatccag
     2761 cagcaggata tcctgtttat caagaagagc gcgtaacgga ttattctgcg acatgttctg
     2821 gctcctgttt tgcgtttcgg ggttgagtaa ggaaataagc accatagcac aacgcaacaa
     2881 acggtaaccc gcaccacaac gcaattctct gcgctggatc gaatgccagc ccaacacagg
     2941 ccaccaggca cagcacaaaa cctaatactg gcaccagcgg ataccacggc gcgcgataat
     3001 gtaattcact caatgcctta ccttgttgca gatgacggcg acgaaaaaca aaatgcgagg
     3061 cgcagatact cagccacacc gctaccaccg caaacccgga gattgccgac agcgcaacaa
     3121 ataccgtgtc cggggccacc acgctggaaa acagcgccag cacaccaccg agcatactga
     3181 ccgacagcgc cgtcagtggc acgccgtttt tcgttactcg cgcaaaacag gccggtagcg
     3241 tacgttcatt cgacaacgac cacagcatgc gcccggaggc atataacccg gagttcgctg
     3301 cagaaagaat agccgtcagg atcacgaagt taaaaatatc agcggcgtac gggatcccta
     3361 ctttctcaaa taccagcaca aacgggcttt tctccacgcc cgcctgctgc atcgggatca
     3421 gcgctgccag cacaaacacg gtgccgataa agaaaataat cagtcgcgcg atggtagtac
     3481 gaatcgctac cgggataact ttgcgcgggt tttccgtttc accggcggca atgccgataa
     3541 gctcggtacc cgaaaaagca aagttcactg ccaccatagt catcaaaatc ggtaagccac
     3601 cgtgcgggaa ccagccttct gccgtgatat tactcagccc cggcgcgggc gagccatcct
     3661 gcatcggaat aaagccgaaa atcgccgccc caccgaggat gataaaggcg atgatagtga
     3721 ccactttgac cagcgagaac cagaactccc cttcggcaaa aaagcgcgtg gagataacat
     3781 tcagaccaaa aataatcgcg cagaacacca cgcaccagac ccataccggc acctgtggaa
     3841 accagtactg catacagaat ccagcggcgg taaagctcga acccagcgcc acggtccagg
     3901 tcagccagta aagccaggcc acggtatacc cggtagccgg accaagatag cgcgcggcat
     3961 aaacgtgaaa cgctccggtc tccggcatcg cgaccgacag ctcgcccaga cactgcataa
     4021 ccagccagac caccagcgca ccaatcagat aggccagcag cgttcccgcc gctccagtgg
     4081 tggaaatgat gtacccggta ttgaagaata atcctgtgcc aatcacgccg cccaaggaaa
     4141 gcataatcag gtgacgcgtt ttcattgtgc gcttcagtgg cgcattttgt tgtgttgttt
     4201 gcatataacc ttctaaacgt atggacatct aaacatccaa agaaggtttg ttataccgcg
     4261 tttgttaaca gaatgcaaag tcaaccctgc accagacgca gatgcccgcg ccgggcgctt
     4321 aatagcgggt ggggattaat aatcagatac aaaaagggcc gccagctcat aacgcctccg
     4381 gaaggtgcga acaagtccct gatatgagat catgtttgtc atctggagcc atagaacagg
     4441 gttcatcatg agtcatcaac ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca
     4501 gaccagaaaa gagattttct tgtcccgcat ggagcagatt ctgccatggc aaaacatggt
     4561 gga
//
LOCUS       DAPPNJ010000270         4528 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000270 DAPPNJ010000000
VERSION     DAPPNJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.272"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(18..476)
                     /locus_tag="RPM73_004188"
     CDS             complement(18..476)
                     /locus_tag="RPM73_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDX6522461.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            596..1405
                     /gene="proC"
                     /locus_tag="RPM73_004189"
     CDS             596..1405
                     /gene="proC"
                     /locus_tag="RPM73_004189"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDX6522462.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQFADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1422..2537)
                     /gene="adrA"
                     /locus_tag="RPM73_004190"
                     /gene_synonym="dgcC"
     CDS             complement(1422..2537)
                     /gene="adrA"
                     /locus_tag="RPM73_004190"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDX6522463.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2639..2959)
                     /gene="psiF"
                     /locus_tag="RPM73_004191"
     CDS             complement(2639..2959)
                     /gene="psiF"
                     /locus_tag="RPM73_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDX6522464.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3078..4493)
                     /gene="phoA"
                     /locus_tag="RPM73_004192"
     CDS             complement(3078..4493)
                     /gene="phoA"
                     /locus_tag="RPM73_004192"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDX6522465.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
BASE COUNT         1040 a         1246 c         1139 g         1103 t
ORIGIN      
        1 taaataataa attacattta gccacgacta cgttgcactt ccagccacca cttctccagc
       61 tccgcggcaa aggcctggcg gtcacgttgt gaaaggctat ctggtccgcc ggtctggatc
      121 ccactggcac gtaaggtatc cataaaatcg cgcatcgtca ggcgctcacg aatggtcgct
      181 ggcgtgtaac gttcgccgcg cggattaagc gccgctgcgc ctttctcgat ggcttcagca
      241 gccaaaggta tatctgcggt gatcaccaaa tcgcccgctt cacactgccg gacaatttcg
      301 ttatcggcaa cgtcgaaacc tgccgcgacg cgcagcgtac gaataaatcg cgatggcggc
      361 acgcgtaaac tctggtttgc taccagtacc agcggcatct gcatacgttc cgccgcgcga
      421 tacaaaatct ctttaattac attgggacac gcgtcggcat ccacccaaat tgtcataaag
      481 tcatcctttg ttgggtaatc ctctattgtg tcgcgctttt gccttccggc atagttctgt
      541 ttatgcttct gccagcgatt atcaaaacaa tgaatttcac ggcaggagtg aggcaatgga
      601 aaagaaaatc ggttttattg gctgcggcaa tatgggaaaa gccattctcg gcggtctgat
      661 tgccagcggt caggtgcttc cagggcaaat ctgggtatac accccctccc cggataaagt
      721 cgccgccctg catgaccagt tcggcatcaa cgccgcagaa tcggcgcaag aagtggcgca
      781 attcgccgac atcatttttg ctgccgttaa acctggcatc atgattaaag tgcttagcga
      841 aatcacctcc agcctgaata aagactctct ggtcgtttct attgctgcag gtgtcacgct
      901 cgaccagctt gcccgcgcgc tgggccatga ccggaaaatt atccgcgcca tgccgaacac
      961 tcccgcgctg gttaatgccg ggatgacctc cgtaacgcca aacgcgctgg taaccccaga
     1021 agataccgct gatgtgctga atattttccg ctgctttggc gaagcggaag taattgctga
     1081 gccgatgatc cacccggtgg tcggtgtgag cggttcttcg ccagcctacg tatttatgtt
     1141 tatcgaagcg atggccgacg ccgccgtgct gggcgggatg ccacgcgccc aggcgtataa
     1201 atttgccgct caggcggtaa tgggttccgc aaaaatggtg ctggaaacgg gagaacatcc
     1261 gggggcactg aaagatatgg tctgctcacc gggaggcacc accattgaag cggtacgcgt
     1321 actggaagag aaaggcttcc gtgccgcagt gatcgaagcg atgacgaagt gtatggaaaa
     1381 atcagaaaaa ctcagcaaat cctgatgact ttcgccggac gtcaggccgc cacttcggtg
     1441 cggttacgtc cggctttctt tgctttgtaa agcgccaaat ctgccgattt caaccactca
     1501 cgatagtgac tcatttgtgg gttcagcggc gcaaccccca cactaatccg taaagttacc
     1561 tgtggcgtat tcggcaaacg taatgtattt agcccttcat gcacccgtaa catggcggta
     1621 atggcgctct cagctggcgt accggacatg attactgcaa actcatcgcc gccaaaccga
     1681 ccaatcacat cgctaccgcg cagggtaatt tgtaactgtc gggtaagcgc cacaatcgct
     1741 tcatcgccca catcatggcc ccaggtatcg ttgatgctct tgaaatggtc gatatcgata
     1801 atcagtaacg ttgcatcgcg attatgccgc cgacagttat caaattcatt gcgtaacata
     1861 gtttcccaat gacgtcggtt atacacgccc gtcatgccgt cgcgggtact catgacctgc
     1921 aacctgcgtt tatgttccgc cagtttggtt gccgtctggt agctgaccca gccaaacagc
     1981 agaggataaa tgacaataat gggaagggag agccaccatt ccagcggcgc actattgaac
     2041 gacacggtga tgcccgtcag ctcgagggtg acaaggcagg aaaccaccat caacaccaga
     2101 cccgcgacaa acagacgggg gccgcctgcc cccatcaaat tcagacacat aatcatcaac
     2161 atagcggtgg aaggcagcac gtttacgccc attacgccta cccacattcc cgctaatact
     2221 gcatcggttt ttaagttgta aatttcccgg ctaagcggat cgacggccct gctcgctatc
     2281 tgccaggcta aatgcggcca gacgaacgcc cagccgacca acaccagcca ccaccagccc
     2341 ggcggcgggt gcgaaaccag cgttgaagca atcggtaaga acatgccagc caggccaacc
     2401 gcacggggta gtctgacgcg acgggcgaag cgcagcccgg accgctgatg ttcgttttga
     2461 ggagttaaag gaggttcctc cccatgcgcc gccgcttttt tgaaaaagtt ttcatcattc
     2521 attatttttg ggaacattca gaaacaattt tcccaaatta tagagacggg gctaaaggca
     2581 gaagtatgaa atttcgggtg agccactttc gtagctcacc cgttagcact atcaggtatc
     2641 aggcggcttt cttgaggcag gcactcataa acttattacg atcatcacct ttcagagatt
     2701 gttgcgttgc ttgattatta cattcgcgca tcttttgctg ctgtggcgtc aaactttttt
     2761 caccaggcgc agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc
     2821 ctttcaacgc ctgcgccgtc gcctgctgat tacaggaggt catacgctgt tgttgtgggg
     2881 ttaaggttct ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa
     2941 ccagtaatgt tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtttgcgc
     3001 ccgtgatctg ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt
     3061 cactgccggg cgcggtttta tttcagcccc agagcggctt tcatggtgta gaagagatcg
     3121 gtctggtcgg tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac
     3181 tgactgccgg tatgttcttg tgaatcctct tcggaattcc cgtaactcat caccatcact
     3241 gcgccatctt tagtatttag cgcctgagtg aggcccggcg ctttggtatc cggcgcaaca
     3301 acctggctgg cgtgggcgtg atcagcggtg actatgacca gcgtgttacc ctccttttta
     3361 gcgaattcca gcgcccgttg tacggcttca tcgagatcga ccgtctcgcc aatttgccca
     3421 caaggattcg cagcgtgatc ctgtttatcg attgacgcac cttcaacttg caggaaaaat
     3481 cctttctcat ttttactcaa caattcaatg gctttgtcgg tcatctgcgc cagggttggt
     3541 acactgtcat tacgttgcgg attaggcgta caggtgactg cgggcttatc gatattgccg
     3601 tggtacgttg ctttcggtcc tagccagcgc actggcatat tgccgtcagc aaacaggcca
     3661 agcaggggtt tttgctgatt cgcttctgtc accgcattta gcgaggcggc atcgttcacc
     3721 aactgataac cacgcgcctg tgcctgttca cgcagcgttt ttccctgcca ttcaccggcg
     3781 gttgccgttt cagcaaaggt ttttgcgcca ccgccaagcg taacgtcggc acgagcgtta
     3841 agcagctgtt cggtaatcga tccttttccg cctttttcca gagcgttacc cggacatttt
     3901 tcactggtcg cgctcggacc gtagcatttg cgcgaagtca catgtgccac cagcgcagcg
     3961 ggcgtggcat cctgcaactc tgcggtagaa acgttaccgg tcgccagacc tgcggctttt
     4021 gccatttcca gaatcgttgg gtgatctttt tcgtgaatat cgacgcccag cgcgccgtta
     4081 taggttttga caccggttga ccaggcggtt gctgatgcag ccgagtcggt gacgtagtcc
     4141 ggtttgccag tttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca
     4201 tcgatacctt taaaaaagcc gcccgcacct tcggcataat tacgtgcggc agtaatttcc
     4261 gaatccccca tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga
     4321 gaatcacgca gagcggcggt ctgatcaccc gttaagcggc gagcaccgcc gggtgcagta
     4381 atatcgccct gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc
     4441 acaggggtaa acagtaacgg taagagtgcc agtgcaatag tgctttgttt cactttattt
     4501 tctccatgta caaatacatt aaaaaata
//
LOCUS       DAPPNJ010000271         4510 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000271 DAPPNJ010000000
VERSION     DAPPNJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.273"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..264)
                     /gene="chbB"
                     /locus_tag="RPM73_004193"
     CDS             complement(<1..264)
                     /gene="chbB"
                     /locus_tag="RPM73_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDX6522466.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVD"
     gene            complement(563..901)
                     /gene="osmE"
                     /locus_tag="RPM73_004194"
     CDS             complement(563..901)
                     /gene="osmE"
                     /locus_tag="RPM73_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDX6522467.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1103..1930
                     /gene="nadE"
                     /locus_tag="RPM73_004195"
     CDS             1103..1930
                     /gene="nadE"
                     /locus_tag="RPM73_004195"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDX6522468.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2160..3047
                     /gene="cho"
                     /locus_tag="RPM73_004196"
     CDS             2160..3047
                     /gene="cho"
                     /locus_tag="RPM73_004196"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDX6522469.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3007..3582)
                     /gene="ves"
                     /locus_tag="RPM73_004197"
     CDS             complement(3007..3582)
                     /gene="ves"
                     /locus_tag="RPM73_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDX6522470.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSCGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3785..4270)
                     /gene="spy"
                     /locus_tag="RPM73_004198"
     CDS             complement(3785..4270)
                     /gene="spy"
                     /locus_tag="RPM73_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDX6522471.1"
                     /translation="MRKLTALFVASTLALGAANLTHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1129 a         1087 c         1090 g         1204 t
ORIGIN      
        1 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
       61 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      121 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      181 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      241 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      301 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      361 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      421 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
      481 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
      541 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
      601 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
      661 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
      721 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
      781 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagccgtac
      841 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
      901 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
      961 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     1021 attcaacccg ctgtcgcttg tcgtttctgt agcaacgggt tagctttaag gaagttttgt
     1081 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     1141 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt atcgattttc tgaaaagcta
     1201 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     1261 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgcctgg aaaccggcaa
     1321 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggccg acgaacaaga
     1381 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     1441 cgcggtattg gccagcgaac aggcattgcg ggaagcaggc attgaactga gcgattttgt
     1501 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     1561 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     1621 atatggtgac ggcggtacgg acattaatcc gctgtatcgt ctcaacaaac gtcagggtaa
     1681 acagttactg gcggcattag gttgcccgga acacctttat aagaaagcgc caacggccga
     1741 tctggaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     1801 tatcgacgac tatctggaag ggaaaaacgt acctgaacag gtcgccagaa caatagagaa
     1861 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt accgttttcg atgatttctg
     1921 gaaaaagtaa taatttgcac ctattggttt gtgcgaaaaa gagtaatttg ttcacgccgg
     1981 atgcggcgtg aacgccttat tcgacctaca aaactatgca aattcaatac attgcaggag
     2041 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     2101 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     2161 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     2221 ccgaacattt acgttcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     2281 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     2341 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     2401 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     2461 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     2521 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     2581 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     2641 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     2701 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     2761 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc
     2821 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     2881 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     2941 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     3001 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     3061 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc
     3121 ctggtcggta gtgagcaatt tatcgcccag ttgccacgcc ccgttaatca caaacaccac
     3181 cccgccgcat gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     3241 gacatccagg cgtgtcatga tgttgaaatc catcgacatc tgccctgccg tcagtttcgc
     3301 ttttaccacc tggtccgctg caaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     3361 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     3421 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     3481 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcatttcg
     3541 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     3601 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     3661 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     3721 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     3781 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     3841 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     3901 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     3961 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     4021 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgcggatct
     4081 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     4141 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     4201 cggcatgggt caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     4261 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     4321 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     4381 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     4441 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     4501 ggatgactgg
//
LOCUS       DAPPNJ010000272         4473 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000272 DAPPNJ010000000
VERSION     DAPPNJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.274"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..564
                     /locus_tag="RPM73_004199"
     CDS             <1..564
                     /locus_tag="RPM73_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDX6522472.1"
                     /translation="IVYPPILSNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTT
                     PSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNF
                     QSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEI
                     HLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(572..2053)
                     /gene="clsC"
                     /locus_tag="RPM73_004200"
     CDS             complement(572..2053)
                     /gene="clsC"
                     /locus_tag="RPM73_004200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDX6522473.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="RPM73_004201"
     CDS             complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="RPM73_004201"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDX6522474.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2623..2934)
                     /locus_tag="RPM73_004202"
     CDS             complement(2623..2934)
                     /locus_tag="RPM73_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX6522475.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="RPM73_004203"
     CDS             complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="RPM73_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDX6522476.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="RPM73_004204"
     CDS             complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="RPM73_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDX6522477.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3942..4397)
                     /gene="csgB"
                     /locus_tag="RPM73_004205"
     CDS             complement(3942..4397)
                     /gene="csgB"
                     /locus_tag="RPM73_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDX6522478.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1120 a         1127 c         1000 g         1226 t
ORIGIN      
        1 attgtgtatc cgcccattct gagtaatggc atgttcaatt ttattgtcat gcaaacgctg
       61 ttttatttgc cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc
      121 ttgtttacca cgccgtctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat
      181 ttactcaacc agcgctatgg cagtggcgat gcctggatgt acgaaaccga gtcggtgatc
      241 accatggtcc tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt
      301 aacttccagt cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt
      361 caccacccgt taacgctgtt tttcggcgca tacattacac cgcacatcac ctccaactgg
      421 cttggttttc tctgtggcct gatatttgta gtagggattg cgataattct gtatgaaatt
      481 catttgcgca tcccgttact gaagtttttg ttctctggta aaccggttgt taagcgtgag
      541 aacgataaag caccagcccg ttaagccaca tttacaataa ccattccacg ggcaatatcg
      601 acgccagtct gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa
      661 tctctttctt tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct
      721 gccaggccgc atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt
      781 cgctctctat cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga
      841 aagagccgat aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggctc
      901 cggaattacc ggttatgccg cgatcgtgta acgtactact ttgttcacgc gtcggcttga
      961 gttcatataa ttccacgcca tagcggagca attttttgcg ccagcgcgcg tatccggcat
     1021 ggacgacagc aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt
     1081 ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga
     1141 taatatcgat gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg
     1201 aatgccgttt tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgttttcg
     1261 cccagataag cggcaacgtt ccatcaacca gatgatttat aaacggactg gattccattt
     1321 tgcgtaagta acgatgcgtc atagcatcgt tatgccagga ggcgggtaac tcgatgcgat
     1381 ccgccatttc accttccggg acatccagca cttgctgtaa gggtgaaacc gatttgcaat
     1441 accagtagcg ggcgaaatca tcggcaacgt cctctaccac gggccctatt gccataacat
     1501 ctaaatccga aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgcc
     1561 cccctaccag cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga
     1621 gacgggaaaa gtcggtgata taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa
     1681 aaagccggac ttcaatgcgg ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg
     1741 gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca
     1801 gggcggaaaa cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca
     1861 gcgtatgctc tgccatttcg gccagacgat accgggcggc aaacgcatcc agacttttct
     1921 ccagagggaa aagaccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg
     1981 ccagccgggg caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg
     2041 ttttcttcat cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat
     2101 tctgaaacgg ttttaactgc gatttcagcc gctgccgcac gagggtaacc ataaacccca
     2161 gtactgattg caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgtaggcta
     2221 ttgagatagg catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc
     2281 ccgacggtgt gcactacggc tttagcggga agagcgcctg caagcgtaat aacggcatgc
     2341 cccgtagggc aatcgccctg ctgttgcctg acttttaaac aggcatccag cagggccgga
     2401 cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc
     2461 gcattcacaa tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc
     2521 gttttcataa cgcctcctta caccttcttg tttcttaagt gtacagcagg ctttttggtt
     2581 aaatcgcatt tttctgctca gcaacgtgcg ttgttcagaa acttagttat attcgatgtt
     2641 cattacagcc agctttttct gtttatcaag atactgcact tttactgagg ctatttgcgc
     2701 gtttttaaca ttaccgctca ttaacgcttt gctggaatag gttttgcccg gaacagaacc
     2761 attctgtggg cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc
     2821 cacaatttgg ccataaaaat ggatcacccc cccttgcgcg gtgcttcctg cgatggcaat
     2881 aaaaggaaaa agcaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc
     2941 tatttcctca atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg
     3001 attcgcagca gaccattctc tccagattca tcttatgctc gatatttcaa caaattaaga
     3061 cttttctgaa gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt
     3121 aacaactatt ttcacccgat cttccgggga aatatttaaa ctcaacttcg tcaaagcaat
     3181 aggttgatta gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact
     3241 gccttcgcgc agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc
     3301 aggaataatg gtatacacat ccccttgctg ggtcgtatta aaggttatct ggctggaaag
     3361 tgccgcaagg agtaataacg cattcatatt cttctcccga aaaaaaacag ggcttgcgcc
     3421 ctgtttctgt aatacaaatg atgtattagt actgatgagc ggtcgcgttg ttaccaaagc
     3481 caacctgagt cacgttgacg gaggagttag atgcagtctg gtcaactgca gcaccgttgc
     3541 caccaccgaa ctgtttaaca gtcatttcag aatttttgcc gttccactga tcaagagtgg
     3601 cgctgttacc gaagccacgt tgggtcagat cgattgagct gtcatctgag ccctgaccta
     3661 catctgcacc attaccaccg ccatgctggg taatagtcaa gtcagagtta cgggcatcag
     3721 tttgcagagc aagtgcagag ttaccgccac cgtactggta aatgttcagc tctgaatttg
     3781 ggccgctatt attaccgcca ccaccgtggt taccgccgcc gccgtactga ggaacaacac
     3841 ctgccagagc gctaccggag aatacgattg ctgcaattgc tgctactttt aaaagtttca
     3901 tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa attaacgttg tgtcacgcga
     3961 atagccattt gcgactgtct ctgcactaca attgccgttt tttgagtacc atactgtgta
     4021 atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct
     4081 tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct
     4141 cctgtctggt caatctttgc ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt
     4201 tttgagcctc cctgccgtaa ctgagcacta ttattcgtcc cagcttgacc aattatggct
     4261 gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc tgaattagct
     4321 aaatcataac ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat
     4381 aacaatttgt ttttcatgtt gtcaccctgg acctggtcgt acatttaaga aattaaatca
     4441 tttcaacttg gttgttaacg caacctgtat ttt
//
LOCUS       DAPPNJ010000273         4439 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000273 DAPPNJ010000000
VERSION     DAPPNJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.275"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            170..1327
                     /gene="acrE"
                     /locus_tag="RPM73_004206"
     CDS             170..1327
                     /gene="acrE"
                     /locus_tag="RPM73_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDX6522479.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1339..>4439
                     /locus_tag="RPM73_004207"
     CDS             1339..>4439
                     /locus_tag="RPM73_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDX6522480.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1059 a         1085 c         1186 g         1109 t
ORIGIN      
        1 atattaatct ttttttgtgt ttatgtgcct tgagacgcct gtattcataa ctattcctta
       61 catcgacgaa tgataatttg taggatagcg aactgtattt ttctttctgc gagttaacgc
      121 gttgcctttt tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      181 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      241 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      301 aaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      361 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgcaatttca ctgaaggcag
      421 cgatgtgcaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      481 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      541 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      601 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      661 aagcgctcgc atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      721 caaatcgacc gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      781 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      841 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      901 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt
      961 gaccgttgat gaaagcactg gctccataac cctacgtgcc gtcttcccta acccgcaaca
     1021 tacgcttttg ccgggtatgt ttgtgcgcgc acggattgat gaaggcgtcc aacctgacgc
     1081 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1141 tgttaacgat aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1201 taaatggttg attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1261 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1321 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1381 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1441 tatccaacca ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg
     1501 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac
     1561 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1621 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1681 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1741 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1801 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1861 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1921 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     1981 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt
     2041 attgctcaaa cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2101 agtgacggct cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2161 tataacgtta tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca
     2221 accggcgcga atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2281 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag
     2341 ctttctattc acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg
     2401 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2461 gtgttgttag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2521 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2581 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca
     2641 atgtcgcaaa tccagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt
     2701 ccgatggcat tcttcggcgg ttctactggg gcaatttatc gccagttctc tatcaccatc
     2761 gtttcggcaa tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca
     2821 acgctgctta aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg
     2881 tttaatacta ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc
     2941 ggttccacag gacgatattt actgatctat gcgctgattg ttgcaggaat ggtggtgttg
     3001 tttttacgtc ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgaccatg
     3061 attcagttac ccgctggcgc gacgcaagag cggacgcaaa aagtgttgga tcaagtgacg
     3121 gattactatc tgaagaacga gaaagcgaac gttgaaagtg tctttacggt taacggcttt
     3181 agcttcagcg gccaggcaca aaacgccggt atggccttcg tcagtctgaa accgtgggaa
     3241 gagcgtaatg gtgatgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg
     3301 ggcaagatcc gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc
     3361 acggcaacgg gtttcgactt tgagttaatt gatcaggctg ggctgggtca cgatgccctg
     3421 acccaggccc gtaaccagtt gcttggcatg gcggcgcaac atcctgccag cttagtcagc
     3481 gtgcgtccta atggcctgga agacaccgcg cagtttaaac tggaagttga ccaggaaaag
     3541 gcgcaggcat taggtgtttc actttctgac atcaatcaga ccatttcaac ggcgttgggt
     3601 gggacttacg ttaacgactt catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg
     3661 gatgccaaat tccgtatgct gccagaagat gtcgataaac tttatgtccg cagcgccaac
     3721 ggcgaaatgg tgccattctc ggcctttacc acttcacatt gggtgtatgg ctctccgcga
     3781 ctggaacgct acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc
     3841 agttccggcg atgccatggc gttgatggaa aaccttgcgt caaaattacc tgcgggcatt
     3901 ggttatgact ggacgggtat gtcgtatcag gaacgcttat cgggaaacca ggctcccgct
     3961 ctggtagcaa tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg
     4021 tcaattcctg tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctgctggcg
     4081 gcgacactct ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt
     4141 ggcttgtcgg ccaaaaacgc tattttgatt gttgagttcg ctaaagatct catggagaaa
     4201 gagggtaaag gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc
     4261 ctgatgacct ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgcc
     4321 ggcagtggcg cgcagaacgc agtgggtatc ggggtaatgg gcggaatggt ctctgcaacg
     4381 ttgctggcaa tcttcttcgt accggtgttc tttgtggtga tccgccgttg ctttaaagg
//
LOCUS       DAPPNJ010000274         4399 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000274 DAPPNJ010000000
VERSION     DAPPNJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.276"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            57..899
                     /locus_tag="RPM73_004208"
     CDS             57..899
                     /locus_tag="RPM73_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDX6522481.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPESSPAEALIITNQIQEIVPEEISSTTPPVPVRKKHKGLMGLALTAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENSSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(958..1935)
                     /locus_tag="RPM73_004209"
     CDS             complement(958..1935)
                     /locus_tag="RPM73_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HDX6522482.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDMANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQVERLAQSLQQGGAGISQMLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(1952..2881)
                     /gene="flgL"
                     /locus_tag="RPM73_004210"
     CDS             complement(1952..2881)
                     /gene="flgL"
                     /locus_tag="RPM73_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDX6522483.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQDPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(2896..4272)
                     /gene="flgK"
                     /locus_tag="RPM73_004211"
     CDS             complement(2896..4272)
                     /gene="flgK"
                     /locus_tag="RPM73_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDX6522484.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYVDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLQNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
BASE COUNT          930 a         1195 c         1218 g         1056 t
ORIGIN      
        1 gctttatgca aagcactatc aggtaacatt tatcccttgc taaggtaaac tcaactatga
       61 gtattattat caacaactgg cggatggacc cgtcgcttaa tgccttaata cattgcgaaa
      121 cgggtgaaac acgtcgtctc ggcgaatacc attttatttt gctggaaaca ttagcaaaaa
      181 atgccgatgt ggttttatcg cgatcttatt tatgtgccga agtatggaag aaccgcattg
      241 tcggcggtaa cagcctgccg acggcaatcc atgcgttgcg cgttgctatc gacgatgatg
      301 gcaaacaaca gaacattatt aaaaccatcc cgaaaaaagg ttacctgtgc aataaagagt
      361 atgtgtcttt gccggagtca tcgccagcgg aagcgctgat tattaccaac cagatacagg
      421 aaattgtgcc agaagagata tcttcgacaa caccgcctgt gcctgttaga aaaaaacata
      481 aaggactgat ggggttagcc ttgaccgcgg ctgttatatt tattggctcg accgtggggt
      541 attcacactt gaaaagtaca cctgatgccc cgcaactggt aaaagagtca attaacagtc
      601 caagaataaa aatatttcac cttagttctg gaaaagaaaa tagttccgta ccgttactgt
      661 cacaaaccct cgcaccaggg aaagacaaac tcgataattt attgtcagcg cataatatga
      721 caatgacaac ctattataaa tatgttcgta accgactgga aagtgatatt gttctgcgca
      781 accagtgcaa tggtagctgg caattaactt ttaatgtgga aggctggcag aacagcgata
      841 ttaatagcgc gatgtatcag aatttagaga agttgttaaa tactgtgcaa aaatgctgat
      901 gagtgccaat atatgccgga tgcgacgcta aacgcgtctt atccggcctg ccgataatta
      961 ccccaataaa ttcctcaccg tctggctgga aattcgcgcc tgaccgttca ccgcctgcaa
     1021 taacacctga tagttatgat tggcgctatc gagcacgccg ccgaggtttt ccgacgcctg
     1081 caccgcgttt tccgtttgcg ggaagcgcga catgccgtcg atcaactgcc gcgccttttc
     1141 ctgctgcacc gccatctgcg cgcgctgctg attaatgctt tccagcattt gagaaatccc
     1201 cgccccgccc tgttgcaggc tttgcgctaa gcgctccacc tgtgatggtt cggcaaaggt
     1261 ttttagcggc ataaacgccg acttatcgcc atcggtgcgc acgcttaagg tgcgctcgat
     1321 atcaggccac tgtttttcag tggtggagaa actgatgcca tccgcttgct gatgcaactg
     1381 aacgcccacg cggcgcaggg cgttggtcag ccgcgtctga tactgcccgg cgctgtcatc
     1441 ctcagacagc atcaccgccg aaagctgagt ctgccgcccg tcagagacgc tgaacatccg
     1501 cgttcccggc gtgggattgt gtaccagatt cgccatatcc ggcgagtgaa aagtcacccg
     1561 cgcttcaccc tgcaacactg gctgcaactg gcggtccacc gcgccgccgg aaagcgcggt
     1621 gcgtttgtcc agcatctgca tcagcgccgt actctgcgcc tgcccgccct tacgctggct
     1681 atgacgataa tcgagcaact gctgctccag ttgcccgaga taatgatccg cctgctgcaa
     1741 cgtggtgagc tggtcgttaa gctgcacgct gtaacgctgc gggcgagtgg taatcagcgg
     1801 cgaggccgga tattcactcg ccgtcgccgg gagtttttgc cgcaccgcct gagtgggcgc
     1861 aacggttgac gacgataccg cagcgctatg cgcgccgccg gtggcgagtg atgaagatgt
     1921 aagtccgacc tgcatacggt tcctgaaaga gttagagaac gctgaataaa ttcaactcac
     1981 tcactttcga gtaagtcttg agggtagctt ccattgccac ttccagtccg gtaaaggtga
     2041 tcgacgccgg gccgtaatcg agatcctgca atgaaccaat caactcatca ttagaggtag
     2101 agatatcggt ctgcgcatcg ctcagcatcg acatggtgtt ctgcgtctcg ccgagcgacg
     2161 caatcgaggc gttgaggtcg tctgaggcgg tgtccaccac atcaacggca ttctgaatat
     2221 cgctctgcac ctgcggatct gccgggtcaa cgtccgggtc ttgcaactcc tgagagagcg
     2281 aattgagcgt gttgagcaca tctaaattgc taccgaagaa atcgctcgcc gccacgttgg
     2341 tatccacttc cacgccgttc gaaacggtgg tctggcggtg gtcgctgttg ccctggtagc
     2401 tatagctgtc ggtcacgcca tcgccgtcat cgtccacggc cacaatcggc ggctgatcgt
     2461 tgatggtgcc gccaaacacg tagtgcccgt cttcgtcctg ataattgagc gccgcgacca
     2521 tcgattcggt gagcgactca atatcctgcc cgaggcttgc gagcgaatcg gcggtattgg
     2581 tgccgttcgc cgcttccagc aggtcgtcac gcaccgccag caggctgttg ttgacgccat
     2641 cgagaatcga ctcctgctgg cttaagcccg ccgacgcgga gtcgatattg ctctgatact
     2701 gctcaatcgc cgactgctgg cggtttaact gggtgattcg cgtggcggcg atcgggtcat
     2761 ctgacggctg caaaatctgc ttgccggttg ccatctgctc gaccacatgc gccagatcgc
     2821 cggagagatt attaaaactt tgcgtcatcg agacgtaggt ttgttgggtg gttactcgca
     2881 ttatcgcgct cccgcttagc tgcacatttc cagcaccgaa tcgaaaatct cggccccggc
     2941 ggaaatgact ttcagattgg cttcataaat ttgttgatag gtgatgaggt tcaccgcctc
     3001 ttcgtccatg ctgacaccgc tgacgctgct ctgctggttt tgcgctgcgg aatagacatt
     3061 ggacgcggca tcgacttccg tctggttttg ctgactgtaa atgccgatat tgctgatgat
     3121 tgacgagcac gcctgcccga ccgtcacgct gccgaggttg gcgatctcca gcggttcggt
     3181 ggagatgttg atcagcgcct gcaggttgtc gctgttgccg ctttcgtccg gcgaactgga
     3241 gaacgccagc tcatcggcgg tgatatccgg gttgacggtc agcgggccat cggcattgct
     3301 ggcgtcatag ataaacagcg gctcgccggg gttaccgttg agatcgtagc cctgcgcaag
     3361 ctggttgttg accgcatcgg caaactgcga cgccatgctg ttgatggtgt cggtcaacgg
     3421 cgtcagcaca tcgttttgat aatcaaacag tgcgcctaat gaaccgccgg tgtcggtggt
     3481 catcgtcgag gtggtgccag cgaacgtcag cgacatggtc ggcgtaccgt cggcgttggt
     3541 ttccagcgcg atggtcgagc tttgctgccc gctcaccagc ggctgaccgt tttgcagggt
     3601 gacgttgtag ttgccctggt cgtcgatatt gacctgcaca tccatcatcc cgctgagttc
     3661 ttccaccatc tggtcgcggg cgtcgtacag cgcagaggcg ttatcaccgt tggcttcggc
     3721 ctgcgcgatt tgctggttat agctggcgat gccgctggtg agcgtattga tttgcgacac
     3781 catcgcttgc tgctggctga tgatttccgt gctttgcgag tcgacgtaat cgagcgtgtt
     3841 atcaatacgc aacgacagcg ccccggcttc gctgatcacc tgctcgcgca gggcggaatc
     3901 atcggggctg gtggtcgctt cgttaagggc ggcgaagaag ttatcgaagc cgccgctcag
     3961 gctgctgtta tcgtcgctca gtaccgcttc cagttgcgtg agatacccct gctgggtgct
     4021 gtaatagccg taatcgctgg cggcatacca cacctgattc acctgatact ggttggagac
     4081 gcggcgaatg ctgtcgacct gtacgccgtt accggcgctg ttcggcgaac cgccgctggc
     4141 accgatggtg ctgatctccg ccacctgacg ggtgtagccc gtggtcatgg cgttggcggt
     4201 gttttgcgcc gtgacgttaa gctccacctg cgcggttgat gcaccgctgt agccgatgtt
     4261 aatcatgtcc atgttggatc cttaccggta aatcgtgccg gttaaaagcg gcgtaaagtt
     4321 ggggattctt aagttccgta aaccgggtgc cgaacgccgg atgcggcgtg gacgccttat
     4381 ccggcctaca tacgggcga
//
LOCUS       DAPPNJ010000275         4395 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000275 DAPPNJ010000000
VERSION     DAPPNJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.277"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            135..707
                     /locus_tag="RPM73_004212"
     CDS             135..707
                     /locus_tag="RPM73_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDX6522485.1"
                     /translation="MASATSENTSSTKNEAQTVLSERSLKAEQRGMEIMDDMQYARLA
                     LFSGDTGAADKLINRAEDLLKSDDKTWETVVRKDKKTPQEGDVYIIINSSLMLSEDFT
                     VTPEKQKAIDEANKKLDKGDKKGAIDVLHLAGIDISETQWLMPLKQTQKKVNSAQKLM
                     SEGKYYEANLALKGAEDGVITDTVSLNEGD"
     gene            863..1597
                     /locus_tag="RPM73_004213"
     CDS             863..1597
                     /locus_tag="RPM73_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDX6522486.1"
                     /translation="MFPYCFAIIVRPLCASFYNEELDFARNEILFLKRNDIQYITDAC
                     EGCVRFIDIPCHIISDYLYIRLRDADALCITDRKNSFFRKKAYGTFLNSLMSLAEQQK
                     NTNLYESVLFVLLDYLNDKNQLLPVLSGGLNNYSLRVEAIVAAEPAKKWYLSDVASAL
                     CISASQLKRKLHSEGAGFSRIITEVRMKKAITLMKCGNANIFIVARECGYSSLSYFIL
                     SFRKYYSVTPCQWLKQYGIKDTTGDG"
     gene            1840..3069
                     /locus_tag="RPM73_004214"
     CDS             1840..3069
                     /locus_tag="RPM73_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDX6522487.1"
                     /translation="MSYPVQKKIKTGENVLRAAVERINWVFDTFAEICLSFSGGKDST
                     VLFHLVADIARRKKRRFSVLFIDWEAQYLCTVRHVQKMRELYMDVTESFYWVALPLTT
                     VNGVSQFQPEWICWESGVEWVRQPPDYAVTDPNFFPFYQYAMTFEEFIPAFSSWMARS
                     KRGLAVLTGVRADESLNRFMGLASQHKLRYADDKPWTTASQDGFYYTMYPLYDWKTRD
                     IWIYNAKTHAIYNPLYDLMFRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMC
                     ARVSGAASGALYANESGAYFALRKRISKPDHHTWRSYAMFLLNVMPEKTAEHYRNKIA
                     VYLHWYQTHGYPDDIPDEQDNDLGSRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHY
                     ERYLQRMKERRKEWGIL"
     gene            3054..3692
                     /locus_tag="RPM73_004215"
     CDS             3054..3692
                     /locus_tag="RPM73_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDX6522488.1"
                     /translation="MGDPLTPEILTVMIRQYFCQHLSEEETINALNHLRRVLHEISPF
                     AREPVDCVLWVKSDEVIANDYNPNVMAPGEKKLLKQSLEQDGFTQPVVVSEEKDHYLV
                     VDGYHRQLLGRGAGTEKRLRGWLPVTCINPERKGQATRMAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWSDERIGTELGMDQDEVLRLKQISGLTELFQDEDFSPAWTVR"
     gene            3677..4033
                     /locus_tag="RPM73_004216"
     CDS             3677..4033
                     /locus_tag="RPM73_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDX6522489.1"
                     /translation="MDGSLIQLNKILVDEFLSTQKRALEAVDDLIALKLEAAGCWRRA
                     SARWLVVMGAGDITDAQREWLLRRRAYCMAQTTSHVLHEKMNIRGVAKAADETLKRMG
                     IADLSEEMFRKRPSYY"
BASE COUNT         1244 a          827 c         1097 g         1227 t
ORIGIN      
        1 actaatcgtc agggggaaaa tcatattatg tttattatca ggtatgattc tttctgcctg
       61 atgtcggagt aaaaaatatg aaacatatct ttaatgctgt agcaatcgct ctgtccctga
      121 cctctgctgt cgcaatggcc tcagcaactt cagaaaacac ttcatccaca aaaaatgagg
      181 ctcagacagt tttatcagaa aggagtctga aggcagaaca gcgtggtatg gaaattatgg
      241 atgacatgca gtatgcacgt cttgccttat tcagtggtga cacaggagca gcagataagc
      301 tgataaaccg ggctgaagat ttgcttaaat ctgatgataa aacatgggaa acagttgtcc
      361 gcaaggataa aaaaacacca caggaaggcg atgtctacat tattattaac agcagcttaa
      421 tgttgtctga agactttact gtcaccccgg aaaaacagaa agctattgat gaggccaata
      481 aaaaactgga taaaggtgac aaaaaaggag caattgacgt actgcatctc gccggaattg
      541 atatttcaga aacgcagtgg ctgatgccat taaaacagac gcagaaaaag gttaacagtg
      601 cgcaaaaatt aatgagcgaa gggaaatact acgaagctaa tcttgcactg aaaggtgcag
      661 aagatggtgt gattacggat actgtcagcc tgaatgaagg tgactgataa catttatatg
      721 cgcgtataac cacggtgacc ggactgtatt ttgtccggtt actttaataa taaaaccgaa
      781 cttcttaata taatgtcagg aagtaagtct gtcagctata ataagaaaaa taccggatat
      841 ttctaaataa cacctgtact gtatgtttcc gtattgtttt gccattatag tgaggccact
      901 ctgtgcttca ttttacaatg aagagcttga ttttgcccgg aatgaaattc tctttcttaa
      961 aagaaatgac attcagtata ttacggatgc ctgtgaagga tgtgtgaggt ttattgatat
     1021 accctgtcat atcatcagcg actatctgta tatcagactg agggatgccg atgcgttatg
     1081 catcactgac aggaaaaatt cttttttcag aaagaaagcg tatggtacct ttctgaactc
     1141 gctgatgtca cttgcggaac aacagaaaaa tactaacctt tatgagtcag tattatttgt
     1201 gctgctggat tatcttaatg ataaaaacca gttgctgcct gtattgtccg gtgggctgaa
     1261 taactactcg ttacgtgttg aagctattgt tgccgcagag ccggcaaaga aatggtatct
     1321 gtcagatgtt gcatcagcgt tatgtataag tgccagtcag cttaaaagaa aactgcacag
     1381 tgaaggcgca ggattcagcc ggataatcac agaagtacgt atgaaaaagg caataactct
     1441 gatgaagtgc gggaatgcta acatttttat tgtcgccaga gagtgcggat atagcagtct
     1501 gtcgtatttt attttaagtt tcagaaaata ctactcagtc actccctgtc agtggttaaa
     1561 acagtatggt attaaggaca caactggtga cgggtaaacc ctgattcaca taaaccggtt
     1621 ttctggcgta tacatgctgt acagtcatcc aggccttata aggggtatat tgattgtttt
     1681 gtgatatgtt tcaatttttt gttgttgtta ttgttatatt ttttaaaaaa tcttactcat
     1741 tgtgaaacga tttttatcgt gcactgtgct tgattacatg gttaccagag gtgtctctgt
     1801 gtaagtgatg tttctgtatg tttattacat tgagaacaaa tgagttatcc ggtacagaaa
     1861 aaaataaaaa caggtgaaaa tgttctccgg gctgctgtgg aacggattaa ctgggttttt
     1921 gatacttttg cggagatttg tctgtctttt tccggaggta aggattccac tgttttattt
     1981 catctggttg cggatattgc ccgcagaaaa aaacgacgtt tttccgtgtt gtttatcgac
     2041 tgggaagcgc agtatttatg tactgtccgc catgtacaaa aaatgcgtga attgtatatg
     2101 gatgtcacag agtcttttta ctgggtggcc ctgcctctga caacggtgaa tggggtttcc
     2161 cagttccagc ctgaatggat atgctgggag tctggagtgg aatgggtccg tcagccacct
     2221 gattatgctg ttactgaccc gaactttttc cctttttatc aatatgccat gacatttgaa
     2281 gaattcattc cggctttttc atcctggatg gccagaagta aacgtgggct ggcagtgctg
     2341 accggagttc gtgcagatga gtccctgaac aggtttatgg ggctggcttc gcagcataag
     2401 ctacgctatg cggatgataa accatggacg actgcatcac aggacggttt ttactacacg
     2461 atgtaccctc tgtatgactg gaaaacccgg gatatatgga tatacaacgc gaaaacgcat
     2521 gccatttata accccctgta tgacctgatg ttcagagctg gcattccatt acggaacatg
     2581 cgtgtctgtg aaccctttgg ccctgaacag cgtaagggat tatggcttta ccatgttctg
     2641 gagcctgaaa cctgggccag aatgtgtgcc cgggtctcag gagcagccag tggagcgctg
     2701 tatgcaaatg aaagtggtgc ttattttgcg cttcgaaaac gtatatcaaa gcctgaccat
     2761 catacctggc gcagctatgc gatgttttta ctgaatgtga tgccggaaaa aacggcggaa
     2821 cattaccgga acaaaatagc cgtatatttg cactggtacc agacacatgg atatcccgat
     2881 gatattcctg atgagcagga taatgatctg gggagtcgtg atgttccgtc atggcggcgt
     2941 atatgtaaga cgctcataaa gaatgatttc tggtgccgga ccctgtcctt cagcccaaac
     3001 aaaccccgtc actatgaacg ttatctgcaa agaatgaaag agaggagaaa ggaatggggg
     3061 atcctttaac gccggaaata ctgacagtca tgatccgtca gtatttctgt cagcacctgt
     3121 cagaagaaga gacaattaat gcgctgaatc atctgcgcag ggtgcttcat gagatcagcc
     3181 cttttgcccg ggaaccggtg gactgtgtgt tatgggtgaa atcggatgag gtgatagcca
     3241 acgattataa tcccaatgtc atggcgccgg gtgagaagaa actgctgaag cagtcactgg
     3301 agcaggatgg gtttacacaa ccagtcgtgg tatcggaaga aaaagatcat tatctggtgg
     3361 tggatggtta tcatcgtcag ttgctgggaa gaggggccgg tacagaaaaa cgcctccggg
     3421 gatggcttcc tgttacctgc attaatccgg agagaaaggg gcaggcgacc cgtatggccg
     3481 cgaccatccg tcataaccgg gccagaggaa agcatcagat tacatcaatg tcagatatcg
     3541 ttcgtgacct gtcccgtctg ggatggtcgg atgaaagaat tggtactgag ctgggtatgg
     3601 accaggatga agtgctgagg ctgaaacaaa tcagcggact gactgagctg tttcaggatg
     3661 aagatttcag tccggcatgg acggttcgtt aattcaatta aataaaattc tggtggatga
     3721 gtttttgagt acgcagaaga gagcactgga agcagtggat gatctgatcg cactgaagct
     3781 tgaggctgca ggatgctggc gcagggcttc tgcacgatgg ctggtcgtga tgggggcggg
     3841 cgatattaca gatgcacaac gggagtggct tttacgacga cgggcatatt gcatggcgca
     3901 gaccacttct catgttttac acgaaaagat gaatatccgg ggggtggcaa aagctgcaga
     3961 tgagaccctg aagcgtatgg gaattgcaga tctgtcagaa gagatgttca gaaaaagacc
     4021 atcatactac tgataacggg ctctctgtgg ctggcttctg aagtggaata tatgtgaaag
     4081 agaccggata tattgcgctt tttactttca ataaatcagt aaacaggatt tgtgatgtat
     4141 tatttatcat aatataacaa attgtaatca gtcagagtct gcagactttg tcaggcttca
     4201 gatatcttat tgtggatttc tgtatgtttg tagacgtttt ttcggaaaga catgctgtgg
     4261 acagcaagtg ttgtatgtag ccctcgaatt ggatgcaatc tttgttgctg catttctgat
     4321 gcgcgtttct ggcatatgat ttgccggact gtcccttcga cctgaaaaga tgccagaaac
     4381 gcgactgttt tattc
//
LOCUS       DAPPNJ010000276         4360 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000276 DAPPNJ010000000
VERSION     DAPPNJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.278"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..918
                     /locus_tag="RPM73_004217"
     CDS             <1..918
                     /locus_tag="RPM73_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX6522490.1"
                     /translation="HLTKSAEAVLGMATAPVYIFNSELQNLRFRHGDVIENTRLPGGV
                     WGRYMGSDKRISGAVGAGYTLTQNGMETGGDKVFEFGDSQLAVGAFVSYTDNSVSHNH
                     GGNSKVGSTGGGLYATWFDNDGYYVDGVIKFNRFSNKLRTWMSDGVAVKGDYTQNGFG
                     GSLEAGKTFTLNENAWVQSYVRTTAFRANAKNIRLDNGMAARIEAARSLQGEAGVNLG
                     MNLDVNGVTVKPYLTAAVSHEFSDKNKVRINNSYDFINDISGTTGKYGAGVNAQLTPN
                     AGIWAEAGYQKGNKMESPVTGTVGFRINF"
     gene            1262..1417
                     /locus_tag="RPM73_004218"
                     /pseudo
     CDS             1262..1417
                     /locus_tag="RPM73_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1454..2992)
                     /locus_tag="RPM73_004219"
     CDS             complement(1454..2992)
                     /locus_tag="RPM73_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HDX6522491.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKASRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLVRVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSVLTEEALEQISQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGEREALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
     gene            complement(3042..3388)
                     /gene="tnpB"
                     /locus_tag="RPM73_004220"
                     /pseudo
     CDS             complement(3042..3388)
                     /gene="tnpB"
                     /locus_tag="RPM73_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060295.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(3610..3774)
                     /locus_tag="RPM73_004221"
     CDS             complement(3610..3774)
                     /locus_tag="RPM73_004221"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX6522492.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCDPGISISKLALE
                     NGINANLLFK"
     gene            3859..4155
                     /locus_tag="RPM73_004222"
                     /pseudo
     CDS             3859..4155
                     /locus_tag="RPM73_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a         1105 c         1207 g         1043 t
ORIGIN      
        1 cacctgacca aatcggctga agccgtgctg ggtatggcaa ccgcgccagt ttatattttt
       61 aacagtgaac tgcagaatct acgtttccgt catggtgatg tcatagagaa cacccgttta
      121 ccgggtggag tctggggacg ttatatgggg tcagataaac gcatcagtgg tgcagttggt
      181 gctggctata cgttgacaca gaacggaatg gaaacaggcg gagataaggt ttttgagttt
      241 ggcgacagcc aactggcagt gggtgccttt gtttcctaca ctgataacag tgtcagccat
      301 aatcacggag gaaacagcaa ggttggcagc acgggggggg ggctgtatgc aacatggttt
      361 gataatgacg gttattacgt ggatggggtt atcaaattca accgtttcag caataagctt
      421 cgcacctgga tgagcgacgg tgtggcagtg aaaggtgatt acacccagaa tggttttggc
      481 ggtagtctgg aggcaggtaa aactttcact ctgaatgaaa atgcctgggt acagtcttat
      541 gtacggacaa cggcattcag ggcaaatgca aaaaatatcc gtcttgataa tggtatggca
      601 gccaggatag aagcagccag gtcgcttcag ggagaggccg gagtgaatct gggcatgaat
      661 cttgatgtaa atggcgtgac tgtgaagcct tatctgacag cggcagtgag tcatgagttt
      721 tcggacaaga ataaagtcag gataaacaac agttatgact ttatcaacga tatatcaggt
      781 acgacgggga aatatggggc cggggtgaat gctcagttga cgccaaacgc cggtatatgg
      841 gcagaggctg gctatcagaa agggaataag atggagtcac cagtgacagg aactgtcggt
      901 ttccgcataa acttctgact gtagccagaa ataccagacc gggggagcgt tccgcccccg
      961 gattttagcc tgtacagaga tttgtgtaat tgcctgattt tgagtgagcg taaaccgact
     1021 gccgtatgta accattaagc cagattactg atgcagtgaa gaccttcccg catcctgact
     1081 cacacaacaa tcgactcttt gtgtcctgtc tggacctgtc ggttgccgga agcgccttca
     1141 tgcgaggcgt ctcctcaccg atgcgcgtga ctcaagaagg gcctgacggc ttgtctcgtt
     1201 actgtcctgt ccgggttatc tgtctggaga ttcaactctg tttcctcaca ggagctctgt
     1261 catggctgat aaatataccg aagccgctgt tgtgggtggc gtggatacac ataaagatct
     1321 gcacgttgct gctgtcgtag atgagaacaa taaagtcctt gggacccggt atttctccac
     1381 aacacgacaa ggttaccggc agatgctggc ctggatgtaa gcgtacagcg agggccgtat
     1441 tgacggggat gtgttattca gctggcagtg ctatgcgcca cggaagtagt tcgctgaccc
     1501 ggttgaccgg ccagtctgct atgacgccaa gcacatggcg aaggtagctt tctggatcca
     1561 cgtcattcag tttgcacgtc ccgatcaggc tgtacagtag cgcttcccgc tcaccaccat
     1621 ggtcagagcc gaagaacagg aagtttttac gacccagact gaccgcccgc agggcatttt
     1681 cagcgatgtt gttgtcgatt tccacccagc catcgttcgc atagtacgtc agtgccggcc
     1741 actggttaag tgcgtacgcg aacgccttag ccaactctga gtgtcgcgac agggtcttca
     1801 tcttttcacg caaccagctt tccagggatt tcaacagcgg tttcgttttt cgctgacgtt
     1861 cagcaagccg ctgctctgcc ggcattcccc ttatatccgc ctctatggcg tacaactgac
     1921 tgatctgctc cagggcttct tccgtcagta ctgacgggat gcggacgtgc acatcgtgga
     1981 tctttcggcg ggcatgagcc cagcaggcag cttccgttat cccaccattg cgatacagct
     2041 cgttgaaccc ggcgtacgca tccgcttgca gcacaccgct gaagcaggca agatgagtct
     2101 gcggatggat gccttttctg tccgggctgt aagcgaacca cactgcaggt gccaacgctg
     2161 acccggcatt gcggtcatca cgaacatacg cccacaaccg cccggtcttc gtcttcttat
     2221 tacccggcag cagtacctgg accggggtat catcggcatg gagtttgccg tcagtcatga
     2281 catagccatg aagcgcctct tccagcggag acagcagccg gcagcatgca tccacccagc
     2341 ccgacagcag tgaacgcctc agctccacac cttgccggcc gtatatttct gactggcgat
     2401 acagcggggt gtgctctgca tacttcgagg tcagcactcg gaccagcagc cccggtccgg
     2461 cgataccccg ctcgatgggc cgcgaaggtg caggtgcctg cacgatggca tcgcactgag
     2521 tacaggcatg tttttcccgt accgtccgga taacccggaa ggcgctacgc atcaactcca
     2581 gctgttcggc agtatcctcg cccagatagc tcagtgaacc gccgcagttc gggcagcacg
     2641 gcgccgcagg caacagtcgc ttttcgtcac ggggtagtga ttcagggaac ggcttacggg
     2701 tgcgggtctg acgcaacgga cgctgtactg ccgggtcata caccctacca gtcagcgtat
     2761 cgctctcttt ctgaagccgg ttcagatcgg cttccatttg tgcgatacgg cgggaggctt
     2821 tttcggaacg actgccgaag ttcatccggc ggagtttatc cagctgcgcc tgcagatggt
     2881 ctatttcgcg ctcccggttg ctcagctttt cctgcagggc gtggatcagc gcttcctgtt
     2941 cggccaggcg ctgtttcagc aggaagatgt cgtcagaaga gatgtcgttc ataagcccgt
     3001 attttaccgg gcttattctg tgacaaccag gataaagaga tttacagcat ggtcagggag
     3061 gtcagcagcc gcttaggctg tcgccagtcg ataccttcca gcagcatcgc cagctgcgcc
     3121 tgcgtaagga acactttgcc atcacgggct gacggccagg cgaagcgccc acgctccagc
     3181 cgtttggtca ggaggcacag tccgtcaccg gtggaccaca gcagtttaac ctgactaccg
     3241 ctgcggcccc ggaaaatgaa aacatgccgg acatgggatc gtctttcagc gccgtctgta
     3301 ctttcgcagc caggccgttg aagccatttc tcatatcggt gataccggca accagccaaa
     3361 ttttggtccc ggaaggtaac gggatcatcg cttcagttcc tgtatcagca gagtcaggag
     3421 cttttcgctg acattgccat tgaagcggag cgtcccgtgc cggaacgtta cctcacagct
     3481 gatactgagg gtttccgggt cctctgcgag cgattctggc tgttcggcaa ctgcatcaag
     3541 agtcacagga agtagctggg ggctctctga agaaggtaat agcagctttc cctcgcgcca
     3601 ttgttgtcgt catttgaaca acagattggc gttaatgcca ttttcaagag caagttttga
     3661 gatggatatc ccggggtcac aggaggcagc aacgagctgc tgtttaaatt cgggaggata
     3721 attagggcag ccttttcgcc tgccgggagt cacatttttc tgcatatctg acactttggt
     3781 tcccactact tatttggtgg acaccacttt gtctaattcg tcagattctg accagacggt
     3841 tcaggctgta cgcttacaat tacccagcct gaggatcgtg agctaatgac ccggcatgaa
     3901 atacagaatc gctgtccaga attactgata acgaactact ccatgcttga gtatatgctg
     3961 atgcgaccta tcgagcgtaa tatttttgag cagactaagg aatggctcaa agctgatgag
     4021 atgaatgagc ttatcttagt gcttgatgaa gcgcatatgt atagaggagc agggggagca
     4081 gagatagccc ttttaatacg tcgcctctgt gctctgttgg atattccccg ggaacgtatg
     4141 cgctgcatcc ttaccaggca aaggctcaaa gcattatgaa tgcctggggg tatcataaat
     4201 ctaatgcaag tattggtgac gctcctttgt tgtttggggg aagcgtgccg gggttaacag
     4261 tgaaaggtat ggcgactaat gcagcaattg gtttgagagt caatacaggc gttcaacatg
     4321 atgcagaaac cagcccctag aataaattat tttaacacaa
//
LOCUS       DAPPNJ010000277         4300 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000277 DAPPNJ010000000
VERSION     DAPPNJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.279"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..363
                     /locus_tag="RPM73_004223"
     CDS             <1..363
                     /locus_tag="RPM73_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDX6522493.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="RPM73_004224"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="RPM73_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDX6522494.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="RPM73_004225"
     CDS             2185..3504
                     /locus_tag="RPM73_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDX6522495.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3876..>4300
                     /gene="dosC"
                     /locus_tag="RPM73_004226"
     CDS             3876..>4300
                     /gene="dosC"
                     /locus_tag="RPM73_004226"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDX6522496.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSI"
BASE COUNT         1068 a         1047 c         1045 g         1140 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagcg ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgcact ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaattggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgtct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgccgaa gtggtttccc gctatccggt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac tgctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtaccgg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccgcaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgttttgatt
     3841 ccgtctgatt tcccagcctt ataagggtgt gaattatgga gatgtatttt aaaagaatga
     3901 aagatgagtg gaccggactt gtcgaacagg cagatcctct cattcgtgct aaagccgcag
     3961 aaattgccct tgcgcatgct cattatctga gtatcgagtt ttatcgaatt gtccgcatcg
     4021 acccgcatgc cgaagaattc ttgagtaatg aacaagttga gcggcagttg aaaagtgcga
     4081 tggaacgctg gattattaac gtgctttctg cccaggttga cgatgtcgaa aggctaatac
     4141 aaatccagca taccgtcgcg gaagtgcatg cccgcatagg aattccggta gaaattgtcg
     4201 agatggggtt tcgggtgctg aaaaaaatcc tctatccggt aatcttctct tcggattatt
     4261 ctgccgcaga aaaacttcag gtctaccatt tctcgattaa
//
LOCUS       DAPPNJ010000278         4265 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000278 DAPPNJ010000000
VERSION     DAPPNJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.280"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            226..1158
                     /gene="rfaD"
                     /locus_tag="RPM73_004227"
     CDS             226..1158
                     /gene="rfaD"
                     /locus_tag="RPM73_004227"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDX6522497.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1168..2214
                     /gene="rfaF"
                     /locus_tag="RPM73_004228"
     CDS             1168..2214
                     /gene="rfaF"
                     /locus_tag="RPM73_004228"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDX6522498.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2218..3210
                     /gene="rfaC"
                     /locus_tag="RPM73_004229"
     CDS             2218..3210
                     /gene="rfaC"
                     /locus_tag="RPM73_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDX6522499.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3207..>4265
                     /gene="rfaL"
                     /locus_tag="RPM73_004230"
     CDS             3207..>4265
                     /gene="rfaL"
                     /locus_tag="RPM73_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDX6522500.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGV"
BASE COUNT         1122 a          972 c         1046 g         1125 t
ORIGIN      
        1 aatgcttccg ttatttatca tacatcggaa tattgatact aaagcactat tcacatgcaa
       61 aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc aattagcatc
      121 cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat tacagacatt
      181 cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat catcgttacc
      241 ggcggcgcgg gctttatcgg cagcaacatc gttaaagccc tgaatgataa aggcatcacc
      301 gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct ggtggatctg
      361 aatatcgcag actatatgga taaggaagac ttcctgatcc agattatggc tggcgaagag
      421 ttcggcgatg tcgaagcgat tttccacgaa ggcgcgtgct cttccaccac cgagtgggac
      481 ggcaagtata tgatggataa caactatcaa tactccaagg agctgctgca ctactgcctg
      541 gagcgtgaaa tcccgttcct gtacgcttct tccgcagcca cctacggcgg acgcacctcc
      601 gactttattg aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta ctcaaaattc
      661 ctgtttgatg aatatgttcg ccaaatcctg ccggaagcga actcgcagat tgttggcttc
      721 cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc gagcgtcgct
      781 ttccatctca acacccagct taataacggt gaatcaccga agctgttcga aggtagcgag
      841 aactttaaac gcgacttcgt ctatgttggc gatgtggcag atgtaaacct gtggttcctg
      901 gaaaatggcg tttccggcat cttcaacctc ggcaccggtc gtgcggaatc cttccaggct
      961 gtagctgatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat tccgttcccg
     1021 gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa tctgcgcgcg
     1081 gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata catggcctgg
     1141 ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc gtcttgggtt
     1201 ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta tccccaggcg
     1261 ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat gccggaagtt
     1321 aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga acgccgcaaa
     1381 ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc taactccttc
     1441 aaatccgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg gcgcggcgag
     1501 atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg gccgctaatg
     1561 gtggaacgct atatagcgct ggcctatgac aaaggcatta tgcgcacagc acaagatctg
     1621 ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc atatacctgt
     1681 aatcaatttt cgctttcatc agaacgtccg atgattggtt tttgcccggg tgcggagttt
     1741 ggtccggcaa aacgctggcc acactaccac tatgcggagc tggcaaagca gctgattgat
     1801 gaaggttatc aggtggttct gtttggctcg gcgaaagatc atgaagcagg caatgagatt
     1861 cttgccgctt taaataccga gcagcaggca tggtgtcgga acctggcggg ggaaacacag
     1921 cttgatcaag cggttatcct gattgcagcc tgtaaagcca ttgtcactaa cgattctggc
     1981 ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg tccaagtagc
     2041 ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat taccggctat
     2101 cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat cgacattact
     2161 ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc ctgacggatg
     2221 cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt gcccgcactc
     2281 actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga agaagggttc
     2341 gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc aatacgtcgc
     2401 tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt tcgtgaagcg
     2461 ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa aagcgcggca
     2521 ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac cgctcgcgaa
     2581 cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca gcacgccgta
     2641 gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca aacccagggc
     2701 gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg cgaatatgcc
     2761 gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa
     2821 ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat
     2881 gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag
     2941 atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat
     3001 acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca
     3061 accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg ttctacaagt
     3121 atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct tgaaataata
     3181 accaataagt tgacatcgga gataagatga cctcaacatt atttttctct ctcgagaaaa
     3241 aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc acctattttt
     3301 tgggtgggat tacaaggtat aaacatctta ttgttattct tatgacaata acgacaatcg
     3361 tctatctctg caaacggcca aaacactatc tctcactatt taaaacattt ctttttggta
     3421 gtgttgccat attaactatt gctgcattgc tgtcacttct tcaatcccct gatgcaggtg
     3481 ctagcatgaa ggaaattttc aaagctatta ttgagaatac tttactatgc acaatagcaa
     3541 taccgatcat attgagagac gagaaaagag aagatgtcga aaaaatcgtt ttcttctcat
     3601 ttattagtgc gttgggctta cgctgttttt ctgaattgat tacctattat aaggactatc
     3661 aacaagggat aatgccattc gcagattata gacaccgtag catttctgac tcgatggtct
     3721 ttttattccc tgcattgtta aatctctggc ttatcaaatc agcaaaatac cgcatttctt
     3781 ttgtggttct aagcgttatt tttatttttc tgatattagg aactttatcc agaggggcct
     3841 ggctttccgt gttagtcatt ggattaatat ggattctgat gtttaaacaa tggaagttac
     3901 tattagtagg agtaatggtt gccatcattg cattgtcggt tattttcaca cataaggaga
     3961 tgaccgcaaa gctaacgtat aaacttcaac aaactaatag ttcttatcgc tatgcaaatg
     4021 gtactcaagg cagcgcactc gatctaatat tagaaaatcc tgttattggt tatggttacg
     4081 gtaacgttgc atataaagat gtctataata aacgtgtcat tgattatcca gaatggacct
     4141 ttagacaatc aatagggcca cataattttg cgctattcat ctggtttggc actggtttat
     4201 tagggctggt aagtcttatg atgctatact gtgcaatatt gaaagagtgt ataaaaaatg
     4261 gcgtc
//
LOCUS       DAPPNJ010000279         4230 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000279 DAPPNJ010000000
VERSION     DAPPNJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.281"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..452)
                     /locus_tag="RPM73_004231"
     CDS             complement(<1..452)
                     /locus_tag="RPM73_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDX6522501.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPD"
     gene            complement(597..1946)
                     /gene="lysC"
                     /locus_tag="RPM73_004232"
     CDS             complement(597..1946)
                     /gene="lysC"
                     /locus_tag="RPM73_004232"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDX6522502.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            2471..4120
                     /gene="pgi"
                     /locus_tag="RPM73_004233"
     CDS             2471..4120
                     /gene="pgi"
                     /locus_tag="RPM73_004233"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDX6522503.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1090 a         1072 c         1053 g         1015 t
ORIGIN      
        1 aaatcagggt gtagaaatcg aaaatcatca ccgttaataa tcattatatt atcttttgtc
       61 tctcttttgg ctaattcaat cagagatgat ttacctgctc cgggttgccc acctaaaaca
      121 aaaccaatgg gatgctgttg cggtgtgact tcatgatttg tcagacgctg ccaaatagaa
      181 tcaaaagatg ctttattttc tgaatgcgta gtattgttga attcatgctg atattgagtt
      241 cttacctgct gaaggagaat cttaccttgt gtaaaatcaa caagttcagc gggggtagaa
      301 taaagataat cgcgatagct gactaatcgc ttgagagcat cgcctgaatc ctgatgcgac
      361 gaaactgagc aattatcaat gatatttgat aaaaacgcga tgcactgact ggctccgttt
      421 aaaagtgcat ctttttccat taaagtagcc attaacactc ctgttaaatg atatttttcg
      481 gtgattataa tactgttcta caggagtgat tttgcttttc agatattatt taatctatgt
      541 tttcagtgag gatggcgtat aacaatcggt gagcgttata cgccagtagg gaaatattac
      601 tcaaacaaat taaaatgcag cttctgcacc acctgctcgg catcttcgcc gggcaccagg
      661 aagcacaggt tatggctgga tgcgccgtaa caaatcatgc gaatgttgaa cggttccagt
      721 acgccgaata cctctttgcc aacgccgcag gcttttgaca ggtcattgcc aatcaacgcg
      781 accagcgcca gaccttcttc tacttccacc cggcacagtg cggaaagctc catcagcaga
      841 gattgcgtca gcaacgtatc gccagtggag gttgaaccgg tggtatcaag ggttaatgcc
      901 acgctcactt ctgacgtggt gattaagtct accgaaatat tatgccgcgc gaggatgccg
      961 aaaacttccg cgaggaagcc gcgagaatgc agcatattca ggctgtgcaa agtgagcaga
     1021 gtctgattgc gacgaagcgc cagcgcgcgg aacagcggcg gattttcagt tttattgcac
     1081 accagcgtac cacctgcgcg tgggtctttg ctggagccga caaagaccgg gatatcgctg
     1141 cgtactgcgg gtagcaacgt tgccggatgc agtacttttg caccaaaagt tgccatctct
     1201 gccgcttcgg caaacgcgat ttcatcaatg cgttttgctg cggaaactac gcgtggatcg
     1261 gtggtgtaga tgcccgggac gtcggtccag atatcaacac gagatgcgtg taaagcctcc
     1321 gccagcaagg ctgccgtata atcgctgcct ccacggccaa gcgtcgttgt acgaccttta
     1381 ttttcgctac cgataaatcc ctgggtgatc actaagccgt cattgaggcg tgggagcagc
     1441 tgcagcgcgg ccagttccgc cagcgcggct acatctggct ctgcacgacc aaatcggtcg
     1501 ttggtacgca tcactttacg tacatcaaac cactgtgcct gaacatcgcg ttcgcgcagg
     1561 atttcgacaa acagcagggt cgacatcagc tcgccgtggc tgaccagctc atctgtcagc
     1621 gccggagacg ttgccagcgc cgccgcttct gccagaacag taatgttctc cagcagacgt
     1681 tcaatctctt cacggataac gttcgggtaa cgcagacgtt ccagaatggc aaactggatg
     1741 ttgcgaatag cgtcgagttt ttcgaatcgc tcgccaggtt ccagcccttc cgctaaagcg
     1801 accagcagat tagtgatacc agcagaagcc gagaggacta ctaaacgcac gttggcatca
     1861 gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat cagctacgct ggtaccgcca
     1921 aatttggaga caacaatttc agacataact acctcgtgtc aggggatcca ttttcagcct
     1981 tggcacaagg gaagagcgga agacgggtgg gcgcagagcg atacttcgct actattttca
     2041 cccagaagtg ctccaccact tgcgaaacgc ccgactgcga acgcttctgg tgacaaccca
     2101 ggggattcag cccctgtagc cgatgatgaa cgtggccagc cgttcaatca cctcggcgat
     2161 gcaccccctc aggtgttatc acaggactgg ctcctccaac accgttactt gggcaacgcg
     2221 cctcttctgg cctgcgctag cgcaggtagt acatttataa ataaagggtg agcggggcgg
     2281 ttgtcaacga cggggtcatg cggatttttc atccactcct ggcggtcagt agttcagcta
     2341 ataaatgctt cactgcgcta agggtttaca ctcaacatta cgctaacggc actaaaacca
     2401 tcacattttt ctgtgactgg cgctacaatc ttccaaagtc acaattctca aaatcagaag
     2461 agtattgcta atgaaaaaca tcaatccaac gcagaccgct gcctggcagg cactacagaa
     2521 acacttcgat gaaatgaaag acgttacgat cgccgatctt tttgctaaag acggcgatcg
     2581 tttttctaag ttctccgcaa ccttcggcga tcagatgctg gtggattact ccaaaaaccg
     2641 catcactgaa gagacgctgg cgaaattaca agatctggcg aaagagtgcg atctggcggg
     2701 cgcgattaag tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc gcgctgtgct
     2761 gcacgtagcg ctgcgtaacc gtagcaatac cccgattttg gttgatggca aagacgtaat
     2821 gccggaagtc aacgcggtgc tggagaagat gaaaaccttc tcggaagcga ttatctccgg
     2881 tgagtggaag ggttataccg gcaaagcaat cactgacgta gtgaacatcg ggatcggcgg
     2941 ttctgacctc ggcccgtaca tggtgaccga agcactgcgt ccgtacaaaa atcacctgaa
     3001 catgcacttt gtttctaacg tcgatgggac tcacatcgcg gaagtgctga aaaaagtaaa
     3061 cccggaaacc acgctgttcc tggtagcatc taaaaccttc accactcagg aaaccatgac
     3121 caacgcccat agcgcgcgtg actggttcct gaaagcggca ggtgatgaaa aacacgttgc
     3181 gaaacacttt gcggcgctct caaccaatgc caaagccgtt ggcgagtttg gtattgatac
     3241 tgccaacatg ttcgagttct gggactgggt cggcggccgt tactctttgt ggtcagcgat
     3301 tggcctgtcg attgttctct ccatcggctt tgataacttc gttgaactgc tttctggcgc
     3361 acacgcgatg gacaagcatt tctccaccac gcctgccgag aaaaacctgc ctgtactgct
     3421 ggcgctgatt ggcatctggt acaacaattt ctttggtgcg gaaactgagg cgattctgcc
     3481 gtatgaccag tatatgcacc gtttcgcggc gtacttccag cagggcaata tggagtccaa
     3541 cggtaagtat gttgaccgta acggtaacgt tgtggattac cagactggcc cgattatctg
     3601 gggtgaacca ggcactaacg gtcagcacgc gttctaccag ctgatccacc agggaaccaa
     3661 aatggtaccg tgcgatttca tcgctccggc tatcacccat aacccgctct ctgatcatca
     3721 ccagaaactg ctgtctaact tcttcgccca gaccgaagcg ctggcgtttg gtaaatcccg
     3781 cgaagtggtt gagcaggaat atcgtgatca gggtaaagat ccggcaacgc ttgactacgt
     3841 ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac tccatcctgc tgcgcgaaat
     3901 cactccgttc agcctggggg cgttgattgc gctgtatgag cacaaaatct ttactcaggg
     3961 cgtgattctg aacatcttca ccttcgacca gtggggcgtg gagctgggta aacagctggc
     4021 gaaccgtatt ctgccagagc tgaaagatga taaagaaatc agcagccacg atagctcgac
     4081 caatggtctg attaaccgct ataaagcgtg gcgcggttaa tttaaaaacc ttaataacaa
     4141 tgccgacgtt atgtcggcat tgttttatca gataaatccc cttgtctgtg atttaacgga
     4201 aatcataccg tgaggttaat cctaaaatag
//
LOCUS       DAPPNJ010000280         4200 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000280 DAPPNJ010000000
VERSION     DAPPNJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.282"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            42..1046
                     /gene="yeeE"
                     /locus_tag="RPM73_004234"
     CDS             42..1046
                     /gene="yeeE"
                     /locus_tag="RPM73_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDX6522504.1"
                     /translation="MQRGRFCLTGGFRDMYLAKNNRMFYALLIAISVQSVGVFALIQA
                     GLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMS
                     AVMRSPHASGLNQTLQHYSTEHNSIAETFHLSVWPLVAVLLVITLWVVMKELKKPKLK
                     VATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANI
                     LQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGF
                     GASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN
                     "
     gene            1060..1287
                     /locus_tag="RPM73_004235"
     CDS             1060..1287
                     /locus_tag="RPM73_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDX6522505.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1330..2757)
                     /gene="sbcB"
                     /locus_tag="RPM73_004236"
     CDS             complement(1330..2757)
                     /gene="sbcB"
                     /locus_tag="RPM73_004236"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDX6522506.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2966..4132
                     /gene="dacD"
                     /locus_tag="RPM73_004237"
     CDS             2966..4132
                     /gene="dacD"
                     /locus_tag="RPM73_004237"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDX6522507.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
BASE COUNT          984 a          975 c         1126 g         1115 t
ORIGIN      
        1 tattaagcgg gctaatttgt ggtgctctgc tgggatttgt catgcagcgt gggcgttttt
       61 gcctgacagg tggttttcgc gatatgtatc tcgcgaaaaa taatcgcatg ttttacgcct
      121 tgctgattgc aatttcggta caaagtgtgg gggtttttgc gttaattcag gcgggcctac
      181 tgacttacga agccggggcg ttcccgtggc ttggtactgt tataggtggg tatatctttg
      241 ggctgggaat tgttctggcg ggtggatgtg ccaccgggac ctggtatcgc gcgggtgagg
      301 gattgatcgg cagttggatc gcgcttttca cttatatggt gatgagtgcg gtgatgcgtt
      361 ctccacatgc cagtggttta aatcaaacct tgcagcacta cagtactgaa cataactcta
      421 ttgctgagac ttttcatttg tctgtgtggc cgttggttgc cgttttgctg gtgataacgc
      481 tctgggtggt gatgaaagag ttgaagaagc caaaactcaa agtcgcgacc ttaccgccgc
      541 gccgaaccgg gatcgctcat attctgtttg aaaaacgctg gcatcccttt gtcactgctg
      601 tactcatcgg tttgattgcg cttttagcct ggcccctgag tgaagcaacc ggacgcatgt
      661 ttgggttagg aatcacttcc ccaacggcca atattctgca atttctggtc gcgggtgacg
      721 tgaaatacat taactggggc gttttcctgg tgttagggat cttcgtgggg tcatttattg
      781 cagccaaagc gagccgtgag ttccgcgttc gcgcagctga tgcacaaaca acattacgta
      841 gcgggttagg tggtgtactg atgggcttcg gtgccagtat tgcaggtggt tgctctatcg
      901 gtaatggact ggttatgact gcaatgatga cctggcaggg ctggattggc cttgtattta
      961 tgattctcgg agtctggact gcgtcctggc ttgtgtatgt tcgaccgcag cgtaaggcgc
     1021 gactggcaac tgctgcggca aattaagtaa ggtgtaaaga tggcgattaa aaagctggat
     1081 gtggtgacgc aggtctgtcc gtttccactc atagaagcaa aagcagcact ggcagaaatg
     1141 gccagcggcg atgagttggt aattgagttt gattgcaccc aggcaactga agcaatccca
     1201 cagtgggcgg cagaagaagg acatgccatc accgattatc agcagattgg cgatgcagcg
     1261 tggagcatca ccgttcaaaa agcctgactc aacattgtcc tccgccgtac cagcggcgga
     1321 ggcttcaaat tagacgatct cttccgcgta ctgccaaagc gcttttaaca gcgccacttt
     1381 ctctttgtca tcggcatatt gttgtgccag catctgcaat tcatctgcat aaccctgtaa
     1441 aaactctggc gtgaagacct ggcggcggtg ctccagccag cgttgctgct cggcataatc
     1501 cagcgtcccc gggaagttgc gcgcccgata attgaacaac agcttttcaa tccgtttatc
     1561 gacaaaagtg atatccagtg ccggtaaatt acgcggctcg gtttccagca caattttcat
     1621 tgctgcacga tcggcgtcac tgaaaaagcc gttataaagc tgtgcatcca cgttatctga
     1681 aggcgtaaac ggttcggctt ccgcgaatat cgccaccact ttttcgcgca cttgcggatt
     1741 ttcacgcaga attttcaggt tatcgaggca atgctgacga ttaattccca gtcggtcggc
     1801 atcttccggg cgtagcgtat tcgcctgggc cagcaccgga catttattga tatgcaccag
     1861 cttaaccgga acggcggcgt tatcgccaag atcggttttt gcggtatata aacgctcgcg
     1921 caatgtgtcg ctatccagtt ccagtaatgg cgaaatgtct cctgccaaat ccaccataat
     1981 tacggcattg cgattttcag gatgccacgc cagcggtgcc acccagctgg tattgccgcg
     2041 ccatgctcca aacattccgg aaacgtgcac caggggtttc atctgcggaa catcgatcaa
     2101 cgccatcagt ttgtgtttat tacgatgggt aaagagataa tcaaacaggc gtggctgacg
     2161 cgtttttacc agctttgcca tcgcaatagt ggcgtacaca tcagccatcg catcgtgggc
     2221 gttgctatgt tcaataccat tcgctttggt taaatgctca aggcgaaagc tcggtagacc
     2281 gtcatcattt tcaggccagt ttattccttc cgggcgcagg gcataacagg cacgcataac
     2341 atccagcaaa tcccagcgcg agttatcatg ctgccagctc caggcgtaag gatcgtagaa
     2401 attacgataa aaaacgttgc gtgtgacttc gtcgtcgaaa cgcacattgt tgtagcccag
     2461 aatacaggtc ttcggtacgg taaaaagcga gtgaatacgg gcggcaaacg cggcttcgtt
     2521 ttctcctttc gcccgtgctt cctgcggggt aataccggta attaatacgg ctccaggctg
     2581 gggtaaatag tcatccgcgg gcttgcagta aaagacttcg ggttcgccga tgacattgaa
     2641 ttcgctatcg gtgcgaatgg ctgcgaactg tgcagggcga tctaacgcgg ggtgcgtgcc
     2701 aaaggtttcg taatcgtgaa acaaaaaggt agattgttgc ttaccgtcat tcatcattag
     2761 gttaaatccg ttatttctgc tgtctgccag agtatcaaat atcaccgtgc taatcagctt
     2821 tagcgcaaca atttgacagc gagtggcaac agatcatgtc agataaaaat gagagagtag
     2881 tcacattttc ttgcacttta ttccagccag ttcataagta tttccgtaaa aagaacagct
     2941 atttgaaact cctgagggtt tgctgttgaa acgccgtctt attattgctg cttctttgtt
     3001 cgtttttaac ttatcgtctg gttttgcggc ggaaaacatt cctttttcac ctcagcctcc
     3061 acagattcat gccgggtcct gggtactgat ggattacacc accggtcaga tcctcaccgc
     3121 gggtaatgag catcaacagc gcaatcccgc cagcctgaca aagctgatga cgggttatgt
     3181 cgtggatcgc gctatcgata gtcatcgcat tacgccagac gatattgtca ccgtggggcg
     3241 cgatgcgtgg gcgaaagata atccggtgtt tgtcggttct tcactgatgt ttttgaaaga
     3301 gggcgatcgc gtatcggtac gtgatttaag ccgtggttta attgtggatt ccggaaatga
     3361 cgcttgtgtt gctctggctg actatattgc cggtgggcaa cggcagtttg ttgaaatgat
     3421 gaacaactat gccgagaagc tgcatctcaa ggatacgcat tttgaaacag tgcatggtct
     3481 ggatgcacct ggccagcata gctcggctta tgatttagct gtgctttctc gcgctatcat
     3541 ccacggcgag cccgagtttt atcatatgta cagtgagaaa agtctcacct ggaacggtat
     3601 cacccagcaa aaccgtaacg ggttgttgtg ggataaaacc atgaatgttg acggcctgaa
     3661 aacgggtcat acttctggtg ccggttttaa cctcattgct tcggctgtag atgggcagcg
     3721 tcgtctcatt gcagtggtaa tgggggctga cagtgcaaaa ggtcgtgagg aagaggcaag
     3781 aaaattactg cgttgggggc aacaaaactt tactacggtg caaattttgc accgtgggaa
     3841 aaaggtcgga acggaacgca tctggtatgg cgataaagaa aatatcgacc tgggaacgga
     3901 acaagagttc tggatggtgc taccgaaagc cgaaattcca catatcaaag ccaaatatac
     3961 ccttgatggt aaagagctca ccgcgccaat tagcgcccat cagcgggtag gggaaattga
     4021 actttacgac cgtgataaac aggtggcgca ctggccgctg gttaccctgg aatctgtcgg
     4081 ggaaggcggc atgttttccc gcctgagtga ttatttccac cataaggcct gacctttctt
     4141 ttgcagcaga ctggcaggag tgcgagtctg ctcgcataat caacactcat tccttgtggt
//
LOCUS       DAPPNJ010000281         4179 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000281 DAPPNJ010000000
VERSION     DAPPNJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.283"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..3027
                     /gene="evgS"
                     /locus_tag="RPM73_004238"
     CDS             <1..3027
                     /gene="evgS"
                     /locus_tag="RPM73_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDX6522508.1"
                     /translation="SFTNLYQALASVSAGHNDYFIGSNIITSSMISRYFTHSLNVVKY
                     YNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELT
                     EHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVS
                     HNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKG
                     MKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELDALVATQLNSRYMIDH
                     YYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPN
                     VTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKAL
                     SDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSNAHEVT
                     AETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYICGWQDITETRDLINA
                     LEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAY
                     ATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCS
                     STFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMD
                     SGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTT
                     FTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDE
                     ATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANER
                     EKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQ
                     EILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEIT
                     PVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3083..>4179)
                     /gene="yfdE"
                     /locus_tag="RPM73_004239"
     CDS             complement(3083..>4179)
                     /gene="yfdE"
                     /locus_tag="RPM73_004239"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDX6522509.1"
                     /translation="MTHVLNGPFGTQLLCNMGARVIKVEPPGHGDDTRTFGPYVDGQS
                     LYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFRPGTMEKLGFSWETLQEI
                     NPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGG
                     VYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPY
                     MAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQY
                     IERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMPGNPI
                     KISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1260 a          921 c          843 g         1155 t
ORIGIN      
        1 tcttttacaa atttatatca ggcattagca tccgtctcag ctgggcacaa tgattatttt
       61 attggtagta acatcattac cagcagtatg atttcccgct atttcactca ctccttaaat
      121 gtagtgaaat attataactc gccgcgtcaa tataattttt tcttgaccag aaaagaatct
      181 gtcattctta atgaagtact caatagattt gttgatgctt taacaaatga agttcgctat
      241 gaagtatcac aaaattggct tgatacagga aacctggcct ttctgaacaa accattagaa
      301 ctcactgaac atgaaaaaca gtggattaag cagcatccca atttaaaggt gctggaaaat
      361 ccttactcgc ccccctattc tatgacggat gaaaatggct cggttcgggg cgttatgggg
      421 gacattctta atattattac cttgcaaaca ggtttaaatt tttctccgat caccgtttca
      481 cacaatatcc atgctggaac acagcttagc cccggaggat gggatataat acctggcgct
      541 atttatagtg aagatcgaga aaataatgtt ttatttgctg aagccttcat aacaacgcct
      601 tacgtttttg tcatgcaaaa agcgcctgac agtgaacaaa cattaaaaaa aggaatgaaa
      661 gttgccattc catattatta tgagctgcat tcgcaattaa aagagatgta tccggaggtt
      721 gaatggatac aggtcgataa tgccagcgct gcatttcaca aggttaagga aggtgaactt
      781 gatgctctgg tcgcgacaca gttaaattcg cgttacatga tcgatcatta ctatcctaat
      841 gaactttatc attttcttat tcctggcgtt ccgaatgcat cgctttcgtt cgcttttcct
      901 cgcggagaac cggaacttaa ggatattatt aataaagcac tgaatgcaat tcccccaagc
      961 gaagttctgc gcctgacgga aaaatggatt aaaatgccca atgtgaccat tgacacatgg
     1021 gacctatata gcgagcaatt ttatattgtt acgacattat ccgttttatt agttggcagt
     1081 agccttttat ggggattcta cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt
     1141 gatttagaaa accaaatatc attccgaaaa gcactctcgg attccttacc gaatccaact
     1201 tatgttgtaa actggcaagg taatgtcatt agtcataata gtgcttttga acattatttc
     1261 actgcggatt actacaaaaa tgcaatgtta ccattagaaa acagtgactc accctttaaa
     1321 gatgtttttt ctaatgcgca tgaagtcaca gcagaaacga aagaaaatcg aacaatatac
     1381 acacaggtat ttgaaattga taatggcatc gagaaaagat gcattaatca ctggcataca
     1441 ttatgcaatc ttcctgcaag tgacaatgca gtatatattt gtggttggca agatattact
     1501 gaaacgcgtg atctaattaa tgcactcgag gtagaaaaaa ataaagcgat aaaggctacc
     1561 gtagcaaaaa gtcagtttct ggcaacgatg agtcacgaaa taagaacacc aataagctct
     1621 attatgggct tcctggaact tctgtcgggt tctggtctta gcaaggagca acgggtggag
     1681 gcgatttcac ttgcctacgc caccggacaa tcactcctcg gcttaattgg tgaaatcctt
     1741 gatgtcgaca aaattgaatc gggtaactat caacttcaac cacaatgggt cgatatccct
     1801 actttagtcc agaacacttg tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca
     1861 ttaagttgca gcagtacatt tcctgatcat tacctggtta agatcgaccc tcaggcgttt
     1921 aagcaggtct tatcaaattt actgagcaat gctctcaaat ttaccaccga gggggcagta
     1981 aaaattacga cctccctggt tcacattgat gacaaccacg ctgttatcaa aatgacgatt
     2041 atggattctg gaagtggatt atcgcaggaa gaacaacaac aactctttaa acgctacagc
     2101 caaacaagtg caggtcgcca gcaaacagga tctggcttag gcttaatgat ctgcaaagaa
     2161 ttaattaaaa atatgcaggg cgatttgtca ttagaaagtc atccaggcat aggaacaaca
     2221 tttacgatca caatcccggt agaaattagc cagcaagtgg cgactgtcga ggcaaaagca
     2281 gaacacccca tcacactacc tgaaaagttg agcatattaa tcgcggatga tcatccgacc
     2341 aacaggctat tactcaaacg ccagctaaat ctattaggat atgatgttga tgaagccact
     2401 gatggtgtgc aagcgctaca caaagtcagt atgcaacatt atgatctgct tattactgac
     2461 gttaatatgc cgaatatgga tggttttgag ttgactcgca aactccgtga gcaaaattct
     2521 tccttaccca tctgggggct tacagccaac gcacaggcta acgaacgtga aaaagggtta
     2581 agttgcggca tgaacttatg tttgttcaaa ccgttgaccc tggatgtact gaaaacacat
     2641 ttaagtcagt tacaccaggt tgcgcatatt gcacctcagt atcgccacct tgatatcgaa
     2701 gccctgaaaa ataatacggc gaacgatcta caactgatgc aggagattct catgactttc
     2761 cagcatgaaa cgcataaaga tctacccgct gcgtttcaag cactagaagc tggcgataac
     2821 agaactttcc atcagtgtat tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa
     2881 aagttgatta atattagcca tcagttagaa ataacacctg tttcagatga cagtaagcct
     2941 gaaattcttc agttgctgaa ctctgtaaaa gaacacattg cagagctgga ccaggagatt
     3001 gctgttttct gtcagaaaaa tgactaaata gcggctccca caatgttcaa atgtgggagc
     3061 tatttaccag cacatcttac atttatgatg agaactcctg gcgaatttgt tccccatgct
     3121 ggtcgagcgt tgccgctccc ggcataacat gcgggtccgc gcagccgctg atttttatcg
     3181 gattacccgg catcattatt cccccggctt caatcaacat atttctcgcc tgagtttgtg
     3241 gcaatttaat ggcctcagcc acacttaata acggcgcgac gggtacacca acttcgtgta
     3301 ttctggctaa ccaaacttca gctgcctgcg tttttaacgt ccgctcaata tattgtttaa
     3361 gaatagcctg gttttgtacg cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt
     3421 ccgtaagctc cagtgcctgg cataacgcag aaaaaagctt gtcattacca caacaaatcg
     3481 taatcggctt atcctgagta ttgaaaacat caaaaggtgc catgtaggga tggcgatttc
     3541 ccagacgttg tggtgacttc ccagttgcga tatatgccat cagaccatgc tccagaaaac
     3601 tcagcgtggc atcaaacatc gctatatcga catgcgcccc tctctggctc ttttcgcggc
     3661 cataaagtgc actcactatt ccgctgaata aatagacacc gccgcataga tccgcaagag
     3721 aggtaccaac gcgcactggc ggagcatcag gatatcctgt ttccatcatt atcccgctca
     3781 ttgcctgaat gatggtatcg taggcaggag catcttttag cggaccggta tgtccgaaac
     3841 ctgacgatga agcatatatg aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc
     3901 ccagtttttc cattgtacct gggcgaaaat tctcagctaa tacatcagct tgtttgagca
     3961 tatttataaa tatactttta tcgtgatcat tctttaaatc aagaaccaca ctctctttgc
     4021 catgattaat aaaactgtaa tagagtgact gtccatccac atagggacca aatgtgcggg
     4081 tatcatcacc atgacccggc ggctcaactt taattaccct tgcgcccata ttacaaagaa
     4141 gttgagttcc gaaaggtcca ttaaggacat gtgtcatat
//
LOCUS       DAPPNJ010000282         4177 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000282 DAPPNJ010000000
VERSION     DAPPNJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.284"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(81..1337)
                     /gene="xapB"
                     /locus_tag="RPM73_004240"
     CDS             complement(81..1337)
                     /gene="xapB"
                     /locus_tag="RPM73_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDX6522510.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFTGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(1397..2230)
                     /gene="xapA"
                     /locus_tag="RPM73_004241"
     CDS             complement(1397..2230)
                     /gene="xapA"
                     /locus_tag="RPM73_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDX6522511.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2479..3243
                     /locus_tag="RPM73_004242"
     CDS             2479..3243
                     /locus_tag="RPM73_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522512.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3282..4130)
                     /locus_tag="RPM73_004243"
     CDS             complement(3282..4130)
                     /locus_tag="RPM73_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6522513.1"
                     /translation="MGLSQSAVSHSVKELENHTGVRLLDRTTREVVLTDAGQQLALRL
                     ERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQCIAESHRRYPDIQFVLHD
                     RPQQWVMESIRQGDVDFGIVIDPGPVGDLQCEAILSEPFFLLCHRDSALAVEDYVPWQ
                     ALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPATLFPMVAAGIGISILPA
                     LALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVRDQGNALMAGREG
                     DPLYQI"
BASE COUNT         1150 a         1108 c          939 g          980 t
ORIGIN      
        1 cgccggaaga aaataaaata tctttcacta attaaccttt atcataaaag cagctctgaa
       61 gagcagagct gcgaatcctt ttaatgagcc accgctcgat gctttatctt ttcagggtca
      121 tgattatatt taaacccaaa gaaaaatatc actgcgagaa aaagagcata tcctgtaaac
      181 accagccaga tagtttgcca gtcttttacg ccatccaccg aaaagtaatc tactgccatg
      241 ccactcagaa tcgagccaac ccatgcgccg acaccattta ccatggtcat aaagagcccc
      301 tgcgcgctgg cacgaatgct ggaatcaact tcctgttcga caaataccga accagaaata
      361 ttgaagaaat cgaatgcaca gccataaaca atcatcgaca gcagcagcaa aataaatccg
      421 gttgttgacg gatcgccata ggcgaagaag ccaaagcgca gcgtccaggc caccatactc
      481 atcagcatga cggctttaat gccaaatcgc tttaaaaaga atgggatagt cagtataaag
      541 cccacttctg ccatctgtga aactgacagt aaaatggagg gatatttcac cacaaaactg
      601 tcagcaaact ccgggttacg ggcgaaatca tgtaggaacg gattaccaaa aacgttggta
      661 atttgcagta ccgcacccag catcatggca aagaggaaaa agatggccat gcgtggattt
      721 ttaaacagca cgaaggcatc cagacccagc ttgctggcaa gcgatgtggt cgcttttttc
      781 tccgcaaccg gaatcttcgg caaagtcagc gcataagccg acagcagcaa tgacgcaccg
      841 gacgcgatat acagctgcag actactcaat tccagatgca gcaggcttac tgcccacatc
      901 gcgacaatga accccaccgt accaaaaacg cgaatgggcg ggaaagcggt caccgggtca
      961 agccctgcct gggcaagaca ggaataagag acgctgttcg ataacgcaat agtcggcata
     1021 aacgccatcg cattgactaa catcacccaa aacatcatat ccggatcagt tacggatgcc
     1081 gcataaaaaa gtacgcccgc acacaccagg tgacacagca tgtatgcacg ttctgcgcgc
     1141 agccatttgt ctgcgatgat ccccattata ccaggcataa taatcgcggc gatccctttg
     1201 gaactgtaaa ccatgccaac attagcgccg gtgaaatgaa gggtattaat catgtaagag
     1261 ccgagggtaa ccagccagct cccccagata aaatattgca aaaaggacat tacctttaag
     1321 cgcatcgcga tgctcataat cgtttccttg tcgcatcatt tggcctcacc cgcgaggccg
     1381 tttcttattg tttttctcag gcaattttgc gcagaaagcc acaaataagg ttgatgaagt
     1441 tctgcttaga gagctccgca gccgccagcg tttgcgcatg tgacaatttc acatcgctta
     1501 aaccttccgc catattggta atcgcagaga ccgcaacgac tttaaggtcg caatggcgag
     1561 ctgaaataac ctcaggcacc acagacatac caacaacatc cccaccaata atttgcatca
     1621 tgcgaatttc cgccgcagtc tcgaaattcg gccccggata cgagacgaac acgccctccg
     1681 tcagagggaa accctcttct ttcgccactt tttgtaacag tgcgcggtat tccgcatcgt
     1741 aggcatttgc cagtgagaag aagcgctctc caaaacgatc atcgttaaga cccaccatcg
     1801 gcgtaccggg catggtgttg atatgatctt tcaatgcgac caggctgcct gcccccactt
     1861 ccggacgcag cgagcctgcc gcattggtgc agaacagtag ctcgcagccc agcagcttaa
     1921 aggtacgaat tgcgtcagtc ataatggtca ttccacgacc ttcgtagaaa tgtccgcgac
     1981 ctttcataca taccaccggt acgccctgga gatggcccag cactaactcc cccgcatgac
     2041 catgtaccgt acttaccggg aagcccggca gcttttcgta ggaaattgcg acagcgttct
     2101 caatctgatc ggccagcgcg ccgagcccgg aacctaaaat aaaggccact cgtggcgtga
     2161 aatcaggttt ataagtcttg ataatatcta tgcaaaacag cgggttatga gaaaattgaa
     2221 cctgagacat acatatcctt tttctgtagg gtggaatcta acgccttttt atagcgaggt
     2281 tgtcccggct cttaccaata cacttttctc acgcattcaa tagcgattcg atatcgatgg
     2341 atttatatct tttctctatt aatccacggt aaaaactgta ttggcgatat taattccttt
     2401 gcattaacaa tatgtcgttt ttagcaaacc actttattaa gcaatctact aataacgatc
     2461 ggcataggga attatgctat gaaaaaacat cttttaactc tgacactttc ctctatatta
     2521 gcgatacctg ttgtatctca tgcacaattt aaaggcggtt ttgcagatat cggcgtgcat
     2581 tatctggact ggaccagccg gactaccgaa aaatcgtcga ccaaatcaca caaagatgat
     2641 tttggttatc ttgaatttga aggtggcgct aactttagtt ggggagaaat gtacgggttc
     2701 ttcgactggg aaaacttcta taacggtcgc cataataaac caggtagcga gcagcgttat
     2761 acctttaaaa ataccaaccg tatttacctg ggtgacaccg gatttaatct ctacttgcat
     2821 gcgtatggca cctacggttc tgcgaatcgc gtgaatttcc acgacgatat gttcctgtac
     2881 ggtatcggct acaatttcac cggcagcggt tggtggttca aaccgttctt tgctaagcgt
     2941 tatacagatc aaacctatta cactggcgac aacggctatg tcgccggctg ggttgcaggt
     3001 tacaacttta tgctgggcag cgagaaattc actcttacca actggaacga gtacgagttt
     3061 gaccgtgacg ctacctatgc agcgggtaat ggcggtaaag aagggctgaa tggcgcagtt
     3121 gcactgtggt ggaatgcaac atcacacatt actacgggga ttcagtatcg atatgcggat
     3181 gacaaactgg gcgaagattt ttaccaggat gcgataatct actccatcaa atttaacttc
     3241 tgattgatta ctgccggggc tcccggcagt atgaattcac tctatatctg atacaacgga
     3301 tccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
     3361 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca
     3421 accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag cgccagtgcg
     3481 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     3541 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     3601 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     3661 acataatctt cgacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
     3721 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     3781 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac
     3841 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     3901 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     3961 tcgcgcaacg tgctgttcag ttcatccagc agtcgctcaa gacgcaaagc cagctgctgc
     4021 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     4081 ttttccagtt ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct
     4141 gcacgactaa aacttttctc ctgcgctact gtgacga
//
LOCUS       DAPPNJ010000283         4142 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000283 DAPPNJ010000000
VERSION     DAPPNJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.285"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..768)
                     /locus_tag="RPM73_004244"
     CDS             complement(<1..768)
                     /locus_tag="RPM73_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX6522514.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDG"
     gene            1087..2364
                     /locus_tag="RPM73_004245"
     CDS             1087..2364
                     /locus_tag="RPM73_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6522515.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2391..3869
                     /locus_tag="RPM73_004246"
     CDS             2391..3869
                     /locus_tag="RPM73_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX6522516.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPSCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1083 a          941 c          997 g         1121 t
ORIGIN      
        1 accatcgacc actaataaat gcccattgac ataatttgaa gccggacttg cgaggaatac
       61 agctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
      121 gcgctgattg gtttctggat tactgcgtgt cgccagcgta atatctgttg cataatagcc
      181 aggggcgata ccatttacct gaatattata ttgacctagc tcatcacaat aagctttggt
      241 gaacccggca agagcatgtt tagtggcaga atatgcaggt gaccattgtc cacctaagta
      301 agagaacaat gaacagatat taataatttt gccgcttttc tgcgggatca taatttttgc
      361 agcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg gatcccagtc
      421 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt ttaccagaat
      481 atcaactgta ccgaaacgct cacagcaagc agcgataatc ttctgcggcg cgccttctgc
      541 ggtgatatcc acctgcatga agtccacctc aacaccctgt ttttcaatca tttcctttgt
      601 ttcgccgtta tctttgacga aactaggaat aaagatattt gcgccagctt tggccaacgc
      661 catggcaaat gcctggccta aaccgctatt cccaccggta acaattgcgg ttttaccttt
      721 cagtgagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
      781 tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
      841 cattatctct ggtaactgga gataattttt agcataacgg cataactgct aatgtgatcg
      901 taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc gtgattactc
      961 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
     1021 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
     1081 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
     1141 ggcggcgtta gtttcgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
     1201 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
     1261 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
     1321 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
     1381 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
     1441 gtgtcgatta aagccgcctc gttgctgggc gatcatagtg agcaagggaa aattatgggc
     1501 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
     1561 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat catctacagt
     1621 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
     1681 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
     1741 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac aatctacgcc
     1801 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcagcg
     1861 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggcggc
     1921 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgatccaaat cctttccgta
     1981 ctcggcctgc tggcgttaac tgccctgctc gtcacgaact ctaacccgca atcggtcgcg
     2041 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
     2101 tgggcctgcc ctggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
     2161 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ccactggcaa
     2221 gacaccctgc ccgcagcaga agcctaccgc aatatgtggc tgatgggaat ggcggcgctt
     2281 gccatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
     2341 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
     2401 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg tacgatctgg
     2461 aaggtaacgt ggtttgcgaa ggcaaaggct tattacagcc gatgcacacg ccagatgccg
     2521 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
     2581 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
     2641 gttgctgtcg tgcgttattg aaagccgatg gcacgcctgc tgcgccgttg attagctggc
     2701 aggatgcacg cgttacacgc ccttacgaac acactaatcc tgacgtggca tatgtcacct
     2761 ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat atcgccaact
     2821 attttggtca gtggccggtg gattataaga gctggacatg gagcgaagat gctgcggtaa
     2881 tggataagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcct ggcaccgtcc
     2941 tcggacatat cacaccacaa gccgcactgg cgacacattt cccggcagga ctgccggttg
     3001 tttgtaccac cagtgataaa ccggtagaag ctctgggggc cggattactg gatgatgaaa
     3061 ctgcggtaat ttctcttggc acttacatcg cattgatgat gaacggcaaa gcactgccga
     3121 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
     3181 gttacggtat tcgcaaaggt atgtggacgg tgagctggct gcgtgacatg ttaggcgagt
     3241 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaagaaag
     3301 cctctagtgt gccacctagc tgtaatgggc tgatgacggt gctggactgg ctgaccaatc
     3361 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
     3421 ggatatatcg ttcgatattg gaaagcgtgg cgctaacgct gaagaacaat tacgacaata
     3481 tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggcggc ggttcgaaca
     3541 gcgatctgtt tatgcaaatt tttgccgacg tgttcaacct tccggcacgc cgtaacgcca
     3601 ttaacggttg tgcaagtctg ggagcagcga ttaatacagc ggtaggtctg gggctatacc
     3661 cggattacgc aacggctgtt gataagatgg ttcgcgtgaa agatatcttt ataccgattg
     3721 agagcaatgc caaacgctac gacgcgatga ataaaggcat tttcaaagac ctaaccaaac
     3781 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgtgg
     3841 attcgatcca gagctggtcg aatgcgtaag tgaggaaggc cgggcgggaa actgcccggc
     3901 ctgaacatac ctgaatggtt atccccgctg acgcggggaa cataagttcg acaacttaaa
     3961 aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa aactttaact
     4021 agttcggttt atccctgttt ttacaaggaa tattaactac actcgatgtc tttagaataa
     4081 aagacaatac gaatcgcact ttatcaattt tcacttaaag tatgaactgg atatctttat
     4141 tc
//
LOCUS       DAPPNJ010000284         4133 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000284 DAPPNJ010000000
VERSION     DAPPNJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.286"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(32..2008)
                     /gene="speA"
                     /locus_tag="RPM73_004247"
     CDS             complement(32..2008)
                     /gene="speA"
                     /locus_tag="RPM73_004247"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDX6522517.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="RPM73_004248"
     CDS             complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="RPM73_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDX6522518.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2284..2499
                     /locus_tag="RPM73_004249"
     CDS             2284..2499
                     /locus_tag="RPM73_004249"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522519.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2803..3957
                     /gene="metK"
                     /locus_tag="RPM73_004250"
     CDS             2803..3957
                     /gene="metK"
                     /locus_tag="RPM73_004250"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDX6522520.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1040 a         1128 c         1008 g          957 t
ORIGIN      
        1 ataaatcgcc ccgccagcgg aataatctta attattcatc ttcaagatag gtataaccgt
       61 acaaacctgc ctcgaactct tccaggaact gttgttgcag ttcagcatca agatcggttt
      121 tcttcacttg atcacggaac tgggttaaca acgttttcgg atcgagctgt acatattgca
      181 gcatgtccgc cacggtatcg ccttcgtcag acagttctac ttctacgctg ccgtcaggga
      241 agacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct
      301 cctgatatgc gccgaccata aagaaaccga gcatcggcgg attctctgga tcgtactccg
      361 gcatgggcat tgtcgtggca ataccatcac catcgatgta gtgatcaata gcaccgtcag
      421 agtcacaggt aatatccagc agcacagcgc gacgttccgg cacttgatcc agcccttcca
      481 gcggcagaac cgggaacaac tggtcgatcc cccatgcgtc cggcattgac tggaacagcg
      541 agaaattgac gtacattttg tccgccatac gttcctgcag ttcgtcgatt atcggacgat
      601 gagcacggtt ttgcggatcc agctgcttct gcacttcatg gcacatgctc aaataaagct
      661 gctcagccca tgcgcgttct tgcaggctaa aggtgccgga agagtagccg atatgaatat
      721 cgtgcaaatc catctggctg tcgtgcaacc attcacgcag agaacggcga gttcctggtt
      781 cgtgcatctc ctgccaggtt tcccacatgc tttgcagtgc gcgcggcgca tcttctgcgg
      841 gcgcggtcgg caccgtatat tcgttacgtt ccacgccgat gatattcgac accagcacgg
      901 tatggtgcgc agtcaccgca cggcccgatt cggtgattac cgtcggatgc ggcagaccat
      961 tttcttcaca cgcatcacca attgcccaga tgatgttgtt ggcgtattca ttgaggccat
     1021 agtttacgga gcagtcagac tgcgaacggg tgccttcata gtccacgccc agaccgccgc
     1081 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgccg
     1141 attcacgaac gcctgtggcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
     1201 gcagtagttg caggctgtcg agacgcccgg cttcacgcag ggtttcaacc agttgcagta
     1261 cctgagtcgc agccaggccg aacttcgatt tttccccgcc ggaggactgc catttacccg
     1321 agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
     1381 cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag accttgtgcc
     1441 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtctttataa ccgttgcaga
     1501 cgatgacgct acgggtcatg ccagcatgcg ccagcaccgc catcaactct gctttcgagc
     1561 ctgcttccag ccccaacggt tcgccagaat gaatcaggga ctcaatcacg cggcggtgct
     1621 ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag gattcccgcg
     1681 cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1741 acagtgcagg cagacgctga ccctgtgctt cacgagtttt cactaactgc gcgagatcga
     1801 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1861 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1921 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     1981 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     2041 acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac atggattttc
     2101 cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag
     2161 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     2221 aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct gagaagcgcc
     2281 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc
     2341 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     2401 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac ccggctggaa
     2461 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2521 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt gtcaggaaaa
     2581 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg
     2641 aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2701 ctgccagtgt ttttaacttg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2761 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2821 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2881 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2941 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     3001 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat gggctttgac
     3061 gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat caaccagggc
     3121 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
     3181 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
     3241 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     3301 agccaggtga ctttccagta tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt
     3361 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3421 atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3481 ccgaccggtc gttttgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa
     3541 attatcgttg atacctacgg cggcatggca cgtcacggtg gcggagcatt ctctggtaaa
     3601 gatccatcaa aagtggaccg ttccgctgcg tacgcagcac gttatgttgc aaaaaacatc
     3661 gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3721 gaaccgacct ccatcatggt agaaactttc ggtaccgaga aagtgccttc tgaacaactg
     3781 actctgctgg tacgtgagtt cttcgacctg cgcccatacg gtctgattca gatgctggat
     3841 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3901 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct
     3961 ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     4021 tggataggca ttcacgccgc atccggcaaa aagctgcccg cacaataaca tcattcttcc
     4081 tcaacacgtt ttatcgcaaa ttatcatcac tactgaaacc gattacacca ata
//
LOCUS       DAPPNJ010000285         4082 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000285 DAPPNJ010000000
VERSION     DAPPNJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.287"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            104..307
                     /locus_tag="RPM73_004251"
     CDS             104..307
                     /locus_tag="RPM73_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDX6522521.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            315..1247
                     /gene="aaeA"
                     /locus_tag="RPM73_004252"
     CDS             315..1247
                     /gene="aaeA"
                     /locus_tag="RPM73_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDX6522522.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1253..3220
                     /gene="aaeB"
                     /locus_tag="RPM73_004253"
     CDS             1253..3220
                     /gene="aaeB"
                     /locus_tag="RPM73_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDX6522523.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3312..3584
                     /locus_tag="RPM73_004254"
     CDS             3312..3584
                     /locus_tag="RPM73_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDX6522524.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3640..3903)
                     /gene="yhcN"
                     /locus_tag="RPM73_004255"
     CDS             complement(3640..3903)
                     /gene="yhcN"
                     /locus_tag="RPM73_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDX6522525.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          949 a          985 c         1081 g         1067 t
ORIGIN      
        1 aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg cgacagatgc
       61 tgaaaataat aacgcctgct ctctctatac aaccaaggtc gacatgagtc tgtttcccgt
      121 tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt tatcactggc
      181 gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact ttgtctggca
      241 tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat cgcgactgtt
      301 cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc atcacggtcg
      361 tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac accgaatccc
      421 cgtggacgcg tgacgcgcgc tttagcgctg atgtcgttgc gatcgcgccg gacgtttctg
      481 gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga caggtactgt
      541 tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt
      601 attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaat cgtctcggtg
      661 tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa acggttctgc
      721 atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt gaacgcacgg
      781 tgatccgcgc gccagcggat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta
      841 ttactcgtgg atcaacggcg gttgcgctgg tgaaacagaa ctctttctat gtactggcct
      901 atatggaaga aactaagctg gaaggggtgc gccctgggta tcgtgcagag atcacgccgc
      961 ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgctggg gtcaccaacg
     1021 ccagcagcac gcgtgacgac aaagggatgg cgaccatcga ctctaacctt gaatgggtgc
     1081 gtttggcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc
     1141 ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg
     1201 attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat
     1261 tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact
     1321 ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc
     1381 ggcgattgtt gctgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat
     1441 tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt
     1501 gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc
     1561 cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtatg cgtgggggct
     1621 ggccggttat accgcgctga tcattgtgat caccattcag cctgaaccat tgcttacgcc
     1681 gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg caattatggc
     1741 ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc tggaaagttt
     1801 gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt
     1861 cgataaagcc tggggcgacc tggttcgacg caccacggcg ctacaaggta tgcgcagcaa
     1921 cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac
     1981 gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc
     2041 ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga
     2101 cgtccacaag cagctcaaac gcctgcgaag agttatcgcc tggaccgggg aacgggaaac
     2161 gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg
     2221 cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga
     2281 agtcaaagta gagtcagccg aacgtcatca tgcgatggtt aacttctggc gaaccacact
     2341 ttcctgcatt ctgggaacgc ttttctggct gtggacgggc tggacttccg gcagtggtgc
     2401 aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt
     2461 ggcgatcgac tttatctacg ggacgctggc cgcgctgccg ttagggctgc tctacttttt
     2521 ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc tggcagtgct
     2581 gggattcttc ctcggaatag aagtacagaa acggcgactg ggctcgatgg gggcgctggc
     2641 cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct
     2701 cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt
     2761 gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc
     2821 tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta
     2881 tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc
     2941 gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga
     3001 tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag
     3061 cgatgataaa cgtcgtcggt actttggtca gttgctggaa gaactggaaa tctaccagga
     3121 aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc ggctggcggg
     3181 gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag
     3241 cgtcggtttt ttcatggcta tacttagcga tgaacggcag aactcgccgc gaaacgtgac
     3301 ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt caggaggatt
     3361 tttatcgcga cttcagccaa gcctttggtc tggcgaaaga taaggtacgc gatctcgact
     3421 cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc
     3481 atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag
     3541 aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc
     3601 cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca gttcagccgt
     3661 agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc
     3721 ttccgctttt ttgttcagca tttcacgtat atccattggc gaagacgcca caccacttac
     3781 ggatacggtc ccgattgctt cacggttttg tgcttgtgca gcatcaatgg agtcggcagc
     3841 gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gcaacagtgg ttttgatttt
     3901 catgattttt acctcgacat aatctttttg ctgggtcttt gtttcgtgac ccttatcaca
     3961 aaatcaagta tacactaatt attgaactaa ttaataccac gctaactgtt tctgttgata
     4021 ctaagtgtta aaaatgtgaa tgttaataac aaaaaagaat taaaaatacg gttatttatc
     4081 gt
//
LOCUS       DAPPNJ010000286         4053 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000286 DAPPNJ010000000
VERSION     DAPPNJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.288"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..717
                     /gene="yibB"
                     /locus_tag="RPM73_004256"
     CDS             <1..717
                     /gene="yibB"
                     /locus_tag="RPM73_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HDX6522526.1"
                     /translation="NEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEK
                     IQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGY
                     CRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGS
                     QHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRC
                     FRSNTLGAKMQALRIFLSRK"
     gene            992..2188
                     /gene="kbl"
                     /locus_tag="RPM73_004257"
     CDS             992..2188
                     /gene="kbl"
                     /locus_tag="RPM73_004257"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDX6522527.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2198..3223
                     /gene="tdh"
                     /locus_tag="RPM73_004258"
     CDS             2198..3223
                     /gene="tdh"
                     /locus_tag="RPM73_004258"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDX6522528.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3462..>4053
                     /gene="waaH"
                     /locus_tag="RPM73_004259"
     CDS             3462..>4053
                     /gene="waaH"
                     /locus_tag="RPM73_004259"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDX6522529.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTE"
BASE COUNT         1027 a          882 c         1048 g         1096 t
ORIGIN      
        1 aatgagatga ttattttcac ttctcccgat ttgaaaccaa gggttgaagc gattcgcaac
       61 ggaaaaccaa caacggttat cgttatcgat ataaaaaaga aatttagata tatcagaagt
      121 cgtatcgaaa aaattcaaaa agatgagtcg tttacaaata gacttgagcc tcgacaatta
      181 aaaaacccag agtactggtc accagagtat gttttggtat gtaaccttaa ggcctatttt
      241 gtaaataaag ctatcaacat gggcttagtg aaaacgcctc ttgttgcgtg gatagatttt
      301 ggctattgcc gtaagccaaa tgtaactcga ggattgaaaa tttgggattt cccatttgat
      361 gaaagtaaga tgcatctttt cactataaaa aaagggctta ctgtcacatc tcaacaacag
      421 gtttttgatt tcatgattgg caatcatgta tatattattg ggggcgcaat tgttggttca
      481 cagcataaat ggaaagaatt ctataaatta gttctggaaa gtcaaaaaat aacactaaat
      541 aataacattg tggatgatga ccagggcatt tttgtcatgt gctattacaa acgccccgat
      601 ctcttcaacc taaactatct ggggagagga aaatggttcg atttgtttcg ctgcttcagg
      661 agtaacactt taggggcaaa aatgcaggcg ctgagaattt tcctttcaag aaaataggtt
      721 tccctccatt agtgggtagc gttttgatac caactgagat aggaaaaatg atgctttggc
      781 atcatttttt ctttttatat caatgcagta aaatcaatca tcattatgcc cagccaaaac
      841 tgtacagttt ttctactatc tgtaggtgtt gagagacatc aagttacacg ttatttatcc
      901 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
      961 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
     1021 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
     1081 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
     1141 caactatctc gggctggcga atcatcctga tctgattgcg gcggcaaagg cgggaatgga
     1201 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
     1261 agagcttgaa caaaaactgg cggccttcct ggggatggaa gatgcgattc tctactcttc
     1321 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
     1381 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
     1441 ctatcgctat gccaacaacg atatgcagga gcttgaagct cgtctgaaag aagcgcgtga
     1501 agccggtgcg cgtcatgtgc tgattgccac cgatggtgta ttctcgatgg acggcgtgat
     1561 tgccaatctg aagggcgttt gcgatctggc agataaatac gatgccctgg tgatggtaga
     1621 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
     1681 tgtgatgggc cgggtcgata ttatcaccgg tacgcttggt aaagcgctgg gcggggcttc
     1741 tggtggttat accgcggcgc gcaaagaggt ggttgagtgg ctgcgccagc gttctcgtcc
     1801 gtacctgttc tccaactcgc tggcaccggc cattgttgcc gcgtccatca aagtactgga
     1861 gatggtcgaa gcgggcagcg aactgcgtga ccgtctgtgg gcgaacgcgc gccagttccg
     1921 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcatgcca ttattccggt
     1981 catgcttggc gatgcagtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
     2041 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
     2101 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
     2161 tggtaaacaa ctgggcgtta tcgcctgagg atgtgagatg aaagcgttat ccaaactgaa
     2221 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
     2281 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
     2341 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgta ggccatgaat atgtcggtga
     2401 agtggtaggt attggtcagg aagtgaaagg cttcaaaatc ggtgatcgcg tttctggcga
     2461 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
     2521 caacaccatt ggtgtcggtg ttaaccgtcc tggctgcttc gccgaatatc tggtgattcc
     2581 ggcgttcaac gctttcaaaa tccccgacaa tatctccgac gacctggctt ccatttttga
     2641 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggtgggcg aagatgtgct
     2701 ggtttctggt gcaggtccga taggtattat ggctgcggcg gtggcgaaac acgttggtgc
     2761 acgcaatgtg gtgatcaccg atgttaacga ataccgcctt gagctggcgc gcaaaatggg
     2821 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
     2881 catgaccgaa ggctttgatg tcggtctgga aatgtccggc gcgccgccag cgtttcgtac
     2941 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
     3001 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
     3061 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
     3121 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
     3181 gcgttcgggc cagtccggga aagtaattct gagttgggat taacacgaac aagggctggt
     3241 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
     3301 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
     3361 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
     3421 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
     3481 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
     3541 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
     3601 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
     3661 atcaggcgaa tgctggcgca tcggtagcgc gtaatcgtgg gatcgaggtg gcgacgggca
     3721 aatatgtcgc ttttgtcgat gctgacgatg aggtctatcc caccatgtac gaaacgctga
     3781 tgaccatggc gttagaggat gacctcgacg tggcgcagtg caacgctgac tggtgttttc
     3841 gtgaaacggg agaaacctgg caatccatcc ccagcgatcg ccttcgctca accggcgtat
     3901 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
     3961 ggatgggggt ttatcgccgt gatgttattg ttaaaaataa cattaaattt attgccggat
     4021 tacatcatca ggatattgtc tggacaacag aat
//
LOCUS       DAPPNJ010000287         4050 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000287 DAPPNJ010000000
VERSION     DAPPNJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.289"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1238
                     /gene="frc"
                     /locus_tag="RPM73_004260"
     CDS             <1..1238
                     /gene="frc"
                     /locus_tag="RPM73_004260"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDX6522530.1"
                     /translation="QGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQL
                     RDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGF
                     TWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAA
                     ALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGY
                     LEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTC
                     KAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVL
                     SMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVL
                     QELGYSDDEIAAMKQNHAI"
     gene            1292..2986
                     /gene="oxc"
                     /locus_tag="RPM73_004261"
     CDS             1292..2986
                     /gene="oxc"
                     /locus_tag="RPM73_004261"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDX6522531.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3056..4000
                     /gene="yfdV"
                     /locus_tag="RPM73_004262"
     CDS             3056..4000
                     /gene="yfdV"
                     /locus_tag="RPM73_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDX6522532.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
BASE COUNT         1041 a          876 c          986 g         1147 t
ORIGIN      
        1 ttcaaggaat taaagttctc gatttcaccg gtgtgcaatc tggcccatct tgtactcaaa
       61 tgctggcctg gtttggcgct gacgttatta aaattgaacg tcccggcgtt ggtgacgtaa
      121 cgcgtcacca gctgcgagat attcctgata tcgatgcgct ttacttcacc atgcttaaca
      181 gtaacaaacg ttctattgag ttaaatacca aaacagcgga aggcaaagag gtaatggaaa
      241 agctgatccg cgaagctgat atcttagtcg agaactttca tccaggggcc attgatcaca
      301 tgggcttcac ctgggagcat attcaagaaa tcaatccacg tctgattttt ggttcgatca
      361 aagggtttga tgagtgttcg ccttatgtga atgtaaaagc ctatgaaaac gttgctcagg
      421 cagcgggtgg cgcggcatcc actacgggtt tttgggatgg tccgccgctg gtaagcgctg
      481 cagcgttggg tgacagcaac accggaatgc atttgctgat cggtttactt gctgctttgc
      541 tgcatcgcga aaaaacgggg cgtgggcaac gagtcaccat gtcaatgcag gatgccgtat
      601 tgaacctttg ccgcgtgaaa ttacgtgacc agcagcgtct cgataaattg ggttatctgg
      661 aagaataccc gcagtatccg aatggtacat ttggtgatgc agttccccgc ggtggtaatg
      721 caggtggtgg cggtcagcct ggctggatcc tgaaatgtaa aggctgggaa accgatccta
      781 acgcctatat ttatttcact attcaggagc aaaactggga aaacacctgt aaagccatcg
      841 gcaaaccaga atggattacc gatccggcat acagtacagc ccatgcacga cagccacata
      901 ttttcgatat ttttgctgaa atcgaaaaat acactgtcac tattgataaa catgaagcgg
      961 tggcctattt gactcagttt gatattcctt gtgcaccggt tttaagtatg aaagaaattt
     1021 cacttgatcc ctctttgcgc caaagtggca gtgttgttga agtggaacaa ccgttgcgtg
     1081 gaaaatatct gaccgttggt tgtccaatga aattctctgc ctttacgccg gatattaaag
     1141 ctgcgccgct attaggtgaa cataccgctg ctgtattgca ggagctgggt tatagcgacg
     1201 atgaaattgc tgcaatgaag caaaaccacg ccatctgata ttaacggggg cttctggctc
     1261 ccaatttata aaaatttcga ggttattaat catgtcagat caacttcaaa tgacagatgg
     1321 tatgcatatc atcgttgaag cattaaaaca gaataatatt gacactattt atggtgttgt
     1381 aggtattcct gtgacggata tggcacgcca tgcccaggcg gaaggcattc gttatattgg
     1441 ttttcgtcat gagcagtcgg caggctatgc cgctgcggca agcggttttc ttacccaaaa
     1501 accggggatc tgcctgacag tttctgcgcc aggattcctc aatggtttga ccgcattggc
     1561 caacgcaacg gtaaatggtt ttccgatgat catgattagc ggctccagcg accgcgcgat
     1621 cgtcgaccta cagcaaggtg attatgaaga gctggaccaa atgaatgcgg caaaaccgta
     1681 tgccaaagca gcatttcgcg ttaatcagcc gcaggatctt ggcattgcat tggcacgcgc
     1741 tatccgggtc tctgtatcgg gtcgccctgg cggagtttat cttgatttgc cagcaaatgt
     1801 cctggccgcg acgatggaaa aagacgaagc gttaaccacg attgttaaag ttgaaaatcc
     1861 gtcgccagca ttattgccat gcccgaagtc agtcactagc gcaatttcgc ttttagcaaa
     1921 agctgaacgg ccattaatta tccttggcaa aggcgcggcg tattcacaag ctgatgaaca
     1981 gcttcgtgaa tttattgaaa gtactcagat tccattcctg ccaatgtcta tggcgaaagg
     2041 gatccttgaa gatacgcatc cactttctgc ggcagctgcg cgttcgtttg ccctggcaaa
     2101 tgctgacgtt gtcatgcttg ttggtgcacg actgaattgg ttattggcac acggtaaaaa
     2161 aggatgggcg gcagatacac agtttattca actggatatt gaaccgcagg aaattgacag
     2221 caaccgcccc attgctgtgc cagtcgttgg tgatattgca tccagtatgc aaggtatgct
     2281 ggcagaactg aaacaaaaca catttacgac tccactggta tggcgcgata ttttaaatat
     2341 ccacaagcag caaaatgcac aaaaaatgca tgaaaaatta agtacagata ctcaaccatt
     2401 aaattacttt aatgcattaa gtgctgtgcg cgacgtattg cgcgagaacc aggatattta
     2461 tttagttaat gaaggtgcaa ataccctgga taatgcacga aatattattg atatgtataa
     2521 accacgtcgt cgtctggatt gtggtacctg gggtgtcatg ggcatcggta tgggctatgc
     2581 catcggtgct agcgtgactt ctggttctcc ggttgtcgcc attgaaggtg atagtgcttt
     2641 tggtttcagt gggatggaaa ttgaaacgat ttgtcgatat aacctgccgg tgacgatcgt
     2701 tatttttaat aatggcggca tctacagagg agacggtgtt gatctcagtg gcgctggtgc
     2761 accatcacca acggatctgt tgcaccatgc aaggtatgac aaattaatgg atgcgtttcg
     2821 tggcgttggc tataacgtca ccacgacaga tgaacttcgt catgctttaa ccaccggtat
     2881 tcagtcgcgc aaaccgacca ttattaatgt ggtcatcgac cctgcagcag gaactgaaag
     2941 tggccatatt accaaactta acccaaaaca agtcgctggt aattaaatac tgacatcgtc
     3001 tcctcttgcc aactcattat gcgatggtga ggggaccctt atcggaaata ctattatgct
     3061 aacatttttt attggcgatt tattgcctat tatcgtaatc atgctgttgg gttattttag
     3121 cggcagacga gaaacttttt cagaagatca agcccgcgca tttaataaac tggtattaaa
     3181 ctacgctctt cctgcggctc tatttgtttc tattacccgg gcaaacaggg aaatgatttt
     3241 tgcggacact cgtctgaccc ttgtatcact tgtggttatt gtcggatgtt tctttttctc
     3301 ctggtttggt tgctacaaat tttttaaacg tacccatgca gaagcagctg tatgtgcatt
     3361 aattgcgggt tcacctacca ttggattcct ggggtttgca gttctcgatc ctatttatgg
     3421 tgattccgta tcaacaggtt tagtggtagc aattatttct attattgtta acgcaattac
     3481 tattcctatt ggtctgtatt tgctgaatcc ttcctcagga gcggatggta agaagaatag
     3541 taatctgagc gcattaattt ctgcggcaaa ggagccagta gtatgggcac ctgttctggc
     3601 aacgatcctg gtgttggttg gggtaaaaat tccggcagca tgggacccaa cctttaatct
     3661 gattgcgaag gctaactcag gggtagcggt attcgctgcg gggttgactc tggctgcaca
     3721 taaattcgag ttcagtgccg aaattgctta taacaccttc ctgaagctga ttctgatgcc
     3781 actggcactg cttctcgttg gtatggcatg tcatttgaac agcgaacatc tgcagatgat
     3841 ggtactggca ggcgcattac cgccggcatt ctccggaatc attattgcca gccggtttaa
     3901 tgtctacacc cgtactggta cagcgtcatt ggctgtgagc gtactgggct ttgtcgtcac
     3961 ggctcccttg tggatttatg tcagtcgact ggtttcataa ttaatcatgt tattgatttt
     4021 acataaatgc cgaagtgtct tttcaccttc
//
LOCUS       DAPPNJ010000288         4022 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000288 DAPPNJ010000000
VERSION     DAPPNJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.290"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(3..>4022)
                     /gene="rhsD"
                     /locus_tag="RPM73_004263"
     CDS             complement(3..>4022)
                     /gene="rhsD"
                     /locus_tag="RPM73_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDX6522533.1"
                     /translation="IFGPGWKAPSDIRLQIRDDALVLNDNGGRSIHFEPLLPGEAVYS
                     RSESLWLVRGGKATQPDGHTLARLWASLPPDIRLSPHLYLATNSAQGPWWILGWSERV
                     PGAEDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLT
                     TQAQRAEEARKQRTASLSSPDTPRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPA
                     YPESLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRY
                     RYDDTGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELAD
                     GSVTRSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLT
                     AVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTW
                     SRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRE
                     TRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNE
                     NGSHSVFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITH
                     RTVNGDPAEQWQYDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGEL
                     LWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRE
                     TVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQT
                     REYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAKD
                     AHYVYHYDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSLHRLVHYIRIQYEEPLVES
                     RYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVY
                     QPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEI
                     RADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKAHLYHCDHRGLPLALISEDGNT
                     AWSAEYDEWGNQLNEENPHHVYQPYRLPGQQHDEESGLYYNRHRYYDPLQGRYITQDP
                     MGLKGGWNLYQYPLNPLQQIDPMGLLQTWDDARSGACTGGVCGVLSRIIGPSKFDSTA
                     DAALDALKETQNRSLCNDMEYSGIVCKDTNGKYFASKAETDNLRKESYPLKRKCPTGT
                     DRVAAYHTHGADSHGDYVDEFFSSSDKNLVRSKDNNLEAFYLATPDGRFEALNNKGEY
                     IFIRNSVPGLSSVCIPYHD"
BASE COUNT          681 a         1317 c         1091 g          933 t
ORIGIN      
        1 aattaatcat gatacggtat gcatactgaa ctcaatcccg ggacactatt tctgataaaa
       61 atatattctc ctttattatt aagcgcctca aatcgtccat caggtgttgc gagataaaat
      121 gcttcaagat tattatcttt acttcttaca agatttttat cgctacttga aaaaaattca
      181 tcaacataat cgccatgact atctgcaccg tgagtatgat aagcagcaac tctatctgta
      241 cctgtgggac attttctttt cagaggatat gactcctttc ttaaattatc agtttctgcc
      301 ttagatgcaa aatattttcc attagtatct ttacagacaa taccagagta ttccatatca
      361 ttacatagag atctattctg cgtttctttc aaagcatcta acgcagcatc tgcagtacta
      421 tcaaatttac ttggtcctat tatacgtgaa agaacaccac aaactccccc cgtacatgct
      481 ccagatctgg catcatccca agtctgcaat aatcccatag ggtcaatttg ttgtagtgga
      541 tttaaaggat actgatataa attccatccc cctttcaacc ccatcgggtc ctgagtaata
      601 taccgcccct gcaacggatc gtagtaccgg tgacggttat agtacagccc tgattcctca
      661 tcatgctgct gccctggcag acggtacggc tgatacacat gatgcgggtt ctcctcatta
      721 agctggttgc cccattcatc atattccgcg ctccacgccg tattgccgtc ttcgctgata
      781 agcgccagcg gcagtccccg gtggtcgcag tgataaagat gagcttttcg cgccggtgtg
      841 tattccggct ccacctgtct ggccagttgc tccaccgtca gcccgcactg cgcaagccac
      901 gcccggcttt cactgctcac gcggtctgcc cggatttctt cctccagtct gtccagcagc
      961 cgcaccagtt cagccggaaa taccacgccg tgcccgtcct cgctcccttc ctgctgcagc
     1021 ttctccgcca ggctgcggcg ctgcgctttc tcccgctcgc cgttgtctgt ttcgatgcgg
     1081 atgagcggcg cgaagctccc cggctgatat accgtctgga tacgcgtggt gtcggtctgt
     1141 accgtcgtca gccggtcgcc gtcccacccg taccacgtca cctccggttt ccgcgacagc
     1201 gacatccatc ccgtcaggtc acgctcgcga cgccacaccc gtttccctgt ccggcgaccc
     1261 agcgggtcgt aaaggtatcg gctctcgacc agcggctcct catattggat acggatgtaa
     1321 tggaccaggc ggtgcaggct gtcgtaatgg tagtgatggg tgcgctcgtc gtccgtccgt
     1381 atcacacccg ccgggatgcg gtccgtcttc tccgtcagtc tgccgtattc atcgtagtgg
     1441 taaacatagt gcgcatcctt cgcgatgcgg ttatccggcc acactgtgag ggtactgtcc
     1501 gggtgcagct ccgggtccgg cagccggttg cccgccgggt ccgtggcata cgggatgcgg
     1561 atgtccaggt ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg
     1621 tattcccgcg tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg
     1681 tagtcacggt catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg
     1741 tatgtgctgg tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc
     1801 gtctcccggt gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg
     1861 cccgccaggt aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg
     1921 acgcggtttg ccagcccctg ttcgttgtat gcgtgtttcg tctcatgctg ccacagcagt
     1981 tcccccgtct ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg
     2041 tagccgtagt ggacagcgac acggtggcct tcgctcaggt ggctgatttc tctcagccag
     2101 ccgtggccgt catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg
     2161 cggtccgacg catcgtagtg ccagagggtg acaagcccct catcttcact ctgcgtgagt
     2221 tttccggtca ggtcataatg ataacgttgc gtccgcccgt caaagccgcc ctgctgtacc
     2281 agccggtcca gcgcatcgta actgaagacg ctgtggctgc cgttctcgtt ggtcaggctg
     2341 atgacacgtc cggcagcatc gtactccata ctgcgcgtca gcccgccctg cgtggtgctg
     2401 accgcctttc cccacgcatc gtactgtgtc tcgctccggt tgccgtccgg ggtgataacg
     2461 gcagtcaggt cgcctgcggc gttgtattca taccgcgttt cacggccctg tgcgtctttc
     2521 accgaggtta accggccacg gttgtcatag cggcggtaaa ggctgatgcc ttcctcgcgg
     2581 tggaccgccg tcatctggcc gaagcggtcg tattcataac gggtctggta gcccgagcag
     2641 tcggtgaacg ccagcaactg cccgtagcgg ctccaggtca tctgccgggt gctgcccgtc
     2701 gcatctgttg tcgtggccgg taactcactg tgcgcgtcat cgtagcggta gcgtactgtc
     2761 tccccgctgc gcgatgtctc cgataccagc ctgcccggtt catcatattc ccggcggctc
     2821 tccagcccgt ccggggacac caccgccgtc agctggttcc cgtcgttata gtaaaatttc
     2881 gtctcccgcc cgtccggtgt ggtgatgtcc gtgatatcgc cggacaccac attcagaccg
     2941 tactctgtcc gccgtcccgc cgcgtccgtc tgcgccgtga gccttcctgc cgcgtcatac
     3001 ccgctgcgcg tgacgctgcc gtccgccagt tcttttttca ccacccgttt cagcccggcc
     3061 ccgccctctg tatgcagcac ctcacgccgg ttcaggctgt cggtgacggt gatgcggtcc
     3121 tgctcataaa gatagcggta gcttaaccct gccgggttca gttgctccac cacccgcccc
     3181 gtatcgtcgt agcggtagcg catctccggc cttcccgcgt aacggtgcgc caccatccgg
     3241 cccgggtgct gcgcgtcata cgtgaaagcg cgcacctgcg tattgctgcg gtcatatacc
     3301 gccagcagtt caccggcttc cgtatacgtg taccgcgcca gtggcgcacc gggcaggctt
     3361 tccgggtatg ccgggtcgtg catcagccac accgccgaaa gacggatgcc cctgtcgggg
     3421 ccgtattcgg taccgggcag tgtgtcgggg aacgctgagg ctgagagagg gcggggtgtg
     3481 tcaggggaag ataaggaagc ggtgcgctgt ttacgggcct cttccgcacg ctgcgcctgc
     3541 gtggtcagca ccagacggaa ctcccgcccg gcaccgtccg tcacgccggt gatttccccg
     3601 gccaggtcac cggcggcctc acgccggtac gtcagcgtcc gcccgaagcg gtctgccagc
     3661 ccggtaagca cccggtacgg cggcagcggc gctggcagta cgtcctcagc acccggcacc
     3721 cgctctgacc accccagtat ccaccacggc ccctgtgcgc tgttggtcgc caggtaaaga
     3781 tgcgggctta accggatatc cggcggcagc gacgcccaca gccgtgccag cgtatggccg
     3841 tccggctgcg ttgccttacc gccacgcacc agccacaggg actcgctgcg gctgtacacc
     3901 gcctcacccg gcagcagcgg ctcaaagtga atgctccgtc cgccgttgtc gttgagtacc
     3961 agtgcatcat cgcgtatctg taagcggata tcagaaggcg ctttccagcc ggggccaaaa
     4021 at
//
LOCUS       DAPPNJ010000289         3966 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000289 DAPPNJ010000000
VERSION     DAPPNJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.291"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            74..682
                     /locus_tag="RPM73_004264"
     CDS             74..682
                     /locus_tag="RPM73_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDX6522534.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            780..2171
                     /gene="selA"
                     /locus_tag="RPM73_004265"
     CDS             780..2171
                     /gene="selA"
                     /locus_tag="RPM73_004265"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDX6522535.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2168..>3966
                     /gene="selB"
                     /locus_tag="RPM73_004266"
     CDS             2168..>3966
                     /gene="selB"
                     /locus_tag="RPM73_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDX6522536.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT          867 a         1020 c         1191 g          888 t
ORIGIN      
        1 accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt tttcctcgca
       61 caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac gcaaactttc
      121 tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc cctataacgc
      181 ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg tgaccgaaga
      241 gggcgaatgt tggtttgatt cgccgatcat cgctgaatat attgaattaa tgaatgtcgc
      301 tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa ttgaggcact
      361 ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc gtccagcggc
      421 gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca gtctggatgt
      481 gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc tggcgactat
      541 cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc gttgcgccag gctggtgtgt
      601 cgatcgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa gttttgctcg
      661 caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc acgttacaat
      721 ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc aggtttccta
      781 tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc ttattgcgcg
      841 atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg gaattgttgc
      901 gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg cctgcgtggt
      961 gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaagcgcag agcgcgctgc
     1021 gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga gctttacagg
     1081 cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc ctcgagtacg
     1141 atctggacga cgctggacgc ggacatcgcg atcgtgcgct ggcgcagttg ctgtgccgca
     1201 ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg ttattgatgt
     1261 tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaactg gtggagattg
     1321 gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta cacgaagtag
     1381 ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa aataccgcac
     1441 tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa gcgatagatg
     1501 aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact gatttaggca
     1561 gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg ccgcaggagt
     1621 tgattgcggc gggcgtcagt ctggtgagtt tctccggcga caagttgtta ggcgggccgc
     1681 aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc cacccgctga
     1741 agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg ttgcgtcttt
     1801 atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt acccgcagcg
     1861 cagaggtcat tcaaatccag gcacaacgtt tacaggcccc ccttgccgca cattacggcg
     1921 cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt tcgctgccgg
     1981 ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc ggtagccacc
     2041 ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt cgtatttatg
     2101 acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt ttggagatgt
     2161 tgttgaaatg attattgcga ctgccggaca cgttgaccat ggcaaaacaa ccttattgca
     2221 ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg gcatgaccat
     2281 cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctggtt ttatcgacgt
     2341 acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta tcgatcacgc
     2401 gctgttggtg gtggcgtgcg atgacggcgt gatggcacag acccgtgagc atctggcgat
     2461 tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg atcgcgtgga
     2521 cgaagcgcgt gttaatgagg ttgaacgcca ggtaaaggag gtgctgcggg aatacggttt
     2581 tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg atgccctgcg
     2641 cgagcatctg cttcagttgc cggaacgcga gcacgccagc caacatagtt tccgcctcgc
     2701 tattgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta cggcgttaag
     2761 cggggaagtg aaggtaggcg attcactctg gctgactggt gtaaataaac cgatgcgtgt
     2821 acgtgcgctg catgcgcaaa accagccaac agaaaccgcc catgccgggc agcgtatcgc
     2881 gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact ggctgcttgc
     2941 cgatgtgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc atacaccgct
     3001 gacccagtgg cagccgctgc atattcacca cgccgccagc cacgtcacgg gacgcgtttc
     3061 actgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc tggcggataa
     3121 cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg cgcgtgtggt
     3181 gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat ggctggcgtc
     3241 tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac gcggcgcggt
     3301 taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc gcgaattgct
     3361 gcaacagcct ggctatattc aggctggtta tagcttgttg aatgcgccgg ttgccgctcg
     3421 ctggcaacgg aaaattctcg acacattagc gacttatcat gagcaacatc gcgatgaacc
     3481 tgggcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg aagcgctagt
     3541 actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc acggttggct
     3601 gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct ggcaaaaagc
     3661 agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag agacgggtac
     3721 cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa taattaccgc
     3781 gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca atatgatccg
     3841 cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc gcttaggcgt
     3901 aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct ttacgcgtcg
     3961 tcgtgg
//
LOCUS       DAPPNJ010000290         3942 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000290 DAPPNJ010000000
VERSION     DAPPNJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.292"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(4..168)
                     /locus_tag="RPM73_004267"
                     /pseudo
     CDS             complement(4..168)
                     /locus_tag="RPM73_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            235..762
                     /locus_tag="RPM73_004268"
                     /pseudo
     CDS             235..762
                     /locus_tag="RPM73_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            759..1745
                     /locus_tag="RPM73_004269"
     CDS             759..1745
                     /locus_tag="RPM73_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522537.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1938..2025)
                     /locus_tag="RPM73_004270"
     tRNA            complement(1938..2025)
                     /locus_tag="RPM73_004270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1989..1991),aa:Ser,seq:gga)
     gene            2259..3197
                     /gene="ghrA"
                     /locus_tag="RPM73_004271"
     CDS             2259..3197
                     /gene="ghrA"
                     /locus_tag="RPM73_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDX6522538.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3252..>3942
                     /gene="ycdX"
                     /locus_tag="RPM73_004272"
     CDS             3252..>3942
                     /gene="ycdX"
                     /locus_tag="RPM73_004272"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDX6522539.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNF"
BASE COUNT          957 a          854 c          989 g         1142 t
ORIGIN      
        1 ctaattttgc tgtttactgc gccagttgta gcgctgtgat tcatacaggc tgagttcacg
       61 ggctgcggca gtaacaccga tgcgttcagc aagcttcagg gcttcactgc gaaattcagg
      121 cgaatgctgt ttacggggtt ttttactggt tgatactgtt tttgtcatgt gagtcacctc
      181 tgactgagag tttactcact tagccgcgtg tccactattg ctgggtaaga tcaggcggtt
      241 aaaggcatca accgcgaaca actgcagacg atgattacgg aatcaggcat tgcgctggat
      301 attagctgtg ataacacgcc gcgccagcag gttattgggg gaacgcaggc tgcgttgaat
      361 gaatttgcca cgctgctgat ggccgctgga tacgaaccgg ttaaactcgg tgtgagcggt
      421 gcctggcaca cgcgtttgat ggaagacggc gttcaggcaa tgcgcgatta tcttgccgga
      481 ctggatatcg cttcgccaga gcatcaggtt ctgatgaacg taacagcaaa aagcgaggtc
      541 gctccgtcaa ttatcaaaga aaatctctca ttacacttaa ctcatacggt gaaatggact
      601 gaatctttcg atacatttct gaatatgcca accccggtag ctttccttga aataagtaat
      661 aagccctatt tggggaatat gttaaatgat tttgctggcg tggatcagca acgagttatg
      721 cattgtcgta aagcatttag tgatgcaaag gtattcaaat gattcctgat tatttaactt
      781 ttattcgctt tcaggataaa cgaaatctga tatacattta tgctattgga cttattctga
      841 taggctttta ttggaagaat gcagggttta cttttccatc agaggatatt ggtgtagtta
      901 gtgggattct ggccctggtg ctgtataatt ttatttttga tctcaaggcg tactgggctt
      961 ataaatgcgt cacgaagaat atcgattttt cgtggtttaa gaaaaagcag aaccacaaaa
     1021 tagaattatt tcttacacaa cctctggtgg caggatttct gtcgttaatc atgttgagtg
     1081 caatgagttg ggggctatac cagcttctac cctcgttata tgcgctgttc ctgatttcgt
     1141 tacttgggcc gttggtcatc tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc
     1201 aggtcgctat ttcagtagcg aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc
     1261 tttcggtgtg catctcaacg gttgttaacc tgcttactat cagcccgttg cgtaacagtg
     1321 attcttttgt gacagagggg cagtggttaa cgtttaaatc gataattgca ttgctcattc
     1381 tttgtggcgt agtgttggcg attaatctgt tttttctgcg cttctccaag cggtacgctt
     1441 ttctgggcag gctttttttg caggaaatcg atctgttttt ctccagtgaa aatgcgttgt
     1501 cgaccttttt tgccaagccg ctttggcttc ggttattcat attgctggtt attgaaatga
     1561 tgtggattac gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag
     1621 cctatttttt actctgctat gtaccgtgct taatttacta ttttttccat tgtcgattcc
     1681 tctggcataa cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata
     1741 agtgaggaag ttacaggaag tgaccagata acgcatatat gttctctact atcttgcgca
     1801 ttttgattgt tgactgagta accagacagt tgatgtgcac aatttcccct cgccctaaca
     1861 gacgtgggcg ggggcacccg gtgtgtgcaa tatacgaaaa aaaagcccgt actttcgtac
     1921 gggctcttct ttaaatatgg cggtgagggg gggattcgaa cccccgatac gttgccgtat
     1981 acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gtcgttcctg
     2041 tcttgctgga acgggcgcta atttagggaa atcatgacct gaggtcaaca aactttttga
     2101 aaaaatcgcg cgtttattca aacttcaatc aatgtgtggt tttagtaagc gaaatctgct
     2161 ttttttgcca ccgaccacgg atttgttatg ctggtggcct ttgtagatca taacgataag
     2221 tgcgaataaa tttcgcacaa cgcttttcgg gagtcagtat ggatatcatc ttttatcacc
     2281 caacgttcga tacccaatgg tggattgagg cactgcgcaa agctattcct caggcaagag
     2341 tcagagcgtg gaaaagcgga gataatgact ctgctgatta tgctttagtc tggcatcctc
     2401 ctgttgaaat gctggcaggg cgcgatctta aagcggtgtt cgcactcggg gccggtgttg
     2461 attctatttt gagcaagcta caggcacacc ctgaaatgct gaacccttct gttccacttt
     2521 ttcgcctgga agataccggt atgggcgagc aaatgcagga atatgctgtc agtcaggtgc
     2581 tgcattggtt tcgacgtttt gacgattatc gcatccagca aaatagttcg cattggcaac
     2641 cgctgccgga atatcatcgg gaagatttta ccatcggcat tttgggcgca ggcgtactgg
     2701 gcagtaaagt tgctcagagt ctgcaaacct ggcgctttcc gctgcgttgc tggagtcgaa
     2761 cccgtaaatc gtggcctggc gtgcaaagct ttgccggacg ggaagaactg tctgcatttc
     2821 tgagccaatg tcgggtattg attaatttgt taccgaatac ccctgaaacc gtcggcatta
     2881 ttaatcaaca attactcgaa aaattaccgg atggcgcgta tctcctcaac ctggcgcgtg
     2941 gtgttcatgt tgtggaagat gacctgctcg cggcgctgga tagcggcaaa gttaaaggcg
     3001 caatgttgga tgtttttaat cgtgaaccct taccgcctga aagtccgctc tggcaacatc
     3061 cacgcgtgac gataacacca catgtcgccg cgattacccg tcccgctgaa gctgtggagt
     3121 acatttctcg caccattgcc cagctcgaaa aaggggagag ggtctgcggg caagtcgacc
     3181 gcgcacgcgg ctactaataa agcatcagga ttcctgctat ccttggcggg aattgaatac
     3241 aggagagagt tatgtatccc gtcgaccttc atatgcatac cgttgccagc acacatgcat
     3301 atagcacatt gagtgattac attgcccagg ccaaacaaaa gggcattaaa ctttttgcga
     3361 tcaccgatca tggcccggat atggaagatg ctccgcatca ctggcacttc attaacatgc
     3421 gtatctggcc gcgagtggtt gatggggtag ggatcctgcg cggcatcgaa gctaacatta
     3481 aaaatgttga tggtgaaatt gattgcagcg gtaaaatgtt tgactcgctg gatctgatta
     3541 ttgccggttt tcatgagccg gtttttgcac cacatgacaa agcgaccaat acacaagcga
     3601 tgatcgccac tatcgccagc ggcaatgtgc atataataag ccatcccgga aatcccaaat
     3661 atgaaataga tgtgaaagcc gttgctgaag cagccgcgaa acatcaggtg gcgctggaaa
     3721 tcaataattc ttcattttta cactcacgta agggcagtga agacaactgt cgtgcggtag
     3781 ccgcagcggt acgtgatgct ggaggttggg tggcattagg ctcggattct cacaccgcgt
     3841 ttaccatggg ggaatttgaa gagtgtctta aaatcctcga cgcggtagat tttccgccag
     3901 agcgcatttt gaatgtttcg ccgcgccgct tactgaactt cc
//
LOCUS       DAPPNJ010000291         3863 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000291 DAPPNJ010000000
VERSION     DAPPNJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.293"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            18..749
                     /locus_tag="RPM73_004273"
     CDS             18..749
                     /locus_tag="RPM73_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDX6522540.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            774..3296
                     /locus_tag="RPM73_004274"
     CDS             774..3296
                     /locus_tag="RPM73_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6522541.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3307..>3863
                     /locus_tag="RPM73_004275"
     CDS             3307..>3863
                     /locus_tag="RPM73_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522542.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYG"
BASE COUNT         1074 a          814 c          921 g         1054 t
ORIGIN      
        1 ttggccaggg agtcgttatg tccagaaaaa gttgtttaat caggctcttt aaaacaacag
       61 tttcatgtaa gttatttacc gcgattatac tcagcgtttt tttaggccaa ccagcattaa
      121 cttatgcagg cgttgtcatt ggtggtacgc gggtggttta cttaagtaat aatgctgata
      181 aatctatttc tgtatttagc aaagaagaaa aaatacctta tctcatccag gcttgggtag
      241 atccatttaa taaagaggat aaaagcaaag cgccgtttac cgttattccg ccagtatcac
      301 gattagaacc ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc
      361 ctgatgatcg cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca
      421 acaaagcgac gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac
      481 cagcaaatat ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag
      541 ggcgaaatct gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg
      601 ttgatggcca tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt
      661 tacctgctaa ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag
      721 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
      781 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      841 tggccgaggt cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg
      901 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      961 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
     1021 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
     1081 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
     1141 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
     1201 cccaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
     1261 ggattaacgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
     1321 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
     1381 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
     1441 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
     1501 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
     1561 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
     1621 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg
     1681 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg
     1741 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1801 tggcgattct taaacgtgaa ggtcagacag atgttgatgt tagcgtgggt gaattgcgcg
     1861 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1921 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1981 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     2041 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaacgat
     2101 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     2161 ataccctcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     2221 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     2281 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     2341 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     2401 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     2461 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     2521 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     2581 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     2641 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     2701 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2761 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2821 gggccgccgg gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2881 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2941 tctcgaagtc gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg
     3001 cagtggttaa agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa
     3061 atgcaaatgg taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg
     3121 cagattcagg aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag
     3181 gacaggttac gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcac
     3241 tttcgacacc agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg
     3301 atttttatga acaagtatat caaacagtgg tgctttgctg tgtttatgct ctcgttaagt
     3361 agtgtagcac ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt
     3421 acgcttgatt tctcgggata tcttattacc gcgaacgaaa ataaggttgg aacgacattt
     3481 aacaccactg taactaatgg atcctcatat cctggacgct gtcattgtga tactggtaac
     3541 gtaggagaat tcccctatat ctattatacg tcaaaaataa accaggcatt aacttatgct
     3601 ggggttcact ctaatattaa ttattatgat cttaatccga accttgatgt tgggattgct
     3661 atagatattc ttggggttgg gtatgtgaat gcaccttttg aatatcatgc taacaacccg
     3721 tctggtaata caaagtacaa ttgcaaccgc attgaacctt taagtatatc tagtggggct
     3781 aaagcgatag tatattttta tatcaagaag acatttgcag gaaaattgat tattcctgaa
     3841 acaaagatag tgacattgta tgg
//
LOCUS       DAPPNJ010000292         3790 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000292 DAPPNJ010000000
VERSION     DAPPNJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.294"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..517
                     /locus_tag="RPM73_004276"
     CDS             <1..517
                     /locus_tag="RPM73_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522543.1"
                     /translation="VFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSIN
                     MPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIA
                     SSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDV
                     LSSLVMRVAQD"
     gene            597..1271
                     /locus_tag="RPM73_004277"
     CDS             597..1271
                     /locus_tag="RPM73_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX6522544.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1287..3767
                     /locus_tag="RPM73_004278"
     CDS             1287..3767
                     /locus_tag="RPM73_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX6522545.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSNYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
BASE COUNT         1095 a          708 c          936 g         1051 t
ORIGIN      
        1 tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg ataccggaac
       61 attaactatt aaggggaata ttgcagaatc tccgtgtaaa ttcgaagcgg gtggtgattc
      121 agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag ctaaatattc
      181 tacctatgat gatgcagtcg gtgtaaccag cagcatgtta aaaattagct gcccgaaaga
      241 agttgctggt gtaaaactct cgttgattac caacgataaa ataaccggta acgataaggc
      301 gatagccagt agcaacgata ccgtgggtta ctatctctat ttaggtgata acagcgatgt
      361 cctggatgtt tctgcacctt ttaacattga gagttataaa acagcggaag gtcaatatgc
      421 tattccgttt aaagcaaaat acctgaaact aacagataac tcagtgcaat caggtgatgt
      481 gttatcttct ctggttatgc gtgtggcgca ggattaataa ataaaattgc aagtttctcc
      541 aggtaataaa catggccgct atcccatggc ggccttttaa tttaagaggc attaaaatga
      601 aaggattatt atctttactc attttttcta tggtccttcc tgcacatgcc ggaattgtta
      661 tctacgggac gcgcattatt tacccggcag aaaataaaga agttatggtg cagttgatga
      721 accagggaaa tcgttcttcg ctgctgcagg cgtggattga tgatggcgat acgtcgttac
      781 caccagaaaa aattcaggtt cctttcatgt taacgccacc agttgcaaaa ataggggcaa
      841 attccgggca gcaagtaaaa atcaaaatta tgccgaataa actgcccact aataaagaaa
      901 gcatttttta tctgaatgtt ctggatattc caccaaatag tccagaacaa gaaggtaaga
      961 atgcactgaa gtttgcgatg caaaacagaa tcaaattgtt ttaccggccg gcgggtattg
     1021 ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa tcgcagtggc aatggattgg
     1081 tgataaaaaa tgactcagct aattgggtga cgatttcgga tgtcaaagct aataatgtca
     1141 aagtcaatta tgaaactata atgattgccc ccttagaaag tcagagtgtt aatgtcaaaa
     1201 gtaataatgc aaataactgg tatctgacca ttatcgatga ccacggcaac tatattagtg
     1261 acaaaattta attgcaggag ctgcctatgt tgagaatgac cccacttgca tcagcaatcg
     1321 tagcgttatt gctcggcatt gaagcttatg cagctgaaga aacctttgat acccatttta
     1381 tgataggtgg aatgaaagac cagcaggttg caaatattcg tcttgatgat aatcaaccct
     1441 taccggggca gtatgacatt gatatttatg tcaataagca atggcgcggg aaatatgaga
     1501 ttattgttaa agacaacccg caagaaacat gtttatcaag agaagttatc aagcggttag
     1561 gcattaatag cgataacttc gccagcggta agcaatgttt aacatttgag caacttgttc
     1621 agggtgggag ctatacctgg gatatcgggg tttttcgtct cgatttcagt gtcccgcagg
     1681 cctgggtgga agaactggaa agtggctatg ttccaccgga aaactgggag cggggtatta
     1741 atgcgtttta tacctcttat tatgtgagtc agtattacag cgactataaa gcgtcgggta
     1801 acaacaagag tacatatgta cgttttaaca gcgggttaaa tttactggag tggcaactgc
     1861 attctgatgc cagtttcagt aaaacaaata acaatccagg ggtgtggaaa agcaataccc
     1921 tgtatctgga acgtggattt gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca
     1981 catcaagcga tatttttgat tctgttcgct tcagtggtgt gcggttgttt cgtgatatgc
     2041 agatgttgcc taactcgaaa caaaatttta cgccacgggt gcaggggatt gctcagagta
     2101 acgcgctggt aactattgaa cagaatggtt ttgtggttta tcagaaagag gttcctcctg
     2161 gcccgtttgc gattacagat ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg
     2221 tgaaagaggc ggacggctcg gtaaccacct atctggtgcc ttatgcagcg gtgccaaata
     2281 tgctgcaacc cggcgtgtcg aattatgatt ttgcggcggg tcgtagccat attgaagggg
     2341 cgagcaaaca aagtgatttt gtccaggcgg gttatcagta tggttttaat aatttattga
     2401 cgctgtatgg tggctcgatg gtcgcgaata attattacgc gtttactttg gggactggct
     2461 ggaatacacg cattggtgcc atttccgtcg atgccacgaa gtcgcatagt aaacaagaca
     2521 acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt gtaagccaaa
     2581 cgtcgacgcg ttttggtctt gcggcctggc gttattcatc gcgtgattac cggacattta
     2641 atgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac gatatctatg
     2701 acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttttct gccaatatga
     2761 gccagtcatt gccagaaggc tgggggtctg tgtccttaag tacgttatgg cgagattact
     2821 gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac tggcggcgga
     2881 taagctatac cctcgcggca agccaggcgt atgacgagaa tcatcatgaa gagaaacgtt
     2941 ttaatatttt tatatcgatt ccctttgatt ggggtgatga cgttacgacg cctcgtcggc
     3001 aaatatatat gtctaactca acgacgtttg atgatcaggg gtttgcctca aataatacgg
     3061 gattatcagg aaccgttgga agccgggatc agtttaacta tggggtcaac ctgagttatc
     3121 agcatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg ccggttgcga
     3181 cagtgaatgg cagttatagt cagtcgagta cttatcgaca ggctggagcc agtgtttcag
     3241 ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg
     3301 ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa
     3361 caaaccgtaa tggagtggtg atatacgacg gaatgacacc ttatcgggaa aatcacctga
     3421 tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg aaaattgccg
     3481 ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc aagccctggt
     3541 ttataaaagc gttaagagcg gatgggcaac cattaatgtt tggttatgaa gtcaatgata
     3601 tccatggtca taatattggc gttgtcggtc agggaagcca gttatttatt cgcaccaatg
     3661 aagtaccgcc atcggttaat gtagcaattg ataagcaaca aggactttca tgcacaatca
     3721 ccttcggtaa agagattgat gaaagtagaa actatatttg ccagtaatag tcaggtggtt
     3781 atgaatattc
//
LOCUS       DAPPNJ010000293         3749 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000293 DAPPNJ010000000
VERSION     DAPPNJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.295"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            2..1387
                     /gene="dcuC"
                     /locus_tag="RPM73_004279"
     CDS             2..1387
                     /gene="dcuC"
                     /locus_tag="RPM73_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDX6522546.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1428..2108)
                     /gene="dpiA"
                     /locus_tag="RPM73_004280"
                     /gene_synonym="citB"
     CDS             complement(1428..2108)
                     /gene="dpiA"
                     /locus_tag="RPM73_004280"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDX6522547.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2077..3735)
                     /gene="dpiB"
                     /locus_tag="RPM73_004281"
     CDS             complement(2077..3735)
                     /gene="dpiB"
                     /locus_tag="RPM73_004281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDX6522548.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          858 a          988 c          903 g         1000 t
ORIGIN      
        1 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tagctcgcta
       61 catcattaaa gggtattccg ccactggtgt gttatttgtc ggtggcctgt tattgctgat
      121 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
      181 cgccacggat atcgttgaat acgttaaaat attactaatg agccgcggcg gcgacctcgg
      241 catgatgatt atgatgctgt gtggatttgc cgcttacatg acccatatcg gcgcgaatga
      301 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
      361 gattgccgcc tattttgtcg cctgtctgat gtctctggcc gtctcttccg caaccggtct
      421 gggtgttttg ctgatggcaa ccctatttcc ggtgatggta aacgttggta tcagtcgtgg
      481 cgcagctgct gccatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
      541 tgtggtgctg gcggcgcaag cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
      601 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
      661 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
      721 cactgctcct gcgttttatg ccattttgcc gttcacgccg atcatcggtg tactgatttt
      781 tgacggtaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
      841 tgcctccatt ctggagttcc tccgcagctt taatacccag aaagttttct ctggtctgga
      901 agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
      961 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
     1021 tacctcgttt ggttcggcga gtatcatcct gatgctggta ttggtgattc tgaccatgct
     1081 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
     1141 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actatcccga tgctgcaggc
     1201 gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta gtcgttgcgg ttgccgggat
     1261 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
     1321 agtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagcag cggtcacagg
     1381 caagtaaata gtaatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
     1441 tatatgcgtt gtggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
     1501 ctggcgcaat attcaagata acgcctggca gtggtgcgac tgatggtcag tgcctgcgcc
     1561 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
     1621 gtcaagggat caatgccggt cggcagttcg tctttaggtt caccgcgagc ataggcatta
     1681 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
     1741 tggcggaaac gggttagcgt ttgccccagc cgttcatagg caatgggctt aatgagataa
     1801 tcaaatacac cacaacgtac ggcttcagac accgtttcca tatcgctggc tgcagtggta
     1861 aacaccacgt cgccgggata atgcgcctgc accagttcat gcaataaatt aatccctctg
     1921 ccgtcaggaa gatagttatc gagcaagatt agccccggct taaaacgctc gatcatcatt
     1981 cgggcctgcg ccaggtttcc cgccagtaat atctgactga atccgggaat gtgacgaata
     2041 tattccgcat gcatctctgc cagcggcgtt tcgtcctcaa cgatcaatag ggttaatgga
     2101 gctgtcatta ggtttcactt tcggaatata gattgaaaac aaggtaccgc agggatcatt
     2161 atcttcgaga gtgataacac caccgcagcg cgttacgtag ctggcaatca agtacaaccc
     2221 aatgccatgt tcaccgggct cgtcagcacg cgtactgacc ccctgctcaa atattttgtc
     2281 tcgtagagac tctggaacgc cgcagccctg atcggcgact tcaatcacca catcatcgcc
     2341 ttcatcgctg aggaataatt caacgatctt gtttccttca tcgctacgca ggctggcttc
     2401 gaaggcgtta tcaagtaaat tgcccacaat ggctgcaaac tcggtgctat ccagtcctgg
     2461 cggcagttgc gaaagctggc taccggggac aatgatcatt tttagcccca gttcccgggc
     2521 gcgctgcact ttaccaaaaa gcagccccgc cacctggcga tcggcaaacg cctcgcgcag
     2581 gctgtcaata agctgttgct gggcctgaga ctccccctgc accatcgcca gcacgcgatc
     2641 atactctttc atctgcaaca gaccattgag cgtcgacatc caattgagat gctcgtgtcg
     2701 caatgtacga aggctctcga cgtattgttt tatttgcgtc agttgcgcat tgagggtgga
     2761 aatttcgtct ttactacgaa agctgataat ggcccccagc aaatcatcac ctgaacgaat
     2821 agcttcccga ttggcaataa cgctcagacc gttaaagtta gccaccacat cctgacgttt
     2881 ttcatcaatc tgttcggtaa agaaatcggc gggcctgacc acttcagcaa tgggtttacc
     2941 caaccattgc cgtccgggag agctcagccc cagcatcttt cttgcgttac gattgatggc
     3001 ggtaatgtaa ccatgcggat ccaccgcaat cagcccttca taaaccgaac taaacagcgc
     3061 ctcttgctga cggaccacgc gtgcgatttg ctttggctcc atgcccatca tttgccgacg
     3121 gatatgcgct gccaggaacc acgacagcaa catcagaatc cctaacagca cgacaaacac
     3181 gcctgccatc ggtaataaaa actcagcccg ccagctatcg attttactca ccaggtagcc
     3241 aatcgacacc acgccgatga cttttccatc gtcatcaaag attggcgttt tggcgcgcat
     3301 cgccatcccc attgaccctt tcccggtaat gaagtagctc tcccctttct ccagcgcgcc
     3361 ctgtttggtg aactgcatag gataaccaat tttctccgga ttaggatggt aaaggcggat
     3421 cgagtgccgg tccccaatca ccacataatc aaaatcggta tctctttgta atttgttagc
     3481 gatggtcgcc agccgtttgt agtcacgcgt tttcaccgca gagatgacac tgtcattgga
     3541 ggcaataatt ttcgcctgat tcattgccat gtcgcgtaca tgaagcgtta aatagtcctc
     3601 aaaactggcc gtaaaatatt gcgccagggc tgcaatgaca aatattgaga acaccagaat
     3661 cagcaaaaag atacgcagcg gaaatgccag tctttggaaa aatgcaaact gtttattctc
     3721 gttaagctgc aacattgttt tccttgcgg
//
LOCUS       DAPPNJ010000294         3710 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000294 DAPPNJ010000000
VERSION     DAPPNJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.296"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            94..540
                     /gene="hpaR"
                     /locus_tag="RPM73_004282"
     CDS             94..540
                     /gene="hpaR"
                     /locus_tag="RPM73_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDX6522549.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            659..2314
                     /gene="tsr"
                     /locus_tag="RPM73_004283"
     CDS             659..2314
                     /gene="tsr"
                     /locus_tag="RPM73_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDX6522550.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2363..>3710)
                     /locus_tag="RPM73_004284"
     CDS             complement(2363..>3710)
                     /locus_tag="RPM73_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX6522551.1"
                     /translation="ITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAMLLLFF
                     AAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLDRKGPR
                     LMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFNIKERG
                     RPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYRNREHV
                     ELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLYLAWLP
                     GYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKICIIAGM
                     FCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASVGSIQN
                     FASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDPRKD"
BASE COUNT          976 a          907 c         1027 g          800 t
ORIGIN      
        1 taaaatgaag aattgcaggg gcagcgcagt cccggtaacg tgatgattaa ctgtttctat
       61 attaatgatt ttgaattttt caggggaaag ataatgcacg actctctaac cattgcgttg
      121 ctccaggcgc gagaagcggc gatgagttat tttcgcccaa tcgttaaacg gcataatttg
      181 actgagcaac agtggcgcat cgtgcgtatt ctggcggaaa gcccatcaat ggattttcac
      241 gatctggcgt atcgcgcctg cattttgcgc ccaagcctga ccgggatcct tacgcggatg
      301 gaacgcgacg gtttagtgtt gcgattaaag ccgatcaatg atcagcggaa gctctatatc
      361 tcgctaacca aagaggggca ggcgctgtat aaccgcgctc agacacagat tgaagaagcc
      421 tatcggcaga ttgaagctca gtttactgcc gagaaaatgc aacagttaac ccatctgtta
      481 gaggaattta ttgctctggg caattcccga caagaagcca tcccgggcga taatgaataa
      541 ctttttagta tctgataacc agcgcctggt ttttccatgg atggcgggtt acaccttttc
      601 ataaagtttt tgctttccag gccgaaaatc ttgcatcggt ccacaggaaa gagaaaccat
      661 gttaaaacgt atcaagatcg tgaccagctt actgctggtt ttggccgttt ttggcctttt
      721 acaactgaca tcaggcggtc tgttctttaa tgccttaaag aatgacaaag aaaatttcac
      781 tgttttacaa accattcgcc agcagcaatc cacgctgaat ggcagctggg tcgcgttgtt
      841 gcagacgcgt aacaccctca accgcgcggg tatccgctac atgatggatc agaataatat
      901 tggtagcggt tcaaccgttg ctgagctgat gcagagtgcc agtatttcgc tgaaacaggc
      961 ggaaaaaaac tgggcggatt acgaagcgtt gccgcgtgac ccgcgtcaga gcaccgccgc
     1021 agcggcagag atcaaacgta attacgatat ttatcacaat gcgctggcgg agctgatcca
     1081 actgttaggt gcaggcaaaa tcaacgagtt ctttgatcag ccgacccagg gatatcaaga
     1141 cggtttcgag aagcagtatg tggcttacat ggagcaaaac gatcggctct atgatatcgc
     1201 cgtcagcgat aacaatgcct cctacagcca ggcgatgtgg attctggtgg gcgtgatgat
     1261 cgtcgtactg gcggtcatct tcgccgtctg gttcggtatt aaagcctcgc tggtagcgcc
     1321 aatgaatcgc ctgattgaca gcatccgtca tattgcaggc ggcgatctgg tgaaaccgat
     1381 tgaggtggat ggctctaatg agatggggca actggcagag agtttgcgcc atatgcaggg
     1441 agagctgatg cgtaccgtcg gtgatgtgcg caacggggcc aatgccatct atagcggtgc
     1501 cagcgaaatc gctaccggca ataacgatct ctcttcgcgc accgagcaac aggccgcttc
     1561 gctggaagag acggcagcca gcatggagca actgaccgca acggtgaaac agaacgccga
     1621 gaatgcgcgc caggccagcc atctggcatt aagtgcttct gaaacggcgc aacggggcgg
     1681 caaagtggta gataacgtgg tgcagactat gcgcgatatc tccaccagtt cgcagaaaat
     1741 cgccgatatt atcagcgtaa ttgacggcat tgccttccag accaatattc tggctttgaa
     1801 cgcggcggtt gaagcagcgc gtgcgggtga gcaagggcgc ggttttgcgg tggttgcggg
     1861 agaagtgcgt aatctggccc agcgtagcgc tcaggcggct cgtgaaatta aaagccttat
     1921 tgaagactcg gtggggaaag tggatgttgg ctctacactg gtcgaaagcg ccggggaaac
     1981 aatggcggag attgtcagtg ctgtgacccg cgtgacggac attatgggcg aaatagcttc
     2041 tgcttctgat gagcagagcc gtggtatcga tcaggttggc ttagcggttg ctgagatgga
     2101 ccgggtaact caacagaacg ccgcgctggt ggaagaatct gccgccgccg ccgccgcgct
     2161 ggaagagcag gccagtcgcc tgaccgaagc agtggcagtg ttccggattc agcaacagca
     2221 gcgtgaaaca tcggctgtgg taaaaaccgt gacgccagct gcgccgcgta aaatggccgt
     2281 ggcagatagc gaggagaact gggaaacatt ttaatcgcca tgaaaatgcc cgataagcaa
     2341 aatgttatcg ggcataagga gattaatctt tacgtgggtc gttgatcggc tgacgaacca
     2401 ggaagatgta cgccagtgcc cccgctgcgg tgacgcaacc gcagatgatt agtgccagac
     2461 ggaatgagtg ggtggtatca acaataaaac cagtaatgat cggcgcaaaa gaggcgcaga
     2521 tgaagctggc aaagttctgg atactgccca ccgacgcagt catgcgagaa gcaactgcga
     2581 cgtggatcaa gccccagcag gatgttccgg caaagtgaat acagaacagt gccatgccaa
     2641 tcagcagaac cgccgtcatg gatgttgtcg cttgtggtac gaccagcgta aaggcggcag
     2701 aacagaacat cccggcaata atgcagatct tacggctttt aatcggagcc attccccctt
     2761 tgaccagcca gtcagtaacg taaccgttga ccagcatccc ggcagcccca aacaggaaag
     2821 ggatagccgc catcaaccct gtgcttttta aatccaggtt ataggctgtt tgcaggtaac
     2881 caggaagcca ggccagatac agccacgcag tgtagttgat gccactgaat ccgagcatca
     2941 ttccccacat tgtacggtta cggaacaggc tgcgccattc ggcaaaactg agcggatctc
     3001 ggcgggcatt gacgctacct gcattgagat aagcttgttc aacggcagtc agttctacgt
     3061 gctcgcggtt gcgatagagc atataccagc cgatggcgag aaaaatcccc agtacaccaa
     3121 tggtaataaa catcccgcgc cagcccatca ccagcatcat cgccgccaga atcggtgggc
     3181 ttacggcaac gccaatggta gaagctgcgt tgaagaagcc catcgggcgt ccgcgctctt
     3241 tgatgttgaa ccagtcgtta atgactttta caccgcatgg gttcatcggc gcttcaccaa
     3301 tccccatacc gatacgcacc aacacgaact gcgtaaagtt gtgcaccatg ccagacattg
     3361 cctggaacag tgaccagaag aacatcccca gtcccagcat caggcgtggg cctttacgat
     3421 ccaatagtgg gccgcaagga agttgcgcaa tcccgtaagc gagtgaaaac acggagagca
     3481 aagcgccgat ttcggtggca cttaatccca attcttcacg aatcgttaaa tttgctaccg
     3541 acagcgaact gcggtcgaga taattgatta ccgccgcaaa aaataataac aacatggcgg
     3601 tggtttgaat gcgtttaatt cgggtactgc gttgaactaa tccatctggt ggcacgggaa
     3661 tatctgccga cgaagatgga gtaaatgaag aacgcggatc gagggtgata
//
LOCUS       DAPPNJ010000295         3706 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000295 DAPPNJ010000000
VERSION     DAPPNJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.297"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..217
                     /locus_tag="RPM73_004285"
     CDS             <1..217
                     /locus_tag="RPM73_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522552.1"
                     /translation="VFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILFKMRE
                     DAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(294..536)
                     /locus_tag="RPM73_004286"
     CDS             complement(294..536)
                     /locus_tag="RPM73_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDX6522553.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            889..1809
                     /gene="lpxP"
                     /locus_tag="RPM73_004287"
     CDS             889..1809
                     /gene="lpxP"
                     /locus_tag="RPM73_004287"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDX6522554.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2165..2236
                     /gene="ypdK"
                     /locus_tag="RPM73_004288"
     CDS             2165..2236
                     /gene="ypdK"
                     /locus_tag="RPM73_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDX6522555.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2301..3539)
                     /gene="alaC"
                     /locus_tag="RPM73_004289"
     CDS             complement(2301..3539)
                     /gene="alaC"
                     /locus_tag="RPM73_004289"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDX6522556.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          961 a          946 c          872 g          927 t
ORIGIN      
        1 tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag agactgaaaa
       61 aacatcagcg atgacaacca cacactctac aaaacttcag ccatcacagg cgattttgtt
      121 taagatgaga gaagatgcgc caccattaaa cctcacagaa gaaatcacgc ctacttatcc
      181 gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg gcaggtgaac
      241 aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac ccactatcgc
      301 ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag aaacataaat
      361 atccccgtca ctagttcatt accgccagtt aaccacatgg tgaagtaata aatgagtgca
      421 gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag tacctgaccg
      481 atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata aatcatagct
      541 caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta agacaatcct
      601 taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg caagactgtt
      661 gtgtacggaa aaatatttac tttgcacgat taataatcag tagctgaaag cagtcagcga
      721 gtgataaagt acagataccg cattcatgct cctcgtggtt atgtcctgac tagtctttac
      781 actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt accgctgtcg
      841 acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat gtttccacaa
      901 tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt tgggcttggt
      961 gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg cacgcgtatt
     1021 ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa aaacctggaa
     1081 ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga aaactttcgt
     1141 tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga cagtcgcgta
     1201 cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat gcaaaatcgc
     1261 ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg cgtgatggga
     1321 ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat ggaatgggtg
     1381 cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc
     1441 attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca ggattatggt
     1501 cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac aaccaatggc
     1561 acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat ggtaagaaaa
     1621 gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta cccgacagat
     1681 gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagagatcat gcgcgcaccg
     1741 gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgttg
     1801 tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat aacctcatgg
     1861 tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta ttaccgtcag
     1921 aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgt
     1981 ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga
     2041 attctggggt gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca
     2101 actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg
     2161 taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt
     2221 tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt
     2281 tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt
     2341 agtaaaccgt cggcacggaa catcgcttta atcccacgaa tcgcctgacg aatacggtcg
     2401 cggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc
     2461 ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg
     2521 gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg catttcaacc
     2581 atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt gtactgttcg
     2641 gcaatgtcgc gcacgcactg ttgatcgccc tccagcgccg caatcgccgc cacctgcaac
     2701 ggcgtaaagg tgccgtaatc gtgatagctt ttaatacgag ccagggcgct gaccagcgtt
     2761 ttgttgccaa ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta
     2821 aagaactcga ctgccacatc gcgtgcaccc ggtacctgca tgattgacgg cgctttccag
     2881 ccatcgtaga cgatatcggc ataggccagg tcgtggacca ccagcacatc gtagcgtttc
     2941 gccagcgcca caaccttttc gaagaactcc agctccacgc attgcgcggt tggattcgac
     3001 gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt
     3061 tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc
     3121 accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga
     3181 tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct
     3241 tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga
     3301 cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca
     3361 gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa
     3421 tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa
     3481 acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata
     3541 atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct
     3601 gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat
     3661 ggctttaaat gaggaactcc acttcaaaaa caggaaattt ttctac
//
LOCUS       DAPPNJ010000296         3686 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000296 DAPPNJ010000000
VERSION     DAPPNJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.298"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1267
                     /locus_tag="RPM73_004290"
     CDS             <1..1267
                     /locus_tag="RPM73_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDX6522557.1"
                     /translation="TLAKAGWRVALIEQSNAMYGGTCINIGCIPTKTLVHDAQQHTDF
                     VRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFINNHSLRVHRPEGNLEIHGEK
                     IFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANF
                     GSKVTILEAASLFLPREDRDIADNIATILRDQGVDIILNAHVERISHHENQVQVHSEH
                     AQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVVDKRLHTTADNIWAMGDVTGG
                     LQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGAD
                     IQVVTLPVAVIPRARVMNDTRGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDA
                     GLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            1376..1612
                     /locus_tag="RPM73_004291"
     CDS             1376..1612
                     /locus_tag="RPM73_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX6522558.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DIIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            1624..2217
                     /gene="rclC"
                     /locus_tag="RPM73_004292"
     CDS             1624..2217
                     /gene="rclC"
                     /locus_tag="RPM73_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDX6522559.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2377..2505)
                     /locus_tag="RPM73_004293"
                     /pseudo
     CDS             complement(2377..2505)
                     /locus_tag="RPM73_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            2808..3665
                     /locus_tag="RPM73_004294"
     CDS             2808..3665
                     /locus_tag="RPM73_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX6522560.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYSEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
BASE COUNT         1054 a          771 c          815 g         1046 t
ORIGIN      
        1 cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa caatcaaatg caatgtatgg
       61 cgggacctgt attaatatcg gctgcatccc aaccaaaaca ttggttcatg acgcacagca
      121 gcacacagat tttgtccgtg ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa
      181 taagaatttt cataatcttg cggatatgcc caatatcgac gtgatcgacg gccaggcgga
      241 gtttatcaat aatcatagcc tgcgtgttca tcggcctgag ggaaatctgg aaattcatgg
      301 cgagaaaatt tttattaata ccggtgcaca aaccgtggtt ccgccaattc ctggaattac
      361 caccacgcct ggtgtatatg acagcaccgg attacttaat ctaaaagaat tgcctgggca
      421 tttaggtatt ttgggcggcg gatatattgg cgttgagttc gcctctatgt tcgctaattt
      481 tggcagcaag gtaaccattt tagaagcagc ttcgctgttt ttgcctcggg aagatcggga
      541 tattgctgat aatatcgcga cgattttacg cgatcagggc gtcgatatta tcctcaatgc
      601 ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca
      661 actggcggtg gatgcactgt taatagcttc cggtcgtcaa ccggctaccg cttcgttaca
      721 tccagaaaat gccggtatcg cagtaaacga gcgcggggca attgtcgttg acaagcgatt
      781 acataccacc gcagacaata tttgggcgat gggagatgtt accggcgggc tgcaatttac
      841 ttacatatca ctggatgatt accgcattgt acgtgatgag ttactgggtg aaggcaaacg
      901 tagtactgat gatcggaaaa atgtgcctta ttccgtattt atgacaccgc ccctgtccag
      961 ggttggtatg acagaagaac aagccagaga gagtggtgct gatattcagg tggtgacatt
     1021 gcctgtagct gtaattccgc gcgccagagt gatgaacgat acccgtgggg tattaaaagc
     1081 aattgttgat aataaaaccc aacgtatatt aggggcatca ctgctgtgtg ttgactccca
     1141 cgagatgatt aatatagtga aaatggtgat ggatgccggg ctgccttata gcatattacg
     1201 cgatcagata tttactcatc cgtcgatgag cgaatcactc aatgatctat tttcattagt
     1261 caaataaact caaaatcaga cgccagaaca aatattctgg cgtctcagag aaaagaatct
     1321 tattaattct ctgttactct ataacttctt aatcacttca ttgatggtat tttatatgtt
     1381 taaaaaatct gttttatttg caacactatt atctggcgtt atggcatttt ccaccaatgc
     1441 agatgataaa ataattctga aacatatcag cgtctcgtca gtatcagcat caccgacagt
     1501 tctggaggat atcattgctg atatagccag aaaatataat gcttcatcct ggaaagtcac
     1561 atcgatgcga attgataata attcaaccgc aacagcagta ttgtataaat aaggatgttc
     1621 acaatggaaa aatacctgca cctgttaagt cggggagata aaattggcct gacattgatt
     1681 cgtctgagta ttgcaattgt ttttatgtgg attgggttat taaagtttgt cccttacgag
     1741 gcagacagca ttacaccatt cgtcgcaaac agtccactaa tgtcgttctt ttatgaacac
     1801 ccggaagact ataaacagta tctgactcac gaaggcgaat acaaaccaga agcaagggca
     1861 tggcaaacgg ccaataatac ctatggtttt tccaacggtc ttggcgtcgt ggaggtgatt
     1921 attgctctgc tggttttggc taatcctgtc aatcgctggt taggtttatt gggaggactg
     1981 atggcattta ccacgccgct ggtaacactc tcatttttaa tcaccacccc ggaggcatgg
     2041 gtacccgcat tgggtgacgc tcatcatggt ttcccttatt tatccggtgc tggtcgcctg
     2101 gtattgaaag atactctgat gctggcaggt gctgtcatga taatggcaga ttcggcgcgg
     2161 gaaattctta aacaacgcag taatgaatcc agttcaacgt taaaaactga atattgataa
     2221 tctgaacact gtctgttgtt cactttttca ggcgtcaggc cccctttctg atgatgaagc
     2281 ctgacgccat attaccggag atatgacaga gtgaagatca atatcactcc cctgttaaaa
     2341 ggtttttgtc gtcttaggta aaatacatga ttaatatcaa tcatgtattt ttacagcaag
     2401 acaaccacga acaaattcag gattaaaatg ttttaccgac tccccaacat aacccaaatc
     2461 aagcgaacta atttgtgcca tttctttatc tgtcagtgag aaatcgattt ttgatgggca
     2521 tctgcaatac tctgaagcag actatacgga aaattccact gtcagtccct ccattaggca
     2581 tgaacaatga gtctacgtta aaacgtaacc tcaaagtagt atgtggattt tgatatcact
     2641 tatgcaaaaa attcattaat aatgtaggac tgaaacctct ctattttcgg ggacaacgaa
     2701 gcagacgcta ccagtgcttt tgccttcgcc cttgctgttt ttgatacact tagggcccag
     2761 ggcataacga aaatgtgcga tatgacaaag aattaacgga gaatgtgatg atcaggcaga
     2821 agattctaca gcagctcctg gagtggattg agtgcaatct tgagcaccct atttcaatcg
     2881 aagatatcgc acagaaatct ggctacagca gacgcaacat ccagcttctg ttccgaaatt
     2941 tcatgcatgt gcctttggga gaatacattc gcaaacgaag gctttgccgt gccgctattc
     3001 ttgtccggct caccgcgaaa tctatgcttg atattgcact ctctttgcat tttgattcac
     3061 agcagtcatt cagccgcgaa tttaaaaagt tattcggctg ctctccccgt gaataccgcc
     3121 accgtgatta ttgggatctc gcaaatatct tcccttcttt tttaatacgt caacagcaaa
     3181 agacggagtg tagattagtc aattttcctg agacacctat ttttggcaac tcatttaaat
     3241 atgacattga agtgtcgaat aaatcaccgg atgaagaagt caaactgcga cgtcatcatt
     3301 tagccagatg tatgaataat tttaagacgg atatctattt cgtctccacg tttgaaccgt
     3361 caacaaaatc ggtcgatttg ctcacggttg aaacttttgc cggtacggta tgcaaacaaa
     3421 ccgattcaga gatgccgaaa gagtggacaa ttaaccgagg actgtatgcc tctttccgct
     3481 atgaaggtga gtgggagcac tactctgaat gggcgcgaaa cctctatctg atggaattac
     3541 ccgccagggg gttagccaga gtgaacggca gtgatattga gcggttttat tacaatgaag
     3601 atttcgtaga aaaggatagc aatgatgttg tttgcgaaat ttttatcccc gttcgtccgg
     3661 tttagctgtc gctatatcta aatgaa
//
LOCUS       DAPPNJ010000297         3669 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000297 DAPPNJ010000000
VERSION     DAPPNJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.299"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            86..736
                     /gene="csgD"
                     /locus_tag="RPM73_004295"
     CDS             86..736
                     /gene="csgD"
                     /locus_tag="RPM73_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDX6522561.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            741..1130
                     /gene="csgE"
                     /locus_tag="RPM73_004296"
     CDS             741..1130
                     /gene="csgE"
                     /locus_tag="RPM73_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDX6522562.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1155..1571
                     /gene="csgF"
                     /locus_tag="RPM73_004297"
     CDS             1155..1571
                     /gene="csgF"
                     /locus_tag="RPM73_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDX6522563.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1598..2431
                     /gene="csgG"
                     /locus_tag="RPM73_004298"
     CDS             1598..2431
                     /gene="csgG"
                     /locus_tag="RPM73_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDX6522564.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2495..2986)
                     /locus_tag="RPM73_004299"
     CDS             complement(2495..2986)
                     /locus_tag="RPM73_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDX6522565.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3088..3642)
                     /gene="ycdY"
                     /locus_tag="RPM73_004300"
     CDS             complement(3088..3642)
                     /gene="ycdY"
                     /locus_tag="RPM73_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDX6522566.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
BASE COUNT         1016 a          892 c          842 g          919 t
ORIGIN      
        1 gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc aggtgtgcga
       61 tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc atggtcatac
      121 attattgttg atcactaaac cgtctttgca agcgacagct ctcttgcagc accttaaaca
      181 atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg atatatcttc
      241 aggctctatt attcttctgg atatgatgga agcagataaa aagcttatcc attattggca
      301 ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata cgcctgaaga
      361 ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt atgccatgga
      421 ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgcggcgaat gctactttac
      481 gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata acagcacgga
      541 atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc
      601 taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa cgcatcttta
      661 taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga
      721 taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg gcagaatttc
      781 tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg ttaactgacc
      841 atactgtttc atctattggc catgattttt accgagcctt tagtgataaa tgggaaagtg
      901 actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga agctggatca
      961 ctataacggt caatcaggac gttattttcc agactttttt atttccgttg aaaagagact
     1021 tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat cgtcgccaga
     1081 taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa ataaaaaatt
     1141 gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt atttcgccat
     1201 taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt ggtaacccaa
     1261 ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa gatccgagct
     1321 ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact caggccatcc
     1381 agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg ggccgcatgg
     1441 tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag ttgaacgtga
     1501 cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa aataactcaa
     1561 ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat ttcttttggt
     1621 tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg ccagaccgac
     1681 attaatgcct cgagctcaga gctacaaaga tttgacccat ctgccagcgc cgacgggtaa
     1741 gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac cctacccggc
     1801 aagtaacttc tccactgctg ttccgcaaag cgccacggca atgctggtca cggcattgaa
     1861 agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc ttaacgagcg
     1921 caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc gaatcccgct
     1981 gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt atgaaagcaa
     2041 cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca cgcaatacca
     2101 gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg agatcctctc
     2161 ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg ttttccgctt
     2221 tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg aacccgttat
     2281 gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg atggtatcga
     2341 ccgtggtctg tgggatttgc agaacaaagc agaacggcag aacgacattc tggtgaaata
     2401 ccgccatatg tcggttccac cggaatcctg acagataaaa aagccggaat tatcatccgg
     2461 cattttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg atttcctgct
     2521 cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt ttcattgcgt
     2581 aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca ccctgcccgg
     2641 caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg
     2701 cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga atttccagat
     2761 gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca ctaagcaggg
     2821 ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag taggccgtac
     2881 agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat ccccagatac
     2941 cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg cgcatgatag
     3001 cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca aattatctgt
     3061 tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca gctcgtccca
     3121 cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag gcgtggttgc
     3181 atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact cactgaacag
     3241 tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg aagccgcaag
     3301 cagcaatgtg ccgatgtgat cggctggcgt atccgccagt ggcatccctc gttcggaaag
     3361 aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat atggcggcac
     3421 ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg ccatatcaca
     3481 gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg cagccagttt
     3541 cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt ggcggtaata
     3601 gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat ttccattact
     3661 agtaattaa
//
LOCUS       DAPPNJ010000298         3619 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000298 DAPPNJ010000000
VERSION     DAPPNJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.300"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(217..1374)
                     /gene="intS"
                     /locus_tag="RPM73_004301"
     CDS             complement(217..1374)
                     /gene="intS"
                     /locus_tag="RPM73_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDX6522567.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGR
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(1536..1610)
                     /locus_tag="RPM73_004302"
     tRNA            complement(1536..1610)
                     /locus_tag="RPM73_004302"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1575..1577),aa:Arg,seq:cct)
     gene            complement(1686..2618)
                     /locus_tag="RPM73_004303"
     CDS             complement(1686..2618)
                     /locus_tag="RPM73_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDX6522568.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2912..>3619
                     /gene="mlaA"
                     /locus_tag="RPM73_004304"
     CDS             2912..>3619
                     /gene="mlaA"
                     /locus_tag="RPM73_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDX6522569.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENP"
BASE COUNT          854 a          878 c          857 g         1030 t
ORIGIN      
        1 aatctaataa aaattattaa tggtttcgca atcgaattct agcattaaaa tttttttcga
       61 tcgacaaacg aagcagtgtt tgtggtaatc atatactacc ataccacaaa tcaaagttat
      121 ggttatctgt agatagcctt taacaaaaaa tagtgtcaca caaatctttg cactggattg
      181 caaggctttg tgctcttcga taatggttaa ggtggatcac tccacctttt catcaatcca
      241 gtccgcccac cattgcatca tttctctgcg tttatcgaga tactgagcat ggttgtaaat
      301 tccgcgcaca gatccgccgt tggcatgtgc aagttgcact tcaatagcgt cagcaggcca
      361 ttcgtgctcg ttcataatcg tgctgaattc atgcctgaat ccgtgaccgc tttccagacc
      421 ctcatagccg atttgtttga tcacaagcaa taccgcgttc tcgcagattg gcttcttctt
      481 atcgttgcgc ccggcaaaaa caaactctga tactggtttg gtgattgagc ttagtgtagt
      541 gagaagttca accacctggt ctgacatcgg gaccacatga attttgcgtc ccttcatcac
      601 actggcgtcg atggtgataa tcctgttttc aaaatcgacg ttcttccatt gcatggaacg
      661 aagctctttc gttcttaggg ctgtgtagcg taaaactttg gtcgcaatga gcgatacgat
      721 tcttcctgaa aatgttgcca gtgctttgtt gaatgcaggg atctggtctg caggaagaaa
      781 cgggaagttc ttcttgcggt atcccttcat ggcgtcagca aggtcaggtg ccgggttata
      841 tttagcccta ccagtgacaa tagcgtaacg gaaaacctcg ccgcatcttc tgcgggcttt
      901 gttggctcgc tccattgcac cgcgatcttc aaatctgcgg attacttcca gcagttgcat
      961 aggctcaata tcctgaattt caagaccgcc gatgatgggt aaaatgtcgt catcaaacat
     1021 ttttgcaagt tcagtcgcat accctactga ccagacttgc ttcttgtgtt cgtaccattc
     1081 cttgtaaatt gcactaaagg aattgttgtt agacgaagcc tttttcgctt ttaccggatc
     1141 gatgccaacc gagatgtctt tcctcgcagt ccatgcctta tcccttgctt cctgcaaagt
     1201 cataagcgga tattttccga cagtcaggat tttctcctta ccgtcaatct tgtagcgaag
     1261 ctgccatacc tttttccctg atacagggac ataaaggtac aggccattac catcgagtag
     1321 gcggtatggt ttttctttcg gctttgctgc ttcaatctgc ttaacggtga gcatgggtaa
     1381 aaatccggtg ggtaaaatta ttttatccac tttttacccg tcatggagtg cggctgtcaa
     1441 cgatctgacg cgaaccatta cgaactgtta atctacggaa ggcttgatat tcaggggatt
     1501 ttgcggactg gtacggatgg gagcgaactg ataaatggcg tcccctgcag gaatcgaacc
     1561 tgcaattagc ccttaggagg ggctcgttat atccatttaa ctaagaggac aatgcggcat
     1621 gagtataccc gctaatggac tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc
     1681 gccatttatg ctgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttgg
     1741 cgtgcttctg ctttacgctt gttgctcatg tcgttacgaa tctgtgcatg actcattaac
     1801 gcgaagataa aggtgccgcc gcagatgttc cccgctaaag taggtagtgc gaagggccag
     1861 atgaaatcgc tccagtgcag cgtgccgtta aacaccagat agaggatttc aacagaaccg
     1921 acaacgatgt gggtggtgtc acccagtgca ataagccagg tcatcaatat aatcaccaca
     1981 atctttgccg cacccgcagc aggaaacatc caaaccatag tggcgatcag ccagccggaa
     2041 atgatcgcgt tggcaaacat ctcgctgggg gtgttcttca tcacatccat gccgattttg
     2101 acaaatgcat cgcgagtttc ttcattgaag ataggcatat attcaaatgc ccatgcagca
     2161 atacctgtcc cgagaatatt acccagcagc acgacgcccc ataatcgcat aagtaagccg
     2221 acgttgctca ttgtcggttt ttgcatgacg ggtagtaccg cagtcacggt gttttcggta
     2281 aataattgct ggcgggccat aatgacgata ataaaaccaa aggtataacc gagattctcc
     2341 agtaagaagc tgcctggcac tccttccagt tcgacatgaa atatcccttt tgccagtagc
     2401 gaagcgccca tcgacagacc cgccgcaatg gctgaccaca gtagcgccat tgcgtcgcgt
     2461 tccagctctt tttcaccatc ctggcggata tgctcatgaa ttgccatcgc ccgggagggg
     2521 agtcgatctt catctatttc tatttttttg ccgcgctctt tttcttcgct ctcaacttca
     2581 atttcgtcgc tgtgttgatc aattttgtcg ttgtccatgg tctcttctct tgaattagca
     2641 cgtatagcta aagcgtagcg gcttttttgc tcgcaactgg cgggagttac tctgaaaatg
     2701 tagaaaaggc tgcgtttgcc tttttctgtt tctatagaat caagtagcct acagggcggc
     2761 gattaccagg ctatgatcaa atcagcaaat cagggcgtct ggacatcagt tgacgtgctg
     2821 ttacaatcgc ccacacctaa acaggcggat acggtatcgt tccgtcatgg atggcaaact
     2881 gcataagcca taaaaaaaca gggagacatt tatgaagctt cgcctgtcgg cgcttgctct
     2941 gggaactacg cttctggtgg ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga
     3001 cccgttagaa gggttcaacc gcaccatgta caacttcaac ttcaacgtat tagacccgta
     3061 tattgttcga ccggtcgctg tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg
     3121 tttgagcaac tttactggca accttgaaga acctgcggtg atggttaact acttcttgca
     3181 gggcgaccct tatcagggga tggtccactt tacccgcttt ttcctgaaca ccattttggg
     3241 gatgggcggc tttattgatg ttgcagggat ggcgaacccg aaactgcaac ggactgaacc
     3301 tcaccgcttc ggtagtacgc ttggtcatta tggcgtgggt tatgggcctt acgttcagtt
     3361 accgttctac ggtagcttca cgctgcgtga tgacggtggt gatatggcgg atggtcttta
     3421 cccggttctt tcctggctga cctggccgat gtctgtgggt aaatggacgc ttgaagggat
     3481 cgaaacccgc gctcagctgc tggattccga tggtctgctg cgtcagtcgt ccgatcctta
     3541 tattatggtg cgcgaagcgt acttccagcg tcatgatttc atcgctaatg gcggcgaact
     3601 caaaccgcag gaaaatccg
//
LOCUS       DAPPNJ010000299         3463 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000299 DAPPNJ010000000
VERSION     DAPPNJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.301"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            28..>3463
                     /locus_tag="RPM73_004305"
     CDS             28..>3463
                     /locus_tag="RPM73_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDX6522570.1"
                     /translation="MPLFRKNLITVSLLLSVFPGISWADRVDVPVYVEESLGPNWQSM
                     TIGTTSTSTSGMVPPVAHGTRSIAIGNNAVAGTEIEKPQVGEAIAIGSKANATKEQSV
                     AIGADTNATGWGSVVIGGDDIRPVHGKTYGRLTIPNAFLASTASGTGSVAIGSRTLSG
                     GELSLALGSIALAKGDASTAIGATAEAQDERSTAIGFNALSMARSALALGSESSATSE
                     SAMAFGSRAIAGKSSSIALGTDAVVSVGDNNVALGAGSQDKAATSVSSASVRSSDGRL
                     VTYQGFAASDPSSVISVGAQGKERQIVNVAAGAVTENSTDVINGSQLYSVAKSLQDDI
                     NAVTSAGFALKDQGNNEVRQVSGSAIQITGDDNITTTATDSDDNKSLKISLNDDLTVG
                     HDKAGSITVNNAAGNSSISLNGGDGTISLTSPAASGDTSTTATLGVTTGQGGLTDADG
                     TTKTRLIYTPDGSSEPEELASMNDGLSFVGDDGNVINKKLNETLSVTGGAGADAEMTD
                     NNIRVDNREGGLRVRMTRNLRDLASAVFGNGDNITTITDNGMSVTTGTGDNVRTVSLG
                     KEGLNNGGNIITHVASGLNGKQLSEADGDTLTNAANIGDLKSALQDTTSALVNSGYGV
                     QADDGNKVTRPLGEHVSIAGDGRNITTMVQDGKLLVQLSEHIDTGKNGSVTVGDTVIT
                     DEGLSITGGPSVTKSGINAGDQVISGVQDGVDAHDAVNVSQLNQQINNATAATTWSLK
                     TESSDASVAVSSQTVEVKHGLNTRVSAVQTDDKGNYSYEIDVTGIPVEYTDSKGNPLV
                     NIGGKFYTRTEDPDSGNITLSPAEPARVRISSDQPLVLTSVADGAVTPESVDAVNGGQ
                     LASAGNIVAGKNVVWNNGKATLSPDTFSELTTADGSNRTTTGTIQTVPDNVADAVNML
                     NREGTKYFKASSSGPAAKAEGRNSVAIGQSAISRAENSIAMGNGAEVSGDAAAGSVAI
                     GSGSRAGRAHTGNYSLNGQVVAGRTGSNTPVMSFGQAGQERQLQHVAPGVLSAESTDA
                     VNGSQLYAVHRQTEGNTQAINNLGKKFDHLNDRVDELNRDIRGVGASAAAMSGIPQAY
                     LPGKSLMGLGVGGYGGESAVAIGVSRISDNGKLIMKMNAGQNSRGNFSVGAGVGWQW"
BASE COUNT          909 a          816 c          991 g          747 t
ORIGIN      
        1 aacaccattg ttatttacgg gtgatgtatg cctttattca gaaaaaacct gattacagtt
       61 tctctcctgc tgtcagtttt ccccgggata tcctgggcag atcgcgttga tgttcctgta
      121 tatgttgaag aatctctggg gccaaactgg cagagtatga ccattggaac cacatccaca
      181 agcacatccg gaatggttcc tccggtagcc cacgggaccc gttcaattgc cattggaaat
      241 aacgctgttg ctggcactga aatagaaaag ccccaggtgg gtgaagcgat cgccattggc
      301 tccaaggcaa atgctacaaa agagcagtct gtggcaattg gtgctgacac caacgccact
      361 ggctggggga gcgtcgtcat cgggggcgac gatatacggc ccgtacacgg aaaaacttat
      421 gggcgcctga ccatcccgaa tgcctttctg gcttcaacag cctcaggaac aggttctgtt
      481 gccattggca gccgcacgct ttccggtggg gagttgtcgc tggctctggg ctccatcgcc
      541 cttgcgaaag gtgatgcctc cacggctatc ggtgccaccg ctgaagcaca ggatgaacgt
      601 tccaccgcca ttgggtttaa cgccctgtcc atggccagaa gtgcactggc tcttggcagt
      661 gaatcatctg caacctccga gagtgccatg gctttcggca gcagggccat tgccggaaaa
      721 tcatcttcca ttgcattagg cactgatgca gtggtttcgg ttggcgacaa taacgttgcc
      781 ctgggggccg ggtcacagga taaagcggcc acaagtgtga gcagtgccag tgttcgctca
      841 tcggatgggc gtctggtcac ctatcagggc tttgccgcca gtgatccatc ctctgttatt
      901 tctgttggcg ctcaggggaa ggaacgacag attgtgaatg tcgcagccgg tgccgtaaca
      961 gagaacagta ccgatgtcat taacggcagt cagttgtatt ctgtggccaa atcccttcag
     1021 gatgacataa atgctgtcac tagtgccgga tttgcactga aagaccaggg aaataatgag
     1081 gtcagacagg tgtcaggcag tgcaattcag atcaccggtg atgacaatat caccaccacc
     1141 gcgacggaca gtgatgataa taaatcactt aagatttccc tcaatgatga ccttacagtg
     1201 gggcatgaca aagcggggag tataaccgtc aataatgctg caggtaacag cagtatcagc
     1261 ctgaacggag gggacgggac aatttctctg acctcacccg cagcttccgg tgatacatcc
     1321 accacagcaa cacttggtgt gaccacgggg cagggggggc tgacggacgc agacggcacc
     1381 acgaaaaccc gtctgatcta tacacctgac gggagcagtg aaccggaaga actggcctcc
     1441 atgaatgacg gtctctcttt tgtcggtgat gacggaaatg tgattaataa aaaactgaat
     1501 gaaacactgt cagtgacggg gggagccggc gcagatgctg aaatgaccga caacaacatt
     1561 cgtgttgaca accgggaggg gggactcagg gtccgtatga cccgtaatct tcgtgatctt
     1621 gccagtgcgg tcttcggaaa cggagataac attacaacga taactgacaa cggtatgtcg
     1681 gtgacaaccg gtaccggaga taacgtgcgt accgtttctc ttggtaaaga ggggcttaat
     1741 aatggcggga atattatcac tcatgtggcc agcggactga acgggaaaca actgtctgaa
     1801 gctgacggcg acacactgac aaatgcagcc aatattggtg atctgaaatc agccctgcag
     1861 gacacaacgt cagcactggt gaacagtggt tacggagtgc aggctgatga cggaaacaaa
     1921 gtcacccgtc cactgggaga acatgtcagt attgccggtg atggccgtaa catcacgacc
     1981 atggttcagg acggaaaact gctggttcag ctttcagaac atattgatac cggtaaaaat
     2041 ggcagtgtga cagtgggcga tacggtgata acagatgaag gactgagcat tacaggcgga
     2101 ccatctgtca caaaaagcgg gattaatgca ggcgatcagg ttatttctgg tgtacaggat
     2161 ggcgttgatg ctcatgatgc cgtcaatgta tctcagttaa accagcaaat taataatgcc
     2221 actgctgcaa caacctggtc tctgaaaacg gaaagcagcg acgcctctgt tgcagtatcg
     2281 tctcagactg tggaagtcaa acatggcctg aacacacgtg tatcggctgt acagactgat
     2341 gacaaaggga attacagcta tgaaattgat gtgaccggta ttcccgtgga atataccgac
     2401 agcaaaggca acccgctggt gaatattggc ggcaagtttt ataccaggac tgaagatcct
     2461 gacagtggca acatcaccct ttctccggca gagccggcca gggttcgtat cagcagtgat
     2521 cagcccttgg tcctgacaag cgtggcggac ggtgcagtaa ctccggagtc tgtggatgcg
     2581 gtaaacggag gacaactggc gtcagcaggg aatattgttg ccggcaaaaa tgtggtatgg
     2641 aataacggaa aagcgaccct ttcaccggat acgttcagtg aactgaccac tgccgacgga
     2701 agcaaccgta ccacgacagg taccatacag actgtaccgg ataatgtggc agatgctgtc
     2761 aatatgctga accgtgaagg gacaaaatat ttcaaggcat cctcatcagg accggcggca
     2821 aaagcagaag gcaggaacag cgtggccata ggacaaagtg ccatttcccg tgctgaaaac
     2881 agcattgcaa tggggaatgg tgcagaagtc agtggtgatg cggctgccgg ctctgttgcc
     2941 attggcagtg gctcacgggc cggacgggca cacaccggta attattcgct gaatggtcag
     3001 gttgtggccg ggcgtacagg aagtaatacc ccggtcatgt cattcgggca ggccggtcag
     3061 gaacgccagc ttcagcatgt tgcaccaggg gtattatccg cagaatcaac agatgccgtg
     3121 aacggaagcc agttatatgc cgttcaccgc cagactgaag ggaacactca ggcaatcaat
     3181 aatctcggga agaaatttga tcatctgaat gacagagtgg atgagctgaa ccgggatatt
     3241 cgcggagtcg gcgcatcagc agctgcgatg agcggtattc ctcaggctta tctgccgggg
     3301 aaaagtctga tggggcttgg tgttggtggg tacggtggtg aaagtgccgt ggcaataggg
     3361 gtatcccgta tatcagataa cgggaaactt attatgaaaa tgaatgccgg gcaaaattca
     3421 cgcggaaact tttctgtggg agcaggtgtg ggctggcagt ggt
//
LOCUS       DAPPNJ010000300         3439 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000300 DAPPNJ010000000
VERSION     DAPPNJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.302"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..116)
                     /locus_tag="RPM73_004306"
     CDS             complement(<1..116)
                     /locus_tag="RPM73_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDX6522571.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTA"
     gene            complement(204..446)
                     /locus_tag="RPM73_004307"
                     /pseudo
     CDS             complement(204..446)
                     /locus_tag="RPM73_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(671..1774)
                     /locus_tag="RPM73_004308"
     CDS             complement(671..1774)
                     /locus_tag="RPM73_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDX6522572.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(1778..2485)
                     /locus_tag="RPM73_004309"
     CDS             complement(1778..2485)
                     /locus_tag="RPM73_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDX6522573.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(2492..>3439)
                     /locus_tag="RPM73_004310"
     CDS             complement(2492..>3439)
                     /locus_tag="RPM73_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522574.1"
                     /translation="SVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEEC
                     KEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKS
                     GAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSET
                     LTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSS
                     NADNMFDSIQTSFDKAGAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLG
                     NALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
BASE COUNT          936 a          719 c          645 g         1139 t
ORIGIN      
        1 gcggtcatat tctgccatgg cagaatctgc tccatgcggg agaggaaaat ctcttttcgg
       61 gtctgacggc gcttagtgct gaattcacta tcggcgaagg tgagttgatg gctcatgatg
      121 tccctctggg atgcgctccg gatgaatatg atgatctcat atcaggaact tgttcgcacc
      181 ttcccaagcc tgcaagtgaa gatccattgc tgtgttgagg gatcaaaaga caacaaaaac
      241 ttattgaaga aataatcttc tgcttttttg tcaaaataat gggcattgat cttgtctata
      301 gctgctgaat cttttccagc ctcgttgaga tcttcaaaat agccattgaa gatgttctgg
      361 ctaacaccgc cagtaacata agccttgccg ttgtgtacaa aagtcacatt acccgccatg
      421 cccatcggcg cgtgcgacat caatttattg ctttattcat tgtgactgtc ttctgtctac
      481 caagctttaa ccttcaccca tatgagggaa gttttaagta tgaggcgaac catagcatat
      541 gcttttatac ctgttacacc ataaaaatca gacaagttat gccataaact acttttgtaa
      601 tttgagtcac aaatcaggga aactttattt atcttccgtg aaagccttaa ctttcacgga
      661 gttttagaat ttacaatttt gctggcgtaa aggctccggt ttgtatttct ttaaccagct
      721 cacccacggt atatattctc catccttcga cttcggcggg agtaaggtta actgcaattt
      781 taaggcctgg ccatgcagct tctacggttg caataatttc atgttctgag ttttgaagat
      841 ctaacccagg aacaggaggt tgaaagcctt tatgttgcat catattaata tcaccatgca
      901 ctgatggatc agtcaggtca tatatttcat tccaggattc tgcttttaat aacccttgtt
      961 gggaacgtaa acgttgaatg atagccatct ctgcttgggt aacgaatata ccttcgtgga
     1021 ggggctgttt tcgatggcaa tccttgcaca gaacaagaag gttttcatgg tgattatcat
     1081 atttgatgcc atttttatga tggacatggc acaagttttt ggcggttgat aaattcacgc
     1141 cacaatcatt acaaacataa tttgcctttt ctcgtatttc tttagatatt tccttccagt
     1201 tttcgacata cccactattt gttttgtcat agataccctt aggcatatga cgaaaacagg
     1261 tactataatc tgaaaaaaac tcatttaatg agaatgattt aaaaatctct ctttttcgtt
     1321 gtttatcaat acttccttta taattaagtc gttcgaggca attcatacat acccgtaaat
     1381 caacatcctg gctccgacct gaaccatcat caatttcgaa taaaccatct atgcggttag
     1441 ttgcatgata cctttcaaat cgatttttat ggcgcatttc atcaagagtt ctgcaccagg
     1501 caatatgaaa acgttttcct ttttcgccat ctacgatagc cgcatcataa cgccctgaat
     1561 ggtcccgaat atataaaaga acctggcggc catgataaga gataagcccg ctaccagggt
     1621 caagatcttc gatactaact tctttacccg tggataactc aatatctata ggatcaaagt
     1681 caatagggga ttcatccaga aaaaaatcga ctccttctgg taacattttc ttccctaatt
     1741 gaataagttc tgaaatgtcg atagataatt tcatctctta ctcctgaata atctttctaa
     1801 tctgcaactc ggcatttgtt acaactttga aggtgacacg acgagagcga ttagggtcct
     1861 ctttgccggt tttatcaaga atgggatgtg cagatgaata acctactgcg gcaaatttac
     1921 ttttaaccca ttgttggtgt gtcgcgatat tttttatgtc ataaacgtat tgtaatactg
     1981 cacgtgtacg accttgcgat agtgccatat tattaaaata agcaatatca ggattcgttg
     2041 ttcctgtcca gtcagtactt gtgtgacctt caatgcggac ttcagtaata tgttcctgat
     2101 aattatctag aacttttagg tagcgaggaa agaagtcgtc aagaatgagt ttaaactttg
     2161 gttttagctc tgtgcttcct aagccaaata aaacatccgg tgatttaaat cgaacctcca
     2221 gagtctgttt atcgatctct gcatcccagt cttgtaaatc ctttgcaaac tcaatatcca
     2281 gagcattata aagctgtaac tgagcattct cgtaggctac ggcaacttct ttaatttttt
     2341 ctttttcaat acgtacgtag tgcatataag caatagatat gaacataaaa accatcatca
     2401 gccctgccat taggtctgac atggataccc aatgttcatt atcttgatct tttttaggta
     2461 attgtttccc tataatctta tccatgatcg attaccctcg atgtttatta gcagaaatta
     2521 atgcttgtaa ctgccccata actatttggt aatctttaat aagtttttca tagttaccga
     2581 caaaaccttt gctgattgaa agcagagcat tacctaatga ttgcatttca cggttcattt
     2641 cacgttcagt tgcttgctca aaagcatgta actgctcgtt gatggataga gcaaattttt
     2701 caattgattc tctgacttga gaagtcagcc ctgcaccagc cttatcaaat gatgtctgga
     2761 ttgaatcaaa catattatcg gcattactgg atagttgatg gttaatcttt tcaccagtct
     2821 tactgaacat atcaatcaca ttcgtcgtat tttgttccat atttctatgc aattctttcg
     2881 agtgcgactc tagtgatttc aggaattctt caccactttg tttcatttca gtaattgttt
     2941 cacctaacgt gctggttagg gtttcggagg aattgctgac atcatgcgcc atcgaggcaa
     3001 atgcttgttg tgtttgggta accgattgtc tgaatccttc ggtaccttca tccagggcaa
     3061 cgcgcattga atctgcatta agaagtattt gctggctggt ttgctcaaga gatgcactaa
     3121 catttgcagc tccggatttc agcagttcac ccacttcagc cattttgttc tgtatttcag
     3181 gtaatacggt tgtagcttta tcgcggatgg cgacaaaggt ttctaaatgg cgggagagtt
     3241 cgctgatttg atgttggttc acctgaagca cttcacgcag ttcagacata gccagaggaa
     3301 tttctttaca ttcttcccaa atccctgcaa ccgcagtttt tgtttcaacc agggactcga
     3361 cactttgttg atattgttct gacatctgct caatttgcgt tttataattt ccctgccact
     3421 caacaagttt ttttacaga
//
LOCUS       DAPPNJ010000301         3439 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000301 DAPPNJ010000000
VERSION     DAPPNJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.303"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            193..1158
                     /gene="dusB"
                     /locus_tag="RPM73_004311"
     CDS             193..1158
                     /gene="dusB"
                     /locus_tag="RPM73_004311"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDX6522575.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1184..1480
                     /gene="fis"
                     /locus_tag="RPM73_004312"
     CDS             1184..1480
                     /gene="fis"
                     /locus_tag="RPM73_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDX6522576.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1566..2450
                     /gene="yhdJ"
                     /locus_tag="RPM73_004313"
     CDS             1566..2450
                     /gene="yhdJ"
                     /locus_tag="RPM73_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDX6522577.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASPYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2534..2713
                     /locus_tag="RPM73_004314"
     CDS             2534..2713
                     /locus_tag="RPM73_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDX6522578.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2716..3378)
                     /gene="acrS"
                     /locus_tag="RPM73_004315"
     CDS             complement(2716..3378)
                     /gene="acrS"
                     /locus_tag="RPM73_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDX6522579.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          941 a          789 c          812 g          897 t
ORIGIN      
        1 ttctttgagt gtaaatttta gtcactattt tctaatatga tgatttttat gagtaattat
       61 cgcaccacgc tcattttaaa tgcaattctt tgatccatct cagaggattg gtcaaagttt
      121 ggcctttcat ctcgtgcaaa aaatgcgtaa tatacgccgc cttgcagtca cagtatggtc
      181 atttcttaac tcatgcgcat cggacaatat cagctcagaa atcgcctgat cgcagcgccc
      241 atggctggca ttacagacag accttttcgg acgttgtgct acgagatggg agccggattg
      301 acagtatccg agatgatgtc ttctaaccca caggtttggg aaagcgacaa atctcgttta
      361 cggatggtgc acattgatga acccggtatt cgcaccgtgc aaattgctgg tagcgatccg
      421 aaagaaatgg cagatgcagc acgtattaac gtggaaagcg gtgcccagat tattgatatc
      481 aatatgggtt gcccggctaa aaaagtgaat cgcaagctcg caggttcagc cctcttgcag
      541 tacccggatg tcgttaaatc gatccttacc gaggtcgtca atgcagtgga cgttcctgtt
      601 accctgaaga ttcgcaccgg ctgggcaccg gaacaccgta actgcgaaga gattgcccaa
      661 ctggctgaag actgtggcat tcaggctctg accattcatg gccgtacacg cgcctgtttg
      721 ttcaatggag aagctgagta cgacagtatt cgggcagtta agcagaaagt ttccattccg
      781 gttatcgcga atggcgacat tactgacccg cttaaagcca gagctgtgct cgactataca
      841 ggggcggatg ccctgatgat aggccgcgca gctcagggaa gaccctggat ctttcgggaa
      901 atccagcatt atctggacac tggggagttg ctgcccccgc tgcctttggc agaggttaag
      961 cgcttgcttt gcgcgcacgt tcgggaactg catgactttt atggtccggc aaaagggtac
     1021 cgaattgcac gtaaacacgt ttcctggtat ctccaggaac acgctccaaa tgaccagttt
     1081 cggcgcacat tcaacgccat tgaggatgcc agcgaacagc tggaggcgtt ggaggcatac
     1141 ttcgaaaatt ttgcgtaaac agaaataaag agctgacaga actatgttcg aacaacgcgt
     1201 aaattctgac gtactgaccg tttctaccgt taactctcag gatcaggtaa cccaaaaacc
     1261 cctgcgtgac tcggttaaac aggcactgaa gaactatttt gctcaactga atggtcagga
     1321 tgtgaatgac ctctatgagc tggtactggc tgaagtagaa cagcccctgt tggacatggt
     1381 gatgcaatac acccgtggta accagacccg tgctgcgctg atgatgggca tcaaccgtgg
     1441 tacgctgcgt aaaaaattga aaaaatacgg catgaactaa ttcaggttag ctaaatgctt
     1501 gattaaaaag gcgctactcg gcatggggaa gcgccttttt tataggtgtc acaaagggag
     1561 tgaccatgag aacaggatgt gaaccgaccc ggtttggtaa tgaagctaag accattattc
     1621 acggtgatgc gcttgccgaa cttaaaaaga tccccgccga aagtgtcgat ctgatctttg
     1681 ccgacccacc atataacatc ggtaaaaatt ttgatggtct gatcgaagcc tggaaagaag
     1741 atctgtttat cgactggctg tttgaagtga ttgcagagtg ccaccgcgtt ctgaaaaagc
     1801 agggcagcat gtacatcatg aacagcacgg aaaacatgcc ctttatcgat ctccagtgcc
     1861 gcaagctttt taccatcaaa agtcgcatcg tctggtcata tgacagttct ggagtacagg
     1921 cgaaaaaaca ctacggctcc atgtacgaac ccatcctgat gatggtgaaa gacgcaaaga
     1981 actacacatt caacggtgat gctattctgg tagaagccaa aaccggatcg cagcgcgcgt
     2041 tgatcgatta tcgcaaaaat cctccacagc catacaatca tcaaaaagta ccgggtaacg
     2101 tctgggattt tccgcgcgtg cgttatttga tggatgaata tgagaaccac ccgacgcaaa
     2161 aaccggaagc cttactgaaa cgcattattc tcgcctcttc caacccaggc gatatcgttc
     2221 tcgacccgtt tgccggtagc tttactaccg gtgccgtagc cgtctccagc gggcgaaaat
     2281 tcattggtat tgagatcaac agcgagtaca tcaaaatggg gcttcgacgg ctggatgtcg
     2341 cgtcgcctta ctctgcggaa gaactggcga aagtgaaaaa aagaaagacc ggcaacctgt
     2401 caaaacgaag ccggttaagc gaagttgacc ccgatctcat tgcaaagtaa aggaagtgta
     2461 agcctggttt tcagattatt catttcgtgt atatttcctg ccagacttgg ttaaacatgc
     2521 acaggctcag gtaatgattc gcaagtattg gtggctcgtc gttttcgctg tcttcgtttt
     2581 tctgtttgat actttactga tgcagtggat tgaactgctg acaacagaaa cagacaaatg
     2641 ccgcaatatg aactcagtta atccactaaa actggttaac tgtgacgaac tgaattttca
     2701 ggacagaatg tgaatttaca tgacacttaa ttcattcgtt tgatgaatta atttcgttat
     2761 gttttcatct ggcatgaaca ttcttaatac gttatcgacc agagcggggg cttgtttata
     2821 aagatcataa cccgccatat tcattaacca gttttgaaca attccgctga aggcaccatt
     2881 aataataatc atcacaacat ctaaatcgag gttatttgct acacaacctt gttgctgaca
     2941 cgcctgcaat acttcgcgga gagtctgcgg attgaagccc atcttttcgc gtatcactcc
     3001 ctcggccagc atctcatcat taaattcaca tttgtgatat aagattttca gcaacgcctg
     3061 ctggcgggga attttggcaa tatattgcaa gccgacaatc aatttttcac gcaattgttg
     3121 aaatgggtca tgctctaacc cagccgtcaa gtgatcctgg attaactctc gcaatgaagg
     3181 ctgttgcaac cacatctcat taaacagttg agtcttgttt tcgaagtgcc agtagatagc
     3241 gccacgcgta acgttagcag cgtcggcaat gtcgttgagt gtcgtcttgc ttacgccatg
     3301 ctgcgcaaac tgggcgatgg cagtttcaat cagttcttgc cgggtcttca gagcttcggc
     3361 tttggttctt tttgccatga ttaattattc aggaaataaa tatattcgac acagagtgag
     3421 aaaatagcga aggttaatc
//
LOCUS       DAPPNJ010000302         3409 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000302 DAPPNJ010000000
VERSION     DAPPNJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.304"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            36..743
                     /locus_tag="RPM73_004316"
     CDS             36..743
                     /locus_tag="RPM73_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDX6522580.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            740..2191
                     /gene="djlC"
                     /locus_tag="RPM73_004317"
     CDS             740..2191
                     /gene="djlC"
                     /locus_tag="RPM73_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDX6522581.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2251..>3409)
                     /gene="hscC"
                     /locus_tag="RPM73_004318"
     CDS             complement(2251..>3409)
                     /gene="hscC"
                     /locus_tag="RPM73_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDX6522582.1"
                     /translation="DLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVL
                     KRADVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWL
                     PLLNRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPS
                     TIVALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPV
                     SRVETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDI
                     NGLLEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAK
                     LEELWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
BASE COUNT          879 a          805 c          865 g          860 t
ORIGIN      
        1 gtagtcagtt aatgcactta agggattatt aacaaatgaa taaggaagaa caatatcttc
       61 tttttgcgct ttctgcgccc atggaaatac ttaatcaggg ttgcaaaccc gcgcatgact
      121 cgccaaaaat gtataccggt ataaaagaat tcgatctctc atcatcctgg ggaataaata
      181 atcgtgatga tttaattcag actatttatc agatgactga tgatggtcat gccaatgatt
      241 tagctggctt atatctgaca tggcatcgct cttcccctga agagtggaaa gcattaattg
      301 ctggtggctc ggagagaggg ttgatttata cccaattcgt tgcccaaacc gctatgtgct
      361 gtggagaagg gggaatcaaa gcctgggatt atgttcgaat ggggtttctg tcacgggtcg
      421 gtgtgctcaa caactggctg acggaagaag agagtttatg gctgcaatcg cgcgtctatg
      481 tacgagctca ccattactat cacagctgga tgcactattt ttccgcctat tcgttaggac
      541 gtctctactg gcaatcttct cagtgcgaag acaacacttc gctacgcgag gcgctaaccc
      601 tgtacaaata cgatagcgcc gggagtcgga tgttcgaaga gctggcagcg ggaagcgatc
      661 gtttctatgc cacgcttccc tggcagccat taactgtcca gcctgaatgc ccggtaacgc
      721 ttaaggatgt gagcgaccta tgaaaacatg ttggcaaatc ctcgaaattg aaagcacgac
      781 gcaaatagac attatccgcc aggcttatct tgctcgctta ccgttatgtc atcccgaaac
      841 cgatccgcag gggtttaaag cattacgcca ggcctatgaa gaggcgctgc gactggcggt
      901 aaatcctgtc gaggaagcag atgatgaaga aaaagatgcc gccgctgaac atgaaatact
      961 gcgtgcattc aggacattac tggattcaga aagtgatcgt tttcagccct ccgcctggca
     1021 gaaatttatt cagcaattaa atacctggaa catggaggat gtcgatcaat tacgctggcc
     1081 gctgtgtgca atcgctatag aagcgcgata tctttcatta aattgtgctt ctttgctggc
     1141 agagcgtttg aactggcatt cgtttaatga cagcgaagga atggatgagg aagaaaggga
     1201 ggcttttctt gaggccattc aggctggtga ttgtttcgat ttccttagcc ttctggaata
     1261 tcccgttgcg ttgcagaacc agactgttga gtattacttc gcgctggaac gttgctgccg
     1321 ttaccatcct gactacgtca ctgcgttttt ggcgatggaa ggtccgtggt tcattcctga
     1381 tgatgcaaag ttacatcgca aactgttgcg ctggtacagc tcggtgcaaa caggtatggc
     1441 ggaactcatt cctgtagcca aacagtggca aatggaagaa ccagaaagcg aagatgcccg
     1501 atattacttg tgtgctcaaa ggttgtactg cggcgagggg gaaagcctgc ttgccgatct
     1561 ctgcgcgtac cgggaaagtt acccatctac acaagctgat aatttgttgt tgcagtggag
     1621 caagagtcat tgcccggatt atttcgcgtt attagtgatg gttatcgaag cgcggagcat
     1681 ggtagatgcg caaggtcaac cgctgaaata tgttcctggt gagagcgccc ggacgcggct
     1741 gttatgggcg gaaattttac atagcggaaa attatcgccg ttaggtcaat cgtttattga
     1801 gtcgttattc ttcaaacgca aagcatgggc gtggtggaaa tcgagagtcg gtagcgagac
     1861 agaagaagat tcaccgttac tggatttgta tcgggtagcg gaacaggtag tacttgaagc
     1921 gtttccgaaa caagagatgc tggcccgtct taatacaagg ctggaaggcg gagatgctca
     1981 tccattagag gccattatca cccggatgct gttgacgaaa gtaaaactcg agccggagga
     2041 tgaagatgtc gatgagccaa cacctgaaaa ccatgaagaa aaaaatgatg agggtgaaaa
     2101 accacagagc attaccagca ttatcaaaat cagtttaacg gtgctggtta taggttatgc
     2161 tctcggcaaa atcgcgatgt tgtttagttg acgatgtgtt ttttgcgccg atgccggatg
     2221 cagcatttac gccgcatccg gcagccgtga ttatgaggtc tcaatggcta aataaacgct
     2281 cgcccgccgc ctgacctcat cgacgcgggc catatcgttt gactgcaacg ccgcatcaaa
     2341 atcggtgatc acccggccaa tctcttctcg ctcgtcaccc agcgcccgcg cccacaactc
     2401 ttccagtttg gccttaaagg tgcggttgat gagcatatcg cgcggataaa ttttcaatgc
     2461 ggataaccgc gtccgactct cttcaatctg ctgcgcgctc aatgttaccg ggctatgatt
     2521 aatgactctg gacttaacgc tgccatcttc cagaagcacg tcaacttcaa gcaagccgtt
     2581 gatatcataa ctaaagcgaa tatcaatcga ctgataagcc ccggttttct tcaacggcac
     2641 atcgaaggat tcaaccagaa tgttattttt aactttgtgg ttttctccct gatagacgtt
     2701 aaccgtaata gaatcctgtt ccgggtgcat ggtcgagtaa gtttctacac gcgacaccgg
     2761 tactgtggtg tttcgttcaa taattggcga gaaaatgccg gaaatgccct ggcggttaac
     2821 ttcaacgccc aacgagtaag ggcaaatatc agtgaggatc acctcttcaa tatcttcact
     2881 gcgtaagcgg caggcggcct ggattgctgc gcccagcgcg acaatggtgc tcgggtcgta
     2941 actttgatac ggtaatttgc caaacagacg cacggcgatt cgctgcacca gcggcatttg
     3001 tgacgcgccg ccaaccagta ccagactgtc gatttgactt ggcttcagac gcgcatcgcg
     3061 caacgcctgt tcaatcggca ctcgcaagcg attgagcagc ggcaaccaca aatcttccag
     3121 ttcattctca taaaattcgc attcccgtgc ttcatcctgg tactgccagc gaatgtgtaa
     3181 tggcgattga ttgctacatt ttgccgcttc cacacaggca tacaaggctg ccagttcact
     3241 ctcgttaagc gtggtcctgg tgacatccgc gcgctttaaa acgtcatcga ccaacatatg
     3301 ggtaaaatct tcgccaccga ggaagttgtc gccagcggag gcgtgaactt caatcaccgg
     3361 cgtggcgtac tcaagcaccg taacgtcaaa cgtgccgcca ccgagatca
//
LOCUS       DAPPNJ010000303         3395 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000303 DAPPNJ010000000
VERSION     DAPPNJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.305"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            47..1198
                     /gene="yiaY"
                     /locus_tag="RPM73_004319"
     CDS             47..1198
                     /gene="yiaY"
                     /locus_tag="RPM73_004319"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDX6522583.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1363..2901
                     /gene="aldB"
                     /locus_tag="RPM73_004320"
     CDS             1363..2901
                     /gene="aldB"
                     /locus_tag="RPM73_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDX6522584.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3120..3263)
                     /locus_tag="RPM73_004321"
     CDS             complement(3120..3263)
                     /locus_tag="RPM73_004321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522585.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIK
                     TRD"
BASE COUNT          842 a          833 c          908 g          812 t
ORIGIN      
        1 tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac
       61 tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat
      121 gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt taacgaaatt
      181 aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta
      241 tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa
      301 agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa
      361 aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg
      421 ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga
      481 aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg cgattgttga
      541 taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa
      601 gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc
      661 tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga
      721 aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta
      781 tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat
      841 ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttttgct
      901 gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg actgtgccgc
      961 tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag cctgcattaa
     1021 cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt
     1081 gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggttttac
     1141 taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag cgatgtaatc
     1201 atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc tgtaaccctt
     1261 gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt
     1321 cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct
     1381 tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc ccgctatgac
     1441 aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca gaatctgacg
     1501 ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg
     1561 gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc
     1621 gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca
     1681 gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg
     1741 gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt
     1801 gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag
     1861 attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg
     1921 gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta
     1981 atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt
     2041 ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc
     2101 tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat tccggtgacg
     2161 ctggagctgg gcggcaaatc gccaaatatc ttctttgctg atgtgatgga tgaagaagat
     2221 gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggtgaagtt
     2281 tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc
     2341 gccatccgcc gtgtcgaaag cattcgcagc ggtaacccac tcgacagcgt gacgcaaatg
     2401 ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa
     2461 aaagagggcg cagacgtgct caccggcggg cggcgcaagc tgctggaagg tgaactgaaa
     2521 gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag
     2581 gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg
     2641 gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat
     2701 ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac
     2761 gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc
     2821 cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat
     2881 aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg
     2941 cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac
     3001 acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg
     3061 cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt
     3121 taatctcgcg ttttgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg
     3181 aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg
     3241 gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa
     3301 acctcgtcct gttgaaaaac cagacagcac atttaagaaa gtatataaaa aacaatatgt
     3361 cgttatataa ataaggaaat tgcactgcat catgg
//
LOCUS       DAPPNJ010000304         3350 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000304 DAPPNJ010000000
VERSION     DAPPNJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.306"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1004..2512
                     /locus_tag="RPM73_004322"
     CDS             1004..2512
                     /locus_tag="RPM73_004322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522586.1"
                     /translation="MKPSIEQLLHIYGPMSSSALREKLIANGLTEVNARQRISRVGGD
                     VQRFTHFSLPKNEAFLYLTKDYNSRRYWIALLESHVNARTVYAHTFFAFHPYQGAIPT
                     YLFSKVSGSPDKLKGHIASSRLEAQLLKGSVLRREVDVVLGDCLVTYINNINGVTDTQ
                     HVRNQLLVEDILIKGVADWIRHNGLGSFNKVQVRNSQNMPSFSHHHWDICAPSYIMPL
                     ATVQKGEMSNGFIVADVIYNDLDSKSIQTYLTKVQRCRNMRNTRPFLAMLIAERFDPE
                     AFRLAKQAGVMATTVSNLLGQDISALLSSLLNTLNRAGAIAAANPEKINELFKGLTRI
                     EGAAINIRGVMFEMLVGHIVLKTQNVSTVDLNKQISTEAGKAEIDVIGFRGEAEIKCY
                     ECKGYEVKRLISVEMIEKWLERVQRIRKFYSQIHVYRDRKMTFSFWTSSDFEPEALEL
                     LLHQKQRNTKISLDWKNGRDILSIVKEDNLKSIADVLQEHYLRHPLAPDADM"
     gene            complement(2741..3295)
                     /locus_tag="RPM73_004323"
     CDS             complement(2741..3295)
                     /locus_tag="RPM73_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX6522587.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRKPKLTDEQWAQAGRLIAAGET
                     RQRVALIYDVGVSTLYRKFPVGRD"
BASE COUNT         1007 a          748 c          674 g          921 t
ORIGIN      
        1 ttaattgact caaaaagtaa tggaagcaac gggaatatca tatagaatat aatactacct
       61 gtaaaatcac tatattcttc acgccaacca attggctttc tgggaagttt attaggaaac
      121 tcagaggtat ctccactcat tgtaatttct ctctcagtaa tttgtgcaac ttttatcact
      181 gtcaaccatg aggtagcaag gattttgatg gcatatttta acaaaatatc cacttagagg
      241 tatgaacttc cgcttctggc acacagagct ctagagacag tggtgtaaaa tcatggagaa
      301 gcggtgggat gaggtgctaa tcttttcatg caaaaaatac gtaaaatcaa taacggctgg
      361 acatcattca atactcacac tatcgaaagt ccaccatcca gccgcagcaa gttcttgcat
      421 acgacgtgtc tgcgggttca actcccgcca gctccaccaa tcatgattgg acggtgcaga
      481 acaacaccaa taaaaacagg aagttagcag tctcagcagg acaccgacca gacggtgagg
      541 agacaaaaaa ggatacgcaa aggagccgcg gctcccaagt gacatgaaag cccgctgatg
      601 cgggcttttt cgtttttatt acctgcggca acacgctatc cctcttcgtg ttatgggatg
      661 gaattccttt ttaccttcat tggtactttc ggataccaga taacaccatt acctatttga
      721 ccaatggcag cgaagctggt ttaaataacc tctctagcaa acaagtacaa ctaagttaag
      781 tggccaaaaa aatcgcaata aaacgtgcaa ttacattaac taagtaggac gtttttaaca
      841 caaaaacatc gattaaaaaa cgcactgtga caaacactga aaaaacaagc atcacacaca
      901 ttttttattc atgtttatca atatgttaaa acataacctt actaatcttg aattcatcat
      961 ccgctaatgt agttatatta taaatagcgc agtgtgacac tcaatgaaac catccataga
     1021 acaacttcta catatctacg ggccgatgtc ctcatctgcc ttaagagaaa aactgatagc
     1081 caatggccta acagaagtta acgccagaca gcgaatctcc cgcgttggag gtgatgttca
     1141 acgttttact catttttctt tgcccaaaaa tgaagcattt ctctatctga caaaggacta
     1201 taactctcgt cgatattgga ttgcattgct tgaaagtcat gtaaatgcaa gaacagtcta
     1261 tgctcacacc tttttcgctt tccaccctta tcaaggggcg atcccgacat atctttttag
     1321 caaggtttca gggagtccgg acaaactgaa gggacacatt gcgtcaagcc gtcttgaagc
     1381 gcaattgctt aaaggttcag ttctgcgtag ggaagttgat gtcgttttgg gggattgtct
     1441 tgttacttac ataaataata ttaacggggt aacggatacc caacatgtcc gaaaccaatt
     1501 gctggttgaa gatattctca ttaaaggtgt agctgactgg attagacaca atggccttgg
     1561 cagctttaat aaggtccaag tccgtaactc tcagaatatg ccttcattta gccatcacca
     1621 ttgggacata tgtgctccgt catatattat gcctttagca acagtacaaa aaggtgaaat
     1681 gagtaatggc ttcattgttg cagatgtcat atacaatgat ttagatagca aaagcatcca
     1741 aacctatctc acgaaagtac agcgatgcag aaatatgcga aatactcgcc cgtttctagc
     1801 tatgctgatt gcagaacgtt ttgatccaga agcctttcgc cttgcgaaac aagctggtgt
     1861 aatggctaca acagtatcta acctacttgg tcaagacatt tcagcactac tgtccagttt
     1921 attaaatact cttaatcgtg ccggtgccat tgcggcagca aacccagaaa aaatcaacga
     1981 actatttaaa ggacttacga ggattgaagg cgccgcaatc aatataagag gcgtaatgtt
     2041 tgaaatgtta gttggtcaca tcgtactgaa aacccaaaat gtcagcactg ttgacttgaa
     2101 taaacaaatc agtaccgagg caggtaaggc tgaaatagat gtgattggtt ttagaggcga
     2161 agcagaaatt aagtgctacg aatgtaaagg ctatgaagtt aagcgactaa tttcagtcga
     2221 aatgatcgaa aagtggctcg aacgagttca acgcatcaga aaattctaca gtcagattca
     2281 tgtgtaccgt gaccgaaaaa tgactttttc attctggacc tcatcagatt ttgaacctga
     2341 agcacttgag ctgctgcttc atcagaagca aagaaataca aaaatatcat tagactggaa
     2401 aaatggacgt gacattcttt ccattgttaa ggaagacaat ctgaaatcaa ttgcagatgt
     2461 tcttcaggaa cattatcttc gacacccact ggctcctgat gcagatatgt aatgtggata
     2521 agcctaaggt ttatttagct gcctttgatg ctgtctatta ccgaattgtg atggtctgcc
     2581 cagtacaaaa atatcattct gggcgaattg atttagaggg ttcaccggct ctaagaggcc
     2641 gatatgtggc tagtgtctga gttcccaaca gctcgacagt ttgggctgta aagttgtgct
     2701 gtctgccagc atcaatcttt accggtggcc tgcatcatgt ctaatccctg cctacaggaa
     2761 attttcggta tagtgttgac actcctacat cataaattaa tgctacgcgc tgacgcgttt
     2821 ctcctgcagc gattagtctc cctgcctgcg cccactgctc atctgttagt tttggcttac
     2881 gtccaccaat tcgtccctgt gcgcgagcag cttccagtcc agcttttgtt cgttcaacaa
     2941 tcagttcacg ctccatttca gccagggcac ccatcacatg aaagaaaaag cgccccattg
     3001 gggtactagt atcaattgaa tccgtcagac tacgaaagtt gatgcctcgt tcgcgcaact
     3061 cctcaaccag aatgaccaga tgccgcatac tacgccccag ccgatccagc ttccagacaa
     3121 ccagagtgtc acctgccgat aatgtcctga gcagtttttt cagtccaggc ctttcggact
     3181 ttgtaccgct tatcttgtct tcaaaaatca gctcgcatcc tgcacagttc agcgcattac
     3241 gttgtagatc tgtgttctgg tcatttgttg acacacgtac atagccaata agcatggtag
     3301 atctccctag caaaagcagg aatgatgcca tttgctcgtt atttctgcat
//
LOCUS       DAPPNJ010000305         3291 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000305 DAPPNJ010000000
VERSION     DAPPNJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.307"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>3291
                     /locus_tag="RPM73_004324"
     CDS             <1..>3291
                     /locus_tag="RPM73_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX6522588.1"
                     /translation="LSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTG
                     VVDGFGRTLTFHRAAEGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSS
                     PAGPRSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTA
                     GGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGL
                     DYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQ
                     TDAAGRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEK
                     GRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTT
                     RYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATISP
                     DGKRSTIAYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTE
                     QRGFDGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHG
                     WLTTLSHTCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLAT
                     RQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQA
                     TAYTLTGQLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTA
                     ANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTD
                     RIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRR
                     ERDLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQ
                     AKARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQM
                     AAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQ
                     SLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSL
                     GLKFHVNG"
BASE COUNT          712 a          890 c         1191 g          498 t
ORIGIN      
        1 cctgagtccg catgtgtacc tggcgacaaa cagccttcag gggccgtggt ggatactcag
       61 ctggccggag cgggtgccgg gggcggacga ggtgctgccg ccggagccgc ccgcataccg
      121 ggtgctgacg ggcgtggtgg acggcttcgg gcggacgctg actttccacc gggcggcgga
      181 gggtgacgtg gcgggcgcgg tgacgggggt gacggacggc gcgggccgcc gttttcacct
      241 ggcgctgacc acgcaggcgc agcgggcgga agccttccgt aaacagcgcg ccacgtcttt
      301 atcttcccct gccggtcccc gttctgcttc ctcttctctg gttttccccg acacgctgcc
      361 cgccggtaca ggatacggca cggataacgg tatccggctg gaggcggtat ggctgacaca
      421 cgacccggca tacccggatg aacagccgac tgcgccgctg gcgcgctaca cgtacacggc
      481 cggtggtgaa ctgcgggcgg tgtatgaccg cagcggaacg caggtgcgcg ggtttactta
      541 tgatgcggag cacgccgggc ggatggtggc gcaccattat gcggggaggc cggagagctg
      601 ctaccggtat gatgataccg gtcgggtgac ggagcaggtc aacccggagg ggctggacta
      661 ccgctttgag tacggagaga gccgggtaat catcacggac agcctgaacc ggcgggaggt
      721 gctgtacacg gaaggcgagg gtggcctgaa gcgggtggtg aagaaggaac atgcggacgg
      781 gagcattacc cgcagcgagt atgatgaggc ggggaggctg aaggcacaga cggatgcggc
      841 ggggcggcgg acggagtaca gcctgcatat ggcgtcgggt gcggtgacgg cggtgacggg
      901 gccggacggc aggacggtac ggtatggcta taacagccag cggcaggtga cgtcggtgac
      961 gtacccggac gggcttcgca gcagccggga gtatgatgag aagggaaggc tgacggcgga
     1021 gacctcgcgc agcggtgaga caacgcggta cagctatgat gacccggcga gtgagctgcc
     1081 gacagggata caggacgcga cgggcagtac aaaacagatg gcatggagcc gttacggtca
     1141 actgctggcc ttcacggact gctcggggta cacgacgcgg tatgagtacg accggtacgg
     1201 tcaacaaatc gccgttcacc gggaggaagg catcagcact tacagcagtt ataacccgcg
     1261 tgggcaactg gtcagtcaga aggatgcgca ggggcgtgaa atccgttatg agtacagcgc
     1321 cgcaggcgac ctgacagcaa cgatatcacc ggacggtaaa cgcagcacca tcgcatatga
     1381 taaacgcggg cgtccggtga gtgtgacgga aggcggtctt acgcgcagca tggggtatga
     1441 cgctgccggg cgcatcaccg tgctgaccaa cgagaacggc agccagtcca cgttccggta
     1501 tgacccggtg gacaggctga ctgaacagcg cggttttgac ggccggacgc aacgttacca
     1561 ctatgacctg accggaaaac tcacgcagag tgaagacgag gggcttatca ccctctggca
     1621 ctacgatgcg tcggaccgca tcacgcaccg gacggtgaac ggcgacccgg cagagcagtg
     1681 gcagtatgat gagcacgggt ggttaaccac cctcagccat acctgtgaag gccaccgcgt
     1741 gtcggtccac tacggctatg acgataaagg ccgcctgacg ggtgaacggc agacggtgga
     1801 gaacccggag acaggggaga tgctgtggga gcatgagacg gggcatgcgt acagcgaaca
     1861 ggggctggcg acccgtcagg agccggacgg tctgccgccg gtggagtggc tgacgtatgg
     1921 cagcggttat cttgcgggga tgaagctggg cggaacgccg ctggtcgagt acacgcggga
     1981 ccggctgcac cgcgagacgg cccgtagctt cggcggggcg ggcagtacag cggggtacga
     2041 acaggccacg gcgtacaccc tcacagggca gctacagagc aggcacctga accttccgca
     2101 gcttgactgt gactatacgt ggaacgataa cggacaactg gtgcgcatca gcggcccgca
     2161 ggagtgccgg gagtaccgtt acagcggcac gggaaggctg acgggcgtcc acactacggc
     2221 agcgaacctg gatatcgata tcccgtatgc gacggacccg gcaggaaacc ggctgccgga
     2281 cccggaactg cacccggaca gcaccctcac ggcgtggccg gataaccgca tcgcggaaga
     2341 tgcgcactat gtctaccgct acgatgaata cggcaggctg gcggagaaga cggaccgcat
     2401 cccggaaggg gtgatacgga tgcacgacga gcggacccac cactaccact acgacagcca
     2461 gcaccgtctg gtgttctaca cgcggataca gcacggtgag ccgcaggtgg agagccgcta
     2521 cctctatgac ccgctgggcc gccggacggg aaaacgggtg tggcggcggg agcgtgacct
     2581 gacggggtgg atgtcgctgt cgcgtaaacc ggaggagacc tggtacgggt gggacggtga
     2641 caggctgacc actgtacaga cccaacagac aagaatccag acggtatacc agccgggaag
     2701 cttcacgccg ctcctgagaa tcgaaacaga gaatggtgaa caggcgaagg cgcggcaccg
     2761 tagcctggcg gaggtgttgc aggaggacac gggtgtgacg ctaccggcgg agctggcggt
     2821 gatgctgggg aggctggagc gggaactgcg gcagggcagc gtgagtgaag aaagccagca
     2881 gtggcttgcg cagtgcgggc tgacggcgga acagatggcc gcgcaactgg aggcggaata
     2941 catcccggag aggaaacttc atctttacca ctgtgaccac cgtgggctgc cgctggcgct
     3001 tatcagcccg gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct
     3061 gggcgaggaa agcgcgcagc accttcagca gtcactccgt ctgccgggac agcagtatga
     3121 tgaggagtcg gggctgtact acaaccgcaa ccggtactat gatccgttgc aggggagata
     3181 tatcacccag gacccgatag ggctgagagg tgaatggaat ctgtataaat atccattaaa
     3241 ccctgttcgg tttatagatt ctttgggttt aaaattccat gttaacggtg a
//
LOCUS       DAPPNJ010000306         3257 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000306 DAPPNJ010000000
VERSION     DAPPNJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.308"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            272..1588
                     /gene="shiA"
                     /locus_tag="RPM73_004325"
     CDS             272..1588
                     /gene="shiA"
                     /locus_tag="RPM73_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDX6522589.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            1690..3144
                     /gene="amn"
                     /locus_tag="RPM73_004326"
     CDS             1690..3144
                     /gene="amn"
                     /locus_tag="RPM73_004326"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDX6522590.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          739 a          804 c          801 g          913 t
ORIGIN      
        1 tcagactcat cagggtgcca ttttgtgcat atgcggactt ttatgtttca tatctcgaac
       61 ctgtgggtcc tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcttcca
      121 gcgaattttt catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga
      181 ggtagtctca taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta
      241 attataaaaa gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc
      301 cgatgaaggg acgctttcgt taagtcgcgc ccgacgagct gcgttaggca gcttcgctgg
      361 tgccgtcgtc gactggtatg attttttact ctatggcatc accgccgcac tggtgtttaa
      421 tcgcgagttt ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgctacctt
      481 tggcgtcgga ttccttttcc gtccgctcgg cggtgtcatt ttcggtcact ttggcgaccg
      541 actgggacgt aagcgcatgt taatgctgac cgtctggatg atgggcatcg cgacagcctt
      601 gattggtatt cttccttcat tctcgaccat tgggtggtgg gcacctattt tactggtgac
      661 gctgcgtgcc attcagggat ttgccgtcgg cggcgaatgg ggaggcgcgg cgttgctttc
      721 cgttgaaagt gcaccaaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta
      781 cggtgtaggt ttactgcttt caactggact ggtttcattg atcagtatga tgacgactga
      841 cgaacagttt ttaagctggg gctggcgcat tcctttcctg tttagcatcg tactggtact
      901 gggagcattg tgggtgcgta atggcatgga ggagtccgcg gaatttgaac aacagcaaca
      961 taatcaagcg gccgcgaaaa aacgcattcc ggttatcgaa gcactattac ggcatcctgg
     1021 tgctttcctg aagattattg cgctacgact gtgcgagttg ctgacgatgt acatcgttac
     1081 cgccttcgca cttaattatt caacccagaa tatggggtta ccgcgcgaac ttttcctcaa
     1141 tattggtttg ctggtaggtg gattaagctg cctgacaatt ccctgttttg cctggcttgc
     1201 cgatcgtttt ggtcgccgca gggtttatat cacaggcgcg ttaatcggaa cgttgagcgc
     1261 atttcctttc tttatggcgc ttgaagcaca atccattttc tggatagttt tcttctccat
     1321 aatgctggca aacattgcgc atgacatggt ggtgtgtgtg caacaaccga tgtttaccga
     1381 aatgtttggt gccagttatc gctatagtgg cgctggagtc ggttatcagg ttgccagtgt
     1441 ggttggcggt ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa
     1501 ctggcatagc gtcgccattt atttgctggc tggatgcctg atttccgcaa tgaccgcttt
     1561 gttgatgaaa gacagtcaac gcgcttgata gcctggcgaa gatcatccga tcttcgcctt
     1621 acacttttgt ttcacatttc tgtgacatac tatcggatgt gcggtaattg tatggaacag
     1681 gagacacaca tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc
     1741 gacgcgctgt atgagcaatc tgtagtcgca ttacgcaacg ccattggcaa ctatattaca
     1801 agtggcgaat tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg
     1861 accgtaacct gggacggtag cacaaccaat ccccccaaaa cgcgcgcatt tggtcgcttt
     1921 acccacgcag gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctt
     1981 aatgaacaac ttacgttgct gtatcaggat tatggtgcgc atatctcagt gcaaccctcg
     2041 cagcatgaaa tcccctatcc ttatgtcatc gatggctctg aattgacact tgatcgctca
     2101 atgagcgctg ggttaactcg ctacttcccg acaacagaac tggcgcaaat tggcgatgaa
     2161 actgcagacg gcatttatca tccaactgaa ttctccccgc tatcgcattt tgatgcgcgc
     2221 cgcgtcgatt tttccctcgc gcggttgcgc cattataccg gtacgccagt tgaacatttt
     2281 cagccgttcg tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga
     2341 tgcagccaga tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac
     2401 tggatcaccg ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa
     2461 catcagatgc cagcatggca tttaattacc gccgatggtc agggtattac tctggtgaat
     2521 attggcgtgg gaccgtcaaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg
     2581 gatgtctggt tgatgattgg tcactgtggc ggattacgtg aaagtcaggc cattggcgat
     2641 tatgtacttg cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc
     2701 gatattccta ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc caccaagctg
     2761 gtgagcggca ggcccggtga ggaagtcaaa cagcggctac gtactggtac tgtggtaacc
     2821 acagatgaca ggaactggga attacgttac tcagcttctg cacttcgttt taacttaagc
     2881 cgggccgtag caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc
     2941 gtgccatacg ggacactact gtgtgtttca gataaaccgt tgcatggcga gattaaactt
     3001 cccggccagg ctaaccgttt ttatgaaggc gctatttccg aacatctgca aattggcatt
     3061 cgggcgatcg atttgctgcg cgcagaaggc gaccgactgc attcgcgtaa attacgaacc
     3121 tttaatgagc cgccgttccg ataataagtt ctgaaccttc ttatcagaaa tagatgtgaa
     3181 ggagtaagta agacacctgg caaatagcct gcaataatcg tgggctgttt gcttccttgg
     3241 gcggatacga gtttaat
//
LOCUS       DAPPNJ010000307         3233 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000307 DAPPNJ010000000
VERSION     DAPPNJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.309"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            148..1830
                     /locus_tag="RPM73_004327"
     CDS             148..1830
                     /locus_tag="RPM73_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDX6522591.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1882..3039
                     /locus_tag="RPM73_004328"
     CDS             1882..3039
                     /locus_tag="RPM73_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDX6522592.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3005..3127)
                     /locus_tag="RPM73_004329"
     CDS             complement(3005..3127)
                     /locus_tag="RPM73_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522593.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          987 a          694 c          645 g          907 t
ORIGIN      
        1 gaaagtaatg tcaacgcttt atggactgtt atttcccttt ttttagctaa atctgctatc
       61 tctttatgtg actaacttca cttacatcca cttatttctc ttcgtaaaat tacttcggaa
      121 ttaagtacaa taagaagagg aacatttatg aagtctgcat taaagaaaag tgtcgtaagt
      181 acctcgatat ctttgatact ggcatctggt atggctgcat ttgctgctca tgcggcagat
      241 gatgtaaagc tgaaagcaac caaaacaaac gttgctttct cagactttac gccgactgaa
      301 tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct tggttatgga
      361 caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg tgaagttgtc
      421 gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc aacgccgacg
      481 ctcctttcat taatggatga aggcgtacgg tttactaacg gctatgtggc acacggtgtt
      541 tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt tggtgtctat
      601 tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc tgaattattc
      661 cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa aatcagtaat
      721 gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac cacattttct
      781 gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt ccacgctgca
      841 ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt ccccgcaaaa
      901 ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg tgccaaaaca
      961 cttgaccagc catttatgct ttacctggct tataatgctc cgcacctgcc aaatgataat
     1021 cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc agataactac
     1081 tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca actgaagaaa
     1141 aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc ggttatcgat
     1201 ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta tcctggcggt
     1261 actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag
     1321 ctgatttccg caatggattt ctacccgaca gctcttgatg cagccgatat cagcattcca
     1381 aaagacctta agctggatgg cgtttccttg ctgccctggt tgcaagataa gaaacaaggc
     1441 gagccacata aaaatctgac ctggataacc tcttattctc actggtttga cgaggaaaat
     1501 attccattct gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat
     1561 aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg
     1621 cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa
     1681 aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga
     1741 gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat
     1801 atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga attttccgcc
     1861 gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt tcaatgtaat
     1921 ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg
     1981 atggatgaca gtactctgaa agagttcatc aaacaatata tcgcagcgtc tggcaatcag
     2041 gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa
     2101 gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg
     2161 aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga atttctggtt
     2221 ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt
     2281 aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag
     2341 tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat
     2401 tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg
     2461 ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg
     2521 cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat
     2581 gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg
     2641 catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga
     2701 gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa
     2761 tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg
     2821 aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa gcatcgtatt
     2881 actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat ccttttttca
     2941 accatggtac cttatatgaa cgccatggtg gagttagcta agaacagagt accgctttac
     3001 cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct tttaactcca
     3061 ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct gtcgggtgtt
     3121 tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt cagggcatta
     3181 gcaataccct tgattatcaa tagcataacc ataaccgatg aaaatgaaat att
//
LOCUS       DAPPNJ010000308         3213 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000308 DAPPNJ010000000
VERSION     DAPPNJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.310"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..558)
                     /locus_tag="RPM73_004330"
     CDS             complement(<1..558)
                     /locus_tag="RPM73_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDX6522594.1"
                     /translation="MNKIIIASLFSGVGFISLPSFATQDLSGFYLSGKVGGSAVHLAN
                     QSYSYKDLDYPQDNIKWSGGSNNDTVFGGGIAAGYNFYPQFSLPVRTELEFYARGKAS
                     SKYNLWRESAGAFSGREDIKNEITLNSLMLNAYYDFRNDSSFTPYVSAGLGYARIHHK
                     TTDVYDLKYYSSEETISDSRSDTDNN"
     gene            805..977
                     /locus_tag="RPM73_004331"
                     /pseudo
     CDS             805..977
                     /locus_tag="RPM73_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1116..1366)
                     /locus_tag="RPM73_004332"
                     /pseudo
     CDS             complement(1116..1366)
                     /locus_tag="RPM73_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1371..1550)
                     /gene="tnpB"
                     /locus_tag="RPM73_004333"
                     /pseudo
     CDS             complement(1371..1550)
                     /gene="tnpB"
                     /locus_tag="RPM73_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1650..2327
                     /locus_tag="RPM73_004334"
     CDS             1650..2327
                     /locus_tag="RPM73_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX6522595.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            2327..2674
                     /gene="tnpB"
                     /locus_tag="RPM73_004335"
     CDS             2327..2674
                     /gene="tnpB"
                     /locus_tag="RPM73_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX6522596.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            2694..>3213
                     /locus_tag="RPM73_004336"
     CDS             2694..>3213
                     /locus_tag="RPM73_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX6522597.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDH"
BASE COUNT          860 a          825 c          739 g          789 t
ORIGIN      
        1 attgttatca gtatctgaac gtgaatctga aattgtctcc tctgatgaat aatatttcaa
       61 atcataaaca tccgtggttt tatgatgaat tctggcatat ccaagaccag ccgaaacata
      121 tggggtgaat gatgaatcat tcctgaaatc ataataagca tttaacatca gagaatttaa
      181 tgttatttca ttcttaatat cttcccgacc tgaaaaggct ccggcagatt ctctccataa
      241 attatacttt gaagacgcct ttcctcttgc ataaaactca agctctgtcc gtactggtag
      301 tgaaaactgt ggataaaaat tatagcctgc agcaataccg ccaccaaaga cagtatcatt
      361 attacttcca ccactccatt ttatgttatc ctggggataa tccagatcct tatatgaata
      421 actctgattt gccagatgta cagctgaccc accgaccttt ccactaaggt aaaaaccact
      481 cagatcctga gtcgcgaatg atggcaatga aataaaacca acacctgaga ataaagacgc
      541 aataataatt ttattcatcc tgcaactcca ttaacatatt acttattatt acatcaatac
      601 tataaaccac aacacataga tatttacgtg ataaatatca cattacatgc aatcaacatt
      661 gtattattca tacgccacag gacgttatta atattttcca tgaaataaga ctacccaata
      721 actctcggtc tttcacagta atgcgaacaa acataaaatt cattattttt gtgtattgaa
      781 gtaatccaga tgtatttgat aactgtgttc ttcggcaact attacagtgg aaagcgtggt
      841 gtaccgtgca tggactgatc ggcacctgcc ggctgaacgg tatcgatccg gaaacgtatc
      901 tgcgccatat cctgagcgta ttgccggaat ggccttccaa ccgtgttgat ggtatgcttc
      961 ctagcatctc atcttgacac gacgattgta acctgatcag gcgacggagg tgaccctgat
     1021 cgggtgttga agtcctgtgc gggataaacg ccgacaggcc ttgaagtact gagtatggta
     1081 attctgccca ggaccacgtt tttgatggcc cattaacaca cattattatc tagcggcacc
     1141 gcgccatctt ccaggaagcg gctcagttcc acgcgatgag acagcgcata gacaatggct
     1201 ttgtgtaatg ccctgccagg ggaacatttc ggttactgct cctcgaacca cgaccacaga
     1261 tcttccagta gcggacgggc ataacgctgt tgccactggc ggatttttcc acgaggcggc
     1321 tactgatctt cttatcaaga cggtacaacc cggcgatttt cttcacggtt acacagccat
     1381 acatcatatt tatcccaacg gagaactttt atgcgggagc gggctttatt ggtgaagaca
     1441 aaagcagcac catcatgcca tgcagcgtta agttcgttct ggacgtactg tatcagtgtg
     1501 tcgatgcccc ggcgcatatt cttgcattgg caatcaccac catcccccat agatggttaa
     1561 gggtaagaat aaaaagggcg ctaacgcgcc ccgaaagtaa ccggctcatt taaaccgtct
     1621 ggtctgtttc ctccggctct acaaaaataa tgtccatcat ttttaatgga cactatcgta
     1681 tgaaacaccg gacctggatc actgaagctt tacgtcttca ctttgaagaa catttacccc
     1741 gggttgtggc cgggcgtcgc ctgggtgtac caaaatcaac agtttgtagt atgttcgtgc
     1801 gctttcggag agctggcctt tcgtggcctt tgcccgcagg catgtcggag caggaacttg
     1861 atgcctgcct ttacggacaa ttttccacgg taccagtcgt acgtcctgaa agcaccgtta
     1921 tatccgaagc ccccgtggta aaaaaacgtc cccggcggcc caacttccct tatgagttta
     1981 aaatcgcctt agtggagcag tcactgcagc ccggagcctg tgtggcgcag atcgcccggg
     2041 aaaacggaat caacgataac ctgctcttca actggcgcca tcaataccgg aaaggtggcc
     2101 tgctgccttc cggaaaaaat atgccggcac tgcttcccgt gacgttaacg ccggagccgg
     2161 ataataaaat cccggccccc gcacaggaac cagagcagat aaatacaccg tccgacagtc
     2221 tgtgttgtga gctggttctg ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg
     2281 cgttattaca gacacttatc cgcgaaataa aagggagcag ccactgatga tatctctccc
     2341 tgcaggttcg cgtatctggc tggttgcagg tatcaccgat atgcgaaatg gctttaacgg
     2401 cctggcatca aaagttcaga acgtcctgaa ggatgacccg ttctccggac acctgttcat
     2461 cttccgcgga cgccggggtg accagataaa agtgttgtgg gctgacagtg acggactgtg
     2521 cctcttcacc aaacgcctgg agcggggccg cttcgtctgg ccagtcaccc gtgacggcaa
     2581 ggtgcacctt actccggctc agttatccat gcttcttgaa ggtatcaact ggaagcaccc
     2641 gaaacgaacg gaacgcgctg gaatccgcat ataacccgtt gtaaagtgag gatatggaca
     2701 cctcacttgc tcatgagaac gcccgcctgc gggcactgtt gcagacgcaa caggacacca
     2761 tccgccagat ggctgaatac aaccgcctgc tctcacagcg ggtggcggct tatgcttccg
     2821 aaatcaaccg gctgaaggcg ctggttgcga aactgcaacg tatgcagttc ggtaaaagct
     2881 cagaaaaact tcgtgcaaaa accgaacggc agatacagga agcacaggag cgaatcagcg
     2941 cacttcagga agaaatggcg gaaacgctgg gtgagcaata tgacccggta ctgccatccg
     3001 ccctgcgcca gtcttcagcc cgtaaaccgt taccggcctc acttccccgt gaaacccggg
     3061 ttatccggcc ggaagaggaa tgctgtcctg cctgtggtgg tgaactcagt tctctgggat
     3121 gtgatgtgtc agagcaactg gagcttatca gcagcgcctt taaggttatc gaaacacaac
     3181 gtccgaaaca ggcctgttgc cggtgcgacc ata
//
LOCUS       DAPPNJ010000309         3168 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000309 DAPPNJ010000000
VERSION     DAPPNJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.311"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            150..1670
                     /locus_tag="RPM73_004337"
     CDS             150..1670
                     /locus_tag="RPM73_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDX6522598.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="RPM73_004338"
     CDS             complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="RPM73_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDX6522599.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2152..2991
                     /gene="hdfR"
                     /locus_tag="RPM73_004339"
     CDS             2152..2991
                     /gene="hdfR"
                     /locus_tag="RPM73_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDX6522600.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3087..3162)
                     /locus_tag="RPM73_004340"
     tRNA            complement(3087..3162)
                     /locus_tag="RPM73_004340"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3127..3129),aa:Trp,seq:cca)
BASE COUNT          854 a          772 c          779 g          763 t
ORIGIN      
        1 gtccgctgca tttttgcgag catcattcca ccgccagaac aactcaactt cacttgcaca
       61 aataaacagg aatcagccac taaaattcac gaattagcgg gctacagcag cgatatctta
      121 tgcacataat ggtgactcaa ggagggctta tgtcactgtc aattgttcat acccgcgcag
      181 ccctgggagt aaatgcgccc ccgatcactg ttgaggtaca tatcagtaaa ggtctacccg
      241 gcttaacgat ggtaggctta ccagaaacaa ctgtaaaaga agctcgcgat cgcgtgcgca
      301 gcgccattat caatagcgga tatgaatatc cggcgaaaaa aatcaccatc aaccttgctc
      361 cagccgatct gccaaaagaa gggggacgat atgatttacc tatcgccatt gcgttgctgg
      421 cggcctcaga acagcttaca gccaataagt tagatgaata tgaattagtc ggagaactgg
      481 ctcttacagg cgctctgcgt ggcgttcccg gtgcaatctc cagtgcaact gaagctatta
      541 agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg
      601 gtgaaggatg cctgatagcc gatcatctgc aggctgtctg tgcgtttctg gaaggtaagc
      661 acgctctcga acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa catgatctca
      721 gtgatgttgt cggtcaggag caaggaaagc gaggactgga aattaccgcc gctggcgggc
      781 acaacctttt actgattggg ccgccgggaa caggtaaaac aatgctcgcc agccgtatta
      841 atggcctgtt gccagattta agcaatgaag aggcactgga gagcgctgcg atattaagtc
      901 tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg cccgttccgc tcaccgcatc
      961 acagtgcgtc gttaactgcg atggtgggcg gtggtgcaat tccagggcca ggtgaaattt
     1021 cgttggcgca taacggcgtg ctttttcttg atgagctacc tgaatttgaa cggcgtacac
     1081 tggatgcctt gcgagagccg attgaatccg ggcagatcca tctttcacgc acacgagcca
     1141 aaataaccta tccagcccgt ttccagcttg ttgcggcgat gaatcccagc cctaccggac
     1201 attatcaggg aaaccataac cgctgcacgc cagaacagac attacgttat ctcaaccggc
     1261 tctcggggcc ctttctcgac cgcttcgatc tctcactgga gatcccatta ccaccccccg
     1321 gcattttgag taaaacggta gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa
     1381 tggccgccag agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc
     1441 cggaaatacg ccaattctgc aaacttgaga gcgaagatgc gatgtggttg gaaggaacac
     1501 tgatccatct ggggttatcg attcgtgcct ggcagcggtt attgaaagtt gcacgaacca
     1561 ttgctgatat tgatcagtct gacattatca cacgtcagca tttgcaggag gcagttagct
     1621 atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca
     1681 tttcgccctt tttattaatc atcagaatcg gtgtagtctt cagcaccttc aacctgcggt
     1741 ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac
     1801 cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt
     1861 ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca
     1921 tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt
     1981 ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac
     2041 gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt
     2101 gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg
     2161 gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa
     2221 tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg
     2281 ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa
     2341 ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgcaa ggaggtggcg
     2401 catacctcac gacataacga gttttctatc ggtgccagcg cctcattgtg ggaatgtatg
     2461 cttaatcagt ggctgggacg tttgtatcaa aatcaggatg cccatacagg cttacagttc
     2521 gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat
     2581 cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctgggatat
     2641 ttcactttag cgctttatac cagtgcccct tcaaaactaa agggagatct taattatctg
     2701 cgacttgagt gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa
     2761 gtgcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat
     2821 ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc
     2881 gatagcacaa cactttcacg gccgctttat gccatatggc tgcaaaatag cgataaaaat
     2941 acgttgattc gcgatctttt gaaaattaac gtgctggatg aagtgtatta atatgaatgg
     3001 ctggcaagga tgccggtaga aggatttact tcggagaggg ttatttcaga taaaaaaaat
     3061 ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc
     3121 cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattag
//
LOCUS       DAPPNJ010000310         3158 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000310 DAPPNJ010000000
VERSION     DAPPNJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.312"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1876)
                     /locus_tag="RPM73_004341"
     CDS             complement(<1..1876)
                     /locus_tag="RPM73_004341"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015188.1,HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX6522601.1"
                     /translation="MNRFSQIVLNESTGEVGSDLSTGQEGQQQVKIRQTVLAPLIASF
                     LLTPSSVLAENYSNETLPNSTTAGSVLNEGDIATNTTINIGGHQYVYSGGSATDTLIR
                     GGDQHIYSGGSATDTLISGGDQHVSSGGSAINTIINGLHYTDGSQYVSSGGNAINTII
                     NDKGKQYVYNGGNAISTTINSRGVQVVSNGGIATNTTINGGGQIVYSGGKVSVSRLNS
                     GHQYVYSGGSATDIIIDRFGNQIVYSGGRVSNTTINGGYQSVHGGHAISTTINSGGQQ
                     SVLNSGSVTSTTINNGGTLMIDSNSTGTDIVQNGGGAIITDTAATVSGSNTNGEFSVA
                     DGRASNMLLENGGSLTVEPGHSVKDTMVGSGGLLGVVQDILLLGNNTIADSGKIVSDE
                     ITLSITNNGHLHFLNNSAATYTFDLTGTGDLKVDGGNLMLSGTLSQDGGVSIVNSGTM
                     VMDDLQATADLTMQAGTSLSLEQNSTLAGRIVGDGAGAGNVTVRSSLWELKGDSTVGE
                     LTMDNGTVHFHSPQSENWHPTTLTAGSLSGNGTFRMNTDIAAHTGDLLNVKGNASGNF
                     VLNIRNTGQEPASADSPLRVVHTGSGDARFTLNGGKVDAGTWEYYLNKENTDWYLKAD
                     V"
     gene            complement(1869..3080)
                     /locus_tag="RPM73_004342"
     CDS             complement(1869..3080)
                     /locus_tag="RPM73_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDX6522602.1"
                     /translation="MKTSFIYPPEIPTQSGPKHICYDFNDGARVLLPDGKWHVRLIDD
                     DSGNILFQCDSGKGWVTSTKKYFVRFRIQVYQQGSTHPILDETLNLTDKPVLISFPTG
                     TLGDLLGWFPYAERFQKQHQCRLECAMAQNIIDLLASQYPQIHFSTPDKLQTVLPYAT
                     YRVGLYFGGDTDNQPVDFRQVGFHRNAGYILGVDPREEPVRLDLSSPRTIQEPYVCIA
                     TQSTCQAKYWNNGTGWSEVVAHLKSLGYRVLCIDQYAHHGEGLIWNHIPWGTEDFTGS
                     HPLQERVNLLRHASFFIGLSSGLSWLAWATRIPVVLISGFSLPGSEFYTPWRVFNSHG
                     CNGCWDDTSLNFDHKDFLWCPRHKDTPRQYECTRLITGRQVNGVIDRLHLALAEQNGS
                     VTRDKKGVTDE"
BASE COUNT          829 a          801 c          664 g          864 t
ORIGIN      
        1 tgacatcagc tttcagatac cagtcggtat tttctttatt cagatagtat tcccatgtac
       61 ctgcatcaac ctttccaccg ttcagtgtaa atctggcatc accactgcct gtatgaacaa
      121 ctcgcagcgg cgagtctgca gaagctggtt cctgtccggt atttctgata ttcagcacaa
      181 agttaccgct ggcatttccc tttacattca acaaatctcc ggtgtgagca gcaatatccg
      241 tattcatcct gaaagttccg ttcccagaca gacttccagc cgtcagagtt gtaggatgcc
      301 agttttctga ctgtggagaa tgaaaatgaa cagtgccatt atccattgta agttcgccaa
      361 cagtggagtc tcccttcagt tcccataagg aactccgcac agtcacattt cctgcaccag
      421 cgccatctcc cacaatacgt cctgccaatg tgctattctg ctccagactc aggcttgtgc
      481 cggcctgcat cgtaagatct gctgtagcct gcagatcatc cataaccata gtgccactgt
      541 tcacgatgga gacaccacca tcctgtgaca gagtaccact gagcatcagg ttgcccccat
      601 ccactttcag gtccccagtt cctgtcagat caaaagtata agttgctgca ctgttgttaa
      661 ggaaatgcaa atgtccgttg tttgtaattg aaagtgttat ctcatcactt acaatcttac
      721 cactgtctgc tattgtgtta ttgccaagta gcaagatgtc ttgaacgacc ccgagtagac
      781 caccactgcc taccatggta tctttaacag aatgtcctgg ctccacagtc agactaccac
      841 cgttctccaa cagcatattg ctggcacgac catcagcgac actgaactcg ccattagtat
      901 tggatccgct tacggttgcc gccgtatctg tgataatggc ccctccaccg ttctgaacta
      961 tgtctgttcc agtggaatta ctatcaatca tcaaagtccc accattgtta atggtggtag
     1021 atgtcacact gccactgttt agaacactct gttgaccacc actgttaatg gtggtagata
     1081 tggcatggcc tccgtgaaca gactggtacc ctccgttaat tgtcgtgttt gagacgcgac
     1141 caccagagta aacgatctgg tttccaaaac ggtcaatgat aatgtctgtg gcactgcctc
     1201 cactatagac atactggtgc ccactgttca gtctggaaac tgagactttg ccaccgctgt
     1261 aaacaatctg cccaccaccg ttaatggtag tgtttgtggc aatgcctccg ttggaaacaa
     1321 cttggactcc gcgactatta atagtggtag atattgcatt gccaccattg tagacatact
     1381 gcttcccttt atcgttaatt atagtgttta tagcattacc accgctggag acatactgac
     1441 taccatccgt gtaatggaga ccattgatga tagtattgat ggcgctgccg ccgctggaga
     1501 catgctggtc tccgccacta attaatgtgt ctgtagcact gcctccacta tagatatgtt
     1561 ggtctccgcc acgaattaat gtgtctgtag cactgcctcc actatagaca tactggtgcc
     1621 caccgatgtt aatggttgta ttcgttgcta tatcaccttc attcaatact gagccagcag
     1681 tagtgctgtt gggcagagtt tcattgctgt agttttctgc cagaacagat gatggcgtca
     1741 acaggaaact ggcaatcaat ggcgccagta cagtttgtct gatttttact tgttgttgac
     1801 cttcctgacc agtgctgagg tcactaccca cttcccctgt ggattcattc aatactatct
     1861 gggaaaacct attcatcagt tactcctttt ttatcacgtg tcaccgaacc attctgttct
     1921 gcaagtgcaa gatgaagtct gtcgatcaca ccattaacct gcctgccagt tatcagtcgg
     1981 gtgcattcat actgtcgtgg ggtatcttta tgcctcggac accagagaaa atccttatgg
     2041 tcaaaattca gtgatgtatc atcccagcaa ccgttacaac catgggaatt aaacacacgc
     2101 cacggggtat agaactcgct ccccggaaga ctgaaaccac ttatcagtac caccggaata
     2161 cgggttgccc acgccagcca tgacagacca ctggaaagac caataaaaaa actggcatgt
     2221 cgaagcagat taacccgctc ctgcaaaggg tgactgccag taaaatcctc agtaccccac
     2281 ggaatatggt tccatatcaa accttctccg tgatgagcat actggtcaat acacaatacc
     2341 cggtaaccca gagatttcag atgtgccacc acctcactcc agcctgtacc gttgttccag
     2401 tatttcgcct gacaggtgga ctgggtagca atacacacat aaggctcctg aatagttctt
     2461 ggtgatgaca agtcaagacg taccggttct tcacgaggat caaccccaag aatgtagcca
     2521 gcattacggt gaaaacctac ctgacggaaa tccactggct ggttgtctgt gtctccgccg
     2581 aaatacagcc caactcggta agtagcataa ggtaaaactg tctgtagttt atctggcgtg
     2641 gaaaaatgta tctgaggata ttgagatgcc aacaggtcga taatattctg tgccatcgcg
     2701 cattctaacc ggcattgatg ctgtttctga aaacgttcgg cgtaagggaa ccaaccgagt
     2761 aaatctccaa gagtaccagt gggaaatgaa atcagcacgg gcttatctgt caaattcagg
     2821 gtctcgtcca gtattggatg tgttgagcct tgttgatata cctgaatacg gaaacgcaca
     2881 aaatatttct ttgtgctggt gacccaccct tttccagaat cacactgaaa aagaatgtta
     2941 ccagaatcat catctatcag acgtacatgc catttaccat caggcagaag cacgcgggct
     3001 ccatcattaa agtcataaca aatatgctta ggtccgctct gggttggaat ttctggtggg
     3061 taaataaaag atgttttcat tttgtatctg gataactcgc agtaattaat ggatgcgggc
     3121 tcattaaaca taatgaattc aattgctgaa ctggctgc
//
LOCUS       DAPPNJ010000311         3066 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000311 DAPPNJ010000000
VERSION     DAPPNJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.313"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="RPM73_004343"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="RPM73_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDX6522603.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="RPM73_004344"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="RPM73_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDX6522604.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          718 a          826 c          777 g          745 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
       61 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc ccacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac agcgaccggc
      301 aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac cagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagccgt ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
      661 cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtactcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
      961 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     1141 aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttcag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     2461 tctggttcga tcctgcttta tctggctgag aaatttggct acttcctgcc gcaggatttg
     2521 gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta cgccatcaac
     2641 cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttattg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aacgtggtgt taggtggtgt gtatgatgcc gctgagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     3061 acgctg
//
LOCUS       DAPPNJ010000312         3018 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000312 DAPPNJ010000000
VERSION     DAPPNJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.314"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(1..2888)
                     /locus_tag="RPM73_004345"
                     /pseudo
     CDS             complement(1..2888)
                     /locus_tag="RPM73_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          549 a          966 c          793 g          710 t
ORIGIN      
        1 gcttctcgtc caattttcgc aaattgctta gcatgaatca cttcatgata cacctcatat
       61 aaggtagcat ttttcctaag ataaatattg cgaaggccat aatcaaaagc tgccctagca
      121 tttggtggca aatatttatc tgctttcctt attactttaa ttcctagctt ctccatttct
      181 ttagcaatat tgtttatttg actacgtgat aaacgaaccc catccaggaa acccgtatgc
      241 ccactaacac gacgaccatc gccaatatag tccatcaaac tgctgatttt agaacttgca
      301 gccaatccca gcggatcaac ccacccacaa ggattcggca cataccggta agtctgctcc
      361 ccgcccagca accccagcgg atcactcacc aggtacattc cggtttccgg ctcgtaatac
      421 cggaaccggt tatagcacag ccctgattcc gcatcctgcc actgtcctgc atacagcagg
      481 ccggggtcgg cttccgggtc cagttccccg cgcgggtccg ggtagccggt gtctgcgggc
      541 agactgagtg ccagccccca caggctggtc tgccccggtc gccagacggt tttaccttca
      601 ctgttaaaga gcatcagcgg gcggcccgtc aggtcgctca ccgcgtgatt cgtccgcgtc
      661 acccactgcg gggccacgga cgggtgcggc tgccgtaccc ggctgaactg ctggcttatc
      721 agctcaaagc cgttaaacac caggtgcctt acgctgtaca gtttatcatc gcggtattcc
      781 cggatttccg caatactgtc gccatcccac aggtacgtaa aacggatttt cttccggtcg
      841 cagcgttttt ccgtccgcct gccgctggcg tcgtagcggt attcccactg ctccccctgt
      901 gcgctgcaat acccggtcag ctggtccagg ctgtcccacc ggaaccgctc tgtttccggg
      961 cggtagccgt cacggtgttt tgtccggctg accatccggc ctgcggcgtc atagtcatac
     1021 tgcgtgtttc cggcctgttt cagccggtgg cctccggcat actggtcagt ctcttcactt
     1081 atccggtgcc tgcccgcaga ctgcgccttc aggtagccac aactgtcata gccataggac
     1141 tcgtcgcctt cgccatagcc cagcccggtg cctgaggctg tcaccgccgt gacctggccg
     1201 ttccggtcca gccggtactc ccgccgtgtt tcccgtccgt aatcttcccg gctggtgacg
     1261 cctgacacgt tacccgcgct gtcgtaacgg tactcccgta ccagtgacgg aaccagcccg
     1321 ctccatgtgg cttcttccgg aaaaaatccg gcgcgctggc tgacaggctg accggctgca
     1381 ttccagccct gttcacaggc aaaaccccgg ttactgtgcc ggagcacttc acgtcccgcc
     1441 tcatcacggc tgaacgtcag cgtctcatcc cccggcagaa tgacctccgt cagttctccg
     1501 gcggcgttgt aacggtaggt ggttttcagc agggattcat cgggatgtct gtcgcgttca
     1561 tcttccggtg gcaggataca gcggatgacg gtgtgttcat cggggtagtg gtattcaatg
     1621 gtccgtcggc cctgagtggt gacattcacc cgcccgcgtt cgtcatagcc ggtgcgctgt
     1681 gatatgccgt ccgggctgaa gatacccgtc agcctgccgg atttgtcata cgcatactgc
     1741 gttttgcctt ccggcgtgat gatttctgcc aggcggcctg cggcgtcccg gctgtagagg
     1801 atggtttcct ccagaccgtt gaccctggcg gtacagaggc catcggcgtc atagcggtaa
     1861 cgccagacca cgccgcccca gtcacgctct tccgtcaccc gtccacagtt gtccagaata
     1921 taacggtatg actcgccctg cggattgatg acttctgtca gttgcagcgt gtctttatcg
     1981 tagcggtact gccagcggtg gccttcaccg tccgttcgcg ccaccggcaa atcgaacggg
     2041 ccgtattcca tcgtccagac aacgccgttg ccgtccgtcc aggccagcag gttgccgtgg
     2101 cggtcgtagc tgaacccttc cgcgccggtt ccagcacgtt caatgccact cagtttatgg
     2161 tgctgctgcc actgtaaccg cactgactga ccgccggggc cgcgcatctt caccggcagg
     2221 tcgtgaccgc tgtattcggt gtggtgttca cggcccatca cgtcggtgag tgtttccggc
     2281 cggttgtggt ggtcgtactc tatgcccaga cgccgttcat cctggcggaa tatccccgac
     2341 agcagcccct gcggcgtgta cgccagccgg gtctcttcgc cctgtggcga caccagactt
     2401 accggcaggc cgcgttcatc acactgctgt gtccacgtct gcccggctgg gtcggtgatg
     2461 ttgctgagca ggccgcgctc atcatagcgg tactgccact ccgcgccgtc tgccgccgtg
     2521 taacggctta actgaccgtg tgcggcgttg tattcaaact tctccacccg cccggcaggg
     2581 gagtgacggg ccagcaggtg atggaattca tcccattcat acgtggtggt gctgccgtcc
     2641 ggcgcttctt cccgcagaat attgccgtca ggatcatagt aataacggtg aatgcccttg
     2701 ccgtccgtca cggtggtgca gccgtcacca tagtccagcg tggcgttgta atagccctcc
     2761 gccccggtga cattcacgca ccggccctgt tcatcgtagg tgaaacggct ccacgtctgg
     2821 tcgttatcgg actagcggat gatccggtcc gcagcgtcgt actcataaaa caggtggtag
     2881 tccagccgta ttcatcctag cagttaatta agcaccatcg gatcaaagac gagaatatca
     2941 tgtcccttta gcccacacgg ccacccccca agtttataaa gcttaaagac atcctcaagg
     3001 aatgaatctt taatataa
//
LOCUS       DAPPNJ010000313         2929 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000313 DAPPNJ010000000
VERSION     DAPPNJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.315"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(125..2386)
                     /gene="katE"
                     /locus_tag="RPM73_004346"
     CDS             complement(125..2386)
                     /gene="katE"
                     /locus_tag="RPM73_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDX6522605.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2569..2832
                     /gene="cedA"
                     /locus_tag="RPM73_004347"
     CDS             2569..2832
                     /gene="cedA"
                     /locus_tag="RPM73_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDX6522606.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          704 a          721 c          761 g          743 t
ORIGIN      
        1 aataaactgc aatacattga atttatacga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat aaacaaagcg cactatgtaa atcatttgag gcggcgcaat tgcgccgcct
      121 cccatcaggc aggaattttg tcaatcttag gaatgcgtga ccacacgcgg tgtgctgcca
      181 tcagcgttag cagttcatcc ataaaactac cgtcagcgct gtcagcttcc acaatccctt
      241 cttcaccctg gtcagcgacc ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg
      301 caatcggttt aaggtgtttg taggcttcca tcaggtagta gttggcatcg ccgttgtcag
      361 cgatatccgc gatattgccg caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac
      421 cggcaaaggt agcggctata ggcaacaccg taccgtcatc cgcagtcact tcacccattc
      481 gggagtagag cagtttggca tgaacgcctt tggccttcag ggccttgaga atggccagaa
      541 ggtctgccga tctcacttca tcattaagta aaatcgctac cacgcgacct ttcacatcac
      601 cgtcaggaat ggcgtacaaa cttaaggatg gatccttttt cagaccgttg acgtccggag
      661 gtggggtgat attcagctgg tcgtcagtca gttcgatacc gagatttttc gccaccgcct
      721 gggccagagt gagatcaata tgcgccagct ggtcaacaac gcgctcacga atatacggac
      781 gaacgacttt gcttaactca aaactgaaac catcgacaat atggcgctgc tcgaatggcg
      841 tctgacttag ccagaacaga cgcggatggg aataatattc gccaaacgat ggactgcgct
      901 cacgaacttt atttccttcc acgcgttcct gatatgattc aaaaccgccg cgtttcggtc
      961 ccggcggtgt ttcgcgcggc cagttatcgt tgatcgagtt cggttcgtaa ttcgccgggt
     1021 tagtgtcgat ccccatacga tgcatgccat cacgctggaa gttgtggtaa gggcaggtcg
     1081 gacggttaat cggaatctca tggaaattcg gcccaccaag acgactgatt tgtgtatcgg
     1141 tataggagaa caaacgtccc tgcaacagcg gatcgttggt gaagtccaga ccaggaacaa
     1201 tatgacctgg atggaatgct gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat
     1261 tgagcaccat tttgccgaca cgctgaacgg gcaccagttc ttccgggatc agtttggttg
     1321 gatcgagaag atcgaagtcg aacttgaatt catcttcttc aggaatcaac tggaagccca
     1381 gttcgtattc cggaaaatcg cctgcttcaa tggcttccca caactcgcgg cggtggaagt
     1441 ccgggtcacg tccggtgagt ttttgtgctt catcccaaac gagtgaggct ttacctgcca
     1501 gtggtttcca gtggaaacgt acaaacgttg ccttcccttc ggcattaatc aggcggaagg
     1561 tgtgaatacc gaagccttcc atggtgcggt aactgcgggg gatgccgcga tccgacatcg
     1621 cccacatcac gttgtgcaga gtttcaggtt gcagagaaac ataatcccag aaagtatcgt
     1681 gggcactttg cccttgtgga attgcccagt gcggttctgg ttttaccgca tgaacaaaat
     1741 cggggaattt atgcgcatcc tggataaaga agattggcgt gttattgcca acgaggtcaa
     1801 aaataccctc ttcagtatag aacttggtgg caaaaccacg gatatcacgc acggtatcgg
     1861 cagagccagc gccaccctga acggtagaga acctaacaaa tactggggtg attttgttcg
     1921 gatctgagag gaaatccgct ttggtgatat cgcttaagct tttatatggc tggaaataac
     1981 cgtgagctgc tgatccgcgt gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt
     2041 gggtgatttt ctcgcgcaga ataaaatctt ccagcagcgt tggaccacgg ttaccggcac
     2101 gcagtgagtt ttgatcatcg gcgatgcgca cgccctgatt agtggtcagc gcataatttt
     2161 cactgccttt gcgtacgtct tccagagaat taagtttttc gttacgtgta tcaggggctt
     2221 tcaggctccc aggggcggta ggttgtgcac ctggcggtgt tggttcagcc gctggacgat
     2281 gagatccgtc ctcaggtgcc agtgagtcca tccccggttt cgcttcgctg gaatcgtgta
     2341 gtggtgactg gtgctgatgt gggttctttt cgttatgttg cgacattgaa ctcgtctcct
     2401 taatttatta ctgaaagggc cgctgttttg gtcaaaaaac cctctaacta tagaccatca
     2461 gccagatccc gcttcggaga ctattctgga aacggtgtta tgaaatacac gcaaagtggg
     2521 acgtacaggg gctaaatcgg ctaaactaca gtttttctga tttttaatat gcgtttagtg
     2581 aagccagtaa tgaagaaacc gctccgccag caaaaccgcc agattattag ctatgtccca
     2641 cgcacggaac ccgcgccgcc agaacatgcg ataaagatgg attcgtttcg tgatgtctgg
     2701 atgctgcgtg gcaaatatgt tgcgttcgta ctgatgggag agtcatttct gcgctcaccg
     2761 gcgtttactg tgcctgaatc cgcccagcgt tgggcaaatc agatccgcca ggaaggggaa
     2821 gtgactgagt aaagattacg tacctcccgg ttttgccggg agttacatga cctgaaattc
     2881 tgtatttcat tgctacattc attcaaacca ccagtaacaa aaagctgcc
//
LOCUS       DAPPNJ010000314         2880 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000314 DAPPNJ010000000
VERSION     DAPPNJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.316"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(113..1294)
                     /locus_tag="RPM73_004348"
     CDS             complement(113..1294)
                     /locus_tag="RPM73_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDX6522607.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1549..2778
                     /locus_tag="RPM73_004349"
     CDS             1549..2778
                     /locus_tag="RPM73_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDX6522608.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          731 a          726 c          623 g          800 t
ORIGIN      
        1 tcatgagaat ggaaaaatca gggagcccgg aaaatgtatg tcgacttgcc agccatcatt
       61 attcaacaga ttaatgacgg cagatattta tcggtaagga ggaaatgcag agctattcgt
      121 cacgttcaat ggtcaatgcc accccctggc ccccgccgat acaaagcgtt gccagtcctt
      181 tgcgggcatt acgtttcacc atttcatgaa ccagagaaac caggattcgg caaccggaag
      241 cgcctatcgg gtgaccgagt gcgatcgcgc caccattgac attgacccga cgctcatccc
      301 actcaagcat cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa
      361 gatcaacatc agccaactgc cagcctacac gctccaggca acggcgggtc gcatacaccg
      421 gcgcaattcc catcaatgcc ggatctacac caacgctggc aaatgcgcga atgcgggcca
      481 gcacgggtaa attcaacgct cgtgctttgg cttcgctcat catcattacc gcagctgcgc
      541 catcgtttat ggatgatgca ttacccgctg tcacagaacc gagactatca aatgaaggat
      601 ttaaacgggc taaaccttct gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa
      661 ccaacgtctg accgttactt tgggtcatta ccgggacgat ctcatcttta aatcgtccgg
      721 cgtcaatcgc cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaattgac
      781 ggctgatgcc atattcgcga gccagatttt cggcggtgac accaatatga taatcattga
      841 aggcatccca taacccatca tgcacaagac tgtcaaccag ctggctattg ccaagctgtg
      901 caccggtgcg gctatcagtc agaacatgtg gtgcgcggct catgttttcc tggccaccgg
      961 cgatgacaat atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa
     1021 gtccggaacc gcagacgtca ttaatcgtga ttgcagaaac gctattaggc agaccacctt
     1081 taatagccga ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa
     1141 ttacttcatc caccgcatat gcaggaacgc cggtacgttc tattaacgct ttcacgacca
     1201 gactaccaag ttccacggcg gaatgacccg ctaacgcacc gcgaaagcag ccgataggtg
     1261 tccgtaacgc cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc
     1321 aatagtaaat gattgttaat tacagttatc ttaattgttt aaaaaaagtg atttttatca
     1381 caaaggaaat atgcctgagc agtagtcaga gtcataactg gcacgtaagg tttgcaacca
     1441 ctaacccacc aatagagggg tagatagggc gttaatctcc catacttaac ctggtttatg
     1501 gtaaattgcc ctccattttg tttaatttgt agatgatacg ttcagataat gtctaatatt
     1561 tggtctaaag aagaaactct gtggagtttc gcgctctacg gcacagccgt tggtgcaggc
     1621 acgctcttcc ttcctattca gctaggttcg gcaggggctg tggtcctgtt tattactgct
     1681 ctggtcgcct ggcctttaac atattggcca cataaagcct tatgccagtt catcctctca
     1741 tcaaaaacat cagcaggtga agggataacg ggcgcggtaa cacactacta tggcaagaag
     1801 attggtaatc tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat
     1861 gcagtggcaa ttaccaactc acttacggaa cagctggcaa agcatatggt tattgatctt
     1921 cgcatccgta tgttggtgag tctgggtgtt gtattaattc tgaatctcat ttttctgatg
     1981 ggacgtcatg ccactattcg agtaatggga tttttggtat tcccattgat tgcctatttc
     2041 ttatttcttt ccatttacct tgtaggtagt tggcaacctg atctattaac aacccaggta
     2101 gagttcaatc agaataccct tcaccagata tggatatcga ttcccgtgat ggttttcgcc
     2161 tttagccata cgcccattat ttctacgttt gccatagaca gacgtgaaaa atatggcgaa
     2221 cacgctatgg ataaatgcaa aaaaattatg aaagtcgctt atctcatcat ctgcataagt
     2281 gtactgttct ttgtctttag ctgcctgctt tctattccac cttcgtatat tgaagcggct
     2341 aaagaagaag gggtcaccat tttatcggcg ctttctatgc tgccgaacgc ccctgcatgg
     2401 ttgtcaattt ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg
     2461 tactttggcg ttattgaagg tgccacggag gtcgtcaaaa caacactaca gcaggttggt
     2521 gtaaagaaaa gtcgtgcatt taaccgcgca ctatcaatta tgttggtatc gctgattacc
     2581 ttcattgttt gttgcattaa cccgaacgcg atttcgatga tttatgcgat cagcggcccg
     2641 ctcattgcca tgatactttt catcatgcct acgctatcaa catatctcat cccggcgctt
     2701 aaaccctggc gctccatcgg aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg
     2761 gtaatgttct ttagctaata tccattgtga atggttcaaa attgctacaa ttttgaacca
     2821 ttccagtttt cataatgtgc atttgttatt attccgctct atcacttctc tcgtcatatc
//
LOCUS       DAPPNJ010000315         2875 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000315 DAPPNJ010000000
VERSION     DAPPNJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.317"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..473
                     /gene="agaB"
                     /locus_tag="RPM73_004350"
     CDS             <1..473
                     /gene="agaB"
                     /locus_tag="RPM73_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDX6522609.1"
                     /translation="SPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQ
                     QKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKD
                     INVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            512..1315
                     /gene="agaC"
                     /locus_tag="RPM73_004351"
     CDS             512..1315
                     /gene="agaC"
                     /locus_tag="RPM73_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDX6522610.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1305..2096
                     /gene="agaD"
                     /locus_tag="RPM73_004352"
     CDS             1305..2096
                     /gene="agaD"
                     /locus_tag="RPM73_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDX6522611.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2097..2852
                     /locus_tag="RPM73_004353"
     CDS             2097..2852
                     /locus_tag="RPM73_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDX6522612.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          703 a          679 c          723 g          770 t
ORIGIN      
        1 gcagtccaaa tattctctta acccgtattg ataaccgtct ggttcacggt caggttggcg
       61 tgacctggac atccaccatc ggtgcgaatc tgctggtagt cgtggatgat gttgtcgcta
      121 acgatgatat tcaacagaaa ttaatgggta ttaccgcaga aacctacggc tttggcattc
      181 gtttctttac tatcgaaaaa accattaacg tcatcggcaa agctgcaccg catcagaaga
      241 tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc
      301 tgaaagatat caacgtcggc aatatgcatt tctcggaagg aaaaaagcaa atcagcagta
      361 aagtttatgt cgatgaccag gatctcgcgg acttacgttt tattaaacaa cgtggcgtga
      421 atgttttcat tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa
      481 atcgccttaa tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt
      541 atccctggcg gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt
      601 attccgcccg ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg
      661 cttaattacc ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt
      721 tcagccgccc aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg
      781 cgttgatgcc aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt
      841 cattctgttc ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa
      901 agaggcggat acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc
      961 agcgtatgcg gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct
     1021 ggtgaaagcg atgcccgcct ggttgaccca cggctttgaa gtggctggcg gtattctgcc
     1081 tgccgttggt tttggcttgc tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct
     1141 gatcgccggt ttcctgtttg tttgctacat ccaggtcagc aacctgttgc cggttgccgt
     1201 actgggcgca ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca
     1261 accgcagccc gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca
     1321 gtaaaaaaga tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact
     1381 acgaaaggat gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt
     1441 ataaggacga caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata
     1501 cccacccgaa tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag
     1561 aaaaccgcga caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg
     1621 gcgatgcgat tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg
     1681 ccagccaggg aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt
     1741 tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag
     1801 tgcgagagaa ctcgcagatg attgctcgct cggcaaccat cctcggaatc acggtaatcg
     1861 gcgggctgat cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta
     1921 cccacagcgt cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga
     1981 tggcctacac cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt
     2041 taatcggcgt gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg
     2101 aacgaggcac tgcttctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc
     2161 ttcagcaagt tgaaaactat acggcgttaa gtgaacgagc cagcgaatat ttattggctg
     2221 tgatccgtag caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga
     2281 cgtatcatta tctggtagaa aaaatccacc agcagcaggt tgatatcagc cagctcacct
     2341 tcgtgaagct cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt
     2401 tcctacaaca gcatatcgtg cagccgctgg ggctgcgtga agaccagctc atcagctttc
     2461 gctccgaaga gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag
     2521 gcggtctgga tttatgcgtt ctaggattgg ggaaaaacgg tcatcttggg ctgaacgaac
     2581 cgggagaaag cctacaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac
     2641 atgagatgtt aaaaaccacg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg
     2701 atattctcaa tgcccgcgag gttttgttat tggtgactgg cgaaggaaag caggatgcga
     2761 cagaacgttt tctcacggct aaagtctcca ccgctatccc ggcttcgttt ttatggctgc
     2821 acagtaactt tatttgttta attaatacct aaccagatga aaaatttgct caaaa
//
LOCUS       DAPPNJ010000316         2859 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000316 DAPPNJ010000000
VERSION     DAPPNJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.318"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(38..310)
                     /gene="rcnR"
                     /locus_tag="RPM73_004354"
     CDS             complement(38..310)
                     /gene="rcnR"
                     /locus_tag="RPM73_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDX6522613.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            431..1255
                     /gene="rcnA"
                     /locus_tag="RPM73_004355"
     CDS             431..1255
                     /gene="rcnA"
                     /locus_tag="RPM73_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDX6522614.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1474..1812
                     /gene="rcnB"
                     /locus_tag="RPM73_004356"
     CDS             1474..1812
                     /gene="rcnB"
                     /locus_tag="RPM73_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDX6522615.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1894..>2859)
                     /locus_tag="RPM73_004357"
     CDS             complement(1894..>2859)
                     /locus_tag="RPM73_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDX6522616.1"
                     /translation="SELSVQHNLVVQGDFALTQTQMATYEHNFNDSSCVSTNTITPMS
                     PSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAASNAKVNVSAG
                     GGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVYSF
                     NLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIILRCKNLLGQQN
                     QTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGSPVTLLNITNSSKGYTNLK
                     EIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT          739 a          653 c          632 g          835 t
ORIGIN      
        1 cgaggaaaaa cggcagtttt acaatcgcgt taataaatta tttgatatat gaatccagca
       61 ccttcagaac gacatccaga tcttcttcac gttttagctc atccccctgg tgaacgatgt
      121 gttccgtcag atgaccttta atcacttccc gcatcagacc gtttaccgcg ccacggatag
      181 cagcaatctg ttgtaaaact gcagcgcatt cgtgcggctc gtcgagcatt ttcttgagcg
      241 ccacgacctg accctgaatc ttactggcac gcgctttcag tttctgttta tcacggattg
      301 tatgagacat ggcaacacct tgttaacaag aacatgaaaa atcatagcac tattaatcta
      361 ctggggggta gtatcaggta ctggggggga gtagaatcag attgccgaat taatactaag
      421 aattattatc atgaccgaat ttacaactct tcttcagcaa ggaaacgcct ggttcttcat
      481 ccccagcgcc atcttacttg gtgcgcttca tggcctggaa ccagggcact caaaaacgat
      541 gatggcggcg tttatcatcg ccatcaaagg caccattaaa caggcggtga tgctcggact
      601 ggcagcaact atttcgcata ccgcagtggt ctggttgatt gcctttggcg ggatggtggt
      661 cagcaagcgc tttactgctc aatcagcaga accgtggctc cagttgattt ccgcagtgat
      721 cattattagc accgcgttct ggatgttctg gcgtacctgg cacggcgagc gcaactggct
      781 ggagaatatg cacgagcatg atcatgagca tcatcatcac gatcacgaag atcaccacga
      841 ccatggacat catcaccatc acgaacatgg cgagtatcag gatgctcatg cacgagccca
      901 tgctaatgac attaaacgac gctttgatgg tagagaggtc accaactggc aaattttgct
      961 atttggctta accggtggcc ttatcccctg cccggcagca attaccgtgc tgttgatttg
     1021 cattcagttg aaagccctga cactgggcgc cacactggtc gtcagtttca gcattggcct
     1081 ggcgttaacg cttgtcaccg taggcgttgg cgcagcaatc agcgttcagc aggtcgcaaa
     1141 acgctggagc ggatttaaca ctctcgctaa acgcgccccc tatttttcca gtctgttgat
     1201 tggcttagtt ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc gataactcaa
     1261 tcctttaggc ttgcatgtta tcttcagccc aggatgattc ttaaatcagc tatttccgct
     1321 gactctctgc tcgctaagga tgcttttagg gcatcctttc atttacactt tttacgaaat
     1381 catgggatca ctaacaaaat atcgcttgtc agttatattg tatggcagga aagatatgcg
     1441 actgatatta cagatcccca aagtggagag tttatgacca ttaaaaataa gatgttgctg
     1501 ggtgcgcttt tgctggttac cagtgccgcc tgggccgcac cagccaccgc gggttcgacc
     1561 aatacctcgg gaatttctaa gtatgagtta agcagtttca ttgctgactt taagcatttc
     1621 aaaccaggcg ataccgtccc agagatgtac cgcaccgatg agtacaacat taagcagtgg
     1681 cagttacgta acctgcccgc gcctgatgcc gggacgcact ggacctatat gggtggcgcg
     1741 tacgtgttga tcagcgacac cgacggtaaa atcattaaag cctacgacgg tgagattttt
     1801 tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa tgcagacacc ttgttatttt
     1861 ttattattag ccacttgctc gtcttgcttg ttattagtcg tatttcacgt tgattagtgc
     1921 ggttgcctcc agtgcgccag atttaacttt gtttgtatcg tagacgtagt aactggctgt
     1981 tatcggaatt gaaaccgttg tatctgtaag ttttgacttt gccgcaattt cttttaaatt
     2041 tgtgtatcct ttactgctgt tagtgatatt taaaagtgtg actggcgaac cattactttc
     2101 aagaataaat cctacgccat tatcttccgc acctttcagt attgtgttgc tgttggttaa
     2161 caagtcagaa cttgataaat acacctgcat tttccgtgat gtttgatttt gttgacctaa
     2221 taaattttta caacgcagaa taatatcccc ttgtttacta tttattgttg ctttgttacc
     2281 tgtggctggt aattgtgaaa cgggaatact gtctaaagta attaataaat cgtccggttt
     2341 gcaggttgtg gttatcgggt tgtatgacaa tgtcagatta aaggaataga cacctgcatt
     2401 ttgtgccaat gcatttctgt agctatttac acatgcatcc catgacttgc cacctaataa
     2461 acacagtaga aattgtactg cggcactccc gcgtgtcgat gatgaagcac tggataatgt
     2521 cgcaacacca ttaatcatca ctgaaccgcc gccccccgca gaaacattca cttttgcatt
     2581 actggccgca ggtgtcaccg taactgaata accactggtg aaactggtct gattatttga
     2641 caacgtgatg ttgtttttat tggtccattc aaaatgtaaa tttaatttaa tggtatcgtt
     2701 ataaagtcca acaataatat ccgacgggct cataggggtg atagtatttg tactcacgca
     2761 tgacgaatca ttaaaattat gctcataggt cgccatttgt gtttgagtaa gtgcaaaatc
     2821 cccctgaaca accaaattgt gctgaacaga taactcact
//
LOCUS       DAPPNJ010000317         2857 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000317 DAPPNJ010000000
VERSION     DAPPNJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.319"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            167..2446
                     /gene="ydeP"
                     /locus_tag="RPM73_004358"
     CDS             167..2446
                     /gene="ydeP"
                     /locus_tag="RPM73_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDX6522617.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICHVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2694..>2857
                     /gene="safA"
                     /locus_tag="RPM73_004359"
     CDS             2694..>2857
                     /gene="safA"
                     /locus_tag="RPM73_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDX6522618.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDL"
BASE COUNT          767 a          659 c          693 g          738 t
ORIGIN      
        1 gcttacctta tcgtatttaa tctattgttt aacgataata gattaaaaat agaaagagag
       61 tagtccccta ctcttatata tccatgttgg cgataatccc tttgtatgta ctgtgcatca
      121 tcgctattac aaatcctaat aattcatttc cacacaggat aagtagatga agaaaaaaat
      181 tgaatcctac cagggtgctg ccggtggttg gggtgctgtt aaatccgtag cgaatgcagt
      241 acgtaagcag atggatatac gccaggatgt tattgccatg tttgacatga ataagccaga
      301 gggctttgac tgtccgggtt gtgcatggcc agatcctaag cacagtgcgt cattcgacat
      361 ttgtgaaaac ggcgcaaaag caatcgcctg ggaagtcacg gataagcagg taaacgcctc
      421 tttctttgct gagaatacgg ttcaatcatt acttacctgg ggagaccacg agcttgaggc
      481 tgcggggcga ctcactcagc ctttgaaata tgatgccgtc agcgactgtt acaagccatt
      541 aagctggcaa caagctttcg atgaaattgg cgcacgcctt caaagctata gtgatcccaa
      601 tcaggttgaa ttctatactt cgggccgcac ttccaatgaa gctgcctttc tttatcagct
      661 ttttgcccgt gaatacggga gcaataactt tcccgactgc tccaacatgt gccatgaacc
      721 gacaagcgtg ggtttggcag cgagtatcgg tgtaggtaaa gggaccgtgt tgctggaaga
      781 ctttgagaag tgcgatttag tcatttgcat tgggcataac cctggtacaa accaccctcg
      841 catgctgact tcgttgcgcg ctttagtgaa acggggagcg aaaatgatcg ccatcaatcc
      901 tctacaggaa cgtggcctgg agcgatttac tgcaccgcaa aacccgtttg aaatgctgac
      961 gaactctgag actcagttgg ccagtgccta ctataacgtg cgcattggtg gtgatatggc
     1021 gttgctcaag gggatgatgc gcctgttaat tgagcgcgat gatgctgcaa gcgccgcagg
     1081 tcggccctca ttgctggatg acgaatttat tcaaacgcat accgtcggct ttgacgagct
     1141 acgccgtgac gttctcaatt ccgagtggaa agatatcgaa cgtatttctg gattaagtca
     1201 gactcaaatc gccgaactgg ctgatgcata tgccgctgcc gaacgaacca ttatctgtta
     1261 cggaatgggg atcactcagc acgaacatgg tacccagaat gtacagcaac tggtcaatct
     1321 gctgttgatg aaaggtaaca ttggcaagcc tggtgcgggt atctgcccac tacgtggcca
     1381 ctctaatgta cagggcgacc gaaccgtcgg tatcaccgag aaaccgtctg cagagtttct
     1441 ggctcgcctg ggtgagcgct atggcttcac accacctcaa gcacctggac atgctgcaat
     1501 tgccagcatg caagcaatat gtacagggca ggctcgagca ttgatctgca tggggggcaa
     1561 ctttgcactg gcaatgccag atcgggaagc gagcgctgta ccgttaacgc aattagatct
     1621 ggcggtacac gtagccacta agcttaaccg ctctcatctg ctgaccgcac ggcatagcta
     1681 tattctgccg gttctgggac gtagcgagat tgacatgcaa aaaagcggtg cgcaggcggt
     1741 aaccgttgag gattcaatgt cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg
     1801 tgtaatgctg aaatcagagt gtgcagtggt cgcgggaatc gcgcaggcag cactacccca
     1861 gagcgtggta gcctgggagt atctggtgga agattatgat cgcattcgca atgacattga
     1921 agctgtgctg ccagagttcg ccgattataa ccagcgcatc cgacatcctg gtggttttca
     1981 cctgataaac gctgctgctg aaaggcgctg gatgactcag tcaggtaagg ctaatttcat
     2041 taccagcaaa gggctgttag aagatccctc ttcagcgttt aacagtaagc tggtcatggc
     2101 gacagtacgc agccacgatc agtacaacac gacgatttat ggtatggatg atcgctatcg
     2161 aggggtattc ggtcaacgag atgtggtctt tatgagtgct aaacaagcta aaatttgcca
     2221 tgtaaaaaac ggcgaaagag ttaatcttat tgcgcttacg ccagacggta agcgcagctc
     2281 acgccgcatg gatagattaa aagtggtcat ttaccctatg gctgaccgct cactagtgac
     2341 ctattttcca gaatcgaatc acatgctaac acttgataac cacgatccat taagtggcat
     2401 tcctggctat aaaagtattc cggttgaatt agaaccatca aattaatgcc tcttctcatt
     2461 tcttctgctg tcatccgcac agcagaagaa tccctcattg actattatcg cgcaatttgc
     2521 tcacatggat taaattaaac tacatactat aagatataaa cttctgccta cagctgtaag
     2581 aaactccgtt cagtactgaa gcgccagtcc tatttcctct tttctccggc ctgttatatt
     2641 aagcatactg attaacgatg cttaacgtta ttcgcttaat aaacatattt gaaatgcatg
     2701 cgaccacagt gaaaaacaaa atcacgcaaa gagacaacta taaagaaatc atgtctgtaa
     2761 ttgtggttgt cttattactg acccttacgt tgatagccat tttttcggca attgatcagc
     2821 tgggcatttc agaaatgggt cgcattgcaa gagatct
//
LOCUS       DAPPNJ010000318         2816 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000318 DAPPNJ010000000
VERSION     DAPPNJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.320"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            78..836
                     /locus_tag="RPM73_004360"
     CDS             78..836
                     /locus_tag="RPM73_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDX6522619.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(889..996)
                     /locus_tag="RPM73_004361"
     CDS             complement(889..996)
                     /locus_tag="RPM73_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522620.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1172..2816)
                     /locus_tag="RPM73_004362"
                     /pseudo
     CDS             complement(1172..2816)
                     /locus_tag="RPM73_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          780 a          556 c          626 g          854 t
ORIGIN      
        1 ggacgctaat ctttgatcct taagaaagtt tccgggaccg cgatggtctc tttttttgtt
       61 gtacggactc ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg
      121 ggggcatgtt tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg
      181 atcctgccag cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat
      241 caggcaatac gaaaagctcc tcacttacgg ctgacaccac catgacctgg tatggacaaa
      301 ccactgcctg gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt
      361 cagaaaaata tgcggcgggc gggcgtagcc gttttaattt aactgattat gactatttat
      421 ttggtcaggc aagctggctg acagaccgtt ataacggcta tcgagagcgc gatgtgttga
      481 ccgcgggtta tggtcgtcag tttttgaatg gcccggtaca cagtttccgc tttgaattcg
      541 gtccaggtgt gcgttacgac aaatataccg acaatgccag tgaaactcag ccgctgggct
      601 atgcctccgg tgcttatgca tggcagttga ctgacaacgc taaatttacg cagggtgttt
      661 ccgtctttgg cgcggaagac acaacgctga actccgaaag cgcattaaat gttgctatta
      721 atgaacattt tggactgaaa gtggcttaca acgtcacatg gaactctgaa cctccagagt
      781 ctgcaccgga acataccgat cgtcggacaa ccttatcgct gggctacagc atgtaaaatt
      841 atctgccgga ccgtgttatg cggtctggct gtcttcgttt gtcactgctc agccccaaat
      901 cattgctgcc caacgtagca acaacatagc gccacagatt gccagtagca ccaccaccat
      961 cttccagtgt tttttatcaa agcgtttagt cggcatacca gtgtcctgca tatctcttgt
     1021 gcgtgataac ggagtgtaac aaaagggaca ggcatcaagg gattttttgt tattacagcc
     1081 gtcgggtggc gttgtacacc tgatcactgt ttctgcgttt tatgaaaaaa ataaaattat
     1141 gtatcgatat acaaatatca taaatagctg gttataaata cgtttttagt tcatcagcac
     1201 taaaactcag gctttggctg attgcattaa tagcagcata atatgtttct acagttttat
     1261 aatgattatg atgaatggct atatgaacag ctgacatgtt ttcatgattt ttagcggcca
     1321 acagtgtgaa taattgacgt tgactaaaag aatatttttt tgcaaaagta ctcagtgttg
     1381 atatataaga taacacgacg tctttattac ccttgctcat ggcgatgtat aaagcagggg
     1441 ttccatgtgc tgtcgcgcct tttaataagt ccatgatgtt gattttgctg agtttatatt
     1501 taacggcgat cccataaact ttagagagga accgtgtgaa acacaaaggg tgattatttt
     1561 ccattgcagc aaataaccct agttcaccat cacgtgtttt catggctaaa atatccatca
     1621 ccattttttt gtttaaaagt tcttgtgcta ttggagtggt taatgcattc agaaagatat
     1681 cgattatttc atcgaatcca tacaacatgg agatatagag tccaggtgtt tcgttagcac
     1741 ttttagtttg aagtaattta accagattat cttcatgaat gatatgattg tataccagtg
     1801 actggatttc ttgcataaat atttttacgt tgtcagtttg tccgttcatc atagccagaa
     1861 ataaagcagg tgtaccaacg ctattctgcg cagttattat ttcaatgatt tctttttcag
     1921 ggagtatggg caactgtttg aatagtgtaa taagctattg agtctggcct gtggccatta
     1981 ttaacaacat tgccgatgga tgtatagggg caaatggcat tcgtgtcgtg atacccgcta
     2041 aaatagcaac tggaactccc tcaggtaata gaggaagaat agcaattgca cagttgcgaa
     2101 catacctatc agcccaggtc tgagcatcgg gagctgaact gatgaactta tcaagcgaga
     2161 gaaagcgccg tgctgtcctg tgggtgcctc tgacggcaac gttggtatcg ttcgggtcgt
     2221 aaaaagacac aacataattt aattgtccat acactgactg ctttattcgt agccttactg
     2281 acaaagtatg attgtctata tataaaatcg cggacatatg actgacattg tttgccatca
     2341 ttcgacaaaa gaaaatatct aaagcgaagc caaaatcctt actcgcgata acgcgactcc
     2401 ctgcaggagg atggcgaagt aatgctctga actccgcatc ccggactaag gggatatgct
     2461 gctgtatgga ttcaacggag gagtaatgtt taatcaggtt gactttgcct tttccatttc
     2521 tcaatgaatc aagggcgtat tgcaatgaca gatggcgaca aaaaatagcg tcagaaggag
     2581 attgcaaaaa acatgcacta ccattgagat ttactgttgg gtgtatagtt ttttctctat
     2641 ggattttttt agacagtttc atacaatggt tacgatcttc agcagttaac tgttcctgtt
     2701 caatataatc cagtatgctt cgcaaatggt tagctttaat aggcgtggtt tccagtaata
     2761 aggaaatgaa atctttttta tagtgtaatg taatgagtat attttctgca tggatg
//
LOCUS       DAPPNJ010000319         2711 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000319 DAPPNJ010000000
VERSION     DAPPNJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.321"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            192..1358
                     /gene="nhaA"
                     /locus_tag="RPM73_004363"
     CDS             192..1358
                     /gene="nhaA"
                     /locus_tag="RPM73_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDX6522621.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1424..2323
                     /gene="nhaR"
                     /locus_tag="RPM73_004364"
     CDS             1424..2323
                     /gene="nhaR"
                     /locus_tag="RPM73_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDX6522622.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2362..>2711)
                     /locus_tag="RPM73_004365"
     CDS             complement(2362..>2711)
                     /locus_tag="RPM73_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDX6522623.1"
                     /translation="FHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNEL
                     ENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSG
                     QIMSRVKIVVMYN"
BASE COUNT          630 a          604 c          691 g          786 t
ORIGIN      
        1 tatgattatc agggcttagg gtcacccgtt agcgcttaca gccgtcaaaa actcatctca
       61 ccgctgatgg cgcaaatcct ttaattgctc gtaaaaaacg agttattcct acactataat
      121 ctgattttaa cgatgatttg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      181 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      241 ttcttatcat tgccgctatc ctggcgatga ttatggccaa tagcggcgca accagtggat
      301 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      361 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      421 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
      481 cagttatcgc cgctattggt gggatgattg ttccggcatt actctatctg gcctttaact
      541 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcctttg
      601 cacttggtgt gctggcgctg ttgggaagtc gtgttccgtt agcgctgaag atctttttga
      661 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
      721 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
      781 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct ggttggcgta gtgttgtgga
      841 ctgcggtgtt gaaatcgggg gttcacgcaa ccctggcggg ggtaattgtc ggcttcttta
      901 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttacacc
      961 cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc
     1021 aaggtgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1081 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1141 cgcatctgcc tgagggaacg acttatcagc aaattatggc ggtggggatc ctgtgcggta
     1201 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1261 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1321 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1381 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1441 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     1501 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     1561 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     1621 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     1681 ctatcgcaaa gaatccaatt tattgtttga cgttggtgtg gctgatgcac tttccaaacg
     1741 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     1801 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     1861 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     1921 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     1981 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2041 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2101 gttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2161 atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2221 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2281 caatacggat tattctgcgc tttttagtcc agcggcgcgt taatcggcac ctccccccaa
     2341 agttaaggtg ggggagatag attagttgta cataaccacg attttgactc ggctcattat
     2401 ttgcccgctt gagacattat ttccatatgt acgcgggcga ataaatagag gaatctgatt
     2461 acttccttca tggggatgct gaaaagagta gtaattgctt ttaccatact ggatttcatt
     2521 cgtaccatca ttggagagtt gaaagcctat ttgacccgca ccgtcctcac tgttttctaa
     2581 ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc actggttcaa agtaatattc
     2641 aacatcactg ggttcaaaaa ctaattcacc ttgctcgccc tggatgcagt caaatcttat
     2701 atgatgaaac t
//
LOCUS       DAPPNJ010000320         2681 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000320 DAPPNJ010000000
VERSION     DAPPNJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.322"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1140
                     /gene="cpsG"
                     /locus_tag="RPM73_004366"
                     /gene_synonym="manB"
     CDS             <1..1140
                     /gene="cpsG"
                     /locus_tag="RPM73_004366"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDX6522624.1"
                     /translation="EEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDT
                     GLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGN
                     GAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHG
                     ADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVD
                     VVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAE
                     LVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGIS
                     MTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLILELLSK"
     gene            1304..>2681
                     /gene="gndA"
                     /locus_tag="RPM73_004367"
     CDS             1304..>2681
                     /gene="gndA"
                     /locus_tag="RPM73_004367"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDX6522625.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENAQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKE"
BASE COUNT          669 a          683 c          712 g          617 t
ORIGIN      
        1 gaagagatct atttcgccac gttccatctc ggcgtggatg gcggcattga agttaccgcc
       61 agccataatc cgatggatta caatggcatg aagctggtgc gcgaaggggc tcgcccgatc
      121 agcggtgata ccggactgcg cgacgtccag cgcctggcag aagccaacga ctttcctccc
      181 gtcgatgaaa ccaaacgcgg tcgctatcag caaatcaatc tgcgtgacgc ttacgttgat
      241 cacctgttcg gttatatcaa tgtcaaaaat ctcacgccgc tcaagctggt gattaactcc
      301 gggaacggcg cagcgggtcc ggtggtggac gccatcgaag cccgctttaa agccctcggc
      361 gcgcccgtgg aattaatcaa agtgcacaac acgccggacg gtaatttccc caacggtatt
      421 cctaacccgc tactgccaga atgccgcgac gacacccgca atgcggtcat taaacacggc
      481 gcggatatgg gcattgcttt tgatggcgat tttgaccgct gcttcctgtt tgacgaaaaa
      541 gggcagttta ttgagggcta ctacattgtc ggcctgctgg cagaagcgtt cctcgaaaaa
      601 aatcctggcg cgaagatcat ccacgatcca cgtctctcct ggaacaccgt tgatgtggtg
      661 actgccgcag gtggtacgcc ggtaatgtcg aaaaccggac acgcctttat taaagaacgt
      721 atgcgcaagg aagacgccat ctacggtggc gaaatgagcg cccaccacta tttccgtgat
      781 ttcgcctact gcgacagcgg catgatcccg tggctgctgg tggctgaact ggtgtgtctg
      841 aaaggaaaaa cgctgggcga actggtgcgc gaccggatgg cagcatttcc ggcaagcggt
      901 gagatcaaca gcaaactggc gcaacccgtt gaggcgatta accgcgtcga acagcatttt
      961 agccgtgagg cgctggcggt ggatcgcacc gatggcatca gcatgacctt tgccgactgg
     1021 cgctttaacc tgcgctcctc caacaccgaa ccggtggtgc ggttgaatgt ggaatcgcgc
     1081 ggtgatgtgc cgctgatgga agaaaaaaca aaacttatcc ttgagttact gagtaagtaa
     1141 tccagtaatt tcatataaat aggttttaaa agacggaaaa gatgagatat acagtgtggt
     1201 atagccaagg taatgctatt caccatctct attagtgagt taacatctat accacattta
     1261 agccgcacac tcggcggtga gcaccccctg acaggagtaa acaatgtcaa agcaacagat
     1321 cggcgtcgtc ggtatggcag tgatggggcg caaccttgcg ctcaacatcg aaagccgtgg
     1381 ttataccgtc tctattttca accgttcccg tgaaaagacg gaagaagtta ttgccgaaaa
     1441 tccaggcaag aaactggttc cttactatac ggtgaaagag tttgttgaat ctctggaaac
     1501 gcctcgtcgc atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc
     1561 cctcaagcca tacctcgata aaggtgacat catcattgat ggtggtaaca ccttcttcca
     1621 ggacaccatt cgtcgtaacc gtgagctttc tgcagaaggc tttaacttta tcggtactgg
     1681 tgtttccggt ggtgaagaag gtgcgctgaa aggtccttcc attatgcctg gcggacagaa
     1741 agaagcctat gaactggttg caccgatcct gaccaaaatc gccgcagtgg ctgaagacgg
     1801 ggagccatgc gttacctata ttggtgccga tggcgcaggt cactatgtta agatggttca
     1861 caacggtatt gaatacggtg atatgcagct gattgctgaa gcctattctc tgcttaaagg
     1921 cggcctgaat ctctctaacg aagaactggc acagaccttt accgagtgga ataacggtga
     1981 actgagcagc tacctgatcg acatcaccaa agacatcttc actaaaaaag atgaagacgg
     2041 taactacctg gttgatgtga tcctggatga agcggctaac aaaggtaccg gtaaatggac
     2101 cagccagagc gcgctggatc tcggagaacc gctgtcgctg attaccgagt ctgtgtttgc
     2161 acgttatatc tcttctctga aagatcagcg cgttgccgca tctaaagttc tctctggtcc
     2221 gcaagcgcag ccagctggcg acaaggctga gttcatcgaa aaagttcgtc gtgcgctgta
     2281 tctgggcaaa atcgtttctt acgctcaggg cttctctcag ctgcgtgctg cgtctgaaga
     2341 gtacaactgg gatctgaact acggcgaaat cgcgaagatt ttccgtgctg gctgcatcat
     2401 ccgtgcgcag ttcctgcaga aaatcaccga tgcatatgcc gaaaatgcgc agatcgctaa
     2461 cctgctgctg gccccgtact tcaagcaaat tgccgatgac taccagcagg cgctgcgcga
     2521 tgtcgtcgct tatgcagtac agaacggtat cccggttccg accttcgccg ctgcggttgc
     2581 ctattacgat agctaccgtg ccgctgttct gcctgcgaac ctgatccagg cacagcgtga
     2641 ctatttcggg gcgcatactt ataagcgcat tgataaagaa g
//
LOCUS       DAPPNJ010000321         2661 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000321 DAPPNJ010000000
VERSION     DAPPNJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.323"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(57..1247)
                     /gene="aspC"
                     /locus_tag="RPM73_004368"
     CDS             complement(57..1247)
                     /gene="aspC"
                     /locus_tag="RPM73_004368"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDX6522626.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1432..2520)
                     /gene="ompF"
                     /locus_tag="RPM73_004369"
     CDS             complement(1432..2520)
                     /gene="ompF"
                     /locus_tag="RPM73_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDX6522627.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          659 a          658 c          638 g          706 t
ORIGIN      
        1 ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt taatgcttac
       61 agcactgcca caatcgcttc gcacagcgga gccatgttat ctggtgtcat cccggccaca
      121 tttacgcgac cagaagcaac cgcatatacg ccaaactctt cgcgcagacg cagcacttgt
      181 tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
      241 cggtttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
      301 tggcgcatat cagtcagctc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg
      361 gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct
      421 ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg cagcaaccag agtacaagcg
      481 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct
      541 ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaaa
      601 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caactgagag ttgtgccagt
      661 gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac
      721 agcactacgt cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg
      781 gtgtggtttt ctgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
      841 acgctcttat ggttcggcca gcttgggttg ctcacccaca ctcgcttaac gctggtattt
      901 tttgccagga aatcggcagc tacgcgtagt gcgccagtgc cccccggagt ctgtgccgtg
      961 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
     1021 cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
     1081 agatactgtt cagccttttt cacgctggtc aataccgggg ttttgcccgt ctcatcttta
     1141 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
     1201 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
     1261 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
     1321 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa
     1381 aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
     1441 gtaaacgata cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc
     1501 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc
     1561 gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt ctttcgcttt
     1621 agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa
     1681 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat tagtgatcgg
     1741 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcgtactt
     1801 cagaccagta gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag
     1861 gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt attcgtagct
     1921 gatagaaccg ccaacaccgt cgccgttaga acggcgtgca gtgtcacgct cgtttttacc
     1981 caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata
     2041 ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag tatcaccacc
     2101 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
     2161 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
     2221 accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc
     2281 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
     2341 catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggaaaaat aatgcagacc
     2401 aacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
     2461 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
     2521 tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat aagttctgtc
     2581 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct
     2641 ttccattcaa actaacgttt c
//
LOCUS       DAPPNJ010000322         2654 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000322 DAPPNJ010000000
VERSION     DAPPNJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.324"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(1..432)
                     /locus_tag="RPM73_004370"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="RPM73_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            436..606
                     /locus_tag="RPM73_004371"
                     /pseudo
     CDS             436..606
                     /locus_tag="RPM73_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(683..1511)
                     /locus_tag="RPM73_004372"
                     /pseudo
     CDS             complement(683..1511)
                     /locus_tag="RPM73_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(1717..1947)
                     /locus_tag="RPM73_004373"
     CDS             complement(1717..1947)
                     /locus_tag="RPM73_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDX6522628.1"
                     /translation="MQRHGSIETRTARDKAAERQMSHMDVTQRRPRIIHKAEYLDERK
                     LMLQWWVDFLDANRERAITPFDYAKINRGNGE"
     gene            2179..2547
                     /locus_tag="RPM73_004374"
     CDS             2179..2547
                     /locus_tag="RPM73_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522629.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEIEKRKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     IGKNYIKRRLSVKYKKAEVL"
BASE COUNT          699 a          641 c          579 g          735 t
ORIGIN      
        1 atttccagac atctgttatc acttaaccca ttactgttcc accgccgcag acactcctgt
       61 ggcgagtggt aacaccaaag cactatgcag ataccattag ttatagtgtc cgaatgcctc
      121 cgcaagtttt ttgctgccgt taacccgtcc ggtttgggca tgatactgat gtaatcattt
      181 cacgaagctc tctgctattc cgttactctc cggactccgc accgccgtgt tcttcggttc
      241 aagtcccaac atccgggtga actggcgtgt ttcattagcc cggtagcatg aaccattatc
      301 cgttagccac tccactggat acgccggaag ctcgctgcca aagcggcgtt ccacggctct
      361 tggcatgacg tcctgtattg tttcactgtc gaagcagccc gtagtgaccg cccagtgtag
      421 tgcctcacgc tcgctgaagc gttttttctt ggcggtgtag agacaacaaa agtgctgaag
      481 gcgtttaggc cacacatttt ctttgatgat aaggatgttg atttgatcga ggctgcaaag
      541 gtagtacctt ctggtaaagt accttatgcc agtaactccg aactggcaga acgtgaagca
      601 tcgtgattat cactaacaaa ccatttagtc attaaggctg gatggtttgt ataccacacg
      661 caaaaaactg ccctacccca gatcaatgtt ctcctctgaa cacctctttt ctctgccatt
      721 ccacaaactg ttcgcccgga taattttctt taaccggtgg caacgcaatc gcttttcccg
      781 caaaatccca gaacagtcgc tgtaccactc caccaccatt taccgcaccg gaaaccacca
      841 cacgcatatt ttcatccagg ccaatcgaac cgcgatcaaa cactttatga tggattgcgc
      901 acaatgccag gccattaggt atttcacaag ggccatggtg ctgcttccat ctgatatgag
      961 caacttccag agcaatcggc gcattgtcgt ggcgcatgtt aaagccgcag atagcgcact
     1021 gataattgta agcacgtaga accatcttgc gaaaccccgg atcgcgttga cggagagaag
     1081 tgcgaatatc aaaatccatc tcgtcagcga tctcttcctg aatactggcc gggaaatttt
     1141 gtcaccaacg caaaattatc ggcatcaaat ccaccagcga cattatattc aatgagttca
     1201 cgtcggggcg gctggcggct accactggtt gagcacagtt ccgcgttatg tggttcccag
     1261 aagccatcgc ccttcagacg ccagaacggc atgtccgggc gctggtcacg gcgctgcggt
     1321 ccataacgtt ccaggagatc caacaggggc tcgtagattt cagaagcata gttaaacagg
     1381 cgaccatgac cctgccgata gtgtgagaga acgtacagca gcaataatgg cttatgtggc
     1441 gcacgttgtt cgcctttgcg ccagatggtg atatttgata tcgcttgctt tagggttttg
     1501 tcagaggtca ttgcgttgga gaatccatga gcacatgttt gcgatgatgc tcgcaactat
     1561 atgtacattc aaggcattat tgcagacatc ctttttgcct gacggctctg tacctggcag
     1621 atggaattct gaaaaaaata cagcgaccaa ccagtcctaa gcggtctggt tgatctttta
     1681 aatttaaaca tcagctaagg agtaacggca ttcaactcac tcgccattac cccgattgat
     1741 cttcgcatag tcaaacggcg taatcgctct ttcccgattg gcatccagaa aatcgaccca
     1801 ccactgcaac atcagcttac gctcatccag atattccgct ttatggatta tacgcggcct
     1861 gcgctgagtt acgtccatgt ggctcatctg acgttcagcc gctttatctc gtgctgttct
     1921 ggtctcaata cttccatgcc gttgcaatta cctcttaaac cattccttac cattttcatg
     1981 tcttcgataa gaaaatttca tcatgcatga tcgattttgt cgatcgattc gatcgaattt
     2041 tctcttttta ccctgccttt tgcatggttt taaaataggc aaaattactt gtcattcgtg
     2101 gaaaatgatg gcggagataa ctgagcttaa aaagtttctc ttgtctccgt cacttgccat
     2161 accagccagg aaaagaagat gtcagcacaa gaaaaaacag tacagattga ttcttttgat
     2221 gcgaaatttt tagatataaa tacattaagc aagcttatct atgaacgtaa gagttttgtg
     2281 atagaaaatg tttccgatgt tagtgaaacg gtaaaaagag tggagcatga aatagagaaa
     2341 aggaaattaa gttgtcgtgt atatactgag tacaggagca cagctttagc tggttcttta
     2401 tggtcaccaa ccgtaattct gggagtggcg agtgcagtag caataggtgt tcataattta
     2461 tctacatgga accccgatta tgaaatcggt aagaactata ttaaacggag attatcagta
     2521 aaatataaaa aggcagaagt attgtgatcg gttactccag tgcccctgcc tgttcgtatt
     2581 ctggtgaggt tgtatacaca taatttcaga ctggcgttca cgtcctcact gaggaaatat
     2641 atttattttg tgta
//
LOCUS       DAPPNJ010000323         2630 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000323 DAPPNJ010000000
VERSION     DAPPNJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.325"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            7..504
                     /locus_tag="RPM73_004375"
     CDS             7..504
                     /locus_tag="RPM73_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX6522630.1"
                     /translation="MSQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATELSAIHGHS
                     TSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTR
                     KAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLA
                     GLPAH"
     gene            complement(514..1941)
                     /locus_tag="RPM73_004376"
     CDS             complement(514..1941)
                     /locus_tag="RPM73_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDX6522631.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1938..>2630)
                     /locus_tag="RPM73_004377"
     CDS             complement(1938..>2630)
                     /locus_tag="RPM73_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDX6522632.1"
                     /translation="QKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDA
                     RDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAE
                     CCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGY
                     TFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYYS
                     ACPETLKDMSDL"
BASE COUNT          748 a          635 c          591 g          656 t
ORIGIN      
        1 acctgtatta gccaacccaa gaaaacaacg ttgcgtgata aagccatggt gaattatgcc
       61 tttgattatt taagctcacc aggcagtcta ccgttaacca cggcagccac ggagctttcc
      121 gcgattcatg gtcactcaac atcgcaatat cgccttggcg aattttatct tcatggtagc
      181 gacggtaaac cactggatta tacacaggcg agatactggt atgagcaatc agcggaacag
      241 gaaaatccac gcgcgcaaag taaactgggg tggatctacc tcaaaggtct gggggtcaaa
      301 cccgacaccc gtaaagcaat tctctggtat aaggaagcag ctgaacaagg gtatgctcat
      361 gctcaatata ctttaggttt gatctacaga aatggctcag gtattaatgt taaccattat
      421 gaatctcaaa aatggttaaa actggccgcc aaacaacatt acaaaaatgc ggaaagatta
      481 cttgccgggc ttcccgcaca ttaacgtcga ctgtcaaccg aacagatgga gtattttccc
      541 tatcaaacca gcaaagataa aaatatagaa aataatttta atgatgccca gacagcctgg
      601 cccaccatcg tcaacgggca ttggctgcgg ttgcggctcc ggttcaacag tttgcttctc
      661 ggtaaaattc gccgcctgaa ttaataatcg acgaataatt aatgcttcca gtggacaacg
      721 atcttctgca tcaaggcggc tcacaatcgg cattaatgat tcttcggttg gttggttcgc
      781 ctgcactaat tgtttggcaa ctaaatataa tggccataag ggatgttctg tatcacgcgt
      841 gagtgagatc cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg aacgccccag
      901 agcagagagt ttttcatcgc tgagtatttc gtataaatga aaacgcgtac gggcgctgtc
      961 gccggggata taaagtagcg gttttccctg atcgttaacc agttcacgag cttcaatcat
     1021 catcaccact aacgggtaat aatcgacccg atgctgacgg caccattgca atattaaagc
     1081 atccgcctgg gtggagggat actcgcgcca gtagtcacaa agttctgcca gcaagctttc
     1141 gccttctcca caatagacgc gttgtgcgta ttcataataa cgcgcatctt cattatcaga
     1201 ttcagcttcc cgccacgacc gggcgtaatc caggagttcg ggaattccca ggcgacctgc
     1261 gctataccag cggagtaaat ttctgtgcag ttttgcatca tcggggataa tcactgcgcg
     1321 atgttgcgct aaatacaatg tcagccattc tggatgcgat gaccaaatcc gagcgaacat
     1381 ttggtaaaaa tcgatggtgt cattttgcac ggcaacgggc agatgcagaa tggtctggaa
     1441 gttaaaaaca ttccccttcg caatggcata taaaaaggat tccagttctt cttcgtctat
     1501 ttccccgacg ttttcctcct gcaaccaccg caatctttct gctaataaca ccacgcactc
     1561 gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta attcatcaat
     1621 ctcttccatc gagcaataat ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa
     1681 acgttcgcta tcagaggcaa gtaacgcacg aaaggcgagc agaatttcat gttctgctac
     1741 ctcatattct tctggttgcc aaacagattt agcaggcgac tgcgcaatcc gtagcgcctc
     1801 ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat gaaaggacgg
     1861 taacagcgcc agataagcgc ggcggataat atcgacgtca gtcgtttcct ctatttcgag
     1921 aatcttccag caatttttca taggtcgctc atatccttaa gcgtttccgg gcatgcagaa
     1981 tagtaagcca atggttgcca ggacaaggta gggtaatacg catcatcact ggcaaataat
     2041 tgataaaaca tatcattgcc ggaatcgtcg tattctttac gcgctaagaa ttcgcgtaat
     2101 aactgcagat gatcatcttc gggagactgc caatattgcc gaccaaaggt ataaccggca
     2161 aagtattgcc gccagttacg ataataacgt agagcccgta gatgaatgcg cgactggatc
     2221 catagacttt cttcttcgct caaccagtta ttaagtacgc ccattcggct taaaaaaccc
     2281 atccggacat aatcccaggc tttaattcct ccctcaccac agcattcggc ggtgctggcg
     2341 acaaattgcg cataagcctg accttgctca tttagttcag caagatagtc acgccattca
     2401 catggcgaat aacgaaacca gcggtgataa aaccctgcca accgagcggc atgaccgtta
     2461 tcgatcattc ggtgaattgt gcgcagcagg tcatcacggg catcaattcc ccaggaggaa
     2521 gaaaggtcaa aaaagcatgt acctaaatac attttgggtg atgaatgcga aggtaaacag
     2581 cattcattgc gtatttccat cggagtggaa agtgcaaaca atattttttg
//
LOCUS       DAPPNJ010000324         2624 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000324 DAPPNJ010000000
VERSION     DAPPNJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.326"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(42..668)
                     /gene="upp"
                     /locus_tag="RPM73_004378"
     CDS             complement(42..668)
                     /gene="upp"
                     /locus_tag="RPM73_004378"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDX6522633.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            993..2030
                     /gene="purM"
                     /locus_tag="RPM73_004379"
     CDS             993..2030
                     /gene="purM"
                     /locus_tag="RPM73_004379"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDX6522634.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2030..>2624
                     /gene="purN"
                     /locus_tag="RPM73_004380"
     CDS             2030..>2624
                     /gene="purN"
                     /locus_tag="RPM73_004380"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDX6522635.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWL"
BASE COUNT          613 a          651 c          691 g          669 t
ORIGIN      
        1 aaaagccgac tcttaaagtc ggctttaatt atttttattc tttatttcgt accaaagatt
       61 ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag tccctgatca
      121 atcgatgcgg tatacagttc gacgtccggg tgcgcttttt ccagcgcagc gataccttct
      181 ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt cagcaggtcg
      241 atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag cgccatacgc
      301 tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt ttcttcatta
      361 cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac accgtccatc
      421 ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt gatctggtcg
      481 atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag gtcggcggtc
      541 gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg
      601 atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt gacttccacg
      661 atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgacgg attatacctc
      721 ctttcttcaa ggcggcaata ttcttttcgt tgactttagt caaaatgata acggtttgag
      781 ataaagttat tttatattca aatggttatg aaagaagatt attccatccg aaaactaacc
      841 tttaccctgg cacaagtctt ctttcgccgc gtgcctgggc aaaagacgtg caaaaaggtt
      901 gtgtaaagca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga attttatttt
      961 tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct cttagctaca
     1021 aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc aaaggcgtag
     1081 tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg
     1141 cattgccgca aaaatatcgt gaacctgtgc tggtttccgg tactgacggc gtaggtacca
     1201 agctgcgtct ggcgatggac ttaaaacgtc acgacaccat tggtattgat ctggtcgcca
     1261 tgtgcgttaa tgacctggtg gtgcaaggtg cagagccgct gtttttcctc gactattacg
     1321 caaccggaaa actggatgtt gataccgctt cagcggtgat cagcggtatc gcggaaggat
     1381 gtctgcaatc gggctgttca ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc
     1441 acggtgagga ttacgatgtc gcgggtttct gcgttggcgt ggtagaaaaa tcagaaatca
     1501 tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc agcggtccgc
     1561 actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa
     1621 ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc cgcatttacg
     1681 tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg cacctgaccg
     1741 gcggcggctt ctgggaaaac attccacgcg tattgccaga taatactcag gcagtgattg
     1801 atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca ggtaacgttg
     1861 agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc gccctgcctg
     1921 ctccggaagt ggacaaagcc ctcgccctgc taaactccaa cggtgaaaac gcgtggaaaa
     1981 tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa tgaatattgt
     2041 ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct gtaaaactaa
     2101 caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt tcggccttga
     2161 acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg
     2221 tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg atgtggtcgt
     2281 gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt
     2341 gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc atcgtcaggc
     2401 gctggaaaat ggtgatgaag agcacggtac atcggtgcat ttcgtcaccg atgaactgga
     2461 cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata cggaagatga
     2521 catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga ttagctggtt
     2581 tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctgg
//
LOCUS       DAPPNJ010000325         2502 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000325 DAPPNJ010000000
VERSION     DAPPNJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.327"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..100)
                     /locus_tag="RPM73_004381"
     CDS             complement(<1..100)
                     /locus_tag="RPM73_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522636.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKV"
     gene            154..2139
                     /gene="pdeR"
                     /locus_tag="RPM73_004382"
     CDS             154..2139
                     /gene="pdeR"
                     /locus_tag="RPM73_004382"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDX6522637.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            2286..2459
                     /locus_tag="RPM73_004383"
     CDS             2286..2459
                     /locus_tag="RPM73_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522638.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT          646 a          570 c          604 g          682 t
ORIGIN      
        1 tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa
       61 aaacaatgcg tttagccgcc gttaatttca tgtaagtcat gttgttcctt ttatttaacc
      121 gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga gtccacaacg
      181 ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag cagcgatgtt
      241 ttgcgctttt ctaccaccga aaccacagaa cctgatcgta cattgcagtt atctgccgaa
      301 caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat gagtctgacc
      361 gtcgatgaaa gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa acgggaatgg
      421 gctggcaacg cgtctgcatg gcatgacaca ccggcggttg ctcgtgattt atcacacggg
      481 ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat tctcgacagc
      541 cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt gaaagaacac
      601 gacgtcattg ggcaaagcgt gtttaaactg tttatgagcc gtcgtgaagc tgcggcatcc
      661 aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga actgtggata
      721 ccaacatgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca cagcggcagt
      781 ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga gcgccgcgct
      841 caggagcgac tgcgtattct ggcaaatacc gacagtatca ctggactgcc gaatcgtaac
      901 gcaatgcagg atttaatcga tcacgctatt aatcatgcag ataacaataa agttggggtt
      961 gtgtatcttg atttggataa tttcaaaaag gtcaacgacg cctatgggca tttgtttggt
     1021 gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca tgaccaggtg
     1081 ttggcgcgtc caggtgggga tgagtttctg gtactggcat ccaacacctc acaaagcgcg
     1141 ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg cattggttta
     1201 attgaagttt ataccagctg ttcagtagga attgcactct ctcccgaaca tggttcagac
     1261 agcacggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga aggcggacga
     1321 ggacaatttt gcgtttttac cccagaaatg aatcaacggg tatttgaata tctctggctg
     1381 gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta tcaaccgaaa
     1441 atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca gtcacctgaa
     1501 cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg gctaattgtg
     1561 cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg gcgggataaa
     1621 ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga tcaaaccatt
     1681 ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc tatagatgtt
     1741 gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt tattcaacaa
     1801 tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta ctcttcactt
     1861 tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt tgttcgagat
     1921 attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt ggcccaggca
     1981 ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc ttttttaacc
     2041 aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc cgccgtcgcc
     2101 ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata tatatactta
     2161 gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt tgctgttaaa
     2221 cacttctctt ttagcggcaa tttttgcatt attcattttc cacttttact ataaggatgt
     2281 ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg tattgatatt gtgttagata
     2341 tccttgtggc aggcgattac cactctgcga tccataatct cgagatactt aaagcagaac
     2401 tcttgcgcca ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg gagatttaac
     2461 aatcgtgcct tcaggttttc ctatttgagg atgcccggcg cg
//
LOCUS       DAPPNJ010000326         2497 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000326 DAPPNJ010000000
VERSION     DAPPNJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.328"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..106)
                     /locus_tag="RPM73_004384"
     CDS             complement(<1..106)
                     /locus_tag="RPM73_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6522639.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIY"
     gene            929..1156
                     /locus_tag="RPM73_004385"
     CDS             929..1156
                     /locus_tag="RPM73_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522640.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(1191..1331)
                     /gene="ykgO"
                     /locus_tag="RPM73_004386"
     CDS             complement(1191..1331)
                     /gene="ykgO"
                     /locus_tag="RPM73_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDX6522641.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1331..1594)
                     /locus_tag="RPM73_004387"
     CDS             complement(1331..1594)
                     /locus_tag="RPM73_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDX6522642.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            complement(1957..2058)
                     /locus_tag="RPM73_004388"
     CDS             complement(1957..2058)
                     /locus_tag="RPM73_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522643.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT          739 a          458 c          539 g          761 t
ORIGIN      
        1 gataaattaa tctgtcgata tccacaatca gttcagatag tcctttatag aagtaggcgt
       61 catggggaga ccagatgtat ttatcagaac ggttttgaca ttccatatgg tttttaacct
      121 catagtccct gctgtaatca ctttgccatg tcactacttt ccaaacctgt aatttttaca
      181 ggtgcattca gtttccttag taaaccggaa gtaaataaga tacgtagtac atatcaataa
      241 aggttaaacg agattaagtt taattatttt cgattgggct taaatttatg tttttttccc
      301 gatgagtgtc aatatgtgaa cggaaaagga attcaatggt taagtcaata tttagaaaaa
      361 ctgaaaatat tgcttttttg ataagaagaa aaagacattt ggtttcataa ggttattggc
      421 aatataacgt tcaattctta cttgcatttt atattggtca ggaactttgc cctgaagtga
      481 taggaatagt cttaatgcgc tgtgtgatgg actagtaaaa aaacaagttt taagggtaag
      541 ggatagaata taaaactata atgagctgtc attgaagatg atttgccaat cagtaagaaa
      601 ttttaatgga ttaacatgcc gcacaaaatg gttataggtg ttcattacaa taagtgatgg
      661 aaaattgaac gggcgtatgt acgtcatctg ttcggtaagc ccccaagagc cagattaagt
      721 gaagttaaac gaaggctgcc agctgtcaca ggaacgatgg cctctttgtc agggcactga
      781 gtgcagttat ctgtgcgcgt tactctaact ggcactcgaa cgtcggcgac ggggttaccg
      841 gtggatcaga ctgttcctgt ggcgtaaacg tttttatgtt agtaccaaca gatttaccac
      901 atttatcatc ttaacagttt gagtgaaaat gcaggctgcg ccgcaaaggg aggagactga
      961 gtggcgtgtt cagtctaaaa gagagttgat gccagcatac aggggcgaag ctggtcaaca
     1021 agtcaacatt aagataatgg aatattcgga gagaaacgtc aggcagttgg cctcaaatga
     1081 acaggaggaa tatatcccca ggaaaattaa tgttggtgtt ataaacacac caacattaat
     1141 ccgatctgat tattaatcag acaaatatcg ttaatccgtc gaattttgaa tcaacgtttt
     1201 ttcttacgac cctgaacggc cttaaaacgt ggattagatt tacaaatcac atatagccgt
     1261 ccttttcgct tcacaatctg acagtctgga tggcgttctt ttgcggtacg cagagagtta
     1321 agaactttca tcacgacccc tttttcgtgc taacaaaacg accaaaacgt tgggtgaatc
     1381 gtgcaacatt tccttctgat gccactgttc tcagcttccc tgtatagaac gggtgcgatt
     1441 tagaagagac atcaattgtc acgtatggat acgttacgcc atccagctca atctcacggt
     1501 ctgttttgat agtcgagccg attttaaagt actcatcaac actggtgtcg tggaacacca
     1561 cagtacgata ctcaggatgg atattgggct tcatcatttt tacctgttat gttataacat
     1621 aaccataaag atacatgctc atccggatca tttcaacacc caatccctgt gactctttgt
     1681 ggatgtggaa ttttcgcaac ggggaactct ggcattgtta gcagccattg gtgacgctcc
     1741 cataaacgct tggatgagtg attgatgatg gttttgctga gagtatggat ttttcgacaa
     1801 tggaggttat ctccacttta ctggccgatg atttatcgca gtatgattga ttacctgaca
     1861 gcgtgtattc ctctcccacg gatataataa ttacttacaa aaagggagag gatgcatatt
     1921 ttaaatatca ctgaagtgaa cagtttattg ccgttattaa tagaaatgga gaaataaata
     1981 ggcgtattct acaattgcga caaaaacaac gatattaatc agtttatgac tgatttgttg
     2041 tactttattc tctttcattg gtacttcctc gctttaaaaa agagcgcact tcgtaagtgc
     2101 ccttatataa ataacgagtt tggtcaacca attttttgac atgtatcaca aatttgaata
     2161 gatgtattac atcggctatc ttttattgca ccaacgtcat tgatatatgt cgcctgaagt
     2221 cagttccggg aatgagtctg atctcaagac tggcccagtc cgggcgttga ttggcgctga
     2281 ggagcatatc gcatctcatc ataatgtcgt atctcctggg gtgttataca agatatcgtt
     2341 gttggtgacc tgggagagga attgagttct attaaaccgt caactatgcc ggatacatac
     2401 tggattacac tgcaggcacg ccttacgaga gaacgtgccg cagtgacggg ttaattatct
     2461 gaaagaattt gtgaggctgt atcggttact cattgat
//
LOCUS       DAPPNJ010000327         2457 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000327 DAPPNJ010000000
VERSION     DAPPNJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.329"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(151..2418)
                     /gene="adiA"
                     /locus_tag="RPM73_004389"
     CDS             complement(151..2418)
                     /gene="adiA"
                     /locus_tag="RPM73_004389"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDX6522644.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          565 a          605 c          672 g          615 t
ORIGIN      
        1 acaggcaaaa aaaagcgcgg ggtggggccg cgcaaaaagt atgacatttt tgtttgcagc
       61 aatttttatg aaaacgttgc catactcaaa aggcctaagg ccaggcggcg taatgttatt
      121 taaacaatta cgccttcagc ggaatagtgg ttacgctttc acgcacataa cgtggtaaat
      181 accgtcaata atttcagtcc cttcagtttc gtgttcaaat ccagggaaat ggtggtccca
      241 ggattgcagc gagcgtaaat aacttacttg cggactgttt ttatcgccga agttttcacc
      301 agacagcagc atcgggattc ctggcggata cgggataact gagtttgccg cgatgcgtcc
      361 tggcagattt tcaatggata ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc
      421 acgcggggtg atttccgcca tcggcaggcc ggaataggct tcgttcaacc gtgcgccagg
      481 gttgttttct ttcagccagg caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc
      541 gtaagtgtca ggatattgtt caacaagttc cggcatcacc tgcgccagcg gtgtgttggc
      601 gtcatagtgg cgtttgaagg agcaaagggt gttaaccaga gtcccccatt tcccacgggt
      661 tacgcccata gagaacagga acataatttg gaagtcagtg gtgcgggtag gcacaatgcc
      721 gtggcgacca agccaggcag tgaccagcgc cgccggaaca ccggtttctt ccagttcacc
      781 atcttcaccc attcccggag caaggatgct gactttaatc gggtcgagca tactccagtt
      841 atccggaata tctttgaagc cgtgccagct ttcgcccgga tgcattaccc agcagtcctg
      901 aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg
      961 gtcggtgacg acttctttgt tccacggttt gaagaaccag ctaccgtcag cggtgaactc
     1021 tttatacagc cgcgccatcg cctgacgaaa atcaaccgct tcgtcaatca cttcttgtgt
     1081 cagtgacagg ccgctgttgc cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat
     1141 ggcatacagc ggggaggtgg tggcatgcat catgtaggcc tggttgaagc gggagaagtt
     1201 aatcgcccca cgaccttcac gtacatgaat ataagaagcc tgtgacagcg cattcagcag
     1261 tttgtgggtg gagtgggtgg cgaaaacggt aggaccgttg tgatcgccag gttcgccgcg
     1321 catggcatag tgatcggcat agatcgggtt gaaacgtgca tagccgtacc aggcttcgtc
     1381 aaagtgcaga cgatcggagg ttttttccag cagatcctgc gcttctttag cgttataaca
     1441 cacgccgtca taggtgcagt tggtcaccac gcagtaagac ggtttttgcc cagctttgtc
     1501 tttagtcagc gggctttcac tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg
     1561 atagattggc ccgataatgc cgtagcggtt gcggcttggc accatgtaga ctggtttcgc
     1621 gcctgtcagc atcaagcctt gttcgatgga tttatggcaa ttacggtcaa cgaccacgac
     1681 atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta gagccggaag taccgacgac
     1741 taccgaccag gagcgatcgg caccaaatac gcgtgcggca tatttttcgc tttcgccaaa
     1801 tgcgccagta tggtcaagca aagaaccgag ggaagttcgt tcgatgccca tatcggtgcg
     1861 gaacagattt tctccatagt agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc
     1921 aacgccgccc tggtggcctg gcgctgccca ggaatattca tggatgtcgc tatatttcat
     1981 cagcgcgctg aacagtggcg gcaacagctg ctggcggtag cgggtcatcg cggcaacagc
     2041 gcgtccggcg ataaagtcgg cggtatcttc cagaatccag gcgaattcat cgacaagctc
     2101 cagcaggtcg cgatccattg cggcgagggc tttttcccga tcgcccaaca ggaagaccgg
     2161 cacgttttgt tggcgctcat gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc
     2221 cggatgttcc atttgataac tgaacatcag gcagtcaatg gcttcgtttg aagagagaat
     2281 ggcaaaacca tcatcaaagg aggtggattt aatcacggta acattttgct ggcttaaagc
     2341 atctgccaga cgctcaacgg cgttaccgac ccaggtgtct tgatggagaa actcgctttc
     2401 aacaattaat actttcatca ttgcttaccc ggttatgaag gaatcttcgt tgcgggc
//
LOCUS       DAPPNJ010000328         2418 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000328 DAPPNJ010000000
VERSION     DAPPNJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.330"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..315
                     /locus_tag="RPM73_004390"
     CDS             <1..315
                     /locus_tag="RPM73_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDX6522645.1"
                     /translation="DYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAIHVA
                     GWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIKSSS
                     AE"
     gene            321..1457
                     /locus_tag="RPM73_004391"
     CDS             321..1457
                     /locus_tag="RPM73_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX6522646.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1454..>2418)
                     /locus_tag="RPM73_004392"
     CDS             complement(1454..>2418)
                     /locus_tag="RPM73_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX6522647.1"
                     /translation="QLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKV
                     GLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGI
                     ITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVA
                     SFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVR
                     DYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLV
                     AIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          592 a          572 c          619 g          635 t
ORIGIN      
        1 gactattttg cactgggagt gcttattttt gtatttctgg tgatcttcta tgggatcatt
       61 attttacatg atattcccta cctgattgcc aaaaaacgta atcatcctca tgccgacgcc
      121 attcatgttg ctggttgggt gagtcttttt acgttacatg ttatctggcc gtttttgtgg
      181 atttgggcca cgctttaccg cccggagcgg ggatggggaa tgcaaagcca tgattcatcc
      241 gttatgcaac tgcaacagcg cattgccggg ctggagaaac agctcgccga catcaaatcc
      301 tcttctgccg agtaatactt atggatttac tgattatttt gacctatgtg gcttttgcat
      361 gggcaatgtt taagatcttc aaaattcccg taaataaatg gaccattccc acagcggccc
      421 tgggtggaat atttattgtc agcggtctaa ttctgttaat gaactataac catccgtata
      481 ccttcaaagc gcaaaaagcg gttatttcta ttcctgttgt cccacaggtg acaggcgtgg
      541 tgatcgaagt gacggataag aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac
      601 tggacccgac gcgttatcag gcgcgggtgg atcggctgat ggcggatatc gttaccgcag
      661 aacataaaca gcgggcgttg ggcgcagagt tagatgagat ggcggcgaat actcagcagg
      721 caaaggccac gcgggataaa ttcgctaaag agtatcagcg ttacgcacgc ggcagccagg
      781 cgaaagtaaa cccgttttca gaacgcgata ttgatgtggc gcggcaaaat tatctggcgc
      841 aggaagcctc cgtaaagtca tcggcggcgg aacaaaaaca gatccagagc cagctggata
      901 gcctggtgtt gggtgaacat tctcaaatcg ccagcctgaa agcgcagctc gcggaagcaa
      961 aatataacct tgagcagacg atagtgcgtg cgccgagcga tggttatgtg acccaggtgc
     1021 tgattcgtcc gggtacctat gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac
     1081 ccgatcagaa acgacaaatc gtggcgcagt tccgtcagaa ctccttgctg cgcctggctc
     1141 ccggcgatga tgcggaagtg gtatttaacg ctctgccagg caaagtgttc agcggtaagc
     1201 tggcagccat tagtccagct gttcccggcg gagcttatca gtcgaccggc accttacaga
     1261 cgttaaacac agcgccgggt tcagatggcg ttatcgcgac cattgaactg gatgagcaca
     1321 ctgatttgag cgctttacca gacggtattt acgcccaggt ggcggtctac tctgatcatt
     1381 tcagccatgt ctcggtgatg cgcaaagtgc tgttacgcat gaccagttgg gtgcattacc
     1441 tttatctcga tcattaatct tcgctttccg ccgcttgttc aggccgcagt tgctgcgact
     1501 gagcaagctg gtggcgaatt tcacgcagca tttcatccag cacatactgg acccgccagg
     1561 gctggtattc ccgtttacgg aagatagcca ccagatccag ccaccattca tacgggtgct
     1621 gaaagcaggg caccagcgtg ccgtcttgca gatcgctctg cacacttttg tctggcgcaa
     1681 acacaatccc caggtgattt cgtgccagct ccagcgccga ttgcgtgtta tcgcaaacgt
     1741 aatttccctt cacgcgataa tcccgcacct ctttactccc ggcgacgttg aagcgccaga
     1801 tattcgcgtc atcaatcatc atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt
     1861 caggacggct aataggatgt ttctcaagat agcgctggct ggcataagcg gttacggcat
     1921 attgggtaat aaaactggca accagcgatt catcttttgg tggagcgtag ctgattaaaa
     1981 catcacaatc atcaggaaat tcgacgcctt cataaaaggc attacgctca agattgcagg
     2041 tttttagcga taaggtgata tcaccgatat ctttaatttt atcgattaca tgtttggata
     2101 aataggtaat tatgccggtt ggggcgtaga tggtgacccg accacgtttc tcatgcttat
     2161 aatctgcaat aaaattatta agctgctcgt ttctgtccag catgtcgttg atgtacggta
     2221 acagcgcggt accaaaaggt gtgagcatca gctgccgggt ggttcggtca aagactttta
     2281 aacccacttt tgattcaaaa tcagcaaggt atttgctgac gttggcctgt gcgatgccaa
     2341 gtacagtcgc ggcatggctg atattttcac tggcagcgat taccgagata atttttaact
     2401 cccggtactt aagttgta
//
LOCUS       DAPPNJ010000329         2412 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000329 DAPPNJ010000000
VERSION     DAPPNJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.331"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..504
                     /locus_tag="RPM73_004393"
     CDS             <1..504
                     /locus_tag="RPM73_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDX6522648.1"
                     /translation="TIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFI
                     LNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFAR
                     WQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAEN
                     RQKRSKK"
     gene            548..>2412
                     /locus_tag="RPM73_004394"
     CDS             548..>2412
                     /locus_tag="RPM73_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDX6522649.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNIS"
BASE COUNT          731 a          546 c          578 g          557 t
ORIGIN      
        1 actattcaaa aacaacaaac tggcataata aacagtacaa agactttaac tcacgtaatt
       61 aaatccgtta aagaccaaca aaatactttt atttttaccg agtttaaccc agcaaaaacg
      121 aagtatttca tcctgaacaa cggttcggtg gctttagcgg gtcgagtgtt atccattgac
      181 gccacagaga atggtagtgt tattcatatc tcactggtca acttattaag tacacctatc
      241 tcaaacattg gttttaatgc gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt
      301 gcccggtggc aacaattact tttcaacaca tcaatgaaat ccacattgaa attattacca
      361 ggtcaatggc aagacattaa tttgaccctg aagggtgtat cgcccaataa cctgggatat
      421 ctgaagttag ccatcaacat ggaaaatatt cagttcgaca atcttccctc tgctgaaaat
      481 cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct tataacaagg aatagcgagc
      541 atcattaatg aacaaaatat ttaaagttat ctggaaccct gcgacaggga attatactgt
      601 taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc
      661 tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga
      721 caacggtcag ggtgttgatt acggtagtgg atcagctggc gacggctggg ttgctatagg
      781 caaaggggcg aaagcaaata cttttatgaa caccagtggt tccagtactg ctgtgggtta
      841 tgacgctata gctgaaggcc aatatagctc tgccatcggg tcaaaaaccc atgcgattgg
      901 cggtgcatca atggcctttg gggttagtgc aatatcagaa ggcgatagaa gtatagcact
      961 gggtgcctct tcgtattcat tgggccaata ctcaatggcc ctcggccgtt attcaaaagc
     1021 attgggtaaa ttgtctattg ctatggggga ctcttccaaa gcggaaggag caaacgccat
     1081 tgccctggga aatgccacta aagctactga gattatgagt attgctcttg gcgacaccgc
     1141 caatgcgtca aaagcgtatt caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa
     1201 cgcaattgcc ctggggcgta gcagtgtagc tagcggtact gacagcctcg catttggcag
     1261 acaatcactt gccagcgcag cgaacgctat tgcgataggt gctgagaccg aagccgctga
     1321 aaatgcaact gctattggca ataatgcgaa ggcaaaaggg actaatagca tggcaatggg
     1381 gttcggaagc cttgccgata aagtcaatac tatcgcatta ggaaatggca gccaggctct
     1441 ggcagataat gcaatcgcca taggccaggg caacaaagct gatggcgtgg atgccatcgc
     1501 tctgggtaat ggtagccagt cgagaggctt aaacaccatt gccttaggca cagccagtaa
     1561 tgcaactggt gataagagtc ttgcgcttgg tagtaatagc agtgccaacg gtattaactc
     1621 tgtcgcgctg ggcgcagatt ccattgcgga tttagacaat accgtctctg tcggcaatag
     1681 ttcattaaaa cgcaagatcg ttaatgtgaa aaatggcgcg atcaagtctg acagttacga
     1741 tgccattaat ggttcacagc tttatgccat tagcgactcg gtagcaaaaa ggcttggagg
     1801 aggggctgca gtagatgttg atgacggtac tgttacagca ccaacctaca atttaaaaaa
     1861 tggtagcaaa aataacgtag gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg
     1921 ggaccaaacc aaaggcaaat acagcgctgc tcatggtact agtagcccaa ctgccagcgt
     1981 aatcaccgat gttgcggatg gcacgatttc agcctccagt aaggatgcgg ttaacggttc
     2041 ccaactgaaa gctaccaatg acgatgtcga agccaacacc gccaatatcg ctactaatac
     2101 cagcaacatt gccacgaata cggcaaatat tgccaccaat accaccaata tcaccaacct
     2161 gacggattcc gttggtgacc ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc
     2221 attcagtgca gctcacggcc aggataccac cagcaaaatc accaacgtta aagatgccga
     2281 cctgacggct gacagcactg atgctgttaa cggctctcag ctgaaaacca ccaacgatgc
     2341 tgtggcgacg aataccacca atatcgccaa taacacttcc aatattgcca ctaacaccac
     2401 caacatctct aa
//
LOCUS       DAPPNJ010000330         2387 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000330 DAPPNJ010000000
VERSION     DAPPNJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.332"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            273..416
                     /locus_tag="RPM73_004395"
     CDS             273..416
                     /locus_tag="RPM73_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDX6522650.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            429..638
                     /gene="cspE"
                     /locus_tag="RPM73_004396"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             429..638
                     /gene="cspE"
                     /locus_tag="RPM73_004396"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDX6522651.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            804..1613
                     /gene="rlmA"
                     /locus_tag="RPM73_004397"
     CDS             804..1613
                     /gene="rlmA"
                     /locus_tag="RPM73_004397"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDX6522652.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1610..2176)
                     /gene="mntP"
                     /locus_tag="RPM73_004398"
     CDS             complement(1610..2176)
                     /gene="mntP"
                     /locus_tag="RPM73_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDX6522653.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          624 a          583 c          588 g          592 t
ORIGIN      
        1 tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt attgaactat
       61 gttagaaact acgccagttt aagtatctgc ctgaactggc aaggttaagc acaatgatat
      121 atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt
      181 gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta tgtgataaca
      241 gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt aaagaagtcc
      301 tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt ggtgcctcat
      361 gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca atctaaggaa
      421 tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct aaaggttttg
      481 gcttcattac tccggctgat ggcagcaaag acgtgttcgt acacttctcc gctatccagg
      541 gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt caggacggcc
      601 agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg atctgaagtg
      661 tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt actatgcttt
      721 atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc
      781 tttagtatcc tgccggagtt gtcatgtcgt tttcctgtcc actttgccat cagcctcttt
      841 cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg gcgaaagaag
      901 ggtatgtcaa tctgctgccc gttcagcata aacggtctcg tgatccgggc gacagcgcgg
      961 aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg ctgcgtgatg
     1021 caattgtcgc ccaactgcgg gaacggcttg atgaaaaggc cacggcagtg ctggatattg
     1081 gctgtggtga agggtattac acacacgcat ttgccgatgc gttgcccgaa atcaccacgt
     1141 ttggcctgga tgtctcgaag gtagcgataa aagcggcggc gaaacgctat ccgcaggtca
     1201 ctctttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg gacgccataa
     1261 tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag cccggcggct
     1321 gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg ctgatttaca
     1381 atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca ttacagcaga
     1441 gtgatgagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca ttattgcaga
     1501 tgacgccgtt tgcctggcgt gcgaagccag aagtctggca aacactggca gcaaaagaag
     1561 tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat taaccgtgga
     1621 agtgcgtcca gaggatctgg acgccgatgc cgatcagtac cagcccgccg agaatttccg
     1681 cttttttccc aataatcgag ccgataaagc gaccaaccat catccctaat gtcgacataa
     1741 tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc aggaaagcaa
     1801 gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt accagtagcc
     1861 agaaaccgtg ccgacggcgc ggctcttcat cttcatcatc tgcgccacga aaaccctcaa
     1921 taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtga ttccattcaa
     1981 ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc agggtttcga
     2041 cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta tggagggtgg
     2101 cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca agaagaacag
     2161 tagcagtgat attcatgaca acgtcctgac cggggtatcc ataaaacaca tcatcgcccc
     2221 cagcaaacat gcatacatga acaacaagcc gcgatgggtt ttcgcgttca tgcaggcata
     2281 gcgattgatg tgcatatggt ctcgcctgac tgaaagtctt cagtccgtac gtgccacgtt
     2341 ttgcaacgag tatgttgaca cgtacatttc cggaatctgg aaatcag
//
LOCUS       DAPPNJ010000331         2380 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000331 DAPPNJ010000000
VERSION     DAPPNJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.333"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..429
                     /locus_tag="RPM73_004399"
     CDS             <1..429
                     /locus_tag="RPM73_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDX6522654.1"
                     /translation="AKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDA
                     GYQTEVMIKTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFA
                     SNLSDKFAVYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(465..1256)
                     /locus_tag="RPM73_004400"
     CDS             complement(465..1256)
                     /locus_tag="RPM73_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDX6522655.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1262..2320)
                     /locus_tag="RPM73_004401"
     CDS             complement(1262..2320)
                     /locus_tag="RPM73_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDX6522656.1"
                     /translation="MLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRRFQPDWVE
                     KYCLVADLSEIEAGALYMDGILQEILPPSTRRLYWRVEDDLTLVRMNTQQVQVQTEVM
                     NAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDGETQALLPPGLTAYWKINHLVDAE
                     VVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQFA
                     LREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILA
                     QLVEAEKSAQANVIRRREETAATRSLLNTAKVMENNPVALRLKELETLERVAERIDNI
                     SVFGGLDQVLHGLVNIKG"
BASE COUNT          609 a          676 c          517 g          578 t
ORIGIN      
        1 gcgaaatttt ccggcgaaac ggtcgaacgt gcaattgaga gagcgatagt cagtaaactc
       61 aacattgttg tcgaaggaac atttcgtaat gccgcgacgc cgttacaaac attaaaaaaa
      121 ctcaaagatg caggctacca aacagaagtc atgataaaaa caacctcagc ggcattaagc
      181 tgggaaagca ccaatgaacg gtatagtaag gacaaagagg cgggaaatat tgcccgaaaa
      241 gtggataaaa accaccatga tatcgtcacc ggtttgctgg cagaaaatgc ccgcaaggtg
      301 tttgcttcga acctgtcaga taagtttgcg gtgtattcgc gtgagaagat gatatttagt
      361 tcacaagcgg caacgaatga cgatattgca acacttatac agaatgaaat cagcgggaat
      421 acacaataat aataagagct gatggcgtga aataacgccg ggcatcacgc ccggcgcatt
      481 accgtctggt acattaagct atccagttcc ttaacgtccc cttctctgct atccggtaac
      541 agcggtggaa tttccccacg cgcctgttcc gcacgaataa acgcatgtaa caatggacgg
      601 cgcggaccat attccgcttc tcctgcgcgc tgtttacgtt ccagtaattc gtctatttct
      661 gcacgtaacg gcgcatcaag ttcgcttccc gccagtaact cagcaaagcg cattggcggt
      721 acgcctttac ccgcttcgac ccagcgcacg gccagcagcg gacgcagcac gtaaaagtat
      781 tttttcagtc gaacttcgtc gccctgcaaa tatccgggga agtttttctg cgccatcgaa
      841 tagtaatgcc agcgcgcacg cagcggagaa aaccacattg ggaccattgc cttaaacgcc
      901 gttatcgttt cttcatcttg ttgataaatc accggagaat ccagccattc tatcagcgtc
      961 gggttcgcgc ctttcaataa tcccagcgcc ttacgccact cccaaccgga aacatctaac
     1021 tcgtcgtcga tgggcagctc aataacatcg cgcggtgact ctactcgcag ataccattcc
     1081 agcgggtgaa catacaaaaa ccgtacatcg tagtcgctgt cgggcgaggc aaatccccag
     1141 ccgcggctcc ctgattcaca ggcgtacagt actttcacat cataccgccg ttcaatttct
     1201 tttaactgct gcgataccct ttcgcgcatc gcagcactga ctccatttaa tggcatgtta
     1261 ctcacccttt gatattaacc aacccatgca gaacctgatc cagaccgcca aatacagaga
     1321 tgttatcgat ccgctcagcc actctctcca gcgtttctag ctcttttaaa cgcaacgcta
     1381 ccggattgtt ctccatgact ttggcggtgt tcagtaacga gcgcgtcgct gccgtttctt
     1441 cacgtcggcg aatcacattc gcctgcgcag atttctctgc ctcaaccagc tgcgcgagga
     1501 tatttttcat atcaccaggc aggacgatat ctttcacccc taatgaggca atctccatac
     1561 caaatggcaa catgcgcgat ttcacctgtt cgctgacaac gtcatcaatg acctgtttat
     1621 cttccagcag ttcatccagc gtacgagtac ctaccgcttc acgcagggca aattgcaatt
     1681 cccgatacaa atgatcaatc ggcttcgtta actgactaaa tgccagcaac acgtcgctgt
     1741 agcgccagtt agcggcgagg ttaatccgca gattgacctt atctttcgtt aagatttctt
     1801 gcccgctgac ttccagtacc tgcaagcggg tgtcaaccac ttcagcgtca accagatggt
     1861 taattttcca gtatgcggtc agccccggcg gtaacagcgc ctgcgtttca ccatcgattt
     1921 tcagcacgcc tacatgccag gccggaacct gcaccgttag tattgcatcg cggcctttaa
     1981 ctgccccttt gcgccgtggt tgcaataccg cattcatcac ctccgtttgt acctggactt
     2041 gctgcgtatt catccgcacc agtgtcaaat catcctctac acgccagtac aaacggcgcg
     2101 ttgatggcgg caggatctcc tgcaaaatac cgtccatata caacgccccg gcttcaatct
     2161 ccgacaagtc tgccaccagg caatatttct ccacccaatc aggctgaaag cggcgcaaat
     2221 aatccgccag cacgtcggga acttcgctgc catcgagcgt aatcagcaag acttctggcg
     2281 tatttaacca gggtaaaaga tgctcgcctg catgtagcac tttgtagtaa tcgccgtttc
     2341 tggagagtaa tgccagctga tcttttctta cgcttatttt
//
LOCUS       DAPPNJ010000332         2373 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000332 DAPPNJ010000000
VERSION     DAPPNJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.334"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            34..522
                     /gene="eco"
                     /locus_tag="RPM73_004402"
     CDS             34..522
                     /gene="eco"
                     /locus_tag="RPM73_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDX6522657.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(671..2317)
                     /gene="mqo"
                     /locus_tag="RPM73_004403"
     CDS             complement(671..2317)
                     /gene="mqo"
                     /locus_tag="RPM73_004403"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDX6522658.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          552 a          657 c          599 g          565 t
ORIGIN      
        1 gaaaacctat aaatgaagaa ggaaagcaaa aaaatgaaga ccattctacc tgcagtattg
       61 tttgccgctt tcgctaccac ttccgcctgg gcggcagaaa gcgtccagcc actggaaaaa
      121 atcgcgcctt atccacaagc tgaaaaaggg atgaagcgtc aggtgattca gttaaccccg
      181 caagaagatg aatctaccct gaaagtagaa ctgttaatcg gtcagacgct ggaagtcgat
      241 tgcaatttgc atcgtctcgg cgggaagctg gaaagcaaaa cgctggaagg ctggggctat
      301 gattattatg tctttgataa agttagctcg ccggtttcca cgatgatggc ctgcccggat
      361 ggcaagaaag agaagaaatt tgtcaccgcg tatctgggcg atgctggaat gctgcgttac
      421 aacagcaagc tgccgatcgt ggtgtatacg ccagacaatg tagatgtgaa gtaccgcgtc
      481 tggaaggcgg aagagaaaat tgacaacgcg gtagttcgct aaaatgctgt gaagtgcggc
      541 accccgtagg ccgaataagg cggtcacgcc gcatccgaca tctaacgccc gagccggtgc
      601 ctgatgcgac gctggcgcgt cttatcagca tacgccacat ccggcatacc atgccggatg
      661 tggcacatca ttacaacgca atatccgcca cttctttttg aacgggttgc ggtttcaact
      721 gcggttttgg cgtactatct gctgcctgcg gcttgtcgta tttcagcccc agcacttcgc
      781 tggtgtactg caactcgcgt tctgttgccg ctacatcacc gttcagcttg cgtccataag
      841 acggaacgat cgctttcaac gtagcctgcc attgcgggct ggaaacgcga tcgccaaata
      901 ctttttccag cagatccaac ataatcggcg cggcggttga cgcccctggc gatgccccca
      961 gcagcgcggc aatggtccct tgctggtcac tgaccacttc agtacccaga cgcagtacgc
     1021 cacctttctc ggcatcacgc ttgataatct gcacgcgctg ccccgcttgc cacaaacgcc
     1081 agtcctcttt ttttgcctgc ggatagtact ctttcaacgc ttcaaaacga tcctcttcac
     1141 tcaacatcac ctgactcacc agatatttca ccagatcgaa attatccagc ccgacgtgca
     1201 tcatcggcat cacgttagag gtggtggtgg aactcatcag atcccacaat gaaccgtttt
     1261 tgaggaattt ggttgagaag gtggcaaatg gcccaaacag cactacgcgt ttaccgtcca
     1321 gaacgcgggt atcgatatgc ggaaccgaca tcggtggtgc gccaacggat gctttaccgt
     1381 aaaccttcgc cagatggtga ttaaccacgt ccgggttttc cgaaacaagg aactgtccgc
     1441 ccaccgggaa accggcgtag tctttcgctt ccggaatccc cgattcctgt aatagcttca
     1501 gcgccgcacc gcccgcgccg ataaagacaa atttggcgcg gatgttctgt gcagtgccgt
     1561 ttttcagatc ggcaacggta acggtccagg tgttatcgtc attacgcttt agggcgcgga
     1621 cttcgctgct gagttgcagc gagaagttag atttcttctg taaggaagca attaactggc
     1681 gggtgatctc gccatagttc acatcagtgc caatttctgt gcgtgttgcc gctactttct
     1741 gttgtggatc gcgcccttcc atcactaacg gtgcccactc tttgatctgc gcgtgatctt
     1801 cagagtaacg cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca
     1861 ggaaattaac gttatcctcg ccccagacaa agctcatatg cggaacggta ttgataaatg
     1921 aacgcggagt acgcagcacg ccgcgctcaa cctggtgcgc ccagaactgg cgggaaatct
     1981 gaaatgcttc gttaatggcg actgcttttt caatactgat gctgccatcg gcgttttgcg
     2041 gggtgtagtt cagttccatc agtgcagaat gcccggttcc ggcgttattc cagccgttcg
     2101 aactctcctg cgcgacaccc tccaggcgct ccaccatggt catcgaccat tcaggctcca
     2161 gctcgcgtaa ataggtcccc aacgtggcgc tcataatgcc gccgccaatc aacagtacat
     2221 cagtttcctg ctcctcggac gctttcgctt ttgccgccat cgaaacggca ttaagcccca
     2281 cggccatcga gaagagcatg gcagtcactt ttttcatctt gttaatgcct tacttttagt
     2341 cgctttattg caggtgagat ttgcgcggca tca
//
LOCUS       DAPPNJ010000333         2286 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000333 DAPPNJ010000000
VERSION     DAPPNJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.335"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            30..533
                     /locus_tag="RPM73_004404"
     CDS             30..533
                     /locus_tag="RPM73_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX6522659.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(579..995)
                     /gene="rraB"
                     /locus_tag="RPM73_004405"
     CDS             complement(579..995)
                     /gene="rraB"
                     /locus_tag="RPM73_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDX6522660.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1157..2161
                     /gene="argF"
                     /locus_tag="RPM73_004406"
     CDS             1157..2161
                     /gene="argF"
                     /locus_tag="RPM73_004406"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDX6522661.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
BASE COUNT          571 a          550 c          576 g          589 t
ORIGIN      
        1 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
       61 tgcgtcgcct gacgttgcag gataatcctg ctatcgcccg cgtcattcgt caggtatccg
      121 ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaac cttgacgagt
      181 tgtatcaagt gtatagtcag cctggccatg catattgggt ggttgagtac gatggtgaag
      241 tggtcggcgg cggtgggata gcgccattag caggtagtga atcggacatt tgcgaactgc
      301 aaaagatgta ttttcttccg gctattcgcg gtaaagggct agcgaaaaaa ctggccttaa
      361 tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
      421 ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc
      481 ttggctgcac gggccatgtc gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
      541 cccgctagtg tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      601 tcttcgtcga caaaatcttc atcgtcgccg tcttcgccgt tgggatcttc aaagtaagtg
      661 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
      721 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
      781 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
      841 tcaactgctg ctttttccag ggtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
      901 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgcgtttct
      961 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1021 gatactccta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1081 tttagacttg caaatgaata accatccata taaattgaat tttaattcat tgaggcgata
     1141 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1201 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     1261 aagcggtaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1321 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccaggggg ctcgcgttac
     1381 ttatcttggc ccaagcggca gccagattgg tcataaagag tcgattaaag acactgcccg
     1441 cgtgctgggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgttga
     1501 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1561 cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttgcccggca aagcgttcaa
     1621 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     1681 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
     1741 agctgcgctg gttacggaat gccgcgccct ggcacagcaa aatggcggga atattacgct
     1801 gacggaagat gtagccaaag gagtagaagg tgctgacttt atctataccg atgtgtgggt
     1861 gtcgatgggg gaagcaaaag agaaatgggc agaacggatt gcattactgc gtgattatca
     1921 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccattgcct
     1981 gcccgcgttt catgacgacc aaacgacgct tggtaagaaa atggcggagg agtttggcct
     2041 acatggcggt atggaagtca ctgatgaggt cttcgaatct gccgccagca ttgtttttga
     2101 tcaggcggaa aaccgtatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaacta
     2161 attgatatcg ctataaagat gccgggaaat acaaaactat cccggcatca cattatttta
     2221 tgcagtgata gttctaatct tccacccaaa tttttctatt gcataacgta aatcttccgg
     2281 tggatt
//
LOCUS       DAPPNJ010000334         2258 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000334 DAPPNJ010000000
VERSION     DAPPNJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.336"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            37..450
                     /gene="hiuH"
                     /locus_tag="RPM73_004407"
     CDS             37..450
                     /gene="hiuH"
                     /locus_tag="RPM73_004407"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDX6522662.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            559..1563
                     /gene="msrP"
                     /locus_tag="RPM73_004408"
     CDS             559..1563
                     /gene="msrP"
                     /locus_tag="RPM73_004408"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDX6522663.1"
                     /translation="MKKKQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1564..2199
                     /gene="msrQ"
                     /locus_tag="RPM73_004409"
     CDS             1564..2199
                     /gene="msrQ"
                     /locus_tag="RPM73_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDX6522664.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
BASE COUNT          576 a          556 c          548 g          578 t
ORIGIN      
        1 atcctcaaaa ctcattcaca tgacaaggat ataaacatgt taaagcgtta tttagtactc
       61 tccgtagtaa cggcagcatt ttcattacct tctttggttt atgccgcaca acaaaacatt
      121 cttagcgtgc acattttgaa ccagcaaacc ggaaaacccg ctgccgacgt gacagtcact
      181 cttgaaaaga aagcggacaa cggctggtta caacttaata ccgccaaaac agataaggat
      241 ggacgaatta aggcactgtg gcccgagcaa actgcaacta cgggcgatta ccgtgtcgta
      301 tttaaaaccg gggactattt caagaaacaa aatcttgaaa gtttcttccc tgagatcccc
      361 gttgaatttc atattaataa agtgaacgag cattatcatg tgcctttatt acttagccaa
      421 tatgggtatt caacctatcg tggcagttaa tgcataaata tgtaaaagca gatctctgct
      481 actgttaggg aggacactcc ctgacagatt aacagtaaac ggctcttgct ggctaacgac
      541 aaaaaagtgt gatggcttat gaaaaagaag caatttttaa aagaatcaga tgttacggcc
      601 gagtcggtat tctttatgaa gcgtcgacag gtgttaaaag cactgggcat cagcgcagct
      661 gcactttctt tgcctcacgc tgcgcatgcc gatctgctta gctggtttaa agggaacgat
      721 cgcccgcccg cccccgccgg aaaaccgctg gagttcagca agcctgccgc ctggcaaaat
      781 aacctgccac tgacgccagc agataaagtt tccggctata acaacttcta tgaattcggg
      841 ctggataaag ccgatcccgc cgctaatgct ggtagcctga aaaccgatcc atggacactg
      901 aaaatcagcg gcgaagtggc aaaaccattg accctcgatc acgatgattt aacccgtcgc
      961 ttcccgctgg aagagcgtat ttatcgtatg cgctgcgtgg aagcgtggtc gatggtggtg
     1021 ccgtggattg gttttccgct gcacaaattg ctggcgcttg ccgaacccac cagcaatgcg
     1081 aagtatgtcg ctttcgaaac aatttatgca ccggaacaga tgcctggcca gcaggaccgc
     1141 tttatcggcg gcgggctgaa atatccttat gtcgaaggat tgcgtctcga cgaagcaatg
     1201 catccgctca cactgatgac cgtgggtgtt tatggcaagg cgttaccgcc acaaaatggc
     1261 gcgccggtac gactgattgt gccgtggaaa tatggcttta aagggattaa atcgatagtc
     1321 agtattaagc tgacccgcga gcgtccgcca accacctgga atctggcagc gcctgacgaa
     1381 tacggttttt acgccaacgt taatccgcat gttgatcacc cgcgctggtc acaggctacc
     1441 gaacgattta ttggttcagg cggcatcctc gatgtacagc gccagccaac gctactgttt
     1501 aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc tggatttgcg ggagaatttc
     1561 tgaatgcgtc tgacagcaaa acaggtgaca tggctgaaag tttgcctgca tcttgccgga
     1621 ttgttgccgt ttctctggct ggtctgggcg atcaatcacg gtggactggg tgccgatccg
     1681 gtgaaagata ttcagcattt taccggtcgc actgcactga aatttttgct ggcgaccttg
     1741 ttaatcacac ctctggcacg ctacgcaaaa cagccgttat tgatacgcac tcgccgcctg
     1801 ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa ccagttacgc attgctggag
     1861 ctgggcgtga ataatctggc gttattaggt aaggagttaa tcacccggcc ttatttaacg
     1921 ttaggtatta tcagctgggt aattttgctt gctttagcgt ttacttcaac ccaggcgatg
     1981 cagcgaaaac tcggcaagca ctggcaacag ttgcataatt tcgtctatct tgtcgcgatc
     2041 ctggccccaa tacattatct gtggtcagtg aagattattt caccgcagcc gctcatctac
     2101 gctgggctgg ctgtactgct tttagcctta cggtataaga agttgcgttc cctttttaac
     2161 cggttacgca agcaggttca caacaaatta tctgtataac ttcatattgc cgacaaagta
     2221 cgcgttatcc ctgccggatg tggcgcgagc gccctatc
//
LOCUS       DAPPNJ010000335         2108 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000335 DAPPNJ010000000
VERSION     DAPPNJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.337"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..521)
                     /locus_tag="RPM73_004410"
     CDS             complement(<1..521)
                     /locus_tag="RPM73_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDX6522665.1"
                     /translation="MGVVKLADYRHNPVQHQEASSMGYVSIHRQFMDSRLYKDSQAVH
                     LWLHLILKANHESTVVNTDIGPITVDRGQMITGRPSLVRETFIPDNKVRSLLRTFESK
                     GMLNICSMGKKFSLFTIVKYDDFQAKNCPTVVQRLSNANTSNGAALSGDCPTVVQRLS
                     INNNINNISNTDV"
     gene            complement(508..669)
                     /locus_tag="RPM73_004411"
     CDS             complement(508..669)
                     /locus_tag="RPM73_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522666.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNKAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(702..998)
                     /locus_tag="RPM73_004412"
     CDS             complement(702..998)
                     /locus_tag="RPM73_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522667.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRSLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(1108..1323)
                     /locus_tag="RPM73_004413"
     CDS             complement(1108..1323)
                     /locus_tag="RPM73_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6522668.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            1441..2094
                     /locus_tag="RPM73_004414"
     CDS             1441..2094
                     /locus_tag="RPM73_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDX6522669.1"
                     /translation="MKWYELARSRMKELGITQEKLAEELGMTQGGIGHWLRGSRHPSL
                     DEIGVVFKYLGIDNVSFNHDGTFSPAGEYSSAPVKKQYEYPVFSHVQAGMFSPELRTF
                     TKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGMLILVDPEQAVEPGDF
                     CIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVGKVIASQWPEETFS"
BASE COUNT          554 a          469 c          475 g          610 t
ORIGIN      
        1 aatacgtcag tattagagat attatttata ttattgttta tggacaaccg ttggacaacc
       61 gttggacaat ctccgctgag agccgcgcca ttactggtgt ttgcgttgga caaccgttgg
      121 acaaccgttg gacaattttt tgcctgaaaa tcgtcatatt taacgattgt aaacaggcta
      181 aatttcttcc ccatcgagca aatattaagc atccctttcg actcaaaagt ccgtaataag
      241 ctccgaactt tgttgtcggg gatgaatgtt tctctgacca gcgacgggcg tccagttatc
      301 atctgaccgc gatcaacagt tatcggaccg atatccgtat tgacgacagt agattcgtga
      361 ttagccttga ggattaagtg aagccaaaga tgtactgcct gagagtcctt atagagcctg
      421 ctgtccataa actggcggtg tatagagaca taccccatac tggatgcctc ctgatgttgt
      481 acagggttat gcctgtaatc agctaactta acgacgccca tgtttcactc ctgctttggc
      541 tagtctgtaa acaccaacaa ggcgctctgc gaacgccttg ttatttgctg cggctaccac
      601 taatccctca ggtgaatcag ggtgtcgaat ctcttctttt tcctggtatt tcttacgacg
      661 ttttgtcata attactcctg tggattgatc cagtaattcc ctcagaattg catatcaatt
      721 tgcttaaaat cctcggtggc ggccggggat tttttctttg tgatttcatc aagcgcatac
      781 ttaaaagccc tgctaatcgg actgatatct gatgccattc cgaaagcaca caagaccgag
      841 gcaataaatc tccagtccgt tctgcttatc ttcgattcat gacagccaat catctttgcc
      901 agactgcgct gggtaagtgt tgacaggttg atgagtaaat cggtttctgc gcgatcaaca
      961 tcgcgctgtg atagtttgct gtaacttgtt tgttccattt cttactattt ccataggtaa
     1021 ataatcacta atactcatct ttcgatgagc gattaattag ttaccgcgtt gtcggcggtg
     1081 cagattgata aagagcggat ccgcttatta agcggctttg tgttccggcg ggaacacgtc
     1141 atcaagactg acttttgcgc ctaacttgtt taggcacgca acaagagcac ggcatgtttt
     1201 aaggtctggg aagcgacgac cagattccca atgtccgata gctccctgtg tgcatccaac
     1261 cgccttagca agtgttgttt gagagatatt cagtgactct cgatattttc gtaggttgct
     1321 catatgccct ccatagtaac catgaaacaa taatacgata tgtacttttg gaatgcaaac
     1381 aaaaaataca tcttgtgcat ggatggtttt agtacatagc gtaataataa ggatatgaaa
     1441 atgaaatggt atgaactggc tagatccaga atgaaagagc tcggcataac tcaagagaag
     1501 ttagctgaag agcttggtat gacgcagggt ggaattggtc actggttgcg cggatctcgt
     1561 catccatctc ttgacgagat tggtgtggtg tttaaatacc ttggtattga taacgtctca
     1621 ttcaaccacg acggtacatt ttcacctgct ggcgaatact catctgcccc cgttaaaaaa
     1681 caatatgagt accctgtttt ttctcatgtt caggccggga tgttctcgcc tgagcttaga
     1741 acctttacca aaggtgatgc ggagagatgg gtcagcacaa ccaaaaaagc cagtgattgt
     1801 gcgttttggc ttgaagttga aggtaattcc atgaccgcac caacaggatc caagccaagc
     1861 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggagatttc
     1921 tgcatagcca gacttggtgg tgacgagttt accttcaaga aactgatcag ggatagcggt
     1981 caggtgttcc tacaaccact aaacccgcaa tatccaatga tcccatgcaa tgatagctgt
     2041 tccgtagtag ggaaagttat cgccagccag tggcctgaag agacatttag ttaacagcct
     2101 caccactc
//
LOCUS       DAPPNJ010000336         2103 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000336 DAPPNJ010000000
VERSION     DAPPNJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.338"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(63..1109)
                     /gene="aroH"
                     /locus_tag="RPM73_004415"
     CDS             complement(63..1109)
                     /gene="aroH"
                     /locus_tag="RPM73_004415"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDX6522670.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1266..2099)
                     /gene="ppsR"
                     /locus_tag="RPM73_004416"
     CDS             complement(1266..2099)
                     /gene="ppsR"
                     /locus_tag="RPM73_004416"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDX6522671.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          513 a          531 c          552 g          507 t
ORIGIN      
        1 aatgtgctga gaagacaaca agttgcgcag aaatgcccat cccatcagga atgggcacgc
       61 attcagaagc gggtatctac cgcagaggca agtttttcga ccaggcgttc ggtatcttcc
      121 cagcccagac acgggtcggt aatggattga ccgtaagtga gcggctgacc gccgacgatt
      181 ttttgcgttc cttcgcgcag gaaactttcc gccataattc cagcaatcgc cgtagagcca
      241 ttgcggattt gctgacaaat atcctcacaa acttctaact ggcgacggtg ctgcttctgg
      301 cagttaccgt ggctgaaatc caccaccaga tgttcaggta aatcaaactc gtgcagcgta
      361 tcgcaggctg cggcgatatc atcggcatga taattcggtt ttttgccgcc acgcataata
      421 atgtggccat acgggttgcc gctggtctga tagatggtca tctgaccatt tttgtctggc
      481 gagagaaaca tatggctggc gcgggctgcg cggatagcat ccacggcaat ccgcgtgttg
      541 ccatcggtac catttttaaa acctaccgga caggagagtg ccgaagccat ttcacggtgg
      601 atctgacttt cggtagtacg tgcgccaatc gcgccccaac tgattaaatc agcaataaac
      661 tgaccggtca ccatatcgag gaactcagtc gcggtaggga cacccagctc atttacctgt
      721 aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc gatagctgcc gtttaaatcc
      781 ggatcggaga ttagcccttt ccagccgaca acagttcgtg gtttttcaaa ataggtgcgc
      841 attacgattt ccagccgtga ctggtactgg ttgcgcagcg actgcagacg ggtggcgtac
      901 tccattgcag cggtgagatc gtggatcgag caggggccaa taataaccaa cagtcgctta
      961 tcttcaccat tcagtatttt ttcaattctg cggcgggagt cggtgacatg ggtggcgacg
     1021 ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg ttaccaggct ctcaatacgc
     1081 gcagtacgga gttcgtcagt tctgttcatt acaagtctca gaaatttgtt gcttctacgg
     1141 caggactacc gggaagtgat gcgcatcacg ataaaccaat cgcagctgat ttcaagtgcg
     1201 aggtgtgtca cacctcgccg ggtggttagc atgataacaa aaaaataagc taatgcacta
     1261 gttctctagt acattcggcg gctaagcccc atgatatcga ggatcttggt ggcaatctct
     1321 tctaccgaat aattggtact gttaatccac gggatctgat ttttacggta caacgcttct
     1381 acttccgcga cttccatcct gcactgacga agcgaggcat agcggctgtt ctcccgacgt
     1441 tcctcgcgaa tcgccgccag acgttccggg tcgatagtca ggccgaacaa tttatgctga
     1501 agcggtttga gcgacgcggg tagcaccaga ttatccatat cgtcggcaat aaaggggtag
     1561 tttgccgcgc ggataccaaa ttgcattgcc agatacagac tggtgggggt tttaccacag
     1621 cgagaaacgc cgagcaggat cacctgagcc tggtccagat tgcgcaacga aataccgtca
     1681 tcgtgggcaa gggtgtaatc aatcgccgca atgcgcgcat catatttatt gagattatta
     1741 gggttaaggc catgggtacg atgagcaatc ggcgttggat ccagtttcat ctcttgttgt
     1801 agcggggcaa ccagcgcctg aacgatatcc tggcaaaagc cttcactttg caagatgatg
     1861 gcgcgaatct ccggcaacac gatggagtag aagaccagcg ggcgcacacc tgtctggtga
     1921 taaatggcgt caatctgatc cttcactgcc cgtgcacggc tctcattttc gacaaacggc
     1981 agcgtaatgc tgctgatagt gacgggaaat tgtgacatta ctgcgtgtcc taatacctcc
     2041 gcagttattg ccgtaccatc agaaatataa aaaacgtggc gatcaacagc attatccatt
     2101 ttg
//
LOCUS       DAPPNJ010000337         2088 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000337 DAPPNJ010000000
VERSION     DAPPNJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.339"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(64..429)
                     /locus_tag="RPM73_004417"
     CDS             complement(64..429)
                     /locus_tag="RPM73_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522672.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     HVGKVFDGVLSPAQPLIRI"
     gene            complement(429..548)
                     /locus_tag="RPM73_004418"
                     /pseudo
     CDS             complement(429..548)
                     /locus_tag="RPM73_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(561..>2088)
                     /locus_tag="RPM73_004419"
     CDS             complement(561..>2088)
                     /locus_tag="RPM73_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522673.1"
                     /translation="SESVFNILNSTGAATDKSYLSLNPDKYPNYRLLIHSAKLQNEIK
                     SHYTKDEIQGLLTLTENTRKLTLTEKPWGTFILASTFEDDKTAAETHYDAVWLRDSLW
                     GYMALVSDQGNSVAAKKVLLTLWDYMSTPDQIKRMQDVISNPKRLDGVPGQMNVVHIR
                     FDSNSPVMADVQEEGKPQLWNHKQNDALGLYLDLLIQAIDTDTINAEDWQKGDRLKSV
                     ALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSNLLSKKDSVFVSDLL
                     REAKANELDEPLSTTRLNHLIDKGYERITLQLDLGGESPGYLEKDKHYREADAALLNV
                     IYPANLAKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQSANFWFNDIKTDTDQNSHA
                     KREKSFIPSTEAEWFFDSWYAKSAAIVYKESRKEEYLNDSVQFMNRSLAQITGENMIG
                     ANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASMTLMLKEMSNLINDEGIK
                     "
BASE COUNT          535 a          462 c          379 g          712 t
ORIGIN      
        1 cactggagcg tttccgtatt ggcagactgt gactttatct aaccaaagat tcaattcccc
       61 tgttcatatt cttattagtg gttgagcagg gctcaatact ccatcaaata ctttacctac
      121 gtgcaaacga ttatatttta gttcaggatg ctttagattt aacggggaac tggataaact
      181 gagccattcc cctattaacc tttccgtttc tggcattaaa gccatcccag cgagctcaga
      241 tgaaattaag ggaaatattt ctccaagcaa tacccatcca gaccagctgc cactctcttc
      301 tgctatcggt aaccatttag cactaaattc gggcattaat aatgcaacat cttcgccatt
      361 ttctttccaa ttttgaaaag tggtttcaaa cgtatgagtg tttcgattta ctttcctcag
      421 accgaacatt aactttcatc cttctgatcg ttttcgaatt tgctttcatt ttcttgctcc
      481 tcataaggct gatgtcgttg tccttgtcgt tcatcttgct cagtcaatac ccaacgttgt
      541 gctgtaactc attatgaaaa ctatttaatt ccctcatcat tgataaggtt ggacatctct
      601 ttcaacatga gagtcattga agctttcgac caatttagcg gaatgatagg gcttggcgct
      661 tcatgcaaag ttcccgactt gtggatatag ttatagcttt cgggaagtgc catttcaggt
      721 acactcctgc catttgcacc aatcatattc tcgcctgtta tttgggccaa tgatctattc
      781 ataaattgca ctgagtcatt caaatactct tcctttcttg attctttata aacaattgct
      841 gctgattttg cataccatga gtcaaaaaac cattctgctt ctgttgatgg aatgaagctc
      901 ttttctcttt tcgcatgtga attttgatcg gtatcggttt taatatcatt gaaccaaaaa
      961 tttgccgact gataattatc cttctcgtat ctttttatac catacggacc tgctagtttt
     1021 ttaactattt ttagaacctg ctcttttctt ctggtgttta tctttgcaag atttgcagga
     1081 taaataacat taagtaaagc cgcatcggct tctctgtaat gtttatcttt ttccagataa
     1141 cctggggatt ctccccctaa atcaagctgt aacgttattc tttcataacc tttatctatg
     1201 aggtggttca acctcgttgt tgaaagaggt tcatccagtt cattggcttt agcttcgcgt
     1261 agtaaatcag agacaaaaac agagtctttc ttcgagagta agttcgacaa acgttccagt
     1321 ccagaggtca ccagtgcaac ggaagatgtg tttaatcggg catcttcttc ccatgctccg
     1381 gaatcttcca taacatagaa attggcttta tccagataag caatcagcag cgcaaccgat
     1441 tttaatcggt cacctttttg ccaatcttca gcgttaattg tatcagtgtc gattgcttgt
     1501 ataaggagat caagataaag acctaacgca tcattttgtt tatggttcca cagctgcggc
     1561 ttgccttctt cctgcacatc tgccatcacg ggggagttgc tgtcaaatcg gatatgcaca
     1621 acattcattt gcccgggaac tccatccagt cgtttggggt tgctaatgac gtcctgcatc
     1681 cgcttaatct ggtcgggcgt agacatataa tcccacaggg tgagcagaac cttttttgcg
     1741 gctacactat ttccctgatc tgatactaat gccatatagc cccataaact atccctcagc
     1801 cagacagcat catagtgggt ttctgccgct gttttgtcat cttcgaaagt agaagcaaga
     1861 ataaacgttc cccagggttt ctccgtcaac gttaactttc gtgtgttttc tgttaatgtt
     1921 agcaatcctt gaatttcgtc tttggtatag tggctcttta tttcattttg taatttggct
     1981 gagtgaatca aaagacgata attaggatac ttatctggat ttaacgataa ataactttta
     2041 tctgttgctg cccccgtgga attaagaatg ttaaatacag attcactc
//
LOCUS       DAPPNJ010000338         2024 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000338 DAPPNJ010000000
VERSION     DAPPNJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.340"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            343..1682
                     /locus_tag="RPM73_004420"
                     /pseudo
     CDS             343..1682
                     /locus_tag="RPM73_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT          560 a          439 c          500 g          525 t
ORIGIN      
        1 ggcggtaaat acagcgaact ccattggttt agttggatat ttactgtgct ggacaaaaac
       61 ggtttgcggg gaaatcttag ttaagtagaa tgactgcggg tgcttgaggc tatctgcctc
      121 gggcatggac accaacggca gatagagaaa agccccagtt aacattacgc gtcctgcaag
      181 acgcttaaca ttaatctgag gccaatttca tgctagacac atgtaggtta gcctcttacg
      241 tgccgaaagg cacggagaag caggctattg ttaacaccaa gctgtaatgt cccctttgaa
      301 ccattctaaa atgtccccag acaattctct gggggatttt tcatgatcaa agagactgtt
      361 acgatgagtc ataaggaact ccaccgactt cagattattc aggagtcaat taatcgacat
      421 attactcagg aacaagctgc ggcacgcatt ggcatttcta ttcggcaggt taaacgtctg
      481 gtgcaacggt atagaaatga agggccttct ggtctggttt cccaccgacg tggaaagcgt
      541 cctaataatt ccttttctac tgaattcaga gcaacagtaa tttcactcct caaaggccgt
      601 tacgctgatt ttggacctac gtttgcgtgc gaaaaattgc gcgagataca cggtttatct
      661 ttatccgttg aaactctcag aaagtggatg atagaagaag ggttatggcg tgaacgccgt
      721 cgtaaaattg cccgtatata tcaacgccgc atgcgacgac catcttacgg tgaactgatc
      781 cagattgatg gctcacctca tgactggttt gaaaatcgag gccccagatg tacactgatc
      841 gttttcattg atgatgccac cagtgcgttg atgggttgcg ttttgtgcct gctgaaacaa
      901 cccgggctta catggaaacc ctccggggtt accttaatga tcatggcgta ccgctcgctc
      961 tctactctga tagacacagt atattcaggg taaataaccc agagcgggaa ggtgagctga
     1021 cccagttcac tcgtgcgata aagacactgg gcatcgagcc aatccatgcc aacagcccgc
     1081 aggcaaaagg gcgggtagag cgcgccaatc agacactaca ggacaggctg gtcaaagaaa
     1141 tgcggcttca gaatatcagt gatattgaaa cagcaaatgc atggttgccg acctttattg
     1201 aagcctataa caaccggttc gctacgtcgc ctcgtactac tgataatgct catcttgatg
     1261 tgcaccattc tgaagaggaa ctgggttata tcttcagcct acaggcgaag cgcgttctgt
     1321 ctaaaaatct cactttccag tacaaaagca gtgcgtttca ggtacgcagt gagagccggg
     1381 gatatcgact taggcattcg gttgttactg tatgcgagaa ctttgacggt gaaattaacg
     1441 ttctgtatga cgggaaagcg ctgggctggg aaaagtatgt tgatggcccg gagcctatac
     1501 cactggatga tgaaaagagt gtccatgaac gagtggataa tgcccgtatt gatttacgct
     1561 caaaatacta tgttaaacct aaagctgacc atccctggct tacgcgccga acgcaaagtc
     1621 atcagcaagt taagcccccg aagttaccta aaaagaagcc tgatcccgat aaaaaagatt
     1681 gaaaccaaga tcgattcggt tgagtgcata tccattcata gggtagattc ttaagtcgcg
     1741 tttctggtgt tcattttcgg gtggtttgtt acttgtttta ccggggatat gccagaaacg
     1801 cgctgagtca gtctgggcgg tgcgcgtaat gaggcgttat ggtaaatagc ctatgctaat
     1861 gtccgctaag agcaagaagc ggaagttggc agttttgtgg actgtcccca caaaagtgac
     1921 tacagaaata gttgcaattc ataattgatc atgggttgtc agttaaactc gtggcgattt
     1981 aaatagacta attgggagtg cgtccattac ttatatcttg taat
//
LOCUS       DAPPNJ010000339         2001 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000339 DAPPNJ010000000
VERSION     DAPPNJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.341"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            86..1306
                     /locus_tag="RPM73_004421"
     CDS             86..1306
                     /locus_tag="RPM73_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX6522674.1"
                     /translation="MGKALNKLSDSTLKKLAAIQAEKERFYSDGGGLEIKHSKGGKLT
                     WYFRYRTGGREVAAERLKLGAYPELSLKAAREKRTLCRAWLAEGKNPRYELCATVQEA
                     LKPVTVKEAINYWLEEYAKDNRKDYIKLVQRMDKHIICHIGAIPLDKCDTRQWIACFD
                     RVRKKAPVAAGHVMQTCKQALKFCRRRRYAFSNALDDLIVTDVGKRAEIRERVHSNSE
                     LKEILRAIDGDVFAPYYSALMRLLIVFGCRTAEIRLSEIKEWDLKEMLWTVPKEHSKT
                     KVTIFRPIPDGILPFIQKLVEQNAHTGLLLGELKKDTTVAQYGRNAHKRLKQEHWTLH
                     DFRHTFTTMLNDLGVDPHIVEHITAHQMPGQQKTYNHSRYLQAKRDALNLWVERLDMI
                     AGYNENIVILRGIQ"
     gene            1303..>2001
                     /locus_tag="RPM73_004422"
     CDS             1303..>2001
                     /locus_tag="RPM73_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522675.1"
                     /translation="MTKKVNSKKDLPKSFDLGKYDCLENLSDKDLFRQLYWRQDDLTM
                     KHSEMPEYGFMFGAEYPLHNNYGDPFGELKEDDWFCDKQKEYDHKVQPKLIELSYDDG
                     IKPVTRFDISMINKLTAERGYWKDKPIIIDNEMVGSLISEDNGMFWAVMREPVNLLSD
                     TLDNMLVSVDLLHNRDDELIEAFTKLLPKWRSELSIVEPDKPIAGSWESIRRKIIDYK
                     IIPLIDLLSWELSTD"
BASE COUNT          641 a          350 c          512 g          498 t
ORIGIN      
        1 cgttgttcgt agagcttcaa caaaatgtgt ggtcagttgt gtggtcagtt ttttgaggct
       61 ggaggactga aaagaggatc tgatagtggg aaaagcacta aacaaactga gcgattcgac
      121 gttaaaaaaa ttggcggcta tccaggcaga aaaagagcgt ttttactccg acgggggcgg
      181 gctggagatt aaacactcaa agggcggcaa attaacctgg tatttccggt accgaacggg
      241 aggccgtgag gttgccgcag agcggttaaa gctgggggct taccctgaat tgtcgctgaa
      301 agccgcaagg gaaaaacgca cactgtgccg ggcatggctg gctgagggta aaaacccccg
      361 ttatgagttg tgcgccacag tacaggaagc actaaaaccc gttacggtga aggaagcgat
      421 taactactgg ctggaggaat acgcgaagga taaccgcaaa gattacataa agctggtgca
      481 gcgtatggat aagcacatca tttgccatat tggggcaatt ccccttgata agtgtgatac
      541 aaggcagtgg atcgcatgtt ttgaccgcgt acgaaaaaaa gcaccagtag cagcgggcca
      601 tgtcatgcag acatgcaaac aggcgctaaa gttttgccgc aggcggcgct acgcgtttag
      661 caacgccctg gacgatttga tcgttactga tgtgggtaag agagcagaaa tccgcgagag
      721 agtgcacagc aacagcgaac taaaagaaat tctacgcgct attgatggtg atgtgttcgc
      781 tccctattac agtgcgttaa tgcgcttgtt aattgtgttc gggtgcagaa cggcagagat
      841 cagactttca gagatcaaag aatgggatct gaaagaaatg ttgtggacag tgccaaaaga
      901 gcacagcaaa acgaaggtaa caatattccg acctattcct gatggtattt tgccgttcat
      961 tcagaagctg gtggagcaaa acgcacacac tgggttatta ctcggcgaac tgaaaaaaga
     1021 taccacggtg gcgcaatatg gacgaaatgc gcataagcgg cttaagcagg aacactggac
     1081 gctgcatgat ttccgacaca cgtttacaac tatgctgaat gatttaggtg tcgatccgca
     1141 tatcgtggag cacatcacag cgcatcagat gccaggtcag caaaaaacct ataaccattc
     1201 acgctatttg caggcgaaac gggacgcact gaatctatgg gttgagcgtc ttgatatgat
     1261 tgcaggatat aatgaaaata ttgtgatatt gagagggata caatgacgaa aaaagtaaat
     1321 agtaaaaaag atttaccaaa gtcatttgat ttaggcaaat atgattgcct agaaaacttg
     1381 tcggataaag atctatttcg ccaattatat tggcgacaag atgatttaac gatgaaacat
     1441 tctgagatgc ctgaatatgg atttatgttt ggtgctgaat acccgttgca taataattac
     1501 ggggatccat ttggagagct gaaagaagac gattggtttt gtgataaaca aaaagaatat
     1561 gaccataaag tgcaaccgaa gttaattgag ctaagttatg atgatggaat taaacctgta
     1621 acaaggtttg atatatcaat gattaataaa ctgactgcgg aaagaggata ttggaaggat
     1681 aaaccaatca tcatagataa tgagatggtt ggaagtttaa tatcagaaga taatgggatg
     1741 ttttgggctg tgatgcgtga accagtgaat ctcctttctg atacattaga taatatgctt
     1801 gtttctgttg acttactaca taacagagat gatgagttga ttgaagcgtt tacaaaacta
     1861 ttaccaaaat ggcgtagtga actttcaatt gttgagccag acaaaccaat tgcaggaagt
     1921 tgggagagca tacgacgtaa aattattgat tataagataa tcccattaat tgacttatta
     1981 tcttgggagt tgtctacaga t
//
LOCUS       DAPPNJ010000340         1986 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000340 DAPPNJ010000000
VERSION     DAPPNJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.342"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>1986
                     /locus_tag="RPM73_004423"
     CDS             <1..>1986
                     /locus_tag="RPM73_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDX6522676.1"
                     /translation="RFNIINDKENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQL
                     FNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELAT
                     IDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETY
                     QLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYAN
                     KKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKL
                     QITAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSG
                     MNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFA
                     QQMYETRYADDRTKLLQENQIVQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVA
                     LITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQFSQWKRYDSRTPVNLA
                     TRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDE
                     LLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPA
                     AWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLL"
BASE COUNT          585 a          452 c          468 g          481 t
ORIGIN      
        1 tgcgctttaa tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc
       61 gcctgccaat ggtacaagtg aacgatgagc aaagctttat cgaacaagct gaatggagca
      121 tgttagttca gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga
      181 ctatttctgg cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt
      241 tccgtgtaaa tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt
      301 tggcaaccat tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc
      361 gcctcacctg gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga
      421 ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat
      481 atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc
      541 agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg
      601 ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa
      661 agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac
      721 aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca
      781 atggtgccag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattactg
      841 ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg
      901 caaataaagc agtaagcgga agcggcgttg gtgagctctc ctcttccagc ctgaaacgct
      961 ggagtgcgga aaattcggta accatgagca gtaaagtcag tggcatgaat acgttgctct
     1021 ccgttagcgc gcggactaat aaccctgaac ctggttttca gttgattaac cagcgaatca
     1081 cccacagcac gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt
     1141 tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgtat gagacgcgct
     1201 atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgtacagttt actgccgcag
     1261 atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg
     1321 tcggtaatgt ctcagaagac aaactcgtgg cgttaattac gcgttactta ggatcaatca
     1381 aacactctga ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat
     1441 cggttactgt aaaagaacaa aatgaacctg tggcacagtt ttcacagtgg aagcgttatg
     1501 attcccggac acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac
     1561 tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc tggagcatac agcgtttctt
     1621 ctcgcctctc ggttgatcct caggccaaag atatcagtca tttgctggct tttacttgtc
     1681 aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt aagcgtctgg
     1741 ctaaagggat cagtgagcaa gaactgaatg aataccagca aaacgttcag cgcagcctcg
     1801 atatccaaca gcgtagcgtt caacaattag cgaacactat tgtaaatagt cttattcaat
     1861 atgacgatcc tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga
     1921 atgttaacac tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat
     1981 tattgc
//
LOCUS       DAPPNJ010000341         1959 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000341 DAPPNJ010000000
VERSION     DAPPNJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.343"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            307..1692
                     /locus_tag="RPM73_004424"
     CDS             307..1692
                     /locus_tag="RPM73_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522677.1"
                     /translation="MDSVSSVERLRTKAFYQLLVDRLGTEAWAARKAAYFKRIREKEA
                     KFNINLPIEPQLFIPAEDDIDWYILASYLSHDFPYSDATYSSRRIYPYAMAIGAVADQ
                     LRKVPYVDDVLDKMLANNNKPETQIFELLTASFYLKNGYEVAFIPESSIVWPDGKTKK
                     SPDMLVKLGDLEFYVECKRSDKQTKYSKTEEQAWANIWHQLSQHMLKVAPWNIVDIIF
                     HEQVASVTPADVIKAVNLAIKGGTGKANEGSISVGIRAIDKLGLRRHYRKFSVRANSP
                     QHELLVFGELDSNEKRSISTIAQRVIRPGTNNDILNIFVEDVAKCVGAQWRCDHEISL
                     GLRSKHFKSLLNDGVMQIPPDRAGVVHIWYETCEGIDIEELRRDKNMEHISAYDASKT
                     TVLGAFLHAVNYYPLEDNYEWAETVQDFARVPDLMGLFLRQPLMLTSDSTRGVEGVTH
                     WEQDKAAKSMR"
BASE COUNT          559 a          420 c          467 g          513 t
ORIGIN      
        1 tggcggcctc agtccggaac aatttgaaaa caagaacctc gcttaggcct gtgtccatat
       61 tacgtgggta ggatcatagg gcttaaccta agctgacagt ccgctctgtg ccagaagcgg
      121 acgttgcggg acgtcagata tctaaaaaaa ctaatggtcc gcttggatag atacttacac
      181 aggatgcaat aaagagtttc tatttctttg tggtagcaaa ctttgatttc tgaaggataa
      241 tacctccaat aagttgaaat cacatatatc gctaaagact ctatacaaca accaagagag
      301 accgcaatgg atagtgtcag tagtgttgag cgtttaagaa caaaagcttt ttatcagttg
      361 cttgtggata ggcttggaac tgaggcctgg gctgctcgca aagctgcata tttcaagcgc
      421 ataagagaga aggaggctaa gtttaacatc aatctgccga tagagcccca gctcttcata
      481 ccagcagaag atgacatcga ctggtacatt ctggcgtcgt atctgtctca tgattttcca
      541 tatagtgatg ctacttacag cagcagacgt atctacccct atgctatggc catcggagct
      601 gttgcagacc agcttcgcaa agtcccatac gtcgatgatg ttctggacaa aatgctagca
      661 aataacaata aaccagaaac tcaaattttt gagctgctga cggcatcgtt ctacctaaag
      721 aacggctatg aggttgcctt catcccagaa agcagcattg tttggcccga tggaaagacc
      781 aaaaaatcac cggatatgct tgtcaagtta ggtgatctgg agttctacgt cgagtgcaaa
      841 aggtcggata agcaaaccaa gtactcaaag acagaagagc aagcctgggc caacatctgg
      901 catcaattga gccaacatat gctcaaagtc gcaccatgga acattgtcga tataattttc
      961 catgagcagg tcgccagtgt gactccggca gacgttatta aagcggtgaa tctagccatt
     1021 aaagggggga cgggaaaagc taatgaaggt tcaatcagtg tgggaatacg agccatcgac
     1081 aaattaggtc tcagacgtca ctacagaaaa ttttcagtca gagccaatag cccacagcat
     1141 gaattgctgg tatttgggga gctagatagc aatgagaaac gaagtatctc gactatcgct
     1201 cagagagtta ttcgtcctgg caccaataac gatattctca acatttttgt agaggatgtt
     1261 gcaaagtgcg ttggagctca gtggcgctgc gatcatgaga tttctcttgg cctcagatcc
     1321 aagcatttca agagcttgct caatgatgga gtcatgcaga ttccccctga tcgtgctggc
     1381 gtggtgcata tttggtatga gacttgcgaa ggcatagata ttgaagaact acgtagggat
     1441 aaaaacatgg agcatatctc ggcctacgat gcctctaaaa caactgtgtt gggtgctttc
     1501 ctgcatgccg ttaattatta cccattagag gacaactatg agtgggctga gactgttcaa
     1561 gactttgccc gtgtcccgga tttgatgggg ctcttcctta gacagccatt gatgcttaca
     1621 tctgactcga cgcgtggggt cgaaggtgtc acacattggg agcaagataa ggccgcaaaa
     1681 agcatgcgtt gaaagtggct ggaatatggc gatactggga acataactat tcaccgcgtt
     1741 tcataaccca ctctcggctg attaacacac gttatcgttg ggggaaagga ccaattccct
     1801 acgtccgctc ctcgctcaaa gcagactgtc agatttggtt gcattttgcc tacgcaaact
     1861 gtcagttcga gcctgagcta atacagctaa ctggatgtta gcctttttta attttatttt
     1921 tagattgttc aatgtgatga tttaacactt ctctctttg
//
LOCUS       DAPPNJ010000342         1902 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000342 DAPPNJ010000000
VERSION     DAPPNJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.344"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="RPM73_004425"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="RPM73_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDX6522678.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="RPM73_004426"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="RPM73_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDX6522679.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="RPM73_004427"
     CDS             902..1549
                     /locus_tag="RPM73_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDX6522680.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLLNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..>1902)
                     /locus_tag="RPM73_004428"
     CDS             complement(1591..>1902)
                     /locus_tag="RPM73_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6522681.1"
                     /translation="PFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEAAVSLS
                     RKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYL
                     V"
BASE COUNT          550 a          379 c          442 g          531 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtaagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attactgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggc actttcccca ggcagcgttt aatattaaag gg
//
LOCUS       DAPPNJ010000343         1897 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000343 DAPPNJ010000000
VERSION     DAPPNJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.345"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1058
                     /locus_tag="RPM73_004429"
     CDS             <1..1058
                     /locus_tag="RPM73_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDX6522682.1"
                     /translation="HIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYR
                     RQGVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRT
                     ARLWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYE
                     SGRITEAACMAHARRKIHDVHARASTYITTEALQRIGELYAIEAEVRGCSAEQRLAAR
                     KARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIA
                     ENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWP
                     ANRVRDLLPWKVDLSSQ"
     gene            complement(1140..>1897)
                     /locus_tag="RPM73_004430"
     CDS             complement(1140..>1897)
                     /locus_tag="RPM73_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDX6522683.1"
                     /translation="SGLMAVILTGCGNLSNVSPEGTTDEPVWPSPDRTMFSHSGTQKG
                     SWPVPEKLRLIKAGMNKDQIYQLAGRPHFGEGIAGVREWDYLFNFTGPDGDYVCQFKI
                     LFDRHMDARSFFWKPENCSQEQEVKRYERTLQGDILFDHDSSVLTEKGREQLMIVAEE
                     LRKEGGRRFIIEGHTDHTGSEPYNNALSEKRAGTVKGWFISQGFDAGNIEIRGKGDKE
                     PLVVCENTGDEVMKECLRPNRRVVITAEEEGDR"
BASE COUNT          452 a          524 c          467 g          454 t
ORIGIN      
        1 accatatcgt gcaggcacca gtaccttcaa aacccattgc acgcagttat gccggagcgg
       61 ggcttctggc ccatgttgtc accgggaaat atgcagacca tctgccgtta taccgccagt
      121 cagaaatata ccgtcgtcag ggagtggagc tgagccgtgc cacactgggg cgctggacag
      181 gtgctgttgc tgaactgctg gagccgctgt atgacgtcct gcgccagtat gtgctgatgc
      241 ccggtaaagt ccatgctgat gatatccccg tcccggtcca ggagccgggc agcggtaaaa
      301 cccggacagc ccggctgtgg gtctacgtcc gtgatgaccg taacgccggt tcacagatgc
      361 ccccggcggt ctggttcgcg tacagtccgg accggaaagg tatccatcca caaaatcacc
      421 tggccggtta cagcggtgtg cttcaggccg atgcttacgg tggttaccgg gcgttatacg
      481 aatccggcag aataacggaa gccgcgtgta tggctcatgc ccggagaaaa atccacgatg
      541 tgcatgcaag agcgtccacc tacatcacca cggaagccct gcagcgtatc ggtgaactgt
      601 atgccatcga ggcagaggtc cggggctgtt cagcagaaca gcgtctggcg gcaagaaaag
      661 ccagagccgc gccactgatg cagtcactgt atgactggat acagcaacag atgaaaacac
      721 tgtcgcgtca ctcagatacg gcaaaagcgt tcgcatacct gctgaaacag tgggatgcac
      781 tgaacgtgta ctgcagtaat ggctgggtgg aaatcgacaa caacatcgca gagaacgcct
      841 tacggggagt ggccgtaggc cggaaaaact ggatgttcgc gggttccgac agcggtggtg
      901 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggagccag
      961 aaaagtggct gcgttacgtc attgaacata tccaggactg gccggcaaac cgggtacgcg
     1021 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     1081 tcgcttaccc ccgaaaaata aaagcatata aataacaagc tcaggtatat cagatctatt
     1141 tacctgtctc cttcctcctc agcagtaatc accactcttc ggtttggacg aagacactcc
     1201 ttcataacct catcccccgt attttcacag acaaccagag gttccttgtc acctttaccc
     1261 cggatctcta tatttcctgc atcaaaaccc tggcttataa accacccttt gactgtgccg
     1321 gcccgtttct ctgacagtgc gttattatat ggttctgacc ccgtatgatc tgtatgacct
     1381 tctataatga aacgcctgcc cccttccttc ctgagttctt ctgcaacgat catcagttgt
     1441 tcacgtcctt tttctgtcag cactgatgaa tcatgatcga acagaatatc cccctgtaat
     1501 gttctctcat atctttttac ttcctgttcc tgactgcagt tttccggttt ccagaaaaat
     1561 gatcttgcat ccatgtgcct gtcgaaaagg atcttgaatt ggcagacata atctccgtca
     1621 ggcccggtga aattaaagag gtaatcccat tccctcactc ccgctatccc ttcaccaaaa
     1681 tgaggacggc ctgccaactg atatatctga tccttattca ttccggcttt tatcagacgc
     1741 agcttctccg gtacaggcca ggagcctttc tgggttccgc tgtgactgaa cattgtcctg
     1801 tcaggagaag gccacacagg ttcgtcagtt gttccttccg gtgaaacatt actgaggtta
     1861 ccgcacccgg tcagaataac cgccatcaga cctgata
//
LOCUS       DAPPNJ010000344         1849 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000344 DAPPNJ010000000
VERSION     DAPPNJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.346"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            100..354
                     /gene="bssS"
                     /locus_tag="RPM73_004431"
     CDS             100..354
                     /gene="bssS"
                     /locus_tag="RPM73_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDX6522684.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            469..1587
                     /gene="solA"
                     /locus_tag="RPM73_004432"
     CDS             469..1587
                     /gene="solA"
                     /locus_tag="RPM73_004432"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDX6522685.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1635..1748
                     /locus_tag="RPM73_004433"
     CDS             1635..1748
                     /locus_tag="RPM73_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDX6522686.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          475 a          447 c          444 g          483 t
ORIGIN      
        1 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
       61 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
      121 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
      181 atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt
      241 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
      301 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
      361 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
      421 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
      481 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
      541 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac
      601 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc
      661 ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt
      721 gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc
      781 cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
      841 cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
      901 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
      961 gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt
     1021 gaaaccgttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta
     1081 aaagacctgc tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag
     1141 gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
     1201 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
     1261 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat
     1321 ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac
     1381 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc
     1441 ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca
     1501 gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg
     1561 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
     1621 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
     1681 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
     1741 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttggcg
     1801 tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacat
//
LOCUS       DAPPNJ010000345         1846 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000345 DAPPNJ010000000
VERSION     DAPPNJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.347"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1475)
                     /locus_tag="RPM73_004434"
     CDS             complement(<1..1475)
                     /locus_tag="RPM73_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522687.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSIDWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQASQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPQSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGW"
BASE COUNT          547 a          413 c          356 g          530 t
ORIGIN      
        1 ttccaaccga tcttcggcca ggctcgatgg acaggataat ccgatttaac cagttcaact
       61 tctgtcgatt taatataata ttcaatcgca gaatcataaa gttgctgggt gcgaattgta
      121 caaactgcac catcatgtaa aacaacgcta aatactccac ttattttagt taagctgcgt
      181 tcactgtttt tcagcaacct tgggatatca aactcacctt ctccactaat atctaaatgg
      241 ctatttttcg gtagcgaaat aaacagagca tttagcctgg aactcacaga ccccatacca
      301 acaagtttta gctgcgattc actctcattc atcgcttcaa aaacccaggg aagttcttca
      361 gtaagttcat aaccgccttt tggaattgtg tgacctaaca gaattggacc ttcatgaagc
      421 gacaatgaga tttcggccat tgcatctgca ccagagagtt tttgcatcgc aataggtaat
      481 aactcgaccc gccaatcttg ctgctcataa cggcttagca ttgccaacct tgccccgcca
      541 tttttccatt ttcctgaaat gatgagtcgg gtctgctccg gctgaatatg gcattcaaac
      601 aaagaggtta actgttctgt acgcattgtg gccgggaatc ggaaccgggc atcacaatac
      661 caactgtcat caacatcgac ccagattctt tcaacctgaa gactacttgc actgcgtatt
      721 tcagaagatt gagaaagtaa tcgccttaca atctctgcag cattcgcttc ggggagaacg
      781 agtggaagtt gaagatgcca gtctggagag agcttacgta atgcagaaac gggatcactg
      841 ctttgaggtg gatgctcact aagtaatcgg cagagagtct cgcagaattc accggctatt
      901 tcatagacca gttcgttttg cattgtgaca ggaatcgtat cgcctaattc ttgtgcaatt
      961 ttagcggcag gatattgaga tgcttgccca cgaagcgcct gatatatcct tctgaaatag
     1021 gtaatcaaat aaccgctctc attttcaatc atgcggatag gtaaaccacc ttcacaagcc
     1081 agtgtatgca gataacccga agcttgcccc aggtttctta tctttctttt ccagtagcga
     1141 atgccatcat taaccagctt ctgtcgatga acatacggaa ctttccagtc aatagaatct
     1201 aaaattgtgt cccatttagg gtgtccgacc gtatgagtcc ggcggataaa ctctgcggca
     1261 tatatgctaa atagaacatc gctataggaa atgaatatcg ttcttggata actacgaggg
     1321 gcatatgtac gcagtagttc tgccaaactt tcgtactctg tatcagtaca acggtaagca
     1381 tataacgccc gtccatcagc tccggataat gaacggcgac taaaaaacat gttaagccat
     1441 tgcaaaagtg ggttaactgg agttttcaca agcaacctgc cttcctttga tacaattcgt
     1501 aacaggttac tatcatcata aaaaagctca acccgatgaa ctcgctaaaa atgagacaaa
     1561 tcatttatat ctcgaaaaaa cttgttacaa tcatgagcgc tacaccgaac ttaaccatat
     1621 aaattatgtg tgttttgttt attttttaaa cgattacaac tatccattat ttacacaggt
     1681 atcaaaatgt tagcgcagct ttttgagcag ttgtttcaat cgatagactc tacactgatc
     1741 accaatattt tcatctgggc tgttatattc gtatttttat cagcgtggtg gtgtgacaaa
     1801 aaaaatatac atagtaagtt tagagaatat gctccaacct taatgg
//
LOCUS       DAPPNJ010000346         1824 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000346 DAPPNJ010000000
VERSION     DAPPNJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.348"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(62..823)
                     /gene="kduD"
                     /locus_tag="RPM73_004435"
     CDS             complement(62..823)
                     /gene="kduD"
                     /locus_tag="RPM73_004435"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDX6522688.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(853..1689)
                     /gene="kduI"
                     /locus_tag="RPM73_004436"
     CDS             complement(853..1689)
                     /gene="kduI"
                     /locus_tag="RPM73_004436"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDX6522689.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT          482 a          485 c          405 g          452 t
ORIGIN      
        1 atgatctaca ggtataaaaa accctgccat gcggcagggt cataaaagta agaagaatga
       61 attaacgcgc cagccaaccg ccatccacgg caatggtata accattcaca taatctgaag
      121 cgctggaggc aaggaacact accggcccca tcaggtcact cggcagtccc caacgaccag
      181 ctggaatgcg gtcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgagtat
      241 tgttggtcgc catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt
      301 cgttcgccat caatcgcgtc acgcccatca caccgctttt tgatgcggta taagaaggca
      361 cacggatccc accctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc
      421 cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca
      481 ggttcatgac atcgtcccag tccttttcgc tgaactcgag agcgtcttcg cggcgaatca
      541 atccggcgtt attcaccagg atatcaatat gaccaaactc cgctaccgcg cgatccagca
      601 gtgctggaat accatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca
      661 gcgctgtgac ctgcttgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac
      721 agcccgcttg cgccagcccc aacgccatcc cctgacccag tccagtatca caaccagtga
      781 cgaccgcaac tttaccttcg agagaaaatg cacttaaaat cataacaata ccttattcgt
      841 ttatgcccac aactagcgca aatctttaac ggccacatgg tccatatcat caaagacctg
      901 gttttcaccg accatgcccc agataaaggt ataagctttg gttccgacac cggaatggat
      961 cgaccagctc ggggagatca ccgcctgctc gttatgcatc acaatatgac gcgtttcttg
     1021 cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac
     1081 ttccatccgg cgctcgtggg tgtgacacgg catggtgttc cacaagttac ccggagccag
     1141 ctccgtcagc cccatactca attggcaggt ttccagtaca tccgggacaa aatatttgtt
     1201 aatcgtgcga cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg
     1261 tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc
     1321 cggagtgccg gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag
     1381 ggcgtcgcgg tgaccgattt catagcattg gccatcgaca gtaatcgtac cggcaccgcc
     1441 aatattgata acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc
     1501 aacttccccg ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg
     1561 gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa
     1621 ttcattgcgc agcccttggg tatccagcgt ttttgcgtgc gcactgtgga tgctctgtct
     1681 tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac
     1741 actgtttcgt tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa
     1801 ataaaacaat gtttcatttt taag
//
LOCUS       DAPPNJ010000347         1817 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000347 DAPPNJ010000000
VERSION     DAPPNJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.349"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            457..804
                     /locus_tag="RPM73_004437"
     CDS             457..804
                     /locus_tag="RPM73_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522690.1"
                     /translation="MVVLVVAPEEQVHSEIQLTEKRYQQLGLEVAVLDYVLIHQRVSR
                     VPEQMEQFIFTGEVHMWARIEENKVEEITEINPEGRFHPSLIWKECPADTQQGDLYID
                     GEFIAQSPAVQAR"
     gene            complement(770..1378)
                     /locus_tag="RPM73_004438"
     CDS             complement(770..1378)
                     /locus_tag="RPM73_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDX6522691.1"
                     /translation="MDNAVLNSEFIATKAGNITVYNYDGETREYISTSNEYLAIGVGI
                     PAYSCLDAPGTYKAGYTICRSVDLKSWEYVSDHRGEIVYNTETGDAKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RNLTQSETSRLNTVLDYIDAVTATDTSTAPDVIWPELPETAQ"
     gene            complement(1378..1803)
                     /locus_tag="RPM73_004439"
     CDS             complement(1378..1803)
                     /locus_tag="RPM73_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDX6522692.1"
                     /translation="MPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRG
                     EFIRGWDDGRSVDTGRSVMSAQSARVGYIKFDMGIYGIPAGTGSLILSNDILITGHDS
                     NGSSLLLNNSNERAIGEMYVVPADTRPRNIAFNYIVRAA"
BASE COUNT          463 a          395 c          482 g          477 t
ORIGIN      
        1 gagaaggttc atttgggttc agaaatctgg ttgccttcac aacggctgga gtaacaagct
       61 ggactgttcc tgaagagttg cgcaaaggaa gaaaatgtta cgtcaaagtt attggtggcg
      121 gtgcgtcagg agggataggt tcaacaaccg cttcagccgt tacatgtggc ggaggaggtg
      181 gtgggggcgg agttgctgag gggctggtag atttaactgg tataaacagt gtttcgatta
      241 cggttggtgc cggtggtgca cctgtgtctg gtatttctgt aaacggtaaa aatggtggct
      301 cgagcagttt cgggacttac atgtcagctt ccggaggcta tagcggaggc cagccttccg
      361 gagggttagg cgcagttggt gtcgggggaa caattaatac atcgcttggg ccaggttctc
      421 caggttccat tgcttctgcc gcatcgggag ctggtaatgg tggttctggt ggtggccccg
      481 gaggagcagg ttcattcaga gatacagcta acggaaaagc ggtatcagca gttgggcctg
      541 gaggtggcgg tgctggatta tgtcctaatt catcaacggg tcagtcgggt gccggagcag
      601 atggagcagt ttatatttac tggtgaggtt catatgtggg caagaattga agaaaataaa
      661 gtagaagaga taacggaaat taatcctgaa ggaagattcc atccatcatt aatatggaaa
      721 gaatgccctg ctgacacaca gcagggggat ttatatattg atggtgagtt tattgcgcag
      781 tctccggcag ttcaggccag ataacatccg gcgcggtgct ggtatctgtt gccgtcaccg
      841 cgtcaatgta atccaacacc gtgttaagtc ggctggtttc tgactgcgtc aaattccgtc
      901 cggcctgtaa tttcagctga atcagactaa tggaagccat tgcagtatca atcagtgact
      961 gacgctgtgc ttctgccgcg tctactgcag cgctatgctg tgcctcagta tcggtcaccc
     1021 atttctcacc atcccattta tcgtatggcg ttaatggggc gatagtggtt gtattttctg
     1081 gataatcacc cggagctgtg atttctttgg cgtctcccgt ttcggtgtta tagacgattt
     1141 caccgcgatg gtctgacaca tattcccatg attttaaatc cacagaacgg caaattgtat
     1201 aaccagcctt atatgtacca ggagcgtcta aacaggaata tgcagggata ccgacaccaa
     1261 tggcaagata ttcatttgaa gtggaaatat attcccgcgt ttcgccatca tagttataga
     1321 cggtaatatt tcccgccttt gtagcaataa actcgctatt taatacagcg ttatccatta
     1381 tgcagccctc acgatatagt taaatgcgat attccgtggg cgagtatcgg caggaacgac
     1441 atacatttca ccaattgccc gctcattaga gttgttcagc aataggcttg agccattact
     1501 gtcatgaccc gtaattaaga tatcattgga caaaataagt gagcccgtac ctgcaggaat
     1561 accataaatt cccatatcaa atttaatata accaacacgc gcgctctgtg cagacataac
     1621 gctgcgacca gtatctactg aacgaccgtc atcccagcca cgaataaact caccgcgtaa
     1681 atcaggcaat ttatttgtcg ggtaagcctt tgccagttcc gggtattctt cagcagaaaa
     1741 agccgcacca ttgcatttca gccagcctgt tggcggagtg gctgaaggcc acggaacagg
     1801 cacaccaacg ggtaatg
//
LOCUS       DAPPNJ010000348         1812 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000348 DAPPNJ010000000
VERSION     DAPPNJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.350"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            97..729
                     /locus_tag="RPM73_004440"
     CDS             97..729
                     /locus_tag="RPM73_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX6522693.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1063..>1812
                     /locus_tag="RPM73_004441"
     CDS             1063..>1812
                     /locus_tag="RPM73_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6522694.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNY"
BASE COUNT          605 a          329 c          333 g          545 t
ORIGIN      
        1 caatttttcc cagcaaaatt acaaaatcat ggtgtattga catcatcaat caccaatgaa
       61 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
      121 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
      181 ttgcagtact gcgcaaactc gtgtttttgt atgtggcccg aagaaagttc atattttaat
      241 cgtggtgtgg tagaaggtat cttaacaaaa aaccacaatg cgagattaag cggatatatt
      301 tttgtcgatt tttcagtaag ttttttacgt ctattccttg aaaaagactg gattgactat
      361 cttgcaagta ctgacatggg gattgtttta gtcagcgacc gcaatatgca atcgttagct
      421 aattactggc gaaaacacaa ctctgcaata tctgctgtca tatataatga tgatggtctt
      481 gatgttgcca atgaaaagat cagacaacta tttattggtc gttatttatc atttaccaga
      541 ggtaacacat taactcagat ggaattcacc ataatggggt atatggtttc tggttataat
      601 ccatatcaga ttgctgaagt tctggatatg gatatccgta gcatctacgc gtacaagcaa
      661 cgaatcgaaa agagaatggg tggtaaaata aacgaattat ttattcgttc acattcggtc
      721 caacattgat actcaacaat ctgccagaat catactctgt ttatacgggc atttattgaa
      781 atgcccgtat tttataaata ggccgtcccc tcaagagtaa acaccatagt atttaaaacc
      841 atcacacgaa aaattcagaa gcattacgaa atatgaactt acaaacaata gtggcataaa
      901 tgttaaccat gttaatttac ataaagtttt acgttgcgac attaaagcct catttcaatc
      961 atcatgataa atataaaatt aatatatatt tatgccgtaa ataccaatat acttagcaaa
     1021 ctatgtgatt tccatttcga ttgatttagt gtttattgac gtatgtactg gattattaac
     1081 gataatatcg agttctggcc tgagcaccga aaattaatat cggtacataa cgccgatctt
     1141 aacgtcgttc tgacaacgcc agccagtcgt tgtttatcac tgctacttga agcttttcct
     1201 gatgtggttg cacaacaaga ttttttcacc agagtctggg aagaagaagg tatgcgtgtg
     1261 cctactaaca cgttatatca gaacatatcc attatcagac gcggatttcg cgctgttggt
     1321 gatactaccc actcgcttat tgcaaccgtg ccgagaagag gattcaagat ccataatgac
     1381 attaacatac aaaatcatgt aataaactca tcaacagacg cacatacaca caatgcccca
     1441 cctgccataa aagttaatgc gggatacaaa gagagcattg gtggcgcaaa gaatttcaat
     1501 aacaaaatcc tcaaacatat aaaaagttat ctaattatgt tgagcgcatt tgtcataggc
     1561 gcatattctg catattggct atggaataat aatcaaccga aaccattttt caaggattac
     1621 aagactgttg cggaaattaa tggctgccat tttaatgtaa cagaagatac aattgatggg
     1681 ttgaaagagt tcgataaata taaaacacgg atactggatt cggggattaa ctgtaaaaaa
     1741 tatccgtggt tatacttccc tcttgcaaaa tcctcaccgg ggatgattgt tatggcatgc
     1801 aataaaaact at
//
LOCUS       DAPPNJ010000349         1806 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000349 DAPPNJ010000000
VERSION     DAPPNJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.351"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>1806
                     /gene="rhsB"
                     /locus_tag="RPM73_004442"
     CDS             <1..>1806
                     /gene="rhsB"
                     /locus_tag="RPM73_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDX6522695.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     MRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSF
                     TDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAE
                     RWRYDERGWLTDISHISEGHRVAVYYGYDEKGRLTGERQTVHHPQTEALLWQHETRHA
                     YNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYE
                     LTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHT
                     TAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWP"
BASE COUNT          436 a          555 c          542 g          273 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg gccggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgatgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagagta
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggccag acgggctgga
      481 aatgcgccgc aaatatgatg agtacggacg tctgattcag gaaaccgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgacctc ccctgcgcaa cggacgatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacggt cagttgctga gcttcactga
      661 ctgttccggt tatgtaaccc gctatgacca tgaccgtttt ggtcaggtga cggcggtgca
      721 ccgcgaggaa gggctgagtc agtaccgcgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaagccat
      901 ctgtaccacg cagggcggtc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg aagcagaccg
     1141 cctcacgcac cgcaccgtga atggcgaaac cgcagagcgg tggcggtatg acgaacgcgg
     1201 ctggctgaca gacatcagcc atatcagcga agggcaccgg gtggcggtgt attacgggta
     1261 tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc
     1321 actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg
     1381 tataccggac agcctgcccg ccgtggaatg gctgacctac ggcagcggtt acctggcagg
     1441 catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac
     1501 gctgcgccgc ttcggtcgtt atgaactcac caccgcttac acccctgccg ggcagttaca
     1561 gagccagcac ctgaacagcc tgctgtctga ccgcgattac acctggaacg acaacggcga
     1621 actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagca ccaccggcag
     1681 gctgaccggc gttcacacca ccgcagcgaa ccttgatatc cgcatcccgt atgccacaga
     1741 cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg
     1801 gccgga
//
LOCUS       DAPPNJ010000350         1782 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000350 DAPPNJ010000000
VERSION     DAPPNJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.352"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            325..846
                     /locus_tag="RPM73_004443"
     CDS             325..846
                     /locus_tag="RPM73_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDX6522696.1"
                     /translation="MSHDAEKKIRLCIQCENEALKDSDFCAVCEEREFKKIRGWLYLP
                     AIGLVLTILANMISINFTIRFLMENIAQIGHAQKGILFFELFAFIGMFSYGIFVSSLF
                     FRKKRQLPHHYIVLVGIGTIFVAVDLLLGHIYLDVPYVFDTVKPLVRNVFSACIWIPY
                     FIVSERVKRTFVK"
     gene            872..1782
                     /locus_tag="RPM73_004444"
                     /pseudo
     CDS             872..1782
                     /locus_tag="RPM73_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
BASE COUNT          499 a          319 c          480 g          484 t
ORIGIN      
        1 agcagggatt ggtatttgga taaaagcaaa gttacaagaa agaaaagacg ataaaaaata
       61 ggcttacggt aacttcaact ttggggaatt tttattggta gttatgttct tggtcaatgc
      121 catcaggaat gaggtaatgt gcgtattacc gtggaagtga tcggtgagta actccaatct
      181 caatagaggt tattgggcgc tctccttcca tgaaaacacg caacctaatc ggggaccggg
      241 gcatatgctg actggtgaca atacagatga aaataaacag catgatcttc aacttttccc
      301 gtataaataa taggtgatta tcagatgagc catgatgcag agaagaaaat aagactttgt
      361 attcaatgtg agaatgaagc actgaaggac agtgactttt gcgctgtttg tgaagaacgc
      421 gaatttaaaa aaattcgtgg ttggctatac ttgcctgcaa taggactggt tttaacaatt
      481 ctcgctaata tgatctctat taatttcact atacgattct taatggaaaa tattgcgcag
      541 atcggtcacg cacaaaaggg gattctcttt tttgaactct ttgctttcat tggtatgttt
      601 tcctatggaa ttttcgttag tagtttgttt tttcgtaaaa agcgtcaatt gcctcatcac
      661 tatattgtac ttgttgggat aggaacgata tttgtcgctg ttgatttact gctgggccac
      721 atctatttag acgtgcctta tgtttttgat acggtaaaac cattggtacg taacgtgttt
      781 agtgcctgta tttggatccc ctattttatt gtatctgagc gtgttaagcg gacatttgtg
      841 aaataatttg tccgtcagat gtcccacatg agctcatgca gtggtgaatg ccacgctggc
      901 ggtggtacag gaaaatagcg cccttgccag cgcagcaacg ggagaattgg tagccagagc
      961 gattgctgga atgttgtatc cgggcgtgaa gcagtcagac ctcagtgaag agcagaagca
     1021 gacgataagt acgctggcaa ctgtgtcagc agggctggcc ggcggtctaa ccggaaacag
     1081 caccgcatca gcagcagtgg gagcacagag cgggaagaat gctgctgaga ataacctggt
     1141 tggtggcaca gagtccgagc agagcaaagc tgctcagatg catggcgcag atgtactttc
     1201 ctgtaacgat gccccaggcg gtgaagcctg taagcgagga gaagcagcca ataaagcgta
     1261 tgccggagcg ttggccactg gtagtgtagc tctcttacct ggcggtgctc aggccatgtg
     1321 gggattaggg gcaggagcta atgcaggtgt tagctatctg acggatggaa ctatagatcc
     1381 tacaacagcg attatcggtg gttgggttaa tgtgattagt gcagggaatg gagttgtagg
     1441 cacagttggc tggaatgcaa ctggaggagc attaggaaac tggctcgaag gaaaagcccc
     1501 aacatctggt gcgttgatca atggtgctgg ttctgctatt ggatatggca ttggtaaagg
     1561 cctttcatgg ggcattaata gtggtgctaa ctggttaaaa gacggctggg acccgaaatt
     1621 taatccaaca cttcaaaaat ataccgatat taaaggagaa ttggggctat caaaagaaat
     1681 gacacccagt aggattcctg gtatagctgg ggatgtgtcg gcttcttttg cttctgagta
     1741 tgaaggcaaa aagattgagt cttacattcc ggaggtgaaa aa
//
LOCUS       DAPPNJ010000351         1773 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000351 DAPPNJ010000000
VERSION     DAPPNJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.353"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1376
                     /locus_tag="RPM73_004445"
     CDS             <1..1376
                     /locus_tag="RPM73_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX6522697.1"
                     /translation="GRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEP
                     LVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRI
                     QTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRL
                     ESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVST
                     EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPD
                     IAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGM
                     DTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQR
                     TSRGIRK"
     gene            1373..>1773
                     /locus_tag="RPM73_004446"
     CDS             1373..>1773
                     /locus_tag="RPM73_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522698.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKS"
BASE COUNT          505 a          413 c          485 g          370 t
ORIGIN      
        1 acggcaggct gacggagaaa accgacctca tcccggaagg ggttatccgc acggatgatg
       61 agcggactca ccggtaccat tacgacagtc agcaccggct ggtgcactac acgcggacac
      121 aatatgaaga gccgctggtc gaaagtcgct atctttacga cccgctgggc cgcagggtgg
      181 caaaacgggt atggcgacgt gaacgggacc tgacgggctg gatgtcgctg tcacggaaac
      241 cgcaagtgac ctggtacggc tgggacggcg accggctgac cacgatacag aacgacagga
      301 gccgcatcca gacgatttat cagccgggga gcttcacgcc actcatcagg gttgaaaccg
      361 ccaccggtga gcaggcgaaa acgcagcgcc gcagcctggc ggataccctt cagcagtccg
      421 gcggcgaaga cggtggcagt gtggtgttcc cgccggtgct ggtgcagatg ctcgaccggc
      481 tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg ctggcatcgt
      541 gcggcctgac ggtggagcag atgcaaaacc agatggaccc ggtgtacacg ccggcgcgaa
      601 aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcgcttgtc agcacggaag
      661 gggcaacaga atggtgcgca gaatacgatg aatggggcaa cctgctgaat gaagagaacc
      721 cgcatcagct gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc
      781 tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gaggtatatc actcaggatc
      841 cgattgggct gaagggggga tggaattttt atcagtatcc gctgaatccg gttcagtata
      901 tagattcaat gggactggca tcaaaatatg gacacttaaa taatggcgga tatggagcga
      961 gacccaacaa accgcctacg cccgatccaa gtaaattgcc ggacatagcg aaacaattaa
     1021 gactgccata tcctattgac caggccagta gtgcgcctaa tgttttcaaa acattcttca
     1081 gagcattaag cccttacgac tacacactgt attgcaggaa gtgggtaaaa ccaaatctga
     1141 cttgtacgcc acaggatgat tcccagtatc cagggatgga tacaaagaca gcaagtgatt
     1201 acctgccaca gacaaattgg ccaacaactc aattaccacc aggatatact tgtgcagaac
     1261 cctatttatt cccagacatt aataaacccg atgggccagc aacagcaggg atagatgatt
     1321 tgggtgaaat tttagctaag atgaaacaga gaacatcgag aggaataaga aaatgaaaag
     1381 agttttgttc tttttgctga tgatatttgt tagttttggt gttatagctg attgcgaaat
     1441 acaagctaaa gatcatgatt gttttactat tttcgctaag ggtactattt tttccgcgtt
     1501 tcctgtctta aataataaag ccatgtggag atggtatcag aatgaggaca tcggtgagta
     1561 ttattggcaa acagaactgg gtacatgtaa aaataataaa ttcacaccaa gcggggcgag
     1621 actcttaatt cgcgtcgggt cactacgcct taatgaaaat catgccatca aaggaacgtt
     1681 gcaggagttg ataaatacag cagaaaaaac ggcgtttctc ggcgatcggt ttagaagtta
     1741 tataagagcg ggtatctatc aaaaaaagag cag
//
LOCUS       DAPPNJ010000352         1756 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000352 DAPPNJ010000000
VERSION     DAPPNJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.354"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..526
                     /locus_tag="RPM73_004447"
                     /pseudo
     CDS             1..526
                     /locus_tag="RPM73_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            554..1512
                     /locus_tag="RPM73_004448"
                     /pseudo
     CDS             554..1512
                     /locus_tag="RPM73_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1524..>1756
                     /locus_tag="RPM73_004449"
     CDS             1524..>1756
                     /locus_tag="RPM73_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX6522699.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQ"
BASE COUNT          552 a          326 c          410 g          468 t
ORIGIN      
        1 gcgttcgagc tacggcaatc gagtcaacgg ctcagcgatg caagaaaaac ccaatttatt
       61 cacctaaaat agtagaacaa tctcaaatta ctgcttttga taaatcgact aatgtcagac
      121 gtgctacagc atttgctatt tctgttatca atgataaagc aacaattcca ctattgatta
      181 atctgttaaa agatccaaat ggagatgtca gaaactgggc cgcatttgca ataaatatca
      241 ataaatatga taatagtgat attagggatt gttttgtgga gatgcttcag gataaaaatg
      301 aggaagtccg tattgaagca ataatcggac tttcctacag aaaagataaa agggttttat
      361 ctgttttatg cgatgagtta aaaaaaatac tgtttatgat gatatcattg aagctgcggg
      421 tgaattaggt gataaaacgc tacttcctgt tttagatact atgttgtaca agtttgatga
      481 caatgaaatt ataacttccg ctattgataa gctgaagcgt tcatagcgtg agttgcctat
      541 gcacagtggg ggattcccgc cggcacggtg cagatgctcg accggctgga aagtgaaatc
      601 ctggctgacc gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg
      661 gagcagatga aaaaccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac
      721 cactgcgacc atcgcggcct gccgctggcg ctcatcagca cggaaggggc aacagcgtgg
      781 tgcgcagaat atgatgaatg gggcaacctg ctgagtgatg agaacccgca tcatctgcag
      841 cagctcattc gcctgccggg tcagcagtat gatgaggagt ccggcctgta ttacaaccgc
      901 caccgctatt atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag
      961 gggggatgga atttttatca gtatccgttg aatccggtca taaatgtaga tccgcaaggt
     1021 ttggttgata taaatttata ccccgaaagt gatcttatcc attctgtagc tgatgagatt
     1081 aatatcccag gcgttttcac aatcgggggg catggtaccc ccacatctat tgaatccgca
     1141 acgcgcagta tcatgacagc taaagatcta gcatatctaa ttaaatttga tgggaattat
     1201 aaagatggga tgacagtttg gttattttct tgtaatacag gtaaaggaca aaattcattt
     1261 gctagccaat tagctaaaga gttacataca aatgtaatag gacctgacac gctatggacg
     1321 tggtgggggc gaggaactaa tggtaagtta aaaatggata cagtgctaac agcaccaacg
     1381 aaccttaatt caaataagga tctaatggct ataacaacaa aagaccttgg taattggata
     1441 acatatgggc catctgggca ccccatttct aatatgcaag gtacgccaga aaaacccagt
     1501 gatataagat aggttgtaga tgtatgaaag catgcttgtt actatttttt tatttttctc
     1561 ttattggtca attgcatggt gctgatgtga aaataaaaga aaacgaaagt gtgatgggat
     1621 ctacagcaat gacctatgat ttaagtgaag aaaagctgat gaaactgaag tataaatcac
     1681 aacatggtga ttcagaggca tcatttcgac tatatcaata ttactgcttt actaaaaata
     1741 atattgataa acaatt
//
LOCUS       DAPPNJ010000353         1733 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000353 DAPPNJ010000000
VERSION     DAPPNJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.355"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RPM73_004450"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RPM73_004450"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDX6522700.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          412 a          418 c          434 g          469 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacgacgtc acctttgtgt gccagaccgc tctgcagagc
      181 cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgagtagc
      361 aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc
     1021 tttgttttcg cccaggtcac cgttgttcag cactttacag ataactttgt taccttcaat
     1081 ggcggtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caactgacag
     1141 gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtggtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgc
//
LOCUS       DAPPNJ010000354         1725 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000354 DAPPNJ010000000
VERSION     DAPPNJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.356"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(103..546)
                     /locus_tag="RPM73_004451"
     CDS             complement(103..546)
                     /locus_tag="RPM73_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDX6522701.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            703..1632
                     /gene="metA"
                     /locus_tag="RPM73_004452"
     CDS             703..1632
                     /gene="metA"
                     /locus_tag="RPM73_004452"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDX6522702.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          412 a          481 c          396 g          436 t
ORIGIN      
        1 aggcccagtc ggaagactgg gcctttttgc gctggtgcag aaaaaggtcg gatgcgacgc
       61 tggcgcgtct tctccagcct gggcaggcac ggcaggggcg gcttattccc ccacatacgc
      121 cagattcagc aacggatacg gtttccccaa atcgtccacc tcagagcgtc ccgtaacctt
      181 aaaacccacc ttcttataga acccaaccgc ctgctcattt tgctcattaa cgttggttgt
      241 cagttccggt gccatcgaga gcgcatgctc caccagcatc cgacccacgc cgcagccgcg
      301 cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca taaacccaac
      361 cggctgatcc cgttcattaa ccgcgaccca caacggtgct tccggcagaa aggaacgaac
      421 cagctcttcc agctcggccc gatactctgc tgataaaaaa tcgtgggtgg catcgacaga
      481 tcgacaccaa atcgcaacga gttcctcccc ttcctcatgc tgtgagcggc gaatactaat
      541 aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc cttgaaactt
      601 tctcaccttc aacatgcagg ctcgacattg gcaaattttc tggttatctt cagctatctg
      661 gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgcctat tcgtgtgccg
      721 gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat gacaacttct
      781 cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga tccttaacct gatgccgaag
      841 aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt gcaggtcgat
      901 attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga gcatctgaac
      961 aacttctact gtaactttga agatattcag gaacagaact ttgacggtct gattgtaacc
     1021 ggtgcgccgc tgggcctggt ggagtttaat gatgtcgctt actggccgca gatcaaacag
     1081 gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg ggcggtacag
     1141 gccgcgctca atatcctcta cggcattcct aagcaaactc gcaccgacaa actctctggc
     1201 gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt tgatgattca
     1261 ttcctggcac cacattcgcg ctatgctgac tttccggcag cgttgattcg tgattacacc
     1321 gatctggaaa ttctggcaga gacggaagaa ggggatgcat atctgtttgc cagcaaagat
     1381 aagcgcattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct ggcgcaggaa
     1441 tatttccgcg atgtggaagc cggactaggc ccggaagtac cgtataacta tttcccgcac
     1501 aatgatccgc aaaatacacc gcgagcgagc tggcgtagtc acggtaattt gctgtttacc
     1561 aactggctca actattacgt ctatcagatc acgccatacg atctacggca catgaatcca
     1621 acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct taactcaggc
     1681 accttcgggt gcctttttta tttccgaaac gcacctcagg aggtg
//
LOCUS       DAPPNJ010000355         1704 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000355 DAPPNJ010000000
VERSION     DAPPNJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.357"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            166..381
                     /gene="essD"
                     /locus_tag="RPM73_004453"
     CDS             166..381
                     /gene="essD"
                     /locus_tag="RPM73_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDX6522703.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            386..697
                     /locus_tag="RPM73_004454"
     CDS             386..697
                     /locus_tag="RPM73_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDX6522704.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            694..1227
                     /locus_tag="RPM73_004455"
     CDS             694..1227
                     /locus_tag="RPM73_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDX6522705.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1224..>1704
                     /locus_tag="RPM73_004456"
     CDS             1224..>1704
                     /locus_tag="RPM73_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDX6522706.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     "
BASE COUNT          482 a          349 c          436 g          437 t
ORIGIN      
        1 tcgtattatt attttatata tgactttctt tcaaaataat tacccacatt tttaatgtgt
       61 atgttttttt agcgccgttg agaacaacgt gtgctgtcaa aactaccccg tagactccga
      121 tcttttcaaa catattgcac catccgtgta catcggggtg aggatatgaa atcaatggat
      181 aagttaacaa caggtgttgc ctatggcaca tcggcgggta atgctggttt ctgggcattg
      241 cagttactcg ataaagtaac tccgtcacag tgggctgcaa tcggtgtgct gggtagcctg
      301 gtttttggcc tgctgacgta tctgacaaat ctttatttca agattaaaga agacaggcgt
      361 aaggctgcga gaggagagta atccaatgac tcaagactat gaactggttg tgaaaggagt
      421 ccgtaatttt gagaataaag ttacggtaac tgtagcctta caggacaaag aacgctttga
      481 cggtgaaatt tttgacctgg atgtcgccat ggaccgtgtt gaaggagctg cgctggagtt
      541 ttatgaggca gcagccagaa ggagcgtccg gcaagtcttc ctggaagtag cagaaaaatt
      601 gtcagaaaaa gttgagtctt atctgcagca tcagtactcc tttaagattg aaaatcctgc
      661 caataagcac gagcgtcctc atcataaata tatatgaaca caaaaatcag atacggcctg
      721 tcggctgccg ttctggcgct gattggtgct ggcgcatctg ctcctcagat acttgaccag
      781 tttctggacg aaaaagaagg taaccacaca atggcatacc gcgatggttc tggcatatgg
      841 accatctgtc ggggtgccac agtggtggat ggaaaaaccg tttttcccaa tatgaaactg
      901 tcgaaggaaa aatgcgacca ggtcaacgcc attgagcgtg ataaggcgct ggcatgggtg
      961 gagcgcaata ttaaagtacc actgaccgaa ccacaaaaag cgggtatcgc gtcattttgt
     1021 ccctataaca ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gctgaatgct
     1081 ggtgatcgta aaggtgcatg cgaagcgatt cgctggtgga ttaaggatgg cggacgcgat
     1141 tgccgcattc gttcaaataa ctgttacggt caggttattc gtcgtgacca ggagagcgca
     1201 ttaacctgct gggggataga acagtgaatc agatattcat ggtgattttt ctcgtgttgt
     1261 caggatttat cgtcggaaat gtctggagcg acagaggatg gcaaaaaaaa tgggcggaac
     1321 gtgatgctgc cgcattatca caagaggtaa atgctcaatt tgctgctcga ataattgaac
     1381 aggggcgaac tatagcccgt gatgaggctg ttaaagatgc acaacagaaa tctgctgaaa
     1441 tttctgccag ggctgcttat ctgtctgata gtgttaacca gttgcgtgcc gaagcaaaaa
     1501 aatatgccat acgccttgac gcagcgaagc ataccgcaga tcttgccgct gccgtcagag
     1561 gcaaaacaac caaaaccgcc gaaggaatgc tcaccaacat gctcggagat attgcagcag
     1621 aagctcagct ttatgctgaa attgctgacg aacgctacat cgcaggagtg acttgtcaac
     1681 agatctatga atctttaaga gata
//
LOCUS       DAPPNJ010000356         1699 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000356 DAPPNJ010000000
VERSION     DAPPNJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.358"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(2..1285)
                     /gene="ydfJ"
                     /locus_tag="RPM73_004457"
                     /pseudo
     CDS             complement(2..1285)
                     /gene="ydfJ"
                     /locus_tag="RPM73_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          497 a          426 c          331 g          445 t
ORIGIN      
        1 tttacagact tctggaaggt tgcgcagctc ttacaacacg cggttgatct tccgcagcgt
       61 cttccagcgc acttaaatca cggtctttca cctctggcat tttcagcgca gagattaaac
      121 caatcactga atatgccatg atcataatgg cgatcggata ccaggattcc gtcatggtgc
      181 agaaaatacc cgccaggata ggaccaaaac cggaagcgat aagaccacca atttctttag
      241 aaatagccat ccgggtaaag cggtttttac agccgaacat ttctgccatg gtaatgtttt
      301 ccagagcaaa taatcccagc accgcacagt tatgaatcac aatcagtgca accataatgg
      361 tgctcggggc atagctttta tctacaatga tagaaagcat tggccatgcc agcacaatcg
      421 cggaggtatt cataataata tacgggatcc ggcgaccaat tttatcggat aaccaaccaa
      481 ggaacggaat ggtcataaag ccgagaatcg aactaatcat caatgcatct gttggaattg
      541 ctttgttaaa caataacgtc tgcactaaat agcctgcaag gaaagtctga attaacccgg
      601 agttacccgc ctgaccaaaa cgcagccctg ttgccagcca gaaggatttg ctctggaaca
      661 tgctaccagc aggtgcaggt tttgctgtgg gttggttgct gtcattaacc ttctcaaaga
      721 ccgggctttc tttcagattc atacgtaacc agatagcaaa gaccatcacg acaacgctcg
      781 ccaggaacgg tatacgccat ccccacgcca gcagttcctc tttactgaga atgaagaaca
      841 taaaggccca gattgccgtt gcgctcaagg ttccgcagtt agttcccata gctacaaatg
      901 aggagataat tccgcgctta ccttttggtg catattccgc cagcatcgta ccggcaccgg
      961 aaatttccgc acctgcaccc aacccctgaa taatacgcag cgtcaccagc aagatggggg
     1021 caaaaacacc aatctgtgca taggtcggta acacaccaat taaggtggta cagatcccca
     1081 tcatggtgat ggtaataaag agcacttttt tacgccctat tctgtcgccc attttgccga
     1141 aaataaatgc tccgacaata cgcgccacat aacctgcacc gtaggttccc attgccaaaa
     1201 ttaacgccat tgccgttgat gattcaggaa aaaatatttc atgaaacact aacgctgcgc
     1261 cgagcgaata taactggaaa tccataattc acaggtgttt tttcccatcc tgtggtttcc
     1321 ttggcgtttt ctaggttttt tcagatagtt gcatttttta aaaagcatcc taagttcgat
     1381 ctcagtgtct atctggggcc tatttctgtc ccatatatgc cccaaaaaaa actccccaac
     1441 agataagtag ttttttcatg gatttatgcg taaaatcaag aacggctgga aatcattcaa
     1501 tactcacact atcgaaaaat ttaccagcca accgcagcac gttcttgcat aaggcgtgtc
     1561 tgcggttttt caactattca gatacatcac tcccatcaca ttcattcctc cgcatcaaag
     1621 gcatataggc tatatcacct tgatattttt atttttcaga taaaaactgt tatctatgta
     1681 tacttttaaa cccaatccg
//
LOCUS       DAPPNJ010000357         1691 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000357 DAPPNJ010000000
VERSION     DAPPNJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.359"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..439)
                     /locus_tag="RPM73_004458"
     CDS             complement(<1..439)
                     /locus_tag="RPM73_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDX6522707.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFAL"
     gene            complement(448..1215)
                     /gene="sctT"
                     /locus_tag="RPM73_004459"
     CDS             complement(448..1215)
                     /gene="sctT"
                     /locus_tag="RPM73_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDX6522708.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1217..1477)
                     /locus_tag="RPM73_004460"
     CDS             complement(1217..1477)
                     /locus_tag="RPM73_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDX6522709.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1487..>1691)
                     /locus_tag="RPM73_004461"
     CDS             complement(1487..>1691)
                     /locus_tag="RPM73_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDX6522710.1"
                     /translation="IIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFV
                     AMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          594 a          344 c          287 g          466 t
ORIGIN      
        1 ccaatgcaaa aaaaacaata taaagaattg ctttaacaaa ttcttttacg gtttttaagc
       61 caaatatacg ctttaagcca tttactggat tcaatgaatc aaacttaaat ttaacagctt
      121 cagttgctaa ttgcatttta ctttgaaacc aggagacaag tattgttgtg aacatgcaaa
      181 ccagcaaaaa aggaatgttc gttttcaacc aaccaacccc catagcttta aaatagtccc
      241 aaatatctgg tttagcgtta tgatcaagga tgtattcaag aatcgacata atgtgatgca
      301 catcaaaaac atatccgaga tataaagtac ccacaagcat tattacagag atggttaagt
      361 ctctactttt tagaatttgc ccttttttag aagcatcctg gagtttcttt tgtgttggct
      421 tttcagtttt attggccatg aaacactcta tttctcaatg tatagttgca attgttctgg
      481 gaaaaaggat aacggcatta ctcgttcagc cagtattggg gcaaaataaa taattaatat
      541 taaaaatgcc agcgcacttt tgacagtcag tgaaatggca aaagcattta gttgtgaagc
      601 ataacatgcc agcaatccca ggacagcctc accgcccagc ataacggcaa tcacggggct
      661 tgcgtaaacg atagtatgag tcattatgtt attaatatat gagaataaag gctctaattt
      721 aggaaaatta aaattgcctg aaggccataa attataactt tgccaaagag tttccagaat
      781 aaagttcatt ccacctttag taagataaac cgcagcagag aaaaggttaa ataatcttgc
      841 taattccgag gtatcgacac ctgtagccgg atccaaagta ctacttaatg ttgcaccgcg
      901 ttggttatcg ataaagctac ctatagcaag gaaaatccaa aacggggatg ctaataagca
      961 tgcaataaac agccctatga taatttctct ggcagcgaac aaagcaatat cgatttcatt
     1021 taagttcaat aattcatagt ggtaatgctg aactaatgca catgatacca ctattattat
     1081 tgggtgacga actactgttg gtatattccc actggcgaaa aatggtaaca aatagaaagt
     1141 tggtgccaat cggcaaaaac cgagcgctgt agcagccagc agactatgca actgatagag
     1201 tatcgcctcc cccatgctat cctctcgcga atgctaaatt cagcatttcg ataccaaatg
     1261 aatataattt ttcaccatac cacccgctca ttaaaaaaaa gcaaatactt acgcacaata
     1321 atttcacgcc aaatggcaac gtctgttctt gcaactgcgt taccgtttga aaaagcccta
     1381 ccaataaacc aacaaatgtc gcaactgcaa taggtcctgc agacataact aaaatcagat
     1441 acagcgccct attccccgca aaaacaatat cgtccatatt ttttcctcag ggattaatgg
     1501 ataggtcaaa atattgtaaa atgagccctt ttgataacat tgtccaaccg tccatcgcga
     1561 caaacaaaat taattttatc ggtgtagata tggttaccgg actcatcatc atcataccta
     1621 acgttaacag aacgctggat ataaccagat ccactacgac aaagggtaaa tagatataaa
     1681 aacctataat a
//
LOCUS       DAPPNJ010000358         1689 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000358 DAPPNJ010000000
VERSION     DAPPNJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.360"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..615)
                     /gene="trbL"
                     /locus_tag="RPM73_004462"
     CDS             complement(<1..615)
                     /gene="trbL"
                     /locus_tag="RPM73_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HDX6522711.1"
                     /translation="MRIVSKLTFWGMIFLFSVNAYAGQLDSSGLLDTLLDKFQQVAST
                     WSLVIGDYANWLFWGLVLISMVWTFGMLAMQGEGLINALAEIVRFFAVIGFFYYLLIN
                     GPAIAQSIINSMRQLAANALGTSTGISPSSIVDIAFVILTKVSSAASIWSPMISTIMI
                     TVAIIVLVVMALIAINMLIMLVSAWVLCYAGVIFLGFGGSKWTSD"
     gene            complement(803..1597)
                     /gene="trbJ"
                     /locus_tag="RPM73_004463"
     CDS             complement(803..1597)
                     /gene="trbJ"
                     /locus_tag="RPM73_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDX6522712.1"
                     /translation="MKLMLLVFSSLRGILAAKKSLAASFLASIMALPATAGIPVIDGT
                     NVVQTTISAVNNVQAVAKQIQQYQTQLQQYENMLKNTVAPAAYIWDQANSTINKLLQA
                     QDTLNYYKNQAGSLDSYLKRYQDVNYYRSSPCFNSNVECTADEISALRKAEQNNSEAR
                     KKANDAVFKALDQQQETLQSDADNLTDLQTQATGAQGQMEAIQAANQLASAQTNQLLQ
                     IRSLLIAQQNAAATLALAQTDKEAQQIVADEKALAGENTPSPKRIW"
BASE COUNT          473 a          375 c          333 g          508 t
ORIGIN      
        1 atcagaagtc catttcgagc caccaaaccc aagaaaaata actcctgcat aacacaacac
       61 ccatgcagaa actaacatta ttagcatatt tatcgcgata agcgccatta ctactaatac
      121 tattatcgcg acggttatca ttatggtcga aatcattgga gaccaaattg atgccgctga
      181 actgaccttt gttaatatca caaaagctat atcaactatg ctggagggtg aaataccagt
      241 acttgttcct aaagcatttg ctgcaagttg tctcattgag ttaattatag attgtgctat
      301 ggctgggccg ttgattaaaa gataataaaa aaaacctatc acggcaaaaa aacgaactat
      361 ttctgcaagt gcattgatta aaccttcccc ttgcatcgcc aacataccaa atgtccaaac
      421 catactgatt aataccagcc cccagaagag ccaattcgca taatcaccaa ttactaatga
      481 ccatgtgctg gctacttgct gaaacttatc taataacgta tcaagtaaac cgctactatc
      541 taattgacca gcataagcat ttacagaaaa caaaaaaatc attccccaaa aagtgagttt
      601 gcttactatt ctcataatta ccattctcgc ttagggctag gattgttatt actgcgcagg
      661 gcacatttat cgtagctatg ttcgttcaat ttattctctg gcgtcgataa acaatcgtca
      721 tcttttttat cacaagagca tagaatcaat ggaatgatta gaataaaaat gaccaaaagt
      781 ttatctttca ttcagctatc cctcaccaaa ttcgcttcgg gcttggtgtg ttctcgccag
      841 ctaacgcttt ttcatcagca actatttgtt gggcctcttt atcggtctga gccagcgcca
      901 gtgttgctgc agcgttttgt tgagctatca gaagcgagcg gatttgcagc agctggtttg
      961 tttgagcact ggcaagctgg ttagcggcct gaatagcttc catttgccct tgcgcgccgg
     1021 ttgcctgagt ttgcaaatct gtcaggttat cagcatcgct ttgcagtgtt tcctgctgtt
     1081 gatcgagagc cttaaacacg gcgtcgttcg cctttttacg tgcctcagag ttgttctgtt
     1141 cagctttacg cagtgcgctg atttcatccg cagtacattc aacattactg ttaaagcacg
     1201 gcgatgagcg gtagtaattc acgtcctgat aacgtttcag atatgaatcc agactaccgg
     1261 cctgattttt gtagtaatta agggtgtcct gcgcctgcaa caatttgttg atggtcgaat
     1321 tggcctgatc ccagatatag gctgcaggcg cgactgtatt tttgagcatg ttttcatact
     1381 gctgcagctg cgtctggtac tgctggatct gtttagccac agcctgaacg ttattgactg
     1441 cgctgattgt ggtctgcacc acattagtac catcgatgac agggataccg gctgtcgccg
     1501 gtaatgccat gatggatgcc aggaaggaag ctgctaaaga ttttttagct gctaaaatcc
     1561 ctctcagtga tgaaaatact aggagcatta acttcatttg cccaccttaa ttttaactga
     1621 ttattgatat tgaaaactcg tacttttaac agatacagat atttaggaag ccataaattt
     1681 atttatcca
//
LOCUS       DAPPNJ010000359         1670 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000359 DAPPNJ010000000
VERSION     DAPPNJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.361"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1245)
                     /gene="yiaN"
                     /locus_tag="RPM73_004464"
     CDS             complement(<1..1245)
                     /gene="yiaN"
                     /locus_tag="RPM73_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDX6522713.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPD"
     gene            complement(1248..1664)
                     /gene="yiaM"
                     /locus_tag="RPM73_004465"
     CDS             complement(1248..1664)
                     /gene="yiaM"
                     /locus_tag="RPM73_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDX6522714.1"
                     /translation="MFINIILRYGFQTSILSVDELSRYLFVWLTFIGAIVAFMDNAHV
                     QVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLM
                     YAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT          524 a          420 c          409 g          317 t
ORIGIN      
        1 atcgggaata aaaacaaata cgactaataa tgaatataaa accaggacgt aagggaaaac
       61 gcctctgact gcatcatcga atttgagttt tgccacccct gaaataacgt taagtacatt
      121 accgataggc ggtgtaatta agccgattga gcagttaatg ataaacatga caccgaaata
      181 aatcggatcg attcctgcct ctttaactaa aggcattaat accggggtaa gaattaatac
      241 cgtcggcgtt aaatccatga ccatgccgac aatcaggata gccaccataa tgacgataaa
      301 cagcagacgc ggtgaatcga ccaacggttg cagtaaatca gaaaccatca tcggcagttc
      361 agcaatggta atcagccagg cggaaacttg tgccgaggcc accagaaaca taaccactga
      421 ggtggttttc gccgcgccaa tgagtacatg ccaaagtgtg gcgaaggtca tttcacggta
      481 aataactgtg gcgacaaaca gcgcatagaa agcagcaacg gccccggctt cggtaggggt
      541 aaacagaccg gagcgaaacc cgccaataat gatgactgga agaaacagcg cccagatacc
      601 ggagacaaaa gagtgccaga tttcctgcat cgttgctttt tgctggcggg gtaaattaag
      661 gcgactggcc tgccaccacc aggtaagcat cagcgttgcg cccatcatca tgccaggggc
      721 aatgcccgcc ataaacagct tactgatgga taatccgctg gaaacgccga agataataaa
      781 cggaatggaa gggggaataa ttggcgcgat aatgccgcca gaggcaatca gccccgccgc
      841 ccggttgacc gggtagttgg cgctgcgcat catcggcacc agtagagcgg caacggcggc
      901 ggtatccgca acggcagaac cagaaaggct cgccataatc atagctgcca gcacgccgac
      961 gtagcccagc ccgcctggtt tatgccccac cagtttcatc ggcaggtcaa caatgcgttt
     1021 tgacaagccg cccgcattca tgatttcacc cgccagcaca aagaacggaa tcgccagcag
     1081 ggagaagcta tcggctccgt tcaccagcgt ttgtgccatg atctggacat caaacatgtc
     1141 cagccagaac attaacgccg ccccgcacaa caacagtgcc caggcaatag gcaaaccgat
     1201 agcaatacca cccaacagac agcccagaaa aatcagcaca gccatgatta agctccttgc
     1261 ggtggcgacg ttagagaatt gctacgcgtg atgagttgat ataaatgacg cagttcaaag
     1321 aatgcgataa cgaggctggt gggcagacaa gcggcataca tcaggccgat gggtaaaccg
     1381 aggatcggtg aataatcgct ccagtcctga attgttttta gcgttgcgcc ccaggccagt
     1441 gcgccacaaa taaataagat taaggaatgg gtaaccagag cgactcgtcg ttgccatgcg
     1501 ggggagagtt tctccaccag aaaggtgacc tgaacgtggg cgttatccat aaaagccaca
     1561 atcgcgccaa taaatgttaa ccagacaaat aaataacgtg acaattcatc tacagataaa
     1621 atgcttgtct gaaaaccata tcttaaaata atgtttataa acacaataca
//
LOCUS       DAPPNJ010000360         1670 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000360 DAPPNJ010000000
VERSION     DAPPNJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.362"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..1267
                     /gene="speC"
                     /locus_tag="RPM73_004466"
     CDS             <1..1267
                     /gene="speC"
                     /locus_tag="RPM73_004466"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX6522715.1"
                     /translation="DYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHK
                     QQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHE
                     GVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQ
                     FVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGV
                     VPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERY
                     AGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRL
                     PDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYI
                     EEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1264..>1670
                     /locus_tag="RPM73_004467"
     CDS             1264..>1670
                     /locus_tag="RPM73_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6522716.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICT"
BASE COUNT          402 a          400 c          437 g          431 t
ORIGIN      
        1 tgactacatt ctgtttgact ccgcatgggt tggctatgag caatttatcc cgatgatggc
       61 tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca
      121 atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag
      181 ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat
      241 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
      301 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc
      361 ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga
      421 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca
      481 gtttgtacca ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt
      541 cgatccttgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga
      601 agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc
      661 ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa
      721 gcttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc
      781 attagcagaa gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
      841 cctgcgtcag ttgtgtcaag aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact
      901 gcaaaaagag atgttccgta aggaacactt cccacgcgta agcatgaatc cgcaagaagc
      961 caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg
     1021 tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgcgttg ttccgggtga
     1081 aatctggggt ggtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgtt
     1141 gccaggtttt gcaccggagc ttcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
     1201 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa
     1261 attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg
     1321 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca
     1381 atttcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
     1441 gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg gcggttatgc ggagtatgct
     1501 ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcgct gctgattgct
     1561 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
     1621 tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac
//
LOCUS       DAPPNJ010000361         1627 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000361 DAPPNJ010000000
VERSION     DAPPNJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.363"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..105)
                     /locus_tag="RPM73_004468"
     CDS             complement(<1..105)
                     /locus_tag="RPM73_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDX6522717.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRL"
     gene            complement(161..472)
                     /locus_tag="RPM73_004469"
     CDS             complement(161..472)
                     /locus_tag="RPM73_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDX6522718.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(500..>1627)
                     /locus_tag="RPM73_004470"
     CDS             complement(500..>1627)
                     /locus_tag="RPM73_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDX6522719.1"
                     /translation="LHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISY
                     EQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRL
                     ARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAGLNDLIYTLI
                     QTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHG
                     TTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNI
                     NEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAF
                     LGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          507 a          363 c          341 g          416 t
ORIGIN      
        1 aaggcgctgc tctatggtta attttgttgc catcgcgttc aatggaaaca atgctgtaat
       61 aatagaaaaa gacaaaaggc tgacgttaag cgtctttata ttcatgactt atccttaaga
      121 gtataataat ttcctccagc tatcgcactg gaggaaaaaa tcaggcatgg agttcttttc
      181 ttaatgaaat aatttccctg gctaaatcct ggcataatat tgcattcatc aaatgatcct
      241 gtgcatgaat cagaattaaa ttaactttta ttttgccaca gccttcatcc tgactaatta
      301 atgcggtctg aatcttatgt gcctgaagac aggcagattc agaactggcc attagctgag
      361 ttgcgccctg ccagtctttt tgcccggcac aacgtatggc ttccatcgcg tcagaacgtg
      421 cctgccccgc gttgaccagt aactccataa caagttcttc atctgcaaac atcacaatct
      481 ccttcctggg taaatggaat tatccgttag ttgtcatgtt attgactttg ttggcggcaa
      541 taacaaaagg taaatagatc attgtcgcca cacctaaaca taaaaaacca accagtaaag
      601 ccagccagtt ccccccggtt ccgaggaaag caattaaagg tccgggcgta gtccagggca
      661 cggcataagc aaccggtgga ataatttcca tcgaaacaaa gaagtaacca atggcacagt
      721 taacaatggg aaccataata aacggcacca tcatgatggg gttaagtaca ataggaaggc
      781 caaacattat cggttcattg atattgaaaa taccggggat aaatgacatt tttgccagat
      841 cacggtattc cgcacgctta gaagcgataa aaatagccaa caataacccc aacgtcatac
      901 ctgagccgcc gcagttggcg aatgcatcat taatagaagt ccaggtaatc ggatatggcg
      961 cgccccaggt agtgccatgt tgagcggccc aggagagatt ctccagatta gcctcagaaa
     1021 aaacggtttc tcgaattgcc gatgtggtat taggtccgtg gatccccagc acccacagga
     1081 aattacccac agcccccagg ataattaccg caaagatatt cgttcccata tgtttgagcg
     1141 gcgtctggat taatgtgtaa ataagatcgt ttagtcctgc cggggatatc agtgccaggc
     1201 aataattaag tgcggaaaag aacaccatga caaaaaatat tggcaataaa actttaaatg
     1261 aacgcgctac cgcaggcggt acagctgccg gcatcgttat ggtgattttt gggtttcgag
     1321 ctaagcgaca aaatatttca ctggtgatca atgcaacgat aacagcaaca aacaacccct
     1381 gcgcaccgag atatttggtc gtcaggaatg cttgcccatc tatcatctga tatggcgtat
     1441 aaacaataaa aaatgctcct attgccgtta atccgcataa aagatcatct tgctcatagc
     1501 taatcgccat attcctggcg accaggaaag caatcataat tgacatgata tttacagaac
     1561 cattcattac cggagtaaaa atagcttgtg ctttttcaag gttggggaaa atgctattca
     1621 gatgcag
//
LOCUS       DAPPNJ010000362         1594 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000362 DAPPNJ010000000
VERSION     DAPPNJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.364"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..382
                     /locus_tag="RPM73_004471"
     CDS             <1..382
                     /locus_tag="RPM73_004471"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDX6522720.1"
                     /translation="IRNEEGKALLGQRLNRPAQNYWFVPGGRILKDESFENAFKRVTL
                     EELGVQISINEAKFLGAYEHFYSDNFSGTNFSTHYIVLGYEINTMSHQINYPTLQHST
                     YNWFDIAELLADSSVHQYTKNYFK"
     gene            394..>1594
                     /locus_tag="RPM73_004472"
     CDS             394..>1594
                     /locus_tag="RPM73_004472"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDX6522721.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLTSRLTMLQETLRRL
                     EGVEHRPALVICNEVHRFIVAEQMRNEHLANSGILLEPKGRNTAPAVALAALKAVSSG
                     EDPILLVLAADHDIQDQERFISSILAAKEFAEEGKLVTFGIVPTKPETGYGYIKTGEN
                     LNEYGFKVSAFVEKPELDVAKKYLEDGGYLWNSGIFMFRASVFIDELNKFRPDILKIC
                     QQALKSSTQDLDFIRIDNDSFSCCPEESIDYAVMEKTTEAVVVPLNAHWSDVGSWSAL
                     WEISSKDKSGNAIRGDVLIHDSSDSYLYSQHRLIGVVGVKDLVVVETKDAILVAHKDK
                     VQKVKNIVEQLKVNNRAEYQQHREIFRPWGSHDSIAEGSRFQVKHVVIHPGHKTAKQI
                     HYHRTEHW"
BASE COUNT          503 a          265 c          339 g          487 t
ORIGIN      
        1 tataagaaac gaagaaggta aggcgctgct tggccagcgc ctcaatcgtc cagcacaaaa
       61 ttattggttt gtgccggggg ggcgaattct taaagatgag tctttcgaga acgcattcaa
      121 aagagttaca cttgaagaac taggtgttca aattagtatt aatgaagcaa aatttcttgg
      181 tgcatatgaa catttctaca gcgacaactt ttcaggaact aatttttcaa cgcattatat
      241 agtacttggg tatgagatta atacgatgtc acatcaaatt aactatccaa cactgcaaca
      301 tagcacttac aattggttcg atatagccga attgttagct gattcttcag ttcatcagta
      361 cacaaaaaat tattttaagt gaaggttatt tatatgctac ttcctgttgt aatggccggt
      421 ggttctggaa ctagattgtg gccactttct cgaacactgt atccgaaaca attcctatcg
      481 ttaactagtc gtttgactat gctacaagag accttaagaa ggcttgaggg ggttgaacat
      541 cgtcctgctc tggttatttg taatgaagtt catcgtttta tcgttgcaga acagatgcgt
      601 aatgaacatt tagcgaatag tggcatttta cttgagccga aaggtcgtaa tacagctcct
      661 gcagttgctc ttgctgctct taaagcagtt tcatcaggcg aggatcctat actgcttgta
      721 ttagcagccg atcatgatat acaagaccaa gagcgcttta tatcgtcgat attggctgca
      781 aaagaatttg cagaggaagg taagctggtt acttttggta ttgtgccaac caaacccgaa
      841 actggatatg gctacataaa aaccggagag aatttaaatg aatatggctt taaagtttct
      901 gcttttgttg aaaaacctga gctagatgtg gccaaaaaat atctcgaaga tggaggttat
      961 ctttggaata gtggaatatt catgttcagg gcctctgtgt ttattgatga gttaaataaa
     1021 tttagaccag acatactaaa aatatgccaa caagcattga agtcctctac acaagatctt
     1081 gattttatcc gtatagataa tgactcattt agttgttgcc ctgaagaatc tattgattat
     1141 gctgttatgg agaaaacaac agaggctgtg gtagttccat taaatgccca ctggagcgat
     1201 gttggttctt ggtctgcatt atgggaaatt agcagcaaag ataaaagtgg aaatgcaatt
     1261 agaggagatg tattaattca tgattcttcg gatagttatc tttactcaca acatagatta
     1321 attggtgtag taggagttaa agatttagta gttgttgaaa ctaaagatgc tattctcgtt
     1381 gcgcataaag ataaagttca gaaggtaaaa aatattgttg aacaacttaa agttaataac
     1441 cgtgcggaat atcagcagca tcgtgaaata tttagaccat ggggcagtca tgactctata
     1501 gcagaagggt ctcgatttca ggttaaacat gttgttattc atcctggaca taaaactgct
     1561 aaacagatcc attaccatcg tactgaacat tgga
//
LOCUS       DAPPNJ010000363         1545 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000363 DAPPNJ010000000
VERSION     DAPPNJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.365"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            17..376
                     /locus_tag="RPM73_004473"
                     /pseudo
     CDS             17..376
                     /locus_tag="RPM73_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            542..1510
                     /locus_tag="RPM73_004474"
     CDS             542..1510
                     /locus_tag="RPM73_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDX6522722.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
BASE COUNT          412 a          379 c          397 g          357 t
ORIGIN      
        1 acaggtgcag tgatttatgg ctgactcaac gagaaatcgc tcagcggagc gtttgattga
       61 tatccttgtt gaattacaga attatggtgt ggtcagtcgc cataatttga tgaaaaaata
      121 caacaacact gagcgaacag catatcgtga tctgaacatg ctttctccct ttattgaagc
      181 ctgtggcgat ggaaaatacc ggcttatttc agcaagagca ggaaaccaga gcaaagaatc
      241 cctgcacaaa tctctggcca ggttactgga tactgatgcg atttttcctg aacaggatga
      301 aggtttctgg cagaaattag aaaacagggc aaccgaaaag catattcgtg ttcagtttca
      361 taaccctgag cacactatgg aaggtgcgaa taagcaggtc atttcttccc aagctgactc
      421 gctgattaaa atttcgcgga tctgggccga tttttttccc gcaaacacat cgaatcagcc
      481 tatttagacc gtttcttcgc catttaaggg gctttgttga ataaatcgaa cttttgctga
      541 gttgaaggat cagatcacgc atcctcccga caacacagac cattccgtgg caaagcaaaa
      601 gttcagaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
      661 tttctggctg gatgatgagg cgattcaggc ctggtatgag tcggcaacgc cttcatcacg
      721 gggaagacct cagcgctatt ctgatctcgc catcaccacc gttctggtga ttaaacgcgt
      781 attccggctg accctgcggg ctgcgcaggg ttttattgat tccatttttg ccctgatgaa
      841 cgttccgttg cgctgcccgg attacaccag tgtcagtaag cgggcaaagt cggttaatgt
      901 cagtttcaaa acgtccaccc ggggtgaaat cgcacacctg gtgattgatt ccaccgggct
      961 gaaggtcttt ggtgaaggcg aatggaaagt cagaaagcac ggcaaagagc gccgtcgtat
     1021 ctggcgaaag ttgcatcttg ctgttgacag caacacacat gaagttgtct gtgcagacct
     1081 gtcgctgaat aacgtcacgg actcagaagc cttcccgggc cttatccggc agactcacag
     1141 aaaaatcagg gcagccgcgg cagacggggc ttacgatacc cggctctgtc acgatgaact
     1201 gcgccgcaaa aaaatcagcg cgcttattcc tccccgaaaa ggtgcgggtt actggcccgg
     1261 tgaatatgca gaccgtaacc gtgccgttgc taatcagcga atgaccggga gtaatgcgcg
     1321 gtggaaatgg acaacagatt acaaccgtcg ctcgatagcg gaaacggcga tgtaccgggt
     1381 aaaacagctg ttcgggggtt cactgacgct gcgtgactac gatggtcagg ttgcggaggc
     1441 tatggccctg gtacgagcgc tgaacaaaat gacgaaagca ggtatgcctg aaagcgtgcg
     1501 tattgcctga aaacacaacc cgctacgggg gagacttacc cgaaa
//
LOCUS       DAPPNJ010000364         1527 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000364 DAPPNJ010000000
VERSION     DAPPNJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.366"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(423..1514)
                     /gene="ychF"
                     /locus_tag="RPM73_004475"
     CDS             complement(423..1514)
                     /gene="ychF"
                     /locus_tag="RPM73_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDX6522723.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT          372 a          363 c          348 g          444 t
ORIGIN      
        1 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
       61 aacatcgata ttcatttttg gtcaatgggt tttctggcct atcgttgtta ttgtgctact
      121 ggttatcatt ttcatcttgc cagcatattg gagcgtgatc aattttgatc agctgtgaag
      181 gtggctgatg tgtagtgata caaaaactta cgttatatca ctgctaatga gatatccgtc
      241 atctgaacac ttatgtcttt aattattctc gtggttcact ataggcaata agcacaaaag
      301 tgtaggatgt tacaagaatg attaggactc ggtgaaatga aaaatccacg caattgcgtg
      361 gattttatat acttttgcgc tcttcatgag atttagcgaa acctcatgag acaataaatt
      421 aattagacgt tgaaaaggaa gttcatcaca tcgccatctt tcacgatgta atctttacct
      481 tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt gatgaaatct
      541 tcaaacgaga tggtttgtgc acggataaag cctttttcaa aatcagtatg gattttgccc
      601 gctgcctgcg gcgcggttgc tccaaccgga atggtccatg cacgcacttc tttcacccca
      661 gcggtgaagt aagtttgcag gttcagcagc ttataaccgg cacggatcac acggttcagg
      721 cccggctctt ccagcccaag ctcctgcata aactcgtcac gttcttcgtc gtccagttcg
      781 gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa cagaaccttc tttcgccgcg
      841 atttcacgca cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt gacgttggcg
      901 atgtacattg ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc tttctcttca
      961 gcgcttaaat ccagcgcgcg cagcatacct gcattttcca actggggcag gcatttttcc
     1021 aggaccgcca gctcagcttt cgcgtcttta tcgccacctt tggctttctt ctgtacgcga
     1081 tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt gataacttca
     1141 atatcgtcag ccgggttaac tttgccggaa acgtgaatga tgttgtcatt ttcaaagcag
     1201 cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg tcaggaactg gttacccaga
     1261 ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc catggtcgtg
     1321 ggaagcgtac gttgcggttt tacgatttca gccagttgat ccaggcgagg gtcaggcatt
     1381 ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc ttcaataccg
     1441 gctttggtca gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc gacgataccg
     1501 catttgaatc ccatgattta aatcacc
//
LOCUS       DAPPNJ010000365         1485 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000365 DAPPNJ010000000
VERSION     DAPPNJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.367"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..195)
                     /locus_tag="RPM73_004476"
     CDS             complement(<1..195)
                     /locus_tag="RPM73_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDX6522724.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRHLSEFHDVELVVKTHKELDLTNQ
                     QDVETFFEQEKIDEVYLAAAK"
     gene            complement(199..1317)
                     /gene="gmd"
                     /locus_tag="RPM73_004477"
     CDS             complement(199..1317)
                     /gene="gmd"
                     /locus_tag="RPM73_004477"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDX6522725.1"
                     /translation="MKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTDRVD
                     HIYQDPHISNPKFHLHYGDLTDTSNLTRILSEVRPDEVYNLGAMSHVAVSFECPEYTA
                     DVDAIGTLRLLESIRFLGLENKTRFYQASTSELYGLVQEVPQKETTPFYPRSPYAVAK
                     LYAYWITVNYRESYGMFACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NLDSLRDWGHAKDYVRMQWMMLQQDKPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GKGVNEIATVVHKTSDCAVGVNIGDVIVRVDPRYFRPAEVDTLLGDPTKAKEVLGWEP
                     EITIEKMCSEMVASDLEKAKQHAFLKSHGYDVAVSLER"
BASE COUNT          468 a          321 c          228 g          468 t
ORIGIN      
        1 ttttgcagcg gcaagatata cttcatctat tttctcttgt tcaaaaaaag tctcgacatc
       61 ttgttgatta gtcaaatcga gttctttatg agtttttaca actaactcga catcatgaaa
      121 ttctgataag tgtcggcata tagcagaacc aaccatacca cgatgccccg caacataaat
      181 acgcttcttt gtcattactc aacgctccag agatactgct acgtcataac catggctttt
      241 aagaaatgca tgctgttttg ctttttctaa atcactagca accatttctg aacacatctt
      301 ttcaatagta atttctggtt cccatcctaa cacttctttc gctttagttg gatcccccaa
      361 cagcgtatca acctctgcgg gacggaaata gcgagggtct acgcgaacaa taacatctcc
      421 aatgtttacc ccaacagcac agtcagatgt tttatgtaca accgtcgcaa tttcattaac
      481 acctttacct gaaaattcaa gttccagccc aacttcttta gcggacatac gaacaaattc
      541 acgcacagat atttgtttac ctgttgctat aacgaaatcc tctggtttgt cttgttggag
      601 catcatccat tgcatacgaa cataatcttt agcatgtccc caatcacgaa gcgaatccag
      661 attaccgaga tataggcatt tttcaatacc ttgagaaatg ttagctatag cacgtgtaat
      721 tttacgcgtt acgaatgttt ctccacggcg tggggattca tgattgaaca aaatgccatt
      781 acaagcaaac ataccatatg attcacgata gttaacagtt atccaatagg cataaagctt
      841 ggcaactgca tatggtgatc ttggataaaa aggcgttgtt tctttttgtg gaacttcttg
      901 aaccaatcca tacaactcag atgttgatgc ttgataaaat cttgtcttat tttctaatcc
      961 taaaaaacga atagactcca acaatcttaa cgttccaatt gcatcaacat cagcggtata
     1021 ttctggacat tcaaaggaaa ctgcaacatg actcatagct cctaaattat atacttcatc
     1081 tggcctaact tctgaaagta tacgtgttaa gttagaagta tcagttagat caccataatg
     1141 aagatggaat tttggattac taatatgtgg atcctgataa atatgatcaa ctcgatcagt
     1201 gttgaacgaa gatgctcgac gtttgatacc gtgaacttcg tatccctttt ctaataaaaa
     1261 ttcagccaaa taagaaccgt cttgtccagt aatacccgta atcagagcta ctttcatttt
     1321 aaatatctca cctcatcata aaaactaaca gtatcagaaa agcatttatc ccaagtgaat
     1381 ttcttggcat aatgtagacc attatatatc attttctctc taaaactttc atctaataat
     1441 aaattaatac acttagaaaa ttgttcaaca gttgcctctt tggct
//
LOCUS       DAPPNJ010000366         1481 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000366 DAPPNJ010000000
VERSION     DAPPNJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.368"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(106..759)
                     /gene="ribB"
                     /locus_tag="RPM73_004478"
     CDS             complement(106..759)
                     /gene="ribB"
                     /locus_tag="RPM73_004478"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDX6522726.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(863..1011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1133..1423
                     /gene="ubiK"
                     /locus_tag="RPM73_004479"
     CDS             1133..1423
                     /gene="ubiK"
                     /locus_tag="RPM73_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDX6522727.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          411 a          388 c          342 g          340 t
ORIGIN      
        1 tagaaagaga agcggaatta acatcttgca tagcggggca aaataaaaac cgaagccagc
       61 tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg
      121 ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt
      181 attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc
      241 acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt
      301 atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg
      361 gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg
      421 atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc
      481 ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg
      541 atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg
      601 ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc
      661 atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc
      721 gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa
      781 ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga
      841 gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc
      901 ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa
      961 ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact
     1021 ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta
     1081 cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga
     1141 cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag
     1201 ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg
     1261 cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg
     1321 tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca gtactgagat
     1381 taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc
     1441 actgtaaatt atgaattaat cctctaattt acagtgcttt c
//
LOCUS       DAPPNJ010000367         1466 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000367 DAPPNJ010000000
VERSION     DAPPNJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.369"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RPM73_004480"
     CDS             complement(<1..902)
                     /locus_tag="RPM73_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX6522728.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
     gene            complement(1213..1317)
                     /locus_tag="RPM73_004481"
     CDS             complement(1213..1317)
                     /locus_tag="RPM73_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDX6522729.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT          438 a          319 c          323 g          386 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaagtg cctagctgaa taacggcgag gcggaaagga
       61 cgagcctctt ttgcccgctg cggtgcaact tctgtaatca gctcacgcag gtaactttct
      121 tcaaaacagt gagcgtcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg tcgcgccagc ctgtagcaac gcaccgtggt ggttagattt atggttattg
      241 cggtcaaaca acaccagatc gcccggcgtc agcaatgcgt ttaagaccac tttattagaa
      301 gatgaagtac cgtttaaaac gaaataggtt ttatcggcat taaagacttt agccgcatgc
      361 tgttgtgcaa tacatggcgc accttcatga atcagcagat cgcccattgc tacgtcagca
      421 ttacacaagt cagagcggaa gagcatttcg ccaaagtatt ccacgaactg attgcctgcc
      481 ggatggcgac ggaaaaactc acccccctga tgaccagggc agtcaaaagc actattgccc
      541 tgattaacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcgt ttcataatgg
      601 ctggcagcgg tttctaactg acgtccataa aactctttac gtgattcgca atgttcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgtcatagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ctgactgata aaataatggc ggcaacgtca atataattac tttcgtctat attcacgcat
      841 gcacgatgag tggaaaaaca atcagggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcacgccg gaaaaaaagc aaagctccac cgctgataaa taaatcagta gtttgctttt
     1021 tgcagatgca ataacccaga ccagcccggt aactggctat actcgtcata cctcaagttg
     1081 caagtgctgc gactcgaatt atttagagtt taaaagggtt agcagtatat gagcaccgag
     1141 gaatacaggt attaacctgt aatgcttaaa gtcagggaga cctgtacgtt ggggtgccct
     1201 ttagtcagaa gactagcggg cattaaagaa atgaaagtca aaactattac gatgggcaaa
     1261 cttcatgata tgggttgtcc gccttatatg aggcattaag cggttattat tttccattta
     1321 cctttacctc ttgctgtgtc agttgaaggt agagttattc tatccatgaa atggctataa
     1381 tctgtgaaga agatcaacaa aaaacgatca taccccaaat aataaatcat taattatgaa
     1441 tagcgccata ttagatgctg ttttgt
//
LOCUS       DAPPNJ010000368         1456 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000368 DAPPNJ010000000
VERSION     DAPPNJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.370"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            809..1411
                     /gene="fimB"
                     /locus_tag="RPM73_004482"
     CDS             809..1411
                     /gene="fimB"
                     /locus_tag="RPM73_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDX6522730.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          427 a          257 c          326 g          446 t
ORIGIN      
        1 cagtgagcgt ggtgaaaatc agtgtttacc cgccatcagg ctgagcataa ttctcatcat
       61 gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt ctgaagtcga tctggagagg
      121 cttgttgatg ttggtgtttt caggatgatg tttcacttag tttgtttgcc gtatcgcccg
      181 gcgaatggct gtgattgagg aaggtttaag tcgtagtgac caaagctata tttaccaacg
      241 aatgtagatg aaaaaatcat ctcctgcgtt ccccatatct ccaggataaa aaggaatgta
      301 acaatctcat ggcgtaagct gacgaatcag ccaggaataa tcgctaggga cctaagaatt
      361 agcatgataa tagccactaa gaaattactg cgctccatga aatagccatt ttgtggcaaa
      421 tggagttgac taataatgtc atatgtgaga cggctagttg aacgaatatt aaattttgct
      481 gaatttttta tgttgatttt acttgttaca gaacatatca catgatatat agataagatt
      541 agttgcatta atgatgaggg ttattattag attcgtatcc gattgataaa tatataaagg
      601 tacatagcat gcaagagcat ggcgtttgta tggcaacgtt attataatta actgttgcta
      661 ctccatttaa gttcactcag aagaactggt ccacttacgt tagttattaa gcaaacgttc
      721 gcttttataa acataatcag gataaaaatg ttggattatt gctaacccag cacagctagt
      781 gcgcgtctgt aattataagg gaaaaacgat gaagaataag gctgataaca aaaaaaggaa
      841 cttcctgacc catagtgaaa tcgaatcact ccttaaagca gcaaataccg ggcctcatgc
      901 agcacgtaat tattgtctga ctttgctttg ttttattcat ggtttccggg cgagtgaaat
      961 ttgtcgattg aggatttcgg atattgatct taaggcaaag tgtatatata tccatcgatt
     1021 aaaaaaaggc ttttcaacaa cgcatccgct attgaacaaa gaagttcagg ctttaaaaaa
     1081 ctggttgagt atccgtactt cgtacccgca tgctgagagc gagtgggtat ttttatctcg
     1141 taaggggaat ccgctttctc ggcaacagtt ttaccatatt atctcgactt ccggtggtaa
     1201 tgccgggttg tcactggaga ttcatccgca catgttacgc cattcgtgtg gttttgcttt
     1261 ggcgaatatg ggaatagata cgcgacttat ccaggattat cttgggcatc gcaatattcg
     1321 tcatactgtc tggtataccg ccagcaatgc agggcgtttt tacggcatct gggatagagc
     1381 cagaggacga cagcgtcacg ctgttttata gagatagttg agataccagg gatggtgtta
     1441 aaaactacat tattct
//
LOCUS       DAPPNJ010000369         1444 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000369 DAPPNJ010000000
VERSION     DAPPNJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.371"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            181..1392
                     /gene="bluF"
                     /locus_tag="RPM73_004483"
     CDS             181..1392
                     /gene="bluF"
                     /locus_tag="RPM73_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDX6522731.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          420 a          302 c          300 g          422 t
ORIGIN      
        1 taaaacatat tgcatcattt aactgacaat ctatacaaga tttttcgcca actagtgttc
       61 aactttttcc attttttatc aatgtaaaga aacggttatg ttttggtatt aaaagaaaga
      121 cacaagaaga tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta
      181 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
      241 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      301 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      361 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      421 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      481 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      541 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      601 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      661 cttgattcct gtgccctttc accaactata aacgaccact ttgcctttca tcctattgtc
      721 gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagatagc
      781 ccatcagcca tagcggttgg gcagcgtaaa gacggggaaa tctacacagc ggatctcaaa
      841 agtaaggcgc ttgcattcac gatggcacac gcacttgagc tcggtgataa aatgatttca
      901 atcaatctat tacctatgac cctggttaac gaacctgacg cagtctcttt tttacttaat
      961 gaaataaagg ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa
     1021 gtcatatctc ggtttgatga gtttgccgaa gcgattaaat cgctaaaggc tgctggtatc
     1081 agtgtagcaa ttgatcattt tggcgcaggt tttgctggtt tgttactcct gtcacgcttc
     1141 cagcctgaca gaattaaaat cagtcaggaa ttgattacca atgttcataa aagtgggcca
     1201 cggcaggcaa ttattcaggc gatcataaaa tgctgtacat cacttgaaat tcaagtcagt
     1261 gctatgggcg tggcaacacc agaagagtgg atgtggcttg aatccgcagg aattgagatg
     1321 tttcagggag atctgtttgc taaagctaaa ttgaatggta tcccttccat agcgtggcca
     1381 gagaaaaaat aattttcagc acattctttc acatgatttc agtaaatcat cgtgaaaatg
     1441 aata
//
LOCUS       DAPPNJ010000370         1391 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000370 DAPPNJ010000000
VERSION     DAPPNJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.372"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            6..446
                     /locus_tag="RPM73_004484"
     CDS             6..446
                     /locus_tag="RPM73_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDX6522732.1"
                     /translation="MSKKKFSVENEDLSTVRTSLLNSNLVRKLIMEMSDEDFNQVKDN
                     LEKAIEQRNELKDSLAIIKKYMDDAGVTAEDVYREFGKKLQTGRKNDSGESNKYRYEY
                     NGQIMYWSGQGRRPSVIAEAIKAGGSLDDFLISQGTSSSCSDYE"
     gene            715..843
                     /locus_tag="RPM73_004485"
                     /pseudo
     CDS             715..843
                     /locus_tag="RPM73_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(873..1222)
                     /locus_tag="RPM73_004486"
                     /pseudo
     CDS             complement(873..1222)
                     /locus_tag="RPM73_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          386 a          279 c          331 g          395 t
ORIGIN      
        1 gatagatgag caagaagaag ttttctgttg agaatgaaga tctctctact gttcgtacat
       61 cacttcttaa ttccaacctt gtccggaagt tgatcatgga aatgtctgat gaagacttca
      121 atcaagttaa ggataacctt gaaaaggcca ttgagcagcg taatgagttg aaggattctt
      181 tggcaattat caaaaagtac atggatgacg caggtgtaac ggcggaagat gtgtatcgtg
      241 aatttggcaa aaaattacag acgggcagaa aaaacgattc aggagagtct aataagtaca
      301 ggtatgagta taacggtcaa ataatgtact ggagtggaca aggtcgtcgg ccttctgtta
      361 ttgcagaagc aataaaagcc ggaggttctc ttgatgattt tcttatatct cagggcacat
      421 catcatcttg ttctgattat gagtgataca tctaatcggt tgaatgttgc tgtaatgctc
      481 gcttttgatg cttatatggc agcgttaccg tttattgttt tagttacaaa ttggtcgtca
      541 tggacataac ctgaaattat aactatcatc gggtctttca agttgcttat gcatataacg
      601 ccagggagga aaacgagagt taaaaaattg aactgttctc atagagactg taatttaaat
      661 tgtgtaattg ccggtttttg atatgttcac tccaacaacg tagacaagta gattgtggac
      721 gaaaagacgc ttaaagtcct tgcggctgaa ctggctaaag gcctcaaaac cggggccgat
      781 ctcaatcagt tttcccgtat gctgacgaaa ttaactgtcg aaacggcgct taatgcggaa
      841 ctcggtaacg gctgcagatg ttcctgctct ggtgtatgct cagcatcctg ttttggtgcg
      901 gcgaagacat ggaagcggcg ggagaacccc agcgtattaa ccgcaaagtt aactttgcac
      961 cgttgcccgg caacctccac ctcaacttcg ccctagtcgt gctggagctg gtatccaggc
     1021 tgagtttcga agcgaactgt tctttttgac ggacgcattt tacgtttggg ctggatgtag
     1081 taacgcagca tggaacgtcc gcccgtataa cccattgcct gaccgttcgt tcagagcaac
     1141 caatctgagt cgcaatatcg acaatgtacg cgccctgctg gcgcatttgc tttatcatgt
     1201 aaaagtcctc tctgctcagc atgttgatgt catttctggt gtgagaacct taaggaaaca
     1261 acatgttggg tggagcggaa aatccaaatg gtgaccgtct tattatcgct ggcgctgaca
     1321 cctgagcgat cacctttaat atggtagcaa ttacactgaa ttacttacag attcgttata
     1381 acgaacctct g
//
LOCUS       DAPPNJ010000371         1385 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000371 DAPPNJ010000000
VERSION     DAPPNJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.373"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(53..931)
                     /gene="glxR"
                     /locus_tag="RPM73_004487"
     CDS             complement(53..931)
                     /gene="glxR"
                     /locus_tag="RPM73_004487"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDX6522733.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1031..>1385)
                     /locus_tag="RPM73_004488"
     CDS             complement(1031..>1385)
                     /locus_tag="RPM73_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDX6522734.1"
                     /translation="IEPINHFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQRM
                     EGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKP
                     QTTTEAGLRWMDPYR"
BASE COUNT          357 a          361 c          300 g          367 t
ORIGIN      
        1 taatattgct ggccgattca ttttctgata tcggccattt ttattgcggg tatcaggcca
       61 gtttatggtt agccattaat tccagcgcct gcactaacgc agagtgatcc aactggctgc
      121 caccgtttgc cgcacaggta ttaaataact cctggcaggt cgcagtgttt ggcaggttca
      181 gcgcaagtgc tttcgcactt tgcagtgcca ggttgagatc tttctggtgc agagcgattt
      241 tgaagcccgg attaaaggtg cgtttaatca tacgctcgcc atgaacttcc agaatacgtg
      301 aggaagcaaa gccgcccatc agcgcctggc gcacacgtac cgggtccgca ccggcttttg
      361 aagcaaatag cagggcttca gaaaccgctt caatattgag cgccacaata atctgatttg
      421 ccactttgca ggtttgacca tcgccgttac cgcccacgag ggtgatattt ttaccgagca
      481 gttcaaacag cggtttaaca cgttcaaata ccgcttcatc accgccaacc ataatcgaca
      541 acgtcccttc acgcgcaccg atttcaccgc cggagactgg cgcatcgaga taatcgccgc
      601 ccagttcatt cacctgacga gcgaaacgct tagtttcaat cggggaaatg gagctcatat
      661 caacaatggt tttgcccttc agcgaggctt tggtacaacc attttcaccg aacagaactt
      721 cttcaacctg tggtgtatct ggcaccataa taaaaatgat gtccgatgct tccgttacct
      781 ggcgcgcagt ttcaacactg acggcaccca gtgacagtaa ttcatcagca accggtccaa
      841 tggttgtgac atgcagttga tgaccggcac gcgccagatt aatggccatc ggtgtaccca
      901 taatgcctaa gccaataaat cccagtttca taattaacct cttttaaatt tcgcttttcc
      961 tgaattcaga caacacgatc ccctgccaac ttagcggcct aaaaaagcat tgcctgaaca
     1021 gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg gtggtttggg
     1081 gtttatattc acacccaacc caaccgttgt agtcagaatt ttcgattacc ttaaagagat
     1141 aatcataatt aatttctccg gttcccggtt cgccgcgatg cggattatcg gcaatttgca
     1201 ggtgaccaat tttatcagcc cactgagtca tggtgttggt taattcacct tccatccgct
     1261 gcatgtgata aatgtcatac tgaattttca aattgcagca accaacatca tcaatcaatt
     1321 tcagcgcctg ccgagttccg gtgagatgga aaccaggaat atcaaaatgg ttaataggtt
     1381 caatc
//
LOCUS       DAPPNJ010000372         1339 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000372 DAPPNJ010000000
VERSION     DAPPNJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.374"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..338)
                     /locus_tag="RPM73_004489"
     CDS             complement(<1..338)
                     /locus_tag="RPM73_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDX6522735.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKR"
     gene            complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RPM73_004490"
     CDS             complement(559..1290)
                     /gene="bluR"
                     /locus_tag="RPM73_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDX6522736.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYTAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          341 a          312 c          302 g          384 t
ORIGIN      
        1 ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgcc tgctagtgtt
       61 gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc ctcttttatt
      121 ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct taataacaaa
      181 accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      241 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      301 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      361 ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      421 ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg agcattttgc
      481 aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt ctgtctaagg
      541 ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt gctcacccca
      601 gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
      661 ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa cagaaatgtg
      721 ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct cttcaacatt
      781 ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa gcgaggctgc
      841 tgtgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg tgttgtgatc
      901 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
      961 ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca tgcgaagaat
     1021 tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc tggtggtttc
     1081 cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct tgatctcttc
     1141 gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gctgtggttt
     1201 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1261 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca aatctccgtc
     1321 gcctgtacac gacccaata
//
LOCUS       DAPPNJ010000373         1337 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000373 DAPPNJ010000000
VERSION     DAPPNJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.375"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>1337
                     /gene="rhsB"
                     /locus_tag="RPM73_004491"
     CDS             <1..>1337
                     /gene="rhsB"
                     /locus_tag="RPM73_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDX6522737.1"
                     /translation="TPDGRASAFYYNHHNQLTSATGPDGLELRREYDESGRLIQETAP
                     DGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQ
                     VTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQY
                     DAWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQ
                     RYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHIS
                     EGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPA
                     VEWLAYGSGYLAGMKLGDTPLVDFTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLN
                     SLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYAT"
BASE COUNT          320 a          419 c          389 g          209 t
ORIGIN      
        1 acgccggatg gcagggcatc ggcgttttac tataaccacc acaaccagtt aacgtcagcc
       61 accgggcctg acgggctgga attgcgccgg gaatatgatg aatccggccg tctgattcag
      121 gaaactgccc ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta
      181 ccctgcgcaa cggaagatgc caccggcagc cggaaaacca tgacgtggag ccgttacggt
      241 cagttgctga ccttcactga ctgttccggt tatgtaaccc gctatgacca tgaccgtttt
      301 ggtcaggtga cggcggtgca ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc
      361 cgtggacagt taactgcggt gaaagacacg cagggccatg aaacgcggta tgaatacaac
      421 atcgccggtg acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac
      481 gatgcgtggg gaaaggccat ccgtaccacg cagggcggtc tgacgcgcag tatggaatac
      541 gatgctgccg gacgggtcat ccgcctgacc agtgaaaacg gcagccacac caccttccgt
      601 tacgatgtac tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat
      661 caccacgacc tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg
      721 cactatgacg aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg
      781 tggcggtacg acgaacgcgg ctggctgaca gacatcagcc atatcagcga agggcaccgg
      841 gtgacggtgc attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg
      901 catcacccgc agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg
      961 cagggactgg cgaaccgctg tataccggac agcctgcccg ccgtggaatg gctggcctac
     1021 ggcagcggtt acctggcagg catgaagctc ggcgacacac cgctggtgga tttcacccgc
     1081 gaccgcctgc accgggaaac gctgcgcagc ttcggccgtt atgaactcac caccgcttat
     1141 acccctgccg ggcagttaca gagccagcac ctgaacagcc tgcagtatga ccgcgattac
     1201 acctggaacg acaacggcga actcatccgc atcagcagcc cgcgccagac ccggagttac
     1261 agctacagca ccaccggcag gctgaccggc gttcacacca ccgcagcgaa ccttgatatc
     1321 cgcatcccgt atgccac
//
LOCUS       DAPPNJ010000374         1332 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000374 DAPPNJ010000000
VERSION     DAPPNJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.376"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..1330)
                     /locus_tag="RPM73_004492"
     CDS             complement(<1..1330)
                     /locus_tag="RPM73_004492"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDX6522738.1"
                     /translation="MSTCSLKFNSEECSAFIKVLDFTVINATLITSAWFFETKYFGTA
                     VLFCLLFSTLFLLFSEYTKVYQRKIRKFRFRDFKGILGSALLAILLCEVIRYTLAQVY
                     PQGDFNSLGDDFVSPIVVWYLLPFPALLCIRYLLLKYTSRKSTRVAIVGVTENGLAVE
                     EALRNEYSNMQLDLAFYDERDFSRLDDVAKKIKSPFKGSVQTLVEEARRGNVDEIYIA
                     LPMVALQRIRHFLSMMSDTTVDTYIVPDFYTYSNNMSKFRNIHDLHTIAIFSSPFEGV
                     SSFIKRAEDLIVGSIIMVMISLLMLIIAIGIKLTSRGPVFFKQDRYGLSGQKIKVWKF
                     RTMKVMENDDVVKQATKNDPRVTPFGAFLRRTSLDELPQFINVIQGSMSIVGPRPHAV
                     AHNELYRKQVENYMIRHKVKPGITGLAQVNGFRGEIDTLYKMEKRVQYDIE"
BASE COUNT          412 a          304 c          261 g          355 t
ORIGIN      
        1 attcaatgtc gtactgaact cttttttcca ttttatagag cgtatcaatt tccccacgga
       61 agccgttgac ctgcgcaaga ccagtgatcc ccggttttac cttatgacgt atcatgtagt
      121 tctcaacctg cttacggtac agctcgttat gtgcaaccgc atggggtcgg gggccaacaa
      181 ttgacatact tccctgaatg acgttgataa attgcggcag ctcatcgaga gaagtgcggc
      241 gtaaaaaggc cccgaagggt gtcacccgag gatcgttctt cgtggcttgt ttcacgacat
      301 catcgttttc catgaccttc atggtgcgga atttccacac tttaatcttt tgtccgctta
      361 gcccgtaccg atcctgttta aaaaatacag gtccacgaga agtcagtttg atgccaatcg
      421 caataatcag cattaataag gaaatcataa ccatgataat gctgcccaca atcagatctt
      481 ccgcacgctt gataaatgaa ctcactcctt cgaacggcga actgaaaata gcaatggtat
      541 gcagatcatg gatattgcga aatttggaca tattgttact gtaagtataa aagtcaggaa
      601 caatataggt atccaccgta gtatctgaca tcatcgacag aaaatgacgg atacgctgca
      661 aagcaaccat cggcagagcg atataaattt catcaacatt ccctcgtctg gcctcttcga
      721 ccagtgtctg taccgaaccc ttaaatgggc ttttaatctt ctttgctaca tcatcgagcc
      781 gcgaaaaatc ccgctcatca taaaaagcaa ggtccagctg catgtttgag tattcgtttc
      841 gcaatgcctc ttcaactgcc aggccgtttt cggtaacacc aacaattgcc acccgggtgc
      901 tttttctgga cgtatactta agaagtaaat agcgaataca caaaagagca ggaaacggta
      961 atagatacca tacgacaata ggggaaacaa aatcatcacc cagcgagtta aaatccccct
     1021 gaggatatac ttgtgcaaga gtatacctga ttacttcaca aagaagtata gccagtaatg
     1081 cactgccgag aatcccttta aaatcacgaa aacgaaattt cctgattttc cgttggtata
     1141 cctttgtgta ttctgaaaat aacaaaaaga gagtagagaa gagcagacaa aataatactg
     1201 ccgtaccaaa atacttcgtt tcaaagaacc aggcgcttgt aatcaatgtt gcattgatta
     1261 cagtgaaatc aagcaccttg atgaatgcag aacattcttc actattgaat tttaatgaac
     1321 atgtcgacat at
//
LOCUS       DAPPNJ010000375         1313 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000375 DAPPNJ010000000
VERSION     DAPPNJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.377"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(22..1188)
                     /gene="ugd"
                     /locus_tag="RPM73_004493"
     CDS             complement(22..1188)
                     /gene="ugd"
                     /locus_tag="RPM73_004493"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDX6522739.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMIIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT          367 a          276 c          284 g          386 t
ORIGIN      
        1 aatcgccctg ataacaggat gttagtcgct gccaaaaaga tcgcgggtgt agactttgtc
       61 tgccacatcc ttaagctctt ctgccatacg gttggagata atgacgtcgg cttgttgttt
      121 gaaggtggcg agatcacgtt ccaggcgaga gttgaagaat gaatcttctt tcatcaccgg
      181 ttcgtagatg atcacttcaa cgcctttcgc cttgatacgt ttcataatcc cctgaatgga
      241 agacgcacgg aagttatctg acccgctctt cataatcaga cgataaatac ccaccacttg
      301 cggtttgcgt gacaaaatgg catcggcaat aaagtcttta cgtgtgcggt tagcatcgac
      361 aattgccgaa atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc
      421 tttcggcaga caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaattcg
      481 tgggtcgaga caaacgcctt cgattatttg acgggaattc agacctaaac tttctgcgta
      541 gctatccagt tcgttaaagt acgccacgcg catcgccagg taggtgtttg caaaaagttt
      601 aatcgcttct gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgctcc
      661 ttcctgtaat aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg
      721 tgaaggatgg agattatcgt aaagggcttt accctcacgg agaaattccg gagagaaaat
      781 aatattttca gtacgatatt tcttatgcat cgctgcggta aagccaacgg gtaccgttga
      841 tttgatgatc ataaccgcat aaggatttat ctctacaacg tctttaatta ctgattctac
      901 actcgatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg cgataatgac
      961 ataatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc
     1021 tgattgcaaa aactgctgaa tttccttatc aactatagga gatatccgat cattcagcat
     1081 agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag
     1141 cccgtttgac aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca
     1201 gaattaactt aactgtgaat catgatgttt ttagcatcct gataagagct aaaagtttta
     1261 acgctacgtt gttcgatggt caacgcaacg aaaaacaatt cagattttct tta
//
LOCUS       DAPPNJ010000376         1297 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000376 DAPPNJ010000000
VERSION     DAPPNJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.378"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..915
                     /locus_tag="RPM73_004494"
     CDS             <1..915
                     /locus_tag="RPM73_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDX6522740.1"
                     /translation="FSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIPFYWGDDI
                     AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNL
                     SWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGINITNQLSDTFAILDAPGLEGA
                     HINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSY
                     VQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIK
                     VALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            928..>1297
                     /locus_tag="RPM73_004495"
     CDS             928..>1297
                     /locus_tag="RPM73_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDX6522741.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPY"
BASE COUNT          420 a          253 c          246 g          378 t
ORIGIN      
        1 ttcagttact ccaataactg gcaacacatt agttatactt tctctgccag ccaatcttat
       61 gatgaaaata ataaagaaga ggagcgtttt aatctgttta tctccattcc tttctactgg
      121 ggggatgata ttgccaaaac acgtcaccaa attaacttat cgaattcgac ctcattttcc
      181 aaagatggct attcctccaa caatactgga attactggca tagccggtga acatgatcag
      241 ttaaattatg gtatatatgt taatcagcaa caacaaaata atgatacctc gcttggtacg
      301 aatttaagct ggagaactcc catcgccata atagatggca gctatagtca ttctaaaaac
      361 gcctggcaaa gtggtggaag tattagtagt ggattagttg tctggtccgg tggtattaat
      421 atcactaacc agttatccga tacatttgca attctggatg cgcctggatt agaaggtgca
      481 catattaatg gacaaaaata caatcgaaca aacagcaaag gccaggttgt ttacgacctg
      541 ataatacctc atcgtgaaaa ccatctggta cttgatatag caaacagtga aagtgaaaca
      601 gaattgcagg gcaatcgtca aattattgca ccttaccgtg gagcagtttc ttatgtgcag
      661 tttacaactg accaacgtaa gccctggtat atacaggcac tgcgtcccga tggttcgcca
      721 ttaacctttg gctatgacgt actggatctc caggaaaata atattggagt cgttggccag
      781 ggtagtcgcc tttttattcg cgtagatgaa attcccactg gaataaaagt tgctctcaat
      841 gatgaacaga acttattctg tactattact tttcaacacg ttatcgatga aaacaaaaca
      901 tatatatgcc agtaaaagga gtttactatg aagtggcttt tattaatcac cttgagtcta
      961 tattctttta ttgtgcaaag tgctccctgt gcattgacca atgtcgggga acaacgtgga
     1021 acatatatac tcaagccact gagtatgaaa ggtaatctca ccgccgaaaa aattgaaaac
     1081 accggaaata tcattgagtg tgcagactta caagagacga ccaacgccat taaagttcat
     1141 tttttaacac agaacgcttt aaggtttaaa gtcggaagtc gtcattatat tattaatttc
     1201 ccctttgaat ctgacggtaa aaaaatagag cttccaggaa ggtcatctta taatttagaa
     1261 gaaatgctat ctgcgataaa tttcaccatt ccatatt
//
LOCUS       DAPPNJ010000377         1268 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000377 DAPPNJ010000000
VERSION     DAPPNJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.379"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            157..660
                     /locus_tag="RPM73_004496"
     CDS             157..660
                     /locus_tag="RPM73_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDX6522742.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(739..990)
                     /locus_tag="RPM73_004497"
     CDS             complement(739..990)
                     /locus_tag="RPM73_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDX6522743.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="RPM73_004498"
     CDS             complement(1105..1191)
                     /gene="azuC"
                     /locus_tag="RPM73_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDX6522744.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          342 a          294 c          282 g          350 t
ORIGIN      
        1 atacccgttc agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca
       61 tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct
      121 tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc
      181 aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt
      241 aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag
      301 agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc
      361 atcgttaaag ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa
      421 aaaacgatgg aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg
      481 aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag
      541 catgatggtc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt
      601 atgtgccctg tgcagaccga ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga
      661 tcatcatcgg cgctaatgca ttgcgccgat gaaggttttg agaaaccgct gcctcatctg
      721 tttgaagcag cggttttttt aatgggattc accctgtggg gtaaacttga gttcaataag
      781 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt
      841 aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac
      901 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccaa
      961 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     1021 ttatagtagc gacttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     1081 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     1141 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     1201 ccgaccattt gttatacagg tgttattgtc tttgcgctta taatatgaca accatcacaa
     1261 aaatcaat
//
LOCUS       DAPPNJ010000378         1262 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000378 DAPPNJ010000000
VERSION     DAPPNJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.380"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..231
                     /locus_tag="RPM73_004499"
     CDS             <1..231
                     /locus_tag="RPM73_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX6522745.1"
                     /translation="DNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKI
                     DNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            493..>1262
                     /locus_tag="RPM73_004500"
     CDS             493..>1262
                     /locus_tag="RPM73_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX6522746.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDT"
BASE COUNT          422 a          213 c          246 g          381 t
ORIGIN      
        1 gacaattatt ttaataacac tactgctttc agaccaaaac ctgaagatat cttcaaatgt
       61 ggacagacgc tcaaaaaaac taaacagaaa tatggccatt atggattagg ggtagatcag
      121 catcaaaaaa ttgataatta taccatgggg ctaaattcct ggaagatagc tcctaacgaa
      181 cgtggtgata aaaaaggtgt cccagggctt agttgttttc aaccacatta aaactccatg
      241 agtgctgtgg tatatctttt taatcctgcc agtatttcat aggttttctt aatcgattgt
      301 agaggcatgt tctgatgacg cctctacaat ctttagggac tttctctgct atgagataag
      361 aaatttcata ttatgcttta aggctgaatg atttgtgcaa aatataatta tttttccatc
      421 gaacatgagg tgtatgtcat aagattaaat gttaactatg cattcctcgc ggtgaattat
      481 atgaggtaaa agatgaaaat tggaactgtg gcaggcacta acgacagtac aacgacaatc
      541 gcaacaaatg atatggtgca agagcatgtt actaacttca ctaaagaact gtttgggtat
      601 attgcaaatg gtataggtga tgatatatca agtattgcca ggacaatgct cggtgaagtt
      661 gtggaaaaaa ttgatgattg gcaaatagaa cgtttccaac aatctattcg agatgacaaa
      721 atatcattta ccatacaaac gaatcattca gaaaaatata gtatgctatc ggacatgcgc
      781 gctcatattc tacgtaggga taataattat cagtttattg tgacaattaa tagtaaaaat
      841 tacggctgct atcttgataa taccgatatt aattggtgca gcattgttta tttacttaat
      901 aatatgacag taaatgatga tgctaatgat gttgcagtta ctgagtcgta caaacccgtc
      961 tgggactgga aaatatcgca attcaatgta tctgatatta aattcgaaac aatgataaac
     1021 ccccagttcg ctgacagaac ctatttctcc aattgttcac ctgttgatcc cacaagtacc
     1081 agaccaacct attttggcga taccgatggc tcagtcggtg cagttttgta cgcgcttttt
     1141 gcaacgggac atttgagaat catggctgaa ggagaaaatt tccttagcca gttgcttaat
     1201 atagaagatg aagttctcaa cgtattgcta agggagaact ttaatgagca acttgacact
     1261 aa
//
LOCUS       DAPPNJ010000379         1256 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000379 DAPPNJ010000000
VERSION     DAPPNJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.381"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            130..807
                     /locus_tag="RPM73_004501"
     CDS             130..807
                     /locus_tag="RPM73_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDX6522747.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            879..1163
                     /gene="ppnP"
                     /locus_tag="RPM73_004502"
     CDS             879..1163
                     /gene="ppnP"
                     /locus_tag="RPM73_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDX6522748.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          312 a          276 c          315 g          353 t
ORIGIN      
        1 aatctttaca ttatgccgtg cacgtctgct gctacgcttt ttgtcatttg tagcacaagt
       61 aagtgtcagc agtggtgctt cacacttgcc cggtaattaa cgacgaaaga aaagtaaggt
      121 ggatgaacaa tgagtgcgtc gttggcgatc ctgaccatcg gcattgtacc tatgcaggag
      181 gttttgccgc tcctgacgga atacattgac gaagataata tttcccatca tagcctgctg
      241 gggaagttaa gtcgtgaaga agtgatggcg gagtacgcgc cagaagcagg cgaagacacc
      301 attctcacat tattaaatga caaccagctg gcccatgttt cgcgtcgtaa agtggagcga
      361 gacctgcaag gtgtggttga agtgctcgat aatcagggtt atgacgtcat tttattaatg
      421 agtacagcaa acattagtag tatgactgcg cgtaatacga tctttcttga gccgtcgcga
      481 atattgcctc cactggtttc ctctattgtt gaagatcatc aggtgggggt tatcgttccg
      541 gttgaggaga tgctgaccgt tcaggcgcaa aaatggcaaa ttttgcagaa atcgccggta
      601 ttttcattgg gtaaccccat tcatgattca gaacaaaaaa tcattgatgc cgggaaagaa
      661 ttactggcaa aaggggctga tgtcatcatg ctggattgtt taggatttca ccaacgtcat
      721 cgcgatttac tgcaaaaaca gctcgatgtt cctgtcttgc tgtctaacgt attgattgca
      781 cggctggctg cggaattact ggtgtaattt tgcgtgacag ccagcgcctc tggcccctat
      841 agtgaagtag atgttcaact accagacagg gccagtttat gcttcaaagt aatgagtact
      901 tttccggcaa agtgaaatca atcggctttt ccagcagcag cactggtcgc gccagcgtgg
      961 gtgttatggt tgaaggcgaa tacaccttca gcaccgctga gccggaagag atgacggtaa
     1021 tcagtggcgc gctgaatgtg ttactgcctg acgcgaccga ctggcaggtg tatgaagccg
     1081 gttcggtgtt taatgttccc ggtcacagtg agtttcatct gcaagttgcc gaacccacct
     1141 cttatctgtg ccgctatctg taattcctcg ccttcccctt gaacgggagg gcatttttct
     1201 gaaatatcct ctctttaacc cataataata tttcctttgc tgcgattttt tcaatt
//
LOCUS       DAPPNJ010000380         1255 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000380 DAPPNJ010000000
VERSION     DAPPNJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.382"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RPM73_004503"
     CDS             complement(<1..902)
                     /locus_tag="RPM73_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX6522749.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPHR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
BASE COUNT          355 a          286 c          300 g          314 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaggtg cctaactgaa tcacagcgag gcggaacgga
       61 cgcgcctctt ttgcccggtg cggtgcaact tctgtaatca gttcacgcag ataactttct
      121 tcgaagcagt gcgcatcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg ttgcaccagc ctgtagcaac gctccgtggt ggttagattt gtggttattg
      241 cggtcaaaca gcaccagatc acccggtgtc agcagggcgt ttaaaaccac tttgttggag
      301 gaggaggtgc cgtttaaaac aaagtacgtt ttatcggcat taaacacttt agccgcatgt
      361 tgctgtgcaa tgcatggcgc accttcatga atcagtagat cgcccattgc tacgtcggca
      421 ttacacaagt cggagcggaa gagcatttcg ccaaaatatt ccacgaactg attgcctgcg
      481 ggatggcgac ggaaaaactc accgccctga tgacccgggc agtcaaaagc gctattgccc
      541 tgattgacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcgt ttcataatgg
      601 ctggcagcgg tttctaactg acgtccgtaa aactctttcc gtgattcgca atgctcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgccgtagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ttgactgata aaataatggc ggcaacgtca atataattac tttcgtcaat attcacgcat
      841 gcacgatgag tggaaaaaca atccgggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcgcgccg gaaaaaagca aaactccaca gctgataaat aaatcagtag tttgcttttt
     1021 gcagatgcaa taacccagac cagcccggta actggcgata ctcatcatac ttcaagttgc
     1081 ctatgctgcg actcgaatta tgtagagttt aaaagggtta gcaatatatg agcaccgagg
     1141 gatacgggta ttaacctgta atgcttaaag tcagggagac ctgtacgctg gggtgccctt
     1201 tagtcagaag actaacgggc attaaagaaa tgaaagtcaa aactattacg atggg
//
LOCUS       DAPPNJ010000381         1235 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000381 DAPPNJ010000000
VERSION     DAPPNJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.383"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>1235
                     /locus_tag="RPM73_004504"
     rRNA            <1..>1235
                     /locus_tag="RPM73_004504"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          313 a          273 c          382 g          267 t
ORIGIN      
        1 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
       61 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
      121 ggaaaatcaa ggctgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
      181 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
      241 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
      301 tggtgccgta acttcgggag aaggcacgct gatatgtagg tgaagtccct cgcggatgga
      361 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
      421 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
      481 ggggtcagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
      541 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
      601 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
      661 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
      721 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
      781 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgttgacccg taatccgggt
      841 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
      901 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
      961 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     1021 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     1081 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     1141 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     1201 tgggtttaga acgtcgtgag acagttcggt cccta
//
LOCUS       DAPPNJ010000382         1208 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000382 DAPPNJ010000000
VERSION     DAPPNJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.384"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(89..>1208)
                     /locus_tag="RPM73_004505"
     CDS             complement(89..>1208)
                     /locus_tag="RPM73_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX6522750.1"
                     /translation="TWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREK
                     AQCRSLAEKIQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTV
                     ERLAAQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHH
                     LHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVI
                     DPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAM
                     RHCYLMCRMTKIFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAK
                     CSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          279 a          356 c          248 g          325 t
ORIGIN      
        1 aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa ataaacaaag
       61 ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa ctcccttgct
      121 ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc ccagtgttat
      181 atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca ataccgacag
      241 tgttattttt aagatccatg attctttctt tagctggctg accttgtcta tcccctgccg
      301 cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatatctta gtcattcgac
      361 acatcaagta acaatgtctc attgcatcgg cggccccatt atgagctccg ccaacaatag
      421 catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca gcaatcaatc
      481 caggtacacc agatacggca taaattagtc cacctgatgt catattctca atcgcatcta
      541 accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata ttccatccac
      601 ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg tagtaccggt
      661 gacggttata gtacagcccc gactccttat catactgctg ccccggcagc cggtacggct
      721 ggtgcaggtg atgcgggttc tcctcattaa gctggttgcc ccattcatca tattccgcgc
      781 tccacgccgt attgccgtct tcactgataa gcgccagcgg caggccccgg tggtcacagt
      841 gataaagatg tatttttcgc tcaggcaaat aaaccggctc tatctgtgcc gccagccgct
      901 ccaccgtcag cccgcactgc gcaagccact ggcggctttc actgctcacg cagtttgccc
      961 ggatttcccc ctccagcctg tccagcagcc ccaccagttc agccgggaac accacaccgt
     1021 gaccgtcctc gctcccttcc tgctgtatct tctccgccag gctgcggcac tgcgctttct
     1081 cccgttcacc gttgtctgtt tcgatgcgga tgagcggcgc gaagctcccc ggctggtata
     1141 ccgtctggat acgcgtggtg tcggtctgta ccgtcgtcag cctgtcgccg tcccagccgt
     1201 accatgtc
//
LOCUS       DAPPNJ010000383         1199 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000383 DAPPNJ010000000
VERSION     DAPPNJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.385"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(84..983)
                     /gene="lysO"
                     /locus_tag="RPM73_004506"
     CDS             complement(84..983)
                     /gene="lysO"
                     /locus_tag="RPM73_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDX6522751.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT          385 a          278 c          301 g          235 t
ORIGIN      
        1 ggataaaagg gatttttcat gcaactttaa gggagattga tttagcgcaa ttttggcggc
       61 agggatctac cgccagagag gtattacgca gagaaaaagg cgatgaggat cggcactaac
      121 aggctaagaa taaaaccgtg aacaattgcc gccgggacca tatccagccc gccagtacgt
      181 tgaagaacgg gcagggtgaa atccattgat gtggcaccgc ataagcccag tgcagtagag
      241 cggctgcggc gaatcagccc agggatcaac ataatagcaa tcagttcacg ggccagatca
      301 ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt cggtcaataa aataccggaa
      361 agagaatacc agccgaaacc ggaggccatt gccagcgcgg tattgatggg gagatcaaga
      421 ataaaggcgt taattaaacc accaattaat gaactgacaa ccaccaccac ggcgacaatc
      481 attccccggc gattaaggac aatctgcttt aaggtcatgc cattattgcg caactgaata
      541 ccaacgagga aaagtagcaa aattaacgtg tattcactgg cttcggtcgc gtgttgtaag
      601 aaagccagtc cactcagacc aatggcaaaa ccaatcacta ctacgccgca cagttttagc
      661 gactccagcg ccatcgcaat acgcgacggg agtttttctt gctgatggtg gttgcgccac
      721 ggcaggcctc gctccagcca catcagggcg gcaatattac acagtaaaat aacggtaata
      781 ctgacggcag aataatgcag aatcgccaac aggttactgg cgaggttatc gagaaacgcc
      841 agactgatac ccataaaaaa gagaataagg taaaccatcc agcttaatag ctgattaata
      901 acttttaacg cagcttgttg gcgaagcgga atgaggtaac ccacaatcag gggaaccaga
      961 atgattaaca gcccagaaaa catgaaaacc cagtccttgc aaagatgaag tcgaaatgcg
     1021 cgatgacaca ctactgaaag cggaaggacg agtaaagttg caattaaaag gaaatgttat
     1081 gcatcagagg gggattttta atcctatctg agtgtttagc attgttgcgc tcctttcatg
     1141 tgcaatgcca tataaagcca ctggcgcagg gagcgcgcag ggggcggcca gtcgccgcc
//
LOCUS       DAPPNJ010000384         1197 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000384 DAPPNJ010000000
VERSION     DAPPNJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.386"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>1197
                     /gene="rhsB"
                     /locus_tag="RPM73_004507"
     CDS             <1..>1197
                     /gene="rhsB"
                     /locus_tag="RPM73_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDX6522752.1"
                     /translation="KTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESR
                     YLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQ
                     PGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGTTE
                     WCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDST
                     AYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKV
                     SAYHTH"
BASE COUNT          313 a          306 c          349 g          229 t
ORIGIN      
        1 agaaaaccga cctcatcccg gaaggggtta tccgcacgga tgatgagcgg actcaccggt
       61 accattacga cagtcagcac cggctggtgc actacacgcg gacacaatat gcagagccgc
      121 tggtcgaaag tcgctatctt tacgacccgc tgggccgcag ggtggcaaaa cgggtatggc
      181 gacgtgaacg ggacctgacg ggctggatgt cgctgtcacg gaaaccgcaa gtgacctggt
      241 acggctggga cggcgaccgg ctgaccacga tacagaacga caggagccgc atccagacga
      301 tttatcagcc ggggagcttc acgccactca tcagggtcga aactgccacc ggtgagctgg
      361 cgaaaacgca gcgccgcagc ctggcggatg cccttcagca gtccggcggc gaagacggtg
      421 gcagtgtggt gttcccgccg gtgctggtgc agatgctcga ccggctggaa agtgaaatcc
      481 tggctgaccg ggtgagtgag gaaagccgcc gctggctggc atcgtgcggc ctgaccgtgg
      541 agcagatgca aaaccagatg gacccggtgt acacgccggc gcgaaaaatc cacctgtacc
      601 actgcgacca tcgcggcctg ccgctggccc ttatcagcaa ggaagggaca acagaatggt
      661 gcgcagaata cgatgaatgg ggcaacctgc tgaatgaaga gaacccgcat cagctgcagc
      721 agcttatccg gctgccgggg cagcagtatg atgaggagtc cggcctgtat tacaaccgcc
      781 accgctatta tgacccgctg caggggcgat atatcactca ggatccgatt ggactgaagg
      841 ggggatggaa cctgtatgga tatcaattga atccgatatc agacatcgac cccctgggtt
      901 tatctatgtg ggaggatgca aaatcggggg catgtactaa tggtctttgc ggcacactat
      961 ccgctatgat aggtccagat aaatttgatt ctatagatag caccgcatat gacgccttaa
     1021 ataaaataaa tagccaatct atttgcgaag ataaagagtt cgctggttta atatgtaagg
     1081 ataatagtgg cagatatttc tcaacagcac ctaaccgagg agaaagaaaa ggatcatatc
     1141 cattcaatag cccttgccct aatggtactg agaaagtatc agcttatcat actcatg
//
LOCUS       DAPPNJ010000385         1194 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000385 DAPPNJ010000000
VERSION     DAPPNJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.387"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(114..297)
                     /locus_tag="RPM73_004508"
                     /pseudo
     CDS             complement(114..297)
                     /locus_tag="RPM73_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          327 a          230 c          254 g          383 t
ORIGIN      
        1 ttccagacag attttatcct gagtgaacag aaagatatta tgataagaat caaacagcaa
       61 tcatatactt gattcatcca ctaaaaatac gtaagtgctc gctatattga taattattta
      121 ttgatgagtg ttacgttcca tggcaggagt tcatcacacg gtagaaagac cattccggcg
      181 gtaagctcag gatatggcgc agatacgctt ctggattgat accgttcagc ctacaggttc
      241 tgattagtcc atgcgcagtg caccgcgctt gccgctgtaa tagctgccga agaacacgga
      301 tatcaaataa atatggatta cttcaatgca caaaaataat gaattttatg tttgtccgca
      361 ttactgtgaa agacagagag attattgagt aatcttattt catagaaaaa tattaatgac
      421 gtcctgtggc gtatgcatga tgcaaggttg aacgcatgta atatgatatt tatcacgtaa
      481 atgcagtgta atcttcttgc attgttttta ggttgctatt ccgtttatct gaggactgct
      541 ggctattgtt gtgcagtttt cctgatacag ctttatgcag gttttccagc attacagtaa
      601 aacccgggaa catcagtgct gctcgtttct ttctcctgta tccactcccg cttatagata
      661 cttccttaaa tattctctcg tctgactaaa gcatatatac ccgttcatgg gatgttggct
      721 gtaatgattt tcctgagcgg attattactg atgaccataa gtatgtctca gcacagtcat
      781 cgtaacgtgc tggttaacct ggccatggta gccaaatcat catcctgtat attctgttga
      841 gggaggcact ggtgattgtt cagaatacgg ctgatgcgaa aaagtgcagg ctgtttcctt
      901 tattggtgtg atgtacagcc tctttacgct ctacttttcc ggggaagaag ccatgctgta
      961 gcactttatt gttacttttc ctggctggac actgtatggt ctggtatccc cccacgtttt
     1021 gaacggaaaa aacagattta aagttgccag tagtctgtaa gtttctgcat cccatttccc
     1081 cacagacagt attctcctgc aatgtgatga atggtttcct ggaacaggaa aggaactaag
     1141 tcagatagtg gagaaagtgg ttaacacgtt gtttttaatt aaaattttgg taat
//
LOCUS       DAPPNJ010000386         1178 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000386 DAPPNJ010000000
VERSION     DAPPNJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.388"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(459..1031)
                     /gene="hdeD"
                     /locus_tag="RPM73_004509"
     CDS             complement(459..1031)
                     /gene="hdeD"
                     /locus_tag="RPM73_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDX6522753.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          402 a          260 c          227 g          289 t
ORIGIN      
        1 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
       61 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
      121 tgcaatgtaa tcaatgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
      181 tagcatcaac gcttacggaa ctctttgttc actttcccac tgcactttta aaacattaaa
      241 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
      301 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
      361 atagtgcgca attgatctac aacgctgcgt agcggagaga gtattaatcg gatcatagtc
      421 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
      481 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
      541 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
      601 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
      661 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
      721 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
      781 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
      841 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
      901 acgaacggga aactgataca cagcaacccg acgataaaca acagcacggc aataaactgg
      961 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     1021 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     1081 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaatta cagtatctga
     1141 tgcatctgta actcattgta ttgaaataaa aatatctg
//
LOCUS       DAPPNJ010000387         1163 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000387 DAPPNJ010000000
VERSION     DAPPNJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.389"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            118..720
                     /locus_tag="RPM73_004510"
     CDS             118..720
                     /locus_tag="RPM73_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDX6522754.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..>1163)
                     /gene="nudI"
                     /locus_tag="RPM73_004511"
     CDS             complement(759..>1163)
                     /gene="nudI"
                     /locus_tag="RPM73_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDX6522755.1"
                     /translation="CPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALR
                     REIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINE
                     EFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          311 a          267 c          248 g          337 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ttcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gca
//
LOCUS       DAPPNJ010000388         1159 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000388 DAPPNJ010000000
VERSION     DAPPNJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.390"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            85..1159
                     /locus_tag="RPM73_004512"
                     /pseudo
     CDS             85..1159
                     /locus_tag="RPM73_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          414 a          163 c          272 g          310 t
ORIGIN      
        1 tcacgatttt catcttggct ttaacgccta caggtgatca aaatttaacc tttggaataa
       61 ctaaaaagat aaaaaaggac ccaggtgagt attcaaagtt tgcttgatta tatttcagtg
      121 atccctgata tacgacaaca aggaaaggtt aaacataaat tatctgctat tttgtttctc
      181 accgtatgtg cagtaattgc aggtgccgat gagtggcagg aaattgaaga ttttggacat
      241 gaaagacttg aatggctaaa gaaatatggt gattttgata atggcattcc ggtcgatgac
      301 accattgcac gcgttgtgag taacattgac agtttggcct ttgaaaagat gtttattgaa
      361 tggatgcagg agtgccatga aatcactgat ggtgaaatta tagcaataga tggaaagacc
      421 ataagaggct cctttgataa gggaaaaaga aaaggagcaa tccatatggt gagtgcattc
      481 tcgaacgaaa atggtgttgt actggggcag gtgaaaacgg aagccaaaag taatgagatt
      541 acagccattc catagttgct taacctactg tatttaaaga aaaatttgat aaccattgat
      601 gctatgggct gtcagaaaga tatcgcttcg aagatcaaag ataaaaaagc agattatctt
      661 ctggcagtaa aaggcaatca ggggaaatta catcatgcat tcgaggaaaa atttcctgta
      721 aatgtgtttt ctaattataa aggcgattcg tttagtacgc aggagataag tcatggaaga
      781 aaagaaacac gtttgcatat tgtcagtaac gtaacgcctg aacttttgtg attttgaatt
      841 cgaatggaag ggattaaaaa agctttgtgt agcattgtca ttcaggcaga agaaagaaga
      901 taaatcagca gaaggtgtaa gcatccgata ttatatttca tcaaaggata tggatgctaa
      961 agaatttgca catgctatca gagcgcactg gctgatcgag cacagtcttc attgggtgtt
     1021 agatgtaaaa atgaatgaag atgccagccg gataagaaga ggaaacgcag cctaaataat
     1081 atctggaata aagaagatgg cactgaattt attaagagat tgcaaagaca ttaagggtga
     1141 agaggaaaag aaagaaggc
//
LOCUS       DAPPNJ010000389         1155 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000389 DAPPNJ010000000
VERSION     DAPPNJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.391"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(606..845)
                     /locus_tag="RPM73_004513"
     CDS             complement(606..845)
                     /locus_tag="RPM73_004513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522756.1"
                     /translation="MEREFPEALERMKERLPLYLIMVLENQYNRLNELDSLIEDIEKQ
                     LTSVAKQNETCKRLLDIPGVGPLIATAAVAITGEA"
BASE COUNT          284 a          298 c          253 g          320 t
ORIGIN      
        1 tggcgggata tggggcttta tatgaatgca tcccgtttac acagcttcca ttgttctggc
       61 attgacggta ggttgcagcc tttatatccg gcattattgc agccataaag ctgcgagccc
      121 ctatgtaatt cgctgactca cacttcattt actgcgcggg attcaagcca aaaaaacatt
      181 caggtttagt gagtcccggt cttacctgtc tttgtcatca ctacggctgc cctcgcaacc
      241 tttcagcatc cccaattttg ttaagagttc attgttgaac ggtatcagcg attaatatgg
      301 tctgatactg acatggttcc cgtcatactt acgaccatgt gcggctattg cccacactgt
      361 tcgtgccagc ttgtttgcca tggcgacgtt gccacgccga ccggacgacg ttttttcagt
      421 tcggttatcc acggcccagg ttctctggtc agtaatgctg ccgcccttgc accatgaata
      481 aacagggttc gcagatattt atcaccacgt ttgcttatcc ccggcaggcg gattttccct
      541 catgagccag tctgtcttgg caccagacca gcataagccg cgaactctcg ccctgattta
      601 aacgcttatg cttcccctgt gatagccacc gctgccgtcg caataagtgg cccaacgccg
      661 ggaatatcca gcaaccgctt acaggtctca ttctgtttcg ccacgctggt aagctgcttt
      721 tcaatatcct ctatcagtga gtccagctca ttcagtcggt tgtattggtt ttccagaacc
      781 ataatgagat acagtggcag tctctctttc atccgctcca gcgcttcagg gaactcccgt
      841 tccatcgctg cccggccttt gtggatggtt ttaccaaact ccagtagcgt cccgtgcagg
      901 gcattaacgg tctaacatcg tggcgttgac accaataaaa ggagaaataa aaatacctac
      961 cataataaat ggcagataaa actgtgataa gggaggaaaa caggtaggtg tatgctctcc
     1021 tgtcggtcat caggtaatct gatacaagaa gatactgttc agttaaaccg cgggagataa
     1081 tctgttatga aatatgtgcg tcacttctca gaaatcgtgt aataacaaac aaaattaatc
     1141 aaaagaaatt catcg
//
LOCUS       DAPPNJ010000390         1134 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000390 DAPPNJ010000000
VERSION     DAPPNJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.392"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            104..1078
                     /gene="cysB"
                     /locus_tag="RPM73_004514"
     CDS             104..1078
                     /gene="cysB"
                     /locus_tag="RPM73_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDX6522757.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          295 a          268 c          270 g          301 t
ORIGIN      
        1 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga
       61 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
      121 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact
      181 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
      241 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
      301 aattcgtatc gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggaga
      361 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta
      421 cgcattacca aacgtcatca aaggctttat tgagcgttat cctcgcgttt ctttgcatat
      481 gcaccagggc tcgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
      541 tatcgccaca gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg
      601 gaatcgggct attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat
      661 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
      721 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgttttca cggcaacgga
      781 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
      841 ggcggtggat ccggtcgccg atcccgacct tgtgcgcgtt gatgctcacg atatcttcag
      901 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga
      961 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt
     1021 gcgctccaat gaagaaattg aggtcatgtt taaagatata aaactgccgg aaaaataatt
     1081 tcttgcgtta ttttcggcac cttttatgta gcgaaggtgc cggaatatat tctc
//
LOCUS       DAPPNJ010000391         1129 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000391 DAPPNJ010000000
VERSION     DAPPNJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.393"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(333..830)
                     /locus_tag="RPM73_004515"
                     /pseudo
     CDS             complement(333..830)
                     /locus_tag="RPM73_004515"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          327 a          230 c          230 g          342 t
ORIGIN      
        1 atttaatatt tattaaatgt atacaatatt aaaataaaaa gtcatgttac attgatcaca
       61 tttttaatag aaacatgatt tattataata cataacatgg atattcatgt aagttatatt
      121 gacatcttat tatctactca gacgtgatga gtatatcatt ggcgttattt ccatagccgg
      181 agatcggtga gttacggcgt ttttcaatat ggttgtcgcc tgatgtaaca aaattacgtt
      241 atgccgcttc tttttctgga ttcaggtaga cttctgatat atagcccagt tacgtagatg
      301 cttgctccaa cgttcaggat gttgctcgcg tgctagctgt tataccactt ttctgactcg
      361 gctgatgtga catattttat cccaccgtgc ttatgttcaa agttgtacca gtttacaaaa
      421 ccgtttaccc atactctgac ttcatcaata gtacaaaaac cgttctcagg ccaccaggga
      481 caatatttga gagtccggaa caaagattct gagtaagggt tatcattgct gactcttggc
      541 cgggaataag aacttagcac accaagcgca tacattttcg ccaacaacgt gcaactgcgc
      601 atgggaccac cgttatcaga atggagtacc agattgttgg atgaacattt ttcattccat
      661 actgcacgct ggagtagttc tgctgcatgt tttccagact cgctggcaaa tacctctgca
      721 gcaataattt tgcgactgta aatatcgacg atcatataga ggtaaaaatg ctggcctctg
      781 acccaggacg gcaaatagct gatgtcccaa ctccagactt gattcggcgc aacggccctg
      841 agtacatcag gccgcttata ctggcattaa cgggatcttt tcgtcaactg gttggctgat
      901 ttcagtacac gataaaagtt tgattcagaa gcaatataaa ttcctctgtc tgccagagtt
      961 ggtacgatca catttggagg taaggaagcg aactctggcg attacaatgt agcatagcgc
     1021 caggttatcg ctcgccacgg gaatatctgc ggcagcgagc cagtaatagg ttaagtgata
     1081 acagatgtct tgaaatatat tgaaaatcaa cctaaatatt tttgactat
//
LOCUS       DAPPNJ010000392         1114 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000392 DAPPNJ010000000
VERSION     DAPPNJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.394"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..443
                     /gene="tnpA"
                     /locus_tag="RPM73_004516"
                     /pseudo
     CDS             1..443
                     /gene="tnpA"
                     /locus_tag="RPM73_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(461..>1114)
                     /locus_tag="RPM73_004517"
     CDS             complement(461..>1114)
                     /locus_tag="RPM73_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDX6522758.1"
                     /translation="FAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATG
                     KPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQ
                     RKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLY
                     FEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          294 a          264 c          247 g          309 t
ORIGIN      
        1 cctgatgcat gttcacctgg tctttgtcac cagataccga cgccagattt tttgacgcga
       61 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
      121 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
      181 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      241 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      301 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      361 ctggtcaggt ggaaaaccgc accttatatc cccgccctga aggatggggt tttacggcac
      421 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
      481 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
      541 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
      601 tattagtcgc tgcatcaatc agctcacctt caaaatagag gcgagtatcc atggtacggt
      661 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
      721 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
      781 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
      841 tatagttcaa aattttatcc agaactttct gccctacctg ggtagaaggt tttggcaccg
      901 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
      961 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     1021 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     1081 ccaacaagcc gcataaagct gcaactttcg caaa
//
LOCUS       DAPPNJ010000393         1103 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000393 DAPPNJ010000000
VERSION     DAPPNJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.395"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            183..>1103
                     /locus_tag="RPM73_004518"
     CDS             183..>1103
                     /locus_tag="RPM73_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HDX6522759.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAY"
BASE COUNT          265 a          274 c          323 g          241 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg
      601 cgctgacaga tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga
      661 gaccgagcat tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc
      721 agataaaaga cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa
      781 gcatcccggg tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg
      841 accgcttcgc ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg
      901 aatcaggtag cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc
      961 gcagggcgtt gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt
     1021 tccgcgaccg tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc
     1081 gcaagctggc acaggtggcg tat
//
LOCUS       DAPPNJ010000394         1093 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000394 DAPPNJ010000000
VERSION     DAPPNJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.396"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            70..1069
                     /locus_tag="RPM73_004519"
                     /pseudo
     CDS             70..1069
                     /locus_tag="RPM73_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          334 a          208 c          288 g          263 t
ORIGIN      
        1 tatcaactgc ttacaaccaa accggcgtgt ggaggtagag gtggatggta tcattagaat
       61 aagtgatgaa taagtagaac tcctcaggaa gaaatcgtca ttattcagca gacaatagag
      121 ccaggagtga ctgtgtccct tatggcgcgt ttgcatggta ttcagcccag ccagcctgtt
      181 gtttaaatga aaaacaatat aaggaaggag gtctccctgc tgttgctgcc ggagaacagg
      241 ttgttcctgc tgccgctata aaataaataa aagagttaca gcgcttgctg ggtaagacaa
      301 ccatggaggt tgatatcatc aaagaagctg tggagtataa ttagtcgtga aaatcgatag
      361 agcatgcgcc ttggtgccca acggataagg aacaactact gtaagctgta ttctgggcgt
      421 gtcgcgtgct caactgacac aacggatgaa aacatctggt aataagccgg ataaacgtcg
      481 tcgcagacgc gatgatgctg cggatgcgga agtgttagca cggatatgcc gacatatggt
      541 tatcgtggtg tctgtgccat tctcagataa cagtcccgta acgaaggact gctgtttgtg
      601 aatgcaaagc gggtttatcg gataatgacc gaaaataacc tgttgttaca tgacgaacct
      661 tcattgccac agcggaaaca tataaaggtc gaatatcggt gaaggaaagc gaccagctct
      721 ggtgttacga tggctttgag ttaggttgcg atgatggcga aaaactgcgt gtcacgttcg
      781 cactgaactg ttgcgatcgt gaagccataa actgggccgc aagtacgtga ggttatgaca
      841 aaaaacagtg caggatgtca tgttaggagc gatagataac ggaatggcag aaaggttttt
      901 gaaaacgatg aaggaagact atatcgtttg catgcacaaa ccaaatgtaa gaccagcttt
      961 gcataacctt gtagtggcga tcgagcatta caatgaaaac aatccgcata gtgcgttagg
     1021 ttatcgctcg ccacggaaat atcgacgtca gcgggtaacg ttaacttaag atacaacaac
     1081 tgtctgaata tag
//
LOCUS       DAPPNJ010000395         1060 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000395 DAPPNJ010000000
VERSION     DAPPNJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.397"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..657
                     /locus_tag="RPM73_004520"
     CDS             <1..657
                     /locus_tag="RPM73_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX6522760.1"
                     /translation="VPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVAL
                     LVRFEKLLESDAPLAEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMF
                     RKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWG
                     RAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL
                     "
     gene            654..>1060
                     /locus_tag="RPM73_004521"
     CDS             654..>1060
                     /locus_tag="RPM73_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX6522761.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMYSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELFGASLSPVQIGLATIGVLWICT"
BASE COUNT          238 a          264 c          295 g          263 t
ORIGIN      
        1 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
       61 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa actgcagcaa
      121 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
      181 gtgctgcctt ccatctacaa acagcatgaa gagcgctaca ccggttatac cctgcgtcag
      241 ttgtgtcagg aaatgcacga tttgtatgcc cgccacaacg tgaaacaact gcaaaaagag
      301 atgttccgta aggaacactt cccacgcgtc agcatgaatc cacaggaagc caactacgcc
      361 tatttacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
      421 gagggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga gatctggggt
      481 cgtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
      541 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtctggtgc
      601 tatgtcatca agccccgtga tgcgcaaagc accctgttaa aaggggaaaa attatgagtc
      661 aggctaaatc gaacaagatg ggcgttgttc agttaaccat actgacgatg gtcaacatga
      721 tgggctctgg catcatcatg ctgccgacta aactggcaga ggtcgggacg atctcgatta
      781 tctcctggct ggttacagct gtaggctcaa tggcgctggc ctgggccttc gcaaaatgcg
      841 gtatgtatag ccgtaaatca ggtggtatgg gcggttatgc cgaatatgcg ttcggtaaat
      901 ccggtaactt tatggcgaac tacacctacg gtgtctcgct gctgattgcc aatgtcgcca
      961 ttgcgatttc cgccgttggt tacggcaccg aactgttcgg cgcgagtttg tcgccagtgc
     1021 agattggtct tgcgaccatc ggggtgctgt ggatttgtac
//
LOCUS       DAPPNJ010000396         1055 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000396 DAPPNJ010000000
VERSION     DAPPNJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.398"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(65..949)
                     /gene="xapR"
                     /locus_tag="RPM73_004522"
     CDS             complement(65..949)
                     /gene="xapR"
                     /locus_tag="RPM73_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDX6522762.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT          285 a          284 c          226 g          260 t
ORIGIN      
        1 tgcgaaattg cctgatgcgc ttcacttagc agacgactat ttccggcaat tcctgtctcc
       61 tcacctactg tgtcaatgca gccaacagct taaccatcgc gggcgtcacc tgctgtgttt
      121 cataaacaat atataaatct gcagggatgc gctgtttgag cggacggaaa atgacacctg
      181 gccagttcat ttgtgcgtag ctgtccgcta tcaatgtgat accaataccc atactgacca
      241 tggcgagtac cgtttgcggt tcattaactt cgcgaataac aaccggtgaa aatcccacct
      301 gctggcaaac tcgctgcaaa aaatcccagt cagtgtacac gggcggcatt gtaacaaaat
      361 actcgtcacg tagcgcttcc agcggaacgg tggaaaatga tgagagatga tgctcttcag
      421 gcatcgccac cagaaacgcc gattcatgca accgtaagct ggtaaaacct gtcggtggtt
      481 ctgtcgccat tcgccagatc ccggcatcga gttcacgtcg ttccagcagg gccatttgca
      541 tcgcgggcat cttttcgcga aaaagaactt caacgttagg attttccctg aggaatcgcc
      601 gcataaccgg gcgcatccgt ccccacatag ccgttcccac tacgccgagt tcaatccgcc
      661 ctgcttctcc ccgacctatt tgttcaaccc gagccaatac attattagca ttcaccagca
      721 atcgacgcga ttcttccatc aagattttgc ccgcgtgtgt cagtacgacg ctgcgcgaat
      781 ggcgaataaa aagctgcgtg ccgagttgat tttccagctc tttaatatga atactgagcg
      841 gaggctgaga catatttaaa cgcgctgctg cgcggccaaa atgcaactct tccgctacgg
      901 caagaaaata acggagcaac ttaagatctg ttctgtatac gcgttccata attaagtgct
      961 ccatataata aatacatcgt attaaattat catggcataa agtatttcac caccagatat
     1021 ccgacataca taaacacaat ggataatata cctgg
//
LOCUS       DAPPNJ010000397         1054 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000397 DAPPNJ010000000
VERSION     DAPPNJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.399"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            61..1005
                     /gene="leuO"
                     /locus_tag="RPM73_004523"
     CDS             61..1005
                     /gene="leuO"
                     /locus_tag="RPM73_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDX6522763.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          285 a          222 c          253 g          294 t
ORIGIN      
        1 ggattatttc tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt
       61 atgccagagg tacaaacaga tcattcagag acggcggagt taagcaagcc acagctacgc
      121 atggtcgatc tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacattacc
      181 cgtgccgctc atgttctggg tatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg
      241 aaggtgatgt ttaatgacga gctttttgtt cgttatggcc gtggtattca accgactgct
      301 cgcgcatttc aactttttgg ctcagttcgt caggcattgc aactagtaca aaatgaattg
      361 cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc
      421 ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat
      481 atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag
      541 gaaacggagt ttgtgattag ttatgaagac ttccatcgtc ctgaatttac cagcgtgcca
      601 ttatttaaag atgaaatggt gctggtagcc agcaaaaatc atccaacaat taaaggcccg
      661 ttactgaaac atgatgttta taacgaacaa catgcggcgg tttcgctcga tcgtttcgcg
      721 tcatttagtc aaccttggta tgacacggta gataagcaag ccagtatcgc gtatcagggc
      781 atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc tattgcgccg
      841 cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc gctgccgtta
      901 aaacaaaaca gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc
      961 catcagtgga tggaagaaca attagtctca atttgcaaac gctaactgaa tgcagaatag
     1021 gtcagacatg aatgtctggt ttattctgca tttt
//
LOCUS       DAPPNJ010000398         1029 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000398 DAPPNJ010000000
VERSION     DAPPNJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.400"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(376..662)
                     /locus_tag="RPM73_004524"
                     /pseudo
     CDS             complement(376..662)
                     /locus_tag="RPM73_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(828..1010)
                     /locus_tag="RPM73_004525"
                     /pseudo
     CDS             complement(828..1010)
                     /locus_tag="RPM73_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          275 a          258 c          163 g          333 t
ORIGIN      
        1 tatatccaaa tattaatatc aaaacatcaa ctttttccat ataccttgct gtgaagatga
       61 tgggcataca tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca
      121 ttattcctcc tacaatcaat gtgcaattac atttaaacac acctcaattt ggccggacat
      181 ataaatatct aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaagg
      241 ttcatcgtaa aacattcccc atacttatca gtccgttccg cgcaaggtag ctcattgcct
      301 tatctggcaa cctgtaatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat
      361 agttaatcca ttaatctagt tgccggatgt tggtggattt tcgcgtttta gttgttcata
      421 aaagtgcaca gctttaacca gttcttctga tgtaaccggg actggtgagg cagtgaataa
      481 ggcctgaatt tgatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca
      541 gtcaccagac cactacttcc cctgaaagtc cgtaacgctt ttttcacgta gcgatatcgc
      601 catgccactg gttttgcttg ccccgccgtt tcatgccctt cctgataatt aatctcgctc
      661 attcatcgcc ccactcatca caatatgctt cgaccggagt ttttcccgct tcatagtcat
      721 cacgctagga ttcgacatca gcagcacttc caccgcgtaa ttcaccatag tccattaata
      781 gttcatcccg ctcttcaaaa ctgatgttat atttagctga acaaaaatca gccactttgt
      841 tcttcctcat cgtcttttat ttcgtggtat gagtaattgc agtagttaaa gaaaatttct
      901 tatgctccgt catgaatttc atcaggtgtt gcgtcatcgt ccacttcgaa tacatcctca
      961 aaatctccac cagctatttc cgtttcaata ataattttga actttcgcat ttcactaccg
     1021 ccctttcgg
//
LOCUS       DAPPNJ010000399         1017 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000399 DAPPNJ010000000
VERSION     DAPPNJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.401"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..434
                     /locus_tag="RPM73_004526"
                     /pseudo
     CDS             1..434
                     /locus_tag="RPM73_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            448..688
                     /locus_tag="RPM73_004527"
                     /pseudo
     CDS             448..688
                     /locus_tag="RPM73_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            708..995
                     /gene="sctI"
                     /locus_tag="RPM73_004528"
     CDS             708..995
                     /gene="sctI"
                     /locus_tag="RPM73_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDX6522764.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKEGLTPEKLLVLQDHVLNYNVEVSLVEH"
BASE COUNT          365 a          160 c          204 g          288 t
ORIGIN      
        1 aacttcaact attagatgcc agtaatctaa gattgacagt gagtaagcaa cggaacgcta
       61 tcacacagca actaatcgat aatcttatta aaggattact gcaaactatg ccatatgcca
      121 gcaatataag tattgcggta ttagatgata atgtattgga aagtcaggct attgaaacgt
      181 tgtcagcgat aggtctttct tatgaaaaat ataaaacagc taacaatgtg tatttcaata
      241 tcattggtac gttaagtgac agtgaattaa ataaaattaa taactatgtt gacgaatatt
      301 ataaacaata gggtaaacaa tatgtaagat ttaatgtgag tttgaaaaat caggacacaa
      361 ataatagttc atttagctac ggagataacc gattcgagaa gtctcaaggt agcaactgga
      421 catttcagga ataattgagg aggacttatg gcagattgga atggttatat tatggatatc
      481 agcaatcaat ttgatcaggg cgttgatgat ctgaaccagc aagttgaaaa agcgttggag
      541 gatttagcca accaatccct ccgacacgaa atttcttgcc gaatatcaaa gtgcattagc
      601 tgagtataca ttatatcgaa atgcgcaatc taacgttgta aaagcgtata aagatcttga
      661 ttcagcaatc atacaaaact tccgctaaac tggataagga ttttattatg tcagtaagca
      721 atatgccacc tattgataga gctgagcaat caactgctca tgaaatacag caagcgaaag
      781 tgattgattt aaatgaccgg gttctcaatc ttgacaaccc ggatgataaa atgatctccg
      841 cttttgccaa ctatgctgta caaactgaaa attggcagca aaatgcgtta caagcattaa
      901 ggtctgataa agaggggtta actcctgaaa aattattagt cttgcaggat catgtgctta
      961 attataatgt cgaggtttca ttggtggaac attagcaaga aagattgtcg ctgcagt
//
LOCUS       DAPPNJ010000400         1011 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000400 DAPPNJ010000000
VERSION     DAPPNJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.402"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            44..274
                     /locus_tag="RPM73_004529"
     CDS             44..274
                     /locus_tag="RPM73_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522765.1"
                     /translation="MKLFIFSLFVIVTSMVSGIAIAELSYFILLIIKYLAYGEVDFRW
                     SEVLRGLRMGCVGGGILGFGIVLFRFLGIKGF"
     gene            742..984
                     /locus_tag="RPM73_004530"
     CDS             742..984
                     /locus_tag="RPM73_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HDX6522766.1"
                     /translation="MGAYLGSKVKGEDPTVPMVGNAIGTVLGNKAGEKFTKEMLSKGY
                     SPVTSDVIGTVSGGTIGTAIDFGIEESQKKGDSNEK"
BASE COUNT          288 a          159 c          268 g          296 t
ORIGIN      
        1 tacaaaaagt cattaatgat aaattgaaga aaggagagcg tgaatgaaac tatttatttt
       61 cagtctcttt gtcatagtta cctccatggt ttcaggaatt gcaattgctg agttgtctta
      121 tttcatattg cttatcatta aatacttagc gtatggggaa gttgatttta gatggtccga
      181 agttttaaga ggtctaagga tggggtgtgt gggaggtgga attctaggtt ttggtatagt
      241 cttgtttcgc tttcttggca taaaaggatt ttaagtgaag ataaggtacg atttgttgtt
      301 gagaataatt caatgtctgg cctggaaggg tttgggaccg gcttccggag ttatgttcag
      361 gcccagggga cgctggtaaa taataccaat ctcactgatg aaactggcaa agtgctgaat
      421 ccggcaacgc cagaggaaat taagtatgct tctgataaac ttgcaacagg taatcttccg
      481 gaaggacagg atcctgtcag aggtttactg attgcatggg gagcaggtgc ttctgttctt
      541 ggtggcgaac ttatagtccc agccgcgggt accgttgctg ttattggggg tagcctttta
      601 ggaggtgtaa cagatggcat taagcagtat ttaaccctta agccgggtga gaaatatagt
      661 gctacggata ctcttattgc cgcaggtgaa ggtgggctta cacagggtaa aagtgtcatt
      721 ttcagcacat ttattaacac aatgggagct tacttaggaa gtaaagttaa aggcgaagac
      781 cctacagtgc caatggtggg gaatgcaata ggtactgttc tgggtaacaa agccggagag
      841 aaattcacca aagaaatgct atcgaaggga tactcgccag taacttctga tgttattgga
      901 actgtctctg gggggactat aggtacagct atcgacttcg gcattgaaga atctcagaag
      961 aaaggagata gtaatgaaaa ataaatctcc tgacttatgg ctgttattct a
//
LOCUS       DAPPNJ010000401         1005 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000401 DAPPNJ010000000
VERSION     DAPPNJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.403"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            173..454
                     /locus_tag="RPM73_004531"
     CDS             173..454
                     /locus_tag="RPM73_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522767.1"
                     /translation="MKPVMPELITVEELAFALKKTVKSIRSDATRNPKCLPPRCRLPG
                     NKRLLWRREDVKEWIDNSVESELENRDLMQRAEMVQKKRGRPPKIKPKE"
     gene            738..>1005
                     /locus_tag="RPM73_004532"
     CDS             738..>1005
                     /locus_tag="RPM73_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HDX6522768.1"
                     /translation="MTVSQTRKNSTPIKVYCLPDEKAQIQENAEASGLSVASFIRKVA
                     MGYQVESIVDIDQVVELSRVNADLGRLGGLLKLWLANDPRTVDFS"
BASE COUNT          312 a          185 c          221 g          287 t
ORIGIN      
        1 tagagctggt ccagtggttt tttatttcct ctagctgttg aggtgttgac agaaatattt
       61 caggtgataa tttttgggta ttctcaattc ttaacgttgg tttgcaaaat ttagcggcta
      121 aaattatagc ggctaaaatc ctgaaaatat tttctgagag ataggttctg ctatgaaacc
      181 agttatgcct gaacttataa ccgtcgaaga gttagctttt gctttgaaga aaacagtgaa
      241 aagtatccgt agtgatgcaa ctcggaaccc taaatgcctc cctcccagat gccgtcttcc
      301 tggcaataag cgacttttgt ggcgtcgaga agatgttaaa gagtggatag ataattctgt
      361 tgaatcagag ctggaaaacc gcgatttaat gcagcgtgct gaaatggttc aaaagaaacg
      421 aggtaggcca ccgaagataa aaccgaaaga ataattatct taaattttgg tatttaaatc
      481 agtttccttt cagaaaacac atatattaca cacttaatat atgaataaac ctgcagaaat
      541 acatcaaaaa aacacactgc ttacaccgaa atacacaatg ggtacacaga tatacaacgg
      601 caacagtggg gcaggacatg taaagtaggc ccaccgtaaa cggcgcttct actttacctt
      661 cccttattcg cagctgggct gctcaatgga gtcctgctct gcgaggctga tcggctaccg
      721 cctgtatgga gaatgatatg accgtttctc agactagaaa aaatagtaca ccaattaaag
      781 tttattgttt gccagatgaa aaagcgcaaa tccaggaaaa tgcagaagca tctggcttaa
      841 gtgtagcgag tttcattcgt aaagtagcaa tgggttatca ggttgagtct attgtagata
      901 ttgatcaggt tgtagagctt agccgagtaa atgctgatct tggtcgactt ggtgggttac
      961 ttaaactgtg gttagctaac gatccacgca cagttgattt ttcac
//
LOCUS       DAPPNJ010000402          989 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000402 DAPPNJ010000000
VERSION     DAPPNJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.404"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
BASE COUNT          268 a          189 c          214 g          318 t
ORIGIN      
        1 tcattaaagt tcttctcgaa agctataaaa aactggatac ccagtcaaag aaggagattt
       61 atttgacttt ttccaagtcg acagatagag gcctttatcg atatataaaa agaatagctt
      121 catcagtact cgataactta atggttaaaa tataaatgcg tgcgcgcgag atacaatttc
      181 tcgcgcattg tgtaaccggc gacaaaaccc tgttatcatt atagcgaccc gtttagatgg
      241 gccatttgtg ccccaacagc ttctgggtat agatttcctc ggccctgtat agatactttg
      301 ctaaagagta ttttaatggt attttgctta cgtaccattc ttctaacgaa tgtctgcctt
      361 atcctgtgag ctgccttcgc tcagcattgg cctgtttctc gttgatttat atactgcgac
      421 actaacatag gcggatatgt ccgctcctcg ctcataacag acattcacta cagttatggc
      481 agaaaggtat gcatgctggg tggggaaagt atgaaggaaa agaaaactgc tgcgctgttt
      541 gtcgtcacgt ttatcttcat tggctatgca agtcataata caaggtggga caaaactgag
      601 acacataagg cctcacaatg gcttgcaagg ctttacatgt tttgatgtgg tgggacgtgt
      661 gagcgcagtg ttgatggggt aatgctttga attagaagcg gattcttata attcgtaatg
      721 cgaaggtcgt aggttcgact cctattatcg gcaccatttt ttctaataaa atcatctggt
      781 tactgttgcg ttgttttgct tttcttaaca caaatgtgca tttttgcgcg ttgtgagtgt
      841 ggcaaaattg tggcaggttt ctttttggaa tgagaagata cgctacacat ctcaatccat
      901 ctcaacatac cttgaaccag ctcgtagatg ctgtaagatc tgtgaagagt ttttctactt
      961 tacctaagtt cataatatcg gattagttt
//
LOCUS       DAPPNJ010000403          986 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000403 DAPPNJ010000000
VERSION     DAPPNJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.405"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     repeat_region   209..847
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=209..237
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          257 a          244 c          250 g          235 t
ORIGIN      
        1 gtggcattaa tttttcgctg gagtgaaaat tattgcggta aagtttggta gattttagtt
       61 tgtatagagt tattttaaat atttaccttt ttaatcaatg gattaagtgc tctttaacat
      121 aatggatgtg ttgtttgtgt gatactataa agttggtaga ttgtgactgg cttaaaaaat
      181 cattaattaa taataggtta tgtttacaga gttccccgcg ccagcgggga taaaccgctg
      241 acgcaacgat tgaccgtgcc gccgggttgg agttccccgc gccagcgggg ataaaccgcc
      301 gccgttatat ggagcgattc cggtttggta gagttccccg cgccagcggg gataaaccgt
      361 attcccccag tcacccccgc caccaaaatc agagttcccc gcgccagcgg ggataaaccg
      421 atttttcaga ctcatcaaaa taatccttta atgagttccc cgcgccagcg gggataaacc
      481 gaacgtgaga tacgcctccc agcggcgctc ggtgagttcc ccgcgccagc ggggataaac
      541 cgccacacac gtcgagctgg tgggggttaa tgctgagttc cccgcgccag cggggataaa
      601 ccgacacctc tactgacgtt cctgaacaag aagatgagtt ccccgcgcca gcggggataa
      661 accgctgggc gagggagaaa cgccagcgga gcagtagagt tccccgcgcc agcggggata
      721 aaccgggatc acaagggcgg ctatcagggt ggacggagag ttccccgcgc cagcggggat
      781 aaaccgcctc tcaatttgta acgtctgcaa tgtcaggcga gttccccgcg ccagcgggga
      841 taaaccacca tataacccgt tatctctttc tcaagttttt atattagcag tacttgtaat
      901 aagcaacata tccacgtaac acctcatgtt caaaatagtt ctccatgcca gagaggttca
      961 caattatcga tacaaaaaat taaatt
//
LOCUS       DAPPNJ010000404          965 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000404 DAPPNJ010000000
VERSION     DAPPNJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.406"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..628
                     /locus_tag="RPM73_004533"
     CDS             <1..628
                     /locus_tag="RPM73_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HDX6522769.1"
                     /translation="LASIILLVLTNKLPLLLSGVVGGASLQGIGGFGAGMITGAATTA
                     ISGAGAMAMSATAQVSGGASALKAAFESAQAAMAEESGSGDKGGLGGGFGGGEDTGSV
                     DTSGGQPSGGSGGSSAGSNTGSVSGGSQGFASSFSRAGRMASHMASGLSNGASEYQMM
                     KGSANSIRTQQTVGGEIANQIRKHTADRQSNHEQDEFEGDSFTGSKKS"
     gene            698..910
                     /locus_tag="RPM73_004534"
     CDS             698..910
                     /locus_tag="RPM73_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522770.1"
                     /translation="MADYNSFVLEGIKIIFADNNKEFIKEICEPLINILCIEEYYSAT
                     GIYNYDDAYEQTELLKNALRDYKMAH"
BASE COUNT          272 a          169 c          258 g          266 t
ORIGIN      
        1 tctggcgtca attattcttt tagtattgac gaataaatta ccgttgttgt tatcaggcgt
       61 tgttggagga gcgtccttac aagggatcgg tggttttgga gcaggtatga ttactggcgc
      121 tgctactact gcaatcagtg gcgctggggc aatggcaatg agcgctacag ctcaagtcag
      181 tggtggtgct tccgctctaa aagcagcatt tgaatctgca caagcggcga tggccgaaga
      241 atctggttct ggtgataaag gtggtttagg tggaggattt ggcggcgggg aagataccgg
      301 ttccgtagat acttctggag gacaaccatc aggcggttcc ggtggttctt ctgcagggag
      361 caatacaggt agcgtcagcg gaggaagcca ggggtttgct tcatcattct cacgagctgg
      421 ccgcatggct tctcacatgg cgagcgggct gtctaatgga gcatcggagt atcagatgat
      481 gaagggaagt gctaattcta tccgaacaca acagactgtt ggtggggaaa tagcgaatca
      541 aatacgtaaa cacacagccg atcgtcagag taaccacgaa caggatgagt ttgagggtga
      601 tagttttact ggcagtaaaa aatcttgatt tagacgcgtc agttagcgcg gtaacgttaa
      661 ctgacgcttc cactaaacac ctgtaaggag gtggataatg gctgattata atagctttgt
      721 tttggaagga ataaaaatta tttttgccga taacaacaag gagtttatta aagagatttg
      781 tgaaccattg ataaatattc tatgcatcga agagtattat tctgctaccg gaatttataa
      841 ttatgatgat gcttatgagc aaactgagtt acttaaaaat gcattaaggg actataaaat
      901 ggctcactag tgaaaagagg ctaacttgta ttagctcaaa cttgatctta cacttttact
      961 gcata
//
LOCUS       DAPPNJ010000405          951 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000405 DAPPNJ010000000
VERSION     DAPPNJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.407"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(244..876)
                     /locus_tag="RPM73_004535"
     CDS             complement(244..876)
                     /locus_tag="RPM73_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDX6522771.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          240 a          212 c          201 g          298 t
ORIGIN      
        1 gcgccggtga tttacaagta agggtatgat tacgcttttt aaagcgtacc ataaaacata
       61 acataataaa tgatttgtga tgacacggga ttattgggat ttactttgag gaagataaaa
      121 tgcgtgttta tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg
      181 gtcataaaaa attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca
      241 gcattagttc acaaacagtg cttcgctgtc gacgatgatt ccatcttccg cacctttaag
      301 cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat cagcaagtgc
      361 attacgagtc tgtttaagcg gcatcagata ttggttttcc attacgccaa caccggccag
      421 gcgaagttct tccattgcgc cctttttatc ccctttcgcc attttttcat tggcaatttt
      481 tatcgcggct tctttttcgg gagttgctac ataactctca gaaattccga cagatgcatt
      541 aatgacaata tattggtcat cattgagatt ggtcttttta cctggcttag cgaactttgc
      601 ccattcggta ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt cttttgcctt
      661 ttctggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa
      721 accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt
      781 ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat
      841 tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt
      901 tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga g
//
LOCUS       DAPPNJ010000406          921 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000406 DAPPNJ010000000
VERSION     DAPPNJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.408"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(13..903)
                     /gene="alsR"
                     /locus_tag="RPM73_004536"
                     /gene_synonym="rpiR"
     CDS             complement(13..903)
                     /gene="alsR"
                     /locus_tag="RPM73_004536"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDX6522772.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT          236 a          234 c          200 g          251 t
ORIGIN      
        1 attacctttt tcttatttca gcgcgcctgg tgagaagaaa tcaacaattg cgccggtttt
       61 ttgcatatta atattagctt gttcaatgtt gagctgggcg acagagacaa aaaaagcgtc
      121 cagcaaagtt agttgtaata ttcttgccga ggcattacga cctaataacg gcgtttccgg
      181 ggctggtgag caaataatat aatcggccag tttcgctatc ggtgaatggt agctatgggt
      241 tatacaaata atctttgccc cgttcttttt tgccagttct acggccgctt ttacatcact
      301 ggttcgcccg gaatgggtca ctaccagcac aacatctcct tcctgtaaca acgaagcgga
      361 catcatcatg atgtgagcat caggataggc ctgacagcgt acgccaatgc gcaagaactt
      421 gtgctgtaca tcagcacaga tagcatttga tcctccggca ccgtacaaat cccgctgtct
      481 ggcctgatag aaaaagcggg cggcacggtg gatctcatca acgttgacga tcgactgacc
      541 ttccataatg gtgcgtaaag tgatgttaaa taccttattc accacatcct gcggcgcttc
      601 atcaaaagcc aactcggaag gcaatacctg ttctgactga gaaaaataat cttccagcgc
      661 actgcgtaag ttacgaaagc cgctaaaccc cagcagcttt gataccttaa ctatcatcgc
      721 ttcagatacc gccagagctt ctgcgacatc tttaattgcg ggtgcacaac tcaggttacc
      781 gggtttgagt aaccactcta cgatgcggct ttcattttct gtcattcctt cctgcttcat
      841 tcgcaggtaa ggcgctaacc ctataccgtt cggaagcgct gaatcaaact ctgactggct
      901 catcacgttc tcttgtcctt g
//
LOCUS       DAPPNJ010000407          916 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000407 DAPPNJ010000000
VERSION     DAPPNJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.409"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..787
                     /gene="gadX"
                     /locus_tag="RPM73_004537"
     CDS             <1..787
                     /gene="gadX"
                     /locus_tag="RPM73_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDX6522773.1"
                     /translation="RHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRD
                     TLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKR
                     ALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPS
                     LLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNY
                     YGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          266 a          174 c          207 g          269 t
ORIGIN      
        1 aagacataaa tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga
       61 cctggttttt gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt
      121 agtatcaagg gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct
      181 tcatgcacat aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt
      241 ttttcaggaa gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg
      301 taaaagagca ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc
      361 gctgcttttg aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa
      421 taatatcgcc catgagtgga cactagctcg aatcgccagc gagctgttga tgagtccaag
      481 cctgttaaag aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg
      541 tagaatgcaa cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc
      601 agtgtcctgt ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg
      661 gatgacgccc acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc
      721 ggcaagtatt gttgcgcaag gaaattttta cggcactgac cgttctgcgg aaggaataag
      781 attatagaat tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag
      841 tctgccgtct ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt
      901 gctctcagta agttaa
//
LOCUS       DAPPNJ010000408          912 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000408 DAPPNJ010000000
VERSION     DAPPNJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.410"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..692
                     /locus_tag="RPM73_004538"
     CDS             <1..692
                     /locus_tag="RPM73_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522774.1"
                     /translation="FEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVK
                     EHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRI
                     TIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSG
                     PAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGA
                     NFVIQFNGVEF"
     gene            694..>912
                     /locus_tag="RPM73_004539"
     CDS             694..>912
                     /locus_tag="RPM73_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522775.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVD"
BASE COUNT          297 a          150 c          176 g          289 t
ORIGIN      
        1 attttgaaga aaaagctaaa agtttaaacg cgcttgcaca gttaggtatt agtgttgaga
       61 taatatctca tgaacttgaa gaaatggatt ctatggtaac cagaggacta aactctcttc
      121 ctacttctgt aaaagaacac cctggttttt cattggcgtt aaatgctcac agatcgctta
      181 ctcaacaaat acgtttctta tcacctttga aaatatcagg ttatcaatcc aggcagagaa
      241 taactggaaa aaatatcatg gattatgtcc tgaagttctt tggggagcgt ttcgaacggc
      301 aacgaataac tattgaattt agtgaagagt ttaagcaaat cgcaataaca gatataccat
      361 caaggatcta tcctgttttt actaatatta tcaacaatgc aatgtattgg gtcagtctgt
      421 caaataatag gctcataaag attggttttg tgaattcttt ggttatcata gcaaattctg
      481 gtccggcaat tgataccgat gatatcccgc gactatttga actattttat agcaaaagag
      541 caaatggaca tggggtaggt ctgtatctat gtcgagaaaa ccttgctgtt gcacatcata
      601 aaatatggta ttcagaacct gatgaaggcg ataactattt aataaaagat ggcgctaatt
      661 ttgtgatcca gttcaatgga gtggagttct aatatgacag tggcaaatta taattctctt
      721 gtccagaaaa ctttctgcga aaatgcaatt cgttccgttg tcatgattga tgacgatttt
      781 ctgacgtatt ctgaatcaat cagggcgttg aataacgaag ttgatttaga ctacaacaaa
      841 attgactcat ctaaacgagc cgctactctt gagagctttt ttcaatctaa aaatatgatt
      901 tgtgatgttg ac
//
LOCUS       DAPPNJ010000409          908 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000409 DAPPNJ010000000
VERSION     DAPPNJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.411"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..815
                     /gene="yeeO"
                     /locus_tag="RPM73_004540"
     CDS             <1..815
                     /gene="yeeO"
                     /locus_tag="RPM73_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDX6522776.1"
                     /translation="AGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSII
                     WEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGS
                     ASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVK
                     HVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIM
                     LGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          184 a          179 c          263 g          282 t
ORIGIN      
        1 gggctgggtt gggtttaacc atttctcgtt atattggcgc agttgcaatt ttgtgggtgc
       61 tggcgattgg ttttaatcct gcgctaagga tttcgttaaa gagctatttt aaaccgctga
      121 attttagcat tatctgggaa gtcatgggga ttggtattcc cgcgagtgtc gaatcagtgt
      181 tatttaccag tggtcggtta ttaacccaaa tgttcgttgc cgggatgggg accagtgtta
      241 ttgccggaaa ttttatcgcg ttttcaattg cggctcttat caacttaccc ggaagtgcgc
      301 tcggctctgc ttctacgatc attacaggcc gaaggttggg ggtagggcag atagcgcaag
      361 cagagattca gttgcggcat gtgttctggc tgtccactct tggattaacg gccatcgcct
      421 ggctaacggc tccctttgcc ggggttatgg catcgtttta cacccaggat ccacaggtta
      481 aacatgtcgt tgtgattctg atttggctaa atgctttatt tatgcctatt tggtccgcct
      541 catgggtgct acccgctgga tttaaaggtg ctcgtgatgc ccgttacgcc atgtgggttt
      601 cgatgttgag catgtggggt tgtcgggttg tcgtcggtta tgtgctggga atcatgcttg
      661 gctggggtgt ggttggtgtc tggatgggaa tgtttgccga ctgggctgtg cgggccgtgc
      721 tgttttactg gcgaatggtt actggacgtt ggctatggaa ataccctcga cccgagccgc
      781 aaaagtgtga aaaaaagcca gttgtgtcgg aataaacgac aaaatgcaga ttatttcagc
      841 aaacgatttc aaatttaaaa aacaggcttt gacattgtgg gtgggcatcg ctaatattcg
      901 cctcgttc
//
LOCUS       DAPPNJ010000410          901 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000410 DAPPNJ010000000
VERSION     DAPPNJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.412"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            112..636
                     /gene="aroL"
                     /locus_tag="RPM73_004541"
     CDS             112..636
                     /gene="aroL"
                     /locus_tag="RPM73_004541"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDX6522777.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            686..877
                     /gene="yaiA"
                     /locus_tag="RPM73_004542"
     CDS             686..877
                     /gene="yaiA"
                     /locus_tag="RPM73_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDX6522778.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          251 a          211 c          235 g          204 t
ORIGIN      
        1 tttaagtgga attttttctt tacaatcgaa attgtactag tttgatggta tgatcgctat
       61 tctcatgaca ccggctttcg ccgcattgcg acctattggg gaaaacccac gatgacacaa
      121 cctctttttc tgatcgggcc tcggggctgt ggtaaaacaa cggtcggaat ggcccttgcc
      181 gattcgctta accgtcggtt tgtcgatacc gatcagtggt tgcaatcaca gctcaatatg
      241 acggtcgcgg agatcgtcga aagggaagag tgggcgggat ttcgcgccag agaaacggcg
      301 gcgctggaag cggtaactgc gccatccacc gttatcgcta caggcggcgg cattattctg
      361 acggaattta atcgtcactt tatgcaaaat aacgggatcg tggtttattt gtgtgcgcca
      421 gtatcagtcc tggttaaccg actgcaagct gcaccggaag aagatttacg gccaacctta
      481 acgggaaaac cgctgagcga agaagttcag gaagtgctgg aagaacgcga tgcgctatat
      541 cgcgaagttg cgcatattat catcgacgca acaaacgaac ccagccaggt gatttctgaa
      601 attcgtagcg ccctggcaca gacgatcaat tgttgatttt agagcgccta tacttaacgt
      661 tcatcccgtg aaataaggaa gaacgatgcc aacgaaaccg ccttatcctc gtgaagcata
      721 tatagtgacg attgaaaaag gaaagccagg acagacggta acctggtacc aactcagagc
      781 cgatcatcct aaaccagact cgttgatcag tgaacatccg acggctcagg aagcgatgga
      841 tgcgaaaaaa cgctatgagg accctgacaa agagtgaccg catcagactg ctcggaaggg
      901 a
//
LOCUS       DAPPNJ010000411          870 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000411 DAPPNJ010000000
VERSION     DAPPNJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.413"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            24..638
                     /gene="evgA"
                     /locus_tag="RPM73_004543"
     CDS             24..638
                     /gene="evgA"
                     /locus_tag="RPM73_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDX6522779.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            643..>870
                     /locus_tag="RPM73_004544"
     CDS             643..>870
                     /locus_tag="RPM73_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDX6522780.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQ"
BASE COUNT          272 a          173 c          181 g          244 t
ORIGIN      
        1 cttcattgca aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta
       61 tcgcagcaat tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttgactg
      121 aagggggaag cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc atcattgatg
      181 tcgatatccc cggagttaac ggtatccagg tgttagaaac gctgaggaag cgccaatata
      241 gcggaattat tattattgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg
      301 atgctggcgc taatggattc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta
      361 ttgaagctgc aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa
      421 gtttaacgtc cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc
      481 ggtatattct tgatggcaag gataataatg acattgctga aaaaatgttc atcagcaaca
      541 aaactgtcag cacttataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg
      601 atctttacac attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat
      661 ttttttcttc tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc
      721 gaatatcgtg gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac
      781 aaggaattac gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa
      841 acggctacct tgttgcatac cgattcgcag
//
LOCUS       DAPPNJ010000412          866 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000412 DAPPNJ010000000
VERSION     DAPPNJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.414"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..843
                     /locus_tag="RPM73_004545"
     CDS             <1..843
                     /locus_tag="RPM73_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDX6522781.1"
                     /translation="KAGNGRRPYPLETMLHIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINI
                     EYMKASIRARVEHPFRIIKRQFGFVKARYKRLLKNDNQLAMLFTLANLFRVDQMIRQW
                     ERSH"
BASE COUNT          229 a          248 c          230 g          159 t
ORIGIN      
        1 aaggctggta atggccggcg accttatccg ctggaaacca tgctacacat tcactgcatg
       61 cagcattggt acaacctgag cgacggtgcc atggaagatg ccctgtacga aatcgcctcc
      121 atgcgcctgt ttgcccgatt atccctggat agcgccctgc cggatcgcac caccatcatg
      181 aatttccgcc acctgctcga gcagcatcaa ctggcccgtc aattgttcaa gaccatcaat
      241 cgctggctgg ccgaagcagg cgtcatgatg acccaaggca ctttggtgga tgccaccatc
      301 attgaggcac ccagctctac caagaacaaa gagcagcaac gcgatccgga gatgcatcag
      361 accaagaaag gcaatcagtg gcactttggc atgaaggccc acattggtgt cgatgccaag
      421 agtggcctga cccacagcct ggtcaccacc gcggccaacg agcatgacct caatcagctg
      481 ggtaatctgc ttcatggaga ggagcaattt gtctcagccg atgccggcta ccaaggagcg
      541 ccacagcgcg aggagctggc cgaggtggat gtggactggc tgatcgccga gcgtcccggc
      601 aaggtaaaaa ccttgaagca gcatccgcgc aagaacaaaa cggccatcaa catcgaatac
      661 atgaaagcca gcatccgtgc cagggtggag cacccgtttc gcatcatcaa gcggcagttc
      721 ggcttcgtga aagccagata caagcggctg ctgaaaaacg ataaccaact ggcgatgtta
      781 ttcaccctgg ccaacctgtt tcgggtggac caaatgatac gtcagtggga gagatctcac
      841 taaaaactgg ggataacgcc ttaaat
//
LOCUS       DAPPNJ010000413          823 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000413 DAPPNJ010000000
VERSION     DAPPNJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.416"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            1..823
                     /locus_tag="RPM73_004546"
                     /pseudo
     CDS             1..823
                     /locus_tag="RPM73_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
BASE COUNT          269 a          127 c          168 g          259 t
ORIGIN      
        1 tgcgtcatcg ggatataatt attgcaaaaa tagatttgtt tattcaacga atcacgcata
       61 atttatggca tgcacgtaaa aaaatgtgtt tttaattgag caaataaatg atctcaaaat
      121 gtgggtcaat aagtatattg acgattgcac tgatgaggat ttaaacgatc gtgactttat
      181 tgcatcagtt gttgaccggg ctatttttca tttcgcgatt aatagtatat gtaatcctgg
      241 ggataataaa gatgcgactc ccattgaaca atgtactttt gatgtagaaa ctaagaatgg
      301 ccttccctcc acggttcagc tattttatga ggaatctaag gataatgaac ctttagcgaa
      361 tatacatttt caagcaatag gttctggttt tttaacgttt gttaatgcct gccaggaaca
      421 tgatgacaac agcttaaaat tatttgcttc gctgttaatt tcactttcat attctagtgc
      481 ctacgcagat ttatcagaaa cagtgtatat taatgaaaat aatgagagct acctgaaagc
      541 tcagtttgaa aaattatctc aacgtgatat gaagaagtac ctgggagaga tgaagcgtct
      601 ggctgatggg ggagaaatga attttgatgg ctatctggat aagatgtcac atctggtgaa
      661 tgaaggaacg ctcgatcctg atattttaag caaaatgcga gatgctgcac cacaattaat
      721 tagcttcgcg aagtcgtttg acccaacctc aaaggaagag attaaaatac ttacagacac
      781 ttctaaatta atttatgatt tgttcggggt taaatcggag aaa
//
LOCUS       DAPPNJ010000414          817 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000414 DAPPNJ010000000
VERSION     DAPPNJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.417"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>817
                     /locus_tag="RPM73_004547"
     CDS             <1..>817
                     /locus_tag="RPM73_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDX6522782.1"
                     /translation="GSQNATISIGNSNYKRQIKNVENGIDDYDAVNVSQLKAVSNSAS
                     EAISIVNNANTSINQAVNIANSLNDTVSKGINIGADNGPVVKRELGDTVTVTGDSNIR
                     TKTTSTGVKVALNQNINLGENGSVNTGNTTVNSHGVIINEGPSMTRSGFNAANNRITN
                     VAPGINNTDAVNVGQLNNVVSNVGKSIQNLNNRINHVEKNANAGIAEAMATAGLPQAY
                     LPGKSMMAIGGGSYSGETGYAIGYSTISDNGRWMFKGTGSGNSRGNYGGTLGVG"
BASE COUNT          273 a          125 c          202 g          217 t
ORIGIN      
        1 tggtagccag aatgcaacaa taagtattgg taatagtaat tacaaacggc agataaaaaa
       61 tgtagagaat ggtattgatg attatgatgc tgttaacgtt tctcagttaa aggctgtcag
      121 taatagtgca tctgaggcta ttagcattgt caataatgct aatactagta taaaccaggc
      181 ggttaatata gctaacagtt taaacgatac ggtcagtaaa ggaataaata taggtgctga
      241 taatggtcct gtagtaaaac gagaattagg agatacagtt actgtaaccg gagacagtaa
      301 tataagaacc aaaaccacca gtaccggggt caaagtcgct ttaaatcaga atattaatct
      361 tggtgagaat ggtagcgtga atactggtaa cacaaccgtt aattctcatg gtgtaattat
      421 taatgaaggc ccgagcatga cgcggagtgg ctttaacgct gctaataata gaatcaccaa
      481 tgtagctcct gggataaata atacagatgc agttaatgtg ggacagctaa ataatgtagt
      541 atctaatgta gggaagagta ttcaaaattt gaataaccgc atcaaccatg tggagaaaaa
      601 tgcaaatgca ggcatagctg aggctatggc cacagccgga ctgccgcagg cttatctgcc
      661 tggtaagagt atgatggcga taggtggcgg ttcttatagt ggtgaaacag gctatgcaat
      721 tggctattcc accatatcgg ataatggcag atggatgttt aaaggtactg gcagtggtaa
      781 ttcacgtggt aactatggtg gaacccttgg tgttggt
//
LOCUS       DAPPNJ010000415          815 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000415 DAPPNJ010000000
VERSION     DAPPNJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.418"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            251..793
                     /locus_tag="RPM73_004548"
                     /pseudo
     CDS             251..793
                     /locus_tag="RPM73_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          226 a          158 c          214 g          217 t
ORIGIN      
        1 cataccgtta ttttgatgca ggtaaatccc agattactta cagaaatgaa tggaatgagg
       61 aatttaaatc cagagtttca gtcagaagta atgatattat gcttggcgtg acatatgatt
      121 tctgacatac catattgaaa tattacatta gtttatcaaa atgttataaa attaccggat
      181 gttctgggag tggaacatcc ggtttgtgcc taatgaggga attgtggcac tccgttactg
      241 ttccagtcga attgcggata atactcaact aatcagtcta acctgcggtt gttcacaatc
      301 accgacaccg taaatggcgt ccccctgata tctgccagtt catgacatta gtcgaagtcg
      361 gtgttgcgtt atgtgccagg agcatggttg tcgggagact gcctgtcccg tgggctggtt
      421 ccggcaaccg gtatactctg tggttcgaat tgttcgtgct ctcatggtta aaaatcagta
      481 ctgttgaagg cgtcaaaaac cagttggaat gctgttgacg gcattatgac acgtgcggtt
      541 aagtgcggcc tgtcccggat aatccgtatc ttcctggtga aagtcgttat caggaaaagg
      601 gaatttgctt ccggtggcag tacagcgata agtggatgac caaatccagg caggaaaagc
      661 tgatatggct gcgtacgcag atgaagctga cgagccattt cctggcgcga tgaaagaact
      721 ggcaaaggat atcaggagca gaccatgtat cgaggaaaga cgcaatgact ggcagagatg
      781 gatggcgttg gcggtcaaca tggaaaatcc atgaa
//
LOCUS       DAPPNJ010000416          801 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000416 DAPPNJ010000000
VERSION     DAPPNJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.419"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(72..800)
                     /locus_tag="RPM73_004549"
     CDS             complement(72..800)
                     /locus_tag="RPM73_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDX6522783.1"
                     /translation="MKKVFAVSVVGAACVFAVNAGAEEGKSGFYLTGKAGASVVSLSH
                     QRFLSGNEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGNADTKY
                     NLYKDDWSRGDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTGISTW
                     DYGYGYSGRESLSRSGSADNFAWSLGAGIRYDVTPDIALDLSYRYLDAGDSSVSYKDE
                     WGDKYKSEVDVKSHDIMLGVTYNF"
BASE COUNT          221 a          209 c          156 g          215 t
ORIGIN      
        1 gtttaattgc agaagatata caattatatt aattacaaaa tattcgtgat tatctctcgg
       61 gaacagtgtt gtcagaagtt ataagtcaca ccaagcatga tgtcatgact tttaacatca
      121 acctctgatt tatatttatc gccccactcg tccttgtaac tcacactgct gtcacccgca
      181 tcaagatagc gatagctgag gtccagagcg atatccggag taacgtcata gcggataccc
      241 gcgccaaggc tccatgcgaa gttgtcagca gagcctgaac gtgacaacga ttcacgacca
      301 ctgtatccgt acccgtaatc ccaggtactg ataccggttg ttttctggtg aattctggcg
      361 tagccaatcc ctgcagatac ccatggtgtg aatgcgctgt cattccggaa gtcatagtac
      421 gcattcagca tcagagtgtt gactgacacc tcattcttca ggtcaccacg cgaccagtcg
      481 tctttataaa gattatattt cgtgtcagca tttccacgag cataaaactc cagttctgta
      541 cgaaccggaa tactgaactg tggataaaaa tcataaccgg ctgcgatacc gccactgaat
      601 accgtgtcat ggtcatcccc acctttatac tttgacgttt cttcttcatt tcctgacagg
      661 aaacgctggt gtgaaagtga caccacagag gcaccggctt taccggtcag ataaaaaccg
      721 cttttacctt cctcagcacc tgcatttact gcaaatacac aggctgcgcc caccactgaa
      781 acagcaaaaa cctttttcat t
//
LOCUS       DAPPNJ010000417          795 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000417 DAPPNJ010000000
VERSION     DAPPNJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.420"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            187..795
                     /locus_tag="RPM73_004550"
     CDS             187..795
                     /locus_tag="RPM73_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDX6522784.1"
                     /translation="MEKEYGTAVAQELVTGLLSGQDYIKRNPDTEAMAKAQSIMNTWG
                     YHKSNASIGDAPLLFGGSVLGLTVKGMATNAAIGISVNSAAQLAGKDPFSYVDAIIAG
                     VTAAATTGKGIIVSTPINMSGAAIGSSIKGEDPTNSVIGTGLGSVFGGGIGKVISGSM
                     GSTVKEGTSDIIGNIGGSITGEAIGNVTKGTLDEADKSIKNK"
BASE COUNT          263 a          127 c          208 g          197 t
ORIGIN      
        1 gataaagcca ttgagaaaat caggaacggc gataagacat tagccactgc taatgagctg
       61 attaagctgg aaaatgcaga taaacgtagt gatgctctgg tatacaaatt taataaagat
      121 ccttcacaat taaacagcgc agaacgtgct gaactggcag cttacttgcg tgtatatgct
      181 tcagaaatgg aaaaggagta cggtacagcg gtggcacagg agcttgttac tgggttactg
      241 tcaggacagg actatataaa acgtaaccct gatactgaag caatggcaaa ggctcaaagc
      301 attatgaata cctgggggta tcataaatct aatgcaagta ttggtgatgc tcctttgttg
      361 tttggaggaa gcgtactggg gttaacagtg aaaggtatgg cgactaatgc agcaattggt
      421 atcagcgtaa actcagctgc tcagcttgct ggaaaagatc ctttcagtta tgtggatgcg
      481 ataattgcag gggtaacggc agcagcaaca acaggaaaag gaattatagt ttcaacccct
      541 atcaacatga gcggcgcggc aattggtagt agtattaaag gcgaggatcc gacaaattca
      601 gttattggga caggattagg ttcagtattt ggtggcggta tagggaaagt catctcgggg
      661 tcaatgggct ctactgtcaa agagggaaca tctgatatta ttggtaatat tgggggatca
      721 attactggtg aagctatcgg gaatgtaaca aaaggaacac tagatgaagc tgataaatca
      781 attaaaaata aataa
//
LOCUS       DAPPNJ010000418          792 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000418 DAPPNJ010000000
VERSION     DAPPNJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.421"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>792
                     /locus_tag="RPM73_004551"
     CDS             <1..>792
                     /locus_tag="RPM73_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522785.1"
                     /translation="NDGSHSFPDNTLDILRRSTAQRLKKKFTFQADPFLLEKEDKLYI
                     FYEAFNFRNSKGILRCRILDKNLKELDDVKLEGFDNLKCHLSFPFLFTTNGTLYMIPE
                     SSERKEVILFQAIDFPVRWKQVKVLLSDAPLTDNVLLTVNERFYLMSTSMENELVIHT
                     APEVTGKWEKITPLLEVCNEHNRSAGGPYTIDNKMYILTQECTPDTYGKSVFIKELNN
                     LDISTFKESLVDNIPPSLNNSDGVHTLNFSDNYIVYDTKKNVFSP"
BASE COUNT          260 a          155 c          147 g          230 t
ORIGIN      
        1 aaaacgatgg ttctcattcc tttcccgata acactctgga catactgaga cgttcaacag
       61 cacaaagatt aaaaaagaaa ttcacttttc aggccgatcc gtttcttctt gagaaagagg
      121 ataagctgta tattttttat gaggcattta attttcgcaa ctcaaagggt attctgagat
      181 gccgtatcct ggataaaaac ctcaaagaac tggatgatgt gaaactggag gggtttgata
      241 atttaaaatg ccatctgtca tttccattcc tgttcaccac aaatggaact ttatatatga
      301 tcccggaatc ctcagaaaga aaagaggtga tattattcca ggcgatcgat ttcccggtgc
      361 gctggaaaca agtcaaggtt ttactctcag acgctccgct taccgacaat gtgttactta
      421 ctgttaatga acgcttttat ttaatgtcga cctcgatgga aaatgaattg gtcattcata
      481 ccgcgcctga agtaactgga aaatgggaaa aaataacgcc attacttgaa gtctgtaatg
      541 aacacaatcg tagcgcgggt ggtccgtata ccatcgataa taaaatgtat atcctgacgc
      601 aggaatgtac gccagatact tatggaaaat cggtttttat caaggagtta aataatttag
      661 atatatcaac atttaaagaa agcctcgtag ataacatccc tccatctctc aataacagtg
      721 acggagtaca tacacttaac ttttccgata actatatcgt ttatgacacc aaaaaaaatg
      781 tctttagtcc at
//
LOCUS       DAPPNJ010000419          779 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000419 DAPPNJ010000000
VERSION     DAPPNJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.422"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..456)
                     /gene="tnpA"
                     /locus_tag="RPM73_004552"
     CDS             complement(<1..456)
                     /gene="tnpA"
                     /locus_tag="RPM73_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HDX6522786.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFNSGG"
BASE COUNT          204 a          198 c          141 g          236 t
ORIGIN      
        1 tcctccagaa ttaaacgggc tgcccggata gggaatcgat aactgctctc ccattttatc
       61 ctcttcaagc tggtgcttta tgtaatcctg tatcttcgcc gtgttcttac ccaccgtatc
      121 gacgtagtac cctctgcacc agaactccct gttcctgtat ttgaatttca aatcaccaaa
      181 ctgctcgtaa agcatcagac tgcttttccc tttcagatat cccataaagc cggatacgct
      241 cattttgggc gggatctcca caagcatatg gatatgatct gcacagcatt cagcttcaag
      301 aatccgtacg cttttccact cacacagctt tctcaaaata cagcctattg ctctacgctt
      361 ctctctgtag aacacttgtc ttcggtattt tggcgcaaaa actatgtgat atttacagtt
      421 ccatcgggtg tgcgctaagc tcttttcgtt ccccattggg accccctttt gatttcttgt
      481 ttgacacttg cagttgccag accgcaagat gttttaacaa atcaaaggag gttttaataa
      541 ctggctcaaa gctgaaagct ttccggaacc cccagcctgg ctgagggttt tctgtgcaca
      601 aaaatacccg cacaggatgt gcgggtacca atagcacgta aagttatcag atgtacattt
      661 aattataata atgcttgtac actttttatt atttacactg cacgctatag atactgtgaa
      721 ttttatcaca aatcatacct accagaaaaa agcccgcaac tatgcgggca atgacgtta
//
LOCUS       DAPPNJ010000420          768 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000420 DAPPNJ010000000
VERSION     DAPPNJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.423"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..654
                     /gene="lpxT"
                     /locus_tag="RPM73_004553"
     CDS             <1..654
                     /gene="lpxT"
                     /locus_tag="RPM73_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDX6522787.1"
                     /translation="SWYIPVNHGFWLPIDADIFYFFNQKLVESKAFLWLVALTNNRAF
                     DGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTAVVLNQLGQALIPVKRASPTL
                     TFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAFMWRYFGKVAGLIALIIFVVF
                     AFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDRLITFFDKSLPGKNKHFQNK"
BASE COUNT          189 a          165 c          170 g          244 t
ORIGIN      
        1 tcctggtata ttcccgttaa tcatggattc tggttgccaa ttgatgcgga tattttttat
       61 ttctttaatc agaaactggt cgaaagtaag gcctttttgt ggctggttgc attgaccaac
      121 aatcgcgctt tcgacggttg ttctctgctg gcgatgggaa tgttgatgct gagtttctgg
      181 ctgaaagaaa acgcccctgg cagacgacgt atcgtgatta tgggtctggt catgctatta
      241 actgcagtgg tattaaacca gctgggtcag gcattaattc ctgtaaaacg ggccagccca
      301 acattgactt ttaccgatat taaccgcgtc agcgaactgc tctctgttcc cacgaaagat
      361 gcctcacgag atagctttcc cggcgatcac ggcatgatgc tgcttatttt ttcggcattc
      421 atgtggcgtt atttcggcaa agttgcaggc cttatcgccc ttattatttt tgtggttttt
      481 gcatttccca gagtaatgat tggcgcacac tggtttactg acatcattgt cggttcgatg
      541 accgtgatat tgatcggttt gccctgggtg ttgctgacgc cattaagtga tcgattaatc
      601 accttttttg acaaatcact accgggaaaa aacaaacatt tccaaaacaa ataactcaca
      661 gtaattaaca tcatcaggct tatttttata gtgaggataa tcctgatgat gcgcaccgtg
      721 ctttcatcca tcgcgcgcaa aaatcattct ctaagtaaat gaatggat
//
LOCUS       DAPPNJ010000421          753 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000421 DAPPNJ010000000
VERSION     DAPPNJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.424"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>753
                     /locus_tag="RPM73_004554"
     CDS             <1..>753
                     /locus_tag="RPM73_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HDX6522788.1"
                     /translation="STYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSC
                     LIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQR
                     NLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMI
                     FMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVV
                     SKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIF"
BASE COUNT          204 a          115 c          145 g          289 t
ORIGIN      
        1 tcaacctatc tgatggtaat gattgcttta ttttttatcg atactgttgc atttattttt
       61 atgcagttgt atttcattta tgaccgtagg caattttcaa actgtatact tagtttggct
      121 tttctgagtt gtttgattta ctttgttaaa accgtcatta tcattcagca aattattgag
      181 gggcgtttga caagcagtgt tgtccaaaat gacattgcaa tctattattt gtttcgtcag
      241 atgagtttgt gcatattaat atttctggca ttggtgaata aagttagtga aaacacaaaa
      301 cagcgcaatt tattttccaa aaaaatgact ttgtgcataa gtttgttttt tgtttttggg
      361 gggccgatag ttgctcacat actttctagt cattatgaga gctacaattt acacattgcc
      421 gagttaacca atgaaaatgg tcaggtggtt tggaaagcct catatgttac cataatgatt
      481 ttcatgtggt taaccttgct ttcggttaat ctatacttta atggtttacg ctatgatatc
      541 tggaatggag tgacggtgat tgctttttgt gccgtactgt acaatatctc tctgttattt
      601 atgagtaggt atagcgtgtc cacttggtat attagtcgca ctattgaagt agtcagtaag
      661 ttaaccgtta tggtaatatt tatgtgccat atattcagtg cgctacgagt aacaaagaac
      721 attgcacatc gcgatccctt aaccaatata ttt
//
LOCUS       DAPPNJ010000422          752 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000422 DAPPNJ010000000
VERSION     DAPPNJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.425"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>752
                     /locus_tag="RPM73_004555"
     CDS             <1..>752
                     /locus_tag="RPM73_004555"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDX6522789.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREG"
BASE COUNT          156 a          208 c          218 g          170 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc ttggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact
      361 ttcggcgtga cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat
      421 aaattccagg ccgacactgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc
      481 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      541 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      601 tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt
      661 caaattggta cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac
      721 tatgaattcc tgcgcctcgg tcgtgaaggc ta
//
LOCUS       DAPPNJ010000423          744 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000423 DAPPNJ010000000
VERSION     DAPPNJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.426"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..>744)
                     /locus_tag="RPM73_004556"
     CDS             complement(<1..>744)
                     /locus_tag="RPM73_004556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522790.1"
                     /translation="YDYSNPGIDAKQSILEAIKRTGKAYISGNITLSRFDWPNGAKIE
                     GKSTITYTRRPSVPCNLDSYTPVNHSKMKATWVHGVFDICDMLQLKTAGFNTIIHYGY
                     NFTDGGTFEKACNAAEAVGINVIINSPNDTPPPDIVSLGRRDCVIGYYLFDEPQHQGV
                     SVAEQDARINAWRAVTGKSLCIADNGDWGYGNDTISKHYDIIFVDYYYIGTVSDVDNK
                     KNAIISFGELKYKSSHAKLIPAVGLFTGDV"
BASE COUNT          234 a          164 c          124 g          222 t
ORIGIN      
        1 acatctccag taaaaagacc aacagcagga atcagctttg catgtgagga tttatacttt
       61 aactcaccaa aagaaataat agcattcttt ttattgtcaa catcagatac tgtcccaatg
      121 tagtaataat cgacaaaaat aatatcataa tgttttgaga tggtatcatt accatatccc
      181 caatcgccgt tatcagcaat acaaagtgac ttccctgtaa cagctctcca tgcattgatt
      241 ctggcatctt gttccgcaac tgaaaccccc tgatgctggg gttcatcaaa taaataataa
      301 cctattacac agtcccttct tcctaaagac acaatatcag gtggtggcgt atcattaggg
      361 ctattgatta tgacattaat tccaacagct tcagcagcat tgcatgcttt ctcaaatgtt
      421 ccaccatccg taaaattgta tccataatga ataatcgtat tgaaaccagc ggtcttaagc
      481 tgaagcatat cacatatatc gaaaacacca tgaacccatg tagccttcat ttttgaatga
      541 ttcacaggag tgtaactatc aagattacac gggactgaag gcctacgggt ataagtaatg
      601 gtactcttcc cttctatctt ggcaccatta ggccagtcga agcgagacag agttatatta
      661 ccgctaatat aagcctttcc tgttcgttta attgcctcaa gtatcgactg ttttgcatca
      721 atgccggggt ttgaataatc atat
//
LOCUS       DAPPNJ010000424          731 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000424 DAPPNJ010000000
VERSION     DAPPNJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.427"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            42..>731
                     /locus_tag="RPM73_004557"
     CDS             42..>731
                     /locus_tag="RPM73_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522791.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNE"
BASE COUNT          233 a          152 c          162 g          184 t
ORIGIN      
        1 aaatattcta atcattatga caggcgaggg agtgtccaat tatgaattca aaaaagcttt
       61 gttgcatatg tgtgttattc tcgctgcttg caggatgtgc ctctgaatct tctattgatg
      121 aaaagaagaa aaaagctcaa gtcacacaaa gtaatattaa taaaaacact ccccagcaac
      181 tgacagacaa agatttattc ggtaatgaaa ccactctggc cgtatccgaa gaggatattc
      241 aagctgcgct tgatggagat gagttccgcg ttccccttaa ttctcctgta atacttgttc
      301 aatccggcaa ccgcgcaccg gaaaccatta tgcaggagga gatgcgaaaa tattatactg
      361 tttccacatt ctctggtatc ccggacaggc aaaagcctct gacttgtaac aaaaacaaag
      421 ataaaaatga gaacgaggat gttgccagtg ctgagaatat gaactggatg caggcactgc
      481 gttttgtggc tgcaaaagga catcagaaag cgattattgt ttaccaggat acattgcaga
      541 caggaaaata tgactctgcg ctgaaatcaa cagtatggtc cgactataaa aatgaaaaac
      601 tcaccgacgc tatctccctg cgctacctgg tacgtttcac gctggtggat gtggcaacag
      661 gtgagtgggc tacctggtcg ccggtgaatt atgaatataa agtgcttcca ccattgcccg
      721 acaagaatga a
//
LOCUS       DAPPNJ010000425          708 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000425 DAPPNJ010000000
VERSION     DAPPNJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.428"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..252
                     /locus_tag="RPM73_004558"
     CDS             <1..252
                     /locus_tag="RPM73_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDX6522792.1"
                     /translation="LAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYA
                     RLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            311..>708
                     /locus_tag="RPM73_004559"
     CDS             311..>708
                     /locus_tag="RPM73_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDX6522793.1"
                     /translation="MKTITLNDNHIAHLNAKNTTKLEYLNLSNNNLLPTNDIDQLISS
                     KHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTTQPPGLQNFTAIN
                     LDNNQLTHFDATNYDRLVKLSLNSNALESI"
BASE COUNT          253 a          150 c          118 g          187 t
ORIGIN      
        1 ttagctgcga acgtagcaca cgctgccgaa gtctataaca aggatggtaa taaactcgac
       61 ctttatggca aggttaccgc tctacgttat tttactgatg ataagcgtga cgatggtgat
      121 aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa tcaatgatca aatgattggt
      181 tttggtcact gggaatatga ttttaaaggc tataacgatg aagccaacgg ctcgcgcggc
      241 aagaatctct gattgagtta agtgcccatc acaatatgat cctgacctta tattacacaa
      301 tcatcccagg atgaaaacaa tcactttaaa cgacaaccat attgcacatt taaacgccaa
      361 aaacactaca aaactggaat atttaaactt aagcaataac aatttactgc caaccaatga
      421 cattgatcaa ctaatatcat caaaacatct ttggcatgta ttagttaacg tcatcaacaa
      481 tgatccactt gcacaaatgc agtactggac tgcagtaaga aatataattg atgacactaa
      541 tgaagtgacc attgatttat caggacttaa tttaaccact caaccaccag ggctgcaaaa
      601 cttcaccgct atcaatcttg ataataacca actcacacat tttgatgcaa ccaactacga
      661 tagactcgta aagctaagtc tgaatagtaa tgctcttgag tcaataaa
//
LOCUS       DAPPNJ010000426          706 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000426 DAPPNJ010000000
VERSION     DAPPNJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.429"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            18..>706
                     /locus_tag="RPM73_004560"
     CDS             18..>706
                     /locus_tag="RPM73_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX6522794.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKD"
BASE COUNT          237 a          127 c          138 g          204 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
      301 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
      541 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
      601 agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa tcttgaaagc atatctggct
      661 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataa
//
LOCUS       DAPPNJ010000427          690 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000427 DAPPNJ010000000
VERSION     DAPPNJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.430"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            130..690
                     /gene="pagP"
                     /locus_tag="RPM73_004561"
     CDS             130..690
                     /gene="pagP"
                     /locus_tag="RPM73_004561"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDX6522795.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          182 a          134 c          168 g          206 t
ORIGIN      
        1 aacttgaaca agcggaaata aatagagcag ctattcagat tattctttat gttgggtcta
       61 ttaaggttat gttaattgta gctttgctat gctagtagta gatttttgat aaatgtttta
      121 tggtcacaaa tgaacgtgag taaatatgtc gctatctttt cctttgtttt tattcagtta
      181 atcagcgttg gtaaagtttt tgctaacgca gatgagtgga tgacaacgtt tagagaaaat
      241 attgcacaaa cctggcaaca gcctgaacat tatgatttat atattcctgc catcacctgg
      301 catgcacgtt tcgcttacga caaagaaaaa accgatcgct ataacgagcg accgtggggt
      361 ggcggttttg gcctgtcgcg ttgggatgaa aaaggaaact ggcatggcct gtatgccatg
      421 gcatttaagg actcgtggaa caaatgggaa ccgattgccg gatacggatg ggaaagtacc
      481 tggcgaccgc tggcggatga aaattttcat ttaggtctgg gattcaccgc tggcgtaacg
      541 gcacgcgata actggaatta catccctctc ccggttctac tgccattggc ctccgtgggt
      601 tatggcccag tgacttttca gatgacctac attccgggta cctacaacaa tggcaatgtg
      661 tactttgcct ggatgcgctt tcagttttga
//
LOCUS       DAPPNJ010000428          680 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.431, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000428 DAPPNJ010000000
VERSION     DAPPNJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.431"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>680
                     /locus_tag="RPM73_004562"
     CDS             <1..>680
                     /locus_tag="RPM73_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522796.1"
                     /translation="QNAQWADYVGDILRGAQPINQLVPQHPYLNDVPLIDELRHQNTH
                     HVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIF
                     NLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALS
                     ESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLAS
                     FACVSFGF"
BASE COUNT          199 a          133 c          138 g          210 t
ORIGIN      
        1 cacagaatgc acagtgggct gattatgtgg gtgacattct tcggggggct cagccgataa
       61 accagctagt acctcagcac ccttatctta acgacgtccc actcattgat gagttaaggc
      121 atcagaacac tcatcatgtt attctcttga cacttgatgt tgcaaaaaaa atactcagcc
      181 caataacatc atttgattac attcatttta tcactacaca tccttcaggt ataaaagata
      241 cactcgcctg gttagttaac gcgagtaaat taatgactga gtttgatgac aatgggaaaa
      301 ttatatttaa ccttaatgcc ttaaagtata caaaagcaag ttactttgaa atacttggag
      361 agaaatatat aaaaatcaca acatccagcc cctggctttt agaaaagctc ggtaaatata
      421 ttttctcttc acgagcgcct caagttctgg aactcgctat tggctggcga ggagctttat
      481 ctgaatcaat aaaaggagtt aagttctgta tctggttttc agtagcctgg agaaccatag
      541 aatttattat gagttcagag cgagatctgg ttaattttct tggcgatttt tcaatggatg
      601 tggccaaagc agttatagcc ggtggtgttg caaccgctat tggaagcctg gcttcttttg
      661 cctgtgttag ctttggcttt
//
LOCUS       DAPPNJ010000429          673 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.432, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000429 DAPPNJ010000000
VERSION     DAPPNJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.432"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            42..572
                     /locus_tag="RPM73_004563"
     CDS             42..572
                     /locus_tag="RPM73_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDX6522797.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          194 a          154 c          133 g          192 t
ORIGIN      
        1 cgtgggatag cttgactgta aaaattacag gagctacaaa aatgaaccga ttctcaaaaa
       61 ctcaaattta tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg
      121 aactccgtgg ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt
      181 acaatgcggg tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt
      241 atagtgatcc ttctattgtg ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa
      301 tgcacatcat gctttatata accttccttg cacttcctct gctggggatt gctttgatgg
      361 cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa
      421 acagcgaaat taaagcactg ataaaaaata ttcacgaaac ctgggcaaat ataggttact
      481 ttttaatcgc agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata
      541 ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc cctgacaagg aaatgtgaac
      601 catggacatc aacgcgtcat aaatgtttac gcaagtgcac aatgcaccct ttccactatc
      661 aacaaggagg agc
//
LOCUS       DAPPNJ010000430          670 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.433, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000430 DAPPNJ010000000
VERSION     DAPPNJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.433"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..568)
                     /gene="fimE"
                     /locus_tag="RPM73_004564"
     CDS             complement(<1..568)
                     /gene="fimE"
                     /locus_tag="RPM73_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDX6522798.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLI"
BASE COUNT          200 a          170 c          140 g          160 t
ORIGIN      
        1 ttatgagatt atttctttcc catagtccgg caaaacgagc agcattactg gcggtataac
       61 gtacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacgg gtatcggcac
      121 cacgctccgc cagttcataa ccacaagcat gccttaacat atgaggatga gtctgcgtta
      181 cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc
      241 gactcccgcg gcgagaaata aatatagcgt cggtccggtc agcgcctttc cagttagcac
      301 gttcctgggt ccagcgttcc acggcttcac gctcatcaaa acgtaacggg tgaacggtag
      361 aaaatccgtt cttcagtcgg cgaatattta ttcgaccttc attaaggtca agatcctgat
      421 aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac
      481 aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt
      541 taccggtaag ataacgacgt ttactcacaa tagtagtact cctaactgag atatatttga
      601 atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc
      661 acacacggag
//
LOCUS       DAPPNJ010000431          669 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.434, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000431 DAPPNJ010000000
VERSION     DAPPNJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.434"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..328)
                     /locus_tag="RPM73_004565"
     CDS             complement(<1..328)
                     /locus_tag="RPM73_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522799.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTY"
     gene            complement(358..>669)
                     /locus_tag="RPM73_004566"
     CDS             complement(358..>669)
                     /locus_tag="RPM73_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDX6522800.1"
                     /translation="EPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNE
                     DDCRIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
BASE COUNT          155 a          209 c          108 g          197 t
ORIGIN      
        1 tgtaggtatt tcttatttcg ccatcctcat ctggctccca ccctcccgaa cctcctgtac
       61 tgccactgcc caaacagttc ttctcttcat ccgtcaaatc cttcccgaca tttggattct
      121 cttttgatga atcatcattt ccaaacacgc tggaaaaagc accataggga tctcgccaga
      181 attccatttg actttgtgca tttaataatt cggcttcacc ccgcatctcc atacttttct
      241 caatcgcatc cccaacagcc tcgcgattgt tgtatattac tataccacca ccaataacca
      301 ccgctccaac tgccgcaccg actaacatcc ctggagggcc tcctacccag ggcaagatta
      361 cgaaagcccg ctccccgcaa ggactgacgc cagatagttc ctgtccatgg ctgcttttcg
      421 catcttacgt cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat
      481 gtgccgtatc cctgaaaata attccgctgc atttcctctt cttattctgc agtcgtcttc
      541 attcattacc acgtccagac gccagtgcag cttattctcc acgtgccagt gatttcggat
      601 cgctgtggcg aacttctctg cagttaaatc agcagagctg atataatatc tgaccgtcat
      661 ttcgggttc
//
LOCUS       DAPPNJ010000432          647 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.435, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000432 DAPPNJ010000000
VERSION     DAPPNJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.435"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>647
                     /locus_tag="RPM73_004567"
     CDS             <1..>647
                     /locus_tag="RPM73_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX6522801.1"
                     /translation="IVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKFVI
                     ERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTIAN
                     VRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEEGN
                     IVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDAL"
BASE COUNT          210 a          114 c          144 g          179 t
ORIGIN      
        1 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
       61 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
      121 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
      181 attgataaag ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt
      241 ggtcctaatg gctcaatgat tatcgctgag acattgactt caaatgttaa ccgtacgatt
      301 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
      361 agctatatgt ttgacaatac gggtgtgatt gtatttaaag ggacagaccc tgaccatatt
      421 tttgaaattt tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac
      481 attgttattt atactgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct
      541 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
      601 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttga
//
LOCUS       DAPPNJ010000433          637 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.436, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000433 DAPPNJ010000000
VERSION     DAPPNJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.436"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..>637)
                     /locus_tag="RPM73_004568"
     CDS             complement(<1..>637)
                     /locus_tag="RPM73_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX6522802.1"
                     /translation="VPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGIL
                     LLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPP
                     EKMHCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHL
                     KEPMTADSISSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHN"
BASE COUNT          181 a          142 c          110 g          204 t
ORIGIN      
        1 aattatgaat aacctcttcg aatctttgag ttacgccatc ccaactgtag tgttcaaaca
       61 caaagtcctg accttgcttt gcaactgctg ttaattcagg attcgctaac gtttttaaaa
      121 tatcgctgct tatggagtct gcagtcatag gttcttttaa atggaatccc gtagtatttt
      181 ctttgacaaa ttccgtcatc cccccacgcg tactcactaa aacaggctta ccagcaccca
      241 tagcttcaat tgccaccatg caaaatggtt cctggaactg ggaaggaatg acgactaaat
      301 ctgccagtgg gtaataacaa tgcatctttt caggcggtat gcttcctaac ataatgcaac
      361 gatccttcaa acgttttgca atttccctga cattgcgttg atatgcacct ttgtcacttt
      421 tactcatttc tgtataatca ccaatcacaa ctaattttaa atttttgtga gcagcggcca
      481 atttttcgaa tgattgcatc aacagcaata tgcctttgtc agggacgata cgcccagcat
      541 aaaaaataat tttctcttca ggggagatat taagttcgct tctagtgatc ggctgaaaat
      601 tagattgata ggtttctaaa tcaattccat ttggtac
//
LOCUS       DAPPNJ010000434          635 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.437, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000434 DAPPNJ010000000
VERSION     DAPPNJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.437"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            4..564
                     /gene="slp"
                     /locus_tag="RPM73_004569"
     CDS             4..564
                     /gene="slp"
                     /locus_tag="RPM73_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDX6522803.1"
                     /translation="MTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPG
                     LYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDP
                     VNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYG
                     AFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          182 a          142 c          143 g          168 t
ORIGIN      
        1 aacatgacaa aaggtgcact catcctcagc ctttcatttt tgcttgccgc atgtagttca
       61 attccgcaaa atatcaaagg caataaccaa cctgatattc aaaaaagttt tgttgctgtt
      121 cataaccagc cggggttata tgttggtcaa caagcgcgct ttggtgggaa ggttatcaac
      181 gttatcaatg gcaaaacgga tacgttgtta gaaatcgctg tattaccgtt ggatagctat
      241 gcgaagcctg atattgaagc caactatcag ggccgactgc tcgccagaca aagcggcttc
      301 cttgatccag tgaactatcg taatcacttt gttaccatcc tcggtaccat tcagggtgaa
      361 caaccaggct ttatcaataa agtcccgtat aacttcctgg aagtgaatat gcagggcatc
      421 caggtgtggc atttgagaga agtggttaat accacctata acctgtggga ttacggctat
      481 ggtgcattct ggccggaacc gggctggggt gcgccttact acaccaatgc ggtgagtcag
      541 gtaacacctg agctggtcaa ataacaccac cgaaagatgc agatgctttc tcagcatctg
      601 catcatgtat tacataaaat taatatacag taagc
//
LOCUS       DAPPNJ010000435          628 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.438, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000435 DAPPNJ010000000
VERSION     DAPPNJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.438"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..537)
                     /locus_tag="RPM73_004570"
     CDS             complement(<1..537)
                     /locus_tag="RPM73_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDX6522804.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGK"
BASE COUNT          201 a          122 c          139 g          166 t
ORIGIN      
        1 ttttccttct tcaggctcca gtagtaaata atgtatttgg ccaaattgat cggtgagttt
       61 tccttcgcag ggattacctg aagtggcttg atgaccggta attaatgcca tgctaccaat
      121 atattcttct tctgtaatag ctgaactatg gtcgcgtgga atccagggcg caactatttt
      181 accggtataa tgcaccgcaa taatcgtaaa cagtaaactg acaggaacca cgaacaggtt
      241 cgacagtggt gattgccaga ccatgatgca ggcatgctgc aacaggatgc caataagacc
      301 aaaaaaacca gccagcaagc agagaacgac gagagctggc aatcttccga tattgagata
      361 atgaagtgcc tggctaatat gacctgtggt tatggaatca taatggtcaa gatgtgcatc
      421 aagagcaccg gagagcatgt gaccgcagat aagcgctaaa atttctaaaa ggccgatcaa
      481 cagtacaaag gaaatagcga aaagataagg ggtattatag tcggcgaata aaatcatatg
      541 tcactccaat gtccatttag tgacgcaata tttactttgg gatagtttcc ttgcttacat
      601 ttatctttgt taataataac acatgtta
//
LOCUS       DAPPNJ010000436          623 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.439, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000436 DAPPNJ010000000
VERSION     DAPPNJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.439"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..544
                     /gene="ydeO"
                     /locus_tag="RPM73_004571"
     CDS             <1..544
                     /gene="ydeO"
                     /locus_tag="RPM73_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDX6522805.1"
                     /translation="VPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSI
                     FAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQ
                     TTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKE
                     YRCQSHTGMNTGNTMSALAI"
BASE COUNT          184 a          126 c          130 g          183 t
ORIGIN      
        1 tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat
       61 tgcttatctc aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc
      121 ttgcctgtct atatttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct
      181 atccgtttcc gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct
      241 gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca
      301 agagcaaacg acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt
      361 gatacgcgta gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc
      421 ttattttatt tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga
      481 gtaccgttgt caaagtcaca cgggtatgaa tacgggcaac acgatgagtg ctttagctat
      541 ttgattattt gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc
      601 cattccctgc cccgcgttgt ttt
//
LOCUS       DAPPNJ010000437          613 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.440, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000437 DAPPNJ010000000
VERSION     DAPPNJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.440"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
BASE COUNT          211 a           96 c          131 g          175 t
ORIGIN      
        1 ggtatgcttt acctgccata cctgaacggt taaagatatg attgctgccg ttccggttta
       61 tcagttgaaa tacagaatcc tattctataa tgctctgaga atgaaaacat ctgcgtatac
      121 aattacagaa tgtgcactga cgcctgcgga ggaaatatta gaggacttga catctcatta
      181 ttatatccgc gttatgagtg aaaccagaaa gtatatggac agttgattac tgaagattaa
      241 taatcattta acccatttgc tgaagaaaaa acttaatgac tttattttga ttacgataat
      301 tctgacagag ttattagcat aaaactcagg tatcagcaag atgagcaagg aggcatctgc
      361 agcaacaggt gctacaggtg ttccaggtaa ggataccgaa accagataat ggtcggtatg
      421 gggaattgca gagatttacg ggcatatgac ctgaagaatg aagtatgcag gggaatataa
      481 ggcaccagaa atataaaata acattaataa ataatttaaa tttcatgaaa catgaacaca
      541 gcggcatccg ggtataacgg aatagcgatg ttatattcac agtgttactg tttgtatcat
      601 tttgtgatat gtt
//
LOCUS       DAPPNJ010000438          613 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.441, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000438 DAPPNJ010000000
VERSION     DAPPNJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.441"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            167..>613
                     /gene="sulA"
                     /locus_tag="RPM73_004572"
     CDS             167..>613
                     /gene="sulA"
                     /locus_tag="RPM73_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDX6522806.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVS"
BASE COUNT          160 a          136 c          150 g          167 t
ORIGIN      
        1 gttaactacg aaaataggca acttattctt aaggggcaag attaatttat gttttcccgt
       61 caccaacgac aaaatttgcg aggctctttc cgaaaatagg gttgatcttt gttgtcactg
      121 gatgtactgt acatccatac agtaactcac aggggctgga ttgattatgt acacttcagg
      181 ctatgcacat cgttcttcgt cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac
      241 ggaaaacact acagccgggc ttatcagtga agttgtctat cgcgaagatc agcccatgat
      301 gacgcaactt ctactgttgc cattgttaca gcaactcggt cagcaatcgc gctggcaact
      361 ctggttaaca ccgcaacaaa aactgagtcg ggaatgggtt caggcatctg ggctaccctt
      421 aacgaaagta atgcagatta gccagctctc cccttgccac actgtggagt caatggttcg
      481 cgctttacgc acgggcaatt acagtgtggt gatcggttgg ttggcagatg atttgactga
      541 agaagagcat gctgaacttg ttgatgcggc aaatgaaggt aacgctatgg ggtttattat
      601 gcgtccggta agc
//
LOCUS       DAPPNJ010000439          598 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.442, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000439 DAPPNJ010000000
VERSION     DAPPNJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.442"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            11..>598
                     /locus_tag="RPM73_004573"
     CDS             11..>598
                     /locus_tag="RPM73_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDX6522807.1"
                     /translation="MTFIMRKKKVSLILGILLLAGCGSGTVIKNDGTTDAPKWHKWNK
                     VILNNHLGTFPNLQSLNQVREGMTKDQLYYLIGRPQYDDGWRPQEWNYLFHFYTPGKG
                     VNNVTTCQYKILFDSKMIARSFYWHPVIPEDSVCPPQKSSASQKFTQRYTLNADALFP
                     FDKSDASDINQQGIVSLDNLAMKLNSFEQLNSIRVI"
BASE COUNT          193 a          107 c          122 g          176 t
ORIGIN      
        1 aggaattaca atgactttta ttatgagaaa aaagaaagta tcattaatac ttggtatact
       61 actattggct ggttgtggtt cagggactgt tattaagaat gatggtacaa ccgatgctcc
      121 caaatggcat aaatggaata aagtgatttt gaacaatcac cttggaactt ttcctaacct
      181 gcaaagccta aatcaggtac gtgagggtat gaccaaagac cagttatatt acttgattgg
      241 tcgaccgcaa tacgatgatg gatggcgacc acaggaatgg aactatctgt tccattttta
      301 tactcccggt aaaggtgtta ataatgtcac aacatgtcaa tataaaatct tatttgacag
      361 taagatgata gcccgtagtt tttactggca tccggtaatt ccggaagata gtgtgtgtcc
      421 acctcagaaa tcatcggcat cacaaaaatt tacacagcga tatacgctga atgccgatgc
      481 acttttccca tttgataaaa gtgatgcgtc tgatatcaat caacagggaa tcgttagcct
      541 ggataatctg gctatgaaac tgaattcttt tgagcaactc aatagcatca gggttatt
//
LOCUS       DAPPNJ010000440          598 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.443, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000440 DAPPNJ010000000
VERSION     DAPPNJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.443"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..536
                     /locus_tag="RPM73_004574"
     CDS             <1..536
                     /locus_tag="RPM73_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDX6522808.1"
                     /translation="SLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDL
                     TVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDL
                     LKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKD
                     ATPGNVYAQADYKIAYE"
BASE COUNT          179 a          131 c          138 g          150 t
ORIGIN      
        1 agtcacttct tgttgctggc gtagcaatga ttctttccaa taacgtattt gctgacgaag
       61 gtcacggcat tgttaaattt aaaggtgaag ttatctctgc tccatgttca attaaaccag
      121 gtgacgaaga cttaactgta aatctgggtg aagtagcaga taccgttctg aaaagcgacc
      181 agaaatcttt ggctgagcct ttcactattc acctgcaaga ctgcatgttg agccagggtg
      241 gaaccactta ttcaaaagcg aaggttactt tcaccacagc gaatactatg acgggtcaga
      301 ctgacctgct gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc gttggcgtac
      361 gtattctgga tagccagagc ggcgaagtga ctttaggtac tccggtagta attacattca
      421 acaatactaa cagctatcag gaactgaact tcaaagctcg tatggaatcc ccaagcaaag
      481 atgctacccc gggcaacgtt tacgctcagg ctgactataa gattgcttac gagtaatcac
      541 attaggccgg gttcgcccgg ccacatcatt aacggattaa tgataagtgg aacagatg
//
LOCUS       DAPPNJ010000441          575 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.444, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000441 DAPPNJ010000000
VERSION     DAPPNJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.444"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(100..>575)
                     /locus_tag="RPM73_004575"
     CDS             complement(100..>575)
                     /locus_tag="RPM73_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDX6522809.1"
                     /translation="QLFSVRNLPILALIMGLSFALYGLIRKFIHYDAITSVTLETFWS
                     LPVSLILFIYTDPDVTTPSEIPCILYIMTAPVTVIPLVLFAVALNHTSLIVTGLAQYI
                     EPSMQFLIAVFIFGEVIHYDELFCFSAVWLGLLLCIIESCYRHYRQIPVCKHHTR"
BASE COUNT          202 a           98 c          122 g          153 t
ORIGIN      
        1 tgcctgtagc attactcttt tctctttttt aattataatg gtcagcattt ttatactttt
       61 gaaagtaata aaaaatacct gaaatataac ggatgcttat taccgggtat ggtgtttaca
      121 gacaggaatc tgacggtaat gacgataaca gctttcgata atgcacaaca ataaccccaa
      181 ccagactgca gaaaagcaga ataattcatc ataatgtata acttcaccga atataaacac
      241 agcaatcagg aattgcatag aaggttcgat atattgagca aggccggtta cgataagtga
      301 tgtatgattc agtgcaacag caaacagcac caacgggata acagtgactg gtgcagtcat
      361 aatgtacaga atacatggga tctcagaggg cgtggttaca tccgggtcgg tatatataaa
      421 aaggataagt gaaacaggta atgaccagaa tgtttcaagc gtgactgagg ttatagcatc
      481 atagtgaatg aattttctta tcagtccgta taatgcaaat gaaagcccca tgattagtgc
      541 cagtatggga aggttgcgta ccgaaaacaa ctgaa
//
LOCUS       DAPPNJ010000442          572 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.445, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000442 DAPPNJ010000000
VERSION     DAPPNJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.445"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(15..557)
                     /gene="fimA"
                     /locus_tag="RPM73_004576"
     CDS             complement(15..557)
                     /gene="fimA"
                     /locus_tag="RPM73_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX6522810.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          165 a          122 c          130 g          155 t
ORIGIN      
        1 caacgtggtt ttgattattc gtatctcata ataaaagtcg cgtcagcatt tgcttgccct
       61 gctgatgcac ttgtaccggt gcctttataa cgtgcagaaa aatgaagaac gttggtcccg
      121 gggatcaagt tctggttggt tgagaagcta ttcccatcag gtttgagaat tgcggatgta
      181 ttatcaagta tttcaatacc gacacccgtt gctgttgttg tattggtact ggatgcaatg
      241 gccaataaat tatcattgat tgcatcagcc tgaccagaaa atgcaacggc agcattagcg
      301 gcaacaacag gatcgcagtc gttcaactga atggtgaatg gaattaatgc agaggtatta
      361 ccaacagcat tgaaaatatc ggtacgatat tgaccgagtg tgacaacctg gtctgccgag
      421 tcagtattca ctgaacaggc agcattcacc agttcgcctt caaagtgaat tgtaccgcca
      481 tctacaacgg cagcataact acccgtagcg gcgaatagtg cggcagccag cgcagacgaa
      541 ataaatctta atttcatata tattccttca at
//
LOCUS       DAPPNJ010000443          571 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.446, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000443 DAPPNJ010000000
VERSION     DAPPNJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.446"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            286..519
                     /locus_tag="RPM73_004577"
     CDS             286..519
                     /locus_tag="RPM73_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDX6522811.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
BASE COUNT          209 a          137 c          108 g          117 t
ORIGIN      
        1 aaacattaag ttacgacgaa gaagaacgga aatgccttaa accggaaaat tttcataaat
       61 agcgaaaacc cgcgaggtcg ccgccccgta acctgtcgga tcgccggaaa ggacccgcaa
      121 aatgataata attatcatct gcatgtcaca acgtgcatct acgccatcaa accacgtcaa
      181 ataatcaatt atgacgcaga tatcgtatta attgatctgc atcaacttaa cgtaaaaaca
      241 acttcagaca atacaaatca gcgacactga atacgggaca acctcatgtc aacgaagaac
      301 agaacccgca gaacaacaac ccgcaacatc cgctttccta accaaatgat tgaacaaatt
      361 aacatcgctc ttgagcaaaa agggtccggg aatttctcag cctgggtcat tgaagcctgc
      421 cgccggagac tgtgctcaga aaaaagagtt tcttctgaag caaacaaaga aaagagtgac
      481 attactgaat tgctcagaaa acaggtcaga ccagattgaa gcaatttaga taatcgtgca
      541 gactacgccc cctcatatca tatggaaggt t
//
LOCUS       DAPPNJ010000444          568 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.447, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000444 DAPPNJ010000000
VERSION     DAPPNJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.447"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..>568)
                     /locus_tag="RPM73_004578"
     CDS             complement(<1..>568)
                     /locus_tag="RPM73_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX6522812.1"
                     /translation="PLIRVETENGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRL
                     LDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLA
                     LISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQ
                     GRYITQDPIGLAGGWNLYNYPLNPIIRM"
BASE COUNT           85 a          200 c          143 g          140 t
ORIGIN      
        1 tccatcctta ttatcggatt cagtgggtaa ttatacaggt tccatccccc cgccagccca
       61 atggggtctt gtgtaatata cctcccctgt agcgggtcat agtaccggtt ccggttatag
      121 tacagccctg attcctcatc atgctgctgc cccggcagac ggtatggctg gtgcaggtaa
      181 tacgggttct cttcattaag ctggttgccc cattcatcat actccccgcg ccacgccgta
      241 ttgccgtctt cgctgatgag cgccagcggc aggccccggt ggtcgcagtg gtaaaaatga
      301 acttttcgcg ccggtgtgta ttccggctcc acctgtctgg ccagttgctc caccgtcagc
      361 ccgcactgcg caagccacgc ccggctttca ctgctcacgc ggtctgcccg gatttcttcc
      421 tccagcctgt ccagcagccg caccagttca gccggaaata ccacgccgtg cccgtcctcg
      481 ctcccttcct gctgcagctt ctccgccagg ctgcggcgct gcgctttctc ccgctcgccg
      541 ttctctgtct cgacccggat gagcggcg
//
LOCUS       DAPPNJ010000445          566 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.448, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000445 DAPPNJ010000000
VERSION     DAPPNJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.448"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
BASE COUNT          172 a          112 c          118 g          164 t
ORIGIN      
        1 tgggaaaatg ataaggcaca tagggattag cctatttttg tttatgatca ttgccggcgt
       61 ttttcgccgg cttttttatg tctccagagt tattactttt cgatattacg gtgcttttca
      121 actggtctgc ctgtaaagcc ctggaaaaaa cgaaaggtat ttatgataac cgaattttcg
      181 cgatgacgcc ctgaccacat tatgatttga gtccggacat ctcattgtcg ttatacaaga
      241 agcatgatta tcaatcacgg acattacgac aatgcagcac tgcataccag aactggatac
      301 ctgcgggtcc ggcgacaact ctgcctccct catatataac accagcacac ggacgataaa
      361 gcagtacagg gaagaaatac agtgatatgt aacagcaagc ctgttaatga aaaaagcctg
      421 ccatacggac atatggcagg taagtattta agggaataac cgaagatgcg atagtgttgc
      481 atccctcatt catagtcctt aacaattatg catcaaaata aaaacatata aaggtatttt
      541 gtttcatgag tgttgttttt gtgtgt
//
LOCUS       DAPPNJ010000446          565 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.449, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000446 DAPPNJ010000000
VERSION     DAPPNJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.449"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..560)
                     /locus_tag="RPM73_004579"
     CDS             complement(<1..560)
                     /locus_tag="RPM73_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522813.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTV"
BASE COUNT          149 a          146 c          127 g          143 t
ORIGIN      
        1 tcgacagtaa attgtgaatc agcgttcgcc gtaccagccg ttacggtcgc attggttgcc
       61 acatagcgtg caataaactt catttcagcc ttgccttcag catcgatagc ggtataagca
      121 gagtcgttat aaagtttaac ctgcgaatta tcggactggt taaaaatacg aataccaacg
      181 ccggttgccg caatcgcttg tcctgtaccg gtaaaatcac cagggttgcc tgctgtaagc
      241 ggcgtaccca ctttcaggtc gccgtcgccg ccaggcgctt ctgtaccatc aaaacgaacg
      301 gctgcttgag tgtacgttgc cgggcaattt tcaagcttaa tactgaactt ctgtggcgac
      361 gccagactac ctacacctga aaatgcattt ttactgactt tacccagtgg gacaatttga
      421 tcggctgtat cggaggtaac ttcacaagta gtgtcgacaa tttcaccgac gaagtgaaca
      481 acaccatctt ctgccagtgc atgcccggcc agcattagag atgccagagt gaatgcaccg
      541 cctataatat tacgcttcat aaaaa
//
LOCUS       DAPPNJ010000447          551 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.450, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000447 DAPPNJ010000000
VERSION     DAPPNJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.450"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>551
                     /locus_tag="RPM73_004580"
     CDS             <1..>551
                     /locus_tag="RPM73_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6522814.1"
                     /translation="IETIYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDP
                     LILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNA
                     VNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGY
                     KNHYLSDEMLAGKKELYDFTPES"
BASE COUNT          201 a          108 c           82 g          160 t
ORIGIN      
        1 catagaaaca atctaccgga aaggttatcg tttcagcggg caggttttca aaactaaaat
       61 taatgaagat aatacttcag actattccat agctatattc cctttcacta cttcgttgaa
      121 tacactggat ccattaatac ttaatcagga attagtgcaa atcatttcaa ataaaaaaat
      181 cgatggtctc tatacctatc cgatggctgc gacaaatttt tgtaatgatc acatatctca
      241 aaattcattc ttgagcagat tcaagccaga ttatttcgtt acaggaagaa taaaccagaa
      301 taatgcagtg aacactttat acattgagtt gatcgacgct aaaaaccttt tcctcatcgc
      361 cagtaatcat ctccctgttg atgaactaca taatacatca caatttatta tagataatat
      421 ccttcaaacg gtacataaac cagaacgatc tgtaagatta gctaagcagg accaaggata
      481 taagaatcat tatttatcag atgaaatgtt agccggaaag aaagaacttt acgacttcac
      541 ccctgaaagc a
//
LOCUS       DAPPNJ010000448          542 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.451, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000448 DAPPNJ010000000
VERSION     DAPPNJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.451"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>542
                     /locus_tag="RPM73_004581"
     rRNA            <1..>542
                     /locus_tag="RPM73_004581"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          146 a          113 c          171 g          112 t
ORIGIN      
        1 cacatgctgt gagctcgatg agtagggcgg gacacgtggt atcctgtctg aatatggggg
       61 gaccatcctc caaggctaaa tactcctgac tgaccgatag tgaaccagta ccgtgaggga
      121 aaggcgaaaa gaaccccggc gaggggagtg aaaaagaacc tgaaaccgtg tacgtacaag
      181 cagtgggagc acgcttaggc gtgtgactgc gtaccttttg tataatgggt cagcgactta
      241 tattctgtag caaggttaac cgaatagggg agccgaaggg aaaccgagtc ttaactgggc
      301 gttaagttgc agggtataga cccgaaaccc ggtgatctag ccatgggcag gttgaaggtt
      361 gggtaacact aactggagga ccgaaccgac taatgttgaa aaattagcgg atgacttgtg
      421 gctgggggtg aaaggccaat caaaccggga gatagctggt tctccccgaa agctatttag
      481 gtagcgcctc gtgaattcat ctccgggggt agagcactgt ttcggcaagg gggtcatccc
      541 ga
//
LOCUS       DAPPNJ010000449          540 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.452, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000449 DAPPNJ010000000
VERSION     DAPPNJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.452"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(211..>540)
                     /locus_tag="RPM73_004582"
     CDS             complement(211..>540)
                     /locus_tag="RPM73_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522815.1"
                     /translation="SNNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYLLD
                     KENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTM
                     KITGTPE"
BASE COUNT          164 a          136 c           72 g          168 t
ORIGIN      
        1 tttcattcac caccacttta tatctgtata tttcattaag accttccttg ctaagcggaa
       61 ctaacgtggt aacatacgat ggaagattaa ttatctctat cgttttcacg ctatctattc
      121 cacacaattc attcactcgc cccatcccca gtaccgaacc agaaaataga gacaataaaa
      181 ccatcaaata tttcatcata aaaaacctca ctattctggc gtacccgtta ttttcatcgt
      241 ctgcaacggt ccatccaccc gataaaaagg cagtccacta tgagctcctc tggtacactc
      301 acctaaagtt ttcgccgcac gcgattcaat tattgtaata acccttgcct cttctttatt
      361 ctcatatttc ttatctgttc ggcttaaaag agccatgaaa ttttccttat caagaaggta
      421 atactgcgca tgagcaaact catgtcccaa acctaatgca ggcgattgtg atttcgagtt
      481 taattcagtt gaacaaaata aagatgctct actattccaa tatatcgcca tattattcga
//
LOCUS       DAPPNJ010000450          539 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.453, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000450 DAPPNJ010000000
VERSION     DAPPNJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.453"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>539
                     /locus_tag="RPM73_004583"
     CDS             <1..>539
                     /locus_tag="RPM73_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDX6522816.1"
                     /translation="AEFSIKAMCRVLRVARGGWYTWCQRRTRISTRQQFRQHCDSVVL
                     AAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGL
                     PVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTA
                     QLACDALQMALWRRKRPRN"
BASE COUNT          110 a          154 c          172 g          103 t
ORIGIN      
        1 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc ggcggctggt
       61 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      121 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      181 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      241 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      301 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      361 agaagtgggc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      421 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      481 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaac
//
LOCUS       DAPPNJ010000451          535 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.454, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000451 DAPPNJ010000000
VERSION     DAPPNJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.454"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            99..>535
                     /locus_tag="RPM73_004584"
     CDS             99..>535
                     /locus_tag="RPM73_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDX6522817.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKAT"
BASE COUNT          185 a          103 c          127 g          120 t
ORIGIN      
        1 aggtaaatcc tgattcaccg ttaattatta atatcagctg ataaagataa ttgcatctgc
       61 gggccgtatg gctcgcagtg atttcactgg agaaaaatat gaatatgaag accattgaag
      121 atgtatttat tcacctgctt tcagatacct acagcgcaga aaaacaatta acccgggcac
      181 tggcaaaact cgcaagagca acatcaaatg aaaaattaag tcaggctttt catgcgcacc
      241 tcgaggaaac tcatggacag attgaacgta ttgatcaagt tgtggagtcc gaatcgaacc
      301 tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct tattgaagaa gctaatgagg
      361 tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc actgattgcc gcagcacaga
      421 aagtcgagca ttatgagatt gccagttacg ggacattagc gacgctggct gaacaattag
      481 gttaccgtaa agcagcgaag cttctgaaag aaaccctgga agaagaaaag gccac
//
LOCUS       DAPPNJ010000452          525 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.455, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000452 DAPPNJ010000000
VERSION     DAPPNJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.455"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            218..>525
                     /locus_tag="RPM73_004585"
     CDS             218..>525
                     /locus_tag="RPM73_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522818.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKT
                     "
BASE COUNT          172 a           81 c          135 g          137 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt agtgattata ttaatccaga gcataaaaaa acgat
//
LOCUS       DAPPNJ010000453          517 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.456, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000453 DAPPNJ010000000
VERSION     DAPPNJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.456"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..>517)
                     /locus_tag="RPM73_004586"
     CDS             complement(<1..>517)
                     /locus_tag="RPM73_004586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522819.1"
                     /translation="DNDFYKQARVFNNIFEQESYQYKYYLWGPGLGVGGLIRNLNQTY
                     TSDDVRPWAVLNTGTPTDERHQAFSDSGLAFRNSGGNAALDIETMNSIAISIGYRDHA
                     TDSSTTVEIFYTTDDYYTLTPMRSYTLNNNSGISVCLETAKNIALGLKIAPTTTSAYF
                     FKFLSCSITACS"
BASE COUNT          147 a          102 c           92 g          176 t
ORIGIN      
        1 tgagcatgct gttatcgaac acgataagaa cttaaaaaaa taagcgctgg ttgttgttgg
       61 tgctattttt agtcccaaag ctatattttt agctgtttct aaacaaactg atattccaga
      121 attgttattt agcgtataag aacgcatggg tgttaatgtg taataatcat cagttgtata
      181 gaatatttcc acagtagtgc ttgagtcagt ggcatgatct ctatacccaa ttgaaatggc
      241 aatactattc attgtttcta tatcaagtgc agcattccca ccactatttc tgaatgccag
      301 ccctgaatct gagaatgctt gatgcctttc atctgttggt gttccggtat tcaataccgc
      361 ccatggcctc acatcatctg atgtatatgt ctgattcaga ttcctgataa gtccgccgac
      421 accaagtcct ggcccccaca gatagtattt gtactgataa gattcttgct caaaaatatt
      481 attgaaaact cttgcttgtt tatagaaatc attatct
//
LOCUS       DAPPNJ010000454          515 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.457, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000454 DAPPNJ010000000
VERSION     DAPPNJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.457"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            14..493
                     /locus_tag="RPM73_004587"
     CDS             14..493
                     /locus_tag="RPM73_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDX6522820.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
BASE COUNT          179 a           87 c           85 g          164 t
ORIGIN      
        1 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
       61 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      121 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      181 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      241 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      301 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      361 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
      421 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
      481 tgactttaaa taagacaatt tttagaaata agccg
//
LOCUS       DAPPNJ010000455          500 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.459, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000455 DAPPNJ010000000
VERSION     DAPPNJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.459"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            30..>500
                     /locus_tag="RPM73_004588"
     CDS             30..>500
                     /locus_tag="RPM73_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX6522821.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVD"
BASE COUNT          146 a          103 c          119 g          132 t
ORIGIN      
        1 atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg gcgtttataa
       61 ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc acggatggca
      121 aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag
      181 attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct
      241 ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa
      301 atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc
      361 aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta
      421 catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct gctgagcaaa
      481 cagtgacctt taaagttgat
//
LOCUS       DAPPNJ010000456          500 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.460, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000456 DAPPNJ010000000
VERSION     DAPPNJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.460"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..>500)
                     /locus_tag="RPM73_004589"
     CDS             complement(<1..>500)
                     /locus_tag="RPM73_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX6522822.1"
                     /translation="NSSGDKSDIIYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETF
                     TKEKFDSYVKPVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIR
                     EKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCI
                     FLGDHT"
BASE COUNT          159 a          104 c           77 g          160 t
ORIGIN      
        1 ctgtatgatc cccaaggaag atacaagggg ttatcgcatc gtcctttaaa aaaacatgtt
       61 gatgcaactc attcaagatc atgtctctcg tatttaataa atcgtagagc actttattat
      121 ttttatgttt gtatgcagat gaagccatct cttcgtgttt taataattca cataataaac
      181 tccaaccctt ttccctgatg cctatatgcc ctgaaacgag caatgcataa agtacagcgc
      241 ctactcttcc atctgtatcg ccaaaataag ccgtgttggc attttgtggg ttaatcacac
      301 cacactgttt atagaagtct atttttgaaa acacaggttt aacatagcta tcaaactttt
      361 ccttagtaaa cgtctcttta gagacatccc aacgccagtc aggtttgaaa ttaccactaa
      421 gaagagtaaa gaaatttctc gctatgatag aaagcccatc aactttataa attatatctg
      481 atttatctcc agaggaatta
//
LOCUS       DAPPNJ010000457          498 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.461, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000457 DAPPNJ010000000
VERSION     DAPPNJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.461"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            42..>498
                     /locus_tag="RPM73_004590"
     CDS             42..>498
                     /locus_tag="RPM73_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX6522823.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICM"
BASE COUNT          181 a           90 c           92 g          135 t
ORIGIN      
        1 cgctaaaaac agaatgttat tgccttctag cggaatgcca tatgtctttg gcacttcatg
       61 gaaaaagtga acttgaactt gctgctcaaa aagcattaga gcttttagat tatgtatcag
      121 acataaccac tgtcgatgga aaaattttag ctattatggg actgataact ggtctgtctg
      181 gacaagcaaa agtatctcat atcttatttg aacaggctaa gatacaccca actgatatag
      241 cctctctcta ctactatagg gcactagtcc actttcataa tgaaaaaatt gaagaggcaa
      301 ggatttgtat agacaaatca ctacaactcg aacccagaag acgaaaagca gttgtgataa
      361 aagaatgtgt agatatgtat gtgcctaacc cgctcaaaaa caacattaaa ctctactata
      421 aagaaactga gagtgaaagc catcgagtta taattgacaa cattttgaaa ttaaagcagc
      481 tgacgagaat ttgtatgc
//
LOCUS       DAPPNJ010000458          485 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.463, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000458 DAPPNJ010000000
VERSION     DAPPNJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.463"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>485
                     /locus_tag="RPM73_004591"
     rRNA            <1..>485
                     /locus_tag="RPM73_004591"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAPPNJ010000459          484 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.464, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000459 DAPPNJ010000000
VERSION     DAPPNJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.464"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..374
                     /locus_tag="RPM73_004592"
     CDS             <1..374
                     /locus_tag="RPM73_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDX6522824.1"
                     /translation="DRIYAYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVC
                     QSKKKYQQAYDLYKLSYNYFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDD
                     SVKSKAQAYIELLNDNSEDNG"
BASE COUNT          172 a           73 c           84 g          155 t
ORIGIN      
        1 ttgatagaat atatgcatat gcattcgact accatgaaaa gggtaatgta acggacgcag
       61 aaatttatta taaatttttg tgcatttatg cgttcgaaaa tcatgagtat ctagaaggtt
      121 ttgcatcagt atgtcagtca aaaaagaaat atcaacaagc atatgacctt tacaaactaa
      181 gttacaatta cttcccgtat gatgactatt cagttattta tcgtatgggt caatgtcaga
      241 ttggggctaa aaatatcgat aacgcaatgc aatgtttcta tcacattatt aacaattgtg
      301 aggatgatag tgttaagagt aaagcgcagg catatattga actcttaaac gataattcag
      361 aagataatgg ctaagcatta caatcatttg gttaaatttg ctggttatag tgcagttaac
      421 cactataacc agcacctctt atgttttctt aaatcagcat cttaaaacag ttaagagcat
      481 acta
//
LOCUS       DAPPNJ010000460          483 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.465, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000460 DAPPNJ010000000
VERSION     DAPPNJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.465"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..114)
                     /locus_tag="RPM73_004593"
     CDS             complement(<1..114)
                     /locus_tag="RPM73_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDX6522825.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLL"
     gene            complement(157..393)
                     /locus_tag="RPM73_004594"
     CDS             complement(157..393)
                     /locus_tag="RPM73_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDX6522826.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          147 a           97 c           87 g          152 t
ORIGIN      
        1 gagtaattgc ttaataagag tctgcaatga aaaatttatc ttcatgcgca gtatctggat
       61 caccgcctgg ccagtaattt catattttat acgttcatta tcagatgtct tcataacgac
      121 ttcatagcta acctgttaaa taatcaggct gttgcattat tcaaaaagca acccaattag
      181 tgcattataa tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc aaagaagttt
      241 ggcacaaagc gactttcgac tcagattacg tccatctttc aaaatctcag tcacaatttc
      301 cccgagagtt tcctgctgag taggcaagtt agcttttgca aagtaacgag tgattgctcc
      361 cgcagaatct aaagtcactg aattttgatg catgctacgc ctctgttaaa aatgttaatc
      421 aatgttaagt gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga atggtaaatt
      481 aat
//
LOCUS       DAPPNJ010000461          479 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.466, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000461 DAPPNJ010000000
VERSION     DAPPNJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.466"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(312..>479)
                     /locus_tag="RPM73_004595"
     CDS             complement(312..>479)
                     /locus_tag="RPM73_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX6522827.1"
                     /translation="FLSDTNPTLSEYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLK
                     RMDRRKNKENP"
BASE COUNT          145 a          104 c           76 g          154 t
ORIGIN      
        1 tacattgcat attattacca gaagcgactg gaatactaag cacatcataa aaattatcat
       61 cacatggtat atctgaaaca cacgaaagca tacctaaagt tatactgtat aaacaggtgg
      121 ctaatcccaa ggggatcgat tccagaaatc gggttcaacg gatactgata aaaattccac
      181 ctaccttcca gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg ttaatgttac
      241 cgataatagt tatagtacag ccctgattca tcatcatact actgctgcgg cagtctgatg
      301 aactattgct tttacggatt ctccttattc ttccttctat ccattcgctt tagctttttg
      361 agctcttgaa gtttgctctt tgtatcaata ttaccgttat ttacagcgaa ttccatccaa
      421 taaatagctc tattcaaatt ataatactct gataatgttg gatttgtatc tgataaaaa
//
LOCUS       DAPPNJ010000462          472 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.467, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000462 DAPPNJ010000000
VERSION     DAPPNJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.467"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>472
                     /locus_tag="RPM73_004596"
     rRNA            <1..>472
                     /locus_tag="RPM73_004596"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAPPNJ010000463          462 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.469, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000463 DAPPNJ010000000
VERSION     DAPPNJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.469"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(123..278)
                     /locus_tag="RPM73_004597"
     CDS             complement(123..278)
                     /locus_tag="RPM73_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522828.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(358..423)
                     /locus_tag="RPM73_004598"
     CDS             complement(358..423)
                     /locus_tag="RPM73_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522829.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
BASE COUNT          153 a           94 c           81 g          134 t
ORIGIN      
        1 ttatatggtg caaacgtgta aaagataaga tgaaacatcg caataatcaa catacgatag
       61 tctaaatttt acacaaacag acaaagaaaa ttttcctgaa ttatcaatac aatagcatca
      121 aatcaactca agagccttat tgctgcttcc agaatttctt ctgaagtaac atgtcgatcc
      181 gcggctacat aaatgacttt atgatctccg gtcagagatg gaaaccctgc ggccattaca
      241 gtaaggtgtg ttttttcacc atttggatat tcacgcatga tggtgttaac tccagtcatc
      301 gctggcacta ccactgcagg ttcagagtta aaaaaaacta tgattttttt catgatgtta
      361 ccgtagtatg tgagtatcca tcgaatagat accaagcaaa aaagctcccg aaggagcctt
      421 cattttcact tttttaaatc caacgacaga cggctggcat tt
//
LOCUS       DAPPNJ010000464          458 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.470, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000464 DAPPNJ010000000
VERSION     DAPPNJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.470"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
BASE COUNT          145 a           94 c           88 g          131 t
ORIGIN      
        1 ttctttaaat tctacgataa aggacgtggc tttaactttg tcggaaatca caacaacaca
       61 acgaataaaa tatagcgagg gggagacgtg ttcatgggca ttcgccaggg atgaataaaa
      121 cagcaacaaa aaaatgcccg tacctctcgt acgggccagc gtcacacatt atcatggagt
      181 tgtcagcggg atttattatt agctttgatg gaatatataa tcagatactg gcgttgttct
      241 gtgattttta tcacaccttt aaatgtatta aatcagccac acatctttat gtgttcacca
      301 gtccaggtaa taccggatgc ccctgcatcc acatccgtaa cagcaaacct gaatcattgt
      361 agtggaataa cctctcagac cagtgatacg gatgggtaac aggatatata ctctttaatt
      421 gtatcagttc aaaaaaagct attatttcac gcatttct
//
LOCUS       DAPPNJ010000465          457 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.471, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000465 DAPPNJ010000000
VERSION     DAPPNJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.471"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            88..444
                     /locus_tag="RPM73_004599"
     CDS             88..444
                     /locus_tag="RPM73_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX6522830.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT          113 a           88 c           97 g          159 t
ORIGIN      
        1 tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga atagactttc
       61 gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt aaaaaattat
      121 gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct gattaacgcc
      181 atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt tccattcctt
      241 tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt tcgtcgtctg
      301 cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat cattggctgg
      361 ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg ttacggaaac
      421 gatcctaagt ttggttcaaa ttaatcttag aattggg
//
LOCUS       DAPPNJ010000466          451 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.472, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000466 DAPPNJ010000000
VERSION     DAPPNJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.472"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>451
                     /locus_tag="RPM73_004600"
     CDS             <1..>451
                     /locus_tag="RPM73_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDX6522831.1"
                     /translation="RINRFGRWEKYYYIQLKGAFLSAIHHQIIQNQLDTETITVSYEF
                     ILCQHLIANTEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGN
                     IEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTY"
BASE COUNT          166 a           77 c           80 g          128 t
ORIGIN      
        1 tcgaataaat agatttggta gatgggaaaa gtattattat atacaactaa aaggcgcttt
       61 tttatcggct attcatcatc agatcattca aaatcaactg gatacagaaa caataactgt
      121 tagttatgaa tttatcctct gtcaacatct tatcgcaaat accgagttca gctatttggc
      181 actccctgaa aattataacc gtttgttttt accaaattca aaaaaccaaa caaataatcg
      241 tttcaaaacg ttaaacagca aagctattgg caggctactt gctgctggtg gcgtatacaa
      301 tgggaacatt gaaggattca gagatactgc ggaaaaactg ggtggagatg caataaaagg
      361 ctatgatcaa atactaaatg aaaaaacagc gggcatagcg atagcaacag catctattct
      421 tttaacaaag cgttctaatg ttgatacata t
//
LOCUS       DAPPNJ010000467          451 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.473, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000467 DAPPNJ010000000
VERSION     DAPPNJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.473"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(41..364)
                     /locus_tag="RPM73_004601"
     CDS             complement(41..364)
                     /locus_tag="RPM73_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDX6522832.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          134 a          111 c          115 g           91 t
ORIGIN      
        1 tttctttaat cagcgaagat cccctcgctg attaaaatat ttagagaaag acagggaagg
       61 cggaatgatg gcactgatat gacaaagtga attccgtatt caaacataag gaacccgcaa
      121 aaaggctaca gttgcctact ggctgaccaa aggggaccgc gtgggtatag ccttcctgct
      181 ggcatgcgcg atcggcgatc ccgcgactga cataacgatc ggtatgggca tgctgcaaaa
      241 aagcctggtc ataaaccaac cgtacaatac ctgttgccgc gtcaacttcg ctcacatggg
      301 cctggcgagt aaccgtacac cccgccagcg ttattaatga aagagtcaga atcagaagtc
      361 tcatggtcca gccagaagag taagagaaag cactatttta cgcgttacag cgctgcgaca
      421 atggccggaa aagacgcctg gcaggcgcta t
//
LOCUS       DAPPNJ010000468          450 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.474, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000468 DAPPNJ010000000
VERSION     DAPPNJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.474"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..196)
                     /locus_tag="RPM73_004602"
     CDS             complement(<1..196)
                     /locus_tag="RPM73_004602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522833.1"
                     /translation="MEDIIFMYAGLIVSSLAAIVGLATSIMHTRKKVKANEELATEMA
                     KSFSELESINKSIGDLEATIK"
BASE COUNT          163 a           93 c           64 g          130 t
ORIGIN      
        1 ttttaattgt cgcttccaaa tcccctatgc ttttattgat agattcaagc tcactaaagc
       61 tctttgccat ttcagttgca agctcttcat tagccttaac ttttttacga gtatgcatga
      121 tagatgtagc taagcctaca attgccgcta aagaagacac tatcaaccca gcatacataa
      181 atataatatc ctccattaca tacctctcgt aaacttaaag gagaaaacag gctcacattc
      241 taccacgtgt ttattaaatc gttcgagaag atgaaatata aatattacag ccagtagtat
      301 caccgtaact acatagtacc aggtatatat aataactttt ggctcactaa ctttaccgta
      361 aagataagcg agaatgaaac cacaaaaaat ccctaagcaa aaaatagctt tatctcgaga
      421 agatacaccg atggaaaaag agtatattga
//
LOCUS       DAPPNJ010000469          442 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.475, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000469 DAPPNJ010000000
VERSION     DAPPNJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.475"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..91
                     /locus_tag="RPM73_004603"
     CDS             <1..91
                     /locus_tag="RPM73_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDX6522834.1"
                     /translation="WSFGYPGAIIFMILAGLTPYLYFKRKNWL"
BASE COUNT          139 a           81 c           84 g          138 t
ORIGIN      
        1 gtggagcttt ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta
       61 tctgtacttt aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt
      121 gtgctgacag cgacatttta gccgagggaa tcgcagataa tataattgtt gtgaataata
      181 tccatgccac gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag
      241 agataataga aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc
      301 cagtatctct tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca
      361 gaataaaact caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta
      421 atgtatagat tatgaactga aa
//
LOCUS       DAPPNJ010000470          436 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.477, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000470 DAPPNJ010000000
VERSION     DAPPNJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.477"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..>436
                     /locus_tag="RPM73_004604"
     CDS             <1..>436
                     /locus_tag="RPM73_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDX6522835.1"
                     /translation="SSLLTGVLETTNEPYAIAKIAGIKLCESYNRQYGCDYRSVMPTN
                     LYGTNDNFHPNNSHVIPALMRRFHEAKELGLNEVVVWGTGTPKREFLYVDDMAAASVY
                     VMELDDEVYKKNTQPMLSHINVGTGVDCSIREMAETMALVVGY"
BASE COUNT          136 a           64 c           98 g          138 t
ORIGIN      
        1 atcttcactt ttaacaggtg tactcgaaac tacaaatgag ccatatgcaa tagctaaaat
       61 tgcaggtata aaattgtgtg agtcttacaa tcgtcaatat ggatgtgatt atcgcagtgt
      121 aatgcctact aatttgtatg gtacgaatga taattttcat cctaataact cacatgtaat
      181 tccagcgctc atgaggcgtt ttcatgaagc aaaggagttg gggttaaatg aagtagttgt
      241 ctgggggact ggaacaccaa aacgtgagtt tttatatgtt gatgatatgg cagctgcgtc
      301 agtatatgtg atggagcttg atgatgaagt ttacaaaaaa aatacgcaac caatgttatc
      361 tcatattaac gtaggtactg gtgttgattg ttctatacgc gaaatggcag aaacaatggc
      421 tttagttgtt ggctat
//
LOCUS       DAPPNJ010000471          435 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.478, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000471 DAPPNJ010000000
VERSION     DAPPNJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.478"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..426
                     /locus_tag="RPM73_004605"
     CDS             <1..426
                     /locus_tag="RPM73_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX6522836.1"
                     /translation="YCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLL
                     ARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQE
                     NALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
BASE COUNT          127 a           79 c          124 g          105 t
ORIGIN      
        1 tattgcgtca tgattatgga tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat
       61 ccggttggcg atcaggtgat aaaaacagtg gtgaatatca ttgggaaaag catacgacca
      121 gatgaccttt tagcgcgcgt cggcggcgaa gagtttggcg tcttgctgac ggacatcgat
      181 actgaacgtg cgaaagcttt agcggaaagg attcgggaaa atgttgagcg tttaactggc
      241 gataatcctg aatacgctat accacaaaaa gtgacgatta gtattggcgc tgttgtgact
      301 caggaaaatg cgttaaaccc gaatgagatc tatcgactgg ctgataacgc actttacgag
      361 gcgaaagaga ccgggcgtaa taaggtggtt gtgagggatg tggtgaattt ttgtgagtca
      421 ccataaagcg gcatt
//
LOCUS       DAPPNJ010000472          429 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.480, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000472 DAPPNJ010000000
VERSION     DAPPNJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.480"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..271
                     /locus_tag="RPM73_004606"
     CDS             <1..271
                     /locus_tag="RPM73_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HDX6522837.1"
                     /translation="IMRKYESEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEK
                     PYEITELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          165 a           54 c          103 g          107 t
ORIGIN      
        1 aataatgcgc aagtatgaaa gtgaaggtaa atataccgtt agaaatttgg ttaaaaataa
       61 agctatagca ttggaattag ctgagatata tgttaagaat cgttatggac aggatgccgc
      121 agaagaagaa aaaccatacg aaatcactga gttaacaaca agttgggttg ttgaaggtac
      181 cattcactca gaccaaattg ctggtggggt ttttattata gaaataggca aaaatgatgg
      241 gagaattctg aattttggcc acggaaaata agtatgtaag gataatcaaa catcgtgtgc
      301 gctgatggca gagtaggtgg aggactccag acagtcaaac gatagaaaaa gatagccttt
      361 atggaggttc ctgcaatgtc aaatacatac cagaaaagaa aggcaagtaa agagtatggt
      421 ttatataat
//
LOCUS       DAPPNJ010000473          401 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.482, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000473 DAPPNJ010000000
VERSION     DAPPNJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.482"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..333)
                     /locus_tag="RPM73_004607"
     CDS             complement(<1..333)
                     /locus_tag="RPM73_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522838.1"
                     /translation="MFFAILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQ
                     TLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMA
                     KGATEQIID"
BASE COUNT          124 a           72 c           72 g          133 t
ORIGIN      
        1 atcaataatt tgttctgtag caccttttgc cattagatct gcaaattgtt caagttgttc
       61 ccaaagctta ttactgaaat gagtgtgatt agctaattgt gcctgcgagg aatcgctaat
      121 ctcagtacgt agtaatttta tttgagcaat aagagaacct tcactatcac cgttaatacc
      181 cgagactaat ttagtcagag tctggttctg ctcttttaat tcatgataga tatgttcagg
      241 tgtaacagat tcagggttat tttctgctaa cgcacttcgt ttattggcaa aaaagaaagc
      301 atccattcca ttaaataaaa tggcaaaaaa catacctaca atgcttgtaa tgaatgctgt
      361 ttttaggcca cctaataata cggggatgct ggtatcaata c
//
LOCUS       DAPPNJ010000474          400 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.484, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000474 DAPPNJ010000000
VERSION     DAPPNJ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.484"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            complement(<1..270)
                     /locus_tag="RPM73_004608"
     CDS             complement(<1..270)
                     /locus_tag="RPM73_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522839.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELT"
BASE COUNT          139 a           80 c           59 g          122 t
ORIGIN      
        1 tgttaattct ctgaatgtta ctaccagatc agggtcggta gtaattatta tctcaaaact
       61 acctgtaagc tcgccataag gaacgactat tgtgttttct ggtaactcag aaaaagcatc
      121 acttcttcct aacgaaacat cattaccaat gataacaagt agacgcccat taagtatttc
      181 atactcgcat ccattcaatg gtccgctcaa caaacgaatg gcataagatt ccaataagtc
      241 ttgtgataag aatttatcat tattttccat gaataccacc cgctaattcc ataagctcta
      301 gtttagagta gtgtaacaag acggaaattt catgaccaaa ttatgatgac acagaataaa
      361 aaaattatat tttcgagaac tattttaaat gcctctatgc
//
LOCUS       DAPPNJ010000475          391 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.485, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000475 DAPPNJ010000000
VERSION     DAPPNJ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.485"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            94..285
                     /locus_tag="RPM73_004609"
     CDS             94..285
                     /locus_tag="RPM73_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDX6522840.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT           98 a          101 c           75 g          117 t
ORIGIN      
        1 cttttttaca taacgaagaa ttgaccattt tgtcctgttg tgccttaatg taagtaccgt
       61 ccacagcgtg ggacatactt caaggaacct tttgtgagtc aggcaaccag tatgcgaaaa
      121 cgacaccgat ttaacagtcg catgacccgt atcgtactgc tcatcagctt tatcttcttc
      181 tttggccgtt ttatctactc gtccgtcggt gcctggcagc accatcagag caaaaaagaa
      241 gctcagcaat ccacactctc cgtcgaatca ccggtacaac gttagcggtt accttctcca
      301 ctttcacaga acataacggc acttcgctgt cggatgcttt tgctgtttgg gattatcaaa
      361 gcggcagata ttctttcatc ttaaatttta c
//
LOCUS       DAPPNJ010000476          389 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.denovo.486, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000476 DAPPNJ010000000
VERSION     DAPPNJ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.denovo.486"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
BASE COUNT          109 a           80 c          104 g           96 t
ORIGIN      
        1 tcaacgaacg aattataacc acctctggac agtgttgcaa ttaagataat gagatttata
       61 atcctgactg ttgggaagtt gcgccggatc gaacccattt caagtcctgg ctaccggacg
      121 ggtggtgccg caggtagtgc tctgatccgg agtgcaacat ggcgactcaa caaacgtaaa
      181 ccagtatcga caggcagtgt aagggtaagg tgctggtgtt gcgctcctta ataaggagag
      241 cggatggaca cgctgacaca aaagttaacc gtgctcattg ctgtactgga gctattagtg
      301 gctctgttac ggttgattga tttgttgaag taacgggcaa attgaaacgc atctaagggg
      361 gaaaaccgct tcccccttta ataaaatga
//
LOCUS       DAPPNJ010000477        15989 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.guided.103, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000477 DAPPNJ010000000
VERSION     DAPPNJ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.guided.103"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            129..1097
                     /gene="psd"
                     /locus_tag="RPM73_004610"
     CDS             129..1097
                     /gene="psd"
                     /locus_tag="RPM73_004610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDX6522841.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGEKDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            1119..4442
                     /gene="mscM"
                     /locus_tag="RPM73_004611"
     CDS             1119..4442
                     /gene="mscM"
                     /locus_tag="RPM73_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDX6522842.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4471..4785)
                     /locus_tag="RPM73_004612"
     CDS             complement(4471..4785)
                     /locus_tag="RPM73_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDX6522843.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(4782..5096)
                     /locus_tag="RPM73_004613"
     CDS             complement(4782..5096)
                     /locus_tag="RPM73_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX6522844.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(5148..6650)
                     /gene="yjeM"
                     /locus_tag="RPM73_004614"
     CDS             complement(5148..6650)
                     /gene="yjeM"
                     /locus_tag="RPM73_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDX6522845.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(6869..7846)
                     /gene="epmA"
                     /locus_tag="RPM73_004615"
     CDS             complement(6869..7846)
                     /gene="epmA"
                     /locus_tag="RPM73_004615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDX6522846.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            8171..9979
                     /gene="frdA"
                     /locus_tag="RPM73_004616"
     CDS             8171..9979
                     /gene="frdA"
                     /locus_tag="RPM73_004616"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDX6522847.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9972..10706
                     /gene="frdB"
                     /locus_tag="RPM73_004617"
     CDS             9972..10706
                     /gene="frdB"
                     /locus_tag="RPM73_004617"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDX6522848.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            10717..11112
                     /gene="frdC"
                     /locus_tag="RPM73_004618"
     CDS             10717..11112
                     /gene="frdC"
                     /locus_tag="RPM73_004618"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDX6522849.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            11123..11482
                     /gene="frdD"
                     /locus_tag="RPM73_004619"
     CDS             11123..11482
                     /gene="frdD"
                     /locus_tag="RPM73_004619"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDX6522850.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            11545..12678
                     /locus_tag="RPM73_004620"
     CDS             11545..12678
                     /locus_tag="RPM73_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDX6522851.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            12767..13300
                     /gene="blc"
                     /locus_tag="RPM73_004621"
     CDS             12767..13300
                     /gene="blc"
                     /locus_tag="RPM73_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDX6522852.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(13297..13614)
                     /gene="sugE"
                     /locus_tag="RPM73_004622"
     CDS             complement(13297..13614)
                     /gene="sugE"
                     /locus_tag="RPM73_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDX6522853.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(13789..13935)
                     /gene="ecnB"
                     /locus_tag="RPM73_004623"
     CDS             complement(13789..13935)
                     /gene="ecnB"
                     /locus_tag="RPM73_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDX6522854.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(14046..14171)
                     /gene="ecnA"
                     /locus_tag="RPM73_004624"
     CDS             complement(14046..14171)
                     /gene="ecnA"
                     /locus_tag="RPM73_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDX6522855.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(14223..14789)
                     /gene="efp"
                     /locus_tag="RPM73_004625"
     CDS             complement(14223..14789)
                     /gene="efp"
                     /locus_tag="RPM73_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDX6522856.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            14831..15859
                     /gene="epmB"
                     /locus_tag="RPM73_004626"
     CDS             14831..15859
                     /gene="epmB"
                     /locus_tag="RPM73_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDX6522857.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4015 a         4209 c         4130 g         3635 t
ORIGIN      
        1 cgcgtaaaaa cttttctgat acggatgact gacaattaag ctaaccgtcg ttagaatcgt
       61 cccctttttt tcaggatccg gcatgtaggc cggatcagga acgacaaaac aatggcctgg
      121 aggctacctt gttaaattca tttaaacttt cgctacagta cattctgccg aaactatggc
      181 ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg atggctgaca aaactggtta
      241 tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag ccggacaccg
      301 ccagctaccg cacctttaac gaattctttg tccgtccgct gcgtgacgaa gtacgcccaa
      361 tcgataccga tccgaatgtc ctggtcatgc ctgccgatgg cgttatcagc cagttgggta
      421 aaatcgaaga agataaaatc ctgcaagcca aaggccacaa ctacagcctc gaagccctgc
      481 tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg tacgtttgtg accacttacc
      541 tctccccgcg tgactaccat cgcgtacaca tgccgtgcaa cggtattctg cgtgagatga
      601 tctacgtacc gggcgatctc ttctccgtta accatctcac ggctcagaac gtgccgaatc
      661 tgtttgcccg taacgaacgc gtgatttgcc ttttcgatac cgaatttggc ccgatggcgc
      721 agattctggt cggggcgacg attgtaggca gcattgagac ggtctgggcg ggcaccatta
      781 cgccgccgcg cgaaggtatc atcaagcgct ggacctggcc tgccggggaa aaagacggtt
      841 ctgtggcact gctgaaaggc caggaaatgg ggcgctttaa actcggctct accgttatca
      901 acctgtttgc accaggtaaa gtgaacctgg ttgagcaact ggaaagcctg tctgttacga
      961 aaattggtca gccgctggca gtatctaccg aaacctttgt tacgccagac gctgaaccgg
     1021 ccccgcttcc tgctgaagag atcgaagcag aacacgacgc cagcccattg gttgacgata
     1081 aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt atcacttttc
     1141 tgatggcctg gtgcctcagt tggggggcgt acgccgcgac ggcccccgat agcaaacaaa
     1201 tctcacaaga actggagcag gcaaaagcgg cgaaacccgc gcagccggaa gtcgtagagg
     1261 cgctccagtc cgccttaaat gcgcttgagg aacgaaaagg ttcccttgag cgcatcaagc
     1321 aatatcaaga agtcattgat aattacccga aactttccgc tactctgcgc gcacaattaa
     1381 acaacatgcg tgacgaaccg cgcagcgtgt cgccggggat gtctaccgac gcgctgaatc
     1441 aggaaattct ccaggtcagc agccagttgc tggataaaag ccgtcaggcc cagcaagagc
     1501 aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact gccgcaacag caaaccgacg
     1561 cccgccgtca gttaaatgag atcgagcgcc gcctgggaac gcttactggc aataccccgc
     1621 tcaatcaggc acaaaatttc gcgttgcagt ctgactccgc acgcctgaaa gcgctcgttg
     1681 atgaactgga actggcgcag ctgtctgcca ataaccgtca ggaattagcg cgcttacgct
     1741 cagagctggc ggaaaaagag agccagcaac tggatgcgta tttgcaggcc ttacgtaatc
     1801 agttgaacag ccaacgtcag cttgaggcgg agcgggcgct ggaaagtacc gaattgctgg
     1861 cagaaaacag cgccgatttg ccgaaagaca tcgtcgcgca attcaaaatt aaccgcgaac
     1921 tatcggcggc actgaatcaa caggcgcagc ggatggatct cgttgcctcg caacagcgcc
     1981 aggccaccag ccagacgtta caggttcggc aggcgctgaa tacgctgcgt gaacagtcgc
     2041 aatggctggg atcgtccaac ctgctcggcg aagcgttgcg ggcgcaggtg gcacggctgc
     2101 cggaaatgcc gaaaccacaa cagcttgata ccgaaatggc gcagttgcgt gtgcaacggt
     2161 tacgttatga ggatctgctt aataaacagc cgctgctacg gcaaattcat caggccgacg
     2221 gtcagccgct gaccgccgag caaaaccgta ttctggaagc acagctacgc actcagcgtg
     2281 agttgctgaa ctcattgttg cagggtggcg acacgctact gctggaactg accaagctga
     2341 aagtctccaa cgggcaactt gaggatgcgc tgaaagaggt gaatgaagcg acgcatcgct
     2401 atctgttctg gacctctgac gtgcgcccga tgaccatcgc ctggccactg gaaatcgccc
     2461 aggatctgcg tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa gccagtgtga
     2521 tgatgctgac cagcaaagag acgattttgc cgctgtttgg cgcgttgatt ctggtcggtt
     2581 gcagtattta ctcgcgccgc tatttcaccc gttttcttga acgttcggcg gcgaaagtcg
     2641 gcaaagtgac tcaggatcac ttctggctga cgttgcgcac tcttttctgg tcgattctcg
     2701 tcgcgtcacc gctgccggtg ctgtggatga cgctgggtta cggcttgcgc gaggcgtggc
     2761 cttatccgct ggcggtcgcg attggcgatg gcgtaacggc caccgtgccg ctgctgtggg
     2821 tagtgatgat ttgcgccacc tttgcccgcc cgaacggctt gtttatcgct cattttggct
     2881 ggccgcgcga acgtgtttcc cgtgggatgc gctactacct gatgagcatc gggcttattg
     2941 tgccgctgat tatggcgctg atgatgttcg ataacctcga cgaccgtgaa ttctccggtt
     3001 cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc accctcagcc
     3061 tgaaaaaggc cgggatccca ctgtatctca acaaagaggg cagcggcgac aacattacca
     3121 accatatgct gtggaacatg atgattggcg cgccgttggt tgccattctg gcgtcggctg
     3181 tgggttatct ggcaacggca caggcgctgt tagcgaggct tgaaacctcg gttgccatct
     3241 ggttcctgct actggtggtt tatcacgtta tccgccgctg gatgctgatc cagcgtcgca
     3301 ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa cgcgcgcgtg
     3361 gcgaagagga agcgcatcat cacagtagcc cggaaggggc aattgaagtc gatgaaagcg
     3421 aagtcgatct cgatgccatc agtgcgcaat ccttgcggct ggtgcgctca attttgatgt
     3481 tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct ttcggcttcc
     3541 tcgaaaatat ttctctgtgg gatgtcactt ccactgtaca gggcgtagaa agcctggagc
     3601 cgattaccct cggtgcagtg ctaattgcca ttctggtatt tatcatcacc acgcagctgg
     3661 tgcgcaacct gccagcgctg ctggaactgg cgattttgca gcacctggat ttaacgccgg
     3721 gtacgggtta cgccatcacc accatcacca aatatctgct gatgctgatt ggcgggctgg
     3781 tcggcttctc aatgattggt attgagtggt cgaaattgca gtggctggtt gccgcgctcg
     3841 gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct ggtctgatta
     3901 tcctgttcga aaaaccgatt cgcattggcg atacggtgac aattcgcgat ctcaccggta
     3961 gcgtgacgaa aattaacacc cgcgccacca ccatcagcga ctgggaccgt aaagagataa
     4021 tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc tctgactcgg
     4081 tcacgcgcgt ggtgttgacg ataccggccc ctgccgatgc caatagcgaa gaagtgacgg
     4141 aaatcctgct caccgcagcg cgtcgctgct cgctggtgat cgacaacccg gcaccggaag
     4201 tcttcctggt ggatctgcaa caggggattc agattttcga gctgcgtatt tacgccgctg
     4261 agatgggtca ccgtatgccg ctacgccatg agatccacca gctgattctg gcaggcttcc
     4321 acgcccacgg tatcgatatg ccattcccgc ccttccagat gcgtctggaa agcctcaacg
     4381 gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg tcgtcaggcg ggaagtttgt
     4441 aagtttgtgt gccggatgaa ccaatccggc tcacaaacaa aactgatagc gcagaaaaaa
     4501 ctgccacagc cagtagccga aaatagccag cgcggtggcg tacataaacc agccgcgttt
     4561 ttttcgccac aaagcgaacc agagaaaagg gaaaaagagc ggtaacgtca ttatcacacc
     4621 aacatcacgg gtatcgtctt cttcgagata catcaacaca tcgcagacgt tgttaatccc
     4681 gcgtccatca atcatccact ctttgtccag cgcggaagtg atggttatcc agaggaacgc
     4741 cacaataaac cagatgcagc acaagacaaa catgcgcgca ctcataaggt aaccggtcgc
     4801 ttaatttcgc tatagcgcat acgaaacaaa tcgccgtatc ccactaattc cccgcgaaaa
     4861 accagacatc ggataagata agcgcaataa atatccggca ggtttcccca ccgtttacca
     4921 tcgcgatctt tgagggtgtt aatggtgcta cccaccacca tcgccacttt acgggtgtgg
     4981 caggcatcga caaaagagag aatcgtctgc tctatttccg ctacatcccc ggcagaaaat
     5041 tgcagcgccc tgatttcatc ttcggttatt gccgggtccc ggctggtgtc atccataaca
     5101 tttcctgtct ttacgcccta tcgtcaggca gacgataggg catcgggtta ttccaccgcc
     5161 cactgcgggt tttttgccac tctgctgcaa tagttctggt aaatcgccat cgctaacagc
     5221 gagaagaaga cagggccgcc aatcatccac aatgtgctgt cccagtctcc ggcttcaacc
     5281 acaggctgaa taatggtgaa gacgttcgca aatgtcacca ccagtacgac aaccactgtt
     5341 gcaatcattg ccgacatatg cgttttgaaa atcacaaatg gtctgtcgag atcctgacgt
     5401 gctttaaaaa atgggaaagc cagcgcgagg aacaggtaag gaagcgtcat agacacgttc
     5461 gccatcagcg tcagcttgtt aaagaacgcc gatgcggtac cgccaccaaa cgaaaccagc
     5521 aggatgaaga cagtaaccaa cccgcactgc atccacatgg cgatagacgg catccccatc
     5581 gcattcaggc gcgtcatcgg ttccggccac aacgctttcg gcgtcccctg gatgatggct
     5641 ttcagcggtg aatagcaaag cgtaaagaac gccccggtat aagcgaggaa catcgaaagt
     5701 ccggtaatac gcgcaaacca tacacccagc gacaatgacg cttcaggtga caaatgcagt
     5761 gcgttaccca gcgtcacccc gaggctcttc atcagcacat aggtaatatt gccgaggtta
     5821 acggaaccat tacttaatac ctgctgccag tttgtgctga caccccataa aaatattgcc
     5881 agcgaataac cgattgaaat aacaatagcg gcaaaaacaa tacctttggc aaagttcttt
     5941 tctggatttt ccgttttatc gaccagacca ccaaccgctt caattccgcc ataggcaaaa
     6001 atagcaaata ccacaaacga tagcattgcc agaccggact gataacctgg gttcggtgat
     6061 gcaaggaaat taatatcctg cgcgaaatgc ccaccattta ataacaaaat agtaatgctt
     6121 actaacagca atactaaatt cagacacatt actgcaatac cgcccaccgc agtaatgcgg
     6181 gcaattttat taatcccctt tgaagcaacg acggtgacca gaatcatcca tgccactgcc
     6241 agcagaccaa ccacctgcgt aggctccagt ccagcaatac gccagtgctg ggtcatgtcg
     6301 ctaccataga ggaatgttga gaacggtacc caaactttcg cggaggtact caccatccag
     6361 atgatataag aggaaaacca cataaacgta ccaatgaagg caaaacgtgg tccgacacta
     6421 ttattcatcc aggaatagat accgccttct tctttgcgat aagcagctcc catttcagcc
     6481 atcattaagg cgaatggaat aaagaataac aatgcagaaa atatataaaa gggaatcgca
     6541 ctataaccca ttaagtaata agccgatggg ctattggcaa atccaaatac ggaagtaaag
     6601 atcatcaata tgagtcctat cagactcatc ttttttatcg tgtgaggcat gaaaccatcc
     6661 ttcatttcag cgcgtgtata acacgtgccc actgaactgc actgcgcaac attgagcagg
     6721 cagcgtaaaa accaatacag aattgatacc tggatgatat caaatatcca gtcttaaatt
     6781 gtggccttta acggaaaaat gaaaacatat gctacagaat ggcgcttatc acgccattct
     6841 tcgctgttaa ttcagtaatt tttcagaatt atgcccggtc aacgctaaag gcgatgactt
     6901 cagccagtgt ctccgcgccc agcgccaaca tcaccagacg atcaacacct aatgccacgc
     6961 cggaacagtc aggcataccg actttcaagg cttcaatcag attctggtca atggggtgct
     7021 gcggcaaacc gcgcgccgcg cgcttacggt tatcttgttc aaagcgttgt tgctgctcac
     7081 gggcatccgt taattcatgg aaaccattcg ccagctcaat acctttataa taaacctcaa
     7141 agcgttcagc gacccgatga tcttcggtac tgatttgcgc cagtgatgcc tggctggctg
     7201 gaaagtggta cacaaaggtc ggtttttctt taccaatatt tggctctacg ccaaaggtaa
     7261 acagcaattg tagcaacgtg tcgcggtctt cttcggtatc agcaacgtta ctcaaatcca
     7321 gtttcgctgc gacttcccgc agttgcgttt tgtcggcaga gagcgggtca atttccagat
     7381 aacgcaagaa agcttgttga taagaaaggc tttctgctgc cgggcagtcc agcacctgtt
     7441 gtaagagatc gtccacctcg ttcatcaacc ggtacatatc atagtgcggt cgataccact
     7501 caagcatagt gaactcaggg ttgtgataac gccccatctc ttcattacgg aagctgcggc
     7561 acagctggaa taccggccca caaccggcaa ccagcaggcg tttcatatgg tattccgggc
     7621 tggtcattaa ccagagattc atcccctgcg aatgcccggg gccaacgaaa cgtgtctcaa
     7681 acgggaccaa atgaatatcg gttaccgtcg cctggctcat acaaggcgtc tccacctcca
     7741 gcactccacg atcggcaaag aaacgacgga tctccgccat aatcgccgcg cgttttaata
     7801 agttaggaat ggatgcgctc ggctgccagg atgccgtttc gctcatagtt aaatctccag
     7861 tttttgacaa gggcacgaag tctactcgca acgcgacggc gagacaaatt ttacgcagga
     7921 atcaaacagc ggtgggcagt gactaaaaaa agcacgatct gatggtttag taattaaatt
     7981 aatcatcttc agtgataatt tagccctctt gcgcactaaa aaaatcgatc tcgtcaaatt
     8041 tcagacttat ccatcagact atactgttgt acctataaag gagcagtgga atagcgttcg
     8101 cagaccgtaa ctttcaggca cttaccctga agtacggggc tgtgggataa aaacaatctg
     8161 gaggaatgtc gtgcaaacct ttcaagccga tcttgccatt gtaggcgccg gtggcgcggg
     8221 attacgtgct gcaattgctg ccgcgcaggc aaatccaaat gcaaaaatcg cactaatctc
     8281 aaaagtatac ccgatgcgta gccataccgt tgctgcagaa gggggctccg ccgctgtcgc
     8341 gcaggatcat gacagcttcg aatatcactt tcacgataca gtagcgggtg gcgactggtt
     8401 gtgtgagcag gatgtcgtgg attatttcgt ccaccactgc ccaaccgaaa tgacccaact
     8461 ggaactgtgg gggtgcccat ggagccgtcg cccggatggt agcgtcaacg tacgtcgctt
     8521 cggcggcatg aaaatcgagc gcacctggtt cgccgccgat aagaccggct tccatatgct
     8581 gcacacgctg ttccagacct ctctgcaatt cccgcagatc cagcgttttg acgaacattt
     8641 cgtgctggat attctggttg atgatggtca tgttcgcggc ctggtagcaa tgaacatgat
     8701 ggaaggcacg ctggtgcaga tccgtgctaa cgcggtcgtt atggctactg gcggtgcggg
     8761 tcgcgtttat cgttacaaca ccaacggcgg catcgttacc ggtgacggta tgggtatggc
     8821 gctaagccac ggcgttccgc tgcgtgacat ggaattcgtt cagtatcacc caaccggtct
     8881 gccaggttcc ggtatcctga tgaccgaagg ctgccgcggt gaaggcggta ttctggtcaa
     8941 caaaaatggc taccgttatc tgcaagatta cggcatgggc ccggaaactc cgctgggcga
     9001 gccgaaaaac aaatatatgg aactgggtcc acgcgacaaa gtttctcagg ccttctggca
     9061 cgaatggcgt aaaggcaaca ccatctccac gccgcgtggc gatgtggttt atctcgacct
     9121 gcgtcacctc ggcgagaaaa aactgcatga acgtctgccg ttcatctgcg aactggcgaa
     9181 agcgtacgtt ggcgtcgatc cggttaaaga accgattccg gtacgtccga ccgcacacta
     9241 caccatgggc ggtatcgaaa ccgatcagaa ctgtgaaacc cgcattaaag gtctgttcgc
     9301 cgtgggtgaa tgttcctctg ttggtctgca cggtgcaaac cgtctgggct ccaactccct
     9361 ggcggaactg gtggtcttcg gccgtctggc cggtgaacaa gcgacagagc gtgcagcaac
     9421 tgccggtaat ggcaacgaag cggcaattga agcgcaggca gctggcgttg aacaacgtct
     9481 gaaagatctg gttaaccagg atggcggcga aaactgggcg aagatccgcg acgaaatggg
     9541 tctggctatg gaagaaggtt gcggtatcta ccgtacgccg gaactgatgc agaaaaccat
     9601 cgacaagctg gcagagctgc aggaacgctt caagcgcgtg cgcatcaccg acacctccag
     9661 cgtgttcaac accgacctgc tctacaccat tgaactgggc cacggtctga acgttgctga
     9721 atgtatggcg cactccgcaa tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga
     9781 cgaaggttgc accgagcgtg acgacgtcaa cttcctcaaa cacaccctcg ccttccgcga
     9841 tgctgatggc acgactcgcc tggagtacag cgacgtgaag attactacgc tgccgccagc
     9901 taaacgcgtt tacggtggcg aagcggatgc agccgataag gcggaagcag ccaataagaa
     9961 ggagaaggcg aatggctgag atgaaaaacc tgaaaattga ggtggtgcgc tataacccgg
    10021 aagtcgatac cgcaccgcat agcgcattct atgaagtgcc ttatgacgca actacctcat
    10081 tactggatgc gctgggctac atcaaagaca acctggcacc ggacctgagt taccgctggt
    10141 cctgccgtat ggcgatttgt ggttcctgcg gcatgatggt taacaacgtg ccaaaactgg
    10201 catgtaaaac cttcctgcgt gattacaccg acggtatgaa ggttgaagcg ttagctaact
    10261 tcccgattga acgcgatctg gtggtcgata tgacccactt catcgaaagt ctggaagcga
    10321 tcaaaccgta catcatcggc aactcccgca ccgcggatca gggtactaac atccagaccc
    10381 cggcgcagat ggcaaagtat caccagttct ccggttgcat caactgtggt ctgtgctacg
    10441 ccgcgtgccc gcagtttggc ctgaacccag agttcatcgg tccggctgcc attacgctgg
    10501 cgcatcgtta taacgaagat agccgcgacc acggtaagaa ggagcgtatg gcgcagttga
    10561 acagccagaa cggcgtatgg agctgtactt tcgtgggcta ttgctccgaa gtctgcccga
    10621 aacacgtcga tccggctgcg gccattcagc agggcaaagt agaaagttcg aaagactttc
    10681 ttatcgcgac cctgaaacca cgctaaggag tgcaacatga cgactaaacg taaaccgtat
    10741 gtacggccaa tgacgtccac ctggtggaaa aaattgccgt tttatcgctt ttacatgctg
    10801 cgcgaaggca cggcggttcc ggctgtgtgg ttcagcattg aactgatttt cgggctgttt
    10861 gccctgaaaa atggcccgga agcctgggcg ggattcgtcg actttttaca aaacccggtt
    10921 atcgtgatca ttaacctgat cactctggcg gcagctctgc tgcataccaa aacctggttt
    10981 gaactggcac cgaaagcagc caatatcatt gtaaaagacg aaaaaatggg accagagcca
    11041 attatcaaaa gtctctgggc ggtaactgtg gttgccacca tcgtaatcct gtttgttgcc
    11101 ctgtactggt aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt
    11161 attctggggc ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat
    11221 cctgctggtg ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga
    11281 gcgcgttctg gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt
    11341 tctgccgctg tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaattca
    11401 cgtgcctgcg ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct
    11461 gattggtatc gttacaatct aacgtatcgc caatgtaaat ccggcccgcc tatggcgggc
    11521 cgttttgtat ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc
    11581 gcctcttgct ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt
    11641 accccgctta tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt
    11701 aaaccttatt actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag
    11761 caaacgttgt ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgac
    11821 gctattgctc gaggggaaat caagttaagc gatcccacaa caaaatactg gcctgaactt
    11881 accgctaaac agtggaatgg gatcacacta ttacatctcg caacctacac tactggcggc
    11941 ctgccattgc aggtgccgga tgaggtgaaa tcctcaagcg acttgctgcg cttctatcaa
    12001 aactggcagc ctgcatgggc gccaggaaca caacgtctgt atgccaactc cagtatcggt
    12061 ttgttcggcg cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaaca
    12121 cgtgtcttcc agccactcaa actcaaccat acgtggatta atgtaccgcc cgcagaagaa
    12181 aagaattacg cctggggata tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta
    12241 gatgctgaaa cttatggtgt gaagtcgacc attgaagata tggcctgctg ggtacgaagc
    12301 aatatgaatc accgtgatat caacgacaaa acacttcaac aagggataca actggcacaa
    12361 tctcgctact ggcaaaccgg cgatatgtat cagggtctgg gctgggaaat gctggactgg
    12421 ccggtaaatc ctgacatcat cgttaacggc agtgacaata aaattgcact ggcagcacgc
    12481 cccgtaaaag cgattacgcc cccaactcct gcagtacgcg catcatgggt gcataaaacg
    12541 ggggcgaccg gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc
    12601 gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctggcagatt
    12661 ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt
    12721 ccttgctgtc atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc
    12781 tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc
    12841 gcgtggcgta acagtagtga ataatttcga cgccaaacgt tatctcggta cctggtatga
    12901 gattgcccgt tttgatcacc gttttgaacg tggactggaa aaagtcaccg caacatacag
    12961 cctgcgtgat gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat
    13021 gtggcagcag agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa
    13081 agtgtcattc tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata
    13141 ccgccatgcg ctggtttgcg gcccggaccg cgactacctg tggatactct cccgcacgcc
    13201 aaccatttct gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga
    13261 tgtcagtaag tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc
    13321 aataatcccc aatacgatta acgccagact cgccaggcgc atcgggttag cggactcacc
    13381 gagcagcaca atgccggtga tggccgcgcc gactgcgcca atacccgtcc acacggcata
    13441 agccgtccct accggtaacg atttcatcgc ccaggcaagt agcgccatac tgactatcat
    13501 cgccgtcaca gtaataacac ttggcgtcaa acgactaaag ccgtgggtat atttcaggcc
    13561 aacggcccat accacttcca gcagaccagc aataactaag ataatccagg acatatcagg
    13621 ctccggagaa ttggggccgt ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc
    13681 tgatggttga acggatattt tgccaggtgg caggggaatt tcaactatta cctttgtgac
    13741 tatcgcaaaa aaacaaacgg cacgacataa ctgtcgtacc gttattgctt attgctgcgc
    13801 tttcgttgct gcaccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg
    13861 cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat
    13921 tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg cctatagcaa
    13981 attaataata gacaacatcg ccaaaaatga tggaaatctg atgatttttc ggaagagaag
    14041 aaaaattagc tggcagcgcg agagatggag ttgccgagat gtttgatgtc ttcgccgaaa
    14101 ccgcgagcgg tgttacagcc cgtgagcagc gtgctggcaa gcaaaacaag aacgataagg
    14161 cgtttcatca tctctgacct gaataagtga tggtgcagcc tgcaggccgc accacaaccg
    14221 cattacttca cgcgagagac gtattcacca gagcgggtat ccactttgat gacttcgccg
    14281 atttgtacga acagcggaac tttaaccaca gcgccagtag acagggtagc cggtttgccg
    14341 ccagtacctg cggtatcacc tttcaggccc ggatcggtat caacgatttc cagttcaacg
    14401 aagttcggcg gagtaacgga gatcggctga ccattccaca gagttacgat acactctgcc
    14461 tgatccagca gccatttagc gttgtcgcca attgcttttg catcagcaga cagctgctcg
    14521 aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc
    14581 atatcgacaa catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga
    14641 gtaccggtca gcagacgacg cagtttaacg cgagcaaatg cctggccttt acccggtttt
    14701 acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt aagaccagca
    14761 cgaaaatcgt tgctatagta cgttgccata aggccctctg aaatttgtta attggtagct
    14821 aagccacaaa atggcgcata ttgtaaccct aaatacccca tccagagaag attggttaac
    14881 gcaacttgcc gatgttgtga ccgatcctga tgaacttctg cgtcttttga atatagacgc
    14941 ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg tttgccctgc gtgtgccccg
    15001 ctcatttatc gatcgcatgg agaaaggcaa tccggacgat cctcttttgc gtcaggtact
    15061 tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc accgacccgc tggaagaaca
    15121 gcacagcgta gtgcctggtt tgttgcataa ataccacaac cgggcgcttt tgctggtcaa
    15181 aggcggctgc gcggtaaatt gccgctattg cttccgtcgc cacttcccct atgccgaaaa
    15241 tcagggcaac aagcgtaact ggcagactgc tcttgagtat gttgctgcgc atccggaact
    15301 ggacgagatg attttctccg gcggcgatcc gctgatggcg aaagatcacg agctggactg
    15361 gttgctcaca caactggaag ccatcccgca tatcaaacgt ctgcggattc acagccgtct
    15421 gccgattgtg atcccggcac gtatcaccga ggcgctggtt gaacgctttg cccgttctac
    15481 gctgcaaatc ttgctggtga atcacatcaa tcatgccaat gaggtagatg aaacgttccg
    15541 tcaggcgatg gctaaattgc gccgtgtcgg tgtcaccctg cttaaccaga gcgttctgtt
    15601 acgtggtgtg aatgataacg cacaaacgtt ggcaaacctg agtaatgcgt tgttcgatgc
    15661 cggcgtgatg ccgtattacc tgcatgtgct agataaagta cagggcgcgg cgcattttat
    15721 ggtgagtgat gacgaagcac ggcagattat gcgtgagttg ctgacattgg tgtcgggata
    15781 tctggtgccg aaactggcgc gagaaattgg cggcgaaccc agcaaaacgc cgctggatct
    15841 ccagctacgc cagcagtaac tgattaaaca gggaagttaa acattagcct gatgcgctac
    15901 gcttatcagg cctacattgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg
    15961 ataaggcgtt tacgccgcat ccggcaaga
//
LOCUS       DAPPNJ010000478         8864 bp    DNA     linear   BCT 25-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC9769
            SAMN37429523-rid19458273.guided.177, whole genome shotgun sequence.
ACCESSION   DAPPNJ010000478 DAPPNJ010000000
VERSION     DAPPNJ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37429523
            Sequence Read Archive: SRR26082377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001893349.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001893349.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/22/2023 19:00:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,636
            CDSs (total)                      :: 4,566
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 47 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37429523-rid19458273.guided.177"
                     /isolate="ARS-CC9769"
                     /isolation_source="Calf fecal composite"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2014"
                     /collected_by="USDA ARS"
     gene            <1..198
                     /locus_tag="RPM73_004627"
     CDS             <1..198
                     /locus_tag="RPM73_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDX6522858.1"
                     /translation="SAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDTLYQYFGQHGD
                     KVVQPLVQKLDKQQVLSQRMR"
     gene            269..1324
                     /gene="dinB"
                     /locus_tag="RPM73_004628"
     CDS             269..1324
                     /gene="dinB"
                     /locus_tag="RPM73_004628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDX6522859.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            1321..1773
                     /locus_tag="RPM73_004629"
     CDS             1321..1773
                     /locus_tag="RPM73_004629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX6522860.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            2019..3158
                     /locus_tag="RPM73_004630"
     CDS             2019..3158
                     /locus_tag="RPM73_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDX6522861.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            3155..3769
                     /locus_tag="RPM73_004631"
     CDS             3155..3769
                     /locus_tag="RPM73_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDX6522862.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(3826..5283)
                     /gene="pepD"
                     /locus_tag="RPM73_004632"
     CDS             complement(3826..5283)
                     /gene="pepD"
                     /locus_tag="RPM73_004632"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDX6522863.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            5544..6002
                     /gene="gpt"
                     /locus_tag="RPM73_004633"
     CDS             5544..6002
                     /gene="gpt"
                     /locus_tag="RPM73_004633"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDX6522864.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            6094..7338
                     /gene="frsA"
                     /locus_tag="RPM73_004634"
     CDS             6094..7338
                     /gene="frsA"
                     /locus_tag="RPM73_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDX6522865.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            7396..7797
                     /gene="crl"
                     /locus_tag="RPM73_004635"
     CDS             7396..7797
                     /gene="crl"
                     /locus_tag="RPM73_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDX6522866.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(7836..8861)
                     /gene="phoE"
                     /locus_tag="RPM73_004636"
     CDS             complement(7836..8861)
                     /gene="phoE"
                     /locus_tag="RPM73_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDX6522867.1"
                     /translation="MGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYMSDNASKDGDQ
                     SYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDY
                     GRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTL
                     QYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRA
                     EAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGY
                     VLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVA
                     VGMTYQF"
BASE COUNT         2179 a         2161 c         2283 g         2241 t
ORIGIN      
        1 agcgcaatgg cggaccagat gctgctggat gccaaaaatc cgcaaagcgc gggcaatcgg
       61 cgtattgaga ttatggtgct gaccaaaagt gcgtccgata cgctgtatca atactttggt
      121 cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc tggataagca gcaggtgctt
      181 tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc tcaaaccctg aaatcactgt
      241 atactttacc agtattgaga ggtgagcaat gcgtaaaatc attcatgtgg atatggactg
      301 ctttttcgcc gcagtggaga tgcgcgacaa tcccgccctg cgcgatatcc ctattgctat
      361 tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc aattatcccg cgcgtaaatt
      421 tggcgtacgt agcgctatgc ctactgggat ggcgctcaaa ttatgcccac atctcacctt
      481 gcttccgggg cgctttgacg cctacaaaga agcctcaaat catatccgtg aaatcttctc
      541 gcgctacacc tcgcgcattg aaccgttgtc actggatgag gcttatctcg atgtcaccga
      601 tagcgtccat tgccacggtt ctgcgaccct catcgcccag gaaatccgcc agacgatttt
      661 caacgagctg caactgacgg catctgcggg cgtggcacca gtaaagtttc tcgccaaaat
      721 cgcctccgac atgaataaac ccaacggtca gtttgtgatt acgccggcag aagttccggc
      781 atttttacaa accttaccac tggcaaaaat ccccggcgtc ggcaaagtct cggcggcaaa
      841 actggaagcg atggggctac gaacctgcgg tgatgtacaa aagtgtgatc tggtgatgct
      901 gcttaaacgc tttggcaaat ttggccgcat tttgtgggag cgtagtcagg ggattgacga
      961 gcgcgacgtt aacagcgaac ggttgcgaaa atccgtcggc gtggaacgca cgatggcgga
     1021 agatatccac cactggtctg aatgtgaagc gattatcgag cggctgtatc cggaacttga
     1081 acgccgtctg gcaaaggtga aacctgattt actgattgct cgccaggggg tgaaattaaa
     1141 gttcgacgat tttcagcaaa ccactcagga gcacgtctgg ccgcggctga ataaagctga
     1201 cttaatcgcc accgcgcgta aaacctggga tgaacgccgc ggcgggcgcg gtgtgcgact
     1261 ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa agacaactgg tgctgggatt
     1321 atgaataaca tacaaataag agactatcag cctggcgatt ttcagcaact atgcgctatt
     1381 ttcattagag cggttacgat gactgccagt cagcattatt caccacaaca aattgccgcc
     1441 tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg cgaaatcaca agtgcgggtt
     1501 gcagtcatta atgcaaaact ggtcggcttt attacctgcg ttgaacacta tatcgatatg
     1561 ttatttgttg accctgaata cacccgccgt ggggttgcca gcgctttgtt aaaacctttg
     1621 attaagtctg aatccgaact tacggtggac gcaagcataa ccgcaaaacc cttttttgaa
     1681 cgttatggtt ttcagacagt taagcagcaa cgcgttgaat gtcggggaga gtggtttatt
     1741 aatttttata tgcgttataa acagcaacat taaatccagc ttgcaatgaa aataacgcaa
     1801 gcctagtatg tgcgcgtttt cttattccac aaactgcaag gaggtaaatc atgacaaatc
     1861 ctttatcaat gactctttgc agacctttcc aggattaatt ctttttttct tgccctggat
     1921 tcgtctgcca tttcctgatt ttttaattgt tactctcaat aattcaagtc gcaacacatg
     1981 caacttgaag tatgacgagt ctatttacgg aatggtatat gggcaattat attcgtccct
     2041 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
     2101 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
     2161 tacatcccgg acgcggctat ccgattggcg cagcgttctt ctctgttggt cgtttttatc
     2221 cggcactggt cggcaatgat atcggctgcg gtatggcgct atggcaaaca gatattctcg
     2281 ctcgcaaata caacgccgat aagtttgaaa agcgattatc agatctggat gacgttgctg
     2341 aagaaagctg gctggaggaa aacctgccgt cagcatttgc acagcatccg tggcgcagct
     2401 cgcttggctc tatcggtggc ggcaatcatt tcgctgaact gcaacaagtt gatcaaatca
     2461 tcaacgctga actgtttgca ctggcaggtc tggatgcaca acatctgcaa ctgttggttc
     2521 atagcggctc gcggggggtg gggcagtcta ttttacagcg gcatattgcc tcgttctcgc
     2581 atcatggttt gcctcaaggc agtgacgacg cgctacggta tattgcggaa catgacgatg
     2641 cgctggcgtt tgcgtgtatt aatcgccaga tgatcgcttt gcgcataatg caacaagtta
     2701 aggccacggg aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcaaatcg
     2761 gcgatcaaca gggctggttg catcgtaaag gtgccacgcc ggatgacaac ggtctggtga
     2821 ttattcccgg ttcacgtggt gattactcct ggattgtaca gcctgttgcg aacgaaaaaa
     2881 cgctccactc gctggcgcat ggggcagggc gtaaatgggg gcgcaccgag tgtaaagggc
     2941 gtctggcagc gaaatacacg gtgacgcaac tctcgcggac tgaacttggc agtcgggtaa
     3001 tttgtcgcga taaacaactc atctttgaag aagcgccaca agcctataaa tcggctgaaa
     3061 gcgtggtgca atgtctggtg caggccggtt taattattcc tgtcgcgcgg ctgcgtccgg
     3121 tgctaacgct caaaaacagt ggagggaaaa aaggatgatc ttgctacaac tctcctcagc
     3181 gcaggggccg gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga
     3241 agctgcccga caagacgtcg cggtaacggt gttggaaaca gaaacgggcc gctactctga
     3301 cacgctgcgt tcggcgctga tttctctgga tggcgataac gcatgggcat taagcgaaag
     3361 ctggtgtggc actattcagt ggatttgtcc gagtccgtat cggcctcatc atgggcgcaa
     3421 aaactggttt ctgggcattg ggcgttttac cgctgatgag caggaacaat cggatgcaat
     3481 ccgttatgag acgttgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc
     3541 ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgtag
     3601 tcagcatgct aacaagcggc tggcacgatt gctgattgcc tggaagctgg agcagcagca
     3661 acaggaaaat agcgcggtgc tgaaatcgca gcggcgaatg ttccatcacc agattgaacg
     3721 tggcaacccg agacggacat ttacagggat ggcttttatc gaaggataat gaaggatgaa
     3781 actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat
     3841 ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg
     3901 ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg
     3961 atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt
     4021 tttgttgaac agacgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc
     4081 cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag
     4141 cgaatccagc atgctcacga cgtagtcttt accgctgtcg atcagtgaac ggatcagaca
     4201 gtgaatttct acgttattat cagtcatggt caccacaccg acgttcaggg aggtttcgac
     4261 cacacctttt gccacgtctg agttgcggat cacaccgttc ggggtggcgt tcagcagacg
     4321 aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag
     4381 caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt
     4441 attcaccaga gatttcagga cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc
     4501 ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcagatccag
     4561 ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac
     4621 gtggatttcc ccgccggagt gacccccttt cagacctttt aaggttaact tgaaggtttc
     4681 aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc
     4741 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
     4801 ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt
     4861 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaactg ccagtgcaga
     4921 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc
     4981 atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg
     5041 cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt
     5101 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
     5161 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
     5221 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
     5281 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
     5341 aaaatgttgt caacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     5401 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     5461 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     5521 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     5581 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     5641 ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     5701 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     5761 tgctaaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     5821 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcacatttt gtgactatct
     5881 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     5941 cctggatcga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     6001 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     6061 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     6121 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     6181 ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc
     6241 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgtgaaat cctcgacgtc
     6301 caggcacgta ttgtgatgag cgatgctgaa cgtaccgatg atgatttata cgataccgtg
     6361 attggctacc gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa
     6421 caaaaagcct gtgcggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct
     6481 acgttgtaca acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg
     6541 caggctttat caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg
     6601 cagatggagt ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa
     6661 ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac
     6721 tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat
     6781 atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat
     6841 cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc
     6901 tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt
     6961 ctgaaagcgg ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc
     7021 cagcaacagg tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat
     7081 gcttccgacg aagcgttgcg cgtggagttg aatcgctatt cattaaaagt gcaaggattg
     7141 ctgggacgtc gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc
     7201 ccggaagagg actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc
     7261 ccatttaacc cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cgactggatc
     7321 gaaaaacgtt tgtgttaaaa atttgctaaa ttttaccaat ttggtaaaac agttgcatca
     7381 caacaggaga ttgcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa
     7441 tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc
     7501 gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga gttctggggc
     7561 tggtggatgg agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg
     7621 ttcgataaag caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga
     7681 ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat
     7741 ctgaagctgg aaccggcgga tgattttcgt gacgagccgg tgaagttaac ggcgtgagtg
     7801 aaatgtgccg gatgcatcac atccggcaat atctattaaa actgataggt catgccaacc
     7861 gcgacaatat catcattatt aatattcaat ttgttatcgc tatccagttg gttgatttta
     7921 taatcaacaa acgctgacat atttttgttg aaataatatg tagccccgac atcgatataa
     7981 ttgaccagat cttcatcacc gataccttca atatctttcc ctttcgataa gacataaccc
     8041 agcgatggac gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc
     8101 ttattggcaa agccgccagt tattggcgtc attttgcgtg tttcagaata aaaagttgcc
     8161 agataaatat tattggcatc gtatttcaga cctgtagccc atgcttctgc acgcttgcct
     8221 gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta
     8281 atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt
     8341 tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca
     8401 tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc
     8461 ataaagttgt cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag
     8521 gcttccacgt catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta
     8581 tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg cggtatcact ctccgcttta
     8641 tttccggcaa actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgtgtt
     8701 tcacctttaa aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc
     8761 atataatgca tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat
     8821 atttctgcag cctgtacaga tgcagatgcc acaatgccca tcac
//
